LOCUS       DANKKG010000001        59163 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000001 DANKKG010000000
VERSION     DANKKG010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59163
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.001"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(77..517)
                     /locus_tag="PW004_000001"
     CDS             complement(77..517)
                     /locus_tag="PW004_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDL2725003.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            complement(646..1572)
                     /gene="dnaI"
                     /locus_tag="PW004_000002"
     CDS             complement(646..1572)
                     /gene="dnaI"
                     /locus_tag="PW004_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HDL2725004.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            complement(1572..2945)
                     /locus_tag="PW004_000003"
     CDS             complement(1572..2945)
                     /locus_tag="PW004_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HDL2725005.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            complement(2968..3459)
                     /gene="nrdR"
                     /locus_tag="PW004_000004"
     CDS             complement(2968..3459)
                     /gene="nrdR"
                     /locus_tag="PW004_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HDL2725006.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            complement(3771..4103)
                     /locus_tag="PW004_000005"
     CDS             complement(3771..4103)
                     /locus_tag="PW004_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDL2725007.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            complement(4155..4784)
                     /locus_tag="PW004_000006"
     CDS             complement(4155..4784)
                     /locus_tag="PW004_000006"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HDL2725008.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            complement(4748..5584)
                     /gene="mutM"
                     /locus_tag="PW004_000007"
     CDS             complement(4748..5584)
                     /gene="mutM"
                     /locus_tag="PW004_000007"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HDL2725009.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            complement(5629..8274)
                     /gene="polA"
                     /locus_tag="PW004_000008"
     CDS             complement(5629..8274)
                     /gene="polA"
                     /locus_tag="PW004_000008"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HDL2725010.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQLTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            complement(8400..9083)
                     /locus_tag="PW004_000009"
     CDS             complement(8400..9083)
                     /locus_tag="PW004_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HDL2725011.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            complement(9076..9561)
                     /locus_tag="PW004_000010"
     CDS             complement(9076..9561)
                     /locus_tag="PW004_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HDL2725012.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            complement(9868..11202)
                     /locus_tag="PW004_000011"
     CDS             complement(9868..11202)
                     /locus_tag="PW004_000011"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HDL2725013.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            complement(11333..12094)
                     /locus_tag="PW004_000012"
     CDS             complement(11333..12094)
                     /locus_tag="PW004_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDL2725014.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            complement(12110..12592)
                     /locus_tag="PW004_000013"
     CDS             complement(12110..12592)
                     /locus_tag="PW004_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HDL2725015.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            complement(12612..14792)
                     /locus_tag="PW004_000014"
     CDS             complement(12612..14792)
                     /locus_tag="PW004_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HDL2725016.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            complement(14936..15667)
                     /locus_tag="PW004_000015"
     CDS             complement(14936..15667)
                     /locus_tag="PW004_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HDL2725017.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            complement(15736..16677)
                     /locus_tag="PW004_000016"
     CDS             complement(15736..16677)
                     /locus_tag="PW004_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HDL2725018.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            complement(16857..17225)
                     /locus_tag="PW004_000017"
     CDS             complement(16857..17225)
                     /locus_tag="PW004_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HDL2725019.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            complement(17836..19227)
                     /locus_tag="PW004_000018"
     CDS             complement(17836..19227)
                     /locus_tag="PW004_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HDL2725020.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            complement(19440..19826)
                     /locus_tag="PW004_000019"
     CDS             complement(19440..19826)
                     /locus_tag="PW004_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HDL2725021.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            complement(19882..20505)
                     /locus_tag="PW004_000020"
     CDS             complement(19882..20505)
                     /locus_tag="PW004_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HDL2725022.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            complement(20569..21237)
                     /locus_tag="PW004_000021"
     CDS             complement(20569..21237)
                     /locus_tag="PW004_000021"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDL2725023.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            complement(21249..21689)
                     /locus_tag="PW004_000022"
     CDS             complement(21249..21689)
                     /locus_tag="PW004_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725024.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            complement(21748..22419)
                     /locus_tag="PW004_000023"
     CDS             complement(21748..22419)
                     /locus_tag="PW004_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725025.1"
                     /translation="MNFLVVLKDTLIERFTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            complement(22604..23002)
                     /locus_tag="PW004_000024"
     CDS             complement(22604..23002)
                     /locus_tag="PW004_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HDL2725026.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(22999..23772)
                     /locus_tag="PW004_000025"
     CDS             complement(22999..23772)
                     /locus_tag="PW004_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HDL2725027.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(23769..24452)
                     /locus_tag="PW004_000026"
     CDS             complement(23769..24452)
                     /locus_tag="PW004_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HDL2725028.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(24619..25506)
                     /locus_tag="PW004_000027"
     CDS             complement(24619..25506)
                     /locus_tag="PW004_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2725029.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(25503..26372)
                     /locus_tag="PW004_000028"
     CDS             complement(25503..26372)
                     /locus_tag="PW004_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2725030.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(26509..27795)
                     /locus_tag="PW004_000029"
     CDS             complement(26509..27795)
                     /locus_tag="PW004_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2725031.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            28068..28646
                     /locus_tag="PW004_000030"
     CDS             28068..28646
                     /locus_tag="PW004_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDL2725032.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            28646..29575
                     /locus_tag="PW004_000031"
     CDS             28646..29575
                     /locus_tag="PW004_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2725033.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            29572..31308
                     /locus_tag="PW004_000032"
     CDS             29572..31308
                     /locus_tag="PW004_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDL2725034.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(31371..32207)
                     /gene="pheA"
                     /locus_tag="PW004_000033"
     CDS             complement(31371..32207)
                     /gene="pheA"
                     /locus_tag="PW004_000033"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HDL2725035.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(32204..32713)
                     /locus_tag="PW004_000034"
     CDS             complement(32204..32713)
                     /locus_tag="PW004_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HDL2725036.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(32706..34001)
                     /gene="aroA"
                     /locus_tag="PW004_000035"
     CDS             complement(32706..34001)
                     /gene="aroA"
                     /locus_tag="PW004_000035"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HDL2725037.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(34014..35105)
                     /locus_tag="PW004_000036"
     CDS             complement(34014..35105)
                     /locus_tag="PW004_000036"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HDL2725038.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(35122..36288)
                     /gene="aroC"
                     /locus_tag="PW004_000037"
     CDS             complement(35122..36288)
                     /gene="aroC"
                     /locus_tag="PW004_000037"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HDL2725039.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(36290..37354)
                     /gene="aroB"
                     /locus_tag="PW004_000038"
     CDS             complement(36290..37354)
                     /gene="aroB"
                     /locus_tag="PW004_000038"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HDL2725040.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(37387..38409)
                     /gene="aroF"
                     /locus_tag="PW004_000039"
     CDS             complement(37387..38409)
                     /gene="aroF"
                     /locus_tag="PW004_000039"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HDL2725041.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(39068..40042)
                     /locus_tag="PW004_000040"
     CDS             complement(39068..40042)
                     /locus_tag="PW004_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HDL2725042.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(40023..41906)
                     /locus_tag="PW004_000041"
     CDS             complement(40023..41906)
                     /locus_tag="PW004_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HDL2725043.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(41924..44149)
                     /locus_tag="PW004_000042"
     CDS             complement(41924..44149)
                     /locus_tag="PW004_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HDL2725044.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            44264..45298
                     /locus_tag="PW004_000043"
     CDS             44264..45298
                     /locus_tag="PW004_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2725045.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(45365..46378)
                     /locus_tag="PW004_000044"
     CDS             complement(45365..46378)
                     /locus_tag="PW004_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL2725046.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(46456..46644)
                     /locus_tag="PW004_000045"
     CDS             complement(46456..46644)
                     /locus_tag="PW004_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HDL2725047.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(47084..47212)
                     /locus_tag="PW004_000046"
     CDS             complement(47084..47212)
                     /locus_tag="PW004_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDL2725048.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(47215..48588)
                     /gene="rlmD"
                     /locus_tag="PW004_000047"
     CDS             complement(47215..48588)
                     /gene="rlmD"
                     /locus_tag="PW004_000047"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDL2725049.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(48699..49754)
                     /locus_tag="PW004_000048"
     CDS             complement(48699..49754)
                     /locus_tag="PW004_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDL2725050.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(49764..51194)
                     /gene="gatB"
                     /locus_tag="PW004_000049"
     CDS             complement(49764..51194)
                     /gene="gatB"
                     /locus_tag="PW004_000049"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HDL2725051.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(51191..52663)
                     /gene="gatA"
                     /locus_tag="PW004_000050"
     CDS             complement(51191..52663)
                     /gene="gatA"
                     /locus_tag="PW004_000050"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HDL2725052.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(52663..52968)
                     /gene="gatC"
                     /locus_tag="PW004_000051"
     CDS             complement(52663..52968)
                     /gene="gatC"
                     /locus_tag="PW004_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HDL2725053.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(53074..55110)
                     /gene="ligA"
                     /locus_tag="PW004_000052"
     CDS             complement(53074..55110)
                     /gene="ligA"
                     /locus_tag="PW004_000052"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HDL2725054.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(55127..57361)
                     /gene="pcrA"
                     /locus_tag="PW004_000053"
     CDS             complement(55127..57361)
                     /gene="pcrA"
                     /locus_tag="PW004_000053"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HDL2725055.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            57516..59075
                     /locus_tag="PW004_000054"
     CDS             57516..59075
                     /locus_tag="PW004_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HDL2725056.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
BASE COUNT        16202 a        12868 c        10508 g        19585 t
ORIGIN      
        1 tcccatatct ccttaaaaaa tatacctttt cgttctataa aacaacattt tcttattttt
       61 gaaaaaatgt tattggttat ttgttcaatg aaacatcaat aatcgaatct ccaggaactt
      121 gcggtgggat agacatcgtc atgatcggca tctcaactaa tgtaaattgg actttgtcac
      181 cgattttcaa ttcatttatg tccacgctta ttttttcagc agtagcattt aagatcactc
      241 cgtcattgct gaatgacgtt cccatattcg ctggatcttc tttggccatg acatcgacga
      301 gtttcaactg aagcgtttct tcttctgctt ctacttgagt gatactttca atcgttccga
      361 caaatatgct ttgtctctcc gttttcgtac tgcttgtttt tactgtttga cccgcatcaa
      421 tcgaatcaac agaacctgtg ttgctatctg cgctaccgca gcctgctaaa agaaaaaata
      481 gaccaactac agatatacta gaaaaaatag ccctcataaa aaatcctccg tttaagtttc
      541 tgcattactt gttgcggtga ttgtctccgg tcatttctgt ccatataatg attcgctgac
      601 ctgcttttac tactaggtca aatgtcagtt ggacttgtca actgttcaac cattgcgtcg
      661 gttgcgacca gtcatttcga tttcttttgt caaataacgt atgcgttcca ttatccgttt
      721 cgcttttagc ggctcttcat ctccacgttg agtgacggac aaatgctgtt ccaattcatc
      781 cattgtaaaa ttcgatgaga agaaggttgg taactgttcc tgcatccgat attgaaggat
      841 aacaccgaat acttcgtctc gtatccaact actcatcgca tctgcaccaa tgtcatcgat
      901 catcaaaata ggtgaacgtt tcactgcatc taacttttct gccacttgat cttttccgat
      961 tgcctgtttc atctcaacgg caaatgtcgg aaaatggact agcgtcgtgg tgaatccagc
     1021 aatcgctaat tctctcgctg ttgctcctaa taaatatgtt tttccaatac caaaacttcc
     1081 tactagatac agtcctttat gatacccttt tggatcagca gtatatttct ctacaaaatc
     1141 tagggcttct aagctcgcat atcctcgtcc atctgtacgt tcaaagtttt caaaagaagc
     1201 cgattgaata tcttttggca tatccattgc ttttactcgg cttctgattt cttcttgtct
     1261 ttgccgtgct aacaatgctt ccgttggtac ataagtgaca tccacataat gaaaattcaa
     1321 cgtcagcttc ggctcatagc cgggagcgat catcgttgga tcgttttttt gatatttacg
     1381 cttttcttgc acgaattcat acaatttagc gtaacttttt tcaatatccg cttgtgtcaa
     1441 ttcttcttgg tgtgcgctta gaaaagcttg aacatccgca tcatttaaga cttcagcgat
     1501 catttcagca tacttttctt gataattgcg tttgctgatg atcctcttca attctttgcc
     1561 tacgtcttcc actagatatc accctccttt tttgccaggt attctttaaa acggcggtca
     1621 atctctgctt ttttctcgct gctgatctgt ttttcatctt ttggctggtt gacccaatca
     1681 ggaagctttt cccgtcgaac atttcgcttg ccagtttgat aatttgtttg cttcttttgt
     1741 tttgattgtt ttgcttgatt acttatttgc cgaatgtgct ggatcgcttc ttctgctgtg
     1801 gcgattttct tttgtgccca ttcatttgcc actgtattta cataactggc atttaagcta
     1861 gcattattct tgatcactag tacataattc agcaaaacat tgatcacgct actggaaaga
     1921 cctgattgag aaacaagatc ttgcagtaac cagttttctt gtttagaaac atatccgcct
     1981 ttttctgact tgattgcttc cagatatttc atcggtggaa tgctctgtgc ttcttttatc
     2041 agctgaagcg ctgcgtttgt caaatgttcg tttgacgagg gttcattact gttttcagaa
     2101 accgcaactg tttcttttgt ttgcttcacc ggacgtctct gcattgccaa aatggctttt
     2161 tgcagttctt tttcagatac tttttgttca ctaaagtcat aagattgact gatcagttcg
     2221 ccaagtgtca attcatcgaa accgtaaagc tgatgataca tgtcgacttg tttgatgatt
     2281 ggatcatcaa gacttgcaat atgttttttt gccagccaat cattcaaaaa ctgccaatcg
     2341 aatgtcgaat ctgcttcaag taatgctact gattcagatt cttcaaaaga tttattcagc
     2401 gattcgatgt tttcttgatt agcagcgtac actgcttcat tgaagcgata aacatctaaa
     2461 aatttttttg tgattttttg atatccctct gctgtatgcc ttttaggttc aaagaattgg
     2521 gtaagcttct ggaatcgtcg ttcaccaact ttttccagaa gaagaaaagc cagaacagag
     2581 tctttgaaaa atgctttagg aatgagtggt tctaacagct cataaataaa acgaggtccc
     2641 agatcactat cttctttaaa ataaacatcc agtaagccga ttccttctag cttttttctt
     2701 gcatcataaa attgcgggat accgcagcct agactactaa gcagatctgc atgcatccct
     2761 tcaggacctt tgccagattc ttgagagatt tctgataaca gggtgaaata gatactcaag
     2821 gcttctccgc cgattagtgg ctgataaaga tacagcaagg cgttcttgct gtcttctctc
     2881 agcggagccg cttttcttcc ttggtaaata ttttttggtt gaagctcttt ccaagcattt
     2941 tccaaggaaa tgcctccttt ttgtttctta attgttttct ttattattct ctttattatt
     3001 ttcttttgct ttatccacga tatcttgcaa ctcttttaag aaaacagaca tatctttgaa
     3061 ctggcgataa acacttgcaa aacgaatata agcgatttcg tctaaatcaa ctaaatcttc
     3121 catcacatat tcaccaatta gattcgtcga tacttcgttt tctccgagac tgcgtacgcg
     3181 gttttcgaca tggttgacga tttgttccat ttgttccata gctacaggtc ttttttctgc
     3241 tgaacggatg agtcctctta aaattttatc gcgattgaac tcttcgcgtt ccccgttttt
     3301 cttgattact aataacggag cagcttcaat tcgttcaaat gtagtgaatc ggaacgaaca
     3361 attctcacat tcgcgacggc gacggatcgc acgtccatca tctgcttgac ggctgtcgat
     3421 tactcgtgaa ttattatgtt gacatctggg acatctcatt ttgtctcacc tcttgcatcg
     3481 cattttatat aaaggatgtg tctttcttta caactagttg tattataaca cgaaacaaat
     3541 cacaaacgcg actgtcctac ccaaaaaaca cggatataag aacaattttt tctactttca
     3601 tgcgtaattg tgtcccctgt ccttactaaa tttcaatttt ccaatactga tcaccttgat
     3661 tactgagaaa taacttacac aaaagctgat ttagtcatct agcacaaaaa acttgaagca
     3721 gatgaacacc ttctcatctg cttcaagtca catctcgatt ttttactatt ttataacttc
     3781 tcttcgattc ctgtatagtg tttcattcgc tgttcctcga ttttttgctc atcgggaatg
     3841 tggtcacttt ctgggagcgg tgttttacta cgatcaggtg tcggattttt tcgttcttga
     3901 gtcaaatctt gccgcttcgc ttcttcttct gcctgctggt cttctatttc atcgattctt
     3961 gcttggtact gcttttcttt ttcttctgct gtttctgatt gcgtacgacc ttcatcgatt
     4021 agattttctt tattgagcat ctcgccgatt ttttcttttg cgttacctac gactttatcc
     4081 ttttgatttt ttaatgatcc catcgctatc cctcactttc tatatactca ggatagcaaa
     4141 attgacacag atactcaaag aaagcgatta gcaaaaagcc aattttcgat ttgtcccaca
     4201 agtttttctt ttgttccatt gttgtcgaac aaaatatccg cacgctgctt tttttcttcg
     4261 attggaagtt gactactaat tctctgctgc gcttgttcta gcgtcagttg attacgtttc
     4321 atcaaacgct gaagctgagt ttctggcgta gtataaacga cagctactgc atccatatag
     4381 tggtcataat gaccttcgta gagcaatggg atatctacaa ttactaaagg atgattggaa
     4441 cttagagagg cgatttgatc ttttatcgct tttcggataa acggatctaa tagctgatca
     4501 agtttttgcc gtttttcctt tgatggaaaa acaatcatcc caagtttccc tcgatctagt
     4561 tgcccatcag gctgcaatac ctcttggcca aacactgata caattttcct cagagcaagc
     4621 gttccaggct caacagtttc tctggcaatc acatcgccat cgatcaaagg aaatccatga
     4681 gactgaaaga ctttggctgc cgtactttta ccggtagcta ttccacccgt caaacctaaa
     4741 acaaatccta ctttgcgatt tttagccttt gacaattcgg acaatagtgc gtcccccttt
     4801 gtgccacttt tgttttcacg atcggcgttc cacaacgtat acatggtttg ccagtttgct
     4861 gatagacatg cagtgctact tgaaatccgc ctgattctcc taatgcattc aaataactgc
     4921 gaatcgtggt acctccggct tctattgcac gatccaaaac atcaatgatc gatagacgta
     4981 attgttctac ctctttgggg cgcagtgtgt tcgccggttg ttctggatgg atcttcgctt
     5041 cccataatgc ttcatctaca taaatattac ctagaccagt cactagacgt tggtctagca
     5101 gcaatggctt gattgctttg tgggactttt tcagtccatt tgcgaaatct gctaataaga
     5161 agctatctgg caaaggctca ggtcccaatt tcatgattcc tcggtaagtt gcacttgctc
     5221 ctttttcgac gattgtcata cgaccaaatt ttcgtacatc gttgtagcga agttggcttc
     5281 catcctcaaa aaagaaaagt acatgcgtat gtttatcgat tggtgtattt tctttcgtaa
     5341 attgatattt tccttccatt cgcaagtgtg agatcagttc acaatgatcg agatgaaaaa
     5401 ttaaaaactt ccctctacgt ccaatggatt ggatggtttc gccaacaaga gaagcctcaa
     5461 agatgggtgt ctctggttgc tcgatgatct tgggccacaa gacctgaact ttttgaatcg
     5521 tttttcctac tactaaccgt tccaatcctt tacgaacggt ttctacttct ggtaattctg
     5581 gcattttttc ttttcacctt cattatattg ctctgtctta aacaatgttt attttgcttc
     5641 gtaccacgtt tttccccaac tgctgtcagt gatcagtggt acatgaagag aaacagcttg
     5701 ttccataact tccttcacca atttatctaa tgattcaagt tctttttccg gtacctcgaa
     5761 gacaagttca tcatgaactt gtaacagcat cgtagcttga agacctgttt cttttaatcg
     5821 tttatccaat tcgatcatcg cgattttcaa aatatcagca gcacttcctt ggattggtgt
     5881 attgatagct gttcgttccg caaaagaacg gatattgtaa tttcttgaat tgatgtctgg
     5941 cagatatcga cggcggtgat acagcgtttc gacgtagcct tggtcttttg cttcgcgtac
     6001 gattctttcc atatattctt tgacacctgg gtatttttca aagtacgtat cgatgtattg
     6061 ctgtgctgct tttcggctga ttcctagatt ttgtgaaagt ccgtaatcag agatgccata
     6121 cacgatacca aaattcactg cttttgcctg cctacgcata tttggtgtga catcctctgc
     6181 cttttcgatt ccaaatacac gcatcgctgt gctggcatgg atatcttgac cttctaggaa
     6241 ggcagctttc aaatgttcat catttgagat atgcgcaagt acgcgtaact cgatttgtga
     6301 atagtcagaa gaataaatca gccagtcttt ttctctagga acaaatgcct gacgtatctt
     6361 tctcccttca tctaatcgaa tcgggatgtt ctgcaaatta ggatcaacag agcttaatcg
     6421 tcctgtttgg gtcaatgtct ggacataacg ggtatgcact tttccatctg attggatcac
     6481 tttcagcagt ccctcgacat aggtagattg aatttttgca atttgacgat aggtgaggat
     6541 atcctctacg attggtgctt gttcacgaag ctgttccagt acatctacgg ctgtggaata
     6601 tccagttttt gtttttttga taacgggtaa tcccattttt tcaaataaga tcactcctaa
     6661 ttgctttggt gaattcaaat tgaattcttc gccggcttct tcgtagattt tttgttcgat
     6721 ctctcttaag cgagcagaga actcgccctt catttcttgt aagcgagtcg catctacttt
     6781 gatcccgctt atttccatct ctgccaaaat gatagacaat ggcagttcca ttttccggaa
     6841 aagatcttct tggttttttt ctgccagttc ttggctcaat tttggtgtca acgcattgat
     6901 tgcagctact tttcgcgcta aatgagcaaa aaagagttcc tcttcttctg gtaaaccttt
     6961 tttcgcccct ttgccgtata cagcttcatc tgattggata tcgttgtatc cgtaatgagc
     7021 agctattcct tcgatatctg tgcttttttc attcgtatcg agaaggtatg cacctagcaa
     7081 gacatcaaaa tcgattcctt tcgtttttcc agtatagcgg tttaaagcaa cataagttcg
     7141 tttcgcatca tatacttttt tcagccgagt cggatcagtg atccaagaat ggaacgctcg
     7201 gttttcgaat acagatagat cgttcgtgac ataaattttc ttttctgtcc cccacgctac
     7261 tccgacgatt tcctctgtat gataattgtc gcccatcatt tccacgtaca acgccatatc
     7321 cgtagtaaac atttcctctt tgaattcatg aacgacttca aacaaaatat catccatttt
     7381 tacttcttct tctacgatat tcaatttaga aaggaactga ttgaaatcca tttctttata
     7441 aaacggaacg agtttatcga gatttttccc ttcatacttc aatgagtcga tagagacatc
     7501 cacaggcgct gaagtattga tggtcgccaa ttttttcgat aagaaagcct gctctttgtc
     7561 atttatcaga ttttctttca ttttgctttg cttcatttca tcgatatgtt cataaattcc
     7621 ttcgactgag ccatattgtt tcaatagctt gatagctgtt ttttcaccga ttttagtcac
     7681 accggggata ttgtcagagg catctccggc tagtcccttc atgtcaataa tctgttttgg
     7741 cgtcaaccca tcatattttt ccgctacatg ttctggtgta tagctttcga tatcgctgac
     7801 accttttaca gtgatatcca cttttacttc tttactagct aattgagtca aatcccgatc
     7861 acctgaaagc acgacgacat caaattgatc tttgtctact ttttctgcca aagtaccgat
     7921 aatatcatct gcttcataat tgggcaattc gtaatactgt acccctagtc ctgtcaacaa
     7981 atcacgtata tatggcattt gttctttgaa ttcgccaggt gttttggatc ttccaccttt
     8041 gtactccgca tacatttctg tccgaaaagt cgtttttcct gcatcgaacg caaccagaac
     8101 atgagtcagt tgctcttttt gcataacatt ttcaaacatc gtattgaatg catagattgc
     8161 atttgtatga agaccgtttc tatttttgaa acgttcaaga gaattgtgaa gcgcaaagaa
     8221 tgcgcgaaaa gccacactgt tcccatcaac gagtaataat ttattttttg tcatcttctt
     8281 aactccttac tgtaaagttc ttattcattc taacaaagaa tcctgcggaa tgcgaaaaga
     8341 aaaagcggga ccataccaaa taattcggta tagccccaac tgatgaattt ttttcttttt
     8401 tagttgttcc gtccaaagat tctcagaaac gccaagaaca agttgataaa gtccaaatac
     8461 agcttcaatg ccatgaatac agcaattcca ctatgtgctg ttccgtttgt ctggacatag
     8521 atattacgga tgctttggtt atcataagct gtaatccctg caaagatgac gactaacagt
     8581 agggagatga acagctcaac tacgctactt ctcaaaatga agatgttcaa gactagtgca
     8641 atgatcactc cgattaaagc tgcctgtcct gctcttccaa ccccgctaag atctcttttt
     8701 gttgttactc cgacaacaga catccccatg aacatcaaag tagctgtcaa gaaagcttgc
     8761 gttactgttc caatatcgta gtatgcaaga gtaaccgcta atgttatgcc attcaaaaga
     8821 gagtaggcaa taaatccacc gattgctaag ctcggattct tttgtgcttt tacgcctaga
     8881 accattacta aaatcagttc aacgatccac agacccacaa agcctagtgg aaaattattg
     8941 ataaaagttg ctaattgcaa aggaaatacc caaagagcca agtaagaagt gactgcacta
     9001 agggcaagtc ccatacctaa aaatccataa acttttgcat aaaaacgatt gatacctgac
     9061 attgatgtac ttggattatt cattcaattc accttcactt tcgattgcta tttccaattc
     9121 aggaacttgt ggcggttgga ccataacgac agcatccaac acacgatctt cgttcgtatt
     9181 ctccgcttct acagaaatcg tcaccacatc cttcatctta tctactttta tgacctcaaa
     9241 ctgaaaagtc agtgtttcat aatggaaaac cggttcaata aaattgacag aaaaattgac
     9301 aacatgtgat cctggtccag gaagatgctt tgaaattgca cttgtgatga tccccatcag
     9361 catcactgat ggaacgattg gctgttgata ttctgttttt tgtgcaaagt catgttggat
     9421 atacaaagga tttgcatcat tcgtcaatcc caaatacagc aaaagctggt tatcttcaat
     9481 agattctgtt aaggataacg agtcgccttc ctcgatttca tttattgttt ttcctagttt
     9541 tctcggctta cctatgttca agatagaaac cctccagttt atttttaatc atacattatt
     9601 gtaccaaaag tagcgtaaaa ggcaagttct gctcccttga tttaacagat tcctatagtt
     9661 tacagaggtc gtgaaaaagc tgtcctgcat caaataataa gaacagccaa gaaaaaacag
     9721 cttctcatgt ttttgatttg attgggctta ttaccgcaga tgcaagcttt tgaacaccgt
     9781 ttatcataag ttgcaaaaaa gggggcttgg caagattttt gccactcccc ctctttctat
     9841 ttttataagg acactgcagt aattgtatta cagtacattt ttcgtcgtgc tgcttaaaag
     9901 tttttcatac gcttgttcaa atttttgaac atctccagca cccatgaaga ttactactgc
     9961 atcatgataa tctaacaatg gagatacatt attttctttg atgacttctc cacctttttt
    10021 gattttcgct ccaaggtctt caatcttaac gtttccttgt tcttcccgag ccgaaccaaa
    10081 aatatcgcat aagtaaactt tatcagctaa atcaagtgct tctgcaaatt catccatcaa
    10141 tgcaatcgta cgcgtaaacg tatggggttg gaaaactgca attatttctt tatctggata
    10201 tttttggcgt gcgccatcaa tcgttgcttt gatctctgca gggtgatgcg cataatcatc
    10261 gactacggtc atgtctgcta cgatcttttc actgaatcga cgttttactc ctgggaaagt
    10321 cagcatttct tctgcaactt ctttcagatc cagtttttcg aaataagcta ctgcaataac
    10381 acctaaagca tttaagatat tgtgtttacc gaatgctggt acagtgaagt gcccaacaaa
    10441 ttcatcccca tgatagacat caaaagcaga accagaagtt gtgcgttcga tgtttcgtgc
    10501 ttggatatca tcgttttctg tgacaccata ataataaatc ggcacatttg cttttaattt
    10561 gcgtaaataa gcatcgtcac catacgcaaa aatagctttt ttcacttgtc cagccattgt
    10621 ttggaaagct gtaaatacat cgtcgatact tgtataataa tccggatgat caaaatcgat
    10681 attggtcatg attgcatagt ctggtgaata ggcaaggaaa tgacggcgat attcacaggc
    10741 ctcaaatgaa aagaattccg cttgcggatc accatgtcct gttccatctc caattagata
    10801 acttgttgga cggacaccac tcaatacatg agagagcagt ccagtcgtac ttgtttttcc
    10861 gtgagagcct gttacagcga tactcgtata attttggata aaatgaccaa taaagtcgtg
    10921 atagcgaatc acttccaaac ctaattcttt tgctcgctgg atttcttcat gtgaatcagg
    10981 aaatgcattt cctgcaatga tcgtcatccc tggttttaca ttatctgcgt taaatggcag
    11041 aatcgtgata tttgcttttt ctaaatctct ttgtgtaaag aaatattttt caatgtctga
    11101 tccttggaca tttagacctt gttcatgtaa aacaagagcc aaggagctca ttcctgaacc
    11161 tttgatacca acaaaatgat acaatttatt ttgattttcc atttgaatcc tcttctctta
    11221 tctagcgtcc attcttttag ctattacatt caacacgctc ctcattatct tataaacgat
    11281 tgaaaatttc aaccaaattt atttggaaag gaggattcgc ttgctaaaat ggtcaagtac
    11341 gagaaaaatc cgtttttatt gactctttaa ttctggtgta agaagaccaa acttcctctg
    11401 atatatcaat gtgaggaata aaatcttttc ctttcggacc atagccgttc tcatcgtcat
    11461 ggagacgttc catttctcct aatactagcg catggaaata ttccttaggc cattgatgat
    11521 tcagttcttt tgctgtaaag atgatctctt cagaaaagtg aaggatttcc ggcacaaatg
    11581 gcacagcatt cgcgaaaata gtttgttcat tttgccatac tttctcaaaa gttgctcgag
    11641 tcctgcgttg cagtgtcgga tcattcatca ataagatttt ttctggtaca gctcctaagg
    11701 aatgaagaat ctccagtgaa aaagtggcgt tctctccaga atttgtagac ttcgattcta
    11761 tcagaaaagc cttatcgggc aaatcataga cttcttttag atactgacga cagatttcac
    11821 tttcactcat gccatcttca aaatgaaatc cttgcttttc aaaattctca taaaggattc
    11881 tagtcgcatg ccccacccct ccgaccaaaa acaattgatc tacttggcca tttaaataca
    11941 tttgtgctgc tttatcagct aagataggaa gacagtttcc agctaaaata agtgtaggtg
    12001 ccgtcagttc ctcaaaattt ccctcttcag gtgcggctaa aaaatttaat agctgattcc
    12061 agtcttttag attcctctgc tcactttttt ccaaaggcct tctatccttt catctgattt
    12121 tttgtgcttc attaaagtct tctctttttg tctcaatcca tttgatttct ccatgacatc
    12181 ttccacaacg atatttgcgt acatcgattc ttctcttgcg gcagatcgtg gcagaacaat
    12241 cttggcatcg ataatactcc atctttatct gtttcttcgt caaggctggt gcatagcgca
    12301 accctcctgt ttttttcaaa agatacttga aatccatatc tttatgctga taccctcttc
    12361 cagctagatg aagatgataa tggcaaagtt catgtttgat gattcctgca atcgtctctt
    12421 cgtctgactc ttcaaatagc ttcgcattaa aatctaaatg atgatcgttt aaatgatagc
    12481 gcccgccagt tgtacgtaag cgtggattga acattgcttg gtgcttgaaa ggaagctgga
    12541 agctttttaa tgatatctct tctactaact gctgcagttc ttcttgtgtc aatttttgtt
    12601 actcctcatt gttactcttc ataaggggaa agcatcgtca aactgattct gccttttttc
    12661 gtatcgactt gttctatcca aactgttacg atatctccta ctgacacgac atctgtagga
    12721 tgcttgacga attttttgct gagtttcgat atatggacaa gtccgtcttg cttcacaccg
    12781 atatcaacaa atgcgccgaa gtcaatgaca ttacggaccg ttccagtcaa ttccatccct
    12841 ggtttcaagt cttccatact caatacatct gtacgtaata agggcgctgg catctcatca
    12901 cgcatatctc gtccaggttg tgtcagccca gctaaaatat cggctaacgt ttcttctccg
    12961 actgataggc tttcagccag tttctctacg gataatcgtg tcaatttttc tactgcttct
    13021 tctgtaccaa gctctttttc tgaaagttgg actgaagtta atatttcttt tgcaatagaa
    13081 taactttcgg gatggatacc tgtgttgtcc aaaatgtttt tcccaccagg gactcgcaag
    13141 aatccgattg cttgttcata tgcttttggt cctaagcgtg gcactttttt caattgagtt
    13201 cgtgcggtga attctccgtt ttcttcacga taaatgacaa tgttttgggc agttgtttta
    13261 ttcaatcctg atatatgctg caagagctgc gggcttgctg tattcacatc cacacctact
    13321 tggttaaccg ctgtttccac aacaaaatcc aattgttcag ccaaacgttt ttgagaaacg
    13381 tcatgttggt actggcctac tccgacagct ttaggatcga ttttgactaa ttcagctaaa
    13441 ggatcttgca ggcgacgggc aatagaaaca gcacttcttt cttcaacttg tagatgcgga
    13501 aattcttttc gtgcaatttc gctagcggag taaacagaag cgcctgcttc gttgacgata
    13561 gcgtaatagg ttttatggtc ggcggatttc aactgctcag ccacaaaaag ttcagattcc
    13621 cgactggctg taccatttcc gatagctacc atatctactt gatatttatt aattaattgg
    13681 ataaaagctg gtccagcagc ttctcttttt gcttgcgctg caggtttatg aggataaata
    13741 acttcgattg ctaaaacttt ccctgtcgca tctacgactg ctagtttaca tccagtacga
    13801 taagccggat caaatcccag gacaacttta ccttttagtg gcggttgcaa caaaagatta
    13861 cggaggtttt cgccgaaaat agcgattgct tgctcatctg ccttttcagt caattcattg
    13921 cgcagttctc gctcgattgc tggctgaata aagcgtttat aactatcctg ataggcttct
    13981 ctgacgaatg aagaagaagg agaggcagga tttttgacta tttggcgatc caagtaagct
    14041 aaaattgctg cttcatctac ttgaaagaat actttcagaa tatcttcttt ttctccacga
    14101 ttagtagcta atattcgatg agaaaccatt ttatggacgg gttctgcaaa gtcataatac
    14161 atttcatata cgcctttttc atctgcttgc tcatccttga cattgctgac atacatacct
    14221 ttgttatacg tataagaccg aatccaagtc cgaaattttg cattatcgct gacttgttca
    14281 gcaataatct catgagcacc gtgcaacgct tcttcggcag aagagacttc tttttctttg
    14341 tcaatatatt tttctgcttc agattgcacc tctccatctg taagctgcat cagccacttc
    14401 gcgagtggtt caagtccttt ttctttagca atcgttgctt ttgtccgtcg tttttgtttg
    14461 tatggacgat acaaatcttc cacttgttgc attttcacgg cttgggtgat ttctgcttct
    14521 aactcgggag tcagtttccc ttgttcatcg attagccgaa tcacttcagt tttacgtttt
    14581 tctaagtttt caagataggc ataccgctct tcgatttcac gaatctggac ttcatcaaga
    14641 cttccagtca tttcttttcg gtaacgcgcg ataaaaggaa cagtgtttcc ttcatttaac
    14701 aaattaagta cagtagtcaa ctgcttagga cgatagtctg aaagttcttt ttgtacaagc
    14761 tgaatgatcg tctggttcaa attttccgtc attaaaatgc ctcttttctg ctaaaaattg
    14821 cttctttatt ctatcatact ttttatcctg ggttaaacta ctgaaagaaa caatattctc
    14881 tataaaaata aaaagagccg caacaaagtc acagctcctt cattctttcc attagtcaga
    14941 ttccatgcca aggtccttcg ttgttctgat attcaatatc ttgaagaata ccttccttgt
    15001 caaaaacaac cccatgcaat gaccctccga atacagcccc accgtccatt ccgattttat
    15061 gatcctgaat ccagaggtct gttgtctgca tatctccatg gagcattggt gtaatcgtat
    15121 gtccaaatac aattgtctta cctgtattgt ttttcccttc atgaaatggt tcccttatcc
    15181 aaataaagtc tcttagactt gtcttacgcc agtctttctt tgtcaaatcc acaccggcat
    15241 ggacaaaaag ataattttcc cattcataat acaaggggcg ctggactaaa aatttaatca
    15301 gttctgaata tctcgattgg atcatcatcg agatttcagt tggggagtat tcctccgatg
    15361 cccctgggtg aatcaggctt tcaaacgttt cttttccacc atttctcata taaggcgcat
    15421 accagtcctc cggtgattgc agaaattgca aaaaatattc ttcatggttt ccccggaggt
    15481 aaatggctcc tgtttcttcg actaattgct ttcctaataa aaaacattct tttgtacgag
    15541 gacctcgatc attgagatcc cctaataaaa caagctgatg gatctttgga tcataatagt
    15601 gaataagttt ttcaaaaagc tcatgtttgc catggacatc cccaatagca taaatcaatg
    15661 atttcatgtg gatcaccatc ctcaacgcta gtatagcgca tattggaaat ggtcgacaat
    15721 caaaactatt gttttctatt taattaaagc tgtaaaccgc agttctgctt cttctggaga
    15781 gattccatgg tcttgaacat agcgattgcg cggatgtttc gcacactcta tgctacatgc
    15841 tcctaaatgt ttttcttcat tttcaatgga agcaagaatc tgacgattgc actcaggatt
    15901 gctacaattg atatatcgtt cacatggtgt tccgtcaaac cagtcgcgac cgacgatttg
    15961 tttatctact tggttgatat cgacagcgat ccgttcatca aacacataca tcttgccgtc
    16021 ccataattct ccttggactt caggatcctt accataagta gcaataccgc catgcagctg
    16081 tccgacatct ttgaatcctt cacggacaag ccaaccagaa aacttctcgc aacgaatgcc
    16141 accagtacag taggtcacta cccgtttttc cataaattct tctttgtgct cgcggatcca
    16201 gtctggaagc tctcgaaaac tgcgtatatc tgggcgtaca gcccctcgaa aatgtcccaa
    16261 gtcatattca taatcattcc gtgcatcaat caccactgtc tgttcatcta aaatagcttc
    16321 tttaaattct tgcggtgaaa gataggcacc tgtcacttgg tttggatcaa tgtcctcttc
    16381 tagctttaaa cttacaagct ctggacggta tcttacaaac atcttcttaa aggcatgtcc
    16441 ttcgcttgga tcgatcttga acatcgtatc agcaaatcga ggatcagctt tcattgcttt
    16501 catataggca gtcgtgtttt caacggttcc agacacagta ccatttattc cttcttcagc
    16561 tactagaata cgacctttaa gatccaaaga tttgcaaaag gctaaatgat ttttggcaaa
    16621 ttcctctggt tcttcgattt ttgtataatt gtaataaagc agtacttgat aatccatgta
    16681 tttcttcctt ccttcttttc gttcctaaac agtataaaga aggcgttcgc atttgacgat
    16741 ttatttgctt gtgtataatt acgcatggaa agttttttgt ttacataata ttattatttg
    16801 aattaaaaag acttcgcttt cctgtttcgc acagaaaaac aaagtcttta tttctattat
    16861 ttacctgtaa ccgcaaagta gttgccatcg ggatcagcaa agttaaagac atattcttct
    16921 ccaagctgta ccatatcacc taatgtgact gattgcgtct gcattttttt atatagtcca
    16981 aagatatctt tactgtaaaa catcaaagat ggggcactag tagctgtgcc aggtgaatgg
    17041 gactcaacaa atgctcgatc atataacaga aatgaggttt gactgtcctc aactcctact
    17101 tcaattataa gcgtcccatc catttcttga cgatcttttt ccgcaaagcc aactgctctc
    17161 caaaacgttg catttttttc tacatcgtcc acataaagca tgatttttat ttcattcgta
    17221 aacatctgtt tcctcctaaa cgatctgttc taacattctc aactttgtca gtttaccata
    17281 ggaaaatggg aaaatctacg aaagctctca acagaatcgt cctacttaaa tacgcttttc
    17341 tttaataata aataagttgg ttgtttttgt tatttgcgct ttcacttttc ggaataaata
    17401 atgtgtattt atatggaata aataaaaaaa gaggtcgtga ctaaaaacaa gtcacaacct
    17461 ctggtaaacg gtgttctcct gacaactcct cggtcacaag acacaatatt caacaaacat
    17521 gagaagctat tttttgaaat tgctctttgt gactcagagt gaaacggcag gctcacgacc
    17581 tctttcttaa acggtgttca aaagcttgca tctgcggtaa taagctcaac caaacgtaaa
    17641 atatgaggaa ctatttttgt ttggctgttc ttattacttg atgcagggca gctttttcac
    17701 aacctcttat tcacggtgtt tcagaagtag cttctcggaa ataagccgaa atttcacaaa
    17761 aatttgagaa actattttta ggaaacttct tcttatttct taaagctgac cacttctatc
    17821 acaacctctt tttgactatg cattcacatc gatcacttta gaatcatcat tgatgacatc
    17881 gcgatctaaa tctttgatgc gtgcacttgt cacgatacca gctaccatgc taccgctgac
    17941 gtttactgct gtacgtgcca tatcgattaa tggttcaaca gagataacta acccaacgat
    18001 tgctactggc aagttcattg cccctaaaac gatcagtgaa gcaaatgttg cgcccccacc
    18061 gactccagct acaccaaaag aactgattgt tacaattgcc acaagcatca aaataaattc
    18121 caaactaaag acattcactc cagctgctgg tgcgacgatc gttgccaaca tagctggata
    18181 aacgccagca cagccgtttt gtccaataga caaaccaaaa cttgctgaga aattagctgt
    18241 tgcatcatct actcctaaag cttttgtctg agtctcgata ttcaatggca aagctccagc
    18301 acttgaacgt gaagtaaacg caaagctcaa taccggaaag gcttttttga aatagttgat
    18361 tggattgact tttgcaccaa tcaaaatcag tgagtggaca atcaaaacag caagcaatgc
    18421 tacgtaagaa gcaacgacaa atttacctaa attgacgata gctgcaaaat cacttgttgc
    18481 caaaacgttt gtcatcaaag caaatactcc ataaggtgtc aaacgcaaaa caagcgtcac
    18541 gatccgcata atgattttat acagactgtc gactaaatct gaaaagaatg ctgcttcttt
    18601 tggcgctttt cttttcacgc ctagataagc gattccgaca aatgccgcga aaataacgac
    18661 tccaatcgta cttgttgcac gactacctgc taaatcagca aaaacgtttt tagggataaa
    18721 ggataaaatc tgctgtggaa tcgataagtt ttcaactgtt tcctgacgtg ttgccagttc
    18781 agagattcgt gctgtttctg cagctccttg tgtgaattta gctccttgga gtccgaacag
    18841 cattgttgaa ccaattccga tcaatgctgc aatagctgtt gttccaagta aagaagtcaa
    18901 tacagtcgca ctaattttcc ctagcttttc agaaactttc atttttgtga atgcaccaat
    18961 gatcgaaata aacactaaag gcataactag catttgcaag aatgatacat atccttgacc
    19021 tacaatcccg atccattcca ttgattgagt aacgactttg tcgtctacac caaagatcaa
    19081 ttgaagcaga ccgccaaaga cgataccaac tcctaatgct gtaaagacgc gtgttgaaaa
    19141 ttttgtatgg cgcttttgca tacgatagaa cacatataat aatgccgcaa aaactagaac
    19201 cactaaaact gtgatgagtg ttgtcatttt tctcctcctc atattattaa tgacaagtcc
    19261 atcgaaggaa gtaaaggttc ccgagaatga ttcttcaacc atcctcctga aaaaattttt
    19321 ttcagtgact tttatatttt agacgatttt tatcgaaatg acattctttt tgcttacaat
    19381 tagtaataaa aaaacatacc tgtgatagct ttaatctatc gcaggtatgt ttttactgtc
    19441 tattcttgtt tttctgattg aaaattcaat tttaccccag gctgatctaa ggcgatctct
    19501 gtcacctcat aagacattcg gcgtacgata tcgaataacg gtgctactag ctcgtccact
    19561 tcttcttgtg tgacatcatt ttgaccttca attttccgat tgaggatatg attgatctga
    19621 ctcacacttc cagataaaac atatgtatca aacactaaac ggaactctaa gcgtgctcct
    19681 acgatgctat gttctttcgg atagttttcg atcgtttctt ttaaaggcga gaagcctact
    19741 ttcaaatctg tttctacttc ttggttcggc aatctcatat cataatgaaa tgcttctaca
    19801 acttcttttt gtcttctgat ttccatataa ttttatcact cctttatgaa aattataaca
    19861 tatctccttt gaaaggggat actacttttt aatgatgctc tgcaaggtaa ggatttcatt
    19921 cagatttcct accacataat ctgccgggat ctcacccaga aaatgatcga tgtcgtaaag
    19981 acaagtagcg actcctgcat ttttccctgc ttcgatatct aaacgacgat cgccgatcat
    20041 catcgtgtct gttctttcta aatgaaaagt atcgatcaaa taatttaaag atgctggatc
    20101 aggtttacga ggaaagtcct gatcgatccc tactacttcc tcaatcagat gtgccaatcg
    20161 atgttctttt aacaggcccc atgttgattc tgtcaaacgg tgggtcagga tgaaatgacg
    20221 accgccgttg tcttttagca tttccagtac ttgtttcgtt tctggaaatg gacgggaaac
    20281 ttccgtactt tctttttcat atcggtgaaa taattcatta aactctctgg cattcaagtt
    20341 tgattcattg atcaaataac tagtagaata ctttttcatt ttgaagtaga tttctttttt
    20401 gtccatactg ataccaaaat cttttagtgc ttgccatgca ccatctacca ttgcgggata
    20461 cgtatcaaac aatgtaccat caaagtccca tatataattt ttcatgttcc ttttgttcca
    20521 ttcctttgct tcttttttct atctctcact tttagataaa ggtgttcttt aaagtatttt
    20581 tttgtccaga attgtcaaat catctgctaa tgtatactgg attggttgtg catttgggat
    20641 atcgatcgta tccatttgat cttcaggaac atcttctaaa tattttgtca gtgctcggat
    20701 gctattacca tgacccacaa ctagtaaatc ttgccgatct tttagttttg gtgctagatg
    20761 atcttgccat aagggaatga tccgattcac tgtcatttcc agactttctc cgtatgggat
    20821 caaatgcgga tccaagtgat catatctgcg gtcaaatcta ttttctgcgg ttaacggcgg
    20881 aacttccgta tatcctctgc gccatgtttt tacttgatct tttccgtata tttctttcat
    20941 cttatctttg tttttaccca caagatcccc ataatgccgt tcatttagac gccatgtttt
    21001 tattatagga agccataact gacccatttc ttctagtgcg acttgtccgg taagaatcgc
    21061 acgtttcaaa acagaggtat aaacggcatc gaattgaagt ccggcttcaa gcattttttg
    21121 accggcaact gctgcttgtt cttttccttt ttctgttaag gcgacatcta gccatcctgt
    21181 ccagtaattt tcaaaatttg cttcgctttc gccatgacgt aataatacta atttcattct
    21241 tttctccctt aaacattcgt ttcgaatttt ataccacatg ttttgcatgt cacacgtatt
    21301 cgtccttttc ctttaggtgc acgctgcttt tgatgacaat tcgggcactc aaaaaaagta
    21361 taactttttc gatccatttt gacttgcttt ttgtttttta tctgatgata tttttctttg
    21421 actctatcaa gatatttcaa ataacgttga ttctcattta gtctggggta gatttttttt
    21481 gataggaaac gaaatatcat ccatccgaaa aagacgatga acaacaattg tccgattcta
    21541 tagggcaacc aacgacttag caaactaaag acaagcgcag cgatcaatag accgcgattc
    21601 aactgatcaa atttcgcata tcttccctgc ataagtttgt gatagttctg ctgaaatcgt
    21661 attagccttt ctaaccatgt ttgcatcata ttatctcctc catcatctaa tatttattat
    21721 gacaaaaaaa tccaaaaaga aaaagcctta attggccttt tccattgcaa cttggtgaag
    21781 gacattccga tttctggtaa agtcaatcac aatcactttc tttttatctt ctctgttgac
    21841 tactcgaaat gtgtccgtca atggaagaaa cagcttctta gatggtagct gttgcatacg
    21901 tataaaacga tggatcatcg taatcaattg aggaaattga atagacgtgt gaagcagacc
    21961 tggatatttt ttcaacagct gtgtcgtttt gatcagattc cgttttttac tgatcacttt
    22021 taaaaagctt tttagaatct tttgctgacg tctaaaaata gaaaaagaat tttcgtcatc
    22081 tatttgataa cgcatttgtt tttgcagttc tgctgcagtc agatggatct tcccatcgtt
    22141 tgtatcgatc attaaacctt ctggaaacag ttcaaccact gctcgatccc aagattcaag
    22201 agaaagctcg atataatctt ttactggtaa ttccaattct accttaaaaa agtgcaaaac
    22261 agccgacagc ccttctgctt gatagatttc tcctaatgtt agcccctcta gatggaactc
    22321 tggtgaaaaa tgcagcaatg ttttattttc ctgcaattga acatatagaa atgtaaatcg
    22381 ctcaatcaga gtatctttta aaacaactaa aaaattcatt tttctccccc ttttttcatc
    22441 atttttaaaa tagcatattt tgaaaagaaa gtctgatgct tagagtagaa tttcataaaa
    22501 atttactatt tcataagtat tcacgagaaa aatacaaaca aaaaagcgga aataacgctc
    22561 atccgccttc ttgtttgtgt ttatttcctc ttcaaacgct tgtctatgat tcagactttt
    22621 ctctatcgtt attttcatct aaaagttctt gcattccgat aatttctcga tattgatgaa
    22681 tcttagtgat accaatgact ttgtctacat caatactaaa acaaatacca ttacctggaa
    22741 tatcgatgtt gatgccttga ccaatcgcat ccattacttt attcgtcaaa ctatcgggta
    22801 ctaatgtcaa cacaatatct ttttctggcg aaatgtccaa acctaaaaat ttccctgaat
    22861 catgtacacc agaacctctt ccatgtaaga tcgttccgcc tgctgcacct gcttcttttg
    22921 aaaagtcgat aacatcttcg cccattcctc cgtcaacgat cgtcacaatc atgtgaagat
    22981 tcaatgctac aagatctttc atttgctttc ccccaatttt tgcaatcttt ctttttcgat
    23041 tctgtctttt tcatcttttc gtttataaat atatcctagt actaaagtgg ataatattgg
    23101 tgtcaatgca atcattgcaa ccacaccaaa cccgtcaatg atcggatctc ttccttcaat
    23161 ttgtgcagcg attgccacgc tcattgataa aataaatgtc gaacacatcg ggcccgttgc
    23221 tactccaccg ttatcaaaag ccattgctaa aaagatcttg tccacttttc gtcctaaaat
    23281 aaatgctagc ccgtatcctg ggatcaagaa ataataaaga gagaatccga cgagcaaacg
    23341 ccacatagac aatcctactg ctgcgccgat cccaattgaa attaccatca gcatgatttt
    23401 cgatcggatt ctcccctcgc tcaattcttc aacctgtttg atcattacat gaatcgccgg
    23461 ctcagcaaac cctactaaga accccattaa aaagccaagg ggaaccagta tataattgta
    23521 aggcaatccc gcaatagcac tgcctaaatg ctgtcctacc ggtacataag ccatattgac
    23581 tccgtgcagg aaaaggatca acccaatagt cgttatcaag aaacctttca taatgtcaaa
    23641 gaaacgcttt ttgcgcatac gaaaactaat gatattcatt aaaacaaata ggattacgat
    23701 aggtgccact gccaaaatga cttccatggt aacgatttcg attccatcta tcacttgctg
    23761 ccagaaactc attggaagat cacccccaaa atcatcacgg caagaatcgg ccctaacgaa
    23821 gcaataccga ccattccaaa actgtcattt tcatttttct tttgtttgat catactagtc
    23881 atccctccgg caagggccaa gataaatgga acagttactg gaccagtagt cactcccccc
    23941 gaatcaaaag caatcggcat aaattcggaa ctggtgaaaa atgaggcgat caaaacgcct
    24001 atgtagccta tagccatcaa ttgataatag gatagcttga atacgacccg aagcagcgca
    24061 aaggctagaa atactccagt gccgacgctg acgatcccaa tcagcaacac tctgccaatc
    24121 tctccgtcag atatatcata tacttgttgt cccaaaactt gcacggaagg ttcagcaatc
    24181 gtgatcacga tcccgatagc aaatcccaaa ctgaccaaaa cggtcaaact tttctttttg
    24241 atcatatatt tccctactag atctccgact tccatcatag aataatctgc tccaaacaaa
    24301 aagagcgtca ttccgatcat catgattgcc gcaccaacca gaaatgaagc gaggtcttct
    24361 ccttctaacg gtgcaaaaac aaacgttaaa atgacaatca ggacagtcat cggcaatata
    24421 gcagcaatca cttctttaaa attttttagc atgtatcctc ctcctttact ctttaagttt
    24481 accttaaaat gaaagagttt tcgagcacaa acttaaaacg ttatcattta atttttctca
    24541 acataaaata acacacgtcc caaaagtatc tttttacttg gaaaaatgac tttgaaggaa
    24601 cgcgcttatt gtgttttttt attttacaga gccgatcatt ccttgaacga attgcttttg
    24661 caaaacaaaa aatacgatgg cagttggtaa agtagcgatg actaatccag ccatgatcat
    24721 tccatagtct ggtgtatagg aagcacccat agcagataaa acaagcggaa ccgtccgttt
    24781 atcaggagat tgcaaagcta ctaagggcca taaataattg ttccagctac tcatgaatgt
    24841 gatgattgca gccgcggcat aggtattttt agccgtcggc atatacactc taaaaaaaat
    24901 acttagttct tttagaccgt ctaatcgagc ggcttctact agatcttttg gaaaagattt
    24961 aacattttgt cgaaagaaga agatcaaaaa agcagtgcta acagatggca ggatgacaac
    25021 caagaaactg ttgattccca aaaccgttcc gttcaattgt gaaaacattc gatataaagg
    25081 gatcatcaat gccgcaaaag gaaccatcat cgatagcagt agaatattga aaattaggtc
    25141 ttttcgttta ctttgataaa tctcaaatcc atatcctgcc attgacgaaa tcagcaatgc
    25201 aaacacagtg gtcaccaaag cgatcatcgc tgaattccaa agggaacgag taaaattcag
    25261 ctcattggaa aacaaatttt gaaaattgat gatcaagtta ctgccaatct ttatttttcc
    25321 cgatgtgata tccacgggtg tattcgtcat tcccaaaacc atccagacaa aagggaaaat
    25381 ggaaataaat gataggatgc ttaatgctgt atactttaat actgacccaa ttgtcgaacg
    25441 aagttctaat gcttgttttt tgacgttggg gcgttcgatt gtttcttttt ctaccatatt
    25501 tttcatcgac tttctcctcc tactttcgtt tggattatcg acaatatcac aataaaggaa
    25561 acaataacaa aggaaactgc tgccgcatat ccaaaattag gcgtgaattt ataactcaaa
    25621 ttatagatgt attgagatag ggtggtcgtt gcatttgctg gtcccccgcc agtaatattt
    25681 tgtacttcat caaaaagctg caatgtcccg atcgtcgaag taatggaagt aaacaaaatg
    25741 attggtttta aatttgggat cgtcactttg ataaactgct gccatttaga agcaccgtca
    25801 atagatgctg cttcataaat ctccgaatca atattttgca tgcctgaaag gaaaaaaatc
    25861 atattgtatc cagtccaacg ccaagtgatc gaaagaatga tcagaactct cgcccaaatt
    25921 ggatgagtca accagccaat cggtgtgtca atcataccaa tgcccaacaa caagttgttt
    25981 acaaggccag attgagaaaa tagacttttc atgatcagag aataactgac catcgaagtg
    26041 atacatggaa gaaagatagc ggttcgaaac aaccctttat atttcaactt tttatcattt
    26101 aaaaggttgg aaataaaaag tgctagaaac aacatgatcg gtacttgtac aagtaaatac
    26161 aggaatgtat taaaaaaagc ttttttaaaa gttgcatctg tcaacatccg ctgataatta
    26221 cctaacccgt taaatgtcat attgacaggg ggaccacttt gaaaagacgt gatcaaagcc
    26281 aaaaccatcg gaataaaaac catgattgat atcaaaatga ctggcaagat taaaaaagca
    26341 tgcccgtttt tgaataaatc tttctgcttc attcttttcc ctccaattct atcattgatt
    26401 atctacatga aaagacaaga aagacaatat ctgtaaggca gacgtgatct tttttgtctt
    26461 ttcgtttgtc tgatgaaaaa gcgaagaaat tcactttttt accaccgatc aattagccaa
    26521 ttgtgcttct acttgttttt gtgtatctgc tagtaccttg tctgtctctt ctccattgat
    26581 gattcgatga agagcttcag ctacgactgc ttcaattgca taggtctcat gtccgtaatt
    26641 cacttctggt atttcagatg tccatgttga aaaatcgtca aagatctttt gaccactgta
    26701 aaattcagaa gcttcttgat aagcttcttg ttcattcgcg ctaagcatcg tagatactag
    26761 tccgatttct ttcgcaagag tgcccatcag ttcttcattc gaagcaaacg ttgcttctag
    26821 gaaatctttc gctgaatctt ctccagctac tcctttgatc acataccagc cagcgccgcc
    26881 aaggttagaa gcttgcgctt tttgcagatt ttctggaagt gcaggtattg gtgcaatctt
    26941 ccattttcca gattggtttt ctgctccttg gatcgttgaa gaataccaag cgccagttgg
    27001 gctagaagct actgctcctg attgtatcgc agttactcca gcattccagt cagaggtttg
    27061 ttcgactaga tcttctttca aaagagtggc aaacaattca agaccatact ttaaagatgc
    27121 attgtcttgg atcgtgactg tctccccatc ttcagatgta taccattctc ctgcctgttg
    27181 cataatcatc cttactcgac caaggtcaga aggattgact tcagcgattt tctttcccgt
    27241 cttttctttg acatctcgcg ctacttgaat atactcgtcc cacttcagat tctccatatc
    27301 ctcttctgta taacctgctt ctgccatcag atccgtacga tagaagtttg ctgttacgcc
    27361 cgaatcgaaa ggaacaccgt atattttatc tcctactttg tttaccgcaa atttatacgg
    27421 tgcaaaatga tcttccttta caatagcaga caaattagcg aatgcatctg gataagaagt
    27481 caaatagcct tgtatcctat aatcttcaat caagacgata ttgggcaatc cttctgtatt
    27541 cccgctcgct aaagcggtgt tcagtttctg aacgatatca tcctgagaca tcgtaacaac
    27601 ttctatctct gtttcttcgt tctcataaac tttttttgct tcattcactg ctttgatatt
    27661 gaatgtctca tcccacgccc agacagtgac tttttcttct ttgtctgctg tactactagc
    27721 acttccgcat cctgttaaga aacctgatgc tgccgctaaa caaacgactc cagcaattaa
    27781 ctttctgatg ttcattttta tccgctcctt ttccatccat tagttgttta cgtttgctac
    27841 attcatcata ccaaaatttg aaaacggttt ctttattatt cagatacttc aaaaatttct
    27901 cctaaaataa taaaaaaata caaatcaact atgacaaaag gaataatctt agcatctaga
    27961 ccaaagatcg aaatggtgag gtttctttaa aataaaaata tccttgcatt ttctcctaga
    28021 aataagtatt tttaaattac aagactattc ttaaggcggg taaaaaaatg ccaatacgcg
    28081 taattcttat cgatgatgaa cagacgattt tagatggaat gaaacagttg atcaattggt
    28141 ctgagcacgg gtttcaatta atagggactt tttccagtcc gctgaaagcg cttgattttt
    28201 ctttggtaca tgcaatcgat atcgtggtca cagatgtaat gatgcctgaa ttaaatggaa
    28261 tcgaattatc gcggctatta aaacagcaac aacctgaaac acagatcctt atcctctcta
    28321 gctatgatga atttgatatg gttaaagatt cttttaaaga aggcgtcgct gattatcttt
    28381 taaagcctcg attgagtcct gatttttttc taaattcctt aactgccgtt tcacaaaaaa
    28441 tcaaaaaaaa gcccaacaaa agcccaatca ccaatcgtta tgagcagatc tgcgagaatc
    28501 tttcacagca gattgctgga caaactcgtc cattgcttga ccctcttctt tttgaacaca
    28561 ccttgttttt ctgttatata ccgaaaaacg gccattcagt tcaaaacaag gctctaaaca
    28621 aaaacagtcg atcacccatt cattgatgga tagcgacgat tcgtttacta tttatcctgt
    28681 ttctaccttt tcggaagaat ccggctatgt aatcaatact gattccaaag atacacttag
    28741 gaagtttgtt caaaagtttc gtcaagaagt ttttccaggt gatttttttg ttgtttctga
    28801 ttcttttgca atccaagata tcaatgaaat attccaagag atcagaaatt gcaccaaagg
    28861 acaacgtttc tatcataagg gaaagaacct tgcttatcag catgaacttc ttccgttatt
    28921 aaccaaccac gagcagcaag caagtagtta tttaacaaaa atcgttcata aagactacgc
    28981 tttagctgtc aaagaacttg agaatttctt cacgacgata ttggaacagc ctattgatcc
    29041 agcttatttg aaacatgagg caatcaatcg tttctatgca ttactcaacg ctttggctga
    29101 cgagcatgtt gggaatgaag aactttcttt gctcaagatc gcccttcctt ctttattagc
    29161 cgatgctgag tatttcgaag atttttctat tcttgtcaaa gaaacacttg agaaactcac
    29221 ccacttttct gcaaatgcaa aaaaagacaa tttcgatatc ttgactttga tctacgagtt
    29281 cgtccaggaa aattatgaga aagaaatcaa tttacaactt ttatccgaaa aatatcattt
    29341 ttcatatagc tatctctcag ccatctttac tgaaaaattt ggtatcaact tttcaaaata
    29401 tctaaaaaaa gtccgtatca gtaaagccaa gctctttttg acggaaacac ggcttacttt
    29461 gacggaaatc tgtgagaaag tcggctatac cgaactgggt tatttctcca gagtttttaa
    29521 agaagagaca ggaatgacac cttctcaata ccgtaaagga acttttgtaa aatgagaaaa
    29581 aaaatcaaag agcatgaact ctttgccaaa cttctgttca ttttgttcat cggtcttttt
    29641 ctgcaagcca tcgtcatttc tttgtttatt tatcatcgat caagggatgc ctatatccag
    29701 ctgttcaatc aatcgaatga tgtggtacta aaaaagattc aatctgaatt tgaaagtttg
    29761 aatgatacaa tcgagaatac attagccgct tttgattcta atccagctgt caaaagctat
    29821 ttttccaatg atccagccca acatatggaa cagcttcagc aactaagaac aatccaaaaa
    29881 atgaatgact ctctttcaaa aatccatcca atgatcgatt acgatgtatt gatctttgga
    29941 gaaaatggaa gaacttttgt cggaaacgac atgctcactg ccgtatctgc cgattctttt
    30001 tttcaatctg cgattgctca gagagtgaat gaacgtgctg ctgatacaca aatgcttttt
    30061 gcacaccacg gattgactct tcgcgataag aaagcaccca gcgtcttctt tgtcaaaaaa
    30121 ctgaaaaata cattgaatca cgtttacgga tatgctgttt tatcgatctc atccaagcaa
    30181 ctagcgaatc tttttcaatc tgtagtaaat cctgagatat cgaagatctc cttcatgaac
    30241 gaagaacaga cgatcatcgc atcaaatgaa gaaaatacaa ttgggagccg atcaatgatt
    30301 ttcaaaaaac taacagtagg tgaaacaacg atttcaaatg gacagcagtt gacccgtctt
    30361 cctctgtacc gccaggattg tacattactc agtcaagtcg atcttcattc tttatctaac
    30421 caaatgggcg tgatttttcc gattattttg tataatatcg tgacacttgc ttttgctggc
    30481 tcattggtat ttatgtattt ggatcgacac acaaaatcga tctaccgctt gattgattcg
    30541 ctaaaacggc tagaggaaga tggcttaaaa gctactgttc ctgtacaagg aagccatgaa
    30601 gtccaattgc tagggaatac gatcaatcaa ttgctaagta atgctcagca tcattatgaa
    30661 cagacgatcc aagacgaaaa gaagaaacgt tcgcttgaaa tccaaactat gcaagcacag
    30721 atacagcccc attttattta taatacgctg actagcttga aatttttgat ttggcagaaa
    30781 aaaacagatg aagcagttca aggaatcgac agtttcatta ctttactaag aagtacgatt
    30841 gggaaaaaag aagaagtgat tccagtcaag gaagaaatca aaagcgtaca aagttacatc
    30901 gatcttttga gtatgcgata tggcgaaaga atctcggcta aaataatgat tcctgatgaa
    30961 ctgcagtcat tatctatgcc gaatatgatc cttcaaccaa ttatcgaaaa cgcctatctt
    31021 catgcttttc acaataaaac gcaaggattc atcacggtgt acggcaaaac aaaaaatcag
    31081 cttcttttat tcgaagtgat tgataacggc gatggcttcg atactgaaaa taccaaacaa
    31141 aaaaatgatc atttttcagg aatcggcatc agccatgtag atgagcggat acgcctaatg
    31201 tatggcgatg catatggctt aacgatccaa tcagtgattg ggacaggaac gactgtaacg
    31261 atttgtctac cgcttcttcc taaaaatcaa tcagaatatc aaaaataaag aatagccaag
    31321 cgctgatttt tttattttaa gttaaataaa tcaatcagta ttcttgactt tcatctacgc
    31381 gtctcttcta ctgtcagcac tggataacag cccaatattt gactttctcc tccgagcatc
    31441 ttgatttctt ggagcgcatt ctctactaat gtcattggac ggtccaacaa caaatcgatc
    31501 acgaaaaaat attctcccaa gctagttttc aatggtcggg attcaatctt acttaagttg
    31561 atttcccgcc agccaaaagc tgccagcatt ttatgaagca tccctggccg attagcaggt
    31621 agtgtcaaga tgacactcat ttttactgga gctgactgct ggctggtcat ctttcggtca
    31681 ccgacgatcc aaaaacgtgt ctggttcaac tcattatctt ggatattctc agctaagatc
    31741 tccaatccaa catgctgtgc tgtttctaat gaagcaatcg ctgctgcgtc ttctttcgga
    31801 tgttctgcta catacattgc tgcagcagtt gttgattctg ttgctactaa aggaacatta
    31861 ggataatgcg tttctaaaaa ctgttgtgat tgtgctagtg cttgtggatg tgaaaggatt
    31921 tttgtaatct ttgttgctgg attgcctaaa agctgctgct tgataggaag aacgatttca
    31981 cttttaactt cgacttctgg atgtttggaa agaagatcga ttgtgtgatg aacagctcct
    32041 tccaatgaat tctctacagg aacgacagcc agatcgactt gtttgcgaaa caaagcgcgc
    32101 aaacaaacag ggatggaagc atacgcagtc aattgttctg taggaaatag ctgacaagcc
    32161 gcctgataag tgaatgaact ttctggccct agatagctga cgttcatatg cttttgatcc
    32221 tttctataat ggtatcgatg atcttttctg gtgtttgatt ggtcgtgttg attcgatgtg
    32281 tcgcactttc ttcataccaa gaaagtcgtt gtgccaataa tgcttttatt tcttctcttg
    32341 tttttccaag agctaaaggt cgaatagacg tttcatcttg ttggatacga tcaactagac
    32401 agtccgcttc tgcctctaag taaagaacaa tcggctgtgt ttttaaaaaa cgccgatttt
    32461 ctgcactttg gacaatccct ccaccagtcg cgataatttt gtcttcattt gacagctttc
    32521 ttaaaatatc tgactccact tttcgaaagc ttttttcacc gtaatagtga aaatattcgg
    32581 caatcgacaa ttggatatgt tcctcaatct ttgtatctaa atctacatac tctttttgta
    32641 aacattgagc taacccttta ccgattgttg tttttccagc gcccatgaaa ccaatcagca
    32701 aaatgctagc catgagcttc tcctcctgac acaagttctg ctaaatcgtc aaaaaatgcc
    32761 ggataagaga ttgaaacagc ttcagctcct tctagttcgc aaggtccatc agctatcaaa
    32821 gcagctactt gcagcatcat accgattcga tgatcgcctc tgctggacac ttttgcgcca
    32881 tgcagaggtg tcggcccatg gatgatcaga ccgtctgctg tcgcttttat ctttgctccc
    32941 attttctgta attcttctgc caccgcatca attctgttgg tttcttttac ttttaattct
    33001 tctgcgtctt tgatgatcgt gattccttct gcttgagtag ccactaaagc tagaatgggc
    33061 aattcatcaa tcagacgcgg aatgatttct ccaccaatcg ttgctttctt caaatgactc
    33121 gtcttgacac ttaaatcagc cgattggttc tgttcattgt gattcgtctg ggtaatcgct
    33181 gcgcccatct cctccaacac atctaagata ccagttcttg ttggattgac cccgacattt
    33241 tttaacaaca gttggctttc cggaacaaga agtcctgctg ctagaaagaa agcagctgat
    33301 gaaatatcac ctggaacagt tatctgttga ccagttaatt tttgcggtcc ggtcactatg
    33361 atcgttttat cttccactgt gatccttccg ccaaattgac gaatcatttc ttccgtatgg
    33421 ttacgggaac gttctttttc aacgattacg gatgtgcctt ctgcttgtaa tgccgcaaac
    33481 agcaaagcag atttgacttg cgcactggct acaggcatat tgtatcggat aggtgataaa
    33541 gagcgggttc ctgtcacagt gattggtaga tactggtcat tttcttctcc ttgcaagtca
    33601 gcgcccatct cgcgtaaagg ttccatcact cgccccattg gacgtttaga caacgaagca
    33661 tccccgaaca gagtagtcgt aaaaggctgt ccagccaaaa tacccatcaa taaacgtatg
    33721 gtcgtaccgg agtttcccat gtcaattgat tcggttggtt ggacaaatga gtggctaccg
    33781 cgaccatgaa tcttgatcac ctcttcctct tcttcgatct cgacacctaa ttgttggaag
    33841 gcatgtaaag tactcagaca atcttctgcg cgcaaaaaat tttgtatagt cgtcgttcct
    33901 tcagcaattg ctccaaacat gatgctgcga tgggaaattg atttatctgc tggtatccta
    33961 acagtccctc ttaatccatg tatttgttgt aataattgca tttatccagc ctcctatttg
    34021 atccagcaat gataactggt attagacata atgattcttt ttgcttgtaa caaatcctgt
    34081 tggtttttaa aactgatttg cagtattcct aaaatatctt ccctggtctc ttggatcttc
    34141 aaattaatca aagaaactcc cgcttttccg atcagtccag ttacttctgc aataacgcca
    34201 ggttcatctg gcacatcgat aaataaatca aaaaaggcag gaatcgcgcc ttgcttatgg
    34261 acaggcaact ggtctcgact ttcttttgcc cgattgaaaa aagaaaagat ttccgattgg
    34321 ttctcagtca tgatccattc tgctatttgt gtcatttctt gttgccaatc tgccaatcgt
    34381 tccaataata tctgcttatt gctcaacaaa atatctgtcc acatgacagg atctgaggaa
    34441 gcgatcctag tgatatctcg aaagcctcct gctgccagtt gtttggctcg cggatactct
    34501 tgattgaatt gttctgcctg attgactaag ccagctgcca gaatatgtgg aaaatgactt
    34561 agcattcctg ttatacggtc atgttctctt gctgtaagag ttacatattt tgctttggtt
    34621 ccgcaaaata gttcttgcag tttttcaaca tcttttttcg tcttttccaa aggtgtaaag
    34681 atgtaatagg cattttcaaa tagtttagca tctgctgcct gtatacctga tttatgagaa
    34741 ccagccattg gatgtccacc gacaaaccga agatttttct gctcagcgga tgtcacgact
    34801 tgctgcttcg tactgctgac atccgtaatg atgacgtgat cagacaagtt gattctttca
    34861 aggacatcaa gataagctaa ggttgtacgg acgggaacgg ctaataagat aacgtctgct
    34921 tgttctgcac ctgtttcaaa atcacttgca acgtaatcaa cgatctgctg ttctactgct
    34981 tgctcacggg tttttgactg actatcccaa ccaactacta tcagttctgg attggtctga
    35041 cgaatacaaa gtgccataga agcaccaatc agtccaagcc ccacgataaa aacatgttgt
    35101 gccatcttat ttgccccctt tttagtaatg tttcaagtag tcacggtact ccttcactgc
    35161 agctttcaac tcaacaagcg aatcacttgg gaattttgac aatagctctg tcgctaccac
    35221 agtggctaca acattttccg caacgacgct agctgcagga actgctgtac tatccgaacg
    35281 ttctacactt gctttgtaag gttcttttgt atcgatatca acactttgaa gtggcttgta
    35341 caatgtagga atgggtttca tgaccccgcg aatgacaagt ggttcgccat tggtcattcc
    35401 gccttcgaag ccgcctagat tattggtacg tcggaaatag cctctttctt cttcccatag
    35461 gatctcatcc atcacttctg agcctggtaa tgaaccagaa gtgaatccga ccccaaattc
    35521 tacgccttta aaggcattga tactaaccac agcttgtgcg attttggcat ctagcttcgt
    35581 gtcccattgt acgtagcttc ctaatccagc tggaacgcct ccaacgagca cttctacaat
    35641 tcctccgatc gtatctccat ttttctttgt ctgatcgatc aaatctttta ttctttgctc
    35701 aactgctgga tcaacaactc gtacttctga cgcttcagaa atttctttga tttcttgaat
    35761 cgagatattt ccaggtattt ctgcttggat tccaccaaga caaatcacgt gtcctgctac
    35821 gatgatgtca agttcttcca gaattttctt ggcaattgcg ccgattgcta cacgcatggt
    35881 cgtctctctt gcagaggacc gttcaagcac attccgaaga tctcgatgat ggtatttcat
    35941 accgcctacc aaatccgcgt gtcctggacg cggacgtgca actcgccgca gtgattggtc
    36001 tttttcaggc acttctgcta cagacatgac agttgtccaa tttttccaat ctttattttc
    36061 taccactaaa gtgacaggtg aaccaattgt tttgccgtga cggatgccag acgtgatccg
    36121 aacacgatct ttttcgatct tcatccttcc tcctctgccg taaccagctt gtctgcgagc
    36181 gagttcgtga ttgatttctt cttctgacag aggtaatcct gctggtagtc cttcaataat
    36241 ggctgttaat tctggtccat gggattctcc cgcagtcata taacgcatct tacagtcctc
    36301 ctaagtaatc tttgaatgtc tctgtgggaa caggaacgat ttttgcttgc ccaatctttt
    36361 ctagcaagat gatattcaat tggcttcctc ttgctttctt atcatgggtt atcgcgtgga
    36421 ataattcatc tggatgactt agatcgtacg taatcggcag atggaatttt tgaagtatcc
    36481 tttctaaagc agctgtcgat ccagttggag acaagccgat tttttctgca tgagaagtga
    36541 tggctaccat tccaatagct actccttctc catgactgac taccccatat cctgctgtat
    36601 tttcaattgc atggccaatt gtatgaccaa aatttaaaat aagtcgattt ccttggtcaa
    36661 agacatcttc ttccactact tttcgtttga tttccaacgt agcagtaatg attggaacag
    36721 catgttccag aagatctttt tcgtctttca gctcgttcag ctgtttccaa agcgttgcat
    36781 ctgctatagc tgctgatttg atgatttcag cgattccttc tcgtaaacga cgtaccggca
    36841 aagtttttag aacctcagga tcaatcaata caccagctgg ctggctaaat gttccgacaa
    36901 ggtttttcgc tttgtttgta ttgactgctg tcttcccgcc aatactagaa tctacttgtg
    36961 caagtagcgt agtcggaatt tgaacgaaat ggatcccacg catgaacgtc gaggccacga
    37021 atgctgctag atcacccacc attcctccac caagtgcaag tatcccatcg ctgcgggtaa
    37081 aatcattatc tgctaaaaaa tcgtataact cagctgcttg ttctaaagat ttactggtct
    37141 cacccgccgg tacgattgaa aggactggtt caaaattagc ttgtctcaat tggaacatga
    37201 ttttttctgc atatagtgga gcaacgtttg tatcagtgat cacagctact ttttgaggtc
    37261 tccaaatcgt ttgaagccat gatccagcct gttctagtgc gcctttttca atatgaatat
    37321 cgtatttttg gttatcaaca tgtacagtaa gcattcgttt cacttctttc ttttaaaaag
    37381 ctggaattaa tcgagtgtct gaatcttttc cattgcttct ttcatgatcg caacttcttt
    37441 catcatgcgt gagtaagtct tttcattcaa cgattgggga ccgtctgacc aagcatttgc
    37501 aggatcagga tggatctcta cgatcatgcc atctgctccg cttgccacac ccgcacgagc
    37561 cattggtggt acaagttccc aaattcctgt accatgactt ggatcaacaa tcacagggaa
    37621 atgagttaat tttttcatca atgggacagc acttaaatca aacgtattac gtgtcgctgt
    37681 ttcgtaagtc cgaatcccac gttcgataaa aatgacagga gatttgtctt cgactgcaat
    37741 gtattctgca gcattaagcc attcattgat cgtaccggaa attccacgtt tcaatccaac
    37801 aggtttacct gttttaccga cagcagatag cagtttaaaa ttttgcatat tacgtgcgcc
    37861 aatctgtagg atatcgctgt actcagccac catatcgata tgcccttcat ccatcacttc
    37921 agtaatcact ttcattccaa attcatcagc cgcttgacgg atgtatttca atccttcttc
    37981 ttctaaacct tggaaagcat atggagaagt acgtggtttg aaggcaccac ctcgcagaat
    38041 ttttgcgccc ccggctttgg ccattcgcgc acattctctg atttggtcta atccttcgat
    38101 agaacaagga ccagccatcg tcacgaaact gccgtcacca atttttactc cgtctacatc
    38161 cacgactgta ttgccaggat ggaattctcg agaagtcaat ttgtatgtgt ttaaaatacg
    38221 cacagcattt tctactcctt catagcgtgt aaatgctcct tcttgtaaac ttcgcgtatc
    38281 accttttagt ccaatcacta ctcgttctgt tccttgatcg attgagactt tgaatccttc
    38341 tttttcgata tgatcgacga ctttttttat ttgttcttcc gttgcttctt ttttcataat
    38401 tactatcatt gtttttcccg ctttcttttg tttacttatc tatcttggct gattttctct
    38461 actttttcaa ggtggtttta cattaattcc tactaaatga ccgtgtcctg ttgttctcat
    38521 tttcgctcct tctttcctac ttatgaccca gataatcaac aaaaaaagat gtccctgtag
    38581 gaaattgcct ctacagtgaa catcttcaac gagccttaaa aagcgctgca aaaatataaa
    38641 atagccacta tagatgaatt tctctacgtg gctgtaagtc gtgtttaaaa atctttagcc
    38701 atcatagata caaacctggt ttgtttgtac ccacgaagtc atgaatacaa acctatctaa
    38761 aatagctaaa gtaatacgta ttcagttttg taagttttct agatttcata aaaaatcgct
    38821 ccaaactgat gtaagaattg tacctagtat agacataaaa aaatcgactg tcaatcattt
    38881 ttatgaaact ttttttaatt ttctgatagt taactttgtt tttcagaaaa ataacaattg
    38941 gttttattga agaaaatagt catgaacaaa agtatctgag aactttttta ctctcttgtt
    39001 catgacatat agaataaagc agctttgtct tgtaaactcc gtttttcaaa taataatcta
    39061 aagattgcta ctgaataata aagccgattt catggtcctc ttcgctgctg attcggtaag
    39121 gtgactcaac atccgctccc cagctattga tacccccgac tcctcttact tttgcaaaaa
    39181 tcgtaagaac cgttcgtctt tctggcggta gttcttctat atgtgttgcc tgttccagtt
    39241 cttctgcttt gaatggtaaa gcggaaaaag caaatggcgt atcctgctgt tcaaatcgca
    39301 acgaaaaatc ttcttgatct ttatccgtat tatccaacgt ttgatttctt gttagttcta
    39361 cccatttcgt ttccatatgc atgccgcatt cttggggaac aaggtatggg gaaacgggga
    39421 gtcctgccac tttataaata ccttcgacac caccagccat ccgatccgga tatgtttccc
    39481 ctgataggcc ttcatactca aaaaattttg ctttcgttgg cataatgaac cttaaaccca
    39541 atgcgggtaa ttccggtaat cctttttgtc cataataatg catattgaca agcattttac
    39601 cctctccagc gatttggtag gaaattgtca cttgggtttt tggtactgtg ttgatttcca
    39661 gtgtaaaaat gatttcaacc gtttcagcgt attcattact tgaatattga ttatttactg
    39721 gagcaatcgg caaatcgatc tttttattgt ccaccagtac ttctatttct ttgacttttg
    39781 gatacaatcc ggctcctaac cacataccgg atttaaatcc aaatccgcta cctctgtcat
    39841 tatcggtcgt tgctcgccaa aaaactggaa gtgtttcccg gtatagccat tcttttccgt
    39901 ttttattgag cgattccagt ccgcctcttg tataagaaaa aatataatgc gtattccccg
    39961 atttgacacc taatgtacag tcgccgaaga cgattgcaac tttttccgta tccatatgac
    40021 ttttatttac tgtatttttc ataataatat cttacctcct gcatggcggg tttttcttgt
    40081 ccatctgcaa aaacgatccc attccctgag aattcatagt cacttggttt atcgtcaaag
    40141 tcaccgccat agcgcatcac ttctctgcct gtgattggat cagtcacttg gatcgcctga
    40201 tctttaaaat cccatatata gcctccttgg tacatttcat aacgatcaag caacgtcatg
    40261 taagaatcca ttccacctaa cgaattcccc atgtcatgca tatattcaca taagatgaac
    40321 ggtttttccg ctttattttc aaggtagtca atgatctctt ctggtggcgc atacatgcag
    40381 ctttttacat ctgaaatcga atcttcgaat gctggatttg gaaaattccc ttcataatgg
    40441 acaagtcgat cggggtctac ttttttgaag taatcgttca tttgtcgaat gttttcttcc
    40501 gcataagatt catttccaag agaccaaaaa agaatacttg gatggttttt taatgtttcg
    40561 aaattcgttt ttgctcgatc aagaacagcc gcctgccact gaggtactgc acctggtacg
    40621 ttgtaagaag gctcaaatgc gcccattttt tgccatgagc catgggattc gaggttcgtt
    40681 tccgccatca catggattcc tgcttggtcg cataaataat accaagtgat ctggttgggg
    40741 taatgagatg tccgtacgcc gttgatcttt gcatttttta tgatagccaa atctttttcc
    40801 atatcttcta tagtgatggc gcgtcctttt ttcggatgcc actcatgtcg atttaccccg
    40861 ttgataatca tccgttttcc attgaagtag atgattttat ctttgatttc gattttacga
    40921 aaaccgaagg aatagtcaat atactccgtt actctttttt cttcgtctat cgtctccaat
    40981 tcaagcttat ataaattggg ctgaaaatgc ccccacagtg ccacattttc aaatccatcg
    41041 atgcgaatcg tcatttgctc ttttaacgga tattgctgtt gatagatttc cttcccatca
    41101 ggatctagta tcgctacatt cagctgactg tttttggcta cttgctcaaa agacaaatct
    41161 acttcaagtt tgccattttg acatttgtca tctaagcgag gtttgatcca aagatcttct
    41221 aaatggtttt gtcctcgagc aactaagaag acatctctga agatcccaaa aaatcggaaa
    41281 aagtcctgat cttccaagaa tgcagctgta cttcttttgt aaacctccac agctagaata
    41341 ttttcttctt ccaaataagg tgttaaatca aattcagacg gagtgaacga atcttcggca
    41401 tacccgataa aatgaccatt caaccaaata aacattgctt gttctacacc agcaaaatga
    41461 atcgagattt gttttccctt tagaccttct tctaatcgaa aagtcgtcaa atacgaacca
    41521 accggattgt ctttagcagc tgaaaagatc ccttccgcct ctttctcagg gcttgtcgtg
    41581 aaagggggac ggcgaaatac atgtccctcc cacggataca tcgtattgat gtaatggatc
    41641 ttgtcataat ttgctaattc aatatgacta ggaacagtga tttcatcaaa agaacaggca
    41701 tcaaaatctt ttttataaaa tgtttttggt cgtaccgaag gatttggcga ataacaaaaa
    41761 cgccatgatc catttaaaga ctgtttaagg gaggatgtgg tttctacttc ttcccttgtt
    41821 gcataacaaa catgatcgct atgtgcattt gattggttca ctctaaaaac ttgtggatca
    41881 tctaaccatt ttatatttgc tttcatactt ttttccttct ctctcataac tttttgaaat
    41941 gaagcagttc tgtttggaaa tcaccttgaa aatcggggaa gtaaagtcca actgtcatca
    42001 attcggcccc cgaaaatatc cgcttcgagg tctgttcctg ataaagtgcc tcatcatcta
    42061 accccatcag atatacttca tggaacgctg gttgagctga agctagtatc ctccctaaaa
    42121 acacaatagc ttcatcttgt ttaggagaca caaacatcca tgcggcaata ttttcttcaa
    42181 aaggactcgc caaacgataa taatggccat actggatcac cggacgaatc gtttgatagg
    42241 atataatttg gtcttttact tgcttttttt cttcaggaga cagctttgtc aaatccagtt
    42301 cataaccaaa aacagcgctc atagctgttt ctcctcttgt tttcaacgaa gtagatctgt
    42361 cggtctgatg atttggtaca gctgatacat gagctgtgat ggacgaaatc ggataagcaa
    42421 ggcttgttcc atattggatt ttcatacgct ccactgcatc cgtgttatca cttgtccatg
    42481 actgcggcat ataataaata aacccggcat caaaccgtcc acctccacca gaacagcctt
    42541 cccataagat ttccggataa tcagttgtca aatgttcaag tagttcataa agcccgagca
    42601 tataacgatg cgctacttct ccttgtctct gtgggtcaag ggtaatcgaa tagacatctg
    42661 aaagcgaacg gttcatatcc catttgatgt agtcaagagg aatcgtatct aaaatctttc
    42721 tcatttgttt ctcaatcgtc tgccgaacat ctaaccttgt aaaatccagt acatgttgtg
    42781 atctggaagc agaaggcatt ctacctggct cctgcataag aaaatcagga tgtgttcgat
    42841 acagttcaga atcaaccgag atcatttcag gttcaaacca taaaccaaat ttcaatcctt
    42901 tgctatgagc ataatctgct atttcacgta atccatttgt caacttgcct tcatactcga
    42961 accagtctcc taatgaagaa ttgtccgcat ctctttttcc aaaccagcca tcatctaata
    43021 cgaacatttc gatgcctagc tcctttgctt catcaatgat cgccttgatt ttttcactat
    43081 tgaagtcaaa gtaagtggct tcccagttat tgaccagaat cggccgttca gcaaattgat
    43141 gacgacctct tgcgattctt tcacgtagca actcatggtg gatctgactc attttgttca
    43201 atccctgact tgagtaagaa aggatcattt caggtgtttg aaaagactgg cctgcattca
    43261 attcccacga aaaacgatgg ctgttgatcc caccgacgac tctcaattga ttgatctgat
    43321 ctttttcaag ttcgaaagaa tggttgccgg aataaacaaa ttgaagtccg atcgcttctc
    43381 cagtattttc tgtcgtatga tgatgaacta aagcaataaa gttattcata tggtgactgc
    43441 tcgttcctcg acggctttca aatacttttg ttccagataa aacagattgc cgtgaaatct
    43501 gtctttcatt gacatgtgct cctggcaaag caatgacttc atcaaatcgc ctagtatggg
    43561 caaagtctag ctggaaacta gccgcttttt ctagtttgat tgtttctccg gtttcatttc
    43621 ttatctttac agatcgtgtg atcactgctc tatcagtaaa tatggtatag aacaaatcga
    43681 aataaatcgc tgtttctcta tccttaagtg aaatcaccaa cgtttgtgcc tctgacttgt
    43741 ccatcacata agtttgaggc aagccttcga tctctggctt cccatctgta atataatggc
    43801 tgtcatatct cagatccagt aagtctgatc cattcttgcg ctgaatgatc gatgcgggcg
    43861 ttcgaaagtc catgctgcca tgagaagagt attcttgcgg tagtgtatct ttcgacagag
    43921 accggtcagg atttaaggga acatttcctg aaaatccacg atctcttcgg ggataatttt
    43981 tattgtcttt gtactgcttg actggtttgc caaaataaat atgcgccaat acattgcttt
    44041 cttctatctt tatcagataa gaaagaaatg tatttcgtaa atggaagact cggctctttt
    44101 catcaaataa gatgaaattt ttatctgttt ttctatgcgt aatatccatc atttcactcc
    44161 caacaaaacg gaaaccgttt actttttttt ataattcctg tatactgttt actaaacaat
    44221 tttgtcaata aaaatttttt atttttttga ggagtgtgta aaaatggttg gaattagaga
    44281 tgtcgcacga aaggcaaatg tctctcctgc tacagtttcc cgtgtgttga ataacgatca
    44341 gacgatccat gtggctcaga ctactcggca acggatagta gaggcagctg ctgcgttgga
    44401 ttatgacatc agtactcgta aatatatcaa aaagagaatg ccttcgattg gtgtgatctc
    44461 tacgatcggc aaagcgagcg aagaacagga tatctattac aaacatttgc gagtagggat
    44521 ggaagaagaa gctcgacgtc tgcatttagg gatgaatcga atctataatc tatctgataa
    44581 tcctaaacaa tgggaagatc tagatcagtt aggtgcggtg atcgttgttg ggactgttac
    44641 gaaagaatcg attaccgcct tgttaaaaca aaacatgcaa atagtcgtcg tggataaccc
    44701 tgacatccag caagaggtcg atatggtgta tgcagatctt gaacggatga ctaaaaccgt
    44761 actggaattg ttcttagaaa atggtcataa aagaatagcc tatatcgggg gatataaagt
    44821 cgatatggat gaattcggtg ggaagcagat ttctataaat gaaaagagga tgcgtgccta
    44881 tcagcatttt atgatcgatc atcacttaga aaaatatatg tcttatcatc taggtggctg
    44941 gacagaaaaa caaggtgagg tacttgcgac taatctatta gaaaatgaat cccctcttcc
    45001 tactgccatt cttgtcggga gcgatccaat ggcaattggt gtctatcggg cattgaaaca
    45061 aaaaaatatc caaattggag aagagattgt tgtagcaagc tttgataata tcgagacaat
    45121 cgcaaacctc tcccctggct tgaccagtgt tcaaatcaat gcgaaatcaa ttgggcaagc
    45181 agctgttcga atggcattgg agcgaattga cggtatacgg gacgaaccaa tcattttgac
    45241 ttatcctgca cgattgattg ttagggaaag ttttatccca agaacaaaaa ggtattgaaa
    45301 gagaggttgt gaaaagccgc ttcgtttctt catactcaaa acgaaacggc tttttcacaa
    45361 cctcttactt atacttattc acaaaatcca atgtctgctg gtaacaattt tgcgattctt
    45421 ttagcgtgta atcaaattgg tattcatggg taatctcttt ttttgtatct gtataaaaca
    45481 aggattgcac gggtaccttt aattctgtca accgattttc taatgcttga ccttgctcct
    45541 ggaatgagta tgcatttccg tcagttatat acgttggcgg aaattcctta gaaacatggt
    45601 cagccacact agcttctttc aattgctcac tcgttttcca atcttttgtt ccaatcaacg
    45661 accacgcaac ggttttgacg aaaaacttca taaatcgatc atccgaatgc tgctgtgctg
    45721 ttagcttcaa atcaacaggt ccgcaataag aaatggttcc tttcagacta tcttttggaa
    45781 gtagctgttt gattttcatt tgtttagcat acgcatcatt cgtttgtata gctgcatatt
    45841 gcaatgcgat ttgtgaacct gcactatctc caccaaaaaa taattgttcc atgtctagca
    45901 atttgtcgtc tttttcttca gctaataaat ctttgatcag ttcattcact tgaagaactt
    45961 gattagggta ttctgaatca ggcgctaatt cataattcat cgcaaccact gcgatgttgg
    46021 cgtctgaaac taattttgtc gcaaattctt tgacacccga tttatctccg ccgacgaatc
    46081 ctccaccatg tacccaaaat agaacaggaa caggcccctt gctgtttttt ggatagtaga
    46141 tgtcataagt attctcttta tacttggact catacgtcaa atctgttttt ttcgccacgt
    46201 ttttttctgc ttgatcgaac gtttttttat cagtgatttg aacagtattt gaaaacatat
    46261 ggttgatcaa gaatgctcca ggtctggggg aaagctggaa ggcaagaaag attgcaaacg
    46321 cgatcccaag aatgattccg aagaaccatc cgataaattt aagaattttc tttttcataa
    46381 ggctccctca gtagttagta ttaagcattc tgattatacc atagtgttat ttcctatcaa
    46441 gctctcctag atgatttaat gattttcccc aaacttcttc tgcaatgctt ttgacaaaag
    46501 aaattttttc cattgttctt caaacaaaac attttcttct tctgttgaag caaatccaca
    46561 ttgaggactt aagcacaaac gatccaaagg cagatatcgt gtcgcttcgt agatccggtc
    46621 aatcacttgc tggtgctctt ccatttcgcc atttttcgtt acagtcaaaa tctaagacaa
    46681 ctaatttatc ttcagagact ttctctaaaa gaacaaaact cccagcacgt tcactatcgt
    46741 attctaaata ataggcacgg acattttctt gagtaaaata atgggtctgc tacgtcgtca
    46801 tcccccctga tgccgccaaa atcgaataat aatttccgcg tcaaacatgt atatttagtg
    46861 ttgaatcatt tgagaatatg aaaataaaaa tctttttgat caatcagtta atcaaattga
    46921 aataacgaaa aatctgagac agaatgacgt tcattctgac ccagatttga ttttgcacaa
    46981 ctcaacagtc cttattggca aatcgccaaa ataatgtaga ctaactaaaa agactaagaa
    47041 acaataacaa aagttaacac ttcttagtct ttctttagca cgtctattta ttaaagattt
    47101 tagggccttt tctcatgttt ttacgatcat tcttaggtgt ttccatttgt ttggatcggc
    47161 ctccaccttg tttctttttg atcaattctt tcattttttc tttttggtcc atttttattc
    47221 tcctttaacg aatgaagcga ctaattcgat atgatgggtt tgcgggaaaa gatctacagg
    47281 ctgaactttc ttcacggcat aacctgcttc tgcaaataac ttagcatctc ttgcaaaagt
    47341 tgccggatta catgagatat agacgattcg ttctggtgac atttcgattg ctgattcaat
    47401 aaaatgatga tctaatcctt ttcgtggagg gtcaactacg atcacagatg gtttgatccc
    47461 tttttcttgc cattttttca taactttttc tgcttttccg acttcataat gaacattttc
    47521 gattttatta tttaaagcat tgaactgtgc atcttcaatt gcttctggaa taacttccat
    47581 cccatacacc ttatccactc tgtctgctaa agacaagcca atcgtaccta ttcctgaata
    47641 agcgtcgatc acaatgtctt ttttatctaa ctcggcaaac tcgatggctg tttggtacag
    47701 atactctgct tgttgagtat ttacttgata aaatgacttt gctgagattc gataggtttt
    47761 tcctaatagc tgatcttcga tatagtctct gccatataaa actttttctt tgtctcctaa
    47821 aatcacattt gttttttctt cattgatatt ttggatgacc gaaaccactt ctggcaattc
    47881 ttcatgaata gcttcagcaa tttccttgga tttaaagaat ttttctttac gagtaactaa
    47941 aacgaccatc atttcatggg aataatgacc acgacggata atgatgtgac gaagctgtcc
    48001 ttcatttttt tcttcattat atcctcgtac ttggaaccgt tgcaagatat ctctgactac
    48061 tttgattgct tgatcgatga caggatcttg gatataaaaa tcttcgatag ggactagatc
    48121 atggctattc tttttgtaaa aaccggtggc taactgtcca tcgatacggc gaacagggat
    48181 ctgtgcttta ttacggtagc cgaatggttc tgccattccg accgttggaa gtacaggaac
    48241 ttctggcatc ttagcaatct tcgccattac atgctctact tgtttttgtt tgaagacaag
    48301 ctgttgttca tacttcaaat gggctaaagg tgcgatacct atgcgaagta atgcattgtt
    48361 ttctacaggc tgacgatcag gtgattgtgt aataaatgtt tcgacttttc catatccaaa
    48421 cttattgcct gcttttacta ctagtatttc tacttcttct ccagggagtg cattttcgat
    48481 aaataatgga taaccatcga tttttgctac ccccattcct tcataactca agtcgacgat
    48541 ttcgactgta tatcgttcgt ttttctttac tatttgctgt tctgtcatat tttgctcctt
    48601 taagcctatt tttcaatcct actgaactct actataaatc agctaattac acaactaaaa
    48661 ccggcactga ccttactacg ttcagcaccg gcttttgatt aattcttttg attaggtgtt
    48721 tcgtctgcta tttttccgtc accgtcgata tcttcatccg ttaaacgttc gacttctttt
    48781 acgaattctt tgctgacaac ctctaattct tcttcatctg tacctgtgat tgcttcatca
    48841 ggaatctgat cggtattggc aaagaactca atgtgttgat gaagattttg gaaatgcatc
    48901 ggtgcttccc cgccgtattc accatctaag ttgatcatta ctggtcgatt gatatcatct
    48961 tctgtttcaa cgaacaaact gcttgttttg gtatagatta ttcttggatc atcgacatgt
    49021 cgaccgccat tcagcatgag tgctgcaata tgcaagatct caaagatatt tgctgtcttc
    49081 acaatgatta aagaaaactt cccatcatcc aatttggcat ctggcgcgat ctgttcgaat
    49141 cctccaacag agttcgtcag ccctaggaaa aacatcgaag catttccgat atattcccct
    49201 tcatcatact tcaagcgcat tttgattggc tttactcgag ggagcatctc tgctcctttc
    49261 gcaagataag caagataacc aaaaatactc tttagttctg atggaacttc ataagtcaat
    49321 tctgtcaaat agccacctgc agcgatattg atgaagtaac tgttatcaga aaggccgatg
    49381 tccattttta cggtttggtg tttttttatc acttctgctg ctgctttaat attatctcta
    49441 ggaattttta atgctcgagc atagtcgttc gttgtaccag ctgggatgat ggccatttta
    49501 ggacggtgtt caagcggagc gattccatta accacttcgt tgattgtgcc atcgccacca
    49561 gctgccacta ctagatcaaa acctagttct gcgactctac atgcttcatt cttggctgaa
    49621 tctggttctg gtgtagttgc ataggcgctg gcttcaaatc ctgcttcttc taatgcctgt
    49681 aggatatccg ccaaattttt tttcaataat tcttttcccg aggtggggtt ataaattacg
    49741 cgtgcttttt tcattttcag cctctatcgt ttcgctaatt catctttaag tagcttattc
    49801 acgacaccag gatttgcttg tcctttagtt gctttcatga tttgaccgac taggaagcca
    49861 accgcacgat cttttccatt tttgaaatca tcgacagatt gttggttgtt atccaaaatt
    49921 tcattaatga ttggcagtag tttgttcgga tcagataatt gcacccaacc atttttttca
    49981 actacttctt gggcttctcc gccatttgtg atcaattcac ggaagacttt tttggcgatt
    50041 tttgagctga tcgttccatc ggcgatcaaa cgaatcattc ctgctaagtt ctcaggtgtc
    50101 aattttgtct cagctaattc cagtttctca ctgttcaagt aagcagaaac ttcacccatc
    50161 aaccagttag atgcttgctt ggcatctgca ccattttcca aaacagcttc aaaaaaatca
    50221 gacatttctt ttgtaagtgt caataccatt gcgtcatact ctggaagtcc aaactcacgg
    50281 atatagcgtt cgcgacgaga agctggcatc tcaggcaaac tagcttttac tttttgaatc
    50341 cattcgtcat cgatcacgaa tttcgggata tcaggctctg ggaagtaacg ataatcacta
    50401 gacccttctt tgacacgcat caaaactgtt ttattcgttg tttcgtcaaa acggcgtgtt
    50461 tcttgttgga tttctcctcc tgaaagaaga acttttgctt gacgtttttc ttcaaaagct
    50521 aaaccttttt tcacgaaaga taaagagttc aagtttttca attctgcttt cgtaccgaac
    50581 tcttcttgtc cataagggcg aagagaaata ttggcatcac agcgcataga gccttcttcc
    50641 attttcacat cgctgacttc tgtaaattgg ataattgatc gcaatgcttc tagataagcg
    50701 tatgcttctt ctggtgaacg catatcagct tcagaaacga tttcgatcaa aggcgtgcct
    50761 tgtcggttca aatcaacgta agaataaccg ccaataccat gcatgttttt tccagcatct
    50821 tcttctaaat ggacacgttc gatccggatt tttttctttt taccttctac ttcgatttcg
    50881 atccatccgt catgaccgat tggttgatca aattgggaaa tttgatatgc ttttggatta
    50941 tctgggtaaa agtaattttt ccggtcaaaa tgagtatctt tagagatttc acagtttaat
    51001 gccaaagctg ctttcatccc gaattccagc gcagctttat tcattactgg cagcacgcct
    51061 ggataacccc agtcgatcac gtttgtattg ctgtttggtt ctgcaccaaa atgagctggc
    51121 gctggagaga aaatttttga gttggttttt aactcgacgt ggacttctag tccaatgact
    51181 gtttcaaaat tcattcgtct ttccctccta aaatagctgg ttgttgttta tggaattctg
    51241 ttgcttgttc aaacgcatat gccgcttgat acatcgtttt ttcatcaaaa taattaccga
    51301 tgatctgcaa tccaactggt aatccttctg ataaacctgc cggtacagac atacctggaa
    51361 gtccagccaa gttgacaggg atcgttaaga tgtcactcat gtacattgtg attggatcgt
    51421 taatgttctc accgattccg aatgcaactg ttggtgaaga tggcccgatg atcaagtcat
    51481 aatctgcaaa gactttttca aaatctcgtt tgatcaatgt acggacttgt cctgcttttt
    51541 tgaagtaagc gtcataataa cccgcgctta aggagaatgt acctaacatg atacggcgtt
    51601 ttacttcttc accgaatcct tctgaacgtg agttgacata tacatcatct aatgatttca
    51661 cattttcaga gcgataaccg tagcggatac catcgaatcg ttgcaaattg gaacttgctt
    51721 ctgatgaggc aatgatataa taaacagcca caccgtattt tgaatgaggc aagctgactt
    51781 cttccacaac agcacctaat gaacggaatg tttctgctgc tttcaataca gcttctttta
    51841 cgccaggggc cacaccttcg cctaagtatt ctttaggtaa agcaatcttc attcctttga
    51901 tgtcgcctgt cagtccctct gcaaaatcag gaactgaaac gcctgaagaa gtaccgtctt
    51961 tttcgtcata accgctgata gctgtcaatg ctaatgcatt atctttcacc gtacgggtca
    52021 ttggtccgat ttggtcaaga gatgaagcaa aggcaatcaa accaaaacgt gagacacggc
    52081 cataagtagg tttcatccct acgatgccgt taaaagctgc tggttggcgg atacttcccc
    52141 cagtatcact acctaaagaa actggaattt gtccagctgc aactgctgct gcagaaccac
    52201 cagaagagcc accaggtact tttgttgggt cccaagcatt ttttgttttc ttgaaataag
    52261 aagtttctgt acttccaccc atagcaaatt catccatgtt tagcttacca acagggatca
    52321 tatctgcttg atagatttta tccatgaccg ttgcatcata aattggttca aaattgtaaa
    52381 ggatcttaga tgcagcggtt gtcaagatat cttttgttac gatattatcc ttgataccaa
    52441 ttggaatacc agcaaggacg ttgctttcat caatcccttt ttcatctaaa gcttttgcct
    52501 gttccaatgc tttttcttcg ttcaatgtaa tgaatgcctc gacctcaggc tctgtttctt
    52561 tgattcgttc aaatgttgtt tttgtcaaat cttgtacagt gatttctttc gatacaagca
    52621 tatgatgcag ttcttctaga gaacgattgt gtaattctgt cattatgcgc cagcctcccc
    52681 attatcgatg attgcgggta ctttgataaa gccgtcttct gtttccggca catttttcaa
    52741 taattcatcg cggttccatc cagcttgcgc ccgatcttgt cgcatcacgt ttactgtttc
    52801 aaccacattc gacgtaaatg gaacaccttc cgtatctact tcttccagca tttcaaccat
    52861 atcgatgatt ttacctaatt ggtcggtaaa acttgcaagt tcttcatctg cgaacgcaag
    52921 ttttgctaat ttggcaacgt gtttcacttg ttcttcacta atcgccatac taccccttct
    52981 ttctaaacga gttaaaaact ttgctctcta ttttaacaca agagtgccgt gtctgaaatt
    53041 gtttattgaa atttgtctga aaaaactcat ttttcattct tctactgagc gacttttagc
    53101 caatgcatct gccatttgat cttctgtcca aacttcgatt cctaattctt gtgctttggt
    53161 cagcttgctg ccagcatctt ctcctgctac cacgatatcc gtttttttcg atacgctacc
    53221 agtcactttc ccgccaagat tttcgatcgt ctcttttgct tcttcacgag taaatcgtgt
    53281 tagtttacca gtaaggacta ctgttttttc tttaaaagga gactcgactt cttgcaattg
    53341 ggcatttcga agccctttgt attcgaaatt gacgcctgct tgttttaatt cattcatcaa
    53401 ttcatggact tcttcgttac tgaaataagt gactacactg tcggcaatga tatcaccgat
    53461 cgtatcaagc gcaataatcg actcatagtc acttttactc aatgtttcta gatcaccgaa
    53521 atgttctgct aaaatttttg ctgctttcgc tccgacatgt cggattccta acccgaaaat
    53581 caaccgctcg accgaattat ctttgctgtt gtcgattgct gtcaaaatat tatttgctga
    53641 tttttctttt attttgtcca acgtcaataa ctcttcttct gtcaatttgt aaagatcagc
    53701 aacatctgct actagttttt tatcatacat ctgttctagt acgcgcggcc ccaacccatc
    53761 gatattcatc gcatttcttg aaacaaagtg gttcaaacct tctttcattt gagcagggca
    53821 tttaggattg atgcaccgta aggctacttc ttcatccaaa tggacaagtt cacttccaca
    53881 aactggacaa tgtgtaggca attggtattc ttcgctgtct ttaggtcgtt tatctaacac
    53941 gacttgcgaa acttctggga tgatgtctcc cgctttataa atcagaactg tatctttcaa
    54001 gcggatatcc ttcaacttaa tataatcacc gttatgcagg ctggcacggc ttactgtcgt
    54061 tcctgctact cggactggtg tcatgatagc tgttggcgtt actacccctg ttcgaccgac
    54121 tgtccattcg atgttttcga ttagcgtttc aacttcttct ggcggaaact tgtaagctgc
    54181 tgcccatctc ggtgctttga tagtaaaacc aagttgatct tgtagcgaga attcattcac
    54241 tttgatgacg attccatcga tttcataggg caaatccaca cgtttgtcat gatattcttc
    54301 aatgtatgac cacacttcat caatcgaatg acatagacgt ttttcccgat tcgtgtggaa
    54361 tccaattttt tctagctctt ctagcgcatc atactgggtt tttgcttgca taggaccgaa
    54421 atcagctact gtgtataaaa aagtgtccag atttcttttc gctgtgattt tagaatctaa
    54481 ctgacgcaaa gaaccagctg cggcgttccg aggattcgca aagatatctt ttccctctgc
    54541 ttctctgtct tggttcaatt ggacaaatga ccgttttggc atgaaacatt cgcctcttac
    54601 ttcgatgttc attggttctt ttaatttgat tgggaccgac cggacagttt ttaggttttc
    54661 agtgatgttt tctcctacag ttccatcccc gcgagtcgca cctctcacga aatttccatc
    54721 ttcataacgc aaagaaacgg aaagtccatc tatttttagt tcacagcaat agtcaactac
    54781 acgtcccact gctttttgta cacgttgatc aaaagctatc agctcttctt tgctgaaaac
    54841 gtcgttcaaa ctgtaaagcg gaatatcatg agtgactttt tcaaaacctt ctaatacttt
    54901 tccgcctact cgttgagtgg gagaatcaga cgtgatcaaa tcaggatatt gttcctcgat
    54961 tgctactaat tctgcgtatt ctttatcata aacatagtct tccactgttg gtttatcttc
    55021 tacgtagtat tcgcgagacc attggttcag tcttgtacga agttcttctg ctcgtttagc
    55081 agcttcgtca atcgtcattt catgttccat catttaccct ccactactaa gctttttcaa
    55141 ttggcgcaaa ggcagccaaa agacgtttta ctccttgact tgggaaagcc acatctagtt
    55201 ctagatcctt cgacgtcccg cttactcgga cgactgttcc tagaccccat tttttgtgtt
    55261 tgactttatc tccaggtttc cagctgttat tttcccctcc agtagcactt ttagagggaa
    55321 caaccgctgt ctctggctgt ttatagctat atgctgtttt tttagcgaat acaggcgttt
    55381 ttttaggcgt aggacgcatg ccttcgattt ccagtaactc ctgatcgatt tcttctacaa
    55441 aacgagaagg tcggttatat tgcgtccgtc catataatgt tctggagaaa gcatttgtta
    55501 aatacaacgc ttcttctgct cgcgtgatcc ccacatatgc gagacggcgt tcttcctcca
    55561 gttcgctttc ttccatcaat gctcgagata aaggaaaaac cccctcttct agaccaatca
    55621 ggaatacaac tggaaattct aatcctttgg ctgcatgcaa agtcatcaat gtcacttggg
    55681 aagcatcttc ttctaaattg tcaatgtcgg aaacaagtgc cagatcattt aagaagaccg
    55741 ttagtttttc ttctggtgca tcagcgtctt cttcattttg ctgttcgaat tgtttgtcga
    55801 attcttgtgt gaccgttaaa aattcctcca agttttcgat gcgtgcttgt gcttctaatg
    55861 tattttggat ttttaagtct tctagatatc cgctacgatc caaaacctct ttcgtcaatt
    55921 ctgtcactgt caagtatggg atcatctgag tgacttcttg gatcatctct ccaaatgcac
    55981 ctaattgctg tcctgctttt ccaccgatat ttgccagatc cacattttgc gctgcttcaa
    56041 gcagtggcca ttcatgaaga gaagcaaaac tgcgtagttt ttcgatagat cctggtccga
    56101 tgcctcgttt gggcgaatta acgatacgct caaaactgat tgaatcttga ggatttacca
    56161 gcacattcaa atacgctaaa atatctttga tttctttacg atcgtagaac ttgtgccctc
    56221 caaccatttt ataaggaata tttgctttca acagagtttc ttccattaca cgagattgtg
    56281 cgttcgtccg gtacaagatg gcaaagtcgc cgtaattccg atgattgctt cgaatctctt
    56341 cttgcatccg gtctacgata aaacgggttt cgtctcgttc attatctgca cggtagtaag
    56401 tgatcttgtt tccttctttg ttttctgtcc acaagttttt tggttttcga ttgctattgt
    56461 tttcaatgac ttggtttgct gcgctaagga tgtttttcgt ggagcgatag ttttgttcta
    56521 gcaaaattac tgcagcatct ggataatctt tttcaaaatc caagatgttc tgcatatctg
    56581 caccgcgcca gccgtaaata ctttgatctg catctcccac gacgcacagg ttcctaaatc
    56641 tacctgccaa taaattgact agcgtatact gtgcgtgatt tgtatcttgg tactcatcta
    56701 catgaatata atggaatttg ttttgataat acgtgagtga atcaggatgt tcttcaaata
    56761 atcgaatcgt attcatgatc aggtcgtcaa agtccataca ttgattatat tgaagctctt
    56821 tttggtaagc agcataacat tttgccgcaa tctcttcgta ataactgcct tgcatttttg
    56881 caaaatcctg aggggtctgc aggctgtttt ttgcttgaga gattgttcct aatatacttc
    56941 gaggatcata ttttttcgga tcaatgttca gttcttttag tatccgtttc atcaaggtca
    57001 gctgttcaga agaatcgatg attgtaaagt tccggttata cccgataaaa tcaacatctc
    57061 ttcttaaaat acgcacacac atcgagtgaa aagtcgatac ccagacatct tcgcctccag
    57121 atgcgagaat cgcattgaca cgttctttca tttcttttgc agccttattg gtaaaagtaa
    57181 tcgctaggat gttccaagga ttgacttctt tttcctcaat caaataggca atgcggtgtg
    57241 tcagcactct cgtctttcca cttcctgcac ctgccattag cagtaatggt ccgtcagtgt
    57301 gaagaacagc ttctttctga cgggggttca tcccgtttaa taattcagct ttggatgaca
    57361 ttaacaacat cctttctttt atcaatcttg ttaattatag cattttcttg gctttcatta
    57421 aactagctag ttcaaaatca tttgtttctt tttatggatt tttccttgtc aattcgttga
    57481 tttcgcgcta aattaaaaga aagcgaggcg tttgaatgga aaaaaaagga aaaatcaaga
    57541 aattagataa gactcagata tattttattc taaaaggaat atttgttgga ctgctgacag
    57601 gaatcgtcgt tagcctgttt cgattatcta tcgaaaagct aagcgattta gttcgaagta
    57661 tctatgaaat gagtcgggaa cagccgattt acttaattgg cctagctatt tgctgcatcc
    57721 ttgcagcatt tttcgtgggt tatctggtca aaaatgaacc ggatatcaag ggaagcggga
    57781 ttccacaagt agagggacag cttcgcgggg aattatcaat gaactggttc tctgttttat
    57841 ggaaaaagtt cattggcggt gtactttcag ttggtgcggg cctttttcta ggtagagaag
    57901 gaccttctat acagcttggc gcatccattg gacaaggagc tggacaattg tttcggagtc
    57961 catcttcaga agaaaagatt ttaatttcta gcggtgcaag tagcggactt gcagcagcat
    58021 tcaatgctcc tatcgcagga ttactctttg ttttagaaga aatccatcac tccttttctc
    58081 cacttgtttg gctgacatca tttgcttcag caatcactgc taacttcgtc tctctttact
    58141 ttttcggctt gagacctgtt ttatatctag gtgaccttcc ttctttgccg ttgaaatatt
    58201 atggttcctt agtgatctta ggagtacttc taggcatctt aggttttatt tatcagaatg
    58261 tcttgctggc tttgccaaaa ttttacaacc gattgccttt accagcttat tttcacgggt
    58321 tcgttccttt cttgctgatt ttgccaattg gttatttatg gcctagtact ctaggcggag
    58381 gcaatcaaat cgttttagct tttggacatg cttccttgcc aatcattatg attatcggtt
    58441 tgtttgttct tcgattcgtt ttttccatga tttcttacgg tgggggtctt ccagggggga
    58501 ttttccttcc gatattgaca ttgggggctt taatcggtag cttttatgga aacattatgg
    58561 ttgatttcgg gatggatcca atctacatca aggattttgt cgttattgcc atggctggtt
    58621 atttcacagc gattggaaaa gcaccgctga cagcaattat tttagtgaca gaaatggttg
    58681 gctctcttaa tcatctgatg cctttaggat tggctgctct tgttgcttat atcgtcaatg
    58741 accttctcgg cggtaatcca atttacgaat cattattgga acgtctactt ggaaatactc
    58801 agaccgcctc actaaaaggt gcaaggacaa cttttgattt tccagtaacg gcagagagtt
    58861 ctttagacgg attaatggta cgagacttca attggcctaa aaatatgctg ctaatctcta
    58921 tacgcagagg aaatcaggaa atcttgactc acggtgatac gatcatgaat gtaggagata
    58981 ttttggttat cttgactgat gaagcaaact tgccaagaat caagcaggaa atcgaggcta
    59041 aatcttctat cacaaatttg aagcaatcat tttaacgaac atggaaaaac tattttacaa
    59101 caactattca tctacactac ttgattctat atgattctat aaaaaaagaa gaggctggga
    59161 aaa
//
LOCUS       DANKKG010000002        56482 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000002 DANKKG010000000
VERSION     DANKKG010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.002"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(322..825)
                     /locus_tag="PW004_000055"
     CDS             complement(322..825)
                     /locus_tag="PW004_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDL2725057.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1184..2209
                     /locus_tag="PW004_000056"
     CDS             1184..2209
                     /locus_tag="PW004_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDL2725058.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2532..3410
                     /locus_tag="PW004_000057"
     CDS             2532..3410
                     /locus_tag="PW004_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HDL2725059.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            3886..4002
                     /locus_tag="PW004_000058"
     CDS             3886..4002
                     /locus_tag="PW004_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HDL2725060.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            4930..5268
                     /locus_tag="PW004_000059"
     CDS             4930..5268
                     /locus_tag="PW004_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725061.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            5362..5592
                     /locus_tag="PW004_000060"
     CDS             5362..5592
                     /locus_tag="PW004_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725062.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            5992..6363
                     /locus_tag="PW004_000061"
     CDS             5992..6363
                     /locus_tag="PW004_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HDL2725063.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(6637..7329)
                     /locus_tag="PW004_000062"
     CDS             complement(6637..7329)
                     /locus_tag="PW004_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL2725064.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(7332..8117)
                     /locus_tag="PW004_000063"
     CDS             complement(7332..8117)
                     /locus_tag="PW004_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL2725065.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            8354..9754
                     /gene="lacG"
                     /locus_tag="PW004_000064"
     CDS             8354..9754
                     /gene="lacG"
                     /locus_tag="PW004_000064"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HDL2725066.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            9779..10096
                     /locus_tag="PW004_000065"
     CDS             9779..10096
                     /locus_tag="PW004_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDL2725067.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            10109..11782
                     /locus_tag="PW004_000066"
     CDS             10109..11782
                     /locus_tag="PW004_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HDL2725068.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            11796..13427
                     /locus_tag="PW004_000067"
     CDS             11796..13427
                     /locus_tag="PW004_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HDL2725069.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(13918..15483)
                     /gene="guaA"
                     /locus_tag="PW004_000068"
     CDS             complement(13918..15483)
                     /gene="guaA"
                     /locus_tag="PW004_000068"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDL2725070.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(15542..16465)
                     /locus_tag="PW004_000069"
     CDS             complement(15542..16465)
                     /locus_tag="PW004_000069"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HDL2725071.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            16661..17266
                     /locus_tag="PW004_000070"
     CDS             16661..17266
                     /locus_tag="PW004_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL2725072.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            17574..18590
                     /locus_tag="PW004_000071"
     CDS             17574..18590
                     /locus_tag="PW004_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDL2725073.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            18731..20032
                     /locus_tag="PW004_000072"
     CDS             18731..20032
                     /locus_tag="PW004_000072"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HDL2725074.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            20078..20740
                     /gene="deoC"
                     /locus_tag="PW004_000073"
     CDS             20078..20740
                     /gene="deoC"
                     /locus_tag="PW004_000073"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDL2725075.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            20761..21159
                     /locus_tag="PW004_000074"
     CDS             20761..21159
                     /locus_tag="PW004_000074"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDL2725076.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            21225..22307
                     /locus_tag="PW004_000075"
     CDS             21225..22307
                     /locus_tag="PW004_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2725077.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            22519..24081
                     /locus_tag="PW004_000076"
     CDS             22519..24081
                     /locus_tag="PW004_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2725078.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            24074..25207
                     /locus_tag="PW004_000077"
     CDS             24074..25207
                     /locus_tag="PW004_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2725079.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            25207..26163
                     /locus_tag="PW004_000078"
     CDS             25207..26163
                     /locus_tag="PW004_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2725080.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            26262..27428
                     /gene="deoB"
                     /locus_tag="PW004_000079"
     CDS             26262..27428
                     /gene="deoB"
                     /locus_tag="PW004_000079"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDL2725081.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            27452..28270
                     /locus_tag="PW004_000080"
     CDS             27452..28270
                     /locus_tag="PW004_000080"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDL2725082.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            28287..28994
                     /gene="deoD"
                     /locus_tag="PW004_000081"
     CDS             28287..28994
                     /gene="deoD"
                     /locus_tag="PW004_000081"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDL2725083.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(29292..29978)
                     /gene="gpmA"
                     /locus_tag="PW004_000082"
     CDS             complement(29292..29978)
                     /gene="gpmA"
                     /locus_tag="PW004_000082"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDL2725084.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(30150..30731)
                     /locus_tag="PW004_000083"
     CDS             complement(30150..30731)
                     /locus_tag="PW004_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2725085.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(30864..31547)
                     /gene="rpiA"
                     /locus_tag="PW004_000084"
     CDS             complement(30864..31547)
                     /gene="rpiA"
                     /locus_tag="PW004_000084"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HDL2725086.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            31866..32279
                     /gene="rpsL"
                     /locus_tag="PW004_000085"
     CDS             31866..32279
                     /gene="rpsL"
                     /locus_tag="PW004_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HDL2725087.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            32363..32833
                     /gene="rpsG"
                     /locus_tag="PW004_000086"
     CDS             32363..32833
                     /gene="rpsG"
                     /locus_tag="PW004_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HDL2725088.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            32927..35014
                     /gene="fusA"
                     /locus_tag="PW004_000087"
     CDS             32927..35014
                     /gene="fusA"
                     /locus_tag="PW004_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDL2725089.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            35186..36373
                     /gene="tuf"
                     /locus_tag="PW004_000088"
     CDS             35186..36373
                     /gene="tuf"
                     /locus_tag="PW004_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDL2725090.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            36780..37088
                     /gene="rpsJ"
                     /locus_tag="PW004_000089"
     CDS             36780..37088
                     /gene="rpsJ"
                     /locus_tag="PW004_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HDL2725091.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHEYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            37119..37748
                     /gene="rplC"
                     /locus_tag="PW004_000090"
     CDS             37119..37748
                     /gene="rplC"
                     /locus_tag="PW004_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HDL2725092.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            37776..38399
                     /gene="rplD"
                     /locus_tag="PW004_000091"
     CDS             37776..38399
                     /gene="rplD"
                     /locus_tag="PW004_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HDL2725093.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            38399..38689
                     /gene="rplW"
                     /locus_tag="PW004_000092"
     CDS             38399..38689
                     /gene="rplW"
                     /locus_tag="PW004_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HDL2725094.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            38728..39561
                     /gene="rplB"
                     /locus_tag="PW004_000093"
     CDS             38728..39561
                     /gene="rplB"
                     /locus_tag="PW004_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HDL2725095.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            39604..39882
                     /gene="rpsS"
                     /locus_tag="PW004_000094"
     CDS             39604..39882
                     /gene="rpsS"
                     /locus_tag="PW004_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HDL2725096.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            39905..40252
                     /gene="rplV"
                     /locus_tag="PW004_000095"
     CDS             39905..40252
                     /gene="rplV"
                     /locus_tag="PW004_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HDL2725097.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            40266..40922
                     /gene="rpsC"
                     /locus_tag="PW004_000096"
     CDS             40266..40922
                     /gene="rpsC"
                     /locus_tag="PW004_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HDL2725098.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            40925..41359
                     /gene="rplP"
                     /locus_tag="PW004_000097"
     CDS             40925..41359
                     /gene="rplP"
                     /locus_tag="PW004_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HDL2725099.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            41349..41537
                     /gene="rpmC"
                     /locus_tag="PW004_000098"
     CDS             41349..41537
                     /gene="rpmC"
                     /locus_tag="PW004_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HDL2725100.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            41562..41828
                     /gene="rpsQ"
                     /locus_tag="PW004_000099"
     CDS             41562..41828
                     /gene="rpsQ"
                     /locus_tag="PW004_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HDL2725101.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            41886..42254
                     /gene="rplN"
                     /locus_tag="PW004_000100"
     CDS             41886..42254
                     /gene="rplN"
                     /locus_tag="PW004_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HDL2725102.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            42291..42599
                     /gene="rplX"
                     /locus_tag="PW004_000101"
     CDS             42291..42599
                     /gene="rplX"
                     /locus_tag="PW004_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HDL2725103.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            42627..43166
                     /gene="rplE"
                     /locus_tag="PW004_000102"
     CDS             42627..43166
                     /gene="rplE"
                     /locus_tag="PW004_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HDL2725104.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            43185..43370
                     /locus_tag="PW004_000103"
     CDS             43185..43370
                     /locus_tag="PW004_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HDL2725105.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            43406..43804
                     /gene="rpsH"
                     /locus_tag="PW004_000104"
     CDS             43406..43804
                     /gene="rpsH"
                     /locus_tag="PW004_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HDL2725106.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            43836..44372
                     /gene="rplF"
                     /locus_tag="PW004_000105"
     CDS             43836..44372
                     /gene="rplF"
                     /locus_tag="PW004_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HDL2725107.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            44533..44889
                     /gene="rplR"
                     /locus_tag="PW004_000106"
     CDS             44533..44889
                     /gene="rplR"
                     /locus_tag="PW004_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HDL2725108.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            44910..45410
                     /gene="rpsE"
                     /locus_tag="PW004_000107"
     CDS             44910..45410
                     /gene="rpsE"
                     /locus_tag="PW004_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HDL2725109.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            45425..45604
                     /gene="rpmD"
                     /locus_tag="PW004_000108"
     CDS             45425..45604
                     /gene="rpmD"
                     /locus_tag="PW004_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HDL2725110.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            45648..46088
                     /gene="rplO"
                     /locus_tag="PW004_000109"
     CDS             45648..46088
                     /gene="rplO"
                     /locus_tag="PW004_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HDL2725111.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            46088..47383
                     /gene="secY"
                     /locus_tag="PW004_000110"
     CDS             46088..47383
                     /gene="secY"
                     /locus_tag="PW004_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HDL2725112.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            47445..48092
                     /locus_tag="PW004_000111"
     CDS             47445..48092
                     /locus_tag="PW004_000111"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HDL2725113.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            48283..48501
                     /gene="infA"
                     /locus_tag="PW004_000112"
     CDS             48283..48501
                     /gene="infA"
                     /locus_tag="PW004_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HDL2725114.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            48534..48650
                     /gene="rpmJ"
                     /locus_tag="PW004_000113"
     CDS             48534..48650
                     /gene="rpmJ"
                     /locus_tag="PW004_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HDL2725115.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            48668..49033
                     /gene="rpsM"
                     /locus_tag="PW004_000114"
     CDS             48668..49033
                     /gene="rpsM"
                     /locus_tag="PW004_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HDL2725116.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            49061..49450
                     /gene="rpsK"
                     /locus_tag="PW004_000115"
     CDS             49061..49450
                     /gene="rpsK"
                     /locus_tag="PW004_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HDL2725117.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            49530..50468
                     /locus_tag="PW004_000116"
     CDS             49530..50468
                     /locus_tag="PW004_000116"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HDL2725118.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            50496..50876
                     /gene="rplQ"
                     /locus_tag="PW004_000117"
     CDS             50496..50876
                     /gene="rplQ"
                     /locus_tag="PW004_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HDL2725119.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            51148..51987
                     /locus_tag="PW004_000118"
     CDS             51148..51987
                     /locus_tag="PW004_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDL2725120.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            51963..52832
                     /locus_tag="PW004_000119"
     CDS             51963..52832
                     /locus_tag="PW004_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDL2725121.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            52822..53619
                     /locus_tag="PW004_000120"
     CDS             52822..53619
                     /locus_tag="PW004_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDL2725122.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            53822..54568
                     /gene="truA"
                     /locus_tag="PW004_000121"
     CDS             53822..54568
                     /gene="truA"
                     /locus_tag="PW004_000121"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDL2725123.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            55233..55470
                     /locus_tag="PW004_000122"
                     /pseudo
     CDS             55233..55470
                     /locus_tag="PW004_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299509.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(55614..56390)
                     /locus_tag="PW004_000123"
     CDS             complement(55614..56390)
                     /locus_tag="PW004_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL2725124.1"
                     /translation="MNIHHIRNATAILDYGGKKFLIDPMLAEKGTLPPFISKGPFPSP
                     RKDQMNPLVDLPMPVEDIINGIDAVIVTHLHLDHWDDIAKEVLPKEIKLFVQDENDAN
                     EIRLYGFKNVEVLREDTVFESIQLVKTKGKHGRGEILNYVGNVCGIVFKHSTEKTLYF
                     TGDTVWYEAVKQTIDQYNPEIIVANAGDNHFLVGESLIMGKDDLYELHKAIPDSTIIA
                     VHMEAVNHWGLSREELKSFNKEKGISEKVLVPEDGDNIFI"
BASE COUNT        18015 a        10103 c        12072 g        16292 t
ORIGIN      
        1 ataaagtaat tacttataaa acaagctttt tatagaaaaa taatatacct gagtagggta
       61 taaattaaga cggaattcct ttctagattg agatattgac taggaagaat gttaagattt
      121 taagccttta agctaaaaaa aaaccctttt tgttaaataa ggggaatata gcctgtcagt
      181 ttcaaactgc aagtcgtcct gctgcaataa tatagtagat tagagatgaa aatagatttt
      241 tttaaaccaa tgactgaata aaaagaccgt catagataca cattggaatg tgtctattac
      301 ggtctttttt tacgaacgat tttattgaag acggacagtt cgggtcaaaa ataaaaaagc
      361 tacgatcaga aataagaata aaatgtatcc caaaataatg gctagacggt ctaacttatt
      421 tgtcgataga tgtttgttta gaagttgata ggcccataaa cctatcagtc ctcctgttgt
      481 attagttatc aaatcagtga tgtccgctac gccaataccg aataggaatt gaatcaattc
      541 tgccaagaag cttacaagga aaataatccc taatttttta gaaaaagaaa gtctcttgaa
      601 attgacattc aaaagcaccc caaaaggaaa aaagacaatc aagttataag tggtctcacg
      661 taataccgtc gtattttcaa aagaaaaagt agaaaaaggg acgagattga cattgctgta
      721 agaataagtg agaaattgtg gaagattaaa tgatagcttg aataaaatca accataatag
      781 aaagaataaa taaatcccta ataaaaattt tgatacatac cgcatagaca tctcctccat
      841 ttaaaaagta taccatcgaa aaccgctgaa aaatcataaa aaagtatgaa gtttaaggac
      901 caacgtagtt atcgtacgaa aattagaagc ttttttttgt tgggaatccg gtttctttaa
      961 caaagctaag atagcgagtt gcttggaaaa gttgctatct tttttctgtc aaaacgactc
     1021 ggaatgttcg ttttttgttc taatttctcc aagttgttta ctttttgata ctaagtttat
     1081 gaaaaatcaa gaaatagttg ttatactagg taatgctgac aaacgggatt ggcatttcat
     1141 cttggttcag ctttttttcc aattactagg aaggagggat agattgttca agacttattt
     1201 tcgtttcaat aaaatacaag ataatccttt tcgtatcatg aatctgatgc tacttatctt
     1261 tattgtatta tggttaggtt acataaatca tgattttttg atttcaagtt tttcatcatt
     1321 agggatcttt acttttttct attatcaaaa cataccgatg aaatatttgc tgaaacgtat
     1381 gtttctgatc ggttgcggac tgttggttgc tttcgttttg gggatcttat cgacttatgt
     1441 tttatggctt gaaccatttg ccgtagcttt ggttgctttt tccagcagat tcgtgttgcg
     1501 attattccat atttctaaac caggtggctt gttttttgcg atgctttctg ccatgggaac
     1561 gagcatgcag ctgccaatcg ctcaattacc gatcgtctca ctttattttt ttatgggtgt
     1621 cgtttttgcc ctgattgcgg cagtgattac aaaattactg gattcacggc ctgagcagac
     1681 aattgaaaaa gcaactttaa aagaaagatt tcacgaagag cctttagtga tcattgattc
     1741 tgttttctac agcgctgcct tatttctttc cgtttatgtc agtcacggct tgaatctgca
     1801 taatccttat tggttgacct tgtcatgtgc atcgattttg ctagcagaaa acttggatgc
     1861 gatgaagcat cgacaagtcc aatatctgat tggatcgatg ggtgggttat gtgtgtcggc
     1921 attcttgtca tttgtgccgt ttacgcagtt gcagaccatt tttctgatca cttttctata
     1981 tggaatcgca caatttctag ttgctagaaa ttatgcagtt gctaatattt ttcttaatcc
     2041 gatggcgttg atgctatcta cacttattcg tggagcatac ttgatttctt tgatcgaata
     2101 tcgtttttta gggatcgtca taggaagttt catcggttta ggtgttgcgt gggtaatgac
     2161 ggttggtttg caacattatt taacggttgt tcgtcaaaaa ttagaataga aggacaaata
     2221 tatgtgccaa aagaggttat gacaagattt ttgtcacaac ctctaattca aggtgttcaa
     2281 aagccagatt ctcagcatta agtcaaattc acacaaaaat gtggaaagga actttcaaaa
     2341 aaattttata tttattctat caaaaaattt tgtttatgat ggtaactgaa taaactttcc
     2401 tgagatggtc aatgctttcc ttgaaagtct tacagatatt ctttcttcat agaaagttta
     2461 agttttatag ttagtgtaca ataaaagtat gtttatacca tatggtgtcc ggataatcag
     2521 tcatttaaac aatgaggttc aaagatatga aaaggggaaa tttgatgaag aaaataagca
     2581 atgaaatcca aattctggca gaaactttaa ctcttcccaa agtcgttata gagcaaatga
     2641 ttgtacaagc aaaaaacatc cttgatgagg tgtttgagca atatagtcat gaactaatgg
     2701 atcctaataa agcaaaggcg actgcaaaga aaatagacaa agtctatgaa gaggaacctt
     2761 ttgcagcatt agctatttat ctatatgcag cttctcaaag ttggaaaaaa atttatcaac
     2821 cattgaagat tccaagaact atctatttgg caacaatgaa tgcttttact agatttattc
     2881 atgaacattt ccaagtaaca ggaagctatc gctttgaccg tggtttttgg atttggcatt
     2941 acactagtgg cttgatcttt cggattggtg aattggagta cgaaatgaca tattttccga
     3001 aagggtacac ggatctaaga ttagaaggaa agcagtgcct ttctcttcat attccttccg
     3061 atgcagattt atctcagaat aagataagag atagttacga aagtgccatt cgtttttttg
     3121 aactttattt tcctgattac cattaccaag tgatgtacac agatacttgg ttactatcac
     3181 ccaatcttac taaatggttg aaaaaagagt caaaaatctg tctttttgca gccgattatc
     3241 gattattatc agttgatgaa caggacgata gtggagtccc ttggatcttt ggacgagtag
     3301 atgcccaaat acatgattat ccagaaagta cgagtttaca gcgacaagca aaagagcaat
     3361 tgcttgcagg cgagcacatt ggctctggct taggtattct tacttcataa gtttagtact
     3421 tcgaaatagt ttaaaagagc gtgagacaaa agtaaagaac acttttgtct cacgctctaa
     3481 acacgtataa acggcggaag cagaagcaac tccttcggaa ataagctgaa attcacaaaa
     3541 atttgaaaag caattttcgt gaatttcttc ttatttcttg gagttaaacg cttctgtccc
     3601 agcctctttt ttagtgttct atcgaacaca cagtacgttc aataggtaaa ctagctataa
     3661 caatagatat taaagttaac ggacaagagg gcctttttta ggtagctaaa acgtaataat
     3721 gaaaatttat ttccagagat aggagtcaat cagctagtct gatcgactgt tttaacaagt
     3781 tgactttcat tttaactttt gataccattt aaaacacaaa tcgtaattgt ttcgatttgt
     3841 gttttttagc atgactacaa tcaaagaaga aagtgggtcg tccatatgga aaaaacagat
     3901 atctcaagtg cataccgccg cttgaaaagc ccaaatatca aaacacgaaa acgtgcgctg
     3961 aagatcatca aagatgtgaa acggaatagt gggaaaaggt aagaagaagg aaacttaatt
     4021 aaaaaaatag ccgaaaaacg tttttcggct attttcaata atcggttttt gtgtaaggtt
     4081 tccgaaagaa ctgctgttga ttttccattt ttaacatatg ataaattggt agaatctttc
     4141 ttttcaaatt gataccattc cctaattcca gttatatagt taactacaga accacaaagc
     4201 acgtacgttt ataaaagtat tatctgtagt tatttttcaa ttccctttca acctcttatt
     4261 ttgaccagta gtattgatca ctttaccttt taacaccttt gttcgttttt gaaacatttt
     4321 tagtcaagaa tggaaacgta actacataat caaaggtttt gcgaaatatt tcatgaaata
     4381 tttcatgtag aagcgagcaa tacggttgca aataaccatt atcgtttaat aagcagttac
     4441 gaaagctccc aatatctcta acgttcagct cactccattt cttttcttca aaaaaatcag
     4501 tgattcgtat ctctttctat ttgtcgaaaa agttaacttt aaaaagtaac gccgaaaaaa
     4561 tatgcaaaaa aataaaaaag cagttactat tgctagaata acatcaagga ataataagtt
     4621 ttggttttca gataatagtc gataagttgt gtattggaat tttagataca gtaataaagt
     4681 atttttcatg aaattttgtg agaatcacta ttacaagtat ctttaataaa attaatggtt
     4741 aaggaggaac ttaaagtaga atgtaaatga atagttgtag tcagtcgatt gtgatactag
     4801 actcaaagtt gcaggaataa gtttttcaat ttgtgattgg aagataaaaa atgaaaaaga
     4861 agggcttttt tttatacttt gttgattgta gtgtttattt tccctctgtt tagccaagta
     4921 acattttcta tgactcaacc agtttcaagt gaggaacatg ataacctttt tcgtgctaca
     4981 gaagagttta agaaatatca ggaaaatatt gttccggaga ctccattagc tagtgagcca
     5041 attactgata ttgatggaaa ccaaatagga tggtatatac agtacgaagt tatggtggta
     5101 aataatgact caatagtaaa aaatacagta ggcttagagc taacaccgat tgaaaaggtg
     5161 agttcattgt taacgtatat ttatactgat gaaagtgacg agactgagat cattttaacg
     5221 gattactcaa aaattgctac tgataaaaag tatatattgt ggatgtagac actagtgaaa
     5281 tactttatga tatagaaatt gatgaaaatt tactagatgt agcacaagaa atcgatcatc
     5341 aaaagcagga agctattcga tatgctggta gaaaagggaa aagaaaatat ttattacgtg
     5401 aatgtagcaa aagttagaga agatgaaaat gagtggaaag aattcaagag tcgctactca
     5461 attaactcga ctccgacatt tacagtttac agagaaggat cgatagaaaa aactgttttt
     5521 tggactaaag aaagtggaat gtctttagct gaagtagaag agtttttgga ttatgttagt
     5581 atgcaacagt aagggagttc ttattatgaa aaattttgac acatattatg ttattgtttt
     5641 ttcgatatct ttgctgttaa tattactaac tttaatgcag tataaagagt atccagagta
     5701 ttttatttta acttttgtat atttagtaag tagctacaca cttctatata aattcagaag
     5761 ttcttctaga gtttctaaag tttgtttctt aattagctta ttgattctat aatatttttg
     5821 atttatatag gaaattaaaa atattataga atcaacactc agagaacggg ttggttttta
     5881 atagatttaa taagtactga taatgaaatt tctcctggtt gggaaaacga agttggttac
     5941 cctgtagaag aattttttca agattacttt gcggagtatg ccgataggat aatgagagat
     6001 tacgtaacag ataaaactca agaagtcatc aatcaatccc agtcattaaa tgaattatct
     6061 gataaggcga aagaagaagc agttgccatt atttcttcag ggattagtga ctatattgat
     6121 atgagtggtg aaatcaatta tggcgataaa gacaaaattg tacgcatgat aaaagctttt
     6181 atcaaaactt ttgatgtgaa ggcagatctt aagtttaatg atacttacct agtaggaaaa
     6241 ttaattaata tccttgggac agagaaagat acggaaacgt ttatttcctc tttgtctatg
     6301 ttgtttacag ataagcattt ttcaactcag cttggtggag aggaactagt ggaaatcttt
     6361 taaccattac gcccagcatt aataaaagta tatcaaaaaa gtactcctga tgatttttaa
     6421 gattggtttg agaaataatg tgaaaacact atttttaatg ttaattatag atagagatga
     6481 acgcgtcata tggttaaaag aatgaaaaag tcaaagatac actgctatat ttcactaagg
     6541 atatagcagt gtatctttca tgtcgtttca ctggcagttt tttataaaag gaggtgtgca
     6601 tttcagttgc ttttctttgg tagttagttg aatcgtttaa gaattaacgg atgtggcaga
     6661 caggtgctct gtccaaaaac cagtaaaatc tttgatggtt ccatctttat ttagctgtgc
     6721 ctgttcaaat actgttttgt ttttaccgaa tggatcaatt gcaataattg ttccgatccc
     6781 cgcacgtttg gcagcaagta atcctgagta agcatcttcg ataacaagac aatcagccgg
     6841 agctaagctc agtttgtctg ccgctttaag aaaaatatcc ggttgcggtt tcccagggaa
     6901 agagccgtcg tcatagacaa tcttgtccca gtcaaaccag cgggctaaat cgaaaatatc
     6961 aaaataaaaa gcaacattct ctttgacagt agccgtggca atcgttaatg gaatctgttg
     7021 gtcagtcagc tggtcaagag tcgcttcaag cccttttgtc aactgaagct cgttttcatt
     7081 ttgcaagcaa agcatacgat aatagctttc tttttcttct gacaatacgg cgatctcaga
     7141 ggcggtcaaa tgctctgaaa tgaaatgttt caaaatctcc gtattcgtcc gaccatgaat
     7201 attttttaaa atatcctcgt ctgataaacc atcttttgta tgatcatgta tcatatgcag
     7261 ccaagcagct tcatgcagat gagagtccaa aaacattgtt ccgttgaaat caaatataat
     7321 ccctttcata gttacgctcc tatagtggta ttttcggttg aatcagcaat ttgcagatcg
     7381 acatattgtg catagtgctc ttgaacctcc cgcgagatct ggcgatccgt aatgagcaga
     7441 tcgaaatcat gcaaattata aaacacataa aaatcatctc gattgaattt tttattatca
     7501 acgagtagat atttcttacg ggaattattt agagcgatct gctgtgcttc tccttcttcg
     7561 gtactgtaag tagagatcgc ttcgttgtga acgccatttg aactgataaa tgcttttgta
     7621 aactttagtc ttcttaaatt gtcgttggtg atcggtccga caaatgcgcc ggtatttttc
     7681 cggaaatcac cgccaatcag tagaatttct ggtccatctg cttgagcaag aatatcaaaa
     7741 actggataat tatttgtaat gatccgaatt ttcttgttca gtaaatgcgt cgccaatagt
     7801 tcgattgttg tacctggtcc taagaaaatc gtttctccct cgtgaatcaa ggaggctgct
     7861 gtggcagcaa tttctttttt ttcttgaatc tgcagagtgg ctttttcagt gtgagaaagt
     7921 tcttgatcta aagtgtagga aagactttga gcccctccat ggacacgcag cagcttgccc
     7981 gatttttcta attcatctaa atctctgcga acggtcatat cagaaacatc taattgattg
     8041 atgatttcat taacggtaag gattcctttt ttattgacca tttctacgat agtaagcaat
     8101 ctttcttttt tcaacattgt cttcacctct aatttaagga taatctatca tataaacgga
     8161 caacagtcaa tctaattcga acaaaagtaa acatttaggg tggtttttat gtttgttaca
     8221 atagatttta ttgctgtttt ctggttttta tgattatttg tgtttgtttt tgttgacaaa
     8281 tgttttggat gcgtttacaa tgcaatcagt tagaaagagc gaaaactgat tatagatttg
     8341 aggagagatg tacatgctta aattaccaga agattttatt tttggcggag ccacagcagc
     8401 ctatcaggtt gaaggagcca ctaaggaagg tggcaaaggc gctgttgctt gggatgactt
     8461 tttagaggaa caagggcgat tcagtcccga tccggcaagt gatttttatc atcaatatgc
     8521 aaaggacatt gaattgtgtg aacgttttgg tgtaaatggt ctaagattat ccattgcttg
     8581 gagccgcatt tttcctgatg gagctgggaa accaaatcca gaaggaatcg ctttttatca
     8641 tcgtgtgttc gaagaatgta agaaaagaaa cgtaacgccg tttgtgacgt tgcatcattt
     8701 tgatacccct aaacgtttgt ttgaccatgg agattttttg aatcgagaaa caatagaagc
     8761 atttgtttcc tatgccattt tttgttttca tgaattcaaa gaagtcaagg tgtggagtac
     8821 ctttaatgaa atctatccag ttgcaaccaa tcaatatttg ttaggtgttt tcccaccagg
     8881 aatcaaatat gattttacaa agatcgtcgc ctgtcttcat aatatgatgg ttgctcatgc
     8941 ccgtgtagtg aactatttca aagaaaacga acttccgggt gaaattggtg tagttcactc
     9001 attagagacg aaatatgcgg caacagacgc gccggaagat aagcatgctg cctttttaga
     9061 cgatgcattg tcgatccgtt tcttattaga tgctacctat ttaggctatt attcaactga
     9121 aaccttgact gctcttgatg agatatgtga agcaaatcag gccagctatc attttcctga
     9181 agaagatttc gttgaattga aaaaagcatc cacgcgtaat gactacttag ggatcaatca
     9241 ctaccaatgc cattttgtca aagcttacga tggagaaaat gcaatccatc ataacggtac
     9301 gggtgaaaaa gggacctctg tttataaagt gaagggaatc ggagaacgta tttataaaga
     9361 aggaattcct cgaactgatt gggactggct gatttatcca gaaggtctat atgacttgtt
     9421 gctgcgaatt aaaagcgatt acccacatta caacaaaatc tatatcactg agaacgggat
     9481 ggggtataaa gatcagtttg aagatggcat tattatggat cagccgagaa tcgactattt
     9541 gagagtctat ttggaatcct taagcaaggc gataacagcc ggggtaaatg tgaaggggta
     9601 ttttttgtgg tcgcttatgg atcttttctc atggacaaat gggtataaca aaaggtatgg
     9661 cttgttttat gttgattttg aaacccagaa gcgttaccca aaagaatcag cttactggta
     9721 caagttggtc agtgaaacca aaacgattat ttaataagtg ataatggagg aagaaaagat
     9781 gaataaagag gaattacaaa tgcttggttt tgagatcgtt gcatattcag gcgacgctcg
     9841 cagtacatta ttgaaattat tgaaagaagt aagacaagga aactttgaac atgttgattc
     9901 ggcgttgaaa gaagccgacg aaaatttaac ccttgcacat aattcgcaaa caaaaatttt
     9961 agcggaagaa gcatctggga aagagatgga gatgggattc atttttatcc atgggcagga
    10021 tcatttaatg accactttat tgctgagaga tctgattcaa gactttattg tactgtacag
    10081 acagaatcaa ggataggaga agaacgctat ggatggaatc attaaacaaa tcgaaaaagg
    10141 gaaaccattt tttgaaaaaa tctcacgtaa tagttattta ggagccgttc gtgacggatt
    10201 tttaacagcg atgccagcta ttttattttc aagcattttc attttgattg ctgcggttcc
    10261 tgagattttt ggttaccaat ggcctgaatc tgtctccaca tggctatgga aagtctataa
    10321 ttattcgatg ggtgttgtag ggttactcgt ttcagcaacc actgctcgct gcttggctga
    10381 ttcgatgaat cgtcgcttac cgaaaaacaa agtaatcaac accacctctg tgatgctggc
    10441 ctctgtggta agtttcctgt tgttaagcgt ctctccgatt gaaggtggat tttcaacgga
    10501 atatatgggg acaaagggac tactggcttc ctttgtcgct gcatttttaa cagttaattt
    10561 atataagttt tgtgtgctga aagatattac cattaaaatg cctaaagaag ttcctggaac
    10621 gatttcgcaa accttccgag atatttttcc tttctctttt tcagtatttg cggccgtttt
    10681 aattgattta ggcattcgac aaatcttcca aatctcattt gcagaagcaa tcattacggt
    10741 gctgcagccg ctatttacag cagctgacgg gtacttagga atcgctatta tctggggagc
    10801 gatggcgctg ttttggtttg tcggtgttca tggaccgtcg atcgtggagc cggcaattgc
    10861 ggcaattatc tatgccaatg tggaaaccaa tctgcaattg ttcaattccg gtgaacaagc
    10921 ttctaaagta ctgacggtgg gtttagggaa ttttgtagga actatgggag gaactggggc
    10981 aacattggtt gttcctttta tcttcctact ctttgctaag tctaagcagt tgaaggcagt
    11041 tgggaaagct tcctttattc cagtaagttt cgctgtcaac gaaccattgc tatttgcggc
    11101 acccatgatt ttgaaccctt atttttttat tccatttttg ttaacacctg ttgtaaacgt
    11161 ttgcctgttc aagttttttg tcgatgtatt gggcatgaac agctttatgt acgttttgcc
    11221 ttgggcaact ccagcaccga tcggtttggt tctaggaacg ggcatgggta tcttagcttt
    11281 tgttctagca gcggttctaa tcgtcgttga cttcctgatc tatctaccat tttgcaaagc
    11341 atacgatgct gtcttagtag agaaagaagc acaaaaagca gaggtgatcg caaccgagga
    11401 aggtgcttct gccgatgtac gtgaggaagt acctgtggca gcgagtcaag ttatcagcga
    11461 tgcaaataaa gaaatcaatg tgttggtact ttgtgctggc gcagggacaa gtgcgatgtt
    11521 agctaatgct ttaaaaaaag gtgcatctga atcgaaagca ccgatcagtg caatggcggg
    11581 tgcttatggt tctcattacg aaatgctgca ggattttgat atggtggtct tagcaccgca
    11641 agtcaattct tactatgatg atatcaaaga agacaccgat cgcttaggaa tcaagctggt
    11701 tgcgacaaaa ggagcagaat atatcggatt aacgagagat cctgaaaaag ctgttcagtt
    11761 tattctgaac caatttcaat aaaggaggag ccataatgaa aatgggaatt tggttgcgta
    11821 aagctgtttt atttgcagct gtgttagggg ctttgggggc tgtttcaaaa actgttgaag
    11881 catctgccag cgaatttatt cgcggagcag atatttcgat cctgtcagat atggaaaaat
    11941 ctggtgcaaa atattatgaa gacgatattt caaaagatgc cttagagatt ttaaaaaata
    12001 atggtgtcaa ctatgttcga ctgcgtttgt ggcaggatcc ttataatagt aatggagaaa
    12061 gttatggagc agggacaaat gatctggaaa cgacgattga ccttgcaaaa cgagcaaaaa
    12121 atctgggact taaggtgctg ctggattttc actacagtga tttttgggtt gatcctggga
    12181 aacagaatct tcctaaagca tggcaaggtc tgacctttga agaaatgaat acggcattgt
    12241 acgactatac aaagaacgtt ctgtcggaaa tgaagcagct ggacgtttat cctgatatgg
    12301 ttcagatcgg caatgaactg aatagcggaa tgctctggcc ttatggcaag tcttggggag
    12361 aaggcggggg agaatttgat cgattggctg cttttttgaa gtctggtatc caagctgttc
    12421 gagacactca acctaaaaca actccagtaa tgctccactt agctgatggt ggcgatacag
    12481 gagcatttac ttggtggttt gatgaaatca cgagtcgagg tgtgtcattt gatttaattg
    12541 gagtgtcgta ttatccgtat tggcacggtt caatgaatga acttcaaacc aatatgaatg
    12601 cgatcagcca acggtatggt aaagaagtga tcgtagttga aacagcttat gggcatacaa
    12661 cagcgaatgc cgatactatg ccaaatgctt ttggtgaagc cgaagcagct gcggggggat
    12721 atgaaccaac accgacagga caagcagagt atttgctgga tcttgccgat cggattcagg
    12781 cagtaccgaa taatcgcggg gcgggcttct tctattggga acctctttgg tacaacggaa
    12841 atgtgagttg ggcaacacaa gcaggtatga attatctcag tgttcaatcc actatgggaa
    12901 acgaatggga caaccaagcg atgtttgatt ttgctggaaa cgccttgcca agcttgcgtg
    12961 cgtttaaaca agcaggggcg caaataaatc tagttaaaaa tgcaagtttc gaaagcgatg
    13021 gaccaacgac cagtccttcc aattggcaaa cctggtttca acaaggaacc gctaagggta
    13081 cgatcaaaac ggaagcgggc actttttttg gggactacaa actaactttt tggcaggatc
    13141 aagcatacga ggcatcagct tatcaaaatg ttagtggcat aagcaaagga acttacacat
    13201 tgagtgccta tgtaatgtct gggggagagt ttgatactaa tcagttatat atcaaaaatt
    13261 atggtggagc ggaactaaat actgcggttg ttaaaacaag tgaatggaca aaaattacaa
    13321 tcaaagacat tccaattaca acaggtgttt gtgaaattgg ggtttatagc aaggcttcag
    13381 gaaatgcttg gacgaacgtt gattttgttt cattggtgaa acaatagact gcggatcgtt
    13441 tcgattcaag atcggaggtt cccttcagcc acatcgttag cgtagaaggc aggaatcgaa
    13501 cctgcacctc gcctttgcca aaaataacca gaaacgattt ccagtcattt ttaccaaaac
    13561 gagtaaagca ccagacttca tctacacttt gtattatgct aaagagagtt caacgatttc
    13621 gtaagaagca atgaaaaaat tgcaaaaagt tgtgcagata acataaagtt tgtttaagaa
    13681 agggtaacct tagaattccg tcgtgaatat gatttgagca cttagatgga tagaaaaaag
    13741 ttctcggaaa acagttcgtt tttcgagaac ttctttttat gataagaatg aaacaaatta
    13801 acttatatta tagtctcttt tggtagggaa ccatcttatt tttaatgaaa atcaagcatt
    13861 ttagctattg aagaatgttg atttataggg attccccctc catattatgt tggctcttta
    13921 ttcccactcc actgttgctg gtggtttaga agtgatgtca tacacgatac ggttgacgtg
    13981 ggctacttcg tttacgatac gtacagagat tttttgcaag acttcccatg ggatacgggc
    14041 aaagtcagcg gtcattccgt cgattgaagt tacagcacgg ataccgactg tgtagtcgta
    14101 agtacgtccg tcacccataa caccgacaga gcggatccct ggaagaacag tgaaatattg
    14161 ccagatatca cggtcaagtc cagcagctgc gatttcttca cgcaagatag catcagattc
    14221 gcgtacgatt tctagcttgt cttcagtgat ttcgccaaga acacgaatac caagaccagg
    14281 accagggaat ggctgacgcc aaacgattga atcaggcatg cctaactgag tccctaattc
    14341 acggacttcg tctttgaata atgtgtttaa tggttcgatc aattcaaatt gcatatcttc
    14401 tggcagtcca cctacattat ggtgagattt gatcgtctgt gctgtttctg taccagattc
    14461 gataacgtca gtatacaacg ttccttgtgc taggaaagag acgccttctt caccagcaag
    14521 ttttgttgct tcgtcatcaa aaacatatac gaattcattc ccaatgattt tacgtttttg
    14581 ttctgggtca gatacacctt tcaacttgtc taagaagcga tctcttgcat tgactttgat
    14641 gatgttcaag ccgaattttc cgcctagact ttccattact tgttccgctt cgcctttgcg
    14701 tagcagaccg tggtcaacaa agatagatgt caattggtca ccgatcgctt tttgtaaaag
    14761 aaccccaacg acactagaat caacgccacc tgaaagacca agcaagactt ttttgtcacc
    14821 aacttgttca cggatttttt ctacttgcat atcgataaag ttatccattg tccagttacc
    14881 ttcacagtga caaacatcaa aggcaaagtg gcgcagcaat tcgattccgt attcggaatg
    14941 acgtacttct gcatggaatt ggataccata aaaattacgt gttgtgtctt ggatagaagc
    15001 aatcggacaa tctttgcttg ttccgactgt ttcaaagcct tcaggaactt tagttaccaa
    15061 gtcaccatgg ctcatccata ctgtttgttg ttttggtgtt tctttaaaca agacagcttg
    15121 atcgcttgtt acttctaaca aggctttccc atattcgcgg tttttagcag gctctacgga
    15181 accgccaagt ttgtacgtga tcaattgcat accatagcaa atccctaata caggaatacc
    15241 taaattgaag atctcaggat cgatcccaaa tgcattctca tcatatacac tgtttggtcc
    15301 gccagaaaga atgattccct ttggattcat cgcttttact tcttctgcag taatgcggtg
    15361 gctcaataat tcggaaaaga ctccaagatc acgaatacgt cttgtgatca attggttata
    15421 ctgactaccg tagtctaata cgataatctt ttcaacgttc gtcatatcgg caacgtttgt
    15481 caaatttatt acccctatcc ctttaaattt tatctcaaaa tagtcaaact attttaaaat
    15541 gctaatattt tcgcatgaaa atcgaatcga tgatatgatt ttcagtttta tggagaatta
    15601 tatcggctct tcctcgtgta ggcaagatat attcctgtaa atttttcaaa ttcacatttt
    15661 tccatacatc ttttgccatt ttaaaggctt cttcacgatc tccgatagca aaacgataat
    15721 aataattctc tggattttgg aaagccgtat ccagtaacgc accaaaacga tccaaatacc
    15781 atttttcgat cagcacggga tcggcatcga catagacaga aaaatcaaaa aagtcgctga
    15841 cgtagatttg ctgattcgca ggtaattgaa gcgtattgat cccctcaacg atcaagatat
    15901 caggctgctg gatcaattcg tacttgtctg gaatgatatc atacacatca tgtgagtaaa
    15961 caggtacttt tatctcttcc tgaccagatt ttacacggtt caagaaatcg atcagcatct
    16021 ccatatcata actttcagga aatcctttac ggtccatgat tccacgctct tttaatatac
    16081 gatttggata aagaaatccg tcagtcgtga tcagctgaac gtttcttcgt ttgaaggtac
    16141 gagacaaaat cgtctgcagc agtctggctg ttgtactttt tccaacggct acacttccgg
    16201 cgatcccgat aataaaaggc ggtacttcta cgtatttatg aagaaataaa cctttactca
    16261 agaacaaaga ttcaaattct ttcatataaa gataaatcag atgagcaaga ggtacataga
    16321 tatctcgaac atcctgcatg gaaatgcggt cattcaaact tttgatatta tctagttcat
    16381 cttgtgtcaa aggtgcttgc ccattacgat aaaattctcg ccattcttct cttgccacac
    16441 ggtagtagtt cattggatca ttcatattag cctcctgcgt tctcccatca atggaactat
    16501 tttaacacaa aataaacgca aaccccacca cttaagagag attgccgatc tttttgtata
    16561 atctttataa tagaaagaaa cggagtctta ttcttgactt gcgcacggat acataaaagg
    16621 atagactgtt gtcatgaaag aaatgagaag gatggaagac atgacaaatc attactattc
    16681 tgaacgccca gatacggcgc acgattttga acaatgggct tttgagttaa aaggcagaac
    16741 ctttcaattt gtaacagatt ctggtgtatt ttcacgtgaa acagtggatt atggttcacg
    16801 tgtcctgatt gatgctttcg attgggaaga gttacctgat gaaggaaaga tcttagatgt
    16861 gggatgcggg tatggaccga ttgggttagc tttggcattt gcgacacagc ggtttgttga
    16921 aatggtcgac attaatgaac gagcggtaag tctagcgcaa ggaaacgcaa agcgtaacca
    16981 aatcgaacat gttgatatcc atcaatcgaa tatctatgaa gcagttcatg aagaaaccta
    17041 tgcagctatc gtaagcaatc cgccgatccg tgctggaaaa aaagttgtac atgaaatttt
    17101 gacaggcgca tatcctcggt tgaagaaggg cggtacactg acaattgtga tccagaagaa
    17161 acaaggagcg ccgagtgcgc aaaagaaaat ggaagaaaca tttggtaatg cagaaatcgt
    17221 cacaaaagat aaaggatatt atatcatcaa aagtgtgaaa gaataactag ctgaaaaata
    17281 gtggagcgac gaaagagtgg aactgggcgt gcatgtacgg gtacgtatca gttctttttt
    17341 tgttgagaag tttttaaaaa caaagaacta ttttttcttc aaaaagaaat agtaggaaca
    17401 gagtcgttgt tttttacgat aaaataaata agatacagaa cttgtataga aggctgccaa
    17461 gtcggtagat aaaggaggtt tttatcaaaa aacatacttt ccttttttct ctattctttt
    17521 cttgggggtt ttggtacact gatagggaat ttagatagaa gggagccaat aggatgaagc
    17581 tgaccatcag agatatcgca gaaatggcag gcgtatcagt aacaactgtc tcacagattt
    17641 tgaataacaa aggaagcaga tttagtgata agacaagaaa aaaagtgttg tcgatcgtga
    17701 atgaacatca ttataagccc gattatttcg cttcaaatgt gatcaatcga cattcaaaga
    17761 ccatcggaat gatcgtccct gatgtcactg atttcttctt ctccaaactg atcgaaggtg
    17821 tagagagcta tttgaatcct ttgggctatg tgatcatgtt atgcaattct cgtcatagcc
    17881 aagaaaatga gatcaagtat ttacaagagc tttcccaccg ctcagtggac ggaatcttgc
    17941 tggccacacc aaatatttta cctgagaaat atgcattaga cagcggtttt tatcaaaaaa
    18001 tgccaatcat tttaatcgat cgcggattga atcgtcggga taacggacgg cttatcgtaa
    18061 aggaatatga gggcgcctat caagctgttt cgctgtttat tgaaaatggc catacgaaga
    18121 tcggcatgct gaaggaaacc actggatact atcaattaga agaacgtttc aacggatatc
    18181 gtcatgcgct gaaagatcat ggcctgcctt ttaatgggaa atttgttgaa catggagagt
    18241 tgactgtcca aggtggatat actgcatcaa aaaaattact caaacataaa gatatcacgg
    18301 ctattttttg cgggaatgat gccatggcga tcggatgcta tcaggcgatc gatgaattgg
    18361 gcaaaaaaat cccagaagat atctcagtca taggattcga tggattaaag ctgtcagaat
    18421 acatgattcc gcaattgacg acagtgcagc agccaagttt tgatattgga ttttatgctg
    18481 cacgtttcct gatcgatacg atcgaatttc ctcaaagaaa agttccgaac aaagtgttcg
    18541 aaacaaaatt aattattcgt gagagcgtaa aagtattgac aaagggctaa ctcggcggta
    18601 tattgcttat gtaagcattt acaaaggcta gttagtgctt tttttatttt tagtaaacca
    18661 gtttactaaa gtggtttacc tttcctaaaa ataatgtata ataccgaatg aagacaagga
    18721 ggatcaggca atgcgaatgg tggatctgat tgaaaaaaag agggatggaa acgagctgtc
    18781 gaaagaggaa atcgagtaca tcgtcacgaa ctatacgaat ggaaagattc cagattatca
    18841 agtcagtgca ttattgatgg caatttttta tcaagacatg acaaatgaag aaatcacgaa
    18901 cttaacattg gcgattgcta attctggtga tgtcatcgat ttgagttctc ttgaaggaat
    18961 aaaggtagat aagcattcga caggtggcgt tggtgacacg accacattga ttttagcccc
    19021 cctagtagct agtgtcggag tgacagtggc taaaatgtct ggacgcggtc taggttacac
    19081 aggtggtaca ctagacaagc tagaagcaat tcccggattt cagatcgagc tttcggatga
    19141 agaatttgta cgcattgtta atgaaagtaa agtagcggta attggtcaat caggaaactt
    19201 ggcaccggcg gacaaaaaat tgtatgcctt gcgtgacgtc acagcgaccg ttgattctct
    19261 tccgctgatt gccagttcga ttatgagtaa aaaaatcgcg gcaggagcag atgcaatcgt
    19321 tttggatgtg accactggag acggtgcttt catgaagaac atcgaagatg caagacgtct
    19381 ggcaaagacc atgacgagca ttgggaaact ggctaaccga gaaacagtgg cggttatctc
    19441 agatatgtca gaacctcttg gcgaagctat cggaaatagt ctagaagttg tcgaggcaat
    19501 cgaaacatta caaggaaatg gtccagaaga tctagtagaa atgtgctacg ccttaggaag
    19561 ccaaatggtt gttcttgctg gcaaagctaa gacaaccgat gaagcaagaa cgttgcttca
    19621 agaagctctg gaatcaggta aagcgttagc aaaattcaaa gaaatgatcc aaaatcaagg
    19681 gggagaccca gcgatcgtgg aacatccaga acgaattttg actgcacgct acacaatgga
    19741 gcttcctgct aaacaaagcg gagttgtgtc aaaaatcgtg gcaaatgaat taggaattgc
    19801 tgcaatgatg ctaggtgctg ggcgtaaaac caaagaagaa gatattgatc acgctgttgg
    19861 attgaaactg cataaaaaaa ttggtgacac tgtgacaaaa ggtgagtcac tattgacgat
    19921 ctatagtaac gacgaagaaa tcacttctgt cattgaatta ttatataaaa atattgaaat
    19981 tggtgaatcg gcaatgaagc caacgttgat tcatgacatc atcacagaat aaaggaaaaa
    20041 agcggacttt accgttttaa agaaaagggg aaaagaaatg gaactaaacc gaatgatcga
    20101 ccatactatt ttgaaagcag acgcatctaa agaagctgtg atgcaaatta tcgaagaagc
    20161 aaaaaaatac catttttatt ctgtctgcat caatccagca tgggttgcgc tagcgaaaga
    20221 acaattacaa ggtgagccag tagccgtatg tacagtaatc gggtttcctc taggagcaaa
    20281 cacacccgag acaaaagcat ttgaaacaac agatgccatc aataacggtg cagatgaagt
    20341 cgatatggtc atcaatatcg gtgctttgaa atcaaaggat gatcaacaag tccaaaaaga
    20401 tatcgaagca gttgttgaag cagcaaaaga tcgtgcgctt gtcaaagtta ttattgaaac
    20461 atcgttatta gatagagaag aaatcatccg agcttgcgaa atagcaaaag ctgcaggcgc
    20521 tgattttgtc aaaacatcaa ctggtttctc aacaggcgga gcaaaggtag aggatgtccg
    20581 tttgatgcgt gaaactgtag gaccagaaat gggtgtcaaa gcttccggcg gtatccataa
    20641 cgaagaagaa gcaatggcaa tgatcgaagc tggtgctaca agaatcggaa caagcgcagg
    20701 agtagcaatc gtatctggtt caactggtga aggctattaa ttttttcaaa ggagaagctc
    20761 atggcagaac tcaaacaaga atggatcaac acagctattg aagcgttaga taaagcctat
    20821 gttccttatt cccattttcc ggtaggcgct tgtctcgtaa cggaatcagg caagatttat
    20881 caaggcatca acattgagaa tgcatcattt ggattaacga attgtgccga gcgcacagct
    20941 tttttcaaag cagtatctga aggagaaaaa acatttacgc atctagtcgt agcggggcac
    21001 acccccgatc ctatctctcc ttgtggtgca tgtcgacaag taatggcaga attctgttcc
    21061 cctgatatgc cggttacgct agtaggagac aatggtgtga cgaaggcaac gaccgttcgt
    21121 gcactgctgc cttatgcatt tactgaaaaa gatctataac tcatgttcat tcagtcagct
    21181 gactgttgaa aaataaaaca attattttag gaggctttac agaaatgaaa aaagcaaaat
    21241 tatttggttt aggtgctgtc gcactagcag cagggttatt tttaggtgca tgcggcaaca
    21301 acggctctac tgattcatcc ggtggcaaaa gctcatcaga cacaacaact gcagcattga
    21361 tcacagacac aggtggtgta gacgaccgtt cattcaacca gtcagcttgg gaaggactag
    21421 aaaaatgggg gaaagatcaa ggactttcac gaggaaatga tggcttccaa tatttccaat
    21481 cttcaaatga atcagattac atcccaaata ttgaccaagc attgaatgct ggattcaaaa
    21541 cgatttttgg tattggttat aaactaaaac cagcgatcca agaacaagca acaaacaata
    21601 caggtacgaa ctttgttatt atcgatgatg tcatcgatgg attagataac gttgtatccg
    21661 caactttcaa agacaacgaa gcttcttatc ttgcaggtgt tgcagcagct tatacgactg
    21721 aaacaaacgt agtcggcttt atcggcggtg taaaaggtga agtaatcgat cgttttgatg
    21781 ctggattcaa agccggtgtt gatgcaggtg cgaaagaatt aggaaaagaa atcaaagtat
    21841 tgaatcaata tgctggtgac ttctctgctc cagataaagg acgttcaatt gctcaaggta
    21901 tgtatgcaca aaacgcagat atcatcttcc atgcttcagg cggtacaggt aacggtgtat
    21961 tccaagaagc aaaatcattg aacgaatcag gcgacaaaaa agtttgggtt atcggtgttg
    22021 accgcgacca atcagacgaa ggcgaataca cattaaacgg cgaaaagaaa aacttcacat
    22081 tgacatctac actgaaagca gttggaacag tagtagaaga tttggcacaa aaatcagcgg
    22141 acggtaaatt ccctggcggc gaacacactg tttatggatt gaaagaagac ggtgttggtc
    22201 taacagaagg acaactttct gacgaagcga aaaaagcagt tgatgaagca aaagaaaaaa
    22261 tcatttcagg tgacgtaaaa gttccagaaa ctcctgaaga aaactaaaac catttctata
    22321 gccttgttag acacatgttt gacaaggcta tggtttgatt agaacaagaa ttgttcattt
    22381 atccaagctg ccttgtgaca gtttggataa gggaacaact ccctgaagta atgtttgttt
    22441 caaaagaaga acgctcacta agcgtgaatc ttagtcaatc gttgccctag atagaaatcg
    22501 ttaggatgtg aaaaaaaggt ggttcaagaa aatattgtca tagaaatgca gaatattacc
    22561 aaagaatttg gaacattcaa ggcaaatgac aacatcaatt tacaagtaaa ggcaggagaa
    22621 attcatgcgt tgcttggaga aaacggtgct ggcaaatcta cattgatgaa cgtgctttcc
    22681 ggattattag agccgacatc agggaaaatt ttgatgcgtg ggaaagaagt aaagatcaca
    22741 agtccgacca aagccaacca attagggatt gggatggtcc atcagcactt tatgcttgtt
    22801 gatgccttta ctgtaacaga aaacatcgtg ttgggaagcg agcctagtcg cgcagggatg
    22861 cttgaccata aaaaagcacg aaaagagatc caaaaagttt ctgaacagta tggattgtca
    22921 gtcaacccag atgcttacgt tcgtgatatt tcagttggaa tggaacaacg ggtagaaatt
    22981 ttaaaaacac tttaccgagg agcagatgta ctgatttttg atgagccgac agctgtattg
    23041 acccctcaag aaattgatga attaatcgtg atcatgaagg aattagtcaa agaaggcaag
    23101 tcgatcattt tgattacgca taagttagat gaaatcaaag cagtagctga ccgctgtaca
    23161 gttatccgcc gtggaaaagg aatcggtaca gtcaacgtta aagacgtaac ctcacaacaa
    23221 ttagccgata tgatggttgg aagaacggtt tcattcaaaa cgatgaaaaa agaagcgaag
    23281 cctcaagaag tcgttttgtc tattgaaaat ctagtggtga aagaaaatcg tggattagaa
    23341 gccgtgaaaa acttgaactt agaggttcgt gctggcgaag tacttggtat cgctggaatc
    23401 gatggaaacg ggcagtcgga gttgatccaa gctttgactg gtttgcgaaa ggcagaaagc
    23461 ggacatatca agctaaaagg ggaagacatc actaataaaa aaccccgaaa aatcactgaa
    23521 catggtgtag gacacgtgcc agaagaccgt cataaatacg ggttagtcct agatatgaca
    23581 ttatctgaaa atatcgcact acaaacctat catcaaaaac cttacagtaa aaacggcatg
    23641 ctgaattatt cagtaatgaa tgaacatgcc agagaattga tcgaagaata tgatgttcga
    23701 acaacgaatg aacttgttcc cgcaaaagct ttatcaggcg gaaatcagca aaaagcaatt
    23761 atcgctcgtg aagtcgaccg agatcctgat ctgttgattg ttgcaaatcc aactcgtgga
    23821 ctggatgtag gtgcgatcga atttattcat aaacgtctga tcgaacaaag ggacaaatac
    23881 aaagcagtgt tactgatcag tttcgaatta gaagaaattt taaatgtttc ggatcgtatt
    23941 gctgttatcc atgaaggaga aattgtcggt atcgttgatc cgaaagaaac atctgaaaat
    24001 gaattaggtc tattgatggc gggctatacc ttagaagaag cacgaaaaga gttagaaaag
    24061 ggggctggcg cacatgaatg atcgcagcga aaaattccgt aatttattag tgcctgtact
    24121 ttcagttgtt ttaggattga tcttaggcgc gatattgatg gctgcttttg gttttcagcc
    24181 catccaaggt tattcttcta tgctagatgc agcccttggt tcgcaaagag ggataggtga
    24241 aacgttaagg caagcaacgc cgttgatttt tacagcatta ggtttttctg tagctaactc
    24301 agctgggttt ttcaatattg ggctttctgg acaagcactt tgcgggtgga tcgtcagtgt
    24361 ctgggtggca cttgcgtttc cagatctgcc aaaagttgtt ttgcttccgt tatgcgtgat
    24421 cttaggtgcc ttagctggtg cgatggcagc ggctgttcca ggtttacttc gagcattttt
    24481 tggaacaagt gaagtgatcg taacaatcat gatgaactat atcttattat acgtaagcac
    24541 atttgcttta catgaagtga tgcctgaaag ctatcgctca agtttggact cttccaatat
    24601 gatcagtcaa aatgcctcac tgcggttacc atggttaaca caagcttttg gcggctctcg
    24661 cgtaaactca ggattatttt tagcaatcat cgcgcttatt ttcgtttggc tgctgatgaa
    24721 gaaaacgact ttaggatttg aaatccgttc tgttggattg aatccttttg cttctgagta
    24781 tgcaggaatg agtagtaaac gtacgatcgt tttgtcgatg gtcatttctg gcgctttggc
    24841 agggcttggc ggtgtggttg aaggattagg aacgtatcaa aacttctttg ttcaaacgac
    24901 ttcgctatct attggatttg acggaatggc tgtttccttg cttggttctg gatcagcaat
    24961 cggtattctt ttatcagcga tcttgttcag tattttgaag atcggtggac ttggcatgca
    25021 gacaggagca ggtgttccgt ttgagatcgt caatgtatcg attgctttga ttatcttctt
    25081 tgtcgggatc aacttcctga ttcgtttcgt catggctaaa gtcttccaag ggaaaaaaca
    25141 agaagagatc gttgcgacaa tagaatcgaa accaacgaat caaaaccatg aagggggcga
    25201 actataatgt cgactatcga attaattgcc ttgatcctta cttctacact ggtttattca
    25261 acaccgctaa ttctaacgtc actaggcgga actttttctg aacgaagcgg gatcgtaaac
    25321 gtagggctag agggaatcat ggtcatgggt gcttttagtt ccatcgtttt caacttgacg
    25381 atgagtccat cacttggttc tatgacacca tggatcgcta ttttagtggg tggcgtcgta
    25441 ggtatcctat tttctctgat tcacgcagta gcaacagtca gtttgcgtgc tgaccatatt
    25501 atcagcggga cagtaattaa tctgatggca cctgctttag gtgttttctt gatcaaagca
    25561 tggtatggta aaggtcaaac tgataacatc acgcagaatc tggggtattt ttctttccca
    25621 ggattagcag atattccggt cattggacca attttcttta aaaatacatt cttacctgct
    25681 tattgtgcta ttttgattgc tgtcctagct tgggtgatca tcttcaagac gaaattcggc
    25741 ttgcgcctcc gttcagttgg tgaaaatcct caagctgccg acacattagg tatcaatgta
    25801 tatgctatgc gttattcagg cgtactgatt tctggcttgt taggcggtat gggcggtgct
    25861 gtttttgctc agtcgatttc cggtaacttt tcagctggta cgatcgttgg acaagggttc
    25921 atctccatgg ctgctatgat ctttggtaag tggaatcctt taggggcaat gggtgcctca
    25981 ttgttctttg gttttgctca aagcttgagt atcattggcg cacaattacc agttatttct
    26041 tcgattccgc cagtattgct gcaaattgct ccatatttgc tgacaatcat tgtgctggtt
    26101 gttttcttag ggaaagcttc tggtccaaaa gcaaacggga agaactatat caaatcaaaa
    26161 taatctttta gcgaataaaa agggtgggtt acgggaagtg tttttcgtct cgcccttttt
    26221 gttttaaagg caataaaaat aaaaagaaag aaggaataat catgtttaaa cgcgtacatc
    26281 tgatcgtcat ggattcagta ggaattggag aagcaccaga tgccgataag ttcggtgatg
    26341 ttggttctga tacattagga cacattgcga aagaagcagg attgaacgtt ccacatttag
    26401 agcagcttgg tttaggaacg atccgtcctc ttgaaggtgt ggaatcagtg aaagatcatc
    26461 aaggctatgc gacgaaatta gaagaagttt ctgtagggaa agatacgatg acaggccact
    26521 gggaaatcat gggattgaat atcaaaactc catttcgtgt gtttcctaat ggattccctg
    26581 atgatctgat caaacaaatc gaagaatttt ctggccgtaa agtcgtatgt aacaaacctt
    26641 atagcggaac agctgttatt gacgattacg gtgaacacca aatgaaaaca ggcgatttaa
    26701 ttgtatatac ttctgccgat cctgtccttc agatcgcggc acatgaagaa atcattcctt
    26761 tagaagaatt atatcggatt tgccaattcg ttcgtgatat aacaaaagat gatccgtata
    26821 tgattggccg aatcattgca cgtccgtacg tgggtgaacc aggagacttc acacgaacaa
    26881 gcaatcgcca tgattatgcg ttgaatccat tcggacacac agtccttgat tctttgaaag
    26941 aagcaggaaa ggacgtgatt gctgttggaa agatcaacga tattttcaac ggacaaggaa
    27001 tcactgaatc tgtccgcacg aaaagcaata tggatggcgt tgaccagctg ctgaaagtta
    27061 tgaaaaacga tttttcagga atcagcttta ccaatttagt agattttgat gcattatacg
    27121 gtcatcgtcg ggatgtagtc ggctatgcgc atgcgattga agaatttgac ttacgaattc
    27181 cagaattgct tgaagcgatg gaagaagacg acttgctaat gattacagcc gatcacggaa
    27241 acgatccgac gtttactggt accgatcata cgcgagaata tgtaccgtta ttggtttaca
    27301 gtaaaaaaat gaaagaacaa ggggcattgc ctcaaggata ttattcagat atttcagcaa
    27361 cgattgctga gaactttagt gtttcttcga ctgaaaatgg cgaaagcttc ttgaaattat
    27421 taaaataatc tttttaaata ggagaaagca aatgaccaag ttaagcgaga tgttacaaga
    27481 aacgacccag tttatccttg ataaaggagt aaaagaaatt gattttggtt tgattctagg
    27541 ttcaggatta ggtgaattag cagatgaaat cgaagatgct gtaatgattt cttatgatga
    27601 gatcccgttc tttcctgttt cgacagtagt tgggcatgca ggaaagctag tttatgggtc
    27661 tctggcaggc aagaaagtgt tagccatgca agggagattc cactactatg aaggtcattc
    27721 catgcaaaca gtgacctatc cagtacgtgt gatggccgga ctaggggccc attcagtcat
    27781 tgtgactaat gcatgtggtg gtgtcaacga atcatttgta ccaggagatt tgatgctgat
    27841 taccgatcat atcaatttta ctggtgaaaa tcctttgatc ggtccgaatg aagatgcaat
    27901 gggccctaga tttccagata tgagtcaagc ttatacgcct gactatcaag caacagcaaa
    27961 agcagcagca gaaaaactag gtttggatct taaagaaggc gtatatatgg gctattctgg
    28021 accaacttat gaaacgcctg cggagatccg aatgtctcgg acaataggag ccgatgcagt
    28081 agggatgtcg actgttccag aagtcatcgt agcagctcac agcggattaa aagtgttagg
    28141 aatctcttgt attaccaact tagcagcagg tatgcaagcg aatctgagcc atgaagaagt
    28201 tgtagaaaca actcagagag tcaaacaatc ttttaaagca ttggttaaag aaacattgac
    28261 attattgtaa taggaggaac aataaaatga gtgtgcatat tgaagcgaaa gaaggcgaga
    28321 tcgccgataa gattttactt ccgggagatc ctttgcgagc taaatatatt gcggagactt
    28381 ttttggaaga tccagtttgt tacaatcagg tacgcggtat gttaggcttt accggcaaat
    28441 ataaaggaga acgtatttct gttcaaggaa caggaatggg aatgccatca gccaccatct
    28501 atgcccatga attgatccag tcgtatggtg taaagaaatt gattcgagta ggtacatgcg
    28561 gtgcattgtc aaaagacgtc catgttcgtg atttagtact agcacaaggt gcagcgacca
    28621 gttcttcgat gatcgaaaaa aatttccaag cctttcattt tcctccaatc agtgatttca
    28681 atctattact gaaagcatat gagatagcta aagaaaaggg ctatacggtt catgtaggaa
    28741 atgttctttc ggaagattct ttctacaaag atgatttgac agagacgttc caattagctg
    28801 aacttggcgt tttaggagtg gaaatggaag cagcggctct ttattactta ggcgcgaaat
    28861 accatgtgca gacattaagt ttgatgaccg ttagcgacca tttgatcacc ggtgaagaaa
    28921 caacagctgc agaacgtcaa tctacgttca atgaaatgat cgaagtagga ttggaaacag
    28981 cgatagcaga ataagaagga aatgaaaaag ctgccctgca ttaagtaata agaacatcca
    29041 agcaaaaata gctcctcatg tttttgactt ggctggactt attattgcaa atgtaagctt
    29101 ttgaacaccg tttaccaaga ggttgtgaat ctgccgttta actctgagtc acaaggaaca
    29161 atttcaaaaa acagcttttc atgtttgatg aatattgtga cttgtgaccg agaagttggc
    29221 aggagaaaac cgtttatacg aaaaaaggga ctgtgacaaa tcgtgtcaca gtcccttttt
    29281 tatagtgttt tttataggta gtatttttta gcaactgtta agtcatcatt taattcataa
    29341 acaagtggtt gacctgttgg aatttcaaga tccatgatgt cttcgtcaga gataccttcg
    29401 atatgtttag ctagggcgcg taatgagtta ccatgtgcag ctactaaaac agttttgttg
    29461 tctagtaatg ctggagcaat ttcatcttgc cagaatggca aagcgcgttc taatgtcact
    29521 ttcaagtttt ctccaccagg aatatcgcgt ttgtctaaca ttgcgtaacg acggtcattt
    29581 gctgcagaac cttcgtcagt ttcttccatt aatggaggaa gagtatcgta agaacgacgc
    29641 cagatgtgca cttgttcgtc gccgtatttt tcagctgttt cttttttgtt caaaccttgt
    29701 aatttaccat agtgacgttc gtttaagcgc catgatttga tttgtggtac ccaaagttga
    29761 tctgaatatt ctaagatcaa gttacatgtt ttgattgcac gagttaaaac agaagtgtaa
    29821 gcaacatcaa attcgattcc agcttctttg attttgcgtc cgccttcttt tgcttcttcg
    29881 attccttctg gtgctagatt tacatcagcc caaccagtaa attggttcaa tgcattccat
    29941 tcactaagac cgtgacgaga aaaaactaat tttggcatgt ttagctctcc ttttaagata
    30001 ggtttatatt tctgaaattt tcagttctgt tttattgtac aattttttgg aagagatttc
    30061 catcgaaaaa ttagataaga ttctatgaga aaacaagtag agaatataaa aaaacgaaca
    30121 gtgcccacgc aactgttcgt ttttaggtat tagatgcata attgctcgat ttctttttca
    30181 ggtaaattga tatatttata acatgttcca actgaaacac cgcattttga agaaataaat
    30241 tggatcgttt cttttttttc ataatagaga cgtcggattt ttttgatcgt ttttgcatcg
    30301 atttttggtc ttccgccgat ttttcctttt ttacgagctt catctaatcc aactaatgta
    30361 cgttctttga tcaatgcgca ctccatgttt gctaggtttt ccattaattg gaaatagatt
    30421 tttcccattt cgcgcgtatc gattccttca tcaagactaa tcaagtgcag cccgcgatct
    30481 ttgaataatt gcgtgagttc tgttaattgc cgcgtagatt tgcctaatcg atgaagttgg
    30541 caaatgacta gtgcatcgcc ttgttgcatg tcagcgatca ctgactccaa atgaacagca
    30601 gtagaatcat tttgttccac ttcatatgtc tcatggaaaa tttcttgaca accaaagtca
    30661 gataattgat caacctgagt tgttaaatca acgtcattta tggttgtgcg tgcataacca
    30721 ataattttca tggtgattcc tttctagcag tgattttcta actggatata acagtaaaaa
    30781 tgtagcataa agtacgtcaa aaagatagtt ttttcgcaaa ttttccgctg tcttttttta
    30841 ttggatgatc aagcagtttt tctttattta cgacggattg cgtcaagaat ctcaacgcct
    30901 ttttctgagc cgacaactac tttagaaacc atgtttagga aaagcccatg ttctactacc
    30961 ccaacaagtt catctaaata ggtagctaag ctttctggct gttcgatgac ttctagatgc
    31021 agatcaatga tgtaatgacc accatctgtg gttaagactt ctccgttttc gtctgtacgc
    31081 aagacgggtt ggaatctttt ttcttcaaaa atatgaagta gctgttggga gccataaggg
    31141 atcacttcaa ctgggagtgg gaacttccca agtttatcga ctaatttaga agaatcaacg
    31201 atccaaatcg tttcttttga gtacgtagcg actattttct cgaaaagcaa agcagcgccg
    31261 ccgcctttga tcccttgaaa atcagcactg atttcatcag caccgtctat tgtcaaatcg
    31321 actacgggga cttcatcaat actctttaac ggtattccta atttttccgc ttgttctttt
    31381 gtcgcatttg aagtagtaac tcctgtgata cgtaaacctt cttcacgcat acggcgacca
    31441 atctcttcta ccatataata ggcggttgaa ccggtcccga gacctacgat catcccgtcc
    31501 tttacgaatt tcgcggcttc aatgccagct aattgtttta aattcatctt tgcgtccacc
    31561 tttttttagt ttgagcatcg tgctttcctc actattttag cgtgatttac ggtaaaaaga
    31621 taccattctt ttttattcca ataaagcttt ttcaaaaaaa attgcatctc ttgttgacat
    31681 gttttttcat tcgtgatatt ctaacaaagg tgctttatct acaggtttct gtgaagaaac
    31741 gtgctgactg tttatcaaag catatttgat aaggatgcag acattgcatt tattttttat
    31801 cacaaaaaaa gaaccacctg gatgtgtgga actagacgca aatcgtggga aggaggaaac
    31861 aaggaatgcc tacaattaac caattagtac gtaaacctcg taaatcaaag gtggaaaaat
    31921 ctaattcacc tgctttgaac aaaggatata acagctttaa gaaaactcaa actaacgtaa
    31981 actcacctca aaaacgtggg gtagcaactc gtgtgggtac aatgacacct aaaaaaccga
    32041 actcagcttt acgtaaatat gcacgtgttc gtttgtcaaa cttaattgaa gttactgctt
    32101 atatcccagg gattggtcat aacttacaag aacacagtgt tgtattatta cgcggtggtc
    32161 gtgtaaaaga cttaccaggg gtacgttacc atatcgttcg cggtgcttta gatactgctg
    32221 gtgttaacga ccgtaaacaa agccgttcta aatacggaac taaaaaacct aaagcttaat
    32281 tttaattatt aaacaaatga aacttgcttc tactatatag aagtaattac tatgaacaat
    32341 atttcggaag gaggagttac ggatgccacg taaaggtcct gttgcaaaac gtgatgtttt
    32401 accagatcct atttataact caaaattagt aactcgcttg atcaaccgtg taatggttga
    32461 cggaaaacgc gggattgcag ctaacattat ctataatgcc tttgacttga tcaaagaatc
    32521 tacaggtaac gatccattag aagtgtttga acaagcaatg aaaaacgttc aacctgttct
    32581 tgaagttaaa gcacgtcgtg ttgggggttc aaactaccaa gtaccagttg aagttcgccc
    32641 tgaacgccgc gttactctag gtcttcgttg ggtagtaaac tacgctcgct tgcgcggtga
    32701 acacacaatg gaagaacgct tagcaaaaga aatcatggat gctgcaaaca acacaggtgc
    32761 ttctgttaaa aaacgtgaag acactcataa aatggctgat gccaaccgtg cgtttgctca
    32821 ctatcgctgg taagattctc tctgtttggt cggcttttat aagtcgacct cagatagtgt
    32881 ccttattttt agcaactaga tagtaaagag aggagaaaca aggagaatga ccagagaatt
    32941 ttctttacaa aacactcgta atatcggtat catggcccac atcgatgctg gtaaaacgac
    33001 tgctactgaa cgtatcttgt actatactgg taaaatccat aaaattggtg aaactcatga
    33061 aggagcttca caaatggact ggatggaaca agaacaagaa cgtggtatta cgatcacttc
    33121 tgctgctact acagcacaat ggaagggtca ccgaatcaac atcatcgaca caccaggaca
    33181 cgtagacttc acagttgaag ttgaacgttc attgcgtgta cttgatggtg ctgtaactgt
    33241 tcttgatgca caatcaggtg ttgaacctca aactgaaaca gtttggcgtc aagcaacaac
    33301 ttatggtgta cctcgtatcg tatttgcaaa caaaatggac aagatcggtg ctgacttctt
    33361 atattcagta tcaactttac atgaccgttt gcaagcaaac gctcacccaa tccaattacc
    33421 aattggtgcg gaagacgact tcacaggaat tatcgacctt gtaacaatga aggctgaaat
    33481 gtatacaaac gatcttggaa cagagatcga agaaacagaa attcctgaag aataccgcga
    33541 attagctgaa gaatggcgcg aaaaattggt tgaagctgta gctgaaactg atgaagaact
    33601 tactttgaaa tacttagaag gtgaagaaat cactgaagct gagttaaaag aaggtatccg
    33661 ccgcgcaaca gttaacgtag aattctaccc agtactatgt ggttcagcat ttaagaacaa
    33721 aggggttcaa ttactattgg acgctgttct tgattacttg ccatcaccgt tagatatccc
    33781 agctatcaaa gggattgacc ctaaaactga tgaagaagta gaacgtcctg cagacgacag
    33841 cgctcctttc tcagctttag catttaaagt tatgactgac ccattcgtag gtcgtttaac
    33901 tttcttccgt gtgtactctg gtgttttaca atcaggttca tacgtacaaa acgcaacaaa
    33961 aggcaaacgt gaacgtgttg gtcgtatcct acagatgcat gccaactctc gttcagaaat
    34021 ctctgaagtt tacgctggag atatcgctgc tgctgttggt ttgaaagaca caactactgg
    34081 ggacacacta tgtgatgaaa agagtctagt tattcttgaa tccatggaat tcccagaacc
    34141 agttatccaa gtcgctattg aacctaaatc aaaagctgac caagataaaa tgggtgtggc
    34201 tttacaaaaa ctatctgaag aagatcctac tttccgtgct gaaacaaacg tggaaacagg
    34261 cgaaacaatc atcgctggta tgggtgaact tcacttagat atcatcgttg accgtatgcg
    34321 tcgtgaattc aaagttgaag ctaacgtagg tgcgcctcaa gtatcttacc gtgaaacttt
    34381 ccgtgctggc actcaagccg aaggtaagtt tgtacgtcag tctggtggta aaggtcaata
    34441 cggtcacgta tggattgaat ttacaccaaa tgaagaaggt gctggtttcg aatttgaaaa
    34501 tgcgatcgtc ggtggggttg ttcctcgtga atacattcca gctgttgaaa ctggtttgaa
    34561 agatgctatg gaaaatggtg tcttagctgg ctatccatta gtagatatca aagctaaact
    34621 ttacgatggt tcataccatg atgtcgattc aaatgaaact gccttccgtg ttgctgcttc
    34681 tatggcatta cgtgcagcag ctaagaaagc taatccagta atccttgaac caatcatggc
    34741 tgtagaagtt gttatccctg aagattattt aggtgatgtt atgggtcacg taactgctcg
    34801 tcgtggacgt gttgaaggta tggaagctcg cgctggcggc caacaagttg ttcgtgcgat
    34861 ggttccactt gctgaaatgt ttggttatgc tacaactctt cgttctgcta ctcaaggtcg
    34921 tggtacattt acaatgacat ttgaccacta cgaagatgta ccaaaatcag tacaagaaga
    34981 aatcattaag aaaaacggcg gaaaagctga ataatttctt aaaaccggat agtcctgcaa
    35041 agagattatc caaagagact atatttttaa tatagtttcg tgtaaaataa ttatcgatga
    35101 tatgggcatg tggtgtacct tgtgtacatc atatacctat cagacaaata aaaaatatat
    35161 tgtattttta ggaggacatt ttaaaatggc aaaagaaaaa tttgaccgtt ctaaaccaca
    35221 cgttaacatt ggtactatcg gacacgttga ccatggtaaa actacattaa cagctgcaat
    35281 cacaactgta ctatctaaga aaaacggcgg ccaagctatg gcttacgatc aaatcgatgg
    35341 tgctccagaa gaacgcgaac gcggaatcac aatctcaaca gctcacgttg agtatgaaac
    35401 agatacacgc cactacgctc acgttgactg cccaggacac gcggattacg ttaaaaacat
    35461 gatcactggt gctgctcaaa tggacggagc tatcttggta gtttctgctg ctgacggccc
    35521 aatgcctcaa actcgtgaac acatcctatt gtctcgtcaa gttggtgttc cttacatcgt
    35581 tgtattcttg aacaaagtag acatggttga tgacgaagaa ttactagaat tagttgaaat
    35641 ggaagttcgt gacctattaa cagaatacga attccctggt gacgatgttc ctgtagttgc
    35701 tggatcagct ttgaaagctc tagaaggcga cgcttcatac gaagaaaaaa ttcttgaatt
    35761 aatggctgca gttgacgaat acatcccaac tccagaacgt gacaacgaca aaccattcat
    35821 gatgccagtt gaagacgtgt tctcaattac tggacgtggt actgttgcta caggtcgtgt
    35881 tgaacgtgga caagttcgcg ttggtgacga agttgaagtt gttggtattg ctgaagaaac
    35941 ttcaaaaaca acagttactg gtgttgaaat gttccgtaaa ttgttagact acgctgaagc
    36001 tggagacaac attggtgctt tactacgtgg tgttgcacgt gaagacatcc aacgtggaca
    36061 agttttagct aaaccaggta caatcacacc tcatacaaaa ttctctgcag aagtatacgt
    36121 gttgacaaaa gaagaaggtg gacgtcatac tccattcttc actaactacc gtccacaatt
    36181 ctacttccgt acaactgacg taacaggtgt tgttgaatta ccagaaggaa ctgaaatggt
    36241 tatgcctggc gacaacgtaa caatggaagt tgaattaatc cacccaatcg ctatcgaaaa
    36301 cggtactaaa ttctcaatcc gtgaaggcgg acgtacagtt ggtgccggtg tcgttacaga
    36361 aatcaaagct taattcactt aagttttaaa gtaaagcaac atttattcgg acgtaatcgc
    36421 tcggaacgaa ggtcttagga tctttgttcc gagctttttt atttattgca ggatcaggaa
    36481 aaaagacagg agtattataa atagaagaaa cacgaaataa aagagtcagt attttctaaa
    36541 tgaaacttta tgaaaacaaa atgtttttta caagaaaaag ccttgctttg tgagtttata
    36601 tatattataa tgacaaaggt gcgtttactt tagtgaattg cacagacgta atcagaatga
    36661 tttcgtcggc taagaaacga gaggttgcga cacgcccgga cgctttgcca cgaacgagcg
    36721 tgacggaaat tttcgtggag ctatgtctac ttttcaaaat aggcgaagga gggaacaaga
    36781 tggcaaaaca aaaaattcgt atccgtttaa aagcgtatga acaccgtatt ttagatcaat
    36841 cagcggataa aatcgtggaa actgcaaaaa gaactggagc tagcgtgtct ggtccaattc
    36901 cattaccaac tgaacgcagt ctttacacaa tcatccgtgc gactcatgaa tacaaagatt
    36961 cacgcgaaca attcgaaatg cgtacacaca aacgtcttat cgacattgtg aacccaacac
    37021 caaaaactgt tgatgctttg atgaagctag acttaccatc tggtgtaaac atcgaaatca
    37081 aattataaaa ctaaaacaaa aataatggag gtgtactcat gaccaaagga atcttaggga
    37141 aaaaagtggg aatgacacaa atcttcactg aatctggtga attaattcca gtaactgttg
    37201 tagaagctac tccaaacgta gtactacaag tgaaaacaat ggaaacggat ggctacgaag
    37261 ctattcaagt tggttaccaa gatatgcgtg aagttttatc aaacaaacct gcgaaaggtc
    37321 atgttgcaaa agcaaacacg gctcctaagc gcttcattcg tgaattcaaa aatgttgagc
    37381 taggagaata tgaagtagga aaagaaatta cagtagatgt tttccaagca ggagacatta
    37441 ttgatgtaac aggtactacg aaaggtaaag gattccaagg ggttatcaaa cgccacggac
    37501 aaagccgcgg accaatggct cacggttctc gttaccatcg tcgtcctggg tcaatgggtc
    37561 cagttgcacc taaccgtgta tttaaaaaca aacgtcttgc tggccgtatg ggtggcaacc
    37621 gcgtaactat ccaaaacttg gaagttgtac gtgtcgatgc agaaagaaat gttatcttga
    37681 tcaaaggtaa tgttcctgga gctaaaaaat ctttaattac tatcaaatca gctgtgaaag
    37741 ctaaataatt gtaggaagaa aggaggaact aaggaatgcc gaatgtagca ttattcaaac
    37801 aagatggaag ccaaaacggt gaaatcacac ttaacgaaga aatcttcgga attgaaccta
    37861 atgaaagtgt tgtttacgat gcaatcgtaa tgcaacgtgc gtcattaaga caaggaacac
    37921 acgcggttaa aaaccgtagc gctgttcgcg gcggcggacg taaaccatgg cgtcaaaaag
    37981 gaacaggtcg tgcacgtcaa ggatctatcc gctcaccaca atggcgtgga ggtggcgtag
    38041 tctttggacc aacaccacgt tcttacagct acaaacttcc taaaaaagtt cgtcgtttag
    38101 caatgaaatc tgtattgtca gaaaaagttg ctgaaaacaa cttggtagca atcgaaggct
    38161 tgaacttcga cgcaccaaaa acaaaagaat tcaaacaagt tcttgctaac ttatctatcg
    38221 actctaaagt attggttgtt ctagaaaaag gtaacgactt tgctgcttta tcagcacgta
    38281 acttaccaaa cgtttctgta gtaacttctg ataatgtgag tgtgctagac gttgtatcaa
    38341 atacaaaagt gttagcaaca caaactgctc ttactcaaat tgaggaggtg cttgcataat
    38401 gaacttacta gacgtaatca aacgcccagt gatcactgaa aaatcaatgc ttgccatgga
    38461 tgacaagaaa tacacttttg aagtggacac tcgcgcaaac aaaactttag taaaacaagc
    38521 ggtagaagct gcttttgacg ttaaagtcaa aaacgtgaac atcgtgaacg tacgtgcgaa
    38581 attcaaacgt atgggcaaat atgcaggata tacaaaaaaa cgtcgcaaag cgatcgttac
    38641 attgactgaa gattcaaaag aaatccaatt attcgaagct gctgaataag cactaataga
    38701 taactcatta aattaggagg gaaaaacgtg gcgattaaaa agtacaaacc tacctcaaat
    38761 ggtcgtcgta acatgacagg ttctgatttt gctgaaatca caacatcaac acctgaaaaa
    38821 tcattgttac aaccattgaa aaaccatgct ggacgtaaca acaacggccg catcactgta
    38881 cgtcatcaag gtggcggaca caaacgccaa tatcgcgtga tcgacttcaa acgtaacaaa
    38941 gataacgttg ttgcaactgt tcaaacgatc gaatatgatc caaaccgttc agctaacatc
    39001 gcgttagtac actatgaaga tggagtgaaa gcttacatct tagcacctaa aggactagaa
    39061 gtgggtatga aacttgtttc aggtccagaa gcagatatca aagtcggaaa tgctcttcca
    39121 ttagaaaaca ttccagttgg tactgttatc cacaacattg aaatgaaacc tggtaaaggt
    39181 ggacaattga tccgttctgc tggtacaagt gctcaagtac ttggtaaaga aggcaaatac
    39241 gtattgatcc gcttgaactc tggtgaagtt cgtatgatct tagctgcatg ccgtgctaca
    39301 atcggttctg taggtaacga acaacacgaa ttaatcaaca tcggtaaagc tggacgctct
    39361 cgttggatgc gtaaacgccc aactgtacgt ggtagcgtaa tgaaccctaa tgatcaccca
    39421 cacggtggtg gtgaaggtaa agctcctatc ggacgtaaag caccagtttc tccttggggt
    39481 caaccagctc ttggttacaa aactcgtaac aaaaaagcta aatcagacaa acttattgtt
    39541 cgtcgtcgca aaaataaata attactttga ccatgtgtcg cacattttga gaggaggttc
    39601 accatgggtc gtagtttaaa gaaaggacct tttgtcgatg agcatttaat gaaaaaagtt
    39661 gaagctcaag caggggttga aaagaaaaaa gttatcaaaa cttggtcacg ccgttcaaca
    39721 gttttcccaa gttttgttgg atataccatc gcagtttatg atggacgtaa acatgtacca
    39781 gtttacatcc aagaagacat ggtaggacac aaattaggtg aatttgcacc tacaagaact
    39841 tatcgtggac atgctgctga cgataagaaa acgaaacgct aatttgaggg gaggataaat
    39901 ccaaatggca gaacaaatca catcagctaa agcaactgca aaaacagttc gcacttcacc
    39961 tcgaaaagcc cgtttagtaa ttgaccttat cagaggtaaa agtgtcgcag atgcaatttc
    40021 aatcttgaaa ttcacaccaa acaaatctgc tggaatcatt gaaaaagtat tgatgtcagc
    40081 agttgcgaat gcagaaaata actttgactt agatgttgaa aacttggtag taagcgaagc
    40141 ttttgttaac gaaggaccta caatgaaacg tttccgtcct cgtgcaaaag gatcagcttc
    40201 tccaatcaac aaacgtacaa gtcatatcac ggtagtcgta tcagaaaaat aaggagggat
    40261 aatcagtggg tcaaaaagta catccaattg gaatgcgtgt aggcatcatc cgcgactggg
    40321 atgctaaatg gtatgctgaa aaagagtatg ctgagttctt acacgaagat ttaagaatcc
    40381 gtaaatttat ttcaactaaa cttgctgatg ctgctgtatc aacagttgaa atcgaacgcg
    40441 caacaggtcg cgtaaacatt tcaattcaca cagctaaacc aggtatggtt attggtaaag
    40501 gcggttctga agtcgaaagc ctaagaaaag aattaaacaa attaactggt aaaagaatcc
    40561 atatcaatat cgtggaaatc aaaaaaccag atctagatgc aaaattagta ggtgaaggaa
    40621 tcgcacgtca attagaaaac cgtgttgctt tccgtcgcgc tcaaaaacaa gccatccaac
    40681 gctcaatgcg tgctggcgct caagggatca aaactcaagt atctggtcgt ctaaacggag
    40741 cagatatcgc tcgttcagaa ggatactcag aaggaactgt tcctcttcat actttacgtg
    40801 cggacattga ttacgcatgg gaagaagcag atacaacata cggaaaacta ggagttaaag
    40861 tgtggatcta tcgtggagaa attcttccaa ctaaaaaaaa cactgagaaa ggagggaaat
    40921 aatcatgtta gtacctaaac gtgtaaaaca ccgtcgtgaa ttccgcggaa aaatgcgcgg
    40981 ggaagctaaa ggcggaaaag aagtagcatt cggtgaatac ggtttgcaag ctgttgattc
    41041 acattggatc acaaaccgcc aaatcgaagc tgctcgtatc gcaatgactc gttacatgaa
    41101 acgtggtggg aaagtatgga ttaaaatttt ccctcacaaa tcttatactg ccaaagcaat
    41161 tggggtacgt atgggttctg gtaaaggggc acctgaagga tgggttgcac cagtaaaacg
    41221 tggtaaaatc atgtttgaaa tcgcaggcgt tcctgaagaa gtagctcgtg aagcgttacg
    41281 tctagcttct cacaaattac caatgaaaac taagatcgta aaacgtgagg aaatgggtgg
    41341 tgaatcgaat gaaggttaaa gaaatcagag aattaaccac tgccgaaatg cttgatcaag
    41401 aaaaacaatt aaaagaagaa ttgtttaatc ttagattcca attagcaaca ggtcaattag
    41461 aaaacactgc acgtattaaa gaagtacgta aatcgattgc acgcatcaaa actgtgttgc
    41521 gtgaacaagc taagtaatta tggaaggagg ccattaagcg tatgactgaa ggaagaaatc
    41581 aacgcaaagt ttatcaaggt cgcgtggttt cagacaaaat ggataaaaca attaccgttg
    41641 ctgtcgaaac aaagaaaaac caccctatct atggcaaacg catgaactat tctaagaaat
    41701 acaaagtgca tgatgaaaac aacacagcaa aagttggcga catcgtgaga attatggaaa
    41761 ctcgtccatt atcagctaca aaacgtttcc gtttgttgga agtagtcgaa gaagcagtta
    41821 ttatctaata acacgagatt ttcctatata gattgaatgc aaaatctgaa aggaggatac
    41881 acaacgtgat ccaagcagaa agtcgtttaa aagtcgctga taactcaggc gcacgtgaaa
    41941 ttttgacgat caaagtcctt ggtggatctg gtcgtaaaac tgctaacatc ggtgatgtga
    42001 tcgttgctac ggttaaacaa gcaacgccag gtggggttgt caaaaaaggt gacgtcgtta
    42061 aagccgttat cgttcgaaca aaatcaggag ctcgtcgtac agacggttca tatattaaat
    42121 ttgatgaaaa tgctgcagtg attattcgtg atgataaaag ccctcgtgga acacgtatct
    42181 ttgggccagt tgcacgtgaa ctacgtgaaa acaacttcat gaagatcgtt tcactagcac
    42241 cagaagtttt ataatcttga gacacttatc aaaggaggtg cgaaacagtt atgtttgtta
    42301 aaaaaggcga taaagttaaa gttatcactg gaaaagacaa aaacaaagaa ggcgttgtac
    42361 tagcagcgtt cccgaaaaaa gataaagtta ttgtcgaagg tgtaaatgtc gttaaaaaac
    42421 accaaaaacc ttctcaagct gctcctcaag gtggaattgt tgagatggaa gcgccaattc
    42481 atgtttctaa cgtgatggtg gttgactcta ctggtgtagc tggccgagtt ggctacaaag
    42541 aagtagacgg caaaaaagtc cgtgtttcta aaaaaaccgg tgaagtttta gataaataaa
    42601 agtttaagga aggagggtct tactgaatga accgcctaaa agaaaaatat cttaaagaag
    42661 taactccatc attgatggaa aaatttaact atagctctgt tatgcaaaca cctaaagttg
    42721 aaaaaatcgt catcaacatg ggtgttggtg atgcggtatc aaacgctaaa aacttagata
    42781 aagcagttga agaattaact ttgatcactg gtcaaaaacc aatgattaca aaagctaaaa
    42841 aatcaatcgc tggattccgt ttacgtgaag gaatgccaat tggtgcaaaa gttacattgc
    42901 gcggagaaag aatgtacgaa ttcttagaca aattggtttc tgtttcacta ccacgtgtac
    42961 gtgacttcca tggtgtaagt aaaaaagcct ttgacggtcg tggaaactat actttaggta
    43021 ttaaagaaca attgatcttc ccagaagtag attatgattt agtagataaa gtacgtggta
    43081 tggacatcgt tattgtaaca acagcaaact ctgatgaaga atctcgtgag ttgttgacac
    43141 aattaggcat gccattccaa aaataattaa aggaggcgaa ctacgtggct aaaaaatcaa
    43201 tgattgctaa aaacaaacgt cctgcaaaat tttcaacaca agcttatact cgttgcgaac
    43261 gttgcggacg tccacattca gtttatcgta agttccatct ttgccgtatt tgcttccgcg
    43321 aacttgccta taaaggtcaa attcccggcg tgaagaaagc tagctggtaa aaagtaaatt
    43381 cgcataagaa ggaggtaaaa gatcaatggt catgacagat ccaattgcag attttctaac
    43441 tcgtatccgt aacgcaaaca tggttaaaca tgagtcttta gaagtacctg catcaaaaat
    43501 taaacgtgac atcgctgaaa tcttgaaacg tgaaggtttc gttcgtgatg ttgaatacat
    43561 cgaagatgac aaacaaggcg tgatccgtgt tttcctaaaa tttggtaaaa acggcgaacg
    43621 tgttatcaca aacttaaaac gtatctctaa accaggtttg cgtgcttatg tcaaagctga
    43681 cgaagtacca aaagtattga acggtttagg tatcgcaatc atttcgactt ctgaaggtgt
    43741 tgtcactgat aaagaagctc gtgaaaaaaa tatcggcggc gaagttatcg cttacgtatg
    43801 gtaatcaagt agaatctaca aggaggtgtc tttaagtgag ccgtatcgga aataaagtcg
    43861 ttgttatccc tgaaggagta acagttactc aagacggaaa caacgttaca gttaaaggac
    43921 caaaaggtga attgactcgt actttttcag cagatatcaa aatgaatatc gaaggaaacg
    43981 aagtaacttt cactcgtcca aacgacagta aagaaatgaa aacaattcat ggaacaactc
    44041 gtgcgaactt caacaacatg gttgtaggtg tcagcgaagg tttccaaaaa gcgttagaac
    44101 taatcggggt tgggtaccgt gcacaattac aaggatcaaa acttgtattg aacgttggtt
    44161 actcacatcc agtagaaatc acaccaccag ctggagtaac tgttgaagta ccagctaaca
    44221 cacaagtaat tgttaaaggt gcgaacaaag aagaagttgg cgaattagct gcaaacattc
    44281 gtggaactcg tcctccagaa ccttataaag gtaaaggtat tcgttatgtt ggcgaatatg
    44341 tacgccgtaa agaaggtaaa actggtaaat aatcgtttag aaaaattgca tagaagccag
    44401 cagcaggaaa aaggcatttg gctgaagttt accttcaaaa aaatacattt ttacttgtgc
    44461 cgcagcttct atttttcgaa acgtatttac tgcttgaaat agtagtacaa taaaaataaa
    44521 gaggtgacaa ttgtgattac aaaaccagat aagaacaaaa cacgtcaaaa gagacatcgt
    44581 cgtgtacgta acaatatctc tggtactgct gagcgcccac gcttgaacgt ttttcgttct
    44641 aacaaaaaca tctacgctca aattattgat gacgtagcgg gtgtaacgct agcaagtgcc
    44701 tctaccttgg ataaagaaat ttcaggtgga acaaaaacag aagcagcaca agctgtcggt
    44761 aaactagttg ctgaacgtgc agctgaaaaa ggcattaaag tagtagtctt tgaccgtggt
    44821 ggataccttt accatggccg tgtgcaagct ttagctgaag ctgctcgcga aaatggacta
    44881 gaattttaga agaaggagga ataccattca tggtttatat cgatccaaaa catttggaat
    44941 tagaagaccg cgttgttgca attaaccgtg taacaaaagt tgttaaaggt ggacgtcgtc
    45001 tacgttttgc tgctttagtt gttgtcggtg acaaaaacgg acacgtaggg tttggtacag
    45061 gtaaagctca agaagttcct gaagcaatcc gtaaagcaat tgaagatgcg aagaaaaact
    45121 tagttgaagt acctatggtt ggttcaacaa tcccacacga agtaatcggt gcttttagtg
    45181 gtggtagaat cctaatgaaa cctgctgtag aaggttctgg ggttgctgct ggtggaccag
    45241 ttcgtgccgt attagaatta gctggggtag cagatatcac ttctaaatct ttaggctcaa
    45301 atacaccaat caacgttgtt cgcgcaacag ttgaaggttt gaaacaattg aaacgtgcag
    45361 aagaagttgc agaacttcgc ggcaaatcag ttgaagaatt aatcggataa ggaggacaac
    45421 aataatggct gaattaaaag ttactttaaa acgcagtatt atcggacgtc ctcaaaacca
    45481 acgcgataca gttaaagcgt taggtcttac aaagattaac agttcagtgg ttaagccttc
    45541 taatgaagca attaaaggca tgatcaatac tgtttctcac ctagtggacg ttgaagaaat
    45601 ttaatttaga aaaatcataa ctagaacatt aaggaggtgc caaacctatg aaacttcatg
    45661 aattaaaacc tgctgaaggt tcacgccaag tacgtaaccg tgttggtcgt ggtacttcat
    45721 ctggtaacgg aaaaactgcc ggacgcggac aaaaaggaca aaaagctcgt tcaggcggcg
    45781 gtgtgcgttt aggatttgaa gggggacaaa caccattgtt ccgtcgttta ccaaaacgag
    45841 gattcacaaa cgtgaaccgt aaagactatg cagtcattaa tcttgatgta ttgaaccgct
    45901 ttgaagacgg agctgaagta actcctgttg ctttagttga agctggaatc gtgaaaaacg
    45961 aaaaagctgg aatcaaagta ttagctaatg gggaactaaa caagaaatta actgtgaaag
    46021 cagcaaaatt ctctaaagca gctcaagaag caattgaagc ggctggtgga tcaatcgagg
    46081 tgatctaatg tttagactat taaaagatgc ttttaaagta aaagacatta gatcgaaaat
    46141 cctatttact gtatttgtgt tgtttgtatt tcgcctaggt gcacatatta ctgtaccagg
    46201 cgtaaatgct gcttcattgc aaaacctttc ttctttgcca tttttcaaca tgttgaactt
    46261 ggtaagtggt agtgcgatgc aaaacttttc cattttctca atgggggttt caccatacat
    46321 tactgcttct atcgtcattc agcttcttca aatggacatc gtgcctcgat ttgttgaatg
    46381 gtcgaaacaa ggggaagttg gtcgtaaaaa actgaatcaa gcgactcgat atctgacatt
    46441 agtattggca tttatccaat ctgtcggtat cactgctgga ttcaatcaat acgctcaatt
    46501 aggtttcgtg aataatccta acgtagcaac ttacgtaacg atcggtttga ttctaacagc
    46561 cggtacgatg tttgttactt ggctaggaga acagatcaca gaaaaaggta tcggaaatgg
    46621 cgtatcaatg atcatttttg caggtatcat ttctagattg cctgaaagta tcagagaaat
    46681 ttatgaagat tactttataa atattgatcc ttcagacata tggaaatctg ctatttttgt
    46741 ggccatttta ctagtagcgg tcttgttgat cgtgacattc gtaacatttt tccaacaagc
    46801 tgaacgcaag atcccgatcc aatatacgaa acgcgtagct ggcgcgccta caagcagcta
    46861 tctgccgttg aaagtaaatg cagctggagt tattccagtt atctttgcta gttcgtttat
    46921 tgctacgcca aatgctgttt tacaagcatt gagcggtaga ttcggttctg aaaactggta
    46981 caaagtgatg atggagatct ttaactataa tacagcacct ggtgctacga tttatacgat
    47041 tctgatcgtt gccttcactt tcttctacgc ctttgttcaa gtaaaccctg agaaattagc
    47101 ggaaaacttg caaaagcaag gaagttatat accaagcgta cgccctggta aggggactga
    47161 agagtatgtc tcaagattac tgatgcgttt aagtacagtt ggtgcagtct tccttggttt
    47221 agtggcattg cttccaatca ttgctcaaat ggtttgggat cttccacaat caatcggatt
    47281 aggcggtaca agcttactga tcgttatcgg tgtagcttta gaaactgcga aacagttaga
    47341 aggcttaatg ctgaaacgta aatatgttgg attcattgag taagaaagcg tgttgaggtt
    47401 tcccctcaac acacgttctc acacatttat taggagggaa aacaatgaac ctcattttaa
    47461 tggggctgcc aggcgcaggt aaaggaacac aagctgaaaa aatcattgat gcttatggta
    47521 ttccgcacat ctcaacagga gatatgttcc gcgcagcgat gcaaaacgaa acagctcttg
    47581 gcctggaagc aaagtcttat atggataaag gtgcattagt tcctgatgaa gtaacaaatg
    47641 gaatcgtgaa agagcgacta gctgaaccag atacagagaa aggcttctta ttagatggct
    47701 ttccacgtac gctagatcaa gcaaaagctc tggacgcaat gctaaaagat ttgaataaaa
    47761 aaattgatgc tgtcatcgat atccatgttg gtgaagaaat cttggtcgaa cgtctagccg
    47821 gccgatttat ctgcagcaat tgtggagcaa cttaccataa aattttcaac cctacaaaag
    47881 ttgaagacac atgtgatcgt tgcggtgggc atgaattcta tcaaagagaa gatgataaac
    47941 ctgagacggt taaaaatcgt ctggctatca acatcaaaaa tagtgaacca atcttggctt
    48001 attataaaga acaaggtttg ctaaacacta ttgatggtgc tcgtgaaatc gatgctgtat
    48061 ttgcagatgt acaaaaaatc attgaaaaat agcacgattg cccgtagccg acttgcatta
    48121 tgcagttact cttgtcaagt tcaataaatt atgatagaat atgtcagtcc gacttctaga
    48181 gaatcctcta gatgatccat tttgagaatt ttgaggtggg aactgcagtt tccgccattt
    48241 tatcgtgtga aaatgcgaaa caagtacaag gaggtactgt gcgtggcaaa agaagatatg
    48301 attgaagtcg aaggtacagt cgtcgaaact ttgccgaatg caatgtttaa agttgaacta
    48361 gaaaacggac accaagttct tgctactgtt tcaggtaaaa ttcgtatgca ttacatccgt
    48421 atcttacctg gtgacaaagt aacggttgaa ttgtctccat acgatcttaa ccgtggccgt
    48481 attacttatc gctttaaata attgtactcc gtaaaactac aggaggtatt atcatgaaag
    48541 taagaccatc agtaaaacca atgtgcgaac attgtaaagt aattcgtcgt aaaggacgcg
    48601 ttatggtgat ttgcccagca aatccaaaac ataaacaacg tcaaggataa ttggaggtgc
    48661 tataaatatg gctcgtattg caggagtaga tattcctcgt gataaacgtg tagtagtttc
    48721 acttacttat atctatggta ttggtaacac tactgctaaa aagatcttag ctgatgctgg
    48781 tgtatctgaa gatgttcgcg ttcgtgaatt aacgaatgaa caaacagatg ctatccgtgc
    48841 agaagtagat aaattaaaag ttgaagggga tcttcgtcgt gaagtgaact tgaacatcaa
    48901 acgtttgatg gaaatcggtt cttaccgtgg aatccgtcat cgccgtggat tgcctgttcg
    48961 tggacaaaac acgaaaaaca atgcacgtac tcgtaaaggc ccagctcgcg ctatcgctgg
    49021 caagaaaaaa taattcttaa gtgaaggagg ttaaaacttc atggcagcaa aaaaagtgaa
    49081 tcgtaaacgc cgtgtgaaga agaatataga agcaggtatt gcacatatcc attctacatt
    49141 caataacact atcatcatga tcactgatac tcacggaaat gcattagcgt ggtcatcagc
    49201 tggttcatta ggtttcaaag gaagcaaaaa atcaactcct tttgccgctc aaatggcagc
    49261 agaaactgct acaaaagcag caatggaaca cggattaaag actgttgaag taacagttaa
    49321 aggacctggt tctggacgtg aagcagcaat tcgttcatta caagctacag gtttagaagt
    49381 gactgcaatt cgtgacgtga ctccagttcc tcataatggt gcccgccctc caaaacgccg
    49441 tcgtgtttaa tgagtgccgc tcattttgag ttttatcagt aacgtcattg aacaagacac
    49501 ttttcgtttt gaaaggggta aagataagaa tgattgagtt cgaaaaacca agaatcgcaa
    49561 aaattgatga agaaaaagat tatggcaagt tcatcgttga acctcttgaa agagggtatg
    49621 ggactacttt agggaattcc ctacgtcgta ttttattatc ttctttgcca ggtgctgcca
    49681 tcactaatat tcaaatcgat ggtgtactgc atgaattctc taccatcaaa ggtgttagag
    49741 aagacgtcac tcaaatcatc ttgaacatta aaggcttagc gctgaaaatg tatggccaag
    49801 aagaaaaaac ccttgaaatc gatattaccg gacctgctac agtaactgct ggcgatatta
    49861 tcgttgatag tgatgtagaa attcttaaca aagatatgta catctgtagt gtctctgaag
    49921 gagctacgtt ccatgctcgc ttaacagtga aacctggacg tggttatgtt caagcagatg
    49981 aaaataaaaa agaagatatg ccaattggcg ttcttccagt cgattcaatc tacacacctg
    50041 ttcgtcgtgt gaactatcaa gttgagaaca cacgtgttgg acaccgtgaa gattttgata
    50101 aattgacgat ggagatttgg acagatggtt caattgaacc gttggaagca atgagtttgt
    50161 ctgcgaaaat catgactgaa catttggata tttttgtgaa cttaactgat gaagcaaaaa
    50221 atgctgaaat catggtcgaa aaagaagaaa cacaaaaaga aaaaatgttg gaaatgacga
    50281 tcgaagagtt agacttgtct gttcgctctt acaattgttt gaaacgtgca ggaatcaata
    50341 ctgtacaaga acttacaaat aaatctgagc ctgaaatgat caaagtccgt aacttaggac
    50401 gtaaatcatt agaagaagtt aaagcaaaat tgcacgactt aggtttaggt ttacgtaaag
    50461 aagattaaaa ccttttacga aggagggaaa ccaacgtgag ttatcgtaaa ctaggacgca
    50521 catctagcca acgtaaagcg atgttgcgtg atttaacaac tgatttatta atcaacgaac
    50581 gtattgtgac tactgaagct cgtgcgaaag aagttcgctc aactgcagaa aaaatgatca
    50641 ctttaggtaa acgcggagat ttgcatgcac gtcgtcaagc tgcggctttc gtacgtaatg
    50701 aagttgccag cgtgcgtgaa gaaaatgagg acatcgtcat tgaatcagct ttacaaaaac
    50761 tgttcaacga tattgcacct cgttacgcag aacgtcaagg cggatacaca cgcattttga
    50821 agacagaacc aagacgcggt gatggtgcac caatggttgt cttagaactt gtctaaaatc
    50881 acactaataa catcacttta tagatgattc ttttagagtg ttatgatgtt ggaagatatt
    50941 tttccgagtc tagctcagcg ctgctcctca agcgaatatc gcttggggag taagcactca
    51001 tcataaagtg tttttttata ccttcaatta cctgtcatga cagatatgtt tgaaggcata
    51061 aaaaaacatt ttaagcgaga aataattgaa aattttgtct ttttttgata gtgttagtag
    51121 gaactttagg atgaagaagg agtaatcatg gagccaatta ttgaattaga aaagataaat
    51181 tataaatacc agccagatga tcttcgcccc gcattgaaag atgtctcctt tacaattgat
    51241 aaaggtgagt ggatcgctat tattggacac aatggttcag gaaaatcgac acttgccaaa
    51301 acgatcaatg gattgcttct gccagaatca gggatcgtga aagtgggaaa tcaaatattg
    51361 gatgaagaaa atatttggac gatccgacag atggttggaa tggtttttca aaatccagac
    51421 aatcagtttg ttggttcgac agtagaagat gacgttgctt ttggtcttga aaatcaaggg
    51481 attcccagag aagaaatgct agtgcgtgtg aaagatgcac ttgagaaagt tagaatggct
    51541 gagtttgctt cacgcgagcc tgctcgttta tcaggagggc aaaaacaacg tgttgccatt
    51601 gccggcgtgg ttgccttacg acctgatatc attattttag atgaagcaac cagtatgcta
    51661 gatccagaag gtcgtgaaga agtgatctct acgatcaaga agatcaaaga agaaagtcaa
    51721 ttgaccgtta tatctatcac tcatgatatt gatgaagcag ctaatgccaa tcggatacta
    51781 gtgatgagac aaggcgaact tgtccgtgaa ggtacaccaa aagaaatttt ttctgctggt
    51841 cctgaactga tcgacttagg tctagatcta ccatttcctg aaaaactgaa gagcgcactg
    51901 aaagaacgtg gggttgatgt gcccagtgaa tatatgacag aagaaaggat ggtggattgg
    51961 ttatggacat ccgttttgaa caagtagact tcacttatca gccgaataca ccatttgaac
    52021 aaagagcctt atttgacatc aatatgacga tcaaagaaaa cagttatact gcattagtag
    52081 ggcataccgg aagcgggaaa tcaaccttac ttcagcattt gaatgcgctt gttaagccaa
    52141 caagcggtac agtccatatt ggagaacgag atattcagcc ggatacggat aataagaatt
    52201 taaagcccat ccgaaaaaaa gttggcatcg tctttcagtt tccagaagca cagctattcg
    52261 aagaaacggt agcaaaagac attgctttcg gtcctaaaaa ctttggtgtc agcgaagaag
    52321 aagcactagt cctagcaaaa gaaacattag aacaagttgg gctggatgaa agctatttgg
    52381 aacgttcgcc atttgaactt tcaggagggc aaatgcgtag agtagcaatc gctggtgtgc
    52441 ttgccatgag gccggaagta cttgtattgg atgaaccaac ggcaggactt gatccccagg
    52501 gtagaaaaga aatgatggag atgttttggc gtcttcataa ggaacagcag atcactattg
    52561 tattagttac gcatttaatg gatgatgtag ccaattttgc agattatgtc tatgtgttgg
    52621 aaaagggacg aatcgttaat agcggggagc cgcaagaggt ctttcaaaac attgaatggc
    52681 taaaagaaaa acaactaggc gtacctactg ccaccgagtt tgcagaagaa ttaatggcta
    52741 aagggatgaa ctttgctacc ttgcctttga cagctgaaga attagcagac gctatcgtcc
    52801 attatgctgg aggaaatgcc catgatgaat aaattgatat ttggtcgtta tatgcctgga
    52861 gattccttga tccatcgatt ggatccccga gctaaattat tagccagttt ttattttatc
    52921 ggaatcattt ttttagcaaa taattgggaa acgtaccttt tgttggcatt tttcacatta
    52981 ctttctgtct tcttgtcaaa agtagatttg aaatttttta tccgaggcat tcgcccattg
    53041 atctggctaa tcctcttcac tgtagctttg caggtcttgt ttacaagtgg cggagaaact
    53101 tattggagtt ggggtgtttt ccatatcact gaatttggtg tgatgaacgg cctctttatt
    53161 ttctgtcgat tcgtgttgat catttttatg tcgactctac tcacgcttac tacaccgcct
    53221 ttggaattat cggatgcgat tgagtacttg cttcgtccat tgaaagccgt acggttccca
    53281 gtccacgaaa tttcattgat gctgtcgatt gctcttcgtt ttgtgcctac attgatggac
    53341 gaaacagaaa agatcatgaa cgctcagcgc gctcgcggtg ttgattttgg tgaaggaaac
    53401 ctagttcaga aaatgaaagc agtcattcct ttgctgattc ctttatttgt cagcagcttc
    53461 aaccgtgcgg aagatcttgc gactgctatg gaagcaagag gctacaaagg tggggaagga
    53521 agaacgaaat atcgtgtatt gcattggcat aatagagaca cgatggttat cattgctttt
    53581 gtattattaa ctgtgctact gatctttttg agaggataaa cgttcagtgg gccgctatct
    53641 attcttttct tgctgatctg tttgcaagaa aggatgcgag cagtgtatga caagaaatat
    53701 tagggtctgt ggcagactaa ggtcacaggc ctctttcttt tatctgaaaa gaaagaacga
    53761 aaaaatttta taaaaaaatc attattccag ataacctttt aggaacaaag gagagaaaaa
    53821 catggtacgt tacaaagcaa tcattgcata cgacggaaca aattttaatg gattccaaaa
    53881 acagccaaat gggcgtactg ttcaagaaga agtagaaaaa acattacaaa aaatggcaaa
    53941 tggaaaagaa atcacggtct ttggttcggg aaggacagat gccggagtac atgcgattgg
    54001 gcaagtaatc cattttgatt atccagagga gcgtccgtta gaacgaatgc gtttcgcgct
    54061 agatacccag tcaccagagg atattgctgt aagacaagta gaaattgttt cagaagactt
    54121 ccacgcaaga tatttggtca aagaaaagac gtatcaattt cgagtggata tagggaaacc
    54181 acgcagtcct tttcgacgcc attatgcaag ttattttcct taccctcttg atttggaaaa
    54241 aattcagcgg gcgcttcctg atttattagg gactcacgat ttcacctctt tttgtgcatc
    54301 aggaagctcg atagaggata aagtacgtac gatctacgag gctaagatgg aggtaaatga
    54361 aacaaaagac gaattgttgt ttacatttag gggaaatggc ttcttgtata agatgatacg
    54421 gatcttagta ggaacgcttt tgaaaattgg caatggccga ttgcctgaag atagtatccc
    54481 agatattatt gcgaaaaaag atcgtaatgc agcaggacct acagcacatc ctgaaggact
    54541 ttatttgtat gaagtgaaat atgaataaga ggttgtgaca gaagtggtca gcttcaagaa
    54601 ataagaagga atttacgaaa attacttctc aaaattttgc aaaatttcgg cttattttcg
    54661 aaggagctac ttctggaaca ccgtgaatca gaggttgtga aaaagctgcc ctgcatcaag
    54721 taataagaac aaccaaacaa aaatagctcg tcatattttt cgtttggttg ggcttattac
    54781 cgcagatgca agcttttgaa caccgtttaa ggaagaggtt gtgaaatcag cgttttgacc
    54841 tgagtattag agaaacaaac ggaaaaatag ttcctcatat tttgccgttt gttgagctaa
    54901 taccgaaggt cagctgattg aacaccgttt aagaaagagg tcgtgagcct gtcatttaac
    54961 tctgagtcac aagtaacaat ttcaaaaaac agcttctcat ctttgatgaa tatcgtgact
    55021 tgtggacgag gcgttgataa gtgaatcatc tttttgttac ctattttttc tgtaaccaaa
    55081 aaacgatgga acgttgtttt gattgccctt aatgaggaca gtcggaacag gtttgtcatt
    55141 tttatattta aatggcacaa atttttctaa ttatttgtca tttagcgctg aaaatttttg
    55201 ctatttatct aaaaaagtga taactactta agatgaagca gaatatttac gccatacggc
    55261 atacaaccgc gaaaaatata aaaaaaagaa aacgattgaa cgcgtttttg cagtcgcaaa
    55321 ggaaaagcat ggaatgcgct acatcaagta tcgagacttg gaaaacaatc aggtgtatac
    55381 gctgattgtt tttgctgcaa tgaatctgaa aaagttgtcc acttggcagt tggaacatat
    55441 gtcttcatat cttttattat acaaaaatag caaaagcacc agtctacact cttagtaaag
    55501 aggaaactga tccttttgtc tacattctta aagtacttaa actaagtact ttattttact
    55561 ctttaggatt gatacagtag catctatccc tttattaata atcttttatt tttttaaata
    55621 aagatgttgt ctccatcttc aggcactaaa actttttcgg aaattccttt ttctttattg
    55681 aaagacttta attcttctct tgacagtccc caatggttta cagcttccat atgaacggca
    55741 ataattgtgg aatctggtat tgctttatgc aattcatata aatcgtcttt ccccataata
    55801 agagattctc caactaaaaa atgattatct ccagcatttg ctactatgat ttctggatta
    55861 tactgatcaa tagtttgttt aacagcttca taccaaactg tatctcctgt aaagtatagt
    55921 gtcttctcag tcgaatgttt gaaaacaata ccgcaaacat tacctacata attcaagatt
    55981 tctcctctgc catgtttgcc ttttgtttta accaattgta tagattcaaa aactgtgtct
    56041 tctcgtagta cctccacatt tttaaaacca tatagtcgaa tttcattcgc atcattttca
    56101 tcttgcacaa ataattttat ttctttaggt aggacctctt tcgctatatc gtcccaatga
    56161 tctagatgta aatgtgttac aataacagca tcaataccgt tgataatatc ttcgactggc
    56221 attggcaaat caactagagg attcatttga tcttttcttg gtgatggaaa aggacctttc
    56281 gaaataaatg gtggaagtgt tcctttttcc gctaacattg gatcaattaa aaatttttta
    56341 cctccatagt ctaagatagc tgttgcatta cgaatgtgat gtatattcat aaacgttgtc
    56401 tcctttttgt tgatatgaaa tatttttagt ataattcaat tccatacaat aaataaaccg
    56461 aacaaaaaaa gtcttttact at
//
LOCUS       DANKKG010000003        56102 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000003 DANKKG010000000
VERSION     DANKKG010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.003"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..418
                     /locus_tag="PW004_000124"
     CDS             <1..418
                     /locus_tag="PW004_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDL2725125.1"
                     /translation="KNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKV
                     KNEKISLFNILKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYP
                     EEAVREITDNINKLEQTISSIGKKYTKKKMKGGDTI"
     gene            457..1599
                     /locus_tag="PW004_000125"
     CDS             457..1599
                     /locus_tag="PW004_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2725126.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(1694..3085)
                     /gene="sufB"
                     /locus_tag="PW004_000126"
     CDS             complement(1694..3085)
                     /gene="sufB"
                     /locus_tag="PW004_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HDL2725127.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(3090..3560)
                     /locus_tag="PW004_000127"
     CDS             complement(3090..3560)
                     /locus_tag="PW004_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HDL2725128.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(3547..4782)
                     /locus_tag="PW004_000128"
     CDS             complement(3547..4782)
                     /locus_tag="PW004_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDL2725129.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(4779..6065)
                     /gene="sufD"
                     /locus_tag="PW004_000129"
     CDS             complement(4779..6065)
                     /gene="sufD"
                     /locus_tag="PW004_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HDL2725130.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(6078..6851)
                     /gene="sufC"
                     /locus_tag="PW004_000130"
     CDS             complement(6078..6851)
                     /gene="sufC"
                     /locus_tag="PW004_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HDL2725131.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(7048..7896)
                     /locus_tag="PW004_000131"
     CDS             complement(7048..7896)
                     /locus_tag="PW004_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDL2725132.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(7920..8606)
                     /locus_tag="PW004_000132"
     CDS             complement(7920..8606)
                     /locus_tag="PW004_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2725133.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(8599..9633)
                     /locus_tag="PW004_000133"
     CDS             complement(8599..9633)
                     /locus_tag="PW004_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2725134.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     misc_binding    complement(9696..9952)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(10028..10366)
                     /locus_tag="PW004_000134"
     CDS             complement(10028..10366)
                     /locus_tag="PW004_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HDL2725135.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(10368..10724)
                     /locus_tag="PW004_000135"
     CDS             complement(10368..10724)
                     /locus_tag="PW004_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HDL2725136.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(10730..11917)
                     /locus_tag="PW004_000136"
     CDS             complement(10730..11917)
                     /locus_tag="PW004_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HDL2725137.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(12108..12953)
                     /locus_tag="PW004_000137"
     CDS             complement(12108..12953)
                     /locus_tag="PW004_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL2725138.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            13187..14152
                     /locus_tag="PW004_000138"
     CDS             13187..14152
                     /locus_tag="PW004_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HDL2725139.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            14529..15572
                     /locus_tag="PW004_000139"
     CDS             14529..15572
                     /locus_tag="PW004_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HDL2725140.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(15634..16098)
                     /locus_tag="PW004_000140"
     CDS             complement(15634..16098)
                     /locus_tag="PW004_000140"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDL2725141.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            16344..17291
                     /locus_tag="PW004_000141"
     CDS             16344..17291
                     /locus_tag="PW004_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL2725142.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(17502..19574)
                     /locus_tag="PW004_000142"
     CDS             complement(17502..19574)
                     /locus_tag="PW004_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDL2725143.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(19507..19884)
                     /locus_tag="PW004_000143"
     CDS             complement(19507..19884)
                     /locus_tag="PW004_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HDL2725144.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(20013..20591)
                     /locus_tag="PW004_000144"
     CDS             complement(20013..20591)
                     /locus_tag="PW004_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2725145.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            20723..22015
                     /locus_tag="PW004_000145"
     CDS             20723..22015
                     /locus_tag="PW004_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDL2725146.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(22075..23193)
                     /locus_tag="PW004_000146"
     CDS             complement(22075..23193)
                     /locus_tag="PW004_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HDL2725147.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(23207..24628)
                     /gene="arcD"
                     /locus_tag="PW004_000147"
     CDS             complement(23207..24628)
                     /gene="arcD"
                     /locus_tag="PW004_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HDL2725148.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(24874..25995)
                     /gene="mnmA"
                     /locus_tag="PW004_000148"
     CDS             complement(24874..25995)
                     /gene="mnmA"
                     /locus_tag="PW004_000148"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HDL2725149.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            26399..26998
                     /locus_tag="PW004_000149"
     CDS             26399..26998
                     /locus_tag="PW004_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HDL2725150.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(27136..28422)
                     /gene="efmA"
                     /locus_tag="PW004_000150"
     CDS             complement(27136..28422)
                     /gene="efmA"
                     /locus_tag="PW004_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HDL2725151.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(28693..29037)
                     /locus_tag="PW004_000151"
     CDS             complement(28693..29037)
                     /locus_tag="PW004_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HDL2725152.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(29174..30319)
                     /locus_tag="PW004_000152"
     CDS             complement(29174..30319)
                     /locus_tag="PW004_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDL2725153.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            complement(30499..31473)
                     /locus_tag="PW004_000153"
     CDS             complement(30499..31473)
                     /locus_tag="PW004_000153"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HDL2725154.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(31553..32428)
                     /locus_tag="PW004_000154"
     CDS             complement(31553..32428)
                     /locus_tag="PW004_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HDL2725155.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            32558..34465
                     /locus_tag="PW004_000155"
     CDS             32558..34465
                     /locus_tag="PW004_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HDL2725156.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGATPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(35006..38284)
                     /locus_tag="PW004_000156"
     CDS             complement(35006..38284)
                     /locus_tag="PW004_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HDL2725157.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(38555..39100)
                     /locus_tag="PW004_000157"
     CDS             complement(38555..39100)
                     /locus_tag="PW004_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL2725158.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(39112..39804)
                     /locus_tag="PW004_000158"
     CDS             complement(39112..39804)
                     /locus_tag="PW004_000158"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HDL2725159.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(39823..40107)
                     /locus_tag="PW004_000159"
     CDS             complement(39823..40107)
                     /locus_tag="PW004_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725160.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(40120..40683)
                     /locus_tag="PW004_000160"
     CDS             complement(40120..40683)
                     /locus_tag="PW004_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDL2725161.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            40911..41573
                     /locus_tag="PW004_000161"
     CDS             40911..41573
                     /locus_tag="PW004_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HDL2725162.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            41697..42893
                     /locus_tag="PW004_000162"
     CDS             41697..42893
                     /locus_tag="PW004_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HDL2725163.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(42994..44370)
                     /locus_tag="PW004_000163"
     CDS             complement(42994..44370)
                     /locus_tag="PW004_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2725164.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(44407..45273)
                     /locus_tag="PW004_000164"
     CDS             complement(44407..45273)
                     /locus_tag="PW004_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HDL2725165.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(45362..46387)
                     /locus_tag="PW004_000165"
     CDS             complement(45362..46387)
                     /locus_tag="PW004_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL2725166.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(46405..47079)
                     /locus_tag="PW004_000166"
     CDS             complement(46405..47079)
                     /locus_tag="PW004_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2725167.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(47263..48156)
                     /locus_tag="PW004_000167"
     CDS             complement(47263..48156)
                     /locus_tag="PW004_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL2725168.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(48230..49927)
                     /locus_tag="PW004_000168"
     CDS             complement(48230..49927)
                     /locus_tag="PW004_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HDL2725169.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(49920..51233)
                     /locus_tag="PW004_000169"
     CDS             complement(49920..51233)
                     /locus_tag="PW004_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HDL2725170.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(51233..55270)
                     /locus_tag="PW004_000170"
     CDS             complement(51233..55270)
                     /locus_tag="PW004_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDL2725171.1"
                     /translation="MVVDERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSG
                     QALQRIQRQLFNGILERLGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNW
                     TQSNERYQGDKGSPYDGENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTV
                     QLWGNERNFENSYLDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKK
                     EISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVN
                     FYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETLY
                     KDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVL
                     FRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSS
                     NNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIG
                     RNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSP
                     FGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKF
                     SPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSSS
                     IRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETK
                     MPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEYT
                     SGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNIN
                     KLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGK
                     YVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLY
                     DNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLD
                     GAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPL
                     HLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDG
                     VLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDS
                     SLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLK
                     VEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILL
                     AAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(55283..55741)
                     /locus_tag="PW004_000171"
     CDS             complement(55283..55741)
                     /locus_tag="PW004_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL2725172.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
BASE COUNT        15752 a        12087 c        10122 g        18141 t
ORIGIN      
        1 taaaaacctg ttccagcatc ttcgtcaaca gttcccacca gtacactcta atttagatac
       61 atggctggat agatattcca aattagaacc agagatgcaa gaatttattg ctaacaagac
      121 ttgtaataaa gttaaaaatg aaaaaattag tcttttcaat atcttaaaaa tagaagaatt
      181 attcatcaat gctattgtag aagaatttga caataacatt cttttcctca ctagcagtat
      241 agaagaacta ctagaaagaa tggtagatga atggctacct attcaattga aagacatgtc
      301 ttatcccgaa gaagctgtga gagaaattac tgataatata aacaagctag aacaaacgat
      361 ttcatcaatt gggaaaaaat acactaagaa aaaaatgaag ggaggtgata ccatctagtg
      421 gtaagtactc cttgtaaact aagaggagat gatttcatgg caaaaatatc aaagtacaaa
      481 aagaaaaacg gtcaagtggc gtggatgtat aaaggacaca ttgccaccga tccacgaact
      541 ggtgaaaaaa taaatactac gagaagaggt tttagtagca aacaagaagc tcaaaaagat
      601 tatgacgagt acagacatca aatattatac ggtgttaaaa agaaagcacc tgatatgaca
      661 tttgaagacc tgtataatga atggataact catcagcgta ccagtgttaa agcttcaaca
      721 atcgcaatct cagtaagata cgcaaataat caaatacttc cagcatttgg taagcttaaa
      781 ctatcgaata tctctgtccc atattgtcaa aaagtggtag atgaatggca cagtaaatat
      841 gaaagttacg attatatgag aaaacagact gcacaaattt tacgctacgg ggttgcaatg
      901 caatatattg acaataatcc tatggaaaaa acacttcttc cacgaaaaaa agagtatgag
      961 aaaaatcgta aattttactc aaaagaggag cttaataatt tactggatgc ttttaaagat
     1021 tttggtaaca tgaaacaata cgccttcttc agattattgg catacacagg tatgagaaag
     1081 agtgaagtac tcgctttaca gtggaaagac atagacacgt ttaataaaga actacatgtc
     1141 aataaaactc ttgctgtaga tgaatttggt aaagttataa ttcaatctcc gaaaacaaga
     1201 gcttctagac gagttatctc tttagatact gagactcttt ctatcctcaa caactggaaa
     1261 ttacagcaaa aagaagagta tttaaaactt ggatacaata ctagctctaa agagcaacat
     1321 gtttttacaa cagttaaaaa cactctatac attccaaaca ctgttaatga ttggttaaga
     1381 tatattttga aaaaatataa cttgcctcgt attacacctc atgggtttag acatacacat
     1441 gctagtttat tattagaagc aggagaatct gtcaaggtag ttcaacaacg attagggcat
     1501 gaaaatagta aagtgacatt agacatctat gcacatatta caaataatgc acctaagaaa
     1561 acaggtcaag actttgctga tatgatggct catcagtaaa agagctggtc aaaatctggt
     1621 catttctagt atacaggcaa aacaaaaaag ctagaaccct tatatatcaa gggttctagc
     1681 tcctataaca ggtttaccca acactgcctt ccatctcata actgatcaat cgattgagtt
     1741 cgactgcata ttccattgga agttctttgg tgaatggttc aacaaagccc atgacgatca
     1801 tttcagttgc ttcagattcg gtcaggcctc ggctcatcag atagtagagt tgttcttctg
     1861 agatttttga gactttggct tcgtgttcta aagagacttg gctgttgtgg atctcattga
     1921 atgggatcgt atcggatttc gaccattcgt ccatgatgat cgtgtcacat tcgatatgag
     1981 agatcgaacc cgcactgttt ttggcaaagg ttacttgtcc tcgatagttt acttctccgc
     2041 cgtcttttga aattgattta gagacgatag agctagatgt atttggtgca ttgtggatca
     2101 tttttgcacc cgtatcttgg atttggtttg ctcctgcaaa ggcgatggag agcatggtgc
     2161 ctcgtgcacc ttttccatct aagtaaacac ttgggtattt catcgttgtt tttgcaccta
     2221 ggtttccgtc gatccattca acggttgcgc cttcgtatgc ttttgctcgt ttggttacta
     2281 agttgtacac attgtctgac cagttttgga tcgttgtgta acggcaatac gcatcttttc
     2341 ttgtaaagat ttctacgatg gcggcatgca ggctgttact tgaataagta ggtgccgtac
     2401 aaccttcgac atagtggatg cttgctcctt cgtcaacaat gatcagcgta cgttcgaatt
     2461 gccccgtgtt ttccgcattg atccggaagt acgtttgtaa tggaacatcg actttgacgc
     2521 cttttggcac gtaaataaag gtcccacctg accatacggc tgaattcagc gctgctaatt
     2581 tgttgtcggt tggcggtaca agttttgaaa agtactcttt gaataattca gggtattctt
     2641 tcaaagcaga gtcagtgtca gtaaagacga ttcctaactt ttcgaattca tctttcatat
     2701 tatggtacac gacttctgat tcatattgtg cggaagcacc ggctagataa gctcgttcag
     2761 cttctggtat accaatcttt tcaaatgtat ctttgatttt ttctggtacg tcttcccagt
     2821 cacgagcggg tttgtcactt ggtttttggt aatatttgat ggcatcgaaa tcgatgtctg
     2881 aaaggtcggg gccccatgtt tgcattggca ttttgttaaa tgtttctaga gatttcagac
     2941 ggaattcaag catccattct ggttcgcctt tgattcttga catctcacgg atcacttctt
     3001 ctgtcaagcc ttctcctgta ctgaaaaccg gttcgacatc atcgtgaaac ccgaatttat
     3061 attcttctaa ttctggtacg cccatgttct cactcctctt ccttgtggta atgatggaaa
     3121 gctgcggtac catcgttttc cattgctttt cctagtgctt tccaggcaag tgtcgcacat
     3181 ttgatccgag cgggaaattt agctacacca ccaagcatag cagcatcgcc taattggtcg
     3241 ggatctttga cttcttttcc ttggacaagc tgtagaaagt ctacagttaa atgttctgct
     3301 tcttctattg attttccgat gacggcgtct gtcatcatcg aggcacttgc cgtactgatg
     3361 gagcagccac tgccgtcaaa ggcgatattt ttgatgattc cgtcttttac atcgacttgc
     3421 aattcgatca catctccaca tgtggggttg ttcaattcga tcttgcttgt cgagtcagtc
     3481 aagcttccac gatgatgcgg atgggaagaa tgatccaaga tcacttgacg gtataaattg
     3541 tctaatttag atagtcccat gttggaaaaa ctcctttgtt gccatgattg cttcaactaa
     3601 acggtcggcg tcttgcgttg tgttgtagaa ataaaagctc gcgcgagcag tcgctgatac
     3661 ttcaaggtac ttcaatagcg gttgcgcaca atgatgacct gctctgacgg ctaccccttc
     3721 catgtctaaa gctgtcgcga catcatgggg atgtaaccca tccaaattga atgcaacgac
     3781 tcctgtccga aaattcgggt cttgtggacc ataaacagtc aatccatcaa ttgcttgtaa
     3841 ttttggcaac acataagcgg ctagttcttc ttcatatcga tggatattgt ccataccgag
     3901 tttttgcaaa taatcgattg catggcctaa tgcgatagcg ccggcgatat ttggtgtccc
     3961 tgcttcgaat ttccaaggta gttctttcca tgtactgtcg tataattcga caaaatcgat
     4021 catttcgcca ccaaattcaa caggttccat ttcttctaac cattttcgtt taccatataa
     4081 aacaccaata cctgttggtc cgcacatttt gtgtccgctg aaagcaaaga aatcacagtc
     4141 tagtgcttgg acatcaatgg ccatgtgcgg ggcagattga gcaccatcta ctacgatgac
     4201 cccgccgttt tcatggacaa gttcagctaa ttcttgaaca ggattgaaga cacctaagac
     4261 attggataca tgagcaatgg agacgatttt ggttttgtcc gtaatctttt tcgctgcatc
     4321 ctccatatcc aggaagcctt cttctgttag ttcgatatat ttcaagacag cacctgttcg
     4381 ttttgccaac tgctgccacg ggataacatt agaatgatgt tccatgtaag agagcactat
     4441 ctcgtctcct gctttgatat agcggtcgcc gaaggttctg gctacccaat tcaagcttgt
     4501 cgtcgtacca cgagtaaaca gcacttctgc tgtttctttt gcatggatga acgcccgtac
     4561 tttttctctt gcttgttcat aatcatgggt cgcgcgttct gctaacgtat ggacaccgcg
     4621 atgaacattg gcgttatcgt gttcgtaata gtacgctaat gcctggagta cttgttttgg
     4681 cttttgggtc gtcgctgcat tatccagata aactaaaggt tcatcattga cgtcttgaaa
     4741 taagatagga aaatcattgc gtagcttttt agcatcgatc atccattcaa cttcccttcg
     4801 attacttcga ctaattcttt ccgaacttgt tctacaggga ttgctgttaa gacagaaccg
     4861 aggaatcctc gaatcactaa gcgctctgct tcttctttat gcaatcctct actcatcaga
     4921 tagtacattt cttctggatc aactcgtcct acactggcag catgtccagc agttacttcg
     4981 ttttcatcga tcaaaagaat aggattcgca tcaccacgtg ctttatcaga aagcatcaag
     5041 acacgacttt cttgttgggc atctgcacct tttgcccctt tcaagatgtg gccgatacca
     5101 ttaaaggtca atgttccttt ttctcggatc actccgtgtt gcaaaatatg cccgatcgta
     5161 tgaggggctt tattggttac tcgtgtatcg attccttgga tttgtctgcc cgagctgatt
     5221 gccacgattt tgacttctgc gtgtgcacct tctcctgcta aatctgagtc aaagtcagcg
     5281 atcacatgac cgtcattcat gacaccaatt gcccagtcga cagaagcatc tcgtaaaatg
     5341 tgcccacggc gattcatata agaagtaata ttttctccta attgatctac ggctgaatat
     5401 ttgatttttg aaccagcttt agcgatgact tccacgatga tattacctga tgattttgct
     5461 acttgttctt tggttgtctg gaaacgttcc agataagaaa attcactatg ttcatctgcc
     5521 acgatcaaca catgcttgaa gaagtgctca tcggatgcgc catcttgaat gaataaggac
     5581 tcgagtggtt catcgatcac gacattttta ggcacataca ggaaaatacc gctgttcata
     5641 aaagctacat ggagtgcagt caattggtct tcgttcatgt ttactgcttt tttcatataa
     5701 tattcttgga ctagttctgg atattcgatc attgcagtga acaagtcggt aaaaatgact
     5761 cctttttctg ctaattccac aggcagttgt tcaaaaatcg tccatgaacc ttgttggaca
     5821 agcacaggat tgtctttcat tgcatcaaat gccggaatcg tgcctgtttc actgatggtt
     5881 tgtgtttctt tcacatcaaa taacgaccag cgatggaatt tcacccgatc gatatgcggc
     5941 aatggcagtt catctgcttt cgctagtgcg tcttgacgga aggttctcat ccaatcaggt
     6001 tcgcctttac ttaaagaaaa atcatttacc gcgtcaagat aatcgagcca gtttttttct
     6061 ttcattcttg ctccccctta cgcttcttct tctttatagt cgattcctaa ttctttactg
     6121 ataccagcgt agccttctgc ttccagacgt ttagctaggt cagcattccc tgtcatgact
     6181 actcggcctt ccatcatgat gtgaacgaca tcaggtgtga tatagttcaa taatcgttga
     6241 tagtgggtga tgatcaatgc accgaaattg tctccgcgca tttcatttac tcctttagca
     6301 acgactttca aagcatcgat atccagacct gaatcgattt cgtctaaaat tgcaaatgta
     6361 ggttctaaca tcaacaattg caggatctca ttccgtttct tttctccgcc agaaaaacct
     6421 tcgttcagat aacgttcagc catttcttct ggcatattta gtaaagccat tttttcatct
     6481 aactttttca aaaatgccat aacagaaatt ttgtcgtttt catcacgttt cgcattgatc
     6541 gctgcacgca tgaattccgc attcgtgata cctggtatct cacttggata ttgcatagct
     6601 aaaaataagc caagtcgcgc acgttcatct acttccaagt ctaaaacatt tttcccgtca
     6661 aacaaaattt cgccttctgt tacttcatag tttggattgc ccatgatagc ggcagataag
     6721 gtagattttc ctgtgccgtt aggtcccatg atggcatgga tttctccggt tttcattgtt
     6781 aggtttacgc ctttaaggat ttctttttcc tcgatcgaca catgtagatt ttttatttct
     6841 aagacagaca tgcattatac ccccaaattc tctttcgttc tcactcattt tagactaatt
     6901 gagaatgaag cgcaatgacc ttccttacta aaatcaaata tcttttattt attattaaac
     6961 aattttgatt tctaaattag aataggtata aaaaagatga gaaataagcc attacagact
     7021 tatttctcat cttttttgtt ccatacctta ttttgcatca gcctttactg gtttaacaga
     7081 gccgccccat ttgtctgtga tccattcttg gacttctttg cggtgaagca cttcaatcag
     7141 ttttttgatt ttgtcactgt cttcatcacc tttacgagtc gcaataatat tcacgtaagg
     7201 agagttgtcc ttttcaagca atacggcatc ttctaatgga ttcaaccctg cgccgtaagc
     7261 gaagtttgcg ttgattgcaa ctaggtcacc ttcatcattt tggtacgtag aagctaaaag
     7321 tgatgggtcg atcgtatggt tgaacttcaa gttcttaggg ttttcttcaa tatcatcgaa
     7381 tgttgctgtt tccagatcta ctccatcttt gactttgact aagccagcat cttgtagaat
     7441 tgtgatgatg cgtccccagt ctgattcaga atttgaagta atgattgttg caccatcttt
     7501 taattcggaa acatctttga cttttttaga gtacaagccc attggttcga tgtggacagc
     7561 accggcattg acaaagtcat atcctttttc tttgatttct ttgttcaaat aaggaatatg
     7621 ctggaagtaa ttggcatcaa tgtcgccttc ttccaaagat tggttcggta atacatagtc
     7681 atcgaatttg acgatttcaa gatcgattcc ttcatctttc aataatggtt tcacatgttc
     7741 caaaatctct gcgtgcggtg tcggtgaagc gccgactttc aatgtcgtgt cgtctgcttt
     7801 atttgctgaa tctttgctgt cgctgttgtt accgcatgct gctaatccta cagtcagcaa
     7861 aagcgctgct gcaaatccaa aaattttctt tttcatatcc tacatccccc tatatcctat
     7921 tatcttttgt ctgttttctt agttaagaaa tcaccgattc cttgaacgat aaatacgatt
     7981 agtaaaataa ttaaagtcgc aacgaatgtc actgttagat taccacgctg gtagccttcc
     8041 tgccaagcaa gacctccaag tccacctgcg ccgatcgcgc cggccatcgc agtaaatccg
     8101 atcatcgaga tcgttgtaac tgttaatcca gaaatcaatg cgggaatact ttcaggtatc
     8161 aagaccttcc agatgatttc ccagtaactt gctcccattg catctgctgc ttccagtaca
     8221 ccagggctga cttcacgaaa agcaatctct actagacggg cataaaatgg tgctgcggat
     8281 aaaacaagag ctggaatggc tgcttgcgca cctagcatag tgcctacgat tgctttagta
     8341 aatggaatga tcaaaaccat caggatcata aatggcgttg accggaaaat attactgatg
     8401 atcgatacga ttccataaat gattcgagca tacggtttgt ttttgcgtcc aatagagtaa
     8461 aggatcaatc ccaaaactaa tccgatgatc gtcactaaga ccattgagat taatgtcata
     8521 tatagtgtat caaggatagc ctgattcatc gtttccgggt tgatttgttt aaaatctaga
     8581 tatgtttctg aaaagctttt atccacgatg gatcacctca attcctactc cttgttcttt
     8641 gatgaacact tctgcttgat ctaattcttt ggtttcacca gtcatcaaga cagtcaatga
     8701 accaaaggaa ccttctttca cttgtttgat ctttccatac acaacgttga catcgatgtt
     8761 atagcgtcga atcgcttggg aaatgactgg ctcgttggca tttctttcgc taaaggtcaa
     8821 agttgccaaa cggccgcttg gattttcttt gatgagttca gctaatagtt ctgtcgaatc
     8881 agcttctggt tcgatgtctt gttgtacaaa gcgtttggtt acttcttttt tcggttgtct
     8941 gaagacagtc aatacgtctc cttcttctac gactttcccc aactccatca cagcgacttt
     9001 gttgcagatt ttgcggatca cattcatttc atgtgtgatc aagacgatgg tcaaattcaa
     9061 tttcttattg atcgctaaca ataagtctaa tacttcttct gttgtttgcg ggtccaatgc
     9121 gctagtagcc tcatcacaaa gcaggatctc aggatcattt gccaatgctc gtgcgatacc
     9181 gacgcgttgt ttctgtccgc cggaaagttg tgaaggatat gcttgtccgc gtccttctaa
     9241 cccgactaaa cgcaacagtt cttctgcccg ttctttacgt ttgctctttg gtacgccagc
     9301 taattccagc ggcagctgga tattttcaag tacagttctt gaccataaca ggttgaaatg
     9361 ctggaagatc ataccgattt tctttcggaa tgtacgtaat tctttgtttt ttaattttgt
     9421 aatatcttga tcgtttacga tcacttcgcc gctcgtggga agctcaagtc cattcaggag
     9481 acggactaat gtactttttc ctgcgccaga atagccgacg attccataaa tatccccttt
     9541 ttcaacgttt agtgaaacgt cattgacggc ttggatgttt ccgtttttcc cattgaatgt
     9601 ctttttgaca ttcgttagtt ggattaatga catgccattc acatctttct cttataattt
     9661 ttcaaacaaa aaagccttcg ccctcttgcc ttaagcaaaa ggacgaaagc ttgaacttcc
     9721 gtgttaccac cttgatttac cgttacttcg caataacagc ctcttccagt acttggactt
     9781 agcttctttc cgtatactgt cgcgctgtaa cgggcactcc cgtagtaagt ttgcttacgc
     9841 tcgcttactc tgctctaaga ccatcttcca tctcattttc gttacccatt ttcagcaaac
     9901 agggctctct tcaaacgtcc gtgacatgta ctcttctttt caaagctttc tcgtttcata
     9961 taggattgct tatgattata gcaactggaa gaatcagtgt caactttttt caagtgattt
    10021 tctgatttta taattcgtca aattcttttg gatcaacatc tttgaaactt aagatccacg
    10081 cattcatttc atctgaatca ttcaagatat tgatatcttc accgatttta tcattaacag
    10141 aagaaactgt acccgttagc gggctggcaa attcattgac cgctttttcc gcttcaactt
    10201 cgatcagagg ttcatctttt tttagcgatt gtcctgcttt tggcagattg gcaaacgtga
    10261 tgtctccaag ttcttcttgc gcttcatttg tcaaaccaac acagtattct tttccattaa
    10321 ataagatcca aaggttgtcc tttttcttca aatgcttgcc tgccatttta tatcagttct
    10381 ccttcataaa ttttttcatt gaatccaatt gcaacaagct ggtcctctcg taagatcagt
    10441 ggtcgcttga tcagcatacc atctgtcgat aagacttggc tcgcttttga agccgtaaac
    10501 tcgcctactt gatctttcag tccaagttcc cgatatttca tcccgcttgt gttgaacagg
    10561 cgacggattg gtacttgact cgtttctagc caagtctcaa tcgttgaggc cttcggtact
    10621 tctttgatca tatcaacggt ttgataacta acgccatgct ggtctaacca cgctttggct
    10681 tttttgcatg ttgaacattt cgggtaccag taaaaggtgt acaattttct tacctccttg
    10741 aagatcttgc aggtgcttca ttttggtatc tcatcgataa tatcaacccg ataccgatca
    10801 tgttccctaa aatcgaggat cctccctgac taatgaaagg caatgggatc cctgttaaag
    10861 gaagtaatcc gatatttgct ccaatattct caaagacatg gaacagcagc atcatgatca
    10921 aaccgcttgc gatataggcg taaaattcat tattcgtatc gaaacacacc cgaatcatac
    10981 gatagattaa aataaaatac aagaaaatga cgaatgcacc accgataaag ccgaagtttt
    11041 caccgataac ggtaaaaatc atatcggact cacgaacagg cacatagata ttgcttacgt
    11101 tgaatccttt accaaacaag ccaccggtac caattgccaa aagtccttgg gccggctgat
    11161 aactcattcc tgacgtatca tggaaagggt ccagccatga atcaatacgt gcaaactgat
    11221 aagatttgaa tccaacttta taaagcaagt ctcttccgcc ttcagtcgta accaagaaga
    11281 tcgttcctgc tccaacgact actgccaaga ttactacagg tgcgatgatg cgccaagaga
    11341 ttccagacat caggaaaatc ccgccaaaga tcgcaagaaa gacaagcatc gttccaaagt
    11401 catcttgggc catgatcaat gctaaaaccg gtattgtcac gatcaagagc ttcccaatca
    11461 gccaaaagtc cgatttcatc gtccgttctt ttacttgcgt attatgttta gttacgatca
    11521 gtgccatcat caagatgtac gcaatcttca tcaactctgc tggctggaaa gtaaacgcac
    11581 cgaatcgaaa ccagttttta gaccctgttg aagcagctaa attctgatca tagaatttga
    11641 gcaagagtcc cattaccact aaccccaaac cataaaggta aggagtcagt ttccagatcc
    11701 atttggaatt caaatgcatg atgacaacga ttgctgccgc tccaactaaa taccagacaa
    11761 cttgcatccc tacgcctttc aagactgctg gattgttcgg atcatggcta agtgctacat
    11821 ataatgagag cagaccaatc aaacataaca gaaagactgg taaaatcacg ccataatcaa
    11881 tacgattatc gttcgaagct tttaattttt tttccatgtt tcatcccttt tcttaaaaac
    11941 catactgctg attattatag cggtccagtt agataatgga aagtctttcc atagacattt
    12001 gggaaaaatt aactttccgt ttgtgcagtt gcttgagcaa atgcagtaca aaagcctact
    12061 gcttatcttt agtgtatcat agaacttttt ttgatacatg caattatcta ccagctgaga
    12121 ttctcctcgc tgtgagaagt ttctgtgctt tcattgtatc gaaggatctc ttcggcaatc
    12181 tctagcagct gatcagaaac cagattggtt cgttggatga aataaaaata tcgatggaaa
    12241 ttcaacggag tcattggcac attttctgga acagagcgct tagacaaaat cgagcgaccg
    12301 attccttcct tcagcaacgc aacaattaca tcgttacttt tcacgatcat cttcttatct
    12361 tggatattca tctcttcaaa ataacgctgc gtgtaatgaa aaacaccaga gtttgcttct
    12421 cttacaagcc acaatttgct ttttgggtcc ccagcgatga ctagctggtc ttccatcaaa
    12481 gagtatcttt gtacgccgga agtctctaat ggcttttcga taaaaccaaa atcggattca
    12541 tgtttcgcta cttgttctaa tgattcatga gaattcgtga tttcgatctc aaaatgcact
    12601 ttaggaaacc ttttgatcag atgtttcatc aactgaggca aaagatagac agcaaaggtg
    12661 tgggaagcaa cgatccgaca tacttcttct tcattttgtt cattgagcaa tttttgctga
    12721 gtttcttccc attcttctaa caaaaccagt gtccgttcat ataataagtc ggcttgctta
    12781 gttggaagca tctcttttct tccgttacgt tgaaaaagat cgatattcaa ttcccgctcc
    12841 aactgtttga tttgattcga gactgctggt tgagataaaa acagtacttc tgctgctctg
    12901 gaaaaatttc ttgtttcata tactgctcga aaagtcgtta ataacttaaa catggcacca
    12961 cttccataac tttttatgat aattactata aaaactattt gatttaaata tacaaaaaac
    13021 catctataat agaaatgtac aacaaaacag ttgctgatcc aactagtaac ttagatgaag
    13081 acaatcagct gtctgcttgc tcgtaatcat acattcctat tcattttaca atgtattcgt
    13141 tcatcttggt actaaaatct attaaataaa aaggagaata ttatatatga agaacttttt
    13201 gaaaattatt ccgatcccga tctgcgggtt gatccttggg ttagcttccc ttggaaatct
    13261 gtttaaagac tatcaccatg ttgctttagg caatctaact ggcggtatct ctatgattct
    13321 tatgatattc atttttgtta aattgtttat cttattcgaa cacacgaaac aaacgttgca
    13381 tgatccaatc atcgcctccg tttcaccaac ttttacgatg tcactcatgg ttatttgtac
    13441 ttattttgtt tcatatgaaa gtattgcacc aattgtcaaa ttcatttggc tgtgcgctgt
    13501 tatcttccaa gtgatccttg tcttttattt caattatcac catgttatca aagctgatct
    13561 ttcaattgaa gcaatttatc caagctggtt tatcatttac gttggatttg gggtcatcac
    13621 tgtaactgcc gggaacttct tcccacttat tggaaaaatt ttcttttgga tttcattgat
    13681 ctgctatgcg ttcttattgc cgatcatcat caaacgtgtc tttttcgtaa gaaatatggc
    13741 tcatccgaca cgtccgctga ttacgatcgt tgcagcacct ggttctcttt gcctaacagg
    13801 ttatcttaaa aactttgctg aacctaattt ttatcttgtc gtagcattgt tcgctttatc
    13861 tcaagtattg tatttcgttg tgctgtctat gcttccacgt ttgttcaagc ttcgttttta
    13921 tccaagctat gcagccttta cattcccgct tgttatctct gcaacagcta tatttactac
    13981 tgttcattac ttcaacagtt taggtatcta tagtagcgta ttggatttct taaaagttgc
    14041 ggaatgtctg tttgcaagtg tagttgtctt ctacgttctt gctcattacg tacgtttctt
    14101 aattaaagaa catagcaaac atggaaaatt tgtttctaaa aaaatcgctt aataatcgat
    14161 tcaataggtt gtgaaaaagc tgttctgcat caagtaataa gaacggccaa acaaaaacag
    14221 ctgttcatgt ttttgtactg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt
    14281 ttaccaagag gtcgtaaaag aagtggtcag cttcaagaaa taagaaggaa tttacgaaaa
    14341 ttgcttctca aatttttgtg aaatttcggc ttatttccgt agaagctact tctggaacac
    14401 cgtttattcg aaacaagagg tcgtacaaga cttttgtcac gacctcttgt tcttttgtca
    14461 aaatactggt ttagtgctac tatgttcgag tatgctttta gtatttattg acgaaaggaa
    14521 ccgatcaaat gaaatcattg tcacatccaa taaaatctgt aaaggattca gcggtccgcg
    14581 aaatcttacc cggtttactg ctttcttttg ccatcgctgc cttcagcagg cttcttgcta
    14641 tttatctgcc acagttaggt ggagcgacta ttgctatttt attgggtatt ttattaggaa
    14701 atactttttt taaacagcct gttctagcaa tcggcacaaa atttgctgaa agcaaactat
    14761 tagaattctc tgttttttta ctaggtgcaa ctgtcacagc tcaaacgatt gctcaaattg
    14821 gtgtaaaagg aattgttttt gtgttactgc aaatgtcagt cactatcatc ggtgcttact
    14881 tgattggtaa aaaactgcaa ttcagcgata cttcttcttt gatgatggct ggaggaaacg
    14941 ctgtttgtgg ttcttctgct attgcttcaa tcgccccttc catccatgcg aaagaggaag
    15001 aaaaaggaca gatcatcaca ctagtcaatc tactgggtac tgtgatgatg ttgactctcc
    15061 cttttcttgg attacagttg tttggtggaa atctcattga aaaaagtgca cttttgggtg
    15121 gtacactcca atctgttggt caagtagtcg ctggggcaag cttacttgac aattctgttg
    15181 tcgaattcgc catgctattt aagattctac gtattatgat gctcgttgtt gttgtctatc
    15241 tgtttgaaaa attcatgcac aagaaacaac cagctctgtt tgaaacgcca actgatacgc
    15301 agaaaaaacg aaaattaccg cttccttggt atgtattagg gtttgtgatc ttttgtttgg
    15361 tcaacagctt gttttcttta cctgtagtta ttgaccaatc ggcacatttt atcagcagct
    15421 ggtttgagat cactgcttta gcggcaattg gtctgcgttt agactttgca aaattcatga
    15481 aagaaggtac acgcttctta tcttatggat tagctgtcgg gcttctccaa acagtgatgg
    15541 ctttactatt tatttggctg atcaaagttt agaatgaaca tgaaaaaaca gttgtgattc
    15601 tcgcatggaa caatgtccga atcacaactg tttttaattt tgcagatatc cttctaacaa
    15661 caacaattgg cgtttgattt caaccccatc tattgcgttt cccatatatc ctgtcagttt
    15721 tccattttgc ccgatcactc gatgacaagg aataacgatt ggcaatggat tggttcgatt
    15781 tgcctgccca atcgctcgca cagctttcgg actatcaaca gcaacggcta cttctttata
    15841 cgttcggatt tctccataag gaatggtcga caaagctttc cagacatttt gctgaaaagg
    15901 cgtacctctc tttatagata aaggaacatc aaattctttt ctttttcccg caaaatattc
    15961 ttcaaactgt tcttgtgtgc gcgttaaaat tcgctgattc gctgattcgc tttctgaaat
    16021 tggctgaaaa gatacagcag tcaatccgga ttcatctgca tctaaccata aaaagccgat
    16081 aggtgtgtgt attttcattt tgtttgctcc tagtcaatag actggaactt cctccttttt
    16141 ctctatccaa aactacttgc caactaataa aaattgttct taaaagtttg ataaaccgag
    16201 gaaacatggt aaactaaata cgttagttta caaaaagaat acctgttctt ttacgtaaaa
    16261 tcaaatttta aaggtgttat ttaaggttta ttaaattttg aatgtaaacc atatataaac
    16321 atacgattga aaggatagaa acgatgaaag tatcaattgt tgtcccatgt tttaatgaag
    16381 aaaaaactgt tcctcttttt tttgatgagg ttgaaaaatt caaaggtgcc catgagttcg
    16441 aatatttgtt tattgacgac ggatcaaaag acggtacgtt agcagcaatc aagaagctag
    16501 cctcagctta tccttatgtc cgctttgtct cattttccag aaattttggg aaagaagcgg
    16561 ctctgtacgc tggattgcaa gcttctacag gagatttagt gaccgtgatg gatgttgatc
    16621 tgcaagatcc gcccgaactg cttcctgaga tgatcaaccg gatcactact tctgatatcg
    16681 attgtgtcgg cacccggcgt tcgactcgtg aaggcgagcc tgcgatacgc agtttctttg
    16741 cgaaaaaatt ctaccagttg atcaataaaa tcagcgatac agaaatggtc gatggcgctc
    16801 gtgatttccg cttgatgact cgtcaaatgg tggatgccgt cctagaatta acagaataca
    16861 accgattttc aaaaggatta tttagctggg tcggatttaa aacagaatat atttcttatg
    16921 aaaaccgcga acgtgtggct ggcgaaacat cttggtcctt ctggaaatta ttcaactact
    16981 cgattgatgg tatcgtgaac ttctcggatg caccgttaac gattgcttca tttatcggcg
    17041 ctttgtcttg tgtcggttct ggtattgcaa ttttgttcat tattttgcgg gcattgttgt
    17101 ttggtgatcc tacatcagga tggccttcca tggtctcgat cttcctgttc attggtggaa
    17161 tccaattgtt atgtcttgga atcatcggga aatatattgg gaaaatcttc ttagaaacga
    17221 aacagcgtcc tgtttatatc ataagagaaa cagaaaaagt gaagccaaat gaattgaagc
    17281 ctcaccaata atatacgtca aatgaagtaa acattttttt aaatgaaaaa ggactgtgac
    17341 aaaagtcttg tcacagtcct tttttccgaa taaatggtgt tccagaagta gcttcttcgg
    17401 aaataagccg aaatttcaca aaaatttgag aagcaatttt cgtaaattcc ttcttatttc
    17461 ttgaagctga ccacttctat cacaacctct ttcatatctt attacttctt cattgctgct
    17521 tttagtgctg ctgctaatgg gctttcttct ggttcttctg cttgtgcata ttttttcagc
    17581 aagcgtcgtt cttcatgttt ggtcattttc ttcttctgct cttttttatc aagcaatttt
    17641 tctgtcgttc catcgtattt acaacggaaa taagcaccgt ttttaccttc gatgatttcc
    17701 attttccggt gacattgcgg gcatcgatga ttagagactt ttggatcttt tcttctgcgg
    17761 tagttgcaat ctgaattggt gcagacatag attttgccgt cacgtgtgtt tttctcgcgc
    17821 agattggccc cacattctgg gcatttcttt tgggtaatcg agaaatcttg atattcttgt
    17881 ttgcttgatt tgatctcatt caccaagcgt tttgtttctt tttcgattcc attcaagaag
    17941 gttcctgctt gtttctttcc tgcagcaata gcttcaaggt ctttttccca gctttctgtc
    18001 aaatctggtg ttactaaaga gggatttacc aatactaata attgcttacc tttaggagat
    18061 acttgcagtc cttgaggggt acgttccatc agttcagatt tgattagttt ctcgataatc
    18121 tctgcacgcg tagctggggt tcccagactg tatttttcca tttgaccaag caagcttgct
    18181 tctgacaatg gtttaggcgg tgcagatagt tcttttttga tggcgaaatc gggattgatt
    18241 ctcattcctt tttcccattt gactgtttcc gtctcagttt cagtttcgcc tttccatcct
    18301 gcttggacga cacgattttg ccgaaaaatg aatcgttctt taccaaattc aaccgtaact
    18361 tttgtttggc tgactttatg gggttgagca aacagtccta aaaaacgaga gacaatcatt
    18421 tgatagattt ttcgctcttc attacttaat ttttcataac gtgctctgtt ttcagttggc
    18481 aaaagtgcat ggtgatcggt cactttactg tcttggaata cttttgtctg ttggacgatg
    18541 gctccgtttt tcaaatactg cttgacctca ggagcaaagt cgctgactgc ttgcaatcgt
    18601 tctttcatcg tacttttcat gtcatttgtc agatatttgc tgtctgtacg cggataagta
    18661 acgatcttat gggtctcata caaactttgt acaagactta atgttttctt tgcagaatag
    18721 ccatagcgtt gattggcttc tctttggatc tctgttaaat cataaggcaa tggcgcagat
    18781 tccgttttta atttttcttg gacttctgtg acagttcctt tgcttgtact gatcaattga
    18841 acaagttttt gtgcttcttc ttgtgttttt aaagcaaatt gatttttttg aagcattttg
    18901 gcttgttctt ttccagcttg caacgtgatc gtgaaatacg tttgtggacg gaactgctcg
    18961 attttctttt cttggtctct cactaatgct agtgtcggtg tctgcacacg gcctgctgat
    19021 aaattatccg cgtacttcac agttaaggct ctggtcacat tcaatcctac cagccagtcc
    19081 gcttttgccc gggcaagagc agattcatac aatgcatcat attctttcgc aggtcttagt
    19141 tgcttgaatc cagttttgat tgctttatct gtttgagaag agatccataa ccgcttcact
    19201 ggtttattga aatgcacata ctctaagatc cagcgagcaa caagttcccc ttctcttccg
    19261 gcatctgtcg caatgactgc ttctgagaca tcttttcgct gagccagctg cttgattgct
    19321 tttaattgat gtcctgtttt aggaagtggt ttgattccta gttttttagg gatcattggc
    19381 aacgtgtcca tttgccagct ctgccattcc ttattcagat cttccggcat tttcaatcct
    19441 aataaatgac caagggccca agtaacgatc accttgggtc cttcataata atttttgtgc
    19501 ttttgattag caccaagcac cttgcttagg tctttcgcaa cgcttggttt ttccgctaga
    19561 attagttgtt tcataaaatt cctttctgct ttctactgtg atgggctgat gatttttcaa
    19621 caacgcaagt cccttgtatt gctgtaaatc atcgtcacac tcttcacacc agcgctcatc
    19681 tccgtcattc caaaaggcgc tgagaaagtt actttttgca ttgatatatt cataattttg
    19741 atggatttcg ttccattgtt tttcgtacgc ttgtcgtgct tcttcgaaag actcgaatgt
    19801 ttcttcttct tcaatatctt cttcccagtc ttcaaaaaac caccaaggct cattgtcccc
    19861 gaacatggta attacttgat acatactcgt gctccttctt tcagcttttc tagactcatc
    19921 ttagtcactt ttcaagaaag ttgcaagaat tttctcttat aaaagggtca tttaatgaac
    19981 atcttaaaat tgttacaaat gaatctgatt gtttaaggca aagtagcgat aaagttattg
    20041 atcatccaaa aatagctgtc ttctggatcg attgttggtt cttttgtaaa gtatcctaaa
    20101 aaacgtaagg aaagataacc gtggatcata ctacgaagtt ccctgcttcg atgaactttt
    20161 tcttctttcg ttaattgaaa agcctcaaat atctgcaaaa tgatctgatt cgtttcatgg
    20221 attccatcta ataaaacagc ttcattggta tgcggtacac tgatcagtaa ctcatacact
    20281 gcttgatttg aaaaagcaaa ggatcgataa gtttctgcat agatctgcag tgcttcctga
    20341 ccactttttc ccacaagagc ccgtcgcagc tggtcattaa gttcctgcag aagtttcgct
    20401 gttagcgctg ttttgacatc acgaatattt ttgaaatggt tatacagcga gggagccttg
    20461 atatccaagc gtttggctaa atgctgaaag gttatttgct gaacatccat ctcttctgct
    20521 agttctcgaa agcagtcgat gattttctct tgagacagat ttcttttttt catttccatg
    20581 tttcctctca taaaataaat ccttcactct ttcagtttac cacgaaaaag aaagagaaaa
    20641 aagagacttt cattgataaa ctacatattg tagtttataa taaagagctg tagatgaaaa
    20701 ttttcagaaa gaaggagtct aattggctgc aagaataaag gaaaaattat ttaggaaaag
    20761 agaaacttgg gaacaaaact tgtttgtctt atggtttgga acatttatgg ctggtatcgg
    20821 atttagttta gtcatgcctt ttatgtcact ttacatagat acgttaggta attatagtac
    20881 gtcacaattg aacttttgga gcggattgac tttttcatct actttcttgg tgactacgct
    20941 tatctctcct tggtggggaa aattagccga ccaaaaagga agaaaattga tgctgttgcg
    21001 tgcttcatta ggaatggcaa tcgtcatttc tttgatgggt tgcgtgacta gtgtgtatca
    21061 attagtcgca ttgcgtctgc ttcaagggat tttttctggt tatatcagta atgccactgc
    21121 ccttgtcgca actgggacgc caaaagaaaa aagtggtcaa gttcttggaa ccttagcaac
    21181 tggttctgtt acaggaacat tacttggacc gcttcttggc gggatctctg cttcagcgtt
    21241 tggctatcgc cctacctttt tcattacagg gatcatctta tttttcgtct tcttgttgag
    21301 tctgtttttc gtccatgaaa aatttgtccc agtgaaaaaa gaaaacatgg tttctgccaa
    21361 acagattttc aaagacttga aatatccaca cgtcgtcatc ggtatgttcg tcacaacgat
    21421 gatcatccaa gcatccaaca attccattag tccaatcatc agtctgtaca ttcgccaatt
    21481 gctgcatgga catgggaatg ttacgttgat cagcggaatc attgcttcga tacctgggat
    21541 tgccactttg atagccgctc caagatttgg tcgcttaggt gacaagattg ggagtgaaag
    21601 aatacttgcg attggtttgg gttttgcgat tctggtttat atcccaatgg cattcgtcac
    21661 gaatgtttgg caattagctg ccttacgttt tttgattggt atctctgatg cttgtctgct
    21721 ccctgctgtc caagcattga tcacaaaata ttcgcctcat caagctgctg gacggatctt
    21781 cagttataac caatctttcc aagcaactgg taacgtagta gggcctatga tcggctccag
    21841 tgtttccagt gcatttggtt atcgcggcgt ctttctttcg acttctcttc ttgttttggt
    21901 caacttcttg cttgtacgac acaatacaca agagatccat cagcaagaaa ctgcagaaga
    21961 aaaagcttca ggagtctcca tagcttcagg gcactctcct tctcatagac aataagcgta
    22021 aatgaaaaac actgcttgaa cagttgtcac agactgtcct tgcagtgttt ttgtttaggc
    22081 ctgttttttc aagaactcag ataagaggaa taatcccttt tccaaggttt cttttttaca
    22141 acagtaacct agtcgggcat gcttcggtaa atcaaaggca cttcctggta ctaatagtac
    22201 tcctgtttct tctaataaat tgatacaaaa tgtctggtca tcttcttcga tggctaattt
    22261 aatgaaagaa gtagagacgt aattgggagc gacaagttca actttcggtt cattggctac
    22321 ccattgtttt aaaatcacaa ggttctctgt aatgatcttt ttgtttcgcg ccaatatttt
    22381 ttctttattc ttcaatgcat gtacagcaag atcatctgac aggactccgc cgcaaatcat
    22441 cgtgtagtct cgatactttc gaaaaacttc tgccaactca ggtccagtag ctgtccagcc
    22501 aatacgaata cctggaatgg agtaggtttt ggatagggaa ttggttgcta tacctttttc
    22561 atagacatct acgatcgaca agaattcacc tttatccgtt aaaggcgcat atacttcatc
    22621 tatcaataca tatgcatcta ctgtacgagc aatttctgcg attttttcta aagttttccg
    22681 atctagcaat gtgcctgtag gattgtttgc actattcaaa cagatgattt ttgtttcagg
    22741 tttgactaag gcttctagct gttctaaatc aaactgccaa tcttcttctt ccttcaaatg
    22801 aacaaagtca acagcggcac ctaaagattt ggggatgtca tataattgct gataagtagg
    22861 aagcatcgaa acaacatggt ctcctgcttc cactaacgcg tatagagcta aaagattagc
    22921 accagtcgct ccattcgttt gtaagatgtt ttccggatcg acggtttgat aaagagaagc
    22981 gacttcttgt ttgaaaacat ctgatccttc gatccagcca tagtccaatg gttcgttttt
    23041 gcgtttttcg aaaaatttac ttacgctagt cccatctaag ccaacgagtt cgtctaatgt
    23101 caatgcttcg atcgagcttt gtgcaatatc atataccgct ttcttttccc atttatttag
    23161 ccattcttct acaccgaaat ttgcgatttt catgtttctt tcccccttat atcttgatcc
    23221 agcctacagc aagcaacccg attgaaagca gtgcggccac acaaatcagc ccaatcatta
    23281 ctttttcttt tcccgtaatt tgatgtttgg tttcacgaca agcgatccag tagaagcaaa
    23341 aaccaggaag ataggcaata ctacataaaa ggacataaga aaatcctgct aaaatgatgc
    23401 ccatcaattc aaaaactaca gcaatcccac cgatcactac ttgttttttg attccttctt
    23461 ttaaagaaaa ctggacttga tagatcccga caagtagata acaaatcaaa attgccgctg
    23521 tacataaaga ataggcaaat tgataggcat aatcagtaac taagaaagtc aataagaata
    23581 gattagtcaa gattgcagta acaactaacg aataagttgg ggcatttttt ttattcgttt
    23641 ttccccaacc tttcggcaac agaccgtctc gagccatcaa catcgttgtt tctgctggga
    23701 gcatcgtcca agacaaccaa gaaccaatga tcgaaataat caagccgata ttgatcagcg
    23761 ctgctcccca atttcccacg attcctttta aaatatttgc catagcaggt tgtgaaatag
    23821 ttgctaactc ttcttgagat aagacgccgt atggcaagat cgaagcaagg atatagataa
    23881 acaataaaca aagcagacca atgatcgatg cttgctgggc ttctgaacgt ttcttcgcgc
    23941 ggttggctaa aacacttgcg ccttcgatcc ctacaaagac ccacatcata accatcaaac
    24001 aacctttaat ctgcaaccag atcgttcctt gctgccctgt tccattcact agattatcag
    24061 atacgttacc ccaaaaatca gcagtgaata tattcacttt aaatgcgata aatacaaata
    24121 ttaaaaatag gaataatggc actaatttac agatcgtcac gattgtattg atgacgctcg
    24181 ctccttcaac tccattgttt actaataaag tcagacccca aataaaaata cttgcaaata
    24241 aaatagaagg aacattttgt ccacctttga atatcgggaa aaattctccc atggcgctca
    24301 tcagcatcgt tgcaaaagca acatttccca gccacgcaga caaccagtaa ccccaaccac
    24361 tgatgaagcc acctaattta ccaaatgaag cttccgcgta accgaaaata ccaccatcta
    24421 gatctggacg cttttctcct aaatgattca gcgataatac caaagctaaa ataccaatcc
    24481 caacgattat ccatgaaagg atcgctggcc ctggagctgc agaactagcc agatcacttg
    24541 tgatcccaaa cacaccgctt ccgattgacg aactgacaac cagagcaacc agtccccatt
    24601 tgctaatacc cgtcgatttc ttttccattt ccatcaccct cacttataaa attgaacaaa
    24661 tcacttgcaa aagattccct tctattttgc aactgctctt gcttaacaaa aagcaacttt
    24721 attcatatta ttggatatta tgaacttttt acacaagtat ttcaagtata aaatgaataa
    24781 aaattaattt tattgcaaaa aaacagctat gctatccaaa agaaaaggtg tttcttttgt
    24841 tagccatagc tgttttgatt catttaatat cgcttaaacg tattgtaaaa cttttgtttc
    24901 ttgatatgcg tggtcgatca aaccaccacc cagacattcc attccatcat aaaatacaac
    24961 tgcttgtcca ggagtgattg cacgtacagg ttcgtcgaat acgacttctg cacgattatc
    25021 atccaataag cgaactgtca caggaacgtc ttgctgacga taacggaatt tcgctgtaca
    25081 cttgaattct tttggcattg gttcattcgt cgtaaagtgg atttcacttg catccaagct
    25141 tgtggcatac aatgcaggat gatggaaccc ttgccccaca tataatgtgt ttgtcgctag
    25201 atcttttcct acaacaaacc agggttcttg cgaatctcct ccgccaccga tccctagtcc
    25261 ttgacgttga ccaatcgtat aatacatcaa gccagcatgt tgtcctttga cttcaccatc
    25321 aagagtgacc atatttcctt tttttgctgg cagatagttg ctcaaaaatt gtttgaagtt
    25381 cttttcacca ataaagcaga ttcccgttga atcttttttc ttagcagtcg caaggcctgc
    25441 gcgttcagca attgcccgta cttctgattt ttccatgccg cctagaggga acatcgtttt
    25501 tgaaagctgt tcttgtgata attggctaag gaagtaagtt tgatctttat tattgtctac
    25561 tccacgaagc atgtggacca cgccgttttc atcgcgagtg acttgcgcat aatgtccagt
    25621 agctacataa tcggcgccaa gatccattgc ataatccaag aatgctttga atttgatttc
    25681 tttgttgcac atcacgtctg ggttaggtgt acgtcctgcg cggtattcgg ctaggaaata
    25741 ttcaaataca cgatcccaat attctttttc aaaattcaca gagtaataag gaatcccgat
    25801 ttgagcagct actttcgcta catccttata atcttccgtg gccgtgcata caccgttttc
    25861 gtccgtgtca tcccaatttt tcatgaatat tccgatcacg tcgtatcctt gttcctttaa
    25921 caaaagtgct gttacagatg agtcgacacc gccgctcatc cctacgacaa cgcgtgtttt
    25981 gctgttgtct gtcatgatat caccatcatt tcaattagat tctgaaacat ctacgagttg
    26041 aaggtcgcaa cttttcagta ttttacatta tacgtcatat ttctgaaaat ttcatccctt
    26101 aattatcaag tcgtaaaaat actgttctac tggacctatt cagtacacaa acatgatagt
    26161 caaccaaaaa atcactcatc ttttgagcat tcgatataaa aaattggcat actcataaaa
    26221 agggccagcc tttcggcaat aaggaataat tttataaatt gtgaaaaact atttttcaaa
    26281 attgcttctt atcgcttaaa gctgcacgtc tcatttacaa ccttttcttt ttagatgtta
    26341 tgagttgctc agataagtga ttttgtttac tctaagaaaa aagggaggca aaagatacat
    26401 gaattacttt atcagtgata tgcacttttt ccatgaacgc ttattaggaa aaaacgactt
    26461 tgctccgcgg ctctttgcaa atgtggatga aatgaaccaa cagttgatca ctagctggaa
    26521 tgcagtaata aaagaaaccg accatgttta tcatctaggc gaccttgcta tgcatccgca
    26581 atatgaaaaa gcaaatgcag aaattttgga actggtcact caactgcaag gaacaatcca
    26641 cttgatcaaa gggaaccatg acagtcgcgc ttttttccgt tatttagaaa aaaatgatcc
    26701 taggcttact gacggaagtc ccaaatttta tttttatgat gtaggtgcca tcgttaaatt
    26761 cgaccatcaa caatattact tgactcatta tccgatgttg ctcgggccta acgacactat
    26821 ccgcaatctt catggacata tccatcatta cagcgtacct atcgagaacg atgtcaatgt
    26881 aggcgtagac gcaccggaaa gagaactctt aaaggaaaaa ctaccatttg gcactccgtt
    26941 atctgagcaa caaatcaaag aaatctatga agcaaagagg aagtacgata ggtcgtgaaa
    27001 aaactgccct atatcaagta ttaaacaaat aaagataatt actcatattt ttcatttgag
    27061 taggcttatt gccacaggaa cattaggatc agaaatggag ttgtaacaag acttctgtca
    27121 caactccatt ccatactatt cgtctttttg ctcatggtgt tcttttacca gttttttctg
    27181 taattcttta tcataatttc gggcagctga cgttaaaaat aaaacgattc cgcataataa
    27241 aatcccgatt ccggaaaaca agaacatttt ttctacccct aaacgatcag ctaatggtcc
    27301 tgcaaataat aatccaattg gacctgccag gttcatcaaa gagttgaagt tccctaagac
    27361 acgacctaaa acattcgatt cataactctg ctggatcatt gccatcagca gtgtattgaa
    27421 atatggcgtt gcacaacctg ctcctgcatt caagataaga aagtacaaga acccttgact
    27481 atcgtttgga accaatccgc ttgcaccgat cgtcaggccc ataatgatgt aggctataat
    27541 aataggtttc attcggtctt tccatgttcc tataaaactg atcagtgcac cgcctaagag
    27601 cattccgact gcgtagacta cttccactgc tcccgcctgt ccaacggaac caccaaagta
    27661 atctagcgtc atcaatggat acagactgat tgctggcatg aacaacagca tcacaaatgc
    27721 accattgatc gttatgtacc ataacccttt attatccatc aattgctgca gcccaaattt
    27781 tgcatcttta aaaatagtaa gcggaacttc caatatttca ggagaaactt ttgggatctt
    27841 cacaaaaatc aataagccga caccaaaaac tgcccccaat acatctaata aaataaggta
    27901 attgacagga acgactgaaa acaaagccgc acctagagct ggcgcaatga tgaaattcgc
    27961 tgattggacc attcctaact gtccattcgt ccttgttatg tgagaagatg gaacgattgt
    28021 cggcataatc gactggatcg ttggcatttg gaatgtttgt gcgaccgaac gcataaacaa
    28081 agacacaaaa actagccaaa tcgggaagga ttctgaaatc gttccgacta ctgctaaaat
    28141 aagcgcgaaa atcgctacaa taatatccgt gacaatcaaa agagcttttt tatcccagcg
    28201 atccactagc ggtcctacaa atgggcttag taaaaccatg ggaatcattc ctagtaaagt
    28261 agcaaaactc aaaattgtcg ccgaaccggt ttctcttgtc aaataccaga tgatcgcata
    28321 ttggacgacc atactagtga ttcctgacaa aaactgtccc gataaaaaga acaaataatt
    28381 tctcttccaa ttcgttaaaa cgaccgactg ttcattttcc ataaacatcc ctcttttctt
    28441 tttcattttg tatagcgttt gcacaacaat cattatacgg gattcttttt tcaaaaaata
    28501 cctttttccc taaataacaa tcgtttcaat ttaaatttct ttacttagaa acaataaatc
    28561 agcaaaatat taaaaataac aacatgtgtt aaatttaaaa ataaaaaaga caactaattt
    28621 gcgtcgtcct tacaaagtta gtcatctaac aataggccgt ttcaaaacta gttgtctttt
    28681 tatatccaca gatcattcga ctttttcgaa taacccacgg ctaatcatcc catccatcag
    28741 aaaataaaat ttatcttggc tcattttctg ttttggatct ttgaaaatat tgactgcttt
    28801 caatccattt gctgtagtga tcgacatttt caatgtttga atatcttttg atacagtgat
    28861 ttttaatttg aatccttctt tgctttgcgg cgtggcatct aatggtcgga tcaattgata
    28921 attgcctccg tttgtttctg taaatccatt atcgcgcaat gtatatcttt ttgcatcagg
    28981 atgcaaacga tagctaattg gtgcgcccag taccgttgct gttgtttcaa atgccatgat
    29041 tttcacctcg ataaaattag tttctcttta ttataaaagt ttatcctgat ttttactagg
    29101 tttttcagtt aagttcccta aaaaatgaag gcgttctctc agacatctgt tcatataaga
    29161 atgacttcac tagttatttt ttgacagcac gaactaattg atcaaggaaa tactggatgt
    29221 cgtcttcggt atttcccaaa ccaaagctga tacggatgga ttcttttagt atcggtgtat
    29281 cttctccata cattgcttct aatacgtgtg aaggctcgat atttcctgct gtacaagctg
    29341 aaccagtaga aatagcgacc ccattaagat caagcttcat cagtagcaaa tcatttgctt
    29401 cgccaattaa gcgaagattc aacacatgag ggagtttatt cgttaaatca ccatttactt
    29461 gataagggat ttcgtttgct tctaatgtat caagaatcaa ttgctggaat cctttatatt
    29521 tgttgttacg tgcttcacgt tcttcaggtg acagcagttc aactgctttt gccatacccc
    29581 agatccctgc taaattttct gtacctgcac gccgcttttc ttcctgctcc ccgcctaaaa
    29641 gcaatgcagg gatatttact ccgtctcgtt tatataagaa accgacgcct tttggcccat
    29701 tgattttatg agcagagata cttaataaat cgattcctaa ttcttctgga tggatcgttt
    29761 ggtttccgta cgcttgtacc gcatctgtat ggaaataagc tggatgatcc gctaaaagtt
    29821 caccgatttc tttgattggc atcaaatttc cgatttcatt gttcccgtac atcacagaga
    29881 ccaagatcgt atcttctcgt aacgcttttt tgacctcttc gatcgacaga ttcccttttt
    29941 catctacagg cagatatgta acgtcaaacc cctttgtctc taaatatttc atggtattaa
    30001 gaacagcagg atgttcgata gctgtagtga ttagatgtct gccttctttt tgacgcgaga
    30061 acgctgtttc taagatagct gtattatccc cttctgttcc cccactgtta aagatgatct
    30121 cgtgaggttt tgcttgcaga ctgtctgcaa tgatttgtct tgctgtttcc aatttttcat
    30181 gcgcgtggcg accaaatcca tgtatgcttg aaggatttcc aaacgtgtct tgcatgattg
    30241 ccgtcatcgc ttcaattact tgtggatgca tgggggtagt tgccgcatga tccagataaa
    30301 tagttttcac ttcattcatc ccttccaaat tattgctagg cacattctag cataaaatag
    30361 cgaacaaacc aaagaagagc tcatttttgt ctagaggacg tttcatgggc atagaaaaaa
    30421 ggagctgtga caaaagtctt gtcgcagctc cttgaagctg ctcacttctg tcacaacttc
    30481 tttccctctt ttttttgttt attcgaattc tttttcttcc agacggaata acggactcat
    30541 cggtgtattt tcatggatgc gtttgaccgc ttcacccatt aattctgaac atgtaacgat
    30601 tgttaagttt tctggatgac gattttcttc tgtgaaaaca gaatcagtga tacaaatatc
    30661 tttgattggt gcattgtcta aaatactttt cgcatgatcg gacaataatc catgtgacgc
    30721 acaggcatat acttcttttg cgccgttttc ttttaataca tttgctgctc gtgcaagcgt
    30781 tgctcctgtg ttcaagatat catctaccat gatacatgtc tttccttgaa catcaccgat
    30841 cacataacca ccctcagttg aagcatcatc tgcatgatct acgatggcta atgtgctgtt
    30901 caaatattct gaaagactac gtgcacgctg aacaccgctg ttttttggcg aaacaactac
    30961 gatgtcatct cccgtcagac ctaattgacg gtaataatga gcaaacaacg gcatcgtaaa
    31021 taaattatct acaggaatat caaagaatcc ttgtacttga accgtgtgca gatctaaagt
    31081 cagtactcga gttgcccctg cttcttcgat caagttagcg atcaatttcg cagtgatcgg
    31141 ttcatgaggt ttcgccgtgc ggtcttgcct agcataccca tagtaaggca atacgacatt
    31201 gatcgttttc gcacttgctc gcttcattgc atcgatcatg atcagtagtt ccatatagta
    31261 atcattgaca ggtgcattcg ttgattgaac gatatacaca tgatcccctc gaatactttc
    31321 ttcgatattg atggaaatct cgccatcact aaattgcttc acgacgcttt tacctaattc
    31381 tgttccgaac actttcgcga ttttttcagc taatggacgg ttgccattca aactaaaaat
    31441 cttcaacgtg tcatcctgat acttattact catgtagtcc tccccaaaac ttctctattc
    31501 ttcgatagtt taacatattt ttccaagatt ttctgttaat ttcagcaaat tcttaaggaa
    31561 gtgaaactgt tccgttactc caactggcag gaatgacttt atgataatcg ccccagttgt
    31621 cgtgtacttt gtaataacgt tctcctgttt cagtttcaaa ataagcgtac gctgtcaccc
    31681 aatgattgcc atatgtactg ccaagtactt tcaaaatacc aatcatcaca ggtttgcctt
    31741 cacgtatacg ttttgtagct cgctgccatg aacctagaag agttgctcgt gcacgctcat
    31801 gattgccatt cttctttaaa aaactagaaa tccctgtact aacctgaaaa ggaatcgtag
    31861 gaagtcctag tggttggatc tggctacgca atgccttcgt caaaaccaac gaatcagccg
    31921 tatttttctt gcgaatctca tttggaagca tccacccctt acgatagtct tgataatagg
    31981 caaggagaac tgctgccgcg taagtaccac aaagatagcc tgagggggta gcccatttct
    32041 tatacctcaa aaaacgttct tctggcaatc ccacccagca atttgcgagt tcctttcctg
    32101 accttttttc ttcgatcaaa acgggaatat cttcttttct gttgatccat ctagataact
    32161 cgattggctc ttcattgatc agcttccatt ttttacaaga aagaccctct tgataagtat
    32221 attttgctgg agaatattct accggatgtc ctgattgatc gataatcaca taactttgat
    32281 cttcaaaaac cagagctaaa aaaagcaatt tactttttct tgactgcagc tgctgagtgc
    32341 tggctaattt tttaattttt ttatctactt tgaaatataa aggtttatcc actacttgat
    32401 tcagttggga tcgcctcctt tttttcatta taagtgaagg attgaaaaaa acaaatcagt
    32461 ttgattgttc gaattttcaa ttttttatgt tctatttgtt aattccttca ttttgtaaga
    32521 taatgggcac acgaaacaaa aaaggagcga tcgatgaatg aacaaagaac gattgaaaca
    32581 agatttctat gaagcagtca atgaagaatg gcaaaaaaca gcaaaaatcc ctgcagataa
    32641 accagctaca ggcggtttcc aagatttagt tgacgggatc gaccaacttt tgatgggaga
    32701 aacagacgat ctgcttgccc atccagaaaa agtgtcttca gaaaaaatga aacatttcct
    32761 tgcatattat caactggcaa acgattatga ccgaagaaat gagttgcatg ctcaaccgct
    32821 tcaacctata atcgaaaaag ttgaatcatt gcagtctttc gacgaattga ataagcaatt
    32881 tccagaatgg ctgttggata cattaccatt gccatttaat ctggatgtag atgcagatat
    32941 gaagaatgcg caaacgaacg ccttctttgc ttatccgcct tctttattcc ttccagataa
    33001 gacgtattat gcatcagacc atcccaacgg tgcacaacta ataggcgttt tctttgatat
    33061 ggctgttcag ttattagagc ttgccggcaa agaaaaaagt caggcagaag caatcacgga
    33121 acaagcagtg caatttgata aattgattgc tcctcatgta aaaagtgcag aagaaaacgc
    33181 agattacagc aaaatgtata atccgcaatc ttttgataca tttattaaat acacagataa
    33241 actggacttg aaaaaacttg tcacgggttt gattggggca actccagata aagtgatcgt
    33301 aactgatcca gtgtacttcg atcatttgaa tgacatcctg acagaagctc attttccgtt
    33361 attcaaaagc tggatgatca ttaaaacagt acgttctctt agtggttatc tatcagaaga
    33421 attccgccaa gtcagcggaa tctattctcg cgtattgtct ggaacagacg aagcaatgcc
    33481 tcagaaaaaa gcagcgtatt accttgcctc tggacaattc gatcaagtag ttggcgatta
    33541 ctacggaaaa aaatattttg gcgaagctgc taaaaaagac gtagaacaaa tggtgaaaaa
    33601 aatgatcgcc gtttataaaa aacgtctaga agaaaatgac tggcttgggg atgcaacaaa
    33661 agaaaaagcc gtcataaaat taaataactt aggtatccaa gtaggctacc ccgataagat
    33721 cccagcatta ttcgaccagt tcaaaacagt acctgcagat cagggtggta ctttgctttc
    33781 aaatactctt gttttcagca aactcacatt aaaagatcgt ttcagcaaat ggaacaaacc
    33841 agtggatcgt tcagaatggg aaatgagtgc tgacacggtc aatgcttatt atcatccatt
    33901 tcggaatgtg atcgtctttc cggcagcgat ccttcaagca ccattttaca gtctagaaca
    33961 atcaagctct gctaattatg gtggaatcgg agctgttatc gctcatgaga tttcccatgc
    34021 atttgataat aacggcgctt tatttgacga atatggcaac ttgaacaact ggtggacaga
    34081 agaagatctt acacaatttc aaaacaaagc acaagcaatg atcgaagaat tcgatggtct
    34141 gccttttgct gacggaaaag taaacggaaa attaaccgtt tcggaaaata ttgctgatgc
    34201 aggcggtcta agctgtgcat tggaagcagc aaaaacggaa tcagatcttt ctatcgagga
    34261 attctttgtc aattgggcaa cgatttggcg tacaaaagca aaaaaagaat accaacaact
    34321 tttactgcaa atcgatgtcc atgcccctgc taaactccga gcaaacgtgc aaccaaaaaa
    34381 ccttgacgaa ttctatgaaa catttggtat cacggaacaa gacgctatgt atctagcgcc
    34441 agaaaaaaga gtgcgaatct ggtaagaggt tgtgccagaa gtggtcagct tcaagaaata
    34501 agaaggaatt tgggaaaatc gcttctcaaa tttttgtgac atttcggctt atttttcgaa
    34561 gaagttactt ctggaatacc atgtattaga ggttgtgaaa aagctgctta gtttcaagta
    34621 gtaagaaaaa ttttgtgaaa atggctttcc acatttttgc ataaatttga cttactaccg
    34681 agaaactggc ttttgaacac cgtttatcga gaggttgtga aatcagcgtc ctgacctgag
    34741 tattagagaa aaaaacggaa aaatagttcc tcatattttg ccgtttgttg agctaatacc
    34801 gagggtcagc tgattgaaca ccgtttagtg gagagattgt gaaaaagctg tcctgcatca
    34861 agtaataaga acagccaatc aaaagcagca aggagaacct catctataaa aagatagaag
    34921 gaagtgagac atctattggt ctcatttcct tctattttta ctttcctcag tcaattgttt
    34981 tacatcataa aaggagaaat tccacttatt tttttcgctg tttcatgaca taaacaacta
    35041 caccgattaa caaaattcct ccagctaatg ctagcaacgg gttattaaat tctcccgttt
    35101 ttgggagtcc ttgagtgcca tttttttcac tttcttttgc tgtcctatcc ggatgagaag
    35161 gttttccatt tggttttcca tttggtgatt catttcctgg attttctctt tctggtttgt
    35221 catttcccgg gggcacatcg gcagtttctc gcacagtgat cgctttttgc tgggtaacag
    35281 attctgtatg agcagaccat ttttccccat caaatatttc cgaacgataa gtgacttcta
    35341 gcgtatattc tccttttgaa ggtgctgaaa aaatagcggt atgattagaa aattgagcac
    35401 ttgactgtgt ttcaacgatt tggatctttt ctgggaaata acgcatatct tcaaagacag
    35461 gcgttccttg atttggtgtg aaagttgaca caaaaactgt aaaaggcttc agttcaatga
    35521 tatttccttc tgcattctcc gccacaccat ttgcttcttg tataataatc ggattatttt
    35581 gttcatttgg ttttgtctga tttggttctt gtacgattgc atactctgga tattccgaag
    35641 acggtgaggt ttctccgtca aaaatagcac ctacagataa tggaagacca tccactcttt
    35701 ccttttctgt ttgacgattc cccggtttaa tctgcatcag tccatgaaca tggatacttc
    35761 cattttcata gcgcgtaaca ggctgctgac cttctttcga acatgtattt agagactcga
    35821 tccaatcttt tgatatggta tgtccaagag tattttctga ttgtttctct cgttcattcc
    35881 aatagtagtt caacgagtgg tagatttgat cttcgttttg attcaatggg cggatctgct
    35941 ctctcatctc aagattctct gttcggaaag ataagatacc agaagcatgg tagtctgtgt
    36001 ttcctgctga agaggtataa agagccacat tatatgaacc gttattaaaa cttgtactgc
    36061 tattgacgat aatatctgtt ccgctattgg aatcgattcc tttcgcaaga ttttcgaaag
    36121 acaaactatt gatcaattca tgagggcccg ttaaagaaga accgcccatt ttgaatccat
    36181 taccgttacc ttctccctca attcctggta cccagccgtt tcgataagca actgagtttt
    36241 tgatgattac ttttccgatc gaacctgttt catttttagc aaaaaggtcc caaccgtcat
    36301 ctgcattatg atacgcgata caaccgtcaa atacatttcc ttctcctatt gtcagtttgg
    36361 ccgcaaatcc atcggcatct tcaaatcctg gatcggcatt ttcaaaagat gtacagttct
    36421 tgactaagtt atgcgcgggc catgcttcga aaggatcatt tgagctaccg ctgatttgga
    36481 ttcctgtatt tccattccga taagcatgaa tctcttcgat caaattatga tttccagaaa
    36541 tctgtaatcc tttttgcata gctgcactat ttgtaatgtc aaaccctcgt aaaccccagt
    36601 agtgactgaa cagtgtcatc ccatttccag ttcctttaaa atcaagtaca ggacgcttgt
    36661 tttctggatt tttctctgca atcaactgaa tcgggttttc tcttgtcccg ttaacgcctt
    36721 ttggaatcgt caatcctctt tcgaaggaat aagttccagg cgccatgacg attgtctgcc
    36781 cagcatatgc gtatctcaat gcttgtgtca gagataatgg attttgtctg cttccgtttc
    36841 ctgcatctga cccttctggt gttacataaa ctgtcgaata tttaggttta cggtagtcca
    36901 ctgtatgaag aatctctttt gtttcataac tagtcatctc tacgtattct tctggcggat
    36961 agtttttatc tggcgtaaaa ctgaaagtaa attcgttcgt tccttccatc agttttgtct
    37021 gataatcaaa ttcccgattc gcagtgatag ccaagttttt tgcgttctta agcacctctc
    37081 ctgttgcttt atttttaatt gtcaataatc cgtcagcatt tgctctaaag gacatagtat
    37141 gatcttggtt tcctgaatga ttactagagg tcacagtgta aatcggatca atcaagcgag
    37201 taggtctttc ttgcccagga ggatctgtcg caggatctga gtaagcaact tcgattcctt
    37261 ctaccttgat cgtcatattc cttgctgcag caaatcctgc ataaataacc gattcatctg
    37321 aagcgtataa tttttcccag tcatacatga tgtcagaagc catttcttct cctgtttcag
    37381 gatcgtaata atatgcctct aaaccagtat tcgttttttt catctctacg tccagtttag
    37441 taatcgagtt cgtggccgga aacagattcc catgaccgtt tccgaatata ttataagaac
    37501 ctgtttcttt ttccaagaac cctgctgaag tctctaaagg tgttgtttcc actgagacac
    37561 tcccaggagc tggcgcagct tgcggatctt cagatgatat tcctgtgatt gcccgtgtcc
    37621 ccaatccaag tcgcatcgta tatttgctgc cgctcgtgtt gaccacttct tgtctatccg
    37681 agtcccagac atattcgatc cgactgccta gtatggcaaa tgaatttgaa taaaatgcct
    37741 gcccaaacaa atgatttttt ggtactgcgt ctcttaccat taaagcaaat ccttcttggg
    37801 cattcgtata ggtccaagat tctatttcga tcgtcgcccg catcctaaag ttgatgtctt
    37861 tggggattgc ttgataataa taggtcattc catctgatcc gcttgactgg aattttccgc
    37921 cgttattcgt ggaagagagc cgcacgccgt cttctaatgg ttcaatggca tacctactag
    37981 cacttgtgct ttctccgaaa acaacccctt tccactcttc atttgcttct tcagattgct
    38041 tttttgtctt ttcattttct gattctttca tcataggatc atttccttct tccgtatcca
    38101 tatcttcact agttcttggt atttcctcca ttgttgacat cccttcaatg ttcacgtgtg
    38161 cattttcttc gttcacagac tgttgttcgt tagcagcaac ttcaacgaca gaaattaaaa
    38221 ccggggtact gagcataagg agattcatca ttagcgcact gtattttata cgttttttta
    38281 acaagtgtat tcctcccgta acaaattaga aagcgtttac ttaaagtatt acctaattta
    38341 tttttactgt caataaaaaa caaaattata ataagtgtat tttataaaaa gaaaaaatat
    38401 aacttaagtt gttgataata aagagattta ttaacgtaaa aagaataagt ttttttaaaa
    38461 taacgtataa aaaagagcat aaaacaaagt cgtttgtttt atgctcttct tttattattt
    38521 tgtgcttttt attttatcaa atcactttgt ttttttataa aatttctgcg cctagtgtgg
    38581 actcaaaatg tctcaatgcc cattcatgtc caacgggatc gaaacttgcc acggaatctt
    38641 tgaaaatatg gagtttgtat cctagattat acgcatcgac tgctgtatgc aacacacaga
    38701 tatctgtaca tacgccggtc aaataaatat ctgtgatctg gcgttctcgc aaacggatgt
    38761 ctagatctgt tccactaaaa gcggaataat gccgtttgtc tatccaataa acattttcct
    38821 cttgctcata gtcttggtat aaacttttta aagagccgta caattcccgt ccacccgttc
    38881 ctatcacatt atgcggagga aataagcggt tctccggatg aaaggaatct tttggatcat
    38941 gcgcatcaat ggcaaaaacg acaaaatcct tttgatcaat aaacttcttt gtatgagcag
    39001 tcagcttttt ttcaattact tgtcctgctg tcccagtagt cagctttcca tcctctgcaa
    39061 caaaatcata tgtgtagtca atcgagatca atgcgttcat cttagtcctc cttatacgag
    39121 atgtttgaca aattcgatca ccatttctgc tgatcgtttg cctgcgtctt cgataaattc
    39181 gtcaaagctt tgtgtcgccg agtgatctgc tgtgtcgctc attgcacgta cgatcaaaaa
    39241 tgggatattg aactgacgag ctgtttgtcc aacagcggct ccttccattt cgcaagctaa
    39301 tgcttcaggg aaattcgtta agatttcttt gattttatct ggcgagtcga caaatgtatc
    39361 acctgtaacg atcaatcctt cttttgcgtt caaattcgtt gcttttgctg ctttaaccat
    39421 ttcactgcgc aaatatgtgc ttgcttcata atatagaggc attcctggta attgtcctgg
    39481 gttgtagcca aatcctgttg catctgcatc aaaataagct actttgtctg atataacgat
    39541 gtcacctact tgtaagcctt caccgattcc gccagctgaa cctgtattga tcaccatgtt
    39601 cacgccgtat tgttggatca acagacttgt ggtaatagat gctaagactt ttcctatacc
    39661 ggaacgtacg acgatcactt cgtggttacc taatgaacct gaaataaata aagcgcctgc
    39721 gcgttcccat gataatggtt cgcttaggtt ctctctcaaa atttttactt cttcttccat
    39781 tgcgccgata ataccgattt tcatcttttt aattccttct ttctatataa atgcgattgc
    39841 taaaaaaacc acacataaca ttaggataac aataataaga gatttcatca aaaaagagtt
    39901 ccatttggtc gtcttttccc gttcgctgat ccttgttttc gtgattggtt tattcttttt
    39961 gctttcttta cgataaaagc tggcaatctt tttttcttct tgctgataag ctgtttctgc
    40021 ttttcgctgt ttttttaatg actcgcttgc ttgtgcttgt tgtcgcttgc gaagttcgct
    40081 tcgcgtaata agcggtcctt tggccataag ccgatccctc tactttcctt tggtcaatag
    40141 ttcccagtat tgaatcgcat aaatcgtctt tgcatcacaa atcaactgat ctttcatcgc
    40201 ttgttgcgcc tcttcaagag ttagatgata caactccagt acttcgtctt catcttgtgc
    40261 taaaggattc tcgaccttct ctaccccttg tgcatgataa atatgaagga cttcatttgc
    40321 aaaacctggt gaaagataca tcgaagtaag atggctcagc gacttcgcac gatagccagt
    40381 ttcttcttct aattctctag ctgctgtcat ttcgggattt tgtccttctc ctggatcgat
    40441 ttttccagct ggaatctcca aaatcacttt ttctaaaggt tttcgaaatt gtttgacaag
    40501 aagcagacga tcttgttcgt caaaagcaat gatcccgaca ccgccaggat gaaataccag
    40561 ttctctcttc gaagttcctc catctggcaa acgaacttcg tctacagcaa catcaataat
    40621 ttttccgcta tagatttctt tgcgactgat tgttttttct tcaaattgct cattgttcag
    40681 catatcttct cctgctttct ttgtaatcat aactgctttt ttttcatttt tcaatttttc
    40741 tcaaaaactc cttattagtt taccatagat aaaccttaga ttttctctaa atatagaaaa
    40801 tccttcttta gtccgatact ctttctagta gtccgtatgc taaaataagt atgtaatcga
    40861 ataacgaaaa gaattcaata ctcaacgata aatgacatgg agggtttatt atgataaaac
    40921 gaaattggcc gtggcttcta gcattggtct tgctatttct ctttgtccga aacaatagtt
    40981 tttcttttgt tctaggactt ttattgatag gtgtagtttt agttttgtgg ggactctttg
    41041 gttcgcgcag gaaaaaggga aaacaagaag aaattcctgc actgactgac gaacttgaaa
    41101 ctcactatga aaaaagcggt atgacagcga atgaaatttc attttttcga caaacaatga
    41161 atcaaacaaa aaatgagatc aatcaattgc aaatgaatat gcagcagaca gccaaactta
    41221 aagcaatcga tctccgccat gatccagtca aagcagcgaa agctttattc aaagaattgg
    41281 tgaaagaacc aaaccgactg catgaagcaa gtcagtttct ttatacgcat ctgccgaatc
    41341 tagttgattt gacaaataag tataatgaga tcaatgacca tgagatcaaa aacaaacaga
    41401 cctatgaaaa actagaagaa agcgcgcaga tcatcgacca gctgtcagct ttaatcgcac
    41461 aagattatca aaaattcgtt gctgaggatc tagatgatat cgacgtagag atatctgttg
    41521 cgaaacagag cttaaagcgt gataataaat atgaaccaga acaaaaggat taactctttt
    41581 gttcataaaa gaagactcgg aacattcaga tgatgtctgg ctgtttcgag tactttttct
    41641 aaagtagaga cattcatgta agaatcgaga acagattcaa caaggaggaa ttttttatgg
    41701 atcataaacc gactgaaaca aaagatgtta caccagttaa cgatacactt gaagatttat
    41761 tgaacaaccc gttttcaact ccaacagata cgctgaccac ttcacaacaa gcggagattg
    41821 atcagctcca agagcaacag acagctgctc gtttaattga caaactaccg gcggaacgcc
    41881 aagaacaagc taaacagcta gcagcaaaaa tcgatgctaa tgatgcacag tctgtcatca
    41941 gctacggatc ggctgcccaa gcaaaattaa gtgaattctc acaatcgatg ttgaatcatg
    42001 tgcaagcaca agatattggt cctgtaggag attccttaac tgaactgatg tatcgtctcc
    42061 aagaagccaa tcctgacgaa ctgcgtgccg gagaagggaa catcttccag cgtgtcttcg
    42121 ggaaggtcaa acaatcgatc tatgaagtga cagcaaaata tcaaaagatt ggtgcacaaa
    42181 ttgacaagat ttcagtgaaa ttagataaag aaaaagacgg tctgctaaaa gataatctga
    42241 tgttagaaca gctttaccag aaaaacaaag actactttga tgcactgaat atttatatcg
    42301 ctgctggaga attgaaaatg gaagaacttc aaacaacgat cattccagaa gcgatgaaaa
    42361 gagcagaaga aactggcgat cagatggatg ttcagattgc caatgattac acgcaattcc
    42421 tagatcgtct ggataaacgt acgcatgatc ttcgcttagc tagacagatc acgatccagc
    42481 aagcaccaca aatccgtttg atccaaaaca caaatcaggc cttggctgaa aagatccaag
    42541 catctatcgc tacagccatt ccgctgtgga aaaaccaagt cgtgatcgca ttgaccttgc
    42601 ttcgtcaaaa agatgcagta actgctcaac gccaagtctc tgaaacaaca aacgacttat
    42661 tgaagaaaaa ttcagaaatg ctgaaaatct cagccatcga aacagcgaaa gaaaatgaac
    42721 gtggaatcgt ggatatcgag acccttcaaa ccactcaaaa tgatttgatc gaaacaatcc
    42781 aagagacatt gcgtatccaa aaagaaggaa aagaaaaacg tcgtcatgca gaagtagagc
    42841 ttggacacat ggaagaagat ctgaaacaaa aactgttgga tttaacccaa tagatcacct
    42901 ttcgcttgtt actaaaacaa acgcccaaca acagtttatt acaactgttg atgggcgttt
    42961 ttcttatctt tcttctaact tctccattgg agtctatttc tcattcatct tgctgtgtcc
    43021 atagccatag ccaaaataaa tgatcagtcc aataaccatt acgataccga acacgatcca
    43081 tgtcacagtt tgcagacgaa gcatcaatac aatacaagtc aaaatggaga cgattggcaa
    43141 tagcggaacg aatggcactt tgaattctcc agcttttggt ttgccaaaca tttttctcaa
    43201 cttcagtaag cctagactaa caagcatcaa ataggcgagc gccatgatat tcgttagttc
    43261 cgctaataca ttcaatggga aaaatccggc aaagaataca gctgccacac cagcggcata
    43321 agtcgctttt ttcggtgtac gatgcttttg atcgatttct gtcataaact taggaagtaa
    43381 gccatccttg ctgacagcaa acagtaaacg agcaagtgaa tacatcatcg agatcgttac
    43441 cgtcagtagc gttaaaattg cccctacagc gatcaccgcg cccactgttc catgtcctac
    43501 atacctcatt gcaaaagcca ctgggtcatt gactcctagt tcagtatacg gaacgattcc
    43561 tgtcaagatc agcgtcacga tgatgtaaag gacagtagca atcagtatcg aaccaatgat
    43621 tcctcttggc acatcttttt gcgggttttt gacttcctcg gctgccattg caacggcatc
    43681 aaatcccaag aaagcaaaaa acacaagagc tgcaccatct agcacaccgg aaaaaccaaa
    43741 aggcatgaat ggctgccagt tatcaggttt aacgaaaaat attcctacaa tgataaacaa
    43801 cgcgatgata ccaaatttga catataccat ggcattattc aaacgtaatg ctttcttggc
    43861 ttcaagcgac acgatgtaag tgacgaatat cactacgagc accgcaatca agtcaatgta
    43921 tgtaccattt tccggattgt acccgccagt taatgcttgt ggaagttcgg cccctaaact
    43981 tttcaaaaaa ccttgcatat agccagacca tccggaagca acagaggaaa ccgctagtaa
    44041 aaattccccg atcaacaacc accctgtcag ccaggcagca aattcaccaa atacaacata
    44101 cagataggca tatggcccac caatcactgg cactcgcgaa gcaaattctg cgaaacttag
    44161 tccagacaaa atgacaacga ttgcagctaa gacaaatgaa agtgacagtg ctggaccagc
    44221 attttgattg gccgcaaccc ctgtcaccac aaatattccc gtaccgatga tcgcaccaat
    44281 tccaagcatg atcaaatcag acgtttttag ttcttttttc atctcactcg tatgattgag
    44341 attgatctct ttcttacgaa acaaatccat tgaagttcct ccccatttta ggttggaaaa
    44401 cagcgattaa actgttttca tataccctaa aaggatacca cctaaaatga ctaaaaggca
    44461 accaacaata acataaatga gctctttttt cgtttttctt tcgccaagga tgaaaatccc
    44521 acctaaagta gagataatga tccccatttg tgaaagtgag aagccaacag ctaaaccaag
    44581 tgatttgact gttagaagca tgcagatgtt tcctaagccc cataataatc cacagatcat
    44641 gtttttccac acaaactgat cgactttaac tttacggaaa gcaaagaagc ttgcgcctaa
    44701 gatcattccg atactttgag gtaagataat tgacatcgca tccaaattcg cccagttgac
    44761 gatgatggta tatacaccat aaccaatcgt agagaagatc aatgctctaa aacccttacc
    44821 aaaatcaagc attttattat ctgtatctat ttttccttcg tcatcttgtc gggcagtcaa
    44881 atacgcaccg cttacaagaa gagctaaagc aataataccg tatacataat ctttcgtttg
    44941 tgtccattca tggaagaaaa cggctcccgc aatcgtattc aaaattaatt gaaaaccagt
    45001 agacaatggt agccctacag aaacacccat gtacttcatt ccatggaact gtccattttg
    45061 tccaacactc caagctaagc cggataaaaa tccgatcaag aaaatccaag gggtgattac
    45121 aggactaaca acgaaaaaga caactaaaga aaacagaaat gcaccgatcg tcattcctag
    45181 tgtttgttga ttggcatcgc caccaatttt accacttact aaaccaatac taccccatgc
    45241 caacataggg actaatgcta ttaaaatttc cataaccaat ctccttttat ttcacatgat
    45301 aagaaactta tcagcgaaaa cgttaacaac acaagcactt tttttcgttt ctaacgattt
    45361 tctaacgttt ttcattataa aaatttaaga ttgggaaaaa taaagtcatt gttgtccctt
    45421 ttccttcagt tgattcagcc gtcaaatgga tacctaattt ttcggccaga ttttttgcta
    45481 aataaagtcc taatcctgtt gaatgtgttt tgctaagtcg accatttctc ccagtaaatc
    45541 ccttatcaaa gatccgtttc aagtcttccg ctgggatacc gatccctgaa tctttaactg
    45601 aaaggctgac tccttgcgct tccttttcta tgctcacaat gatgtttccg tgatccggcg
    45661 tatatttgat ggcattacta agcaattggt tgaagataaa agcgatccat ttagcatcgg
    45721 tcaaaacagt atgatcctca ccaacgatct cgtagcgaat atttttttgg ataaaataat
    45781 ttgcctgact gcgtatcaca ggctgaatga ttccttttag tccatattct tggatgaggt
    45841 agtcccgcga gaaactgtcg agtcttgcat aatacaaaac ttgttctacg tattcatcga
    45901 tttttgataa ttcattttct agtaatgtat acttttgatc gtcaatatcg aactcgatcg
    45961 aacgaactag caatttagaa gcggaaagtg gcactttgat ttcatggacc catgagtcaa
    46021 tataatcttt ttggtcttgt tgattactga ttgctgattg cattacttct tggtgttcac
    46081 ggatcagctg attgatatat tcttctacca gtttttcttc ttctgtcctc ccaccactca
    46141 aataattttg caaaggggaa gttttttgta tgtcggctaa ttttccccac catcttctct
    46201 tgctcaagta accacccagc agataaaaaa tcagaaagac gccctcaatc aaagctaaat
    46261 aagcaattgt gctcaaatca actttgatgt ttggcgccag ccacatcaca aagatcgtca
    46321 atgcaataaa aaacagccat cccaagatca atgtccactg atctttcaaa tatttaaaaa
    46381 aattcatctt aaccgctcat cctttcaggg aacgatatat ccctgtccta cttttgtttc
    46441 gatatatcct tggatacctg cctgttcgat ttttcggcgt aaccgattga tgttgactgt
    46501 caatgtattg tcatctacaa atcgttcgtc atcccacagt gctcgcagca atttttcccg
    46561 acttaagatc tttccattgt gcttcatcaa aataaataat agacgatatt catttttgct
    46621 caaatcaatg atctctccgt tgatttccat acttccgttg tcaacattca acgtaatgcc
    46681 attgtggctc atcatttcac tagataattc tgtgtagttg taagaacggc gaagtaatgc
    46741 attgattttt gcaatcagta catcgatttg gaaatgtttt gtcacaaaat catctgcccc
    46801 catgttcata gccatgatca tatccatatt cgtattcctg ctggagataa aaatgatcgg
    46861 tactttcgat acttcacgta ttttttgaca ccaataatag ccatccaaaa cgggaagatt
    46921 aatatctagt aatacaagct gtggttcttc tctttcaaag tctgagaaca cttgattgaa
    46981 atcggtcgtt ccaaaggttt caaattgcca tttctcaagt tcctccatga tcaattgtcg
    47041 aatgatctct tcgtcttcga ctaccatgat ttttgccata aaaaatccct ccatctttct
    47101 tcttgctact acaatatcaa agaagccctt gtgaaacaag ttggcttacc tggaaaatgt
    47161 cattttagca aacgttaaaa aaaactggaa atatgcgatt taatcgcgtt tgtctcttcc
    47221 aagcagctta aatttattat gttattctta ataattggtc tattatctgt tatttttctt
    47281 tcgttgtttt tgtcttttaa attttcgata acaccaataa ccgaaaagcg ttagcactag
    47341 caatatacct agaaggagta taagtaatcg ataaagatgg tatttttttg tctgttctat
    47401 ctttgaagag acttcttctg taaagggaac tcgtcttcct gtcactagta atcggtgagt
    47461 gttcacggtg taaggtgtac aagtgataag tgtgactaga tcctgctttt ctctaattgc
    47521 taatgtattt gtttttgttg gaaggacaac aattttctca acgacttcat aagcgagcat
    47581 ccgaccatac acttcaagat agaatcggtc atcttgctcc atttcttcaa gatccgtaaa
    47641 gagttttttc tcagcaatac cgctatgggc ggtaatcacg gaatgtgtac tctctcctcc
    47701 agttggaaaa gaggtgcctt gcaggactgt cgctcctcta tctaacaaaa gatcattcgt
    47761 ttcatcaaat aaaggcaagc taacattgat tttcggaata tagatggctc cgatcatatg
    47821 ttgttcgtaa tattcttttc ctggattttg gacatttcta accgcttgct caaaaggatc
    47881 tttgacttgt cccattcctg ggattactga tgtatgagca agcgtctgat ttttcttttc
    47941 atgcactgct aattgttgct catgtttttc ttctttatcg cgttcaagct gcttttgata
    48001 attctcgatc cttctttgat ccaaataatt gctgagagca tctgctaaaa atggatatgt
    48061 aaaaactgca gctccggcaa gaaacatcac tgccataaat aattgtaata aaagactgat
    48121 ttgggtttga ggtctattac ctattttctt tttcattttc ttcaccttct tatttaaaaa
    48181 aactgcagca acctttataa aaccgattgc tgcagcttat ttttgttgct tatacttcag
    48241 acttcatttt agatttacgg taccagctgt acgctccaat catcaaactt attccgatta
    48301 gtaagaatgc caagagcccg ttcccaccag tagcaggaag tgacccgcgt ttcatattcg
    48361 gtactctttc tggccctgga ttttctactc ctacgatctg aatttcttca ttatagcttc
    48421 cataagagat gataaattca gtaaaggctg cctcacctgt tggaagtaca taaccttctg
    48481 gcgccatggt ctctttcatt tgataggttc cataatctaa cccttgaacc tcaaagtatc
    48541 catttccatc tgcgacaaat tctgttgctt gtccgcttgt tgcccactca attctatctg
    48601 ctcctgattc ataaacagct aaaggcgact cccctttata aaatattgcg tattcagaac
    48661 ggtcttcatt ccacaattcg aatcttgcat ctttaagcaa ttcactgctt gatgcatcat
    48721 atttttcaaa ctttcttcca cctgttgtga ctttctctgg cggctctact ccttcttcat
    48781 aatcaaattc atcatttcgt ccgatttcaa taaccgctgt gttgttatgg aaatcatccg
    48841 cttctagttc tgtggaaacc ttcaaattat acgtgatcgt taaatctcct ccacgtgcat
    48901 ctgctaatct acctaaagta tctactttgg ctactccatt ttcttctgtc aaaatcgtca
    48961 cgacaaaccc gttgcttact acttctacta tataatcttg tccttttgtt aaaatcgtct
    49021 cgccggctcg cacttctatt tcctgatcat aataagttag accaactgta ggcgtatctg
    49081 tgattttgta atttttgata acagcgcttc cgttttcaag gacagaatcg atgaaataag
    49141 gaataggtgc tgtgatcgtg taagaagccg tttttcctct ttctagatca atataagaaa
    49201 tcgtttcttg ttctccagct tcattaataa tcgttacttg tctaaggtcg ctttcctcta
    49261 cattcatctg tttatctgct tctctgattt cgtttttcgg atacaaatgc acatctgtat
    49321 tgtatgtgtc tttccatgta gaaccatccc acacgccatc caccatatca ggcatcatca
    49381 ctggcatcat aacaaccatt ggagacgcga ttctggaaat atgagctggc gaataagttt
    49441 ctaagaaaag atagacttta tctctagaag aaggtgcttc ttccgtcact tggacggtag
    49501 agaataccgc ttctccagct tcattcgttg tttgtttgtc aatacttgtc agtcgatatt
    49561 ttaaaaacca actttctgtg tgccgttttt gaatccattc aatcgcttcg gcagaattaa
    49621 gaccgtcttc tcgttccggt gttaaaggat tatctcgaat caaatcgtaa tatacttccg
    49681 ttacgtcaaa caaatcaaaa tcgactcctg gaatattttc ttcaccaaaa tccgtagaaa
    49741 ccaatccgct attcggtcgt tcttccggaa cagatgagaa accttttttg tgtaatgtaa
    49801 tcgtcacttc acttggtttt tgtgcgactt cttcggcttt gactagtttt ccgttcccga
    49861 aaatccctat tcccgcagta acgagtaaca gtacactcat tcccgtgaca atcatttttt
    49921 tattcatttt tttctccccg ttcttttttt attaataaaa cagcacctaa caataaaaga
    49981 attcctagta aagaaatcaa cgttcttgct tcattcgttt gcgggaatac ggaccatttt
    50041 cctgattgac ctggattgct tggaccactc ggctctttcg gtttgtccgt tgtttctttt
    50101 acggtctcgt tataaacacg tggtgttgcg gattcatagg cagagactgg aactttcccg
    50161 gattctaatt cagccatctc ctgtccgtta actactacat attgtggttg gccttttgca
    50221 tccacccaaa attcagggat gatgacctcg attctcttac ttgcttcatc catctcgtaa
    50281 ccttccacac cttttaattc ttcaaaataa taggtgccag aaggcaagaa acgttgtcct
    50341 gtcgtaacaa ggccctcttt atccgaaatg aatttgctta catttttgtc attcaaagga
    50401 tcattgtctg ctggtttgat ccattgattt ttcaagtcat tagcaggagc catatctaaa
    50461 taatatttcc ccccttctat catctgatat agagcaaaaa cagcaccttc taatggtttt
    50521 cctttttcag ttgttcctct ttgacgtcca tacttgaaga agtatggatc tcttagataa
    50581 ccgacatttt taggatagat atgaatctct tgcagttcct cggttgattc tgtcgggtga
    50641 taaattggca aaatagcagc aattgggata gctgtctgat cgatatcaac atccaatcca
    50701 acttctggat caatcgattc ttcgaaaatc aaataaactg catcccgatc ttggtttttc
    50761 ttaggtgctt tgatacgtgc aatcccgtct cctaatccgc caaccgtatc tttcccagtt
    50821 gttaccgaag tccctatttg gacaagcctt tcttctgtga ttagctgacg gatattgttt
    50881 ctgttcgttt cagcgatttg ctggacgaat tctttaggag tcatgttttc agcagctttt
    50941 ttttcatgat aataatcggt catgtcgtaa atggcaaatg tcgctccatt tagtggcacc
    51001 gtctgtgaaa tgatatctgc tgtttcccct gcttcctcag agatagccaa gccatcattt
    51061 tcatggtatt caaattgatc gttataatcg atatctcgaa tcatccgttt atgcaacacg
    51121 aattccacga catcttcccc tgattcttct gccgacatcg catgtggaag aaggactgct
    51181 gcaagcggta gtaaaacaag taattgaagc atcaaacaca acagcttttt cattaccttg
    51241 ctccctcctt tttagattgt ctgacaaaca aaccataggc tgctcctgca agcgccatga
    51301 agaatgcagc aagcaggata tagaataatc gtccggatcc tcctgtagca ggcaacggat
    51361 tttcaggatc gttcccgaca ctaagctgta atctgattcg atttgtctcc aaaagttcat
    51421 attctaactc ttgctgtaga tcaggcgtct ctgcttcttc gtttggataa tacgaaatct
    51481 caacttttag tgtacccgct tcttttcctg gaactacccg atcattttct atgtcaggtt
    51541 cttctcgata actaatttct aaaataaagt aacctggaag ttttctatag ctgtcaggtg
    51601 acttcacttc cttgatccga tagacccctt ccaacaagtc tgaagcattg aatagggaag
    51661 aatcgtttgg atctggtgcc aagcctccct cgatgatttc atactctcct gcactatttt
    51721 gtttctcaat tgaaaattct gcgccttcaa gacgtcggct attcatactg tcaattttca
    51781 aaagttccaa atcgattggc gctagttcat cttctaaggt tacttgtagt acaccatcga
    51841 tgatttcata gccttccggc aagggagcag tttcgtcttc gatgaccgta tcttcaatga
    51901 taaacttacc ttcttcattc agtgcaaact ggaacacttg atcagatagc cgatacccat
    51961 ttggtgcgac agattccgag acctcatatt caccaataga cagatgcaga ggttggtcgt
    52021 aatttaaatc gacatacttt tctactccta cggcacgaga atagaagtat ccgatcccag
    52081 gcaaacgttt cgattcttct ggttctaacg gattaccagt gtatgctcga tacagcggga
    52141 aggaagaatc ccccactttc ctgatatcga aacctgcacc atctaatgta tggccttcat
    52201 ggtctaattt tctaactcgg atctcccgat aatcaaacgt attgtaagca gtgaatgcta
    52261 cccataaacg atcctcttca tctgttgtat acgtgagata ttgccaattt tcttcacctg
    52321 tttgcttgag ttggatagtc cctatagcat tatcgtaaag tcgcaagatc catgtgtcat
    52381 tttcatcagg ttctttgaaa ccttctgggt tctggctctc aatgattttg tattctgcat
    52441 agttggtagt ttcttcacta tccttataaa caaatgaatc tggctctccg attggatgaa
    52501 taactttctc accatccacg attacatatt tcccgtcttc atctactgaa tagaacgtga
    52561 gtctcccttt ctcttcctct acttcgttca caccagaaag ccctttcgct actggatatc
    52621 cgatttccag tccgttacca tctgtttcat aaagttcaaa ctctgcgcca ttgattggca
    52681 tattactgcc ttcaattagt ttattgatat tcatgtggat tggcttgaat tcatttcgca
    52741 tttccagttt gacatacaac tcatcatact ctgtgccttc tatgttaggc tcttgtacaa
    52801 cagaaagctt gtgatattct ccttcataat aggaattcgg atctttacct tccggtccgc
    52861 cgactctcga accagatgta tattcatcta caagctcgta gtgacctaac atctcatctt
    52921 cgttggctgc atattttttg atttggaatt gatacgtctc atctactaac tgataacgtg
    52981 gtggtccagc gatctctctg atccaaaata tgtcgctgac tgctccagtt tcatctccta
    53041 aaatcggcat cttcgtttca aagatcgcta ttccgtccgt ggtagtttta cttgtttcga
    53101 ttggttcggc ttccccactc tctggatttt ggtcatataa tgcaaattcg actccagcca
    53161 acggctcgcc attttgaccc ttcttgctga cttggaaatt acggccttta tgttcgtaac
    53221 gaatggaaga agatgggaaa tacatccgtc cagtagtgat atcattctca taatcctcca
    53281 ggttctgaat ataggccata ttgttcactg tgcaaaaatt tccgtcttga gctcccgcat
    53341 caagttttac ttggtacttg atcctgacca tttccccttc acctaaagaa atcggagaaa
    53401 atttataagc ttggttttcc cgtccgacat acgttgtcgg agtcgccact acttctggtg
    53461 gggcttgtcc atgacgttct tcatcccata tttctggctc ttggtacttc atgccgtttt
    53521 ctcctggatc tagatggtaa aactgaatat ctgccgatct gccagctcca aatggtgaga
    53581 cattaaaacc agtttcatca taaagtgtgg cttgctggat cgttctcttg aaggtagaag
    53641 agatatcctc caaggctgta acaatgtcat tcttgttgtt cgcattgtaa tatcgcggtg
    53701 ttacgccatc ttcttctaaa tcagcaatat ttttcaatac atttcgtcca atagcatcta
    53761 gtcgttgtct cccgctaacg tttccatcga ttcctaaacc aatggagaag atacttggcg
    53821 ggttctcttg atacttgttt ttcagccaat gatgataagc aactgtgttg atcgcagatg
    53881 acttttcttg cgaagtagga ctgccattcg tattgttatt agaagaacca tcaccaaccc
    53941 agtgagtcgc tttattatct ccttctttta cctctccata acgatagtgt acatttactt
    54001 catcttctgg acggttaaag tcagatgcat aggtaactgg atgagtaaac cctgcattag
    54061 aagtattccc agaaaactcc tcaaaatttc tgaacaaaac gtcattatct tcagcaatct
    54121 tatcttccat cacagtccag ttgctccaag ctccattagt gctcgtacga taacttgact
    54181 gaatcggtgc ataactgaag gtcggtgtcc catctcccac aacgattagg attttttctg
    54241 gatttctatt ttctccgcca ttgtctttat acaatgtttc atagccattt ttaagcccta
    54301 gtgtaagcgg agttcctccg attggtgtac gatttaatgc tgtattctca cttcccgtca
    54361 aacgattgat atcatttgtc aatggaaaga tgtccgaact gatttgctca tggttattga
    54421 tagctgtact attatggtaa aaattcacca taccgattcg aatatccaga ctcgggtcag
    54481 agagcagatc tttactcatt tcgtttactg cctcaatcaa cgcagcattt ttagtttgac
    54541 tattggtccc agcggttaat tcactcatac tcgctgactt atctagaaca aaaacgatat
    54601 cgattggact gatttccttt ttctcgcccc cgatgacatc tagatagaca ttgtaaaggg
    54661 ttgttgcatc ttcaggatgt agatcagatg ctgtgctgct tggcagaaca ggttctaccc
    54721 attttttgat gtacgccccg ttataatcca agtaactgtt ttcaaaattc cgttcatttc
    54781 cccaaagctg aacggtttga gctggaacgt ttctatcttc ttcgcctgca gttgatccat
    54841 tattagtagc atagtcgtaa tttcgccagt tcgctccccc gtttgtataa tcactatcat
    54901 cgatcaagtt ctctccgtca tatggcgagc ctttgtctcc ttgatagcgt tcattgcttt
    54961 gtgtccaatt tcctaacact tttgtatagt cattcgttgt aaacttcggg tagcgatgct
    55021 gttcgttttc aacttctccg ctaggataga tatattcata ggggtcttgc gtagctgttg
    55081 gcccaagacg ctccaaaata ccgttgaata actggcgttg aatgcgctgg agtgcttgac
    55141 cgctgggtac gattggttcg tccagctttt ccatctcatt ttcttcgcta cgttgaattt
    55201 cagcggaatc ttctgtatca gtttcatttt cttgtaattc ttggttatct attctttcat
    55261 caaccaccac ttgaagaggt agttaccctt cgttcacatc ggggatcttt tctgtttcaa
    55321 aaatcaatgt gccggtttct gcttcttttg aataaacttt tccgacatac cattcttgac
    55381 catcgatcgt ctcgactttt aggtcttctc catcttcatc ataggaggat agacctgttc
    55441 cacgaacgtt ttttacggta ccaatcccgg cggctgccgc atcgattgct aaacgtattc
    55501 taccttcatt ggcactttcc tttttttcaa aagccagatg ccattcaagg gtttcttctt
    55561 tttcaatata cttcccttca atttttgcat agtcctcatc taagaatacc tcatcctcct
    55621 ttgtttctgt tgactcagca gctacatcca gggtcgtcat atgatagact gactggaaat
    55681 atggtccaaa tagcaaactg acagttacta actgcaaaag cttcaatttt aaccctttca
    55741 catccttata ctcctttcaa aaatatttgt cttctttcac aacaatcaac aaaacaatgc
    55801 ccatctaatt ataatttaat ttaattttaa ttttaaatca aatgatatcc tttttagtta
    55861 aaaaacacca attatacaac atttattatt aaaattaaat tataaaattt tataacgttc
    55921 tcatttaaca aaacttaagt tattaagaca aatgaataaa caactacgct ttcctcgaaa
    55981 caaaaaaata aagcacaaat gcgcatttac gcactcatgc tttattcgta caattaattc
    56041 agataacggc agcgcatagg atgccaataa cacttcctaa aatagctcct cctaaaatgt
    56101 ca
//
LOCUS       DANKKG010000004        55538 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000004 DANKKG010000000
VERSION     DANKKG010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55538
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.004"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            12..266
                     /locus_tag="PW004_000172"
     CDS             12..266
                     /locus_tag="PW004_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HDL2725173.1"
                     /translation="MLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQI
                     AKENGGTFHEVDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            545..1045
                     /locus_tag="PW004_000173"
     CDS             545..1045
                     /locus_tag="PW004_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HDL2725174.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(1128..1679)
                     /gene="lepB"
                     /locus_tag="PW004_000174"
     CDS             complement(1128..1679)
                     /gene="lepB"
                     /locus_tag="PW004_000174"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDL2725175.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(1831..2058)
                     /locus_tag="PW004_000175"
     CDS             complement(1831..2058)
                     /locus_tag="PW004_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HDL2725176.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(2055..2573)
                     /gene="msrA"
                     /locus_tag="PW004_000176"
     CDS             complement(2055..2573)
                     /gene="msrA"
                     /locus_tag="PW004_000176"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDL2725177.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(2593..3306)
                     /locus_tag="PW004_000177"
     CDS             complement(2593..3306)
                     /locus_tag="PW004_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HDL2725178.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(3309..4205)
                     /locus_tag="PW004_000178"
     CDS             complement(3309..4205)
                     /locus_tag="PW004_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDL2725179.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(4363..5205)
                     /locus_tag="PW004_000179"
     CDS             complement(4363..5205)
                     /locus_tag="PW004_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDL2725180.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            5337..5990
                     /locus_tag="PW004_000180"
     CDS             5337..5990
                     /locus_tag="PW004_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HDL2725181.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(6053..6580)
                     /locus_tag="PW004_000181"
     CDS             complement(6053..6580)
                     /locus_tag="PW004_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDL2725182.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6600..7547)
                     /locus_tag="PW004_000182"
     CDS             complement(6600..7547)
                     /locus_tag="PW004_000182"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDL2725183.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(7559..9445)
                     /locus_tag="PW004_000183"
     CDS             complement(7559..9445)
                     /locus_tag="PW004_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDL2725184.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            complement(9605..11683)
                     /locus_tag="PW004_000184"
     CDS             complement(9605..11683)
                     /locus_tag="PW004_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HDL2725185.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(11696..11971)
                     /locus_tag="PW004_000185"
     CDS             complement(11696..11971)
                     /locus_tag="PW004_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725186.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(12333..12788)
                     /locus_tag="PW004_000186"
     CDS             complement(12333..12788)
                     /locus_tag="PW004_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725187.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(12942..14150)
                     /locus_tag="PW004_000187"
     CDS             complement(12942..14150)
                     /locus_tag="PW004_000187"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HDL2725188.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(14262..14594)
                     /locus_tag="PW004_000188"
     CDS             complement(14262..14594)
                     /locus_tag="PW004_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HDL2725189.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            14742..15617
                     /locus_tag="PW004_000189"
     CDS             14742..15617
                     /locus_tag="PW004_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL2725190.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(15699..16280)
                     /locus_tag="PW004_000190"
     CDS             complement(15699..16280)
                     /locus_tag="PW004_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HDL2725191.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(16286..17545)
                     /locus_tag="PW004_000191"
     CDS             complement(16286..17545)
                     /locus_tag="PW004_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDL2725192.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(17547..18593)
                     /locus_tag="PW004_000192"
     CDS             complement(17547..18593)
                     /locus_tag="PW004_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725193.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(18828..19103)
                     /locus_tag="PW004_000193"
     CDS             complement(18828..19103)
                     /locus_tag="PW004_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HDL2725194.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(19346..20656)
                     /gene="der"
                     /locus_tag="PW004_000194"
     CDS             complement(19346..20656)
                     /gene="der"
                     /locus_tag="PW004_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HDL2725195.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(20838..22067)
                     /gene="rpsA"
                     /locus_tag="PW004_000195"
     CDS             complement(20838..22067)
                     /gene="rpsA"
                     /locus_tag="PW004_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HDL2725196.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(22202..22882)
                     /locus_tag="PW004_000196"
     CDS             complement(22202..22882)
                     /locus_tag="PW004_000196"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HDL2725197.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(22931..23566)
                     /locus_tag="PW004_000197"
     CDS             complement(22931..23566)
                     /locus_tag="PW004_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL2725198.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(23632..25035)
                     /locus_tag="PW004_000198"
     CDS             complement(23632..25035)
                     /locus_tag="PW004_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HDL2725199.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKNMEH"
     gene            complement(25022..26053)
                     /locus_tag="PW004_000199"
     CDS             complement(25022..26053)
                     /locus_tag="PW004_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2725200.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            26084..26305
                     /locus_tag="PW004_000200"
     CDS             26084..26305
                     /locus_tag="PW004_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HDL2725201.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(26470..27207)
                     /locus_tag="PW004_000201"
     CDS             complement(26470..27207)
                     /locus_tag="PW004_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HDL2725202.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(27360..28700)
                     /locus_tag="PW004_000202"
     CDS             complement(27360..28700)
                     /locus_tag="PW004_000202"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HDL2725203.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(28937..30460)
                     /locus_tag="PW004_000203"
     CDS             complement(28937..30460)
                     /locus_tag="PW004_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HDL2725204.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(30838..31449)
                     /locus_tag="PW004_000204"
     CDS             complement(30838..31449)
                     /locus_tag="PW004_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL2725205.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(31572..31697)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(31779..32495)
                     /locus_tag="PW004_000205"
     CDS             complement(31779..32495)
                     /locus_tag="PW004_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDL2725206.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(32501..33070)
                     /gene="scpB"
                     /locus_tag="PW004_000206"
     CDS             complement(32501..33070)
                     /gene="scpB"
                     /locus_tag="PW004_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HDL2725207.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(33054..33851)
                     /locus_tag="PW004_000207"
     CDS             complement(33054..33851)
                     /locus_tag="PW004_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HDL2725208.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(33821..34192)
                     /locus_tag="PW004_000208"
     CDS             complement(33821..34192)
                     /locus_tag="PW004_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDL2725209.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(34211..35098)
                     /gene="xerD"
                     /locus_tag="PW004_000209"
     CDS             complement(34211..35098)
                     /gene="xerD"
                     /locus_tag="PW004_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HDL2725210.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(35194..35667)
                     /locus_tag="PW004_000210"
     CDS             complement(35194..35667)
                     /locus_tag="PW004_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDL2725211.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(35772..36635)
                     /locus_tag="PW004_000211"
     CDS             complement(35772..36635)
                     /locus_tag="PW004_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDL2725212.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(36831..38930)
                     /locus_tag="PW004_000212"
     CDS             complement(36831..38930)
                     /locus_tag="PW004_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HDL2725213.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(39150..40259)
                     /gene="rpoD"
                     /locus_tag="PW004_000213"
     CDS             complement(39150..40259)
                     /gene="rpoD"
                     /locus_tag="PW004_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HDL2725214.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(40283..42124)
                     /locus_tag="PW004_000214"
     CDS             complement(40283..42124)
                     /locus_tag="PW004_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDL2725215.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(42306..44621)
                     /locus_tag="PW004_000215"
     CDS             complement(42306..44621)
                     /locus_tag="PW004_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDL2725216.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(44806..45666)
                     /locus_tag="PW004_000216"
     CDS             complement(44806..45666)
                     /locus_tag="PW004_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HDL2725217.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(45725..46246)
                     /locus_tag="PW004_000217"
     CDS             complement(45725..46246)
                     /locus_tag="PW004_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HDL2725218.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            46524..47285
                     /locus_tag="PW004_000218"
     CDS             46524..47285
                     /locus_tag="PW004_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL2725219.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            47311..49902
                     /gene="mprF"
                     /locus_tag="PW004_000219"
     CDS             47311..49902
                     /gene="mprF"
                     /locus_tag="PW004_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HDL2725220.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(50090..51277)
                     /gene="tuf"
                     /locus_tag="PW004_000220"
     CDS             complement(50090..51277)
                     /gene="tuf"
                     /locus_tag="PW004_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDL2725221.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            51645..52001
                     /locus_tag="PW004_000221"
     CDS             51645..52001
                     /locus_tag="PW004_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDL2725222.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(52242..53246)
                     /locus_tag="PW004_000222"
     CDS             complement(52242..53246)
                     /locus_tag="PW004_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HDL2725223.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            53412..54263
                     /locus_tag="PW004_000223"
     CDS             53412..54263
                     /locus_tag="PW004_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL2725224.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            54280..54525
                     /locus_tag="PW004_000224"
     CDS             54280..54525
                     /locus_tag="PW004_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725225.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            54712..55416
                     /locus_tag="PW004_000225"
     CDS             54712..55416
                     /locus_tag="PW004_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725226.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT        15620 a        11547 c         9537 g        18834 t
ORIGIN      
        1 ctttttggat aatgcttctc tctcatgatt tttataaaaa atttataaaa gataaagaaa
       61 tagctgataa taaaaaaaca agtggtgctt tgatttcgtt tagtgttgaa aatgcggatg
      121 aagtaaaaaa atttgctcaa attgcaaaag aaaatggtgg tactttccac gaagtagata
      181 tgggaatgcc tgaagaagaa atgttttcat tagaagtcca agatctagac ggaaatacat
      241 tagaacccgt atggatgaag atgtaagcag ataactaatc tgacgttttt tataacaaaa
      301 aaacaaaggc ggatgatacg ataagtattc tccgcctcta ttttttttat tcgtacaatc
      361 tcttttttac agtttataaa agcagtcttc tcaccctttt tttaattgtt agatgccagt
      421 gtatgtaaat caccaatgtg tcatgacaca ttggtgattt tatggtatcc ttttattatt
      481 aactgacaaa ggagagaata ttttgaacac tttgattgca aaagacgttt ttagattgcc
      541 cgaaatgaat gcaagattgt tgtcgacctc tgctctttta ttagcccttg atgtcatctt
      601 atacaagata gcgattggtc ctgcttattt tcaaatcaca ttcggatttt taagtatggc
      661 gcttatgggt tatctatatg gaccgatctg ggctggtttg cttgaaatgt tgtcgaacac
      721 tttaaatttc acgatatttg gttcaggaac gtttcaagct gcttttctta tcacggcttt
      781 ccttggtgga tttattaatg gcttattttt atatcataaa aagataaacc tgccttccgt
      841 catcgctgcc caattattga ttatgatccc agtcagcctt tttatcaatt cttggctgat
      901 gtctgttttg tttggtgcag atttcaaagt aatttttcta ggtagattca tcaggaatat
      961 gatccaaatc cctattcaaa taatggttgt ttattcattt atcaaaactt tagaggctag
     1021 aaaagtgttt aaacgagctg tttgatttta cgtactaagg tagtaaactg attttggatt
     1081 aaacaataaa gcgtgcagtc tcaacagact gcacgcttta ttgtttctca ctggatccat
     1141 tttatttcat caagtggata atagacaaat cttgctttac ctagaatatc agttttattg
     1201 atcgtgccaa acgaacgact atcttttgaa actcttcgat tatctccaag tacaaaataa
     1261 ttatcttccg gaagcttgga ttgtcccgtt agctcttgta gtgtaaagtc cgtcgtatag
     1321 gatgcatttg catgatctga tttcaggttt ttagttagat aaggttcctt aactactttc
     1381 ccattgatct tcaattgatc attttggtag ctgacatttt ctcctggaag accgattact
     1441 cgtttgatat aaatcgtgcc atctgctaat tgaaaaacaa caatatcaaa tcgtctgatc
     1501 tccgaaaatt tttccatcac aaccatatca ccttgcttca acacgttttc cattgaattg
     1561 ccttccactg gtaccgggat gaataaaaat ccccgcaaca tgaacgcaag gaatacagaa
     1621 acaagtaagt atttgaatac aagccagaag cgattaatat agcgttgttt ttttgtcaat
     1681 ctctatcaat ccatccctct ttttttcatt ctttctttat tataagaaaa atatcaaaaa
     1741 aatgcgacta gatagctgaa ataaaaataa ttctttcaat taggctattg gacaaaaaac
     1801 ttttttgatc ataaagtaca aaactatctc ttatatgtca tgattatttt ctaaatattt
     1861 ttcccatgct tcgtcaaaga tatccatgga cggcaaatag tcgacattca gttccagata
     1921 attagaaatt tcttcgtagc tagagctgtg cttaggaaat tggatgtctt taccggcttc
     1981 tgtagcgaaa acagataaag catcatgtgt gccaccgcct cgcagtgtca ttaaataatg
     2041 aaaaaagctt cgattcaccc ttcgttcctc caattttctt cgataaaggc agcccggcgt
     2101 gcatggtttc ttttaaaatt ttcaggatgc tttttataat actcttgatg gtcttcttct
     2161 gctagataaa atggttgggc tggttcgatt tttgtaacga ttggctgttc gaaacgtcca
     2221 ctttcagcta actgtttctt acttttttca gcaatgatcc gctgttcttc tgtcaaataa
     2281 aagatcaccg ggcgataatt gtcaccgcga tcctcgaatt gtccgaaagc gtcagttgga
     2341 tcagtttgct gccagtaaag ttctactaat tgttcatatg aaatcaatgt tggatcatag
     2401 ataatctcca ctgcttctgt atgtccagtc gtttggcttt ttacttgttc ataagtcgga
     2461 tctggtacat gccctccagt atatcctgac atcactgtat ggataccagg aagcgtgtcg
     2521 aaaggctgga tcatacacca gaagcagcct cctgcaaata ttgctcgttc cattatgatt
     2581 ccccctctaa tactactcat ccgatgattt tttattttct tttgtcttgc tttcagtact
     2641 gctggtgcta gttttgttgg tatttgagtt gtttgaagca tcagaggtat cttttggcaa
     2701 atatagacta aaccgaatgt catcatttac taagtcgatt ttatctgctt taatatacat
     2761 cccgttttgc attcggaatt gatccaacct aagcatgata gtcagatcat ctgtattgac
     2821 ttcgatccaa tctggtaatt tatagtttct cttgatcata ttcatcacat ctttgattgg
     2881 caaactcaat gttccgatag aaagacttct agcttttagt tggacatttc cgttttccat
     2941 cacatacgga tcaaaatata aatagaaatc gataggaaaa cctagtactt cgaaagtacc
     3001 attgagtagt gcttcatttt ccaaataaaa tttatattca atatcactat ctttttgata
     3061 ctcactaagg aaaaagctga tgatttgatt gactttttct ttatttgaat tgatcgttaa
     3121 gacaggatct ccttgtcttt ccgtaagagc tggtatttct gggagttctg gttcccgatt
     3181 agcaaaaata cggctgccta aaaatacggt cgaaccaagt actaccccaa gaaggataag
     3241 gaaggcgaat ttccagtaat ttgttttctt tgttttgttt gaagatacgt cagatcttgt
     3301 tttcatgttt attgatcatc ctccttctct aaccattggt tttgtgtctc gatcagctga
     3361 tctctcactg catttgccat caattgataa cctaaattat ttggatggaa atgatcttct
     3421 tcatagagca cattattttt gatgccattt agattttctg tcgtgctagt tgtttgatct
     3481 gttccttctt ctgtgacacc tacttcctga ttcaatcctt gataaagcaa atcattgatt
     3541 gggataaagt agcagttcgg catttttttt gtcaccgttt ctgttccttt gttccaattg
     3601 ttaacaattg tctgcatgtc agtgatctca ggaaagttca aataaaatgg attgtagatg
     3661 cctaaaatgt aaataggtgc ggttttattg agggatcgaa attcttccaa tagttctgtg
     3721 acattttcct gatagttttt cagtggcttt ttaaaggaat tgaccgttaa cccgaataaa
     3781 ttattttgga taactttcat caaatcatta ccgccaacag tcagtgtgat aaaatcagct
     3841 gttttgatat tgtttcgaat ttcttctttt gtttttactc gctttaagat ctggtcactt
     3901 cgttcaccat ttacaccata gttttctgtt tcaaccgcgg tcaaattata acggtctttt
     3961 agatcatttg cgacaagggg aacgaatcct ccttgcttgg tttgatctcc tactccttca
     4021 gtcagcgaat ctccgatagc tatgtagtga agtttttctt ttttatttgg atcttctctc
     4081 acagtagtta ctggtttcat tagtgggcca gctttcggta tagaagcagc taaacctgca
     4141 tagatgatga tcgcaattaa taatgggaac aaaacgagga tcaaatgtcg tttcgttaat
     4201 ttcatttttt cacctcttgt attcgattgg ttctaatgtt atcaaaaatt ttatcaaaaa
     4261 ggtagtcttt atgaaaaaag aaaacaatta ttttcctaat tttataaaga aaaagagaat
     4321 aagctgcaag cctttcaggc tgagggctta tcctcttttc gactaatcta cataatacat
     4381 gattgcaaac gcgtctttac cagtatgcgt agcgataacc ggattcgtat gcagtacagg
     4441 gatatgcatg tccgggaata attcttgcaa accctctttg aattcttgcg ccaattcttt
     4501 tgcatccgca tgtgaaatac cgatttgtcg cacatttggc aattctttca gttcattttt
     4561 caaattatta aaccatttat cgaacgtttt ctttcctcgc cccttagtta cagggattaa
     4621 ttcagaatgg gcgaaattca tcaccacgcg tatgttcaaa acactagaca gcaaaccagt
     4681 tgttcggctg attcttccac cttttaccaa gttatctagt gtggatattc cgatgtataa
     4741 ttttgtattg tcttgtactt ttttgacttc ttcaaggatt ttctctactg ttttaccttc
     4801 ttttgctaat tcagctgctc gaattacctg aaaagataat ccttggtcag tggtatcact
     4861 gtcgataacc gttactttcg tttttgttag attactggct tgtcttgctg cttctactgt
     4921 tccactcaag ccttttgtca tatggatcga aatcacttca ctgccgtctt ctcctagctc
     4981 atcatacatc gagatgaatt ctccaattgg tggttggctt gttttgggca gattttttgc
     5041 tttcgccatc atttccataa atctttcgcc gtctagttgg tcatcatcgg gatatactgt
     5101 tccgtcgatc atgatggata gcggcatgac acgaatgcct aaagcatcgc gtacattttt
     5161 ttccatcgta catgaagaat ctgttacgat ttttacattt gtcattattt tctctctctc
     5221 tttctttaac aagatgcctt attcatgtta aactacatgt aagacaattt ctatttttag
     5281 tataacagac aaataatgat ttttcattaa ttacacctag taaaggagcc gttttcatgg
     5341 aaaaaacgaa atttaatcga acgtatgaaa tattaaatga agtgtttaat gcagtcaccc
     5401 atggaattgg gactggattg agtatcgcgg gactagtggt tcttttagta aaaggtgccc
     5461 acttgcattc ctccctccat attgtctctt atgctatcta tggatcgatg atgattcttt
     5521 tatttttaac gtctactttg tttcattcat tgatttttac aaaagcaaaa aaggtgttcc
     5581 aagtattcga tcatagttcg atttttcttt tgattgcagg aagttatact cctttttgtt
     5641 tgctgagtat ccgaggatgg ctaggttgga ctttatttgt attaatctgg ttgtttgcga
     5701 ttcttggcat tatatataaa gcgttgacgt tgcataaacg ggagagagta tcaaaagtat
     5761 ctacgatcat ctatattttt atggggtggc tctgtgtcgt ggctgctgtt cctctttatc
     5821 actctcttgg aatgactggt gttattttga tggtagcagg cgggctttct tacacgatag
     5881 gcgcattctt ttatagcttg aattctatcc gctatatgca tgttgtttgg catttgtttg
     5941 ttatgttagg tgcaggtttt atgtacttct ctgttttatt ctttacttaa taaaaaaaat
     6001 aaagctatgg atgggacagc tctaaataac agctagtcta tccatagctt ttttatatcg
     6061 tcaattcgcc tgtcccgctt tttacaggat tctcattacg ctgatacgtt tcaaattgat
     6121 gcggataacg atttttttca tccatttctc cttgactagt attgatcagt gaccaatcag
     6181 accagtcaac aggtggaaaa taagcatctc catcgaatac atgatggatg actgtacggt
     6241 ataatatcgt acaaaatggc agaaattctt gataaacagc tgaccctcca gcgatgaaaa
     6301 agataccgtc tgtttctttt tccagagcaa gcacgtcttc cacactatgc atgacatgca
     6361 cgccttctgc tttataggaa gaatctctcg tcaaaacaat cgtctgacga ttcggcaacg
     6421 gacggctacc catcccttcg aatgtttttc gtcccatcac tagtgtattt gcttctgtca
     6481 tttgtttgaa gaatttcaga tcatttggca aacgccaagg aagacggtct tcttttccaa
     6541 tcaatccgtt ttcatcctgt gcccaaattg cagctaacat tttctcatcc tttctgattc
     6601 taaacagcga taggagcttt gatcgccgga tgtgggtcat atccttctaa ttggatatct
     6661 tccatctcaa aatcaaatac tgactgcttt tcctgattca aaactaaagt cggaaatgcg
     6721 cgtatttctc ggctcaattg ttctttcatt tgttcgatat gattgatata aagatgagca
     6781 tctcctaatg tgtgaacaaa ttcgccaacc gctaaacctg tttcatgtgc aattaggtga
     6841 gtcagcaacg cataacttgc aatgttgaac ggaacgccta gaaacacatc cccactgcgt
     6901 tgatacaatt ggcagcttag ccgtccctct tgaacataaa attgaaacat cgtatgacaa
     6961 ggaggcaaag ccatgctagg aacatcttcc ggattccatg cagaaacgat caatcttcta
     7021 gaatcaggag tctttttgat tgcttcgatc acttgtttga tttgatcgat aaagccaccg
     7081 tcttttgttt cccagtgacg ccattgatag ccgtagatgt gtccaagatc gccgtatgtt
     7141 tcagcaaagg aatcatcggt taggatcttc tcgcaaaaaa gcttgctctg ctttttatat
     7201 tcttcattga attcaggatc tgtcaaacat ctatggccaa aatcagtcat atctggtcca
     7261 ttatagtctg gacttttgat gtagcgttca aatgcccatt catcccaaat atggttttta
     7321 ttttgtaata gatagcggat attcgtatct ccttttaaaa accaaagcaa ttcgcttttg
     7381 atcaatccaa aaggtactcg tttcgttgtt aacaaaggaa accctttttg cagatcaaac
     7441 cgcatttggt atccgaataa gcttaatgtg cctgtgcctg ttctgtcttc tttacgatgc
     7501 ccttcttcca acaattttct tcctaagtca agatacgctt gttccatcct ttttcctcct
     7561 aatcttctac ccattcactc aagtattccc accgtgccat tttttcttca agttgtgctt
     7621 ctgtttcaga tacgtcgttt tgcaattctt gtaatcgagt gaaattatct ccttgatgat
     7681 tcatctcttc ttggagcaat gagatttttt cttcaagttc tgcaatctca tcttcaatcg
     7741 tttcccattc tttttgttcc atgtaagata atttcttctt ttcagcaggt tccttgctct
     7801 gtatttttgg tttcgctttt tcagcaggct gctgggtctc tttttgcttc agtaaataac
     7861 tggacatgtt tccgtaataa agttcttgtt ttccgtttcc ttggaatact aataacttat
     7921 ctgctacctt gtccaagaaa taacggtcat gggaaacagt gatcacagca cctggaaaac
     7981 ttctaaaata atcttccaaa atcgtcaatg ttgctatgtc caaatcattg gtcggttcat
     8041 ccaataataa aacattaggt tgctgaatca gcagtttcaa caaaaataac cggcgttttt
     8101 ctccgcctga aagtttgcct atcagtgtcc catgcataaa tcttggaaat aaaaatcgtt
     8161 caagtaattc tgctactccg atttgactgc catctgctgt ttttgcttct tcagcagctt
     8221 cttgtaagta agcaatcatc cgtttatctg gtgccatttc ttcattttct tgtgtataat
     8281 acgcaagatg aaccgtttca ccgatagaca ttgtaccgct atccagggga attctgccag
     8341 ctagaatatt aagcaatgtc gattttcccg caccattttt accagtaata cctagtcttt
     8401 cccgagattg aatcaataaa tctaaatgtt ctagcagtgt ttggtcattt atacggtaac
     8461 tgccatcttt gatttccagt acttttttcc ctaaacgttg tgtcgctagg ttcagctcta
     8521 aatcatcttc ttggtttact tggtacaagt tctcctttag atcttcgaaa cggttgatcc
     8581 gtgcttgctg cttggtgcca cgtgcctgta cgccagccct catccaagct aattcctgtt
     8641 tataaagccg cttgcgcttt tcttcttgtt ctacttctac acgatcacgt tctgcttttt
     8701 ctaagacgta agcttcataa tttcctttat actcgtataa ttttccaaat gaaagttcga
     8761 agattcggtt tgctactcta tctagaaaat aacgatcgtg ggtaaccatc aatagtgcgc
     8821 cacggtattg tttcaaatag ttttccaacc actcgatcgc ttcataatct aaatggtttg
     8881 ttggttcgtc tagtaatagt aaatcgggtt catcaatcaa cacttgcgcc agactaactc
     8941 gttttttctg accacctgat aaagtactga tttttttatc taattcactg attcctaatt
     9001 tttgcaaaat aatctttgcg ttggtatctg tagtccaagc atcttcttta ttcatttgtt
     9061 cttctgcttt agcataacgt ttttgcacat cagggtcttc tccgttttcc cctagttcga
     9121 tcaatgcttc ctcatatgct cgaacagttt ggatcaaggg gctgtttcct tgaaagactg
     9181 cttggagaac agttaattca tcggaaaatt cagctgcttg agaaagataa ccaatacggt
     9241 aatcagttgg ataaaacact gtttgccgat caccgtcgct gctatcgata ccggcaataa
     9301 tatttaagag acttgtttta cccgtcccgt tcgtcccaat caacccgata cggtcttttt
     9361 cgtggaccaa aaaagaaatc tggtcaaaca aatctttttc accataagtt tttttgaggt
     9421 cagttacttt taattctttc aacatgttcc ctccttatct tggatattgt atcataaaaa
     9481 tagtgaactt caggaaaatt tttttggtat ttgttctttt tgtgttctca aaaaaagaac
     9541 aggaccgatt cgatcctgtt ctgatatgtt ttgtttttta taaaacttga aaatttttac
     9601 ctttttattt tattggactc gttttttttg actttgctcg aaacgcaaat cggaaagaaa
     9661 agttatcaga tggtttgttc atcgtataag attctaacaa attgatattc ctgcgatagc
     9721 tgttcgcaga aaacgtcgtg attttttcag caacttcgta ttcatcaatg atttttcttt
     9781 caagataata acctcttagc atttttttcg taaacagact atcttgctgg atcatccacg
     9841 aagaaatcaa attccccgag ttcattttta ttccttcaag tttgcgttgc tggataaaaa
     9901 attgaaccat cgtttcatca taaatatcag tcaagttatc taaactttca ataatcaact
     9961 gagtattttt ttgatatgtc tctctgattt cttggatatc ttcatcacta ataaatgtct
    10021 gacgatcagc atatcttctt tgccaaaaca ttttcggatg gagaacgatt cgcattaacc
    10081 tacggacaac aatgaaccaa aagccgataa aagacaaaat ctggcttgtg atagaatgtt
    10141 cgaatcgaga aagaaaacgg gagtaaaaac gataaccatt agaagaaagc ttgcccgaac
    10201 gataactttc atccaatcca tcccgttcga tcgacaaaat cagtgcttgt atttcttgta
    10261 cttcttgctt ttcatcatcg gttaaatctt ccaagtacag atcacgtatc cgttcttgat
    10321 aattttctat cactgcttca gtggctaaaa attcttttgt attacgatca atttccttta
    10381 tttcttttcg caaaacttca acgacctctt ctaaaatcat caaagcattc aaatccgtgc
    10441 ttttcgcttc ctctgtttcc gtcagcatag gcaagacaat gatccctatg accaaagtca
    10501 ctaagatgac acccgccgtc agaaacaaaa gcaaagaccg ctggtaaaac atcatattat
    10561 tgattgaaaa tggcaaaata aaaatcgtag ccaaactgac tgttcccttt acaccgccaa
    10621 atgttaagat caagatctcg ttcaactgtt ttttgaactt tttactgcca tctttgaata
    10681 cataaaaaag actgatagaa atgaatctga tcacaaaaag catgacagag attaatacga
    10741 taatcgctaa taaaagaccg tttggatata aaccgtctcc ccatactggc gagaacactt
    10801 gcgtcaattc gattcctaaa aatataaata ctaatgcatt cagtgtaaaa gcaatagttg
    10861 tccatgtact gtgggacaag ctcgacaatt ctgcatcaaa cacagagatt ttcctaaaac
    10921 cagaagcttg gagaatacct gctgccactg cagcaatgat tccagataca tcaaatgcct
    10981 ctgccaatac ataggctaga aaaggaagca aaagttcgat cagcagatac cctgtgacat
    11041 cttgtgctga tgctttttcg atcaaatgaa ttattttttg tttgaaccag acaagcaaaa
    11101 agccgatcaa agctccccca atactagaaa ctactagact catccccgcg tttactgctg
    11161 aaaaagatcc ggtgatcagc gcaccaagag caaactggaa ggcagtaaca ccagatgcat
    11221 cattcaaaag accttctcct tccaaaatat gcatcgcctt tttagggata ttcactcgtc
    11281 cagaaagaga actgactgct accgcatctg tcggacctaa cgctgctcca aaagcaaagc
    11341 aggcagcaag tgggacactt ggcaacagga atgataacgt cgcacctacc ccgacaaggg
    11401 tcaaaatgac tcctccaaat gccaagaaca aaattgtccc gaaatttttg agaatcgaag
    11461 aaatatctgc tttttctcct tctctaaata acaatggtgc aataatcata actaagaaca
    11521 attcaggttc aaaatcgatt gattgtcccc actctgtcag acctaatacc actccaagga
    11581 aaatttggat cagtggagct ggaatcattg gcaaaatgcg gctagctatg ttcgataagg
    11641 tgatcgttac tgcaaataca ataactaact cagcaaattc catcgtccat cccctttatt
    11701 ttttattttg tgtaagcatt tttaaaaatt gttctttttt ctttaaaatt ttggtttcga
    11761 ctacttcaac ctgtgtttgg ttcccctcta attcacactg tacacgtttt gactccaaac
    11821 gaagcagatc atgtttcaga cgttccaact gacgatacat caatatattt cttttacgca
    11881 acttacaaag tatttgacgg cggtcatcca tcgaaagggc gccatagcga tcatgcaaca
    11941 gttttttttg ttgttcaaaa ctctctctca ttttccagat ctcctataca caaacttcca
    12001 tcattatagc aaagttcttt ttggatgaaa aatatttcat tgtttcatcg tttattgcta
    12061 gttagaaatt tccataaatt tttcaacgcc tcattctatt ccaaatagta tcagatatca
    12121 agtaaaactt aagaaagata aaagtttctg acaaaaggat gtttactttc ccattcctgc
    12181 ctttcttgtt cttttatcac aacaaaaaaa tggggctgcg acaaaaatct tgtcacagtc
    12241 ccttattctg aataaacggt gtggaagaga cagcctatca gtagtaataa gacacatctc
    12301 tttcacagcc tcatttttgc gtaaaaatcg atttattcga aaaattcccc gtatacatct
    12361 tctacacgtt gatctggatt gatcacgata tacaagttac ctgttttttc gctgattttt
    12421 gttgattgat attgattgtc taatccttct gttcctttat ctagatatgg gaagtacaca
    12481 agatcaggtg tgccatttcg gtaaaggatt tccattcgct cgatatcttc gtatttcaat
    12541 gcgcgattaa acatgcccat ttccagtccg cctaggttga tatcatgtgt cgcaactgcg
    12601 tccccttcat ggaagatttc gattttaaat ccagcacacg gatggatttc aacaaattca
    12661 ctgccattga ttcgtccaaa acttgttgtg atttgtttga tccaaagatc gccgatatga
    12721 cggcggttga tcgtccaggt ttctccattt ctaaaataaa attttagcgc tgtcatttct
    12781 ttactcatta aaaagactcc tttatgatgt aatttattct tgtactacct cagtttagca
    12841 caaatttatg taagtggtca catcttatgt tttttcttct aatgacgtat ctgcccactt
    12901 taagtagcca acaaagctga gcagatacag tctagtgttt tttaaatcaa tttttctttc
    12961 gataaaaaat ccaaaacctg ttttttatca ttttttattc tttgttctac gacggctttt
    13021 tcagctaatt gcaaagcttc actaacccac ttaccaggtt tttttccagt ttcttctagc
    13081 aagattttac catctactgc taattcgctt aatgacttga taggtaaacg atcataggat
    13141 tctcctacgg catccatctg aggtgtttcg tcaaaataga tcatcaattc ttctacttgc
    13201 agtgcatgtt ttttgcctaa gaagtacaat tcgaatggtt ccaaagctcc agtttctcgt
    13261 tgtcgtagtc cttggacaag tgcttgaacg tctttaatca tttggttaga acacttccac
    13321 gatttcaaaa aggaacgaat ctcattttct tttagatcca gttggctgat caataatgcc
    13381 catgcttggc tctcttcttt caatgggacg gatggtaatt cagcaaaacg taaaagtgct
    13441 tcgccttctt ctttcaaacc gggacagcaa atgtagcact ctgtttcaat aaatgcatgg
    13501 attcccgcgg cccgttcatt tcccaagagc atcttgatga attccacatt tatacgttct
    13561 acagatattt tacctaatag ctcgtggttt tctttgatgg cgtagaaggt ttcttcctct
    13621 aatttgaacc ctagttgact gacaaaacgc aacccgcgca tcattcgtaa ggcatcttca
    13681 tggaaccgct catgaggatt gcctacagct cgcaagatct gttcttctag atcttgcaga
    13741 ccatcgaaca aatcgatgat ttctccatct tctttcaaag caaacgcatt gatcgtaaaa
    13801 tctcttcttt tcaaatcttc tgccaacgaa cgaacgaatt cgacatgatc tggtctacgg
    13861 tagtcctgat aagttgattc cgtccgaaat gtggtgatct catattgttc atcattttct
    13921 aataccaaca cagttccatg ttcgatccca atatctatcg tttttggaaa aagctgtttg
    13981 atttcttctg gaaatgcgct tgttgcgata tccacatcat ggattggctg gttcagcaag
    14041 atatcacgaa cacttccacc tacaaaataa gcttcatatc ctgcattctc taattttttc
    14101 agaactggaa ttgccttttg gtattccaga ggtaattgtt ttaatttcat ttttccaacc
    14161 tttcctgcgt gtttcttgtc ctgagataaa gactcagttg catggtgact gtttgacaag
    14221 tccgtttcag agtcttcaag gccaggacca cttcatcttg tttactcgtc cttacgctca
    14281 aagcgattat ggtctctttg attgaatttt tccatgtttt tttcaaaaac ttcagttaag
    14341 tcgatattca atgaattagc catgatcatc gtaacgaata aaacatcacc aagttcttct
    14401 gctaccgagt taactggttc agaagccttt ttgctttttt ctccataagt gtggttgact
    14461 tctcttgcca attcacctac ttcttcagtt aatcgagcca tctgagctaa tggagagaaa
    14521 taccccacct tgaattgctg gatatattca tccacttcct gctgcatact gtataaagaa
    14581 cgctttttat ccatcttttt ccctccaaaa ctatcttacc aaaactttaa cgcaaatcct
    14641 agataagagc aattgagaaa atcgaatttc catgctacat ttaggaagga atttctaaat
    14701 ggaaattctg tgaaaataca taattgaaag ctggaattca tatggtcaaa cgaaaaacag
    14761 taaaagaagt tctttttatc attataggaa caagtattta tgcatttggt cttgtttact
    14821 tgaacatcgc taatcattta gcagaagggg gagtctcagg gatcaccttg atcttaagag
    14881 ctttatttgg tattgatcca gcttatacga ctttattaat caacatccca ttgattttac
    14941 taggaggaaa gatcctcggt aaacgttcct tggcctatac tgttctaggt acactttctt
    15001 tgtcattctt tttatggatc tggcaaaggg ttccactaga aatcaattta cagcatgatc
    15061 tactgatcgt ttctctactt gcgggtttag ttgcaggtgt aggaagcggg atcgtctacc
    15121 gcatgggtgg aacaacaggc ggcagtgata ttatcgctcg gattttagaa caaaattacg
    15181 gcatcactat ggggcgttcc ctattggcat ttgatatcat cgtcttactt gcttcattaa
    15241 cctatatcga cttgaaacgt atgatgtata cgttgattgt ttcgtatgtt ttcagtcgtg
    15301 tgattgattc gatcttagat ggcggatatt ctgcaaaagg tctacttgtc gtctccaaca
    15361 aaagcgaaga gattgcacca ctactgatga ccggtctgca aagaggtgtg actttccttt
    15421 ctggagaagg cgcattctct ggtaattcaa aaaaaatcat ctatatggtc gttagctcta
    15481 gtgaattaac tgaagctaag aggatcgtcc acgagattga cgaaaaagct ttcctttcaa
    15541 tcatcaatgt gcatgaagtg gaagggcaag gctttactta tttgaagcca cagacaagat
    15601 tactgaaaaa actataagaa aaaagacaaa gataggctat gaggaaagat tccacatcca
    15661 gctgaccttt gtctttttta gtactatact atttttaact aattgttatg ttcacttacc
    15721 cggttacttt tcttttgtgt ctgcaaaagg atctttcggt taacttcatc agataatttc
    15781 aaaaaagcat ccctatctcc atcttctagt gcttgatcga tttgagcata aagcattttt
    15841 agttttgcct cttcttcttt ttcttgaaaa tatcgttcaa ttaattcagt atcttcttca
    15901 ctgactgaag aatcccaaga agaaaatgga ttgtcttcta agatagatag atattctctt
    15961 gtctgccaag cattttcgaa tatacattcc acatataaag gctttttcca attcaaacgg
    16021 atttcatgaa aaatctggtc tgtgtccgta aattcttttc cctgcaaaaa caatttcatg
    16081 ggttcccctt caacagatag atcactgatc tgcagtcctc gatccgcaaa attcgcccct
    16141 tcaacaaagt ggacattgct taaaatcgct tcatgattgg caagataatt caagatccaa
    16201 aaaacttctc ttcggctaaa agatacatga ttgaccatcc acgttaaaaa attcttcttt
    16261 tcccgtacat caattagcat ttttctcacc tctctgttag gtcatctaat aatgacagtg
    16321 cctccatatc tcctggttca agtgttaaat aataagtcaa taattcttgc gctcttttca
    16381 gctgcccttc ttctcttaag aaaaaggcat actctttcaa gaaatctggt tcatgtgata
    16441 gttcatgata agcctgttca tagtgtacag ctgcaacggt aaagtcttct aattcattat
    16501 aagcatgggc taggttccat tctgcataag gattagcagt ttcttccatc tgattgatca
    16561 cttgtatcac atcttcatat ctttcatcat ccaagtaaag attacttaaa gtaagtaatg
    16621 tttcatcttg tttgtctcct acttctaaag ctttcagtaa aagttcttct gctcgctgtc
    16681 tatcatgcag acggaaagca ttttccgagg ccaggtgata aagttcgact tggtaaggat
    16741 tttctttgat tccttcttct aacactgttt gtgcttcttc aatcatttct tcttcttgaa
    16801 gagcttgtcc tagatataga tataacgatt gataatgcgg attaacttct cgaagttctt
    16861 ccagatagtt gattgcctgc tgattttctt tcaattgaag attcgtaaaa gctagctgga
    16921 acagtcgatc atctgtcctt tcttctttca gtgcttcttc taaaggtacg atagctgctt
    16981 cgaattctcc ttgcatactt aaggattgtc ctaaacgttc ctgcatcgag attcctgaaa
    17041 tttcatttat cccgcttgcc aacaatgttt cataaaccac tgctgcctct ttgaaacgat
    17101 caactgaaaa atacaattca gctagcgcaa actggatcaa tggttcatca ggaagcaacc
    17161 cagctgcttc tttcaatttt gcttcactca cttcagggat tccgataact tggtacaaat
    17221 cagctatcgc caataagctt tgcggatagt aatcactttg cttatccact ttttccaggt
    17281 attcaaaagc taagtcgatc tcattatctt cgattgcaat ttctgctaac gggatgttca
    17341 gtccgtcatt ttctgggtat tggttgatca gttgttcaaa aatctgtttt gcttcgatca
    17401 aaaagccaat agccagcaat tcttcgccta gttcagctaa tatatctgct gggtcttctt
    17461 ttaatgcttt ttctaaaaag atttgtgctt gtgacaagtc ttcatctgat aaagcttgca
    17521 acattttttc gctatttgta ttcatttcaa ttccctctta tctcactgac gatttgttga
    17581 aataccgtca ttgttctcaa taattctttt tcgtttggat gttggtatcc gtctattata
    17641 cctatttttt ctaaaacaag tatttttttg cttttttctg ccaaaaaaag aactttttcc
    17701 aaatactcca tctttgaaat ctgttccagc cagtggattg gatagccaag caagtccagc
    17761 cacacaaaaa agttttttag ttggaaatca aaaggatatg cttggagatt ccacacgaaa
    17821 tgaaacatca ttcctaaaaa ttttttcata ctagcagaaa gtagatggcc attttcggtc
    17881 agataaaaag cttgttcaaa cagtttacca taatttgaca ggttttctgc ctctaattga
    17941 taaaaataga tcatttcttc gatcatcccg gcaaacggcc gagtcgttaa ttgcttttta
    18001 tttggatagt ttttatacag attttgcaaa aaacgatgat cacaaatcat tccgcaacag
    18061 ataaaagtca aaaagtcaat ctgctttccg tccacctgcc gctcgctgat tgtttgatca
    18121 taaacgatgc gttctggcag attgatggtt tgcagcacta cacgatttgg tagctgcaaa
    18181 atcgtccgct gcggtgttaa tgctcggctt agagaacgga tcgatacagg gatcatccat
    18241 agtgatgaat caagggttgt atgtttttga aaaaaacctg ccagatccat gatcccttca
    18301 tttccaaatc cgatgataaa catggattga ttttcgggat agcgtttgac gaaatcaagc
    18361 aggttcttta attctgttaa atgattgcat tgagtgttgg cacaaatata ccagtcattg
    18421 tttgcttgat ttgcaaataa cgggttgatc ttatctgcaa acaaatcata atagcgctga
    18481 ttcgttaaaa ataagattgg ctgatcaatg gaaagttctc ttagctgact gactagcgtt
    18541 tctccgtaaa acactttcgt caacgcatct tctacttgat aatcaagttc catacgcatc
    18601 cctcctcttc ttatttttat tttaccatta gtaagcaaat tctagaaaag ataagagcaa
    18661 agctcacctc ataacttgca aataaataaa aattaaataa aatacatgtg ttttctatgt
    18721 atgacatacg gattcggaat ctctgtctct tatttattcc ttttctgcta ggaaataaaa
    18781 agaaaagcac ttgtagccct agagccacaa gcgctttttc aactgtctta tttaacagcg
    18841 tcttttaatg ctttacctgg tttgaatgca ggtactttgc tagctggaat ttcgatttct
    18901 tcaccagttt gtgggttacg acctttgcga gctgcgcgtt cgcgaacttc aaagttacca
    18961 aaaccgatta attgaacttt ttcgccgtta gctaaagcat cttgaatagt tgaaaataca
    19021 gcatctactg ctgcagttgc atctttttta gttaaatcag tagctgaagc aactttttcg
    19081 attaattctg ctttgtttgc catggataga ttcacctcct ccacaagaga tatgatagaa
    19141 atatcattta gttaaaactt tctgagtggt ccagaagttt aaaaagaaat attgattaac
    19201 ttcaatattc tgttatcacg atatcatatg aaccgcttaa tagcaaggtt tttaccaatt
    19261 ttcacatgct ttttttagtc atagaaacgt aacaaacgaa actgtctttt tcttttgaaa
    19321 aaaaaacgtc gatatggtaa aatgcctatt ttctcctacg agggataatt cggatggacg
    19381 tcccttcaaa agtaaatgct tttctgattt gattttctaa gaaacgttca taagaaaaat
    19441 gcatcaattc ctcttcattg acaaaaacga caaatgtagg tggtttgaca gcgacttgtg
    19501 tagcataaaa aatcttcagg cgttttcctt tatctgttgg tgttggattg attgctacag
    19561 catccataat gatatcattc aacacagccg aagagatacg aagattctgg ttcatactta
    19621 cttgttcgat catttcaggc agacgttcca accgttgttt agtcactgca gaaacaaaaa
    19681 cgattggagc gtaatcaaga taccggaatt catcacgaat ctcttgttcg aaatcacgca
    19741 ttgtattggt ttctttcttt acaagatccc atttattcac gacgataata attccacgac
    19801 cagcttcatg ggcgtaaccc gctactcgct tgtcttgttc ccgaatccct tcttcagcat
    19861 tcagtaccat caaaacgata tcagaacggt caatggcacg cattgcacgc atcacgctgt
    19921 atttttcagt attttcgtat acttttccgc gtttacgcat accagctgta tctatcatta
    19981 agaatttctg accactttca gaaacgaagt gggtgtcgat tgcgtctcgg gtcgttcctt
    20041 caatatccga aacaattaca cgttcttctc ctaaaatcgc attgatcaaa gaggatttac
    20101 caacatttgg gcgaccaatc aaactaaact taatcgtgtc gtcgtcctct tcttcagtct
    20161 cttcagagaa atacttcact gcttcatcta aaatatcacc aatccctagt ccatggcttc
    20221 ctgaaacagg ataaggatcg cctagcccta aggaataaaa ttcatagata tcgtttctca
    20281 tttctggatt atctacttta ttgactgcta aaatcactgg tttattactt ctgtataaaa
    20341 ttttcgcaac catctcatct gcgtctgtca cgccttcgcg tccgctgact acacaaatga
    20401 ttacatctgc ttcttcaatc gcgatttctg cttgatgctt gatctgatcc ataaaaggtt
    20461 catcgccaag atcaattccg cctgtgtcga tcacgctgaa ttctcgacct aaccattcac
    20521 cttttgcata aatacgatca cgagttactc caggagtatc ttcgacaatc gagatacgct
    20581 caccagcgat gcggttaaaa atcgtagact taccgacgtt cgggcggcca acgattgcaa
    20641 ttgttggatt tgccatatta tttcctcctc acaagtttcc aatctcactt agtttaacga
    20701 atgcattgcc aactttcaag catagattgt caattaaacg aagtattttt attccttatt
    20761 gaaatactaa tcgcttttca aaagaaaaac tgagacaaaa gcagctgctt tcatctcagt
    20821 cacagataca aaatcaatta tttttcagaa tcatcggttg ctttttcttc tacttcttct
    20881 ttcaacgcat cacctaaaat gtctcccata gtaaatccag tattttcttc tggtaattca
    20941 taagactcaa cattttttgg ttcttcttct gaagctggtt tttcttctaa agctttgata
    21001 ctcaatgcaa tacgatgttc ttctggatgg atctctaata ctttcacttt cacttcgtca
    21061 ccttcatgaa gcacttcatg tggtgtcgca atatgtttat gtgaaatttg agagatatgg
    21121 actagccctt ctactccagg gaacacttca acaaaggcac cgaagcttgt caaacgttta
    21181 actgttccgt ctaatactgt gccaactgga gcttttgtat cgatttctga ccaaggtcca
    21241 ggctgtgtgt ctttgattga caaggaaaca cgttcttgtt caggattgat agacaacact
    21301 ttgacattta catcatcatt gacagccaat acatcacttg gttttgctac atgactatgt
    21361 gaaatctctg agacatggac tagaccgtca attccgccta ggtcgacaaa tgcgccaaaa
    21421 tcagtaagac gtgcaacacg tccactgaga acatctccct catggatagt tgaaagaatt
    21481 tcttttttct tttcttctct ttcgttttcc actaccgctt tatgcgataa gatcaaacga
    21541 ttttctgatg gttcgatttc gatgatttta aagctcaatg ttttgccttt atagtctgaa
    21601 aagtctgcaa caaaatgatc ttccaccatt gatgcaggga caaaaccacg tacaccgacg
    21661 tcaacgacta aaccgccttt gacaacattt gtaacaggtg cttcgatgac cttgccctct
    21721 tggaaatctc tttcgatatc ttcccaaact tttttagcat ctaaacggcg ttttgaaagt
    21781 agatagctgc cgttttcttt atctttgccg atcgtactga taacgactaa atctaataca
    21841 tcaccgactt ttactacttc gctgatatct tctaccggca aagttgataa ttcttttgcc
    21901 ggaacgactc cttcaactcc agccccttcg attccgacaa tcacttgttt gtcttcaatg
    21961 actaaaactt cgcccttaac aacatcgcca acttttactt cttgtacgct tttcattgcg
    22021 tcttccattg actggttgtt ctcttgattt tgatcaaatt ctgtcataac acttgtcctc
    22081 ctaaccaaca ttctgccgta agtacggtac tgtttttagt ttaattgatt attcaaaaaa
    22141 aataaagcga tttcgttcgt tttccttgaa aaaacaattg tttttcattt tttttcttaa
    22201 tttaaaaaag aagataacct tttttcaaaa caacagcttt tattgctgca acgacttctg
    22261 gaatcgattt gccagtcgta tcgatacgaa cagcatcctc tgcttgcttc agcggagaga
    22321 cgtcccttgt tgaatcataa tggtctcttt cctgtatctc tttcgtcagt gtttcaagat
    22381 cagtcggaat tcctttttct tggttctctt tgaaacgtct ttctgcacgc tcacttacgc
    22441 ttgctactaa aaagattttc acttctgcat tcggtaatac agctgtcccg atgtctcttc
    22501 cgtccatgac aacgccaccg cgttccccta tttttcgttg gacagcaact aattcttctc
    22561 tgacttttcc atgtgcagag acttcagaaa cagcttttgt gacatctggt tgacggattt
    22621 ctaaagtcac atcttttgat gctatgaata catgctgtcc atcttcttgt tgcgaaaaag
    22681 agataggata ctttttgatc agcgaaacta gcccttcttc atctgaaaaa gatacattgt
    22741 tcgtaatagc taaataagtt actgcgcgat acatggcacc ggtatctgta taaatataat
    22801 tcaattcttt tgctaatatc ttggcaacgg tacttttccc agatgaagca ggcccgtcga
    22861 ttgcaatact gatttgtgtc atgatataac tcctttttct cagaaaaaaa gccgcaaagc
    22921 gcggctatta ttatttaatt cttaattctt gaccagggta aagcataaag ttgtttggat
    22981 ctaagccatt caattcaaac aattgatcag tggtgatccc tgcacgctca gccacttgtt
    23041 ttggaccttc gcctgcttga acagtcgttg ttgaaccggc tgcagctgat gaagaaggtg
    23101 ttgtttctgc tgcattgctg cttgaatccc ctgcttgata agttgcctca cttgaggaag
    23161 tcggtgttgt ctctgctgca atactgcttt ctgacgttac acctgtatct tgagacacag
    23221 tagattcttc cgcgattgta ctagattcta ctgttgaagt ctcagttgac tcctcttttg
    23281 ttgtacttgt agaactttct actactgatg tcgaagtttc agaagaagtt gctgctgttg
    23341 cattgttgct tgatccattt ctgatccaga aataagcacc tgctggaata gcgatacata
    23401 agaccagtaa aatcaaaaat agcgaaagaa ataatgtatt tcctttcttt tgttgtctct
    23461 gagaacttct tgatgaaact tcatcatctg tatcgtagat tggttgttcc catggttctt
    23521 gtgtttcgtt attttctgta gattgatgtc tatcttttct gctcactgtt ttacctccta
    23581 aactctatcg tgtgtattat accataggaa aaagtgtctt gtcctaaaaa atcaatgttc
    23641 catgtttttt ttaaataaat ttagtaaaat cgttccccaa tgaagttgtt cagtcttaga
    23701 aaaaagcttc ctctttggca gaagttctgc ttttgctcca tcaatatcac aacaattctg
    23761 cggcttttcg gagagttttt cgccaaaata agctaagatg aattttctgc ggcagttttc
    23821 ttcattaata tagcgaagca tctgctgcag gcggaattct ttttcttttt cctgacgttt
    23881 gagccgttcc acccaatttt ctggtttttc agactgctgg atcagctcca tccatttttt
    23941 ttgcagctca tcaaagggag cttgctctgt ggcatattcc aaataaagct caaaactttg
    24001 tcgctgttct ctagaaagtt gattaaagaa ataatggata cgttcatccc cactttgata
    24061 aaaaagaatt gctgcacttt cttcctgatc tctgcctgct cgaccgattt cttgtacata
    24121 gttttctaga ctgtctggca aatcataatg gaccacataa cggatatcag atttatcgat
    24181 ccccatacca aatgcattcg tagcaatgag aaactgcagc tgatttttta caaactgctg
    24241 ttgaagttgt cttctttgag ctgcatcaag accaccgtga tagtatccaa ctgtaaaacg
    24301 ttccctgaat aaatgataca atcgttcgac ttcttttttt gttgcacaat aaatgattgc
    24361 cgctccttgt gctctttcca tgaagtgttc aagttcttgt tctttttgct gcgttttccg
    24421 aacaaaaagc gagatatttt gccgattgac tgattgtctg atctcttttg gtggtcgatc
    24481 aaatagtaca tgacgtatat ctcttgctac taaatctgtt gctgtcgccg ttagtgccag
    24541 tgtgaccggg tttcctaaaa gctttcggat ttttcctaac tgttgatatt ctggacggaa
    24601 atcaactccc cattgagaca cgcaatgagc ctcatctact acatataacg caatattttg
    24661 ttttttcaga tgctctaaaa gagaaggatt agtcaacatt tcgggactca aaaacaaaaa
    24721 tttatattgc gatagatgac ggaggacgta tcttctttca gattgcgaaa gtaaactatt
    24781 gaaagcagct actcgtttct cccctcgttt ttgtaattgc gtcacttgat cttccattag
    24841 agaaagcagc ggtgacacga tgatgaccag accttccatt aaataccccg tcaattgata
    24901 gcaaaggctt tttcccgttc ctgtaggcaa aatagcgaga gtatcttctc catcaagcaa
    24961 cgcctgaata atttcttctt ggccgggacg aaatgaatca aatccaaaat atttttttaa
    25021 ttcatttatt aacattctgt atcgcttcct ttctcaatag atagatttga tacaaacgaa
    25081 attctccata atctaattgt ccatctacat tccattcgct aaagcgccac tctcgcacgg
    25141 aatccttatt tggttccaaa cgttcgattg tttctgcaga gagaatccac tcgtatggaa
    25201 aatcatcaaa aaacaaggcc cactcaataa gatgatctgt tactgttcct ctttttaaat
    25261 gacggattgc catgatctgg tcaattgtct gaccattcat aaacatctgc ttggtgatca
    25321 tcatgctttg attgaaattt tgctgtaata atgaatcgat caaagcgtgc caaagcgaat
    25381 ctggccgttt ttcgatttgt gctaacaaaa ggtggataca tctgctttgt tgcagataaa
    25441 ccgctgactc atcattgttt tttgccagct gataaggaag cagccctgtt cgctcatttc
    25501 cagataattg gttagctaaa aaatctgctg cttcttctgg tatctccgaa aacagctgca
    25561 ccaaactctc atacgcagta tcaatcaaaa tttcacgtgg caattggctt tcactaagcc
    25621 atttttttag ttggaaagta taaaaaggac ttgtctctgc tggaagatag tcttgaatac
    25681 ccgttgccag attagaaatt acttgaacag caaatttaat gagccgccaa ctagtttccc
    25741 ctgttctgcc atagcggtca aaacgcaaac cagtatgttc tatttgatgt tcggacaaca
    25801 tatcagcccc tgcaggcgtt agctttgctt cattttcatt tatttcgatc caaccttgct
    25861 gcgcaatttt ttgcaaaatt tgataaaact tatcctgctt caattctgga aaactgttat
    25921 ggataaacaa aagttcgtgg gtaaatccat aaataagtac tgaagtcgtt cgttttccca
    25981 caaggagttg gtaaagagaa ctaatccgca gcttgtgtcc tgatgaaaat agcgataaaa
    26041 taaaaacaat catattattc tccttaagtt aggatggtta gaaatgtcac tactatgtaa
    26101 acttgtacct gaacgctgta ttgcatgcgg tctttgtcaa atcgaagcgc cagatatttt
    26161 cgattatgat gaagaaggaa tcgttttatt tgcccaagaa ccagaggctc atcaacagtt
    26221 cgtgatgccg gaagaagaaa catatgtaaa aaaagcgtat caaaaatgtc cagttcgagc
    26281 gattttatta gaaaataaaa tataaatata aacgaaactt taaaaacgta cactaagttc
    26341 tctaaacttg aaaataagcc gggatctgtt tctcttgttc cgtgaaatcc cgacttattt
    26401 tgaaagattc atttgctatc tatagttaat tgactagttt tttcacggac cctataggac
    26461 ccatttatat tatttcaact ttttcagcca gttttccaat gtattgacga tcacagtttg
    26521 ctggtctgcg ttagaaattt ctgcctctcc atctcctttt tgctcaccat agctgccaaa
    26581 acctccatga tttccgccat tgattgatac atactctgtt ctttccggta aatactgctt
    26641 agcttcttga taagcttcct gattcaatac tttatctttt gtcgccgtga tcgataaaac
    26701 aggaacagct gattctgtca atgttccttt tttgtcggga taacttgcca tgaagaatac
    26761 cccttttaaa tgtttgtctg aatgattctt attgacataa cgacttgcca ttacgccacc
    26821 taatgaatgg ccaccgatca cataatcgga atttggaaaa tcttctttga ccttatccgc
    26881 tttgtttccg cccatcactg ctagatcaag tggcattttg acaatagcga ctgaaaagcc
    26941 gttttttgct aaatcttgcg cccaaatact ataactttct ggctctacga gagcaccagg
    27001 ataaaaaata agtaatggag actttccatg attctttttc tctccttcaa aaaataagta
    27061 atcttctttg ttttttcctt cttttaccaa cacttgcgct tctgacgatg gttcgtacgt
    27121 attttgctgt atatagtaat aaccggctcc accgagtaat cctaaaatga tgactaagcc
    27181 aattagtatt cgtctaaata ttttcacttt ctcccacctt ttctatctga gtgacccctt
    27241 tttcttaatc aagtatgatt ataccttaaa aaaataagag agaaatgcaa actttccagc
    27301 atcaaataaa aaaagacgga atatccattt gaatatccgc cctttttgta tgtttatttt
    27361 tatttagcac ataaacgata cacagcttct gcataaattt ccattgcttt atacatacta
    27421 cttaattccc atcgttcatt cgcctggtgc ataaagtcag gagtatcagg gaacatcgca
    27481 ccgaatgcca cacactggtt catcgtacga gcaaaagtag cgccgcctga tacaaacggt
    27541 tcagtcatgt ctccagtaat atcgcgatat gtccccaata gatttttcac taactcactg
    27601 tctagtggca cgtataatga atcaaggaag tcaaattctt cataagtcaa cccatatttt
    27661 tctgctgttt tagttaattt agctactaaa tcatctttct tgaatgtaac agggattctc
    27721 atatctacac cgatttttgt ttgttctggt gtgatttcca aacttgcaaa gttcatcgtt
    27781 agctcgccgg attgttcatc ttctgttttg cccacgacag cttcaccagt tgcattttct
    27841 tgaactaatt ttcctaagaa attgattggg ccaaaatcaa atacttctga aagagcaatt
    27901 gccagacgag aaatagcatt cactccttgt gctgcatctt tcgcgtggat acttttacca
    27961 agaacgacga tgccttcacc ttgttcttcg aaatcgaatt cgtgtttttt caatgcttct
    28021 tttacttcag aaagcttttc tccagagtaa ggagctgcat caggaacaac attcaacgct
    28081 cctccagctt ttacagaaaa ctcgttcgtt cctggtcctg tcaagtaagc ttgcaataag
    28141 cctttttctg cataaattaa aggaaattca gcatctggcg caaatccttg cgtaattcct
    28201 tcttcttttt cgttatattt ttctaaacaa cgccataaat tttcttcatc tgtaccaaag
    28261 ataaaacgaa tacgtgtctt gaattccacg ccagcatcaa tcaaagcttt gacggcatac
    28321 attgccgcaa tagaaggacc tttgtcatct tgggatccgc gaccaactaa ccagccgtct
    28381 tttatagttg gatcgaatgg ttttgtttcc caattatttt cgtcccctgc tggaacaacg
    28441 tccatatgac acaagatacc gaataattca tctccagagc caatctctga ataaccgtaa
    28501 tagccttctg gatctttaaa tgttctaaat cctagctttt cactgatttc caatacttta
    28561 tccaaagctt caatcacttt tttaccgaag ggatgtcctt gtcctgaatc tgcttcatct
    28621 agtacggaag gaattcggat caattcgctc aatgattcta atgcttgttc atgatgttgt
    28681 tctgtcacaa attttttcat gatattatcc tcctaatatg ttaaaaaggg tcatgtcaaa
    28741 ttcctccgac acaacctctt cttctcaaat aatgacgttt taagcctgct ccatcagcaa
    28801 gcagaatgtt cttaacaaat gatgaaagaa aaaacactca ctgaatggag ctattgactt
    28861 atccacgtgc ttatcgatag ccgtttatcg gtttcttcat tcttatgata gaccttgtac
    28921 ctgtatttgt ctaggtttag aacattgctg ctacacctag gatcacacag atggctacaa
    28981 ataatatcgc aattaattta ccggtaaatt tccaccacac tgaaacatcg aaacgagcaa
    29041 ttgctacagc acccattacg atagcagatg ttggtgtaat taagtttact aaaccagatg
    29101 cagattgata agcagtgatt actaaatcct taccgacgcc ggcaaattca cccataggtc
    29161 ccatgattcc cattgttgca gcagctaagc cggaagttga aggaatcaag aatgacatag
    29221 ggatatagaa aataaatgta ataatgatga atacacttga agataatgaa cttaatcctt
    29281 gttcgcccca atgtaagatg gtgtctgtga ttaatccatt gttcataacc acttggatac
    29341 cacgagcaac agctactacg attgctacac cgatcaaatc agaagcacca gctaaaaatt
    29401 cagagacaaa ttcagactct ttcatgccat aagtgattgc tactacgatg gacattacta
    29461 agaataacat agtaatttct gggaagtacc agtcacctaa tggaagcatg cttttaccta
    29521 gaaccgtacc tagtaccgga aggtttgtta accagtttgt caatgattca aaaatcgtga
    29581 aattcgcatt caaggttgtc caaggaatca aactgatgat catgatacca aaagtaataa
    29641 agaacaatac attaacatgt ttttgttttt tcgtgattac ttcttgacga ccgattgctt
    29701 caatatcgaa atgcttcata tcttctttac gttggttgta tactaatgat tttgttggat
    29761 ctttttcgat ctttgatgca taacgataca catatacaat agaaatagct aacaaaataa
    29821 cgaacatcaa gaatcgaaga ccaatacctt cacccatcga aatcccaaca gcttgtgaag
    29881 ctacgcctgt tgcaaaagga tttaccgttg aagctagaca gcctacttga gaccctacta
    29941 atacgattgc tacagctact actgtatcaa agcctacagc catcatgact gggatcagta
    30001 aagcatagaa tggaatcgtt tcttctgcca ttccgtaagt agagccgcct aatgcaaata
    30061 acagcatcaa tactggaata agcatttttt cttttccttt gaatttcttc acaactgaag
    30121 caatcccggc atctagtgcc cctgttttgt tgataatacc taaaaatcca ccaacaacca
    30181 aaatgaagaa agagattggg attgcagcag gtgtttcttt agttagctcc cccgctggta
    30241 cacctagcat cccattgatt ggcgccatga aaatatccca cagcccttga ggattttgtt
    30301 gtactcgttc atatgatcca gcgacgatgt tgcctgcatc gtctacacta tagtgcccag
    30361 ctggtataaa ccaagtaagt acagcgatta ctgcaataat aataaataag accgtatacg
    30421 ctgaaggcat ttgaaatttt ttcttttttg tttgttccat cttcttcttc ctttcttttc
    30481 cgttagagac aacaattttg gctctaatgt tattttatca aaaagagtat cagaacaaaa
    30541 ataataacac gcattttgta aaacttttgt tcaaaaagtt cgtttttccc tattccctcc
    30601 tcttttttca ggtttttaaa gctttcttct ttttcattgt agcgtttcct tttttttata
    30661 aacaaacatt ttcgtctttt aaaaggagaa gaatttcaca tttgcaaaga attgattgat
    30721 ttttatgcat aaatcaaaaa aatactcaaa acaaacaaat aaaacgttta cattattgca
    30781 tgtcttgtat ttttttccaa aaaaaaagac caagaaattt cttgatcttt ttgatattta
    30841 tttgactgct gtatgacgtt gttcgaactg atgttgtttt cgacttaacc atggaagaag
    30901 ttgcgcatgc agcactaaaa agacagcact cacgatgaat cctttgatca agttgaaagg
    30961 aacgatccca atcagtacat agtttgctaa agacatgccc agcatctgtg ttgcgttcag
    31021 ccctaaaaac attaagtaaa gaggcgtaat aacgaaataa tttgcgatac tcataaaaat
    31081 agttagagcg atggttcctg taatgacacc taaaaatttg tttcttcctt tatgctgttt
    31141 ctttacacta aagaaataat aaatcgggaa tgtaaagatc gttgttgata agaaactagc
    31201 agtgtctcct accaaattat ccggagaaaa acctgtagtc agcagatgta atactgaacg
    31261 caagaacgct gttgcaactc cggccgctgg accgaataag aacatgctga tcaataccga
    31321 gatatcacta aaatcgatct tcaaaaaact aaaagccggc atgatcggaa aagcaaaata
    31381 ctgtaaaact aatgcgatcg cagcaaacat cgctactgca accattttct gtacacgggt
    31441 gttcttcatt tgaaatcctc ctgttaggac tcctcttcag cgaacttttt gacgtttctc
    31501 tccaaatgaa aaaactctat tttccaggga aaatagagtt agagttattt gatcaagcgt
    31561 cgtcaaataa gctctatctt ctttatccag actatactgt cggtatcgga atttcaccga
    31621 ttcagctatt tttttgcaaa ataggtcgtg gactataacc accggtcggg aatttcaccc
    31681 tgcccctgaa gacgaacctt atataatttt tgtttacata ttcattatac atatctattc
    31741 aaaaaacgca acatctaaca cttataaaaa gtaagtgttc attttgagtg gttcagcaaa
    31801 ctgctgactt cttttttgtt cagttcacgg taatccccag gacgtaggcc ttgtaaagtc
    31861 agatctccgt atttttcacg ttttagtttt tgcacaggaa gaccaactgc ttgaagcata
    31921 tttttcactt gatgattacg tccttcgtgg atcgtcaatt ccacgatact gtggtttgtc
    31981 ttaggatcca ctgataaaat ttggaattta gctggagatg tttttcgtcc ttcgatcttt
    32041 attccttttg tcaaaggtgc aagcatcgat tttgtagcga tcccttttac ctttgcaacg
    32101 taaactttat ccacttcatg ttttggatgt gtcaggcgct gagcaaaatc tccatcattt
    32161 gtcagtagca agatccccga agtatcgtaa tccagtcgac ctacaggata gatacgttct
    32221 tttaccatcg gcaaaagatc cgtaacaact tttcttcctt tgtcatctgc aacagcagaa
    32281 ataactcctt taggtttgta caataaatag taaccgtatt cttcttgata aattgggact
    32341 tcatccactt ccacagtgtc atgtttccca acttttatcc ctaattccct tacaacttgt
    32401 ccgtttactt ttacacgtcc tgcaaggatg aattcttctg cttttcgacg agaagtgatg
    32461 ccagcgtggg caattacttt ctgcaatctc tccattacaa ttattctcct ttattcattc
    32521 catcaaagaa tagatctggt gacacgtctt cttcaagtga aatttccatt tcttgtgtat
    32581 caggcagttc ttctaatgat ttcaaaccaa aataatctaa gaagtagcca gttgttccat
    32641 ataatatcgg ccgacctggt ccttctactc tacctttttc ttcaatcagt ttatgggcag
    32701 ctagacgctg taccgctcct gaaacctgaa ctcctcgaat ctgctcgatt tcagctcttg
    32761 tgattggctg tttataagcg atgattgcta aggtttcgac ggcggcttga gataatgtgt
    32821 ttgatgtccc ttgcgcatat cgcttaagta agggagccag ctctttttta gtcgttaata
    32881 cataatggct ttccgtttca aaaatatgca aagctgagtg cgagttttga gtgtaagatt
    32941 cttttaattc tttcactaaa cttgtgactt ctgcttcgct gagatttaac agtccgctta
    33001 attcttcatt actgatccct tcctcgccaa caataaaaag aagggcttca agttcactta
    33061 atttcgtcat gttcggtatc tcctactgct ggataaagca taattgattc atagttttct
    33121 ttttgttgcg caatgatctg cccttttttc atcaattcta gcaaagccat aaaagtagtg
    33181 acaacttcac ttttagaagg tgactcaaaa aaagaatcga aagtacagcc atgcgcattt
    33241 ctttttatac gctcttcgat aaataccatt cgttcttcga tcgttgtgtc atcgggcgtg
    33301 atcgtttttt ccactttttg agaaaatttc ttacgttcta agattttctg catggcacgg
    33361 atcaaatcga tttttttgac ttgtcctcgc ttcaacggct tttcagcatc cgctaaccca
    33421 tcgatgttca taggttcttt tgtgtaataa agacttcgtt cctctgcttt ttctgtcagt
    33481 ctttcagctg catatttata tttacggtat tctaaaagct gagtgaccaa ttcttctcga
    33541 ggatcttctt cccataattc ttcatcgtct gtgacgaatt cttggacagg aagcaatgtc
    33601 ttgcttttga ttgccatcaa tgtagcagcc atcactaagt attctcctgc aacagctagc
    33661 tcgagtgttt tcattgtatg gatataattc atatattgct ccgtgatcgt agcgatcggt
    33721 atatcatata tatcgatttc catttgctga atcagatgaa gcagcagatc gagaggaccc
    33781 tcgaatacat ctaatttaat attgatctcc gtcaactcat tcactctttc ttctttttct
    33841 ttgctagcca ttttccaatg taaggctctg tgatttcagt tgcacggatg gagacatccg
    33901 ggtacttttc ctgtaattca tctaacattt gaaagcccat attctcaccg cgataagacg
    33961 gattcaaaga aatgtcatgg atgatcagct gatcgttcgt tgcccagtaa ataccaataa
    34021 tcccttgaat gttggttgaa ccttctggcg taaagaaaaa aagttcataa tcgtcttcag
    34081 attcgtacgt atcaatctcc ttcaacaaag aagttttacc tgtcagtttc ttatggaaac
    34141 tcaatagtcc catagcaatt ttacggtttt ctttccggta atgtgttaac atcttctcac
    34201 cttcctatga ctaagccctt ggaaaatgct gcttataaac atccgtcatt cttttcttcg
    34261 tgatatgtgt ataaatctga gtcgtagaga tatccgcgtg acctaacaat tcctgtactg
    34321 tccgcaaatc agctccattt tctagcagat gtgtggcaaa gctatgccgc agcgtatgtg
    34381 gtgttacttc tttgtaaatc ccagcctctc gtactaactg cttcagattt ttccagattc
    34441 cttgccgaga aagacctttc ccatgattat tgacaaatac atgcatttca gatgcgtcct
    34501 ttacaagtaa tggacgtgct tcgtctaagt agcgttccag ccattggatc gcataatcac
    34561 ctaaaggaat gatccgctct ttatctcctt ttccaactgt ctgtaccagt ccaagagaaa
    34621 gatgaagatc tcccaatttc aatccaatca attcactgac gcgcatccca gtagcataca
    34681 tgacttccaa gattgcacga tctcttatcc ctaacgtttt tgtcgtatca ggtgcttcaa
    34741 tcagcttttc tacttctgtc agagtcaatg tacttggtaa tttctgtgct ttcttcggcg
    34801 tatcgatatg ttgcatcgga tcatgatcag tgatacgctc ttgccgtaaa aactgatgga
    34861 atcttctgag acttgagatc atccgagtaa cagttgctgt cgcttttttt tcttcatgca
    34921 acttctccag aaaacggatg actaaaaagc gatcgacatc ctgccatgaa gcaattttct
    34981 gctcctgtag aaaatgcaag tactgttcca aatcacgttc atagctttgt cgtgtattgg
    35041 cggacaatcc ccgttcaata ttcaagtagt ggagataatc gttgatttgc tctttcataa
    35101 taatccccct catgcctctc tatcatatca gataccgctt cattctgata gtctttttcc
    35161 ataatctgag caaagaaaaa gccgtctaga cactcagacg gactggtttc ttttttcttt
    35221 ttcatagcat tcctgacaga ttccatggaa cgttagccga tgatctttta ctaaaaaatg
    35281 ataacgttct tctactactt gctccacttc aagtaaaaga tcctcttcta cttcctcgat
    35341 cgtgccgcag ttcaagcata gtaaatgatg atggaaatgt ttggcgcctt cttttctcaa
    35401 atcataacgg gctaatccat cattaaaact tactttatcg acaattttca attctgtcaa
    35461 gatttcaagg gttcgataaa cagtagcaag tccaatttcc ggactctttt ttttcactaa
    35521 aaagaagatt tcttctgcag ataaatgctc tttctcattt tccaaaagca caagcagagt
    35581 agcttctctt tgcggtgtca gcttaaaccc cgcttcatgt agctgctgtt ttgttttttt
    35641 catcgccgaa atagcattca tgaccattta ctcaacccca acaatttata ataattacaa
    35701 ataaaaaaag atataacact atgttaatta aaattattat aagctagtta agaaaaacat
    35761 gcaagggatt ctcaattaga ttttttgact agttttgtca caccgttttc ttgtgtaatc
    35821 aatccttgtt tcaaaagatt acctaatgcg cgcttaaatg ctcctttgct gataccaaaa
    35881 gctttcatga tttcttctgg atccgattta tctgtaaagt tgatttgctg atctgcagaa
    35941 cgttctaaaa tggttaaaat catttgtgca tcatctgata tagcttcata tgctcttggc
    36001 ttcaatgaaa gattcagtac accgtctggt cggacaccaa tcactcgacc ttttatctgc
    36061 tcgcctagac gaggttcatt atatcgttca gaaggatgga taaacccaag atagaaatct
    36121 tccgttaaaa cgtacgtacc tgttaatttc aagcggtatg ctgttcctat cacatctttg
    36181 tttttcattt cttctgttcc atatttgctt aaagatttga atattttttc atctgctaaa
    36241 ctgccccaaa tacggtcttt tgcatcaact ctaagggaaa tcatcaagcg atcccctttt
    36301 ttaggccata gctctgtcat tgtcggcaat tcatcaagag atacgacaaa gtctttatct
    36361 ggaagaccga tatcgacaaa cgcacctaaa tcttttcgtg tagcagtaac gattccaaaa
    36421 gcgtaatgac cgatacggct tttaggaatt tgtgtagtga tccgattttc atgtttttga
    36481 ttttggtaac cgaatccttc aactgcttca ccgattttgt gttcaccttc tgatttgctt
    36541 aagccaaagg tttgtccgtt tttttggaca aagtagtttt tttcattttc atcaataata
    36601 atacctgtaa atatagttgc taataaatcg ttcatttttt tctccctcta tagtgaaagg
    36661 ttttcttaat acatttcttt gctatcataa cataatgtta tctacttgaa tagctgagcc
    36721 ttggtttgga aaggtgaaaa aaggagtatg acaacagttg tgtcatactc ccctttttcg
    36781 aataaacggt gttaaacggc aggctcacag cctctttcat ttgcctaaaa ttaattgctc
    36841 ccatttcctc cgaaccatcc agttttacag ttgatggtta cgatgtaaaa tggcaaagca
    36901 ctttctccaa tgcctttcat caatgcagat tttgttgaag tggcatatag attataatag
    36961 ccttccccat tttcgattct ggctttttct aatctgtaac ctccttcaac gatatatcca
    37021 cgttggatac atgtagcaat cactttgctt tggacacctg gtgcccacgt ccatgctgga
    37081 ttagatgcat cggcaatatc acttgctctg gcagatttga ttggctgatt cgctgagttt
    37141 gaagaggaag gggtcgagga tgccgcttct tgctgtgctg cttgtgcgac cgcagcttgt
    37201 tgagcggctt gagctgcagc tgctgcttga gcggcttgtt ttgcttgttc ttcagctgct
    37261 tttttcgctt cttctgcttt tttcttttcc tcttcttctt ttttctcttt atcagtgagc
    37321 atcttatcta cttgtttcaa ttgttcattc aactgagcag atagatctgt atttttcact
    37381 ttttctactg cttcttttgc cgtcttgtat tggtcgcgtg ttgctgaatc cactactttt
    37441 ccgtctttaa agactatagc tgttttttct ttagctgttt gcaattcagc atattgattt
    37501 ttagcttctg tttgtgcttg ttgaatcaat tttccaaaat ctgtgttatc cgtttcgatt
    37561 gcttggatct ctttatctgc tttcaacttg atgtcatctg ctagatgatc atcgacgatg
    37621 gcaggtgcta tgaaccactc attcactttt tggatatctc ggatcttaga ttggatatct
    37681 tggtaagttg tttctaactc tgaatatcct ttttcattct taagactgga taaatcattt
    37741 tttagttttg ataaatcttg atgaatcata tctgcacgaa taaattgatg gtgatcatct
    37801 gtataaaagg cagcaagttt ctcatgaagt tcttccattt gttgctgtct ttttgcttct
    37861 gctgctactt cggcttctac tttttgatga tggtcataat aagcccagct tcctccaccg
    37921 agtagaagta ctgctgctaa aacgagataa ggtacttttt ttgatttttt cttttgcgct
    37981 ttatctggat ctgttttttc taagtttgat tcttgatgtg ttttttcagt atctggcacg
    38041 attgttattg atttcgcttc tgcttcaaac agcttaggct ggttctcttc tttttctatt
    38101 tttgcttcaa gatcagtacc ctctttgctt acagattgat tttcagcatc ggattcacta
    38161 tttatttcat cgggctctgt tgactttttg tctgtgtttt cgagtatttc ttttttctct
    38221 gcttccggca gtttattcgt tttttccttt tctaattgtt tttcagtaaa tacttcagtt
    38281 ttttctgtga tatccgtttt tacgtctttt atttttactg tttctttacc tgtggcgttt
    38341 tctctatcag agtctttttc agtttcttgt tccttttctg gctctacttt gttttcctgt
    38401 gcaatttttt cactgatcat tttgagcagc tctttcgcgt gctcttcttt tgtatcttca
    38461 cgatgtgctt tgatataagc ggatagtatc gaattttctt cctcattatc gacgatttct
    38521 ttcagttcag ctactcttct tttttgtttt gattcaagcg tttcttcttg attctttcct
    38581 gattcttccg aagtctcatg aacagttgct ttcttctgtt catttttctc tatattagtt
    38641 acttgatcgt gagcaggtgg ttcttcgtct tctggttctg ctttttcgaa taattccatt
    38701 acagattcta gaggtaaatc tttcaattcc gaccattcga ttggatcgtt taattctgta
    38761 tcaggaaaga ctgcatcttc agttgattta tcctgcatag ctgcatctgt atttgtttct
    38821 tcaattgatt cttttatttg ctgatcttct gcagttattt gatgaccgca gtctggacag
    38881 atagtattct gctccatttt tttcgatccg caatttggac attttttcat cacttagcga
    38941 tcggctcctt tactaggttt acgctaccat ttaatctatc atattctata cgtcacgcca
    39001 atagcttatt tttaggattt acttaaaaat aatgaatgaa tttccttcct atagaaaaaa
    39061 aagcttgaga ctgcgtagtc tcaagctttt tttcttttaa aataaaccaa acagttttac
    39121 tgatttctct tctggtcaat agtccacgct tattctaaga aatcttttaa ttgttttgaa
    39181 cgagaaggat ggcgcaattt tcttaatgct ttagcctcga tctgacgaat acgttcccga
    39241 gtaacaccaa atactttccc tacttcttcc aatgttctcg tccggccatc atctaaacca
    39301 aaacgtaagc gaaggacatt ctcttcccga tcagtcaatg tatctagtac atcttccaat
    39361 tgttctttta gtaactcata agctgcgtgt tctgctggac ttgttgcttc ctggtcttct
    39421 atgaaatcac ctaagtgaga gtcatcttct tcaccgattg gcgtttcaag ggaaacaggt
    39481 tcttgcgcga ttttcaagat ttctcggact ttttctgttg gaagatccat ttccgcaccg
    39541 atttcttctg gcgtaggttc tcttccaagg tcttgcagca attgacgttg gatacgtatc
    39601 aatttattaa ttgtttcaac catatgaact ggaatacgga tcgtacgtgc ttggtcagcg
    39661 attgctcgag tgatggcttg acgaatccac catgtagcat aggttgaaaa cttaaatcct
    39721 ttgcggtagt caaatttttc aactgccttc atcagtccca tgtttccttc ttggatcaag
    39781 tcaaggaact gcatgcctcg tcctacataa cgtttagcga tacttactac taaacgaagg
    39841 tttgcttctg ccagacgctg tttcgcttct tggtcgcctt cttcgatttt caatgctaac
    39901 gctacttctt cttccgcagt taaaagggag acacggccaa tttctttcag atacatacga
    39961 accgggtcat tgatttttac accggttggc gcagaaagat cttctgtttt tgcttgttca
    40021 gcttttttct cagcactctt caagctgtga attgatggat caccattttc atccaccaca
    40081 ctgatacctg cgtcttcaat cttttggatc aatttgtcca tttcatctgc attcaaagtg
    40141 aatggtgtag caagctgatt ggataaatca tcatatacaa cttgtcgttt gggtttgttt
    40201 tctttgatga aagctgctac tgctgcttca tacgtttttt tatctgttcc atttgccata
    40261 aaagaaggcc ccccttgagt agctatttgg cttgttttaa ctgtttcgtt aaattgatga
    40321 tttcaatcgt caattcaagc tcaagctgtt tgtttccagt tcgtctggct tcttgctgca
    40381 tttgtttttt taatgttatt tcatcagcta agccagattt gctgatcaca tctagcaagt
    40441 cttggatttc tcgtggcgaa ctttcttctg ccaccggtaa atattcgatt tccaccacca
    40501 accgccttaa attttcatct tgcaaaaagt ctaaaaactc tgcaaggttg aattcataat
    40561 gttgtgagac atatgcatcg aataaaaaat aaacttctgc atatgcatca tgaataaact
    40621 gaaattctcg ttcttttaaa atattacgga cagaaacttc tttaaatgca cgatataata
    40681 gaatctgctc tgccttttct acttgcgtca aaggtctctt tttccgtgca gtttcttgtc
    40741 tcgtaacctg agtgtgttga ctttcctgag gttcttgacg attttgctga tttgtccttc
    40801 gaagatcgcg cagttgtttt tgtatcgtct ctctgccgat ctgaaactct gttgacagtt
    40861 gagatagata catatcttgt tcgattggtg aaacaactcg gctaagttca actagtaatt
    40921 cttcgaggta ttcgatcttg tctttttcat tatctaaatt tttcccttgt tttaaataac
    40981 gtgatttaaa agtaaagact gtttcgcgcc catgagccac taattcttgg aaagatgctt
    41041 ctccatactt acggagatat tcatcaggat caagcttttc tggaacgact acgacagaaa
    41101 gtgctaatcg actgttttgc cgaagcaatt caatccctcg attggttgct tcaaaacctg
    41161 cgttgtctcc gtcatagcaa aagaccaatt ctttcgtcag cttttccatt tgttggatct
    41221 gttgagctgt tagacttgtc cccatagaag ctaatccagt tttgatacca gcctgccatg
    41281 cagcaatcac atccataaac ccttcgaaca acaaaatttc tccgtttttc cggatatcgc
    41341 ttcttgcttt atcaagatta aataaaactt gtcttttatt gaacagttct gtttctggac
    41401 tattcaagta tttgggctga tctttttcct gccctttttc aggtgcaagc cagcgtcctg
    41461 aaaaaccgat cgtctggcct tgagaattac gaagtggaaa catcactctt tgataaaaac
    41521 gatcagacat acggccatca tcatgcgtga caaacaatcc agagcgttct aacaattcat
    41581 ttgaaattga ttcattcttg aatacttgct gaagaaaaga acgctcatta ggtgcaaaac
    41641 ctatgttgaa ttcttcaatt aattctaaag acaacccacg actttgcaaa tattctaaag
    41701 ctggctgtcc agcttttgta tgcaatagca tgtgatgata gatttccgct gctttttcat
    41761 gagcagcaat cagtttcccc gtagcagatt caggattttg gacagctata gtacgctgac
    41821 gccattgatc ttctaacggt atctgttcga agtctgccac ttttaccact gcttctggaa
    41881 aacttagacc ttctagttct tgaagaaaag taaacacgtt tccgccttta ccacaaccaa
    41941 agcaatgaaa aatctgcttg tcttctgcaa cagaaaaaga cggtgtacgt tcttcatgaa
    42001 aaggacacag ccccaagtaa tttttaccag actttttcaa ctggacatat tgtccaatca
    42061 cctcaacgat attcgtttga ctgcgtactg tttcgataac ctcttgagga atccgttgag
    42121 ccattttttc accccctgct ctattgatat cttttgggtt taacagaaga tatttcagga
    42181 gaataaccgg taaaaaatcg cactaactga aatcagtgcg attttaaagg acacaattat
    42241 acatgtttga ttgtatcata tttcattgct ttttcaagta taacacattt ctatctcatt
    42301 aagcatcaat ttgctactct tttcagtaac tgattttgaa tatcagcctt ccagtacaat
    42361 actcgaagaa caatatttac gagcatgaat acaaagaagt atcctacgct ctctcccaac
    42421 cctaagttta cttccaaaat tccatggaac agcattgcag ctacatatat ccctacgatc
    42481 gtcgttactg ctaggaagat gcgcaatggg ttgaagggat aacaagcttt tacaactgcc
    42541 atgcaactga tcccaatcag caagtaatac atcaaagtag tagtttcagc atttccccat
    42601 gcgaatgttt ttccaagtag atacaccgca atgatattca ctactacaag caaggcattt
    42661 ggtaaagcat taataagtgc tgtaggcaaa taacggtaag tgatttttct tttatcttgt
    42721 tcaaaagata aaaagaagga aggataccct tcaatcgcta aatcaatcag cgtgatttgg
    42781 atagggataa acggaaatcc aattgctgta acggcacata taatcgataa aataaatgaa
    42841 taaatcgttt tgataaaaaa gatgcctgag actttcgtca cattgttgac cactcggcgt
    42901 ccttcaaaca ggacttctgg caatgtagta aaatcagagt ccaacaatac cagattcgca
    42961 atctgacgtg ttgctccatc accttctgcc atagcaatgc tgcaatctgc ttctcttaac
    43021 gccaaaacat cattcacacc gtcgcctgtc attgcaaccg ttctgccgct ttctttcaat
    43081 tcatttacca gcaatttctt ttgctgcgga ctcactcgtc caaaaaccgt atagcgatga
    43141 gcagcttctc gcacttccat ttcttcagta atttgagaaa gatcgacata cgcatcataa
    43201 ttcggtaatc ctgcacgtct agcgatattg gagacagtaa aaggattgtc tccagaaatc
    43261 actttcagat ccacaccttc ctcatgaaga taagctagtg tttcttgagc gttttttcgg
    43321 attggatcat cgatttcaaa cagagcaacc ggctcaacaa atgctggttt ttcttcgttc
    43381 aattgctgat ttggagcgat ccccaacata agtacgcgat agccattcgc ttgggctgtt
    43441 tttagactgt ccggaagccg atcttcttcc accaaacgtt caggcgctcc tacgtacacg
    43501 actcccagtt ctggaaattc catcgcgccc catttgcgat cagaagaaaa tgcgactttg
    43561 tttgtcacat cataccgatt agaaagctca aaataatctc tcatagcccg catagtgatg
    43621 ttgttatcac tgctttctgc cagatacgtc cccataattt ctgggatggc tttctcgtac
    43681 agcggatgta gtgcttctac ccgctggata ctcattttcc cttcagtaat ggtccctgtt
    43741 ttatccaagc ataatgtatc gacatgggcc agcgtttcaa ttgaatacat gtcttgaaca
    43801 aggattcgtt tttttgccag tttggttacc cctgtcgtta atgcaatact tattagcaat
    43861 accaaaccct ttggaagcat gcctaacaaa gctgctgcag atgcaataac agaaattttg
    43921 atagaagcat cgcggatgaa cagtgcttcc aaaaatagta tgatccccaa aggaataatc
    43981 acgaaactag tgaatttgga tactttgcga atcgaatcta caagttcaga acgaatagct
    44041 ttgtgagtct ttgcctcgct agtcaagcgg acagcataat tatctgcacc tactcggata
    44101 acttcagcca tgacagaacc gcttgtcaaa aagcttccag aaagcaattc agctccagtt
    44161 tctttgacaa tcaaatcaga ttcgcctgtc aaaagagctt catttgcttc tgcccgtcca
    44221 gatataacct gtaaatcggc tggtatctgt tctcctgctc cgatttttac caaatctcct
    44281 aataccagct ctttagtaga aatcgattgt tctttaccgt cccggatcac tagtacttct
    44341 gattgggaaa caatcgataa tttccgtaca agatttcttg catgtatttc ttgataaatt
    44401 ccaataacga tattgacgac aataattgct aaatacgcca tgttggaata cgcacctaca
    44461 aataacagac aaatcccaat accaaagttc agaaaattga ataacgtcaa tatattatcg
    44521 ctgaatattt gttttgtact ttttgatgca tcttcctggt agtcattttg ctggccagaa
    44581 gccattttct gaactacctc ttgctgtgta agtccattca ttttcttttt ctccattttc
    44641 cgccaacttt caaaaataat cacgtgttta agtctagcgt gttccaagat taaaaacaac
    44701 ttgtttttat aaaatttata gaaaaacaca cgaatgattt tacaaaaaag atgccaacct
    44761 ttttgtttga atcacccgtg tgctgttttt attatcaatc tatctttact tatattgttc
    44821 ctccaaacgg ctcatccacc aacctagtgc tgtaccggct ataaaaagaa caggacaagc
    44881 tagtacccca gcaactccca atcccgcgta atcggtagga atgatcataa tcgttgaagc
    44941 aaaaactaca cctaaaatga aatggaatag cttgctatat gcctttgaga aaatgtaatc
    45001 catgattttt gacaatccaa gaacacaaag aatcccacca attccgatcg gcacaatcac
    45061 ttcaagtcgt acttctttaa atccgtcagc catcgcttta tacaatccca tatacaccaa
    45121 aaaattagaa gggctcaatc ctgggacgat catacccaaa ccaatcaatc cgccggcaat
    45181 cagccatgtt ccaaaattct gatcgacttg cccgtcaaat aattgagcgc cgtataataa
    45241 aaacaaataa gccagaacaa aagtaacaac catgatgatc acatcagaag tatctcttcc
    45301 ttttttccct gcttctttcc ataacgcagg aacagttcca gcgatacatc cgacaaaaaa
    45361 ccataatatc gttgtttcat acgcacctaa taaaaaactg actaaaaaag aaagtagaaa
    45421 gatgcctgtc actccgccta gaccaacagg cagaaaaaac aaaacatttt ctttgaaatt
    45481 ttttgtaagg tgggctaaaa aagaaatcag ccgctcatac atgccaaaga cagctgctaa
    45541 tgcaccaccg ctgactcctg gcaagataaa tcctgaaccg ataaacattc ccttcacaaa
    45601 ccgtaacatc cagtctttgg ttgtgtaccc cacttcttga ttttccattt tttgcctttt
    45661 ttccactatt tgcgtactcc ttctttcatt taattttatt catgattgaa catttcctgc
    45721 tattttactt aaagactttt tcataggtct ctattatttt ttcattcatt ttgacaatat
    45781 caggataaaa catgttacct ccattatgat agagatagcc gccattgtaa agcaaggact
    45841 ggatccgcca atatcggtat tgtttccctt cctcatttcc taaaagtggg ctcagcacat
    45901 ctttagaata cgcttctgct aattctactg tgttttctcc gccatgattt tttacatagg
    45961 caatataatc tttcccaaaa ttatatgctt ggacagctgt ccataaatcg catccttgtt
    46021 tctttgcttc tactatcatt tccgccaaat atttgacacc ttgatcaata ctgtcagctg
    46081 gattatcaat gatctcagct tctccatgca gactttccga gctctgcatg agatctgtcc
    46141 cttttccttt tgattcggtc aatattatcg ccttgatcaa attctcgtag ttttccatat
    46201 gataattttt tgcagctgtt gttatttcta tttgatagct ttccactcgt tttaccgaac
    46261 gataagttct tcctcataaa aacaacaccg caccaataac cgtcagaatg atcatgtaaa
    46321 aaagaatacg gataatagat aatcgttttc gcttcatttc atcacctagt tattttgaca
    46381 tcttttttta tcttactatt tctttatctt cttggctatt tcttccttga tttctgtgta
    46441 cgccttaaga aaaaatgaat tttcctgtat tttttgacat cttacgaaaa tgatcgttgt
    46501 ttttcagtaa aaacaattgc ccaatgactg atataatcgt tatactttac acagtaggtg
    46561 attatgtgaa aaaagaaaag aaatatgcaa aaatgccaga tgggagcgaa atttactatg
    46621 aaaagagcgg tcaaggcttc cctctctttt tactgcatgg caatgacggc agcggccgct
    46681 ttttttcaga acaggtccct gtactcgaac gctactatac cgtgtatcta gtagatagcc
    46741 gaggacatgg tcgttcgaca aacgaagcat ctatgttgaa ttttcagtta atggccgagg
    46801 atctgaatac gatcatgttg ctggaaaaaa tcgatcaggc agattttctt ggatttagtg
    46861 atggagcaaa tctagcactg gtatttgcca gcagcttccc taaaaaagtt catcgattga
    46921 ttttaaattc tggaaacacg cttgttaaag gcgttcgttt ttctgccaga gtcatcagta
    46981 atattcatta tgcatgggta tggttactct ctttatttcg cccaagtctt cgcaaaaatc
    47041 ttttagttat caaacttttg ctgcatgaca tcgggcttac agaaaatgat ttaaaaaaaa
    47101 tcaactcccc cactttgatc atcgtgggaa aaaaagatgt aatcaagcta aaacattctt
    47161 tgtatatagc taaaactata ccaaaagctt cctttgtgct tgtgaaagaa caaggacatg
    47221 aattagctag aaaagatcct gaacgcttca atcgagaagt tcttcaattt ctttctgaaa
    47281 cataactatt aggaatagaa ggtgatttat ttgttaaaaa ataccataca atggctgaaa
    47341 aaccacctag gactgttcaa aacaatcttt ttgatttctg tcattgtcat tattgtcggt
    47401 gaattgatgt ccatcggtaa aactctatct atccagcaat taggagaaac gtttgccact
    47461 atacctgtat ggaaaagcgg actaatgcta ttgattggtc ttatctctgt gttacctatg
    47521 gttggttatg atattatttt gaatcggatg cttgaacaaa agcagaatcc tcgttacctg
    47581 tttgagacaa gctggctgat caatacgatc aacaatattg ccggtttcgg cgggtttgtt
    47641 agtgtggggc ttcgctctga actatatgga caaaagaaag agagcagaaa cgtgatccaa
    47701 gcattatcga aagttttctt gtttttgatg gctggacttt ctatttacag tctgctttct
    47761 tttttacttg tagtcttcac gccagttgct ccttttttga agcaatattg gatatggttg
    47821 atcggcggcg gtctatattt tcctgtcgtc tttttattta ctactttcaa aaagaaagga
    47881 ttccttggtg gtacctcact taaaactcaa ggacagctat tgctagtttc attgttggaa
    47941 tggaccggtg tcctgtttag tttcctttct gttggttatt taatggaaat ccggattgat
    48001 ctgtggcaaa caattccatt attcattgct gcttcagtga taggaatcgt gtctatgatt
    48061 ccaggagaaa ttgggagttt cgatgtaatg atgattatcg ggttgtccgc tattggtgtg
    48121 ccaagagaaa tagtcgttgt gtggatcttg ctctatcgcc tgttctacta catcattccg
    48181 tttttgattg gaatcgtctt tttcttcaaa aatatcggtt ccacttttga tcagcgttat
    48241 tctggtatcc ctaaacaatt agcaacagaa attgcgcata aaatcgtggt tgtcttatta
    48301 tacttttctg gaatcatgct agtactttct gccactatcc cgcaagcttt tacagagttt
    48361 cgctggcttc atagtttgaa cccattgaaa tttcatttta ttatccaatt tcctagtata
    48421 ctattgggat ttcttttaat cattatgggc agagggatcg cagcacgtgt gaaacgagca
    48481 tatttgccta caatcttctt gattgttcta gctttacttt atgttttatt aagtgatttt
    48541 agtttcacac cggtggtttt cttgtctatt ctgcttctga tcattcttgc ttctaaaaac
    48601 gaattatttc gggaacaatt gatctattca tgggagtggc gtacgatcga tggaatcatt
    48661 attggtgcac tgtccttgct ttacatcgtt attggggtat acaatctgcc ggatttccct
    48721 catcggcgtc accattttat ttcctttttc ttatttcctt ctgaaaagat ttggttttcg
    48781 ggattgttgg ccatcatcgc tgttagtttc atgatcgttc tatttgttca tttccttcaa
    48841 ggagaaaaga aacagattgg cgaagccttt aatgaagaaa aggccctgaa gatccttaca
    48901 acttatggag gaaactccga tagtcaattg atcttcttaa aagataagcg gatgtttgcc
    48961 tatgaaaaag acggtgaacc aactgttctt ctccaattcg cttgcttcaa taataaatgt
    49021 attgtaatgg gtgatccttc tggtaagaaa gaagattttc cagaagcaat cgaagctttt
    49081 atcgaagaaa cagatcgact atgctattta ccagttttct acgaaacaag cgaagagatt
    49141 gttatgattt tgcatgaatt cggctacgat ttcattaaga tgggggaaga agcttatgta
    49201 gacctgaata gttttacaac ttcagggaaa aaaatgaagg gcactcgtgc tgtcttgaat
    49261 cggatcgaac gagaaggatt tacatttgat gtcttacaac caccattttc tactgagcaa
    49321 atgagtactt tcaaaaatat ctctgataat tggttgggtt cacgaaagga aaaagggttt
    49381 tctttaggct ttttctctga agattatcta caaagagcgc ctattgcagt tgtcaaaaat
    49441 acccaagaag aaattgtcgc ttttgcaacg atcatgccta cttacacgaa caataaagta
    49501 ggaacgattg atctgatgcg atatgattct aaaaaagcac cttctggcag tatggatttc
    49561 ttgttcttgc atttgtttgc ctatatgaaa gaagaacata tccaatggtt caatttaggg
    49621 atggcccctc tttcaaatgt cggaacttcc agaaagagtt ttttacagga aaggattgca
    49681 tacttggttt acgagtttgg ttctcatttt tattctttcc atggattaag agaatataaa
    49741 agcaagtacg ctacaaactg ggtctcacgt tatactctat attctagaga tagttggatt
    49801 gcatatgtta tgattgcttt gctgattatt gataatgcgc cagttgagca gacacaaggt
    49861 agaataagtg gtttgaaaaa atggatacga agaaagtatt aaaaactgca taccgataga
    49921 tgaacactct catgatgaat aaaagagact gaagtaaaac gaaagttcat gcttctacaa
    49981 tctcttctta tacaattgaa gaacaaagag cggtccaaac agaaatcctg tttggaccgc
    50041 tcttttattt taaacatttt tcattaccaa aatattcgag tcgcgtttct tatgcttcga
    50101 tctctgtaac gatacctgaa cctactgtcc gtccgccttc acgaattgag aatgtcgttc
    50161 cgttttcaac agcgattgga tggatcaagt caacatcaat cgtcacgttg tcaccaggca
    50221 tgaccatttc tgtatcttct ggcaatgtga ttgttcctgt aacatcagtt gtgcggaagt
    50281 agaactgtgg acgatagttg ttgaagaatg gagtatgacg tccaccttct tcttttgtca
    50341 acacgtacac ttctgctttg aattttgtat gtggagtaat ggaacctggt ttagcaagta
    50401 cttgtccacg ttcgatatcg tcacgttgga tcccccgtaa caacacgcct acgttatccc
    50461 cagcttcccc gtaatctaac gttttacgga acatttctac acctgttaca actgcttttt
    50521 gtgtttcagg tttgatccct acgatctcta cctcatcacc gacacgaaca gcaccacgat
    50581 caatacgacc tgaggcaaca gttcctcgac ctgtaattga gaagacgtct tccactggca
    50641 atagtaatgg tttatctgta tcacgttctg gtgttgggat atattcatct actgtatcca
    50701 tcaattccat aatagcagct tcagcatctg gatcgccttg caatgctttt aatgcggaac
    50761 ctttgatcac aggagtgtca tcgcctggga aaccatattc gctcaataac tcgcgaactt
    50821 ccatttctac caaatcgatc aattcttcat catcgactaa atcaactttg ttcaagaaaa
    50881 caatcaggta ttttacacca acttggcgtg acaataaaat gtgttcacgt gtttgaggca
    50941 tcggaccgtc agttgcagaa acaactaaga ttgcaccatc catttgtgct gcccctgtaa
    51001 tcatgttttt cacatagtcc gcgtgtcctg gtgcatcaat atgcgcgtag tggcgttttt
    51061 ctgtttcata ttctacgtgt gcggtattga tcgtaatccc acgttcacgt tcttctggag
    51121 ctgcatcaat actagcatag tcttgaggat ttgctaggcc ttttttgcct aatactgtag
    51181 tgatcgcagc agttaaagta gttttcccat ggtcaacgtg tccaatcgta ccaatgttaa
    51241 cgtgtggttt acttctgtca taatgttctt ttgccataat ttacaaacct cctaaataat
    51301 atagatcact gaaaaatttc agctctcacc tttttattat agtctttttt tgactaaatc
    51361 aaaaagaata cgtccgactt actttttgta agattcgaac taagcttatt atacgtcaat
    51421 attggtcaaa gtcaactaag agcactcgtc tgtcttatac gcgaaaaaag taccttttat
    51481 aaataaaaaa atgtgttttt tgagaaaaac ttcaagatag tccttaactt tcttttgaag
    51541 caataagaac aaaatgagga aaagtttaaa attcagctgt tgccaacaac tttcaataga
    51601 cgaaatcttc tatttcgtta ataataagga aagaggtgaa gtacatgaaa aaattaattg
    51661 gcgtacttgt cgttttaggc atatttctcg gcggaagttt tgcagcttat cattattttt
    51721 acggcggcga agcttattat acaaaaatca cgacgaatgg cgaaatgtca gctacccaag
    51781 cagataacgg agagaaattc acgacttata cctacaagca agcggcttat aataaagatg
    51841 gtgaaaaaaa acaggtgacg cttcgcgaag aaagagaaca tccattaaag atgaatgcgt
    51901 atcttaaact caaagtcaat cctcgtaaag gtgtattaag ctgggaagaa ataaaggctt
    51961 ctgaagtgcc taaaaatgcg gcagaaaaaa tcgataatta gtaaatggtc ttaaaatttt
    52021 attagataaa aagaggctgg gacagaagcg tttaactcca agaaataaga aggagttgct
    52081 tctgcttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt tacttttgtc
    52141 tcacgctctt tttttgttat attcgtcttt tccgatcctt ctcttgacac aattcattta
    52201 actgatcact ggtacttgaa cacctaatat tctagtcatt tttataaacg attcaatact
    52261 tcatccacat ttttgatcat cacggatatc gtaccatccg gattattcac aaattcaatc
    52321 aaatctgggt tgcgatatac atctactggt acgatcagtt cgatcccgtt tgatagcttg
    52381 aatttttgtt ttccaaattt tttctcagaa atttcttgta cttcttttac aggaggtgct
    52441 tctgggacaa atccttgctc ttgtacttct tctttaaaag ccatttttgc cgtgatgttt
    52501 tccttgaaga ctttttccgc aatcatctca tgatccaacc gacctttttc ttctatcgta
    52561 tcatagacag cttctttcaa atcagccatg atatcaaatt cttctgtctc aaattttttt
    52621 ccaagatgct tggctacttt ttggatcact tttacatttt cttctaaaga aggtgcagga
    52681 acactttcga tcactcttcc agaaaaataa agctcttttt tgccagaaaa ctcatatttt
    52741 ttttcaatta attcgtaggt ccaatcagtc aaattcacag cgaatgcttc atctgctttt
    52801 tgtgttttcg cccctagaat tgctcgattc aaaatcaagc ggttgtcgat ccctgcttcg
    52861 tctgcttcaa caaagtgagt gtaagcctca cgataattga ctttcaataa agataagtac
    52921 atcactgtat ctaattcata caagacaaca aatacatctg cactaggtgc ttcttcactt
    52981 tcttggtaga tatcgaacca ccgctgaacg attgcttcac ttgcttccac aaaattttct
    53041 tgagcgattt taagtgattc aacgatcatg ctttcttctg ctagttgtcc agtttttgtt
    53101 tgagcagaag agattttttg gatcttcttt tgaagaaaat cacgaatata ttcttttgtc
    53161 agatccagct ctttttgtga aaagactgga gaaccagttt ctctgtcaat aatatgcaga
    53221 attgcttttt ttaaatagat atccattttc tcattccctt ctgtcttagt ttacggaaaa
    53281 aaaatcaaaa ataccagaat caaaatcatt ttcttttaaa caaaaatttc aagagtagaa
    53341 acaaaatgga ttcttttttt cgaaaaatgc gctacactta tagttgaacg aataaaaaag
    53401 gagctgaaag tatgaattca ttaacaagca cttatcgtct atcaaatggt tacgaaatcc
    53461 cggtagtagg attcggcaca tggcaaacac ctgatgggga tgtcgctgtt tcctctgtta
    53521 aagaagcgct ggctgcaggt tatcgccata tcgatactgc ccaaggatac aagaatgaag
    53581 aaagcgtcgg ccaagcgatt aaggaaagtg gaattcctag agaagagata ttcttgacaa
    53641 caaaattatg gaatgcaaac cacagctatg aacttgtgat gtcttctttt gaagaatcat
    53701 tgaagaagct ccaaacagat tatttagatc tgtttttgat ccactggcca aaccctgttg
    53761 cttttcgtga taattgggaa caagcaaatg cagatacatg gcgagcattt gaagaactgt
    53821 atgaagcagg taaaatcaaa gcaatcggtg tgagcaattt cttaccccac catctagata
    53881 cactagcaaa aacagcaaaa attatgccga tggtcaatca agttttcctt gcaccaggcg
    53941 aattacagac aactgtagtt gagtatgcaa aaaaacatga tatgatcctc gaagcatata
    54001 gtccattagg cactggtaaa atcttcgatg tgcctgaaat gaaacaaatc gctgaagcac
    54061 atgacaagac gattgcacaa gttgctttgc gttggtcgct gcaacacgaa ttccttcctc
    54121 ttcctaagtc agtgacacca agcagaatca aagaaaatac tgaattattt gattttgaat
    54181 taacagaaga agaaatgaaa caaatcgatc agttagatgg tgtagtcggc aaagcaaaag
    54241 acccagatac tacccaattt taagtcgaaa ggtgatttta tggatccaat cgaacgcttg
    54301 aattctctct cggaagaagt tacccagact tttcattccg attttgtctt tttgatcgat
    54361 gcagagaaaa tccaacattt tcctgcaaga aactggacac atgatcaaat catcgaagaa
    54421 ctgaaaaaac ggttcgatca ttcgctaatg gtcaaaccct ggcacgaaca tgaagtcatt
    54481 tattcacctg aactacctgt ttttgccttg ataccgaaaa aatagaaaga atggaacgga
    54541 ttatgtatat atacataatc cgttccattt tttatttttc tttcaaaaaa cgggaaaata
    54601 cgcaaatttt tctgttgtaa tttgcgtata cgcaagtttt tttgcattac tccttttcat
    54661 ttggctgttt ttttcttata atcgaaaggg tatgagcgaa ggagagaaca tatgaagaaa
    54721 aaacagaaac aaaaattatt aaatcaattc cgtccttcat tggatggtgt aagaggacaa
    54781 ttattccgtt ctctagaaga agagagcttg cgacgttatg gtttgccgtt acaagtcatg
    54841 cttgatccca aaaaccgtca aatcttaacg atcggattag ctgggaaaac aaatgaagat
    54901 acacggatca acgtgccaat cgatgacaac ttcaatactg tattaaagag aattcgttca
    54961 ggagaaaacg ggattttcga acgattccgt gacaatcttc ttattgaaat cgtgtcttac
    55021 tggaatgatc aacgattgaa aaacgatgaa ccaactgcgc aagctgttgc gacaccagtt
    55081 acagaagaaa cagtcagtgt gccagaagaa aaaacggaaa aaccaaaagc ggacacaact
    55141 gaaaatacac gctcaactga tacgaatgcc gctttaagtt ttgctgattt ttcaaaagcc
    55201 gttgctgagt atcctaaatt ttacacagaa caaaatgaac atgaagtagt tattaatgaa
    55261 aaaaatgctg atgaacctcg taaattagca actatttcta tgactactga aaatgagttc
    55321 gttatcgaaa aagcactcga acggaaatac aaagtcaaat tgactttgat tcctttgatc
    55381 gaacaatttg cagcgacagc gattactgat cgataaaaat tctatttgtc ataaaaggct
    55441 gggatttaaa atcccagcct ttttagtcat ataataattt ctacagcaag taaaaagaaa
    55501 ttccttctaa catcaggctc aaatatcagc ttgacctg
//
LOCUS       DANKKG010000005        54866 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000005 DANKKG010000000
VERSION     DANKKG010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.005"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(<1..235)
                     /locus_tag="PW004_000226"
     CDS             complement(<1..235)
                     /locus_tag="PW004_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2725227.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYL"
     gene            429..722
                     /locus_tag="PW004_000227"
     CDS             429..722
                     /locus_tag="PW004_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725228.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            766..1077
                     /locus_tag="PW004_000228"
     CDS             766..1077
                     /locus_tag="PW004_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725229.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            1074..1698
                     /locus_tag="PW004_000229"
                     /pseudo
     CDS             1074..1698
                     /locus_tag="PW004_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            1839..1997
                     /locus_tag="PW004_000230"
     CDS             1839..1997
                     /locus_tag="PW004_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725230.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            1998..2360
                     /locus_tag="PW004_000231"
     CDS             1998..2360
                     /locus_tag="PW004_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725231.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            2397..3245
                     /locus_tag="PW004_000232"
     CDS             2397..3245
                     /locus_tag="PW004_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HDL2725232.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            3235..4710
                     /locus_tag="PW004_000233"
     CDS             3235..4710
                     /locus_tag="PW004_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HDL2725233.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            4976..5383
                     /locus_tag="PW004_000234"
     CDS             4976..5383
                     /locus_tag="PW004_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HDL2725234.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            5386..5601
                     /locus_tag="PW004_000235"
     CDS             5386..5601
                     /locus_tag="PW004_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725235.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            5605..5985
                     /locus_tag="PW004_000236"
     CDS             5605..5985
                     /locus_tag="PW004_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDL2725236.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            6119..6274
                     /locus_tag="PW004_000237"
     CDS             6119..6274
                     /locus_tag="PW004_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HDL2725237.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            6343..6816
                     /locus_tag="PW004_000238"
     CDS             6343..6816
                     /locus_tag="PW004_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HDL2725238.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            6813..8507
                     /locus_tag="PW004_000239"
     CDS             6813..8507
                     /locus_tag="PW004_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDL2725239.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            8473..8658
                     /locus_tag="PW004_000240"
     CDS             8473..8658
                     /locus_tag="PW004_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725240.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            8662..9837
                     /locus_tag="PW004_000241"
     CDS             8662..9837
                     /locus_tag="PW004_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDL2725241.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            9830..11353
                     /locus_tag="PW004_000242"
     CDS             9830..11353
                     /locus_tag="PW004_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDL2725242.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            11409..11693
                     /locus_tag="PW004_000243"
     CDS             11409..11693
                     /locus_tag="PW004_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HDL2725243.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            11680..12015
                     /locus_tag="PW004_000244"
     CDS             11680..12015
                     /locus_tag="PW004_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HDL2725244.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            12310..12936
                     /locus_tag="PW004_000245"
     CDS             12310..12936
                     /locus_tag="PW004_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725245.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            13678..14514
                     /locus_tag="PW004_000246"
     CDS             13678..14514
                     /locus_tag="PW004_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDL2725246.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            14578..15009
                     /locus_tag="PW004_000247"
     CDS             14578..15009
                     /locus_tag="PW004_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2725247.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(15071..16393)
                     /locus_tag="PW004_000248"
     CDS             complement(15071..16393)
                     /locus_tag="PW004_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HDL2725248.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(16547..18187)
                     /locus_tag="PW004_000249"
     CDS             complement(16547..18187)
                     /locus_tag="PW004_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HDL2725249.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            18520..18990
                     /locus_tag="PW004_000250"
     CDS             18520..18990
                     /locus_tag="PW004_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HDL2725250.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            19212..20315
                     /locus_tag="PW004_000251"
     CDS             19212..20315
                     /locus_tag="PW004_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDL2725251.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLKDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            20599..21279
                     /locus_tag="PW004_000252"
     CDS             20599..21279
                     /locus_tag="PW004_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HDL2725252.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            21369..22403
                     /locus_tag="PW004_000253"
     CDS             21369..22403
                     /locus_tag="PW004_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HDL2725253.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            22417..22752
                     /locus_tag="PW004_000254"
     CDS             22417..22752
                     /locus_tag="PW004_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725254.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            22736..25216
                     /locus_tag="PW004_000255"
     CDS             22736..25216
                     /locus_tag="PW004_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725255.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWKLTAGPTS"
     gene            25272..26831
                     /locus_tag="PW004_000256"
     CDS             25272..26831
                     /locus_tag="PW004_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2725256.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            26828..28192
                     /locus_tag="PW004_000257"
     CDS             26828..28192
                     /locus_tag="PW004_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL2725257.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            28363..29433
                     /locus_tag="PW004_000258"
     CDS             28363..29433
                     /locus_tag="PW004_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2725258.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            29448..30116
                     /locus_tag="PW004_000259"
     CDS             29448..30116
                     /locus_tag="PW004_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2725259.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            30136..30714
                     /locus_tag="PW004_000260"
     CDS             30136..30714
                     /locus_tag="PW004_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2725260.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            31115..32026
                     /locus_tag="PW004_000261"
     CDS             31115..32026
                     /locus_tag="PW004_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDL2725261.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(32114..33031)
                     /locus_tag="PW004_000262"
     CDS             complement(32114..33031)
                     /locus_tag="PW004_000262"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HDL2725262.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            33233..33670
                     /gene="zapA"
                     /locus_tag="PW004_000263"
     CDS             33233..33670
                     /gene="zapA"
                     /locus_tag="PW004_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDL2725263.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            33729..34277
                     /locus_tag="PW004_000264"
     CDS             33729..34277
                     /locus_tag="PW004_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HDL2725264.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            34392..36752
                     /locus_tag="PW004_000265"
     CDS             34392..36752
                     /locus_tag="PW004_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HDL2725265.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEEQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLYDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            36839..37153
                     /gene="trxA"
                     /locus_tag="PW004_000266"
     CDS             36839..37153
                     /gene="trxA"
                     /locus_tag="PW004_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDL2725266.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            37335..39113
                     /gene="uvrC"
                     /locus_tag="PW004_000267"
     CDS             37335..39113
                     /gene="uvrC"
                     /locus_tag="PW004_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HDL2725267.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            39258..40157
                     /locus_tag="PW004_000268"
     CDS             39258..40157
                     /locus_tag="PW004_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2725268.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            40150..41412
                     /locus_tag="PW004_000269"
     CDS             40150..41412
                     /locus_tag="PW004_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2725269.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            41573..43471
                     /locus_tag="PW004_000270"
     CDS             41573..43471
                     /locus_tag="PW004_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HDL2725270.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(43801..44520)
                     /locus_tag="PW004_000271"
     CDS             complement(43801..44520)
                     /locus_tag="PW004_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HDL2725271.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            44857..45189
                     /locus_tag="PW004_000272"
     CDS             44857..45189
                     /locus_tag="PW004_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725272.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            45176..47179
                     /locus_tag="PW004_000273"
     CDS             45176..47179
                     /locus_tag="PW004_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HDL2725273.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            47198..47668
                     /locus_tag="PW004_000274"
     CDS             47198..47668
                     /locus_tag="PW004_000274"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HDL2725274.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            47689..48273
                     /locus_tag="PW004_000275"
     CDS             47689..48273
                     /locus_tag="PW004_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HDL2725275.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            48288..49274
                     /locus_tag="PW004_000276"
     CDS             48288..49274
                     /locus_tag="PW004_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HDL2725276.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            49264..49575
                     /locus_tag="PW004_000277"
     CDS             49264..49575
                     /locus_tag="PW004_000277"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HDL2725277.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            49597..51378
                     /locus_tag="PW004_000278"
     CDS             49597..51378
                     /locus_tag="PW004_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HDL2725278.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            51371..52747
                     /locus_tag="PW004_000279"
     CDS             51371..52747
                     /locus_tag="PW004_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HDL2725279.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            52754..53389
                     /locus_tag="PW004_000280"
     CDS             52754..53389
                     /locus_tag="PW004_000280"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HDL2725280.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            53419..54774
                     /locus_tag="PW004_000281"
     CDS             53419..54774
                     /locus_tag="PW004_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HDL2725281.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
BASE COUNT        18387 a         9315 c        11627 g        15537 t
ORIGIN      
        1 atagataggt agtggataca tctaatacct ttgaaagttg tttaagcctg gtgttattgg
       61 gaagattata gccattttcc caattactaa cggcaccttt actggccggt ggggataatt
      121 tacctccaag ttgttccatt gagagtccct tttcaattct agccagtcta attctttgtc
      181 ctgtctccac tgcttcgggg tatttttttc tagccacatt ttcaccacca ttcatatatt
      241 ttctctacta tatcacgatt taaatatgtg tgaaacataa aagttcaaaa aagtatgtta
      301 aaattcttgc aaatgaaaag gtatgatggt atattgagtt tataagaggt atagaaatgt
      361 attcaagagc ctcttgcatt tttttaaaat ctttgtatag aaatgtatgc taacgagggg
      421 ggataaatat ggaattaaaa attgacaaac tcacgcttga tgatccagct tttaaaatta
      481 ttgacgatcg agttgttcaa caagtagagc aaactcaagg aacgttactt tcattattag
      541 aaaaaaagta ccagtttcca aaatatatga acaaggcaca agctgctaag tatatgaacg
      601 tttcatacaa tactatgatg cagaaatatg ttcctaacgg tttaaagttg attatagttg
      661 atggagtcgt aagaatttca caagatgaat gtgatcgctt tatggaagaa taccaaaaat
      721 aaacgcaagc cgtgggggca aagggattaa taggaggaaa taataatgga atcgacacaa
      781 aataaagcaa tcgaaaaagt attatcaaca attcttacag aagacacagc ggcagaatta
      841 gcaaacttgg aaggcaaagc gctagaagaa acgtttgaat gtttgtacga acaaatggac
      901 tatcaaaaat tgctaccaca agaaccaacg gtaagcggag tattgcgtgg cttgaacgat
      961 ttagtacaag cagagtttaa agaacgactt tctattgaag agtatcaaga aattttgtat
     1021 cagcaagttg agttgttagc gagcttgcta gggcttgaac tggaggacgt tgaatgaaaa
     1081 ctgaaatttg gaacggacac attataagat ttgtcgatat taatgatgaa tggtgggcag
     1141 tggcgaaaga tgttgcggaa gcgttaggtc ttaaacaggt tactagagcc attcactctt
     1201 tgcctaaaga tggggttact actagtaagg tcatcgattc attgggcaga acacaagatg
     1261 taaacatcat caacgaaaaa aaatatttac cgcatggcat tcaaaagccg taaaaaagaa
     1321 gccgaggcat ttcaagactg gattttcgac atcatcaaag aactacgcca gtccacagga
     1381 cttgaaggct ttcaagtatt ccgcatgcta gacaaagaac accaaaaaga agcaatgact
     1441 aaattaagcc acgctattac tgaaccaaaa ccagtagact acattaaagc gaatgtgata
     1501 gccaataaag ctgtatcaac tatttatggt cattccaaaa tggtaaaaaa gaaagatatg
     1561 actcctgaaa tgttggttga tcgtgaacct attttagatg agacagtaga attaatgact
     1621 gttaaagaaa aatatgggtt gcagttttca gtgagtgaga aaatttataa tcgttctgct
     1681 gaattgcaga caacttagga gcattaacat gatcgagaac aaagaaaaag ccttatccat
     1741 aacagtcttg gcggacagga taaaggcaac ttaattggga taaaacccga tattctttgt
     1801 tccgattata ccatattaaa aaatcggagg gaaccattat gaaacgaaac ttaattatag
     1861 gaagcatttt aatcggcgga attttcggct tattaccatt aattgcaaaa atagctatct
     1921 ttgcaggatt agcgttatat ctcttcactg cttatgacga gtacgactat caattaagaa
     1981 ctggagggca taagtagatg tacaaaacaa aactattaaa ccaactcgat agccttgaat
     2041 tagaggaaat taatcaaggg atcgctgaac tagaaaataa tatcgggaaa acctactttg
     2101 gaaattcatt caatgaaaaa ctaacagttt tgtatgtact caaaaaacat gcagaacaca
     2161 aaataatttg tcgagaaatc aacgagctaa aaaatcagat tttaaccgca tggttaaaca
     2221 ttactgatat gcaagaagca agggtgaaaa catttaatac atgggtaaag tatcagaatc
     2281 aattaaaagg tgctgaattt gttcgggatg ggttgaaata cgaacttgag cagttgaagc
     2341 tcatggaggt gtctgaatga tttataccag tattttgaaa aaggaggaga ggtttattgg
     2401 caaataatta tcaagcagca acggaattac aaaagtcggg atggtcaatt tatcccttat
     2461 ctcctggaac taaaattcca ataaaaggct ctagtggttg gaatgatgca actaacgatt
     2521 ctaatcaaat taatgaatgg tggacggaag atgcaactag aaatattggg ttaatgcttg
     2581 aagatcatca aatggtggta gttgacctag atcaacattc tgaaacacga aacggcgttg
     2641 agaattataa aaaacttgct tcaatgtatg atccatttcc ttctacttat acggaaatca
     2701 caccaggtaa aggaatccat ttcttcttta aaattcctga tggtgtaatt atccaacaag
     2761 aaacaggtgc atttgctgat atcttcgggc gtgatgataa aggtaaaagt ttaagcggga
     2821 ttgatattat cactaaagga gttccaatag cgccaactat gattatttca agaagtgttg
     2881 ggagagcata tgaacctatt gaaaacgtat ctctaaataa tattgcacca gctcccgatt
     2941 ggctagtgaa cctactgcta aaaaaaccgc aagaaataaa gactcaatac cgacccgtag
     3001 caaaaacagg gacagcaaaa aaattagata tgattgttca aggcgctggc gaaggtagta
     3061 gaaacgatca tttaactaag ttatgcggtt ggttactatg gcacggtgta gataatcaaa
     3121 ctttagcaga attgatttat acagcaaatg cgtacaatga accaccgcta aaagataaag
     3181 aagtgaatca aattattcga tcgatgatta aaaaagattt gagggggaaa gctcatggct
     3241 cataagcata aattagatag tattttagac tttcccgaag ccagcgagcg tgaagacaat
     3301 atcattgaat tgaaaacctg gatgtcacga ttaagatgca ataaagacga tcagattaaa
     3361 agtaacagtg ttgtgaatgc agaattgatt ttaaccaatg atagcaattt agctggaaca
     3421 attgcttata acgaatttag cggatacatt cacctattaa aagattctcc ttggatcaat
     3481 cgtaccgctg gagaatggga agacagtttt gaggatgcct taacggcata tattgaagaa
     3541 aactacaatg tagtttttga tgacaacaaa atacacaaag cagtagtaaa tgtggctaga
     3601 aagaatgttt tcaacccagt aaaagaacgt atcgaaaaag ttaaatggga tcaacagcct
     3661 agactggaaa cactgtttat tgacctttta ggggtagacg acaaccttta cacacgagaa
     3721 gtaacaaaac gttggatagt cggatcagtc gcacgtatct ataaaccagg aataaagttt
     3781 gagatcgttc cagttttgga tggaccgcaa gggattggta agagtaccgt tccagcatta
     3841 ctttacacgg atgatttttt caccgattca ttagattcgc taggtgaaaa gaaagatgat
     3901 tatatgcagc ttcaaggcaa tgtaattatt gaacttggag aattatcttc aatgaacaaa
     3961 actaaaattg aacagataaa aaacttcatc agtgccaaga tcgataaaat tagacctcct
     4021 tatggtcgga atgtcatagc ttgggcaagg caatgtgtat ttattggaac gtcgaatgat
     4081 gggcagtatt tgaaagatga tacaggaaac cgccgttttt atcctttgcc ttgtaagaat
     4141 aagccaaaga tgaatccgtt taaaacaaag gatgaatatt ttcttcaagt gttagctgaa
     4201 gcaaaggtgc tttttgataa aggacaacga atctatttta gtcccgatga agataaagaa
     4261 gtcttagaaa ttgcaaaaga ctatcaagag gatgccaaga tggagaatcc aattaaagaa
     4321 gcaatcacta aatatttgga aatggaaatc ccgtataaat gggaagaagc tccagcgtgg
     4381 gcgagacgtt cttattatca gaactatcct gacagtaaat gtaatgagaa aaatttacag
     4441 catttctata atggagctac tatgcaacaa gagttttatt tagttgatag cgtactcacc
     4501 gctgatattt tggaagctgt ttttgataaa caagcaaagg atttattgaa tggtcgatca
     4561 gatgcagaaa ctaaaaagat tgccttgatt attagcagta ttcctggttg ggaacggaag
     4621 caactgccaa aaagaaataa acgacgagga ttttacaata agtcaaatgc ctatgaaaat
     4681 aaaaatcgag ctggaaagta caaaaagtaa cgggtagtaa cgggtcagta acacctagcc
     4741 cgttactagt ttacccttac tgccccaagg gatacagcaa tttggtaacg gggtaacacc
     4801 ttatttcctt aaaacttttg aaatagttag ctaatatgct ggggaataat gttactggat
     4861 tttacccgtt acctgttacc aaaacgctag aacccttgtc cccgttggga ttgagtggta
     4921 acgggtacct gttacgtagg tgttaccacg tgttacgaat caaggaggaa agtaaatgct
     4981 caatattatt tcaacaaaca aagcaccgaa ctttcaatat acagatgaaa tggatcggtt
     5041 tctaatgaac acgctggcat ttagtgtggg attggtaaca gaagattatt ccacatttga
     5101 tccagaagta ttaaagataa tggaagaaga acctgattgg ctgcaggagt cagtagcgtg
     5161 gtgtcaatcg ctagtagtgg gatcattggt agatagtggc aactatgatg atacaggtga
     5221 gctaatggat gaatttaatt gtctgcttaa cctatatgat cgagcgagac aacgagagct
     5281 tacatcgaat gaggataatt tgtttttaaa catccatgat aagttcttgg ctttgctact
     5341 aacagatgat gaattgataa ctaatttatt ggaggtagaa taattatgca tatggaaagt
     5401 attagatact tcgatggtga gaaacacatt gagtattcaa acactcaaga agatgtagac
     5461 tttattagtt ttgaggaaga taaaatcact aaagttaatt ttaaagacgg aacgtgcttg
     5521 aagatagttt cgccttatat tgaatacaaa tcaaaatggg aacaagatga ttctgacgaa
     5581 cgagatcgct ttggttggta atctatggca gttaaaaaac aatgtaatca tgctggatgt
     5641 aaaacattga ttgattatag gcagaagtat tgtgggaagc ataaagcaaa gcaaacggca
     5701 attaagcgtg aagaaagaaa gcaaagcgaa ggtaaatatt ttcaattcta tcaaagtaga
     5761 acatggcgaa aggcttcata cttgtatcgg ttaaatcatc cagtatgtga ggattgccta
     5821 gaagaaggct tgataaggaa agctgatgtt gtagatcaca aaattgaact aaaagatgat
     5881 tggaatagac ggttagatga aactaatttt cgttctttat gccatgcaca ccataattcc
     5941 aagacagcca atgaaagaca aagacgagaa aagagtaccc tttgagttaa gggggcatag
     6001 tgtgaaactt actgacaatc gatgcctact cattttggta caaataaccg ttgtgaaaag
     6061 gcataagggt aattacaaat aacgattaca cttgtaatca caagtgaaaa agtatataat
     6121 gaaagtagga gatttattga aaaatacaat tgttattact gataaaacag ggaaacagcg
     6181 caccattgag ttacctcatt ttggtcaagt gaccattcaa atgcagaacg gaaaaatcat
     6241 ttatatagat aaattagata aagaaaaatt ctgatcgaaa aacgaaggaa tgtgagctta
     6301 attgcttacg ttcctttttc ttttgtctga aaggaggaca acatgggaca ccctaaaata
     6361 ttacacgata caaaaggcaa tatatcaagt gaagaaaaag caatccgtgt ggacgctaga
     6421 gaagaattgt tcaagcagca accattaata aatatcacgc cccctgactg gatggcagcg
     6481 agcgctagaa gtgaatggaa tcgtatcgta ccaacattaa aaaaagatta tccattgagt
     6541 gaagcagatt atggttcatt ggtagcctat tgtttagcct ttgctcgaat gaaaacagcc
     6601 gaagccgaga taagaaaatc aggtacgttt atcacatgcg aaaacggagt aaagaaagcc
     6661 aatccagcgg ttcgggttca atcacaagct atgagtgatt tgaaaaaaca agccacctca
     6721 ctaggtatga ccttagaatc acgatcaaaa ctagctttga acaaggttaa aaatgatgaa
     6781 cccgaagacc cgttcaaaga gttgatgaat tcatgaatga ttacattgag aaagtcctat
     6841 caggtaagct gattgcaccc aagaaaatta tccaagcgtg tgagcgtcat ataagcgatt
     6901 tggagcgttc taagtcagat agcttccctt atgtgttcga tgaagaacaa gccaccaaag
     6961 cgattaaatt cattgagttg ctaccatcta cggacggtaa agcaatcaag atgttaggat
     7021 ttcaaaaatt cattctaggc agtctttatg gctggcgtac taaagaaggg aattacaggc
     7081 gattcaatcg agcgtttacc agtatgagcc gtaagaatgg gaaaacgtat atcgcaagtg
     7141 gcatggctgc caatgcgttg attatggaac aagaaccagc agaagcaagg caagtattgt
     7201 ttgtaagtaa cgctttgaaa caagctaaat tgggctatga tatgctgtct aattcactta
     7261 gaaacgtggt caagtctagt aagtttttaa gaccacaact gaaaattatg aactctaaga
     7321 ttcagcactt gccctctaat tcgttcgcta tggcactggc tagtgaaacc agcacgctag
     7381 atgggtttgc accaacaacc gcaattcttg acgagtggca cgaagcaaaa actcgtaaaa
     7441 cgtacaacgt cattaagtca ggaatgaccc aacaaaagaa cggcttattg tgtgttatta
     7501 gtaccgctgg gcttgattta aacgttccta tgtacgagga atacttattg ttagatcgtg
     7561 tgctaaaagg cgaagaacaa gccgacaggt actttatagc gatatgggaa ttggacgatc
     7621 ccgaagaaat tcacgatcaa gagaaatgga tcaaagccaa tccgattttt gaaagtgaag
     7681 aaatcaaaaa agtaatgatt ccaaccattc aagatgatgt gaaccttgct ttgaaacaaa
     7741 ataaccttaa ttctgtattg gtgaaaaact tcaatttatg gagacaagcg agtgaggaca
     7801 gctatatgat tgctgaagac tggcaggcaa ccgaagtaga accacaagat attacaggca
     7861 agcccgttta tatcggagtg gatttatcta aaacagatga tttaactagc gtttcatgga
     7921 tcgtaccgct agataacggc gaactttatt gtgattcaca tagctttgta gccaccaaat
     7981 atgggcttca agacaaagaa aagcgcgacg gtttgcctta tcgagaacta gaaaaagctg
     8041 gtgagtgttc cattactcaa ttagaaagtg gaatcgtgga ctatgaccaa gtatttcagt
     8101 ttattcaaga tttgattcaa gaaaatgatt tggaatgtat ggggatttgt tatgacccgt
     8161 ataacgctaa ttcgcttatc agtaaagctg aaaaagccaa ctacccaatg ttagaagtaa
     8221 gacaaggaac gattactcta aacgttccga cccgaacttt tagagaacaa gtttatgaag
     8281 gcaacgttat tcacaataaa aatacgattc tcacccatgc agtgaacaac gctattttaa
     8341 aaacggataa caacggcatt cagattaata aatcaaagaa cagtaacaaa attgatccaa
     8401 tagctgcatt aatcaatgcc tatgtgtttg caatggatta cttcaccaca acggaaggag
     8461 cgaaagcaga caatgaattt tatacaagtg aagaattttc tttctaatta cattcatacc
     8521 gttcttttac ttcttggatt ggtgtgtgtg ttggttgcta tcaccttact aacaaatgtc
     8581 tactatggct tgttagcgct gggcatagtg cttattgggg tagcggtcat gctaaataca
     8641 gaacagaagg gaggttaaaa gatggcattt tttaaagcga gacaaaatac aacgggagat
     8701 cctttcctac aacatgtggt atcaatccaa tcggatgatt acaccaccag ctttacaagc
     8761 gttcgtgcat taagaaatag tgatgtgttt gcagccgttc gaattattgc tagtgatatt
     8821 gcttcaagtc cgattcaatt ggtcaaaaac aatatgccac aagctgataa tgaactggtg
     8881 aagttactaa acgagaaacc taattcagaa atggatggtt ggcatttaaa gtttgctttg
     8941 gcggtcaata tgttgctaaa cggtaatagc tttgcagaga ttaaacgcaa cggtgaaaag
     9001 gtagaagaaa ttcacttatt acctaactca agtgtaacgg ttactcaatt agataatggt
     9061 acgttgtctt atcagattgg tgataaaaaa agacgtgtga agtctagcga tattttgcac
     9121 ttcaaatatt tcacccaaga tggtttaaca ggattaccac cactttatgc gttacgtgat
     9181 gaactgaaaa ttcaacaagc tggcaaccgc acattgcaca atttctttac tcgtggtgtc
     9241 agtgggtcag gtattttgaa ggttcataag tcagatttag acggatcagc aaagaacgca
     9301 attcgtgaaa agtttgaaga agccaacggt tcaagtagtg gagataatgc tcttagaacg
     9361 atcattcttg atgaaacaat ggactacaaa acattagaaa taaacaccga tgttttgaag
     9421 cttatcaatt ccaatgattg gaacacaaaa caaattgcca aggcgtttgg tgtaccgatt
     9481 gagcgtttag gcgttgaaaa tgaacattct agcaccgttc aaagtaatct ccagtatatc
     9541 caaagtacgc tgattcatta ctttaatgtg tttgtgagtg aattagatac gaaacttgaa
     9601 accaatatcc gctttaattc cgatcagtta ctagagactg atcctgaaac aaaagtaaaa
     9661 aatgtattgg atcaggtcaa agggtcactt ctcacgatta atgaggggcg gtcgaaaatg
     9721 gggctacccc ccatggatgg tggcgatcgt ttactagcaa gtttgaactt tacgtattta
     9781 gatacgttgg agaaatatca attaaaagaa caggaaggag ttacaccagt tgaataatga
     9841 agaagaaaag gaaaaacggc tgacagaaga agctgagcta acagccgatt ctcccaaagt
     9901 agagaaagaa aatgaaaaac aaccaacaga cggcaaaact atttcaggct atgcgttgaa
     9961 attcgggcaa ccgtcaaaag atttaggcgg ctttgtagaa gtgattacac ccgaagcatt
    10021 aaaagaggtg gatttatcaa atgtgttctt attatatgga cacgattatt caaagccact
    10081 agctagtgtc aaagcaggta cgttgaaatt gaatgtcgat gatacaggat tacattttga
    10141 agcagagcta accgacacga cctattcaaa tgatgtgtat gagaatatct caaaaggggt
    10201 tgttgattct atgtcctttg ggttcgtgct tggattagat tcattcgaca aaaaagaaga
    10261 tggcacaatt gaacgatcaa tagataaaat caaagcactt aatgaaatta gtgtcgtgac
    10321 cgttcccgct tatgattcat caaatgtcca agtaaataag cgttcttacg aatcgtttat
    10381 gagtaacaac caagcaaaac aaacaaacaa tagcttagaa tccacttcta aagcacaaaa
    10441 ggagagtaat aacatggaaa aaactttaat cgataacgaa aaaacagaat tgcgtgggta
    10501 tgaagaatat atccgttcac aaggcgaagt gcgtgatgga gtcactactg taaatgcagc
    10561 ggcagttgtt cccgaagaag taatcggtga agtctttgat ttgaaacgtt caaattataa
    10621 cttagctcaa tatgcaacag taaaaacagt atcaaatgga caaggtaaat atccagtagc
    10681 aactaaccaa caagcagtgt tagcaacaaa agctgaactt gctgaaattg gtgatattga
    10741 cgctgaaatg tttacttcag ttgattataa agtagaaact cgtgctggta agattgcctt
    10801 atcaaatgag gttgtggaag attcagcagt gaatattgta caagaggtca aagatcagtt
    10861 agcgaaattg gtagaaaaca ctgacaataa gcatatcatg gatttattaa aaacattcac
    10921 taagaaaacg gctgctacgt tggacgattt gaaacaacta tacaatgtgg cattagaccc
    10981 agcattaaat aaaatggtaa ttctaaacca aagcggatat taccacctag atacattgaa
    11041 agattcagat ggacgttaca ttttacaacc cgatgtgaca gcacctagtg gtaaatcatt
    11101 attcggtatg ccagtagtat tgattgcaga tacattgttt gccaatccta aagctggaac
    11161 attccctatg attatggggg atattgcaca atctatcttt gttgctcgta gaaatcaagt
    11221 aacgactcaa tgggaaaaat tcgattacta ctcacaagga cttgcagtga tcgttcgcaa
    11281 cgattataag aaaattgatc aaaatgcttc agtgtatatt gagtttacgc cagttgtaac
    11341 accaaaagta tagaaaaata ttgggcggcg gtttatccca ctgccttttt ttcattagga
    11401 gagataaaat ggtaattttg gatagcataa aaaaaagtat gcggatcgat cacactattg
    11461 atgatgactt tattcaacaa ttgattgata cagcaggcga atatataaaa agtgctattg
    11521 atagtagcgc aacggataaa gatatggata attatcagca atttgatttg gcggtgtcat
    11581 tacttactca acattggtat ttgaaccgtc aagaagccag cagcgaacgg ataccagtaa
    11641 cggtacaagc attagtacaa caaatgagag gtgcttatta tgccaatcat taagaatgta
    11701 aatgaattga ctgagagagt caagtttaaa aagacaaaac gggtaaaaga tgaagatggg
    11761 cagatggtgg atagtgaaga aactgtattt gaatgttggg ctaatgtgcg ttcacaaatg
    11821 ctaaaagacg tgcttgctag tgtaggtacc attcttgaag gaacgttgac gtttattatt
    11881 cgatacgatc aagattatga actaacaaac gatatgaaag tagcttggaa aaacaagagt
    11941 tataaaatta tttcaatcaa tgaaggaacg gcgtttaaag attatacaac gatcatagct
    12001 aaacagattt cttagaatga ttacacttgt aaacgttgtg ggtatttagt ataattaagg
    12061 tagtaaatga agggttagct gcctgaattt tgcaagactt agctagtcga aatctattgt
    12121 tggactgaaa attgtaggtg tggttgcaaa catgctggac taagaaagat gatagatgat
    12181 gaacgttcaa ttttaataca ggcatgctag gactgattgg atggttttgg caagctgatt
    12241 gtgtagaaat caaagaacac gtctgttgtg gacgtgtttt ttttgttata attcaaaaga
    12301 ggtgatttaa tggacaaagc agagtttaca aatttttatt ggaagtatta tctacattta
    12361 gaaaatgagt ttattaatac cacagatttt gtgatgctcg atgagaataa tttcaaaaca
    12421 ttctctattg aatatcaaaa actactacta gcgattggtt cggagtgtga gattattttc
    12481 aaagaacttt gcggatttga tagcagtagc aataaaagca taactgattt taaagctgtt
    12541 attaaatcta gcgaattaat gtgtttggat aatggtgtta gggttatgaa ttctttaaca
    12601 ttgacttcac taaaaccttt tggagatggg tggcctgaag atacaccaga ttggtggaaa
    12661 atatataaca gagtaaaaca tggtcgttct tcaaattata aggaagcaaa tttggaaaat
    12721 gtgttgtatg ctttagcaag tctatattta ttagaggaat atttgcataa gaaagtaata
    12781 ctagaaggtg aagtagactt tataagtcca gagtcaaatc tatttacgtt atcttgggaa
    12841 agaaaacatt cgtcgatgca aaaagtgacg tttgaaaaag tggattcaaa ttatgtagct
    12901 ccactcattg attttaaaga acaagaagaa atttaaaaac ccccacggct tattgaagtt
    12961 tttgtatgcc atattgtatg ccatttggtt taaatcgctt gaaaaatagt gatagataac
    13021 tctaattcat ttagtcataa acgctgtttt gataaccttt atagttcttt gatatgggtt
    13081 tctcattcat ggcggaagaa gaagaataga acaaaagaat gaattaataa gatataaaaa
    13141 aagggattgt gacaaacgtc ttgtcacaat ccctttttcc aaataaacga tgttcaatca
    13201 gctgcccctc ggcattcgct cgaaaaacgg cgaaatatga agaactattt tgccatttgt
    13261 ttctctaata atcaggtcaa aacgttgact tcacagtctc ttccttaaac ggtgttctcc
    13321 tgccaactcc tcggtcacaa gtcacaatat tcaacaaaca tgagaagctg ttttttgaaa
    13381 ttgttccttg tgactcggag ctgaacggca ggctcacgac ctctttctta aacggtgttc
    13441 aaaagcttgc atctgcggta ataagttcaa ccaaacgaaa attatgagga gctatttttg
    13501 tttggctgtt cttattactt gatgcaggac agctttttca caacctcttt tcgtttagca
    13561 ctaaatttcc ttaaaaagta gaaaagcttt cgaagcctcg tttgtttctt ttactgtata
    13621 ataaaagaaa agagtaacaa tcgctaaaag atataaacaa gagaaaggaa atacaagatg
    13681 attggtctga tttattttgt ggtgatcgtt ctggcgaaca cagttggcgc tatttccggg
    13741 atgggtggag gcgtgttgat caaaccgatt cttgatctta tcggcgccca ttctgttgca
    13801 gggatttctt tttactcaac agttgcggta ttcacgatgt ccattgtttc gactgtgcga
    13861 caagtatcaa gcgggaaatc actgaattgg cagattgtcg gttgggtatc tagtggagct
    13921 gttgttggag gagtagtcgg caacattgta ttcgaagttt ttttacagtt atttgaaaac
    13981 gagaagcatg tgcagctgat ccaaattttt ttgacggtat tgaccttggt ttttgcgttc
    14041 ttctacacca agcatcatca gccaaaattt catttgacgt catggacttg gtaccttata
    14101 tgtggggggg ttctgggctt tttagctagt tttttaggta ttggtggtgg accagtcaat
    14161 gtttctttgt tgatgctgat gtttgcattg ccaataaaag aagcaacact atattctcta
    14221 tctacgattt tcttttctca actggcaaaa ttggtaacga ttgctttaac aagtagcttt
    14281 atgcgctttg atttatctat gttgttttat gttatccccg ctgctgttat tggaggtttg
    14341 tggggagcaa gatttagcag ggttttgtct ccaaaaaaag tgacctttat ttttcaagta
    14401 attgtcctcg tggttctttt ggtcaattgt tacaatgcct ttgtcatctt acaaacatta
    14461 ggagaatcta gactaacgca agtcaaaatc actgaaacat attgttctaa atgaaaaaag
    14521 gagtatactg tgataaaaaa attcatttaa aaaaataaaa attggctggt gatttggatg
    14581 ttgcgggaaa tgcgtttagg agatgcagaa gcactacgag aaatcaatgc ggaacaatta
    14641 ggatatgatg taccattaac cgttacggtg agacaaatga agaaactgtt gaaggagcca
    14701 gaaaaaaatt ttttcttggt ctatgaagat cctacttcta aaaaagcagc tggctatgta
    14761 caagcggagt cctatgaatc gattttcagc gatccgatgt tcaatattat ggcacttgct
    14821 gtgtctaaac atgctgaaaa aagaggaatc ggaaaggcct tgatgactgg cgtagaagaa
    14881 gcgaaaagac gaggaatatc agcaataaga cttaattctg cagaatacag agtagaagca
    14941 caccgatttt atgaacgcat cggttatcat tccgataaaa tgcaaaaaag atttttgaaa
    15001 atcctgtaaa tttttatcaa aaaatacctg gtcatcaagc cgatacttga tgatcaggta
    15061 ttttttttga ctaagaaagt aattgcatca agaaacctga gatgatcaaa acgatggcac
    15121 caccaagccg attgcccatt tgagcaaaag caataagttc catccgatta gctgcagata
    15181 agacagctac atttcctgta ccacccatcg agttgttgca taagccagca gtaatagttg
    15241 attcgattgg atataaacca aacaaacgac cgacaaatcc tgaaactact gaaatcgcga
    15301 taacacttgt caaacataag acaacaaatt gccatgtcaa agcagaagcc aatacattta
    15361 gattcaataa cgcaataccg ataccggcaa gaacagctgc tgtcaagttt ttgacaatca
    15421 agttgttgaa catgatagct gcatcttcgt aatattctgg taataggttt gcgattttac
    15481 agataacaac gataatgatc atgaatgcgt aagcatggac tttaggaaca aaatagttac
    15541 agatttgtcc taagatgaag aaagtcaatg ataccatcaa accgacacct aattgtggaa
    15601 tggaaggttt tacatttcta ggttttccat cgccaaggtc gcctttttcc attaatttac
    15661 cattaccgtt gaattttggc attgattgtc ccgcacgagc aactaacgct gcaccaatga
    15721 tagctaggac atttgtcatt gctgtcgcag gaatcaggcg ggaaatgata gaagaagcgt
    15781 ccgtacctaa aacatttgca taaatcgttg ataaaggtac agctccggct ccaacaccac
    15841 cagccattgc gggcaatgag atatataaaa cggaatttgc aaaaccattt ccgataagca
    15901 tacctacgag tcctacggca aaaaaggaga cgaccattga aataagtgca acgggaataa
    15961 aacgaacaga agctttcatc aatagattac ggttcatacc aaggatgcta ccagtaatca
    16021 aggcagcaat ataaaagtct aaaaagccca tattgctgac aaagttttct gtggatttta
    16081 ctacatactc aggtaagatc ccaaatgtag caatcgcggc agaggcaaag atcgcaaaaa
    16141 cggagccacc acctaaataa cttttgacga tagggatttt tgttccgata aaatgaaata
    16201 aattaccgaa aatgaccaaa acagcgattg ggccaatcat attcgtcggt aattttccta
    16261 aagccattgc aatgatcagg acaaggacca tgatcaaata aaggggcaaa cttacgccgg
    16321 taatttttgt tgaccaaaac ccttcttttt tcttttcctc aactgcagtt gcggttgttg
    16381 ttttttgtgc catttttaca gatcctttct tattctgtat cgtatgaaaa aagccaaaga
    16441 gaagcggcaa aagagcagta gttggctggc tgctgttttg ccgcagtcgc ttttttcttt
    16501 ttagacgtca tacaaagttt ctgtatgttc ttttactata acaatcttaa tcttctaaag
    16561 atttatattc aggaacccat ttagcagatt caacagcttc tttgatatcc gtgatttctt
    16621 cacgattcaa tttttgtgca acaactgatt ttgcgacagt ttcagcaatc gtttgagaga
    16681 attcagtgat ttttgctact ggaggcaaga tagctgctcc tggttttgtt acatcaacga
    16741 ttccgcctaa tgctcgacta gcttgagaaa tgatttcagc atttactcgt gtagctgttg
    16801 aagcaatcaa accaagccct aaacctgggt acatcaacgc attatttgct tgaccgattt
    16861 ggtaagttac accgttatat tccacatcag cagcagggat acctgttcca actaaagctt
    16921 ttccgtccgt ccattcaatc aaatcttttg cttttgcttc tgccaatttt gttggattag
    16981 acaaaggcat gatgattgga cgtggggtat gagccgccat ttctttcacg atttcttcag
    17041 tgaatgctcc aggttgggta gaagtaccga tcatgatcgt tggatggatt gcttttacaa
    17101 ctgcttctaa gtttgtcaat tcatcagcat tagtgaattc tgagcgttta cgagcgaatg
    17161 ggatttgacc aggagtcaat ccttttgtat cttcaaacaa taaaccctgt ttatctactt
    17221 gatagaaatg ttggcgtgct tcttcttcag gtacaccttg acggatcatt tcatctaaaa
    17281 ggatgtttgc gatacctaca ccagcagtac cagcaccaaa tgtcaagatc gtttgatctt
    17341 tcaattgctc acctgatata ttcaatccac ctaatacacc agctagtaca acgattcctg
    17401 tgccttggat atcatcattg aaagtagtga ttttatcttc gtatttttct aagatcgttg
    17461 cagcatttcc tcgtccaaaa tcttcccaat gtaataacaa ttctgggaat aattcagtag
    17521 ctgattgaac gaattgatca atgaattcgt agtaagtttc gccttctaca cgagcatgac
    17581 gattccctaa gtataatgga ttattcaata attcttcgtt attagtacca gcgtcgattg
    17641 atacaggcaa gacttgtgca ggattgatac cagccgcagc agtgtagacc atcaatttac
    17701 cgatagcgat gtcaacaccg ttcacacccc agtcacccat acctaagatt ccttctgcat
    17761 ctgtcacaac gatcaaacga atgtcgcgtc catctgctgc gtttttcaaa cttgctttga
    17821 tattttctgg ttcgtcgatt gaaagaaatg cagcatcttg tggtttcaag aaaatttcgt
    17881 tgtattgttc gattgaatcc gcaacgacag gatcataaac gacaggcatg aattcaacta
    17941 aatgttcgcc catcaattta taaaacaatg tccggttcgt attgaataga ttcattaaga
    18001 agatacgttt ttccaaatcg gtctgtttgc ttaagtattg accgtaagct tggacagatt
    18061 gttgttccaa tgtttggaca gtacttggaa gcataccagt gattccgtat tttgcccgtt
    18121 cttcttttgt aaaagctgtt cctttattta gaaaaggatt atttaataag tttagccctg
    18181 taagcatgaa tgattcctcc taagttttaa tacaaacgaa gtataggagt cagatgagga
    18241 tatagtcaaa aagagcaggt ataataaatt ttatttatat gagtaataga gtcaaaagaa
    18301 atcaaaatag cggtatttgt tctttttata tcaacaatcc ttgcttgatt actatgcgta
    18361 tctcttatat gctaaagaaa gaaaagtaga agaataaagg atatgttaga tagataaata
    18421 gaatctctga agaataaatc acgtgatcgt gacgataaaa tacacttacg tcaggtgata
    18481 aaaaatacat aagtagaaaa ctaagaggag gaacagaaaa tgaaagaagt tcaattgatc
    18541 agaaaaagcg aactatcaga aggtggttgc aatgcatgcg gcgtagtaga agcaacaagc
    18601 tatacattaa agcttggtgc caacaaagcg ataatctctg agttgacagt aggcggtctt
    18661 gtggacagtc tggcgcttgc tgaaggtttt attggagaag atatttatga gatgttttca
    18721 gaaatccgtc agttaaaaaa aggggaaaac tgcatagagg ttcatcatga gagcccgaat
    18781 gtgagattca aacgtggtga taatgagatg atcttcaata atcatgtgtc cgatcacacc
    18841 gagctatatg gaatcgtgaa tcaaatacta actgagcttt ttggattggg accttatgcg
    18901 ttcaaggaag aaaacggtaa tccaaaatta aacgaagaat ggcaagaaac gattgaaata
    18961 caaagaaaca acccgcactt atttcaatag gcatccacag tatttcgtaa aagggacttt
    19021 gacacgactt ttgtcaaagt cccatcgttt tttatctatt tgtgttctaa acatttcttt
    19081 cagagtagta acaactggaa tgactgaaat gtaaacagat tatttgcctg tatcaatacg
    19141 gaagagtaat ataaagtgtg taaacgctat catattattc attcaatttt ttatagaggg
    19201 aaggcattga aatggaaaaa acgtatcaag caggtacaaa tgaaggaata gtggatttta
    19261 tcaatatgga agatttagaa atagccgctt cacaagtgat tccagctggt ggttatggtt
    19321 acatcagcag tggtgctggc gacttattta cttatcaaga aaatgaacgg gcattcaatc
    19381 atcagctgat tattcctcat gtattaaaag atgtagaatt acccgataca actacacatt
    19441 ttgacaaaga aacgctgact gcaccaatca tcatggcacc agttgctgct catggcttag
    19501 ctcatgtgaa ggcggagaaa gcttctgcaa aaggtgtagc agattttggg acgatctata
    19561 cagcaagttc ttacgcttct tgtacattag aagagataag agaagcaggc ggggagaaag
    19621 caccgcaatg gtttcaattt tatatgagca aagatgatgg gatcaatttg gatattcttg
    19681 aagtggccaa gcgtaatgga gcaaaagcaa tcgtattgac agcggatgca acagttggcg
    19741 gaaaccgtga aacagaccgc agaaatgggt ttaccttccc actgccaatg ccgatcgttc
    19801 aggcttatca atcaggagta ggccaaacga tggatgctgt gtacaaatct tccaaacaga
    19861 agcttagtcc aaaagatgta gaatttattg ctgctcattc tgatttgcca gtctatgtga
    19921 aaggtgtcca gtcagaagaa gatgtctatc gttcattaga atctggtgct ggcggaatct
    19981 gggtatctaa tcatggtggc cgtcaactag atggtggtcc agcagcgttt gattcgttgc
    20041 aatatgtggc agaagcagta gataaacgtg taccaatcgt gtttgacagc ggtgtacgac
    20101 gcggacagca tgttttcaaa gcaatcgcat caggtgcaga tcttgttgct attggcagac
    20161 ccgttattta tggattgtct ttaggaggaa gtacaggagt ccatcaagtg tttgatttct
    20221 ttaaaacaga attggaaatg gtcatgcaat tagctggaac acaaacagta gaagatatca
    20281 aaaaaataaa attgcgtgag aatcgtttta tttaagaaac aaatgacgaa aaaaatagga
    20341 gtgcggcaga tgatgccgta ctcctatttt tatagttcaa ggatgtcact aatagaataa
    20401 tttgaaacga aaaaataaaa ttgaaatatt ttttcatact taataaaaaa ggaagaatat
    20461 tcatgtaaac gattttattg tttttttctc ttttatacaa aaagggtcat aatttcaagt
    20521 aagcggatga attcatgagg attttttgca tactgtctct actatactta ggtcactaag
    20581 aaaaaggagt gatttattat gaaaaagaac atgattggga cattattact ttcaacactt
    20641 cttctaggcg gcttggcaac accagctttt gctgagggtc aagcgacttc aaaaggagat
    20701 attaccttta ctgaaccaac aaataccgtg gagcctctta accctactga tccttcaaaa
    20761 ccagtagagc cagcagatcc ggaaaatcca gcaactggcc agacaggtcc tttaacactg
    20821 gatgtagttc ccgaattgcc atttggtaca cacgagattg aaagcggaac aaaaacgtat
    20881 caagtagacg cttcaaaaaa cgatacacct tatctccaag tatcagatcg tcgaggtgta
    20941 ggtgcagatg gccaagcaca agggtggaac gtgacagttt cggtttcaga ttttgtaaac
    21001 ggttcacaag ttttgcaagg cgctgaatta gattttggaa catccactgt taaaagtacg
    21061 tcagataacg aatccactgg accaactagt caaactgtaa ctgggttgag taaagcaagt
    21121 gcagcgacac caatatttac agcagcaaaa gatcaaggtc tgggaacatg gctttctgtt
    21181 tatgatccag caaatatcac actgaaagtg ccgaaagcag cagctggaac attcacggct
    21241 gatttgactt ggaacctcgt ggctggccca gtagcataga gtatgaaata aattcaacaa
    21301 actgcggtga aaatactata ggtgaaggat tttttaccgc agtttttgtt gaaaggatgt
    21361 ggtggacaat gaatcaaagt aaacgactgt ttgtttcgtt tttttctatt ttaagtctat
    21421 ttttcatttt tccgtcaatc agccaagcaa aggattcagc aggagacttt ggaatcaaac
    21481 cggtatttcc agagaaccaa atagataaag caatcggtta ttttgattta ttagtggcac
    21541 cggaacaaaa tcagacacta gaagtaatta tcagcaattc ttcagatgaa gaacgaacat
    21601 ttgaggtctc tgtcaatccg gcggtaacga gtgatggtgg aacgatcgat tatagtcaaa
    21661 aaaacccaac gttagatgag acattgccgt ttgatgtccg tgacgttttg ctcattgcga
    21721 aaaaggaaat caatgtatca gcacatgcag aaacaactgt acctatagaa gtgaaaatcc
    21781 cagcaaaaag ctttaaggga cgtgtattag cgggtattca tgtttcacct aaagaagaag
    21841 ctgaaacaga aaatgctaca gaaggagcac aaatcaaaaa tcgtattgct tataatctcg
    21901 cagtcgtttt acaagaaagt caagagacga tagaaccgga tctgaaactt ttatcaggag
    21961 atttggacga ggtcaacgca aagccaactg ttcagcttcg tttccaaaat cctcagccta
    22021 ggattatcag caatttgata tttacgagca agatctttta tgaaaatcag ctttatattg
    22081 aaaatacgtc taatgcattt ttagttgcac caaattcgaa ttttcacttg aatcttgatt
    22141 tagctggtga taaagcaaaa gccggcgatt accgcgcaga aatcatagca aaatcaggcg
    22201 acagtaatga atggcgattt acacaaaact ttacgatcaa aaaagaaaaa gcacaaaaag
    22261 taaatgaaaa ttccgttttt gcagtacaag aacaatcttt cccatggctt tatgcaggat
    22321 tggttgctgc agttctagtg ttatggttga ttttgctgtt tttgtgggtc cacaagaaaa
    22381 agaaaaaagg agaaacaaca tgatgaggtg atttcgttga aaacatcgat cttattggga
    22441 gggattattg caagtttgct gttcttcagc tggcaagagc cagtccaagc agataccaaa
    22501 agttcaggta cgattacatt ttatgatcta agtccaattc cgcatccaag tggttcagtc
    22561 aatggcgcat caaaacagca gacacagcta ccgatttata aagaatctaa tcaacatggc
    22621 gtattcccgg agacgaatga acaaaagatc cgttttcttt atttgatcat tggtatagaa
    22681 ttatggcttc ttgcgtggtt gttgttatgg agaagaaagg aaaaggagga tgaacatgtt
    22741 gctacaaaat aaattttcat tttttcgctc cttctttttt acttcgcttt ttgttttaag
    22801 tatatttatc ttttcacctt cggcagaagc tgttggagca acgcctcctg ccatttctta
    22861 tgatccatac acgtatgaaa atatcgtacc tccaaatggt ggacgtagtg cagtcaatgg
    22921 cgggaagctt gtccaactaa ctggggctaa ccaattgcct aaattgactg ctacttattt
    22981 gaacgatggt tcaaatgatc cttggaaagg tggcacggtc cgttttcatt gggaaagcca
    23041 tgtgaaacta acaaatgtac ttgatttggt gaatatcact gcacaaggat ttgcatttgt
    23101 ggcgatcatc aatttgccgg aacatgtatc aagcagtgat gtattaaaag caattgactg
    23161 gaacaacgcc tacttgtata ttgccggtgc ccccatcaag gttactagcg gaagtttatt
    23221 atcagcagga agccatacct tgcgttttgg gatggggtcg tgggatacaa atactctatt
    23281 gacagatatt attgctatta ttacgcgagg cgaattcaac gtggacgata tcccgcttca
    23341 atttgatgtt acagctaatg tcgcgaagat gacagaaaac ggagataaat atgatacgag
    23401 tccaaacaaa attttgacca agggtaaatt acaaccttct atgtctaaaa aggccgtttt
    23461 ttctgttgat ttttatgatt cgaataatat tatccaagat cgtatgttca attcgatttt
    23521 agctccagct gcaggtagat tttatcctaa attaactggc ggaaacttga atccaggagc
    23581 atatgcattt tctgcaagtg cagagataaa tacgtggaat agttacatct caccaagtga
    23641 ttcaacagga aaatataata catttgctga taccacagaa tacgtggatg gaaataacca
    23701 gaaccttcca ggctctacca atagtcggac attgacgatg gggctgactg aaggacgttt
    23761 tgatcagttg ccatttagtc gctttgatcg cgtagtcaac tattttacag gaaaaaacgt
    23821 tacacaagga gcaatactga ctcatactcc agcaagcaca agtggattga atcagacagc
    23881 caatatcgtg tacagtggga aagatgcgca aggcggattg ctttctcctg ttgctttaaa
    23941 ggtattggag cagtacaagc tagacggaac ggttttacca acaaacttaa cagccgatag
    24001 tcagtgggga aagctccttt cttctgcatc gtaccaagtc acggccaatt ggtcagcaac
    24061 tgaactaaaa aagggttcga tccattacag gctttattcg aaaggaaccc aaaaattagc
    24121 gacaggatgg acagatcaac ttttccaaac gatccaatca aataacgggg gtgtaaatac
    24181 agctacttct acactacctg cattggcttc agggcaatat tattttgatt atcgcttggt
    24241 ggacgatgta cttactaaca tgtatccgtc ttttgcgtac aaatggcagt ctgaacaagc
    24301 tggaatcacc actcttcctc aaataacagt ggctaatttt ccatctgtga gtgcagatag
    24361 tcagctaaaa aaccttagca gacaacctga gacaggagat cccctgtctg ctctatcagg
    24421 agatacgatt caagaaaatg tgacattcac gttaacaaaa tctggggatg cgataaccga
    24481 caaaaaaatc acgatctcac ttcctgaaaa tactacttat atcaatggtt ccttaaaatt
    24541 aaatggtaca actatcgcag ataccggcat tcaacaagga gtttctgtcc cagcagatct
    24601 attgagcaaa ataggagata ccattcatct tacttacaac tatcaattaa atactgtaga
    24661 tacttcttta caatccgtca gcatactgac aaaggcagct gtactttcca gcaatattac
    24721 attagcagat ggtgctaagc ttcctaaccc agttgtccaa acatctgcta aaacaattct
    24781 tgtccccaaa caagaattaa cattagttaa tgtaccagat gattttacat ttggtaacga
    24841 tctccccaaa ccgttgaaga caagttatta tgaatcaaaa ggcgattttt cttttgatgt
    24901 gcgggatact cggcttccaa gcacgtctcc ttggcaactg acaggtacat tgacttcttt
    24961 atttaagaat gatcaaggtc aagaattaag cggaacaaaa ttatacttca atcattctgg
    25021 atcaaagcaa ttgattcagc aaggtcaaaa cacgttgatt tatgagagtg atggaacagc
    25081 taaaggagag gttctcgttg actttcccga taccgatggt ttactcttag aggtaaatag
    25141 cagtacgaat gctcaacctg gcgcaacgta tcaaggcatg gtcacatgga aactgacagc
    25201 tgggccaacc agctaagtga ttagaatttg tatggatctg tgataactgt cacagatcct
    25261 ctttttcaag gatgagaagg aaacttctgc tgttaaaatc agaaatgctt gaaggagtgg
    25321 acttggaact tttcagtttg cttgaaaaaa atgaacaacg gcaaatcaaa cttttccaac
    25381 ttttattaga ggaaaaagga gaggtttccg ttaataacgt acttacccgc ttatcagttg
    25441 atcgggctac cttgaaagaa gatctgtttt atttacaagc taacttggaa aatatcaaac
    25501 aattggaaat tgttatccaa gaagggaata ttttattaat cagacatgga aatatagcag
    25561 cagtcgatgt ttattatcat tgttacttgc gctattctac aaaatatcag atacttcttt
    25621 atctgttgga aaatggctca tttgatcggc agaaacttgt catggaactt aatatcagtt
    25681 cggctacttt gtctcgcaga gtgaaggaat taaatgattc tttaaatgag tttcatctga
    25741 aaataaaaag cgggcggctg accggtccag aaagtcaaat tcgttatttt tatttccaat
    25801 tattttggtt tggacgtccc tatcttgaaa acaaaaaaga atacagtgat ttatcaggaa
    25861 acactttttt agatctgctg caaaaagagt ttagttttgc tattacagaa gaaggacgat
    25921 tgaaatttga aatatggctt tccattatga agaagcgaat gcgctgtacg aaaaaatcca
    25981 aaccagatta tagcaatctt cctgttggat tgtttcaaac gacacaattt ttattggacc
    26041 tccatacatt tttgacccgt tacttctttc aatatgcctt tccttggaat caaaatgaaa
    26101 cgttggtttt ttatcttttc atgatcagta actttacatt agatacacat aactcgcatg
    26161 tccaggagtt tttgataagc gaaaaataca aagacgacgt agtagctcaa ttgaatcgaa
    26221 cgtttaaaca atcgatagaa gaatatttct ctttttatgt ttttccagaa gagatttcgc
    26281 aaaagattgc gttgacagtg atgcaaaatc acgctcattt tgcctatttt agaggatgga
    26341 tgaatatttt tggccaagaa ggattaaaac agcggttaac tgaagtagaa gataaacaat
    26401 tcctggtttt atgtgaaaga ttggtcagac aaacatttgc tattgttgat tttactgaag
    26461 tagacgagga aaatgcccgc tttgaattat ttgctcgcta tgcatctatt ttacggacgc
    26521 tgatcaagca gttagaatta cgtctgcaaa ttgcttatga tcttccttat gaaaggctaa
    26581 tggcggatat cgtgaaagaa atcatccagg aacaattgct gaatacgatc aaatatgatt
    26641 tagtcgaata cgagaaagaa aagcagtatg atatcatttt gactagccaa ttgaaagagt
    26701 acccgtcaca gaagtccgct aaagtttttg tattcactag caacgaatat aattatgatt
    26761 tcccctattt gaaccaattt ttaaaagaat gttatttaga aaaattgaat ttaaggatga
    26821 ataaaacatg aaaatcgtga tcattggtgc ttcccatgca ggcatcacag ctgccctaaa
    26881 tctaagaaaa cttcaaccga aagcagaagt tttactgatc gatgaagatc ataaagatgg
    26941 actaggctat gtttcaaatg gaatcaacct ttatttaaaa ggaaagatac gtagtttagc
    27001 tgaagttgcc cataacatgc ggactcttca agaaagtggg gcaaaactga tcaccgaatg
    27061 gagagtaact gaacttgatc cagacaaaca tcagctcatc ctcgcatcca aagaaggaaa
    27121 aaacgagacc ataacctatg ataaattaat cgtagcgaca ggttcctctc ctgtcacctt
    27181 gtataaacaa attgaagctg aaaatgtcta cacctataaa aatctagtgc aatctaagca
    27241 agtacttgcc gctttaaaag aagcaaaaga agtagtgatc ttcggtgctg gttacattgg
    27301 attggaatta gcagatgctt tgagaaataa aggatacatt attcatctag ttgattatat
    27361 gccgaatgtt ttatcaagat atttcgataa ggatatgatc aacagttttc aaaatcagct
    27421 gcaaactaag caaataaatt tttatccaaa tgaattcttg atcgattgga aaaaatcaga
    27481 agagaaagta gtatccgtac aacttctgtc tcaaacaata aaggcagata tggtcatttt
    27541 ttctgcccaa actcgtccta atacgacatt gttaaaagaa aaagtggctt tgtatgagga
    27601 tgagactgtg atagtcaatg aatatctgca gacaagtgat ccggacatct atgcgattgg
    27661 agatatcgtc ccagtcagtt ttgacaaaaa taaacggcat ctctttttac ctttagttac
    27721 tcgagctgta catatggcaa gagcagtagc gctaactttg agtggtcagc caaccgctta
    27781 cgacctcaga caaaagataa cagcagcagt cattatagac tactttttag gaactgtagg
    27841 gctgacagaa gatgaagcgc cattcttgga acaaaatacc ggttcgtgtt caggagaatt
    27901 tgatctattt ccacaatacg acgaagaaaa caaaacagta aatgcaaagc tgatctacca
    27961 tccggataca ttagaaataa ttggcggaca gctgataagc caagaattct tattatcaga
    28021 tttgaacctg cttgcggata tcgtgaaaca caaaactacg attccacagc tagcagttga
    28081 ggattttgga tttctgtctg aatacacctc tcgttttcac tacctcaatg agttagcttt
    28141 taaagtacta gctaagagag caaatagcaa tagagtagat aaaagactgt gagaaatatc
    28201 acggtctttt attgtgtggg gatataataa atattaatca atcgagaata aaaaattcat
    28261 ataatgaaat agcggaagaa ttttttgagc ataatattcg ccaaaaattt atcaagtgat
    28321 aaaattgttg tataaaatga ttatttcagg aggaggagtt aaatgttttt agcattaaat
    28381 gaaattatgc attcaaagtt acgttatgcg ttagttgcag gggtgatgtt tttgatcgcc
    28441 tacctggtat tttttttgac tgggttagct tatggcttag ctcaagataa tcgaacagca
    28501 gtcgacaagt gggaggcaga ttcgattgtg ctttcgaaag atgcaaattc aaatctaggg
    28561 atgtccatga ttactaagaa gatagcagaa gaagtcgaag gtgggaaagt ggcgtatctt
    28621 gcccaaacac ctggagtcgt gactagtaaa gacagtacag aagaaggaaa aatcaatgtt
    28681 agcttcttcg gtatcgataa aaatcaattc atcatgccca atcttgttga aggaaaagct
    28741 tttgataacg atgatgaggc agtaggcgat attagtttga aagaagaata tggtctagcc
    28801 ataggagata cagttaagtt atctggtagt gacaagacgt ttaaactaac tggctttacg
    28861 gatcatgcca aattcaatgt ctcaccagtt ctctatacaa ccatcaatgc ttaccaagaa
    28921 atccgcttcg aaaaagaaga tacaagtgaa aatgcacgga ttaacgcaat agttgtcaga
    28981 ggtaaaatca gtaatttacc tgaagattta gaacagatca aaatttctaa attcatcaat
    29041 gaattacctg gatataatgc gcaagtattg acttttggat ttatgatcgg atttttgatc
    29101 gttattgcgg ctattgtgat cgggattttc atttatgtac tgacgatgca aaagattaat
    29161 atctttggtg taatgaaagc acaagggatc acaggaggat ttattgcgcg ttctgtagtg
    29221 gcacaaacat tcattttatc atttgttggt attcttcttg gtctattagg aacagtggga
    29281 acatcccttg ttctacctga tgcggttcca ttccaaagca attggctgtt ttttggcgtc
    29341 attagcttgt tgatgttagt agtagctgtg ttaggcgcac tattttctgt tcggacaatc
    29401 gtaaaaatcg atccgttgaa ggcaattgga taagaaggag gaagaacatg aaagcaatag
    29461 aatttaaaga agtagaaaaa agtttccaag atggcgacca agtagttaaa gcattgaaag
    29521 aaacaaccat gagtatcgat aaaggggaat ttgttgcaat catcggtcct tctggttcag
    29581 gtaaaagtac ttttttgact ttggcaggcg gattgcagac gcctagcagt ggctctgtat
    29641 ttatcaatga tcaagaattc agtaatcaaa aagaaagcga acgcgttaaa ctacgtttta
    29701 aagaaattgg ctttattttg caagcttcca atctggttcc ttttctgaca gtaaaaaagc
    29761 agttattatt agtagataag attaaacatg aacatcgtca aaaagaagca aacgaattat
    29821 ttgagcagct tggcgtggat aagttgatca acaagtatcc agaagaacta tctggtggag
    29881 aacggcaaag ggtcgcaatc gctcgtgcat tgtacaatga tccgtccatt attctagcag
    29941 atgaaccaac tgctagtttg gattcagaaa aggcacgaga agtagtcgat atcttggcaa
    30001 aagaatcaaa agacaaaaat aaagcaacga tcatggttac tcacgataca cggctgattg
    30061 acaaatgcga tagaatattt atgatggaag atggtgtatt gaaagaaaaa gaataaccaa
    30121 aaagcaggtg gaaaaatggt ccatcgatac aacaaagata cacagcaaaa aattttgaaa
    30181 gaagcgactg acttgtttat gtccaaggga tatctcggca cttcaacaag agaaatcgca
    30241 caaaaagttg ggatcaccca gcccaatctc tatcattatt tcggtgataa agaaaaacta
    30301 tatacggcag ttttagaaaa ccacttaaaa gatgttggaa aagctttgag agaaatcgtt
    30361 caaacaagcg aaacgggatt tcaagtaacc ttgacaaaaa tggcgcagtt tttgattgag
    30421 acacatctgg tcgatttgtt tctgatgctt cacgatcttg aaagcaatct ttcaaaggaa
    30481 acaagagatc atcttttctt tctatggaag aaaaattatc gtgaaccttt tgaagaaatc
    30541 ttttcagcaa accaatctgt gctaagaaat gggatttcaa aagaaatcgc agcaaggcat
    30601 tttttcttag tgttagcacc ttacatcact cggtcgaaaa gacctgtgga agaaccgcta
    30661 aatgtggcgc aattgatcga tttatatttg aatggtgtat taggagaagc gtaaagctgt
    30721 tatataggga gaaaatagtt aggtgaagaa gcaagatatg acgaagatcg ttatatcttg
    30781 cttctttata taggtagttt ttcataaaga caaatttgtt attctctcat gattatcagg
    30841 gtatattttt atttattatg tcggtgttcc aggaagtgtt ttaatttaat aacgaagaaa
    30901 ttaattgttt gtagaatgaa ggaaactatt tattgggtag atgaatcacc acgaatagtc
    30961 aaaaggagat ttcaggatat gccaaaaatg aaaaaaatca ttttagcagt aatgttattt
    31021 tctacggtta tatcttttac agcccctttt ttgcttactt aagctgattt taagttttgg
    31081 cccttagtct ggatctacga ggtgagagtc aagaatgaaa aaaataattg gagcttttgt
    31141 atttgcaatc attatcatta ttatctgttt tcaaatagat gtttttggtc aaaaaaagat
    31201 agttggacaa acggtcagca gtacgaacag tacggttaca aagacaaagg aatcatttga
    31261 tgatacagaa caagagacat taatcaaaat cggaaaaaat gaccgtacac tatatacaga
    31321 tgactcgcta actaccccac ttgcaactat aaacggcgga gagctggcgg agtttctttc
    31381 tgaaacaaaa aatgcttatc agatcaagac aaatgatggc tatactggat atttagccct
    31441 cgaagatgga accaaagtca caaagaatat acaaacaaaa ccggaagagc tatctgatgc
    31501 agtgattgta ttagatcctg ggcatggcgg aaatgatact ggggcattaa gcaatgatga
    31561 acaaacagaa gaaaaagata taacattaag tacagccata aaagtaaaaa aggcgctaga
    31621 ggatgctgga gcaacggttt atttgaccca tactacagat gaattagtac agcttggaga
    31681 tatatgcgac tacagtgaag aaaaaaaggc agatgtcttt atcagtttac acgcagactc
    31741 tacggaatat gcgaatgaag caactggaat cacgacatat tattactatg gacaagagga
    31801 aacattagca cagacaattg ctgactcctt cactgatctg cctctggatt caagaggaat
    31861 ctccactggg aactatcaag ttcttcgaga aaatcttcaa ccttctattt taatcgagat
    31921 gggttatatg aataatgata gtgatttaaa ggaattagta acagacagtt atcagcagca
    31981 gattgctgca agtttgacac aagcattgac cacttatttt cagtaataaa taagcccagc
    32041 caaacaaaaa tagctcctca tatttttcgt ttggccgggc ttattatcgc agatgcaagc
    32101 ttttgaacac cgtttatttt tttagtagct tcaaagcttt ttctgtattt gcaaagtgaa
    32161 gtttggcata tttttcaagc ataggcatgc cgcctttctc caatagtttt gccgctactt
    32221 ggtcagattt cgttccagct cctgatggaa gggtgattcc agcttcagct gatgctttgt
    32281 gtaactcttc caaaaagctg gcacgactga tgatagaagc agcagcaaca gctaaatgat
    32341 attgttctcc tttagtaacg aagtacagtt tttcttgtaa ttgatttttt tctaaacgcg
    32401 catatttttt aaaatttgct tccggcgtaa actgatcgat caaaatcgct tctggttttt
    32461 ctggctcgat tttttttagc agcaaatgga tcgcttgatt gtgcaaagcg actttcatcc
    32521 gaacggcatt gtattttggt tggatctcat tgtatttttc tggagataag acaagcagtt
    32581 tataggggat ggtttttttt aagatgtcag caagctggat gatttggcta tcttttaact
    32641 cttttgaatc tctaacacca agtttacgta attctgcaag ctgatcacgt ttcacataag
    32701 cagcacatac tgttagcgga ccgaaatagc tgccatttcc gacctcatca ctacctaaaa
    32761 cggataattg actgatattt tttggcaaag aagaggctgt ttttgctggc ttggcttgtt
    32821 tatcctgtgc ctccccccat ttatcagctt ctattgctgc attcgtacct tgaaacatca
    32881 cttttccgga tgtataagcg gtaatggtcg tcccgtcttt ttttgcagca aagctagtat
    32941 aaggaatctg tttatttaac aaataagagc gatagtatgc tttcatttga ctaagttggt
    33001 cgtttgttgc tttgataatg acgttgctca taaaattctc ctcgttttta tacataaaac
    33061 tataccgcaa ctcacgactt ttcgttttct aagagattgc gagacatagc atttttttaa
    33121 agtttatcat ataatagtta agtatgtgaa aaatatttgt ggagaacggt gaaagaacca
    33181 acttcatggt acactaaata aaactttcat caaataggag gaaatgattc gtatggcgca
    33241 cgaaaaaaca cgctacaaag cggtgattgc caaccaaaca tatactatta tcggacggga
    33301 aaccaagcat catatggata tcgtaacgaa gctgatcaat gaacagttag cagaattgaa
    33361 acaattatca ccacaaatgg ataatgaaca agcagcaatc ttgatggctg tcaatgcgct
    33421 ttcggatcaa ttgaagaaac aagaaagaat cttggagctg gaagaagaaa cagcagaatt
    33481 aaagaaaaaa atgatcaaat ttacagagct tgaaaatcgt gtgaaacgca ttgaagcaat
    33541 cgagaacgaa gctcgggaag tattgaaaga aaatggtcaa gcagatcata ccattaaaaa
    33601 tcatgtggaa gcacaacaga ttttaaacga gaaacgaaaa ggacaaatta aacaaaaatc
    33661 ttctaattaa gatcctttta tcaaaataag aaaaatttaa aaaaagttag aagagaaagg
    33721 aacgtagcat gctgagtttg ctgattttat ttttattgct aattgcgttt ttttctggag
    33781 cacgtcgcgg gtttgctttg caaatcatat tcgcgattgg atatgtattg tcatttattg
    33841 ctgcccagca tttttacaaa ccattagcaa gtcatttgga attatacatt ccatacccag
    33901 ctgtcacgcc aacatcaaag ttagcatttt ttgatcaggt atttgccttt cacctagatg
    33961 aagcttttta tgcaggaact gcttttctga ttattttgct aattggctgg ctgcttaccc
    34021 gatttgtggg ggtttttgtc catggattga cgtatgtgcc gatcttgaga caggtagact
    34081 ggattgcagg aggcatcctg tcactaatca tggcttacgt tactatattt ttgattttgc
    34141 agttacttgc ctttgttcca ttagatgtgg tccaaaatca attcggcgag catagtttcg
    34201 ctcgctttat cgttgagaga acgccatttc ttaccaacaa gatgtatgag ctttgggtaa
    34261 cgaatatttt gaattaagga aaatagtgct ggaaaatatt tcgttttttg tacagagtag
    34321 attatttaag aaggctgcgt agcgtatgtc cggcacagct cttttttcat agtgaaagat
    34381 aaggtgataa aatgaataac cgaatccttg aaacattgga atttgaaaaa gtcaaacaga
    34441 tggtcagaca atttgtagtg actgcacaag gaaaagaaga actggcagaa ttggtaccag
    34501 tgagcgagga acaaacgatc acaaactggc ttcaagaaac tgaggatgga ttgaaagtgc
    34561 aacgtctgcg aggcgggatc cctataccta aacttgaaaa tatccgtccc catatgaaac
    34621 ggattgagat cggagcagac ttgaatggag tggagcttgc acaagtttcc agagtattgt
    34681 ctacgacaag cgaattgaat cgtttcattg atgatcttta tgacagtgaa atcgaatttg
    34741 cacgtttgta tatgtgggca gatcagttag tcacgatccc tgttttgagc cgccgtttaa
    34801 aggaagcaat cgatgaagac ggacgcgtga ctgatgacgc ttctcctgag ttaaagagca
    34861 taagacagaa cattcggcga agtgaacaag ctgttcgtga acagttagat gggatcgtcc
    34921 gtgggaaaaa tgcaaagtat ttaagtgatg cgatcatcac catgcgaaat gaccgctacg
    34981 ttatcccagt caaacaagaa taccgcggtg tatttggcgg tgtagtccac gatcaaagtg
    35041 cttcaggaca gactttattt attgaaccaa aacaagtggt ggatttgaat aatcgtttgc
    35101 gtcaatacca aatcgctgaa cgaaatgaga ttcaacgtat tttaagtgaa ctttctgctg
    35161 aacttgttcc ccatcgtcaa gaaatcatcc acaatgctta tgtgattggt aaaatggatc
    35221 tgatgaatgc aaaagcacga tttggtaaag aagtaaaagc tatcgtgccg ggaatcagtg
    35281 aagacaatca tgtcgtcttg aaacaagcaa gacatccact gatcgatcag gaaaaagtag
    35341 tttctaatga catcacaatc ggaaaagact atcaagccat cgtcattaca ggacctaaca
    35401 caggtgggaa aacgatcacg ttgaagactt taggtctgtt acaactgatg gggcaagcag
    35461 gattaccgat tcctgcaggt gaagaaagtc aaatcggtat ttttgaggaa gtatttgcgg
    35521 atataggaga cgaacagtcg attgagcaaa gtctatcgac cttttcttct catatgacga
    35581 acactgtaga tattttatca aaagtcaatg aaaaaagtct ggtattattt gacgagctcg
    35641 gtgcaggaac agatccgcaa gaaggtgctg ctctagcaat cgcaatctta gatgatcttg
    35701 gtaaaaagtc tgcttatgtg atggcaacaa cgcattatcc tgaattgaaa gtttatggat
    35761 ataatcgggc aaatacgatt aatgccagca tggagtttga tgtggataca ttaagtccaa
    35821 cgtatcgttt gctgattggc gttcctggac gaagcaatgc gtttgaaatc tctagccgat
    35881 taggtcttga tacagaagtt attgatgaag cgaagcagtt gatgaatgat gagagccaag
    35941 atttaaacga aatgatcaca gatttagaga atcgccggaa aatggcagag accgaatatc
    36001 tggaaatgcg tcattttgtt tctgaggcac aagaacttca cgatgatttg aaagaagctt
    36061 acagctattt ctttgaagaa cgtgaaaaag aaatggaaaa agcaaagaaa aaagcaaatg
    36121 aagtcgtgtc agaggcggaa gaaaaagcag aaaaaatcat tgctgatatt cgtaaaatgc
    36181 aacagcagat tgggcaagga aatgtgaaag agcatcagtt gatcgatgca aagacccaat
    36241 tagcaaatct acatcaagaa gaaactttga agaaaaataa agtattgaag aaggcgaaag
    36301 aacaaaaaac gttgaaaccg ggagacgaag tgttggttac gacttacggc caaagaggga
    36361 cgcttttacg taaaaacggg aatcagtggc aagtagaaat cggtatatta aaaatgaatg
    36421 tgtccgaaga cgagctgacg cctgttgctc ctcaaaaaga gccgacacaa cgagttatcc
    36481 atgctgtacg atctgaatct agtagtcacg tgccgaatca attggacctt cgaggaaaac
    36541 gatacgaaga agcgctaagt gaggtagatc aatatttgga ttctgccatt ctagctggtt
    36601 atcctcaagt gactattgtc cacggaaaag gaacaggcgc acttcgcaag ggaattactg
    36661 attatcttaa aaaccatcgg agcgtcaaaa gctttgagtt tgcaccggca aaccaaggcg
    36721 gaaatggtgc gacaattgtt aaatttaagt aaagcttact aaaggtaagc agatggttcg
    36781 ctatccaagt atagaaatgc tatactagtc atagatttta aaaatggagg tctttgatat
    36841 gtcacaagta attacagata agacatttaa tgaagaaaca gataaaggac ttgtattgat
    36901 tgatttttgg gcaacttggt gtggtccatg tcgtatgcaa gcaccgattt tggatcagtt
    36961 ggaacaagaa tatgatgaag aagaattcag aattgctaaa atggatgtgg atgaaaatcc
    37021 agaaactcct caacaattcg gtatcatgag cattccaaca ttaatgttga aaaaagatgg
    37081 tcaagtagta gaaaaagctg ttggtgttca ttcaaaagaa caactacgtc aaatgatcga
    37141 tcaatattta taaaaactta cagcaaaaat aagctgaggg tgggactgaa tccagttccg
    37201 tcctcggttt ttttgcggtt gagttaaggt tattcaaatt aatatttttt gagtataatt
    37261 acgttgtcag gctggattgt ttttttgaca gacatgctat aataacttta attgaatatg
    37321 aaggggagta ccagatgaac gaacgaatca aaaacaaatt agctttactt cctgatcagc
    37381 ccggctgtta cttgatgaaa gataaaaatg gaacgatcat ttatgtcggc aaagccaaaa
    37441 tattaaaaaa tcgtgtccgc tcatatttta ctggtagcca taatacgaaa acggaacggc
    37501 ttgttagtga aatcgaagac tttgaataca tcgttacaga atcgaatatc gaagcattgc
    37561 tcttagagat caatctgata aagaaaaatg atcctaaata caacattatg ttaaaggatg
    37621 ataaaaccta tcctttcctt aagataacga atgaaaaata tccccgtttg gtgattactc
    37681 gtaaggtact aaaagacaaa gcactgtatt ttggaccata tcccgatgtc ggtgcggcga
    37741 atgagacaaa gaagatcctc gatcgattat ttcctctgag aaaatgcaaa cctagtcaga
    37801 cgaaggagcc ttgtctttac tatcatttgg gacaatgtct gtgtccttat tatttcgacg
    37861 tagatccgaa agtctatacg aaaatcgttg atgaagtgaa aaacttttta aacggaggac
    37921 acgaaaaaat cgagtcagag atccatcaga aaatggaaaa agctgctgaa aacatggagt
    37981 ttgaaaaagc tgcagaatac cgagaccaga tccgtgccat tgagacaatc atgactcgcc
    38041 aaaaaatgac aaatactgat ttactggatc gagatgtatt tggttatgcg attgataaag
    38101 gatggatgtg tgtccaagtg tttttcgtaa gacaaggaaa gctaattgaa cgagatgttt
    38161 caatgtttcc tttctataac gaagcagaag aagatttctt gacttacctc ggtcagtttt
    38221 atcaggaaaa tgagcatttc attcctaaag aagtcttgat tcctgatacg atcgacaagc
    38281 ccagtgtaga agctttgtta gctacgaaag tgctgcaacc aaaacgagga gaaaagaaaa
    38341 aattagttga attggcgagt aagaatgcct cagttgcttt gaatgaacgg tttgatctga
    38401 ttgccagaaa acaggaacgg acgatcggag cagttgaacg tttaggaaac gcgatgaata
    38461 tcccgacccc aatacgtatc gagtcttttg ataactctaa tatcatgggg acgaatcctg
    38521 tggctgcgat ggtcgtattc attgatggaa aaccggcaag aaaagaatat cggaaatata
    38581 aaataaaaac agtcaacgga ccggatgatt atgcatcaat gagagaagtc atctatcgca
    38641 gatattcgcg agtgattcgc gaagggttgc ctttacctga tttgatcttg atcgatggag
    38701 gaaaaggaca agtagatgtg gcaaaagatg tcttagaaaa tcaattagga atcgatattc
    38761 ctattgctgg catggcaaaa aacgataaac ataaaacaaa tgagttgcta ttcggtacag
    38821 atttggctgt agtaccgctt gagcgaaatt caccagagtt tttcttactc cagcgaatcc
    38881 aagatgaagt ccatcgattc gcaatcactt tccatcggca gctacgcagt aaaaacagtt
    38941 ttgcttcccg attagatgaa attgaagggt taggaccaaa acgaaaaaaa atgctgctta
    39001 aagagttcaa atcgttgaaa aatatcacag cagccacgac agaagaatta caagaaatcg
    39061 gactgccgaa aaatgtcgca caaaacgtat ttgataaatt acatcaggaa tgaggaaagg
    39121 gcactttaca aagaaagtaa agtgtcctat cattttatgt acaacaaaaa gagcgtttac
    39181 gttttgttgg taaataataa atcgcttacg tctggtgtct aaggatatat gtgatttctt
    39241 gaagaaggag tgaagaaatg ttagaagtca aggatcttgt taaaactttt gggaactaca
    39301 cagctgtcga tcatgtctct ttccaaatcc ccgatggaaa aatcatgggg ctaattggcc
    39361 aaaacggggc tgggaagaca acgacttttc gtttgatctt ggattttttg acacaagacc
    39421 aaggagaagt gttatggaat ggccatcatc ttggggagaa agattatgat gtcatcggtt
    39481 atcttccgga ggaacgggga ctttatccga aagtaacgat tcaagaccaa ctgctttatt
    39541 ttgcagaact tagagggaaa tcaagaaaag agattgaacc aaaaatcgac ttttggatgg
    39601 aaaaatttca ggtaaaaggg aaaaagacgg ataaagtaaa atctttgtct aaaggaaatc
    39661 agcaaaaagt ccagctgatc gccacgttaa tccatgaacc taaattgatc attttggatg
    39721 agccattcag cgggttagat ccggtaaatg ctgaattgct aaaagatgga atattggaat
    39781 tgaaaaaaca tggttcatgt gtcatttttt ctagccacaa tatggataat gtagaaaaaa
    39841 tctgcgatca tttgatcatg ttgcgaaacg gaaaaatggt tttgaatgga aaagtacatg
    39901 agattcgtga atcatttggc cgtacgaagt tattcttgga atctggttta agccaacagg
    39961 aagtagaaga aatcgaaggc gtgaaaaaag tgatccttca tgaagacaat acgttagaga
    40021 ttaccttaga agatcctgaa gctgggaaag aaatctttac tcgtgcgact cagttcggct
    40081 acattcctat gtttaaccag caaccgccaa ctttggaaga gattttcaaa ttgaaagcag
    40141 gtgaaatcga tgcgtaagtt ttggattatt accaaagatg tctatttaaa aaatgttaag
    40201 tcgatttctt tcttgatcat gatattagtc ccttttatca tgctgggtat catttatgta
    40261 gcaggaaact ttgctcaaca aaatagtgat gtcaataaaa tcggcgtatt ggctgataat
    40321 gagcaaatca gtcagcaaat gagccaagtc aaaacagatg attttgagtt caaagcagtt
    40381 gattctgaag aaaaagcaca aaaagagctt tcggaagaaa aaatcgatgc gtatttagta
    40441 gtctcaactg agaatgataa gataaatggg atgctttata gcgaaaattc attaggacaa
    40501 agtacacaac tactgataca acaacaactg actggcttgc aatcgatgct tcgagcgagt
    40561 caattaggca tttcaccaga agaagtagct agcttgagcc aacaagcaag tttgactcga
    40621 caaaaagtca attttgattc aaaaggaaaa atgacagtcg gagaagataa ctcgacagtc
    40681 caatacgtcg taagttatgt agcaacgatt gtcttgttca ttattatttt gacgtatgcg
    40741 cagatcattg cacaagagat cgcttctgaa aaaggcacgc ggatcatgga agtgatcctg
    40801 tccagtacga ctgcacaaaa gcatttttac ggaaaattaa caggtgtttt acttgttgca
    40861 ttaacgcaaa tggcattata cggtattatt tttggtatag gatacagtca attcaaagat
    40921 atggaaatgg tcaaaaatat attagacggt atctcattgg ataacttgat tggaccattc
    40981 ttgtggtatt ctcttctatt catgatcttt ggcattttga ttttttctgt tttagcagct
    41041 ttatgtggct ctttggtcaa caaagcagaa gatacagcaa aagcaatttt gccagtgact
    41101 tatctatctc taggcggata catgcttgga ttgatactag gagcttcaga tccaaacaat
    41161 atcgttattc ggatcacttc gtatattcca ttcttatctt catatattat gccggttcgc
    41221 ttagcaaatg agacagtcgg tatgggtgga gcattgattt ccttgttggt acttgtagcc
    41281 gtaacattct tattgatgat cttgtctgca aacatgtaca aatcgaatgt actcgtttat
    41341 agcgaaggcg gattatggag ttcattgaaa caatcgattt caattatgag gaatgaacga
    41401 aaaaaagcat aaaacaatcc taaaatacag aaggaagctg gacatctatt gttcccagct
    41461 tctttttgta tagacctatt ttatttattt tactatattg gatatatatt cattgaaaat
    41521 agtgtataac aactttataa tattgagtat accaattttt ggaggcgttt taatggaccg
    41581 ttatttagat ttactttcag aaaaattccc ctcaacggag gatgtcatca cggaaatcat
    41641 caatctcgaa gcaattttgc agcttccaaa aggaacagag ttatttttga gtgatataca
    41701 tggcgaattt cctgcttttg atcatattct gcgtatcggt tcaggaaatc ttaaagaaaa
    41761 gatcaaagac cttttttctg aacaaatgac ggaaacggat atcagccaat tcactatttt
    41821 tacggcttat ccagaatatg cattaacgac tgattggtac aaagaacaag ataaatcgca
    41881 gctgatccat catttgatcg atcttttacg gtttacgaca gtcaaatata cccggtcaaa
    41941 agttcgaaaa ggattgccga aagaatatag ctatatcatt gaggaactct tgtatatcga
    42001 tgaccgtatc aatggaaaaa aagactatgt aaaaaaaatc attgaacaac ttgttttgat
    42061 gaaggaagaa gaaaaatttt taacgaaact tgcacagacg atccaaaaat ctgtcatcga
    42121 tcatttgcat atcgttggag atatttttga ccgaggaact caagcagcca aagtgatgga
    42181 tcgaatcatg gatttttctt ctgtagatat ccaatgggga aatcatgata ttttgtggat
    42241 tggtgcgtat tgcggctctg aggcatgttt actgacactt ttacggattg cagcacgcta
    42301 taattattta tttgaattag agaaagaata tgggttgaat ttacgtccat tgttttcttt
    42361 tgctcatcaa acttatcaga agaatccagt atttgcgcct aaaaacagta aaaatctatc
    42421 cgaaagagag caagaagaat tagaaaaaat ccatcaggca ctttctatcc ttcaattcaa
    42481 atcagaacat cagttattgg atcgtcgacc tgaatttaaa atggacgatc gtaaattgct
    42541 ggaaaaaatc gactatgaag catcaacgat tgatttagaa ggtcagctct atgagttgaa
    42601 aggaacttgt tttcaaacga tccagccaga tgatccaaaa aaattcttgg aagaggaaca
    42661 aaaagtgatt gatagtttga tgtattcatt ccagcactct ttgagaatgc aaaaacatat
    42721 gacgtttttg atcgaaaaag ggggaatgta tctcacgaac aatcagcatg tgctgtttca
    42781 tggctgtata cctgtagacg agaagggaca gctcttagca tttgaattag accaatctta
    42841 tcgcggaaaa gaactgctgg gcttttttga aaaacagata actgaaagcg caaaagatct
    42901 gactgcaaaa gaagatttag caaccgattt gatttggtat gcgtggagtg ggccattttc
    42961 gcctttattc ggcaagagta aaatggcgac ttttgaacgt tattttctca cagagcctca
    43021 tacgcacgtc gagaaaagca atccttatta tcacttgcga gatgaagagt ggttcagtga
    43081 aaaaatttta gcagaattcg acacaaaaga agaagggtca gcaatcatta atgggcacac
    43141 gccagtaaaa gtaaaaaaag gggagtcacc gatcaaagca aatggaaaag tatttgtcat
    43201 tgacggaggt ttatccaaag cttatcaaaa aacgacagga attgccggtt attcactatt
    43261 atgcaattct tatggtttcc aaatcgtgac acattatccg tttttgccta ttgaacaaca
    43321 atttgcaaaa aaatccgacc aaacaaatgt gaaaaaagta atcgaacagc aactaccacg
    43381 caaactcaat cgtgatacaa caaaaggtat ggaactgcag cagcagatta cccagctcaa
    43441 aaggctgttg gactatcgaa aggacgattg agaagaattc cagtgaacat tgttccatag
    43501 actggagact gagacaaaag tcgtgtcgca gtctcttttt ccggataaac ggtgttcttc
    43561 tgccaactcc tcggtcacaa gtcacactat tcaacaaaca tgaaaagctg ttttttgaaa
    43621 ttgctccttg tgactcagag ttgaacggca ggctcacgac ctcttggtat aaacggtgtt
    43681 caaaagccag tttctcggta ttaagccaaa tttatgcaaa aatgtgaaaa gccattttca
    43741 caaaattttt cttaatactc gaaactaaac agctttttca caaccccctc agaaagtcta
    43801 ttaaaattca ttcacaaaga aatcgaataa gcgctgttca gatggatgta cacggtgggt
    43861 aagttccgga tgccattgaa caccgaggat tttatgcata tcatctttgc tttgaaccgc
    43921 ttcaatgagt ccatcgttag aaaaagcgat ggcttttaat aaaggtgaaa gatctttgac
    43981 tgcctgatga tggtaagaat tcacaaaata tggatcgggg agaaggttgc ttaacaaact
    44041 gtctgaaaca atcttgactt cgtgagtcgt aaattgcggg gcagtgggct gttgttcatg
    44101 ttttactgac cactttggat ataatgaaag atcttgataa agtgtacctt ccaaaacgac
    44161 attcaatagt tggagtcccc ggcagactgc aaaaattggt tttctttgtt tcaatgcttc
    44221 caagatcagt gcctgttcaa attggtctcg ttgaatattt gtttccgtta gctttggatg
    44281 aggatcttcc atgtaaagtt gtggactgac atcttgacca cctgcaagaa taagtttgtc
    44341 gatttgtcca atcagttgag gagctgaatc tggagacatg actggcagaa taagaggcaa
    44401 tccttgagca tctaaaactg catccacgaa tccttgcggt gtatagctca cttgatttcc
    44461 ttggaaagta tctgttgctc gaatcagttg atttccggca atgccgatga ttggtttcat
    44521 ttgtattccc cctaaagtag acttattgta acaaggattt aaaaaaaatt aaactatgta
    44581 agacttgccc ctatacttat ctgttcttgc tgtgttacta tctgtaaaga gaaatttcgt
    44641 gtgaaattca tgacgatagt tgaaaatatt gcttaatttg ttataataaa aagtaagcag
    44701 aaataaaaag gttgggcgta agcttcaagg atctagttct tcaaggggtg agaaggacat
    44761 tttattgaag gggcctgcct atactaagtt atctttagag gtacatactt tcaatgaaaa
    44821 aagaaagcat gtacctcttt tttaaatcga aaggaagtga agcaagtgtt agaagctttg
    44881 gaacaaatcc gaaaagcaga agaagaaaat gaaaagcgtg ctcaaacact gcggcagcag
    44941 ctgaagatct atgcaacaga aaaagagcag gaattggaaa cgatccaaac aaactatcga
    45001 caacaactaa aacagttggt ggaagaaaaa gaacaatttg aaatacaaga attagaaaaa
    45061 gaaaaaatga aattgaaagc agaagcagac aaggtgcaag caactttgaa agatcaatat
    45121 caaaagcatc agagtcaagc gatcggtgcg atcattgaaa gggtgaaaga aacttatggc
    45181 cgtcactaag atggaaaaag tgaccctcat ttcagataaa agaaatcagg agaggatttt
    45241 acaagcagta caaggaatcc aaaacgttga aatccgggat ctgtatcaaa atagaacaaa
    45301 caaccagtgg gtggagaagt attttccaga taccacgatt attgataaag aagcaaaatt
    45361 aagcgcttta ggtaatcggt tgaaagacat ccaagaagct atccaattca tacaacacca
    45421 cggcgatagc cgtcaaaaga aactccattt gaaacgtcaa gaactttcct tacaagattt
    45481 ggaagccaat tattccgaag aaaatttcat aaataaattg aaagaagtcc ttgatttaaa
    45541 agaaaagtgg gaacaactaa ctgaagaacg tgatcgttta gaagaagagg aagaatggct
    45601 tcttcattgg caacagctgg atattgctcc aagtatgtat gcaagtaaga cgaccatttt
    45661 tgagatgggg actgtgagtg cagcaaattt tgaaagtttc aaggaagaac tgagtaagct
    45721 tgatgacgtt tatatggaag aaatagatta tacgccaaaa caagtttatc tttcgtatgt
    45781 tgctttaaaa aaatcagttg ctgttataga agaaattgct gggaaatacg ggttcaataa
    45841 agaaacgtat ccttatgaac aaaatcctaa agaaaaactg caagagacca agcagcaatt
    45901 atccattgtt cacgagcagc aaaagaagat aacgacagcg ttaggtcagt gcagtggcta
    45961 tatccaagaa tttgaatggg tagaagaagt cacacttgcc gttgcagaac gagaaaaaat
    46021 aaaagaacgg tttatccatg cgacttatct gatcgttttg caaggttggg tagatgcaga
    46081 agaaaaacag aatttgctgc atgtgttaaa cgaaaccgta tcagaagaag atatatacgt
    46141 cagtttcgaa acaccaaact cagaagagat tcagcaggaa gtccctacta aattgaaaaa
    46201 ccatccgctt gtcgaacctt ttgaattgct gactgaaatg tacagtctgc caaaatatga
    46261 agaagtagat ccaacacctt ggatgacacc attttatctt gtcttcttcg ggatgatggt
    46321 cgcagatgtt ggttatggtt tgctgatgct cattgggaca atactagctc aaaaactact
    46381 tgttctacca cgcgggatga aacgtttcgt aaaattcttt gagattctct cgataccatc
    46441 gatcatctgg ggattgatct acagttcatt tttcggacag tcattaccaa aagaactgtt
    46501 tggcatccgc ttgcctttcc caatcctatc tacgacggat gacgtcaata cgattttgat
    46561 tttatccgtg attttcggtc tgatacagat tttggttgga ttatttgtgg cagcaaagga
    46621 aaatatcagg agaaaagatt acttgagtgc tgtcagtgat ggatttgcct ggcaagggat
    46681 cctaattggg atcgttattg cgttggttgg tgccatgttg ataaaaaata cagcatttgt
    46741 ttaccttggc gctgggctat cgatcattgc tgccttatgt atcttgatcg ttccgattat
    46801 ccaaacgccg tctaaagtca aaggtgctgc caaaggtgcg tataatcttt acggactgac
    46861 cggctatatc ggtgatttag tcagttatac acgtttgatg gcattgggta tttcaggcgg
    46921 aagtattgga gcggcattca atatgctagt tgctttcatg ccgccagctg ctcgattcag
    46981 tgtagggatc cttttgatca tcgctttaca cgcactgaat atgtttttga cactgctaag
    47041 tgcctatgtc catggcgcac gattgcagta tgtcgaattt tttgggaagt tttatacagg
    47101 aggcggacgt gcctttgatc cgctgaaaac ggcggaaaaa tacgtcaaca tcaatcacaa
    47161 gaaaaaaaac aataaataat tggaggattt ttatataatg atggattact taattagtca
    47221 aaatggtgga atggtatttg cggtattagc gatggcaaca gcgacgattt tttcagggat
    47281 cggttcagct aaaggtgttg gaatgacagg tgaggcagca gcagcattga caacaagtca
    47341 acccgaaaaa ttcgggcagg ctctgattct gcaattgctt ccaggtacgc aaggtttgta
    47401 tggattcgtt attgcctttt tgatttttat caacttagga aatgacatgt cagttgtaca
    47461 aggactgaac tttctaggtg cttctctgcc gattgctttt acaggcttgt tttcaggtat
    47521 tgcccaagga aaagtcgcag cggcaggtat tcaaatttta gcgaaaaaac ctgaacatgc
    47581 tacaaaagga atcatttttg ctgcgatggt tgaaacatac gcgattttag gtttcgttat
    47641 ttccttccta ttagtgttga atgcctaagc ataaaggagg aaaagcagat gaacgccatt
    47701 gaaaaaatca tctctcagat gaacgaagca gcagagcagg aaagagcggc gttagaacaa
    47761 gaagagcgaa tgaagatcga tcaaaacttt gaacaaaaac gtacgcaagt tgaaacagaa
    47821 catcaaaaac aaaaagaaaa acaaatcgaa ttactcgaaa aaaaataccg tcagctaagg
    47881 aatcgccagc aagtagaagt acgacaagaa aatttgaatg cgaagcagga atttttaaga
    47941 cgattgtttg cagatgcggt aactgaaatg gaaaattggg atgaatctga acaaatccag
    48001 tttatcaaaa atgcattgta ttcattacct ttgacaggaa aagtagcatt cattgctgga
    48061 gaaaaatcag cagcctatct ttctcagacg ttgttggatg aatggaacaa tgaacttccc
    48121 tttatgatgg ttttaagtga tgaaactgtt gctgatcaag cgggttttct gataaacgat
    48181 caaggagttc agtataattt tcttttcagc agccttgtac aagatatcca aggaacgatg
    48241 agttttgaga ttgccaatca attattcgaa taaggaggga tcagtgtatg gaataccacg
    48301 aactaaatcc cttgattcgc ggaagagagc ttgaactttt gtccaatgag acatttgaac
    48361 ggatgattca aacaaattct gtcgaagcac ttggtgaaat attgaaatcc acgatttaca
    48421 gtccatatat ttatgacgga tatgaatatg acttcgaaca gaatctttta aaggagcaga
    48481 gccgtctatt tcgctggtta aaggaatcag caccggagcc tgaaatcgtt tggatctata
    48541 cgatgcgtta tactttccat aatctgaagg ttttgacaaa agctgagatg acaaatcaaa
    48601 atctggacca tttgtatatc gacgatggat tttattcatt agaaaccttg aaggatgcca
    48661 ttcatacgca agtttcaaat gaacttcctg aaagcattat ggacagtata cgagaagtac
    48721 gtgattattt tgaagagtct accattcttc agggaattga cgtgatttat gatcgacagt
    48781 ttttgacaga gcagcgacgg cttgccgata agctaggata tcaagaattg ctgacagaag
    48841 tcattgcttt tattgacttg accaatatca caacgatggc tcgaggaata ttgcagaaaa
    48901 gaacatatgg gttcatgacg acggtacttt ccagctcggg aagtatcgaa aaagaagtat
    48961 ttttgaactt tgtagataaa gatatggaag attacacgaa ttttctatta gagacagatt
    49021 atgctgattt tctgcgccct gtcatccgtg agggaaatat tgatctcgta aagcttgaac
    49081 agttgaaaga tgattatcta agttcattgt atcaagaagc acaaacccag gctttcggac
    49141 cactgccact actagccttt ttgaacgcaa aagaggtaga aagcaaaaac cttcgtttac
    49201 tggttatcgg caaaagaagc catttcgaat cagaaacaat cagaaagcgg gtgagacaag
    49261 tctatgacgc ataagatcgg tgtagttggc gacaaagatt ctgttttgcc attcaaattg
    49321 tttggctttg atgttcgtta tggtacaacc aagcaaaccg ttcgtcaggc aattgaagaa
    49381 atggcaaaga atgagtatgg cgtgatttat gtcacagagc aatgtgcagc attgagccca
    49441 gaaacgatcg aacgttacaa ggaacaaatg atacctgcta tcgtattgat tcccaatcat
    49501 caaggatcat tagggatagg gctggaagaa attcaaaaaa acgtagaaaa agcagtaggt
    49561 caaaatatct tatagtatga aaaggagata gaaattttgc aaattggaaa aatcataaaa
    49621 gtctccggac ctcttgtaat ggctgagaac atgtctgatg ccagtatcca agacatgtgt
    49681 ctagtgggag atctaggagt tatcggagaa attatcgaga tgcgtggcga tgtggcgtct
    49741 attcaagtat atgaagagac ttctggaatt ggtccaggcg aaccggttcg ctctactggt
    49801 gaagctttat ctgttgaatt aggaccaggg atcatttcac aaatgtttga cggaatccaa
    49861 cggccattag atacctttat ggaaatcaca caaagtaatt tcttagggcg cggagttcaa
    49921 ttgcccgcat tggaccatga gaaaaaatgg tggttcgaac caacagtaga agccggagaa
    49981 acggtgagtg ctggagatgt tatcgggatc gtagaagaaa caaaagttat aaaacataaa
    50041 atcatggtgc caaatgggat aaaaggaacg atcaaaacaa tcgaaaaagg ttcattcact
    50101 attgatgaaa cagtatgtgt cattgaaacc gaaaatggtg acaaaaattt gacgatgctt
    50161 caaaaatggc cagtaagacg tagtcggcca atcaaagaaa aattaaaccc ggatgcaccc
    50221 atgatcactg gacagcgagt gattgataca ttcttcccag tgacaaaagg aggagcagca
    50281 gctgtacctg gtccttttgg tgcagggaaa acagtcgttc agcatcaaat tgcaaaatgg
    50341 gccgatgtgg acatggttgt ctatgtggga tgtggcgagc gtggaaatga gatgacggat
    50401 gttttaaatg aattccctga attgattgac cctaatacag gagaatcgtt gatggaacgt
    50461 acggtattga ttgctaatac atcaaacatg ccagtagcag ctcgagaagc ttctatctat
    50521 acgggaatca caattgcgga atatttccgt gacatgggtt atgacgttgc tatcatggca
    50581 gactccactt cacgatgggc agaagcactt cgtgagatga gcggacgttt ggaagaaatg
    50641 cctggtgatg aaggttatcc agcttatctt ggttcacggc ttgcggaata ctatgaacga
    50701 tctggaagag tcattgcatt aggatcagaa ggtcgcgaag gaagtataac cgcaatcagt
    50761 gctgtatccc cgtctggagg ggatatttcg gaaccagtca cacaaaatac attgcgggtc
    50821 gtaaaagtat tctggggtct tgattccagt ttagcccaaa aaagacattt tccttcgatc
    50881 gattggatac aaagctattc tctttattca acagaagtag ggaagtatat ggatcatatc
    50941 cttcaagctg actgggcatc catggtcacc gagggcatgc gtattttgca ggaagaacag
    51001 cagctgaatg aaatcgtgcg tttggtaggg attgattccc tttcagataa tgaccgtctg
    51061 acattagaag tggcaaaatc cattcgtgaa gattatttgc agcaaaatgc ttttgatgat
    51121 gtcgatacat tcacttcaag agagaaacaa ttcaaaatgt tgaagatgat cttgaccttt
    51181 ggtgaagaag gacgccgtgc gttgtcgtta ggtgcttatt taagtgagat catggacggt
    51241 accgtatcga ttcgtgaaag gatcggacga agcaaatata tcccggaaac agaattagaa
    51301 aaactagatc aaattattgg tgaaatcaag caaacgatgc aaacgatcgt ttcagaagga
    51361 ggcatgagca atgattaaag aatatcgtac gatcggtgaa gtcgtcggtc cattgatggc
    51421 ggttgaaaaa gttgctggtg tcaaatatga agaattaatc gaagtccgta tgcaaaatgg
    51481 cgagattcgt cgtggacaag tattggaagt ccaagaagat aaagcaatgg tccaaatatt
    51541 cgaaggaaca agtggtatca atttacgtga ctcttctgta cgatttttag gccatccttt
    51601 agaactaggt gtatcggaag acatgatcgg tcgagtattc gatggtttgg gacgaccgaa
    51661 agataacgga ccagagatcc tgcctgaaaa gcatttggat attaacggag aagtaatcaa
    51721 tccagtagct cgggattatc cagatgagtt tatccaaaca ggtatctctg caatcgatca
    51781 tttgaataca ttagttcgtg ggcaaaaatt accagtattt tctggctctg gtcttcctca
    51841 taaagaatta gctgctcaga tcgcgcgaca agcaacggta ctcgattcat cagatgattt
    51901 cgccgtagtc tttgctgcta ttggaatcac ttttgaagaa gctgaatttt tcatggaaga
    51961 ttttcgtcaa acgggcgcaa tcgaccgttc tgtcatgttt atgaatttag caaacgaccc
    52021 tgcaatcgaa cgtatcgcta ctcctcgtat ggctttgact gctgcagaat atcttgctta
    52081 tgaaaaggga atgcatgttt tggttatcat gacagacatg accaactatg cagaggcatt
    52141 gcgagagatt tctgcagcac ggcgagaagt tccaggaaga agaggatatc ctgggtatct
    52201 ttacacgaac ttagctactt tattcgaacg agcaggaaga atccgcggac taaaaggatc
    52261 ggttactcaa attccgattc taacaatgcc agaagacgat aaaactcatc caatccctga
    52321 cttgactggt tatattacag aaggacagat catcttgact agagagcttt acaagagtgg
    52381 gattcagcca ccaattgatg tactcccgtc tctttcgcgg ttaaaagata agggaactgg
    52441 acccggaaag acaagagaag accatgcagc tacgatgaat cagctttttg cagcctatgc
    52501 acaaggaaaa caggcaaaag aattagccgt agttttaggt gaatctgctt tatctgatgt
    52561 agacaagatc tatgcgaagt ttgctgagcg gtttgaaaaa gaatatgtca atcaaggatt
    52621 ctacacgaac cgttcgatta ctgaaacact tgatctaggg tgggaactgc tatcgatgtt
    52681 gccaagaacc gaattaaaac gaatcaaaga cgacatgctg gacaaatatc tgccagaagg
    52741 gaagtgagtt ctgatggcac gattgaatgt gaatccgacc cgcatggaat tgactcgatt
    52801 gaaaaaacag ttgacaactg ccacaagagg gcataaatta ttaaaagata agcaagatga
    52861 actgatgcgt caattcatcc tgttgattcg aaagaacaac cagctgcgtc aagaaatgga
    52921 agagtctatg acagaagcaa tgtctgattt tgtattggca aatgcttcga tcacggaacc
    52981 tttcatcgaa gaattatttg ttttgccggc ggaaaacgtt gatttgtcta tcgtagacaa
    53041 aaatatcatg agtgtaaaag tacctgtgat gaattttgaa tatgatgaac agctgacaga
    53101 cacacctttg gaatacgggt atttgaattc cagtgccgaa ctggatcggt caatcgatcg
    53161 gtttactacc ctgcttccaa aacttttgga actgacagaa atcgaaaaaa cttgtcaact
    53221 gatggcgagt gaaatcgaaa agacacgtcg gcgagtcaat gcattagaat atatgacgat
    53281 ccctcaatta gaagaaacga tctattatat tcgtatgaag ctggaagaaa acgagcgcgc
    53341 agaagtaaca aggttgatca aagtaaaaaa tatgggtacc actgactaag tggtatcttt
    53401 ttttaaaaga ggaaaacgat gattaaacaa agcaaacaaa taaagaaacg tttgccggct
    53461 gttcagcttc tcgcattagg attttttctc ctgattttag ttggcggcgg cctacttacc
    53521 ctaccatttt ttagtaatag tgggcatggg acaaatttta tcgatgcgct tttcactgca
    53581 acttctgctg tctgtgtaac gggattgaca acattgaata cagcagagca ttggaacagt
    53641 gctggtcaat ttttgatcat gcttttgatt gagatcggcg gtcttggttt tatgatggtt
    53701 ccgattctat gctttgcatt gataaagaag aaagtcagtc tcagcacacg tattatatta
    53761 aaagaagcgc tgaatctaga agaagtatca ggtgtgatga agctcatgct ttacatccta
    53821 aaattggcct ttgtgatcca attgattggt acggttgctt tatcttttgt atttgtgcct
    53881 gaatttggct ggttgaaagg tatctggtac agtttgttcc atgcaatctc tagtttttgt
    53941 aatgcagggt ttgatttgtt aggagacagt ttagccaatc accagcaaaa tgtttatttg
    54001 atcatggtgg tatctgcttt gatcatttct ggtgggttag gcttcatcgt atggcgtgat
    54061 ttactggaat atcatcagaa aaagaagatg acgattcatt caaaaatcgc attaaccgtt
    54121 acaggtaccc ttttattagg cggattcgtt gtgttctttt tcacagagcg caatggggca
    54181 acgctagtgg aagggaatat gtttcaacga ttggcaaaca ccttttttat gagtgtgaca
    54241 cctagaacgg ctggatatta ttcagttgat tacctccata tgagccatgc aggtctgatt
    54301 ttaacgatgt ttttgatgta catcggcgga acatctgggt cgacggccgg cggattgaaa
    54361 acaactacgt taggtgtgtt gctgatccag atgcgttcga tgttgaaagg acgtacgaga
    54421 gcggaagcat ttggccgtac catccgacaa ggcgcagtgt tgcgggcact tactttattt
    54481 tttgttacgt tgacgttgtg tgtagtcgcc atcatgattt tatcggtgac tgaaacgata
    54541 cctaaaactt ctggcatcga atatattgct tttgaggtct tctctgcgtt tggtacagta
    54601 ggtcttacga tgggattgac gccagacttg actgcaatag ggaaattgat cattatagcg
    54661 cttatgtaca ttggacgagt tggtgtattg actgtggtct tctctctgat gacaaaagca
    54721 caaagaacac aatcaaaata caaatatcca gaagaaagtg tcatgattgg ataatttcat
    54781 cttctgacag aaacctagtg atttagtaaa ataagaaaat aaaataattt ttcttgcttc
    54841 ttggatactt taagtgcaaa atgttc
//
LOCUS       DANKKG010000006        53645 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000006 DANKKG010000000
VERSION     DANKKG010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53645
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.006"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            20..1651
                     /locus_tag="PW004_000282"
     CDS             20..1651
                     /locus_tag="PW004_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HDL2725282.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(1713..2354)
                     /locus_tag="PW004_000283"
     CDS             complement(1713..2354)
                     /locus_tag="PW004_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDL2725283.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    2540..2616
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            2636..2944
                     /gene="rplU"
                     /locus_tag="PW004_000284"
     CDS             2636..2944
                     /gene="rplU"
                     /locus_tag="PW004_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HDL2725284.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            2971..3315
                     /locus_tag="PW004_000285"
     CDS             2971..3315
                     /locus_tag="PW004_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HDL2725285.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            3328..3621
                     /gene="rpmA"
                     /locus_tag="PW004_000286"
     CDS             3328..3621
                     /gene="rpmA"
                     /locus_tag="PW004_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HDL2725286.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            4055..6862
                     /locus_tag="PW004_000287"
     CDS             4055..6862
                     /locus_tag="PW004_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL2725287.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            7053..7544
                     /locus_tag="PW004_000288"
     CDS             7053..7544
                     /locus_tag="PW004_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2725288.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            7647..8636
                     /gene="manX"
                     /locus_tag="PW004_000289"
     CDS             7647..8636
                     /gene="manX"
                     /locus_tag="PW004_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HDL2725289.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            8669..9472
                     /locus_tag="PW004_000290"
     CDS             8669..9472
                     /locus_tag="PW004_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HDL2725290.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            9493..10407
                     /locus_tag="PW004_000291"
     CDS             9493..10407
                     /locus_tag="PW004_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HDL2725291.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            10490..10864
                     /locus_tag="PW004_000292"
     CDS             10490..10864
                     /locus_tag="PW004_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HDL2725292.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            11003..11248
                     /locus_tag="PW004_000293"
     CDS             11003..11248
                     /locus_tag="PW004_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDL2725293.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            11420..12484
                     /locus_tag="PW004_000294"
     CDS             11420..12484
                     /locus_tag="PW004_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDL2725294.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            12730..13155
                     /locus_tag="PW004_000295"
     CDS             12730..13155
                     /locus_tag="PW004_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDL2725295.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            13148..13597
                     /gene="nusB"
                     /locus_tag="PW004_000296"
     CDS             13148..13597
                     /gene="nusB"
                     /locus_tag="PW004_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HDL2725296.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            13694..14548
                     /locus_tag="PW004_000297"
     CDS             13694..14548
                     /locus_tag="PW004_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HDL2725297.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            14549..15886
                     /locus_tag="PW004_000298"
     CDS             14549..15886
                     /locus_tag="PW004_000298"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HDL2725298.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            15891..16115
                     /locus_tag="PW004_000299"
     CDS             15891..16115
                     /locus_tag="PW004_000299"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HDL2725299.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            16117..17007
                     /locus_tag="PW004_000300"
     CDS             16117..17007
                     /locus_tag="PW004_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDL2725300.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            17016..17834
                     /locus_tag="PW004_000301"
     CDS             17016..17834
                     /locus_tag="PW004_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HDL2725301.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            17941..18390
                     /locus_tag="PW004_000302"
     CDS             17941..18390
                     /locus_tag="PW004_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HDL2725302.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            18408..20090
                     /gene="recN"
                     /locus_tag="PW004_000303"
     CDS             18408..20090
                     /gene="recN"
                     /locus_tag="PW004_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HDL2725303.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(20145..20261)
                     /locus_tag="PW004_000304"
     CDS             complement(20145..20261)
                     /locus_tag="PW004_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HDL2725304.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(20334..21290)
                     /locus_tag="PW004_000305"
     CDS             complement(20334..21290)
                     /locus_tag="PW004_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDL2725305.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            21461..21829
                     /locus_tag="PW004_000306"
     CDS             21461..21829
                     /locus_tag="PW004_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HDL2725306.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            21999..22430
                     /gene="mraZ"
                     /locus_tag="PW004_000307"
     CDS             21999..22430
                     /gene="mraZ"
                     /locus_tag="PW004_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HDL2725307.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            22451..23410
                     /gene="rsmH"
                     /locus_tag="PW004_000308"
     CDS             22451..23410
                     /gene="rsmH"
                     /locus_tag="PW004_000308"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HDL2725308.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            23415..23816
                     /gene="ftsL"
                     /locus_tag="PW004_000309"
     CDS             23415..23816
                     /gene="ftsL"
                     /locus_tag="PW004_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HDL2725309.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            23817..26009
                     /locus_tag="PW004_000310"
     CDS             23817..26009
                     /locus_tag="PW004_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDL2725310.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            26035..26997
                     /locus_tag="PW004_000311"
     CDS             26035..26997
                     /locus_tag="PW004_000311"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HDL2725311.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            27011..28372
                     /locus_tag="PW004_000312"
     CDS             27011..28372
                     /locus_tag="PW004_000312"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HDL2725312.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            28398..29486
                     /gene="murG"
                     /locus_tag="PW004_000313"
     CDS             28398..29486
                     /gene="murG"
                     /locus_tag="PW004_000313"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDL2725313.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            29501..30721
                     /locus_tag="PW004_000314"
     CDS             29501..30721
                     /locus_tag="PW004_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HDL2725314.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            30877..32202
                     /gene="ftsA"
                     /locus_tag="PW004_000315"
     CDS             30877..32202
                     /gene="ftsA"
                     /locus_tag="PW004_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HDL2725315.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            32225..33466
                     /gene="ftsZ"
                     /locus_tag="PW004_000316"
     CDS             32225..33466
                     /gene="ftsZ"
                     /locus_tag="PW004_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HDL2725316.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            33483..34160
                     /locus_tag="PW004_000317"
     CDS             33483..34160
                     /locus_tag="PW004_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HDL2725317.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            34174..34773
                     /locus_tag="PW004_000318"
     CDS             34174..34773
                     /locus_tag="PW004_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HDL2725318.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            34788..35075
                     /locus_tag="PW004_000319"
     CDS             34788..35075
                     /locus_tag="PW004_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HDL2725319.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            35124..35906
                     /locus_tag="PW004_000320"
     CDS             35124..35906
                     /locus_tag="PW004_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HDL2725320.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            35927..36634
                     /locus_tag="PW004_000321"
     CDS             35927..36634
                     /locus_tag="PW004_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HDL2725321.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            36896..39694
                     /gene="ileS"
                     /locus_tag="PW004_000322"
     CDS             36896..39694
                     /gene="ileS"
                     /locus_tag="PW004_000322"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HDL2725322.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(39743..41269)
                     /locus_tag="PW004_000323"
     CDS             complement(39743..41269)
                     /locus_tag="PW004_000323"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HDL2725323.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(41284..41931)
                     /locus_tag="PW004_000324"
     CDS             complement(41284..41931)
                     /locus_tag="PW004_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HDL2725324.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(42115..42444)
                     /locus_tag="PW004_000325"
     CDS             complement(42115..42444)
                     /locus_tag="PW004_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HDL2725325.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            42621..43349
                     /gene="sfsA"
                     /locus_tag="PW004_000326"
     CDS             42621..43349
                     /gene="sfsA"
                     /locus_tag="PW004_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HDL2725326.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            43365..44378
                     /locus_tag="PW004_000327"
     CDS             43365..44378
                     /locus_tag="PW004_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL2725327.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            44378..45655
                     /locus_tag="PW004_000328"
     CDS             44378..45655
                     /locus_tag="PW004_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDL2725328.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            45718..48420
                     /locus_tag="PW004_000329"
     CDS             45718..48420
                     /locus_tag="PW004_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL2725329.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            48572..48889
                     /locus_tag="PW004_000330"
     CDS             48572..48889
                     /locus_tag="PW004_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2725330.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            48919..49239
                     /locus_tag="PW004_000331"
     CDS             48919..49239
                     /locus_tag="PW004_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDL2725331.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            49347..50807
                     /locus_tag="PW004_000332"
     CDS             49347..50807
                     /locus_tag="PW004_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2725332.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            50875..51096
                     /locus_tag="PW004_000333"
     CDS             50875..51096
                     /locus_tag="PW004_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725333.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            51127..51309
                     /locus_tag="PW004_000334"
     CDS             51127..51309
                     /locus_tag="PW004_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HDL2725334.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            51309..51722
                     /locus_tag="PW004_000335"
     CDS             51309..51722
                     /locus_tag="PW004_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HDL2725335.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            51845..53026
                     /locus_tag="PW004_000336"
     CDS             51845..53026
                     /locus_tag="PW004_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2725336.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(53097..53261)
                     /locus_tag="PW004_000337"
     CDS             complement(53097..53261)
                     /locus_tag="PW004_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725337.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            53415..53488
                     /locus_tag="PW004_000338"
     tRNA            53415..53488
                     /locus_tag="PW004_000338"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:53449..53451,aa:Arg,seq:cct)
BASE COUNT        17708 a         9104 c        11607 g        15226 t
ORIGIN      
        1 tgaaaaagga gttagatagg tgttcaaaaa ttatgtttcc tcactaaaaa aagaattttc
       61 aggttatgat ctttcaagat ttcagaaaga tcttttggct gggatcacag tagctgctgt
      121 agcattgcct ttagccttgg cttttggggt atcaagcgga gctgatgcgg ccgcaggttt
      181 gattacggct atcattgccg gtatcgttat tggcggttta tcaggcggtt tttatcaaat
      241 atcaggtccg acaggtgcga tggcagctat cttgatgtct attgcggcaa caaagggaat
      301 gcaaggtgta ttgcttgcta cttttttagc gggtctattt ttacttgcag caggactttt
      361 ccgattagga acactgactt cttttatccc agcacccgta attacggggt ttacttcagg
      421 tattgctatc attattgcat taggtcagat cgataattta tttggtgttc actcggaagg
      481 tacaaacgtt attgaaaaac taagtagtta tcaaacgata ggatttccga taaacacagc
      541 ctctttgatc atgggagggc tagtcatttt agggatgttt atctatccaa aaaaatgggc
      601 gaaacgaatg cctagttcgc tgctggctat cattttggca acagcagtta tgctattgtt
      661 ccatttacca gtagcaactg tgggtaagat tccacaaaca ttgatcagta gtaatcgttt
      721 gaatatggga gattttagct tttctgcctt gcaacaagtg gcagttccag ctattagcat
      781 tgctttatta ggaatgattg aaagcttgct atgtggtgct tcggctggcc gaatggcaaa
      841 taagccctta gatagcaatc aagaattaat ggcgcaagga attggaaatc tgttgcttcc
      901 tttctttggc gggattcctg ctactgcggc catcgcccgt acaagcgtgg ccatcaaatc
      961 tggtgcacaa actagattag ctggtatgat acatgcagtt gtcttactgc tctccatgct
     1021 tgtttttgcg ccagtgatgt cacagattcc aatgccagca cttgcaggtg ttttgattgt
     1081 gacggcttgg cgtatgaatg aatgggaaac gatcaaggag ctatttacta ataaatattg
     1141 gtcagcagtt ctattgttta tccttacgat gatttgtaca gttatctttg atttaagtat
     1201 tgctatcgtg atcggggtga tcggcggctg tgtatttttt gttgtcaaaa gcgctgctat
     1261 cacgatttca ttagaaacga tcgattggca aagaatgggg ctgccagaaa cggaaaagct
     1321 gaataactgg acagtagtgt atattagtgg tccactgttt tttatgtctt cagaaaaact
     1381 caaaaataaa ttggaagaac taaatcatca cgatggtgtc attttctcca tgcgaggcgt
     1441 accaagtatt gatttgacgg cacgtgcagt ttttgatgaa tttcaagaaa aagcagagca
     1501 aagaggacaa acgattatct atacttcctt gcagccagaa gtagaaaaac aactttctca
     1561 cttgtgggaa catacattca cgcatcaaca tcaaacagtt gctcatgcgt tgacagcttt
     1621 acatgcgaaa tatactgcaa ttagccatta aaacaatgaa aaagaacaga caagactgta
     1681 aactgaagat tttatggtct tgtctgttct ttttaatatt gctgtaaaag tgaagctagt
     1741 acttgccagt cttgatggat aacaggtaca agggagcgat tgtagaaaat agctgctgga
     1801 tgaaaaagtg gaagaattcg atagtttttt tctgaccatt cgtaggcatc cttttgttct
     1861 gttaattgta agacaggccc tgtatagagt tgaccatgat ttttggtaac cgtatattct
     1921 ttgccaagta aacgttgaag accaatgttt ccaagtgtcg caattaattt tggctggacc
     1981 ttcatcagtt cgtaatccaa tattggtgca tgcgcaaata cttcggaacg agtaggtgtc
     2041 cgatttggat aaacagtttc tgactgttgt gtccgtttat tgatccgatg agtcacgcga
     2101 tacgggcggc tccttacagc actggtgata tacacctctt ctcgtgttaa gccagttgaa
     2161 gaaagtgcct gcatcaattc ttttccagct tgaccgctaa aaggaacatg cgtatcaatc
     2221 tctgttttcc ctggggcttc tcccacgagc atcagtttag gatgaagagg tccttgccca
     2281 gcgataaatc cttctacagg acggccgttc attcttaaag aagcttcttg tatcagttct
     2341 tttgggtatt ccatttatgc tcctcctttt tcttttatta taagaataaa agaaacacaa
     2401 tgcgactatt ttatttggta gaactgtgag gatagaaaag cagaagtatt gcttgaattt
     2461 ttgttttact ttttgatttt aaatttagtt gactaacggt tgctgttctg gtaagctagt
     2521 actgttgtaa ttgtggactc cacagctaca accgctcaga acaagtaata agcacagcat
     2581 atgctgtcac ttcggatggc gagtctaagt atatcagaag gaggtgcttt atagcatgta
     2641 cgcaattatt aaaactggtg gtaaacaagt aaaagttgaa gtgggacaag caatctatgt
     2701 tgaaaaactt gacgttgaag caggagaaaa agttgttttt gacgaagtta tcttagtagg
     2761 cggagaatct acaaaagtag gcgctccaac tgttgcaggt gcaactgttg aaggtactgt
     2821 tgaaaaacac ggcaaacaaa agaaagtcgt tactttcaaa tacaaaccta aaaaacattc
     2881 acaccgtaaa caaggtcatc gtcaaccata tacaaaagtg gttatcgacg cgatcaacgc
     2941 ttaatagcgt ttgagaaaga aggcctatat atgatcaaag gaacatttaa gcgaaatgac
     3001 gcaggtcaga tcgtttcatt cactttgacc gggcatgctg atgctggtcc ttatggaagt
     3061 gatatcgttt gtgcgggtgt ttctgcgtta gcgatcagca ctgtcaacgg gattgcctca
     3121 ttagccggtt ttgagccgat tgttgaaatg aatgaagaag aaggcggtta tctttatacc
     3181 gaagtcactt caggaatgac acaagaacaa aacaatattg ctgagattct attagaaaat
     3241 cttttattag gattacaatc gatagaagct gaaaactcag aatatatcca aattaaaaca
     3301 attaatgata aataggaggt gcagaccatg ttgttatcta tgaatctaca attatttgcc
     3361 cacaaaaaag gtggcggttc tacatctaac ggccgtgact cagaatcaaa acgtctaggt
     3421 gctaaacgtg ctgatggaca aacagttaca ggtggatcaa ttctttaccg tcaacgcggt
     3481 acaaaaatct atccaggtgt aaacgtgggt atcggtggag acgacacttt atttgcaaaa
     3541 gttgatggcg tagtacgttt cgaacgtaaa ggccgtaaca aaaaacaagt gtctgtttat
     3601 ccagtagctc aagaagctta attgaatctt atgattaaag aggttgtgac aagacttttg
     3661 tcacagcctt ttttgtttat aaaaataaaa gttgtagtgg aggctacgaa gtttactttg
     3721 tctttttaca cttaatattt ttttgccaat ttggaatcag gatgttttga taactatcgt
     3781 tttcaattga tggaagctat attgtttgct atcagtgaaa taattatatg gtaaacagtg
     3841 ttctttttct tgtttccatt ataaaaatcg tatcgggatt tttcgttctt ttaaattgaa
     3901 catttctatc tatccgcgtg ttatcaacaa aacaaaatca ggtaaatagt tgacgaaaca
     3961 gaaataaata catagtatta tgttttgaag cctaagtaca atagtctata gaaaaggagc
     4021 gataccattg gcaaaacgca ttgaccgaat ttatatgtat gtcaaagaaa aaaccgctca
     4081 tttaacaaag gcagaatatg atcaaggagt aacaacacag gaaattgctg aagtgttagg
     4141 gattcagcga acaaatagca gtaaagatct gaatcagcta gtgcgcgaag gaaaactgat
     4201 aaagacagac ggacgaccag tacgttacat atatcaaaca ctcgttacgc acgtaaagcc
     4261 agttacaaaa catgtagtca gttacaaaga aacgccggtc tctgaagaaa aaaatacact
     4321 ccaaatagat actaaggata tctttgcaaa aatcattgga gctaacggca gcatgaaaaa
     4381 ttcagtggaa caggcaaaag ctgcgatttt gtatccgcca agaggattaa attgtttgat
     4441 tacaggacct accggatcag gaaagactta ttttgcacac gcgatgtatc attttgcaaa
     4501 agcgcatcat gtaatagatg aggaaacgga actgattgtt ttcaattgtg ctgattatgc
     4561 caataaccct gagcttttga tgagtcattt atttgggtac gcaaaaggtg cttttacagg
     4621 cgcagatgaa gaaaaaatgg ggattatcga tcaagcggat ggcggtatgc tgtttttgga
     4681 tgagattcac cggttgccgc ctgaaggtca agaaatgatc ttttacttta tggatcatgg
     4741 aacttacagt cgtcttgggg aaacaacgaa atcccatgaa gcaaatgtac ggatcgtagg
     4801 tgcgaccaca gaagacccag gttcttctct attagaaact tttgttcggc gtatcccaat
     4861 caatatcaaa cttccggctt ttgaaaaacg tcctgcaaat gagaaagtcg atttagtaaa
     4921 aatcatgatt gcccacgaag caaaccgcac gcagcgaaag atttctttga cagaagatgt
     4981 agcaaaagca ttgatcggca gtgtgaccta tggaaatata ggacaattga agtcaaatat
     5041 ccagttagta tgtgcacgag gatttctaaa tcatatgcaa tcacccgaaa tctcgatcac
     5101 aatcgatgat ttgacagaag ggattcgtag cggattgatt caattagcaa gcaaccgcag
     5161 tgcaatggca gagctttcta aaatattgga gccaaagata actgtagcac caaatgatac
     5221 gatcatgaaa atccaatcgg actcctatga gttaccttat aatctatatg atatcatcgg
     5281 agataaagca gctttactga agtcggatgg tttggatcaa gaagcaatca atcatttcat
     5341 ttctacagat atcaacatcc atctgaagtc tttctataaa gaccatggtt tttcttttaa
     5401 tgcagacagc aaattagcag aatttgtaga tccgaaaatc atagaagtga ccaatcagat
     5461 ttatgtaatg gtcaaaagat ctttaccata tgagtttcag caaaacttta tttatgctat
     5521 gagcttgcat atcagttctt ttttgaaaaa aatcaactta ggagaagaac ggcatacgaa
     5581 tgataacatc cgtgagatgg cttttgattt tccagatgaa tatgctgttg cgaaagaagt
     5641 gcgtagttat atcgaaacgt attttcaagt ggagattcca gaaagtgaag actattactt
     5701 gaccgttcta ctcgtctcgt tacgggcaaa tcaagcaagc ggcagaatcg gtgtagtcgt
     5761 tgcagctcac ggaaacagca cagcaagcag tatggtccaa gttgtccaac agttgttaga
     5821 tgcagagaac gtacgagcag tagacatgcc tttggatatg gatccgaaaa cagcattgga
     5881 ccgaattgaa cgcaatgtac aagaagtaga tgaaggaagc ggggtcatac tcttagtaga
     5941 tatggggtcc cttgcgtcgt ttaattcgca aattcaaaga gacactggaa ttccagtacg
     6001 tactgttgat atggtaacaa cttctctagt tttggaaact gtacgaaagg tctcagtatt
     6061 agggacagat ctagatatgt tgcatgaatc attgaaaaat tttcgaggtt acgcagaaat
     6121 atcttctgta gaatctgttc aggaagcaaa acctattcat aagaaaaaag ctgttttggc
     6181 tatctgcgca tctggcgaag gtacagcaca aagaatcaaa gaattgatcg aacgagcggt
     6241 aaacaagcgt caagaaacag aattagaagt tctagctttg tcgattgtgg aagtgaaaga
     6301 agaactgcca gttatccaaa aagaatatca gatcatagct actactggga ttactgattt
     6361 gaatatccaa gccccattta ttcctttaga acgttttatt gatcaaaata tcgaagtcat
     6421 tttggatcag ttgctgatgg aatcagaatt ggaggaaact gaattcatct ccctagatga
     6481 agagtcagct aaaaatacgt gtgttgagtt tatctctgat aacttcatct ttattaatgg
     6541 tagtaagtta atcgatccaa tgtggcaatt ttctacacaa atctctcaga cgacgggaat
     6601 cggcgatgaa gaatatggat tcaaaatcaa cctagtgatg cacacagcag gaatgatcga
     6661 acgtattatt cggaatgaac cattgactgt ggaagaaaac gaattgacaa atacaacgaa
     6721 tgatcctctt tatagccaat tggcagcctc agttgttctg ttggaagatc agataaaagt
     6781 aaaagtgccg atagaagaaa tgtattatct gctacgtcta gtccataatc agttagataa
     6841 aaaagaatac actgtgccat gaatacacaa aaacgataca cttttatagt gtatcgtttt
     6901 tgtgtattgg ttaagaatcc tttaaattca attagtgtat cgtaaaaaag ttggcacgct
     6961 ttttgcttat agtagtgtgt gctaggaggt ggttgttcac acagaaatga taggggtttc
     7021 tgtgaagtag tgaggaagaa ggagttttgc agatggatat tcgattggca cgtatagatg
     7081 atcgtttaat ccacgggcaa gtcgcaacag tttggtcgaa gcaaacaggt gttgaacgta
     7141 tcattgtgat cagcgatgta gtcgctcaag ataaattgag aaagtttcta ctaaaagaag
     7201 ctgcaccgcc ggggatcaca gcaaatgtaa taaccgtgca gaaaatgatt caaatctatc
     7261 atagtggatt attatcacga gcaaaagtga tgcttttatt caccaatccc caagatgttg
     7321 aagcggttgt ccgctgcggt gtacagttga agtcgttgaa tatcggcggt atgagtttta
     7381 ctgaaggcaa atcaatgatt accaattttg tttcagtaaa tcaacaagat attgattcct
     7441 ttcgttactt gaacagtcaa gaagtggagt tagagattcg aaaagtacct gctgacagga
     7501 aaatcctttt gatggaacta ctagcgaaaa tcaaaaaggt gtagcaagaa tggttgttgg
     7561 aaaaggtcaa ataattctgt tgtaatggtt agcaattatt aatatttttg aatttgttac
     7621 aacaaaagta aataggaggt ataaatatgg tagggattat cctagcaagt catggacaat
     7681 ttgctgaagg aatcttacaa tccggttcaa tgatctttgg cgaacaagaa aacgtcaaag
     7741 ccgtgatttt gaaaccaagt gaaggtccag atgacttgag agccaaattg gaagaagcgg
     7801 ttgcttcttt tgataatcaa gacgaagtgt tattcttagt tgatttatgg ggcggaacac
     7861 cgttcaatca atcaaacaca ttattcgaag agcacaaaga taaatgggca atcgtcagcg
     7921 gtttgaattt accaatgttg attgaagcct acgcatcacg cttttcaatg gaatctgctc
     7981 atgaaattgc agcacacatc attgaaacag caaaagacgg tgtaaaagta aaaccggaag
     8041 aattagaacc ggcagaagca ccgaaagcag cagttgaaga cgcacaacct aaaggagcac
     8101 ttccagaagg aactgtggtc ggagacggaa aaatcaaata tgtattagca cgtgttgact
     8161 cacgtctgtt acatggacaa gttgcgacag cttggacaaa agcagtgcaa ccaaaccgta
     8221 ttatcgttgt ttcagatgca gtatcaaagg atgatctgcg taagagacta atcgaacaag
     8281 ctgctcctcc aggtgtaaaa gcaaatgtta ttcctattag caaaatgatc gaagtagcaa
     8341 aagaccctcg ttttggaaat accaaagcat tgcttttatt tgaaaatcct gaagacgtat
     8401 tgacagctgt tgaaggtggc gtagacatca aagaattaaa tgttgggtca atggcgcatt
     8461 cggtcggtaa agtcgttgta agtaaagtat tgtctatggg acaagaagac gttgaagcgt
     8521 ttgagaagct tgaacaaaaa ggtgtgaaat tcgatgtacg taaagtacct aatgattcac
     8581 gcgataacat ggatgatatt ttgaaaaaag caaaagctga attagcaaaa gcgtaataaa
     8641 taatattaaa atttatagga ggcttatcat gtctattatt tcaataattt tagtcgtatt
     8701 agttgccttt ctagctggta tggaaggtat tcttgatgaa tttcaatttc atcaaccact
     8761 agtggcatgt acattgatcg gtttagttac cggtaacctc gaagctggta tcgtacttgg
     8821 cggtacgctt cagatgatcg ctttaggttg ggcaaatatc ggtgccgctg tagcaccaga
     8881 tgctgcatta gcatcagtag catcagcaat tattttagtt ttaggtggac aaggtgttaa
     8941 aggtgttcct tctgcgatcg cgattgccgt tcctttggct gttgcagggt tgttcttaac
     9001 aatgatcgta cgtacaatcg ctgttccaat cgttcatttg atggatgcgg ctgctgaaga
     9061 aggaaacatc cgtaaggttg aaatgtggca catcattgct gtatgtttac aaggggttcg
     9121 tatcgcggtc ccagcagcag cgttgctatt tatcccagca caaactgtgc aatcattcct
     9181 tgaatcaatg ccagcatggt taacagatgg tatggctatc ggtgggggaa tggtcgtagc
     9241 tgtaggttac gctttggtaa tcaacatgat ggctacaaaa gaagtatggc cattcttcgt
     9301 tattggtttc gttgtagctg caatttctca attaacattg attgctctag gtgctttagg
     9361 tgttgcatta gctcttattt acttgaacct ttctaaaatg ggtggttcat caaataatgg
     9421 aggcggaagc aactctggtg accctttagg cgacattttg aatgactatt aatcttgaag
     9481 gaggaaaata aaatggcaga agaaaaaatc aaattatcaa aaaaagatcg tttagctgtt
     9541 gcgtggcgct ctaccttcat tcaaggttca tggaactacg aacgtatgca aaacggtggc
     9601 tgggcatttt ctatgattcc agcaatcaaa aaattataca aaacaaaaga agaacgttct
     9661 gcagcattaa aacgtcactt ggaattcttt aatacacacc catacattgc ttctccaatt
     9721 ttaggggtta cattggctct ggaagaagaa cgtgcgaatg gcgcgcctgt tgatgatgta
     9781 gcgattcaag gggttaaagt aggtatgatg ggtcctttag ctggtgttgg tgacccagtg
     9841 ttctggttta cagtacgtcc aatgctaggt gcactaggtg cttcattagc tatgggagga
     9901 aatatccttg gaccaatcat cttcttctta ggttggaaca ttattcgttg ggcattcatg
     9961 tggtatacac aagaatttgg ctacaaagct ggttctaaaa ttactgatga cctttctggc
    10021 ggattgttac aagacgtaac aaaaggtgct tcaattttag ggatgttcgt attagctgcc
    10081 cttgtacaaa gatgggtatc aatcaaattc ttaccagttg tatcaactgt aaaattagat
    10141 aaaggtgctt acatcgaatg ggataaattg ccttcaggtg gtgaaggaat caagagtgca
    10201 tttgaacaag ttaataatgg cttagccctt tctcctgaaa aggtaacaac attacaagac
    10261 aacttagacc aattgattcc tggtttagct gcattaggat taacattgtt atgtatgtgg
    10321 ttgcttaaga aaaaggtttc tccaatcgtt atcatccttg gtttattcgt agtcggtgtt
    10381 gttggacacg tcatcggact tttataatcc ataaaaatat agctcgggcg ttgtcccgag
    10441 ctttttttat atagtatact gttcataaac aaatatctgg agggagaaaa tggttcaatc
    10501 aatcaataca aaagtagact tggtaattga tgctacttcg tttacgggtc tgacagatta
    10561 cgggcaaatc atgataggta ataatggttt tgagttttac catgcgcgag atagacgcaa
    10621 atatatccaa attccatggg aagaagtgga ttatgtacta gcatcagtca tgctaaaggg
    10681 aaaatggatt cctcgatatg ccattcaaac gaaaaaaaat ggtacataca cattttcttc
    10741 caaagaacct aaaaaagtgc ttcgtgcgat cagagaatat gtagatgaag atagaatggt
    10801 acaatcattg agtttctttg atgtagtgaa acgtggtatt cgtagtatgt ttaaacgcaa
    10861 gtaaaaaata aagaaaactt ctaagaggag gttttcttta tttttttact ttgcagaaga
    10921 aaatatgaaa tagcaaaacc aattatttgt cccctttaaa tcccatatgc taaaattgat
    10981 tagcaaagaa ggaggttata ttatgagttg gttatggaca ttgattgttg gagccgtgat
    11041 cggagcaatt gcaggtgcga tcaccagtaa aggaaattcg atgggctgtg taacgaatat
    11101 cattgctgga ctagtcggtt caagtattgg acaagcgatt ttcggcgaat ggggaccaaa
    11161 agctgcaggt atggcactta ttccatctat tttaggcgca gtgattttgg tagcagttgt
    11221 ctcatttttc tttggaaaac gatcataagt ttttacaaaa tagtaaacta aaaggagttg
    11281 cagcgagact gtgactcctt tttggcttta tttatttaaa ataaaaagaa gttttccgga
    11341 aatttttatt taactgtcgt gtacgaagaa aagtttgcta taatggaaaa aagcgtgcaa
    11401 agaaagaggg agtttcatta tgatgctacg tgtagaaaaa ttgcgcaaaa aaatgcaaga
    11461 agaaaatttg gattcatttt tggttaccag tccgtacaat cttcgttact taacgaactt
    11521 tacaggtacg acaggattag ctgttattac tttggaaaaa gcatttttta ttactgattt
    11581 tcgttatacc gagcaggcgg cagcgcaggc tcaagggttt gaaattatta aaaatgtcgg
    11641 acctattttt gatgaggtag caaacttagt acgcaaagaa gaactgcact cattagcttt
    11701 tgaggaaaca acagtctcat ttttagaata ttcggtttta gaagaaatca tcgatgcgga
    11761 attagtacct gtttcacaga tgatcgaaga actcagagaa gtgaaagatg aagaagaaat
    11821 cgctattatc gaaaaagcat gtcatattgc ggatatggca tatgatcaca tcctgaaaat
    11881 gatccaaccg ggcatgactg agattgaagt cgcaaatcaa ctagactttt atatgcgttc
    11941 tcttggcgct tcaggtgttt cttttgatac gatcgttgct agcggattgc gttcggctat
    12001 gccgcatggt gtagcaagca aaaaaatcat cgaacaaggt gacttgatca cattggactt
    12061 tggttgctat tacgaagggt acgtttccga tatgacacgt acattcgcta ttggtgatcc
    12121 tggggaaaaa ttaaaagaaa tttatcaaat agtcttagat gctcaattag cagttattga
    12181 tgctgcaaaa ccaggaatga ctggtgtcca attagatgca gttgctagag attatatttc
    12241 aaaacatggt tacggggaag catttggaca ttctacagga catgggatcg gtttagagat
    12301 ccacgaagga ccaaacgttt cttctcgtgc agaaaagcaa tttgtaccag gaaatgtgat
    12361 cactgatgaa ccagggatct acttaccagg aatcggtgga gtacgtattg aggatgactt
    12421 gttgatcaca gaagatggaa atcgtgtgtt gactcgttca ccaaaagaat tgatcatctt
    12481 atagtttcat caatagaaga agtaagcgta aaaatcagtg ttcacagact ttgttataga
    12541 aaatcactcg atttaatgat acactataga agaactttta agctgtaaga gataataaag
    12601 ttgggtgttt tctccaattc atagcagttt gcagctgatc ggcggcagtt ttcttaccac
    12661 gtgatcacta tgccgcatca tagagaattc ggatgtatct cgtgcttagg caaagggagg
    12721 aataaataaa tggcagatga aaaaaatttg attttaaacg caaaccaaga tttaggagag
    12781 atcgtgatcg ctcctgaagt catcgaagta attatcggga ttgcagcttc taaagtagaa
    12841 ggcgtatacg gaatgcgcgg aacttttgcc agcaatgtca ctgaattatt aggacgtgca
    12901 gctcacggaa aaggtgttta cttagcaaac gaggaagatg gcttgaaagt agatctttac
    12961 tgttatctag aatatggtgt ttcagttcct aaagtagcaa tggaaatgca ggaacgggtc
    13021 agccagcaag tccttttcat gacggatatc gatttatctg aagtcaatat ccatgtagta
    13081 gctgttgttc ctgaaaaatt agaacaacca acaatcgaag aactttttga agacgaagag
    13141 gaagagaatg aataaagaat tatcaagaca cgagattcgg gaaatggcat tgcaggcttt
    13201 atttccatta gacttcaatg cagacctaac aaaagaagat gcgattttca acgcaatcga
    13261 attagaccac cgcgacatga tcaacgaaga tgaatcagag tttgttcctg tttatcttga
    13321 tacgctagtt ggcggtgtct gtgcaaaaaa agatgaattg gataaagtga ttgaaaaaca
    13381 tttaaaaaac aattggcaca tcaatcggat ctcaaaaatg gatttggtga tcttgcgtat
    13441 cgcgattttt gaaatgactt atgtgacaga tgtaccagcg gctgttgctt taaatgaagc
    13501 aatcgagttg gcaaaaacat ttagtgatga ccgttcacgt aaattcgtca acggtgtttt
    13561 atccaatgta ttgaaagaat tagaagctgg ggcgtaagtc ctagcttttt tctatgaatc
    13621 atcataaacc tgttataaat cgggaaaatt ctatggtaga atgaaaacac aaaaaatatc
    13681 tgggagtggt tagatggcag aattgattaa cggaaaagaa ttagctgaaa aaatgcaggc
    13741 agagattgct gaaaaaatcc aaaaattgaa agataatggt atccatcccg gattagtcgt
    13801 tttattggta ggggaaaatc cagccagcca aatctatgta agaaataaag aacgtgtagc
    13861 aaaagaaatc ggaatccatt cattggtaga gcgttatcct gagacgattt ctgaagacga
    13921 actacttgca gaaattgaaa aatacaatca agatcctagt tttcatggta tattggtcca
    13981 attaccattg ccaaagcata tagatgaaga aaagatcctg ttagctatcc gccctgaaaa
    14041 agacgtagac ggttttcatc cgatgaattt aggcagactc cttgcgggta aaccggacaa
    14101 gattccttgt actccatacg gcatcatgaa aatgtttgaa gcttattcta tcgatccggc
    14161 gggtaaacga gcagtagtta tcggacgaag caatatcgtc ggtaaaccga tggctcagct
    14221 actcttgatg gcagatgcaa ctgttactgt tgctcattct aaaaccgaaa atctggctga
    14281 actggcacaa gaagcagata ttttagtcgt agcaatagga cgtggacatt ttgtcacaaa
    14341 agaattcgta aaaccaggtg ctgtagtgat agatgtaggg atgaatcgtg atgctaacgg
    14401 taagctgatc ggtgatgtga aatttgatga agtggagcct attgcaggct ggatcactcc
    14461 tgtgccaaag ggtgtagggc caatgactat cacgatgctg atgtatcaaa ccgtaaaatc
    14521 tgccgaagct tttggagcag gtgaatagat ggcacaagaa tatttaactg tcactacatt
    14581 aacgaagtat ttgaaaagaa aatttgatgc cgatccttat ttagaacgcg tatatttaac
    14641 aggcgaaata tcgaatttcc gtttacgtgc caatgcccat caatatttca gcttaaaaga
    14701 tgaccatgca aaaatatctg ctattatgtt caaaggagca ttccaaaaat tacgattcca
    14761 gccaaaagaa ggaatgaaag tccttgtcgt tgggcgtatt tcattatatg aagcgggagg
    14821 ttcttatcaa atctatgtag agcacatgga gcctgatgga ataggtgctt tatatcaaga
    14881 attggaagaa cgaaaagaaa agctgatgaa ggaaggcctt tttcaagggc ctaaaaaaat
    14941 gctgccacgt ttcccaaagc gaatcgcggt ccttaccagt ccaagcggag cggttatccg
    15001 cgatatcatc acgacggtga aaagaagata tccaattgtc caattggttt tgttcccaac
    15061 agttgtacaa ggaaaccaag cagcggacaa tgttgtcagg aatatccaga gagtagaaga
    15121 gatgggcaat ttcgatacaa tgatcatcgg tcgtgggggt ggttcgattg aggatttatg
    15181 gccgtttaat gaagagcggg ttgctcgggc gatttatcaa gctaatacgc cggtgatttc
    15241 atctgtagga catgaaacag ataccacgat cgctgattta gtagcagatg ttcgggctgc
    15301 cactccaaca gcagctgcag aattagctgt tcctgttttg tcagaagagt tgatgcgcat
    15361 tcaagaacgg caagctagat tgcagcaggc gtttgtacgc cagatccagc gcaaacaaga
    15421 acggttcgag cgtgctcgac gttcctatgt atttcggcag cctgaaagat tgtatgaagg
    15481 tcagaccatc aaattagacc agttgaaaca gcgattatat caagctacac agcaaattta
    15541 tcatgcgaaa gaaaaacaaa cgatcgcatt aagccaccaa ttggaacaag tggctccgct
    15601 ttatcgagtt aaagcagctc gccaagaaac tgcttatcta gaaaaacgct tagaagagaa
    15661 aatccaacaa tatatgaaac aacagcagca gcgttttgct caagctgttc aatctcttga
    15721 tttgttgagt ccattgaaaa tcatgggaag aggctacagc tatacgacgt taaatgatca
    15781 agtagtaaaa agtgtcaccg agattcatca aggggatttg ctgacgatcc attatcaaga
    15841 tggaatcgtt aaaggacaag tacaagagat agacaaagga gaataaaaat atggaaaatc
    15901 caacttttga agagtcactg caagaattag aaaaaattgt catgcagtta gaacaaggag
    15961 acgttccatt agaatcagct ctagattcgt ttaaacgagg aatggaatta agcaaacatt
    16021 gccaagatac attagctaaa gcagaagaga ccttgacgaa aatgatgaca gaatcaaatg
    16081 aagaagttga ttttgacgga aacgaggaaa gctgagatga gattaactga cttttcagct
    16141 attcatctac ctgctgtaga aaaagaaatc ttgtcttttt tagatgagca tacaacggat
    16201 agaggactct atgatgcgat gagttattca gtgaaagcag gcgggaaaag aatacgtccc
    16261 ttattacttt taacggccgt tgcttccttt gatgagccta ttgatgttcc cgtctatcaa
    16321 gtagcagctg ctttagaaat ggtgcatacg tattccttga tccacgatga tttgcctgcg
    16381 atggacaatg atgatcttcg tagaggaaaa cctaccaatc ataaagtatt cggcgaagca
    16441 ttagctatat tggccggaga tggtttgctt acaggagcat tccagctaat cagtatggca
    16501 cacttaggta attctcctaa gcttctgctg cttcagcaat tagctgtttg tgcgggatca
    16561 caaggtatgg ttgctgggca agctgcagat atcgagggcg aatcgaaaaa gctttcttta
    16621 gaagaattag catttattca tgaaagaaag acagggcatt tgattcgtta tgccttgctg
    16681 gctggtggca tattagcgaa gcaacctgaa gaaatcttat tgctacttca acgattagcc
    16741 gaacatttag gtttagcttt tcagataaga gatgatctac tcgatgtgat tggtacaacg
    16801 aaaacattag ggaaaacagc tggtaaagat gaacgtatgg aaaaaaatac ttacccgcgt
    16861 ttattaggcc tagaaaagac acgtgaggcg ttggagattg agctgttatc cgccaacaag
    16921 atcatagaca aattagaaga ggacgttttt tcttttgatg gcgggctgat gcgccagatg
    16981 atcaagcaat ttgatgtgga gaaatgaggg aaagaatgga aaaagaacgc gtagatgtat
    17041 tagcggtcaa acaaggatta tttgaaacaa gagaacaagc caaacgttca gtgatggctg
    17101 gtttgattta caacgaaaaa aacgagcggt ttgataaacc gggagaaaaa attccagtga
    17161 gtagtgaatt aagagtcaaa ggtaaaaaac ttccttatgt ttctcgaggg ggattaaaat
    17221 tagaaaaagc actcaaagca tttgatctat ctgttgaagg aaaaacattg ttagatattg
    17281 gtgcatccac tggcggattt actgatgcag ctttgcaaaa tggcgcaaaa atgagttatg
    17341 ctttagacgt tggctacaat cagctagctt ggaaaataag acaagatcct agagtcgtcg
    17401 taatggaaag agtgaatttt cgttatgcaa aacccgaaga ctttacctta ggaattccag
    17461 aagttgctgt tatcgatgtt tcttttattt cactaaaact gatgctgcct ccattacatg
    17521 ctattttaaa agaagaggga gaagtgattg ctttaatcaa accccaattc gaagcaggca
    17581 gagaagctgt agggaaaaac ggaattgttc gtgaccctca gacccatcaa aaagttttag
    17641 aagacatttt gtcatttgcg gcacaaggtg gatatgatgt gctggaatta agttattctc
    17701 cgatcacggg gggagaagga aatatcgagt tcttggctca cttgaggaaa gtacccgaaa
    17761 gcggaacaat caattcagct attaatatgg cagaagtggt ttcaaatgca cacgaacagt
    17821 ttgaccataa ataaattgta actgtgaaaa tcaagtgaga agtatgcgga ttcttcttta
    17881 ttataccaag gaaataagct atcattgatt aagaagaata tgctaaggag cgaagacgta
    17941 atgagaaaaa aagatcggca tcgtttgatt actcgattgt tgagtgagca agatatccga
    18001 aagcaggaag aatttgttga aatcctgaaa agcaaaggaa tatctgttac tcaagctacg
    18061 atttccagag atataaagga actgaaactg atcaaggtac cggcttcaga tggcggctac
    18121 cgttatagca taccagctga aacaaacgaa gatgtaaata ctaaattgga aaaactatta
    18181 aaagacgctt tcgtggcagt tgaccaaatg gaaaaatttg ttattttgaa aacattgccg
    18241 ggaaatgctt cggcagcagc aaatttgatt gataaacgtt acaagaaaga attattttcg
    18301 attatcaatg atgacgataa tgttttgatg attacacgaa ctgaagacca ggcagtttta
    18361 ttgaagaaag acttcctgca ttacctttaa gttgaggagc gaatgtgatg ctacaagaaa
    18421 tcagtattac taactttgcg attattccgg aattgcggct ttcttttcac gaaggaatga
    18481 ccgcgctgac cggagagact ggtgcgggta aatccattat tatcgatgct ttaggtctgt
    18541 tagcaggagg aagaggttca agcgattata tacgtcaagg agcagaaaaa tgtgtgcttg
    18601 aagggttgtt tgaactacca aaacaagaag gattctctga actgatggtt gaattaggga
    18661 tcgaaacaga tgaggacaat ctgattgttc gacgggatat gtccttaact ggaaaaaatg
    18721 tttgtcgagt gaatggtcat attatcacat tagctaatct gagaagaatc ggcagttatt
    18781 tagtagatat ccaaggacaa aacgaacatc aggaactgct ccagcctgaa tcacatcttg
    18841 ctttattgga tcgttttgga gatgtagctt tccaacagaa gaaaaagagt tatcagcaag
    18901 aatatatctc ttatcgtgag ctggaaaaaa gggtacgaaa agtgcagcaa aatgaaaaat
    18961 cctatgttca gagaatagat atgctccatt tccagcaaga agaaatagct gctgctgatt
    19021 tgcaagtcgg agaagaagag aaattaaaag aagaaagaga aaaacttagc aattatcaaa
    19081 aaattgctga tggattagct gcaggttacg gagcactgac gaataatgaa caaaacagtg
    19141 tagatggtgt tggcttggcc gtttcagaac tgcaagggat cgcccatcta gatgtggagt
    19201 atgaagcaat ttatgaaaat atccaaagtg cttactattt gctgcaagat gcaatcggcg
    19261 atatgagtcg tcagattgat ttattggagt tagatgagag tcgtttagaa gaagttacgc
    19321 aacgtttaga attaatccga cagttgaaac gtaaatacgg cgaatccatt gagtcaatat
    19381 tggcttacta tgacgaaatc accgaagagc tagcttcttc tgatttttca gaaggtcaat
    19441 tggacaaaat gaaagaggaa ctagagcaaa aagagcttct acttcagcaa caagccgcag
    19501 atcttcatga agcgcgtaaa aaaatcgcaa aagaattgga aaaatccatt ctgcatgaat
    19561 tgaaaagtct gtatatggaa aacactgagt ttgaggtacg tttcttaaaa gaagaaaata
    19621 gacaattgaa tcatgatgga tttgaccaaa tcgaatttta cattacgacc aatccaggag
    19681 agccactgaa gcctttggtg aaagtcgctt ctggtggcga actgtctcgg atgttgcttg
    19741 ctttgaaaac tattttttct tctgagcaag gtgtgacaag tattattttt gatgaagtgg
    19801 atacaggtgt gagcggaaga gttgcacagg ccatcgcaga taaaatttct caaatatcga
    19861 aatactctca agttttgtgc atcactcatc tgcctcaagt tgctgcagtc gccgactatc
    19921 aatattatat tgtgaaagca gtgattgatg gacgtacgca aacttctgtt tcagagttga
    19981 aaacaaaaga aagagaagaa gaaattgcac gtatgctggc tggaagtgag attacaaaac
    20041 tcacactcga acatgcaaaa gaactattga aactcgcaaa aaggacctga gccacctcgc
    20101 agtggttcag gtccttttca gcagctgata gctatatttt atttttacat gacactcatt
    20161 acagcagtca atacaacaga tccaatcgtt aaaatcagca ttgtccaaat gacaaatttt
    20221 gttactttag caaaagtact tgtttgtttt ttttcacgca ttgttttcaa cctcgtcatt
    20281 aattagttga ttcaagtgta caatcttttt tccatttcca caagaggggt tttttataaa
    20341 aacttccctt tttttaaata ataggtagtt acgatacaaa taaataatgt taatagaact
    20401 agccatataa aggcatcatc cctttcagca ataggtaact taatgttcat tccatagata
    20461 ctgccgacga ttgtcggtat agttaaaata atcgttaaag aagttaaaat tttcataata
    20521 ttattcagat tattcgatac gattgctgaa aaagtctgat tcatttgttc tactagtttt
    20581 aattgaatgc gagaagtagt cattgcctgc tctgtttcca ccaatacatc acgtaaatga
    20641 tttttcaaag catgattttc ttggaaaagt ttattttgcg ctagttcttt caatgcatct
    20701 aaattagcag aagtcgcttc tctgaaataa acgaggcttt tttggatatc catgatttga
    20761 taaagttgtt tgttttcagt ggatacttga agtttggctt ccaaagtatc gcattgagat
    20821 tgaagcgtag ctaaataact gttgtaagag agtgtgacct gccaaatgaa ttgaaagaag
    20881 atattgagat taggcgaaat atcacttttt ggaaaggtct ggctaaagac tttctttaaa
    20941 aacagtggtt catgattcgt aattgtgata agcttctttt tgggcgtcaa aataatagag
    21001 agcgggtatg tatccacttg aaaataaccg ctaggacttg ttttagcaaa aggatattgt
    21061 aacaataata acgctggctt tgtcaacttt gattgattga attcttctgt acgggaattt
    21121 tctgcatcgt ccaatatgga agtgatgtaa tctgacggaa tttcaaattg atgtgtcagc
    21181 tgatgaatct cttcttgatt aggacgttct acacaatacc agatcgtttg gtcagtttcc
    21241 tttccggtat tacgcagttt ctcattatct aatgtaaaat atttgatcat gattttcctc
    21301 ctgttgcctg tttcttttat tataacggat agggaattgg aatacatgga atgtgtttgt
    21361 aagatataga atataactgc ataaatttaa ataaaatgaa acagttgtgt aaagtatata
    21421 cagtatactt gaagcaacga taaaaaagga gtttacgttc atgggcacat tcacaccact
    21481 tatcttgttt tggtatattt acccggtaat cgttttgttt ggttgccaat ttcttgtaaa
    21541 attattttcg ttaaatcgtc gttttaaaat aaaagcccct gatttggcga ttcctttttt
    21601 gtggggcggt atgcatgcat tgtctagaaa tacagggaca atttctatct taccgttttt
    21661 ctttatttct gttttgctaa tgggaatttg tattgctgtt ttccaagcat attattatga
    21721 agaaattatt tatccgcgtt atttcaagat gacttggcgt ttggtttttc tgttgacaat
    21781 cgtcttgtac gctgtcttga tcattgtgaa catactttct tacctatgaa aaaaatagaa
    21841 aagctgtaaa aagtctttta aaaggctttt tgcagcttga tttttaaaaa aatgtttatg
    21901 aataaaaaaa gttgttcccg gatttcccag aaaaggtggt agaaagtggg ggattgtggt
    21961 agactaattt tagttagtgg aggaatgggg ggctttcaat gttgatgggt gaattccaac
    22021 ataatattga tgcaaaagga cgtttaatcg tgccctcaaa acttcgtgaa gaacttggcg
    22081 aaaagtttgt tctgacacgc ggattggatg gctgtctttt cggctatccg atgagcgaat
    22141 gggaaaatct ggaagctaaa ctgaacgaaa tgccgttggc taaaaaagac gcacgtactt
    22201 ttgtgcgatt cttctattcg gctgccactg aatgcgaact tgataaacaa ggacgaatca
    22261 atatacccag cacgttaaga aattatgcag ctttgacaaa aggttgcgta gtcatcggtg
    22321 tatctaaccg aattgaaatt tgggatgaga cacgatggca agatttttct gcagcagcag
    22381 aagagaactt tgacgaaatt gctgaaagta tgattgattt cggactataa gaataggaag
    22441 aggctgacta atgactgaaa catttcaaca ctatacagtc atgttgaaag aaacggtaga
    22501 tggattgaac atcaaaccgg atggcgttta tgtagactgt acactcggcg gtgccggaca
    22561 tagtgaatat ttattaagcc aactaagttc taaagggcat ctgtatgcgt ttgaccaaga
    22621 tcaaaaagca atcgatcacg ccaagctgcg tttagcttct tatgtcgaac aaggtatggt
    22681 cacatttatc aaagcgaact tcagagaatt agaagaacgc ttgagtgagc atgtagataa
    22741 ggtagacggc attttgtatg atctaggggt ttcctctcct cagctagatg aagcagaacg
    22801 tggatttagt tatcatcaag atgcaccgtt ggatatgcgc atggatcagt cagctccttt
    22861 atccgcttat cacgtaatta atgaatattc ctaccatgaa ttagtaaaga ttttcttccg
    22921 ctatggcgag gagaaatttt ccaaacaaat tgctcgtgaa atcgaacgag tacgaaaaac
    22981 aaaaccaatc caaacaactg gtgagcttgt cgaaatcatt aaacaagtca tcccagctcc
    23041 cgcaagaaga aaaggcgggc atcctgcaaa aagaattttt caagctgtgc ggattgcagt
    23101 aaatgacgaa ctcggtgccg aagaggcctc tttagaacag gcaatccggt tattgaaagt
    23161 caatggaaga atcagcgtaa tcacattcca ttcattggaa gacagaattg tcaaaagtat
    23221 gtttaaagaa tatagtacgg tacaagatct accaccggga cttcctgtca tacctgacga
    23281 attccaacca gaactgaaag tcattactcg taaaccaatc cttccttcag aggaagagct
    23341 aacagaaaac aatcgatcac gcagtgcaaa gttacgtatt gcagaaaaaa taagaatgaa
    23401 agaggagtag aatgatggca gaattaaaga aaatgcaaga ttacgaatat gatttacctg
    23461 aattggtgga tcagccggaa caacaaatgc ctgattcttc ttcgaatcaa gaaatcattc
    23521 ttccacaatc accggcaaaa cgattaaagc agatttctgc tttggaaaaa attgctgtgg
    23581 caagcgtggc gattgctatt gttgctttat gtgttttaac agttatgctg cgcacaaata
    23641 tcagcggagc tgaaaaagaa atcacgtcaa tacaaacaga aatcaatgat aaaaagcaag
    23701 aaaaaacgag tttagaacaa gaaaaaaacg aactttctcg tactgaacgg attaaacaaa
    23761 tcgcagaaga aaaaggattg tctattaatg acgacaattt aaggaaagtg aagtaaatga
    23821 gtcttaaaaa taaaattcgg cggtttatgg aaaagaaaaa tctaaatccg atgaacaatc
    23881 gcaaaaaagt aggtatcatt ttatttgcta cgagtattgg attgttcttt ttatttgccg
    23941 tccgtttctc gtatatcgtt attggaggtc atgttgctgg tacctcatta gctgaaaaaa
    24001 caaaacagtt gtaccaagga agtgaagtag taaaagcaaa aagaggaacg atttatgatc
    24061 gtaacggtgt ggcgctcgca gaagatgcct cctcttattc aattaaagcg atactctcaa
    24121 aaacatatac ctctggggat aaaaaactgt atgttgaaga aaagaatttt gataaaatag
    24181 cagaaatcct gcataagaat ctttctatag acaaaaaaga tgcactgaat attttagagg
    24241 acggcgcaaa aaaagagctg tatcaagttg aatttggtag ctatgggaaa aatatctccc
    24301 aagaaaccaa gcaaaacatc gaagcggaca tgaaaaaaga aggtgtcgct ggtctttatt
    24361 ttgtcgacca tcaggctcgg atgtatccaa atggcgtctt ttcttctcat tttattggat
    24421 acgcagttcc agacaaagat gagaatggct tagttggtaa actaggtttg gagtctgctt
    24481 acaatgatat cttgagcggt aaagatggta agattattta tcaaaaagat aattttcaaa
    24541 atcctctgcc aggtacagtg gcagaagaag aaaaagctgt cgacggacaa gatatctaca
    24601 cgactttaga cagcagacta caaagttatt tagaaacact gatggatcaa gtaaatgaag
    24661 aatatcagcc agaagaattg acagcagtcc tgatgaaagc aaaaaccgga gaaatcttgg
    24721 caatgggaca acgacctact ttcaatcctg aaacaatgga aggattgact ggaaaagatg
    24781 ctatctggcg taatttcttg gtacaagata gttatgaacc aggatcaacc atgaaagttt
    24841 tcactactgc agctgctatc gaagaaggag aattcaacga gaatgaaacc ttccaatctg
    24901 gaaaaatcca agtagaagat gctacgatca atgaccatga ctttggtgaa aaaggtgtgc
    24961 tgaccatgcg tcaagctctt tcatggtcaa gtaacgtcgg gatggtcata ctggaacagc
    25021 gattaggcgg acgatggtat aactatttac aaaaactggg atttggacaa agtactcatt
    25081 ctggattaga tgatgaagta aacggtgctt tacctacatt aaatattgtc gaccgagcaa
    25141 tgagtgctta tggacaagcg gtaggtgtga cgaatttcca aatgatgaaa gctttcactt
    25201 ctattgcgaa taacggtaca atgattcaac ctcgatatat cagcaaagtg gtggatcccc
    25261 aaactggaga agagcgaaca actcaaacag aagttctagg gcaacctttt tcaaaagaaa
    25321 caacagaaaa agtccgtgaa tatatgagag atgttgtcga aagcgaaaac tacggtagtg
    25381 cttatggtgt atatagtgta cctggataca atgtatcggc aaaaaacgga acagcccaaa
    25441 tcgcttctga tacaggggga tatcaaacag gagatacagc ctacctgtat tcgattgtgg
    25501 agatggttcc ttctgaagat ccagactatg tcttgtatct aacaatgaaa caccctaaga
    25561 catatgatcg aatggcattg gctaaaatcg caaatccatt gatgaaacgg gcaatggatt
    25621 ttaaagaaac tgaagaagat tcagatacag aaacaaaaac agaaaaagta tccgtagccg
    25681 attatcgtaa tcttgaagcg gacgttgcag cagctgatgc tcaaaaaagt ggactgcaac
    25741 ctgtagtgat cggtaatggt aaaaaagtac aaaaacaatc tactgccaac ggtgatcagt
    25801 tgatctctgg agaaaaactg atcctatata ctggtggtga taagctgatg cctgacgtaa
    25861 caggatggtc aaaagcagat atcatgaagc tggggaaaat cctcggtatt gaagttagtt
    25921 ttgatggaga tggttattgt gtcaaacaag aacttgctcc atatgaaaaa atcactgaag
    25981 acaaacttag ttttacttta gaagaataag aagagaaata ggagagaaaa gagcatggaa
    26041 tggacacaag cattgattcc cattgtcagc agttgtgcaa tgacaattgc cgcaatgccg
    26101 ctatttattg gttattttca aatgaaaaag caaggtcagg cgattcgtga ggagggacca
    26161 aagtggcaca atagcaaagc cggaactcct acaatgggcg gcttagtgtt tctgatcggc
    26221 tctattttga caggaatttg ggttggagct tggcaaaaac agctaactcc tactcttttc
    26281 atcttgcttt ttgtattagc gttatacgga gtaatcggct ttcttgatga ttttataaaa
    26341 atattcaaga aaagaaacat gggactgaac tcgaagcaga aattgattgg acagattcta
    26401 gggggaatca tcttttatct tgtttaccgt tctgaaggtt atccaggtgt attgaatttc
    26461 ttcggactag atctaccttt aggaatcatt tatggtattt tcgctctctt ctggcttgtt
    26521 ggattttcaa atgcagtaaa tctgacagat ggcatcgatg gattagttgc tggcttagga
    26581 agtatctctt ttgcagctta cggtctaatt gcttggcatc agcatcagta tgatgtatta
    26641 atcatttgct taagtgtttt aggaggttta ctgggtttct tcgtctacaa cagaaaacct
    26701 gccaagattt ttatgggaga tgtcggttca ttagcgttgg gcggactttt agcggcaatt
    26761 tctataatgc taaaccaaga atggacattg cttttgattg gtttgatcta tgttatggaa
    26821 acagcaagtg tgatgcttca agttacttcc tttaagctta caggaaaacg aatttttaaa
    26881 atgtctccaa ttcatcatca ctttgaaatg tgcggatggt cggagtggaa gattgataca
    26941 gttttttggt tgatcagcat tgtgacttcg ttgatcacat tatggatcgt ttggtaggag
    27001 atgacgggaa atgaaaaaaa tatcaacata tgaaaataag aaagtgcttg tcttaggact
    27061 cgcaaaaagc ggagtaagtg cggccaaatt attacatgaa ttgggcgcac ttgtcaccgt
    27121 taatgatgga aaaccttttg acgaaaatcc ggaagcacaa gagctgcttt ctttagggat
    27181 aaaagtaata acaggtagtc atccaatcga attgttggat gaagaatttt cattgatggt
    27241 caaaaatcca gggattccct acagtcatcc tttagtagca aaagcccaag aaatgggtat
    27301 tccggtaatt acggaagttg agctagctta cgaagtagcg gaatgcccaa ttattgggat
    27361 tacaggaacg aacggaaaaa caacgacaac aacgatgaca ggactactac tgaatgcagg
    27421 agcagatcaa ggaattgcac gtttagcggg taatattggg tatccagcaa gtggtgtagc
    27481 gcaagaagca aaatcagaag ataaaattgt gatggaattg tctagcttcc aactcatggg
    27541 aatcacagat ttccgccctc atatagcggt gattaccaat atctatgaag cacatattga
    27601 ttatcatggt acaagaaaag aatatgtcaa agctaaatgg aaccttcaaa aaaacatgac
    27661 agaaaaagat tatctgatcc ttaactggaa tcagtcagaa ctacaagaat tagcgcaaag
    27721 aacaaaagca cgcgtactgc ctttctcgac aaaagaagtg ttggaagacg gtgtgtatgc
    27781 tgacgattac tcaatatact ataaaaaaga aaaaataatg gaaatatctg aacttggcgt
    27841 accaggtaag cataatgtag aaaatgcact agctgctatt tctgtagcaa aattgtatgg
    27901 tatatcaaat gaagctatac gagaaacgtt gcattttttc catggcgtac cacatcgtac
    27961 gcaatatgta ggagaaatcc aaggcagaaa attttataat gattcaaaag caaccaatat
    28021 tttagcgact aaaatggctc taagtggatt tgaaacgtct aaggtcgtgt tattggctgg
    28081 cggattagac agaggaaata catttgatga attaattcct tcattaaaag gaatcaaagc
    28141 aatggtcgta tttggccaaa caaaagaaaa gttgatggat gctggaaaaa aagcaggcat
    28201 cgagacaata gtaacagccg attctgtaga acaagcagtt cctttagcat tagaaaacag
    28261 tacagatggt gatgtagttt tattatcacc agcgaatgcc agctgggatc aatacccaaa
    28321 ttttgaaact agaggaaaca gattcatgga agctgtgaac cgattaaaat agaaaaagta
    28381 gaagcgagcg ataaaaaatg aaaattttgg ttacaggcgg aggaacaggg ggacatattt
    28441 atccagctct tgcctttgtc aattatgtaa agactcaaga accgaatgct gaattcatgt
    28501 atgtgggagc aaaacgaggt ctagaaaaca aaatcgtccc tgatacagga atgcctttcc
    28561 ataccttgga gattcaagga tttaaacgaa aaatttccat gcataacatc aagacggtcc
    28621 gattgtttct taagagtatc cgtcaagcaa aaaaaatcct cagtgatttt caaccagata
    28681 tcgttatcgg tactggagga tatgtttccg gagcggtagt gtacgctgca gccaaaatgg
    28741 gtattccaac agttatccat gaacaaaaca gtgtaccagg tgttaccaat aaatttttaa
    28801 gccgatatgt ggatcggatc gctctttcat ttaaagatgc ggcttccttt tttcctgaaa
    28861 acaaagcagt tcttgtaggt aatcctcgag cacaagaagt agcagatact aaaaaatcag
    28921 atattttgaa aacatttgat ttggatccag aaaagaaaac agtcttgatt tttggcggta
    28981 gtcaaggagc acttaaaatc aatcaagcag tatcagtttt tttaagtgaa tatattcctt
    29041 cttcttacca agttttgtat gcttcaggag atagatatta tgaagagatc ctaaataaaa
    29101 taaatcctga aatacaaaat attagtgtcc agccttatat caaaaatatg gcagaggtca
    29161 tggcttccag tgatctgctg gtggggcgtg ctggtgcaac ttcgatcgca gaattcacag
    29221 ctctggggtt accggcagtg ttggttccca gcccatatgt tacgaatgat catcagacta
    29281 aaaatgcaat gagtcttgtt cacgcaggag cagcaaaaat gattgcagat aatgaactaa
    29341 caggtgaatc actaagtcag acagtcaacg aaatcatggg tgatgaagaa ttgcaaaagc
    29401 aaatgtgccg agcttctaaa gaacaaggaa tccctgatgc ttcaaaacgt ctttatgact
    29461 tagtaaaaca aataatcaaa aaataaaggc ggtggtctta gtgagtaaga aaaaagaacc
    29521 aaaaaaattg tctcatccgt cttctgatga aaacaatttg actccttggc aaaaggctaa
    29581 ccgaaaatat ctggcagaac aagagatgaa aaaccaagag ccaaaaaaag aagacataac
    29641 attagataca cccctttcag attctgaaga agaaaaaacg gttcaagagg aacagcaaga
    29701 agaaagccag tccgagaaaa aaatcgaatt ttttgaagag atcaagcaaa agaaaaacgg
    29761 tggaccatat aacggttctt ttttgaaccg gctgcccaat ttaaaaagtc aacggcacaa
    29821 agtactattt cgtcggttag ctttgatcat cactgtattg ggtataccgc tgattttttt
    29881 gatctattat gtttctcctt atagcaaatt acaagcagtt gcagtatcag gcaataagac
    29941 agtcaacagc caagaaatca tctctgatac aaaattgtca ttaggagaaa atgtttgggg
    30001 acagtatttt catcgatcta cttatatcga tcgccttaaa aaagcacagc caagaatcga
    30061 aacagcaaat atacatttca aagggatgaa tgaattcgat ttggatgtaa cagaatataa
    30121 agagattgct ttgatcgcaa aaaacaatca atactaccca gtgattgaaa atggaacagt
    30181 attagatgaa aaagtagcaa atcctacaaa aaatctccct atcttggaag agttcaaaga
    30241 ctctgcaaaa atcaaagaat taacaaagca atacaatcag ttatcatcag aattacaaaa
    30301 agcaatttct gaaatcaagt acacgccaag agcaagcaat aaaaatctga tccaattgaa
    30361 tatgaatgac ggcaatcaag ttattgtaaa tatcaataat cttgcgaacc aaatgaaata
    30421 ttattcgcaa gtagccaaag atatggatga aaaaggtgtg atcgacatgg aagttggtat
    30481 cttctcgcat ccttacggta gcactcaaga aacggaagaa tcgggccaat cggaagaaac
    30541 aactgaaagt gaagaaaatg cggatcaaac aaacgaatca agtgaagagg cagaaacgca
    30601 aaaagcacaa acacctggat cgtctagcac aaacggatca tctgattcaa atgattcaag
    30661 tgaaacaagt caaatagaag ataatccaag tacaactccg tcaagtttaa atgaaaattg
    30721 ataaaacaac tttaaaaagt aagaatgtac tttctaaaaa ccagtaattt atggtagaat
    30781 tggaagcagt gaatatatct attaataagt atattttaaa acggacgcta gttgttcgct
    30841 tgtatatgga atagtaagta ggagggaatc ccattcatgg caaaaacagg aatgtatgta
    30901 ggccttgata ttggaacgac atctgttaaa gttgtcgtgg ctgaatatat cgacagccaa
    30961 atgaatatta ttggagtagg aaacgcaaaa tcagaaggga ttaaccgagg cattatcgtt
    31021 gatatcgata aaacggtcca agcgatacaa cgagcagtac gacaagcaga agaaaaagca
    31081 ggtatccaga tcaaaggcgt aagtgtcggt ttgccagcaa acatgcttga agtagaaaat
    31141 tgccagggaa tgattgcagt taacggagat tcaaaggaaa tcaccgatga agatgtcaga
    31201 aatgtggctt cagctgccct tgttcgttca atacctcctg aacgtcaaat cgtgtcaata
    31261 ttgccacaag attttactgt cgatgggttc gagggaatca aagatcctcg tggaatgatc
    31321 ggcgtaagat tagaaatgta cggtctgttg tttacaggac ctaaaacgat catccataac
    31381 atacgtaaat gcgtggaaaa tgccggatta atcgtcaacg aaatggtgat tactccttta
    31441 gcgctgactg aatcaattct ttctgacgga gaaaaagatt ttggtacgat cgtcattgat
    31501 atgggtggcg gccaaacaac aacggctgtt atgcatgata agcaattgaa gtttaccaat
    31561 cttgaccaag aaggtggcga atttgtcact aaagatatct ctattgtatt aaatacgtca
    31621 tttaacaatg cagaagcgtt gaaaataaac tacggggatg catacccaga acggacatca
    31681 ccagatgaag aatttccagt agatgtgatt ggtcagtcag aaccagtgaa agtcgatgaa
    31741 cgctacttgt cagaaattat ttcagctcga atggaacaga ttttcaacaa agcaaaagat
    31801 gcattggacc aaatcgaagc attagagttg ccaggtggcg tcgtattgac aggcggagct
    31861 gctagtcttc ctggtgttgt ggacttggca caagaaatct tcggcgtaaa tgtaaaattg
    31921 tatgttccaa atcacatggg gctgagaaac ccggtattta caaatgtaat aagtatcgtg
    31981 gattattcag caaacttgag tgaagtgtat caattggcaa aaattgctgt tacgggtgaa
    32041 acctcagcag ctcgtcaaat ggtagttgaa caagaaacaa caaacacata tgagagctat
    32101 gaagcacctg aagaaacaat ctacgatgag ccggaaccaa aagagtccgg agaaaatgta
    32161 aaaaataaaa tcaagggttt ctttacaaat atttttgatt aattcatcct gggaggaata
    32221 agatatggaa ttctcaattg ataataatat caatgacggc gcagtaatca aagtcatcgg
    32281 agtcggtggt ggcggtggaa atgctgtaaa ccgaatgatt gaagaaaacg ttaaaggtgt
    32341 tgaattcatt acagccaaca cagacgtgca agcattaaaa aattcaaaag ctgaaacagt
    32401 tatccaatta ggccccaaat atactcgtgg tttaggtgct ggttctcagc cagaagttgg
    32461 acaaaaagcg gcagaagaaa gtgaacaatc cttacgcgaa gctttagacg gtgcagatat
    32521 gattttcatc actgcaggaa tgggtggagg aaccggtaca ggtgctgcac caatcgttgc
    32581 aggtatcgcg aaagaattgg gcgcattgac tgttggtgtc gtgacacgtc cattcacttt
    32641 tgaaggaccg aaacgtggcc gttttgctgc agaaggtatc gctcgtttga aagaaaatgt
    32701 ggatacatta ttaattatct caaataaccg tttattagaa gttgtagata aaaagacacc
    32761 gatgcttgaa gctttccgtg aagcagataa tgttttacga caaggtgtac aaggtatctc
    32821 tgatttgatc acagcgccag gttacgttaa ccttgacttt gctgacgtga aaacagtgat
    32881 ggaaaaccaa ggtacagcat tgatggggat cggtgtggct agtggagaag atcgcgtagt
    32941 agaagcaacg aaaaaagcaa tctcctctcc attgcttgaa acatctattg atggagctga
    33001 acaagtactt cttaacatca caggcggttt ggatatgaca ttgttcgaag cacaagatgc
    33061 ttcagatatc gtagcaaatg cagcaactgg cgatgtaaat atcattctag gtacatctat
    33121 caatgaagaa atgggcgatg aaatccgtgt gacggtcatc gcaacaggta tcgatgaatc
    33181 aaaaaaggaa cgcaagtcta gccgaccagc tagacaagca caaatgcaat cacctgctca
    33241 aaagactgta ttagatatgg atcaagctaa accaacttct tcagaagaag aaaacagttt
    33301 tggcgattgg gatatcagac gtgaacaaaa tgttcgtcct agagttgatg attccaattt
    33361 tgacaatatc gaaaaaaaag aatttgatac attcaaccgc gaagaaacaa aatctcatgg
    33421 cgatgatgaa ttaaatacgc caccgttttt ccgtcgtaaa aaataaagga gaggaagagc
    33481 acatgattgc tgacaacttg cagaaaatca agcaagaaat tcaggattcg tgtgctcttg
    33541 ttcatcgtga tccgaaagaa gttacattag tagctgtaac aaaatctgtt gaatccgctc
    33601 aagctgaaga attggttaga gctggcgtta tgaattgtgc agaaaaccga gtagataaat
    33661 tattagaaaa gaaacaagat ttatttgatt ttcctgaaat aaagtggcat ttaattggta
    33721 atttacagcg cagaaaggta aaattgattg taaatgaaat tgattacttt catgcgttgg
    33781 acagtttaag attagcagaa gaaatccaga agcggttaga gaaagaatta tcctgtttta
    33841 ttgaagtaaa tgtttctggt gaggaatcaa aacatggaat ttcaccagat gagcttttac
    33901 cgtttgtgga atctttagct gcctgcacaa aaatcaaggt tgttgggttg atgactatgg
    33961 cgcctaaaga agcttctgat gagcaaatcc ataaagtgtt tggtacgctg aaagaattgc
    34021 gtgatcttgt tcaagcaaaa ggttatgccc atgcaccatg tagtgaatta agtatgggga
    34081 tgagtcaaga ctttcaaatt gcgattgaag aaggtgcaac atttatccgt gtaggaacag
    34141 cgctattcaa agacgaataa aggaggaaca aaaatgccgc ttttaaacag aggagcaatt
    34201 gctaattttt tcggattagc tgatgaggaa gagtacgaat accaagatta tcctgaaaca
    34261 acaaaaaagg ctgtacagcc aactaaaaaa aatacaacac agaatcaatc aactcattct
    34321 gtatctgctg cgaatactca tgaaaaacaa aaaacggcga cttcacgtcc tgtaacgcat
    34381 gaaagtattc agccggctga aaagaaaaaa gtaattgaac tacatcaatc acctacaaaa
    34441 actgtagcga aagaaagaac agcttcaaga acctctacac aacctaaagc aaatcttgct
    34501 ggaaaaatca ctgtaatgga accaagagct tattctgaag ctatggcgat tgctaaacga
    34561 atcattgctg gagaagcaac cttagtgaat tttcatttag tagaagaaaa acaagcgaga
    34621 agaatcgttg attttctaac aggaacagtt tatgcactag atggagatat caaacgtgtt
    34681 ggagatgaga tttttctttg tacaccgact gggatggaga ttgacagttc aaccgctcaa
    34741 tcttttgcag atgcgaattt gtttgatctg taagaggagg aactacgatt tatactattg
    34801 cactattact tcatttactt caacgggcag cctatttcta tagcattatc ttgatggtat
    34861 atgcattgct ctcttggttt ccaggcgggt atcaatcagg gctaggacga ttattagcta
    34921 agatctgcga accgtatatc agtttgttcg atcgtctgcc tttgaggatt ggacccttgg
    34981 atttcacaat tttaggggca atcctgatct tgaacttagc cgtacaaggt ttagcacaga
    35041 tcgttatatg gataactgtc catttagttt attgattgaa gcaacaaaat ctttattctc
    35101 agaatagata ggaggggaaa attttggatg caaatgtata ccagcacttt cgggcagatg
    35161 aacgtacatt tatcgattcg gtaggcgatt ggattgaaca ggttcaagga caatatgcac
    35221 cctatctgac cgaatttctt gacccaagac aatcctatat tttagaaaca ctgattcgac
    35281 aaagcagtga tttgcgtttc atgttttatg gcggatatga acaggcagaa agaaaaagat
    35341 gtttgatttt ccccgattat tacgaacctg tagaggaaga ttttgaaaca gcattgtatg
    35401 aaatccatta tcctagcaaa tttgccagcc taagtcatgg gaaaatcctt ggaactttag
    35461 tgggaacggg gatcaaacgc tcttactttg gcgatatcat ctcggacggt gatcgctggc
    35521 aagtatttat tgcaaatgag gtggatcatt ttgttgctgc acaagtaaca aagattggaa
    35581 aggttgctgt acgcttggaa ccaatcaaat acacagaaat gattatgccg aaagatagct
    35641 ggacacaaga aagaggaaca gttagttcca tgcgactaga cagtgtgatc tctgagattt
    35701 ataatatttc cagacagcgg tccaagcagc tgatcgaagc tggtaaagta aaagtgaact
    35761 ggacagaaaa tacaagacct gattttgtac ttgaactatt agatattatt tcagtcagag
    35821 gttatggcag agtccagatc caagaacttg aggggaagac gaaaaaagaa aagtacaggg
    35881 tactgttcgg tgtattacgt aaataaaata aaaaggtggt ttactcatgg ctttaactcc
    35941 attagatatc caaaataaga cattcccgac aaaaatgcgg ggatataatc aagacgaagt
    36001 agatgatttc ttagatttag ttgttcgtga ctatgaggaa ttgactcaaa gaaatcgaga
    36061 attagaaaaa gcagtcaaac attctgaaga aaaacttgag tacttcaatg aattaaaaga
    36121 tgctttgaac caatcaatca tcgtagctca agatacggct gataaagtaa aaacaagtgc
    36181 aagcaaggaa tccgaagtga tcgttacttc tgcacaaaac aaagcagatg agttagttgc
    36241 gaacgctgaa aaacgtgcac atcaattaac aactgatgca gaagaaaaag caagaaaaat
    36301 cttgacagat gcaacagaaa aagcaagaca gcttgcgaca gaaactgaag atcttaagaa
    36361 aaaaacacga gtattccatc aacgtatcag cttgatgctt gagtctcaat tagaacaagt
    36421 gaaaagtccg gaatgggatg aaattttaca accattctcc agctacgtga ctgacagcca
    36481 tgaagtgatc aaagaagttt tagcaaaaca gcttgacaat gaaaatgata ctgatgtaaa
    36541 ctcagacaca ggagtcatag aagcacctat gacggcattt gatacacagg ctattgatat
    36601 ctcgatcgtg cctggtgaaa atgaaaacga gtagaacaga aaagcacttt gttattctta
    36661 aagcgagccg atgagagtgg aagttcggta agacactgca tggtgaaaga tcctctactt
    36721 gagacctgct tttccgaaat tagagtaaga agcagcggct gacctcgtta ttggtcacag
    36781 agagtttgca ctagatagat gcaactaaat ttgggtggta ccacggcatg ttcgtccctt
    36841 gcggctgaac atgctttttt gtgtccggaa aacaaataaa ggagtgtatg gttgaatgaa
    36901 aatgaaagaa accttgcaat taggaaaaac aaaattccct atgcgtggta atttgccaaa
    36961 ccgagaaggc gagtggcaaa aagaatggga agaaaatcga atctaccaaa aaagacaaga
    37021 gttgaacgaa ggaaaaccta gctttgttct tcatgatgga cctccatatg ccaatggtaa
    37081 tatccattta ggacattcat tgaataaaat cagtaaagac attatcattc gttcaaaatc
    37141 aatgtctggc tatcgtgcac catatgttcc aggatgggat acacatggat tgccgattga
    37201 acaagtattg gctaataaag gcgtgaaacg taaagaaatg tccatggcag aatacttgga
    37261 aaaatgtcgt gaatatgcac tttcccaagt agataagcaa cgagcagatt tcaaacgcct
    37321 aggagttgca ggagattggg aacatcctta tgttacatta gatccttctt atgaagctgc
    37381 acaagtacgc gtatttggta aaatggctga aaaaggctat atttacaaag gattgaaacc
    37441 aatttactgg tctccgtcaa gtgagtcttc tttagcggaa gctgaaattg aatataaaga
    37501 tgtaaaatct ccatctattt ttgtagcttt caatgtcaaa gacggcaaag atattcttga
    37561 tgaagataca gcttttgtca tttggacgac aacaccatgg acattgccag caaaccaagg
    37621 tattgctgta aatccaaaat ttacgtatgt attggtggaa gccgatggcc gtaagtttgt
    37681 cgtagcgaaa gatttgctag aaacagtcca atcagaaatc ggttgggaaa atgtaacagt
    37741 tctaaaagaa tttgctggac aagaaatgga atatatgact gcaagtcatc cattctatga
    37801 acgtgaatca cttgtgattt taggcgatca tgttacttta gacgctggta ctggattagt
    37861 ccatactgct ccaggacatg gggaagatga ctatatcgct ggtagcaaat ataaactatc
    37921 tgtagtgtca cctgtagact caaaaggatt gtttacagaa gaagcacctg gatttgaagg
    37981 tatcttttat gacaaagcaa atcctatgat cactgatcta ttaaaagaaa aaggagcttt
    38041 gctgaagcta gatttcttta ctcatagtta tcctcacgat tggcggacga aaaagcctgt
    38101 gatctatcgg gcaactccac aatggtttgc atctatcgat aaattccgtc agaatatttt
    38161 ggatgaagta gaaaaagtag agtgggttat cccatggggt aaaacacgtt tgtataatat
    38221 gatccgtgac cgcggcgatt gggtaatttc ccgtcagcgt gcatggggag taccacttcc
    38281 gattttctat gctgaaaatg gagaagcaat catcacacca gaaacaatcg aacatgtggc
    38341 aaaattattt gctgaacatg gatcaattat ctggtttgaa aaagaagcga aagaacttct
    38401 tcctgaagga tttacccacc caggttctcc taatggcgaa tttacaaaag aaaaagatat
    38461 catggatgtc tggtttgatt ctggttcttc tcatgaagct gttttacgtc agcgaccaga
    38521 attatcattc ccagcagata tgtatttaga aggatcagac caatatcgtg gctggttcaa
    38581 ctcaagtatc acgactagtg tggctatcaa tggtgtagca ccatacaaat cagttatttc
    38641 tcaaggattt gctttagatg gtgaaggccg taagatgagt aaatctttag gaaacatcat
    38701 tgcaccagaa aaagtgatca aacaatttgg tgcagatatc cttcgcttat gggtatctag
    38761 tgtagattat gaagcagatg ttcgtgtttc tatggatatc ttaagtcaag tggcagaagt
    38821 atatcgtaaa atcagaaata cgatgcgttt cttattagcg aatacagaag atttcgatcc
    38881 taaaaaagat actgtttcgt acaatgattt acgttctgta gacaaatata tgaccgttcg
    38941 attgaaccaa gtaatcaaag aaatccgaga agaagggtac gacaaatata acttcatgca
    39001 tatttaccgt acagttatga acttcttgac ggttgatcta tcgtcattct acctggattt
    39061 tgctaaagat gtagtttata ttgaagcaga agatgattat caacgtcgtt gcatgcaaac
    39121 tgttttctat caaacagctg tagctttgac aaaattattg actcctatca ttccgcatac
    39181 agcagaagaa atctggtcat tcctgaaaga agaagaggag tacgttcagt taagcgagtt
    39241 tcctggttat gaagagtttg caaaccaaga agaattgatg gatacctgga ctgctttcat
    39301 ggacttccgt gataatgtct taaaagcatt ggaagaagca agaaacagca aattgattgg
    39361 taaatctttg gaagcaaaac taactgttta tccaaaacaa caagttcgtg aaatgttaaa
    39421 agctttggat gcagatattg cacaattatt gatcgtttca ccagactact ttgaagttat
    39481 ggcagcagat gaacaagtgc cagataacgc tatggtattt gatgatgttg ctattacagt
    39541 ggaaaaagca gacggggaaa cttgcgatcg ctgtcgccaa gttcgtaaag atgtaggagt
    39601 agatgaaaaa cttcctcatc tatgcggtcg ttgtgcaaaa atcgttgaag acttctatcc
    39661 tgaagctgta gctgaaggat tcgaagaaaa gtaaaaggga aaagggggtg tgacatgttc
    39721 tttgtcacac ccccttttgt tatcaatcgt ttaattttcc acgttcacga taccagttat
    39781 ctggttgcca gatccattcg aatccgtctt tttcaagtag ttcgaaagct gctttaggtc
    39841 ccattgatcc agctttgtag ttgggaaaat ccggttgtgt ttggtcccaa gcttttcgaa
    39901 tgacatccac gatatgccaa gattgagcga cttcttccca atgagagaag ttcgttccat
    39961 caccattcaa cgcatctaat agtaattttt cgtaagcttc tgggctattt tcaaccattt
    40021 ctgcactatt tctaaattcc aatttgattg gttccgtttc aaatccttgc cctacttctt
    40081 tcccattcaa ggataaagaa aatccttcag ttggctgaat atagatcgtt agaacattag
    40141 gtggaagtgt cgtatcgtca catggttcat cgacagatgt tttgaataca ttaacaggaa
    40201 cttgtttgaa tacgatgttg atccgagtcc ctttttcagt caaacgtttg cctgtacgta
    40261 catagaaagg aacacctgac caacggaagt tatcgatagt gaatttgcct gcgacgaatg
    40321 tttcagtctc tgattgagca tccactttgt cttcttcgcg atagcccttg aattgtacac
    40381 catccaattc accttgagca tattgcccgc gaacgaagtt ttctaacact tcttcttcgt
    40441 tataaatgcg gatagctttc aatgctttga tcttttctgt acggatttct ttttcggaaa
    40501 atgctactgg cggttccatt gcaagtaatg ctacaacctg taaaatatga ttttgcacca
    40561 tgtctttaag tgcgccacta tgatcgtagt agcccccgcg atcttctaca ccgaggtttt
    40621 cgccaaaagt aatttggata ttgtcgatat agcgattgtt ccattgagcc tcaaaaatat
    40681 tattggcaaa acggatagct gagatgtttt ggatcatttc ttttcctaaa taatgatcaa
    40741 tcctgaaaat atcttgttct gggaatactt ctctgatttg ctggttcaat tcaaaagcag
    40801 actggtaatc agaaccgaac ggtttttcta tgatcaaacg attgaagcca ttttttgtca
    40861 taatattttg tgatttcaaa tgagaaacga tcgttccaaa aaattgaggt gccatagcca
    40921 aataatagac acggttaccg tttaattgat agtgtccatc taatcggtca gacaattctt
    40981 ttaatgtctg ataatgcgtt gaatcattta catcatggga ttgatagtaa aaatggcttg
    41041 caaaggtagt tgcttcttca tctgaaggat tcaatgcacg gattgtttct ttgacaactt
    41101 cacggtagtg ctcatcgctc cagggacggc gagcggttcc gattacagca aaatgctcgc
    41161 ctaaatttcc ttttctgtaa aggcggaaca aagacgggta caatttacgc tgggctaggt
    41221 cacctgtacc gccgaatatc gtaaacaata catttttttc attagacatg tggatacact
    41281 cctttatatt ttatcgacaa aaatcgtgct ggccgcctta aagctgacgg ggattttctt
    41341 tctatcattt tggatagtga tcggtccttc ataagctgcg atgtcaacga tctcatattc
    41401 ttcgttgata ttcaggtcaa tagtgactaa gtaatccaac aattcttttt catcaagcac
    41461 tcgtgcgata cgcacttttg taccgacagg ataggactcc aatgtttgtc ggcgttcttc
    41521 atggacaact tcctcttttt taggaatcga tcctccatga ggacaatgtt caggatgatt
    41581 caaatagttt tctaaatgat ctgcaagatg ttccgaagtc acatgttcca atacttcagc
    41641 atcatcatgg acttcattcc agctatagtc taaatgttca actaaaaaaa cttcccataa
    41701 gcgatgtttg cgaatcagac tgctagcttt ttgcagtccg cttgcagtca attgtacacc
    41761 ttgataaggg gaatgttcta caagctcttc tttcacaagt ttagaaatca tttcactgac
    41821 agatgcagct gagacatcta gactagatac gatttgttta ttgttgactt tcgttgtatc
    41881 tccacctagt tctaaaatga ttttcaaata atcttctcga tttggtgtca tattcgatca
    41941 tcctttcatg gtaaaaaacc atcattagta agtgtaacaa aaaaaacgtt aattgactat
    42001 ttttctaaat aaaaaacaaa ggatttttag acgtttttac aacaaaaaac gaaagatgat
    42061 agagaagact cattctttcg tttataaagg atatatatat acattgggag ggatctattt
    42121 ttcagataac aacttatatg aagtgtctgc agattcgtca taaagagttt gttcgggata
    42181 agaaaagata agataaaaac catactgatc acgtacgact aaacgatatt catctatttc
    42241 taccacagat gtgccaagag actgaccgtt gatcaaaaac gtgtaatcag atgtaatctg
    42301 taattgttgt ttttgctggc tatcactaaa gaaccatgta ccttgatact ttgagagtcc
    42361 ttctgacttt tccgcttgtt ttttcttttt caagtaaata aaacttgaga gaacggtaat
    42421 gagtgaacca agaaaataga tcattttttt tatcaccaaa tcactccttt atgagaaaat
    42481 aatcgcatag acatattctt tcttctttat ttcattgtac cgaataaaag ctaaaatttc
    42541 tctaagactt tcgactggtt tttttactgt ttttctacaa attatgatag gatacatagt
    42601 aggaagaaag gtgacataat ttgaagtatc agcaagtgga aatagctttt tttattgacc
    42661 gcccgaatcg gtttatagca cattgtatga aaaaagacgg agaaataatt attgcgcatg
    42721 tcaaaaatac aggtagagga aaagaggtcc tgataccagg cgctgaagtg gctattgtat
    42781 ttgctcctgg acctaaacgg aaaaccgcct atgatctgat tgctgtaaaa aagaagggtg
    42841 actggtttaa tatcgatagt caattgccta atcggctggc aattgatggt attttagacg
    42901 gcacgatcct tttaccagga ttaactggtc catttacggt atacaagcgt gaagtaactt
    42961 atcttcactc caaatttgat atttatttag agacggtggt cggacaaaaa gcatttgttg
    43021 aagtcaaagg tatgacactt gaaaataaag cgattggtgc ttttcctgat gcgcccacac
    43081 tcagaggatt gaaacatata ggagaattaa cgtatgccgc acaagatggt tatgccgctt
    43141 atgtcttgtt tattgctcag ttcgaacatc ttcatctggc aacgatccat gaagaaatgc
    43201 aaccagcact agctgatatg gttcgacatg cgcagcaaag cggagttcaa atcctcgcct
    43261 ataattgtca agtatcggca gatgaggtaa ctgtcaagca ggccattcca tttgatgtag
    43321 atatcccttt tgaagatcct atacaataga aagaaggtaa aataatgatc aacttaggta
    43381 ttatcggaac aaattggatt actcatcaat ttgtccaagc agctttaagc acaaaaaaat
    43441 atgaattgac agcggtatat tctagacatt tggaaaaagc acaggaattt ggttctcatt
    43501 atgaagggga tatcgaattc gcaacagatt tgaagacttt tttcaatatt actcatatgg
    43561 acaccattta tattgcttct ccgaatagtc ttcactttag tcaagctaag caagcaattt
    43621 tggcaggaaa aaatgtgata gttgaaaagc cagctttttc gacgccaaaa gaaatggatg
    43681 aaatcatcca tttggcaaat gaacacaatg tgtatttttt tgaagcagca agaaatatcc
    43741 atgaatcagg ttttaaaaag attgccgatt acttaccatt aaaagacgag ataatcggtg
    43801 caaatttcag ctatatgaaa tattcttcgc gctatgacca agtattagat ggagaagaac
    43861 cgaatatctt ctcgactcat ttctcaggtg gtgcattgat ggatttaggt gtttaccttg
    43921 tatatgcagc tgttgcttgg tttggaatgc caaaagaagt ccattatttc ccaagaaaga
    43981 tttctactgg tgtagatggt cttggatggg ggatcttacg ttatgagaat tttgatgtat
    44041 cgatccagcc aggtaaaatc ggtgattctt atttaccgtc agagatttat tttgactcag
    44101 gaacgctagt gatgaatgga gtcaatgcaa ttgaacaagc acaatttcat gatcgcgcgc
    44161 ataaagaaat cattgatttg gcggtaacag ctgcagaaaa tccaatggca gatgaagcag
    44221 aggattttgc caatgtcatg gctcatccga aagatccagc ttggggattg ttgtatgaag
    44281 agtggatcga gctagctaga aatgtgaacc aagtgattta tgatttaaga aaaaatggcg
    44341 gcatcaaatt cgatgcagat aatgaggaag atttttaatg aacgaatttg tacaagtatt
    44401 tcccgaaaat tggcagaaaa aatatgagga taaagggttt atctcacctt ctcttattca
    44461 acgagcagtt tttcagccac taagtaataa acaaagtata gtagcggttt ctcctacagg
    44521 ttcaggtaaa acactggctt atctttggcc tctactacta aacgtggaac caggtgaagc
    44581 aagtgcgttg gtcatttttg cttcttctca agaactagct attcaagtag cagatgttgc
    44641 acgtgaatgg ggaaaagaca aagagttgaa agtccaatct ttggtgggcg gagccaacgt
    44701 caaacgtcag attgagggtt tgaaggaaaa accagaaatc ttgattggta caccaggaag
    44761 aatattggaa ttgatgaaag caaaaaaaat aaaagcacat caagtgaaaa caatggtatt
    44821 tgatgaagca gaccagctat ttgacgctgg gaacagccaa atcattgatc aaattcttca
    44881 tcaagctcca acagagtatc agctagcttt tttttcagcg acagcagatc gttcccttga
    44941 aagcattgaa aaaatcactg ggaaaacgtt tgagacaatt gacgtgacag cagaggacga
    45001 ttctcgaaaa ggactgagac attacttttt acgtgtaccg atacgaaaga aagatgacta
    45061 cttgagacgt ctcacgcata tagaagattt tcatggtctg attttcttta atcaactgac
    45121 tgaattaggc gtaatggaag aaaaattgct gtatagaggt gtaccggtag gaagtctggc
    45181 ctctgatcag aacaagctac tccgaaaagc agcgctagaa caattcaaaa agaaaatggt
    45241 cagagcactt ttgaccacag atgtggcggc tagaggttta gatattacag gtcttcctta
    45301 tgtagtgaat gcagaagttc cgttaagtga agaagcttat ttgcatcgtt cgggaagaac
    45361 tggtcgaatg ggtaatgaag gaatagtaat tacactcgta caagataata atctaagaga
    45421 tttaaaacgg cttttacgtc catcaaatat aacacttgaa gagatttttt tatacggagg
    45481 taagctgcaa actgagcagc caaccaaaga agatgcagct cctgcagtaa ataaaaagta
    45541 tgcgaaaact ttttctggaa aggagcagaa cccagaatat aacaaagaaa aaataagaaa
    45601 aaaagttaaa aataaatcaa aaaaagagag aaataaagga gcaaggcgca aataagcgcc
    45661 tttttatgct atgattatga aaacgggaac aaaattggtt gccagaggag gggttatgtg
    45721 aaagaacgtc aagaagagat cttgcagcta ttgacagaaa accaacaagg attgacagca
    45781 agtgatgtag ctgagcgctt aactatcgat cgaagcaatg caagccgata tttaagtgaa
    45841 ctatacaagg cccatcatat tgtaaaaacc gctggccgac cagtcgttta ctctttgcca
    45901 acggaaaagt caaaatctga tgaagtgcat gtcgactctt ctacgcaagt gacttttgaa
    45961 acactcgttg gagaaaatga ttctttaaaa gtcagcatac agcaggcaaa agctgcgatt
    46021 ctttatccac cgcgggggtt gcatacgatt atttttggag aaactggtac agggaagtcg
    46081 atgtttgctg aatgtatgta tcattttgca atcgattcag agatgctttc tgcagatgcg
    46141 ccatttgttt ctttcaattg tgcggattat gctcaaaatc cgcaactgct ctttggacac
    46201 atctttggga tcaaaaaagg ggcttacaca ggagcagctc aagatagtcc cggtctaata
    46261 gccaaagcag acggcggtat attgttttta gatgagatcc accgtttacc gccggaaggg
    46321 caggaaatgc tgtttacttt cattgataaa ggtatttacc gtccgcttgg agaaagtgca
    46381 caagtacatg aagcatctgt tcagatcatt ggggcgacga ctgaatcttc agaaagtttc
    46441 ttatctacgt tcaatcgacg gattcctatg gcaatcacct tgccgaatct tgcttcacgt
    46501 tcactggatg agcgttatga aatcatctct ctttttatca aacaagaagc gaatcgtttg
    46561 aatcaacgta tcgatatcga aaaagaagcg attcttgcct tcatgcttta tgatgcggaa
    46621 ggaaatatcg gtcaagtgaa acgagacttg aaactagtct gtgcaaaatc ttttttacat
    46681 taccgtactc atcatgagaa aaagctaatc attcgaaaag aagaactttc ccttcaagta
    46741 caaaaaggat tactgaaaat caaggaaata ccggaacgtc tcgaccgatt catggacagc
    46801 aaaagtcagt atttgacatt tgaaccagga tttgctgatg ttgtctggtc acaagatcca
    46861 gaacgaaaca tgcaggtcta taacgatatt gaggaaaaag tcacttctct ttccgaaacc
    46921 ggtgtggaga acattgatct agagaccctc atttcaaaag atgtggatgc ttatttccag
    46981 acatatgtca aagaattgac taaaagttcg attcccaaag atcttttgcc agatgaaatc
    47041 tggcggttaa ccaatcaatt gtatgatgtg gctgaaaaac ggttagaccg tatttataat
    47101 gaaaaagctc gttttgcttt tgctttgcat ctgcaaagta cattggatcg tgtgagagaa
    47161 ggtcatatga tcgtccaccc agatttgaat aacgtacgga aaaattttaa gagggaattc
    47221 caggtagcaa tcgatctttc aacgatcatt gaagaagaag aacaagttga gattcctttt
    47281 gatgaaatcg gctttattag tatgtttctt tctatcaatc taggagaaag tgagcctgtt
    47341 aaagaaaaca aagtagatgt tctcatattg atgcacggca gacagactgc ttcaagcatg
    47401 ttggagatgg cacaagaact attaggaaca aaagtaggga aagcttttaa tatgccactg
    47461 gttatggaag ttcaaacaat gtatgaagaa gtgttacaat atgtcaaaaa ctatcaagag
    47521 catttgacga atggcctact cttgttaaca gatatgggat cattaaacac cttcagtgat
    47581 ttgatttatg aagagactgg cattcggacg aaagctatca cgatgacaag taccatgatt
    47641 gtgatagaag cgatacggat ggcaagtgtt ggaagaagct tagaagatat ttatcaaaat
    47701 atccagcttt cctttgaatc gatcgtacgt gatcagttca aagcggaaaa gtctgaaact
    47761 ttacagcgta aaaaagccgt aatcgtgaca tgctttactg gtgaaggagt agctgccaaa
    47821 ttatatcagc gtattatgcc agtgattaat caaaataagg tagaactgat ccagatgcag
    47881 tttttagaac gtgaatcatt caaaaaacac atcgatgcct tattggaaga atatgagatc
    47941 aaagcaattg cgggaacagt tgatgttgat tatcaaaata tcccattttt ttcagcctat
    48001 gacgtttttg atgatgaaaa actaaatatt ttaaagcgta tagctgatga tgagatcccg
    48061 acagctaaaa ttgtcgattc acttgctgga tcattaagcg atgtcttttc tattgatgaa
    48121 ctgatccgtt cattgcaaaa gacagttcag cagatccaga ccgatttgtc gattatcgtg
    48181 gaacctggag tagatactgg aatcgttatc catttagcct tcatgttaga cggattgatc
    48241 aaacaagaaa gccatcggca atttgagaaa ctgtctattt tctcaaaaat ttatcgtttg
    48301 gaaatggata tcgttcgggc aaacttgatg gcactagaac gaaaatatca ggtaaaaatt
    48361 ccagaagatg atatcgcatt tcttacacaa atgtttcttg aaaataaaat aaaaaaatga
    48421 acacactaat catactgttt acacactaaa catctgtgtg cgcactataa aaaagagcta
    48481 aaaagcatga aaacgctatt tcagctcttt tttttattgg catgcttctt gcgttatata
    48541 aaagtgtaaa aaaataaaag gagtgttaaa aatggcaaaa aaaacaatca tgttagtttg
    48601 ttcagcaggg atgagtacga gtttattggt cacaaaaatg caaaaagcag cagaagaaaa
    48661 agggatggaa gccgatattt ttgcggtctc tgcctcagat gcagataata atttagaatc
    48721 taagaatgtc gatgtattat tattaggtcc tcaagtacgt ttcatgaaag ctcaatttga
    48781 acaaaaactt gcgcctaaag gtattccatt agatgtcatc aatatgcaag actatggcat
    48841 gatgaacgga gaaaaagttc ttgcacaagc agaaaatcta atgaaataaa tcaagtatgc
    48901 agttgggggt ataacaacgt ggaagatcaa caaaatctag aagccatcat ggggttaatc
    48961 atgtttggcg gtaatgcaaa gagcgatgca atggaagcga ttgcggctgc taaaatcggt
    49021 gattttgagt tagcggatag gaaaatcgct gatgcagaag aatcacttat acaagcgcat
    49081 catgcacaaa cagacatgtt gacacaagaa gcacaaggaa atcatatgca agtgacatta
    49141 ttgactgttc acagtcaaga tcacttaatg acatctattg cgtttactga tttagcaaaa
    49201 gaaatcatcg aactttatcg tcgcgtatac agcgaataaa tacaacttct tttttgaacg
    49261 gaagaagaaa aatttttgaa cggatttttt attttgttat aaggagaact ctctttatat
    49321 aaaaaactta taggaggaag taaaaaatga acggactatc aagttggatg gagaaataca
    49381 tcctgccggt tgccgcaaaa atcggagcgc aaaaacattt agttgctttg cgtgatgctt
    49441 ttatcggcat actgcccgca acaatggcgg gatcagtcgc tgtaatgatc aatgcaatta
    49501 ttcgtgattt accgcaacag tttaacagta cctatgctga cagtttagca gcagacaaag
    49561 gaatctttgc cgtattgaat gtcatcatcg gaatcaatgg ttttgtctgg aacggtacac
    49621 tagcaatcgc cggattgatc tttgcctttt cttgggggta taatcttgcc cgagcttatg
    49681 gagtaaatga tctagctggg gggatcgttg gtttagctac cttgattcaa ggtatttcct
    49741 ttggtccagc attatcagca gcattagatg tgaatgttcc ttcaaatgtc gcagatgcaa
    49801 tcaatagttc tgagattggt gcaacgattg cagatggcac actgtcagtt ggattatggg
    49861 gttggctaaa actagatcat ttgaatggaa acgcttattt tacagttatg atcatgggag
    49921 ctatttcagt cattattttt tgtaaattga tgcttgccaa cattacgatc aaaatgcctg
    49981 attcagtacc accagcagta tctaaagcat ttgcagccat tttaccagcg acgatcgctt
    50041 tatacgtcat tgctattatc aattatatcg taggtcgtgt agctgatggg caactgatca
    50101 ttgatttggt gcaaaaatat attgcagaac cattcctagg attatctcaa gggttgggag
    50161 cagtattgat cgtcacagta tttgttcaag tattctggtt ctttggtatt catggatcaa
    50221 atgtattggc accagtgcta gagggaatct ggggacaagc acaactaatc aatattgata
    50281 ttttccaaaa aggttataac ggaaaaactg gtacaccagc cgtgttagat gcaatcgaag
    50341 atggaaaagc ttatatgtgg gtccgcggat cgttcgatgc ttttgcatgg tttggcggat
    50401 caggcggaac gatcgtctta ctgatagcta tcttgatttt ctctaaacgt gcagattatc
    50461 taacagtagc taaattatcc attgggcctg gtatcttcaa tatcaacgaa ccagtgatgt
    50521 ttggtctgcc aatcgtattg aatgcaatca tgtttatccc attcttgatt gctccagtag
    50581 tagcaacagc aattggatgg acagctactt atcttggcct agtcgctccg gtatcgcagc
    50641 aagtcacatg ggtagtaccg ccgttcttat tgtcattctt agcaacagga gcggattggc
    50701 gggcaccgat cgtaacatta gtatgtatgg tcgtgacttt cttgatttgg gcaccatttg
    50761 tcatctctgc aaacaaaatg gaccctgccg caggcgaaca acaataagat gaacgtgagg
    50821 agcgggacgc tttaagtttc gttcctcata atgaattttc gaggaggaac tgaaatgatt
    50881 acaattgcca gtgcaatgca acaagatgaa atcaaagaat tgttagaagg atacaatgaa
    50941 gaaggaatga cttttacatt taaagaaaaa caaggaatca agctgttctt tgaaacaagt
    51001 gcagaagatc cggaagttgc ggctaaaaaa gcaaaggaac taatcaaagc acagccttgg
    51061 ggaacagtcc tttattttca atcaacagct gtttagaaaa tggtttaaaa agataggatg
    51121 gttgagatga taaaaaacgg tttattttta tgtagtattg gttctttgat cgtgttgtat
    51181 agtttgaatc cacattcgca aaggtatgat ttgtatagta tggtcacagg tctttttcta
    51241 acaggattag gaatctattt cttcatgaag gggaaaaaaa gagaagaagc aaaggagaaa
    51301 aataactaat ggaaatcacg aaaaaattaa atattccagc atcgtttttt tacgataaag
    51361 ttatggactc tgtccttttc gatgtacgta aagcgaccgg caaatcagta actagaaaac
    51421 agctgaaaaa tttggagtac gtcaaacaat tctcgcaaaa taaccgggct cgcataaaaa
    51481 tcgaagaagt cattgaaaac cagtcttata agtttcgtac atcgacaacg aagaatgaat
    51541 tcgtcgttca ttatcaaatc aaaccattgg acgattcgac ctgtgaggtt cattacacag
    51601 aaaaaatgga gtcttatggc atgcttcaaa agttgaatga tatgctttta ggaacaatct
    51661 tgatgtattt taaacggaga cgtttcaaaa aaatgctaga tatgatcgaa gcaacgtatt
    51721 aaatcaatga aacatggtac tatttagtta ttgaaaaatg aagtaagcta gaaggaattt
    51781 ttcataggaa gatagatctt tcctgcgaat cctcactagc ttttttgtag gaaggggcag
    51841 caaaatgaca aacaaagtaa gagagctagg tgtatcttcg attgaggaaa tgtttgattt
    51901 agccacttat gcatttaatg gggcagatac acctgaaagg cgagaacgtt ttcgtacact
    51961 tgcggagaat tcttggaatt acggattttt tgatgaggag gatcggctaa caagtcaagt
    52021 catggcgacc ccttttccag tgaattttta cggacaagag tacctaatgg ccggcattgg
    52081 ctatgttgct tcttatccag aagcaagggg acaaggcggg atcaatcaga tcatggaaaa
    52141 gatccttgaa gattgccgtg atcgtaaagt aagcttatct tatctggcgc cattctccta
    52201 tcccttctat cggcgctatg gctatgaaca atcatttgat aaaatcagtt atcaactaaa
    52261 cagtcgtgat gtgccgttta tcaaaaaaaa atctggaaaa ataaagcgta tggattttga
    52321 agatgcaaaa gctgcaatca gaagaattta tgaacagatg ccggaaaacc aacgagctgg
    52381 tctgaagcgc gaagactggt ggtatacgta taaattcaag caaaaaggca attaccagtt
    52441 tgctttgcat tttgatgata atgaggaagc aacgggatat atcgtttatc agcttgcaac
    52501 gtcttccttt attattgcgg agtggggatt tttggatcat gatgcttttt gcagccttgt
    52561 tcgcttcgta agttctcaca atggtgcttt tgaaacattt gaatattcat gcggctacgg
    52621 cggtaatgat caaaattatc tattagaaaa tccatttgct tctgtaatga tcacacctta
    52681 tatgatggcg aggatcgtag atatttcttt gtttttggaa aattatccat ttaaaaagag
    52741 acaagctgca tttgccctac aaatagaaga agatcattat gcgaaatgga atgaaggaat
    52801 cgtggaagta acaatcgaaa atggaaaaaa tcatctctca aaagtagaac aaacaaaact
    52861 cccaatcgtt cgaggaagta tccaacatat cacgcagcta ttattagggt atcgtaaagt
    52921 ggaagagttg gtattccaag aaagaataca ggtgacaaaa gaatatgaag aaacgttaag
    52981 ctcaatcttg ccacagcaac agccaattct taatgactat ttttgataaa aaacgggtga
    53041 tgacaatatt tttgtcacca cccttttgtt tttcatttgg catttgaagg aaaatgttaa
    53101 agtaaatcct cttttttcgt aaatttgaat tttttgtttt cttttgtttt agggttttct
    53161 tttttgtggt cttggattgc ttctttcgta atcttttccc gatttttttt agatgaatct
    53221 aagcgttctg cgtatacttt ttcttttggc ttatcattca ttggaaagga cctccctaca
    53281 ttatttcatt ctacctctag tttatcatct gttaaggact agtggaaacg ttttgcctaa
    53341 cttataagct gattttttta gcagataagc ttgcaagtaa aaaacaaaca agctacaatt
    53401 gatttattga aattgttcct gtagctcagt aggatagagc aatcgcctcc taagcgatag
    53461 gtcgctggtt caattccagt caggaacgta taaataaccc ctgtatcctt tgcggtacag
    53521 gggtttgttt tgtatttgtg ccaaaaatgt gccaaactag ttatcatttt tatataaact
    53581 atcgaacaca gtattgattt tttctttatc ctgatcttca agctctcgaa caatatgaag
    53641 ataag
//
LOCUS       DANKKG010000007        44818 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000007 DANKKG010000000
VERSION     DANKKG010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.007"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(2..1012)
                     /locus_tag="PW004_000339"
     CDS             complement(2..1012)
                     /locus_tag="PW004_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL2725338.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(1192..1536)
                     /locus_tag="PW004_000340"
     CDS             complement(1192..1536)
                     /locus_tag="PW004_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDL2725339.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(1529..1963)
                     /locus_tag="PW004_000341"
     CDS             complement(1529..1963)
                     /locus_tag="PW004_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HDL2725340.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            2101..2838
                     /locus_tag="PW004_000342"
     CDS             2101..2838
                     /locus_tag="PW004_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2725341.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            2831..4042
                     /locus_tag="PW004_000343"
     CDS             2831..4042
                     /locus_tag="PW004_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2725342.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            4246..5028
                     /locus_tag="PW004_000344"
     CDS             4246..5028
                     /locus_tag="PW004_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HDL2725343.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            5098..5760
                     /gene="trmB"
                     /locus_tag="PW004_000345"
     CDS             5098..5760
                     /gene="trmB"
                     /locus_tag="PW004_000345"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HDL2725344.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(5858..7612)
                     /gene="ade"
                     /locus_tag="PW004_000346"
     CDS             complement(5858..7612)
                     /gene="ade"
                     /locus_tag="PW004_000346"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HDL2725345.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNNQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            7744..8673
                     /gene="tadA"
                     /locus_tag="PW004_000347"
     CDS             7744..8673
                     /gene="tadA"
                     /locus_tag="PW004_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HDL2725346.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            8630..9679
                     /locus_tag="PW004_000348"
     CDS             8630..9679
                     /locus_tag="PW004_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HDL2725347.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            9676..9954
                     /locus_tag="PW004_000349"
     CDS             9676..9954
                     /locus_tag="PW004_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDL2725348.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            9929..10399
                     /locus_tag="PW004_000350"
     CDS             9929..10399
                     /locus_tag="PW004_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HDL2725349.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            10416..10718
                     /locus_tag="PW004_000351"
     CDS             10416..10718
                     /locus_tag="PW004_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725350.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            10735..11106
                     /locus_tag="PW004_000352"
     CDS             10735..11106
                     /locus_tag="PW004_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HDL2725351.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            11078..11437
                     /locus_tag="PW004_000353"
     CDS             11078..11437
                     /locus_tag="PW004_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725352.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            11609..12616
                     /locus_tag="PW004_000354"
     CDS             11609..12616
                     /locus_tag="PW004_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL2725353.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            12641..13825
                     /locus_tag="PW004_000355"
     CDS             12641..13825
                     /locus_tag="PW004_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HDL2725354.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(14262..15845)
                     /locus_tag="PW004_000356"
     CDS             complement(14262..15845)
                     /locus_tag="PW004_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDL2725355.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIVLVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(16423..16566)
                     /locus_tag="PW004_000357"
     CDS             complement(16423..16566)
                     /locus_tag="PW004_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HDL2725356.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(16789..17268)
                     /locus_tag="PW004_000358"
     CDS             complement(16789..17268)
                     /locus_tag="PW004_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL2725357.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(17341..17625)
                     /locus_tag="PW004_000359"
     CDS             complement(17341..17625)
                     /locus_tag="PW004_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725358.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(17703..17897)
                     /gene="ssrS"
                     /locus_tag="PW004_000360"
     ncRNA           complement(17703..17897)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PW004_000360"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(17969..19255)
                     /locus_tag="PW004_000361"
     CDS             complement(17969..19255)
                     /locus_tag="PW004_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HDL2725359.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            19393..20064
                     /locus_tag="PW004_000362"
     CDS             19393..20064
                     /locus_tag="PW004_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HDL2725360.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            20030..20518
                     /locus_tag="PW004_000363"
     CDS             20030..20518
                     /locus_tag="PW004_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HDL2725361.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            20531..21478
                     /gene="prmA"
                     /locus_tag="PW004_000364"
     CDS             20531..21478
                     /gene="prmA"
                     /locus_tag="PW004_000364"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HDL2725362.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            21479..22243
                     /locus_tag="PW004_000365"
     CDS             21479..22243
                     /locus_tag="PW004_000365"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HDL2725363.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            22476..24689
                     /locus_tag="PW004_000366"
     CDS             22476..24689
                     /locus_tag="PW004_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HDL2725364.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            24700..25146
                     /locus_tag="PW004_000367"
     CDS             24700..25146
                     /locus_tag="PW004_000367"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HDL2725365.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            25317..26576
                     /locus_tag="PW004_000368"
     CDS             25317..26576
                     /locus_tag="PW004_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HDL2725366.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(26665..27612)
                     /locus_tag="PW004_000369"
     CDS             complement(26665..27612)
                     /locus_tag="PW004_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL2725367.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            28006..29298
                     /locus_tag="PW004_000370"
     CDS             28006..29298
                     /locus_tag="PW004_000370"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HDL2725368.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            29313..31082
                     /gene="aspS"
                     /locus_tag="PW004_000371"
     CDS             29313..31082
                     /gene="aspS"
                     /locus_tag="PW004_000371"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HDL2725369.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            31191..32066
                     /locus_tag="PW004_000372"
     CDS             31191..32066
                     /locus_tag="PW004_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL2725370.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            32332..34215
                     /locus_tag="PW004_000373"
     CDS             32332..34215
                     /locus_tag="PW004_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDL2725371.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(34290..34574)
                     /locus_tag="PW004_000374"
     CDS             complement(34290..34574)
                     /locus_tag="PW004_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDL2725372.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(34564..35298)
                     /locus_tag="PW004_000375"
     CDS             complement(34564..35298)
                     /locus_tag="PW004_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HDL2725373.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            35427..36050
                     /locus_tag="PW004_000376"
     CDS             35427..36050
                     /locus_tag="PW004_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HDL2725374.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            36242..37363
                     /locus_tag="PW004_000377"
     CDS             36242..37363
                     /locus_tag="PW004_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HDL2725375.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            37370..40498
                     /locus_tag="PW004_000378"
     CDS             37370..40498
                     /locus_tag="PW004_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HDL2725376.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            40517..43711
                     /locus_tag="PW004_000379"
     CDS             40517..43711
                     /locus_tag="PW004_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HDL2725377.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(43857..44564)
                     /locus_tag="PW004_000380"
     CDS             complement(43857..44564)
                     /locus_tag="PW004_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDL2725378.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        14378 a         7961 c         9405 g        13074 t
ORIGIN      
        1 tttattttga tgaatcagtt gtttcttcag ttgaagattc ttttgttgaa tcagttgatt
       61 ttgtgtcaga actctttgtt gaagctgtag tttcacttga gtctttcgta cttgatgaag
      121 ttgttgtgaa tgcactcaac acattttcaa aggcatcatc tttgattttt acatttgcat
      181 cttttaattc ttcaccgatt actttagttg tgaatgcatt gtcacttagt ttagtttcag
      241 tagcgatatc ttttagttga tctttgtatt tatccatgtc attgccttta ttttggtttt
      301 tcaccatttt gacaacgtag tattctgtcg cataagaagt aggatttgtt gtagtaataa
      361 catcggagat ctcaccgtct ttcaatttga aagctgcttc ttttacttct gcaggaatag
      421 ttgttgtcgt tgagtcgaat ttaactttac cgccatcttc ttttgtctct gtatcagttg
      481 atttgtcttt ggctaattta gagaaatcat ctccatcatc cgctgatttt ttcacatctt
      541 ttgcttcatc ttcacttgat aatttgatga tttgtgcttc tacttctgga tggaaagatt
      601 tccaagcagt tttcaaatcg tcatctgtaa tatctacgtg agctttcaag ccagcttcaa
      661 aagctaaatt gttacgaata tattctttat aagtatcttt cgtatatcca gcagcttcta
      721 attgtgattc aaaagtatca cctaatgatt tcgcttgttt gtcgtattct gcatccactt
      781 gcttatcggt tactttatcg ccatatttgt tattgaatac tttataaatg atcatccgtt
      841 gtactaagga ttggttcgat gattcaagtt ttgcttcatc atagaaatca gatacagtga
      901 tcgttccgcc tttcatcgtt gcgatatctt tattagtatc gcctgaacaa gctgctagag
      961 ttaaaacagc caacgcacta gtagctgcca aaataattga tttttttttc attaaaattt
     1021 gcactcctgt aattagttat tatttttata tattaactat accatatccc atttccttta
     1081 aaaacgttgt cgtggtagtt tcaaacaaaa ttcaaaattt catcacattc ctaggaaata
     1141 cgcaaacttt tctcacttat atagttaaat ctgttgagaa aataatcatt attatatttg
     1201 ttcaggatac tctggcagat cagcttttag tttttctact tgttcattga tttgtgtgat
     1261 acgtggttcg gcttggaatt taaaatcttc gatatctttt ttgattgctt cttggaaagc
     1321 aggaatcaca gattcagctg tttcttttgt ttcgatcaat gcgcttttaa aatgattcag
     1381 gctgttattt aaatcatttg tcagagttgt tgcttcttcc aattcttcga tcagtttctt
     1441 tcttgtttca ttcccgctcc tgggcgccaa taacagacct ccgacagtgc cagcagcagc
     1501 tccaaataac agtcctttga aaaagttttt aatcatcgca ggacacctgc tttacgattg
     1561 tttcttgaat agcttttagt tcttccgatc cataactatc ttgatgattg acaaagtgaa
     1621 tagaaaaatc atcctctttg ctgtagcgag ggactaaatg gatatgtgaa tgaaaaacag
     1681 attgataggc tagttcacgg ttgttattca agatattaag acctttcatt tctggaaaag
     1741 ctttttccaa tgcttgagca atcttaggca cacgcgaaaa aacctctcct gcaagttcgg
     1801 gatcatattc aaaaatatct gctacatgac gtttaggcac aactaatgtg tgtcctttcg
     1861 ttgcttgaga gatgtctaaa aaagcataga ccttgtcgtc ttcatatatt ttatagcttg
     1921 ggatttcttg attgatgatt ttgcaaaaaa tacagttttc catataaaac atcctttcta
     1981 taaagaataa atacttcagc ttaactttac cataagttaa agctattgta aggaaaaaaa
     2041 caaaataccg tcattttctt cgtggtataa taaataaaaa taaatggcgg aggaaaaaga
     2101 atgagtttaa ttatcgatca tgtcacaggg gggtacggac atctccctgt gttaaaagat
     2161 atcagtttcg atgtacagcc tggagagata gtagggttga ttggactgaa cggagcaggg
     2221 aaaagtacga ccatcaagca cattatcggt ctgctggaag cttcaaaagg aacgatagaa
     2281 atcaatcatc tatccttaaa gaaaaatacg gaagaatatc gcaaacaaat tgggtatatt
     2341 ccagagactc cttctttata tgaagaattg acattgagag aacatatcga agtcactgcg
     2401 atggcctacg atatacctat ggatatagca atgaaaagag cagaaaagtt gttggagact
     2461 tttcggttat ctaatcgtct tgactggttc ccagctaatt tttcaaaggg aatgaaacag
     2521 aaagtgatga tcttatgcgc ctttttgatc gagccttctt tatatatcat cgacgaacca
     2581 tttttaggct tggatccact agctatcaat gcattgcttg aattgatggt agaaatgaaa
     2641 aaagaaggag ccggtatttt gatgtctacg catattttag ctactgcaga aaaatattgt
     2701 gatcgttttg tggtcttaca taacggagag atacgagcag acggttctct tgagatgttg
     2761 agaaaagaat ttcagctgcc ggattcttct ttggacgaaa tctatattgc tttaacaaag
     2821 gagcaggcac atgagtagtt tttttcaaaa acgtttagca agacatcaaa agaaaatgat
     2881 gaaatatatg cgttatgtat tgaacgatca ttttgtttta gtttgtcttt tcttgattgg
     2941 cggtgtggga ttatattatt ctaattggtt gaaaaccctg cagccaccgt ttgcaattgg
     3001 agggctcata atctctgtat tttggatgct ctgtttattc gtgggtaaga tggctacttt
     3061 tgcagaggca gcagatattg tttttttact tcctaaagaa aaagaaatgc aaacctattt
     3121 ggaacgagct tttcgctatt cttgcatttt tccgttgatc tttttgatat tggcatgcgg
     3181 attggccatg ccattagtgg tggttagtac ggagcaatcc ttttcgatgt ttttcgtcta
     3241 tctgatcata ttatggagtt tgaaattcag tcatctgcaa atcaagagag cagaattgtt
     3301 tcgactagat caacaagtta ttcgttattg gaaattaggc tggttttttt cttctctgat
     3361 ctttttgttt ctatcatttt attggctaat atgggcagga atagcaggtg cattagtaca
     3421 ggcaagtcta tattactggt ttttgtggaa gcaacaagaa aaaggattgg attgggagaa
     3481 aatggtgcat gtagaagaac ataggttgca tcagatctat caatttatca atttattcac
     3541 agatgttcct gaaattacgg caaagatcaa aagaagacgc tatttggatc ctatattgac
     3601 tcatattaaa agagaatcaa aaaacactta tctttacttg tttgcaagaa gattggcaag
     3661 aggttctgat tttagtggtc tttatttccg attagtaatt ataggtgggt tattgattgc
     3721 cggagtagtc gacttttatt ttagtctgat tatcggtttc ttatttttat atttgatcgg
     3781 tttccaactg ttacctttag cgaaccagtt ccgctatatg gttctcgttc agctttatcc
     3841 aatccataag aatcagaaga agcaggcgat tcagtatcta ctcacatggg tattaattat
     3901 cgcgtcggtt atttttggtt tgattgctgc agtcgtatta gatgggcagc agcgatgggt
     3961 tccgctagcg gcttatttga ttgttactat gctctttgtt aaactatatg taccaactcg
     4021 tttgaagaag atgtcagact gaaaacaaag ctgtgtaggg taaaaatgag aatgaaaaat
     4081 agagcgttga cagtaagaat gctgttcgtt ctattttttt tgagaaaagc tggttttttg
     4141 aatggcaatt aaatgaaaaa gaacgtatat gtgcttgacg tgtagtgttc aggcgactag
     4201 aataataagt actgcaaaac aaagagtaag aggacgggag aatgcatgat ggagtttcag
     4261 ttagaccaag agtggcggtt acgtcccatc aaaggcgaca caggaaaaac atacataggc
     4321 atgcgtgatg aggacaaagt ctttattaaa cgaaacacga ctccgatgtt ggctgctctt
     4381 tcaagggaag gaattacacc aaaacttgtt tggacaaaac gaacggggaa tggcgatact
     4441 ttgacagcac aagagtggct ggaaggtcgc ttgctgactc ctaatgagat tggtcaaaga
     4501 aacgacgtga ttgatgtttt gtaccattta catcattcaa attctcttaa aaccatgctg
     4561 acccgtatcg gtggtcgtgt catgacgccg gagaagatgc tgcaaaaata tgaaaaagaa
     4621 ttaccggaag aactccagaa aaatcaattt ctgcaaattg tccatcgttt tcttcgtgag
     4681 cagatcccgt caatagataa ggaaaactta tcagtagtac acggagatgt aaaccatcgt
     4741 aattggttag tatgtaaaaa ttatttatat ctagtcgatt gggattcaat catgtttgca
     4801 gaccctgcgg tggatattgc aacgatttta ggaaattatg tgccactgtc tagctggaat
     4861 cagtggctga tcagttatgg catacgcccg actgacgaaa tgcttgaaaa atttcattgg
     4921 tatgcggcca tgaatttgct ccaagaaatc accagatatt gcaaacgtga agatcatcga
     4981 cggatgaacg aagaaatttt acaattaaaa cgaattttca gtggctgagg tttggcccaa
     5041 actctgcgga gatttggcca gaccttttta attagaggaa aggaatggag ataaataatg
     5101 cgtgtccgaa atcgtccggg agcagcggaa atgttagcgg ctcacccgaa ttttgtaatt
     5161 agtgatccaa cattgtggaa gggaaaatgg aatgaattgt ttgaaaacga tcatccaatc
     5221 cacattgaga ttggtatggg gaaaggccag ttcatcactg gaatggcaaa agcccatcct
     5281 gaaatcaatt atattggagt agaaatgcag gtcagtgttg tttcgattgc actagacaag
     5341 ttgatcgaac aaccgcttcc taacctgaaa cttctacatg tggatggatc agcgttgaca
     5401 gagtatttcg ctgacagcga agtagatcaa atctatctga atttttccga tccatggcca
     5461 aaaaaacgtc atgaaaaacg gcgattgaca tataagactt ttcttgctgt agatgaacag
     5521 attcttcgtc caaatggaga aatccatttc aaaacagata atcaaggatt gtttgagtat
     5581 tctctggcaa gtttttctca atatggcatg atcctcaaac aggtatggtt agatttacat
     5641 caaagtcaat ttgaaggcaa tattatgacg gagtatgaag aaaaattttc ttcaaaaggt
     5701 caacgtattt atcgagtaga agctcaattt caagataaga ccaaaaagaa aagtctgtga
     5761 aactaacttt ctgctttcat aaaaacatta atatccataa ataaatccca gaaaatgaac
     5821 attacagatg taatgtattt tctgggattt atttgattta aaatgtattt atttcatcgg
     5881 tttttccaca aggtggaatc aattggaagc gagaaaatga aaataatcct ttgtcggtga
     5941 tcttcaattc gggtatgaca gataatgtta agaaagaaag tgtcaaaaaa ggatcaaagg
     6001 ttttagctgc tcctagttga tgtgctttag ttattaattc attcaactgt tcatagacga
     6061 tcgaaaaagg ttcctgtgtc atgattccgc ctataggaag agaaacacaa gctatttcct
     6121 gctgattgtt gacaacggtc atcccgccac ctttttgaat caaaagatta gcagcataca
     6181 gcatatcctc gtcgtttgtc ccaacgacaa ctagattatg cgagtcatgg gcaatcgttg
     6241 tcgctatcgc tccttgtttc agctgaaatc ctttcactat gcctaatcca atgtttcccg
     6301 tttgatggtg acgctcgatc accgcaatct ttaattggtc tttttcgatt gaaggaatga
     6361 atcgttcgcc ttgtcggtcc actttttcta ccaaatcttt agtcactaga ctattcggga
     6421 taatttcgat cacatgggct tgtgaagaag aaagaagcag ctctaacgaa tgtggattta
     6481 atggttgagc gtttagtttt ggcagacaac acgtatatga atttgaatga tcagcaaata
     6541 aatggtcttg gagttgccca gattgaacaa ctaatgttcc cttatagaaa acttcatgaa
     6601 ttggcagctt ggtaagatcg tctgttaaga agaaatcagc ttgataccca ggggcaatcg
     6661 cacctagtgt tttcaaatca aaacattctg ctgcattcaa tgttgccatc tgaatggctt
     6721 gtaatggatc gattccaagt gcaatagctt gacggacgat atgatcgatt gatccttctt
     6781 ttactaaatc attgatcagt ttatcatcgg tgacaaagaa gcatcttctt gaattttcag
     6841 gtgtgatcgc cggtagtagc gatgccagat cttttgcaac ggtcccttct cgaaccatta
     6901 aatacatacc aagatccaaa cgttctttag cttcttttgc agttgtagct tcatgatccg
     6961 tccgaatacc tgctgcagga taaacattta actcttccat gccgatacca gctgcatgcc
     7021 cgtcaatttt tttcttatgc tggtgcatca attggatctt ttctaggatg tctgtttcat
     7081 tatttgctac tgcttgatag ttcatgactt cagctaagcc taaaacttcg ggtttttgta
     7141 aaaaaggggc taattttttt gcatttaaat ttgcaccatt ggtctcaaaa ggggttacag
     7201 gtacacaaga aggtagcata aaaaagatat tcatgagtgt ttgtcttgca tcttctatca
     7261 taaactcaag acctgtactc ccagcaatgt ttcctatttc atggggatct gtcactacag
     7321 tagtaactcc atgaagcaag gatacttttg aaaattcgct tggtgataaa agtgagcttt
     7381 cgatatgcac atggccatcg atgaatcctg gcactagata ttgcccatta gcattatgta
     7441 cgctttttcc ttcgtattca ccgattccta cgatcattcc ttcgcagatt gctacatctt
     7501 tttccatgat ttctttcgta aagacgttga cgatcttagc atgcttgatc actaggtcag
     7561 caggcttttt tttacttgct acttcgatat atttccttaa tgttgtttcc aatggagtgc
     7621 ctcccaagat aaatttttat aagtataaca taaattcggt ttttgccaat atttttttgt
     7681 aaattcattt tatttgaaac aaaaagatca aaaagacgta tgttttttgt ggaggggaga
     7741 ataatggaaa taaaacgatt atcggatcaa atgataagtg aaagttttgc caaacatgcc
     7801 caagacgtat atatattgcc agcttcagaa gtattttaca tctattttcg ctatggaaaa
     7861 aaacgaacat tctggaagaa agtctcttcg gcagatggac agcaactgat ctcaagattt
     7921 aagtatttgg gacagatgga tatcggagaa aaaagaaaag ctcagttagg tgcatttact
     7981 tattctttag aaaaacagga agttcgtctg cggttgtctt ctgtaggaga ttaccttcaa
     8041 cgagagagtt tagtgattcg gctattatac ggtacttttg aaacacccca tcgttgcttt
     8101 agttctcgag attttgcaac tattttacag aaaaccgtca gacgtggatt ataccttttc
     8161 agcggaccag tcggatcagg caagacttct ctaatgtatc ggttggcttt ggaagaaaaa
     8221 ttgcaagtga ttactatcga agatcctgta gagatagaag aaccgttttt tctgcagcta
     8281 cagatcaatc cgaaaatagc acagacttat gatacacttt tgagactcgc attacggcat
     8341 cgtccagact tgctaatcat tggcgagata agagatgaat caactgcaca tgctgcggta
     8401 agagcagcac taacagggca ccgggttttt gcaaccgtac atgcacgcaa tcttgaaggt
     8461 acattggtca gaatccatga actgacaaag aaaaaagaag agctatctga atgtttagct
     8521 ggtgtcattt atcaagaact tttggaagat gccaatcacc atccttctgt tttatggagt
     8581 tatcaattta tggaggaaga atcagtaaaa aaatcatggg aggaaagtta tgaagaagct
     8641 caggaaaata tttggcagtc agaactattc taaaggatta acaagaagcc agcaaaacca
     8701 gtttgcacaa accatgggag aactgctttt atctggtttt agtctgcaag aatcccttta
     8761 tttattactc acgatccgtt cgtttccacc tgttattttg gcaaatatcc aacatcagct
     8821 aaaggaaggg caagcgttta gtgaggtact ttcgcaaata ggatatggag cagagcaact
     8881 ggtacagatc gagttagctg aacgtcatgg gaatctgccg acaactttgt tggaaattgc
     8941 caaacagttt cggcttgttg aaggatttcg caatgatttg aaaaagctgc ttgcatatcc
     9001 gatgctattg ctcacctttt tgctaggtat cttgtttagt atgcggttgt tcgttttgcc
     9061 tcagcttatt ggttcaggaa tggtcatgca agagcattgg ggtattcagc tgatacaagt
     9121 ctttcattgg tatgttctac tattcatcct agcggttact ctattggctt tactagtaag
     9181 gtatcgtctg atgaagttgt ctcctgttga tcagagtgaa tggataagca gacaattatt
     9241 gattggaccg atatattcct tgtatcaatc ggcttatttg tcactagaga ttggaaagct
     9301 ttttcatgaa ggtttcgaat taaggcaaat catagtctgc ttaaaggaaa cgaaacaaga
     9361 aagtctgatt cagcatcttg cctctaagtt gattcaagga ttggaggcag gtatgtcatt
     9421 ggctgagcag ttccagaatt atcgttttct atcagaagaa ttcagtaaaa ttgttttaca
     9481 gggagaggca aagggaagtt taggtaaaga actgattttt tacagcgaat ggacaagaaa
     9541 acagcttttc aaacgtcttc atcactggat gcattttatt cagccaatca tttttttggg
     9601 cgtagcggct ttgattttat tagtttatgc cgctgtttta ttacctgttt acggaagcat
     9661 agaggaggtt ttgccatgaa aaagagaaaa atatcttatt atagcggttt tacattgatc
     9721 gaaatgctaa tcgtcttact gatcatcagt gtattagtgt tgttgttcgt ccctaatttg
     9781 tcgcgttata ggaatcatgt agaccaagaa agtcgtgaag cgatcatcca gttggtagac
     9841 acacaaaaag agctctatgc gcttcaaaat aatggaagag ttcctactgt ggaggaattg
     9901 ttgaatgaag gctatatcaa acgagaacat gcagaaatct atcagcggcc ttgaaggtaa
     9961 ggttttagca tcaggatata cgttgtttga atcactctta ctgattttat tacttacaac
    10021 gatcctttct tttccagttc tcgcattttc tgcatggaag aatgaattgg cagtctatca
    10081 atttttcggc caatttgaaa aacgaatcta ttctacgcaa aaaatcgcta ttgtgaatca
    10141 gatccaaaca ggcttttatt ggaatagtga agagaatcaa atcatttttc atgttccgaa
    10201 tccagataaa ataaattggg aagtattaga tattcctgat gagattacct tgaaacgcca
    10261 tgcttctatt acttttgctg ctagaaccgg gaatgaaagt tcactaaaag cttatcaatt
    10321 ttattgggaa gaaaaagagc ggacgattac gtatcaattc cagataggga gtggtcgcta
    10381 tacaaaaaga attgaataaa acaggatttg ttttgttgga gtcccttaca gcgcttatga
    10441 tcgcttctgt ctgtgtctac ttagtgagct ttggccagct gtttttgctt caggcagaaa
    10501 agaaaagtgg ggaggaactg ctatttattc gaatgattta tgaagatatc aagacgcagc
    10561 ggatgtatca caaaagacca gagcgaaggg aagaaatgat cccatacgat atccaaattg
    10621 tcgataataa tggagtaatc gaaaaagtgt ttgtcaatat ggagaaagaa gcgatcagta
    10681 ttgaaaaaac aagtaattcc agcatttacg ctgattgaat gtctaattgg actgatgata
    10741 ttgagcagtc tattgctcac attcaatctt ttgattcagc aaaccatcca agtaagtgaa
    10801 tttttgcagc gtaaagatca aaaagagtgg ctaatatttt tagcacaatt agaagaagaa
    10861 ctcaagagca gtcacaatgt ctctgtgaaa aatcagcaac tgcactacac gatcgaggat
    10921 aaacagtata tcatcgaaag atatcaggca atgatacgta aaagaagaac ttccggcgga
    10981 catcaaccta tgttaacaag tatcactgag cttcaattat tggaaaaaga acacacaatt
    11041 tctttttatg tacactttga aaatggagaa gatggatatg ccatatggac aaaaaacgat
    11101 cagtagaaaa tgttataagg gctctatttt attttctgtc ttgcttttat ttcttttatg
    11161 tacgttttta ttgatggcag cattagaaag ctaccgtttg tcaatggatc ttaatatacg
    11221 aatcaaaaat tattatatag caaaaataat gaagcagatg gctttatcgg aacagtctac
    11281 gcttgccgaa agttcggaag gagtttttta ctacacaact ggatctgtaa catatcagtg
    11341 ggtacaacag tccctattct tagaggtgga agtctcaccg tttatctttc ggttcacaga
    11401 agaagtaaaa aatgatacag atactagtac agaatgatgg aatgttcgtg aaaacgccct
    11461 cttttctgtt ataacactgt ttttaataaa aaaatgtttg ccgtttcaca aataaaaaac
    11521 agtgaaaacg attgaaattt tgtcaattgt tcgatagaat taaacaggtg aaaaaattgt
    11581 tataatatat ctagaaatga ggtgcaccat gttaccagaa aaaatggaac aagggtttaa
    11641 tcaaaacctg gaagctattc agcttttaca gaatgcattg ggaacttcct ttttagaagc
    11701 ctatgtcgaa aatgcagaaa atttaataga tgattatcaa gtacgtgtgg tggatggagt
    11761 gccgacaaaa gagacaacgc aacggatcac agctctttat gaagagttga agaaactttc
    11821 tttcgagcca gaagaatggc gcagactgtc tcaattactt ttattgaagg gcagccagac
    11881 agaacatttg caggcgaacc atcaactcac tcctgatagt atcggttttt tatttgtttt
    11941 tcttattgaa caactataca caaataaaaa agcaccggtt aagattctgg atattgcggc
    12001 aggaatgggc aacttgttat taacagttct tttaaaccta tcgaatgcgg ggtaccaaac
    12061 agaaggaatt ggtgtggata ttgatgatac attgttggct gtcgcagctt ctacaagtga
    12121 tttgacacaa gcaaatgttc aatactttca tcaagacggg ttgcaggaat tattaataga
    12181 tcctgtcgat tttgcaatca gcgatttgcc aatcggttat tatccgaatg acgaaaaagc
    12241 aaaagagttt ttaacaagta ccgaagaagg ccatagttat gcacatcatt tattgctaga
    12301 gcaatcaatg aaatatgtga agccggatgg ttttggtttg tttttgatgc cgagtggatt
    12361 tttagaaacg gatcaaagtg aagaaatcaa aaaatggttt aaagaagaag gttatcttca
    12421 aggaatgatt caattgccag atgaattgtt ccgtaataaa caatcgcaaa aaagtatttt
    12481 gatcttacag aaaaaaggac cacaagcaca acaagtaaaa gaagtcttgc ttgtgaaact
    12541 tgcttcatta aaagaaccag agaaggtgac ggaatttttc aacgagttta aaaactggaa
    12601 atcatctgct ctgtaatcat tcaacgagaa ggagagtcat atgtctaaaa caattgcaat
    12661 caatgctggg agttcaagtt tgaaatggca attatatcaa atgccagaag aaaccgtagt
    12721 tgcgaaaggg atcgtcgaac gtatcggttt acaagattct atttttacca tcaagtacgg
    12781 cgataatcaa aaattcgaac aaatcatgga tatcgaaaac cacgaagtag cagtaaaaat
    12841 gctgttagat cagttgattg aactgcaaat tttaagctct tatgatgaaa tcaccggagt
    12901 agggcaccgt gttgttcacg gaggagaaac ttatggggat tcagtagtga tcgatgagaa
    12961 agtaatggac cgcattgctg aattagcaga atttgctcct ttgcataatc ctgcaaactt
    13021 aatggggatc aaagcgttta aaaagatttt acctgacatt ttgagtgtgg ctgtattcga
    13081 tacatctttc cactcaacaa tgccaaagca aaattattta tatagcttac cggtgtctta
    13141 ttatgaagat ttcaaagtaa gaaaatatgg cttccatggc acaagtcatc gttatgtgtc
    13201 ggaacgtgcg gcaaaattat tgaacaaacc gatcgaagaa ctaaaaatca tcacttgcca
    13261 cttaggaaat ggtgtatcga tcacagcagt cgatggaggt aaatccatgg atacttctat
    13321 gggattcaca ccattagctg gggtgacaat gggtacacgt tcaggtgata tcgatccagc
    13381 tgttctccca tatttaatgg aaaaattaga gattgatatt gatgaaatga tcaatgtttt
    13441 gaacaagaaa tctggtctgc taggcctaac aggattgtct agtgatatgc gtgatcttga
    13501 aaagaactat gaaaaagaac atattcgttt agcttatgac attttcgtgg accgcatccg
    13561 aaaatacatc ggcggttatg tgacaacgat gaatggtgtt gatgcaattg tctttactgc
    13621 tggtatcggc gagaatgacg gacatgtacg tagtgaaatc atcaaaggta tgtcttggtt
    13681 tggttgtgaa atcgatccag cgttgaatga attgcgtggt gaagaagtag atatctctac
    13741 aaaagaatca aaagtaagag tattagtgat cccaacagat gaggaactaa tgattgcccg
    13801 tgatgtggag cgtctgcgcg gctaagccaa agcattcgtt aggataaaag atgaaacgca
    13861 tgcaagaggg aattcttgca tgtgtttttt ttgcggagaa gaccgcaaaa tggtaattta
    13921 acttcaattt cccttctttt ttacaggatc ttcttaggga attttgcttg gaaaagtggt
    13981 caaaagggaa aagactggca ttttatttat tgattttcag gaaaatccca atattttatt
    14041 ttcattcatc tttataaggg acattacata tgtaaaaaga aaaaacattt ttagatcagc
    14101 ctcgtgggct gtcgacttta ttcttcgctt tatgtttagc tagaaaaccg atccgaaaca
    14161 ttcattgatc gaataataaa aacttgattt tccacctgta ctttgcctga atcgtgaaag
    14221 cacaggtatg aaaatcaagt tttttgttgc gaaaaaacgt cctagtcaat cagttggccg
    14281 agcggctggc ggctgatctg ctcctgatca ataacgagtg cttcttggaa gtgttgctgc
    14341 aaaaagtagc ggtcatggct gactgctagg agcgttcctt gatagtcttg cagcatttct
    14401 tcaatttctt ctcgagcata gctgtcgagg tgattggttg gttcatccaa aatcaggacg
    14461 ttgatttttt ctaaaaagag tttcagcaac gccaaacgga ttttttcacc accggataaa
    14521 tctttgatcc gtttacccac atcgctttga taaaagccac ttttggctaa ttgccgtcga
    14581 gcgacttctt cgtttccagt caatgtgatg gcgtaagcca gcaaacggct atcgggatcg
    14641 gcaaactgca gctgctgcgg caaataaccg atttgcaagc tactacctaa tttgatggtg
    14701 ccaccgtaag gcttttcctc accgagtaat aatttgagta aggttgattt tcctgcacca
    14761 ttgtcgccga tgatggctaa atgatctcca cgatagactg caaaactgct gtctttgaat
    14821 agtacttgat cagcgtactg ctggtgcaag ttttgaatga tgaaaacttc tttgccagat
    14881 tttccaccat tagacaagct ttgcagccga tttttcgggg gaggtggcgg tttgacgagt
    14941 gtcagtttcg ccaggcggcg ttccagttct ttggcttttt tgaagaagga ctcattgtct
    15001 ccttcatgtg cccactgacg aaaacgacga atcatgactt tcaaccgctg gacttctttt
    15061 tgctgcagtt cgtaattttt tgtcagctca gccaagcgag cttctttcaa gactcgaaaa
    15121 cggctgtagt ttccgggata ctcaatcaat gcaccttctt ccagttccca tgtttttgtg
    15181 gtgacttgat cgagaaattg ccgatcatga gaaacgacca gaaccgattt ttttgtattc
    15241 tgtaaatagt tttccaacca gcgaatactg ttaagatcta aatgattggt cggttcatcg
    15301 agtaaaagta aatcggcatc ggctgctaaa attttggcta aagcgacttt aactcgttcg
    15361 cctccgctaa gttctgttaa ccgtgcttcc tgacggtgtg gaatccctaa gccttgcaag
    15421 atcgctgcaa tccggtcttc taatacgtaa ccgcctagtt cttcaaagcg atgctgcagc
    15481 tcaccgtaac gggccagaca cttctctaag tttgtatctg gttctgtcat caatgcttcg
    15541 tagcgactca tttgctgttt gaggtcctgt atctcagcgg cagaatggag caagtagtgg
    15601 ttgacggtcg cttcggaaac tgtaagttct tgttcgaccg tcccgatctt taagcctttt
    15661 ttgcgactga taacaccgct gtcgacacct tctctgccgg tcaagatttg taaaaaggtt
    15721 gatttgccag ttccgttgat accgactaag acaatttttt caccagcttc aatttgacag
    15781 gataaatcct caaataatgg attcccgccg attgtttttt taatagaaat tgcttgtatg
    15841 atcatgatct ctctccttga gaccactcaa aaaggccatg gttatccatg gcctgtgtta
    15901 tttaaactca gattatcatg agaaaaatac cttgagatgg ccattgattt tacgtgaatt
    15961 caactgtttt ggacaaactt gtcccgtcaa cagcaactgt aaaaccaatt gcatggctaa
    16021 atgcgatatg gtatcgttgg taattttcat tacttgttat tttagtcatg cgcatcatag
    16081 tattttccct ccttcgtgta gttgtctttt ccagtgtacc tgaaagtgtc agaaagttca
    16141 actagcgctg acatttactt aatatttatc gatgtgtaat tctgtcagag agcttttttt
    16201 atctgactga gggtaatgtc tcgtggacca catcattgta ttctgagcta aaccagcgtt
    16261 caggcgaaat catatggtgt ttgataaaaa actttgtcta catgacttcc ttttgccatt
    16321 tgcttaatac cgttgcatta aacagctctt ctctttggaa atctcaccaa catcgcgttc
    16381 aggagtatcg ttgtggttgc aatggcaagc ggtagccttt catcaattct tgaatgattt
    16441 accgaatgta gcacttgcct tctcgcggta cccgaacccg aaataaaatt ttagtttggc
    16501 gataaaatcg gtatccgcca tcgatacttc atattttttc gtatcagatt catcggaaac
    16561 ttccacattg acgaaccaat aagctttggc atgcttaggt cgttttaaac cacaccaggg
    16621 cgactaatcg ccctggtgtt cacttttttt tattttactt gaactcattg actgttttcc
    16681 ctttatttta aacattataa agaccgatga gtgcttgccg cgtcttttgt ttttatcacg
    16741 gttttttgtg caaaaaaacc tgccgatagt tggcaggtcg tctacgtgtt aagctttggt
    16801 gtggtcttta gctggcaatt ggttttctaa atcagttcgg acaacaagca catcgcaagc
    16861 tgcgtttcta ataacatatt cagaaacaga accaataaat aaacgttcta cagcattcaa
    16921 tcctgtggct cctaacataa tcaaatctac ttgattatct tctgggattt ctctggcaat
    16981 aatttgtttt ggtgagccat attctacgac agaggttaca ttattcaagc cagctttctt
    17041 cgcattgctt tcataatctg ccaatgtttg ctttgccatt tcagtagctt gttctgcaag
    17101 cattccatca aaggatgaaa cggtttggaa agaacgtgtg tcaatgacat gtgctaatag
    17161 aagttctccg ttatttcgga tagctacatt gaccgccttt ttaaaagcta attcagcttc
    17221 atcagaacca tctacggcaa ccatgatttt tttgtattgt tgtaacataa gcattcactc
    17281 ctcaagtcaa tcgtattgta ctcttttatt gtagaacact ttagggaaaa agagaattat
    17341 ttactgcttt ggttcagcaa ttttttttgc aaatcggata ctgaaggtgg cagaaacaat
    17401 aattacgatc agtaaaaaga ccaaggcaat aaagcgttga ttgaaaaaag ctgttaaaat
    17461 acccattacg cctaaaacag catatgcagc tccgtaaaat tgtaggaatc cttgattttt
    17521 atcatttttg ttgatcaata caaaaaagat atcctgttta ttaaataaat aatagctaac
    17581 gaataataat actaaagtgg cgataatcat taaaatccta atcatgaaca aatcctccaa
    17641 aataagcaac aaatagaaaa agcaatttgc caagagcaaa ttgctttatt ctttaaaaga
    17701 tggtaatctc cgttaatgcc gttgtttgtc ctgtagtatt gatcccgcga attcaagcag
    17761 gtgggtccca tggtcgtagc ttcgaactac caaatgataa ggcatgttac cagcagtgac
    17821 acaagacggg tcccaagata tcaataaaaa tgttcggtca acacacaaag aggacaattc
    17881 gaacatcaca gatttcccac ctctatagta gcatatgaat ttaaaaagag caatgagaga
    17941 gctcgcagaa acagaaccgt tttcttgtct agttttttgg aggatgcttt ttccactctt
    18001 ttatacggta atactgttga cccagagcct gttcatattt ccctgtatct tttggctgat
    18061 aatactgagc attcttcagt ttatctggta aatattgctg attgacccaa gcctgatcaa
    18121 aatgatgagg atactgataa cccacgccac ggttgagtga tttcgcacct ttgtaatggc
    18181 tatctctcaa atgatcagga acatctccag ctttgccctc tcgaatatca gcaagtgctg
    18241 catctaatgc catgtaggca gaattggatt ttggcgacaa gcaaagatcg acgacgacgt
    18301 ctgctagtgg gatacgtgcc tcaggcaatc ccaatttttc agcagctaat acagcgttca
    18361 ctgtacgagc cgcagcagca ggattaccta aacctatatc ttcataaccg ataaccatca
    18421 gacgccggca aatacttgcc agatcacctg cctcaactaa acgggcgaga taatgaagtg
    18481 cagcatccac atcacttcca cgaatcgatt tttgaaaagc cgaaataacg tcataatgag
    18541 catctccatt tttgtcatgg gtaagagctt ttctttggac acattcttca atgatcgaca
    18601 gtgtaagatg aatccttcct tctttatctg aaggagtcga tagtgtagcc agttccagcc
    18661 cattcaatgc actccgcaaa tcgccgttcg ttgcacgcga aaggtgaaga agagcatctt
    18721 cgtctaactg gattgcttgt tgtccaaggc cgcgttcttt atcccttaaa gcatgttcga
    18781 ctgccaactg gatgtcttgt tctgttaatg gtttgacttc aaagatctgt gtacggctgc
    18841 ggatcgctgg attgatggta atataaggat tctcagtagt cgctccgatc aagatgattc
    18901 ggccactttc taaatgagga agcagaaaat cctgttttgt tttatctaat cggtgtactt
    18961 catccaataa gaggatcact gtaccactca tttttgcttc ttccgctaca acttgtagat
    19021 ccttttttga atctgtagcg gcgtttagca tgcgaaatgc ataattagta gagccggcga
    19081 tcgcacttgc gatactcgtt tttccagtac caggaggtcc atatagaatc atggaagaga
    19141 gcatgcgagc atcgaccatg cggcgtatga tttttccttc cccgactaga tgctgctgac
    19201 caacgacttc atcgatcgtt cgaggtctca tgcgataagc caatggctgt tgcataggat
    19261 tttcctcctt cttgttttta cgtcccttaa ttatatcata tttagcaaga tgcctagaaa
    19321 gaacagcctt ctgttgtttt gttttcatag cttatccaac taatattgtg tatgataaag
    19381 taggtgagaa aaatgacgga taaacaaaag atagatgaat ttttttggaa taaaacgacg
    19441 ccagaagtgg cacgggattt attggggatg tatttggagc atgacacacc agaaggaaga
    19501 ttagctggat atattgtaga cgcggaagca tacctaggtc cagaagacga ggccgcccat
    19561 agctatggtt taagaagaac accgcgtgtg cgggcaatgt atgaaaaacc aggtacgatc
    19621 tacctttaca cgatgcatac tcatcggatt ttgaacatca ttactcagcc agaagggatt
    19681 cctcaaggag taatgatacg tgcaatcgaa cccgccgcaa tgattgatca aatgagtaaa
    19741 aatcgtggcg gaaaaactgg tccagatatc agcaacggtc cgggaaaatt agtggaagca
    19801 ttagcaattc ctcaagaact ttatggtcag tctatagcag atagttcgtt acgcttagtt
    19861 tttgagaaaa aaaagacacc aaaaaagatc aatgctttgc ctagaatcgg tatcccaaac
    19921 aaaggtgtat ggacagaaaa gccgttgcgt tttgttgtgt cgggaaatcc ttatatctct
    19981 ttacaaagaa aaaaccaaat cgagaaagat tggggttgga ggaaagaaaa tgaaaaagaa
    20041 ggatctatta acatatttag atgaaataat tgaaaaacaa gtgacagact atgatgtagc
    20101 tttagattgg gatacaaaaa atcatacgat cgagatcgtt gttcgcttgt ttgcagaaaa
    20161 taaagcacaa tttgagattg atgatgcaga agggatcgtt tcgcaagaac caattatcga
    20221 atttgaagac ggcgtacttc tttttaatcc tcagaaatct gtttttgatg aacgcaatta
    20281 tctagcagta atcccttatg aaggaaaaaa agggttggca aaatcagtag ctgattcttt
    20341 agtggaatat ctaaatgaag tattagctca agggcaaagc gatttgcttg attttttgga
    20401 tgaagacgat gacgaagctg tattcgagct tcactgggat aatcagcaac tagcagaatt
    20461 agtagaagta aaacagcaaa atgaagcaga tacttattta ccgtacccaa gttattaagg
    20521 agtgacagat atgaaatgga atgaagtaaa agtagaaact gaaagtgaag cagtcgaagc
    20581 agttgctaat attttgatgg aggcaggggc gagtggtgtc gcgattgaag attctttaga
    20641 tgttgaacat tttcagtcgg atcaatatgg tgagatttta gataaaaatg attttacatc
    20701 gctgaaagaa ggcgcactag tcatggctta ttttccggaa acgactttct taccggaaat
    20761 cttgcctttt atcaaagatc gtatcacaca attacctgag tttggtctag ctattggaaa
    20821 aaataaagta acagttagtg aagtagagga aagcaactgg gcaacagcct ggaagaaata
    20881 ctatcatccg gtgcgtatta cgcgaatgct gacggttgcg ccaagctggg aaaactacca
    20941 gacagaagat cctctagaaa aaattatcac actagatcct ggtatggcat ttggcacagg
    21001 cactcatccc acgacttcct tgacattgac agcactagaa acagtcttgc gtggtggtga
    21061 aacattgcta gatgtgggaa ctgggtcggg cgtgctaagt attgcagcaa aatatcttgg
    21121 tgcaaaagat gtctatgcct ttgatttaga cgaagtagcc gttcgttcgg cgaaagaaaa
    21181 tatggatatg aatgaaatag ccaaagatgt ccatgtatca gctaacgatc ttttgacagg
    21241 aatcgagatt gaaagtgatg taatcgttgc gaacatttta gcagatatca tcttattgat
    21301 gatcccagat gcttggcgtt tactgaagca aacaggcacg ctgatcgttt caggaatcat
    21361 tgaagagaag aagcaaatgg ttctggatgc catgttagaa caagggttta tcgtggatca
    21421 aattttccag caaaaagact ggtatgcaat tattttgaaa aaaccagagg tggaatagat
    21481 gcagcggtat tttttaaaag aatcatatga atcaaaagac tattttgttt tagaagggga
    21541 aaactatcac cacattgtac gggttatgag gatggaaccc gaggatgaag tttttttggc
    21601 attcaatgat caaatagcca ttgttgcaga aatcacggag attactgaag aggctgttta
    21661 tctgaaagaa attaaaaaag aagaaaatca aaaggaactg ccaagttcga ttaccatagc
    21721 gagtggctat cctaaagggg aaaagctgga ttggatcgtt cagaaatcca ctgaattagg
    21781 agcgcaagct tttcttggtt tcccggcatc agcctctgtc gttaaatggg atgcaaaaaa
    21841 acgtatgaaa cgacaacaac ggcttgaaaa gattgctcaa gaggcagcgg aacaatccca
    21901 tcggcagatc gtgccatcag tagagttatt ggaaacgaaa caagatttat ttgagcggct
    21961 agatgattat gacgtggtct tggttgctta tgaggaatca gcaaagcaag gcgaaaaatc
    22021 aaatgcagcc aaaattctta gtgaaacaaa accagatagt cgaatcttag ctgtctttgg
    22081 accagaaggt ggattatccc gcgatgaagt cgattatttt gtagaacaca aagggatact
    22141 ttgtggatta ggaccaagaa tccttcgtac agaaacagca cctctttatt tattaagtgc
    22201 tgctagctat caatgggaat taatgaatcc ttctttcagc taacgttctt cattaataaa
    22261 agagtatcga cagaaagaat tgaaaaaata gtcaacaatt tgtataatag aaacaattat
    22321 ttatacaatg gatacgaaaa atcgtttgaa aaagctttta aaaagcgaac atttccgtaa
    22381 aaaaacgtat aactgcacgg attttgggac aattatcaaa cagatatttg tcccgattcc
    22441 cgttttaccg aaaaaagaaa gggggatcga ttcctgtgac aaaagaagaa attttaacgg
    22501 gacccggtgt cataaagctc gttagtcaat acatgggtcc cgaacatgta gcatttgttg
    22561 agaaagcatg cgaatacgcg actgctgcac atgacggaca gtttagaaaa tcaggcgaac
    22621 cctacattat tcatcctatc caagtcgcag gtatattagc agatttaaaa atggatcccc
    22681 atacagtggc tacaggcttc ttacatgatg ttgttgaaga tacagaaatc actttagaag
    22741 atctgagaga agaatttggc gatgacgttg ctatgttagt ggacggcgta accaaattag
    22801 ggaaaataaa atataaatcc cacgaagagc agctagcaga aaaccaccga aagatgctgc
    22861 tagcaatggc tcaagattta cgagttatca tggtcaaatt agctgataga ctacataaca
    22921 tgcgtacgtt gaagcacctg cgagaagata agcaaagaag gatcgctcag gaaactttag
    22981 aaatctatgc gcctcttgca catcgtctag ggatcagccg gatcaaatgg gaattagaag
    23041 atacagcact tcgttatcta aatccaaaac agtattaccg tatcgtccac ttgatgcaga
    23101 cgaagagaga agaacgtgaa aaatacgtaa gtggtactgt tgaagatatt cgaatagcga
    23161 cagaagagct ggggattttt gcagaaatct atggacggcc aaaacacatt tattcaatct
    23221 atcgtaaaat gaaagatcag aaaaagcagt tcaacgaaat ttatgacttg ctagctattc
    23281 gagtgatcgt agattcaatc aaggattgtt atgctgtatt aggagcaatc cacacaaaat
    23341 ggaaaccaat gcccggcaga tttaaggatt atattgccat gccaaaagct aatatgtacc
    23401 aatctttgca tactaccgtg atcggtccgg caggaaatcc ggtagaaatt caaatccgaa
    23461 cacaggaaat gcatgaaatc gctgaattcg gggttgctgc acactgggcc tataaagaag
    23521 gaaaaaatga aaaagtagaa ccagatggta tgacgaaaca attaagctgg ttccatgaga
    23581 tactcgaact tcaagacgaa agctatgatg cttctgaatt tatggaaggc gtaaaaggag
    23641 atatctttag tgataaagtc tacgtcttca caccaaaagg agacgttact gagttaccaa
    23701 aaggatccgg accattggac tttgcataca gtatccatac agatatcggt aacaaaacca
    23761 ctggtgcaaa agtaaatggc aaaatggtgc agcttgatta caaattgaaa aacggagata
    23821 tcattgagat catgacttct ccaaattcat ttggcccaag tcgcgactgg ttgaaattag
    23881 ttgctactag caaagcaaga aataagatca aacgtttctt caaagcccaa gatcgagaag
    23941 aaaatgtgat caaaggccac gaatccgtgg tcaaatgtat tacagatcta ggatttacgc
    24001 ctaaagatat tttgacgaag aacaaactgc aagaagcact cgatcgtttt aattatcaaa
    24061 cagaagatga tctctatgca gctgtagggt atggagaagt tagccccttg acgatggcca
    24121 atcgtctgac tgaaaaagaa cgtaaagaac aaaaaatcga gcagcaaaag caagaagcag
    24181 aagaaatcat gaatcagccg aaaaaagaac ctgacaaaat gaaagtacgt catgaaggtg
    24241 gcgttgtcat tcaaggggta gaaaacttac taattcgtat cagtcgctgc tgtaatccga
    24301 ttcctggtga tgatatcgtt ggttatatca ctaaaggcag agggatatcc attcatcgtc
    24361 gagattgtcc gaatgttcag cctgacaaac caaatgtagc agaacgtttg attgaagtcg
    24421 aatgggaaga tacatcgaat acacgaaaag agtatgatgc agatttggaa atttacggct
    24481 ataatcgttc aggcttattg aatgatgtac ttcaaacagt caatgcgcta acgaaaaatc
    24541 tcaacagcgt tgaagcacgg acgaataaag ataaaatggc gacgatccat ttgacggttg
    24601 gtatccagaa tttatcccac ctaaagagta tcgtggataa aatcaaagca gtacctgatg
    24661 tctacagtgt acgccggacg aatggatagg aggacagata tgagagcagt tgttcaacgg
    24721 gtaagtaaag ctgctgtgtc agtcgatcag caagaaattg gaaaaattgg ctacggccta
    24781 cttgttttat taggcattca tgaaaccgat acgcaaaaag atgtggacta tctggtaaaa
    24841 aaaattgcac aaatgcgtat ttttgaagat gaacaaggaa aaatgaatat tagtgtagaa
    24901 gatgtgggag gacaaatttt aagtatctct caattcacac tgtttgcgga tacgaaaaaa
    24961 ggaaatcgac caagctttgt ggaagccgca cgaccagaaa cagctattcc tttatacgaa
    25021 tcattcaatg aaggaatcag aagtcgcggc atcacagtcg ctaccggaca attcggagca
    25081 gatatgtcgg tttctctcgt caatgatgga cctgtaacga tcattattga ttctaaaaac
    25141 aaataaagta atctggtttt cactagtaaa aactatgaaa cacgtcgctt aagttagact
    25201 gataaactct aacttaagtg gcttttttgt ctgcaaaaag cgcttctttt gtcagcagtc
    25261 ggaaaaaaag ttaagcttaa attatacaag ttatttttta taggaggttg ctgaagatga
    25321 aagaagtacc agtagtagat atcaaaatca cagatttaaa aaaattgtat caaaaaggat
    25381 acaacatgtt ggaggagtta cgccatgaag cggacgctcc cgtcgtcaaa gcaaagattt
    25441 tcaataaaga agctatcacg atctacggat catcagcagc aaaagtattt tatgatccaa
    25501 ggaatttcaa acgaaaaggt gctatgccaa aattagtatt gaagacactt tttggtcaag
    25561 gtggagtaca gactttggat ggagctgctc atcaccatcg gaaaaatatt tttatggatc
    25621 taatgacacc ggaaagaatg gaagattatc atcgtatctt agataagaat ctgacacaag
    25681 cacttgaagc acagcatggt caatttgaac tatttgattt aagtaagatg gttttcttta
    25741 catctatttg cgagtgggca ggtatcaatt tatcagcgat ttctaaagat gaggtcgaaa
    25801 aattagcaga atatcaaatc tctatgatca gcggaacgtt tacttctccg attgatcaca
    25861 taaaaggtgt cgaaaatcgc aaaaaatcag aaaaatgggc gcaaggcttg attgaagaag
    25921 ctcgacaaaa tcctgtggct ggaaaagaaa atgtggcatt atatgctttt gccaatgcca
    25981 cagatttaga tggtcagctt ttacctctgg aagtggcagc tgttgaatta ttgaacatca
    26041 tccgtccaac ggttgctcta actgtgtggg cggcattgat gggacatgca cttttcagta
    26101 gaccagattt gtatcagcaa ttaaaaaatg acttttcgac gttacaagat ccgtttattc
    26161 aagaaatgag acgctattat cctttcttcc caatgctacc tgcgatttcg ctgaaagaag
    26221 tagaagtaga tggttatcgt ataccagaag gcagttgggt tattttagat ttatatggaa
    26281 cagaccatga tgaacgaaca gttgaagcgc cagattcctt tatgatcaaa cggtatgtag
    26341 ggaaagcaaa agatatttcc tataaagaag aatatgagat gattgcacaa ggcgggggaa
    26401 attttcgaca aatgcatcgc tgtgcaggag aatggatcac tcttcatagt ctacgtgtgt
    26461 tttccgatca gctagtaaat aagtttgagt tctctgtacc tgaacaagat tggacgattc
    26521 cattcaatca gtttccgact tatccgaata gccgagcgct actttacaag aattagatta
    26581 gcataaactt atcgaaaaaa aggaagatca aacagtgctg atcttccttt tttcatatta
    26641 ctataaattg ataaggtcaa ttgatcaaaa tccacgaaat gcttcatcaa tggtttgata
    26701 atcttcattg gataaatgca aatctaaagc ttggacattg tcagatactt gctctggacg
    26761 cttagctcca ggtataacta cagacactcg tggattcttc atgtaccaag ctaaaacaag
    26821 ttgcgcagga gtggcttggt atcttttagc aatggggcga agtacatcga cagcggttaa
    26881 aatttctctg aaacgttctc cttggaaatc tggatttctt tttcgaggat ctccttcacc
    26941 aaattgtttt tcctcaccaa gttcatattt tcctgtcagc aaaccggaag caagaggaaa
    27001 atagggaaca aaactgattt tgtttttttc aagataaggg aataattctt tttcagcctg
    27061 ccgatggatc aaactgtatt tatcttctac tacatcgaca tatccatctg cattcgcttc
    27121 tttcaattgt tctaacgtga agtttgacac accgactgct cggatttttc ctgcttcttt
    27181 taattcgtgc aatgtagcaa ctgattcatt ttttggtgtg ctttcatccg gaaagtggat
    27241 ataaaaaatg tcgatgtaat cagtttgcag acgttttagt gcatcttcca ccgattgttt
    27301 gagaaactca ggagaattat caaatgttcg ccctttattt ggtacatgag cagcttttgt
    27361 agcgatgacg acacgtgagc gatcatattc tttttccttc agtacctcgc caatcagttc
    27421 ttcggaacgg ccgtttccgt aagcataagc ggtatcgatc aaattgatcc cgctattcaa
    27481 agctgtacga actacttgtt ttcctgtctc atcttccaaa ccgtcaaaaa gattatgtcc
    27541 tccaacggca tttgctccta atccaagctg ttctgcgtag acttgtgaat gtccgattct
    27601 tatttctgcc atagctgatt cctccttttt gtctagtcta aaacaaaata ccaaaaagct
    27661 gaactaaaac gtttttgtga ggggaaatag tcagatatct cttgactttg agactggttt
    27721 ttagtaggtt ataagtaata tcgaatgtca atgaaagagc gagtaagttt ttgaaacttt
    27781 acaagagagg atcatcgagg ctgaaaatga tctaaagttt cattagccta aagacactct
    27841 ggagatggac gagtgaaaag ccgtcccgcg tacgagcgtt attcgtaaga ggaaagagaa
    27901 atctttcaaa attaggtggt accgtgtatg aatttcattc acccttgttc atgatgaaca
    27961 agggtgattt ttttatttac atagctaaga aagggagaga aaactatgag tttccaaaga
    28021 ccaaaaggaa caaatgatat tttgccaggc gaatcagaaa aatggcagtt tgtcgaagaa
    28081 acagcacgtt tattattcag agattatcag tataatgaaa tgcgtacacc gatttttgaa
    28141 cactatgaag tgatatcccg aagtgtaggg gatacaacgg atatcgtttc aaaagagatg
    28201 tatgattttt atgacaaagg ggaacgccat gtgacgcttc gaccagaagg tacagcacca
    28261 atcgttcgtt cttttgttga gcataaattg ttcggcccag agtatgcgaa accgttcaaa
    28321 actttttata tgggaccaat gtttcgctac gaacgccctc aaaaaggacg gttacgtcaa
    28381 ttccatcaaa tcggagtaga agcatttgga tcagaaaacc ctgcaactga tgtggagagc
    28441 atggtgatgg cacttgattt cttcaaacag ttggggatca cgcaaatccg gctagtcatc
    28501 aattcattag gtgataaaga aacgcgtcaa gcctatcgtc aagcattgat cgactatctt
    28561 ttaccttttg aatcagagct gagtgaagac tctaaacgcc gccttcatga aaatccatta
    28621 cgcgtattgg acagtaaaga caaacgagat caaaaatttg tggcagaagc tccatctatc
    28681 ttagattatc taagcgagcc tgctcaaaaa cattttgata ccgttgtttc catgctggat
    28741 gcattggctg ttccttatga aatcgacagt aatatggttc gcggactaga ttattacaca
    28801 catacgattt ttgaaatcat gagtgacgca ccaaaaatgg gggcacaagc aacgatttgc
    28861 gcaggcggac gttatgacaa tctagtggaa gaactaggtg gtccttcaac acctggcttc
    28921 ggatttgcaa tgggtatcga gcgattactc ttgactatgg aggcagagga agtagttatc
    28981 cctgcattta atgagttgga tgcttacgtg gtcgcactcg gagacgaaac aaatatcgaa
    29041 gcattgaaag tggtccaagc gattcgaaac tttggttttt cagctgaccg cgacttcatg
    29101 aaccgtaaag caaaagcgca atttaagaca gcagataaag cgaatgccaa attagtattg
    29161 gtgatcggtg aagatgagct tgctagtcaa acgattaatg tcaaagcaat ggcaagccga
    29221 aaagaaaaaa gttttccatt agaagagata tatgaacatt ttgatgaagt ctatgatgaa
    29281 atgactttat tcgagtagga ggaaaaaaat aaatggcaca aagaacgatc tattgcggat
    29341 tagtttccga acaacaatta gaacaaacag ttacattaaa aggatgggta caaaaaagac
    29401 gagaccttgg gggagtaatc tttatcgacc tacgtgatcg tgaaggaatc gttcaaatcg
    29461 tctttaatcc tgaaatcaac aaagaagcat gggaaatcgc ggataaatgt cgcagtgaat
    29521 acgtgatcga agtgactggg gaagttagaa gacgcggaga aaatgcagta aatcctaaaa
    29581 tggccactgg tgaattcgaa gtgattgcaa gtgaaatcgt gattttgaat caagcaaaaa
    29641 ccccgccatt tcaaattgaa gatgatcaag caatcagtga cgaaatacgt ttgaaatatc
    29701 gttatttgga tctgcgtcgt ccaaaaatga ctaaaaacat caaattaaga aatgcgacaa
    29761 caaaagcgat tcgtcattat ttagatgata atgactttct cgatatcgaa acaccttacc
    29821 tagggaaatc tacaccagaa ggcgcaagag actacctagt tccttcacgt gtccatgctg
    29881 gacatttcta tgcgttgccg caatctcctc aattgtttaa acaattgctg atgaatgctg
    29941 gattcgaccg ttattatcaa atcgttcgtt gtttccgcga tgaagattta cgtggagacc
    30001 gtcagcctga atttacgcaa gtagatattg aaacaacgtt cttaacagca gaagaaatcc
    30061 aaacttacac agagggattg attgcgaagg taatgaaaga agttcgcggt atcgaagtca
    30121 cattgccatt tcctcgtatg acctatgatg aagcaatggc tcgctacggc agcgacaaac
    30181 cggatacacg ttttgatatg gaattgatcg atctaagcga tacagtcaaa gaagtagaat
    30241 tcaaagtctt ccaaatggct cttgaaaacg gcggtgtcgt gaaagcattg aatgctaaag
    30301 gcgctgcaga tcgctactca cgaaaagaca tggatcagtt aggtcaatat gtaggacaat
    30361 tcggagctaa ggggcttgca tggctgaaag tagaagaaga cggattgaaa ggtccaattg
    30421 ctaaattcat gggagaagca acagaagcaa tcattaaagc aacagatgca aaaccaggtg
    30481 acttgctgat gtttggcgca gataaaagtg aaatcgtcgc tgctgcatta ggtgctatcc
    30541 ggacacgttt aggaaaagaa ttaggactaa ttgatgaatc gaaattcaat ttcttatggg
    30601 taactgattg gccacaattt gaattttcag aagaagaagg tcgatatgtc tctgctcacc
    30661 atccattcac gatgccaaaa gaagaagata tcccgttatt ggctgaagac cctgcaaaag
    30721 tctatgcaga agcttatgat atcgtcttga acggttatga actaggcgga ggttctatcc
    30781 gtattcatac tcgtgagctt caagaaaaag tactagaaac tctaggcttt acaaaagaac
    30841 aaatggaaga acaatttggt ttcttgttaa cagctttaga ttacggattc cctccacatg
    30901 gcggtattgc attaggattg gaccgttttg caatgttatt agccggagaa gataatattc
    30961 gtgaagtcat tgcctttcct aaaaacggaa aagcagcaga tgtgatgagt gatgcaccaa
    31021 gtatggtttc gcctttacaa ttgttcgaat tgaatattga tgtaacgaat atagaagaat
    31081 agccgataaa aaggtaaaaa ggatttgtcg aatacgcttc ttttgacaac cttgatttaa
    31141 ttgaaaaaca ctttataatg aagccataac atataaagcg gggcttttcc atgaaaaaaa
    31201 cattcgaaaa ttgggcttat catgattaca cgaccaagct ttcggtatct atcgtttatg
    31261 cgttgatcgc atcagtcgca ttgaatttct tttatcagcc aggacatatt tattcaagtg
    31321 gtattactgg tctagctcag attttgacga ctttgtcaaa atcactgata ggattcgaag
    31381 taccggtatc aacaactttg tatttactga atataccttt gtttgtattg gcgtggttaa
    31441 aaatcgggaa gaagtttaca attttcactt ttatcacagt cactttgacc tccctttgta
    31501 tccatatttt accggaaaca gtcctatcta ccgatccaat catctgtgcg ttgtttgggg
    31561 gggctgtgat gggactaggt attgggctag cgttgagaaa cggcctatca tcgggcggtc
    31621 tagatatcgt gagtatcacg attcgtaaaa aaacaggaaa gtcgattggt tctatttcga
    31681 tctacttcaa tgcattgatc gtttttgttg ccggaattct atttggctgg caatatatgt
    31741 tttacagtac gttgtctatt ttcgtcagtg gaaaagtaac tgatgtcgtt tatactaaac
    31801 aaaagaagat gcaggtaatg atcgtaacga aacatccaga aaaagtagta aatgaaatcc
    31861 aaaactgtat gcgtagaggg atcacgatca tcaatgaagc agaaggtgct tataagcatg
    31921 ataagcagac agtattgatg acgatcgtta cacgatacga actgcctcta cttagagaag
    31981 caatgaaagc ttctgatcca caagcttttg taagtattgc agaaaacgtc cagatacttg
    32041 gtcgtttcta tgaggatggc atttagatcg agtttgacaa aaattcatga aatactgctg
    32101 tagacagacg ttcgtttgtc tgcagttttt ttctaccata gtagaatcag caaagaaaaa
    32161 ctgctttttt atttgaaatt ttcttaaagt tgaaaaaaag catgtttatt tttttaaatt
    32221 tatgggatga tagaaaaaaa tagtagagaa aggacacgat tctacttgct attttcactt
    32281 aatacccgta tattacgaat gaaaaaagtt tatgaaaagt tggaatattg aatggaaaaa
    32341 caaaaaaggt taaaaaacag ccgctacatc actgggtttg atgggctccg ttcattagct
    32401 gtggtgggtg ttatcttcta tcaccttctg ccaactagta tgaaaggcgg ctatttgggc
    32461 gttccgattt ttttcgtcgt atctggttat ttgatcacgg atctgctgcg gcaggaatgg
    32521 gaacaaaatg gaaaaatcaa tatttggcaa ttttatgtca gaagaatgaa gagactctat
    32581 ccggcacttg tttttatgct tgtgacagct tcggcttata tcactttgtt tcagcgaggg
    32641 ctgttaaaca atcttcgagg agttgtggtc agcagcttac tgtatgtcaa caactggtgg
    32701 caaatcaata acggtttatc ttatttcgat cgatttgcta atgagtcacc gtttacgcat
    32761 atctggtctt tagcagtaga aggtcaaaac tatttggttt ggccgatctt gtttgtattg
    32821 ttgatggtat ttgttcgtaa aagaaaatgg atcttttata ctgtgttagg tacatccttc
    32881 ctctccgcca tcttgatgat gatcttgtat acaccaggag gcgatccaac ccgtgtttat
    32941 tacggtaccg acactcgtct gttttctata tggttgggaa gtgcgcttgc gtttgtttgg
    33001 ccaagtacgc gtttaaagaa aaacatcccg aaacaagcga aaaatattct gaatatcgct
    33061 ggaggaatct cgcttttggc actgattctt tgcttcttct ttttagatga tcattacaat
    33121 ttcatctatt acggaggaat gctcctagtg agtctgttat gcgtagtgct agttgcagtg
    33181 acagcacacc ccggagcaag tttgaatcga tggctgacaa atccagtatt tacttggatt
    33241 ggcaaacgaa gctatggcat ttatctgtat cagtttccag ttatgatttt ttatgaagca
    33301 aaggtgactg atattgccga tcatgttttg ttgcatactt tagtggaaat cacattgatt
    33361 ctaatcatca gtgaactttc ttatcgatat gtggagcgtc cgctagcacg ttttgattat
    33421 ggtcgtacat tccaagtgat gaaaggatgg ttcagcaaac cgatcatcag taagagcaaa
    33481 ccatgggttg ttccaggcac attacttgta gcaattgcac tcgttggttt cgttacggca
    33541 ccgaaaaacg ctgtaacagc cgatcaaaaa caactgcagg caaaaatcgc tgaaagtaaa
    33601 aaattagcag aagaaaccag aaacaattca gaaaacacca ctagttcggt tgaccaagcg
    33661 atccttgata agtacgagtt gtccaaggca caagggaata aggcacaaaa attagaacta
    33721 acggcatttg gtgattcagt tatgttggat gctgcaacgg atctacaaga actttatcca
    33781 aaagtagtgg tagatggaga tgtcggacga caattatatg ccagtgtacc gtacattgaa
    33841 aaattaaagg aacaagattt gttgaaagac actgttctga tcggactagg aacgaatggc
    33901 tcattctcgg aagctcagtt tgatagtgtg atggatgcaa tcggtgatcg aaaagtatac
    33961 tgggtcaatg tccgagtacc gacgaagcgc tggcaaaatg acgtgaatgc gatgcttgaa
    34021 cagatgacga aaaaatacga caatatgacg ctgattgatt ggtacagcta cagtaatgac
    34081 catagtgact ggttttatga tgatcaagtc catccaaatc ctgaagggat gacacattac
    34141 attcacttag tcagcgaagc cttgctagga ggaagttcaa gtacgaacag cagtgagaca
    34201 aacgtgacag aatgatagct acaaaaaact tcaggcaaaa acatttgttt ttgcctgaag
    34261 tttttttata tttaccgtgt ttttaacatt catatagatt caggaagcaa aacggttgag
    34321 tgtgtctgta aataacgttc tttttgtttt cgggtgagtt ttttgaatgc agcttctgct
    34381 tttgttgctt cgcttcttga agaaaatact tctgcatgga tcatcttaac aggtcttctt
    34441 ttttgaagac gagtatattt agcaccagta ccattgttat gttcctctaa tcgtctagaa
    34501 agatcggttg tatatccacc ataaaatgtc tgatctttac agaataatac gtaaaaataa
    34561 tgttcatttg ccatagagca tctcacgaat ctctgcagtg tattctccat ccatattgta
    34621 agtgatcaat ggtggagcga ctttgaatcc gtctaatttg ccttctttga ttccttcaac
    34681 taacaaaata tttgcctctt ttccaggttt tgggtaaaca aattggatcc ttttaggtgc
    34741 aattcgatag tcttccatga cctgaatgat ttctaagaac cgatcaggac gatggaccat
    34801 agtaaagcgt ccatttgttt ttagtagctt acttgaaata tcgatcactt cttctaacgt
    34861 cgtatgaatc tcatgacggg cgattgccag gtgtttatta gggttcgttt tatttgttgg
    34921 cagccctttg aaataaggtg gattacagac gactaagtca caagaattat gtcgaacgac
    34981 agataagctg tctttcagat cgatggtgtg gacagtcatt tgttcattta aatggttaag
    35041 cgcgatacta cgtcttgcca tgtctgctag acgaggttga agttcgatag cgtcgatagc
    35101 cgcctgggtt cgtttactta aaaaaagtcc aactgcgcca tttcctgcac aaaggtcaac
    35161 aatcttccct tttttaggta cacgggaaaa gtgagcaagc agcacagcat ctaaagaaaa
    35221 agaaaacact ttggaacttt ggataatctt gatatcttct gcgtataatt ggtcgattcg
    35281 ttcgtcttcg tgaagcatgg attccctcca aaatgaaaaa aatagtcgtc tgtattatac
    35341 tatttttatt aaaaagaacc aaggtggctt gtcaaacctc tattttttat gcatactatg
    35401 accataagaa gggaaaggaa gagactatgt tttatcgatt tatgcgcggg gtagttcgag
    35461 tactattagc cttagttaat ggaaatgccc gctatgagaa taaagaagtt ttaccgcaag
    35521 atgagaatta catactagtt gctccccatc gtacttggtg ggacccatta tatctggcgg
    35581 tagctgcagc acccaaggaa ttcagcttta tggcaaaaga agaactgttc aaaaatccag
    35641 ttctgcgatt tatattaact cgatccaatg cttttccagt taaaagagaa aatccaggac
    35701 caagcgtcat taaaacacct gtaaagattt tgaaatcgac aaatttgagt ttgatcatgt
    35761 ttcccagtgg gacaagacat gctacagaac ttaaaggcgg aatggcattg atttccagaa
    35821 tgtctaaagc gaaaatcgtg ccagctgtat atcaaggtcc gttgacactt ggggatctgt
    35881 tcaagcgtca gagagtgaca gtgcgtttcg gcgaaccaat cgacttatca gatatcaaga
    35941 aaacggataa agaaggtatg gcggagatcg aacgacgaac acaagcagct tttgacaaat
    36001 tggacaaaga agtgaaccct gatttcaaat atgaagtcaa aaaaaaataa aatgataagt
    36061 ttatgcgtgg gcggtctttt gaatctactg tcctgttcag ttcagacttt ttcaatgaaa
    36121 acgagggaga gacgtcaagt ctgccctctt tttgcgttcc aatatgtaaa atagaagaca
    36181 ggtgtcatgt aatggatcaa agtgttataa taattagaag gacatttttt gaggtgattg
    36241 aatgcgtttt ttacatacag ccgattggca tatcggaaaa aaactacaag gttatgactt
    36301 gattgaagac cagcgtcatg tactatcttc tatactagat atcgcgaaag aagaaaaagt
    36361 agaggcaatc gtgattgccg gagacttata tgataggagt gtcccagctg ttgatgccgt
    36421 agaattatac aatgaattga tggtcgattg gaatttaaag gaaaagatcc cgatttttgc
    36481 tatatcagga aatcacgata gcagtacgag actttcagcg ggtacgccat ggttcagtta
    36541 ttcgaactat tttttgcgaa cacagctagc agaagcattt gaaccagtcg agtttaaaaa
    36601 cgtacaattt ttctttcttc cttactttga accaattgca gcgagattat attttgaaga
    36661 tgacaatatc cgaacgatca agcaagcggt agccaaggtg atagataaaa tgcaggagct
    36721 atttgattca gaaaaaaaac aagtattgat tagccatttt tttgtaatgg gaagtgtaaa
    36781 atctgattcg gaaacgaaaa tcgaagtagg cgggttggat tcgatccctg gtgaactact
    36841 tacaccgttt gattatgtag cactaggaca tttgcataat caagctgctt tgagacagga
    36901 taatgctcgc tatagcggtt ccccattgaa attctcttta tctgagatca accaaaaaaa
    36961 aggcgtatgg atcgttgacc tgacaacagg cctggagttg aattttaggg agatcgaacc
    37021 cctgcatgat attgaagaaa taacagcgag tttcaaagaa ttgctggatc ctgcctttta
    37081 tcagtccgtc aatcgggaga attacatcta tatctatctc actgatagaa cgatcatccc
    37141 gaatatgatg aaccagcttc ggcaaatata tcctcgtatt ttggggattg aacgggcaaa
    37201 cggtcgagca gaacaaacaa agcgcaaacg aaaagtacta gaaatgaaag atcctcaaaa
    37261 attagcagaa gcatttttta gtgaaatgac ggaagagcag ctaactgcaa accaagaaaa
    37321 atggctcgga gaaacgttgc aagaaatcat ggaaatggaa tgatcaagaa tgaaaccgaa
    37381 aaaattacaa cttaaaaatt ttggtccttt tatcaatgag accgtagatt tttcacgttt
    37441 gacagaagca ccgctttttt tgatcagcgg gaagacagga gctggcaaaa cgacgatttt
    37501 tgatgggatc accttcgcac tttttggtga aacttccgga agacttcgct ctggaaaaga
    37561 gatgcgttct ttgtttgcta caccagacga agaaacaagc gtgacttttt cttttgaaca
    37621 tcagcagatg agctatgaaa tcgaacgaaa accagaacaa gttttagcaa aacgaaaagg
    37681 aaacggaaca aaaaaacagg cagccaaagt aacgttgacc atttttgatg ggaaaggaaa
    37741 agaaataaaa caaatcacaa aacgtacaga agcagatcag ttgatcagag aactgatgaa
    37801 tctcgatgcg aaacagtttt ctcaaatcgt tttactaccc caaggagaat tccgtaattt
    37861 tttggtttca tcaagcagtg ataaagagac tgttttacgt catttattcg gtactcaatt
    37921 tttccaacag ttcaatgagc gattaaaaga aaaagcaaaa caacagcaac aaaaattgga
    37981 tcatttagag caagagctgc atcttctgca aaaaaggttt gttttagtca aagaagagga
    38041 accagcagca gggtttgaag ccgttttagc ccaatggaca aaccatcagg caatactcga
    38101 aaaacaaatc acggaagaaa aagaagctct aacggaactt caggaaaaac aaaagcaact
    38161 agaaaattcc tactatcgtt ttgaaagctt gaaaaaaagt tatgaagaaa aaacgatatt
    38221 aataaacaag caaaaggaat tagctgctca aaaagacgaa atagaggaaa agaaacgatg
    38281 gattgggtat tatgaattta cgcaacgatt agtcgaacca atccagcgcc tacaagaaat
    38341 aaaagttgaa aaaatagaac tgtcacaaaa aagtagtgag caaacaaaga aactagcgat
    38401 gactacgcag aattatgaag agtggcaaaa aaatgaagca aaccgcgtac gccgacaaac
    38461 aaaaatcaaa gaagctgctc aaaaactgca aaacaaacag atgatggtgc caattgtcga
    38521 atccttacgt agtaaaaaac aagacgcaga acaattaaca atttcattgg ataaagaaga
    38581 attgaatgcc gagaagctgt atcaaaatct cgctatctat caggaaagaa ctcgaattct
    38641 tcaagaaaat ttactggatc aggaaaaact gcaagaagat cgtcttgctt ttggacagtt
    38701 aaaatatcta gatgaaaagc agcaagaaat agaaaaacaa aaagcaaaag acactgggtc
    38761 gctcgcacag ataagagaag aactcaaaaa agcaactgaa gaaataacaa aaaatagtca
    38821 gctattgaaa gaacagacag aacaagcaga acagctgaaa agccaatggg caaagcagca
    38881 aattgctcgt cttcagcttt ttttgcgtcc aggggagcca tgtcttgttt gtggatcaac
    38941 ggaacacccc atacaacaat taaaacacga acagccaaat aaggaagata tcttaaaagt
    39001 agaacaacaa ctggcagaag ctgagtctgc tgtagaaaga actcagcaaa tcattgccaa
    39061 aagtgaagca catgaaaata atcttcaaca acaattatca gatttagaac aaaaaatcat
    39121 ggcaacgaat atacaattgg ctgaacaaga agaacacttg caacaggctt tgaaaaaggc
    39181 atttcctgag ataacagaac aaaaaacaaa aacaactctt gtagtaatcg aacaatcttt
    39241 agatagacga aaaagcaaga tggccaatgc aagaatagag ctggaagaaa taagacaaca
    39301 agcagaaaag ctcgaacaac aattagctgt ctacaaacag cagcagacag aaaaaaataa
    39361 tcagctccag caactgcaag gagaaatcaa aagtttagaa caacaattgc aagatacgga
    39421 tagtaactgt ttagaagagg agatacgacg acttgaaaaa cagttagaag aagactcgaa
    39481 tcaactagct gttgagcaga aagcaggagt gcaattgttg caagatcttc atacgcttcg
    39541 gacacagctt gatcttcaac atgaacaaat agaaaaaata gatgaaaaaa tagaaaagga
    39601 acaagaaaga atagctaaaa agttagaaag ccagtcgtat ttttcaacaa ttgaagaaat
    39661 ctgtgcattt gcacaacagc aagcacaata taaagaaaac caacgtacag ttacttctta
    39721 tcatgaagag caattagtga cagctgaccg gctgcgtcaa ttagaaaata tccagctaac
    39781 tgagacattc ccagatacag aagagatcag ccaacagtta gaagaaataa gacgtatgat
    39841 cgaaataaag caagaaaaga tatacggttt acaagaacaa aaaattagta atcaaaaaat
    39901 ctacgatgaa tgtctttcga tttatcacac aagccagacg aaattagatg agcttacgca
    39961 attacagcag ctttcgcaaa cattcaacgg agaaaatcct aagaagacta gtttggaacg
    40021 gtatgtgctt caggtttatc ttcaagaagt gttacaagta gcaaatgatc atctccaacg
    40081 tttaacgaaa aatcgttatc aatttgagct agcggatacc attggcagtt atcgagggaa
    40141 aaccggatta gaaatcaata tctatgatga tcaggcaggt atgtctcgaa gtgcgcatac
    40201 cctgtctggc ggtgagagtt ttattgcagc actttcttta gcattagctc ttgccgatgt
    40261 gatccagaca caagctggtg gtgtagcaat cgatgcattg tttattgatg aaggatttgg
    40321 gtcattagat gaagaagctt tggaaatggc aatggaagct ttagaaacga tcgaaagtga
    40381 aggaagaatg atcggcatca ttagtcatgt aagagaattg aaagaacgag tattacaaca
    40441 aataagaatt gatacagaag gtagcggaca aagcaaaata cgctatcaac ttggataaaa
    40501 agggagattg gtaactatga aatggagtat tccagaaaaa gtcattgcac gtggacgtca
    40561 atacttgcaa gaaggccgag tgttatcggt tgtccctgac caagaagaaa acgtgtggca
    40621 tgcagaagtc ttgggcagtg aattgtatat ggttgatttg gatggtacag caaaagagat
    40681 agattactgc caatgtccct attgggagga acataaatat tgtaagcata cagttgctgt
    40741 ggaactttat ttacggtcga aaaatctatc tcgtatcatg aaaaaagacc agccaatgct
    40801 agaaccggta aaagcaacaa gtgagggaga aatcattgca aaaggttttt ctcgtctaaa
    40861 aacacaaaac aatcctcaag cggtgcaacc tttgattatt gaataccaag tagaaacgat
    40921 tgagactaat cagtatcacc cagagctttc tattttggca ctttacttgc gtatcggttc
    40981 attggcatca ccgaaaaaaa catatatcgt aaaaaacatc tatgattttt tacaagctta
    41041 cacagaaaaa gaaacttaca ctgttaataa acagtaccag tttcgtctag ataaacgggc
    41101 gtttcaaaaa gaagatcaaa aaatactcac gcatcttgca ggaagtgctc agacgcatca
    41161 attattaggt acgaacggct tacaagtcaa aggaaaacta gataaaagat atctgcttct
    41221 cccagtagaa caagcccgct tcctgttaga gcagatgaat cagactacac gtttgcatct
    41281 gcagataaat gaacaaaacg atcaaggaat acaattttcc gatcaaaaca aacctttatc
    41341 attcaaagtg gaaaaatttg gagaagatta tttgctgcaa gcattgaatg attttgattt
    41401 tttctttatg cattatcagt ggggagttat ccaagggaca ttttattctc tgacgaaata
    41461 tcaacaaacg gtttatcaaa cttggaaaca attgattcgc cgattggaga aaccagaagt
    41521 agtgttcaag aaaaaagaat taccgtcttt gtttaaagag attatccctc tgctgtcaga
    41581 gatcggcgac gtagctattg cccctgcagt agaagaggaa gtagcagata tccccgtcga
    41641 atttattttc tttttccgaa aagcaaaagg aaaaatccaa gctcgagtcg atttcgtcta
    41701 tggcgaagtc atctattcga ctgacccgaa gcatgaggtc tcgacagaac aggcttttca
    41761 ggtgttacgt gacacagtag aagagaaacg agtacttgat ctattcagaa tgtatcgcta
    41821 ccagaaaaat gaaactggtt atgaacgtcc attgccaagc ggggaagaac tatatgcctt
    41881 tttccgcaca gaactatctg tcttccgtca gtttggtgaa gtcagacttg gaagaaaatt
    41941 gcgagagttg tatttagatg cgcataaaca tcaaccgaaa atcgaagtaa ccgagaccgg
    42001 ttcatggctc gatattcgat tcgatgtgac agggatagag gagcaagaaa tcgatcatgt
    42061 cctgcaaagc ttgctgagaa atgatgcttt ttatacatta gaaaatggtc aggttctttc
    42121 ttttgattca gaagaatttc aacaaacaag ccaagtgttg caacaattac gggaatcgat
    42181 acgtacagaa gaaggaacga tccatgtacc taaaaatcaa ggattgatca ttcaaaatca
    42241 attggaaaaa agcaatgcta cttttagtga atcgttccaa acgatggtac aagatctcat
    42301 tcatccagaa agataccagg cacagcttcc caaaggattg aatgcaacga tgagagatta
    42361 tcaaaagcaa ggttttcgat ggttgaagat gttaggtcat tatcagtttg gcggtatttt
    42421 agccgatgaa atgggacttg ggaaaacact acagacaatt gcctttttac tttcggaaaa
    42481 agaagaacgg aaaagcttct ctgctttgat cgtagcgcct gcgagcttaa tttacaactg
    42541 gcaggcagag gtgagaaaat tcgccccttc tttaagtatc caagtaatca atgggaataa
    42601 aaaagaacgt gaagaattac tggctaaaga cacagatatc cgagtgactt cttatgccag
    42661 cttaagacag gatctagcta attatcagtc gcaaaaaatt gattatttga ttcttgatga
    42721 ggcacaaatg gtcaaaaata gcagtacgaa aactgcacaa gcattgagag aattagctgt
    42781 acctcagcgg tttgcactga gtgggacacc aatcgagaat aatttagaag aattgtggtc
    42841 attgtttgca acgattatgc caggattttt cccgactaaa acaaaattca gggaacttgc
    42901 cgcagaagaa attgcgcaaa tgattcgacc gtttatttta cggcgagata aacaaactgt
    42961 gttgaaagac ttgccggaaa aaacagagat gaatctatat tctgcactta cagaagaaca
    43021 aaaaactgtt tatttagctt acttgcgtca aatgcgtgaa gaaatcacat caatggattc
    43081 tgaagcattc aagaaaaacc ggatcgggat acttgctggt ctgacaagac tgcgacaaat
    43141 ttgctgcgat cctcgtctgt ttattgaaga ttaccaaggc ggttccggca agctcgaaca
    43201 agtaaaagat ctactggtag ctgccaaaga gaacaaacga agagttttgc tgttttctca
    43261 atttacaggg atgctgacta tcttgcaaga agagttagcc gagctaggga tctcgacatt
    43321 ttatttaaga ggcagtacga aaccacaaga tcggttaagt atggtagacg catttaatgc
    43381 tggcgaaaaa gatgtttttc tgatttcctt aaaggctggg ggaactggat tgaatctcac
    43441 gggagcagat acggtcatct tgtatgacct ttggtggaat cctgccattg aagaacaagc
    43501 tgctggacgt gcacatcgga tcggacaaaa aaatgtggta gaagtgtggc gtatgatctc
    43561 tgaaggaacg attgaggaac gcatggattc actccagcaa gaaaaacgcg aactgttcca
    43621 aaaagtgatc caaggtaatg aagaacagtt gacgaaactg actgaagaag acatccgcat
    43681 gattttaagt gtaggagaaa tcgaggagta agagactcga aaaagcgttc agtaatgggt
    43741 aagaaagcct ttctaaagaa gcgattgttt atttgattta aaaaatagca aggaggctgg
    43801 gattccattt tatcccagct tccttgctat cattttttta tttctatttt taaaggttat
    43861 acaaagattg tcttcattgt ctctaacagc tgatcaaaag aagaaatctc gatatcgcaa
    43921 attggagtct gttcaatttt gcgttctcga tggttgaacc aaagcgcttg ccagttagca
    43981 ctttttgcac ctagaacatc attgtcgtaa ttatcgccaa cgtataaggt ctcttctgga
    44041 agcatatgaa attctttctc agctagctga aaaatctctt tttctggttt ttggaaacct
    44101 gttgcttgag aaatgagcat attgcgggaa ggaatccaat tgttcaattg aagttgcttc
    44161 agtttttttg tctgatggtc agtcggccca tttgtgataa ttcctaatgg gacattcttt
    44221 tccttcaaga aatcgagggt ttttttgacc tcctcatgca agcagatatt atcaagttct
    44281 tcctcgtaaa ttttttgaaa taagaggcca tcttcttccg taatatgagg gtagtctaaa
    44341 tcttttagtg attggctgat ccgatgtgct cgcatatatt ctagtgtcca atctcctgcc
    44401 attacttttg ggaagttttc atcactatga tggcggaacc ggatatatag tggatgcata
    44461 tctgcatctg aaacaagcgg aacgactctt ttgaccgcgt ttcgaaatgg ctgctgctga
    44521 tcatacatcg tatcatcaac atcaaacaca actgatttaa tcaaaatttt acacgccttt
    44581 cggttttcat tgattttttc acaaatcctt gactattgta acagaaaaaa ttgtctaaaa
    44641 aagctgataa ataaaggttt ttcttactat atttctattt ccttgacaga ataaggtttt
    44701 tatatttcag tttttttcac taaaaatcgt gaaacacgtc aataagtttg atgaacaaat
    44761 taatcattat ggtagaagtc gattgattta ttttaatgca atctctgtgg aattttcg
//
LOCUS       DANKKG010000008        43756 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000008 DANKKG010000000
VERSION     DANKKG010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43756
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.008"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            558..3197
                     /locus_tag="PW004_000381"
     CDS             558..3197
                     /locus_tag="PW004_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDL2725379.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(3365..4063)
                     /locus_tag="PW004_000382"
     CDS             complement(3365..4063)
                     /locus_tag="PW004_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HDL2725380.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            4281..5426
                     /gene="tgt"
                     /locus_tag="PW004_000383"
     CDS             4281..5426
                     /gene="tgt"
                     /locus_tag="PW004_000383"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HDL2725381.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            5523..5903
                     /gene="yajC"
                     /locus_tag="PW004_000384"
     CDS             5523..5903
                     /gene="yajC"
                     /locus_tag="PW004_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDL2725382.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            6208..8805
                     /gene="adhE"
                     /locus_tag="PW004_000385"
                     /gene_synonym="adhC"
     CDS             6208..8805
                     /gene="adhE"
                     /locus_tag="PW004_000385"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDL2725383.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            9034..10140
                     /locus_tag="PW004_000386"
     CDS             9034..10140
                     /locus_tag="PW004_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HDL2725384.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            10157..11185
                     /locus_tag="PW004_000387"
     CDS             10157..11185
                     /locus_tag="PW004_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HDL2725385.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLEGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            11191..11670
                     /locus_tag="PW004_000388"
     CDS             11191..11670
                     /locus_tag="PW004_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HDL2725386.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            11686..12981
                     /locus_tag="PW004_000389"
     CDS             11686..12981
                     /locus_tag="PW004_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HDL2725387.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            12992..13816
                     /locus_tag="PW004_000390"
     CDS             12992..13816
                     /locus_tag="PW004_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDL2725388.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPETP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            13981..14880
                     /locus_tag="PW004_000391"
     CDS             13981..14880
                     /locus_tag="PW004_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2725389.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(15006..15875)
                     /locus_tag="PW004_000392"
     CDS             complement(15006..15875)
                     /locus_tag="PW004_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HDL2725390.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            16028..17488
                     /gene="rhaB"
                     /locus_tag="PW004_000393"
     CDS             16028..17488
                     /gene="rhaB"
                     /locus_tag="PW004_000393"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HDL2725391.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            17485..18741
                     /gene="rhaA"
                     /locus_tag="PW004_000394"
     CDS             17485..18741
                     /gene="rhaA"
                     /locus_tag="PW004_000394"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HDL2725392.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            18755..19600
                     /gene="rhaD"
                     /locus_tag="PW004_000395"
     CDS             18755..19600
                     /gene="rhaD"
                     /locus_tag="PW004_000395"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HDL2725393.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            19602..19913
                     /gene="rhaM"
                     /locus_tag="PW004_000396"
     CDS             19602..19913
                     /gene="rhaM"
                     /locus_tag="PW004_000396"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HDL2725394.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            19995..21146
                     /gene="fucO"
                     /locus_tag="PW004_000397"
     CDS             19995..21146
                     /gene="fucO"
                     /locus_tag="PW004_000397"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HDL2725395.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEVSVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            21273..22031
                     /locus_tag="PW004_000398"
     CDS             21273..22031
                     /locus_tag="PW004_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HDL2725396.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIQTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            22131..22775
                     /locus_tag="PW004_000399"
     CDS             22131..22775
                     /locus_tag="PW004_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HDL2725397.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            22782..23789
                     /locus_tag="PW004_000400"
     CDS             22782..23789
                     /locus_tag="PW004_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDL2725398.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            23805..24647
                     /gene="kduI"
                     /locus_tag="PW004_000401"
     CDS             23805..24647
                     /gene="kduI"
                     /locus_tag="PW004_000401"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HDL2725399.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            24667..25470
                     /locus_tag="PW004_000402"
     CDS             24667..25470
                     /locus_tag="PW004_000402"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HDL2725400.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(25719..26783)
                     /locus_tag="PW004_000403"
     CDS             complement(25719..26783)
                     /locus_tag="PW004_000403"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HDL2725401.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(26780..27865)
                     /locus_tag="PW004_000404"
     CDS             complement(26780..27865)
                     /locus_tag="PW004_000404"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HDL2725402.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(27878..28855)
                     /gene="mvaD"
                     /locus_tag="PW004_000405"
     CDS             complement(27878..28855)
                     /gene="mvaD"
                     /locus_tag="PW004_000405"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HDL2725403.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(28848..29792)
                     /gene="mvk"
                     /locus_tag="PW004_000406"
     CDS             complement(28848..29792)
                     /gene="mvk"
                     /locus_tag="PW004_000406"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HDL2725404.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            30108..30767
                     /locus_tag="PW004_000407"
     CDS             30108..30767
                     /locus_tag="PW004_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDL2725405.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            30852..31757
                     /locus_tag="PW004_000408"
     CDS             30852..31757
                     /locus_tag="PW004_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDL2725406.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(31759..32391)
                     /locus_tag="PW004_000409"
     CDS             complement(31759..32391)
                     /locus_tag="PW004_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDL2725407.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     misc_feature    32542..32667
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            32711..33235
                     /gene="infC"
                     /locus_tag="PW004_000410"
     CDS             32711..33235
                     /gene="infC"
                     /locus_tag="PW004_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HDL2725408.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            33307..33507
                     /gene="rpmI"
                     /locus_tag="PW004_000411"
     CDS             33307..33507
                     /gene="rpmI"
                     /locus_tag="PW004_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HDL2725409.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            33560..33919
                     /gene="rplT"
                     /locus_tag="PW004_000412"
     CDS             33560..33919
                     /gene="rplT"
                     /locus_tag="PW004_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HDL2725410.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            34171..35508
                     /locus_tag="PW004_000413"
     CDS             34171..35508
                     /locus_tag="PW004_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HDL2725411.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            35556..37685
                     /locus_tag="PW004_000414"
     CDS             35556..37685
                     /locus_tag="PW004_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HDL2725412.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            37660..38349
                     /locus_tag="PW004_000415"
     CDS             37660..38349
                     /locus_tag="PW004_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725413.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(38757..39370)
                     /locus_tag="PW004_000416"
                     /pseudo
     CDS             complement(38757..39370)
                     /locus_tag="PW004_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(39506..39700)
                     /locus_tag="PW004_000417"
     CDS             complement(39506..39700)
                     /locus_tag="PW004_000417"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725414.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(39750..40190)
                     /locus_tag="PW004_000418"
     CDS             complement(39750..40190)
                     /locus_tag="PW004_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HDL2725415.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(40194..41039)
                     /locus_tag="PW004_000419"
     CDS             complement(40194..41039)
                     /locus_tag="PW004_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HDL2725416.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            41188..42606
                     /gene="pepV"
                     /locus_tag="PW004_000420"
     CDS             41188..42606
                     /gene="pepV"
                     /locus_tag="PW004_000420"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HDL2725417.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(42663..43610)
                     /locus_tag="PW004_000421"
     CDS             complement(42663..43610)
                     /locus_tag="PW004_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL2725418.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
BASE COUNT        13834 a         7969 c         9470 g        12483 t
ORIGIN      
        1 aaaaagccga gctctcgata agctcggctt tcttgtacct cggaaggatt caaataaaca
       61 gaaatgattg taactttcca tcatacatgc aaagcacggt gttgaatttc ttcatgttaa
      121 acaaaaattt tgttttcgtt acatcttcaa catttgaaac atctcttgtt cctattcaat
      181 tttcagacgt ttacgttatt caattgttac gtatcgcatt acttcttgtt tcggatactt
      241 cgtgatccaa acatccatac gcattcacta tttataggtt tgtactctac acgcatgtct
      301 gggtgaaacc tgtacttcta acaactttgt ttgcttacac tgttctatcg agaaattctt
      361 ctgtctattt gatggtatcc tccttcctta catctatata atagcatcct ttggtagcgt
      421 ttgcaaatat aagcacttta aattttacgc tttcttaaca aataatcgat aagcccttta
      481 tttgaagtaa ataaaaaaag gatgttatag taaaataagt accttttttt gtacgaagat
      541 tttaaaggag gaataggatg gaagaataca agcagacaat agaagaagta aagaaggtaa
      601 cagaagcaga tccagatact gggttgtctt cacaagaagt ggatgaacgg cgaaaaaagc
      661 agggattgaa taaattcgat gaggcgccga aagaatcgat gatcaaaaaa ttctttcgaa
      721 gcttgtccga ttttacaacg atcatccttc ttgtagcagc ggtgatttcc ttttataccg
      781 catttgctac agaacatggg gatttatttg aaggactgct gatcatcgct atcgttgtga
      841 tcaactcggt attggcgatt gtccaagaag gaaacgcaga aaaagcactg gaatccttgc
      901 aagatatgaa taaacaaacg gcaactgtca tacgagacgg caaagtgacc actgttgagt
      961 cagagcaact ggtagtgggt gatattcttg ttttagagtc cggtgatgct atttctgccg
     1021 atgcccgttt gatcgaagca tcacaattac gtgtagaaga atcggcgttg actggagaaa
     1081 gtgaagcagt tgaaaaagat gcagcattta ttgctgaaga agatgagtca ttaggtgacc
     1141 aacttaatat ggtatttaaa ggatgtacgg ttgctgcagg tcgtgggaaa gcgatcgtca
     1201 ctgccattgg catggctaca gaaatgggta agatcgcgga tcttttgaac gaaaatacta
     1261 tgcaaaaaac acctttacag gtccgattga atcagctagg caaacgaatc agtatgatcg
     1321 ctttagcggc tgcggcttta gtatttgtga ttggtgaact gcaaggagaa ccactccttg
     1381 agatgttcat gacttcgatt tccttggctg ttgcagctgt accggaaacc ttgactgtta
     1441 ttgttacgtt gacacttgca tacggtgtgc agaagatggc gaggaaacat gcgatcatcc
     1501 gacagttgcc agcagttgaa acgctaggaa ccgctaatgt tatctgctca gacaaaacag
     1561 gaacgctgac acaaaatgaa atgcgcgtcc gccgggtatg gagcaaagaa gatgaagtaa
     1621 cgaatatcga agattcaatg acgaacagcg cgatggaaat attgaaaatg gctgcactat
     1681 gtacagatgt gacggtcgag caagaagatg acgaccttgt tatcaaaggt aatccaacag
     1741 aagtagccat cgtaagggct gttgaagaga attatcatac aaaggcagaa ttagaagaaa
     1801 aatatccacg agtaaacgag cttccttttg attctgaacg aaaaatgatg acaacagttc
     1861 accaaatggg cgaaaaatac atctcaatca ccaaaggagc attcgatgtt cttgctccgc
     1921 gctttagttc aggcgatgta gaacaagcgg cgatcgttaa tgatcgcttt ggtaaacggg
     1981 cactgagagt gattgctgtt ggttatgcta cttatgacga agaaccgcaa gatatttctt
     2041 ctgaagcact ggaaaaagac ttacgtttga ttggtctgat cggaatgatc gatccaccac
     2101 gtccagaaag taaaggagca atcaagcgag cgaaaaaggc tggcatcaaa acagtcatga
     2161 tcactggaga tcatgtggtg actgccagtg ccatagcgaa agaattaggt atcttgaatg
     2221 atccgtcaga agcactttca ggatcagaac tgcatcaatt atcagacgaa gaattagatg
     2281 cgcgtgtcaa agctctctct gtctatgctc gggtaacgcc agaagacaag atccggatcg
     2341 tcaaatcttg gcaacgtacg ggtgcagtcg tagcgatgac tggggatggt gtgaatgatg
     2401 caccagcgtt gaaagcaagt aacgttgggt gtgccatggg gatcacagga acagatgtgg
     2461 cgcaaagtgc cgcagatatg attttgacgg atgacaactt tgcgacgatc gtagacgctg
     2521 tcagtcaagg acgttcggtt tatcagaata ttcgtaaagc tatcaacttt ttgctaagct
     2581 gtaatatttc ggagatcttt attgttttga ttgcgatgct gttaggatgg ggagcaccat
     2641 ttacagctgt gcaactactg tttgtcaatg tagttgctga tggacttcca ggatttgctt
     2701 taggaagaga gccagcggaa cacggcatca tggatcagcc acccattccg aaaaatgaag
     2761 ggatctttgc tcgaggttta ttgcaaaaaa ttgccataaa tgcaggggtc ttcaccatcg
     2821 taaccttgtt tggttattat ttaggtagtt atgtcgatac tatcagccca tgggtagacg
     2881 caagtcagca tgtcggtcaa actgttgcat tcttgatctt agcttattca tcgattctgc
     2941 atgtgttcaa tgtacgcagc agccaatcga tcttcaaagt caatttagcg accaataaag
     3001 ctttggtaga aatggcgtta ctggcattag ctattacaac ggctgttgct ttgctgccat
     3061 ttacgcaaga actattcggc ctagtacaca tcagtttgaa tcactggttc ttggtaggga
     3121 ttttatctat cgtaccaatc gcggtgaatg aactgatcaa gttccatcga ttaccggaag
     3181 cagaagaaga ggaatgatta actgtttcta agttgatttt ctttttactg gaactataca
     3241 aaaaggaggt gtgacatgaa tgttgtcaca cctccttttt gctaatgggc tgaatgtttc
     3301 ttttactgcc tctcgataaa cggtgttctc ctgccacccc ctcggtcaca agtcacactc
     3361 tcttttaaat atcttttggc aattgttcat aaaatgctgc ttttgttgca gaaatgtatg
     3421 ggctgagcca tagataacca ataccagcag ttgctaatgc taagatatgc cagccgataa
     3481 atgaaagatc taaccagaaa agttgtcctt tataaccatc catcaatttt cgacttgcag
     3541 tgatcgtatc cagtacttta ggtgtttgtc ctgtttcttg gacgatatcg tagtaaataa
     3601 aatagctttg agaataagaa tagcttttaa tgattccagg aataactaat agcaaacccc
     3661 ataatgttgt aaagatcgag atcagcacag ctaagctgat cactccgccg ataaacaaac
     3721 ctttgaatcc acgaaatgca tctttgaatg gttcgatctg atctttttgt ccacgtaaaa
     3781 tatcaagata agtccaagaa ataccgctca tgaacaaagc gctaatgatg ctggaaataa
     3841 ttccgccacc gcctcctgaa ctaccgcctc ctgaagttgt cgcttctgag acgctcgctg
     3901 atccatcata tatggtggtg gcaaacaaga taataggaat cataataaga agaatagtag
     3961 caataccaat caatgtaggg attagattta gtaaaacagc ttgtccccaa cgtcctttta
     4021 gactctcttt tgcttggcgt tttaattctg cagaagattg catgattttt ccctcctttt
     4081 ctttaataac accaatatac catgtatcaa aaaatatttc tgtcataatg tttttctttt
     4141 ttttgttatt cataaaaaaa ggataaaaaa aagaatatta aataaagtag aaggaagccg
     4201 agggaacttg caaaaagcac ttgaaacgat tatgatggtg ggagttgtca agatatttta
     4261 tatagaaagt agggaaatga atgacgcaac ctgccattcg ctatcgttta atcaagaaag
     4321 aaaaacatac tggcgcgcgt ttaggggaat tgatcacgcc acacggaact ttccccacac
     4381 caatgttcat gccagtagga acattagcta cagtaaaaac aatgtcccca gaagagttga
     4441 aagaaatggg tgctggcgtt attttaagta atacatatca tttatggcta cgccctggtg
     4501 aagacttagt agaagaagct ggcggactgc ataaatttat gaactgggat caaccgattt
     4561 taacagattc aggcggcttt caagtttttt cattaagtga tatgcggaat atcgaagaag
     4621 aaggcgtgca tttcaaaaat cacttgaacg gttcgcgtat gtttttatca ccagaaaaag
     4681 caatcaacat ccaaaataaa ttgggctctg atatcatgat gagttttgac gaatgtccac
     4741 cattcgatga aagttacgac tacgtgaaaa aatcagtaga acgtacgtct cgatgggcag
     4801 aacgaggatt gaaagctcat gctaatccag accgtcaagg attatttgga atcatccaag
     4861 gagcagggtt tgaagatctt cgtcgtcaaa gcgcgaaaga tctagtcggg atggatttcc
     4921 ctggttactc aatcggcggg ttatctgtcg gggaacctaa atcagaaatg aatcgtgtat
     4981 tagaattcac tacaccgttg atccccgaaa acaaaccacg ttacttgatg ggtgtcggtg
     5041 ctgctgattc attgatcgat ggtgttatcc gtggagtaga catgtttgac tgtgtcttgc
     5101 ctactcgaat cgctcgaaat ggcacatgta tgacttctca aggccgtcta gtagtgaaaa
     5161 atgcgaaata tgcccatgat ttccgtccaa ttgatgagaa atgtgattgt tatacatgta
     5221 aaaactatac acgtgcctat atccgccatt tgatcaaatg tgatgaaaca tttgggattc
     5281 gtttgacttc ttaccacaat ttatacttct tgttgaatct aatgaaacaa gtacgtcaag
     5341 caatcatgga tgataatttg cttgaattta gagaagcctt ttttgaagaa tacgggttca
     5401 ataaagaaaa cgcaaaaagc ttctaaagga aaatctttta aaaattaagt aaaaaactag
     5461 tgttatcagc atttttctgc taaagtgttt tttgtagata cagaattagt gaggtgaaaa
     5521 aaatgggaac attaccaatg cttgtgatgt ttatcgcgct attagcaatg tggtttttta
     5581 tgtcacgttc gcaaaagaaa caacagcaag aacgtcaaaa cttgttagat agcatgaaac
     5641 caggtgattc agtcgtaacg atcggcggat tacatggagt tgtttcagaa atcgacaatg
     5701 acaaacggac agttgtttta gactgcgaag ggatcttcct tgagtacgat cgtgcagcaa
     5761 tcaaaacggt caaaccaggg actgtcgtaa caaatgatgc aacgactact gttgaaacaa
     5821 cagaagaacc agctgagaca gttgaagtaa aaactgaaac aacagaaacg ctggaagaga
     5881 cagaagagaa ggaagaaaaa taagtatcaa agcttattta aagacgaggt agggcagaaa
     5941 gatgtcctac ctcatttttt attcaaaaaa gaaagagata agagcatggg atatattgct
     6001 caatattgaa taatcgggtg tgtcatataa ataataataa ataactttat atatttttct
     6061 atacaaacac ctgtaaataa agcaatttta tatttaaaaa taactgtgaa aaaaatcaca
     6121 aaaaagtgtt tacatagaaa aataaatatt gtatgatata catgtgaatt atttaacaaa
     6181 taaccatgac ccattaggag gataatgatg gctaaagtaa tagaaaaaga aaaaacgaag
     6241 acaattgacg tacaagcaat gattgatgat ttggcagaaa aagcaaatgc agcattgaaa
     6301 gaaatggaaa gctttgatca agaaaaagta gaccatatcg tgcatgaaat ggcaatggca
     6361 gctttggatc aacatatgcc attagcgaag atggctgtag aggaaacagg ccgcggtgtt
     6421 tacgaagata aagcaatcaa aaatatgtat gcgtccgaat atatttggca taatatcaaa
     6481 catgataaaa cagttggtgt gatcaatgaa gatgtccaaa aagggttgat tgaaattgct
     6541 gaaccagtcg gtgtggtttg cggagtaact ccaacaacga atccgacttc cacaacgatt
     6601 ttcaaatcaa tgatcgcgtt gaaaacaaga aatccaatcg tttttgcttt ccatccaagt
     6661 gcccaaaaat cttctgcaga agcagcacga gtggtacgtg atgctgctat cgctgccgga
     6721 gctcctgaaa attgtatcca atggatcgaa catccttcga tcgaagcgac ttctatgcta
     6781 atgaaccatc caggtatcgc gatcgtctta gcgacaggtg gagctggaat ggtcaagtct
     6841 gcttactcta caggaaaacc agctttaggt gtaggtccag gtaacgtacc agcttatatt
     6901 gaaaaaacag caaaaattaa acgtgcagtg aacgatttga tcgtttcgaa aacatttgat
     6961 aatggaatga tttgtgcttc tgaacaagct gtgatcgtgg ataaagaaat ctatgcagca
     7021 gtaaaagcag aattccaagc acaccaagtt tatatcgtaa aaccagatga attgcaaaaa
     7081 ttagaagatg cagtgatgaa cgaaggaaaa tatgcggtca atccatcaat cgttggacat
     7141 tctgcgatgg aaatcgcaaa attggcagga atcagtgtac caaaaggaac gaaaatgctg
     7201 attgctgaat tggaaggcgt aggcccagat tatccgctat caagagaaaa actttctcca
     7261 gttcttgcca tgatcaaagc taacaacacg gaccatgcgt ttgatttgtg tgaaggtatg
     7321 ttgaatcttg gaggattagg acatacagca gttatccatt cagaaaacga agagttgcat
     7381 gtcaaattcg gtcttcgaat gaaagcttgt cgtattttag tgaatacacc atctgcagaa
     7441 ggcggcatcg gtgatatcta taacgaaatg attccgtcat tgactttggg atgcggttct
     7501 tatggtaaga actctgtttc tcgtaacgtc tctgctgtaa acttaatcaa tgtcaaaact
     7561 gtagcgaaac ggagaaataa catgcaatgg ttcaaattac cgccaaaaat cttctttgaa
     7621 aagaactctc tattgtactt agagaaaatg gaaaacgttg aacgtgtcat gatcgtttgt
     7681 gaccctggta tggtccaatt cggttactgc gatacagttc gtgaagtatt ggcacgacgt
     7741 aaaaatgatg tgaagatcga agtcttctca gatgttgaac cgaacccatc gacaaatacc
     7801 gtatacgcag gaacgaaact gatggcagac ttcaagccag atacagtaat cgctcttgga
     7861 ggcggttctg caatggacgc agctaaggga atgtggatgt tctatgaaca cccagacaca
     7921 tctttctttg gtgcaaaaca aaaattcttg gatatccgta aacggacata taagatcgac
     7981 aaaccagaaa aaacacaatt cgtatgtatc ccgacaacat cagggactgg ttctgaagta
     8041 acgccatttg cggtcatcac tgacagtgaa acacatgtga aatatccgtt agcagattat
     8101 gcattgacgc cagatgtcgc aatcgtcgat cctcaattcg tcatgtcagt tccagcatct
     8161 gtcacagcag ataccgggat ggatgtgtta acacatgcaa tcgaatccta tgtttcagtc
     8221 atggcttcag attatacacg tggattgagc ctacaagcaa tcaagttagt ctttgactat
     8281 ttagaaaaat cagtaaaaac acctgatatg gaatcacgtg aaaaaatgca caacgcttct
     8341 acaatggctg gtatggcgtt tgctaacgca ttcctaggta tttgtcactc agtcgcacat
     8401 aaaatcggcg gggaatacgg gatccctcat ggacggacaa atgcgattct tttaccgcat
     8461 attatccgct ataatgcaaa agatccacaa aaacatgcga tgttccctaa atatgactat
     8521 ttccgtgcag atacagatta cgcagatatc gcaaaattct taggtctgaa aggcgaaaca
     8581 acagaggaat tagtcgaagc tttagcaacg gctgtttatg aattaggaaa atctgtcggc
     8641 atcaacatga gtctaaaagc tcaaggagtc acacaagaaa cactagatac aaccgtagac
     8701 cgtatggctg aattagctta cgaagaccaa tgtacgacag ctaatccgaa agaaccattg
     8761 atctctgaat tgaaacagat catcattgat gcttacaacg gataagcaaa agaaagcgta
     8821 gaaaaaagat aagaaaatag atgaaaaaga acttggaaaa cgttgtttct ccaagttctt
     8881 tttttaattt ccaagtaact gaaagttact tttgttattt aacatttagg tcaattttag
     8941 acagtttttg ttcaaaaagc gatatttata gtggacggta ttactgctat attaaggata
     9001 taaaatgaaa gcgtttaaaa aggtggcgta aagatgatta ttaaagaaga gatttttgta
     9061 cctaaagaat tagtgaaaga aaaaattgac ctgctcgttg aaaacctgac aacgatcaaa
     9121 gacgataatg gtgaattttt gttggacttt gatggactga aagtagatga caaaagctgg
     9181 acagtgtgga attggccaca aggtgtcggt ctatatggta tttataaaaa ttaccagatg
     9241 acaaaaaatc ctcgcgctta tcaagtggtc aatgaatggt ttgaggatcg tatggaagaa
     9301 ggcgctccgc ccaaaaacgt caatacaatg gcaccgctat tgactatggc ttacttgtat
     9361 gaagacacga aagattcacg ctatttgcct tatttagaac aatgggcaga atgggtcatg
     9421 aatgacatgc ctcggacaaa tgaagacggg ttacagcatg ccacatatgg accggaaaat
     9481 aagaatcagt tgtgggacga tacattgatg atgacagtgc ttcctttggc aaaaatcggg
     9541 aaactgttga atcgcccaga atatttagaa gaagctcgtt accagtttat gatccatatc
     9601 aaatacttga tggataaacg aacaggattg tggtatcacg gctggacgtt tgaaggaaat
     9661 cataactatg ccgaagcatt ttgggctcgt ggaaactgct ggttgacgat tgctattcca
     9721 gagatcattg aaatcttaga attatcgaaa gaagatgcat tgcgcaaatt attgatcgaa
     9781 acacttgaag cacaagtgcg tgcattaaaa acttatcagt ctgaaagcgg tttatggcat
     9841 acgttgttgg atgatccttc ttcttacgta gaatcttctg caactgcagg atttgcttac
     9901 ggtattttga aagcggtcca taaacgttat ttaccacaag aatataaaga agtcgcttat
     9961 cgtgctatcc aaggattgct tgaacaaatc gatgaaaaag gagaagttca aaacgtttca
    10021 atcgggacag gaatggggga cagtttagat ttttatcgga atatcggaat cacagcaatg
    10081 ccttatggac agtcgttaac ggtgctttgt ttgacagaat tgctcgtctc ttattgttaa
    10141 aagaaaggaa acccatatga aaaaaacatc actgctgttt gcagcaagtt cgcttagcct
    10201 aggtctgcta ggtctaagcg gctgcggaaa taattctagt gtggcaactg tcgcagatgc
    10261 agatgtaaat gagatagtta ccttgcgctt tgcttatgca agcaacagcc agccagtaat
    10321 tgatgcaatg aatgaatttg ggcgtttagt aaaagaaaaa acagatggtt ccgtacagat
    10381 ccagtatttc ccagatggac aactgggtgg agaacgtgaa ctgatcgaat tgacacagtc
    10441 aggagcagta gatttcacaa aagtaagtgc ctcagcgttg gaaagtttct ctaaagatta
    10501 ttcgattttt tcagttcctt atctattcaa tgatgaagaa catttcttta aggtgatgga
    10561 taatgaagaa ataatggagc caatctacca atcgaccaaa aatttaggat ttaccggatt
    10621 gacctattat gattctggtc agcgcagctt ttatatggta gacggtccga tccatacgcc
    10681 ggaagatttg gagggaaaga agatccgtgt gatgcaaagc gaaaccgcta tcaaaatggt
    10741 agagctttta ggcggttcgc ccgtacctat gggaagtgat gaagtgtaca cgtctcttca
    10801 atccaatttg atcaacggat cagaaaacaa tgaatttgtc ttgtatacag ctggacatgg
    10861 tggtgtggct aaatactatt cctatgatga gcatacacgt gtgccagata tcatcatcat
    10921 gaatgatgcc atcaaagaac agctaactga tgagcaggaa aaagccattg aagaagcagc
    10981 gaaagaatca acggtttttg aaattgaagc ttttgcaaaa gcaattgaag aagaaaaagc
    11041 tttagcacaa gaagaacatg gagtccaatt caatgatgta gataatacac cgtttcgaga
    11101 ggcagttgcc ccacttcatg atatattcaa aaacgatcct acctacaaag acctttatca
    11161 aaagatccaa gagcaaggag cgtagctgtg atgaaaatga ttttaaacaa ggtattagag
    11221 atcctgggag ctgttttgct tgttgggatg gtcgctgttg ttttatggca agtattttcc
    11281 agaacggttt taggaaatcc aaatacggtc acggaagagc ttgttcgatt cggtcttgtc
    11341 tggtttgcca tgttgtcttc cgcgtatgtt gtgggacaaa aagcacattt agccgtaacg
    11401 gttttaagtg aaaaattaac tggcaaaaaa agtaaaattt tagaagttgt tgttcaaagt
    11461 ttatttctgg tatttgctgc gatgatcatg gtgtatggcg gatggaatgc tgtcacattg
    11521 acaatggggc agatctcgcc ttctttaagt attcctatgg gatatgttta tctgtcagta
    11581 cctgtatcgg gggtactcat cattatctat agcctgatca atctaatcga tacattaagc
    11641 aacaagccag caattcaaga aaaaatataa aaatggagga ataaaatggc aatagaagcc
    11701 ggattagtgt tattttttgt atttgttgtg ttgcttgtat tagggatgcc gattgctata
    11761 agtattgcat tctcatcgat gatgacttta ttgttagtta taccgtttga tgtttctgct
    11821 tttagttcag ctcaaaaaat ggtgggaagt atcaatagtt tttcgctgat cgctattccg
    11881 tttttcgttc tctcgggcat tatcatgaat aatggaggga tagctaagaa actagtggat
    11941 tttgcgatgt tgttcgtcgg gcgcgtgccg ggggctttgg ctcatacgaa tgtgttaggg
    12001 aatgctttat ttggttccat ctctagctcg gccattgccg cttctaccgc gattggcgga
    12061 gtgttgatcc ctcagcaagt ggaagaagga tatgaccgga agtttgcgac agcggtcaat
    12121 atcgcgtcag ctccgacagg tatggtcatt cctccaagta ctgcttttat catgtattca
    12181 cttgttgctg gtggtgcttc gatctctgct ttgtttatgg gaggttatct cgtaggcgct
    12241 ttgtgggcgc taggaatcat gctagtagca ttcatcgttg ctaaaaagaa taaataccct
    12301 gttgtcgaaa aaggacagat ggctcatgct ggaaaaataa taaaagaggc gattcctagc
    12361 cttctattga ttgtcatcat tatcggtggt atcttgacgg ggatatttac agcagtagaa
    12421 gcttcagcga tagctgtcat ttactccctg ttgattgcta tgttttacta taaaacggta
    12481 aaactaaaag atatacctaa tatgttgttt caatcagtag caatgtcagg gacgatcatg
    12541 ttcttattgg ctacttcttc catgatgtct ttcgccatgg cgtttaccgg tattcctcag
    12601 gcaattagtt cgttgattat gggcgtaacg gataataaat ttattattct gttattagtc
    12661 aatcttgtgt tattactggt gggtatgttt atggatgtgg cgcctgctat cttgattttt
    12721 acaccaatat ttttaccaat cgtcacaagt gtaggggttg atcctgtaca ttacgggttg
    12781 ttttcaatca tgaatctgtg tgtaggctcc atcacgccgc cagtaggtac aggactatat
    12841 gtcggtgcaa gtgtaggagg cgtaaaagca gaggcaatgc tgaaaccgct tcttcctttt
    12901 tatggggtta tcttagtcgt cttgtttttg attacttact tcccgcaaat cgtcatgtgg
    12961 ctacccaata tgctgagtta ggagaatagt tatgttgaac attacaatta gaggacatga
    13021 tctttccgaa gtccaaacga tagaagatct tgcagaaaaa acaaaagaac aagggattca
    13081 tacactgcag ctcgccttag gtctttcgtt tcctgaaaca ccttctggcg caaatgagat
    13141 caattctggg atgggaaatt atgtcaaacg agttttagaa aagcaagaag tgaccgtagg
    13201 aattttaagt tgctatatca atatgattca tccagattta acgatacgac aagagttgct
    13261 gacaaagttc gagcagtatc ttcgctatgc ttcttccttt ggtgcgtcga tggttgcttc
    13321 ggaaactgga tgtgttttgc cggaaatcca atatacggaa gaaaatttca cagatgaagc
    13381 atttgctgaa gctgtgtccg ttatccgtcg cttggtaaaa gctggagaaa aataccaaat
    13441 gatggtgggg atagaaccag gtttgaatca tccggtgtat tctttagcaa gggtagagca
    13501 gctgatccaa gcagtagatt cggactatct aggaatcatt ttagatccga ccaatttgat
    13561 cacaagtagt gattatcaag aacaagtcca attggtagaa gaagcttttg aacgttttgg
    13621 tgaaaagata tgtgcggttc atttgaaaga ttttcgtgtc gaacaggaaa aaatcgtgcc
    13681 ggtaaatttg ggagacggtg tgatcgagta cacaaaaatc aaagaaatca tcatgaagaa
    13741 tcgtccgtac ctgtatgtgg ttcttgaaga aacaaaagat gatgggattc gttatggaag
    13801 aagtcttctt gaataatgta aagaagacgt tggttaacta atatttagaa tggagggggc
    13861 ggggctagaa gatgccacgt ctcttctttt ttataagagt atataaaagc agctcttttt
    13921 gattacaaaa aacgtggtat aataaaattg tgcataagtg aacaaaagga ggtgcggcac
    13981 atgctccaaa agaaaatcag cggtgaacaa gtcaatacga tggtttctga tcagtatgaa
    14041 atctatcatg tcaaagatgt cgtcagccaa aataaaacga tctatcatca tcatgatttc
    14101 tatgagatcc atgctacatt aaagggagaa gcgacttttt atttgaacgg acgacaattt
    14161 acgatcaaag caggaagtgt gctgctcatc cattccaatg atttgcatcg tatcgtccgc
    14221 caatcttcag atgtattcga gcgtgtctat atctttgtca ccccttcttt tctagaaagt
    14281 cgttccacgc catggtctaa tctatcggct tgttttcaac caatgggaga gaagaggagt
    14341 cgtgtattgc aaatagaaca ggaagtattg aagcaaaaat tatcatttat cgaccatcct
    14401 cctgataaaa aaatttacgg tgaagacata aaatatgaac aagctctggt agaatacttg
    14461 atttttctca atcaagttgt acaaaaagaa gagagtgcgc aagaacagaa tgctgtagtt
    14521 cctaatgagc gtgtggaaca aatgttacaa tatatctcaa aaaatttatc tcatccgctt
    14581 tctttagagg aaatggaaaa gcagtttttt gtcagcaaat accatatcac acgagaattt
    14641 aaaaagtaca ctggatttac gtttcatcag tatgtaatga agaaaaagct gatctatgcc
    14701 aagcacttgt tgcgagaata ccgcagctct agtgctgtat atagtaaaag tggtttttct
    14761 tcttatccgc attttctgaa agcattcaag agagagttcg gcataacgcc taaagaattt
    14821 ttgaaacgtg atacacagaa acagttggtt cattatgaac attttgaaga tgaacaatga
    14881 gaatgatcac taaaaaataa ccgcagtagc tgctgttgtt tacatagaag cagaaactgc
    14941 ggttatttta tttttatcta tatatttcaa aatattttga aaaaatgacg tacatattat
    15001 atgaatcagc tgaatttacg aaagtctctc ggcgatagtc ctgtttttgc tttgaacagg
    15061 cggctaaagt aagcaaaatt ctcaatccct atttcttcgg aaatcattcg aaccgtaaat
    15121 tctgtatacc gaagaaggtc tttagcgtaa gaaagttttt tgctgatcaa ataatcgatt
    15181 ggtggagaac cgataaattt ggaaaaatct ttgctcagct gatatttatt caataagaat
    15241 tgttgttcta attgatcaag tgtaatcgtt tctttgaaat gttcgtctaa gtattgcttc
    15301 agtttgtaaa cgtaggcggg aatatcctct tcttcgaaat ctagctgata cttttgcaaa
    15361 atcagttcat tcagcaactc gtggatagct acagaggact ggaaatcggt acgggcattc
    15421 gtgccttgtt gcatgtggat caattctgtg atcaaatggt ggatcttgtt ttctgatgga
    15481 tcccctgttg aatggaaggt gttatggccg tcttttgtga actcctgata aaatgattgg
    15541 acgttctgac cattcaaatg gatccaatcc atttcccatg gttcttcgct gatcgtttga
    15601 taacgctgat attcttggca gtcaataaaa aataagtctc cagcttttag ctgttgctga
    15661 ttatcattat atgtaagcga acctttgcca gacaaagtaa actttatcaa gtaagaaggt
    15721 aaatgtgctc gttcagtata gtaagggatt ggtgctttga agtaaccgat ttcttggata
    15781 taaaaaaata aattcctgct tctttgtgta ggcgtcaaga tcactctttt tgagtcttcc
    15841 gaccatgagt ggttggcttt ttcccagtaa tccatgtaaa cactcccaac ttgtcaagat
    15901 aatgcaatta tataccaaaa tagtgtgaag caaaatataa gattctgtat tatgatagca
    15961 ttgttaataa atgataacgc tttattaata aagtgctaat caacaataaa gtaaaagagg
    16021 tggagaaatg aatccaacat ttgttgccat cgatatcggt gcatcaagcg gacgactaat
    16081 gaaaagtacg agggatgcaa aaggttcttt gatcttggaa gaagtacacc ggttcaaaaa
    16141 cggctttcgg aacaaagacg gtcacgatcg ctggaagatc gattatctta tcaaagagat
    16201 tttgattgga tttgaaaaat taaagcagac agggattgat cgcctttttg cggggatcga
    16261 tacatgggca gtcgattact gtctgattga tgaacaaggg gatcggataa atgatccgat
    16321 tgcttatcgg gacaagcgta ccaatggagc agtagaacag ttttctgaaa tctattctct
    16381 agataagttg tatgagcaga cagggattca gatccagcct ttcaatacct tgttccaatt
    16441 gtttgctgaa gaaaaagaaa atttggcaaa agcacataaa ttgttactga tacctgatta
    16501 tttagggtat gtctttactg gtaacatggt gacggagaaa actaatgcat ctactatgca
    16561 gctgctgaat gcagggacaa aagaatggga aatagaactt cttgaaaaaa tcggtgtctc
    16621 gaaggaatta tttccaccat tgacagagcc gggaacgata ctaggagatc tgcaaaaaga
    16681 acaattttca aaatatgatt tgccagatgt gacctttatc acagtagcaa gtcatgatac
    16741 ggcgtcagct gtattaggaa cgccaggtca aggagaacgt tggggttata tcagcagtgg
    16801 gacatggtcg cttttaggga ttgaaacaaa gatcgccaat gtttctcgca atgctttttc
    16861 tgaaaattat acgaatgaat ggggtgccca taacacggtg cgttttttga aaaacatcat
    16921 ggggatgtgg ttgatccagg aagttgcccg aatgcaagag tacgagtaca gttatgcgga
    16981 attggcagag cttgctgcaa aagaaaaacc gctgcagcaa gtgatggaaa tcaatgaccc
    17041 tcgatttttg aatcccccaa atatgataga agagattcag caatactgtt ttgaaacaga
    17101 gcagccagtc cctgtctcat cgggagctat cgctcgttgt gtgtatgata atcttgctct
    17161 ttgttacgca caagagctga tcaaagtaga acacttaact ggaacgaaat tggaaaagct
    17221 gcacatcgta gggggcggat cgaataatcg tctactgaat caattgacag cagattatgc
    17281 cggaatcatt gtagaagcag gacctagtga agccacagca atcgggaatg ttgccatgca
    17341 aatgctcagt acaggtcagt tttcgaatat agaacaagca agaaatgtaa tcaaacagtc
    17401 gtttccatgt gaaacttttt acccagatga aaataagcaa aacacgatgg aatggtacgt
    17461 gaataaaatc aaaggagtaa tcgtatgaac acgattgaaa gaaattacga acaagcaaaa
    17521 gaaaaatatg caactatcgg tgtagataca gatgctgtac tagaaaagat acaggacatc
    17581 aaaatttcta tgcattgctg gcaaggagat gatgtcaaag gtttcttgac accggatggc
    17641 gaattgaccg gtggaatcat ggcgacaggg aattttccag gagccgcacg aacacctgaa
    17701 gagcttagac aagatctgga gaaggcttat tcgttgatac cgggcaaaca caaattgaac
    17761 cttcatgcta tctatttgga tacagaagaa tctgtcgatc taaatgaaat tgagccgaaa
    17821 cattttgaaa aatgggtaga gtgggcaaaa aaagaagaaa tcggtcttga cttcaatccg
    17881 actttctttt cgcatccaat gatgaaagac ggctttacat tggctcatcc agataaagaa
    17941 gttcgtgaat actggatcga acacgggaaa agaagccgca aaatcgcgga gtactttggg
    18001 aaagaaactg ggcaaacaag tatcaataac ttttgggtac ctgatgggat gaaagataat
    18061 ccgattgatc gttttacccc acgggaacgt ttaatggaat cactcgatga aatttttaca
    18121 gaagagttag atgaacggta tgtacaagat gcagtagaaa gcaaactatt tggtttaggc
    18181 gctgaagctt atacagtcgg ttcccatgaa ttttatatgg gttacgggtt gactcgtgac
    18241 aaacttgttt gtctagatgc tggacatttt catccaacag aagtgatttc caataaatta
    18301 tcttcattag ctttattcag caaaggaatc atgttgcatg tttcccgtcc agtgcgttgg
    18361 gattcagacc atgtggtttt gatggatgat gaattgcagg aaatcgctaa agaattagtc
    18421 cgtaacgaat tgttggaaaa aacaaatatc ggtctggatt tctttgatgc aacgatcaac
    18481 cgtatcgctg cttgggtcat cggtacgaga aatacacaaa aagcactgct gaaagctatg
    18541 ctggaaccag ttgagcgatt gaaagaaatg gagctggctt ttgactttac ttcaagaatg
    18601 gcttatacgg aagaattgaa agatttcccg tatgccgatg tatggaatta tttctgttac
    18661 aaaaatcaag ttccagttgg tttggattgg ctggaagaag tccaggaata tcaaaaagac
    18721 gtgttggaat taagaaaata ggagtgtggc tatattgaaa aaaatagata ttttacaagc
    18781 gccttatgtg aaagaaatga tcgaaacgac gtcgaatctt tatcgcttgg gatgggatga
    18841 aagaaatggc gggaatattt cttatttact aaaagaagaa gagatcacac cgttcttgga
    18901 tcctgaaaaa gtgatccgcc gcatctcgat gatttttgat gccagccgtt tagcaggatg
    18961 ctattttatt gtaactggtt caggaagata ttttaaaaat gtagcgagcg aacctgccga
    19021 gaatcttgga atcgttcgag ttgctgaaga tggcaaaaca ctggaacttt tgtgggggct
    19081 ggaaaacgaa tgccttccta caagcgagtt gccaagtcat ttcatgagtc atatcgcccg
    19141 tttagaagta gacccagata atcggatcgt tatgcattgc catgctagtc atctattagc
    19201 gatgagtttt acgcatgaat tagacgaacg ttcctttagt cgtacgctat ggcaaatgtg
    19261 tacggaatgc ttagttgttt ttccagaagg aatttccatc attccgtgga tggttccggg
    19321 aacaaatgag attggggaag caacagctga aaaaatgaaa gaaactcgat tggttttatg
    19381 gccacaacat ggaatctacg ggtcaggaaa agacatggat gaagtctttg gtttgatcga
    19441 gacagcagaa aaggcagcag aagtctatac gtacgtcaaa gcgcaaggac caatcttgca
    19501 aacaatcacg gacgaaaacc tgtggcattt agcagatgcg tttggtgtca ctccaaaagc
    19561 aggttatttg gaggaagttc atacgaaagc aggtgtctga catggtgaaa aaagcttttt
    19621 gcatgaaagt ttacccagaa aaacaagaag aatacaaaaa aagacatgat gaactttggc
    19681 cagaaatgcg tacagcactt cgtgaacacg gggtcatcga ctattcgatc tatcttgatc
    19741 cgagtacaag tttgttattt ggttacttag tgatcgaaga tgaaaagtta tgggaaaaaa
    19801 tggcaaatac atctatcaat caaaaatggt ggaagtacat ggaagatatc atggagacaa
    19861 atccagatgc ctctccagtc agttgggaat tgatgccagt ctttgaactg tagcaacaag
    19921 caactgggca accggttgct tggggttctt tcgtttttat tgtgaaaaaa aatacaaatt
    19981 aggaggaata gaaaatgagt aatcgaatga ttttaaatga aacatcgtat tttggaaaag
    20041 gagcgattga gtcgatcgtc cctgaatttc agcagcgagg attcggcaaa gcagctgtca
    20101 ttactgacaa aggattgatc gagcacggta tagcaacaaa agtaacagaa cttctagata
    20161 aagcagcaat cgcttatgct ttatacgacg ggatcgtacc taatccaacg atccaaaatg
    20221 taaaagatgg agtggctttt gttaaagagg cagaggcaga ctgtttgatc gcaatcggtg
    20281 gaggttcgcc aatcgatacg gcaaaagcca ttgggattat tttgacgaat ccagagtttt
    20341 cagacgtcat tagtttagaa ggtgttgctg atacgaaaaa tccatgttta cctatattag
    20401 ctataccaac gacttctgga acagcagcag aagtaacgat caactacgtc atcacagatg
    20461 aagtaaatca ccgaaaattt gtttgtgttg atcctcatga tatcccaatc gtcgcgttca
    20521 tcgacagcga tatgatgatg ggaatgccga aaaaattggc tgcatcaaca ggtatggatg
    20581 ctatgacgca tgctattgaa gggtttatca caaaaggcgc ttgggaaatg actgatatgc
    20641 tgcatttaaa agcaatcgaa atcatcggcc attctttgga agtttctgta gatggcgatc
    20701 aaaatgggcg agaaaaaatg gcattaggac agtatattgc gggaatgggc ttctcaaatg
    20761 tcggtttagg actggtacac gggatggctc acccgttaag tgcatggtac aacattcctc
    20821 atggcgtagc gtgtgcagca cttttgccaa ctgtcatgaa atataacaaa gaatatactg
    20881 gagaaaagta ccgtgagatc gctcttgttc tagggattaa aggagcagct gaaatgtctt
    20941 tagaagatgt aagagaagcg gcttgtggtg agatcgatcg gttgagcaaa gcggttggaa
    21001 tcccagaaac catttctgaa ttaggtgtaa aagaagcaga tattccagca attgcagaag
    21061 atgctttgcg agatgtatgc acaccaggaa atccaagaga aacgacagta gaagaaatca
    21121 ttgctttata tcaaagtttg atgtaaaaac gaagcggatc ttcaacatta aattgcctgt
    21181 aaagagaaat ggtagtttat atctggatat ctcttttaaa ttgatggtat ttccgctata
    21241 atttttctta taaagaaata ggagaatatt atatggaaaa taaaaagcct tatggaactg
    21301 tattgatcaa agcctctaaa atactagact atttagcgga aaaccaagat gtctctttgc
    21361 agacgatcgc aaagggtgtg ggaatgacct cttctacagt cttgaaaata ctagatactt
    21421 tgttgatgat cggctacgta aataaaaatt cagagaaaaa ctatcgttta ggtgcaaagc
    21481 ttattcgcta cgcgaataaa aacattgaac aaatcgattt ggtagagttg actttaccgt
    21541 ttttagaaca gctgcagcaa aagatcgatg agactatcca cttaggggta ttggacaaca
    21601 atgagatctt gtacgtgaac aaactagatc caaaacatca aacgatccgc atgtcttcaa
    21661 aaatcggaat cacccgacca ttatacaatt ctgcgatggg caaagccgta ttagcagaat
    21721 tcagtgaaga gcagtacact gattatcttg aaaagcatcc gttgatccct tacacagaat
    21781 atacgatcac caatgcatta cggttgaaaa aggaaatcca aacggtaaaa gaaacccaca
    21841 tcgcatttga cgatgaagaa atcgaacgtg atattttttg tataggagcc tcaatcgtga
    21901 aaaacgaaca aatcattggt gcatttagtg taagtatgcc gaaatatcgt ttaacagatg
    21961 agacaaaaga acaaataatc agtgccctca agcaaacaaa aagcgaaatc gagcaaagaa
    22021 ttcaaaaata atgaaaaaag cgtttgcaat ttttcttttc tgccgtataa tatgggtata
    22081 gatttccaag ttataaaata aaatttctaa ataggaaata ggtggagaag atgaaaaaaa
    22141 ttgatatttt atctcgattg aaaaacgccg gagtgattgc ggtggtacgc ggaaaaagca
    22201 aagaagaagc tttgaatgct tgccacgcaa tcatcaaagg tggtctaact ggtattgagt
    22261 tgacttttac agtccctcaa gcagatcaag taatcaaaga acttttgtca ttctacaaag
    22321 accaaccaga aatagtgatt ggcgcaggca cagtattaga cgctgtcaca gctcgcttgg
    22381 ctatcttagc tggcgcagag tatattgtta gcccttcctt tgatcaagaa acggcagaga
    22441 tgtgcaatct gtatcaaatt ccttacttac caggatgtat gactattacc gagatcaaaa
    22501 cagcgctgaa aagcggggta gatatcgtca aactatttcc tggaagcgct tatggaccaa
    22561 gtatcatttc tgccttcaag gcgccgatgc ctcaagtcaa tatcatgcca actggcggtg
    22621 tgagtctgga caatatgaaa gagtggttcg atgctggtgt cgtaacagtc ggagtaggtg
    22681 ggaacctttt agcacccgca gctacaggag attttgacaa agtaacggaa gtcgcgcaac
    22741 agtatgcagc aaaaatgaaa gaaatcaaga ggtagctgct tatgggaaaa gtagtgacac
    22801 tcggagaaat catgttgcga ttgtccacag agcagggaac ccgtatcgat tcaagtgaac
    22861 gattcgctgc ccattatggc ggaggggaag caaacgtagc aatttcttta gctaattacg
    22921 gacatgaagc gatttttgct acaaaagtac ctgataacgc attgggaaaa gcagtcaaaa
    22981 agcacttgca atgttatgga gtagatacga aacaggtatt atctggtgga tcaagactag
    23041 gtacttatta tatggaaact ggtagtggcg aacgcgccgc ctcagtgatc tatgatcgtg
    23101 ccggatcaag ttttgctgaa atggatcatt tggaatggtc gctagaggag ttattccaag
    23161 gaatagatat atttcatgtt tcaggaatca ccccagcttt gtctaaaaaa tggcaaaaaa
    23221 tgacgaaaca ggtgatagaa gctgcaaaaa aagctggctg cctgatcagc ttcgatatca
    23281 actatcgtgg aaaattatgg acacagcaag aagccagcga agtaatccat cagcttttac
    23341 ctttagtaga tatctgctca gctggaaaaa tggatgctct ttatttgtta ggtatcgaga
    23401 aagcgccaga agcagaagaa caacctctaa tttattatta tcaaaaaatg caggaaaagt
    23461 tcccgaatat tcaggtgttt tattcgacca agcgtaccgt tcattcagca agtgaaaatg
    23521 aattgaaggg gacattgtgg atgaaccatg aatatgtgga atcccaagtc catagaatca
    23581 ccccaatcgt ggatcgagta ggtggcggag atgcttttgc aggtggcgtg ctccatggga
    23641 ttttatccaa tatgtctccg caagaaatca ttgattttgc tacggcagct tctgccttga
    23701 agcatacgat ccatggggac tgcaaccaat ttagtcaaga agaagtcgca caatttttag
    23761 catgcggctc tggaaaaatc attcgataga aggagtagaa gaaaatgcaa gaaatggaaa
    23821 cacgttatac acacagtcca gaagatatcc gtcattattc aacggaacaa ttgagaaaag
    23881 aatttttagt agaaaaggta tttgaaccag ggaaaatcag tttgacttat acgcacaacg
    23941 atcgaatgat ctttggtgga gtgacaccta ctgaaaaaga attagaaatc acattagaca
    24001 aagaactagg tgttgattac ttcttagagc gtcgtgaact aggtgtgatc aatatcggtg
    24061 gaccaggatt catcgagatc gatgggcaaa aagaagaaat gaaaaaacaa gacggatact
    24121 atatcggaaa agaaacacga catgttgtat tttcttctca agatgctgaa aacccagcta
    24181 aattctatat tagttctgtt ccagctcatc ataaatatcc aaatgtaaaa atcagtatcg
    24241 accaagtaaa accaatggag actggtgaag gcctgacact gaatgaacgt aaaatctacc
    24301 aatacatcca tccaaatgta tgcgaaagct gtcaattaca aatgggttat acgattttag
    24361 aaccaggaag cgcttggaat acgatgcctt gtcatacaca tgaacgtcgt atggaagctt
    24421 atgtctactt tgattatgcc aatgaagata cacgtgtgtt ccacatgatg ggcaaaccag
    24481 atgaaacaaa acatttagtt gtagacaatg aacaagccat tatttcgcca agctggtcga
    24541 tccattcagg tgtaggtaca agcaattact cattcatctg ggcaatgtgc ggagaaaata
    24601 tcacctatac agacatggat atggtgcaaa tggatcaatt aaaataagca taagggagag
    24661 aaaatgatgg aatttagtat ggatatgttt cgtttagatg gaaaagtagc attagtcacc
    24721 ggtgctgtct atggtattgg atttgagatt gcacgctctt tagctacagc aggagcaaaa
    24781 atcgtattta ataacttgaa tcaagaatca gttgatgaag gtatagcgca ttataaagaa
    24841 gcaggcatcg atgcaaaagg ttatgtctgc gatgtgaccg atgaagaagc ggttcaagcg
    24901 atggttcaac agatcaaaga agatgtaggt tcagttgata ttctagtcaa caatgcaggg
    24961 atcatcaaac ggacaccgat gatcgaaatg gatgcggcag acttccgcca agtaatcgac
    25021 gtggatttaa atgccccatt catcgtttca aaagcagtta ttccggatat gatcgaaaaa
    25081 ggcggcggta aaatcatcaa tatctgctcc atgatgagtg aattaggacg cgaaacggtc
    25141 agtgcttatg ctgcggcaaa aggcggatta aaaatgctga cgaaaaacat cgcttctgaa
    25201 tacggtcaat acaatatcca atgtaatgga attggaccag gttatattgc tacaccgcaa
    25261 actgctccat tacgcgaaac acaagaaaac ggtgaacgtc atccattcga tcaatttatc
    25321 gttggacgta cgccagcagc acgctggggc gagccaatcg atttggctgg accatctgtc
    25381 ttcttggctt ctagcgcatc tgatttcgtt aacggccatg tcttgtatgt ggacggaggc
    25441 atacttgcct atatcggaaa acaaccttaa aagagcttta gtacactgtt tagtggagag
    25501 gttgtgaaat cagcggattg aacgaatacc gaaggacagc tgattaaaca tcatttattc
    25561 gaaaaaagag gctgggacag aagcatttaa ctccaagaaa taagaaggaa ttcacgaaaa
    25621 ttgcttttca aatttttgtg aatttcagct tatttccgaa ggagttgctt ttacttccgc
    25681 cgtttgtacg tgtttagagc gtgagacaaa agtgttcttt acttttgtcc cacgctcttt
    25741 tctgtgcgtc ttccatactt cactaagtcg atccctcttg cttcgcacca atccttcact
    25801 ggtccggaaa agatcagaga ttgttggtgt agggccgcgg tattcgttgc gccgaccata
    25861 gtataaagca gacgaagctc ttcttgccat tgcttcatca gtataatagt ttcttctacg
    25921 ccgtgattca tcagatgcgt cagtacggtt cctgaagtac ccacggcatt tgcgccgaga
    25981 caaagggctt taaatatgtc ataggcattg cggatacctc cggaggctag gatttccata
    26041 gaagtgtctg cttcatttgc ttcaagcaat gaagagactg tcgattgccc ccagtcagct
    26101 aaataactca gttcccgctt ggagcgacgg gcgttttcga tctgggtgaa gcttgttccg
    26161 cttcgaccgc tgatatcaac tgtatgaact cctaaagcag ctagatcatt gagcgtttct
    26221 cttgtcatgc caaaaccgac ttcttttacg atgaggggga cgtcaatggc tgtttgagtt
    26281 tcttgaataa gtactttcca attggtaaaa tcacggtctc cctcaggcat aaccaactct
    26341 tgaggtgcat tcaaatgaat ctgtaaggca tctgcgtgaa acaatcggat agcttctttg
    26401 gctctttcga cagaagtccc tgcaccgatg ttggcaatga ttttcccgtg aggatattct
    26461 tgtcgcataa tcgtataagt atcggctaaa gaagggactt tcaaggcagc gctgactgaa
    26521 ccagtggcaa tcatcaaatc tgcttctctt gcgacgattg caagatcttg gttgattttt
    26581 tttgtttttt cgcttccgcc agtcatcgca ttgatataaa atgggctgct tagtgtaaac
    26641 ccaactgcct gtgtacttag atcgacatca gaaactgcta tttgcggcaa agggttatgg
    26701 atgatccgta cgaagtcaaa ttcgtttttt tgtttatcat gaaaggcttt agctagtgaa
    26761 acatgttcgt cttttcgatt catcggtttt cctttctttg atcgctatag acatgtaacg
    26821 gcagtggagt gatttctgct ttttcccatg cactcattaa aggaagaatg ccagatttct
    26881 ggtcaacaat tacgattccg caatcgcccc cacctgcacc agaagatttt gcggctcctt
    26941 catactgttc agctaaatta cacaatttgt tcaaagcagg cgtttcgatg acgaccccag
    27001 tgattgcaga taaatcatgc agtaattgtc ggttttttcg aatcatctgt tggatcaacg
    27061 ttacattatt ttctttaaac cctttgatca tttcgttgac acattctgta ctatttttta
    27121 agaaaagctg ataagccacc attttatctt ctctcgaacg gtgaacttga tcgaccaaat
    27181 cagaagtaga ggcagggcta cccgtccaac caatcaataa acgtaaatct tcaggagcaa
    27241 tcaatggctc aatggataga cctggccaat ccagggcaag taactcactg atagaatgct
    27301 gagtttcttg ttcttggagc caaggatggt cgaaggttga gaaagcgatc cagccaccat
    27361 aacagctagc agcgatgtcg ccgcaggaac cattatcttg aactgctaaa ttggctagtg
    27421 ctgcaatctt gaaaatctcc aactgagata aattcaacgc gtaaaaaaca ttcaaggctt
    27481 tgaccgttgc aacggtcacg gcaccgctag aacccaatcc atatttccgg ccatttgaac
    27541 tgtctaactc acttgttact ttcagatcat aaaaggataa aagaatgttt ttttcttgtg
    27601 catacttttc agtcaagcga atagcagcaa ggatataatg aaaaggattt tcccgaatat
    27661 ctaaaactaa ttctccgttg cgtcttgtcc aacgtacagg catcccacta tattgtgcag
    27721 attggatact tccgactttt cgtgcagatt ctacagtgac tgtcacgaat tgatcgactg
    27781 cagcgataac tgctggatgg cctgtttcaa caactgcata ttctccggca atatagagtt
    27841 tgcctggtgc agatacttca atcataaaaa tctcccttta ttcaataatc gcaattcctg
    27901 gaccagcatg tgcagtaatt acttgttctt tgctaaataa tttggtaaat gtttcttgta
    27961 cctcagataa atggctgttt tccactaaaa ctttgacatt agggccagca tccatcgtaa
    28021 agtagcatgg aatgccttgc tttctcaatt gtcgcaccgc atccatcgct tttaagctgt
    28081 caggagacca gtaagtgaat ggaggctggg cagctaaagt cgttccatgc atttttagtc
    28141 cgtttctttc catcgtttct ccgagaagtt ggaaatcttt tgttttgatt gcttgtttca
    28201 attgatatag atccccttca acggaatcta accagccttg ataaaaactg gatgtttcga
    28261 ctgtccgacg catcccattt ctgctggaca cttctttttt ctggtcgttg atcaaaacga
    28321 aaaccattgc taaatcgtct tcgaaagaat cggaagggac tggcttagcg taagaactta
    28381 agtcgtcatg gcctttttcc cattcgacga aaccaccgaa aatacttcgg caagctgaac
    28441 cagacccgcg tcgagcaaat cttgaaagag agagatcatc taatccaagc ttaagagcag
    28501 tattgcaagc gcctgctaaa gcagctagcc cgctggcaga ggaggctaat ccagctgcgg
    28561 taggaacgaa attctgactg attactgaag cttttagtga gaggccggct tctttacgaa
    28621 aaaggtcaag aaactgactg actttttttg ttgctttttc gtcttgcaag gtgccatcta
    28681 gataaaattc atctaccata tagctatcag aaaagatgac ttctgtctct gtataaaatg
    28741 catccaatgt taacgaaagg ctgttgttca ttggaaggat gagttcttca tttttcttac
    28801 cccaatattt gattagagca atgttggtat atgcgcgtgc tttgccttta aacataggta
    28861 tgtactccta atccttgtat ccaagtttca gcagcacccg catcttcaag tgcttgactg
    28921 atttcttggg cttttgtttt tgtttgtgct aatgcaatca tacagccacc gcgtccaccg
    28981 ccagttaatt tggcacccaa tgccccggta tcttttgctg tttggatcaa tagattaaga
    29041 aaatcattgc tgattgtcag cttttccaga agtgattgtg attcatccat tgtctgtgcc
    29101 aacgtttctg gtgaattttc aatgattgct tgttttgctt gcttcgtcaa gtatcctaat
    29161 ttttggatca tttgtccagt ttcatgaggc tgggtttcaa agaggtgagc gacatctttg
    29221 acggcttctc ttgtttgtcc tttgattcct gtatcagcga caatcaaaaa agcatcgatg
    29281 ttcaaagaaa agtagtcgaa aggatgccct tttgtaaaat aaatcggctg caagctgcta
    29341 gtagcggctg catcgattcc actaggatta ccgtgggcga ttttttccga aagctggaca
    29401 ttttctaata gtatttcacg agacaaagga aatgctaagt aatcataaaa agcacgagtg
    29461 actgctgtgg cgactgcagc gcttgatccc attccacgtt cagcaggaat cgtgctttca
    29521 atggtcagtt gcaagttttt ttggatgtca tgctgttttt ttaagtgaat gaataatttt
    29581 ttgatatttt ttaatgcatg aggagcttcg gctaaatttc ctgaatagta ggaagaaacc
    29641 agttgatcgt ccattgcatc cagctcttca aggaatgcgg tgacttttgt tgcatagaaa
    29701 ggaaaggcaa tcgctggttc tccataaaca accgcgtgct cgcccatcaa tatgatcttt
    29761 ccgcttgatt ctccttggcc atagtttgcc ataatctaac tccttctcac gtaaaagtga
    29821 tttttaagca aaaaataaag cgcccaaaac gagttgcgct gcttcacgaa tttttttgtc
    29881 cactaagggt ctcaaaaaac attcctcctt tattttacta aaaccatctc agaagtaaaa
    29941 ctaagaatac gttttctaaa gaaaaaacac ttttttctta agaatttttt gacttttttt
    30001 cttctttgac tagaaaaaaa tacaattgac actactagat attgggggta tatttaaaaa
    30061 ggttggagtt aaacacaata tttagtgatg aagtgagctg gaggtaaatg agcgtgatcg
    30121 ttttagcagg tacaattggt gccggaaaat caagtttaac agaaatgatg gctgaacact
    30181 ttgatagtca ggcattttat gaatcgattg atgataatga agtcttgccc ttattttatg
    30241 caaaccctga acagtatgct tttttgctgc aaatttattt tttgaataaa cgttttgcaa
    30301 gcatcaaaca agcaatgaaa gatgacaata atgtattgga ccgttcgatt tatgaagatt
    30361 cactgctttt ccatctaaat gcagatctag gaagagcaac agaaacagaa gttcgtgtgt
    30421 atgatgaact gctggaaaac atgatggaag aacttcccta tgctgcccat aaaaaacatc
    30481 cggatttatt agttcatatt cgtgtatctt ttgatacgat gttggagcgt atcgaaaaac
    30541 gtggtcgttc gtatgaacaa ttgtcctttg atccaagtct atacgattat tacaaagaat
    30601 tgaacagacg ttacgatcaa tggtatgaag aatacaaaga aagtccaaaa atccaaatcg
    30661 atggagaccg ctataatttt gtggaagatc ctcaagcaaa agaagaagtc ttgaaaatga
    30721 tcgaagaaaa attagcagag attcgccaaa ccaaagtagc catttgattt acagttgctc
    30781 aaataaaggg taaaccttta tttgagcgac ttttttcttt atactggaaa caaatgtcag
    30841 gaggatagag tatgcgggaa aaacactatt ttaatcaaga ccgtaatttt tggatcaatg
    30901 tacagcaaaa cgatgtgcta gctgttaaaa acttaaaaga agaatatggc attagtgacg
    30961 agatgctgac ttattctcta gataaaaatg aacgggcgcg cgtagagtac gattcttttg
    31021 atgaagcgtt actgcttgta tcaaatgttc cgcaccagca aaaagtcgat aatcattatg
    31081 aaactagtcc tatcgctttt attttaaaag aagacggttt gtttacattt accacgccga
    31141 ataccgagta cgtgattcga ctaattcgat cgcttttgga acgaatgcca gacatgagtg
    31201 tatacagttt attgtttcgt acattgtttt tgatttctga ttcctttttt cctttaatcg
    31261 aggaagtgaa tagtgaacgt cagcggctta atctgaaatt aagagaaaaa acaacgaaca
    31321 agaatctgct gcaactttca gatttagaga tcggtttggt ttacttagtt acaggaacaa
    31381 aacaaaatgt agtactttta gaacaaatca aggcattggc tatttatcgc aagttgtcag
    31441 aaaaggaaaa agaagagttg gacgatgcgc tgatcgaagc acgacaagca gttgaaatga
    31501 cgaacttagc ttcgcagatt ttagaccagc tctctggcac ttataataat ttgctgaata
    31561 acaacttgaa tgacacgatg aaatttttga cggtctggtc gctgatcttg accgttccaa
    31621 ctattgtgac aggattcttc ggaatgaatc tccagttacc ctttacccat tccgttttcg
    31681 gatggggaat cgccttgatc atcagtttgg tcttatctat ttggatgctt attgcactgt
    31741 ggcggcggat tcgttgatct agtctttgat ctgcaaacca agggcttggg cgatcacaag
    31801 tgcttgctct tggttgacga ttagtcccgc caaagaatct tgagataaag cgattgctgt
    31861 gaagaaatta gtcgtaaagt ctaatccctt cagagaagtt cgaaaccagt tcgaaccggt
    31921 caactgattg tcagacaaaa aaaggttttt ccaagtgacc tcataaaatt cgctgtcttc
    31981 taattgacaa tgatcgaagg agacagcttt caagtttgta ctactaaagg aagccatcga
    32041 agcaatacaa tcagtgaaag tacagtctcg aagataactt tcggcaaaat tcgtacctgt
    32101 taatttacat tgatgaaaca cgacttggtg gaaactgcca ccaatccact ccagattgga
    32161 aaaatcacat ttttcaaaaa tgacattact gcattcaaaa tgacgtaaaa ctgttttttg
    32221 catcgtaagt ttttggatat gactctctct taataccata tttttggcat cgagatagct
    32281 ttggtcaccc ccgttgatca gaagaccaga gaagatctcc tcatcttcta aataaaactc
    32341 tccctttgta agaagaggaa gagaaggtgg aataggttcg atttttttca tgtgggttca
    32401 cctcgttttt ctttagtata gcaaagaagc gggaatcact gctagaaagt ctgctaatct
    32461 aatcattttc agaaaaggaa gacaaactgt atgaaaaaca ttgacaaaaa gattagtttt
    32521 tgctatacta tgatggttga gaaataaaag cagaagcacc cgcttctcgc cttagttgat
    32581 aatgtcactg ggctagatag ttttcactgg ttctattagg tgaagtacgc gcgggttctg
    32641 taagagaact cgttttttta ttgccgttta ttcggtaagc ttttctttct ctcataatat
    32701 ttggaggtga atgaccatag caaaggatat gatggttaac gacggcattc gtgcacgaga
    32761 gttacgattg atcggttcag atggtgagca attaggagtt aagacaaaag cagaagcatt
    32821 gaatattgca gaacaagcaa acttggatct tgtgttagtt gcccctggtg cgaaaccacc
    32881 agttgcgcga atcatggact acggtaaata ccgtttcgaa caacaaaaga aagaccgcga
    32941 agcgcgtaaa aaacaaaaag tgatcaatgt aaaagaagtt cgcttaagtc ctacgatcga
    33001 cttgaatgac ttcaatacaa aacttcgtaa tgcacgtaag ttcttggaaa agggcgataa
    33061 agtgaaagct tctatccgtt tcaaaggccg tgccattacc cataaagaaa ttggtcaaaa
    33121 agtcttgaat cgcttagctg aagaaactgc ggatattgca acagtggaac aaaaagcgaa
    33181 aatggacgga cgcagcatgt tcttgacgct ggcaccgaaa aacgacagta agtaatttga
    33241 atgactaact ggctgttgga cagccgatag tcggataacg aaagattttg aggaggaaat
    33301 atagtcatgc caaaacaaaa aacacaccgc ggatcagcaa aacgtttcaa acgtactggt
    33361 aaaggcggat tgaaacgctt ccgcgcgttt acaagtcacc gtttccacgg caaaacaaaa
    33421 aaacaacgcc gtcaattgcg taaagcaagc atggtttcta gtggcgactt caaacgtatt
    33481 cgccaacaac tagcaaaaat gaaataagaa gtacaaataa cagaatctga agatttattt
    33541 ttattaggag gaattataca tggcacgtgt taaaggtgga acagtaactc gtaaacgtcg
    33601 taaaagagtg ctaaaattag caaaaggtta ctacggttca aaacacactt tatttaaaac
    33661 agcaaaagaa caagtaatga attcatacaa ctacgcatat cgcgatcgtc gtcaaaagaa
    33721 acgcgacttc cgtaaattgt ggattgctcg tatcaacgca gcagctcgta tgaatggctt
    33781 gagctactca aaattaatgc acggtttgaa agttgctgaa atcgacatca accgtaaaat
    33841 gttagctgac ttggctgtta acgatgcagc agcatttact gcattggctg accaagcaaa
    33901 agaagctttg gctaaataag ctaaacaatt ataaagagga aagaagataa cgaaaagtta
    33961 tgttctttcc tcttttttta tttacaatcc tccatcaaat gaaacaggac aataacgtat
    34021 aagtctaaaa tagcataatt aaggtttttt aattgaaaac tgaacattta tggtcaccct
    34081 ttaatgaaag cgctaactac ataagttaga atgagtttga ctgatatatt agtttgctta
    34141 tcagaaaaac aaaatggacg gaggaataac atgagtgata ttattattgg tattttgtta
    34201 gttatctcat tctttttcat ggtctggtac tgtgtaaaag gctacaactt gatggtcggc
    34261 tttgccatca tggctactgt atggatggga ttagcgctag tcggaaacac gttttcacca
    34321 aacccggcga tggaagggca aggagtaatt gatgtgttga cgcatatcta tacgacgggg
    34381 ccagctgagt acgccaaatc gatcttggta aatgtcttct ttggtgcctt tttcgggcgt
    34441 gttctagtag atagcggaat cgcagctacc ctgattcgaa aggtagttga acttggaggg
    34501 gacaagccaa gaatcacgat gagcttacta tgtattgtaa ccgctgttat attcatgtcg
    34561 atgacaggaa tcggaccagt gatttctatt gcagttattg tgttgccaat cttgatgtca
    34621 ttaggtattt cagtaccggt tgcactgttc tcatttatgg gctcgattat ggctggaatc
    34681 tttgctaata tcgtcaattt caaacaatat caaacgattt atgctggttt caatccagcg
    34741 gctgaaagct atacttataa tgactatttc caaattggta tgattgggat gattgtaagc
    34801 ttagttgtgg tactaacggt cgctaatata tcaatgaaca aaaagaaacg ctatgcaatg
    34861 gcagcaaacg taccagctga aggtggcgac gcaccaatga tctcatggtt agccgtatta
    34921 ttaccagttt taggggtcgt tcttttagat cttccaatca tcttaggttt tattctagca
    34981 ggtatctggg cattgctgtt tactggaaaa ttgcgcggag gttataaaga aatctgtcgt
    35041 cagtttgcga aattatttac agatggcgca gtagatgttg caccaatggt tggtttcttg
    35101 atgaccttag cgatgttcaa taattcagct gcgtatgctt ctccttattt ttcagctatt
    35161 tttggagatt ggataccgca atcaccatta gtcttggcaa tcgtatttgc tatattgaca
    35221 ccactaggat ttttccgcgg tccaatgaat ctagtcggtt caggctcagc tattttagct
    35281 gtcgtattag ctgttaatcc aacgatgtcg ccagcctttt tgttcccgtt atttgcaatc
    35341 acaacgattg ctcctcagca tttagatatt acacaatctt gggttgcttg gggattaggt
    35401 tatacaaaag taacttctcg agaatatatg aagaaatcca tcccaactgg ttggatcatc
    35461 ggagcgatct tatgcctgat cacgttctta ttgtacggga acgcctagta aacaaaacgt
    35521 cgataaaaac gattagatga atggaggaaa agaaaatgaa acgttttgtg cgaatgggaa
    35581 ttgatgttgg cggtacacat acgaaagcag tcgcaatcga taatgcgacg cacgaaatca
    35641 tcggaaaatc ttcagtgaaa acaacccacg atgatgtccg tggagtagcc gcaggtgtcg
    35701 ttcagtcttt tcaaaattgt ttgcgggaaa ataatatcag tccagaagat gttgtgtttg
    35761 ttgcacattc taccacacaa gcgactaatg cactgattga aggagacgta gcaaaagtcg
    35821 gcgtaatcgg gatggcaaaa ggtggattag aaggtttttt agcaaaacga caaactagat
    35881 tgaacgatat cgatttaggg aataagaaaa aaatagagat tgtcaatgca ttcttacctg
    35941 tcaaacactt aaatgtagac cgtgtttcag aaacgatttc ttctttagaa agagaaagag
    36001 cggaggtctt ggtttcttct atggcatttg gagtagataa tggggaacca gaacgagttg
    36061 tttatgaagc agcatcggta aaatcgatcc ctacaacgat ggcttctgac atcacaaaat
    36121 tatatggatt gacaagaaga actcggacag cagcaatcaa tgcatctatt ttaccaaaaa
    36181 tgctagatac ggcaacctct acagaagatt ccgtacgaga agcaggggtc aatgtttctt
    36241 tgatgattat gcgcggagac ggcggagtaa tggaaatcaa tgaaatgaaa aaacgacctg
    36301 ttttaaccat gctttccggt ccagcagcat cagtcatggg ctctttgatg tacttgagag
    36361 cgtctaatgg tgtttatttt gaagtaggcg gaacaacaac caatatcggg gtcattaaaa
    36421 atggacgtcc agcaatcgat tattcaattg taggtggcca cccaacatat atctcttcat
    36481 tagatgttcg ggttctaggt gtggcaggcg gttcgatggt acgggctaat cagtcaggaa
    36541 tcatcgatgt cggtccacgt tctgcacata ttgccggtct agattatgca gtatttacgg
    36601 agacagaaaa aatcaaaggt cctaaggttg aatttttctc tcctaaagaa ggagaccctg
    36661 ctgattacgt caaggtagtg atggaagacg gagaagaagt gactattaca aatacatgtg
    36721 cagccaatgt ccttggatta gtgcaagaag aacatttttc ttatggaaat gtcccatctg
    36781 cacgaaaagc catccaagca ttagctgatt attgtcatac gacggttgaa gacatcgcag
    36841 aacagatcat ggaaaaatcc tatgctaaga ttgaaccggt gattttagaa ttggcagaca
    36901 agtatcactt ggagaaagat cagatttctc ttgtaggtgt agggggcggc gctgcctcat
    36961 tgattactta ctttagtaat aaaatgggtg tgaaatattc gattcctgaa aacgccgaag
    37021 tgatttcttc tattggtgta gcacttgcaa tggtccgaga tgttgtagaa cgtatcatcc
    37081 cttcgccaag taaagaagat atacgatcgt tgaaaaatga agcgatgaat aaagcgattg
    37141 aatcaggcgc tacaccagaa tcgatcgaag tccacgtaga aatcgatcca cagacttcaa
    37201 aagtaacggc tatcgctact ggttctacgg aagtgaaagc aaccgatcta accaaggaga
    37261 tcacaacaga agaagcacta gagcttgctg cagaggatat gcgattaaat aaaaatgaag
    37321 tttgcttatt ggaaaataca cctttctttt atgtatgcgg cgaacagaat cgttcaaaaa
    37381 atgctggaag tcttcggatc atcgatcaaa aaggatttat caaggtccaa cgtggacatg
    37441 cttcatgtat gaaaacaact gcagccaatt atatgacagc tgtcgaacaa ctttgggaag
    37501 atatggcagt ttatcaaact gaattgattg cccgaccaga attttatctc tgcttaggag
    37561 caagagtctc tgattttaca gcgacggatc tggaacaact acagcttttg atggatctgg
    37621 aagtatctac tatggaaccg gaggaagaag tgatcgtcgt tgccggaaat atcaaacaaa
    37681 cataagttag aagagttact aaacgaatta caacagatcc ccgaagaaat ttggggattc
    37741 taccagtttc ggcgagattt attttggaaa aagattcctt tgtccaagca gaaaattctt
    37801 atacaacaat caatcgattg tggtatcgaa acggcatgta gcatcaaaaa aaagtaccct
    37861 tttgccgatg taggagaaat ttgtgaacaa atggctgttc caatcgtttc ctgtgaatca
    37921 gagcaaataa acgaacgaat cacttttgca acttatgcag aagatgaagg gattcgattg
    37981 atgacggagc cgttagaaaa attaaaatgt tcaggactag cttcgatctc taaagaaaca
    38041 gcgcaagctt tgattattgg acatgaacta tttcaccaca tcgaagcttc tgttaaggga
    38101 atttatacgc aaagcgaaaa aattgtgctg tggcggttgc ctttttatac acatcaatca
    38161 aacattcgtg cattgagcga gatagcagcg atgagttttt caaaagagat gaatcaaagc
    38221 gatttttcgc cttatgtatt agaggcagtc ttactttggc catataatga aattcgtagc
    38281 caagggatct tagaagaaat aaaaaaaata gaaaaaagat gtacggagta tgatttagat
    38341 tacaaataaa gagactgtga caaaagtcgt gtcacagtcc tttttttccg aataaacgat
    38401 gttctcctgt caactcctcg gtcacaagtc aaaatattca acaaacatga gaagctgttt
    38461 tttgaaattg ttccttgtga ctcggagatg aacggcaggc tcacgacctc ttggaaaacg
    38521 gtgttcaaaa gcttgcatct gcggtaataa gtccagccaa gttaaaaaca taagaagcta
    38581 tttttacttg gctgttctta ttacttgatg cagggcagct ttttcacaac ctctcaataa
    38641 acggtgtgca atcagctgac cctcggtatt agctcaaaaa aaacggcaaa atatgaggaa
    38701 ctattttgcc gtttctttct ctaatactca ggtcaaaacg ctgattccaa aacctcttat
    38761 ttgatgggtc tgtcctgaat atcttcaaaa taatcggggt aattatgaat cacgtattct
    38821 tgctcctcga taatcatatg taaatgcatc gtgcaaagaa atccgacttc atcaaagtta
    38881 tgatcaatca ttgattgaag cattgtctga tgttcatcta atactcgacc ccagtctaat
    38941 tctgaaatcg tcaaacgtaa ccatctgaaa cggttcaagt gcgtactata actttctagc
    39001 caatcccaaa tttctcgttt tccagcaatt tcataacaag ttcggtgaaa atcagtatcc
    39061 aaatcaaaaa aagctttttt attttgagcg attactgcct gatcggtttt tccaactttt
    39121 cccgcgtata acgggctaat gaagcagatc ggatatcgat ttttgaaaca taagtgccgg
    39181 actgtggaat ggtttttaca agctcttgat tgcgcagttg gatcagtgct tctcgaatcg
    39241 gtgtacgccc gatagctagt tgttcttcta acactttatc ggagatttta cttccaggtg
    39301 ctaactcgga ataaatgatt tgtttacgta tagattcata agcttgctgt tgcaaattat
    39361 tgataaccat ttaaccttcc tttctatgca gatataatct attataacat aaatcgctta
    39421 caaatcgtgt gggcataaaa acacagtctg caaaaaaagc cctgataggc atgcctatta
    39481 gagcctcgaa aagaaaaata ttgaatcaat ctggtcgtac ctcttgtatc ggtttgacac
    39541 gaccgccgat tcttttagca aaagcttcgg attctttttt gtctttgaaa atgagcagat
    39601 tgtctaagtt tttttcccga gtgcgacagc cagttttatc gatgtaaccg tgcattactt
    39661 taacgacata catggggatc acctgctttt ttcttttcat tatacgaact actcgacaca
    39721 atacaagaaa aatgcttaga cggtctttat taagaattta catcatccaa agaagcgtac
    39781 gcttggaata actgatctaa ctccggattg actttgctca gcttcactaa tcgttgcgaa
    39841 tcagaaagta aaaaacagtg tttactactg ccagtagttt ttaatttttt gtcttcagtt
    39901 cccacaattt cgtagctaaa atccaaccgt gtccccgtat acttttggat agttggatgg
    39961 atgctcactt tttctccgta atggatcatt tctttatagc tgcaatttac ttccagaacg
    40021 gggatgatga tcccagattc ttcgattttt ttatagggaa accctagatg taccaataag
    40081 gctacccgag cctcttcaaa ccagcggata taattgctgt gatgaataat ccccatttga
    40141 tctgtttcat aataaaaagg ttctcgcaca taacctgtaa atttctccat gactcatgcc
    40201 tcgttttcca ttgatttttt acaaaaataa ttcatccatt tcggaagtgc ttcgctgatt
    40261 tgtgtcggca aaacaatata ttgttctttt gccagctggt ctccaatgaa actatgaaga
    40321 taaatagctg ctgcaatcgt ttggtcattt tgaggaaact gggctaaaaa tcctgtgatc
    40381 atcccagcta aagtgtcacc catgccgccc gttgccatag cagcagaacc taaaggattg
    40441 gtgtagaagt gctggtgacc ataaattttt gtctcatgac ttttcaaaac gagcacagca
    40501 ccgatttcct gttgcttctt tttgttttct ttatcccgtt gttcttgtat cggtagagaa
    40561 ctcaaccgtt gccactccat ttgatgaggt gtaagtacag tttgttcggg atagggtagc
    40621 aaaagatgat tagcagcaag taaacttaaa gcagagccat caatcaccag ccattgttct
    40681 tttgattggt tttctagtac ttgtttgaaa cgttccaaac tcttagaaga agtacccaat
    40741 ccgggaccaa tcaagagtac atctgcttgc tttattacgg aaagaaactg gtcagtatcc
    40801 gtccaaacta caatcattgc ttctggcaga tgactgtgaa gagcactccg atttttttca
    40861 tcagttacta cagtcgtgag acccgctccg ctgtgtacgg cagctaaagc actcataatg
    40921 attgctccgc catattgttc attgccgcca atcaaaacga tacgtccaaa tgttccttta
    40981 tgtgaaattt ttggacgagg tgtgatgact cttttcacta gttgttcgtt tatcagcatc
    41041 taaaaacctc cttttactca ttatagcgta tatttttttt agagaaaaac agataagaaa
    41101 aggaattgtc agattttttg aactattttt ttctgtaatt tgtggtatca tgaacacgaa
    41161 gacttaaata agtggaggat taactttatg acaattgatt ggcaaaaaga agtagaagca
    41221 cgtaaagaag acttgcttga agacttgaaa aatcttttac gtgtcaacag cgagcgcgat
    41281 gactcaaaag taacaccaga tgcaccattt ggacctggac ctcgcgacgc attgaaacat
    41341 atgttagctt atggcgaacg tgatggattt aaagtaaaaa atgtcgataa ctatgcaggg
    41401 catattgatc ttggcgaagg agacgaaacg ctagggatat ttggacacat ggatgtcgtt
    41461 cctgcaggag atggctggga cacagatcct tatgaaccag taatcaagga tggaaagatc
    41521 tttgcacgcg gatcaagcga tgacaaaggt ccaagtatgg cagcttacta tgcgatgaag
    41581 atcattaaag aacttgactt gaaactatca aaaaaagttc gcttcgtagt cggcagcgat
    41641 gaagaaagcg gctggggcga tatggcttat tatttcgaac atgaagaaga accagatttt
    41701 ggtttctcac cagatgctga attcccaatc atcaatggtg aaaaaggaaa tgtttcttta
    41761 gctttacgct tcaaaggcga caatgcagga gattatgtct tgaaatcttt tgtttccgga
    41821 cttcgcgaaa atatggtgcc aggaacagca actgcagcac ttcaagtacc aagtgcagat
    41881 gcagctatcg cgatggaaga agcattttac caattcatcg aagcaaatcc agtaagcgga
    41941 acgattgaag cggacaatac gtatgtaaaa atcgaattag ttggtaaagg cgcacatggt
    42001 gcaagcccac aatctggtat caatgcaggt tctttcttgg cattgttctt agataactat
    42061 gaattcctag gatctgctaa acaatttatc catgtagcag ctgcttatgt ccatgaagat
    42121 ttttatggag aaaaactagg agtcgcttat gaagatgaaa aaatgggcaa attgacaatg
    42181 aatgctggac tatttgcttt tgaagaaaat ggtacagagg aagcgaactt catcaatatg
    42241 aatttccgtt ttccaaaagg tgtaacagta gacggattgc aaagtgacat cgaacaaaca
    42301 gtggggcaag aaggcgcaac agttactcga ggtgctcgcg taatggaacc acattacgta
    42361 ccaatggatg atccattggt ggctacatta ttgcaagtct atgaagacca cactggagaa
    42421 aaaggctacg aacaaatcat cggtggcgga acttacggac gtttgttgaa acgtggcgtt
    42481 gcttatggag cgatgttccc aggatatact gatacaatgc atcaagcaaa cgaatttatg
    42541 agtcttgatg acttgttcag agcaacagca atctatgcag atgccatcta tcgtttggcg
    42601 aaataattaa ttaaaaagag gatcaagaat tgcaaattat ttttgcagtc ttgatccttt
    42661 ttttactttc tactgtggta ttcatgataa acatgatata gattcaattc atattctttc
    42721 ttagcgttcg tgacgactgt atccaaaatc aaccctgtta ctaatgaaag aagtgctaaa
    42781 gtcatcaatc cagtacttaa aatcgcagaa gggatgcgtg taataaagct tgtcagcata
    42841 tattcgcgaa tcacaggaat ccccgtgacc aatccaaata aaaagaagaa gatggtccaa
    42901 atcccaaaga aaagcaaagg cttataatct ttaaacattt taataatcat catgatcact
    42961 ttgaatccgt cagagaatgt attcaatttt gattcgctgc cttcaggacg atcacgataa
    43021 tcgattggga tttcaacgaa cttgaatttt ttatctaaag catggatcgt caattccgtt
    43081 tcgatttgaa aaccagaact cataatagga aaacttttga caaagatacg attgaatcct
    43141 cgatagccgg tcatgacatc aagaattttt gttttataca ttctagtaat tgtatttcta
    43201 acaaggttat ttccaaaatc gtggaaaggt ctcttattct catcaaagta ggttccattt
    43261 gataggcgat caccaatgac catatctgct tcaccagaac gtaatttctc gagcaaacca
    43321 tgtacagctt ctgcaggata agtatcatcg ccatctacca ttaaataata gtcggcatcg
    43381 atatcaaaga acatttggcg gatcacattt ccttttcctt gacgaggttc tttttttacg
    43441 attgctccac cttgaacggc caattcgtat gttcgatcgg tcgaattatt atcatagaca
    43501 taaatatctg cttctggtaa ttctcgttta aagtctgcaa tgactgttga aattgttgct
    43561 tcctcattgt aacaaggaat cagaacagca attttttctt gttgttccat ttttcactct
    43621 ccttaactat agaaaacaaa agctaaatta aaaatacctc ataatcaaag gaaatgctag
    43681 tttttcccgc taaatatcat ttatttaaaa agaaaatact ggaatatatg tttggaaagc
    43741 tctatctaaa aaacca
//
LOCUS       DANKKG010000009        41129 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000009 DANKKG010000000
VERSION     DANKKG010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.009"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(69..1490)
                     /locus_tag="PW004_000422"
     CDS             complement(69..1490)
                     /locus_tag="PW004_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2725419.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(1733..3610)
                     /gene="tdc"
                     /locus_tag="PW004_000423"
     CDS             complement(1733..3610)
                     /gene="tdc"
                     /locus_tag="PW004_000423"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HDL2725420.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(3901..5157)
                     /locus_tag="PW004_000424"
     CDS             complement(3901..5157)
                     /locus_tag="PW004_000424"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HDL2725421.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(5269..5474)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5737..7038
                     /locus_tag="PW004_000425"
     CDS             5737..7038
                     /locus_tag="PW004_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDL2725422.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(7107..7706)
                     /locus_tag="PW004_000426"
     CDS             complement(7107..7706)
                     /locus_tag="PW004_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2725423.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            7961..8842
                     /locus_tag="PW004_000427"
     CDS             7961..8842
                     /locus_tag="PW004_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2725424.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            8855..10465
                     /locus_tag="PW004_000428"
     CDS             8855..10465
                     /locus_tag="PW004_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HDL2725425.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(10542..11201)
                     /locus_tag="PW004_000429"
     CDS             complement(10542..11201)
                     /locus_tag="PW004_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDL2725426.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(11484..12149)
                     /locus_tag="PW004_000430"
     CDS             complement(11484..12149)
                     /locus_tag="PW004_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HDL2725427.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(12296..12901)
                     /gene="dhaL"
                     /locus_tag="PW004_000431"
     CDS             complement(12296..12901)
                     /gene="dhaL"
                     /locus_tag="PW004_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HDL2725428.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(12929..13939)
                     /gene="dhaK"
                     /locus_tag="PW004_000432"
     CDS             complement(12929..13939)
                     /gene="dhaK"
                     /locus_tag="PW004_000432"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HDL2725429.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(13914..14360)
                     /gene="dhaM"
                     /locus_tag="PW004_000433"
     CDS             complement(13914..14360)
                     /gene="dhaM"
                     /locus_tag="PW004_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HDL2725430.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            14463..14783
                     /locus_tag="PW004_000434"
     CDS             14463..14783
                     /locus_tag="PW004_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HDL2725431.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(14895..15764)
                     /locus_tag="PW004_000435"
     CDS             complement(14895..15764)
                     /locus_tag="PW004_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL2725432.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(15860..17164)
                     /locus_tag="PW004_000436"
     CDS             complement(15860..17164)
                     /locus_tag="PW004_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL2725433.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(17161..17670)
                     /locus_tag="PW004_000437"
     CDS             complement(17161..17670)
                     /locus_tag="PW004_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725434.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(17845..19980)
                     /locus_tag="PW004_000438"
     CDS             complement(17845..19980)
                     /locus_tag="PW004_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2725435.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(20063..21718)
                     /locus_tag="PW004_000439"
     CDS             complement(20063..21718)
                     /locus_tag="PW004_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL2725436.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(21720..22034)
                     /locus_tag="PW004_000440"
     CDS             complement(21720..22034)
                     /locus_tag="PW004_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDL2725437.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            22204..22464
                     /locus_tag="PW004_000441"
     CDS             22204..22464
                     /locus_tag="PW004_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HDL2725438.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            22478..22792
                     /locus_tag="PW004_000442"
     CDS             22478..22792
                     /locus_tag="PW004_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HDL2725439.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(23522..24076)
                     /locus_tag="PW004_000443"
     CDS             complement(23522..24076)
                     /locus_tag="PW004_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HDL2725440.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(24202..26131)
                     /locus_tag="PW004_000444"
                     /pseudo
     CDS             complement(24202..26131)
                     /locus_tag="PW004_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(26437..27153)
                     /locus_tag="PW004_000445"
     CDS             complement(26437..27153)
                     /locus_tag="PW004_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HDL2725441.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(27154..28977)
                     /gene="glpO"
                     /locus_tag="PW004_000446"
     CDS             complement(27154..28977)
                     /gene="glpO"
                     /locus_tag="PW004_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HDL2725442.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(28991..30487)
                     /gene="glpK"
                     /locus_tag="PW004_000447"
     CDS             complement(28991..30487)
                     /gene="glpK"
                     /locus_tag="PW004_000447"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDL2725443.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(30623..32083)
                     /locus_tag="PW004_000448"
     CDS             complement(30623..32083)
                     /locus_tag="PW004_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2725444.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(32073..33368)
                     /locus_tag="PW004_000449"
     CDS             complement(32073..33368)
                     /locus_tag="PW004_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL2725445.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(33700..34377)
                     /locus_tag="PW004_000450"
     CDS             complement(33700..34377)
                     /locus_tag="PW004_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HDL2725446.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(34407..35498)
                     /locus_tag="PW004_000451"
     CDS             complement(34407..35498)
                     /locus_tag="PW004_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HDL2725447.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(35599..36033)
                     /locus_tag="PW004_000452"
     CDS             complement(35599..36033)
                     /locus_tag="PW004_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL2725448.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(36617..38074)
                     /gene="ascB"
                     /locus_tag="PW004_000453"
     CDS             complement(36617..38074)
                     /gene="ascB"
                     /locus_tag="PW004_000453"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDL2725449.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(38071..39966)
                     /locus_tag="PW004_000454"
     CDS             complement(38071..39966)
                     /locus_tag="PW004_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2725450.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(40024..>41129)
                     /locus_tag="PW004_000455"
     CDS             complement(40024..>41129)
                     /locus_tag="PW004_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL2725451.1"
                     /translation="SRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFS
                     NQEKQAIENRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLT
                     HIKQMKTRMDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYL
                     AEALNPFRKQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKIT
                     EYDAILTTEQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYY
                     KEENQIKIHKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLI
                     HQQRKIKRVFFVSFDYKRNDILVFFQAVSDLLG"
BASE COUNT        11603 a         8701 c         7157 g        13668 t
ORIGIN      
        1 aaaactattc acttggcagg acctcctact aaatcagcga caaaacgtcg gctgaaagac
       61 ttattttttt atttttcttc taaacttttt tctacctcaa tcccccaaga atctttcttg
      121 aagtgattga tgatcaatgg aataacgaac atgaccactg tgatagctgc tactaataat
      181 gcatcactca atgtcgctgt acctaatgta gcaaatacga caacgatgat agagaagatc
      241 agcatgcagc tgactaccca agctaatccg tttccgtttt taccaattcg gtaaggacgt
      301 tcgaattctg gatgtttctt acgcatttta agcaatgaaa cagcaatcaa aatgtacacg
      361 atacaataaa taacagttgt agcatttgta agtgtcaaga atacagcact tacgtcatcc
      421 attacaccat aaagcaaagc aaataatgaa ataacgattg attgtgttag aacgacattt
      481 ctagatacgc cgtatttgtt ttctttatgg aatccgaatt ttggtggaag gaatccttca
      541 cgagctactt ggatgattgt ttttgatggt ccagtaaccc aagctgataa ttgtaaaagc
      601 acaccaataa ataccatgaa gctgaagata tttccgatca cttctggtaa gcctaggatt
      661 tgacagtcaa tcaagatagg ctgcgtgata tttgctaatt ccatttttcc atctggtacg
      721 acatttgcta caagcatcgc attgatgaca ttcaataaaa ctaatccgat caaagcgata
      781 aatacgcctt ttgtataatt ttttgttgcg tctttcaaac gaggcatgta aacagaactc
      841 atttcgatcc caacaaaaat aaatgtgata ccagctaagt atttcaatga atcaatatgt
      901 tccaagtcag ggaaagcttt tgaccatgaa aagtcaccta gatagccgtt tgagactagt
      961 ccgactttga aggctgccaa aacacctaaa acgaacatga tgactactgg gatatatacc
     1021 cctagccata caccgatgtt cccgccgact tttgccatat cgaatttcaa gttcaagatc
     1081 gtgatgatcc agtagataac taaaatacaa cctaaaacga accagtgatt attgccaagt
     1141 tctacattac caaatgtgtt tcctaacaat ggtcctaaag tagaagcgac cattaccata
     1201 cctgggaaca tttgcaccca taatagccat gcaacgacaa atccccattt gctgcccagt
     1261 gctgttttta cccataactg aggaccgcct tcttgaggaa gcatcgtgga aagctcaccg
     1321 gaaaccattg atatcggacc ggcaaaaaac aaaactgcaa aaattgaata aaaaatcaat
     1381 gtccaaccga ctgcggctaa cgtaggaata ctccgaaccg ttgcacacag tgccattgtc
     1441 agaccgataa aggtcccaag acccatttta cttacttcag atttactcac cgttgacact
     1501 ctccttgatt ttttggataa aataacggtg tctttgaagt agtttcagca ataaacgaaa
     1561 caattcagcg aatatttgag gggattcttt gagtttattg cttgaagggt ttcttcaaag
     1621 acaacctcaa aacaaaggga tcgaagagct taggtctccc cttgctcttc agatccaaac
     1681 atgcgtcaga aacagttagt attcaagctg ttttctaaca tgattacctt gcttattttg
     1741 cttcgcttgc caataattgg tctgcgtaga ttttttccaa tttttcttga agagctgctt
     1801 tgattttttc agcatattct tcaaagtgtt cttctttgtt catgtatggt gtcattacgc
     1861 tggcacgcaa tacagttact tttccggcac ggttccattc ttcatctgag aatcctagtt
     1921 ggttcacaaa ttgtaatggg ctgttgccat agtctgggat agcaaagtca gtatgtgaag
     1981 ttaagaactc attaccgtaa atgcttcctt tcacgtatga agaataatca tatacgtcat
     2041 ggttcaattt attcattgca actaagtcat cgttgccttt ttctttgaat acatagtcta
     2101 ccatattgaa atctggataa gttaatggat gaacttcgat ttctttgtct cctactttaa
     2161 atgacaagtc attcaagaag ttgtagaaac gatgtgcacc ttcgattgat gcacccatta
     2221 atttaccata tcctgtgaca ttcaaaggta agacatggtg tgctgcccaa acgctggctg
     2281 ctgttgcccc tgcttttgaa ccttccaaga tataagcccc aagtaatgct gggatatctg
     2341 cgcctttttc aaatacatat gtcgcaaagt aagagataac gtctctcatg cggatatctt
     2401 tgatgacgat accaccggca gaatatggaa catagcccat tttatgtggg tcaatcgtta
     2461 cagattctgc ttcttcgatt gctttatatg cactgtgtac ttcttctaag atataatctt
     2521 tgttttctgt aaagacattg tatttgtaat gaacatcttt taaatcttca aatggaatga
     2581 agttgttgtc ttcgtctaag aaaatcgcac ggccgtatcc gccataagca gcatctacgt
     2641 gtaagtagaa gtaaataccg tctttttcta atacgcgacg caatgcaacg attttatcga
     2701 taccgtcgat tgctccttct tctgttgatc ctacaacacc gactacacct aggattggtg
     2761 ttttttcagc tgctagacca cgaacgattt tttcaagttc gttgatgtcc atgcgatagt
     2821 tgtggtcaac tggcactgga atcacttggt ctaaaccgat accgatgatg tcggcagctt
     2881 ttagccatga atagtgttta gtttgtggaa ccaaccattt tcctaatttt tctaaatgtt
     2941 ttccgctacg tgctgagtga gctttgattt cgtcgatttt ttctgggacg ctgtctaaca
     3001 gattcatgat ttcttctgtt gacaagttca ttaattccca atcgcttttt ccagcaacta
     3061 gttcaggtgt cacttctttc attgcaagcg gcaatgattt gatgttgcgg gcataccaaa
     3121 gtccttctaa gtttgctaaa gaaccatcag caacgatgtg gccccagcca tctttatagc
     3181 tcattaattt tgcaaattcc attcctactt cttcttccat ttggcttgtt gcaggagaag
     3241 attcataggc aacgttatta ccattccata gcatagcaaa attgtacgct agtaaagatg
     3301 gcatcaatgt ttcagagttc atatgacccc agtaacgtcc ggcattatgc caaggaacgg
     3361 aatgggtacg catccgcgct gaaatttctg atagaacatc ctttgtttta ttcacagtgt
     3421 gttcaaaact agcactgctt ttttcttctg gcgtaataat aggcatgtct tgaggcatgt
     3481 agttttgacg ccagcctaaa tgttcatcca ccaattcatt caacaaagct ttatagattt
     3541 ggccgttctc agctttgtcc ccaatgaata gggcatttag gtttagatct ttcgacaatg
     3601 attcactcat agtgaaaccc tcctaaaaaa atatttaaat tttgtcgagc cctacattac
     3661 acctgttctt ctattctgta aactaagttt tggcctgatt tttaatcata ttttaatatg
     3721 tatcaattat ccgaattgtt cacacttttg aaataattaa aaaaaacgag ggaatttaca
     3781 aatgtaaact ccctcgcaaa gattgtatta acgacaacca gcgttctatt ttaggatggg
     3841 tttcaaaatt ggaatgttgt aatagtattt ccctttttca aggtaaaaac ttactaaatt
     3901 ctaatcaatt actttggcca aaaagtaatt tttcttacct tttcgaataa ccacaaactt
     3961 accatcgaat tcatcggatg gatttatgac aaaatttaaa tcggtaacgc ggtcaccgtt
     4021 aatacttata gctccgtttg aaacatcttc acgtgcttga cgtttagatg gttcaatttt
     4081 tgtggaaaca agcagttcca cgatattttc aggtgtgctt gaaatttcaa cgttcggcat
     4141 tttaccgaat ccttgcgcga tttcttccgc atttagttct ttaatatttc cagagaacaa
     4201 tgcttgcgtt attttttgcg cttctttcaa gtcttcttca ctatgcacaa atcttgtaac
     4261 ttcttctgcc aaacggcgtt gtgcttcacg tttctcaggt tctgtctcta cttttttcgc
     4321 taagtcttcg atttcttctt gagacaagaa agtaaagaat ttcaagtatt tcactacatc
     4381 gcgatcatct tgattcaacc agaattggta gaactcaaat ggtgaagttt tcttcggatc
     4441 aagccaaacg gcaccgcctg cagtttttcc gaatttcgtt ccgtcagcct tcagcatcaa
     4501 tgggatcgtt aaaccgaacg cttctgcttc tggtccttct aattttcgga ttaggtctaa
     4561 tccagctgtg atatttcccc attggtctgc tccaccgatt tgcaattgca catcgtatgt
     4621 tttatgcaga tgcaaaaagt cgatagattg caggatttgg taagtaaact ctgtaaatga
     4681 gattcctact tccaaacggc tggcaacgat atcttttgct aacatcgtgt tgatgttgaa
     4741 gtttttaccg tagtctctca aaaattcaag taaagagatt tttgacaacc agtcgtagtt
     4801 gttcacaaat gtgatatttg catctttacc aaacaatttt ctcatttgat tggaaagtgc
     4861 gtccacgtta tgttgcactt gttccatcgt ttgcaacaca cgttctgttt tgcggccgct
     4921 tgggtcgccg atcgttcctg ttccgccgcc aatcagaata tatggatgat ggcctgccaa
     4981 ttggaatctt ttcatcatca taaatggaat caaatgtccg atatgcatcg aatcaccagt
     5041 tggatcaacg ccgcaataaa gtgaaatgct cttttcttca acgagctcgc gaagtctttc
     5101 ttcgtttgtt tgctgattaa tggcgtcgcg ccaagttaat tcatcaataa tattcatttt
     5161 actgacctcc gtgtagtttt tttatgaaag caggaacctg ctagcatgtg taattaatta
     5221 tttcttcagg ataggatagg aaataaaaaa agtcccttgg catataagcc aagggacgaa
     5281 atctcgcggt accacccaat ttgtatgtct agatatccaa catacctctc gaacatttaa
     5341 cgctgtcgcg tggcaatcac tgccaaagac tcctgaaatg taattcgctt taaagcatgt
     5401 actggtttgc agcgaccacc agctttctga aacattggct ttaacaacta cttcgtttcg
     5461 atcttcgtct tgttattatt aatatgtgtc tatcatagag cgcaactttt tctttgtcaa
     5521 cagaaaaagg atattttttt taaattcctc attttttctt ttttgaatcc gctctaattc
     5581 cgaacatttt attttcaatt attgttaaaa gaaataactt tttctgtatt ttttctgcaa
     5641 tttatcgaga agtcacttta ctttgcctct tattctttct tcgttacaat aaggaatggg
     5701 acaagtaaaa aaatgaaaaa gcaagggacg aggtatatgg aaaaaaagca aaaaattatt
     5761 ttaggagcca cttctgcttt aactatcgtt attttaattc taagtttgtt ttttgtcatc
     5821 cgatcatcta caaaaaaaga accaatcatt cctgaaaaaa aaataacaga aaaagcaaaa
     5881 gaaccgacag atgataagca acaactagtt actcaagcaa aaaatctggc tcaaagctac
     5941 gattatgacc aagcgattgc gttattggaa aaagatagta agaacggatc tactgaaaca
     6001 caacagcttc ttcaaacact aaaaaaggag aaagaacaac tgatcgactg gtctgatcct
     6061 actcagatct ctcatgtctt tttccacagc ttgatcgtgg acccgcaaaa agccttccac
     6121 tctcaacagg cacaaggata caaagattat atggtgactg ttgaagaatt caaccgttcg
     6181 atcgaacaac tttatcaaaa tgattttgtt ttagttaatt tgaatgattt gatccaaaaa
     6241 gacgaacaag gaaatctttc ctttaacggg cttgcgctac ctgaaggaaa aaaaccattg
     6301 gttctttcac aagatgatgt cagctattat gaatatatgg ataattccgg ttttcctagc
     6361 cgattggtcg tatcaaataa caatgaaatc aaaaatgtct ataaagagaa agataaagaa
     6421 gaaatcggtg actttgacat ggttcctctg atcgatgcct ttgtcaagaa acatcctgat
     6481 ttttcttatc gaggcgcaaa aggtaccttg gccctgacag gttacaacgg agttttaggt
     6541 tatcgaacat caaaatctca atatggagac aatgaaaaaa caaaaaaaga gattaaagaa
     6601 gctaaaaaag tggcggaaca gttgaagaaa gacggctgga gctttgcttc ccatacatgg
     6661 ggtcacttga atatgactca agcttcacta gaagatgtaa aaaaagacaa tgaattgtgg
     6721 cagcaagaag ttgcacctat cctaggaaaa acaaatattc tgatttatcc atttggtgca
     6781 gacatcagcg actggcagcc ttattcagca gaaaatcaaa aatttgctta cttgaagagt
     6841 caaggtttcg atattttctg taatgtcgat gcttctacac cagcttgggg acaattaggt
     6901 acggatttct atcgaaatgc tcggatcaat atcgatggca ttcgttttca atctgattta
     6961 gacggaaaaa atcctattct tgatccattc atcaatgtcc atgatgtcta tgacaaagaa
     7021 gcacgcgaat ctgcttgatt ttacgtcatt tatgaaccaa aaaagccgaa ctgacatttt
     7081 atcgtcagtt tcggcttttt tatgctttat ttcttcttcg gtgcgattcc cttgatcagg
     7141 acttctgtcc attctgctgt atatggaagc agttttgcgt atatctccgg cttagaaatt
     7201 ccttctacca ttgcttgaaa atagaggatc aagaactcat ccgaatattt caaatccact
     7261 tttccttctt tccgccctcg atggaaaaga tcaagcatga tactgaaact ttcttgcgta
     7321 tacgcatcca tcgcgtcaga aaattctttt ttttcttttt ttgtatagta tgtcatcaaa
     7381 tcgatataaa attgtttgtt cactcgattg agataacgga ttttgttttc actcatggca
     7441 atcagtgttt cttcaaatgg aagattttcc gcaatcaacg ctttcgcttc ttgccctaaa
     7501 tcattcataa atgcaaaaaa tacttcataa atcagccctt ccttgctgcc aaaatattta
     7561 aaaatcgtcg ctttacttac ctgagcgttc tcagctacct tatccattgt cactccagcg
     7621 gttcctatgt ctgtgttcat caagtcaaaa gctgcttgta aaatctcttt ctttttctct
     7681 tgtgctctct tttcaaaacc gttcatttgt agagcctccc gtaattttaa acttttataa
     7741 tatatatagt tcaaatttat tctgaggttt attataacta tctattgaga aagtatcaag
     7801 ctagcaattc cttgtttatg aattcattga cagaaattta taaaaagaat attatgtact
     7861 aaaataataa aaaaaagttt tataaaaagg tataaaacct ttttattaaa ctatttaatc
     7921 ttgtttagta tatattttta gaaaaaggag tggggttttt atggcagaaa tcgtcaaagt
     7981 cacacatttg aaaaagagct ttggtcgttt cgaagcttta aaagatgtct ctttcaccgt
     8041 acattcaggt gaagtcgttg gatttatcgg accaaatggt tctggaaaat ccacgacgat
     8101 tcggacactt ttagggatta taaaaaaaga cggcggaacg gcaacgattt ttgggcaaga
     8161 tgtatgggaa gatagtttat ctatccatga acgtctttct tatgttccag gcgatatctc
     8221 tttgtgggga aatttgactg gaggagcgat catcgatctc ttcatgaagc ttcatggaaa
     8281 agggagtgct caaaaaaaag atgaactgat tcaacgtttc tctcttgatc ctaagaaaaa
     8341 agcaaaaaac tattcaaaag gaaatcgcca aaaagtgggc ttgattgctg ctttggctgt
     8401 tgattcggat ctctatttat ttgatgagcc tacttctggt ttagatcctt tgatggaaac
     8461 gattttccaa gaagaagttg aaaaattgaa agaacagggc aaatccatcc tactttcttc
     8521 tcatattctc agtgaagtcg agcgactagc ggataaagtc gtgatcatac gcgaaggaac
     8581 actcgatgaa gaaggaacac tcgatgaatt gcgtcatttg actcgttcga ccatcactat
     8641 agaaacagaa gcagagaata gtcacttagc acaattgaac ggtgtccatg actttgtgca
     8701 aaaaggtcac ctcgctcaat ttgctgctga taacgaagct ttcgatgtga ttttagctga
     8761 agcgtccaaa ttaggtgtga aaaaatttga atctattcca ccgactcttg aggatttatt
     8821 catgagacat tatgaaggat gaggaggaag cgaaatgact gataaatttt cacgctggtc
     8881 cacccttttt tctcaaaacc ttagccgtga ctggaaaaag atcctgatat ggctagtcgg
     8941 tgtcactgct ttttctggtg gttttgtccc tgcgtttgaa gaattgactt ccggacaagg
     9001 attagctgga ttatacgaaa ccttgcaaaa cccggcaatg attgcaatgg tcggaccaac
     9061 tccagcttcg aatgctgctg agtatacttt aggagcgatg tatgcaaatg aaatgttgct
     9121 tttttgcagt ttgtttgctg ctgtgatctc tgttttacac gtggtgagcc aaacgagaaa
     9181 aaaagaagaa cttggtctga ttgaacttat tcgttccttt cgagtcggac gtcaagcaaa
     9241 ctctttagct gtcatcgctg aaagtattct gatcaatttg cttttagcaa tatcgatcag
     9301 ctgtacgatg atttttttcc aagcggattc gatcactgca gaaggtgctt ggctttttgg
     9361 gctatctgta ggtagtatgg ggattttggg gagtgccgtt gcactgtttg ctgcacaact
     9421 gatgccaagt tcagccagtt cgaatggtct gtctttagcg tttttaggtg gactttacct
     9481 tctacgagca ataacagatg tagctgattc ctccctttcg atgtggaatc ctttaggctg
     9541 gtcttatttg acctatccat ttacaactaa caactggatt cctttgcttt atttgttttt
     9601 gttcagtcta atcttgataa tcagttcatt tgttttagag gaacatcgag atttgaacac
     9661 cggctatatc cccgaacgtg aaggaaaacc gtatgctaaa cgttctcttt tatccttgcg
     9721 tggtttatta ttctatctaa ataaagggat gacaatcagt tggcttatca cttttggttt
     9781 gatgggggcg gcatacggct ctatctacgg gaatatgcaa cagtttttag aaagtaacga
     9841 cttaatgaaa cagatgttta tccaatctgg cgtctcaatt gaaaaaaact ttacaagcac
     9901 gatcatggta gtcttgattt gtctagctgc tattttacca attgctagca tcaatcgttt
     9961 atttacagaa gaaacccacg gacgtatgaa tcagctgttc gcaacaaaaa tttctaggaa
    10021 ccaactttat tggaccaata tgttactagc tattctattt gctatattgg caatcctgat
    10081 tagttctggt gcactagggc tttctgcttt ggcttcgatg aaggatgcga aagacatgga
    10141 tctattgatg tttcttaaag ccgggttcaa tttcctgcct gccgttttag tgtttatcgg
    10201 aattagtggc tttctattcg gatggcttcc tcgaatcggt aaacttgctt atatctactt
    10261 agcttactcc tttgcactca attactttgg cggaattttg gatttaccag aatggttttc
    10321 caaaacagct gtccctagct gggttccacg gatgccaaac gattcttttg atctttccgt
    10381 gttccttacg ttatcatgta tcagccttgc actgttcgtc cttggttcta ttggttatag
    10441 gcgaagagac ttactagaag gataataaaa aagcatgtac aatttcatgc tagcctccaa
    10501 atctgggtca aaaatccaac atttggaggt cattttttta tttacaagtc ttctaactgc
    10561 tctttatcaa tgatccagac ttctcgattc tttcttttta cataaccatt ctcctctaat
    10621 aatttaaatt ttctagataa agtttcgggg gtcgtaccga taaatgcagc aagttctttc
    10681 attttcattg gcaggaaaaa gtgatccttc cctgtcgctt tagctaagtc aagcagatag
    10741 acagcgactc gttcttctac tttttccatc atcaaaaaag ttgtctgctg ctgtactttt
    10801 gttaatttct caacgttgat tgctaaaagt ttcagactaa gggcgggata atgcagtaag
    10861 agttccgaga aatctttttg tctcaatatg caaatttccg ttttttctag tgcttcgcca
    10921 aataaagact catttggaac gcctagcaac tggctctctc cctcatatcc gcccggctct
    10981 attactcgta aaagctgttc ctttccgcta gcagataatt gatacacttt catactgccg
    11041 gaggccacga tcactaattg aggttctcca ttcggcgcaa gaatctgttc tcctttttcc
    11101 acatagtgat gtttgacaag tgcgtgtact ttttcttgat cttgctgtac taagtgattg
    11161 aacaaaggaa caaatgaaac acataaatga tcatgactca taatctctcc tccttctaaa
    11221 cagctatttc taataaagaa acataacttc ccttattcca actgataatt ttcttttatc
    11281 atagcataaa cgtcaagcga accagatacc agcaaataga gtggatttct ttttgtccct
    11341 tgctttattt tccttctttt gagaccttaa gtaattgatt cctatcaatt ttttaaatat
    11401 ttactttttg aataatcaaa aacaaacctt cactcgcaag ccgagagtaa ggtttgtttt
    11461 tcgcattgct tatttcgtgt tgtctattga aatttttccg cacctagctt ttgatatagt
    11521 ggtgctagtt cttgtaaggt ttcagcggtt atttgctcaa cgtgttcgat ttcttgcgga
    11581 tagaagaata gtttacccat ttgaaaggtt cggtctctca tcgctttttt caactcttct
    11641 tgtttctttg tatcggacaa atagtcagac aaagctaagt ggtcgtcaag ttcatgttct
    11701 ggttgcggcc atatgtaata atccttgata tcgactgttt tgctccactc tttcagtatc
    11761 gcaagcaact ggttatacgc tgcaatgcta ccatatcgtt cttcgctttt ataatgttgg
    11821 tacatcaaat agatttggta ttgtttttga ttcaacaaaa ctgctagaca ggcattctcg
    11881 tcttgacgag attcgcttcg gtacgcgctc caaaaatgac tgcgtaaatt ccagccattc
    11941 gtccagcttt cgattttagg tttgctcatg ccgtacatat caggcaaaag tgcagctgtc
    12001 tggagctgaa tctctttcca tttttgccag tgttctttat attctttctt gatttgttca
    12061 atcttatctg ttggatggct ctctttgagc tgtttgaatg cgtagattgg tcgatcaaac
    12121 aactgataat ctgattttcc aagttccatt gtttcgcctc tttccgttat cttttgtatt
    12181 ctacctgaat tttgcgatat ggacaaatag aggttgtgaa aaagctgttc tgcatcaagt
    12241 cataagaaca gccaaacaaa aaactgcgat gagcatcacc gcagttttat tgaacttaag
    12301 gttgattgat cacatctaaa agcgttgaaa ataaaatggc actagaagca gctcctgggt
    12361 cgatatgtcc gattgcacgc tctcctagat aggaagctcg tcctttttta gctaccaaga
    12421 ctttggtgtg ttcttttgcc tggtcaatga tttcttttgt taaattccct gccttcaatg
    12481 cctctgtgac tggcagccaa acatctaaca tcgttttatc gtcagcttct gcctttcctc
    12541 ggctttgaat acccgctgtt ccctgctcga tcacttcgcc taatttttct tgagaatcaa
    12601 tagaatcgac ctcttttgtc gctttcatca tgttcatgaa tgcagatccg tataacggac
    12661 cagaagcccc accgactttc gatatcatcg ccatagagaa tactttgaat gtatcgctga
    12721 ttgtttcagg atgatcggct tgaaaagctt cttcataagc tgaaacccct cttcccatgt
    12781 tatttccatg gtctccatcg ccaatcgggg tatctagatc actgagatat tgtttgttct
    12841 gattgattgc ctctgcaaat tgcgtgagcc attcttggat ttcttgaacg gttagtttca
    12901 tattatcacc cttttatctt gagatttttt accatgaaat cgtctgtaca ttgctttcta
    12961 aagaatcttt ccattgtgtc tccgtcaagt cgatcagtgt tagagaaaga ccttgcatat
    13021 ctagtgaggt cataaaatta ccgacttttc ggaaaactac attgacgcct ctttcttcca
    13081 acagagctaa aacatcattc atgaacacga actgttccat caaaggcgtc ccgcccattc
    13141 cgttgactag tacacctgct tcttttggta aattcgagta atcatctaaa atcttagtaa
    13201 caagttcctt tgccagtact ttagaaggct gcatcttttc tcgacgatag cctggctcac
    13261 cgtggatacc aacaccatac tcgatctcat cttctcctag ttcgaatcct ggtttcccta
    13321 cttctggtac agttgctgct cgaagcgcta gaccgatcgt cttagttgcc tgtacgacct
    13381 tttctcccag ttctttcaac tcagctaggg aagcaccgtt tcttgctgca tcgcccacga
    13441 ttttgtgtac aaggacagta cctgcaactc cgcgttttcc agcagtatac gtactatttt
    13501 caacggcgat atcatcatct acgatcacac tttctacttc aatatcgtcc atagcagcta
    13561 attcctttgc catatcgaag ttcaatgcat cccctgtata attcttaacg atcagcaaaa
    13621 ctccttttcc actatcggct tccttaatag ccgtctgaat ctgatctgga gttggagaag
    13681 taaacacatc accaagcacc gctgcactta acatcccttc ccctacaaac ccggcatggg
    13741 atggttcatg tccgcttcct ccaccagaaa ccaaaccaac ttgttccgct ttctgattct
    13801 tggctacaac acgggaagat tctacacgat gaacaagttc tggatagctt tttactaatc
    13861 cttccagcat ttcttcgaca atattgcttg gatcattcat gatttttttc aatttattcc
    13921 catcctttct ttccggcatt ttgtgcttct gttaagattt gctgagtatc atctgttata
    13981 ccggcagtga tggctgctgc aaaagctcct tccactaaag gcgcatccac cagatgataa
    14041 cgttgctgct gctcttcttc caacatgtct tttgccagtt ccgagttcaa taccgcgcta
    14101 ccgatatccg caaaaacaaa ataactagct gcatcgcttg attcattgac tgcttctacg
    14161 attttcatcg ggtctgatcc gatctcgcca tttattccgc cgagagaaaa aatttgcacc
    14221 tcttcggatt tttgcatctg ctcaatcatt tctttaatac catctgttac tttttgacta
    14281 tgagaaataa gcaaaatact ttttttcaca agaatcttcc tttcttttac atagttgcta
    14341 acgattggtt tcgctttcat taatgaacaa ttcaagtata taataaattt ctaattttgg
    14401 gaaaatatgt gtagtgcttc ttgaagtttg ttatacttta attaaaagag aggagtgatc
    14461 gaatgattac aacaacaacg aacagtgtag agggaaaagc cgtagtagct tataaaggga
    14521 tcgtttttgg tgaggtcatt actgggatcc atgcatttaa agacttggag gcggggttgc
    14581 gaaatatctt tggcggccgc tcaaaaagct atgaaaacga actaatgggt gcaagagaag
    14641 aagcacttgc cgaaatggcc gaacgtgcaa aagcattagg agctgatgcg atcattggcg
    14701 taaaaatgga ctatgaagta ttaggcagcg acaatggcat gctgatggtc acatgtagtg
    14761 gaacagctgt ctccacgaga taagtagggg acatctgttt taataactaa agagagatgg
    14821 gacatgtagt ggcactcaag aattagattt ttcagtctaa cttcgaggta ctctaccttc
    14881 ccatcctctc ttttttattt ctttttgcgg atgattccgt aagctgctgc gactaacaca
    14941 cccgcccctg ctaaggaata actgacagaa gctgtttttt catttgtttt tggcaaagtc
    15001 tgacttgttg atttggatgg ctctgtcgtt tgctgaactg tttctgtcgt gcttccagag
    15061 gtcactgatt tttctcctgc cgaatgcaaa atatctttta ttcgttggaa actagctgct
    15121 aagtcagctt tttttgcttc aaaatcatta gcaactgttt gtgcttcgct gatctttccg
    15181 tggattgctt gactggccgc tgtgatatcc gcttgagttt gacttagctt tccagaagct
    15241 gtttgagcaa cctgatttaa ttgctgattg aattcttttg cttgttggat cttcccgtca
    15301 agatctgttt ggacagcttc cgcttttgct ttcgcttcgg caagcttttt ctcaagttgt
    15361 gcacggtcat ttcccacttt atttttaaga tcagctaaat cttgatcgat tgctgcttgg
    15421 tcttctttca atttgttttt tgcagcttct aatgcttctt cgagttcttt tcttttattt
    15481 tccaagtctt gcaaatgatc gatttggtca gaagaggtag aactatttat tgatgtatct
    15541 tctgaagatg aaatcgtttc tcctgaatta tctgtcggta cattggaagt catttctgtt
    15601 tccgaggcac cgttttcagc accagaagag cttatggttg ttgaaggttc tgtttgagca
    15661 ggttcagaag tttccgcaaa agctgtctgt cccaataaga aaatactgga aacagcaatt
    15721 ggccagatta acggcttcat tctttttttc atcacttttc ccatccttac tactttttaa
    15781 ctaatttctt attataagca aaaatgtttg gaagaaccat aaacacaaca ggctgtttgt
    15841 ctaaaaaacg gtgcatcttt tatttttttg tacgttccgg gcttttccat gtcccgcttc
    15901 cataacctaa aatcgttttg actgctggaa tgaaagtcgt cacgatcgtt atcgcgttca
    15961 ctatccagaa caaaagcata tatagtggtg cgaaaagaag gtatttcaat tttcttcgtc
    16021 gatcatctac aatcagcgaa gctagcaact ggaaaaatcc tgcgaccatt tcaaagcaga
    16081 tgaacacaaa actgaatgta aaagtaatat aaatctgttc gtagtttcct tgatatacat
    16141 aatgcccgat taatccagca aaaagaacta cggaaataca aaagaatatt gaccagacaa
    16201 tactcaatgt ttgatcaatg aagatgatcg ttcttccaat atgtttaaaa ggatggagca
    16261 tcactttttt gaaattcgtc agccagactt ctgttccacc ttttgcccaa cgttttctgt
    16321 gtcgatatag catcttcaac gtcacaggta cttccatgaa aaagataatc tcaggagcaa
    16381 aaacagataa ccagtcattt aactgatgat cccaagcaat actgatgtct tctgtcgctc
    16441 gatcttgtct gaatccgccc acatcgatca aggcttcttt tcgatacatc gtatttgctc
    16501 cactgtacgc ataaagacca ccgaaaacag ctgactgtgt tcgtttaatg atgcccacga
    16561 tacttgagaa ttcaaccgtt tgagattttg caatcaattt ggtacgattt tggacatcca
    16621 tatttgcagt gaccgcagca atatgtcttg caccagggcg aatgaaataa ttcacatacc
    16681 taatcagtgc atctggctcc ggtaccgtgt ctgcatcatt gcttaaaatc aatttccctt
    16741 ttgcaaaggc aagtccgata ttaaacgcgt gggcttttcc tttatttttt tcgatccgaa
    16801 ccacacgaag attagcgtat tttttcatca gtctagctaa tatttctggt gtttggtccg
    16861 ttgaaccatc gtctgtcact aatacctcat agttgtgata attcaatttc gtcatcaaat
    16921 actcgattgt atattcgatc acaatttctt cattatgggc tggcaccatg atcgtgatga
    16981 acggttcgac actcaaggga acatctaccc attctttttg cttatgtttg tataaaaaca
    17041 cataacacca gacaccaata aaccatacaa atccgcctaa aatcggataa gaaacgagaa
    17101 aaaaatacat aatagacaaa caagtatcta agaataaatt acctgtatgg gtgattgaga
    17161 tcatttcagt tccaccccat tttctttata caaatctgca atgaatgtcg tatctagatt
    17221 tttctcttcg gagacagaat aaaaacggac cgtttctctc tcttcttttg ttccaaaacg
    17281 ttccgtatac acttcttcaa gtaattcttg tctttttttc aatttttcat cttcatattc
    17341 aaaagattta tttacttttt tgtatagatt tcgattgttc cacctagtca atacaaatga
    17401 ggtaatcagg aaactgaaaa aaatcaatac aaaaaagccg attaagaaat acagcatttt
    17461 cttttctgat aaaaaagcgt acaaggaaaa atccttggct ctttctggtc tcgttaatgg
    17521 gaaaaagacc catataaaag gaagaacaac gatcatccat cctaaaagag ccactaacgt
    17581 ttgccaaatc tttaaccagt aattaccttt tgaaaaatac ttatctttca ccaaaattga
    17641 tgcaacttct tctcttgtta cttttttcat atcctcacct cacgtttttt tgttacgacg
    17701 taggagttta ccatacattt catttctttt cacataaagt gttcctaaat ttttcctaag
    17761 aaaaagaaca aaacatttct catcagcaaa tgaaaaatat tttgttcttt ttatcagttg
    17821 ttctttttat tttttatctc ttaattatgt tgctctttcc aatacgacaa tgcttttcat
    17881 tcctctagat atcgccgtgt acatttgtct gcgtcttctt tgctcatctc catcgttttc
    17941 aaatggatat aagattacat gatcaaattc cagtcctttt gctagactca aaggaatgat
    18001 ttggcagttt tctaatccat atgaattttt tttaatcgat tcttgtagcg tttgggtttc
    18061 ctcttctgtt tgggtgataa ctgccgcttt ttctcctttt agagaaacca aaatctcagc
    18121 tagtattttc aacaattctt cagaggatcg aaaagagcgg tattcgggtt cttttccttg
    18181 cggtcggatc gatacgatgt cgatcttctc ttgatccaca gcataggttt tgaaaagctc
    18241 tgtgattgct ccgctagaac ggtaactatt tctaagatca taccgatgga tcggcagatg
    18301 gtagtcgtcg aaacaacgca taatatcagc aaaagtagtg gaagaattaa agattgcctg
    18361 attctcgtca cctactaaag taaagtgagt ttttggaaat aattcgatca atagacctaa
    18421 ttgtgccagg gtataatctt gtacttcatc aatcaataga tagcgaaatg ctggtacatt
    18481 taccttttca acaagtaaat gacgaatagc taatataatg actgcttcat caacagtacg
    18541 tgtgggttgc caagcataag ctgagccata tcgtttttca taaattcttt caaacaactg
    18601 atgctcatcc acccatgcca tttcttcgat ttgtctggtt atttttcgat attttttttg
    18661 taaaagtttc cttgcataag ctacaatact ttgttccgaa tcatcagaaa tcaatttacc
    18721 gaataattcc aactgcctat cttctgtcaa cgagagtacc tgattctgca cagcaggact
    18781 ttttgcttgt ttcaaaagat gattcttcca ttcgctcatc agtgcctttt tcgttgcttg
    18841 aagcctatcg atcaatttag gatgagcagg aactttctca tatatttttt cgatcgtttt
    18901 tttagaaagg actgtttttc catttttagt aagtccacgg ataaaattcg gatgatctaa
    18961 aaacaaagga tctgattgtt ttagattatc gataaacttt ttgctacgca aacgttctgt
    19021 ctgctccgat acattttctc ccgtgatacg tttgaaataa gcctcttcac cttctatttc
    19081 ctctgctgaa agctgtgaaa ccagctgtat gatggttaga ttcagtggat ttctttcacc
    19141 taatgatggt aggacattcg aaatatagtc gataaaccga tgattaggtg atagaatcag
    19201 caagtcatcg gatgtcatcg tatctcgata taaatagagc agatacgcaa tacgctgcat
    19261 gatagcagag gttttaccac ttcctgctac gccatttacc aagatatttt gacttttcac
    19321 atcccgaatg attgtgtttt gttccgattg gatcgtagca gtgatagcct tcatttcatt
    19381 ggtctcatct tggcctaaga cttctaataa tacgtcatct tgaatagcta cagacgtatc
    19441 aaaaaacttg accaaacgat ctctttccaa aagaaattgt cgtctttctt taataaccgt
    19501 atcgatttta ttttgacgaa ccgtataaga agatggacca atcatgttat tgtaaaaaag
    19561 ttcggcaatt ggtgaacgcc aatcataaac aagattttca tctgcatcat ctgtgaaatt
    19621 tgctgtaccg atataaaagg attcttttgg ctcattatcc agaaagtcta caacgacttt
    19681 tccaaagtag ggacttttca ataaacgttc gatctttgct agctgatttt ctgcgttttt
    19741 ctgtttgata ttcatctgat cgatttctct atttttcatt tcaatcatcg aaaaagtatc
    19801 caggttatct aagtaactgt caaaattcaa tcgaagctcg ccagccattt ctttcaacga
    19861 ctggtttcct tctatttttg ccgtatccat cgattcccgc agttcgtttt ttttatcaat
    19921 tagcttttga tacacgttct tcagatatgc ttcttctaac tgtttttgtg tgttgaccat
    19981 acacattctc ctcttttttt aaagtggtgg aaccgattag tttacctctt ttaagagtcg
    20041 aatgcaagct ttttaacgag tatcatttgt taggatatat cagttcttca ggtcggacag
    20101 tttgatataa agaaaatgca ccatcaaggt tttttacgtg gtagccagac tgcttcaaga
    20161 ttctttccgc tagatagcta cgtaatccgc tatgacagct gacgatatat tcttgttggc
    20221 tgtctaattc atttaatcgg tcccgaagtt cattcaaagg gatatgtttg gcttccttga
    20281 atttgccctt ttccaattct tctgctgttc gcacatctaa taaaattttt ccttttgcta
    20341 gttctgtcgg caattcatgc cattgtacgg tttcactaag cccttctgca atgttcattg
    20401 ctgcatatcc tagcatgttt actggatctt tggctgagcc aaatggcggt gcatacgtca
    20461 attctaattc cggtaaatcg aagatcgtca aatgcccttt gattgctgtt gctaaaatat
    20521 cgatccgttt atccacacct tttgccccaa caccttgtgc accataaatc tcgccagtct
    20581 ctggatggaa gatcaacttc agtaaaatat ctgttgcttc tggataatag cttgcatgat
    20641 cttttcctga tacatgaatg acttcaaaag acagttcgtt ttctttggct gttctttcac
    20701 ttaaaccggt ggatgcggca gtcattccaa acgttcgtat aattgctgtt ccaataccgc
    20761 cttgatttct tcgagaaatt ccggaaatcg tgtccgcaac ttgtctacct tgacgattgg
    20821 ctggtgaagc aagagaaata agtgcatctt ttccagtgat ttgctgtttt acaacgatag
    20881 catcccccac cgcataaata tcaggaatat tcgtttgata acgttcgtct actaggatac
    20941 ccccgcgtat gcctaattcg attccagcgg tcactgctag ctggttatca ggacgaacac
    21001 caatggataa aataactaaa tcagcaggga tggtacgccc atcatccagt cgcaatcgtt
    21061 tcccgtggtc ttcaaatcca gcgacagcat gagaagtaat cacttctact ccttgatgag
    21121 acaattcttt ttctataaaa gctgccattt cttcatccag tgttggaaga atatgtggtg
    21181 cttgttcgat caccatgact tctaaacctc ttctttttag gttttctgcc atttccaatc
    21241 cgatgaatcc tgcaccaacg atcacggcac gctttggctg tttttctaat gcatgcatca
    21301 ctttatcgat atctggtaca tttctaatag aaaaaacatt gtcggcttct gttatccctg
    21361 gaatcgatgg aacaactggt tttgcaccag gagataaaac caatgcatcg tactgttcaa
    21421 tgtgtttttg accctcatgt acgacttcta ccgttttgtt ttcgggaaag atggctgtca
    21481 cttcatgatt tggacgcaca tctaaacaaa aacgagcgga taatgattct ggcgtttgga
    21541 caagaagatt ttcccgttca ctgatttctc ccgaaagata ataaggcaag ccgcaatttg
    21601 caaaagaaac atacggtcct ttttcaaaaa caacgatttc tgcatcttcc attaatcggc
    21661 gaagccgtgt agcagcagac attccacctg ctacacctcc gacaataacg attttcatct
    21721 tatttccctc ctactgttcg atcaaaccat tgattcatgc caccacgtac attcactaca
    21781 tcataaccag atttctttaa ttctttagct gcttgtttac tcctcatccc tgattgacaa
    21841 atcacataga cggtcttttc tttgtctcca tcatatctgt ttatactttg taatggaaca
    21901 tttttagctc cttttatatg cccgccccgg tattctgcag gtgttctaac atcaagtaac
    21961 gctggatttt ccaaaagctt ttcctttaat tctgggactg ttatcgattt tattgattga
    22021 aatagcaaat tcattttctg cacctcattt acatttttat aaatacctca tggggtatat
    22081 taaacaggag ctttattaat atgtcaacca gaatgactct tcattttttt cactactttt
    22141 tatatttgca taaaataccg ataaggggta ttatctaatt ataaaaatag aggagaatgt
    22201 gttatggaga ttgataataa aaatatcctg aaccgtttga aacgtacaga gggacaaatt
    22261 cgtggtatcc aaaaaatgat cgaagacgaa aaagaatgta tggatgtcat cactcaatta
    22321 agcgctgttc gttctagtat tgatcgcgta atgggcatga tcgttgcaga taatctgaaa
    22381 aattgctttg agaaacctga tagtaatcca gaagaacaag cggctaaact agaacaagcg
    22441 atcaagatga ttattaaaaa gtaaaggaat gatttcaatg aaagtcgttt tccatattga
    22501 tgaattagaa aaatggtcag aaacagggaa aaacgtaaaa aacctgataa aagcttctcc
    22561 agaaacgacg attgtcgtat ctgtaaatgg catcgcgatt accggttatc tagactctgc
    22621 caatgcagaa ttcttagatt tgcaaggagt cgtttttcac gcttgcgcca atgctatgcg
    22681 tgctaatcat atttcagaaa gttctttacc tgaacaagta atcgttgtac cagcaggtgt
    22741 ccttgattta gttgaactgc aatcccaagg ttatgcttat atcaagcctt agtcagtaga
    22801 ggttgcgaca gaagtgatca gcttcaagaa atttcggctt atttttcgaa gaagctactt
    22861 ctggaaaacc gtgaatcaaa ggttgtgaaa acgctgtcct gcatcaagta ataagaacag
    22921 ccaaacaaaa atagcttctc atatttttcg tttggttggg cttattaccg cagatgcaag
    22981 cttttgaaca ccgtttaagg aagaggttgt gaaatcagcg gtttgaccta agtattagag
    23041 aaaaaaacgg gaaaatagct cctcatattt tgccgttttt tgagctaata ccgaaggtca
    23101 gctgattgaa caccgtttaa ggaagagatt gtgaaaaagt cgcttagttt cgagtattaa
    23161 gaaaaatttt gtgaaaatgg atttctacat ttttgcataa atttggctta ataccgagaa
    23221 actggctttt gaataccgtt taaggaagag gttgtaaaat cagcggtttg acctgagtat
    23281 tagagaaaca aacggaaaaa tagctgctca tattttgccg ttttttgagc taataccgaa
    23341 ggtcagctga ttgaacaccg tttaccaaga ggttgtgagc ctaccattta actccgaatc
    23401 acaagaaata atttcaaaaa caacttctcc tgtttttggt tcggttggct tattatcgag
    23461 aagtagctac tgggagtcta tttatcaaaa aaaggaatgc gacataggtc acaccccttt
    23521 tttattttct ttgaaacatc tttcggtact cgacagcata caagacaaaa gattgtaaaa
    23581 gaccgtaagt gatgacggca aaaatgactc tcacccacag cgttggtaat tctgggaaaa
    23641 gcagcaaaag gatcatgcca acatagatga cagcagtcgc tactttgcca taccacttcg
    23701 ctccacttaa tttgatattt ttagatagcc cgattaatcc agctacaaac ataaagcctt
    23761 cttttatcaa gaatagacca aacaacagcc aaagccacgg tcgataccat gccatagaca
    23821 aaatcagaaa aagttgtgtc aatttatctg caaacggatc aagcaccttc cctaagtctg
    23881 ttacctgatt atatctccga gcaatgaccc cgtctagata atcagacaaa cctgagacaa
    23941 caatgaccgc tcctgctata taaaatgact gtctcagcac aaaataaaga taaatcggca
    24001 gcaaaaaaat acggaatatc gataaaagat tcggaatcgt ttgtatttct ttacgccagt
    24061 ctgtgcttaa tttcatttta tcgccctacc tcctcagcac tcttttcttt ttattttagc
    24121 ttacacgggg aataaaacca atgatttcac tgtttttatt tttctttaca gacactgata
    24181 ttatgagcta atttgagata atcacttgct gttttcatcg tttactcttc tttcttcctg
    24241 tcgtttgcgc atttctttta ctagtacttt atgagctttt tttgatcctc gctctaggtc
    24301 acgctgccgt ttacgtgttt tataatcgat aaacagactg tctaaatcgt aacctgtcgg
    24361 gtacagatct tctgctgaac gcaccagacg aacacgctga cgaggcacgt ggattatttt
    24421 tttgtctaaa tatacatcaa tcgtatcttc acctatatct tcataaacta acgcttcttt
    24481 ttggtattca ttgacaaata tccgatcacc ttttgcaaag agctgacgtt cttttttatt
    24541 ctccttccta tttgtaactg ctgtcttagg aagaaattct tttttctgtg tccgataggc
    24601 tttttccgcc aagtaacgct ctgcttgatg gatcaatttc atcgacatat ccattttatg
    24661 agcaatccac aaagcttggc tttctcctgt ttcttccaac ccgcaactga tatttaggac
    24721 gaagtgcttc ccgatcgaaa gccatagcag ccggcacaaa atcttcatga tctccggcga
    24781 atttcttgat ttctccataa tgggtcgtgg caatgatcaa tgccccttgc tcatacatag
    24841 attccatgat tgcgatcgct agtgcagccc cttcatttgg ctctgttcca ctgccaatct
    24901 catctaaaag taccaacgta ttccttttta tctttttcaa aatagcagcg atgttttgca
    24961 tatgaccaga aaatgtactc aacgcatttt ctaaattttg gtggtcaccg acatcgacga
    25021 aaatctcatc aaagacagca agctctgtcc cttcttttgc cggaacttgc ataccgaaca
    25081 tcgccatcaa agtcaataaa ccgactgtct tcaacacgac tgttttgcct ccggcatttg
    25141 ctccggtgat aatcaagcct cgatagtctt taccgatttc aacatccaat ggtaccgcat
    25201 gctcaggtaa aaatggatgt ctgccttgct tgataatgat atgttccgat ttattgattc
    25261 taggtgtgct tccattgatt tctcggctaa attttgcccg agcaaagatg atatctaaca
    25321 ccgtgatcgt ttcgatcaat aaatcgatca cttcttcgtt ttctgcgata gcaccagtca
    25381 atgccgccag gacttgatat tcttcactga tttcttcagc ttttaacaac tgatagtgtt
    25441 cattcaattt agaaatcaca gttggttcaa taaaaaccgt cgttctttta tttgattcat
    25501 cgataatcgt tccatctatt ttatttttat agctggcttt gataggaatc gtatagtatt
    25561 cgcctttttg aacaatcatt gcttcttgga tcatttcttt gtttttcgga tgacgcaaga
    25621 atttcagtaa ttttgactgg atttcttttt cgattagttg taattgtttg cgtactttac
    25681 gcaaattacg tgacgcgtcg tcactgactt tattattttt tattttctgg tcaatcaact
    25741 cttctacatt tatcaaatca ggaagatgtt tgctataagc aaacaacaag ggtgtctggt
    25801 attgattctt atcaaaaaac tttgtgatca tccgactgct tctaagaaaa tccgcatatt
    25861 cgatcaaatc ggttggctgc agaatcaacc cttttttgac ttgttctgtc agtgcatcga
    25921 tcctcggcaa ccccataaat ggaacatgct gactgctttc taagatcaag cgtgcttctc
    25981 ttgtttcttc ttgacgatcc aagacagttt gtaggtttgt cgagaccgtc atatcgctga
    26041 ttcgtttttt gctatagttt ccaattgctc ttgcttggat ttcctttttg atccgatcaa
    26101 attgtaattg actaatcatt tcgttgtcca tattctctcc ttttcttgaa gagatacctg
    26161 tcccaaaata aataagggcc tagagataaa taaaaggata gaacacagct taaaaaagtc
    26221 atgtcctatc cttattagaa tacaaagata ttgttttcct gacaggtacc gtttcataag
    26281 cacaccaaat aaactttgtt tattggctat tcgacttact gattagatac cagctacact
    26341 cacaaaacat accccattct tctaggtctt atttattttc cacttaacta tagcaagaca
    26401 cattttggtt gtcaaacttt agtatgtttt atagatttac accattaatg aatagacacc
    26461 taccgcgata attgctccga tcattggacc tgcgattggt acccatgaat atgcccaatc
    26521 tgaagttcct ttattagcta ttggcaatac cgcatgtgct aatcgaggac caaagtcccg
    26581 agcagggttg attgcatatc ctgttggccc tcctaatgaa agaccgatgg ctaagatcaa
    26641 tccacctaca gcgaatacgt tcgttccagg tgcaaattcg ttttgtccaa aagctagcaa
    26701 cccaagaact aatacaaatg taccaattaa ttctgtgatc acatttgctg gataattccg
    26761 tatagcagga cctgttgcaa acgttcctaa aatcgcgctt tcatctttcg ttgcattcca
    26821 atgtggcaaa taagaaagcc aaacaagaat ggcccctgca aaagctccta aaacttgcgc
    26881 aatgatgaaa ggaacgacaa gattccaact aattgcccct gtcatcgcca tagctagaga
    26941 gactgcagga ttcaaatgag ctgggcccat gaatccagaa atatagacag ccattgttac
    27001 agcgagtccc caaccaaatg cgatgacaat ccagccagaa gcctgtgctt tacttttgtt
    27061 taaattcaca gccgcacaca caccatctcc aagcaaaact aggatcagtg tcccaataaa
    27121 ctcccccatg atttgagtca tatcactgtt catttatttc tcccccttta gttcccgcag
    27181 atctgattct gcaatcaatg cttctaaacg tttagtctgt tgcgccttct cctcttctgt
    27241 ccattcaaaa tatccagcaa ttgcatcgat cacaggttgc ttgatctcat caagctgatc
    27301 tcgttcaaac aacagatgat ttgtacgacg aatgagataa tcgcctgggg ccaaaaccat
    27361 ttcttcttct aaaccataac gtaaccgagc tgactcagcg agactcaaac caggataagg
    27421 tgccatttct tttgctaatt caaagatacg tcgggcatta gtcccgtaaa aatcagcgat
    27481 ataagtagca tcttcttcct ctaaaccggc tgctactcct tcttttgcta attctgtcac
    27541 tgtttcttct aatttagttg gatcgaagtt tccacctgaa atctgatatt ttttagagtc
    27601 gatttctttc gtctctattc cgtattcttc ttttaataat tggcgtatca atcgcaaagc
    27661 tccttctgcc atcttacggt aatcggtgat tttcccacca gataaagtca gcaaaccatc
    27721 tggttctctt tctaaagaac tacctctgga aatcgtagaa ggtgcttttt catcacggct
    27781 gttttccaaa tggttcaaca cgtcttctac ttcagcacga gaaactttgt tttccttata
    27841 ttcacttact gtatcgacaa ctttattgaa gctcttgtct gaaatcgatc cattatctcc
    27901 accattgtaa tcagaaccag aattaccaat caacagtgga cgaagtcctg cccagcttgc
    27961 ttcgatatca ttcaatgtga tatttgcttc tggatagcga tgattgatca catccaatag
    28021 ataatctaca tcttcttgtg tgactttcgg atccgtaaaa tctccttggt aatccgtatc
    28081 tgtcgtacca aagtaagtct tgttttctct tggaatagca aaaaccatcc gcccatcttg
    28141 ctttcctgta tcgaagtatg tgggttgcgg tacaggcagt tttttcgcat ctacaactaa
    28201 atggatccct ttagttggac gcattttagg agagactgga cgcgtaaaat tcaagtttcg
    28261 tactttatct acccaaggac cactcgtatt gatcactaat tttgctttga tttcgatcac
    28321 ttcatctgtc agcaggtcac gagctttgac gccaacgatt tgatcgccat catataaaaa
    28381 gccaaccgct ttcattttac ttactagata agccccatct tcggcagcct ttttgatatt
    28441 atcaatcact aaacgggcat cattgttgcg gaaatctaga tagacacccg cgccttttag
    28501 cccttctttt ttcaaaaacg gttctctttc caatacttct tctggtgtca gcgtataatt
    28561 ctcatatttg gttcctgtca catttgccag tttgtcataa aggtccattg ctactttgac
    28621 cgagaacata ttgaaggttg ttgctccttc gccctcatag attggcaaaa gcattggatc
    28681 tggttttggg atgtgtgggg caatactttg tacgactgca cgttcaccaa ctgtgtcagc
    28741 tactacttcc acatcaaatg tcttcagata acgaataccg ccatgaacaa gtttggtcga
    28801 gcgagaggaa gtcccttctg caaaatcctg catttcgatc aatcctgttt tgattcctga
    28861 tgctgctgac tggactgcca caccggcacc agtgattccg ccaccgatga tcaacagatc
    28921 caattcttct tgctgcattt tttgaatgct atcttgtctt gtcttgtttg aaaacatatg
    28981 gatttcctcc ttattcaaat gcttgggtag cttttactgc ttttttccaa ccgttgtaaa
    29041 gtttttctcg gcgctcttcc tccatttgaa cttcaaatag cttgccagct tcataaaatt
    29101 cgcggatctc gtctgtatct ttccaataac cgacagctaa tcctgctaaa aatgctgccc
    29161 ctagcgccgt tgtttccaag ttttctgctc gttggatcgg tacattcaag atatctgttt
    29221 ggaattgcat cagatattca ttgtttgctg caccgccatc tactttcaat acaggaatct
    29281 tgattccagt gtcatcttgc atcgtatcga tgatatctct cacttgataa gcaatcgact
    29341 gtaaagtagc tttaatgata tcttcttttg tcgtaccacg tgtcaagcca aacatagccc
    29401 ctcgtgctgc ctgatcccaa taaggagcac ctagcccaac gaatgcagga acaacataaa
    29461 cctcatcttc gcttgtagag cgtttcgctg cgtcttctga atcgctagct ttttgcaaca
    29521 tttgaagtcc atctctcagc cattgaatcg aagaaccagc cacaaaaata cttccttcta
    29581 aagcatagta aacctttcca tttatgccgt aaccaattgt cgtcaataaa ttatttttgg
    29641 ataattgcgg tttttcaccg gtattcatga caataaatga acctgtacca taagtattct
    29701 tgaccatgcc tggttcgaat gccatttgtc cgaataatgc ggcttgctgg tctcctgcca
    29761 tccctgaaat tggtgcttcg cttccataaa aatgataacc ttgcgtataa ccataaactt
    29821 ctgagttaga agtgactttt ggtaacatca ctcgaggaat atttagtaaa tctaaaatct
    29881 cctgatccca atctagctga tggatattga acaacatcgt ccgactggca ttcgagtaat
    29941 cagtaacatg tgcttgtcca tcggtcagtt tccatactaa ccaagaatcg atcgttccaa
    30001 acatcaattc gcctttttca gctcgttctt gcgcgccttc tacatgatca agaatccagc
    30061 gtatcttcgt tgctgagaaa taggcatcga tcactaaccc tgttttttcg tggaaaaatt
    30121 cttgatagcc ttcatttttt aagttatctg caattccagc tgactgtctg gattgccaaa
    30181 caactgcatg atagattgga cgtcctgtat ctttttccca tattactgtt gtttcacgct
    30241 ggttcgttat gccaatccca gcaatctgat ttggtttgat ccctgattcg atgaacgctc
    30301 cggcaataac tgactgtaca gaattccaga tttcatttgc atcatgttcc acccaacttt
    30361 cttgcgggaa atattgcgtg aactcttttt gagaactgcc gatgtgtctt cctttcttgt
    30421 caaagataat tgctcgtgag ctagttgtac cttggtcaat cgccatgata tacgttgatt
    30481 ctgtcattgt gattccctcc attatatgca aacgctttct ttaactactc ttatcatact
    30541 actgttttaa aaaaagaaac tgtcaaaatt ttattctttt tttctttttt tgacagtttt
    30601 ttattatgaa gctatttttt aatcaattct tttctttttt cagcgtctct attcttcttt
    30661 tcaatcgaat aatgtcgtaa ggtgacgcaa attctgacag ataatattca cactgttttt
    30721 tgtttctctg gcaaaatgtg atcaccaaat catattcttt accttcttca taacttccaa
    30781 tctcaatacc gccgattccc ctcagttctc tgatcagata ttgttgaaaa gcaatacgat
    30841 aaatcggcag actttctaaa tcgattccaa tcactactgg ttttgcaata taaaaatcga
    30901 tcatcaaaag aacattggca tactggatca tcaggtaact aagctcacta tcttcagctc
    30961 ttttcctcgg caattgctgc accgctgttt cctgtaattt cgccagtaac tgtgttaagg
    31021 aatgcccgag cattttctgc tgctgttcca aaagccgatc acgctcatag acttcgatct
    31081 ttccaacaaa gaaaaaccat tcattgttga tctgggaaat ttggtagcct actgcctttt
    31141 cttctttgat tttcaaacga cgaggacgat aatgccataa catcgtttcc cgaatgtaag
    31201 tatctaaaac agcggttggt aactttttgc tccgtgtcaa atcatactga taaaatattt
    31261 cttcgtctac tatcgaaaaa gaaacaaaaa agctgtaaaa catcattgtt tcgtatacat
    31321 tcagttctgc tgctgttcga ctaagatgca tcacgatgat tggatcgatt gcttgataaa
    31381 gccggtccga ctgatagagt aatttctttt ctttcaaagt tgcgtacgta cttttttcat
    31441 tcaatagtct ttttttcgta atcccgagcc agcaagcaat ctgtgcttct gctgatgccg
    31501 taaacgttgt ttctaataca cggtccaatc ctagaatgaa cggtcttttt tctggtgtat
    31561 tttgcaaaaa aagcggtcgc tgatcttccg ggatatattg gaatagttca taataaaaat
    31621 atcgtatctg tagctcttct ccgtataatt gaccgttttt gatttgtaat ccaaattcct
    31681 ctaagagctg gttcaattct ttgatctttc gaaaaacaga agactcactg atattaaacg
    31741 ctgtcgctaa tcgaacgatg gatacttgct gatgttctaa taaatagacg agcatcttaa
    31801 acttcaagct ttcctgcaat aaaaacaata gaattttttc caaacttaca tcttcgtcca
    31861 acacaagcag cgcctgatac tcatttcgct gtacttctac ttttttttcc atcattcggc
    31921 caatcaattc aatatcttcc aaatattgat caaacgaggc ttttgacagt tggacatgct
    31981 ctctcaaacc attgatagag gctgttcccc cttgcagaac aaggtttcgc aaaatcagga
    32041 tctgtaactg ctcttttctt tctaataatg cttcaatctt cataggcagt attcgccacc
    32101 tttgaccaca ccatatctgg cacacggtat tctggctgga aaaatagctc atgttcttct
    32161 aactgttcaa acgtccatcc ttcttctact gcaattgtca gttggtcaag aaggtggaac
    32221 atctcttttt tgctttggag ctgtgctcca agaatctttt tttcagaatc aaatagaaat
    32281 ttgagtttaa ctttgctttg atttattgct gtaagcttgt agtctacgta tcctgtttca
    32341 attttttggg gataaaaaat gctctcttct tctgtcaaac ctacgcttcc taagaaatag
    32401 ccaaaccact gattgccgac tggacgatag gttccctgat cctttgtctg tgagccacta
    32461 attgtcttgc tgacatttcg tgctgtacgg acagcgttat ttactagaga aacatacatt
    32521 ttttcatttg ttggacgaaa agttacttgg atcgcatcgc caatcgcata aacgtctttt
    32581 tgagacgttt gcaagtagtc atctacccag atcgttccat catcattcaa tgttaattgt
    32641 tcttcccaca tctgattgtc gggacgagaa tgattagcca gcaaaacaag atcatagttc
    32701 tcctttcttt tttccgtctc tacaatagcg gtttcatttt cggtcaaaga cacaacttgt
    32761 tcttctaaaa aaagtgaaag cccttgattt ttcatttcct ttactaacgg ttctgtcatt
    32821 tcaggatcta gataacgaaa aagaattgac tttcggcttt cgtaaagatg gatttgtttt
    32881 tttcttgatg ccaacccttc agctatttcg attcctgctt gtcctgcacc aataattgca
    32941 attttctttg cattcatgat cttttgctga agagccgtta cgttttgtat ctttttaact
    33001 gggtgaataa gcgttgattc tgcagaaata tttcgaaact tttgatttga accagtcgct
    33061 agaatcaaac gatcaaaatc caattttttt cctcctgcta aaacgacttt tttttctttc
    33121 aatcctatta ctgtcttttc tgtgtaaaca tggattcgat agcggtcagt cagttcttct
    33181 ttcgtgatcc aagaaagttg tttttcactg gtaacctttc cttttaatat caaattgaac
    33241 ccgctgggaa tccatcctaa cttcgattgc ttttcaatca aatatatttc acttgttggc
    33301 atcaattggc gaagataaat agccgcatga atccctgcaa acgacccgcc gacaattaca
    33361 atgttcatct gcattcctct ttcttataag gtgttctact gctaacttct caatcagaaa
    33421 acacagtatt cataactcgc tttcaacctt atgatacata gcgatgcagc agaatcaacc
    33481 ctctccatca taccgtgttc taggtctgct tctcaataat gtactagtgg acctaattga
    33541 agagtatcat ttctgaagag atgtttcaaa aaatgtactc ttataaaaga aaaaaactcg
    33601 ccttttacgt acctctgtcc aaataggaaa cagaaaagaa cgaaaagacg agttttagcg
    33661 tgtgtaatgt aatagttaga atagacacat catttatttt taattcttta atgcttcaac
    33721 aactgcttcg cctaattgat gagcagattg aggattttgc cctgtaagaa gccgcccatc
    33781 aatttgaacc acttctgaaa aagctgctcc agactgatag tttgctcctt tttctttcaa
    33841 agcatcttct gctaagaatg gtacttcatt cgttgtacca tttgcttcct cttcttcatt
    33901 actgaaccca gtcactgtac gtccagctaa aatagatttt ccagactcat ctgtaactgg
    33961 caacaatcct acaacaccgt gacaaacagc tgagaccagc ccttcatttc gatagatttg
    34021 tgaagcgact ttcccaagat ctattgcttc tggaaaatcc cacattgttc cgtggccacc
    34081 agcataataa accgctgcat aatcatttgc atccacatca tcaggacgta aggtatttcc
    34141 caaggcccga tttctgaaat cttcatcttc ataataggcc caatcgattt catccatatt
    34201 caaaacactt tgcggttcta aaggtacata tccgccttta ggactgacaa aatctgcatc
    34261 gatcccattt tttactaata catcataaaa atgagtcagt tcacttaacc acaaacctgt
    34321 aggacgctcg atcttttcgt acttttctac atttgttaat acgatcaata ctttcatttt
    34381 cccatcctct ttctataaaa attgcgttat tgataacgtg ctttcaagaa attatataat
    34441 gggcttggca aataaagctc tttatatgct tcttcaaaag gtttcgttac aaattcatcc
    34501 tcacggtttt ccaataattt ctctgcccag tcaggatcga cgatcatttg ctgtccaatt
    34561 gctgcaagtt ctgcatattt caatacctgc tctacatctt cgcgattgcg gatcccaccg
    34621 acaccaacta aagggatctt tccttgaatc gtgttatgga ccacttcaag aatcgattta
    34681 tcgttgtatt tatcagaacg agctattcga tcatacatat ttaatgaaac atgcagataa
    34741 tctagttttg actcacgtaa tcgttcaaga agccagatcg tgtcttcaaa acggatacct
    34801 ggtgtttcat attcttctgg tgagaaacga taccctacga tgaatggttt tttagcatat
    34861 tgttcaaccg ctgagaaaac cgttgttaat aattcttcaa taaaacgata acgattttcc
    34921 aagctaccgc cccattgatc ctctcgtcta ttcgaatgcg gagagaaaaa ttgttggatc
    34981 aaatacgtat ttgctccatg caactcgatt ccatcaaatc ctgattcaat cgctctacgt
    35041 gtggctttgc caaatgcttg gattgtttca acgatttctt cttcactcat tgctcgtggt
    35101 gtttccgcat caggacgttt tgctgcaact gcacttgcag atacaggctg ttctccagat
    35161 aaagttgctc gacttgtcat tcgtccaccg tggaaaatct gaataatcgc ttttgcgcct
    35221 tctccttgga ttgctttcgc tagctcactt aatcgaggaa tcatttcatc atgagcgatg
    35281 ctgagttcac ctggccagcc ttttccgata tcttggacat ttgcagcacc agtaataaca
    35341 gcaccaagac ctttagaacg ttgtttataa taagtgatct catcttcggt gatcactcca
    35401 ttgaaaaagc tgagttgtgt cgtcataggc gacagcatca atcggttctt caattgaact
    35461 cctgaaggta aagtaattga ttgatcaaat tgtgacattg tcattcctcc cataattcgc
    35521 tctgattaac ctatcgagaa tcatctatca ttaaaccact cgattagttg tacatgcaac
    35581 taatttgctt aaaaaatttt attttttttg taacttatct gttgcatcat tcattgtctg
    35641 ggctaattct ttacttgctt catttcccaa aactgctttg tatgactgat gaagttcttc
    35701 ccagctacca tcgacttcct tagcaaattg gcgtccttgt tctgtaagat aaactaatga
    35761 caagcgacct tcgctaactg ttttgacgta tcctttatat tctaattttt caacaaaacg
    35821 agcaatcgtt gatggggcgg aatagagcat ttgtgcaata tctttttgtg gtattccatc
    35881 tttttctttt aatatcaata aaataaaacc ataagttggt gccagacctg tccttctaaa
    35941 cgcttcatca gctaatttat tcaacatgcg atctagcttt ttcacgctaa agaaaagaca
    36001 ttcatctaac gctttttcaa ttggcttttc catttcatcc cctccattgg cgtacatatc
    36061 atagtatgat ttttattttt tgtcaaaaaa cacggaataa tgtccgcctc ctctgtctag
    36121 atttgaaaaa aagaagtagc catcttcaaa gaatttcggc ttgttttcga caaaactcct
    36181 tctggaacac cgtgaatcag aggttgtgaa aaagtcgttt agttttgagt atgaagaaaa
    36241 attttgtgaa aatggctttc cacatttttg cataaatttg gcttaatacc gaaaaactgg
    36301 cttttgaaca ccgtttatat cagaggttgt gaaaaagccg tcctgcatca agtaataaga
    36361 acagccaaac aaaaatagtt cctcatattt tgtcgttttt tgagctaatg ccgatggtca
    36421 gctgattgta caccgtttat tgagaggtcg tgagcctgcc gttgaactcc gagtcacaag
    36481 gagcaatttc aaaaaacagc ttctcatgtt ttatgactat ggtgacttgt gatcgaggag
    36541 ttggcaggag aatactgttt attcgtaaag aaggggctgt gacaagactt ttgtcacagt
    36601 cccttatttc cttttattat aggatttcgc catttgattg gatgacttgt ctgtaccaat
    36661 caaatgattt tttctttaac cgtttctttg tgccattccc ttcgtcatct tgatcaacat
    36721 agatatatcc ataacgtttt gacatttgtg aagtagaagc gctgacgata tcaatgcagc
    36781 cccacgacgt ataaccaaat aaatctactc catctttgat agcttccttc atctgctcaa
    36841 tatgctgctt caaatagtca atgcggtaat cgtcttgaac ggttccatct tcttctactt
    36901 tgtctatagc tccgagtccg ttttcagcta tgaacagtgg tttttgatac ctatcatata
    36961 attgattcaa agcaattctc aaacccttag gatcaatctg ccagccccat tcacttgatg
    37021 gaagatacgg atttttaatt cctccagtta ttaagtttcc ttggacccct tcccaatttt
    37081 ctggatgagc actgactgca ctagacatgt agtagctaaa agaaacaaaa tcaacagggt
    37141 aggctgctaa gatttcttca tcacctactt ctatttttaa gttgacacct ttttcttcaa
    37201 acattcgttg cgtatacgct ggataatatc ctcttgcttg aacatcacta aagaacaaag
    37261 aagttctttg ctgctcaatt gttttttgaa tatcatccgg atcacaagaa tacggaacaa
    37321 gaagctgata gctgatcata caaccgactt gtgaaccttt gataatttca tgacaagctt
    37381 ttgtagctaa agcacttgct ataaactgat ggtgtgcacc ttggtatttc gcttgttcca
    37441 aatggggatg gttttcttca attactccta cgctaacaaa agggtgatgt tttacacagt
    37501 tgatctcatt gaaagtcatc caatatttta ctttattttt atagcgtttg aatactgttt
    37561 cagcgaaacg aacgtaaaac tgaataagct ttcgattagg ccatccacca tattctgtaa
    37621 ccaaatataa cggcatttca taatgggaaa gagaaacgac tggctcaatc ccatatttta
    37681 gacattcgtc aaacacttga tcataaaaaa ataatcccgc ttcattgggt gtttcttcat
    37741 caccatgagg aaaaattctt gtccatgcga tagacatccg atagcattta aaacccattt
    37801 cagcaaataa acggatgtct tctttaaagt gatgatagaa atcatttccg tgtcttttag
    37861 gataataata cttgctgttg ggatcctgcg ccgcttttac ttgctgatgc gtcatcatat
    37921 gccattgatc tgtccatttt tctttaggta aatcgctgtc gaatgtatac acatcagaca
    37981 cagataatcc tttttcatct acatcccatg caccttcaca ttgattggcc gctgtcgctc
    38041 cgccccataa aaatccttct ggaaattttt tcatatcgat ccccctaaac ttgctagttt
    38101 tcttgtttga ttatttttct tatgactagt aatgatattt ttgaatctac tactatttgt
    38161 aacaataacc attgtggtcg gatcatatcc ctctttgatt aattcttgca aatctacttc
    38221 catcaataaa tcgcctttac gtactttttt tccatcagat actgcacttt gaaagtattt
    38281 gccatttaaa ttcactgtat caataccgat atggatcagt atctctgctc cttcttcact
    38341 gactacccca attgcatgtt tcgttggaaa aactgttgaa acttccccgt ttacagaact
    38401 gtagattttt ccgtccgttg gaatgatcgc aaacccttct cctaaagatc cactagcaaa
    38461 tgttggatcg tttacttcag ataaatcaat tatttcacct gctgcaggag tacttaacac
    38521 tatatccttt gaggattctt cttcgatttc atccttgtag ccgaaaagaa aagttagcat
    38581 acaggaactg atcatcgcta agaaataact agcaactagc caaacaaatc cttcaccaat
    38641 ataaatcggg aaagacaaaa tacttcttgt agcaactgcc aaagcaccgg ctcctgcggc
    38701 accagctact ccaccaaata tagctccaca tatacaagca atgtaaaatg gtcgtttata
    38761 tttaagagtc acaccataaa cagcaggttc agtgattcca aataatgctg tgataaatgc
    38821 agataaagat aaacttttta ctttcttatt tttcgttttc aaaaataccc caagtactgc
    38881 tcctgcttgc gcagctacgg cagtcataca tgctggacct agcgtatcac gaccgtagaa
    38941 agaaagattg ttggtcatta ctggtacaag tccccaatgt aacccaaaaa tcactaacac
    39001 ttgccaaaat ccgccgataa cagcaccaga taaaacaggg ctaaagttat aaacagctaa
    39061 atatccagct gcaatcagct gacttgcata atctgcaatt ggacctacaa ctaataaaat
    39121 aattgggacc atcacgatga tacaaatgaa tggtgtcaat aaatttcgaa tactttcgtg
    39181 caagcttttg cgaatcttag gctcaatata agaaagaacc catacagcta aaattgctgg
    39241 aataaccgta cttgtatacc ttgctagaat cacgggcatc cctagaaatc gcaaggttat
    39301 gctatcacta tatgcactga caatcgtagg ataaacgaga gctgctgcaa taaccatagc
    39361 gatgaaacga tctgtattga acttttttgc agcagtatat gccaaaaata taggcaagaa
    39421 atagaaaaat gcatcagctg ctgcattcaa aatgatataa gttcctgatt cagtagttaa
    39481 taaattcaaa ctcgtacata aggcgagtac tcctttgatc aaaccacttc ctgccaagac
    39541 gcctaaagtc ggggcaaaaa cagcggcgat catgtctata aaagagttta aaatgccttt
    39601 tttctcagtt cctctctttc cactagatgc atcatcttct agtttcattt gtttggataa
    39661 tacgtcatac acttcattta ctgcatttcc tactacgact tgaaacattc ctccagcttc
    39721 gactacagtg atcacacctt ctagttgctc taccttctcc ttatctgcct tcttattatc
    39781 ttttagggta aagcgtaacc ttgttgcaca atggtaaaca ttgctgacat tttcttcgcc
    39841 tccgacaaat cggacaatat ctttcgctaa ttgtgaatag tccattttta cttccccctt
    39901 gttaattaat gccgtagcaa tccttcttac aagataagta tagttcttag aaaacggttt
    39961 ctccattcaa ttagtacccc ttctaatagc gaacgaaact gtacaatagt gtgtatggtt
    40021 tacttagccg agcaaatcac tcactgcttg aaagaaaaca agtatatcgt ttctcttata
    40081 gtcaaaggag acaaaaaaaa cacgcttgat cttcctttgc tgatgtataa gtggtatctc
    40141 aagctgatac tctgtgattt ttgtttctac ttggcttttg ataagacagc acaacaaagt
    40201 ctcggcattt aacggaagca taactaggtc agaactagga gagattagtt gctgaatgga
    40261 atctatcttt ttatggattt ttatttgatt ttcttctttg tagtaatgat caagcagttt
    40321 tgttttcttt ttttcttcaa ctatggtcag cttttcacgc aaaatctgat aaatctccca
    40381 agattcaaac tcatctagaa cactaggtag ataaatcaaa tcatcccact caaaaataag
    40441 ctcttgctca gtagtcaaaa ttgcatcata ttccgttatt ttgtttgatt gatttttgaa
    40501 aagatacact ggttctatcc ggcattcttt gatccacatg ttcatacttt gttgaatctg
    40561 tttttttagt gtattcaaaa tactaattgg ctggttacta acaatcagta tctccccttg
    40621 ttttcgaaaa ggattcaacg cttcggcaag atacaaaaca aaataagcgc tttcagctaa
    40681 attgggacgt aattgaaaat gtattgggaa gaaacgccga attaaataaa tttctaaagg
    40741 ataacgggag atgatctttt gtacaaaatg attcatggta ttatgtccgg catccatcct
    40801 cgttttcatt tgtttgatat gggttagcaa aaattctgtt tcatggaaca ataacgaaac
    40861 aggaagatct aaaaaacgaa taatcgtttg ctcaaatgct cttagattct tctctatttc
    40921 tttgtttttt tcatcattaa tcatcaaata atttaactct aatagtcgat tttcaattgc
    40981 ttgtttttct tgattggaaa aagaataggg tagttctgat gataactttt ttgtgagggt
    41041 ctctaccatc ggaataaata atgtatacct gtttgttttt tctaaaagtt cttctcttcg
    41101 aagaatggtc aaaacgagat accgagaaa
//
LOCUS       DANKKG010000010        38372 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000010 DANKKG010000000
VERSION     DANKKG010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.010"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(<1..1537)
                     /locus_tag="PW004_000456"
     CDS             complement(<1..1537)
                     /locus_tag="PW004_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HDL2725452.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFE"
     gene            complement(1541..1951)
                     /locus_tag="PW004_000457"
     CDS             complement(1541..1951)
                     /locus_tag="PW004_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HDL2725453.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(1948..2871)
                     /locus_tag="PW004_000458"
     CDS             complement(1948..2871)
                     /locus_tag="PW004_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HDL2725454.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(2868..3746)
                     /locus_tag="PW004_000459"
     CDS             complement(2868..3746)
                     /locus_tag="PW004_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HDL2725455.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(3733..5070)
                     /locus_tag="PW004_000460"
     CDS             complement(3733..5070)
                     /locus_tag="PW004_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HDL2725456.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(5073..6176)
                     /locus_tag="PW004_000461"
     CDS             complement(5073..6176)
                     /locus_tag="PW004_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HDL2725457.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(6250..7758)
                     /locus_tag="PW004_000462"
     CDS             complement(6250..7758)
                     /locus_tag="PW004_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL2725458.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(7724..8416)
                     /locus_tag="PW004_000463"
     CDS             complement(7724..8416)
                     /locus_tag="PW004_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HDL2725459.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(8438..9412)
                     /locus_tag="PW004_000464"
     CDS             complement(8438..9412)
                     /locus_tag="PW004_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2725460.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            9712..10584
                     /locus_tag="PW004_000465"
     CDS             9712..10584
                     /locus_tag="PW004_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2725461.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(11042..13240)
                     /locus_tag="PW004_000466"
     CDS             complement(11042..13240)
                     /locus_tag="PW004_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2725462.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            13472..14464
                     /locus_tag="PW004_000467"
     CDS             13472..14464
                     /locus_tag="PW004_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2725463.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            14524..15945
                     /locus_tag="PW004_000468"
     CDS             14524..15945
                     /locus_tag="PW004_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HDL2725464.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            15938..17437
                     /locus_tag="PW004_000469"
     CDS             15938..17437
                     /locus_tag="PW004_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HDL2725465.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            17476..18870
                     /gene="uxaC"
                     /locus_tag="PW004_000470"
     CDS             17476..18870
                     /gene="uxaC"
                     /locus_tag="PW004_000470"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HDL2725466.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            19029..19712
                     /locus_tag="PW004_000471"
     CDS             19029..19712
                     /locus_tag="PW004_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HDL2725467.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            19714..20550
                     /locus_tag="PW004_000472"
     CDS             19714..20550
                     /locus_tag="PW004_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDL2725468.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            20561..22315
                     /locus_tag="PW004_000473"
     CDS             20561..22315
                     /locus_tag="PW004_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2725469.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(22591..22932)
                     /locus_tag="PW004_000474"
     CDS             complement(22591..22932)
                     /locus_tag="PW004_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HDL2725470.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(23000..25171)
                     /locus_tag="PW004_000475"
     CDS             complement(23000..25171)
                     /locus_tag="PW004_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HDL2725471.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            25340..26239
                     /locus_tag="PW004_000476"
     CDS             25340..26239
                     /locus_tag="PW004_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDL2725472.1"
                     /translation="MVQLVCDCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            complement(26310..27167)
                     /locus_tag="PW004_000477"
     CDS             complement(26310..27167)
                     /locus_tag="PW004_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL2725473.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(27152..29854)
                     /locus_tag="PW004_000478"
     CDS             complement(27152..29854)
                     /locus_tag="PW004_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HDL2725474.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(29867..31156)
                     /locus_tag="PW004_000479"
     CDS             complement(29867..31156)
                     /locus_tag="PW004_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HDL2725475.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            31306..32352
                     /locus_tag="PW004_000480"
     CDS             31306..32352
                     /locus_tag="PW004_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2725476.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            32354..34507
                     /locus_tag="PW004_000481"
     CDS             32354..34507
                     /locus_tag="PW004_000481"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HDL2725477.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(34980..36431)
                     /locus_tag="PW004_000482"
     CDS             complement(34980..36431)
                     /locus_tag="PW004_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2725478.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(36428..38152)
                     /locus_tag="PW004_000483"
     CDS             complement(36428..38152)
                     /locus_tag="PW004_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL2725479.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            complement(38145..>38372)
                     /locus_tag="PW004_000484"
     CDS             complement(38145..>38372)
                     /locus_tag="PW004_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HDL2725480.1"
                     /translation="YTISVPNLLKLSFISVFSSFSSFLKIIIGSGIILGVTWQFKGLI
                     LFGVIGLLTVWNGTMTTHWREELDKQLESYE"
BASE COUNT        11178 a         7877 c         6860 g        12457 t
ORIGIN      
        1 actcaaaatc agcataactt cctctttttt tatttagatc atgcactgct atcccgataa
       61 aatttccagt aaaccctcct gagagtatac acatgttttt ttgcggacct actttcttcc
      121 attcttcagt tgatacgtct ttcaaatgaa actgtgctgt ctctttcttt acagtgactt
      181 tcaaaaaagt cttcttttct gtgagtggaa tcgtccaatc ttctatttgt gctactccgt
      241 tttctgattc tatcatacgt aggcattttc ccttgtcctc attccaagta acataacaat
      301 aaatataatt ggattcattt aagtacagta ccagtccagc catctgatta aaattattag
      361 gcatgtattc aaatgctgtc tccgcatcaa aagtaaaatc cgtttgccga atagctaaca
      421 aatgatgttc gaaggtagat tgaagagatt caccggaaat cagacgtaaa aaacctggtc
      481 tacattttgt atcacaccaa tcagcatttg gcaaaatgcg tctggcattc cagttcttct
      541 ttagctctcc ttcaaatgta tccataaact gatgatcttt ttcttgttgt acctcttcat
      601 ttgtctcaat cattatttct tccatcggag ttgtctggct atctttaagt cttaaccaat
      661 cgtcggacgt ccaatacact tcttgaatag cggtttctct tcccaaaata gccgcaccgt
      721 ttaatggtct ggtacaaaga tatgtcatgt accatcttcc tgacggggtt tgtactaagc
      781 tcccatgtcc tgtacattgc aatgtagaag caggtttgtc actagctgtc agcataggaa
      841 aaccaggatc aagctcgtaa ggaccagtaa tattctttgc tcggcaaact gtaactgaat
      901 gtcctcttcc tgtaccgcct tctgccgtca tcaaataata atacccgtta cgaaaataca
      961 aatgtggtgc ctccgttttc gctaattctg tacctgcaaa tattttatag atgggaccat
     1021 caagcttctt cttctctgca tcatattctt gtaatacgat accgctggat ttattcggtg
     1081 tgtccagacg ataatcccat agttcattga caagccattt ttttcccgtt tcaagatcat
     1141 ggaacaaaga aggatcaaag ccgttactat ttaaataaat tggttcggac caaggacctg
     1201 tgatctcaga tgcagtgatc aaataattgt ggacatcttt aaacggacgg cctgcacttt
     1261 tgacatctgt gtaaacaaga taaaacaatc catcagcata actgatttgt ggcgcccata
     1321 tacttccatt tgtaggattt ccttgcaaag atacttgagt ggtcaaaata tccgttgtat
     1381 gtgaccagtt gactaaattt ttcgactcat atactcggat tcctggaagc cattcaaaag
     1441 aggagacaat aatgtaatac cgctctttca ctcttaatat attaggatct ggattgaatc
     1501 ctcttaaaat aggatttttc actttgatag tctgcatgat tcaggctcct tttctcattc
     1561 ttactcgata taaactgaat gccccagctg cgaaataaaa aaatgcaaaa ggaaacgcaa
     1621 taaactgagt gccaatcaac aatatgaatc ctgttataaa atacaaacca gtggcagttg
     1681 ttaagtattt tcctttagca gtcaaaaaca atgctactgc taaaaaagca agtgaaataa
     1741 acaacacaac ggcaaacaga ccagtcatgt cctgtaataa tccaaatgta ttttctaaag
     1801 acaaagttga atgatctaca gcaagcgact gataactttt tttaaactct gacgcagaca
     1861 tattacttaa agtaactgct aacccgccaa agaaaatcaa agatagactg ccaccgacca
     1921 ttcctagcaa tagttcccat tttcttttca tttctgaacc ccctgctttt ctaaatgtat
     1981 ccattcaggt cttttgatcg atgtttcata attgttatta cgttcagaga atttgacagt
     2041 ctgttcattt ttcttgtcat cccaattatc ccaaccagtt gggtgaacat gcccacctat
     2101 cttacacgct tcaaataaga catttgcata cggacgccac ggtcttccta aatagacgtt
     2161 tctagtatca ggttctgcag tcaaggtaca gctttcaaaa cgataccctg ttttttgatt
     2221 tttcggcgta gatgctgctg tgatgtatcc aggattttta ctggctaagc tatggatatg
     2281 acactgatga aaaacggcat ctgccccacc aaatatgaaa tccaccgttc cttcaatcca
     2341 gcaatattca taggtctgac gacaatactc aggattggca gaagccggtg tacaaaaagg
     2401 agtaccatct ttttgcaaat caggcaacgg acccgtgcat aaggtatcct gataaccaga
     2461 tagtcgacag ttttttaaag atacgcgatc tcctgaattg aataaagcaa cagcctgtcc
     2521 taccttttct cctggacctg ctgtattttc gatgtgaaga ttttctaata cgacattcga
     2581 tccttctaag aaaaaagtag cacttcggaa agtccctaac tggctaccgt caagatactc
     2641 ctgttttgca aaacgattac cggagataac tactgttcct gctcccacaa gtttaaaatt
     2701 ggataatctg ctttctatat attcatcata tcttccttct tttactatca agacctttgg
     2761 atggctatcc ggcaggcctg ccaaataatc gattccttct tggatagttt caaaatccgt
     2821 cggggtatct atagcattct ttccaattat cagtttgtct ttcatcctca taaatgctcc
     2881 cttatgaatt gtttccaaat atttctcatt tcttgtgttt ccatatgacc gcctgttaaa
     2941 gaaaaagaag aaaagttttc tggactaccc attgattgat actttttgtc cagccctttt
     3001 ctcagcttca gtacgccttc gtccaagcac atcgtatcgt tttttccaac taagctaaga
     3061 cgagggcgcg gagcgatttt ttcttggatt gctaacgttg tataagctgt caatagattt
     3121 ggcacatagt aataaaaccc atgatgatct aggcggcgat gtagcaaaag tatttcgatg
     3181 tccacctgtg ccgctagatc aatacaaact ttgacacgag aatccaatgc tgtcacccac
     3241 cagcttaata agcctcccat agaaagacca atcgttgcga gccgttgagt atctgtgtct
     3301 tcccttgtct ccaaataatc gatcaagctg atcacatcat aaatacgcat tccccataac
     3361 gtttttcctg ttaataaaaa ttgtttgaac aactcgcttt cagaaatacc tccgcgttct
     3421 tcaaatcccc aagcatcaat cgaccaaaca gcatatccca ttcctgtcag tacttctgca
     3481 aaagatggag aagctaagta aggagcacca tggatcaatt cacttttccc gactgaaaaa
     3541 tctccgccgt gagaatgcag gtaaaggacc ggcggaaacg gtccttcttt ttctggataa
     3601 gcatagaggg ctggtactgt ttccaattca tttaagtaca attgatagta ttcaagcata
     3661 aaaccgtttt gtttttctcg gtataatcgt tttccactag gggtatgtct gctagatgga
     3721 gcacctaaaa aatcattcaa ctgcataagc aaagttttta cgaacaacca gttcatccat
     3781 attttctgat tgatttgatt ggaatttctc aatactgatt tctttagtat tttctaatag
     3841 aaatgctggt ccctcgtgat tttcaatcga catctggtta aaggaaacct tacccgcaaa
     3901 gccaatataa aatccttgtc tgctcatttc ctctattcct gtcatcatag caggaacacc
     3961 gggaactgca tcttgggcca tcgagacatc gatattgtca aatgtgattt cttgtatgaa
     4021 ttgttccgct aatccataaa taaaacctgc agaagcatgg acattccttg ccgtgatatt
     4081 cgaaaaatgg atccttctga atgctggtgt ccgttcatct atcggataag cctttttttc
     4141 ccatacatac ggttctttcc ctcgcggacc acagaaataa taaagattca gtataaatgg
     4201 acacatcaca ttatccatga ctatattgga tactcgaata tcttcaacga tcccgcctcg
     4261 acctcgtcta gatttcaatc gtattcctcg atcagtttct tggaagatac aatttgaaat
     4321 ggtaatatta cgaatactac cactcatttc acttcctaat acgacaccgc catgtccatg
     4381 gaccatcgtg caatttgtga tcgtgatatt ttcgcaagcg atcctttcgt acgtatcttc
     4441 agtccctgcc ttgattgcaa tacagtcatc tcccacgtca atatggcaat tactaatacg
     4501 cacatttttg catgattctg gatcaattcc gtctgtattt ggtgaatccg ctgggttcaa
     4561 gatcgtcaag ttatcaaacg ttgcattact gcaaagaatc gggttgatcg tccaacttgg
     4621 tgactggatc aatttaatat ctttcacagt aattcgatga cagttatgga aactcatcaa
     4681 tttaggacga ggataagcga gattatctgg ttcatttcta aaagtatgcc accatttttt
     4741 tccatttcca tctaatgtac cgaaaccagt gacagagatg ttctctacat tttgagcata
     4801 gatacaggaa gcataaactt ttcgatggac tccttcccaa cgtgaatgaa caacaggata
     4861 atcttttgga tcgtcagaga atttcagaac cgctcctgcg cttaaatgaa gttcgacatt
     4921 gctttttaaa aataaagctc ctgttagaaa ttcacctgct ggaacaacta cggtcccgcc
     4981 tccgtcactt gctgcagcat caatggcttg ttgtattgct tcagtattca attcatcaat
     5041 tgatgcgcca aatgtcaaaa tatcatacat atttataatg cctccacttc aatcaatgct
     5101 tgcaagaatg caccaacgcc tttttgatca ttacaaataa ttggttcact gatgtagtaa
     5161 gcatatgaac catctcgctg atctgctccg cctaaccctg ctacttgaca gtttttattc
     5221 aagttcagcc agccttcttt tgtttccaga acaaattcat caagtaatcc ttgatgtgat
     5281 ttcttcactt gtggaagcca ttcttctttg ttcagcagtc ccaaacgtat tcctttagcc
     5341 attgcgtaaa caaacatact actgccggaa gcttccagat aatttccttt ccgttttgtt
     5401 tctgttgtta cttgatacca tgtcccgctg ttttcttctt gatatggaag taatgcagcc
     5461 agtgtctttt ctagaatttc tttcagcaaa gaacaatctg tttcttcatg aagtaattca
     5521 taggtatctg ctgcagccat caaataccag ccaatcgatc ggctccaaaa atgatgggat
     5581 agacctgttt ttggatcagc ccatggttgg accttctttt catcccatgc atgaatcagc
     5641 aaatttgttt ttggatcgaa tagatgttta taactgattt caaactgtct caaaacatct
     5701 tggtaatctt tttcttcact aaaattctgg ataaattcag cataaaaagg agaacccata
     5761 tataagccat ccaaccaaat ttgataagga taaatttctt tatgccaaaa agctccttcg
     5821 gatgtgcgtg gatggtttgc caactgctgg gaaaggagtt ctgctgcttt tttatatttt
     5881 tcttcgcctg tttctttgta caataaaaag aaaagctttc ctgtatttac atggtcgata
     5941 ttgtattctt caacgctgta gccacggatc gttccatctt cttgcacgaa atagtcgata
     6001 ttgtctttta tgaaatcaaa ataaattttt tcttgggttc gttcccaaag taattggaac
     6061 cctttaagta cgacgccata gtcataagac cattttcctt tataccactt ttcttcatat
     6121 aagcgaggtg ttctttccat gatacttttt gctaatttct ttgaccagtt ttccattatt
     6181 tgctctcctt taaaaattaa aataggattt gggacaaata ccaagaagta tttgtcccaa
     6241 tcgtctatat tactgtgccg ctgctttgta tgcttcctcg tactcttgtg tgattcgatc
     6301 gcctccttgt gctttccagt cttctacagc ttttttgaat ccatcttcgt caagctgtcc
     6361 catgatgtat tgcaccgtag catcaatgac gattttttca atttctgatc ctacttcatt
     6421 gttcgtcggt gaatccaatg gcacagaagg atccattaca gcaaattctt cattttcttt
     6481 gatcaattga ttagagaatt ctttttcagg gtctgtatct ttgaaagaga acgtcacttc
     6541 actaggacga gagcttgata acggttgcac ctcttgttgc caaagatcca tatcgataaa
     6601 ttcgatagaa ccatcatcat tcaattcata atgcgttcct tcgataccgc cagtcatcaa
     6661 tttatacaca tcttcttcta aaagatcatc aacaaattga agtaatttct tcaattgtgc
     6721 ttcgtctttt acttgagatt tgggaaatgc tagcaagccg ccaataccat ttccttctga
     6781 ccaaacatga taaccgccgc cttcaccatc tgagatcttg tttacaggca ctaattccat
     6841 atcatcttga ataccttgac ccatcgtctt cagattcttg atatccacca ttccagtgta
     6901 gattccagtt cttccttgtg caaattgttc ttgctgatca gtcttttggg taactgcaaa
     6961 gtcttgtgcc aaatagccat tctcaaatag atctcttgaa aaattcaaag ctttcatata
     7021 ctcttctgta tcaaattctg gagtgaattt gccattatcg tctacagccc agccatttgg
     7081 tgctccaaaa taagaagtga aaagtttaaa gctggtataa cgaatatcac taggcgcacg
     7141 atcgccaaaa ccaactgtgt cgtccacacc atttccatca ggatcgtttt ctgtaaattg
     7201 tttagctact tcataaagct catccatggt agttggaaca tctagtccta gattatccaa
     7261 ccagtctttt cgaatgacta agccagcacg agcgtagtct ttttgtatcg gtacgccgta
     7321 caaggcaccg tcgatactcg cagcttcgat tctatcttca gaaatttgac ttaaattttt
     7381 atagtctttt aaatattctg atacatccca gaatacgcct gattttaacg cattgcggac
     7441 agaagagttt ttcaacatcg caatttgaag tgtaacgata tctcctagtt catcagaagc
     7501 caatgcagtt gttaatcttt cttcttttga agcatcagga atccagttga atgtcagttc
     7561 cgtatcggtt ttttcttcga tcaagttcac aatcgtgtct gtaggcggtg aagcatgatg
     7621 caaaatattt aaccaagtga ttttagcttt tccatcttct gtctctgaag aactgttact
     7681 tgaactgctg ccgcatgcgg caagtgctag aattcctgca aaactaagac ctaattttac
     7741 gagcgttgct tttttcataa attttctcct tcactgtttt aataatattt ttgttttctt
     7801 ctgttttttc tgtatatgag ttcttccatt ccaaatggca aaatatagaa tttgctgtcc
     7861 acgtgactgc ggaaaagaag agactgccac cgataaaggt taaaacacct ggcattgttt
     7921 ggatgatcat cagataaatc cctaccatac ttaatatcaa cactaatgag taatgaagat
     7981 gtgagaatcc gaataaaaat gccattttca ttttgaaaaa atatcctttc caatcataat
     8041 gagccatgat tgggaaaata tagagtgctg agagcacata tatgaacgca gcaatataca
     8101 aagcgatcct aagataccaa ttgtgtatga aagaaagatc cacaagtaaa acaatcccga
     8161 tggcgccata tatccagctc atcagcaagg tttcacgata gttttcttta aaatatgtga
     8221 agtacgtttt cgtcaaagga aacgtttctt ttgagcgcag ccattttcta atcactgcaa
     8281 ccatcgcata cgatgcagga ccgaaagtaa aaactcccaa acctgcgatg actaaaccgc
     8341 cccacagaaa attcaaataa accaactgta aaatacgatt tagccttccg tttattttat
     8401 ctaatgactg cagcatatct ttccctctct ttctatctta gtaaacgccg tcttctccta
     8461 gctttttggc aatcttatta gctaaaatta ccataaacaa tcctacgacc cctttaaaca
     8521 atcccattgc tgtactgaaa cttaactgtc cattcttcaa tccagctgta taaatatagg
     8581 tgtcgaaaat ttctgcaacg cttcgattca atgaattcaa taacaagtac atatgctcga
     8641 accctaagtc taatgtctga ccgattttta gaatcagcaa ggtaatgatt actggacgaa
     8701 ttgccggcaa tgtaacatgc catgttttgc gtaagcgtcc tgctccatcc atttctgcag
     8761 cttcatacaa ctgtgtatcg actgcagtta tcgctgaaag gtagatgatc gtagaccagc
     8821 ccaactcttt ccagatcact tgtccgatat atactgtgcg tgtccattca ggggatgtta
     8881 gaaaactgat tttttcaaat cccattgttg caaggaattc attcaatacc cctccgtcaa
     8941 cattcaaaaa tacataagta atcgaaacaa tgattaccca agacatgaag tgagggatgt
     9001 aaatgatcgt ttgaatcccg tttttcaatc gtttgttcgt tacttcattt aatagtaaag
     9061 acaaaatgat tggcatcgga aaaaacacga agatatttaa tccgaacaag atcaacgtat
     9121 ttcttaataa tgtaaagaat gtcggttctg taaacaaacg aatgaaatgt ttgaaaccga
     9181 cccaaggact tcccattatt cctaaaaatg gttgataatc ttgaaaagca ataacgagtc
     9241 cccccatagg aagataccta aaaatcaaga aataaaggac tcccggcaga atcatcacat
     9301 ataaaaattt attcgttttg attcctgcaa ttattttttt tcgtcttctg agcttttctt
     9361 cttttttcga tagcgtcagt tgaaatggtt cttgtttctt tacactttcc ataagcccct
     9421 ccttagtttt gtgaatgttt gctgcttatg acttcatgat agataactat ctgaattacg
     9481 tatataatca tttttaccgt aagtaaaaac gcttacatag caatgatttt tgtgctatac
     9541 aaaacgataa agtgtatttt ttcacaaata aactacgcat agcaaacctt ctattttttt
     9601 ctatctgaca cttagcatag gaaatgatta tatacgtaat ctttcaccga tgataaattg
     9661 aacatataga aacagaaaca tttaatttat tttgaggagg agcctatcta tatgaaaaag
     9721 aaagtctcta taggagaaag aagttttaca cttttcaaca atacgtttct aattttgcta
     9781 gctttgatct gtatcgttcc ttttttaaat atcatcgcta catcttttgc ttctacacaa
     9841 gaagtcgtcg cgaaaaagtt tatccttttt cctactacct tttctttaga cgcgtatcgc
     9901 tacattcttt ctacgccgac catttttaga gcacttgctg tttcgattgg tgttacaggt
     9961 gtcggaacga tcgtcagtat gtgtgcaact tcactgatgg cctacggtct ttccagaaag
    10021 tatttgtttg gtcgaggttt cgtcaacttt cttgttgtat tttctatgtt gttcagcggg
    10081 gggatgattc caacgttctt agttgttcgt tctctagggc tggtcaattc ttactgggcc
    10141 atgatcctgc ctgtggctgt taatgcaatg aatatgatca ttatgagaaa ctttttccaa
    10201 gccttgccgg atagtttgga agaatcagca aaaatggatg gttgcacaga ttttggtgta
    10261 tttttcaaaa ttatgctgcc attagcgtta ccgtctattg ctactatctc gcttttttat
    10321 gctgttactt actggaacac ttatatgact gctattttgt atatcaacga ttcatctaaa
    10381 tggccgatac agattctttt acgccaaatc gtcattgtat caagtgggat gcaagcagaa
    10441 agtagtgccg ttgatgtgat tccaccagca caaacagtta aaatggctgt tatcgttatt
    10501 gcaacagtgc ctatgctgat tgtttatcct tttgttcaga aatattttgt caaaggagca
    10561 ctcgtaggtt ctgtcaaagg gtgattatgt actgaacgca aaatggtagt tgtgtcagaa
    10621 gtggtcagct tcaagaaata agaaggcatt tacgaaaatt gcttcttaaa tttttgtgaa
    10681 atttcggctt atttttcgaa gaatctactc ctggaacacc gtgtatcaga ggttgtgaaa
    10741 gagacgttca gccttgagcg atgataaaaa ctctcacaaa cattgcgacg agtaacccac
    10801 aggagcaata agaagcgatt ttgaaaaata gcttcccata tttcacaaaa ttatgcctta
    10861 ttgtcgatag cctgtctctt gaacgccatt tattcgtaaa aaagaactgt gacaagactt
    10921 ttgtcacagt tctttttgcg tgcttagcct tgaaatttaa ctatccataa atacaaatac
    10981 tagggttatt ctaacttctt ttattgcttg aatcagtcca caagaaaaac agcctcttga
    11041 ttcacaaata tttttttcga tattctcccg gcgtaatcga ttccttcttt ttaaagaaac
    11101 gaataaaatt ttgcggattt cgatattgca atctttctgc aatctctttt acggagaggt
    11161 tagtctcttt caaccatttt tttgctacct ctaaacgatg attttgaaca aaatcaccaa
    11221 atttttctcc tgtttccttt ttaaatacat tacttaaata gttagggtta taatgcaacc
    11281 tatcagcgat cacatctaaa gacagatcac gatcatattc tgtgtagacg atatgcataa
    11341 tattctccga caaattttta aattcatgat cagtgattgc ttgagagcat tctgtgattg
    11401 gcagaactaa ttgttctaaa acaagttttt ctagttcttt gggatgataa ttgttcagtg
    11461 cttttacata aatttgtttc atgttatcaa agttttttga atctactccc aataactgtc
    11521 ctaattgaac gagttcattg atcaagcgga ccgaaactac ttcccgactc aaaggatttt
    11581 tattttgtgc aaacaatgca tcaatcaatt catgtactaa attcttcact tcctgatttc
    11641 cagaacgaat ggcttcaaac aatcgatttt gttcttctat cggatatcta ataagtgttt
    11701 tttcatgagc agcaggaacg atttcctgat aaaaaataat tgaacttgga ccagtattta
    11761 cacgataata cagggcttct tttgcccgat caaccgattg cttgctttca ttcagcgttt
    11821 cgaaccggtt actgatgcct atactgatag ttaaattcaa gtattctttt acacgttttt
    11881 gaatgagtcg gcaatactcc atgacttttt tgcttgcttc tgcatcatct ttatccattc
    11941 gataaatagt ggcttgcatt tcttcattca aaacgatcgg cagcattcga ttttcctttg
    12001 gcacaatttc cgtgatcatg ttgttgatcg ctaaaagtag caaatcacgc tccccaccgt
    12061 gactatcttc taacagatcg atttgaacca aagctgtata atagcagtca ttttgagaac
    12121 tataaccaaa ttgctgcaac cgctgatcta gttctcgcct ttctactcga tttctaaata
    12181 aactaagtac aaatagcgtt tccagctgtg gcttttgggt aactaagtgt gcacttaacg
    12241 cttctttttc tcctatgatc cgactgactg attgagcgac caaatctagt tctttcccac
    12301 tgaatccttt tgatttaaga ttcagacgtt cctgtatttg agaaatcgga cgggcaaatc
    12361 tgtccgaaaa taagtaggaa agaagaccaa tgagtcctac cagtgttaaa gacactgcaa
    12421 ctagcccgat tctcagattg cggatcattg cgccaattgc actagagtca acttctaatc
    12481 tgtaaatcca attattataa tctgatttca gtactatata cgttttatct ttttctctta
    12541 tcactttcag ctgattgtaa tctagatcca cgtccgttaa tacagttggt tgctctttat
    12601 tttttactgt ctgagtagta aaaaggttgc caacttcgtt ttcaatagaa agtatgtctt
    12661 ctccactttc accaacaact tgttctattg tatgtttctc tatcgtagca attcccaatg
    12721 catatttttc tttttcaaac attggcaaag aaatcaccat ttcgattcct ttgtttttct
    12781 tttgccaaaa taaattattt ttgttattta tgtacttctg ttgatacttt tcaacttcct
    12841 cttcggtcag ttgactcaac gatccattga ttactcccca tttaccagcc aaactgacta
    12901 aatcataatt attatcatcc ataacaatcg tttctataaa atttagttct ttcctgattt
    12961 cagaataagt ggagtaatct gtatatgtca gcggatgctt gaatatgtcc gagaaatttt
    13021 tggtcgaacc atacgtagaa aacgaatatt caatcgttct aattttccat tcaacgttag
    13081 ttttaacttg cctgagcaaa ttctttttgt ctgttaaata actcgattcg atcgattcat
    13141 aggtattctg gtaaatgaat aaactaaatc ctatgacaag tacggctcct aataaaaaca
    13201 tcggtaaaaa tattttataa aatactgtac ttttcttcat tttcttcctc tcctcctttt
    13261 aaaataaaag gctttcattt ttctgtaaaa ttgatactca tctaattaaa tgagcattcc
    13321 ccgctttcaa gaatagcata attaaaaata aaaatccaag taatttttaa cgtgtgcaat
    13381 cttttcatat caaagatcta ttgacttatc gtagttatcg taatatgatg aatgcgttaa
    13441 caaaaagtga aaaagtaagg aaaatgatgg aatggtaacg atcaaagata tcgctaaagc
    13501 agctggcgtt tctcatacga cagtttctcg tgctttgaat aacagcagtt taataaaaaa
    13561 tgaaacaaaa caaaaaatac gacaactagc taaggaaatg aattattcgc caaactatag
    13621 tgcaaaaggt ttagtgaatc aaaaaaaata tctaatcgga ctatttttct caagtttgca
    13681 taaaggaact tcatcaaatt tcctagcaga ttccattgca ggcatccatt cagtattaga
    13741 cacaaattac agtttatctg ttgaaagtat cgatgttgtt gatttaacag aaatcaacct
    13801 gcaacgctac gatggactga tcgttatgag tcaaagtgat gatgatcagc ctttcatcga
    13861 ctatctcaaa gaaaaagaat ttcctttcgt tgttgtcaat cgtcacatca atgatccagc
    13921 ggttatcaac gtcactgccg atgatgcggc cggtgttatg caggcagtag attacggtat
    13981 tggatgcgga catacgaaga tcgcttatat tggcggaaag aaaaattttc gttcgacaaa
    14041 agaacgggaa aaaggggtat tgaaaagtct aaaaaaagcg agcatccctg tcaatccagc
    14101 ctatttttta acaggagatt acagcttaga aagcggcctc caaaacatgg aatacctatt
    14161 atcactttct gacctgccta ctctggtttt ttgcggcaac gacgacatag cgattggtgc
    14221 attgagagct gtacatttag ctggcctccg tgttccccag aacatttctc tgatcgggtt
    14281 cgatgattct ccagtcgtct cctatctcaa tccgccgctt accaccgtac gtaagccttt
    14341 gaaagaaatg tgtcaagaag gaacaaagct tttactagat ttgatcgaag gcaggacgat
    14401 cgaacaatct aaaattgagc tctccacctt gttgatcctt cgtgagtcga tcaattactt
    14461 gtaaactaca agtaatttta tataaaaatg ttaacgtgtg cataacacag aaaggaagtt
    14521 tatatgaaaa aactgacaaa agaattagtg aaaaaaaacg aagcacctgt aaaagttctt
    14581 cagtttggtg aaggcaattt tatgcgtgga tttattgatt ggcagattca gcagctgaat
    14641 aaccaacaat tgtttaaagg taatgttgca atcattcaac ctttagaaca aggtcttgga
    14701 aatatgatga accagcaaga taatttatat acggtcattt tacaaggact cagcaatggc
    14761 gaaatcgttg atacttccga gattatcacc gtagtagaac aaatgatcaa tccttatgat
    14821 gaatgggatg cttatctatc tctagcagaa aatgacgact tggcctatat cgtctccaat
    14881 acaacagagg caggaatcca attcgtggag actgataatt taacagatac tcccccacag
    14941 agcttcccgg gcaaactgac agccttttta taccgacgtt tcaaattaaa caaagctggc
    15001 tttaccatca ttccttgcga actgattgac agaaacggcg aacgtctaaa agaaatcatt
    15061 ttacgctacg cagaaaaatg ggatttagaa cctgcttttg ttgagtggtt agaaaaagaa
    15121 aatgttttct gttgtagttt agttgatcgc atcgtcccag gttatcctcg cgaagatgca
    15181 ggtgttttca atcaacggca taactatgaa gatcaattga tggtcaaagc agaaccattc
    15241 atgctttggg tgatcgaagg gcctaaagaa ttggagaatc aactaccatt caaacaggct
    15301 ggcttgaatg tcatcatcac agatgatatg actccttatc gtgaacgtaa ggtgcatttg
    15361 cttaatggac cgcatacagc gatggtccct cttgctttgt tggcggaatt ggaaactgta
    15421 gaagaagtaa tgaaagatcc tttattctcc gcatatgttg atcagttatt caatctagaa
    15481 ttgattccta tgctttcttt acctaaagat gaattagcca tttatgctga ccaaatcaaa
    15541 gaacgtttct tgaatccttt tgcccatcac aaattagaag cgatttcatt aaatagtgtt
    15601 tcaaagttca gcacccgtct gcttccagtg ttcaagaagt atatcgaaga acaaaatcag
    15661 gtaccaccgt taatcactgt ttcactagct gcgctacttc ttatgtatcg aggcgatcaa
    15721 gtaaagccac atgatgatga aaagacgata agcgaattta ctgatgcttg gtctgataat
    15781 ggaacagcta ttcctcgatt attgcaaaac gctgcgcttt ggggagaaga tttgtctcaa
    15841 ataccgaatg tgacagatac agttcaagaa atagctgcaa agatcgatca aaaaggtgtc
    15901 cggtcagtat tacaagaaat gaaaggaacg atggtccatg cttgaaaccc tgcaaccaac
    15961 aatgaaatta aatcgacgag acagtgtggt cgtcgcatta acccctattc caaaacaaac
    16021 acaactgacg attgatcagc aaacgatcac cactcttgaa gatatcccgc aaggtcataa
    16081 gattgcattg ggtgatttga aagctggaga caacgtgatc aaatatggct atccaatcgg
    16141 tcatgtgacg acagacgtaa ctgccggtca atggctgcac actcataatg taaaaaccaa
    16201 tctttctggt gaattggatt atcgatatga aaaagatgtc catccaagtc attacctttt
    16261 tgagaaccga acatttcaag gttacttgcg aaaaaatgga aaagtaggga ttcgtaatga
    16321 tttgttttta gtacctaccg ttgggtgtgt gaatggcatt gctgaactga tcgtcaaaca
    16381 gttcaaggaa aaacatccag atctgggaca gtttgaccat attacgattt taaagcatcc
    16441 ttatggctgt tcgcagcttg gaaaagatca tcaaaatact cgagaaattt tagcggacgc
    16501 agtaaatcat cccaatgccg gaggagtttt agtatttgga ttaggttgtg agaacaatac
    16561 agttcctgaa ttcaaaaaaa ttttaggtga atatgatgaa gaacgagtga aatttctagt
    16621 ggctcaagat gtttacgatg agatcgaaca aggtgtggct ctattagaag agcttttaac
    16681 agcggcggaa aatgaccagc gcatatcagt cccgctctct aagttgaatg tcggtttgaa
    16741 atgcggcggt tctgatggtc tatcgggaat aactgctaat cctttacttg gtgcattttc
    16801 tgactacttg attgctcaag gaggcagcac gatcttaaca gaagtgccag aaatgtttgg
    16861 cgcagaacaa gtattgatgg cacgagcgga aaatgaagaa gtttttgact caatcgttga
    16921 cttgatcaat gacttcaaac agtatttctt gtcttacggg gaaccagttt acgaaaaccc
    16981 atctcctggt aataaagcag gtgggatcac tacattagaa gataagtctt taggctgcac
    17041 gcaaaaatca ggaagctctc ctgtcgttga tgtgctacaa tatggcgaaa aaatcagaaa
    17101 accagggctt agtttattgc aagctcccgg gaatgatctt gtagctgcct ctgctcttgc
    17161 ttcatccgat tgccagttgg tcttgtttac cacaggaaga gggactccgt ttggctcata
    17221 tgtcccaacg gtcaaggtat ctacgaatac aactcttttt gaacgcaaag gtcattggat
    17281 ggattttaat gcaggcgtct tattagaaca accaatggaa gtaatcttag aatcgtttgt
    17341 tcaaaaaatc atcgctgtag ctagtggaga agaaacgaaa aatgaacaaa atgacgttcg
    17401 agaaattgcc atttttaaaa acggagtaac actttgattt tcataaaaca gtataaaaag
    17461 aaagaaggaa gatttatgtt tttatctgat gattttttat taaaagacga ttgggcgaaa
    17521 aagctttacc attcctacgc aaaaaacatg cccattattg attatcactg tcacttagat
    17581 ccaaaagaaa tttacgaaaa caacaatttc agcaatctga cagaagcttg gttaggaggc
    17641 gatcattaca aatggcgctt gatgcgagct tgcggcgtgc ctgaagaaaa aatcacagga
    17701 aatgcttctg attttgaaaa atttttagct tggtgtcaaa ccgtaccaaa aattatcgga
    17761 aatccgcttt acagttggtc acatcttgaa ttgaagcgtt tcttcggtat tgacttgttg
    17821 ataaatgaag aaaatgcaga gaaaatttgg acacaagcaa atgaaatttt ggcagctcct
    17881 gattttcgcc gtcgggaaat cgcaaaaaaa tcgaatgtag aagtgatttg tacaacagat
    17941 gatcccgcag acgacttgca ctatcatcag ttgcttgctc atgaagaacc agatttgaag
    18001 gttctacctt cattccgtcc agataaagca ctcaatattg aaaaagaagg atttaatgca
    18061 tgggtcacac tactggaaga agcagctgac caaacgatca ccacctatca agaactgata
    18121 gaagtcttag ggcagcggat agaatacttc catcaacatg gatgtcgtgt ttccgaccac
    18181 gctttagaca tcttacgcta taaagaagcg gatgaaacag tattagaaga gatttttcaa
    18241 aaagcacgag ctaacaaact attatcttca gatgaaatcg atgcttatcg tacagaaaca
    18301 ctgatacatt tgacccactt ctatcattct cataattgga cgatgcagct gcacatccat
    18361 gcttatcgta actgtaatac acaaatgttg gaaaaattag ggcccgatac cggctatgat
    18421 ggaatgaatg atctttcttt gactattcct ctgcaaaaat tattgaaccg tgcagaagaa
    18481 acggatcagt tgccaaagac gatcctttat tcattgaatc cgaatgacta tcctgcgatt
    18541 ttagctttga tgggctgttt ccaaaaagaa cagaatggaa aactgcagtt aggttctgga
    18601 tggtggtaca acgatactcg agctggtatg cgcgatcaat tgacacaatt tgctgatggt
    18661 agtgctttgg ggaattttgt cggcatgctg acagattcaa gaagtttcct gtcctacact
    18721 agacatgaat atttccgtcg tgttctttgt gaatttttag gcgaaatggt catgcgagga
    18781 gaagcacctg aagatgaaca acttttagga agtctagttc aagcgattag ttatacaaat
    18841 gctaaaaatt actttggctt ttttgcctaa ttgattaata gtaacagtaa cgaatcaaaa
    18901 acaggatagt gaaataacgg aatccgttta tccacgtttt agagaagaag acggaccaaa
    18961 tttgctccgt cttcttctct tgctgttctg cttcattgcc tataaacctt tatgaaagaa
    19021 gagataacat gcctactatc catattgccg gtgattctac tgcggcagaa aaagatgaaa
    19081 acaagcgtcc cgagacaggt tggggagaaa aaatctctgc ttatttttca cccgagatcc
    19141 agatcaataa ccaagcaaag aatggacgca gcaccaaatc atttatccag gaaggaaggt
    19201 tagcccgtct agcttcacag tttcagccga atgatttctt atttgtacaa tttggacata
    19261 acgaccagaa aatccaagaa gaaagaggta cgcttccctt cggtagctat ttaaaaaatc
    19321 tgcagatatt ttttgaatgc gcaaaaaaag cgaacgtcca aatggtctta ctgacatctg
    19381 tgacgcgacg agattactta gaaaacggaa cattgaatcc agatatactt ggagactatc
    19441 caaaagcgat gcgtgcattt gctgagaaac accatattcc tcttttagat gtgttctcaa
    19501 gatcacaaga attgttccag accttctcaa aagaagagac aaaaaaattc tacctgcatc
    19561 ttatacctga tacttgtaaa aattacccag aaggactaaa agacaatacc cactttagtc
    19621 cagaaggcgc tgataaagtt gcccgaataa ttgttgaatt gataaaagaa tcccgtctgc
    19681 ctcttgctaa ttatttacaa aaaggagttt gagatgtttc cattaaattt aggcatccgt
    19741 gcccacgatt tagatgttcg aaatcgtgaa gaaatcgttc aaaaagtatc acagcttcac
    19801 ttgtcccata tccaatttgc accacaaaaa gcatttcctg aacaacttaa attaactgac
    19861 atgacacttg ggagtgctgc ctattttggt gaatacttcc aaaaaaacaa tatcgaactt
    19921 tccattttag gatgctatat caacctatcc agcaaaaatc aagaagttcg tgcacaagca
    19981 ttggcaactt tcaaacagca aattgctctg tgctctgcct atcaagccaa gatgattgct
    20041 acggaaaccg gtagtgtctc tgtcggttac acaaaagaaa attttactga agaagcttat
    20101 cagatcgcca gaaattctat cattgaatta gtcgctcatg cagaaaattt tggcgtaact
    20161 gttgctgttg aagcagggat caatcatcca atctataact atcagttagc taaacgttta
    20221 atcgatgaag tagcttcacc aaatttaaaa atcattttag attgtgcgaa tttgatgcat
    20281 aaagaaaatc atgctgatca ggaaaaagtg atccgaggtg ccttggacaa tttacatggt
    20341 tatattacag cgcttcatct caaagactat atcatggaaa atggaaagat ccgcatggta
    20401 cctgttggca agggctgttt agatttccgc ccggtactgc attatatcaa atatgagcgt
    20461 cctctaatgt acgccactct tgaagctact cctgagattt atgttcctga agcaatagaa
    20521 cacttgcagt ttatttacca agattgttaa ggagaagatt atggaggaac gaatacagaa
    20581 aagttatcaa aaatatcatg caaatgcttt tcagacattg ctgcatcttt atcagggaaa
    20641 ttattggcgt tttgccatgt caggactctt ttttattatc aaacatcttc catcttggtt
    20701 aatgccgatc gctattgcca atgtaatcaa tgcagcttca tcgaaagatt cttctcagtt
    20761 gcaggccatt tatttcaacg cttttttgat ggcttgtctg attggtcaaa atatattgac
    20821 gaattatttt catgtaaaat accatagcac ttcgatccgt caagtagaag ctggactaag
    20881 agcgaccctt atcaagaaaa tccaagaact atctattcct tatcaaaaag aattgcaatc
    20941 tggacgaatc cagtcaaaga tcatccgtga tgtagaagct atccaaacgt tgtcctctca
    21001 agtatttgtc agcggattaa atatcgctgt caatttattc gtagctttag gtatcacgct
    21061 ttataaaagt cctgttgttt tcatgttttt cttattgact gttcctctcg ccgcattcgt
    21121 tactctcttt ttccgtaaaa ggatcagaca gtccaactca gacttcaggc aagaaatgga
    21181 agagacttct gcaaaagtga tcgaagtgat cgaattagca ccgattgctc gagctcatgg
    21241 gttagaaaaa cacgaaacaa ctcgcttaaa taagcatttt actcaaatct atgaaaaagg
    21301 attccactta gatatcctgc aatctgtttt cggatcagtc agctgggcta gttttcagat
    21361 atttcagctg atttgtttga ttttcactgg gatattagct ttacaagggc gcatccaacc
    21421 cggcgatgtc gtgatgtatc agacgtattt cacgacgatc gtcaattccg tttctggaat
    21481 gatcacgctg attcctacga tttcaaaagg catggaatct atcacttcta ttggcgaact
    21541 gttgattgct ggcgatgtag aaacttatca cggtaagaaa aaaatccaac atttggatgg
    21601 aaattttcgt tttgaggatg tttcttttct ttatccggac tcactcaaac cgattatcga
    21661 tcatttttct ctagaggtga aagctggaga aacaattgct tttgttgggc catctggctc
    21721 tggaaaatct acactcctta acttattgat tggatttatc cagccgactt caggaaaaat
    21781 ttttctagat gatcagcctt tggatgaact ggatatgcga agtgtgcgaa attatttagc
    21841 cgttgttcct caaaccactt tgttgttctc cgcttcgatc aaagagaaca tcacttatgg
    21901 cttgaaaaac gtatcaaaag aacgtttaga tgaagtcatc gaagctgcgc aactttcttc
    21961 actgattgac cagcttcctg atggtctgga taccttagta ggcgaacatg gcaacaagct
    22021 gtctggtggc caaaagcaac gtatctccat tgcacgagcg ttgatacgtc agccaaaaat
    22081 cattttgcta gatgaagcaa cctcagcttt agataaccag tcagagaaaa aaatccagca
    22141 agccctgaat tacctgacag aaacacccac tacttttatt gtggctcatc ggctttcaac
    22201 gatcaaagag gcagataaaa tcgttgtgat cgatgaagga aaaatcgttg aaataggtac
    22261 ttacgaaaaa ctgttagctc aaaaaggcta tttctaccgc ttgaaaaatg aatgacaaaa
    22321 aataggagct atgacaagac ttttgtcgta gctcctattt ctgaataaac ggtgttctcc
    22381 tgccaactcc tcggtcacaa gtcacaatag tcatcaaaca tgagaagctg ttttttgaaa
    22441 ttgttccttg taactcagag ttaaacggta ggctcacgac ctcttggtaa atggtgttca
    22501 agagacagcc tatcggcaat aagaagcaat tttgtcatcg ctcaaggccg aacgtctctt
    22561 tcacaccctc tgttgtttca taaaatagtt ttattcgctg taaaggtcgc gaactggtgt
    22621 catgatgata cggttcaagt cattgatgat tgtactgaag ccttgctctt tttgcatcaa
    22681 gtcattgatt acttcttctg attggatttt cacagccatt tcttgtgctt tcattgcatc
    22741 ttcgtcgctg aactcttgtc cactcatttg tttttcttgt aattcgattt gcatcgcttg
    22801 gaagtctttg aacagtttgt gtgcttcttc gtttgcttgt actttctcgt acgcttcttt
    22861 caacgctttg aattctggta attcacggat ctcacgttcg atttgattcg cgctatcgta
    22921 gatgtttgac atgttatgcc ctcttttctt ttagtagtta ctctctattg taacatgggg
    22981 ttattcgttt tcaaaggggc tactctcctt gatatttccg ccagaggtct ttagccgctt
    23041 caccaattcc ctgaacgcct tctttcacca tatcgccgaa gtttttcagc ccttcttttg
    23101 cttgttcacc atattgattc aagttatctt gccaatttcc agactctcca ttagaatttc
    23161 ctgtgtttcc aacttgatct gcagctacca cttgtcctcc tgtggcatag gcatctgcta
    23221 cactaaatcc tgtttttgaa gtatacggaa ggatgccttc tgccgctgat ttgaagactt
    23281 tcccgacaac atttccactt gtcccttcca aatagtgcag cttgctggat tcttcgtaac
    23341 ccatccatgt cgaaatcact acgtctggcg tgtaaccgat catccattgg tcatttgctt
    23401 ttgtggcatc gaaattagtt tctgtggttc ctgttttccc tgctatcgta taacctgcag
    23461 gattcgcagc agccgctgtt ccatttgaaa atgtaccaag aagcatgctg gtcatttcat
    23521 cggctgtttc ctttgatatg acctgttttg gttttttgtt tgtgttatcg acgatcaccg
    23581 ctccagtcga atcaacgatt ttcgtgataa gatgcggcgt atacatctga ccatcattgg
    23641 caaatgcact gtaagcaccc gccatgatca atggagatac tcctttttcc agtccaccaa
    23701 gggctaagcc ataatactta tcagattttg ctaaaggcag gccgaattct tctgctttat
    23761 catatccttt ttgtaaaccg atctcatgca aaagccatac agtaggcaaa ttcaaacttt
    23821 gtgcaacagc ttgatacatc ggtacttctc cttggtatgt cccgtcaaaa ttatgcgcat
    23881 cgtaataact ttgcggttcg tctttcaaga tactggaagg cttgtatcca gcttccagtg
    23941 ctggtgtata tacgcttaat ggtttgatcg ttgatcctgg tgaacgacgc atctgtgtag
    24001 caaagctgaa tcctctaaag acatgatctc ctcttcgtcc tactagtgct tgtacaccac
    24061 ctgtattcgg atctaaagct actgaagcag attggaccat ggcaccatct gaagcatttg
    24121 ctgggaataa tgcactgttt tgataagtag cttctaattg cttttgataa tcctgatcta
    24181 aagctgtata gattttatag cctttattca tgacatcttc ttcatcaaga ccatactcgt
    24241 taactgcttc atcgatcact gcatcaaaat aataaggata acgataacca gaatctgaat
    24301 tatcgtatgt gtcattaaga tacgcgctga tatctgtctg gctttctgct tgttcttgtt
    24361 cttttgtgat ttttccgttt tcttccatta cacttagtac tgtgtttttc cgatcattcg
    24421 cattttttgg gtgatcgatc ggattataaa taccaggacc tttcagcatt cctgccaaag
    24481 tagcagcctc agacagcgta acattcgttg catctactcc aaaatacttt ctggaagcgt
    24541 cttggacacc ccataccccg ttgccaaaat aggcattgtt aagatacatg gtcaagattt
    24601 cttctttgct gtacttcttt tcgatctcga ttgccaaaaa taattccttc gcttttcgat
    24661 caaacgtttg gtctaatgtc agatacgcat ttttggcaag ctgctgagtg atcgtacttc
    24721 ctccgccccc tgatgttccg ccagacaaga ccattcggac tgctgcacgg gcaatccctt
    24781 tgatatcaaa cccgtgatgc tcgtaaaaat tcctatcttc tgtcgagatt actgcatctt
    24841 ggatataggg agaaatcgcg tttaattcta catatgttcc tttttgggcg tacagtgtgc
    24901 cagcttcttc gtttttctta tcataaatga ctgtcgattg gcttaatcct gattttaacg
    24961 tttcgacatt tgcttgtttt gctaaaataa agaggtagat acttgtcact aagacacaga
    25021 tcaaaccaat cagcaaaatc aatttgttca catgatattt tttccagatg cgctttcgcc
    25081 atcgatggaa ttgtccgaga tatggtctga tccaacgcca aaaactgatc aaagcagctt
    25141 ttatcttttc taaaatgctt tgaaaatcca tttgcttctt ctcctgtcta tttgattcaa
    25201 tcgtcttatt ttaccatagt gaaagagtgt tttcttcctg cttaagttgt agttcaatat
    25261 gtttttttac gttagtgcaa gtagagtaac ataagaatga acagaaaaat gtaagttttt
    25321 tcttaaatat gttttaaaaa tggtacaatt agtctgcgat tgcactgaaa ggagtacact
    25381 tgtgaaaatc acgatcactt tacctatgaa ccatcctgaa acaacaattc gcgacttgct
    25441 tgaaaaggaa tggttagttc ctagaaaggt tcgacacttt ttgcgtatac gtaaaaacgt
    25501 ttggatcaat caacaacctg ctttgttcca tcatgaagta aagggcaatg accagatcac
    25561 attgttattc gaagaaacag attataatta tcaagaagtt caattaggag atgcgaagca
    25621 catccgtgtt ttatatgaag atgagcattt gattatagtg gataagcctg caggaatgaa
    25681 gactcatcca aatgaaccga cagaaaacag taccttactc aatcatttag ctgcttatct
    25741 cgacactaaa cagcaacgac cttacgttgt ccaccgcttg gacaaagaaa caagcggtgc
    25801 cgttttattt gcaaaaaatc catttgtctt gccgatactt ggaagaatgt tggaacaaaa
    25861 aatgatctat cggagatatc aagccatcgt atggggagaa attgccaaag atatgaccat
    25921 caccaataaa attggacgag atcgccatga tcggagaaaa agggtcgtag atgataaaaa
    25981 tggtcagagc gccttgacac atgtcactgt caacaaagta ctttctgaac aaacacaagt
    26041 ctattgtgtt ttggaaaccg gacgtacaca ccagatcaga gttcatttag ctcataaggg
    26101 acatccgatc gttggagacc ctctctacca aaatagaaca gctgaacgac tgatgctcca
    26161 tgcattagaa ctgcacatga tccatccatt tactcaagaa aaaatagtcg gtaaagcttt
    26221 acctggatta tgggaataga aataaaaaag aacagagtct tatcagcaga taatgataag
    26281 actctgttct ttttgtgtaa tttgtctgct tacttttgat gttcgtgttc tgctaacagc
    26341 aaactaccga taatcccact ttcattcggc agtccccaac gaacgatata ttcacttact
    26401 tgaggtgttt ccatataacc acccatcaat tcaacaaatt gctgacggat tttttgaagg
    26461 agatgatcct gattcattac cccaccgcct aaaataatct tttcaggtgc taacgtcaac
    26521 gtatagttga tcagtgcttg agcaatataa taagcctgaa tagcccaaac aggatgatct
    26581 tgcggcagat cttgcccttt gatccctgtg cgtgcctcaa tagacggtcc tgctgctaat
    26641 ccttctaaac aatctttatg ataaggacaa gtaccttcat aatggtcatc tggatggcgt
    26701 ttcacccaaa tatgtcccat ttcaggatgt gcgatccctg aaaaaatttc ttcattgatc
    26761 acgacacctg caccgattcc tgttcccacg gtaaggtaaa tacaactatt ttttccttgt
    26821 gctgctcctt gcttcaattc cccatatgca gcagcattga catccgttgt ccaagcagca
    26881 ggaatattgt agcgttcctt gattgtgcca agaaaatcga attgtgccca cccttttttg
    26941 ggagttgcca aaacgtatcc gtaattatct tttgttttgt cgataccaat cgggccgaat
    27001 gagccaatcc cgattccctt caattcataa cgatcaaaaa attcaaatac ctgatccaat
    27061 gtttcttctg gtgttgtggt agggatactt aatttttcta aaatttctcc accatctgaa
    27121 accgcacaca caaattttgt tccgcctgct tctattccgc catacattcg ttttcctcct
    27181 cgaatccgat tgtccgaatc tcataaggtt ggattattgg gacaaccgct tgcttcgttg
    27241 gttcttcaag cagattcaat atgacagttt ttccattgtc tttagttatt tccaattttt
    27301 ctaaatgact agacatattg aaacctctta ctactacttt atcactaaac ttgctgcgtt
    27361 tcaaagctgt aattgcaaac gtttcacttt ttatcgttaa atattgttgt tttgaaatca
    27421 atgttccttc atgatgtttg atttgttgag tggaaaaagg aatctgtgct gcgtatgcat
    27481 gcaaataagt agagaatttt tcttctggtc catgtagttc aatgctgtaa ttgaaccgat
    27541 gctcacctag acactgtgct tctggtgttg gaaaatatcc ccaatcgcct agttctccca
    27601 cactgcgaag tagcgtcact gcgatttgtc cgtcttctgt tacttcatat tcattcaaac
    27661 caaagtttcc gactgttaca ccgtactctt catcatgaat attgacaaat gcttgctgat
    27721 gttgcggatt cgtggggttt tcccaacttt tagacacatg gtttgggcgc ttcaccactt
    27781 caaaaatact atctgcttcg tgatgttcca catgtaactt tgtaggaaac aagacacgta
    27841 agcgatgatc tttcatctga ttgtcgagag tcgtttcaaa gtcgattttt ttactatctt
    27901 ttctgattgt catgattgtt tttatttgaa ggattcgttt ttctttggat cgttctgctt
    27961 ttcgataacg aaattccaca accatctgct gttctttttc taaacgctcg tctgcagaaa
    28021 cagggatttc catttcctgt agaatactta tttttgctat ttccggtgta tctactaata
    28081 cttttacttc agagttttct ttgttactgg ataggattgg ctgatcacag aaaggttgtt
    28141 taaagatata ctcatttcct atatcgccga tatcttcaaa aatatgcaga ttattcaact
    28201 ttcgtttgtt tttgcgatct tccattgtaa tggtaccatt tttttcaatc gtcaaatgta
    28261 gagggccgtt ttcgatgatt cgtccactct gatgaatcat tgtttccttt tcttctgctt
    28321 tcgcttcccc ttctacaagc gcgaatgttt cccaagaaaa tgctggcatg ttttctaatg
    28381 gtaaagtcac tttgatcatt cttgccatat atggcacacg gaaacggtct ttcggcaaat
    28441 cataaccaaa ttggacagtt tcttcagata aaatagctgg tatagtggcc ccactttttg
    28501 tttctacatg atacttacgt ttaggcaaat tttctaactc ttgatatagt tgttcaggta
    28561 ttccttcagc gaatcttttt ctttctaatt cgatggttat ttctgcttca ccagttttat
    28621 ccattcctgc agtatttaca atgacaaatg gaaagctgtc ttttgggaat ccggtcgtat
    28681 cgattgctgc ggtcagctgt tctaaggaat cttgtgcaag atactttcca acttcatctg
    28741 ctttttcaaa acgaggtatc atttcccgat ggacagaatc gactgaacaa ccgcaaatac
    28801 tgtcgtgagg atgattttgc attaatgtct tccaagcata atctaattga tcatggggat
    28861 agtttccact aacttcataa gccatcgtcg caagtggttc tgtaatattt tctagctgtc
    28921 gctggacctt ggtattccat tgttttaagt aaacccgagc agatgctgta ttggctaaag
    28981 tataccagcc atccgtttct tgacttgtca attctccttc tactgagcct aagtcttctg
    29041 gcacatcttt ttgaactgct tccagatagt cagtaaaatt agaatgaata aactcgtaat
    29101 ctggaaacag ttcatttgcg agatggattg cttttgatat atctttttgt accggttgat
    29161 gatcaacacc attcatcatc aataagtggt tcgtagaggc atattgttct gcatccgcta
    29221 atttttgctt ccaaaaggca agagctgctt ctttttctac tggaatctca ttcccgttgc
    29281 tataccaatt ggcaaatagc agaccaaaga ttgctgtttg atcaggtcct ttccaccaca
    29341 tctctgagta ttgggaagaa tagttttctg cttccaaaac ttgattatca aagccaattg
    29401 gttttacacc acggccaaac gctgctgctg aaataccagc ttgtttcatt aattgaggcg
    29461 tctgtcccat atttccaaat gtatcgggaa aatagcctaa catgacaggg gttccccatt
    29521 tacggctttc ttccatgcca atcagcatat ttctcacgtt cgattccgag ctgataagaa
    29581 aatcatcttg caagatataa aatggtccaa ttctcaattt tccttcattg attgcttgtt
    29641 ggattgcctg tcttttttct ggccttactt gaagatagtc atctaaaata atcgtttgcc
    29701 catccaaatg aaaactgttg aaggacggat caacttcaaa tagctctagt agatcgtcta
    29761 tcaattcaat cagtcgcatg tgatgctgtt cgtaagccat gtaccattca cgatcccagt
    29821 ggctatgaga aatgatatat actttctttt tcatttacga tacctcctac ttttctactc
    29881 taatatcaaa gtaatccatg actaattcgc aaaacatcat atttgcccaa gaaaaccatt
    29941 ctctcgtata attttcagga ttgttcacat cgaatccttc gtgcataagg tgtgttcctg
    30001 cgtcagttgc tacaagtaaa ttcaatatcc gctctttttc agatttgtct gacgttgtca
    30061 ttccttccat agctaatgca atcggccaaa cgtatttttc tggcgtatgg gaggatccaa
    30121 ttccttttgc gtactttcct tcatagaaat atggattttc tttactcaac aatgttcttc
    30181 ttgtttgcag ataatcttcc tcttgttcag aacaatatcc caagtaaggt gcggcgatca
    30241 gattgggcac attactgtca tccatgactg tggcgtttcc cattccatct acttcatatg
    30301 cataaatcgt ttgccctttt tgattaaccg ttcgtccgtg attctgaatc ccctcttgga
    30361 tctctttctt caatttctgt gtttgctgta caatttcttt atcatcgagt atcgtttgaa
    30421 aaatttcttc cacataacct aacacgacca ccgcaaacat attggatggt accagatatc
    30481 cataacggca agtatcatcg ctaggacgga aaccagacca tgtcattcct gtaaaagctg
    30541 tttttgcacc tcgcccgtca ttgatcagcg tatcttcttt tcttgtcgtg tctcgtacaa
    30601 atgtataagg agagtgttca tgatcttgct ctgttttgaa cacgtccaag atttttttga
    30661 taccttcaat aaaagattga ttgaattgat cagtttttcc tgtatttttg taaagaagat
    30721 aggccaactg tattgggtag cataaagaat caatttcata ttttctttcc cagatccaat
    30781 cgttcatttt tgtatgatct gtttggtggc cagcaccgtt agcttcttcg ttgaaggcat
    30841 tcgcatatgg atcgatattg atataaaaaa attgcttttt gactaacccg ctgatcatgt
    30901 cagctaattc ctcatcttct tttgcaatca ctaaataagg acgaacttgt gcagtagagt
    30961 ctcttaacca catcgctgga atgtctcccg tcaataaaaa ggtggttccg tcattttgct
    31021 ttttgactgt tgtcaacaga gtattggcaa atgctgctgt aaaattcttt gcccagtccg
    31081 catggatatc cccacatttt tgtgtgatat cttccataaa tttctgcacc gattttggta
    31141 tatctttata agccactaat aaaaccttct ttctatttga tatatcttta aagattatac
    31201 ttcatacagc caattatgtc tacgctttca aaaaatatct ttttatttac tttttcatcc
    31261 attatggtat tatttgaata tgatatatca ttaagaggtg tttatatgaa tcaaccattg
    31321 taccagacaa tatttaatga tttgcaaacg gctattttaa ctggtgaatt acctgtagac
    31381 agccaactac caactgaaaa agaactttca cgtacctata aagtaagcag aatcacttcc
    31441 aaacgtgcat tgactgaatt agagcaatta ggacttattt atcgcgttcg cgggaaaggc
    31501 agtttcgtta aatccgcttc tccggaacag attcttgctt ctcctacaaa aacacgacgg
    31561 attctgtttc ttcttccata tgtcgctgat ttgtctgttg gtgattttag caagggattg
    31621 aaccctgtga tgcaagataa acagattgat gtcatgatga cgacgcttga ctttctacaa
    31681 aataaaacag caaaagagct aatcaatgaa tttgacggat tgatttatta tgctcttgat
    31741 acagaacaac atcttgactt actttttgag ctttcattga aagaattccc tgtcgtcata
    31801 ttagataaaa aaatctatga gttacctttt cctaccgttc tttccgataa tttccaaggc
    31861 ggtgcgcttg ctgcctctta tttgatcgaa cagggacatt caaaaatcgc ttatttgttt
    31921 ggtacgcaga ctcatccaca atccgttcga cagcgttacc ttggttacct tgaagcattg
    31981 gacaaagctg gtttgacttt ccacacttca ctggatgaca aaagcgcgac attagatgaa
    32041 ttgctttctt atatcactaa gtatgaagta actgcttttg tttgtgaaaa cgatgttgtc
    32101 gctatccaga ccatgaatct cttgagacag catggttatc atatccctga agatttttca
    32161 attatcggat ttgatgatat ccaagcagca gctcttgttg acccgccact gacaaccatc
    32221 gcacaagatt ttgaacagtt aggtaagctt gcgggagatg ctttggtcac ttggttagag
    32281 acaaaagatc gaccatccga tagtaaattt ccagtttcat tcatcaaaag acaatcaaca
    32341 aaggagaaat aaaatgaata tcggtcaaat agatactaga catgcaacag cgaatcagcc
    32401 aaatttctct aatgggaact gccagcctta tactggcgtt ccttttggga tgaattattt
    32461 tgcaccgcaa actacggatc aaaacggcag ctggtggttt catcctgatg accgcgtttt
    32521 tcaaggctac cgtcttaccc atcaaccaag tccttggatg ggtgatttca gtcatatgct
    32581 gtttacgccg atcaatggaa aactacagga gaacaccctt ttccatgctc aaagttctta
    32641 ccgcccagaa gaaagtatat tttgcccgac tcatctgtcg atctgtcaat tacgttatgg
    32701 catctgttct acattgatac ctagcatgta tggcggaatc ttatctattg attacagcaa
    32761 aaatgacagc ggtttacttt tatcctttcc gggacgccat cagctctttg ttatagatcc
    32821 ttacacagtg gaaggaaaaa tcacacattt tgctggttca gaagatcctg atttttcttt
    32881 ttattttata ttgagatttg aatatccttt aacaattgat gagttagctg tttccaacaa
    32941 aaatagcgac tcgatcccgt tgtattttga acagacaaaa aaacaaacta tccgatttgg
    33001 gacatctttt atcagctcgg aacaagcagc ttataacctg gaactagaac aaaaccaaag
    33061 caaagagact taccttgctc acagtcaaga acaatggcag tcctattttg atcgtataca
    33121 gctcacacac catgatcaac aacaattgcg gacgttttat cataatttat atcgggtgtt
    33181 tttatttcca caaacatttt atgaaattga tcctagtggg caaaaaatcc actatgatac
    33241 gactagtcga tctataaaac ctggtgttct ctatacaaat aatgggtttt gggatacgta
    33301 taaaacggtt tatcctcttt attctttgat tgctgtagaa aaatacgaag aaatgctgga
    33361 aggttttttg aacagctata gagaaagtgg ctatttgcca aaatggctgt ctcctgatga
    33421 acgtggactt atgcctggga cattgatcga tgcggtcatt gcagatgctg ctgtaaaaga
    33481 tatccgacga gacttgatgc ctgaatttct aacggcaatg gaaaaaggtg ctacggttca
    33541 aagcaaacat ccaaattatg gacgccaagg aacagaagat tacttgacat atggctatgt
    33601 tcctaatcac taccacgaat cagtcaatca cacattagac tatgcttata gcgatttttg
    33661 tattagccaa gttgccaagg tactagaaga agaggaaatg acgaagaaat atcagcagca
    33721 atcattgaac tatcaaaata tctttgacta tgagtcagga tttatgcgtt caaaagataa
    33781 agatggcaag ttcagagaac cattttcagc taatcgttgg ggacaagatt atgcagaagg
    33841 aagtgcttgg caaagcagct ttgctgttta tcacgatttt tcaggactga tcggaaaata
    33901 tggtggcagt gagagatata aagaaaaact cattgagctg tgcaatcagc ccccaacgtt
    33961 tgacccagga ggatatggtt ttgagatcca cgaaatgagt gagatggccg cgatagaatt
    34021 tggacaatta gctatttcaa accagccaag ttttcactat ccttacttgt tcagttatat
    34081 aggacaacct gaaatggcac agccactgct taaacagttg atgactcaga ccttcgacga
    34141 ttctccaatg ggttaccctg gagatgagga caatggaagt atggccgctt ggtatatctt
    34201 caatagtctg ggattttatc ctgtcacgcc tggaaccggt gaatatgtta tcggtatgcc
    34261 attaattgac aaggctgttt tgcttttgtc aaatggaaag gaattgacga ttgaagcaac
    34321 gcctaatcgg ccgcagcaac aatttatcca taaaatcaaa cggatggaca aagaatatac
    34381 gaaattatat tttacccatc aagatttgct gaaaggcggc acgatctctt accaattagg
    34441 gatcatccct caccctaaga aatatcaagc aaaagatttg cctttttcct tatcaaacta
    34501 taattaacaa gaggttgtga aaaagctgtc ctgcatcaag taataagaac agccaagcaa
    34561 aaatagatcc tcatattttt cgtttggttg gtcttattac cgcagatgca agcttttgaa
    34621 caccgtttat tgagaggttg tgaaatcagc gttctgacct gagtattaga gagacaaatg
    34681 gcaaaatagt tcctcatatt ttgccatttt ttgagctaat accgagggtc agctgattga
    34741 acaccgttta gtggagaggt cgtgaaaaag ctgtttagtt tcgagtatta agaaaaattt
    34801 tgtgaaaatg gctttccaca tttttgcata aatttggctt aataccgaga aactggcttt
    34861 tgaactccgt ttatcgagag gtcatgaaaa agctgccttg cctcaagtaa taagaacagc
    34921 ccaaaattag atttctagac aattttttat aagttatttc attacattgg ctttttcttt
    34981 tatgattctc cactgttttc tcggtactct ttaggggatc tgccgacgat tttcttgaat
    35041 aatttagaga aatacgtggt gttgttgtag ccaatctcac ttgctatctc attgatgttt
    35101 tgattactgt gcaaaagcag ttgtttcgct ttttccatac gtaaatggtt caaataagct
    35161 gaaaaacttt ttttcgtttc ttttttgaac agctgtccta gatacatgac atttaaatgc
    35221 aggttttctg acacttcttt gagtgtaagt ggttcttgat aatgctggtg caggattacg
    35281 attacttttt ccacattgtc actgtacttt ttctgtctct gatgcttttt gatgaatgac
    35341 agaagtagct gctgaagttc ttgcaccgtt tttgcctgat ggattttttc gattccttgt
    35401 aaatattcct catcttccag atcaatcaat tcccgttgaa tctccataaa cagtaaaaaa
    35461 ctggaatatc gtatatcttc cggcatcatg gctgctaact gaaactgttc aaaaaaatca
    35521 gtgatcattt tttgagcgac ttccaattgt ttattttgca gcacgcgtct gaaacttgaa
    35581 aaatcgatgg tttgatcttt tgtttctgct tgtactgcgt aaaaaacatg ctcgtcttta
    35641 tctccataaa attgatgcaa ctgtagattg tctttcgctt gttggaatgt ttcagggatt
    35701 ttgtccaaag aatctgtctc ttccccgata ctgatcagcc attcctgatc aggaaagttt
    35761 tcttgaacaa aacaacagaa gccatcaact tcatctgcct ccaacaacaa cgtgatcaat
    35821 acacggtctc cgtaatttcg ttgatagtaa aagatttgtt gccgttcttg caaccatcta
    35881 ttgataagag tatcatgagt tcttgccagt tgaatcaata ctatcctatg ctgcttctca
    35941 cttatttgtc ctattatttc ttcttcactt atttcatcca attcttcatt caaccattga
    36001 ctgaataaca gttcttgata gatttcttgt tgattttttt gttcattttg ctgatctaac
    36061 cgtgttttga ttttcttgag gctttcaacc aattcgaact tattcacggg tttcatcaaa
    36121 taattcacgg cgcctaattg catacctcct ttcaaataat cgaattcttg gtatcctgat
    36181 aaaatcataa attcaaaatt atgacgttct ttttgagccg cttcaataaa ttccagtcca
    36241 tttatttctg gcattgtcac atccgttact actaaatcaa tctgtcgctg ttcaagtaca
    36301 gctagtcctt ctttggcatt ctctgctgtt gctactattt gaaagcctaa actttgccaa
    36361 tcaattatct tttttaaccc attcaaaatc atgtattcat catctaccaa taacacttga
    36421 tacacgctca tccctccttg aatgtaatag aaacggtcag tccacctgtc tcattattct
    36481 caagcgacat ataataactg ctgccgaagc tggcacgtaa acgttcattc acgttttgca
    36541 gaccgataga accgtgaagt tccactttag gatgagacag tttttcttca atctgtttta
    36601 gccgtttttc agtcatccct tttccattat ctttgatgat gatccttacc cgtttgcctt
    36661 cctgcaagac tttgacactt agcgcattat cgaatcgagt aaaatcaata ccgtgtttaa
    36721 aataattttc aactaatggc tggatcacaa atttaggtac ttcgatcttt tccaaatcag
    36781 gatcgatcat aaaatgatag gctactcgat taggataacg catttgatac aaatagacat
    36841 atttttcgca aaagtccagt tcttctttta aagtgatcgt tttctcttga ttcgtattgt
    36901 ttcgaagcaa agcggaaaac gcatacacaa catctgctaa ttcttcactt ccttcgctga
    36961 ttgcatacat tcggatatat tctaatgtat tatataaaaa gtgtggattg atctgtgctt
    37021 gaagcgcccg catatgtgcg tcttgctgtt tgatttctaa tttgtagatg tcttctacaa
    37081 actgattgat gctatccaac atttcgttga taccgattga aagttccttc agttcatatt
    37141 ccgtgtttgt tatgtcgatc cgcgtctcta aattccctgc tgctgtttga tccatggcct
    37201 gcatgacgat agatacttgc tgtgaatatc gtttgaatgt tttataaaga aagataagca
    37261 gtatattgtc taaaataatg ataccaatga tcagcggggc aagatttttt cccgtttcga
    37321 tttttacact ttttttatct agtacagcta atatacgata atcatcagac agttcttggt
    37381 gttgaacaaa attgtttttt tccagatcgt caatcggcaa ttgtgaattt ttcttcagac
    37441 tctctgtgat gatcatttct tgtctttcaa acgtttcttt agctatttta ttatcatgga
    37501 atgtgtatag tcgatttgtc gttccagaaa tcatgtataa agagagtcca ttgaatgacg
    37561 ttagattttc taaaatcata tctagttctt tcttttgaaa accaataaaa atacttcctg
    37621 actcagctcc tccttgtgtg ataggcaact tgatataaaa ggcgtctagt aattttggtg
    37681 tccccttttt tatttcccca cctttgtttt ctctagaaga ttcataatac tccggtagtt
    37741 gattcaacac gatatatatc gcacttagtt cttcataatt tgcgtagaga tctttgattt
    37801 gatttgggaa agaaactaat tcatcggtac tgttttgctg ttcgtataca tacatcagat
    37861 actgatctat gggttttgtt aagtaggtcg tcacattttc gatcttttcg gaattcccta
    37921 caagctggtt tttgatgatc ctactcaagg ttgttttatc attcagcatt ctgcttgtgc
    37981 tttgaacggc atcacttgtc atgttttctg cctgtttttg attttgttcc caagtatgga
    38041 tactaaaaat aacggtagca gtcatcgtta caccgatcat gataaccgcg tatttccgca
    38101 acagacgatt tagtaaggtt ccttttgaaa atagaatccg aaacttattc ataactttcc
    38161 aactgtttat ctaattcttc acgccaatgt gttgtcatcg taccattcca aaccgtcaaa
    38221 agaccaatga caccaaacag gatcaatcct ttgaattgcc aagtcacgcc taaaattata
    38281 ccgctaccaa taattatttt tagaaaagac gaaaagctgg aaaaaaccga aataaaactt
    38341 agctttagca gattaggaac agagatcgta ta
//
LOCUS       DANKKG010000011        37152 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000011 DANKKG010000000
VERSION     DANKKG010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37152
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.011"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(130..480)
                     /locus_tag="PW004_000485"
     CDS             complement(130..480)
                     /locus_tag="PW004_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725481.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDAENEQRANQNLAKEQMTTE
                     EITEVLNAAVIKNRSVCIQKEERNAEGYYPDDIIGKIFGSDELGIYVGKNKVHYDEIR
                     HIEFYQKHKWSCLE"
     gene            complement(473..1798)
                     /locus_tag="PW004_000486"
     CDS             complement(473..1798)
                     /locus_tag="PW004_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HDL2725482.1"
                     /translation="MFDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMKLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQERIKEELGLIA
                     AVGVGDNPLLAKLALDNEAKNNEGFIAEWTYQNVPDKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIKDLANWNPYTIKSRLGVIGLQLYFHANGIDRTDIAIPPEPSKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHHCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTRELQDYCLFLFNKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDDEK
                     LDKIIDKIRQKYGFTSIIHASSKLEGARSITRSTLVGGHAGGVGGLKND"
     gene            complement(2070..3317)
                     /locus_tag="PW004_000487"
     CDS             complement(2070..3317)
                     /locus_tag="PW004_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HDL2725483.1"
                     /translation="MKNKLTLLICATILSTWAVPTQNAFAEETGNDTTQQTVTSNSSS
                     EEQTAIVNGEEVKLEAPVKEGQSEAEATQAPKEELQQDQMSRFARSAVRTIPMYRMYN
                     PNSGEHFYTQDTGERDNLRRIGWNYEGIGWQAPTSGDPVYRLYNRYNGEHFYTLNAKE
                     RDNITKQGWTYEGIGWYSYKGANGIPVSRLYNKRVNWHHYTLDEHEKNVISKQGWNYE
                     GVGWYAVGNGQPSNDDYKLLGVKNYNQYALGAPSGCEGASLLQGLQYKGKITNWSLTQ
                     FLNTIPKSPNGNPNNGFVGSPFVENSWTYSAIYPAPLATWGQKYGNVQNISGSSMNTL
                     LNEVKNGNPVVAWVTINFQPIRWGNWSFGVAANNNHAVTLDGYNKGSNQVHVSDPISG
                     SYWLNRTTFENIYNARKYAVVVR"
     gene            complement(3585..4208)
                     /locus_tag="PW004_000488"
     CDS             complement(3585..4208)
                     /locus_tag="PW004_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2725484.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
     gene            complement(4639..4851)
                     /locus_tag="PW004_000489"
     CDS             complement(4639..4851)
                     /locus_tag="PW004_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725485.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            complement(4968..7262)
                     /locus_tag="PW004_000490"
     CDS             complement(4968..7262)
                     /locus_tag="PW004_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HDL2725486.1"
                     /translation="MAVTKIHQIKTTLNKAIGYITNPEKTNNGMLVSSYNCQSQFAEV
                     EMQMTREYAREVKGDYRKVGGSEVLAHHLIQSFSPEDKVTPELAHELGRQLIDELTEG
                     KFEYVIATHIDQEHIHNHIIFNSVSFYDLKKFRSQPYRTANKIKEISNRICAEHDLSL
                     SPKKEKLKDSYKSYSNRRNNTSFRSEIRKRLIMCLNESTSWEEFEEKADHLGVNIDRK
                     GKHISYALLSEGQQRRTRGDKLDDLETFTESNISQKLSANQSYINQLKEAIEETFFSS
                     DNLEDFSFLLEQEHGITFQLNKQDQFVFHFNDVNDFKWNESLLPDSYQIKNLKDYFRE
                     SYEFTNDSSLNSIEERYREKEKSVIVQEDTPIQLQEIQIEKATKEGLLVSIEHDDLNG
                     LVFVPNNYVDVNEETGEYTIWINDKFDYTLTDKQTNEQTSFTVKGEGIIRGLEKMQGI
                     QPQEIKLSSKNILSMSNRGVSISIPELSIKRLFIPSEYVVIDEISKSCSILIGEKWNY
                     YAEPLPENDDNSNKKLPYQKFTGRSLISSLKETPELLDVFLKPKFDQLHYQSRKRYTN
                     QLVEALNTIREEKIVSASQLEQRLNEIVEQKNQAKEKIAEIDGKIKNYNNVAKLLVTY
                     EKYLPIMNEIERATMLQKIKLEQKYKEEVRQFKFAEKKLLASDNLRPDLTKEKIISVA
                     KNQEKQRKRLIEGYKHYENKLNRLIDVQEILQELDRNGLFDLSGYRLIDREEKLTDKI
                     EKYEKEVEETKQKNAKEKRKEHEI"
     gene            complement(7262..7675)
                     /gene="mobC"
                     /locus_tag="PW004_000491"
     CDS             complement(7262..7675)
                     /gene="mobC"
                     /locus_tag="PW004_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HDL2725487.1"
                     /translation="MEQKLAKTPENILKIANTYDDSERTFRKETRFSEREIFLLNYKM
                     SQANTRNFSQYARIMCLSGLMITTDFSDLKNLTTELGRIGNNINQLAHRANISNSVSK
                     EDFLELKTNYDQLTKLINEELLFRMRRARDNQRFI"
     gene            complement(7910..8179)
                     /locus_tag="PW004_000492"
     CDS             complement(7910..8179)
                     /locus_tag="PW004_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725488.1"
                     /translation="MQTFTQHELEIISGHKQITGSNANKLNVIKSEVGEMLSMLKSDL
                     GKQYIEDDEQRHYTLKNYQSVYDKLEKMSEDEFKQIDLSGIEELE"
     gene            complement(8200..8535)
                     /locus_tag="PW004_000493"
     CDS             complement(8200..8535)
                     /locus_tag="PW004_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725489.1"
                     /translation="MKQYLDQWNIIEGALTEERINEIPDCLEKDHLFLIRRMLQKENF
                     EPNNFIVVEYPATGIYCCNHINNDQYFIVQEEKNKLVPYYTTCDMNEKGINNFPCTSI
                     KESMKLIEM"
     gene            complement(8617..9648)
                     /locus_tag="PW004_000494"
     CDS             complement(8617..9648)
                     /locus_tag="PW004_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725490.1"
                     /translation="MSYKDSEAKKTNKTAELLSQAEEGIKEVLSSDKYKNFLRVMSKF
                     HNYSLNNTLLILMQNPNATHVAGYKTWKNSFNRQVNKGEKSIKIIGGRPYKRKVEEIK
                     NGQKEEKEVEGISFFPVSVFDVSQTSGEPLPQLMNELSGEVKNYPELYVALKQSTNFS
                     IEFADIQGGTKGYCSPLEKKIVLNNGMSQAQTIKTLIHEITHADLHAPEFDKNSSIKT
                     TKSTKEVEAESTAFVVCEHYGIDTKDYSFPYLAAWSSDKELKELKNSFEVILKQADNL
                     IQKIDKNLAELQKEVTKEKEEKQSTLSLSDQLEEFEDKAKSLNEQRDKEKLINKIRQP
                     KEQTDVSTL"
     gene            complement(9667..11247)
                     /locus_tag="PW004_000495"
     CDS             complement(9667..11247)
                     /locus_tag="PW004_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725491.1"
                     /translation="MHNKKKFLVVGLFSAVLLGGAVTYGMYSQQPHNETEKTEQTTKS
                     ESSKHKSKKSAWEESVKGKEDKKEKNKHSTKKADDILAVIADTPLIKQQPNQNKNNLI
                     DRLAVALDDQTKKEADRQEKLIIKDKKSVDEPVLVDLSYKPQLEDTKPSNKENGKTEN
                     NKDENSELDKGNDTKPDNSLPDPLPVPEPNPLPDPTPSPDPNPIPTPDPDPDPEPDPT
                     PDPEPSIDEQINQSKEELNDSRKQADELNKQLTQVKDSLDNLQSIGASTEKNVDKAAL
                     QWQKVADLVNEYNQLTNEIKKLIEEDGTVLTINYDLYKETYEKLNQKVTEVKTAQKEA
                     NNTTNEINKNVEQATDTLNNLEETKKEYQNKQKDVSEVDSNVNSAINHAQSNEQVASA
                     VQDEINQAAQSNEQLNETNKTVGEQLNQVDPIESQTDIASAKEQTVDINNQAEEQNNN
                     VADVMNDFNNLPKPSDSEEPSIPENTDQNQDSTSQPQNIDNASVSQNKIENEQVSEKT
                     LPVTNQNSNQSEIEDIQK"
     gene            complement(11240..12307)
                     /locus_tag="PW004_000496"
     CDS             complement(11240..12307)
                     /locus_tag="PW004_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725492.1"
                     /translation="MFSKKKYVQFIEQRDIELPDTPVEIYRGIYLLEQENDRLTDKHT
                     KGSTLILLTDKKGNELNRRLIQLPIEQGIETILTNLLIEPTSNKKQSSSKSQMILNDI
                     EEPKIKNKKKTVNYQTVFFLFTTNLAKKIYMALIIVLFLFFTSFFVLNNHSSTSTDSQ
                     SYHELLKKKDYEKIIKQYPEKENELVEKLYSEKNSKELKELAEKSDSQIALFYWDFLN
                     EHWQAVTTMKNVTPNETIQAMRGYAYLKQGKLDEAELINKEIDNQTLKDQIYQFKKQE
                     AYKALHEKDIEKAEKINKEINDSELSEDIKVAKSMVNLLQKYEKDRSDSKLSEDERKE
                     AENNYKLWSENLKQLGGNDNA"
     gene            complement(12328..12714)
                     /locus_tag="PW004_000497"
     CDS             complement(12328..12714)
                     /locus_tag="PW004_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725493.1"
                     /translation="MLIAKRILASIVAIPCFLLSIIVIAVNIISIPILLVIQILKKVV
                     ILFITLVWIGFIGLFIYGLSSLGFSETISMFTVPTIGILILTLILSIFTFISDKIPNF
                     LGIVSSFFLSIPKFLWGAEETEEAEE"
     gene            complement(12787..13020)
                     /locus_tag="PW004_000498"
     CDS             complement(12787..13020)
                     /locus_tag="PW004_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725494.1"
                     /translation="MARKSLTERIQQKKEEATKLEKRIQEDQYKLEKLTKQIRDLEAE
                     QVLAILDNHKLSVKDLNKILSKQPDNNNFINHN"
     gene            complement(13020..13394)
                     /locus_tag="PW004_000499"
     CDS             complement(13020..13394)
                     /locus_tag="PW004_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDL2725495.1"
                     /translation="MNKKIFSICVLIFTVSIIFLADHSYIKYIEAQNDPVYLDESNQE
                     KIVFYRNDCRDCEKIMPYLIRHNFLYHDLTFVNMNNKENKRYIKEYGLTSVPTIISLK
                     QRYVGTDISKIKTLVMSKGDDF"
     gene            complement(13387..14016)
                     /locus_tag="PW004_000500"
     CDS             complement(13387..14016)
                     /locus_tag="PW004_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725496.1"
                     /translation="MLNRKSATITIFVLIVSVFSLSIGLNIKQELTVKTANSKIAALE
                     KKNDILKRETKDQKSRISKLEDENNKMIETQIVNKDGDVYKEFTNIATKYFKSRFNYD
                     PKTYKESKENVRDLISEELYKKFSENQLTYGDSNNVSSRLDNLEIYSGSLINQKTITG
                     LLIVDYESKLGETDWFKNSEIYTVQYDIHAKKLTKIQSLGSVLRGDMIE"
     gene            complement(14036..15883)
                     /locus_tag="PW004_000501"
     CDS             complement(14036..15883)
                     /locus_tag="PW004_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDL2725497.1"
                     /translation="MNTDFRERIIRKQKTAKKFVVNRDNNQFKKKIVSTDYTKRAKKA
                     NHSGKIKSSRGTSYLIKKDSAKNVVKDVNSKAQKNGVEQGNATAELETSKTIGRGIKN
                     STKATKTVAKTTTKVTKKSKELIQTKASKKEVLTVVKKGSKKGLVNTSKAAKDTVINE
                     IQEFRGDSESQGINEAIQLKDFIDTGISGAKKTKKAANTAKKSSKALTKFGKSSYKYT
                     QRIAEIGIRRVQQVLSKEVAKALIAKLAIPLTLAVLILSIVTAVISTVNIFNFGAGDC
                     DTSANYDNITTKDIESNAKSIYSIIRKEIPESTPQGISGALGAMQFESQLNPSAVNPS
                     SGATGIAQWLGSRLTSLKEFAHKKGKKETDLGVQVEFLLSELNSSYYQSSKKILAMTD
                     VHEACKEWVMKFEGLSQDSSQWYLDQRNSYADHWYATLGTSDPIAEGTISNGADSELN
                     TLTCDLESDGGDIMEIAKSWLGWFHYDQIHPAPDLGTDLKNPNKNGRTDCSGFVWLVL
                     NKAGYKVPANMQWFTGSMASDARGSHQYLKQISETEAKAGDIVIVNQGAGAGSNGHTG
                     ILTEKWRGKDTKIIQEGGNGDSVNIEAFGTSFMSLLDGGDVVLARPIKK"
     gene            complement(15880..18330)
                     /locus_tag="PW004_000502"
     CDS             complement(15880..18330)
                     /locus_tag="PW004_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL2725498.1"
                     /translation="MILEKNDSKKSKKQPKDKQAKKVLKILAKQKKQRGKNQRRDAPP
                     LSSQRSLTYKAMYENGVCELKEGLYSRTFKFSDINYAIADQSEQELTFSSYCELLNSC
                     DSDTSLQISIINRSKDKKQFEKDLSYELVDDGLDVYRKEMNHMISNKIKYGNSSLQKE
                     KYITFNQRAKTLEQAIPALNRLQGDLEDQFSEMGCEVEELSGIERLRVVNDILRPNKK
                     LHFEYEDLLFSGLTTHSAIAPDSFDFKYRKDRFKMGDYYGQVLYLRNYPAELSDKLIT
                     NITDIPTNLQITLHIEPLDQQKGLDLVKTKLSFMEQQKIDEQKKAARSNYDPNLIPME
                     LRRSMDEAQELLDQMQGDNEKLFSFTFLVMAVDKDERKLDDTIEQILTAGRKNDCEFA
                     KLNYLQEEGLNSTLLWGNNFVPIHRTLTTASTAVFIPFTAQELMQPNGKYYGLNSVTK
                     NLVTLDRRKLKAPNGFILGTPGSGKSFATKKEMTAVLLSDPDAEVIVIDPEREYTPLA
                     EALNGEVIQISAGSKNHVNPMEINNDYADDDDPVILKTDFVLSLCEMLVGGNIGLSPT
                     QKTLIDRSCRFAYKKYFENGSGMPTLKDFSNILKDQPEDEARQLAVELELYTVGSLSV
                     FSHQTNVDTSKRFIVFDVKDLGKQLRSMGMLIILDQIWNRITSNRNAGRRTWVYIDEI
                     QLLFSNEYAENYFFELWSRARKWGAIPTGITQNVSTLLSSLNAERMLSNSDFVLMLNQ
                     SQRDRERLGELLHISSRQKKYITNAKEGAGLMYAGDSMIPLYDEFPKDTNLYKVMSTK
                     IEDVYKYQNEQREQGTTV"
     gene            complement(18278..18649)
                     /locus_tag="PW004_000503"
     CDS             complement(18278..18649)
                     /locus_tag="PW004_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HDL2725499.1"
                     /translation="MAIRVKVPKEIKDYQEKLMGGMSVRQLVFFVLAVLISVGVTAYC
                     VLVLHMSVDAAGYIVIPLLIPIVAFGWIRKEGMPLEKYGKIILNYYSKTGIRIYQTKE
                     RSNLNDFRKKRFQKVKETTKR"
     gene            complement(18914..19762)
                     /locus_tag="PW004_000504"
     CDS             complement(18914..19762)
                     /locus_tag="PW004_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725500.1"
                     /translation="MDGFLTWCVESILEVISFSSKTSHLTSNLENFSPQLYKYSTTIL
                     DSVSKPIAFSILALFFALELQRISNRVETNGGGQTGFEMIFKSLVRLGLCKLAMDQVS
                     LIMRAIMDITGYMTDKINHLGMGNKEVSDMLDAKKILAPILKEGLMFKVGVSTVLIIA
                     LLITIGSIVIVQVVVNMRFIELYAFLAMSPIPIATFPNDEWSSIGKNFLKSFTATALQ
                     GTLIFMVMQFYPYLLSSAFSDITNSGSASETFLMTLIGIIGNSVLLIFAVMATGRWSK
                     SITNAM"
     gene            complement(19794..19994)
                     /locus_tag="PW004_000505"
     CDS             complement(19794..19994)
                     /locus_tag="PW004_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725501.1"
                     /translation="MGTFNAIIDILSKYAIVGGGLYAVWGAVVLGGALKDHNGPQMQS
                     GIWQIVGGALIVAAAALFKTIV"
     gene            complement(20011..21885)
                     /locus_tag="PW004_000506"
     CDS             complement(20011..21885)
                     /locus_tag="PW004_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HDL2725502.1"
                     /translation="MNITKKDLVFGIVLAAFFFYLGTLFFMVYFSIPQNTDSSWINVV
                     NAAFDKFLEPKTFTISPFTFSKIAMKGGAIIAVIVLLIDLYIISGKKNLRMGEEHGSA
                     KWGTSKDIAPYVDKKFKNNLLFTNTERITIETKLKKNKEYERNNNVVVVGGAGSGKTR
                     FYVLPNLLQMHSSYVINDSKGGILSDTAAIMQQNGYRIKVFNLNTMKNSDGYNPFHYI
                     RRTEGDCEIDILKVVNNLMKNTSDPNKKGGDSFWDDAQRSFLAAIFHLLILKGKPEEQ
                     NLGIVADLVRRARVKEDDEEYISSIDLVFQELEQEQPSCFAAKQYQNFKMAAGKTAKS
                     ILISLGVRLSPFDVPDIRKLTSTDTLELDTIGDRKTVLYIILPDTHTTMNFLASMIYQ
                     QLFDTLVYKADNVYNGSLPIPVRCLLDEFANTGQIPDFENVIATTRSRGISVDVILQN
                     LTQISKKLYKDSWETIIGNCDSFLYLGGNEQSTHKYISTQLGKETIDVVTYNESRGTT
                     GSFTKNSQKQGRNLLDPNEVREIKGGKCIYMLRGTKPFLSDRFKLERHPLFKKLKETP
                     SGYMYQSPLDQEEFIANNDPIDFELSKNVDMKEFLKHTEIETYDFEDEEIETINLDEL
                     "
     gene            complement(21882..23048)
                     /locus_tag="PW004_000507"
     CDS             complement(21882..23048)
                     /locus_tag="PW004_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HDL2725503.1"
                     /translation="MNITVKELGEPIELEDYLEEYKFQVIFLLKNMTFIDRKDNRVRQ
                     LFNMELIRAETKWYSFYQKNHRPPDYNFLQEEITSFGVNRLPMFDRRNEGRVNDELSV
                     DNFVHYHIQQLKLEKILSPIFFEKNDLRFMERYAKNKAKAYFNERDQYIKGYESFRIS
                     RNKIMQRAGYEQLKYEFVSDNMVQGYFKEEVVKLAVNDERVAEFVLDEGKNLMRGLLQ
                     GLSKAGIAVVTVPAKAVHSQIKKQRTTGKQRYNQLKKHGSLEHIDVSKTDLKPLANEL
                     KKYKVDFAVKRNKETGKIHVFFKAQDRDTLNLALENVLQKFANEKDQNREQQQDKSNK
                     DIEKSSADGKDKDKSLAEKLDKLEDKAQEINQQREQQREQLKEKSKNKTKGQER"
     gene            complement(23072..23479)
                     /locus_tag="PW004_000508"
     CDS             complement(23072..23479)
                     /locus_tag="PW004_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725504.1"
                     /translation="MTNRNEGITIYLTIGKEVKLNFDSENNLSSIRLSINKVAELFGN
                     KVVNEESIFYVSTVALVTKSNYKTFISVVGGKQVISGDAVYEEVMIDQERTSNKRGNK
                     NVYEQGYFEFFVSEEDKIPKEKLILPYNVKKRR"
     gene            complement(23658..25184)
                     /locus_tag="PW004_000509"
     CDS             complement(23658..25184)
                     /locus_tag="PW004_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HDL2725505.1"
                     /translation="MKKKHISKVASAVLLSQMLLTTPLQVLADEQLSTPTSQSSTMNN
                     QSEESTSSSDEQTGTSTTQTATDSTESSTDQSTTDDTTTSSTDTTSTTESSEKPTEDS
                     TTDSTSTTESSSESKPDETKPSETKPSESKPEKPNTGSSTNESQTQPTTEPSNNQQAP
                     SVAPNNQAKATNEKQQAKPAENESKPYHQTPTAPIQELVPSSKETAEGNIHFEKDESV
                     ESFVRKIGESARKVGQENDLYASVMIAQAILESASGQSQLAQAPNYNLFGIKGTHNGK
                     GVSFATQEDLGDGTFYTTQATFRQYENYEDSLNDYAQLLKEGLTGNSQFYSGVWKTNA
                     KTYQEATKFLTGRYATDTKYDQKLNGLIETYHLTDYDKEVAGPQLSKEGYIVPVKNYT
                     ISSPFGTRGGEFHRGLDLAAAQGEPIYASKAGTVVKAEFHPSWGNYVAIEHEDGTTAL
                     YAHQQEYQVKVGDKVKQGQIIGYVGSTGNSTGSHLHFELCLDHSLSQSQLVDPETVLF
                     "
     gene            complement(25271..26308)
                     /locus_tag="PW004_000510"
     CDS             complement(25271..26308)
                     /locus_tag="PW004_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HDL2725506.1"
                     /translation="MFLGKGEINIVKKIIKTLPLLWLLSSLILAICGFYGYTVLLRPE
                     NTIKQAQQITHPIQSEKEIKEIQQTTIGKATYNENQIQPVTPTEYAEAQLSYEDLVNQ
                     WGIGSLYIPSSGIYSKILAGMSNDNLMVGLGTYYPNQLLGKGNYVLMAHNLVQGGGVL
                     HNLPQSSVGSTIYATDFSKIYEYEITTNKIVNQSEGKLLDIPQEGDSPLMTIFRCEGG
                     LHTANRALIQARYVRSYSAENGSHDIKQALGLETTRNKTVNKQRLIDQQATSTKKTEA
                     AKESITPDKDTKKAKQTNKIEWCFTEKKAIYSNFQVFSILIFQLANAYPILIGLVFLV
                     GLSSCILFNRV"
     gene            complement(26357..29521)
                     /locus_tag="PW004_000511"
     CDS             complement(26357..29521)
                     /locus_tag="PW004_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HDL2725507.1"
                     /translation="MKNKRNKWKVPKYLLMILGALAMFLGFGLSSQSVNADNITMKPG
                     EKGDIYYDASNVYTMHGTKHGQPWSDATAAMAVSPNNGATDRIVFCIQPGVPFITTNN
                     PDYEAVAVTDIPKDAQIASVIWNTFYAFGTNFTNEDRIVTQAVIWELLPQYGIKVDSI
                     SGIPDFQAKKQKLYDGIKEYKRLPEFNNKTIDLTFGETKKLTSSVDLNSFETQVSNSA
                     KVNWNIAADGKSVDVTPTDPNVTGGSISFKRSYMEGTPIALEKTGSQTVYLPSVKDPN
                     NYSVNFRIQTVGDITIKKVDKETGEVVPNTQFKVEFSGKGAPSAQTVTTDKNGLATIK
                     GINHGVHVKATEIAVPAPYVLGSAIGDSDVVEGDVVAGQTVTLTQRNQKAKGQILIEK
                     SGVETGKDMWNANYTLAGNVFEIHEGTETGAVVRTITTDEKGHAETGKDLPLGTYVIT
                     EKTASNGFANTFKPVTVKIEYANQTTAVIVKGAEGTNQEVVGSTLLTKEDAETGKETQ
                     GRATFDGAQYGLFHKDGTPVKWSENFKPTTSKGNKLEGDEVKFELSDEEQQASVEHLA
                     LGDYYWQELVAPEGYQIDNTKREFSITYKDQETKVIETQSTSQENVIKFSLDGFKYVD
                     SKSGDTKTGYNGIDFTLTPIDPTKGEQRKVTTETDENGYDGYWAFNEVPYGDYKMSEV
                     KAPEGYKTIKDLIINSKFDAEKREYTFTITEDGQKEPIKTLTVPEAKINEGSNVIHLS
                     KLFLTNKVKEMPTIRTKATVDGEKTFTPSKETPMQDVATITNVEKGVEYTNKIKLWRI
                     VNDDYDNAKVVWEGSHDFVAEGDTAEDVIKQMVDTSKDDNTVSYVFTEELFIKDGAKV
                     AEHNDLTNKEQTIRPVVPELPTIETLFVTKDGGKTFDPTKDQTLIDEVKATVPKEDIG
                     QTFYYVTQFHKIDKNGKVTVVGTDESEHKAGEMTYEFKAVYNYKANTLKDGEKLVATH
                     IAYTDKEHKNEYAKHFDLKNEKQTLTAKTPVTPATPTTPQKSIPQTSGSLINIGSLIV
                     LGIAAVAGAALYVTTKKKVNKNEK"
     gene            complement(29726..30619)
                     /locus_tag="PW004_000512"
     CDS             complement(29726..30619)
                     /locus_tag="PW004_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL2725508.1"
                     /translation="MKLLKVLAVGVTVGGIALAINQEKVSAEEWTPRTVEQIKADIKG
                     NEYTIVWGDTLSGISQATNITVQKLADINKIANVDLIYAGNKLVFEGNVATVQNSAGE
                     TVAQTVIQDTDKVNPTQPVGQPAVSQDTTSNTTNSADQSVAPTTPAGDNGANTPEQPV
                     QPSTPSDNGNQGGTVTPNPDQNQGGNNNQDNQGNQGGDTGNNGNQDNQGNNNGNNNNG
                     NNNGGNDGGDVTPPQPVEKWTVWYSASDEANATYNIEKGTKVFDTEAEATAWIDQYAD
                     GLLAQGITSNSYGVSSFLVQE"
     gene            30770..31012
                     /locus_tag="PW004_000513"
     CDS             30770..31012
                     /locus_tag="PW004_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725509.1"
                     /translation="MNITNIEELKEWIETNLVTKQEAIKITEQSPKGFQQSVRTNVIK
                     PFYESKEGKGPSVVRLYLKSDIETYAKQLKEKKQKP"
     gene            31058..31477
                     /locus_tag="PW004_000514"
     CDS             31058..31477
                     /locus_tag="PW004_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725510.1"
                     /translation="MKKSHIVTLATIFLLCVAAFFFFTNSPKNNKHITARTATTESTI
                     KTETVYPSLNELKQSQGSDIEILGKTQITTVLFQNKNMAILTTENNKEIQLPLIVTDK
                     KNNLFEIPSLTYSKKNLKVGEVFGLAKKDNHYLAYKL"
     gene            complement(31588..31860)
                     /locus_tag="PW004_000515"
     CDS             complement(31588..31860)
                     /locus_tag="PW004_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725511.1"
                     /translation="MIEEKKKVLYSNKPEFKKLVMQYAKKNIGRSITYDTFIKWLDKY
                     GYDLSQYDTCWQAVFKSLLQRNFQIDIAYRKTKECQLITVFQLNKS"
     gene            complement(31871..32068)
                     /locus_tag="PW004_000516"
     CDS             complement(31871..32068)
                     /locus_tag="PW004_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725512.1"
                     /translation="MEWLKNEKELFQFLSDMKRKNQVANLDNYIKWIKDQSVQIKQDR
                     ESGNLNIKIAVTDKQNNILIY"
     gene            complement(32092..32478)
                     /locus_tag="PW004_000517"
     CDS             complement(32092..32478)
                     /locus_tag="PW004_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725513.1"
                     /translation="MNRIRFGKSNKNEFSSIFKVNVGIALVFFSLGIWGGMRVQCEQS
                     YFDNTIDEVYFEYDLATHRNYNPKESHEVSLRRVQYLTWDLYPLLKYPESVTSKLFYI
                     DKRNNEKVEKAWVTLLKLQKIALEEK"
     gene            complement(32532..32708)
                     /locus_tag="PW004_000518"
     CDS             complement(32532..32708)
                     /locus_tag="PW004_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725514.1"
                     /translation="MSTEKKRKKYAFSDETIEKLDWLVKEHQKRSKKRIYPCDTLSEL
                     INNEYVIRKSFGNS"
     gene            complement(33275..33667)
                     /locus_tag="PW004_000519"
     CDS             complement(33275..33667)
                     /locus_tag="PW004_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgR"
                     /protein_id="HDL2725515.1"
                     /translation="MELKPKNLKQITCEFVLFNRQHIKFSNRTIKRKITDFSGKEIIL
                     YAKKLSSEQELTCFFDQIKAIYFDGKIVACRGCSELPLIVSNHVFSKMKARQKLLRES
                     KVRYREMQKEEWEKRKSAARKKYKRISA"
     gene            complement(34543..35586)
                     /locus_tag="PW004_000520"
     CDS             complement(34543..35586)
                     /locus_tag="PW004_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HDL2725516.1"
                     /translation="MSEFKYYKSKEVYREKYYQMPKVFFTNEKYLNLSNDAKITYMLL
                     KARFDYSVQNNWVDSEDNIYFIYTIEELMRLLNCREGKISKIKKELEAAGLLKQKKGR
                     IKKANGKIETTPNLLYLGKPEVTSEDVFKINEEERLIPNPVNAKIANTEKDDDIKVIP
                     VNAKIANTEKASDTNSSPVNAKIADNILYSNNSLDTNRHNIDTDKDQLQDQLLLENFV
                     DIMTDSSIDTFIPTNVLKLIKTFSNSYSEAQQTVKTIHNAKHKAQESKNTIIVYEEIE
                     YYGVDADKGLYMTLLKAYQKQKTEKVDNLQNLIFIYVKNWFAEKAIPAKLAHEKNAET
                     NSLPAVNLENWLD"
     gene            36015..36821
                     /locus_tag="PW004_000521"
     CDS             36015..36821
                     /locus_tag="PW004_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2725517.1"
                     /translation="MTKVLTTGNFKGGVGKTTNAVLTAYTLSNKGYKVLVVDLDPQAN
                     ATELLFATMTNVYKIKPEFKETLFVSIQNNKISNSLISVKENLDLLPSFTDLEKYIDY
                     LAELYDDDYSKDTHFSNLLNEIKENYDFIIIDVPPQLNKFTNSALVASDYVIVILQTQ
                     ERSLKGAEKYIEHLIQLNNDYGTEIDILGLLPVLVQNGNDLDLDIIEDAESLFGKSNI
                     FNTKIKTMQRLKRYDRTGITDNPKDIHDKRVQQVYQSVSDEVIQRINLLG"
     gene            36835..>37152
                     /locus_tag="PW004_000522"
     CDS             36835..>37152
                     /locus_tag="PW004_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725518.1"
                     /translation="MTNEKNESIGLNAIKRKNKTQRPENIVPENSEALNNTTDSGKIL
                     EQSKKRLTTKDLPKSIRMPLDTHTAISTIATIEDKKIYETLNKIVEFYINNMNPANKK
                     IVKN"
BASE COUNT        10834 a         6801 c         5377 g        14140 t
ORIGIN      
        1 catctattaa tacgtaaaac ttaaaacagt cgattcctat ttcattaatt agttgttgac
       61 catatcccca gatttcatct gaaagatttt caagtgttat atacttaagc tcatattgct
      121 ttataatgtt cattctaaac agctccattt atgtttctgg taaaattcaa tatgacgtat
      181 ttcatcgtag tgaactttgt tttttccgac atagattcct agctcatcac ttccaaaaat
      241 tttaccgata atatcatctg gatagtatcc ttcagcattt cgttcttctt tttgaataca
      301 tactgatcta ttttttatga ctgcagcgtt caatacttct gtaatctctt cagtcgtcat
      361 ttgttctttt gctaagtttt gattggctcg ctgttcattc tcagcatcga tttcttcagt
      421 atgatctgat aaatagaatc ctgcccactt taaacgtcca cgatcattgt aatcaatcat
      481 ttttaagccc tcctacgccg cctgcatgac caccaactaa cgtgcttctt gtaatacttc
      541 tagcaccttc aagcttagaa gaagcgtgta taattgacgt aaaaccatat ttttgcctaa
      601 ttttatctat tattttatct agtttttcgt catcaatctg tttttgcgga tcactaaaca
      661 aatctaactg aagcgaatca gtgtatacta gcttgctata ggttattccg acgtgtcgga
      721 cttcttgccc ctcatagtat ttattaaata agaacagaca atagtcttgt agctctcttg
      781 tattatctgt gataggtatc ttcatctggt gtgaaaaccc tggtctcgtt tctaagatcg
      841 acgttccaat gtgtaaatga acacaacctg ttttacagtg gtgttgccta atacggattg
      901 ctacttgctc agccatttct tttacgacca gttcgatttc atttcttcta gtataatctc
      961 gtggtaacac ttgtgaattg ccataagatt tttcctttga cggttctggt gggattgcta
     1021 tgtcagttcg atcaatacca tttgcatgga agtacagctg taacccaatc acgcctaaac
     1081 ggctcttaat ggtgtatgga ttccaattag ctaaatcttt gatactcatg atccccattt
     1141 gattgagcct ttttttcatt cgagaaccga taccccaaaa gtctgtcatt tcaggaatat
     1201 tccatacttt atcaggaaca ttttgatacg tccattcggc aataaatcct tcattgttct
     1261 tcgcttcgtt gtctaaggca agtttggcta acaagggatt atctccgaca cctactgcag
     1321 caatcaatcc tagctcttct ttaatacgtt cttggatcat ttgagcgagc ttcttccttc
     1381 gctgacttcg tgttccttca gtcgtaaaaa gattcagtga tcgggtcact tttaagattg
     1441 attcatcgat cgagtaaatc agtagatctt catcagccac atatcttctg aaaatattat
     1501 ttacctgcat atttcgcttg atatatagtt tcatacgtgg tggaacaacg tgtagtgttt
     1561 taggaaatgg ttgtggtaaa tcacgtggtc tactcacatt tgtaatacca taccgctttt
     1621 ttgcttcagg agaagaagct aatatcaatc ctgaaccagt attgtcagct cgactcataa
     1681 caacaagttc tgttgttaat ggatctaaat ttctttctat acattcgaca ctcgcataaa
     1741 aagacttcat gtcgattaga aaataatcat ttactggttc tttcgaataa tcaaacatga
     1801 ataacacccc ttctttttac attatacaaa cgtatgttcg atattgtaaa gaagaaatag
     1861 ctaaacacac aaaaaaagaa gcgtaggtaa actcttaccc tgcttcttct tttgatttgt
     1921 atgatcaact cattgtatca ctttattaaa taatttgaaa atttaacaaa aactctcgct
     1981 ctaaatgagt gagagactgc aaattagagt acggaatttt tgggagttcc gttccttaat
     2041 attaacaaat gatcttgaag catacaatgt tatctgacta caactgcata ttttcgtgcg
     2101 ttgtaaatat tttcaaatgt tgttcgattc agccaataag aaccactaat tggatcagat
     2161 acatgtactt gattacttcc tttattgtat ccatcaagtg tcactgcgtg attattatta
     2221 gctgctacac caaaactcca atttccccaa cgaattggtt ggaaattgat agttacccat
     2281 gcaaccacag gatttccgtt ctttacttca ttaagcaatg tattcataga actacctgaa
     2341 atattctgca catttccata tttttgcccc caagttgcta atggagcagg ataaatagct
     2401 gagtaagtcc aagaattttc aacgaatgga ctaccaacaa atccattgtt tggattacca
     2461 tttggagatt taggaatcgt gtttaaaaat tgagttaaac tccaatttgt gatctttcct
     2521 ttatactgta atccttgtag caatgaagcc ccttcacagc cacttggagc gcctaaagca
     2581 tattgattgt aattctttac gccaagaagc ttatagtcgt cattgcttgg ttgtccattg
     2641 ccgactgcgt accaacctac gccttcataa ttccaacctt gcttactaat cacgtttttc
     2701 tcatgttcat ctagtgtgta gtggtgccaa tttactcgtt tattatataa gcgactaact
     2761 ggaataccat tagcaccttt ataagagtac cagccaattc cttcatacgt ccacccttgt
     2821 tttgtgatat tatcacgttc tttggcattt aaagtataga aatgttcccc attatagcga
     2881 ttatataagc gataaactgg atcgccactt gttggagctt gccaaccaat accttcgtaa
     2941 ttccaaccaa ttcttcttag attatctcgt tctccagtat cttgagtgta gaagtgttct
     3001 ccgctatttg gattatacat gcgatacata ggtattgttc ttactgcact acgagcaaac
     3061 cgactcattt gatcttgttg taactcttct tttggtgctt gagtcgcttc agcttcagac
     3121 tgtccttctt tcactggcgc ttctaatttt acttcttctc catttacaat tgctgtttgc
     3181 tcttcgctag aactattgct agtcactgtt tgctgcgtag tatcattccc tgtttcttct
     3241 gcaaaagcat tttgtgtagg gactgcccac gttgacaaaa tggttgcaca aattaataat
     3301 gtcagtttat tcttcatttt tatttagtcc tccatctctt tattaaaagt gtaacgatcg
     3361 tccttttagg gacttgatat tgacaataaa acgatatttg cttttcttta aatatttact
     3421 ttgcattaat gtacaacgta cgactctcac taaaacacag ttgtctttta gcatttgtaa
     3481 aacttgaact tcaaaagaac cttccaaact aggaacttcg caataataaa ttttaccgat
     3541 ttctatttct tgcattattt acgcttcttt ctaactggaa cttattacat tcctaaatct
     3601 ctttttactc gataaacagt ttttcttgta acccctgcat tctttgctat tttagaaata
     3661 gtttcttttt tcttcagacc atcaacaatt tcatcgtaaa tcaatttgtt ttttcctgtc
     3721 gcattggcat ggtattgaat ctttttacca gtataacgac catttttctt tgcaatttca
     3781 atgccttgtt tttgacgctc tttgattttt ttccgttcgt tttcagcaac atagctaaac
     3841 acttctatga ctaaatttgt tagcaagtct cttaatgcag ggtcagttac cccattaaac
     3901 gacggtaggt ctaatatttc aatagaagca cctttactac gaaacaattg aagtaagtct
     3961 tttgtatctt tactatttct tgctaatcga tcaagactag gaataataat cgtatctcgt
     4021 tcacgtatga aagaaataag ctcttgcagc tctggtctgt tttcaatatt ttttccagag
     4081 actttttcct cgaaaatttt taaatcgtct tcagcaatat tatttttgac tgaccagttt
     4141 tcaattagct tatgttgccg agctaaactt tgatcgtcgg aagaaactct taaataagct
     4201 aaatacattt gccatcattc ctatccaatc tatttactat tagtatacag gtcttagggt
     4261 acactttgca ataaagaaaa aacgctgaaa aatcaacgct ttaagcaaat atggatttgt
     4321 accattagag tatacccaaa agatacataa aaagagatag tttaatagaa agctggtttc
     4381 aagttgtagt cgttgctaac gacaccacct cctttaattc tttctcaaat atttaaaatg
     4441 tgaacaaaaa aataaaggtt agcttttatc aaaacaaaac tagcctttat ttcttttctt
     4501 ttttctctat ctacgttata ctataagtaa ataaggagct atgcgcgaac atagccccta
     4561 atgtgtagtc cgttcaaagc ggatggcatg tatataaagc tttagagcct gtctactctg
     4621 ccaaaagtta ggacggctct attttttttg ttcttttttg aactttaacc aagctaaaat
     4681 cgaatcgcta gctttcgcta cggcagtaat tactactgct gctgctccga taatagcaat
     4741 gatttgatcc aatgagaaca aatcaggcat ttcctttcta cagagtacgt ccgataagct
     4801 aataagccca taggcaccac gctccttcaa ttggatttgc caaaccgcca caaactttct
     4861 acagtaacat tatatcatag aataaagaac tttagatttt ttaaatatgt tgattatttt
     4921 atgaatggag atactcactt ttcggaatat atagtgtttt tagttattta tatttcgtgt
     4981 tccttcctct tctcttttgc atttttttgt ttagtttctt ctacttcttt ttcatacttc
     5041 tcgatcttgt ccgttagctt ttcttctcta tcaatcaaac gatacccact aagatcaaat
     5101 aatccatttc tatctagttc ttgcaatatt tcctgaacat caatcaatct atttaattta
     5161 ttttcataat gcttatagcc ctctataagt cgtttcctct gtttctcttg atttttcgcc
     5221 acagatatta tcttttcttt cgttaaatca ggacgtagat tatctgaagc tagtaatttc
     5281 ttttcagcaa atttaaattg gcgtacttct tctttgtact tttgttctaa tttaattttt
     5341 tgaagcattg tagcacgctc aatttcattc atgatcggta aatatttttc gtaagttact
     5401 aaaagttttg cgacattgtt ataatttttt atttttccat caatctcagc tattttttct
     5461 tttgcttggt ttttttgctc aacaatttca tttaatctct gttctaattg tgaagctgaa
     5521 actatttttt cttcacgaat cgtatttaaa gcttcgacta attgattagt atatcgtttc
     5581 cttgattgat aatgtagctg atcaaattta ggttttagaa acacatcaag taattcagga
     5641 gtttctttta aactactgat taatgaacgt ccagtaaatt tttgataagg taatttttta
     5701 tttgaattat catcgttctc agggagtggt tctgcatagt aattccactt ttccccaatt
     5761 aagatagaac aagatttact aatttcatcg attacaacat actcactagg aataaagagc
     5821 cgcttgatac tcagttctgg tatcgaaata gatacaccac gattactcat agataaaata
     5881 tttttgctag aaagttttat ctcttgtggc tgaatccctt gcattttttc cagtccacga
     5941 atgattcctt ccccttttac agtaaaacta gtttgttcgt ttgtctgttt atcagtaagt
     6001 gtataatcaa atttgtcatt gatccaaatt gtatattctc cagtttcttc atttacatca
     6061 acataattat ttgggacaaa cactaaacca tttaagtcgt catgttcgat agacactaac
     6121 aatccttctt tggtcgcttt ctctatctga atttcttgta attgaatagg cgtatcttct
     6181 tgaacaatca cacttttttc tttttcacga tatctttctt caattgaatt taaggaagag
     6241 tcattcgtaa actcatagga ttctctaaaa taatctttta gatttttaat ctgataacta
     6301 tctggcagca agctttcatt ccacttaaaa tcatttacat cattgaaatg gaaaacaaat
     6361 tgatcttgtt tatttaactg aaaggtaatt ccatgctctt gttccaaaag aaaactaaaa
     6421 tcttccaagt tgtcactaga aaagaaggtc tcttcaatcg cttctttcaa ctgattaatg
     6481 taggattgat tagcagaaag tttttgagaa atattacttt ctgtaaatgt ttctaagtca
     6541 tctaacttgt cccctctagt ccgtctttgt tgtccttcgg ataataaagc atacgaaata
     6601 tgtttccctt ttcgatcaat atttacccct aaatgatccg ctttttcttc aaattcctcc
     6661 catgaagttg attcatttaa gcacatgatc aatcgtttac gaatttcaga acgaaagctc
     6721 gtgttatttc ttctgttaga ataagatttg taggaatctt ttaatttttc ttttttcggt
     6781 gataaagata aatcatgttc agcacaaata cgattactaa tttctttaat tttatttgct
     6841 gttcgataag gttgacttct gaattttttc agatcataaa aagaaacact gttaaaaata
     6901 atgtgattat gaatatgttc ttgatcgatg tgagtggcaa tcacatattc aaactttcct
     6961 tcagtcaatt catcaatcaa ttgtcttcct aattcatgag caagctctgg tgtcactttg
     7021 tcttcaggcg aaaaactttg gattaagtgg tgcgctaaaa cctcactccc accgactttt
     7081 cgataatcgc cttttacttc acgtgcatat tccctagtca tttgcatttc aacttctgca
     7141 aattgacttt gacaattata actactaacc agcattccat tattcgtttt ttctggattt
     7201 gtaatatagc caattgcttt atttaaagta gtttttattt ggtgtatttt tgtcactgcc
     7261 attaaataaa cctctggtta tctctagcac gtcgcattct aaaaagtaat tcttcgttaa
     7321 ttaatttagt caattgatca taatttgttt ttaattctaa aaagtcttct tttgaaacac
     7381 tgtttgaaat attagctctg tgagctaatt gattgatatt atttccaatt cgtcctaatt
     7441 ctgtcgttaa attttttaaa tcagaaaaat ctgttgtaat cattaaacca cttaaacaca
     7501 taatacgagc gtattgggaa aaatttcttg tatttgcttg actcatttta taatttagta
     7561 gaaatatttc tctttctgaa aaacgtgttt ctttcctaaa cgttctttca gaatcatcat
     7621 aagtattggc tatttttaga atattttcag gagttttagc tagtttttgc tccatgtttc
     7681 cccctcctta ccaatatttt ttgttttttt cactagttta gtgttttatc tttgtttaaa
     7741 aacgaaaatt ttcactgtgt gaccgccctc cgattagaaa tcgaagattt caggggggcg
     7801 gtcttacagc aagatttgca agttaccggt aacttgcaca aaaaaaagaa aaatccgctt
     7861 gcgcgaatcc ttcttttttc attttggggc gctgccccaa tccccgtttc tattccaatt
     7921 cttcaatacc gcttagatca atttgcttaa attcatcttc actcattttt tcgagtttat
     7981 cataaacaga ctgataattt tttaatgtgt aatgtcgttg ttcatcatct tcaatatatt
     8041 gttttcctaa atcacttttt aacattgaaa gcatttctcc aacttcgctt tttatgacgt
     8101 ttaatttgtt tgcatttgaa cctgtaatct gtttgtgtcc gctaatgatt tctaattcgt
     8161 gttgagtaaa tgtttgcatg atttatatgt ccctctcttt tacatttcta ttaatttcat
     8221 ggattctttg atacttgtac aaggaaaatt attaattccc ttttcattca tatcacaagt
     8281 agtatagtat ggaactaatt tatttttttc ttcttggaca ataaaatatt gatcgttgtt
     8341 gatatgatta cagcaatata taccagtagc tggatattca acaacaataa aattattggg
     8401 ttcaaaattc tccttttgta gcattcttct aattagaaat aaatgatctt tttctagaca
     8461 atcaggtatt tcattaattc tttcttcagt taatgctcct tcaattatat tccattggtc
     8521 taaatattgt ttcattttta tatccctcat ttattataaa agttactttt tctgataaat
     8581 taaaaaagca aaatagatcg tttcagctac tttaaactat agtgtagaaa cgtctgtttg
     8641 ctcttttggc tggcgaattt tattaattaa tttttctttg tcacgttgtt catttaaaga
     8701 ttttgcttta tcttcaaatt cttctaattg atcagatagt gacaaagtac tttgtttttc
     8761 ttctttttct tttgtcactt ctttttgtaa ttctgctaaa ttcttgtcta ttttttgaat
     8821 taaattatct gcttgcttta aaatgacttc aaagctattt ttcaattcct ttaactcttt
     8881 atcactagac caagctgcta aatatggaaa gctatagtct tttgtatcta ttccataatg
     8941 ttcacaaaca acaaaagccg tactttcagc ttcaacttct tttgtagatt ttgtagtttt
     9001 tattgacgaa tttttatcaa attctggtgc gtgtaaatca gcatgagtga tctcatgaat
     9061 cagagttttt attgtttgcg cttgactcat accattattt aacacaattt tcttttctaa
     9121 tggagaacaa tatccttttg tgccaccttg tatatctgca aattctatag aaaaattggt
     9181 gctttgcttc aaggcaacat aaagctctgg ataatttttt acttctccac ttaattcatt
     9241 cattagttgc ggtaaaggtt ctcctgaagt ttgagaaaca tcaaaaactg aaacagggaa
     9301 aaatgaaata ccttcaactt ctttttcttc tttttgccca ttctttattt cttctacttt
     9361 cctcttatag ggacgaccac caattatttt aatggatttt tctcctttat ttacttgtct
     9421 attaaaagag tttttccatg ttttgtaacc agcaacatga gtagcattag gattttgcat
     9481 aagaattaaa agggtattat ttaagctgta attatgaaac ttagacataa cacgaagaaa
     9541 atttttgtat ttgtctgaag ataatacttc tttaatacct tcttctgcct gtgaaagtaa
     9601 ttcagcggtt ttattagttt tttttgcttc actatcttta taagacattt atttcattcc
     9661 tcacttttat ttttgaatat cttcaatttc ggattggtta ctattttgat tagtaactgg
     9721 taatgttttt tcgctaacct gttcattttc tattttgttt tgagaaacac tagcattatc
     9781 tatgttttgt ggttggcttg ttgaatcttg attttgatct gtattttctg gaatgctagg
     9841 ttcttcactg tcactcggct ttggcaaatt gttaaaatcg ttcatcacat cggcaacatt
     9901 attattttgt tcttcagctt gattattgat atcaacagtt tgctccttag cgctagcaat
     9961 atcagtttgg ctttcaattg gatcaacttg attcaactgt tctccaacag tcttattagt
    10021 ttcatttagt tgctcattag attgtgcagc ttgatttatc tcatcttgaa cagctgaagc
    10081 aacttgttcg ttggattgtg catggttaat agccgaattg acgttagaat ccacctcaga
    10141 aacatctttc tgtttatttt gatattcttt tttagtttct tctaaattat ttagcgtatc
    10201 agttgcttgt tctacatttt tatttatttc gtttgtcgta ttatttgctt ctttttgtgc
    10261 agtttttact tcagtgactt tttggtttag tttttcatac gtttctttat aaaggtcata
    10321 attgattgtc aatactgttc cgtcttcttc aattaatttc ttgatctcat ttgtaagttg
    10381 attatattca ttgactaagt cagcaacttt ttgccattgt aatgctgctt tatccacgtt
    10441 cttttcagta cttgctccaa tactttgtaa gttatccaag ctatctttta cttgagttag
    10501 ttgtttattt aattcatcag cctgtttcct agaatcattg agttcttcct tagattgatt
    10561 aatttgttca tcaattgaag gttctggatc tggtgttgga tcaggctctg gatcagggtc
    10621 tggatctggt gttggtattg gatttgggtc tggactaggt gttggatcag gcaatggatt
    10681 tggctctggt acaggcaatg gatcaggtaa tgaattatca ggtttagtat catttccttt
    10741 atctaattcg ctattttcat ctttattatt ttctgtttta ccgttctctt tatttgaagg
    10801 tttagtatcc tcaagttgag gtttataact caaatcaact agtactggct catctacaga
    10861 ttttttgtct ttaataatta atttttcttg tctgtctgcc tcttttttag tctgatcatc
    10921 taaagctact gctaatctat caattagatt atttttgttt tgattaggtt gttgtttaat
    10981 tagcggtgta tcagcaataa cagccaatat atcatcagct ttctttgtag aatgtttgtt
    11041 tttttccttt ttgtcttctt tgcctttcac actttcttcc catgctgatt tcttactttt
    11101 gtgtttgctg gactctgatt tagttgtttg ttctgtcttt tcagtctcat tgtgtggttg
    11161 ttgagaatac atgccataag taactgcacc ccctaataaa acagctgaaa atagtcccac
    11221 tactaaaaat ttctttttgt tatgcattat cgtttcctcc tagttgtttt aagttttctg
    11281 accacaattt gtaattgttc tcagcttctt ttctttcatc ttctgataat tttgaatccg
    11341 atctgtcctt ttcatatttt tgaagtagat taaccatact tttagctact ttaatatctt
    11401 cggatagttc agaatcattt atttctttat ttattttctc tgccttctcg atatcttttt
    11461 catgcagagc tttatatgct tcttgctttt taaattgata aatttgatct tttaatgttt
    11521 gattatcaat ttctttgtta attagttctg cttcgtctag ttttccttgt tttaagtatg
    11581 cataaccacg catagcttgt attgtttcgt ttggtgtaac atttttcatc gtagttactg
    11641 cttgccaatg ctcattcaag aaatcccaat aaaaaagagc tatctgtgaa tcagatttct
    11701 ctgccaattc ttttaattcc ttagaatttt tttcgctata cagtttttct actaattcgt
    11761 tttctttttc aggatattgt tttattattt tttcataatc tttcttcttt aacagctcat
    11821 gatagctttg agaatcagta gaagttgatg aatgattatt taatacaaaa aaacttgtga
    11881 aaaataggaa cagaactatt atcaaagcca tgtagatttt ttttgctaaa ttagtagtaa
    11941 ataaaaagaa gaccgtctga taatttacgg tcttcttttt attctttatt tttggttctt
    12001 ctatatcatt caatatcatt tgcgacttag aagaactttg ctttttattt gaggtaggtt
    12061 caattaaaag gttagtcaaa atagtctcta taccttgttc tattggtaat tggattagtc
    12121 gtctatttag ttcatttccc tttttatcgg ttaataatat taatgtagaa cctttagtat
    12181 gcttgtctgt tagccgatcg ttttcctgtt caagtaaata aatacccctg taaatttcta
    12241 caggggtatc tggcaattca atatctcttt gttctataaa ctgaacatat tttttctttg
    12301 aaaacatatt tttccccctt gaatgtttta ttcttcagct tcttcagttt cttcagcccc
    12361 ccatagaaat tttgggatag aaaggaaaaa tgaactaaca attccaagaa aattaggaat
    12421 tttatctgag ataaaagtaa aaatagatag tattaatgta aggattaata tacctatagt
    12481 aggcactgta aacatactaa tcgtttcaga gaagcctaaa gaacttaatc cataaataaa
    12541 cagacctata aaaccaatcc atactaatgt aataaaaagg atcacaactt ttttaagaat
    12601 ctgtataaca agcaaaatag gtatggaaat tatgttaacg gcaataacaa ttatggaaag
    12661 taaaaagcaa ggaattgcta ctatacttgc taatattctt ttagctatta acatactttc
    12721 accctcttat tacttgttta tcttattata tcgcttatta tctcaattaa aaagatatat
    12781 atttaattaa ttatggttga taaaattatt gttatcaggc tgtttactta gtattttatt
    12841 caaatctttc acactaagct tatggttatc aagaatcgct aaaacctgtt ctgcttctaa
    12901 atctctaatt tgctttgtta gtttttctag cttatattga tcttcttgaa tcctcttttc
    12961 aagctttgtt gcttcttctt tcttctgctg aatacgttct gttaagctct ttcttgccat
    13021 cagaaatcat ctccttttga cataactaat gtttttattt tacttatatc tgtacctaca
    13081 taacgctgct taagggaaat gattgtagga acggaagtca aaccatactc cttaatataa
    13141 cgtttatttt ctttgttatt catgttaaca aaagttaagt catggtataa aaaattgtga
    13201 cgtatcaggt aaggcatgat cttttcacag tctctacaat cgtttctgta gaaaactatt
    13261 ttctcttgat ttgattcatc taaatagact ggatcatttt gtgcttctat atatttgata
    13321 taagaatgat ctgctaaaaa tatgatagac acagtaaaaa ttaagacaca aatagaaaaa
    13381 atttttttat tcaatcatat ctccccttaa aactgacccc aagctttgta ttttagtcaa
    13441 tttcttcgca tgaatatcat actgaacagt atagatttct gaatttttaa accagtcagt
    13501 ttctcctaat ttactttcat agtcaacaat caaaagacca gtaatagttt tttggttaat
    13561 taatgaacca ctatatatct ctaaattatc taatcgagaa ctcacattat tagaatctcc
    13621 ataagttagc tgattctccg aaaatttctt atacaactct tcagaaatca aatctcttac
    13681 gttttcctta ctttctttgt atgttttggg atcataatta aatcttgact taaagtattt
    13741 agtagcaatg tttgtaaatt ctttgtagac atcaccatct ttattgacta tttgtgtttc
    13801 aatcatttta ttattttcat cttcaagttt actaattcgg ctcttttggt ctttcgtttc
    13861 tctttttaaa atatcgtttt tcttttctaa agcagctatt ttagaatttg cagtttttac
    13921 agttaactct tgcttaatat ttaatccaat ggataaagaa aatactgata cgatcaatac
    13981 aaatatagtt atagtagctg attttctgtt gagcaaagta cttcctcccc tctaattatt
    14041 tttttatagg acgtgctaat acaacatcgc caccatctaa taagctcata aaggacgtac
    14101 caaaagcttc aatatttaca ctatcgccat ttccaccttc ttgaatgatt ttggtatctt
    14161 tacctctcca cttttcagtc aaaattcctg tgtgtccatt actacctgct ccagcacctt
    14221 gattaacaat aacaatatcg cctgcttttg cttctgtctc tgaaatttgc ttcaaatatt
    14281 ggtgtgatcc tctagcgtca cttgccattg atccagtaaa ccattgcata tttgcaggta
    14341 ctttataccc agctttattt aaaactagcc aaacaaaacc agagcaatca gttcttccat
    14401 ttttattagg attttttaaa tctgttccta aatcaggagc agggtgtatc tgatcatagt
    14461 gaaaccaacc tagccaactc ttagcaattt ccataatatc gccaccatca gattctaaat
    14521 cacaagtaag cgtatttaat tcgctatctg caccattact aatagtacct tcagcaattg
    14581 gatcactcgt ccctaaagtt gcgtaccaat gatcagcata agagtttctt tgatccaaat
    14641 accattggct agagtcttga gataatcctt caaacttcat gacccactct ttgcatgctt
    14701 catgaacatc tgtcattgct aatatttttt tgctagattg ataatatgaa ctatttaatt
    14761 cagataacag aaattctact tgaacaccta aatctgtttc cttctttcct tttttgtgtg
    14821 caaattcttt taaagaggtt aaacgagaac ctaaccactg ggcaattcct gtcgcacctg
    14881 agcttggatt aactgcactt ggattcaatt gagattcaaa ttgcatagct cctaatgctc
    14941 cagaaatccc ttgaggggtg gactcaggta tttctttacg aataattgag taaatacttt
    15001 tagcatttga ctctatatct ttggtagtta tattgtcata gttggcacta gtatcacagt
    15061 cgcctgctcc gaaattaaaa atgttaacag ttgaaattac agccgtaaca attgataata
    15121 taagcactgc taaagttagt ggaatcgcta atttagcaat gagtgctttt gctacttctt
    15181 ttgataaaac ttgttgaact ctacgtatgc ctatttcagc aattctttgt gtatatttgt
    15241 aacttgattt accaaatttt gttagtgctt tactactttt tttagcagta tttgctgctt
    15301 tttttgtttt tttagcacca ctgatacctg tatcaatgaa atcttttaat tgaatcgctt
    15361 cattaatccc ttgagattct gaatcacctc taaactcttg gatttcattt attactgtat
    15421 cttttgcagc tttgcttgtg tttacgagac cttttttact tcctttttta acaacagtta
    15481 aaacttcttt ttttgaagcc tttgtttgaa tcaattcttt acttttttta gtaactttag
    15541 ttgtagtttt agccactgtt tttgttgcct tagtgctatt ttttatccca cgtcctattg
    15601 ttttactcgt ttctaattca gcagttgcat tcccctgttc aacaccattt ttttgtgctt
    15661 tagagttaac atctttgact acattttttg ctgaatcttt ttttattaaa taggaagtgc
    15721 cccgactact ttttattttt ccactatgat tagctttctt agctcgtttt gtatagtcag
    15781 tagatacaat ttttttcttg aactgattat tatctcgatt tactacaaac ttttttgctg
    15841 tcttttgttt tcggataatc cgttctcgaa aatctgtgtt catactgtag taccttgttc
    15901 tctttgctca ttttgatact tatacacgtc ctcaatctta gttgacatca ctttatataa
    15961 gttagtatct tttggaaact catcataaag tggaatcata gaatcgcccg catacatcaa
    16021 tcctgcacct tctttggcat tcgtaatata ttttttctga cggctagaaa tgtgtaacaa
    16081 ttcgcctagt ctttctcgat cacgttggga ttgatttaac attagaacaa aatcactatt
    16141 tgatagcata cgttctgcat ttaatgaaga taggagcgta gatacattct gtgtgatacc
    16201 tgtaggaatt gctccccatt ttcttgctct tgaccataat tcaaaaaagt aattctctgc
    16261 atattcattt gaaaaaagaa gctgaatttc gtcaatatat acccatgtcc gacgtcctgc
    16321 atttcgatta cttgtgatac gattccaaat ttgatctaga ataatcaaca tgcccatcga
    16381 tcgcaattgt tttcctaaat cttttacatc aaatacgata aaacgtttgc tcgtatcaac
    16441 attggtttgg tgggaaaata ctgataatga tcctacagta taaagttcaa gctctactgc
    16501 taattgtctt gcttcatctt caggctgatc cttaagaata ttagaaaaat cttttaaagt
    16561 aggcattcca gacccatttt caaaatattt cttgtaggca aatcggcatg aacgatcaat
    16621 taaagttttt tgagtcggac taagaccaat attcccccct accaacattt cgcaaagaga
    16681 taacacaaag tcagtcttta aaatgactgg atcatcatca tctgcatagt cattattaat
    16741 ttccatcgga ttcacatgat tttttgatcc tgcggatatc tgaataacct ctccatttaa
    16801 agcttctgct aaaggtgtat attctctttc aggatcaatt actattactt cagcatcggg
    16861 atcactcagt aaaacagctg tcatttcttt tttagtagca aaagattttc cactacccgg
    16921 agtacctaaa ataaaaccat taggagcttt tagttttctc ctatctaacg tcactaagtt
    16981 ttttgttact gagtttaaac cgtagtattt cccatttggc tgcataagtt cttgtgcagt
    17041 aaaaggaata aatacagcag tactagcagt tgttaatgtt cgatgtatag ggacaaaatt
    17101 gtttccccat aaaagcgtag agtttagacc ttcttcttga agataattta actttgcaaa
    17161 ctcgcagtca tttttccgac ctgcagttaa aatttgctca attgtgtcat caagctttct
    17221 ttcatcttta tcaaccgcca tcactaaaaa agtaaaagaa aagagctttt cattgtctcc
    17281 ttgcatttga tccaacaatt cttgtgcttc gtccatactt cttcttaatt ccattggtat
    17341 caagttagga tcataattac ttctagccgc ttttttctgt tcatcaatct tttgttgctc
    17401 cataaatgat aattttgttt ttactaaatc taatcccttt tgttgatcta aaggctcaat
    17461 atgcaaagta atctgtaaat tagtagggat atctgtgata ttagtaatta atttatccga
    17521 aagttcagca ggataattac gaagatacaa aacttgaccg taataatccc ccatcttgaa
    17581 acgatcctta cgatatttaa aatcaaaact atctggcgca attgctgaat gtgtagttaa
    17641 gccactgaaa agtaaatcct catattcaaa atgaagtttt ttattgggac gtagaatatc
    17701 attgacaacc cttagacgct caattcctga cagttcttct acttcacacc ccatttcgct
    17761 aaattgatct tctaaatcgc cttgaagtct atttaaagct ggtatagctt gttctaaagt
    17821 ttttgctctt tgattaaaag taatatattt ttctttttgt aaactactat tcccatattt
    17881 aatcttatta gatatcatat gattcatttc ttttcgatat acatctagtc catcatcaac
    17941 taattcgtaa gataagtctt tttcaaattg tttcttgtct ttacttctat taataatcga
    18001 aatttgcaaa gaagtatcag aatcacaact atttagtaac tcacaatagg agctaaaagt
    18061 taattcttgc tcagattgat ctgcaattgc ataattgata tctgaaaatt tgaatgtacg
    18121 agaataaagc ccttctttaa gctcacacac tccattctca tacattgctt tatatgttaa
    18181 tgatcgttgg ctagataaag gtggagcgtc tctacgttga ttttttccac gctgtttttt
    18241 ctgctttgct aagattttta atactttttt tgcttgctta tcttttggtt gtttctttga
    18301 ctttttggaa tcgttttttt ctaaaatcat ttagattgct cctttcttta gtctgatata
    18361 ttcttattcc agttttcgag taataattta gaataatctt gccatatttt tctaaaggca
    18421 taccttcttt tcttatccag ccaaatgcta caataggtat caatagtggg ataactatat
    18481 aaccggctgc gtcaaccgac atatgcaata ccaatacaca atatgctgtt actcctactg
    18541 agattaatac tgccaataca aaaaatacta actgcctaac gctcattcct cccattaatt
    18601 tttcttgata atccttaatt tcttttggta cttttactct aattgccact caaacaccta
    18661 cttttctttc ttctgtgaaa aaatatcaga tattaatttc ttaaaatctc gcaacatctc
    18721 ttttgtaata attacgatca ttatgagtgt aaaaacaaga gaaacaataa aaaatacata
    18781 aagtatactt agtgttaatg gatttgaaat atcataagtt gttaataata tggatagtat
    18841 ttgcaaaaaa acaaatagca ctccaagaat tagagtgctg ttacaacgag attttttcat
    18901 tgaaaagacc ttcctacatt gcatttgtaa tggatttaga ccatcttcca gtcgccatta
    18961 ctgcgaaaat tagtaataca ctattaccaa ttatcccaat cagtgtcatt aagaatgttt
    19021 cacttgctga accactattt gttatatcac tgaaagccga acttaagaga tatggataaa
    19081 actgcataac cataaatatt aaagttccct gtaacgctgt tgctgtaaat gattttagaa
    19141 agtttttacc tatactcgac cattcatcat taggaaatgt cgcaattgga attggagaca
    19201 tagctaagaa tgcgtatagt tcaataaaac gcatatttac aactacctgt acgattacta
    19261 tcgaaccaat agtaattagt aatgcgataa ttaaaacagt cgatacacct actttaaaca
    19321 ttagtccttc ttttaaaatg ggagctaata ttttttttgc gtccagcata tcgcttactt
    19381 ccttgtttcc catacctaaa tgattaatct tatcagtcat atatcctgta atatccataa
    19441 tcgctcgcat aattaaagat acttgatcca ttgctagttt acatagacct agtcgcacta
    19501 aggatttaaa aatcatttca aaaccagttt gacctccacc attagtctcc acacgattag
    19561 aaattctttg taattctaaa gcaaaaaaca aagccaatat gctgaaagct attggctttg
    19621 agacagaatc aagaatagtt gttgaatatt tgtataactg aggactaaag ttttctaagt
    19681 tgctagttaa gtggcttgtc ttactactaa aggagattac ttctaaaata gattcgacac
    19741 accatgttaa aaatccgtcc atcattcatc cccttttcat taaagactat cctctaaaca
    19801 attgtcttaa ataacgccgc tgctgctaca attaaagctc caccaacgat ttgccaaatg
    19861 cctgattgca tttgtggacc attgtggtct tttaatgcac cacctaaaac aactgcaccc
    19921 catactgcat ataagcctcc gcctacaata gcgtatttgg acaaaatatc tataatagcg
    19981 ttaaatgttc ccattaatta ttctctcctt ttataattca tctaaattta ttgtttcaat
    20041 ttcttcgtct tcaaaatcat aagtttcgat ttccgtatgt ttcaaaaact ccttcatatc
    20101 gacatttttt gataattcaa aatcaattgg atcattattt gcaataaatt cttcttgatc
    20161 caacggtgat tgatacatat aacctgaagg tgtctctttt aactttttaa ataaagggtg
    20221 gcgttccaac ttaaaacgat cagataaaaa aggctttgtg ccacgtaaca tataaataca
    20281 ttttccgcct ttaatctcac gcacttcatt tggatcaagt aggtttcttc cctgcttttg
    20341 agagttttta gtaaaacttc ccgtagttcc ccttgattcg ttataagtta ctacatcaat
    20401 tgtttctttt cctaattgag ttgaaatata cttatgagta ctttgctcgt ttcctcctaa
    20461 atacaaaaaa gaatcacagt ttcctatgat tgtttcccaa ctgtctttat aaagcttttt
    20521 actaatctgt gttaaatttt gcaagataac atcaactgaa attcctctac tacgtgttgt
    20581 tgcaataaca ttttcaaaat ctggaatttg tccagtatta gcaaactcgt ctaacaaaca
    20641 ccttacagga attggtaacg atccattata aacattatct gctttataaa ctaacgtatc
    20701 aaaaagttgt tggtaaatca tacttgctag aaagttcata gtagtatgag tatctggcaa
    20761 aataatataa agtacagttt tacgatctcc tattgtatct aattctaagg tgtccgttga
    20821 cgttaatttt ctgatatctg gaacgtcaaa aggagacaaa cgaacaccta aactaattaa
    20881 aatactctta gccgtctttc ctgctgccat cttaaaattc tgatactgtt ttgctgcaaa
    20941 acaactaggc tgttcttgct ctaattcttg aaatactaaa tcaatcgaag aaatatattc
    21001 ttcatcatct tcttttactc ttgcgcgtct tactaagtct gctactatcc ctaaattttg
    21061 ttcttcaggt tttcctttca aaatcaacaa atggaaaata gctgccaaaa aagatctttg
    21121 tgcatcgtcc caaaatgaat ctccaccctt tttgttaggg tctgaagtat tcttcattag
    21181 attgttaact actttcaata tatcaatttc acaatctcct tctgttcttc taatataatg
    21241 gaagggatta taaccatcag aatttttcat ggtattcaga ttaaatactt ttatccgata
    21301 cccattttgt tgcataatgg cagctgtatc actcaagatt ccacctttgg agtcattaat
    21361 aacatagctt gagtgcattt gtaataaatt gggcaataca taaaatctag ttttacctga
    21421 accagctcca cctacaacta caacgttatt attacgttca tattccttat tcttttttaa
    21481 cttagtttcg atcgttattc tttctgtatt tgtaaataaa agattatttt taaatttttt
    21541 atctacataa ggagcaatgt ctttagaagt accccatttg gcactcccat gttcttcgcc
    21601 cattcttaaa tttttcttgc cacttataat atataaatca atcagtaaaa caataaccgc
    21661 aattatcgcc ccacctttca tagcaatttt tgaaaaagta aatggagaaa tggtaaatgt
    21721 cttaggttct aagaacttat caaatgctgc atttactaca tttatccatg aagaatctgt
    21781 attttgagga atcgagaaat aaaccataaa aaataaagta cctaaataaa agaaaaaagc
    21841 agcaagtaca ataccaaaaa caaggtcttt tttggtaata ttcatcgttc ttgcccctta
    21901 gttttatttt tcgatttctc ttttagttgt tctctttgtt gttctctttg ctgatttatt
    21961 tcttgcgctt tatcctctaa tttatctaat ttttcagcta acgatttatc tttatcctta
    22021 ccgtcagcag aagatttttc aatatcttta ttagatttat cctgctgttg ttctctattc
    22081 tgatcttttt cattagcaaa tttttgtagc acattttcta atgctaaatt gagagtatct
    22141 ctatcttgag ctttaaagaa aacatgtatt tttccagtct ctttatttct ttttacggca
    22201 aaatctacct tatatttctt tagttcattt gcaagtggct ttaaatctgt ctttgaaaca
    22261 tcaatatgtt ctaacgatcc atgctttttc aattgattat agcgctgttt tcctgtagtt
    22321 ctctgctttt taatttgtga atgaactgct ttagctggga cagtaacaac tgctatcccc
    22381 gctttgctta gcccctgtaa caatcctcgc attaaatttt tgccttcatc caatacaaac
    22441 tcagccacac gttcatcatt tactgccaat ttaacaacct cctctttaaa ataaccttga
    22501 accatattat cagaaacaaa ttcatattta agttgttcat atcctgctcg ttgcatgatt
    22561 ttatttcgag atattctaaa actttcatag ccttttatat attgatcacg ctcattgaaa
    22621 taagctttcg ctttgttctt tgcgtatctc tccatgaatc gtaaatcatt tttttcaaaa
    22681 aaaattgggg acagtatttt ttcaagcttt aattgttgta tatgatagtg aacaaagttg
    22741 tctacactta attcgtcatt cacacgtcct tcatttcttc tatcaaacat cggcaaacga
    22801 ttgacaccaa atgacgtaat ctcttcttgt agaaagttat aatcaggtgg acggtgattt
    22861 ttttggtaaa aagaatacca cttagtttcc gctcgaatca gttccatatt aaaaagttgt
    22921 cttacccgat tatctttacg atcaataaaa gtcatatttt ttaataaaaa tatgacttgg
    22981 aatttatatt cttctaaata atcttcgagt tctataggtt cgcctaattc ttttactgtt
    23041 atattcatag atatatcctc acataagctt tttacctacg tttttttacg ttgtaaggca
    23101 aaattagttt ctctttagga attttgtctt cttctgacac gaaaaattca aagtatcctt
    23161 gctcatatac atttttgttt cctcttttat tgctagttct ttcttgatca atcattactt
    23221 cctcataaac tgcgtctcca ctaataactt gctttccacc tactactgaa ataaaagtct
    23281 tatagttact ttttgtcact aaggctactg ttgaaacata aaatatactt tcttcattga
    23341 ctactttatt tccgaaaagt tctgcaactt tattaatgga tagacgaata cttgataagt
    23401 tattctctga atcaaaattt aacttaactt ctttaccaat agtcaagtag atagtgattc
    23461 cttcatttct attcgtcata aaatttgtac tccctctttc ttaattatct tctttcatca
    23521 atcatatctt cataacgatc gattgctact aatgacatta acgccacacc aataaatccg
    23581 cctaaaaata aaccacttat aaatagcaac atagacaata cctccttaaa aattgaataa
    23641 aaaaaacagc cttattttta aaataagact gtttctggat caaccaattg tgattgactt
    23701 aaactatgat ctaaacataa ttcaaaatgt aaatggctac ctgtgctatt tcctgtcgag
    23761 ccaacatagc caatgatctg accttgtttt actttgtcgc caactttgac ttgatattct
    23821 tgttggtggg catataaagc cgttgtaccg tcttcatgct caatagctac ataattcccc
    23881 catgaagggt gaaactccgc tttaacgacc gttcctgctt tgctggcata gattggctcg
    23941 ccttgtgctg ctgccaaatc taatcctcta tgaaattctc ccccacgagt accaaacgga
    24001 ctagaaatcg tatagttttt cactggtaca atatatcctt ctttacttaa ttgtggtcct
    24061 gcgacttcct tgtcgtagtc cgttaaatga taagtctcga tcaaaccatt taatttttga
    24121 tcgtatttcg tatcagttgc atatctgcca gttaagaatt tggttgcttc ttgataagtt
    24181 tttgcgtttg ttttccatac accactataa aattgactat taccagtcaa accttcttta
    24241 agcagctgtg catagtcatt taagctatcc tcatagtttt catactgtct aaaagtagct
    24301 tgtgtggtgt aaaaagtgcc gtcacctaaa tcctcttgtg tggcaaaaga aacacctttt
    24361 ccgttatgtg ttcctttgat tccgaacaag ttataatttg gcgcttgtgc taactgactt
    24421 tgtccgctag ctgattccaa aatggcttgc gcaatcatga cagaagcgta taagtcgttc
    24481 tcctgtccta ctttgcgagc agattcgccg atttttcgga caaagctttc tacggactca
    24541 tctttttcaa aatgaatgtt tccttcagcc gtttctttgc ttgaaggaac taattcttga
    24601 ataggcgcag ttggcgtttg gtgatacggt tttgactcgt tttctgctgg tttagcttgc
    24661 tgtttttcat ttgttgcctt tgcttgattg tttggagcaa cagacggtgc ttgctgattg
    24721 tttgaaggtt cagttgttgg ctgagtctgt gattcattcg tagaactgcc agtgttcggc
    24781 ttttctggtt tcgattcact cggctttgtt tcgctaggtt tggtttcatc tggtttagac
    24841 tcactactgg attccgttgt acttgttgaa tctgtcgtac tgtcttctgt aggcttttca
    24901 gacgattctg ttgtagacgt cgtatctgtc gagctagttg ttgtatcatc ggttgtactt
    24961 tgatctgtac tactttctgt agaatcagtc gctgtttgcg tagtagacgt tcctgtttgt
    25021 tcatcactac ttgaagtaga ttcttcagac tgattattca tcgtgctaga ttgactggtt
    25081 ggcgttgaaa gctgttcatc tgccaatact tgtaacggtg ttgtcaatag catttgagaa
    25141 agaagaacag cggaagctac ttttgaaata tgcttttttt tcactttttc tctttccttt
    25201 cctgtattac atctaagata actttatttt atgaattttt tataaaaata tatataattt
    25261 ttgtcactca ttacactctg ttaaatagaa tacaacttga gagacccaca agaaaaacta
    25321 acccaatcag aataggataa gcattagcta attgaaatat taggatagaa aacacttgga
    25381 agttagaata aatagctttc ttttctgtga agcaccattc tattttattt gtttgctttg
    25441 cctttttagt atccttatcg ggagtaatac tttcctttgc tgcttccgtt ttcttagttg
    25501 aagtcgcctg ttgatcaatt agacgttgtt tattaactgt cttatttctc gttgtttcca
    25561 atcctaaagc ttgtttaata tcatgtgaac cattttcagc tgagtaagat cgaacatacc
    25621 tagcttgaat taatgcacga ttagctgtgt gtaatcctcc ctcacatcta aaaatagtca
    25681 ttaaaggcga atcgccttct tgtgggatat ccaataactt accctctgat tggttaacaa
    25741 ttttatttgt agtgatctca tattcataaa tcttgctaaa atcagtcgca taaattgttg
    25801 atcctacgct agattgtggt aaattatgaa gaactccccc accttgaact aaattatgtg
    25861 ccatcaaaac gtagtttcct tttccaagta actggtttgg ataataggtt cctaagccaa
    25921 ccattaaatt gtcattgctc attcctgcaa gaatcttgga gtatatacca gaagacggaa
    25981 tatagagaga accaattccc cactggttaa caagatcctc gtagctcagc tgtgcttccg
    26041 catactctgt aggcgtaaca ggttggattt gattttcatt gtatgttgct ttccctattg
    26101 tcgtttgttg tatctccttg atttctttct ctgactgtat agggtgagtg atttgctgtg
    26161 cctgtttaat tgtattttcg ggacgaagaa gaacggtata accataaaat ccacagattg
    26221 ctaaaatcaa gctactaagt agccacaata acggtaaggt tttaatgatt ttctttacta
    26281 tattgatctc tcctttccct aaaaacaata actaaaatag gaaaagcacc tttttcaaag
    26341 tgcttcccct tcattcttat ttttcgtttt tatttacttt tttcttagtt gtaacataca
    26401 atgctgcacc tgcaacagca gcaattccta gaacgattaa gcttccaata ttaatcaatg
    26461 atccagaagt ttgtggaatt gatttttgtg gtgttgttgg agttgctggc gtaactggtg
    26521 tttttgccgt tagtgtttgt ttttcatttt tcaaatcaaa gtgtttggca tattcgtttt
    26581 tgtgttcttt gtctgtataa gcaatatgag tcgcaactaa tttttcgccg tctttcaaag
    26641 tattcgcttt gtagttataa accgctttga actcataagt catttcgcct gctttgtgtt
    26701 ctgattcgtc cgtacctaca acagtaactt ttccattttt atcaatttta tggaattgtg
    26761 ttacatagta gaatgtttga ccgatatctt ctttaggtac agttgctttt acttcatcaa
    26821 ttaatgtttg atctttcgtt ggatcaaatg tttttccacc atcttttgtt acgaataatg
    26881 tttcaatagt tggcaattca ggcacgactg gacgaatcgt ttgttcttta ttcgttaagt
    26941 cgttgtgttc tgcaactttt gctccatctt taatgaataa ttcttccgta aatacgtaag
    27001 agacagtatt gtcatctttt gaagtatcta ccatttgctt aataacgtct tctgctgtat
    27061 cgccttcagc tacaaaatca tgagaacctt cccaaacaac tttagcattg tcatagtcgt
    27121 cgttaacgat tctccaaagt ttgattttgt ttgtatattc gacacctttt tcaacgttag
    27181 taattgtagc aacgtcttgc attggtgttt cttttgaagg tgtaaatgtt ttttctccgt
    27241 caacagtagc ttttgtgcgg atagttggca tttctttaac tttattagtt aagaataatt
    27301 tgcttaaatg aatcacattt gaaccttcat tgattttagc ttctggtaca gtcaatgttt
    27361 taattggttc tttttgtccg tcttcagtaa tggtaaatgt gtattcacgt ttttcagcat
    27421 caaatttgct gttaatgatt aaatctttaa ttgttttgta gccttctgga gcttttactt
    27481 ctgacatttt gtagtcgcca taagggactt cattgaatgc ccagtatcca tcatagccgt
    27541 tttcatctgt ttcagttgtt actttacgtt gttcgccttt tgttggatca ataggagtta
    27601 acgtgaagtc aattccattg taaccagtct tagtatcgcc tgattttgaa tcaacatatt
    27661 taaagccatc aagactaaat ttgattacat tttcttgtga agtgctttga gtttcgatta
    27721 cttttgtctc ttgatctttg taagtaatag aaaattcacg tttcgtatta tcaatttggt
    27781 atccttctgg agcaacaagt tcttgccagt agtagtcacc taatgctaag tgttcaacac
    27841 ttgcttgttg ttcttcgtca gataattcaa atttcacttc gtcgccttct aatttgtttc
    27901 ctttagaagt tgttggtttg aagttttcag accacttaac aggagtaccg tctttatgga
    27961 ataaaccgta ctgcgcccca tcaaaagtag cacgtccttg tgtttctttg cctgtttcag
    28021 cgtcttcttt cgttaacaat gttgaaccaa caacttcttg gttcgttcct tcagcgcctt
    28081 ttacaattac tgctgttgtt tggtttgcat attcgatttt cactgttact ggtttgaatg
    28141 tgttagcaaa cccattagaa gctgtttttt cagtaattac ataagtacct aatggcaaat
    28201 cttttcctgt ttctgcatga cctttttcgt ccgttgtaat agtgcgaaca actgcccctg
    28261 tctcagttcc ttcatgaatt tcaaatacat ttcctgctaa tgtatagtta gcattccaca
    28321 tatcttttcc tgtttctaca ccagattttt caatcaggat ttgtccttta gctttttggt
    28381 tacgttgtgt taaagtaact gtttgtcctg cgacaacatc gccttctact acgtcagaat
    28441 cgccaatagc tgatcctaaa acataaggag ctggtacagc aatttctgtt gctttgacat
    28501 gcacaccatg attaattcct ttgatcgttg ctaaaccatt tttatcagta gtaacagttt
    28561 gtgctgaagg cgctccttta ccactaaact ctactttaaa ttgcgtatta ggaacaactt
    28621 cgccagtttc tttatctact tttttaattg taatatctcc aactgtttga atacggaaat
    28681 tcacgctgta gttattaggg tcttttacac ttggtaagta gactgtttgt gagcctgttt
    28741 tttctaatgc gattggtgtt ccttccatgt atgaacgttt aaaagaaatg cttccacctg
    28801 tcacattagg atcagtagga gttacatcga ctgatttacc atcagctgca atgttccagt
    28861 taacttttgc actattagaa acttgagttt caaaagaatt taaatctaca gaagaagtta
    28921 attttttagt ttcgccaaat gttagatcaa tcgtcttgtt gttaaattct ggcaaacgtt
    28981 tatattcttt gattccatca taaagttttt gctttttagc ctgaaaatca ggaataccac
    29041 tgatagagtc aactttgatt ccatattgtg gtaataattc ccaaatgaca gcttgtgtga
    29101 caattcgatc ttcattggta aagttagttc caaaagcata aaacgtattc caaatgacag
    29161 aagcaatttg tgcgtcttta ggaatgtcag ttacagcaac tgcttcataa tctggattat
    29221 ttgtagtaat aaaagggaca ccgggctgaa tacaaaatac aattctatca gtagccccat
    29281 tattaggaga aactgccatt gcagctgttg cgtcgctcca tggttgccca tgcttcgtac
    29341 catgcatagt ataaacatta cttgcatcat aatagatatc gcctttttct cccggcttca
    29401 tcgtaatatt atcggcattt acactttgtg aactaagtcc aaagcctaaa aacattgcca
    29461 atgcacctaa aatcatcaat agatacttgg gaactttcca cttatttctc ttgtttttca
    29521 ttttcacaat attctccctc tcttcaatca atatataagt acagtaaaaa aagagcgcca
    29581 cccaaaggct cgctccaccg actgacatta aattttagat tggaatcacc cccttaaaga
    29641 aaataaaacc cttcgatatc aatttgaaaa gggtaaaaaa aagacaccta agaattttct
    29701 taagtgtctt ggcaaacaga ttttattatt cttgaactaa gaatgaagat acgccataag
    29761 aatttgaagt aattccttgt gctaacaagc catctgcata ttgatcaatc catgctgttg
    29821 cttcagcttc tgtatcaaat acttttgttc ctttttcaat attgtaagta gcgttagctt
    29881 catctgaagc agaataccaa actgtccatt tttcaactgg ttgtggtggt gttacatcgc
    29941 caccgtcatt accgccattg ttgtttccgt tgttgttatt tccattattg tttccttgat
    30001 tgtcttggtt tccattattt ccagtgtcgc cgccttgatt tccttgatta tcttggttgt
    30061 tatttccacc ttgattttga tcaggatttg gagttaccgt tccaccttga ttcccattgt
    30121 cagaaggtgt tgagggttga actggttgtt ctggagtatt tgctccgtta tctccagctg
    30181 gtgtagtagg tgcaacagat tggtcagctg agttagttgt attacttgtt gtatcttgag
    30241 aaactgcagg ttgccctact ggttgagttg gattaacttt gtctgtatct tgaataactg
    30301 tttgagctac tgtttcgcct gcgctatttt gaacagttgc aacattgcct tcaaatacta
    30361 atttgtttcc cgcatagatc aagtcaacat ttgcaatctt gttgatatca gccaattttt
    30421 gaacagtaat gtttgttgct tggctaatac cacttaaagt gtcgccccaa acgatcgtgt
    30481 actcattgcc tttgatatca gctttaattt gttctactgt acgtggtgtc cattcttctg
    30541 cgctcacttt ttcctgattg attgctaacg caattcctcc tactgttaca ccgacagcta
    30601 acactttcaa taatttcatg ttttattcct tctttctttt taatttttat tatattaata
    30661 ctataccagt aatggaatag tattgcaacc attttatatt agatttttta ctttttttca
    30721 tttataatat ttctgtagac aatcaaaacc acagaaaggt gggattaata tgaacatcac
    30781 taatatagaa gaactaaaag aatggattga aactaattta gttactaaac aagaagcaat
    30841 caagattact gaacaatctc ctaaaggatt tcagcagtct gttcgcacaa atgtaatcaa
    30901 accattttat gagagcaagg aaggaaaagg tccttcagta gttcgtctct atctaaaaag
    30961 cgatattgaa acctatgcta aacagttaaa agagaaaaag cagaaaccat aattacagtt
    31021 tctgcttttt ataaattgat attaatggag atatgctatg aaaaaatcac acatcgttac
    31081 attagcaaca attttcttac tttgcgttgc tgcatttttc ttttttacca actcgcccaa
    31141 aaacaataaa catatcacag cgaggacagc aaccacagaa agtacaataa aaacagaaac
    31201 tgtctatcct tctttgaatg aactaaaaca atcgcaaggt tcagatatag aaattctagg
    31261 gaagactcaa attactactg ttttatttca aaataagaac atggctattc taactacaga
    31321 gaacaacaaa gaaatacagc ttcccttaat tgtgactgat aagaaaaaca acttgtttga
    31381 aataccaagc ctcacttact caaagaaaaa tctaaaagtt ggtgaagtct tcggtttagc
    31441 taaaaaagat aatcactatc tagcatacaa attataaata gggagacatt tgtctctcta
    31501 tttttgcacc tcatttaaaa aattttacta tataatattg tgattattgt atcaaaatca
    31561 taaaatgata ttttgagatc ataatcgtta acttttgttt aattgaaata ctgtaataag
    31621 ctgacattct tttgtttttc tatacgctat gtcgatttga aagtttcttt gtaagagtga
    31681 tttaaatact gcttgccagc aagtatcata ttgtgacaaa tcatatccat atttatctaa
    31741 ccatttaata aaagtatcgt aagtaatgct acgaccaata tttttcttag catattgcat
    31801 aaccaatttt ttaaattcgg gtttattaga atataatact ttcttttttt cttcgatcat
    31861 tttattctcc ttaataaatt aaaatgttgt tttgtttatc cgttactgca attttgatat
    31921 ttaagttacc agactctcta tcttgtttta tttgcactga ctgatctttg atccatttga
    31981 tataattatc caagtttgct acttggttct tccttttcat atcacttaga aattgaaaaa
    32041 gctctttttc attttttaac cattccatac caacacctca taaaaattaa tttatttttc
    32101 ttctagagca attttttgca gttttagaag agtaacccac gctttctcta ctttttcgtt
    32161 attcctttta tcaatataaa aaagtttgct agttacactt tctggatatt ttaaaagtgg
    32221 atataaatcc caagtcagat attgaactct tcttaaagat acttcatgag attctttagg
    32281 attataattt ctatgagtgg ctaaatcata ttcaaaatac acttcgtcaa ttgtgttatc
    32341 gaagtaactt tgttcgcatt gaactctcat acctccccat attcccaaag aaaaaaatac
    32401 tagagctata cccacattta ccttaaaaat acttgagaac tcgtttttat tacttttacc
    32461 aaagcgaata cgattcaaat ttctcagctc ctttatatac ttgttaagga attaaagaag
    32521 taaactatta cttatgaatt gccaaaactc tttcttatca catattcatt attaatcaat
    32581 tcagaaagtg tatcacaagg ataaatacgt tttttacttc ttttttggtg ctcttttact
    32641 agccaatcca atttttctat agtttcatca gaaaatgcat acttttttct ttttttctca
    32701 gtactcatag gtgtaccctc cagttaaatt aggtgtcccc ttatttttta gatacttgat
    32761 tcttttgttt attagttttc tcataaatgt tacagttttt ttacgcaggg tttctagcga
    32821 tagcaacccc aaactctcga aaagtgtttt tgctccctag aaaaacgagc ggacggtttt
    32881 aatcgtcatc ttatgttttt cgtgatagct gatcatcgca aataaccttt tctgtgtgtg
    32941 ccctacccat caataacgta ccatctgata acacagccgt acttttttag gtggtttatc
    33001 cttcctaagc ccctattaga cacgctgatt aacttagaaa gtcctgtaat ttttcagttg
    33061 tcaaggaaca cccaaaacat caaagtaagt ttacgtgtat cgcttagtgt catgtgactc
    33121 cttgccagca aacgttaaat aaatatttag cgctgtttcg tgtccgtgtg cttacttgtc
    33181 cgttttttcg atagcaataa ctccctgcta ttaggttgtt tgttagaaaa aactaacaaa
    33241 caaaaaaatc aagtatcatt agtcaaaaca gtagctaggc actgattcgt ttatattttt
    33301 tgcgtgccgc actctttctt ttttcccatt cctctttttg catttctcta tatcgaactt
    33361 tcgactctct taagagcttc tgacgtgctt tcatttttga aaacacatga tttgaaacaa
    33421 ttaaaggcaa ctccgaacag cctctacaag caacgatctt gccgtcaaaa tagatagctt
    33481 ttatttgatc aaaaaagcaa gttaattcct gttcacttga taatttttta gcgtataaaa
    33541 taatttcttt acctgaaaaa tcagtaattt tacgcttgat cgttcgatta gaaaatttta
    33601 tgtgttgtcg attaaacaat acaaattcac aagtaatttg ttttaaattt ttaggtttta
    33661 attccatatt tttctccgcc cttctgcttc agttgctgat cattgggaag ccaagcacga
    33721 caaaaagagg gcaatcacac atgaagtgta attgccctct taagtcacaa atcagctgca
    33781 aacagtataa acaaatatat ctagaaaatt gcacactaag tctactcaaa aaagtagcca
    33841 gagaaataca aattttcttt taaagaattt aggtagcaca aataaactag ttaaattctt
    33901 aagaaatata tgttagaata tgcctgttgc agttaaatct gcaacaaaag tggcagttta
    33961 cctcttcagc gtggtacata cgttgaattg aggacggcta gcgattcatg acctgaagta
    34021 agagttggcg ctcttaccca gcgagatcac tagtcggtct gctctttttt gttggttgat
    34081 ttattgcttg gcttctcaat gatcagcagt ctggattgct aatcaataat ttttttaatt
    34141 tacgtttcta caaatttacg gtagcacatc ttcaaattca ttacaagctt ttttattttt
    34201 cacttgacaa atttgtgtaa gtactaatta tcgtaaaaca acataaaaca attatatttt
    34261 gatatacacc acctccactc acatagttgt gaagcgcttc tggaattatt ttaacattta
    34321 aaatcattga taacaaagct tacattacgc acttttcacg ttgtttatta gaaatactac
    34381 cgttatcgaa taaagaacac tcatgaaagt tttaatgaaa atttagaaac taaataacat
    34441 gatttattct aattttttct ttgttaaatg atcatgcaat atatatcaca ataaaattat
    34501 accactcatc gttcatattg caaacatatg ttcgatattt ttctaatcta accaattttc
    34561 aagatttaca gcaggtaaag agttagtttc tgcgtttttt tcatgtgcca atttagcagg
    34621 aatagctttc tcagcaaacc agttttttac gtaaataaaa attaggtttt gtaggttatc
    34681 aactttctct gttttctgtt tttgatatgc cttcagtaaa gtcatgtaca atcctttatc
    34741 tgcgtcaact ccataatact caatttcttc gtaaacaata attgtatttt ttgattcttg
    34801 ggctttatgc tttgcattgt gtatagtttt gactgtttgt tgtgcttcac tgtaactatt
    34861 tgaaaaagtt ttaatcaatt tcaaaacatt agttgggata aaggtgtcaa tgctggaatc
    34921 agtcattata tcaacaaaat tttctagcaa taattgatct tgtagctgat ctttgtcagt
    34981 gtctatattg tgtctattag tgtctaaaga attattagaa tataatatat tgtctgcgat
    35041 tttcgcattt acaggagaac tattggtatc actggctttt tctgtatttg cgattttcgc
    35101 atttacagga ataaccttga tatcatcgtc tttttctgta tttgcgattt tcgcatttac
    35161 aggatttggg attagtcgtt cttcttcgtt tattttaaat acgtcctctg aagttacttc
    35221 aggtttacct agatacaaaa ggtttggcgt agtttcaatt tttccgtttg cttttttgat
    35281 acgtcctttc ttttgcttca gcagtcctgc tgcctctaat tcttttttta ttttagaaat
    35341 tttaccttct cgacagttta gtaatctcat taactcttca atggtgtaaa taaaataaat
    35401 attatcctca gaatctaccc aattgttttg tactgagtaa tcaaaacgtg ctttcaaaag
    35461 catataggtt attttcgcat catttgataa atttaaatat ttctcgtttg taaaaaatac
    35521 tttaggcatt tgatagtatt tttcacgata aacttctttt gacttgtagt atttaaattc
    35581 cgacataata ttactccttt tattttcatg tagtggagta agtaattaaa atatgctata
    35641 atataataaa ttaaatcttt taattactta cacaaagact attgcaggac gccaatccaa
    35701 tcagcaatgg tcttttctgt atttacattt agtatacaga ataaaaccat taatctcaat
    35761 atttttataa aaacatttta atcagattag ctttgtataa aattataaat ttataaagtt
    35821 ataaaaatat aagttcataa aaaagattta ttttaagaaa taatgaaacg ctttaaatcc
    35881 atctatataa gcatttataa atacgtgttt ttatactttt ataaaattat aaaatattct
    35941 tgtttacatt ttatttcgat agcagtataa tatttttata aaattataaa attataaaca
    36001 ggaggtatta acaaatgaca aaagtattaa ccacaggaaa ttttaaaggt ggtgttggta
    36061 aaacaactaa tgctgtgtta acagcttata cattatctaa caagggttac aaagtcttag
    36121 ttgttgatct tgatccgcaa gctaatgcta cagagctatt atttgcgact atgaccaatg
    36181 tatacaaaat aaagcctgaa tttaaagaaa cattatttgt ttctattcaa aataataaaa
    36241 tttctaattc actaatctca gtgaaagaaa atttagatct attaccatca tttactgatt
    36301 tagaaaagta tattgattat ttagcggaat tatatgacga tgattattct aaagatacgc
    36361 atttctcaaa tttattaaat gaaattaaag agaactatga ttttattatc attgacgtac
    36421 caccacaatt aaataaattt actaattctg ctttagtcgc aagtgactat gttatcgtaa
    36481 ttctgcaaac acaggaacgc tcgttaaaag gagctgaaaa atatatagaa catttaattc
    36541 aattaaacaa tgattatgga acagaaatag atattttagg cttgctaccg gtactagttc
    36601 aaaacggtaa tgatttagac ctcgatatta ttgaagacgc agagtcatta tttggaaaat
    36661 cgaatatttt caatacaaaa atcaaaacaa tgcagagatt gaaaaggtat gatagaactg
    36721 gcataaccga taacccaaaa gacattcatg ataaaagagt ccaacaagtt tatcaaagtg
    36781 ttagtgacga agtaatacaa cgtattaatc ttttaggata aggagctttt aaaaatgact
    36841 aatgaaaaaa atgaatcaat tggtttaaat gccataaaaa gaaaaaataa aacacaacga
    36901 cctgaaaata ttgttccaga aaattctgaa gccttgaata atacaactga ttcaggcaaa
    36961 attctagaac aatccaaaaa acgactcaca actaaagatt tacctaaatc tataagaatg
    37021 ccactagata ctcacactgc aatcagcacg attgcgacaa ttgaagataa aaaaatatat
    37081 gaaacgttaa ataaaattgt agaattttat ataaacaaca tgaatccagc aaataaaaaa
    37141 attgtaaaaa at
//
LOCUS       DANKKG010000012        36947 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000012 DANKKG010000000
VERSION     DANKKG010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.012"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            44..1411
                     /gene="nhaC"
                     /locus_tag="PW004_000523"
     CDS             44..1411
                     /gene="nhaC"
                     /locus_tag="PW004_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDL2725519.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(1692..2711)
                     /locus_tag="PW004_000524"
     CDS             complement(1692..2711)
                     /locus_tag="PW004_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2725520.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(2733..3740)
                     /locus_tag="PW004_000525"
     CDS             complement(2733..3740)
                     /locus_tag="PW004_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HDL2725521.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            complement(3733..4398)
                     /gene="pgmB"
                     /locus_tag="PW004_000526"
     CDS             complement(3733..4398)
                     /gene="pgmB"
                     /locus_tag="PW004_000526"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDL2725522.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            complement(4391..6685)
                     /locus_tag="PW004_000527"
     CDS             complement(4391..6685)
                     /locus_tag="PW004_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HDL2725523.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            6945..9110
                     /locus_tag="PW004_000528"
     CDS             6945..9110
                     /locus_tag="PW004_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2725524.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            9414..10247
                     /locus_tag="PW004_000529"
     CDS             9414..10247
                     /locus_tag="PW004_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HDL2725525.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(10349..12049)
                     /locus_tag="PW004_000530"
     CDS             complement(10349..12049)
                     /locus_tag="PW004_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725526.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            12556..13716
                     /locus_tag="PW004_000531"
     CDS             12556..13716
                     /locus_tag="PW004_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL2725527.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            13732..14175
                     /locus_tag="PW004_000532"
     CDS             13732..14175
                     /locus_tag="PW004_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2725528.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(14658..15188)
                     /locus_tag="PW004_000533"
     CDS             complement(14658..15188)
                     /locus_tag="PW004_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HDL2725529.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            15293..15844
                     /locus_tag="PW004_000534"
     CDS             15293..15844
                     /locus_tag="PW004_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDL2725530.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(15997..16470)
                     /locus_tag="PW004_000535"
     CDS             complement(15997..16470)
                     /locus_tag="PW004_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL2725531.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            16608..18080
                     /locus_tag="PW004_000536"
     CDS             16608..18080
                     /locus_tag="PW004_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDL2725532.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            18077..18787
                     /locus_tag="PW004_000537"
     CDS             18077..18787
                     /locus_tag="PW004_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HDL2725533.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            19139..19423
                     /locus_tag="PW004_000538"
     CDS             19139..19423
                     /locus_tag="PW004_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HDL2725534.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(19740..20123)
                     /locus_tag="PW004_000539"
     CDS             complement(19740..20123)
                     /locus_tag="PW004_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HDL2725535.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIK"
     gene            complement(20125..20709)
                     /locus_tag="PW004_000540"
     CDS             complement(20125..20709)
                     /locus_tag="PW004_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HDL2725536.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(20756..22231)
                     /locus_tag="PW004_000541"
     CDS             complement(20756..22231)
                     /locus_tag="PW004_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HDL2725537.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            22412..22723
                     /locus_tag="PW004_000542"
     CDS             22412..22723
                     /locus_tag="PW004_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725538.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(22838..25174)
                     /locus_tag="PW004_000543"
     CDS             complement(22838..25174)
                     /locus_tag="PW004_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2725539.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            25316..25984
                     /locus_tag="PW004_000544"
     CDS             25316..25984
                     /locus_tag="PW004_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2725540.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            25987..27030
                     /locus_tag="PW004_000545"
     CDS             25987..27030
                     /locus_tag="PW004_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL2725541.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(27267..28148)
                     /locus_tag="PW004_000546"
     CDS             complement(27267..28148)
                     /locus_tag="PW004_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL2725542.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            28307..29035
                     /locus_tag="PW004_000547"
     CDS             28307..29035
                     /locus_tag="PW004_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HDL2725543.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            29191..29508
                     /locus_tag="PW004_000548"
     CDS             29191..29508
                     /locus_tag="PW004_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDL2725544.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            29501..30109
                     /locus_tag="PW004_000549"
     CDS             29501..30109
                     /locus_tag="PW004_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HDL2725545.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            30164..30955
                     /locus_tag="PW004_000550"
     CDS             30164..30955
                     /locus_tag="PW004_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HDL2725546.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            31093..31581
                     /locus_tag="PW004_000551"
     CDS             31093..31581
                     /locus_tag="PW004_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HDL2725547.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            31632..32504
                     /locus_tag="PW004_000552"
     CDS             31632..32504
                     /locus_tag="PW004_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HDL2725548.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            32680..33042
                     /locus_tag="PW004_000553"
     CDS             32680..33042
                     /locus_tag="PW004_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDL2725549.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            33051..33803
                     /locus_tag="PW004_000554"
     CDS             33051..33803
                     /locus_tag="PW004_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2725550.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            33817..35619
                     /locus_tag="PW004_000555"
     CDS             33817..35619
                     /locus_tag="PW004_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2725551.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            35896..36936
                     /locus_tag="PW004_000556"
     CDS             35896..36936
                     /locus_tag="PW004_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HDL2725552.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
BASE COUNT        11456 a         6547 c         7632 g        11312 t
ORIGIN      
        1 tttttcatgg cagggccttt tcatttgaaa taggagatcg acgatgaata aaaatatttc
       61 tttaaaagaa agtatcgctg tattgatcgt gttgttggca ctcctaggag tactgatcat
      121 cggattccag ctctcgccac atatcccgat ccttgcagct ttcatgtgtt tgttgtttta
      181 tggcagattt aaaaagttca catgggatga gatccatgac gggatcatca aaggaatcac
      241 tccagggatc attccaatcc tgatttttct tttgattggt gtcttagttg cttcatggat
      301 tttatctgga accatcccta cgattatggt ttatggattt aaacttgttt ctgtgcgttt
      361 ctttttaccg acagcatttc ttgtttgtgc aattgtcgga gtgactgtcg gcagttcatt
      421 tacaacgatt tcgacgattg gaattgcttt tttggggatc ggtcatttac ttggattcaa
      481 tgatgcaatg actacaggcg cagtcgtttc cggtgccttt ttagggaata actgctcgcc
      541 tttatcagat acgaccaact tggcagcggg tattggagga gttaatctat tcgaacatat
      601 cgctggtatg cgttatacgg atttcccagc attcgtcctt tcgttgattt tttatacgat
      661 attaggacat aacagtcatt cagccgatct agctagtatc aatgaaatga tccaaaacat
      721 gaaagcgagc ttttggatct ctccttggac attgattcca ctagttattt tatttggggg
      781 ggcaatcaaa aaaattccgg ctatccctac attactagcg ggatcgacag ctgctctcgt
      841 tctttctttt atccatgaga aaagtttgac ggtcggaaaa gcagcagaca tcttgatgaa
      901 tggttatgtc gcaaatacac ctgatccaaa gctagatgaa ctgctttctc gaggcgggat
      961 catgagtatg ttaggttctg ctagtttgat tttacttgca ttggcattag gtgggttact
     1021 gatcaaatat ctgatcgttg ataccatcgt tcaagaatta aaagaaaaga tggaccgacc
     1081 ttctcgttta gtcgggttta ctgccttgag ctgtatcgga atcaatctga tcgttggaga
     1141 acagtatctg tccatcattc ttccaggaga aacattcaag cgttcttttg atgacgttgg
     1201 cttatcaaaa acctatttga cacgaacact agcggattca ggtgcgaccg tcaattctct
     1261 cgtaccgtgg ggagtcagcg gcacattcat tatgggaacg atgaaagtta gcgcgttgca
     1321 ttacttgcca ttcgcctttt tctcgattct tgcacctgtc tttacaatat taggcggttt
     1381 tttattgaat cgtcgacgtg taaaaaattg aaggaaggaa aaaagaggct gggacagaag
     1441 cgtttaactc caagaaataa gaagaaactc aagaaaattg ctttttaaat ttttgtgaat
     1501 ttcagcttat ttccgaagga gttgcttctg cttccgccgt ttatacgtgt ttagagcgtg
     1561 agacaaaagt gttctttact tttgtcccac gctctttttt acagataaac aatgtgttag
     1621 aagtagtatc ttcttttaca taaaaagctg accataggat aaatggtcag cgtatttagt
     1681 gagtcagcta tttagtatgg gttttctaaa ggaacgactg tctctctttt gatcaagcga
     1741 tgagggatga ccaaacgtac gccactgctc aattcttcat ggatcaattc gcgcagcttt
     1801 tgcatagcga cacgtcctaa ttcggagata tcgatatcga tacttgtaag gtaagggtgt
     1861 gttaatgttg agaagatcga gttgttgaag ctaatgacgg agagtgtctc tggaatgaca
     1921 tagccataca tttgcgccaa ctgcatcgtg cgaagggcga agatatcgtc aatgaccacc
     1981 aatgctgtgg cttcgttttc tttcaacgtt ttctcaaagg acagataatc ctccggatgc
     2041 tctagtacta cactggataa cgcaggaaga ccagccatca tcattgcttt ttgataaccg
     2101 aaatatcgtt cgaaatacaa actttcatga gtcgtgtttg tcacaaacag gatattctta
     2161 tgtccattct ggatcaaaaa ttctgttgct tgtttaccta gaagctggtt atcattatct
     2221 acataaacga tttcgttctc atgatgataa ggctgtccga tcaaagtgaa agggatgtgg
     2281 ttttggtata aataatcaat gacagtatct tgactatctg aatacactag aataaaacca
     2341 tctacttgtt tttgtaaatg catacgctgg acgttctcta acagcaattc aaaattttta
     2401 gcagtagcga ttgctgtagt catatcataa ttccgcgctt cttcgttgat tgcttccatg
     2461 atctcaagat aaaatgggtt accgatccgc tcttttgaat caagaggggg taagattacg
     2521 ccgatggcac tggaaatgcg ttttcctaga ttgcgggcag tcatgttagg aacataacct
     2581 agttcatcca taatttttcg gactttcttc tttgttgtct cggaaatact aggatgatcg
     2641 ttgatgacac gagagacggt cgaggtggca acaccggctt ttttcgccac atcttttacg
     2701 gtaatagcca tgataaaaac acctttctct tgttattgtg cagagaaccg ataaatcgtc
     2761 tgactgatcc gttcttgacc aggatggagg ttgatcgatc caaatttggg gaaatgaacg
     2821 atatcaggga tttcttgcgg ttcgattgct aatccgtagt ttgaatgcat ctgttttcca
     2881 ttgacagaga aatcagcttc gaatccagtc gtagaaaata ataccatact tttattagaa
     2941 gttgctatag tcatttggcg accagaatgc ttctcgaata attgtaagga agttttggaa
     3001 agcctaggat gatgcaaaag aaatacatca tctagtccat ctggataaca tgcaaggatc
     3061 tcttgcagtg ttggggattt tctaaagtcg attggcagtt tttctttttt tgtccaattt
     3121 ccagtaggca gtttttcttg atctaattca agcacaccat ctaaaaagac cgttaactga
     3181 tgttcataga tgtctcttgt tccatttcct gaaaggttga agtaggcatg gttggtcgga
     3241 ttgcagatgg tttcaacaag gctactagca gaggttgtca tctctaattc gttttttgtt
     3301 aaacggtaag tgatcgtagc agtgattggt cctggatagg tagaaaattc atcttttaag
     3361 taaaagacca cacctattga tgatggagtt ttgaaaactt ctacttccca aaattggaaa
     3421 gaccagcctt tcgtgccacc gtggatatgg tgtgatccgc agtttttctc caatctatgc
     3481 tcaccccaag aagcatcccg gatccttccg gctacaggtc cgacagttgc tccaaaaaag
     3541 cttttgtctg ttagaacatc ttcgaaacga tcgtaagaca ataaaacatt ttctgatttt
     3601 ccttctttgt ctggtgcgaa gatttggtgc aggcgggcac cgtaattgag aagaacgact
     3661 tctaagtagt cgttcttcaa tgtgattttc tttatttcag tctctggaac tggtacgacg
     3721 gagatctctg gattatccat ggtcatgcca cttttctttc aagtaatcga tcgtaagaaa
     3781 agtcgtatca gggacaagcg ggatctcctc tccaaggtct tcagcacgtc ccacgccaat
     3841 tggttgagcg ccacttgaga ggatggcttg gataccggct tgcgcgtctt cgatcccgat
     3901 acattcttct gcttttagtc cgacagcttt tgctgcaagg aggaagatat ctggcgcagg
     3961 ttttccgttt gttgcttctg ccgggtcggc aatagcatca aagtaaggtg taagacccat
     4021 tttttcaagt aggaatggcc cgtttttact tgcagaagca agagcaatct tgattttttc
     4081 ttcttttaaa gaatccagta aaggcacgac cccggggaag acatcttttg gttcgatcgt
     4141 ctggatcatc tctaaataat aatcgttttt tcttttcgct aattctgcaa attcttcctt
     4201 tgaaaaagag tgttcttttc cgccataagc taaaagcaaa gagagtgagt cttctcggct
     4261 gacgcctttt agctgttcgt tgaatgtgtg gtcgatcgta atgccgattt cctgaccaag
     4321 ctttcgccaa gcacgataat ggaattcagc agtatcggta atgacaccgt ctaaatcaaa
     4381 caatacccct ttaaacattt gcttcctcct ttcttagcgt tatttctaga cgatccgcta
     4441 aagaataaga ctgttcatag acgtaaattt ctagaggaga accttctaat aactcgaaag
     4501 taacttgttc atctgtgact gagatgaaca ataaacgtcc gcgataattg atatggaaag
     4561 aataagcttt ccaagttttt ggcagaaatg gtgcgaatct caactgatca tgggcagttt
     4621 tcatttgagc aaatccttgg acaatcgaca accagctacc agtcatggaa gtgatgtgca
     4681 agccgtcttc tgtgtcgtta ttgtaattat ccaaatccaa gcgagctgtt cgctggtaca
     4741 tttctacagc tttgctttcc attcctaatt ctgctgctaa aatcgaatgg atacttggcg
     4801 aaagggaaga ctcatggaca gtcattggtt catagaaatc aaagtttttt tgcttttctt
     4861 cttttgtaaa agtatcattg aagaaataaa tcccctgtag tacatctgcc tgcttgatga
     4921 agcaagatct aaggattttg tcccaagacc aatgctgatt cagaggaaga tcgcttgtgg
     4981 gaatatcgga tacaggcatg agatctttat ctaagaatgt gtcatgctgg acaaagattc
     5041 ctaattcttc gtcaactggg aagtacatgt tctctgcgat atctttccat ttggctagtt
     5101 cttcttcgct gatagtgata gcagcttctt tttgataagt taaataattt tctcgcgtgt
     5161 aacgcaagac ccaagctgcg atcgtatttg tataccaatt gttattgata ttgttctcat
     5221 attcgtttgg gccggtcaca ccgtggatca tgtatttatt tgctcgtttt gaaaaatgga
     5281 cacggtctgc ccagaagcgt gcgatttctg ctaaaacttc cagtccttca tttttcaaat
     5341 attccgtatc gccggtgtaa ttcgtataat tgtagattgc ataggcaatc gcgccatttc
     5401 ggtgaatctc ctcgaatgtg atttcccact cgttatggca ttcgacacca gtgaaggtga
     5461 ccataggata aagcgcgcct tttaatcctt gttgctgagc gttgtggatt gcttgcggca
     5521 gttgattatg ccgatatttc agcagattct tagttacttc aggtttggct aaagcaagat
     5581 aaagagggac agcatacgct tctgtatccc agtaagtggc tccgccgtat ttttctcctg
     5641 tgaatccttt ggggccgata ttcaatcgtt catcttcgcc gtagtaagta gagaatagtt
     5701 ggaacaaatt gaatcgaata ccttgttgtg cttcttcgtc tccttcgatg accacatcgg
     5761 caagcttcca tcgttctgcc caagccgctg tttgctcttc ttttgcttgg atataattgt
     5821 ttgtctggtt cgttaattct gagacagctt tttctgcttg ctgcgcttct ggaatatctc
     5881 tactagtaag aacgataatt tctttttcca ctgaccatgt ctcaccttga gataattctg
     5941 cagacaagca accatgtaat gctaaagtat cttcgtgata gtcagggatg actgattttc
     6001 cattaacgaa atggcgcatt gctgcagtaa cagtaaattg ttcaatccca aagttgtttg
     6061 gaatagtttt agtcgtcaaa tagccgattt cttctatagt tccacgatca atctcattcc
     6121 aaaaatgttc atcatagttg ctgtcttcgt tttgaacatt tccatccaat tttgatacga
     6181 gtttgactgt tccgctacct tcaagcattt ctatagttaa atagatataa gctgattctt
     6241 ttttgacaat gctcagaaaa cgttcgaagg aaatgcgtac tttattagta gaagtagtga
     6301 ttgtgaatgt acgagataat acaccatttt tcatatctaa ttcccagtaa aagtcttctg
     6361 gttccaaaga agccaagtcg atcagctgat cgttgatcga aatatctatc gcgataaagt
     6421 tcaaggcgtt gatcactttt ccaaagtatt ctggataacc atttttccac cagccgacac
     6481 gtgtcttatc gggataccag acacccgcca aataagtacc ttgatggtgg tcccctgagt
     6541 aagtttcttc aaagttcccg cgcatcccca tataaccgtt tccaatactt gttaaagatt
     6601 cttgcagtcg aagatttttt ttatctaatt gtgtcgtgct gattttccat ggatcaatag
     6661 caaatagtcg tttgatctgt ttcatttttc tcctcctaat gagctgtttt atacgttcat
     6721 aataaacgca accgattgca caagtcaata gacaaaagaa aatatttcat agaaaaaagc
     6781 ttaaaaaaat aaatttttag gagaaaatta tagaagggaa cacacgtatt ataggtttta
     6841 tagaatggta tagacagaaa aataaaaaat aaagcattta caaaaaagaa aacgcacgct
     6901 ataatacgtt taacgaaacc aattgcgtaa aggagctaaa cggaatgaaa aagatattta
     6961 gttttgagtt ttggcaaaaa tttggaaagg ctttaatggt tgtggtagca gttatgccgg
     7021 cagctgggtt gatgatcagt atagggaaat caattccctt gatcaatcct gattggacgc
     7081 cgcttgtcac aacgggcgga gtaattgaaa atattggttg ggcaattatt ggtaatcttc
     7141 atattttatt tgctttagca ataggaggaa gttgggcaaa agaaagagca ggcggagcat
     7201 ttgcagctgg gatctcattt attttaatca atcggatcac tggggctatc tttggtgtca
     7261 caagtgacat gcttgcgaat gaagatgcat ttacccacac tctgttcggt acgaagatca
     7321 tggttaaagg attttttaca agtgtattgg aagcaccagc tttgaatatg ggagtattcg
     7381 tagggatcat cgctggattt gtcggggcaa tggcttataa taagtactat aattatagaa
     7441 aattgccgga tgctttgtca tttttcaatg gaaagcgctt tgttccgttt gttgttatct
     7501 tatggtcaac aatcgtggca ttgattcttg ctgtggtgtg gccaaacgta caggctggaa
     7561 tcaataattt tggtttatgg attgcagaat cacaagaaag cgcaccgatt ctagcaccgt
     7621 ttttatttgg aacattagaa cgtttactat tgccatttgg attgcatcat atgctgacga
     7681 ttcctatcaa ctatacacag cttggcggat cgtatgagat tttatcaggc gcacaggcgg
     7741 ggacacaagt atttgggcaa gatccgttat ggttggcttg ggccactgat ttagtgaatc
     7801 ttaaaggtgc aggggatatg tcgcaatatg agtttgtgtt gcataactgg acgccggctc
     7861 gtttcaaagt aggacaaatg attggttctt caggtatctt gatgggaatg gcttttgcga
     7921 tgtatcgtaa tgtcgatcct gataagaaag caagatataa atcaatgtat ttttcagcag
     7981 cgttggctgt ctttctaaca ggtgtaacag aaccattgga gttcatgttt atgtttgctg
     8041 ctgtgccatt atatgtcatt tattcagtga tccaaggggc tgcatttgcg atggcagata
     8101 tcttgccatt gcgggtccat tcatttggga atatcgaatt gctgactcgg acaccgttag
     8161 ctatcaaagc gggtctaggt ggagatctta tcaacttcgt gttgtgtgtg atcgcttttg
     8221 gtgttgttac gtatttctta gcaaatttct tgatcaaaaa attcaacttt gctacaccgg
     8281 gacgtaacgg aaattacgac aatgacagtg aagaaaccgt tgtatctaac agcggaacag
     8341 gaacagcaga tcagcaagtg gtccagatca ttcatttgct gggaggaaaa gaaaatatca
     8401 aagatgtcga tgcttgtatg actcggttac gcgtaagtgt aaatgatcga gaaaaagtag
     8461 ggactgaaga agcctggaaa cgagcagggg cgatgggatt gatcgtcaaa gacaatggag
     8521 tccaagcagt ttatggacca aaagcagatg tcttgaaatc agatatcgaa gatctgctgc
     8581 aatcaggagc agagattcca gagccagaaa tggtttctac aggaaatgga caagcaggta
     8641 gtaagcaata cttaggtata gaacaagaac tagtttccgc agcttctgga gaagtgattc
     8701 ctttatctga agtaaaagat ccggtattct cacaaaaaat gatgggtgac ggctttgctg
     8761 ttattccaac atcaagagaa gttgttgcgc cgatcgctgg aaaagtgaca agtattttcc
     8821 ctagcaaaca tgcaattggg ctggaaacga aagatggtat cgaagttttg atccatatgg
     8881 gaatcgatac agtccaaatg aatcagccag cttttgagat tgctgtcaaa gaaggccaag
     8941 aagtaggaac aggaacgaaa ttagctgaga tggatcttga tgtgatccaa aacgaagaaa
     9001 aagatccaac gatcatgatc gtatttacaa acgataaagt agaagaagta gtgatcaagc
     9061 aattaggtac agtgacagct ggtaaagtga tcggctccat taaaatgtaa ggagaggttg
     9121 tgaaaaagct gtcctgcatc aagtcataag aacagccaaa caaaaatagc tcctcctatt
     9181 tttcgtttgg ttgggcttat taccgagaag cagcagtttg aatactgttt agtggagagg
     9241 tcgtgaaaaa gctgccctgc accaagtgat aaaaacagcc aaacaaaaaa tagctcctgg
     9301 tccattgagc ttattactga ggagttagca ggagaacact atttgctgga gaggttgtga
     9361 caaaaatttt gtcacaaccc ttttgtttcg aaatgaagac aaggagaaaa ccaatgaaat
     9421 tcatgacact caacacgcat agctggcttg agccagaacc agagaaaaaa ttgcaagaat
     9481 tagcagataa gatcctgtta gaagattatg aagtgattgc tttacaagaa atcaaccaat
     9541 tactggaatc tgaagaagta gaacctgcga agctaatgaa gttctgtccg gtaaagaatc
     9601 aagtacctat ccgtaaagat aattttgcct accgtttagt tcagctttta aaggaacacg
     9661 gaaaagagta tttttggagt tgggaaatga gccacatcgg ttatgataaa tatgaagaag
     9721 gaaatgcact gcttacgaaa aaaacattgg aaagtcaggt ccttaccgtc tcccaatttc
     9781 aaaagaaaga agattatcag acacgcaaga tcttgattgg gaaaacaaag atagatgatc
     9841 aggacatttt tgtctcgagc tgccactttt cttggtggac agataaaaaa agtggctttt
     9901 attttgagtg gaaaaacttg gaaaactact ttttggaaac tcgcgtacca ctttttttcc
     9961 taggtgattt caataatcca gtagactctc aaggttatta tactgtaaga gaaagttgcc
    10021 ttcttcttca agattcgtat gttgtcgcga atgaaaaagg aaaagcagct actgtggaaa
    10081 agaaaataga tggttgggaa caaaatacag aaaaattgcg tatagatttt attttcgtcc
    10141 ctgaaggaat gcaggttaaa aaatatcaac ggatttttga tgggatcgat agtccaatca
    10201 tcagtgatca ctacggggtg gaaatcgaat tagatgtgaa cgaataaaaa atataacata
    10261 taacataaaa aaactgatta aaataaaact ctccaaacga ttataccgtt tggagagttt
    10321 tttgatcgga tgtatttatc ccttttgcct aagcacgtaa aagcttgtat ctatgataaa
    10381 taaataaaca tagcaaggta aaccagccaa tcaaagtaag agaaagaatg aaatagaaat
    10441 agattggcgt ttgataatga agcgtgagct cgttgattcc tttttgctgc ttcacgattg
    10501 gggtcccgat atctgttact tcataatcag tcaatgtttt tccattttgc tgtagcacag
    10561 tccgatcgta gacgataaca gggatatgaa cagttttgtc cccagtgttt ttccacttga
    10621 ttgtcaaaag accgcctgga tgtgttttta taaaagttgt ttcgttttct agtatttctt
    10681 cgttataccg gtcgtattta ttgtcttttg tctccttata gattggcaga tagtcaggtg
    10741 tggttttttg gaaaacgaac aagctggtag acatatcttt gctgaagaat gttttccttg
    10801 cttcttccgg cgtatcgaat agataagtat gtcgactgac aaatgtttca ttttcccact
    10861 tttccagatg ctggtataag ttctggatcg tctgtgctga aaaaataaga atgccaaata
    10921 tacttaaaag acggaaccac ttttgttcag accactggtt cagtgaaagg gcaaggtaaa
    10981 gtagaagcaa tagcgtaaat ggcacgaaaa atcgaaatgg aaactggatc aaactaataa
    11041 aaggcacatt tttttcgatc aatgtcgtcc atggaatcaa gctagtagaa agaaaaaaga
    11101 aaacaagact tgtgtacaac acgatttttt ggaataaggt gttcttttgt tgaaagaaaa
    11161 taaaaagcaa aaaacctgat aatatcaata taggaaaggc cttagggtaa tggagccaat
    11221 aactgccatc cagatctatc gccttttgac tcatttctct gttaacaaag ggaggaagaa
    11281 tcttattccc actgtaaaca atagctaatc ctgcccaaat atttgctgtc agaacaaaat
    11341 aaagcaagac cgacttaatc agttgaagca gtgcttttcc tttttcagtc tggttaaaaa
    11401 agtaataaag ataaaaaggg atatacatca aagctaacat gatcgaagac agtaaatgga
    11461 tctgtgtcat aagtcccact gagacagcga ctttgataac agggaacttt ttatgaagga
    11521 cgaaatcagc aatagggatc atacataaag gataaaaaca agtcccccag ctagtgaatc
    11581 cttggttgat agaccagtat tgaatagaat acgtactcat gaataaaacg gctactggta
    11641 cacttaccca atttcgaaca ttgacatatc tcagtaaaat cagcatagaa aatcctgaaa
    11701 taagataaag aatgaaattt gaaagaatct gataatgaaa ccagctacct gcaagcaaca
    11761 cgatcaatcc ttgtaaataa gcgaaaaatg ggccgtaaac ggcattcacg atcctgccac
    11821 tttgctggaa tccataaagg gaaggaaaat aatggaaatc cccatattta atttgttgcg
    11881 cggcatcata gaaccgattg taatggaatg gggcatcagc acccaaaatt agtttatgtg
    11941 tgaataaatg aggcatgaga aatgccaaag caatgaaaaa aattgctaaa aatgggccat
    12001 attttttcaa ttgtcgaact cctttgcaaa ctttagataa attcatcata tatcctatgc
    12061 gagaggtagg caagaaatta gaaaattcat gaaaatattt atgaatttta caagaaaaac
    12121 ataagtgaaa gttctaagga gagaggatgc gacataagtt ttgttgcatc tccttgtttt
    12181 gaaacagaat ttttcttaat actcgaaaca aagtagcttt ttcatgacct ctcggtaaac
    12241 ggtgttcaat cagctgcttc tcggtaataa gctcactagc ctagaaaata tgagaagcta
    12301 ttttctgact agcgctctta ttactcaaag cataacgcag ttttcacaac ctcttcctta
    12361 aacggtgttc aaaagcttgc atctgcggta ataagcccaa cccaacaaaa aatatgagta
    12421 gctatttttt tgggctgtac ttattacttg atgcaggaca gctttttcac aacctcatcg
    12481 gctttcattt tctatttttt tcctgaaccc atataataaa agaaagaaca aaaaaacttg
    12541 caagggagga cacatgtgaa gcagagtaag caaaaacagt ctcgtcgact tttgtttcgt
    12601 ttgtttttat taatatgtat cgtcgtaggg ttactgatcg ctttatatcc attttatgtg
    12661 gattctttaa atagttttat tgatcaaaaa cgtatcgaac aagtacaaaa acggaccgca
    12721 gctgaaattg aagtgcagcg aaagaaaatg gaggagaaaa accaacgatt aactgaccaa
    12781 ggattcaatc ctggtactga tcctttcaat gaaaagaaca gaaatgaaag tacaacatcc
    12841 agccaattag aggaatggct gatcggaagt atcaatatac ctaagatcca attaaatatt
    12901 tcactttatg atcggctcaa tggtaggatc cttgaaaatg gagcaggcgt cttgcaagga
    12961 acctctttcc cgcttggtgg aaattcgacg cattcagtga tctctgctca cagcggttta
    13021 cctaatcgac ggttatttac cgaattagat cgtttagaaa acggagacac gtttattttg
    13081 actgttttgg gggagaaatt ggcttatcaa gttaaaaata tacaagtggt actgccagat
    13141 gacacgtctg ttctaacgat ttcagaagga gaagacttag ttaccttgct tacatgtacg
    13201 ccgtatatga tcaacacgca tcgtttgcta gtgacaggac atcggattcc ttataacgaa
    13261 tcggtaaaaa aagaagaagg aaaaggaaat caggaaagaa ccatgcggca atggtttatt
    13321 cttgcaggaa cgataatagc tgtagtcata ctgttagtgt ttatcggacg actaatctat
    13381 cagtatagat taagtaaaaa agtgcttgat ttttcattta ttattagtga ttcgtcaggg
    13441 aaaccggtaa atggtagttc attcattttg aaacataaga aaaaaacact tactagaaat
    13501 ggtgtgccgt tttcagtaca aagcgaccat catgggaaag tcaagctgga tcaattgcct
    13561 ggtggaactt atcgaatcgt ttcagctgat gatcctaaag ttgctgtttc ttttggtatt
    13621 agaaaagtaa aacaagagaa gatatacttt tttgaggggc gaaaacttgt aaaagagctt
    13681 caaaaaaatg gtttttattt taaaatcaat gaataagtaa ggagagaaaa aatgatccgc
    13741 acaacaacaa aattgtctgc caaagaactg gaatctgttc tatcgatttg gctgcatgct
    13801 aacttggatt ctcatgaatt cattccatca acttattggg agaatcaagt agaatctgtt
    13861 aggaaagctt taccagatgc agtgatttac ttagcagaaa tagatcaaga aattattggt
    13921 tttgctgggg tgattgatca gtttatcgca ggaatctttg tgaaaaggga ataccggcaa
    13981 caaagaatcg gtacgcaact aatgaaaaaa ataaaagaaa ccaataaaac actgactttg
    14041 acagtctatc aaaaaaatca atcagctatt catttctatc tttcacaagg ttttacaatt
    14101 caaacacagc aaaaagatga agcagtaaat gaggaggaac ttgtgatgaa atggaatcaa
    14161 aacgctgatt tttgatgaac agaataacga aataatgctc ccagcctaat tttttgctgg
    14221 gagcattatt tcattttgaa gtcactaatt taactgtgtg aatatgcagt tgttacaatc
    14281 tttgataaac ggtgttcaat cagctgaccc tcggtattag ctcaaaaaac ggcaaaatat
    14341 gaggaactat ttttccattt gtttctctaa tactcaggtc aaaacgctga tttcacaacc
    14401 tcttgataaa cggtgttcaa acggctgctt ctcggtaata agcccactag cctagaaaat
    14461 atgaaaagct attttctaac tagcgttctt attactcaaa gcataacgct gatttcacaa
    14521 cctcttgata aacggtgttc aatcagctga ccctcggtat tagctcaaaa aacggcaaaa
    14581 catgaggaac tatttttccg tttctttctc taatactcag gtcagaacgc tgatttcaca
    14641 acctctcttt cacaacctta ttttactcgt ttataatttt catcgaagta ttttccgcta
    14701 cggatatcat ctggcattcc atcgtatggt tcttcgctaa taacatcttc attgtctata
    14761 cctgcatcac ccatataagt gatcgtagca aattcaggaa caagtaattt tggataagtt
    14821 tcatattttt cacgagctgc ttccatttct gcgatatgtt cgttttggaa agtaacagtg
    14881 atttctggat gttcttcaac ccatttaggg aaaaagatca ttccgtgaag tttcttttca
    14941 tttggcataa agtccaatgc cacgtctgtc cctgttggct ctgtccaagt gattttgaat
    15001 acgccgtccg tcaatttaac gatatcagct tcttggtctt ttacccaacg gcctttgacc
    15061 attccgccat ggatacggta atcaacagta tggtcatttt tggcatacca ttcatattcc
    15121 caaccattgt catacgtata gataaagtga gttccaataa aatcttccaa ttctttaaat
    15181 tctttcatgt ttaagcatct ccttataaat taaaacatgt aataaactac atgttttcga
    15241 ttactcattt atgatagaat ggatttaaag atttgtcaag gagggaaaac tgatggcaag
    15301 aaaaaataca cttcgatata ttttgttagg gttattaagt aagaagaaga tgacaggata
    15361 tgaattgaat caatcgttca aaaatgaaat aggagaattt tggcaagcaa aacatagtca
    15421 gatttatcct gaacttgcaa aaatggaaga gcaaggaatc attcagcatc aagtagagat
    15481 cacaggtgaa aaattggaaa agaaagttta tgaattaaca gaagaaggga aggaacaatt
    15541 ggcagagtgg attcatacac ccaccaatga actaccggtt aatcgagacg aatttgttct
    15601 gaaattgtat ttcgtcaaag acgtgaatga tccggcatta gtagaaatta ttcagcagca
    15661 gcgtgacctt catgaagaaa aacgacttca tctattagag cgaaaaaaaa cgatctttcc
    15721 tactgaaaaa gaaaaacatg aaaattacgg acactacctg attttagcgc atgcgatcaa
    15781 tcgggaaaca gaatatgcaa attggctaac agatgtatta gaagaaagta accgaaaaaa
    15841 ataaaaaaat atccgaacag tgatggagaa ttcctttcta tatgaaagaa acgcagatca
    15901 ccgttcggat attcttatgt tcaaacaggc cttttttcaa gtatttatgt agcatctact
    15961 tattaaaaag cttggtactt aggagattag tggcgtttag agttctttta acaactgttc
    16021 gtagtcagct ttttcatcga caggaaggag cttgtaagtt aattcgtctg tctcgttgtc
    16081 atgaatgatg tacatcttag cattctcttc tttgaagtta ccgatttgta aaatcgtata
    16141 gcgttcgtcg atcaattcga tatctttgat tgcttttgaa aaaactttac ggacatggtg
    16201 cattttcttt tggcggattt ctgccttttc tgcagctttt gacaaagtaa atccgtcatc
    16261 taaatatttt ttgatcagtt ctacttttat gactgtaggt aaacgatatt ttctaggaca
    16321 attcgcatca gcttcgatcg aacgaatgaa tcccttttgt tcccaatacc ttaattgacg
    16381 tggagacaca cctacgatat cacttaattc actgatgcca acaaccaatt gatcattttc
    16441 caacaaacgt ttaagggaag ccccaaccat ttttgtcact ccttttgtcc tcttgcttcc
    16501 cttaattata tccgtattaa cttatgtgtc aagtaggttg acaaatagcg aatttaggac
    16561 taaagtattc tagtagtttc agaaaaaatt cagaaagaag gagaaatatg agtaacaacc
    16621 aacccgtaga tatttacgga aaaccatata atcggacact tttagttgtt gtcttattaa
    16681 ttggtacatt ttgtaccgtt ttgaatcaga ccttattgac gacagctttt cctacattga
    16741 tgaaagcttt cgatatttca gcgtctgatg tgcaatggtt aacgactggt ttcttgctag
    16801 tcaacgggat catgatccca ataacagcat ggctgatcaa taaattcagt tcaagaaatt
    16861 tatatctttc tgccatgacg atctttttga tcggaacgat tacttgcttt acagcaccta
    16921 attttagtac attactaatc ggtcgtttga tccaagcttg cggtgtggga gtatcgatgc
    16981 cattattgca aaatatcatg ctatctattt tcccgccaga aaaaagagga tcagcaatgg
    17041 ggatgtcagg gatcgttatt ggacttgctc ctgctttagg ccctacatta tctggttata
    17101 tcattgataa ctaccattgg cgtgacttgt ttggtatggt actgccaatc gttgttcttg
    17161 tcttggtttt agcattcttc ctaatgaaaa gtgttattcc attgtcaaat cctagcattg
    17221 atattttatc cgctatttta tcaacgattg gattcggtag tttactttac ggattttcta
    17281 gtgtaggaga tgacggctgg ggaagcttga aagtaattgg tttcttagtt gttggtgtga
    17341 ttttcatcgc cttgttctca tggcgtcaat tacatttgga acatccattc ttggaacttc
    17401 gtgtattcaa atcatctaca ttcacgattg cagctgtttt agctggtgta acaaatatgg
    17461 cgatggtagg ggcagaaatg gtcgtaccat tgtatattca aaatattcgt ggagaatcag
    17521 cattccattc tggcttgatg ttgttgccgg gtgcattgat catgggggct atgatgcctg
    17581 tgacgggtgc gattttcgat aaatacggcg cgaaacgttt agccatcaca gggatgttca
    17641 ttttgacagc ggcaactgca ccatttgctt tcttgacaaa aacgactcca gttttatata
    17701 tcgtgatcct ttatgcgatc cgtatgtttg ggatctccat ggtcatgatg ccggtaacga
    17761 cttctggtat gaatgcattg ccaaataact tgatcagtca tgggacagcg gtaaataata
    17821 cattccgtca aatcgcaagc tcgattggta cagctatttt gatcagtgtg ttgacgaatg
    17881 tgacaaaagc aaatatccca agcaaatctg tattggataa tacgccatta gcttataaag
    17941 atcaggcaat caatgccaca ctgtcaggtt accatgccgc gtttatcgta gcgatcatct
    18001 ttgggattct tggctttatt attgcctttt tattgaagaa aaaagaacct gttctaatga
    18061 aagcggggga taatgcatga ttattggagt tttgattgtc agcttgcttg cttttgcttt
    18121 aacgaacgtg ttcgctaaaa aaacatggca aaccttttta tctttgattt tcggactaat
    18181 cttcgtcgct tcactaagtt tgatcgtcgc taatttgtcc aatcactttg gaatggaaaa
    18241 agtaacggaa acaaaaacag aaaaaattgt ttccagtgct gattcccaag gagcagatat
    18301 gcttttgtat aaagctttag ggaatggaaa agaaaaagtg tatctttacc gaacaaatga
    18361 aaaacaagaa aaaccaaaag caactggtac ggataacgaa acaaataaag tagaaaagac
    18421 agacggcgat gctgaaaaag tgacgaaaac tacttattgg gaatacaaaa acgacatgta
    18481 taaattctgg ttcaatattg ctgataacaa tcacgaatat gacagccgag tgaatacatt
    18541 caaaatccca gagacatggg tagaattaag tacggaccaa gcagccaagt tagctgaatt
    18601 agtgaagaaa cagcaaagta cgatggaaag tgaagcaaaa gcttatgttc aagacggtat
    18661 ggtaaaagca atgactgaaa atccaaaaat gagcaaagca gagcaagaac aacgtacgaa
    18721 agccttagct gctgaattcc aacaacaagc attcgcaaaa ttagtgaaag aagcaaaagg
    18781 cgaataatat tttgaacacc atgtaggagg ttgtgaaaaa gctgctctgc atcaagcagt
    18841 aagaacaatc agataaaaat agcactccat atttttcatc tgattgggct tagtgccgta
    18901 gatgcaagct tttgaacacc gtttaaggaa gaggttgtga gcctgccgtt taactctgag
    18961 tcacaaggag caatttcaaa aaacagcttc tcatgtttgt tgactattgt gacttgtgac
    19021 cgaggagttg gcaggagaac accgtttatt cgaaaaaagg aactgcgaca agacttttgt
    19081 cacagttcct tctttttttt gctatgctat actagatgta aaaaatagga gaaataaaat
    19141 gagggaaatt tattcttttg aatcaaaaat ctatgcttcc gaagtcggca agggcggggc
    19201 atatattatt tttccgtatg atataagaaa agaattcggt gctggaagag ttaaagtcca
    19261 agcaactttt gacaatgtgc cgtatgaagg cagtatcgtg aatatgggcg tgaaaaatcc
    19321 agatggttca gtctgctaca tccttggact acgaaaagat attcgtaaaa acatcggaaa
    19381 agaaatcggt gatattgtag cagttacagt gaagcaaaaa taaaagaggt tgtgacaaaa
    19441 gacgtgtcac aacctcttga taaacggtgt tcaaaagcca gtttctcggt agtaagtcaa
    19501 atttatgcaa aaatgtggaa acccatgttt acaaaatttt tcttactact cgaaacgaag
    19561 cggctttatc acaacctctt gataaacggc gttcaatcag ctgtccctcg gtattagctc
    19621 aaaaacggca aaatatgaga agctattttt ccattttttt ctctcatact caggtcataa
    19681 cgctgatttc acaacctctt ctttcataat ggtttttcac catctttata ataaactggt
    19741 tatttgatat ggaaatctat tttttccaac caatctaaaa tcttgtaacc taactcttct
    19801 gacatcggaa aagaggcgtt gatttgtgca ttggagatat ggtcaacatg gataccggaa
    19861 gtgatcgtac aacttcccgg aacggcagtt ttgatgatca cagctatttt ttcggctaag
    19921 acatcgtctt tatggaaccg accatcgtgg ctagggaaac gtacagtttg catctctgta
    19981 tcacgagaaa atgtagtgac tgttccgata tgtggtgtat ctccgcctgt taaagtgatc
    20041 aataagtctt taccgattac tttaacagca gcttggatcg tgaaacctag tttggttact
    20101 tcaactatcg tttctttttc catattaatc accctgccaa cgttttgaat gctcatttac
    20161 aatttcaaat tgatccaaaa gtttcattgt atggtgatta acaagatctt ggatcgtttg
    20221 aggctgatta taaaaagcag gaattggtgg tatgatttgc acacctagtc gtgctaattt
    20281 agtcaagttt tccaagtgaa tggcatttaa tggcgtttca cgaggaacaa tgactaactt
    20341 tcgttgctcc ttcattgtta catcagcagc acgtgcaatc aggttgtcgc ctaaaccaac
    20401 agagatacct gcgactgttt tcatacttgc cggtacgatg atcattccat cggtcaaaaa
    20461 tgatccgctg gcgacagcag cacctaaatc tttgtttgaa taatggtaat caaacaggct
    20521 ttcgaattca gttagtgata agtcggattc cagctttaag ttctgttttg cccatggact
    20581 gatgattccg tgtgtttcta tcatcggata ttcttttagt ttcttcacta ggttgatggc
    20641 ataaatggtg cctgaagcgc cacttacacc aatcacgatt tttttcttcc gttgtgccaa
    20701 ttgttccatt ttccgcactc ctttttatga tgtcaaaaca gttgcagatc attcattatt
    20761 tcaaatattt ttcccaatct tctacttctt ggaattttgc acgtacaaaa tgttctttca
    20821 tatcgaaagg aactgtaccg tcaaagatag tttttgcact tatcccacgg aaacgaatag
    20881 agccaggatt atattctggt aattctgatg ggtccaatgg atggttcctc attcctggga
    20941 tcacgatgat atctttatct ccttggaaac gagtgttgaa agtccaaact acatcgttca
    21001 tgtcgaaaat atcaacatct tcatcgacta aaatgactgt cttcaattct ttgaaggctg
    21061 ataaagcaag aatagctgct tgacgctgaa taccttcatc cgcttcgttt tctttacgga
    21121 tttgcatgat ggtcatcaat ttaccaccac ctgcaggagg attgtagaca tttactactt
    21181 ttcctggaat agctttatcg accaaggcaa gaatacttgc ttctgtagga ataccggcca
    21241 tagatacgtg ttcttcactg ggaccaatcg ttgtttgcat gattggattg tcttttcggt
    21301 gagtaactgc tttgactttc actacttgta cagctggatt agcatcccca gtatagccag
    21361 gaaattctgg cattgctttt cctgagttcg tgttgatatc ttcttgcatt gtttcatttg
    21421 gcatgatttc tgcttcaatg atgaattcag cacgagcaat acccacttca tcgactgcta
    21481 taccatcaac gatttgaata ggttcgttac gcaaagctcc tgcacaccat agttcattgt
    21541 agccaagtgg agtggtaggt ggttcgaaag tcgtgccaat ggcaattgct ggatctaaac
    21601 caatattgat agtgataggc attggtttat tcaaggcttc gaattgtttt tggaagtgac
    21661 caatgtggcg tccgccaggc atgatgtaca taccgatcgt atctttatct tctagaacca
    21721 tacgatggat cgttacatcg ctcattgttt tttctggatc tgatcctaag actacaccca
    21781 ttgtgatgta tggaccagca tcttcaggag tatttgtagg tgcaggcaaa atgttgcgaa
    21841 tatcgaagtc tgcatcggta gctagatgaa cgacttcttg tgctggagcg tctgctttgt
    21901 ctactttcac aggtttgatt ggattttcta cagcgtcttt caagaaacga ccaagtgttc
    21961 gataatcatg gtgcaaaatt tcacctacac gtttacgact agccattgtt ccgatgttca
    22021 cacgtgtgcc ggggaaacct ttgatattgt tgaaagtcat agctggtcct tcttgagtcg
    22081 gacgttttac tgtaccgcca gcaccgatat aacggtaaac accagataat tctgcttcag
    22141 gatcgatttc aatatctgtt tcgtgatatt gacctggaag tgtttttagt tcttcgatga
    22201 cttttcttaa atcatacggt tgatctgtca tgagagattc ctccttagtt gtctttacct
    22261 atctatagta aaaggtgaaa aagaggaaaa caattcaact tgttcggtaa agtattctac
    22321 aatggaatgg atgcgttttt ttgataatag aataaactat gatatactta tcactaaaag
    22381 aaagcgcata cttagtgttg gaggaaggaa agtgaaaaaa atggaggagt catttcctaa
    22441 agcagtaaaa gtggaaaata tagcgaatat tttgaaagtt acatttgaaa acggagaagt
    22501 aaaatacgtg aaatctcatt ggacagaaga gataaccgat gccttgcaat ttggaaaaaa
    22561 aggaagagga aaaagaaaaa atcttctcgc actttctcgt aatatgtgga ttgggactga
    22621 agtaacgatt gaagcagatg gaacagtatt cataaatgga aaagacaggt atacgccaga
    22681 agagttgtgg tacaaaggaa agaaaagtat accagaatta taaaaaataa ttaaaaaaaa
    22741 cagcgcgaaa caaggaacat attacttcct cgtttcttgc tgttttctac tatttcagcg
    22801 ctcttatgac tcaaatctaa atgtggttgt cacaacctta ttctgcacgc aaagcgatag
    22861 cggcgtcttt tttcgctgcc attcgtgcag gaagatgacc gccaagcatc gtcaatatag
    22921 tagagacgat cactagaata atcgcatgga caggatttag ttgagcaaca ttttttagat
    22981 ccgtcatgtt gtataagatg ctattgatag ggaacgtagc tagccatgca ataaacacac
    23041 ctaatatccc agaagaaata cctaaaatac acgtttctgc gtcaaatacg cgagtgatgt
    23101 cttttttacg tgcacctaaa gcttttagta caccgatttc ttttgtccgt tcaatcacag
    23161 atgtataagt aatgatactg atcatgatca tacttgttac taaagagatc cctgcaaaag
    23221 caataaggac ataagtgata gcatccatca atccgccagt cagttcagtc atcgttccag
    23281 ctaaatcagt ataaatgatt ttatcttcat ctgatttgcc tttattgtaa tcatctagat
    23341 aatccaagat cttttctttg tcttcaaaat tgttcggata aatcatgata cttgaaggca
    23401 agctgtctcc acctaaataa ctaagcaatg tttgtttggc attttcgtca actttttcag
    23461 tagttaagac atttacacca ctgtctcttt gagctttgac gatttctgat tctttgtttt
    23521 cgttgacgat ttgagtcgtt aattgatcgc tataagcgat tcctggagat aagagattca
    23581 ttgtagaaga agatttcacg cgtaggatcc ccgagatctt caattcatca gaagcattct
    23641 gatacatcgc atcataatct gtattaggga taaaattccc agttggaagt tttgtatagt
    23701 aagtattgtt gtttactaat ttgaatgttg taccaacgat atcatcaaaa tcaagttttt
    23761 cgtcttcttt cacatcaaat ccaagattct tcaatgcgtt gatattagta ttattattgc
    23821 cgtctacgat cagcacgaca tctgttgctg aggctggata acttccggca agtaaagaat
    23881 aattgtcttt taaaaagttt tctttgctag tgtcaagctg tgtagggaaa cttgacactc
    23941 ccacacctgt cattgctgac atcgtactag ataaagaaag agattccgta tcaggatttt
    24001 gattcgagaa gctgacaggc tgtactttgc catttacatc acgtaaaaga ttgattcctg
    24061 tagtacgggt aaatccgata ttattactta agttaggatc gatgtctgtt acgtaatcga
    24121 cgtatgtttg atcaatctta ttcgtgtgct gtgctttatc ttcttcgctg acttttgcag
    24181 taatcgtttt gctgtctgga taatccgctt tgcttgcacc caatccggca tcgtcacgag
    24241 tctggttagt cgttacctta gaaatagtaa tcggaaattt cgccatcgtt tcagcttgcg
    24301 tgttatcgat ttgtttttgg aatccagaag aaagggacag aacgatcgcg attccgatga
    24361 tcccaatact ggaagcaaag gaggtcaaga aagtacgtcc tttttttgtt cggatattgt
    24421 tgaaggaaag ctttaacgct gtccaaaaac tcatctttgt tctgcgtaag ttaaattgat
    24481 catcttttgg tcgttcgatg tgaggatggg aatctgttaa gatcttacca tcagaaaatt
    24541 caatgattcg atctgcgtat tgatgggcca gttcaggatt atgtgtgacc atgatcacta
    24601 atttttcttt cgataactct tggatcaatt ccatgatctg gatacttgtt tctgtatcta
    24661 acgctccggt tggttcatcg cacaaaagga tatctgggtc attggctagt gctcgagcga
    24721 tggccactcg ctgcatttgt ccaccagata actgatttgg ctttttatgc atatgatttg
    24781 tcaagccgac tcggtgcaat gcatcttctg cccgttttcg tttttcgtct ttagaaacac
    24841 cacttaatgt catcccaagt tcaacgtttt caataatgcc tagatgacca atcaagttgt
    24901 aactttgaaa aatgaaaccg atagagttgt ttcggtaggc atcccagtca ctgtccttaa
    24961 agtctttagt agactttccg ttgattacca tatcaccgga atcataatta tctagtccac
    25021 caatcacatt cagcatagtc gttttaccag aaccactagg acctaaaatc gctacgaatt
    25081 ctttttgtct gaaagcgaca gacacaccat ccaatgcttt ggtggtggtt tcgccgactt
    25141 tataatattt ttttatatct tttaattgaa gcataatcac ccttctcccg tattgaaata
    25201 ttcggtatac taataagtat atcagaacaa tgatcgtagt atacgaaata aatgttaact
    25261 gaagatgaac tacaagaaaa aatctagaaa tttttcaaaa aacagaggga gatcaatgaa
    25321 tcgtatttta gtagtagaag atgatccgca attacagttg ctttaccgtt ctgtgttgga
    25381 acgagcgggc ttttcagtca tcaccgcatt gaatggagag gatgcgctga aaaaactaga
    25441 agcgacacgt atagaattaa tcattacaga tatcatgatg ccgaaaatgg atggctatga
    25501 cttgctagac tcattacgta gcaccagatt tgacacacct gttctaatga ttacggcaaa
    25561 ggccgatttt gaggataaga aacgcgggtt caaattagga gcagatgatt atatgacaaa
    25621 acccatcgat gtcaatgaga tgatcctacg tgtggaagca ttgcttcgac gttcaaagat
    25681 caattacagc agtagcttga taatcggaca aacaaaatta gagctagatg cttatacagt
    25741 gttacaagat cagcattcgt tgattctgcc tcaaaaagaa tttctgcttc tgtataaatt
    25801 actgtcttat cccaacaaga tcttcactcg ccaacagttg atggatgata tttggggatt
    25861 agatacgaat acagaagaac gaacgatcga tgtacatatc aaacgtttaa ggacccgttt
    25921 tgaaaagacg gaagattttc aaatcatcac agtacgggga ctaggctata aggcggttat
    25981 ttaaagatga aaaaaaagct attttcacaa ttatggatct attttgctag tattgtcttc
    26041 atttcgatct tagtgacact gctgtgtttt ctcggtttta ttttcttatt gtctcgacat
    26101 tctatctcgc ctgctgaatc ggctggaaaa ccaacgttat acccgttatt cgtctttgcg
    26161 gggttcagta tggtcgtggg tacaggaata tcgatttttg ttgggcggcg tatcctgcat
    26221 ccaatcagtg cgctagggac aaatatgagt ctggtagcaa cgggagattt ttcgattcga
    26281 atggatgaac agcagaaagt agcagaagta cagcaactgt ataaagattt caacgttatg
    26341 gtccaagaac tcaatagtat cgagacactg aggaatgact ttgtgtccag tgtatcccat
    26401 gaattcaaaa caccactagc aacaatccaa ggatatgttc aattgttaca agccccaaat
    26461 ctctctgacg aagaacgcca aatattttta tatcggatta tcgaaagtat cacgcagctt
    26521 tcacaattaa cagagaacat cttaaaattg aacaaactgg aaaaccagcg tatccaactg
    26581 gaaaagaaag agtatcgatt agatgaacaa atcagagaag taatcgtttt ttttcagcct
    26641 aaatgggaga aagaacagct agaattggat atcgaacttg ctgcagtaaa ttacacaggc
    26701 aatgaagagt ttttgtacca agtgtggctg aatatcatgg ataacgccat aaagtacaat
    26761 caaatcaatg gacagattca tatcaaacta tttgagacag ccacagagat tgtactggaa
    26821 gtgaccgatt caggtgtagg aatgaatgaa gagaccagag atcgaatgtt tgaaaaattt
    26881 tatcaaggag atacaagtcg tcaaatttct gggaatgggc taggactttc actggttaaa
    26941 aaaattctag aactgcatga tggacgtatt gattatagca gtatcgaagg agtaggaacg
    27001 acagcaatga tacggttaag taaacaatag gaaaaagaat aaaaccccta aaaattctat
    27061 tgaaggtttt taggggtttt tagtcgtttt tttgtgatta gggaaatgtt cttacggttc
    27121 ttttggcata aaaatcgctg ctttcttatt gaactacgag aaatgttaga ggtgtcattg
    27181 aaacaaggac atgatgcaag aaagagagcg aaagtcatgc gtcgctctct ttttttgggt
    27241 ggatagatgg acagaaaatg tagggcttag ttttcttctt gatttaaata ctcccaaaat
    27301 gtatctgaag ccaaagaatg ctcaccgact ttacgagtaa atgcaagata actctctgtt
    27361 gttggagaaa tagggagaat cacagtattt tcttttaata gattcttgac tgttttttcc
    27421 ataacaatag caatccccat gtcatttgca atcatattga tgatcaagtc gattcttgct
    27481 cctttataag agatttttgg ttcaaatcca gcttcgcgac acagatctag tacaggttga
    27541 tacaagttcg tagtggggcc aaggagcaaa aagttctcct ttttcagttc gcttagatcc
    27601 aatttctttt tactagctaa aggatgattt ttaggtagaa ctgctacaaa atcgtctgtt
    27661 tcagtaggca accactcaat cgaacttggt ggggcctgga aacttctagc aaagtggatc
    27721 gtgtcactat gttcattggc gtcattgtaa gttaattcga cactttccac ttctttcaac
    27781 tggactgtaa attcaggatg gcgtttcaaa aactcggtga ttttggaaaa acctttataa
    27841 ttgatcattg tcggtatcgt taagatattt aatgaaagat tgttgtcttt cgtataggtt
    27901 tcaagcactt cttgcatagc atggtattgt tctacgacat tttttacata tgggagcaat
    27961 aagcttccag cttcggtcaa ttcgattttt ctatgggcgc gttcgaataa tttgacgtct
    28021 aattctgttt ctaacgaaag aatctgttta gaaatatttc cttgggtggt gaattgtcga
    28081 tcagcagttt tggtgaaatt cattgtttcg gctaaatcaa gaaaaacttc gaatttatgg
    28141 atatccatca tattcctcct acttattcca ataaagaata ctttaacaca aaaaatgaat
    28201 tggtgtaatc ttctctgtct gttacaatct ttcttgcagc gaaagcaata attacagaaa
    28261 tttttctgat ttttttcgct tcgtagaaaa agagaggagt tttttgatgc caactggagt
    28321 tttgatcaat gtaggttccg ttttgtttgg cggactaatt ggcggattag taggaaataa
    28381 attaagtgaa cattttaaag cacagctgac gatggttttt ggtgtgtgtt cgatggggat
    28441 gggaatctat tccattgcgc cgatgaaaaa tatgccggct gttatttttg ctttagtgat
    28501 aggaacagcg attggtttga ttgttcattt aggtaatggg ataaataagg gggctgcgct
    28561 gatgcaagta cctatttcta aaatcttccc tagtgaaaag cttggtatga cccacgatga
    28621 atttatcagc acattagtga cggtcattgt tctattttgt gcaagtggta cggggatcta
    28681 tggaagttta gattctggga tgacaggaga cagtacgatt ttgatttcta aatctgtcct
    28741 tgattttttc acagcagcta tttttgcgtg taatttaggg tatgtcgttt ctgtgatagc
    28801 aattccccaa ttcattattt tttatatttt gttcttattg gcaaaattta tttatccatt
    28861 gactacacca gacatgatct tagattttaa agcttgcggc ggattcttga tggtcgctac
    28921 cggttttaga atgatcaacg tgaaaatgtt cccagtagct gacatgatac ctgcaatgat
    28981 cgttatcatg ccactgagct ggatgtggac aaattggatt atgccactgt tataataaaa
    29041 atcaaagaga agccattttt tgctgactaa aaaggatcaa atcttttagt cagttttttt
    29101 gtatttgaca aactgatttt gaaactatat tatatgttgt ataatataga ttgaaataaa
    29161 atattacact tgagaataga aggtgacgta atgcacatcc agattccaac tgttcttctt
    29221 gatggtactg tgctagcagc attaagcaag gaagatatgt atgggtatgc cttaacaaaa
    29281 atcgtacaag aaagtctctc tgtcagtgaa tctacactat atccggtttt gagaagattg
    29341 aaaaaagaag gatatcttga gacctatgat gaaccttttg aaggcagaaa ccgtcgatat
    29401 tactcgttga caccaatggg aagtcgtcat ttagaagaaa ttattgatga atggattgtt
    29461 tttcgagatt ctttagaccg gttattggag gagagcacaa atgaatgaat atttgaatga
    29521 agttgtggat catcttcaag aaattcctga agacgatcgt tttgatttga tccagtatta
    29581 tgaggaatat tttttagatt ctggaaaaac attagatcag atcattgatg aatatggtac
    29641 accaaagcag tttgccttaa aattaaaaat cagttacttt tcagaagaag aacaagcaga
    29701 aaacgagtta gtgaaaaatc caaaaagaca aattcgcctg atttggttaa tcattatcgg
    29761 tttatttgct tctcctgtcc tcattccact agcactgggg tttatattgg ttattggggg
    29821 actattggtt gctttactag cgctcatttt tagtatctat gttttatgta tcagtatctt
    29881 aggtgttggc ctattcatga tcatctcagg aatcagtgtg tttggacaat cgattctgag
    29941 tggattattc tttatcggtg tcggtctgtt cgccacagga agcgcagtat ttatttcgcc
    30001 actgttgctg aaaggaacaa aagggctttt ttatgtcatg atgaactttg ttaaatggat
    30061 gggacgacgt tttattacaa aaagagatct gcattcagcg ggcgtttgaa ggaggacaca
    30121 taaattgaga ttgaaacatt ttttgattac cggtattggt ctgatgctta ctggtggcct
    30181 cctagcaggt gcaacttata cattaggcgc tcaaaagagt ctgacttggg ataatggtcc
    30241 tcggttgatc gatatggtta ctgagacaaa aaaaatcaag gatgcaaaca aaatcgtcat
    30301 ccatgcagaa aatcaaacag tttcggtcat acgcggattg gattttgaga taaaaacgac
    30361 ttatgataag acagaaaagc cagaaataaa ggagaaaaat ggcgtgttga ctgtgaatgc
    30421 agtcaaaaaa tcaacaggtg caatcattag tttagaagac acaagtgcac aaatcactat
    30481 tgttgtccct gcagatcatg aattatctga attgacaatc gaagggaata atagtgcaat
    30541 tgatatcaac gaagtatcaa tcaaaaaagt gaatactaag acacatgatg gctgggtatc
    30601 gttcaatgaa tttacaggag gaacaataac tgcgaaagat gaattaggct cacttcgatt
    30661 agatgaggcc aatgtggata acttgatcgt cacaggtgaa aacagcaact tccatctaga
    30721 agacagtttg atcagcaaag aaggaaaagt catcctaaac ggtggcaatt tatctttgca
    30781 tgagatggaa aacagtggct atactatcca aacgtctggt gaatcttata ttgaaaaaga
    30841 ctaccagcca ataacaaata ctttgttaga acaaggacag aaaaagctaa agatcactgc
    30901 taaagacaca tcgatcaata ttacgacgga tcaagaggac aatgaaacag attgatcctg
    30961 tactcaactg gatattttaa gaaaaaacag caaagatcct tccaatcttt gctgtttttc
    31021 atcgcttaac gcacataact atagtaaaat aagaaagaaa atatctaaaa agaaaacagg
    31081 gtgaaagagt atatgaaggt agaagaaggt caaagagaaa agttaaaaac agaaatggaa
    31141 agattacata cttatatcac tcaactaagc caaaccttct atgatccaga caaagaaaaa
    31201 gtgatggtta attacccgaa caattcagaa ggtcgccaat tagagcaagt gtaccatgaa
    31261 gttttcaaac atttattgac ggtgaagaaa gagctggatt attattcttt accgattatc
    31321 gatacaggaa tattgaagta tgatgggaag aaggaacgat ttatctttaa atctgttcgg
    31381 gaagatttgc ctctttcagc agggatggat ctagaggtct tggtagaaga ctattttaca
    31441 gaagaaaagc actgggttcg aacaaaattg gattatttac cgcaggcagc cggagggaca
    31501 caggcaagcg gttggtacat cactgaagac aaagagttag aactagaagg tgtaatggcg
    31561 cgtattcgaa aaaaaaatta aaaaagtaac attagatatt tattgaagga taaaggagcg
    31621 caggaagtag catgagagaa aaaagagcat tttatatcaa tggttatgca gggattgtcg
    31681 gattggtcgt attagcttta gttggattgt ttttcttcta tttaggtatg tggcaagcga
    31741 aagtttgggc acttttttta agtatttttc tttggttgat cacgttattg ttgttaagtt
    31801 cggcaactgt agtcagtcct aatcaagcaa aagtaatttt gttttttgga caatatttag
    31861 gtacgattcg agaaaatggc tttttcctta cgataccttt ggcacaaaaa atgactgttt
    31921 cgttgaaagt tcggaatttc aatagttctg ttttaaaagt caatgatttg gatggaaacc
    31981 ccatcgaaat ctcagcagta gtagtcttta aagtcgttga tacagcaaag gcattattcg
    32041 atgtagcgta ttatcaagat tttgtagaaa tccaaagtga gacagccatt cggcatatcg
    32101 ctagccaata cccgtatgat acattcaatg agaatgattt gacgcttcga ggaaacacga
    32161 cggcagtgtc tgacgagttg caaaaagagc tgcaagagcg tttagcagta gcaggtgtcg
    32221 aagtaatcga aacacgcttg aaccatttag cctatgcaac agaaatcgct agtgccatgc
    32281 tccaacgtca acaagcaaaa gccattttgt ctgctcgaca aaccattgtt gaaggagctg
    32341 tcacaatcac tcaaatggcc ttagagcaaa tcgaagatgg acaagaaatc aatttcaccg
    32401 atgatcggaa agtccaattg atcaataatt tacttgtctc aatcattaca gacaaaggaa
    32461 cgcaacctgt tatcaataca ggcgacatga aagaccgtaa gtgacatact ttttgtaaga
    32521 taggaagaag gagtttagga cggattattg tcttgaactc tttttttagt tgttttaaag
    32581 aaaatgacaa aattgaaaga agaatgatag ttgattcgaa ggcagaattg ttcgcttttt
    32641 tctataatca gtatataaag taagagagaa ggggaaaaag tgaaaaaact tctgatcggt
    32701 ctacttgttt tcatcgttgt ggtacttgca ggggtgaaaa tcgcagatta cgtagttatg
    32761 ggtggagaag aatactatgt acaaattacg acagatggta caaaaacagt ttcaaaatca
    32821 gactctggac aagaaacagt caactataga tatatcctgc ctggctatac gaaagagggt
    32881 aaggaaaaag aactggactt caagggacaa ttgactcgac cgcttcgtaa aggagcatat
    32941 ttaaaaatca cctggaacaa aaataaaggt gtcacttcgt atgaagaagt cgaaaaaaaa
    33001 gacgtgccga aaacagcact ggaaaaacta attaaagggt agggaaagaa atgaacaaaa
    33061 tagtcgaagt acagaatgta acaaaagtat acggaaaaaa caatgagaaa aagacgcaag
    33121 cattaagcgg catcagcttc gatgtagaaa aaggcgaatt tattgggatc atgggcgctt
    33181 ctggttctgg taagtcgaca cttttgaata ttttatccac attagataaa ccaacagatg
    33241 ggcatatccg aatcaatcaa aaagatgtga ccacattaaa aggaaatcaa ctagcagatt
    33301 ttcgtgcgaa tgagattggc tttattttcc aagatttcaa tttattagaa agcttgacag
    33361 cacaagaaaa tatcgctgtt cctctttctt tacaaggcgt cagaccaaaa gaaatcaagc
    33421 aacgtgtcca aaaaattgca gaacgtttat ccatctctca tattttggaa agctatcctt
    33481 ctgagatttc cggtggacaa aaacaacggg tcgccgccgc acgtgcattg atcacacaac
    33541 caacgattct attaggggat gagcctacag gtgcgctgga ttccaaaagt gcccgggatc
    33601 ttttagatac gatggacgaa ttgaatacaa aagatcatgt atcgattctt cttgtgacgc
    33661 atgatccgtt ttctgcaagt tattgccaac gtattttatt catcaaggat ggcggtatcc
    33721 atcaagaagt gaaacgtgga gatcaaagcc gtgattcctt ctatcgagaa atactatcta
    33781 ttcttggaaa cttggaacag tagggggaga ttgactatgc tttggaaatt atcacttact
    33841 gggatcaaga gccgtttaag agactacttg gtgttattct caggattagt tatggcatct
    33901 gctatttttt acatgtttga atcaatggca agcaacgaag cttttttgaa aagtaatacg
    33961 atcgttgcgg ctacagttat cattttccga ttcggttctg ttttactagg gatcatcaca
    34021 ttcgtttata ttttatatgc aaattccttt ttgatgacga tgcggcaaaa agactatgca
    34081 atgtttatga tgctgggagc aaaaggcaga aaaatcgcac agatgatttt ttctgaaaca
    34141 ttcttagtag ggatcgcagc tactttagtg ggttcagctg tcggtgtagg attgacaagt
    34201 atcgtccacc gattattagt ggatcagtta gatctcaata tttcgcattt tacacctttt
    34261 agtattcacg ggttgttgat cactgttttg ttttttgctg ttttgttttt attagctgct
    34321 gttgtcaatg ctttttcgat tgtcaaaaaa ccgattttag cattgattcg tgcggatcag
    34381 acaccaacaa gaatcaagca gcataaattc ttgtttttcc ttgaagtagt actgggaatt
    34441 gcttttctag gctgcggtta ctatatgatg aaacacgtgg ggacctatca agtctttggt
    34501 ataggggtag ccttagttac gatcgtatta ggaacttatt tcattttcca ttctgtgatc
    34561 attttctttt tatcattact aaagaaatca gaacagattt cattgaaaaa attgaacaat
    34621 ttcacattgt cacagttaaa ctttcgtatt cgtgaatata cacaaatgct ctcaatggtc
    34681 gctatgctgt ttgccttggc actgggcgct ttgacggtag gattaggttt taaaaatgaa
    34741 attgcagact tgacagaaaa caattctgga tatgatctag tgatgaataa tcctcgggca
    34801 gaagatcagc aaaaaatcaa agaactttca ccaacacttt ctgcttctta tactcaaaaa
    34861 gaagatgcgg gaacgattta ctataatgca agtgaatttg cggctcaacc attgatcatg
    34921 ataaaacatg aagatggaac accgccgaag atcacgaaag aaaaagttcc tgctaaaaaa
    34981 atgaatgaac tgactgtaca agaggaactt cgaagctatg aattgccgga acagcaagaa
    35041 aaagaaatca aggtagtcag tcaatcagaa tttgatcaac tgaacttgcc acaatccact
    35101 ttacagctag ttcaggtaaa agacttccaa gcaaacctga aaccaatcga aacacttgtt
    35161 gctcaaaata aaatgaacaa tccatctttg caaaaagtag agtacagcaa ccagaaatat
    35221 gaaatgtatg aagtgtataa tggtctattc tctggttttg aatttatggg attcttccta
    35281 ggtttagctt tcttgaccat gcttgctagc tgtttgatgt tcaagatctt gtcaggaagc
    35341 aagagtgaca ttgctcgtta tgaaatgctt gaaaagatag gaacacgacg cgggttgctt
    35401 aaacaatcga tccgtagaga agtaggcgtg ttattcttag caccaggaat cttaggtgta
    35461 gtccatgtct tattcggatt acaaatgttt ggtctattca tgcaaaatcc atacaaagat
    35521 atttgggttc cgtttacgat attcttcgta ttgtatttca tctactatgt actgacgaca
    35581 tggttatata ctggaatcgt attgaaaaag agattgtgaa aaagctgtcc tgcatcaagt
    35641 aataagaaca gccaagcaaa aatagctcct catatttttc gtttggttgg gcttattacc
    35701 gcagatgcaa gcttttgaac accgtttgtt cggaaaaaga ggttgtgaca atatttttgt
    35761 cacaaccttt ttttcacggt cttttattag aatatagtaa attttttctc gttactcacg
    35821 gaataagctg ttaagaaaac ggtttccatg tatactggaa tcagtgcaaa tgaaagaaaa
    35881 gagggtgcaa ggaacatgga aatcaatgaa caaaattttg gcgaaaacta tcacttgatt
    35941 acgatcacga atcaaaatgg gacatctttg tctgtcagtg atcttggggc acgggttgtt
    36001 cattttcaaa caataatcga taatgaaaaa cgagagttga tcttaggttt tgatgaagca
    36061 aaagaatatc tagaaaaaga cccttatatc ggtgcttcaa tcggacgtat ggctggacgg
    36121 attacgaatg gcacattcaa attagatgat gaaacgttcg aagtaaaaac agacccggac
    36181 actggtcatt gtttacatgg tggtgcacct agctttgaag ccaagcagtg gcattataca
    36241 gtcatcaatg gtgaaaacga agcatcggtc attttctatc agactagccc agatatggaa
    36301 aacggatttc ctggaacttt agatgtagaa gtacggtata cgctgacgaa cgatgataca
    36361 tggcgagtga cggtccaagg gatcagtgac cgcaaaacag tattcaatcc tacaaatcat
    36421 gtctatttca atttaaccgg tgatccagca cagtcaatcg acgaacatat actttggttg
    36481 aatagccgat tatttgctac gctcaataaa gatacaacgc ctacaggaga agtagtttca
    36541 gtaagtggca cagcatttga cttccaacgt cctaaaaaat taggtgaggt ctttgccagt
    36601 gattttcgcc aatcaatgat agtgggcggc atcgatcatc catttttgtt gaaaaaacaa
    36661 tctggtttac taaatgctgc ggaattgaca agcccagatg aaaaagtatc gatccaagta
    36721 aaaacagatg cttcaggaat cgtgattttt acagcaaatt ttggagaaga tggaccggaa
    36781 atgcgcggga ataaattagt aaatcatggc ggtatcacat ttgagactca agaactgcca
    36841 ggagcggagc gacttgaaag ttttggagat gtcactctgg aacctaacca agtacgcgag
    36901 tttgtaacgg aatacaaaat aaatataaag aaatagaata aattaaa
//
LOCUS       DANKKG010000013        36565 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000013 DANKKG010000000
VERSION     DANKKG010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36565
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.013"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="PW004_000557"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="PW004_000557"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDL2725553.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="PW004_000558"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="PW004_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HDL2725554.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVDCREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="PW004_000559"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="PW004_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HDL2725555.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="PW004_000560"
     CDS             complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="PW004_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDL2725556.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="PW004_000561"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="PW004_000561"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDL2725557.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="PW004_000562"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="PW004_000562"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HDL2725558.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="PW004_000563"
     CDS             complement(8053..8523)
                     /locus_tag="PW004_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HDL2725559.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="PW004_000564"
     CDS             complement(8569..8994)
                     /locus_tag="PW004_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HDL2725560.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="PW004_000565"
     CDS             complement(9061..9330)
                     /locus_tag="PW004_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HDL2725561.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10932)
                     /locus_tag="PW004_000566"
                     /pseudo
     CDS             complement(9341..10932)
                     /locus_tag="PW004_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
     gene            complement(10947..14468)
                     /gene="mfd"
                     /locus_tag="PW004_000567"
     CDS             complement(10947..14468)
                     /gene="mfd"
                     /locus_tag="PW004_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HDL2725562.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14485..15045)
                     /locus_tag="PW004_000568"
     CDS             complement(14485..15045)
                     /locus_tag="PW004_000568"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDL2725563.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15398..16372
                     /locus_tag="PW004_000569"
     CDS             15398..16372
                     /locus_tag="PW004_000569"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HDL2725564.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16542..17942
                     /locus_tag="PW004_000570"
     CDS             16542..17942
                     /locus_tag="PW004_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HDL2725565.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18020..18742)
                     /locus_tag="PW004_000571"
     CDS             complement(18020..18742)
                     /locus_tag="PW004_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2725566.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18821..19327)
                     /locus_tag="PW004_000572"
     CDS             complement(18821..19327)
                     /locus_tag="PW004_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDL2725567.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19450..21249
                     /locus_tag="PW004_000573"
     CDS             19450..21249
                     /locus_tag="PW004_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HDL2725568.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21317..22891)
                     /locus_tag="PW004_000574"
     CDS             complement(21317..22891)
                     /locus_tag="PW004_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDL2725569.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23146..23655)
                     /gene="mreD"
                     /locus_tag="PW004_000575"
     CDS             complement(23146..23655)
                     /gene="mreD"
                     /locus_tag="PW004_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HDL2725570.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23659..24513)
                     /gene="mreC"
                     /locus_tag="PW004_000576"
     CDS             complement(23659..24513)
                     /gene="mreC"
                     /locus_tag="PW004_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HDL2725571.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24668..25306)
                     /locus_tag="PW004_000577"
     CDS             complement(24668..25306)
                     /locus_tag="PW004_000577"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HDL2725572.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25307..25957)
                     /locus_tag="PW004_000578"
     CDS             complement(25307..25957)
                     /locus_tag="PW004_000578"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDL2725573.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25970..26869)
                     /gene="rsgA"
                     /locus_tag="PW004_000579"
     CDS             complement(25970..26869)
                     /gene="rsgA"
                     /locus_tag="PW004_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HDL2725574.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27034..29103)
                     /gene="pknB"
                     /locus_tag="PW004_000580"
     CDS             complement(27034..29103)
                     /gene="pknB"
                     /locus_tag="PW004_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HDL2725575.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29100..29840)
                     /locus_tag="PW004_000581"
     CDS             complement(29100..29840)
                     /locus_tag="PW004_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HDL2725576.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29853..31211)
                     /gene="rsmB"
                     /locus_tag="PW004_000582"
     CDS             complement(29853..31211)
                     /gene="rsmB"
                     /locus_tag="PW004_000582"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HDL2725577.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31211..32149)
                     /locus_tag="PW004_000583"
     CDS             complement(31211..32149)
                     /locus_tag="PW004_000583"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HDL2725578.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32142..32633)
                     /gene="def"
                     /locus_tag="PW004_000584"
     CDS             complement(32142..32633)
                     /gene="def"
                     /locus_tag="PW004_000584"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDL2725579.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32648..35056)
                     /gene="priA"
                     /locus_tag="PW004_000585"
     CDS             complement(32648..35056)
                     /gene="priA"
                     /locus_tag="PW004_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HDL2725580.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35309..35614)
                     /locus_tag="PW004_000586"
     CDS             complement(35309..35614)
                     /locus_tag="PW004_000586"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDL2725581.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35619..36233)
                     /gene="gmk"
                     /locus_tag="PW004_000587"
     CDS             complement(35619..36233)
                     /gene="gmk"
                     /locus_tag="PW004_000587"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDL2725582.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
BASE COUNT         9886 a         7988 c         6705 g        11986 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gttttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaatc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc tcctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatgggat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctgcaacg tactttgata tgaactgcgg
    10741 aagaccggat aaagctaaag tcattgctat cccataaatt ggataaactt gctgataaac
    10801 ataaaaacct tcgtcgccga ctaagttttg gaggggaatt cggtaaaggg cacttaatac
    10861 ttttgctatg aaagaagcaa tcgtcaatac aaatgctcct tgcatgacgc gccgcatttc
    10921 tttatgatcc aaatcattgc tccttcctac gctgacagat atttttgctc acgcaacgct
    10981 ttgacaaact tttggatttc ctgaagccat actgcttctt ccatttggtt ggggatcgtc
    11041 aagcgtatca ccatctgttc tttttcaacg cctagactgg cttttagttt tgtcgctgtc
    11101 agtgcttcga ataactgttc cacactatag cgttttgttc caactttact tagagtaaag
    11161 actatatgcg cctcctgttt ccttatcgtt tctaggagcg ctcgatcgcc atccattttg
    11221 atctgcccaa ttgtcaagag atgtgctact tcttctggat attctccaaa acggtcgagc
    11281 agatcatctt ctagttcatc tagcatttct tgcgaatcaa gttcacgaat tcgtttatag
    11341 atctcgattt tctgccgttg atctgccacg tatgtttcag gtagatacgc atcgacaccg
    11401 aggtcgatct caacagtggt cttctccact tgactattct ttccttgttt acgagaaaca
    11461 gcttcgctta gcatttgaga atacatatcg aaaccgactg catcaataaa tccatgctgc
    11521 tgagcaccta agagattacc tgcaccgcga atggaaagat cccgcatcgc aatcttgaat
    11581 ccagatccca gttctgtaaa atctttgatt gcctgaagac gtttttcact gacttcgtta
    11641 aggatcttct gctgctcata cataaagtaa gcatatgcga cacggctgct tcttcctacc
    11701 cgccctcgca attggtataa cgtggataat cccatataat cagcgttctc aacgaacaac
    11761 gtatttgcgt tcgggatatc tactcctgtt tcaatgatgg ttgtagtgac aagaacatca
    11821 tattgacctt caataaattc aaaaagcgta ttttctaatt gcgcctctgt catttgccca
    11881 tgagcataac cgatccgtgc ttccggaacg agttcttgga tttcttctac tttttgttcg
    11941 atcgtttcta cccgattata taaatagaat acttgtccac ctcgtcccat ttctcgatgg
    12001 attgcttcgc gaatagcccc aggatttttt tccataacat acgtttggat cggataacga
    12061 ttcgcaggcg gtgtttcgat tactgaaaga tcccgcacgc ctaacatcga catatgcaaa
    12121 gttctcggaa tcggtgttgc agtcagagtc aaaacatcca cttgtgcacg taactgtttc
    12181 aatcgttcct tatgttttac accaaaacgt tgttcttcgt caatgatcag tagacccaaa
    12241 tcgctaaatt caatatcttt tgacaaaatt cggtgggttc ctacaacaat atcaacttga
    12301 cctgtacgca gttgttcgat ggtttctttt tgttgctttc ttgttctgaa acgacttaat
    12361 agcccgatat ttacggggaa accttcaaat ctttccagca tcgtttcata gtgctgctgt
    12421 gccaagatcg ttgttggaac taaaaaggct acttgtttgc tttcctttat cgctttaaag
    12481 gccgctcgaa gcgctacttc tgttttccca taaccaacat cacctacgag tagtcgatcc
    12541 attggttttt ctttttccat atcccgtttg atctccgctg cacttctcaa ctggtcatca
    12601 gtttcagagt aggggaaggc attttcgaat tctttttggt aggcgtcatc aggtccaaaa
    12661 gcatatcctt tttctgactc tcttgcggca tataatttga ttagatcgtc agcgatatcc
    12721 tcgatttttg aagagacttt tcgcttggtt ttcgtccatt cacttccgcc tagcttattg
    12781 atgcgaggcg ctttggcttc agaggcgaca tatttttgga tcagattcag ttgagtaaca
    12841 ggaataaaca gtttgtcgtc gttttgatac aaaatagtca tatagtcttg gtgaacgcca
    12901 tctacttcta gcgtttccat cccaatgtat ttcccgatac catgattggc atggacaacg
    12961 taatctcctg cttttaactc attatagctt ttcaatcgct ctgcgtttgt tactgtttga
    13021 cgtctcgttt gttttttcgt cgttttttgg aaaatttctt tttctgtgat agctactatt
    13081 ttttcttgag gaagttcaaa accagcctgc aaagctcctt caactaattg tacctgtcct
    13141 ctgatcaatg catttttttc tgtttctact actaatatgt cttcatctcg aagcatttgt
    13201 tctgctttgc gtattctgtc ctttgtcgga ataaagatca caacagtctg tttttgcttg
    13261 cgccaacggt ccatttctgt tttcagcaat ggcatttgtc cgaagaactg ttgcatcgtc
    13321 cgatattgaa aattatggat tgcttggaaa cgaagatttc ccatcccttt ttggaagagt
    13381 gagaaaaagg ttgtggtgaa cttttccttc tgcacgattt tttgtatatc tgctgaaaat
    13441 gtctgctcag aaaacacccg caattcgctg attttttgag tctgccattc tgccgcttcc
    13501 ctttcgattt cccgatttgt ttccatgatt cgctgataat catcaacgac tagtaaacta
    13561 tttgaaggga aataatccaa gatcgtcaca gattcttggt aaagaagatc tgagtagaat
    13621 tttgcttctt ctgtcgggac acctgcttgc cagcttgaag tcagctgacc gaagtaatct
    13681 tgcaaaaact cgcggtctgc ttcttctgtg gtcgcagcaa gccgtttttc tagcattttt
    13741 tgcatttgct ctattccatg ttctagatcc tctggtccgt agacttgttc actcgttggg
    13801 gaaacccaga tttcttccgt tttttcgatt gagcgttgtg tttctgcttc aaaataccgc
    13861 atagaatcta tctctacatc aaatagttct acacgaacag gataggttgt cgttaacggg
    13921 taaatatcga taatgcttcc tcgaatactg aattctcccg gttttgcgac catctcttct
    13981 cgtacatagc ccatcaaagt caattggcga ggaagatttt ccacgtctat ctcatcacca
    14041 atatgccaat tcaattgcgc ctgtagccaa gttttcttat tcggcagtcg tttgtgcaaa
    14101 gatgcgactg gcaacaaata gatacctgct tttttttctt gaagtgcgtt aagcgttgca
    14161 actctctcgc ctcgcgcttc tggagaagaa aaggccattt cagcagaaag cacttcatcc
    14221 actggaaata aatggatctg atcttgcgga atcacatgtt gcagatcttc tgctaactga
    14281 ttcgcataat acaaatttgg catcagaata atcatgtttt tatttgtttt cttaaacgct
    14341 cgagcaaata gaagtgtttt cgctgagccc gctaatcctg tgattagttg tctaggcatt
    14401 ttttcttcta aattgctgac ccattctttt acttgcggat cttttccgac taattgaata
    14461 atatccattc cttttcctcc ttcctcaatt gaatcgattc atcgcatcca cgaacgaatg
    14521 cccttctatg agatattcga ctgctttaac agattgacac atgctttctt ctatcaaagg
    14581 ctgattttct ttgctgaaag gactaagcac atgctggact actgtttttt tcccttctgg
    14641 cctgccgatt cccaccttga ttcgatcaaa gacattcgta ttcaaatggg aaataatact
    14701 tttgataccg ttatgcccgc cggcacttcc cttttgacgg agccggattt ttcccactgc
    14761 cagatccaaa tcatcgtaga ttacgactaa ttcctctgga taaatcccaa aataagtcat
    14821 tagtgggccg actgcacgac cagattcatt catgaatgtt tgtggtttga ctaaaaggat
    14881 cttttctcca ttatgaaaaa attcggcaac ttctgcttcg aacggatttt ttttgaaagt
    14941 agcttgatgc tcgcgtgcaa ttttatccac gaccataaag ccgatattgt gttttgtata
    15001 ttggtatttg gtacctgggt ttcctaaccc gacgatcatt ttcatttttg ttttccacct
    15061 aattctaaaa agcagcacaa aaggctggac tgctaattag cggtccagtc catcgagctt
    15121 ttttcttttt tatacttgtc aatgcatagt ataacacaaa acaaggcgtt tcggctaaaa
    15181 gagaatcgtt aataaatgat aaagaaatta aaaatttctt caaagtttaa tgaaataatt
    15241 gatatataaa aatagtgaaa taacgcacat gtaagctttt acatataata caaccttgtt
    15301 atctgttata ttttatagcc aaatagcgtg acatggggct tgagaaatgt tagcataagc
    15361 atgggaaaaa acaaatcaat ggaaggattg atactaaatg actgcagctg cagaaaaaaa
    15421 agatcatcaa aaagttattc ttgttggaga cggcgccgta ggttctagct atgcctttgc
    15481 tctagttact caaaatatcg ctcaagaagt aggtattgtg gatatcaata ctgctaaaac
    15541 tgaaggagac gcaattgact tgtctcacgc tttggcattt acttcaccta aaaaaatcta
    15601 ttctgctact tacgcagacg cacatgatgc tgacttagtt gttattacag ctggcgcacc
    15661 acaaaaacca ggtgaaactc gtttagactt agtaaacaaa aacctaaaaa tcaaccgcga
    15721 tgttgtcaca caaatcgttg aatcaggatt taacggtatc tttttagttg ccgctaaccc
    15781 agtagatatt ttgacttact caacttggaa attctcaggt ttccctaaag aacgcgtaat
    15841 cggttcaggt acctctcttg actcagctcg tttccgtcaa gcacttgcgg aattagttga
    15901 cgttgatgca cgtaacgtcc atgcttacat cttaggtgaa cacggagatt cagaattccc
    15961 agtttggtca catgcaaatg ttgccggctt gcaaatctat gaatgggtaa aaaacaaccc
    16021 agaaatcgat gaagaagcta tggttaacct attctttggc gtacgtgacg ctgcttacac
    16081 aattatcgag aaaaaaggcg ctactttcta tggtatcgca gtagcattag cacgtatcac
    16141 tcgtgccatc ttagatgatg aaaatgctgt tcttccttta tcagtattca tgaatggcga
    16201 atatggattg aacgacatct acattggtgc acctgctgtt atcaaccgtc aaggtatcca
    16261 aaaagtaatc gaaattcctt taagtgacag cgagcaagac cgtatggctg cttctgctaa
    16321 gcaattaaaa gaaattctag atgaagcttt tgctaaacta gacgctgaat aatctttgaa
    16381 catgacggaa aacatctatt cggtgttttc cgttttttta ttttatcacc cttatggttt
    16441 ctaaattttt tcataaagta gagataacct tcgtcttttc ctctaaatta tgctatcatg
    16501 agaccgttcc accctaaact tttacttaag gagttgtcga tatgacaaga tcttcgcatc
    16561 gaaaaaaatc gaaacatgtt gctttgcttt cagttcttgg tgttctctta gtgctgatca
    16621 gcttttatac ctatcgcagt acatactaca caaagcattt tttgccgaat acagaaataa
    16681 atggaataaa tgtcagcaat ctaacagtcg aaaaagcaaa tgaaaaatta aaagaagctt
    16741 attctgataa aaaactggct attaaagaag atggaaaagt ctggcaggaa gtaccaaaat
    16801 ccgagctcgg ctacaaagat gatttcactt cggagctttc tccgatccta aatgaacaaa
    16861 acggatggac ttggggaatg acttatgtct cagcggcaga aaaacaagaa atagatcctg
    16921 tcagtcaaaa ccaccaaaaa ctagacacta ccgttcagac attgacaact aagctgactg
    16981 atttgaacaa agaccggaca cctactactg atgcaacgat cgaaaaagca ggtgactctt
    17041 tcgttatcaa gcctgaagtc aatggaaata cgatcgacgt cgatgctgcg gtcaaacaat
    17101 taaaaagtgc ggtaaactct ggtaaagata caattgaatt gactgaattc aaagaaaaac
    17161 caaaagtaac atctgaagac agctcactga aagaacaact cgcatccatg aatgctatcg
    17221 caaatgtaaa agctacttac agtataaacg gtgagacttt ccagatccca tcaagtgata
    17281 ttatgagttg gctgacctac aatgatggaa aagtcgattt agatacagaa caagtacgtc
    17341 aatatgtaac agatttaggc acaaagtata acacaagtac gaatgacaca aaatttaaaa
    17401 gtacaaaaag aggagaagtt acggtcccag ttggcactta cagttggacg atccaaacag
    17461 acagtgagac agaagcattg aaaaaagcga ttctggcagg ccaagatttt actcgttcac
    17521 ccattgtgca aggtggcaca accgctgatc atccattgat cgaagatacg tatatcgaag
    17581 ttgatctgga gaaccagcat atgtggtatt acaaagacgg aaaagtggcc cttgaaacag
    17641 atatcgtttc cggaaagcca actacaccta cacctgctgg agtcttctat gtttggaata
    17701 aagaagaaga tgcaacttta aaaggaacaa atgacgatgg aacaccttac gagagtccgg
    17761 taaactactg gatgccgatt gactggacag gtgttggtat ccacgattca gactggcaac
    17821 ctgaatacgg tggagactta tggaaaaccc gcggttcaca cggctgcatc aacaccccac
    17881 caagtgtaat gaaggaatta ttcgggatgg tcgaaaaagg aacgcctgta ttggtcttct
    17941 aacaaaaata atatataaaa agaaagcacc cgtacgattg gtacaggtgc tttcttttta
    18001 ttatctaaca atgtgctact tacatttcag cagcagtaaa gacttctgaa acatcatcat
    18061 cgtcttctag cttatctact agacgttcca attgctcttt ttgttcctca tttaaatcaa
    18121 tcgtcgtttg tggaatcatc gtcaattctg cttgcgccaa agtgaactct ttttccaact
    18181 catctcgtac agcagcaaaa tcttctggtg cagtataaat ctcgaatact tcaggagaag
    18241 tttgcagatc ttctgccccc gcctctaata catcctcaaa catcgtctct tcatcaacag
    18301 tcaacccatc gcgttcaata gcgatatatc ctttgcggtc aaacaagtaa ctgactgaac
    18361 ccgtttctcc cagtgatcca ccgtttcgag taaaagctac tcggacattt gtcgctgtcc
    18421 gattacggtt atctgtcaat gcatggacta agattcctac tccacccgga ccatatcctt
    18481 catacgtgat ttcatcgtaa ttcgtttcat ctacggcact cgtagccttt ttaatcgccc
    18541 gttccacatt gtcgttaggc atatttgcag cttttgcttt gtccacaaca agccgcaaag
    18601 ctggattcat agatggatca ggaccgcctg ctttagctgc catataaatt tcacgagaaa
    18661 gtttctgaaa gactttccct cgttttgcat cttgggcatt ttttcttcct tggatattat
    18721 gccatttact atgtcctgac atgatttttc ctcccttgtt cttttgagaa tacacttcta
    18781 ttctagtact atttcacaaa aaaacaaagc cacagccaat ctataaacgc ttcttgaatg
    18841 ccgcaaaaag cacacataat atcacagcag tcagagcacc gatcgaatcc aacatgacat
    18901 cttggaacat aggtgttcgt ccaccggtca tcatctgatg gaattcgtcc aaacctgcgt
    18961 accctgttgc tgaaagccaa gcaaggatcg ctgtcagcca ccaaattttt agcttgggga
    19021 aaacacctaa aaacaagctg cctcccaaaa taaaataggt accgaaatgt gcgcccttcc
    19081 ttaaaaagaa ctctaaaaat ttggcgtagc ccagatggtc gatactcacc atacttccac
    19141 cataattaaa acgtataccc gctaatgtgt ctttaaatgg ctgattaggc agccagcttt
    19201 ctaatcgcga aacttgtgat tgctgtccgt aagtttgaga agaactgtaa aataagatta
    19261 taacgactat cagtgcgatt actaaataaa tatttccgtt tttcatttgt ttttttgaag
    19321 aattcataag tcacctctgt attttatctt agactttttt tgaagaaaca acaatcattt
    19381 tcatagagac ttcttccata aaaaagtgct atacttaatt ttgtattaca atatgaaggg
    19441 agttttttca tgagttattc attaaattgg aatctagatt ctattttttc cggaggcagt
    19501 cattctgatg ctttgaacca acggatgaag caattggaag accaaatgaa cgagtattat
    19561 caccgcgtaa caaaatggtc acctagttct gacaatgcag agcagttaaa cgctattctt
    19621 caattgcaag aaacgattac gaatggtttt acccaatgca gcagctatat tactgcattg
    19681 ctttctgcca atgtcaatga ttcagatgca aaggtcttat caggcaagct ttacgcaatg
    19741 ctgcctcgtc tgcaatcagc cgaaactgta ttgtcaaaga aatttgcaga aatatctgac
    19801 aatgattgga atcagatgct ttctcaatct tcgtttgaaa caattgcttt tcgtttaaac
    19861 gagattcgtc gtgatggctc acaattatta tcagaagctg aagaaaacat tataaatacg
    19921 ctttctttag atggtctaaa tgcttggagc agtcattatg atacgattgt agccagcatt
    19981 tcgattccct tcgaacaaga tggacaagta gtcgaattat ctgctggaca agccttcaat
    20041 aagatgatgg gcgatccaga tccaaaagtc agagaaacac ttttcgcagc atgggaagaa
    20101 gcctggaaag aaaaggcacc cctcttcacg gatactttga atcatttaga tggttttaga
    20161 ctttctgact atgaacttca tggtgtcaca gattttctac aaaaaccatt agagtacaat
    20221 cggttgaaga aagaaacatt gtctgtcatg tgggatacaa tccagaaaaa caaacaaccc
    20281 cttgttaatt acttgactag aaaagctaat cttttcggta aagaaaaaat ggaatggcaa
    20341 gatcaagatg cacctattat tttaggcgat ttgaaagaaa aaacctttag ttttgacgaa
    20401 gcagcagcat ttattatcga aaactttaac aaattcagtc caaaaatggc taaatttgcc
    20461 caatctgctt ttgaaaagag ctggatcgaa gcagaagatc gtcctgggaa acgtcctggt
    20521 ggttattgta ctgagttacc agaaacaaaa gaatcaagga tctttatgac ctacagtaat
    20581 tctgttaatg aggttgcgac acttgctcat gaactaggac atgctttcca tagcagcacg
    20641 atgtgggatt taccttcttt aaatcgcgaa tatgccatga atgtcgcaga aacagcaagt
    20701 acattcgcag aactgatcgt agccgatgct acattaaaag tcgcaaaaac aaaagaagaa
    20761 aaaatcaatt tgctagatac aaaactgcaa aatgctttag ctatgttcat gaatatccat
    20821 tcccgattca tttttgaaaa tcgattctat gaagcacggc aagaaggatt agtctcagaa
    20881 gataagatca cagagatgat ggttgaggcg caaaaagaag gctatcaagg agctttagct
    20941 acttaccatc catatttctg ggctgcaaaa ttgcatttct ttattgatga cgtaccgttt
    21001 tataacttcc catacacatt tggctacctc tttagtttgg gcatctatgc ttacgccaac
    21061 caaaagggag caaattttga agaagaatat atccatttac tacgcgatac tgcctcaatg
    21121 acgacggaag atcttgcgaa gaaacatttg aatgtcgatc ttacccaacc agatttttgg
    21181 caagcaggta tcgacatggt gactgaagat atccaaacgt tcatggaatt gacagaagaa
    21241 tttatctgat ttctatttta tcacaataaa aaaagagtta gtccagaatg ggctaactct
    21301 ttttgattaa tcattcttac atgctgacag caaagtcagg ggcaaaccat tgaactgaac
    21361 caacttttac gctttcgcct ggttgaggag catggatata ttgtcctccg cctaaagcaa
    21421 tcgctacgtg gtaagttccg ccttgtgacc cccagaataa caagtctcca gcttttgctt
    21481 gagaaactga aatttttgtt ccagctgatt cttgaggaac tgtccaacca ccgatgtcac
    21541 gaccagttac ttgcatgtaa acatagcgtg tgaaccctga gcagtcaaag ccacttggat
    21601 ctttaccgcc ccaaacatat ggagtaccaa tatatttgta agcttctgct acgattgctg
    21661 caccattcac acttccagaa ggagctggag caggtgttac aggttttggt tgttcaggtg
    21721 ttggtgttgg cgttggagcc ggagtactac ttcctgtgcc gtttcctgta tcaacacttt
    21781 gatctgttga tggtgttgta ggcgcaggtg ttgttgattc ttctgttgtt gaagattcag
    21841 gcactgttgt tgattcttct gttgctgaag attcaggcac tgttgttgat tcttctgttg
    21901 ctgaagattc aggcgctgtt gttgattctt ctgttgctga agattcaggc gctgttgatt
    21961 cttctgttga agattcaggt gttgttgatt cttctgttgc tgaagattgt gtagctgatg
    22021 atgattcttc agtcattgaa gattgtgtag ctgatgattc ttctgttgct gagctttgtg
    22081 cagtagatga agcttgagta gcttgtgctt cagcctctgc ttgttcgcgt gcttcttttt
    22141 cagctttttc ttgtgcagct tgttgacgag cttgttctgc tagacgttgt tgttcacgaa
    22201 tacgagcttg ctctgcttca gcttctgctt tttgacggtt caagtcagct ttcttgtctt
    22261 cagcagttgc ttgttcagca gctaatgaag ttttcagtac gttcaaatca gcttgtttag
    22321 ataaaagatc gcctttttga gattccaaag cagcttggtt ttcagctaat tcttttagtt
    22381 tagcatcgtt ttcagctttt ttgtcttcta cagctttttt atcttgtttt tgttgttcca
    22441 tcaagtcgtt gtttgctttt accatcgttg tcattgcttg aacgcgtcca atagcatctg
    22501 ctaatgaatc agcattcaat acagcatcga tgtagtttga gcttgtgttg cttacttgtg
    22561 cttcgcgtgc ttgtttttgg attgtatctt cacgtttttc aatacgttct tgtaaatctg
    22621 caatgtcttt tactaattgt gcagattctt gacgcaatgt atcttgtttt gctaataggt
    22681 cttgtgcttg tgtgttgatt tcagaaactt ggctttctaa ggcatcgatt tgtgattgtg
    22741 catctgcttg ttgatttttc aagtctgcaa tcttttgatc ttgctgttgt atctgagaat
    22801 caaaatcgtc cgccgcggca gcgatcggtg atgctacggc agtcaaggtc attgaacata
    22861 ccattactgc tgatattaaa ctctttttca ctcttcattc ctccgactgg cttaatttaa
    22921 taaatatcta aatatattta agtctttctt aacgatataa aaattgtacc atagcagtgt
    22981 gtcaacgata taatagttat gttacaaaaa tgtttcaaaa caacttttaa aaatgacaaa
    23041 actgttcgtt tcagaaaatg atcatgaaaa agccgcgtta agtctagcat ttcttaagat
    23101 aacaacgatt ctttttcgct ttcgacctaa aaataaaaaa agaaattatt catcactgaa
    23161 taatttctta aagggaaaaa tgaataatac aaatataatc atgttcaaca gcagtgtagg
    23221 tcctaaaaac cttgtaataa aatacgtgtt atttactact gctaatttaa aaatcaactg
    23281 gaccaccatc gtaaacaatt cataacccgt gacaaagatg atcatactga aaaattcagt
    23341 aaagatgttg acatggataa cgtctttcat tacatacatc aaccatacaa ttaaagggag
    23401 agctactgca tagataccga taacgccaat atagtaagcg tcatagatag ccccgagaac
    23461 aatcgtggta atcagtaaat aacgtttttc aaacgccata ctgcaacata ataatgccaa
    23521 tatcagaaag tgggcatttg acatataggt gcctttcgac cactccccta gcattcttgt
    23581 taaatgacca tctatcaaca tcaaaaagaa caaaacgatt ggcagatagt acttcattgt
    23641 ttctttcttc aacatggact aatcctccgc tagccgttgg acaatcgtca caactgacac
    23701 gtcatacatt tctgcataag gtttcacata aacttcccga tccagcccat aactatctgg
    23761 tttcgtttta ataaccgtac cgatcggcaa gttagctgga gaattccctc caagaccgga
    23821 tgtctggaca acatcccctt ctttgatatc cgtatcacca gttaattggg tcaccactaa
    23881 ggcatggagc ttttcatcat agtttttaag caaaccaaac gcttcgccat tagccgaaga
    23941 tacccgtact ggaaaatgat tggagctttc attagaggat gacagtaatt cgattttaga
    24001 cgaagccgta ttgacctcga tcactcggcc aatcaatcct ttttgcgaca tgactgccat
    24061 atttgcttcg ataccgtctt tagaaccctt gtctacgatc agcatgtcct gccaactgtc
    24121 aggcgaacga gtaatgacat ttgccgttac tttttcgtaa ctagtcagcg tctgattcaa
    24181 ctcaagttct tttttcaacg cttcgatttc tctctcgtaa ttcttgtttt gctgtgaaac
    24241 gtcatcatag ctatcgattt tttcttttag ccgctcattt tcggcataag ttgaaaaaag
    24301 attatggaca gaatctactc catttcgtac ccaccgtact ggggcagaga ttgttttatc
    24361 aaccattgca acactgtcat taacgatgga ttggaagaaa ttgggctttc cttcattcgc
    24421 tcgctgcgca gcggtcacac ttagtatcgt caccacgatg ataacaataa ccaaggtaat
    24481 aataatattt ttattaggat tgaacttctt cacaaagaca cctcgaatta attagactct
    24541 tgtgttcaat tttatcacta aatgccgtga aacaaaaggt aagttgctcc tcttaagctt
    24601 ttttttagca aataatagat tctgtcattt ttagcctgaa agacaaattt ttttatttgt
    24661 ttatggatca atctttgctt tgaataactg caatcatacc atctgaaaaa gaaaattctg
    24721 cggtatctga aagaaattcg ttacttgcat aagatgtcgg atattctact tctttatttt
    24781 ccaatacata cttgctgcct tttaatgtaa gattcgagac gggagtcaag cagcaataag
    24841 ctaaatactt catatctggt atctgtctta ctatatgctc tccaggcaaa taataagaca
    24901 aataattttg acaatctctg ataataagct gatgggcaaa gggtttgaag cgaggttcca
    24961 tacctaacca gagattggct aaaagatgat ccaatcttcc gcccgtagct ccaatcaaag
    25021 tgacttccgc ttgcgggtaa cgttcaaatg tttttaagat acctaactgt gtatctgtat
    25081 cgtctttttc tgcaggggaa cgggtatact ctttggcttt tgcaaatact tccttctgct
    25141 cttcgttcga caatgaatca aaatcgccca ctgccagatc tactgggtag cctgcttgaa
    25201 gcaggtacag cgcaccacgg tctatcccga cataggcatc gtatttggac agcagagact
    25261 gcggccagtt ttgcggatta cctccagcag ctaataaaat tttcatttag tctaatgcct
    25321 cttttaatcg ttcgatttgt ccgactggat tttcggctcc atagatatat gatcctgcaa
    25381 cgaagacatc cgcccctgca tctttacaga tttttgctgt ttctggaacg attccgccat
    25441 ctacttcgat tgcataagtc caattgttct ctctacgtag atcagcgagt tccgtaattt
    25501 tttctactgt ttcttcaata aagctttgtc cgccgaaccc aggattgaca gtcatgacta
    25561 gtacttgatc agccaaaggc aaaacatgtt tgatcatgga gactggtgtt cctggattga
    25621 taactactcc agctttaact cctaagcttt ttgtcatttg aagtgcccga tgaatatgag
    25681 gagttgcttc gacgtggaca gtaatgatat ctgctccagc ttttgcaaat gctggaatgt
    25741 agttttctgg atcaacgatc atcagatgga cgtctaacgg taatttagta acaggacgga
    25801 ttgcctgaac aatatttggc cctaatgtga tatttgggac aaattggcca tccattacat
    25861 ctacgtggat gtaatctgcg cctccttttt ccactaactc aatgtctctt tgtaaattgg
    25921 caaaatctgc actcaaaatg gatggtgcta tcttcattct tgtttcctcc tattttcgtt
    25981 taccttcttt ttttgcatac attggttttc ttttttcgat ttcttgtaag aattgcaaat
    26041 aattgtcgta acgtgtctgt gcgataccac ctgtttctac tctatgtttt acttcgcagc
    26101 caggttcttt agcatgcatg cactctctaa atttgcaatg gacagaagct tctacaaatt
    26161 caggaaattg tctcggcagt tctcctgctt caattgtcaa aaagtcaatc gagctaaatc
    26221 ctggtgtatc tgctactaac ccgccataga taggaatcag ttccacatga cgtgttgtat
    26281 gccgtcctct tccaagtgca tcagaaatct ctcctgttgc taaattcagt tccggtgata
    26341 tttgattcag tagcgtcgat tttcctgctc cagactgacc cataaagacc gatagccgtt
    26401 ctgggaagta acggatcagt tctgtcgtat cacccgtatg attgggtaca atcaccggat
    26461 agcctatttt agtataggtt tgcttgatct cttcgatttt ttctgggtcg tctgacaagt
    26521 cggtctttgt caaaaaaata atcggttcga tcgactcgta ttccaaggta actagaaagc
    26581 ggtcaagtag attataagaa aatgcaggct ctacgatact tgtgacaatg attccaagat
    26641 caacatttgt gactggtggg cgaactaatt gattttttct cggcaagatt tccagaagat
    26701 agccgtctgt ttgattttca ctctcaaaga tcacctgatc tccgacaagc ggcgtgattt
    26761 tccgattccg gaaatttcct cttcctcttg tttgataggt ctctccgttt gcgtatacat
    26821 agtaaaaacc gctgattgct tttctgattt gtcctttgat aagtgccacc tccgtttttg
    26881 tttattttac catacgaaga ggaaagtagg tgttattcca tgcaaacttc tcaaagtata
    26941 tggcttgaaa attgatcaaa agagatagaa acacaaaaaa gagaatgcaa caaatcaatg
    27001 tcgcattccc ttctgatata ttttattcag cacttatttt gaactttctg aggtgctagc
    27061 tgatgaactt gtggagctgg aagattcttc ttttgaagag ttgcttgtgc tactggagct
    27121 acttgagctg ctagaactgt ctgaagagtc tttttcagaa ctagaagacg ctggatctgg
    27181 tcctttagag taaattacgt tcacaaccat tcctttggta attgtcgttc ctgcccctgg
    27241 tactgtacga atcactttat ctttatccac cgtgtttgaa tattcggaag tctcatgccc
    27301 gatatattct gcaccgacac tagctaaata actttgtgct tcgctttttg tatagccgga
    27361 gatatcattc aatttaggct ctgttccttg gcttacgaca aaattgatgt aagtttcttc
    27421 tgctacaact tcacccgagg ctggatcctg actgatgatc gaacctgatg gtacagtgtc
    27481 gctatagtca taagtagcac taatattatt gatattgaat ccttgtgtaa caagccaatt
    27541 acgaacagtt tcgtagtttt ctcctacatg actactcaaa gtcactgttt ctggtccttt
    27601 gctgactgtg aaagtaatcg tgtctgtttt aggatcgact tctgtatctt ctgatggaga
    27661 ttgcgaaatg atcattccgc tttctacctc tgaactgaat tcctcttctt tttgaatcaa
    27721 gtcagcagaa aaccctaatt ctttcaaact tgcttcggca tcttcataac ttttaccact
    27781 gtaatcatct aatttgattt ttttcgtgcc gctactaata tacagagtga cttcgcgttt
    27841 ttgcttgact gtcgttcccg ctgccgggtc tgtcttcact acattacctt cttcgatttt
    27901 gtcatctgca atttcttctg tttttgttgc tacttctaat ttcgcttttg cgagtgtttc
    27961 tctagcagaa gcttcagaaa gatcagaaac atcaggaaca acaacttctc ctcgcccgcc
    28021 agaagcaaag aatacagctg ttccgattcc taaaacagca agaatagcta ataggatcag
    28081 ccaccatttt ttctgtcctt tctttttagg atcaggcaga acttcttcct cctctggcaa
    28141 ggtttcgttt tctggcttgt cttttggcag aggcatagat ttgaatgaat caggcattgg
    28201 tgcatcttcc gtaaggggtg tgataacttt tgtttcatta tccatcacat gaggctgcca
    28261 tttttcttcg tttgcacgag ctggtgataa tactgtagaa agatcatcag acatttcctc
    28321 ggctgactta taacggtctg caggttcttt agccgttgcc cgtaaaatca cgttttccag
    28381 tgcttgtggc acatttggat caagtgcttt tatagatgga agatcatctt gaaaatgttt
    28441 taaagcaatt gttacagcag attcaccatc aaacggtacg ctacccgtca acatctcata
    28501 aaggatgatc cctaaagcat aaatatctga ctgcttcgtt gccatacttc ctctggcctg
    28561 ctcaggggac agataatgaa cagatcccag catcgtattc gtctgcgtga tcgatgtttc
    28621 agataaagcg atagcaatcc cgaaatccgt gatttttaca acaccttcat tatcgatcag
    28681 tacgttttgg ggtttcaaat ctcggtggat gatttgatgg ttatgagcta aagaaatagc
    28741 agaaaggatc tgctgcataa tatttactgc tgtttcataa ggaacaggat aatgtgtttg
    28801 gatatatcgt ttcaagtcca ttcctttgac gtattccatc accagatact gcatgttgtc
    28861 ttcttcacca acatcgtaaa cactaacaat gtttggatga acgagttctg ttgctgctaa
    28921 agcttctctc tgaaatcgtc gaatcgcagt ctggtcattt tgaaagtcaa atcgtaagac
    28981 tttaactgct acatcacgat ccaagattaa atcgtgcgcc aaaaatacat ttgccatacc
    29041 accgctaccg atatttccag tgatttgata acggccattt aattttttgc ccaactcgat
    29101 catacactgt cctccttata ttcgataaga agaacggtga tattatccgc tccgcctgct
    29161 tcattggcac gatcaatcag ctcagttact ttatgagaca atgggccttc ttgattgatg
    29221 atcgttccta tttcttcttc agacagcatg ttcgtcaagc catctgaaca taacagcagt
    29281 cgatctttta gttgccaaag atgagtagaa acatctactt cgactgttcc aggcatgccg
    29341 acagaacgtg ttaaaatatt tttccttgga tggttcactg ccatttcttc gctgatttct
    29401 cctgatttga ctagttcatt gactaaagaa tgatcctccg tcaattgcat gattttatct
    29461 tcacgaatca aataagctct gctatcccca acattggcaa tcgtgaacca gtcatctgac
    29521 agagcggccg ctacgattgt tgttcccata ccgttatatt ccggctgttc ttgtccacgc
    29581 tgataaatca gcatattttc ttcttgaatc gattgaatga accattgggc gattttttca
    29641 tcgtcaacag cttcttgttt ttcccaagct tcgcctagat tcgttaccgc catctgactg
    29701 gcaatgtctc cggcacgatg accacccatc ccgtctgcta atacagcaag cttgagacct
    29761 gaacggctgg taaatacact ggcatagtct tgatttgtgt tacgtcgtct tcctacatct
    29821 gattgatatt caatctgcat tttttcacct cattactttt tgcgcaaaca acaaatgaaa
    29881 aatccatctg tgtaaaactg atgcggatac aaagtcaaca ttttctcttc gattgatgat
    29941 tgtagcatag gattggtcaa aacatcaatc ctctcaaaat cagggtgtct ttccaagaaa
    30001 gcagaaacaa cgtcttggtt ttcttctttt aagatcgtac aagtgctata agtcagaata
    30061 ccggaagact ttaaagttgg tgcgcagctt tctaaaattt ccaattggat ttttggtaaa
    30121 ttcgctaatt catccggctg ttttttataa cgaatatctg gttttcgccg aagcagccca
    30181 agacctgaac aaggcgcgtc tactaaaatc cgatcaaact gctctgcttc gaactgttta
    30241 ctgacttctc tagcatccat cttttgagcg tagacagcag aagagacacc tagacgtgct
    30301 gcattatctt caatgagttt gactttgtgt tcgtgtacat ctaaagcgac cacttctcca
    30361 ccgcatgacg gatctaaaaa agaagcaata tgcgtcgtct tacctccggg tgctgcacag
    30421 gcatctaaga catgatgttc aggctcgatt tgcatagacg gcgctacaag catcgaactt
    30481 tcatcttgga tcgtcatgac gccatcgtgg aacaatgagc tagaagctaa atgccctttt
    30541 ttcgctacta taccgtatgg actgacttgg cttttttcag ctgtgatctc ttcttcttgc
    30601 aaaacagtga tggcctgatc tctggaaagt cggcgtgtat ctactcgggc actagcatgg
    30661 ctaggctgat atagcgataa accgagcttt tcagtttctt cgtatccgat ttgatccacc
    30721 agtcgtttcg tcatccatag aggcaggctg attttgacag aaagtctttc gataggatca
    30781 gcaatgcctt gcagatctgg tgctccttga cgctgataat tcctcaagac accatttaca
    30841 aatttccctg tcccaggatt tccttttact tttgcgattt cgaccgcctc gtttaaaata
    30901 gcatgtgcag ggactttatc taaaaattct aattggtata cagaaagata aagaagcgtc
    30961 ttgacccagt catccacttt ttttgcttta gcgatcaatg gtgcaacgta aaattccaat
    31021 agtcgctgat ggctaatcgt accatagatc aattctgtaa ataaacccac atctttttga
    31081 tttagctcgc tttgtttgat catctcattc aacaaaagat tcgagtaggc gccacccttg
    31141 ttcactcgct ctaatgtcag taatgccata tatcgtactg attttttcat ttttttaggg
    31201 atttttttca ttatttcacc tgctgaccaa tctgaacttg ctgaccgctt ccgttaagga
    31261 aatcatgtac ggcctgtttt cctttaccag ctggctgtaa ttcattgatg gctaagacgg
    31321 tctgtttacc gcaagcaatc catagatttt ttttgtcttt ccggatgatc gtaccaggtg
    31381 ctttgtctgt agtttctgcc aatacttcaa ccgataataa tttccagcgt atgccctcat
    31441 aggtcgtgaa tgcaattggc caaggacgca ttccgcgtac ttggttgtca atttcttctg
    31501 cagttttatg ccaatcgatt gcttcttgtt cccgtgtgat gtttggcgaa aaagttgctt
    31561 ctgactcgtc ttgaggtact ggtttttgtc catccaaaat attgggcaat gtttctagca
    31621 atagctttct tcctaaaagg ctaagctttt caaacatcgt ccctacatca tcttgtttag
    31681 tgattggtat gctttcttgc gcatagattc cgcctgcatc catttttttg atcatttcca
    31741 tgatcgttac accagtttct ttctccccat tcatgatggc ataatgaaca ggcgcacctc
    31801 cccgatattt aggaagtaaa gaagcatgga cattgattgc ccctaatttt ggtacttgca
    31861 ataacttttc aggcaagaat tggccaaatg ccgcagtgac gatcacatca ggcaccaatt
    31921 caatgatttc ttccatttct ggcgaaccgg agattttctc tggctgcagc acacggatac
    31981 cgtgcttcag agccgcttct tttaccggtg tcggcgtaat tactcgtttc cgcccaactg
    32041 gacggtcggg ctgtgttacc actgcctgga tctcgtaacc tgattctatc agcccttcaa
    32101 gaatcggcac agaaaaagca ggtgtgccca taaatactag cttagtcatc aagatgttcc
    32161 tccatatatt cttcaagttc ttcttccggg atctgctgta tcattttctc aatgaacaag
    32221 ataccgtcta agtgatcgat ttcatgctgg aaggcacggg ctaaatagcc aaaagccgtc
    32281 acttctatct cttctccgtc tcgatcataa taacgcacgg tcacttcatc tgctcgcttg
    32341 accgttccat agacatgcgg aatactgaga caaccttcta cgtcaaggct ttcacctttt
    32401 gcttcgatga tttctgggtt aatcagttca aatttctcgc cttcatctac ttcgatcaca
    32461 gcaattctct tgttttgtcc tacttgagga gcagcgatcc ctattccgtc attcgcaatc
    32521 attgtctcat ataaattgtc taataaagcg attgtctcgt ccgtgattac atcgattggc
    32581 tgagcagttc tttttaattt atcattaggg tggatcaata taggatagcg cataatttgg
    32641 ccctttctta aataaagttt aatggttcgt tgtcaattgc cacaaaaaga ccttgtcgct
    32701 gttctttctg gcttccattc aaaatttcat ccaaaacatg aggaagattc ggttcatgtt
    32761 tatatttgat gattagttga taataatatc ggtttttcac tctcaaaatc atgctgggcg
    32821 ttggccctaa tagcaagctt tgagggctta acacttcttt tagctgattg gcaatctgaa
    32881 agattttctt tgccgctact tgttcttcag gatggctggc ggttattttc acagtaaagt
    32941 aataaggagg atagcctcca cgatgccgta catgcatttc ctgctgataa aactgctcat
    33001 aattttgttt tttagctaat tctatggcgt aatgttgagg attgaatgtt tggatcacga
    33061 cttcgcctgc tttttccgct cgccctgctc gtcctgaaac ttgggtaagc aattggaatg
    33121 tatgctcact agaacgaaaa tccggtaaat tcagggaggt atcagcattc aacaccccaa
    33181 ctaatgtcac ttctggaaag tccaaacctt tagcaatcat ttgtgtccct aataatatat
    33241 ctgcttcttt tgcaccgaat ttttgtaaaa tctgttcgtg tgcccctttt cttcgagtgg
    33301 tatctacatc catacgcaaa atcctagctt gcggataaag ttctcggagt tcctcttcta
    33361 ctttttgagt acctgttcca tagtagcgga ttttattgcc gccacaatta ggacagcgat
    33421 tgggaatagg ttcttcatgt ccgcaataat ggcagcgcat tgaacgtgta tccatatgca
    33481 gtgtaagaga gatatcgcaa ttaggacatg gaagaacaaa tccacagtcc ctacacatca
    33541 caaaagacga atatcctcga cgattgagca gtaggacagt ttgttctttt ttgtctaatc
    33601 gattttgtat tttttcctgc aaattagcag aaaaagtaga ggtccgatga ttctgaaact
    33661 cttcacgcat atctacgatc tcaatagctg gaagctgtgc agctgctttg gctcggtggt
    33721 ttaactgcaa taactgataa acgccttttt gagctcgtgc tcttgattct aaagagggcg
    33781 tcgcgcttcc tagaactatt gggcaatgat gatatttact gcgccaaatt gctagatctc
    33841 tcgcatgata acgcggtgtc tcatcttgtt tgtaacttga ttcatgttct tcatcgataa
    33901 tgatcagccc gatatttttc agcggggcaa aaatagcaga tcgtgcgcct actactactt
    33961 gcgcttcttc ccgctcaatc ttgcgccatt catcatattt ttcaccttgg gaaagtccgc
    34021 tatgtaagac cgcaactgat tctccgaagc gtcctttgaa tcgctcgacc atttgcggag
    34081 tcaatgcgat ttcaggcact aacataatag ccgttttatt ttctgacaaa acatcagcga
    34141 ttgcctgaag atatacttct gttttcccgc tgccagtgat tccctccagc aaaaagacct
    34201 gatctttttg ctgctgacct gctgtgatga tttgttcgac agcatttttt tgttctgcat
    34261 tcagttccaa tgccgtcgtt tggtcaaata ctcggtcttt ataagggtcg cgataacgtt
    34321 ctgattctat aaactccagc cagccctttt cttttccttg attcaagaca gcagtactga
    34381 ttcctttatt tttgaaataa gcaattggtt cttcttggct cagttcagat aaacacaaaa
    34441 gaagttcttt ttgtttcttg gcattttgcc gtaattttgc ccattcttct tcgatttgct
    34501 tttcagttaa caaagaacgg accaaacgaa tcgtttttac tttatttcgt gtatggactt
    34561 catatttgat atctactaat tgctcttggc gccagcgaat caattgagaa aggctgcctt
    34621 cttcttctgc ttccttccag gaaatcactt ctcgctgatc aaacaatacc tgaacttctg
    34681 gtgtgtctgc caaaggatag atcaatttat catattctgc tttcatcaca ctcggaagca
    34741 tcgtttgtag acaagtgatt ttgaatgcgt aagtaatttt tttcatataa tcagctaatg
    34801 aaagtaattc ttcattgacc accggagcaa ggtctagcaa acgaataact gccttcaaag
    34861 atggattagc tgattcttct gatttttgga tagccataac aaatccttgg acatgacgat
    34921 tgccattacc aaaagggact tctactcgca tcccgacttg caaagctgtt tccatttcag
    34981 agggaaccaa ataagtgaat ggctgatcgg tctgcatagt aggtacgtcc acaatgactt
    35041 cagctgttaa tgtcatggtt cgtttcctcc tttttttgtc ttttcttatc aagcgacaaa
    35101 aagttccttg cctattttac tagagatcga tggatttgtc ttatcttctg ccaaatccta
    35161 acaaaaaatt aacagcagat aagaaaaaaa agaactgtgt caaaaagttc agacacagcc
    35221 ttttctacca gacaataagt ggcgaatgtt tgaaaaggaa acagctgggt ttgcttttct
    35281 ttttagatgt tcgctctatt tttctttatt acattttttg ctcttcacgg atacgtgatt
    35341 ccaattcgtg ttgttcacgc tctttttgtg cttgacgttc ctcttcttcc atcttcaaac
    35401 gttcacgttt taattcagga tgtggatcat tgactacatt tcctgcatcg atttcttcta
    35461 aagcttgacc gacgcttttt actgaatcaa aagaatctag agtaggctgt gcacttgcat
    35521 ccaattcatg cgcgcgttta cttgctaaaa tcactagaga atattttgaa tttacacgat
    35581 ctaatagtga gtcaattgaa ggttttaaca tcataagact acatctcctt taacatttta
    35641 atatatttac cgattacgcg atctactcga aaatgttcac tggcaataat gtttttgatt
    35701 cgttcaactg ctagcggcac ttcatcattt acaacagcat agtcgtaaag agccatcatt
    35761 tcgatttctt cttttgcaac tttcattcgt tcttcgatta cttcatctgc atctgttcct
    35821 cggcctacga tccgtgattt taattccgct aaatctggcg gagtcaaaaa gatgaataca
    35881 ccatcgggca ctttttcttt gacttgtttt gctccttgga cttcaatctc caagaaaaca
    35941 tcttttcctt catccaatgt cttgttgata tatgacaaag gagttccata ataattcccg
    36001 acatattctg cgtattccag catctctcca gcttcgatca ttgattcaaa ctcttctttg
    36061 cttcggaaga aataatcaac gccttcgacc tccccttcac gcattttacg ggtagtcatt
    36121 gagatcgaat actgaaaatc attatcctca ctctcaaaaa ttgcttttcg tactgtgcct
    36181 tttcctacac cggatggacc agacaacacg attaataatc cacgctctga catgacgacg
    36241 tcctttcaac tagtcttttt gtacttatcg tttattttgc actttttttc tgcaactttc
    36301 aaggttatct ttaattatag ctagaaattg gtaaaaaagc taccctgtac caagaggtcg
    36361 taaaaaagct gtcctgcatc aagtaataag aacagccaaa cataaacagc ttctcatgtt
    36421 tttcgtttgg ttggggttat tatcgcagat gcaagctttt gaacgccgtg tataagaggt
    36481 cgtgaagaag ccgattagtt tcgagtatga agaaaaattt tgtgaaaata gtttctcaca
    36541 tttttgcata aatttggctt aatac
//
LOCUS       DANKKG010000014        35947 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000014 DANKKG010000000
VERSION     DANKKG010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.014"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            126..578
                     /locus_tag="PW004_000588"
     CDS             126..578
                     /locus_tag="PW004_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2725583.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(702..1553)
                     /locus_tag="PW004_000589"
     CDS             complement(702..1553)
                     /locus_tag="PW004_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HDL2725584.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(1566..2351)
                     /locus_tag="PW004_000590"
     CDS             complement(1566..2351)
                     /locus_tag="PW004_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL2725585.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            complement(2703..3644)
                     /gene="ribF"
                     /locus_tag="PW004_000591"
     CDS             complement(2703..3644)
                     /gene="ribF"
                     /locus_tag="PW004_000591"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HDL2725586.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            complement(3648..4571)
                     /gene="truB"
                     /locus_tag="PW004_000592"
     CDS             complement(3648..4571)
                     /gene="truB"
                     /locus_tag="PW004_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HDL2725587.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            complement(4939..7301)
                     /locus_tag="PW004_000593"
                     /pseudo
     CDS             complement(4939..7301)
                     /locus_tag="PW004_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            7749..7922
                     /locus_tag="PW004_000594"
     CDS             7749..7922
                     /locus_tag="PW004_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2725588.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            complement(8079..8426)
                     /gene="rbfA"
                     /locus_tag="PW004_000595"
     CDS             complement(8079..8426)
                     /gene="rbfA"
                     /locus_tag="PW004_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HDL2725589.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(8453..10759)
                     /gene="infB"
                     /locus_tag="PW004_000596"
     CDS             complement(8453..10759)
                     /gene="infB"
                     /locus_tag="PW004_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HDL2725590.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            complement(10772..11083)
                     /locus_tag="PW004_000597"
     CDS             complement(10772..11083)
                     /locus_tag="PW004_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HDL2725591.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            complement(11080..11373)
                     /locus_tag="PW004_000598"
     CDS             complement(11080..11373)
                     /locus_tag="PW004_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HDL2725592.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            complement(11395..12570)
                     /gene="nusA"
                     /locus_tag="PW004_000599"
     CDS             complement(11395..12570)
                     /gene="nusA"
                     /locus_tag="PW004_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HDL2725593.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            complement(12593..13066)
                     /gene="rimP"
                     /locus_tag="PW004_000600"
     CDS             complement(12593..13066)
                     /gene="rimP"
                     /locus_tag="PW004_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HDL2725594.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            complement(13211..17569)
                     /locus_tag="PW004_000601"
     CDS             complement(13211..17569)
                     /locus_tag="PW004_000601"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HDL2725595.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            complement(17781..19490)
                     /locus_tag="PW004_000602"
     CDS             complement(17781..19490)
                     /locus_tag="PW004_000602"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDL2725596.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            complement(19558..20826)
                     /gene="rseP"
                     /locus_tag="PW004_000603"
     CDS             complement(19558..20826)
                     /gene="rseP"
                     /locus_tag="PW004_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HDL2725597.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            complement(20987..21787)
                     /locus_tag="PW004_000604"
     CDS             complement(20987..21787)
                     /locus_tag="PW004_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDL2725598.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            complement(21784..22596)
                     /locus_tag="PW004_000605"
     CDS             complement(21784..22596)
                     /locus_tag="PW004_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HDL2725599.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            complement(22791..23348)
                     /gene="frr"
                     /locus_tag="PW004_000606"
     CDS             complement(22791..23348)
                     /gene="frr"
                     /locus_tag="PW004_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HDL2725600.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(23351..24073)
                     /locus_tag="PW004_000607"
     CDS             complement(23351..24073)
                     /locus_tag="PW004_000607"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HDL2725601.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(24209..25090)
                     /locus_tag="PW004_000608"
     CDS             complement(24209..25090)
                     /locus_tag="PW004_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HDL2725602.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(25189..25971)
                     /gene="rpsB"
                     /locus_tag="PW004_000609"
     CDS             complement(25189..25971)
                     /gene="rpsB"
                     /locus_tag="PW004_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HDL2725603.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            26330..26809
                     /locus_tag="PW004_000610"
     CDS             26330..26809
                     /locus_tag="PW004_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HDL2725604.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            27026..28117
                     /gene="phnW"
                     /locus_tag="PW004_000611"
     CDS             27026..28117
                     /gene="phnW"
                     /locus_tag="PW004_000611"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HDL2725605.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            28110..28238
                     /locus_tag="PW004_000612"
     CDS             28110..28238
                     /locus_tag="PW004_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HDL2725606.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            28242..28787
                     /locus_tag="PW004_000613"
     CDS             28242..28787
                     /locus_tag="PW004_000613"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HDL2725607.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(29241..30932)
                     /locus_tag="PW004_000614"
     CDS             complement(29241..30932)
                     /locus_tag="PW004_000614"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HDL2725608.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(31352..32299)
                     /gene="arcC"
                     /locus_tag="PW004_000615"
     CDS             complement(31352..32299)
                     /gene="arcC"
                     /locus_tag="PW004_000615"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDL2725609.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(32414..33433)
                     /gene="argF"
                     /locus_tag="PW004_000616"
     CDS             complement(32414..33433)
                     /gene="argF"
                     /locus_tag="PW004_000616"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDL2725610.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(33524..34753)
                     /gene="arcA"
                     /locus_tag="PW004_000617"
     CDS             complement(33524..34753)
                     /gene="arcA"
                     /locus_tag="PW004_000617"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HDL2725611.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            35214..35915
                     /locus_tag="PW004_000618"
     CDS             35214..35915
                     /locus_tag="PW004_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDL2725612.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
BASE COUNT         9792 a         7659 c         6318 g        12178 t
ORIGIN      
        1 aataaaagtt agaattttct gacttgattt ttacaatgac cttaattaaa atcgttcttt
       61 ctacatttcg tgattaagca aatatcaact aagtcagtag aaccattcac ataggagggt
      121 gaacattgaa agatttttac tcagtaaatg aattagccga acaattaggt gttactacac
      181 gttctatcag aaattatctt catgaaggaa aactaaaagg aacaaaggtt ggcgggcaat
      241 ggaaattttc tgaacgtaat ctttttgagt tcctatacgg agatcaagca gaggaagcag
      301 caaaagaaat gcagcgattc atgctaaatg caccaatcac gatgcgtttc aaccttcaat
      361 atcgtgattt tacagcaatc aatcaattcc gagaacaatt agtccagtac cataacgatg
      421 tttacgcaaa taaaaaagac cgtcttttac aatacgatct ttataaagac aatcatgcgg
      481 agatactaat cggtggtaac ttcaattatg tcgtaaactt tagtcaatgg atcaatgaaa
      541 aattattgat ccagacagat atttcccttg tatcttaaac aattaaaaag cgctagaaat
      601 tccagagatg ttggaaattc tagcgctatt tttttataag tacttactgt ttggttcaaa
      661 caaatatttt tttaataaat gaaattttac catctttatc ctcaaataaa gaccgtgacc
      721 atgcttccta ctacgatcag caataatccg ataatcgtta attttttttc ttttggtgtc
      781 ttattttcat gcaggatgta cataccgcct aatgtagaga taactacgtt tagctgtgta
      841 atgatatatg cagtagcaac cccattttca tttgctgaaa aaatataagc caatgctgac
      901 acactaaaga tggctccaac gatcgttgct ttccaactct tgtcttcaga aaatgcggtt
      961 ggtttacgga aaatcacata aatcactgcc cctaaaaaga caccgatcat ttgcggaaag
     1021 aaaatggaaa gtccgcttgc atcaactagt ttaggcaatg cactgtatac ccaataacca
     1081 atacttgttg atgctaatag actaattcct tttgtgatac cgcctttttt tccaggatca
     1141 tcactgattg ccgttaaagc agaaccaaat acaagaagaa tgattgctgc tgctccaatg
     1201 attttcccca tacttccaga ccattctcca aaagcgaaga caccaatcaa cgaggttccg
     1261 actaactgta atgcagtcga aattggcatc gttttagaaa caccaatcaa gttgaaagat
     1321 gtatactgtc ctgtttgtcc aataacccaa aatgcaccag aaagtaaact aatcaaaaat
     1381 gtagtcactg aaataccagc tggagaagcg aataattgaa cgagtaagcc gacaagcacc
     1441 gctccaatac cagttcctaa tatctcattt gttggtctgc caccaatctt tttcaaaatc
     1501 agtggctgga tcccccagcc aactgccggg atcaatgcaa tcaataaacc catttttatt
     1561 cacccttatc cttttccacc ttggaaagaa ggatataaag tcatcccacc atctgcaaat
     1621 agtgtaatac ctgtaacata actggattcg tctgaagcca accatgcagc tgtcgccgct
     1681 atttcttttg gttcacctac gcgtcctagt gggatcatac tttccaagag ttctttttgt
     1741 tcaggatcat caaattttgc cgcattaata ggtgtacgga tagcaccagg gcctatacta
     1801 ttgattctga ttcccttcgg tgcatactcc attgctaccg tttcggtaaa taattttacc
     1861 ccgccttttg aagcagcgta atgagcaaac gttggccatg ggatctgttc atgaacagaa
     1921 gacatattga tgatatttcc ttttttatta ttctctgtaa agtaatttaa agccgctttc
     1981 gttccgataa aaacgccagt caaattgact tttaacactc tttcccaatc ttcaaggcta
     2041 agttcatggg ttggatgctg attttccatc ccagcattat taacccaaac gtccagtgtc
     2101 ccaaaatttt ctaaagccgt atcaagtaaa cgctgtattc cttcttcaga actgacatct
     2161 gcttgtacag agactgcttt tccgcctgcc tcttcgattg ctttgactgc tttttctgct
     2221 ccttctttat ccgagttata gttgacaact acattcattt tttctttccc gaatcgttga
     2281 gcaatcgctg ttccaatccc ctttgagcca ccagttacta ctgcgacttt gttttctaat
     2341 tccttgtaca tagtacccac tcctttttca tacctaaacc tgttattacg gataactttt
     2401 atttccgcct taaagtgagt gtacaccaag atatatagtc taaagcaaat gatacgctct
     2461 agcaatcgtt ttcatgaatt gctagtgtcc ttttatttct ataaagaaag aggttgtgac
     2521 agaagtggtc agcttcaaga aatttcggct tatttccgaa gaagctactt ctgaaaaacc
     2581 atttatttgg aaaaaaaggc tgtgacaaga cttttgtcac agcccctttc ctgggcttat
     2641 ttatcaccca ttttattgta attgctagtt atacataaac aagagatttt atgcataatg
     2701 ttttattgct gagaaaaata ttcagctgtt gcttgttcat cctccttgag ttgttcaatc
     2761 aattcctcta cactagaaaa tttcacttga tctcttaaaa attgattcca gcgtaccgcg
     2821 acatgttcac catagatatc ttgattaaag tccaaaatat acaactctac agtcagctcc
     2881 cgtccttcac cgaatgtatc attatgtcca attgaaccca tggctggata ccatgtttcc
     2941 ccaattttta tttccgacac ataaacacct tctttaggca aatgaaccgt gctctttact
     3001 ttgacatttg ctgtagggaa tcctagtagt ctgcctcgtg catcgccatg gacaactacc
     3061 ccttctacct catatgtgca acctaatagt tttgctgctt cttttacttg tccgctgtct
     3121 agtaatccac gaatacgggt agagctgatt ttttcacctt gttcttcttc tttagaaact
     3181 gtgacgattt tgaatcttcc tttggcatat tcaggaaaat gagcgacatc tgcaatgtct
     3241 tttggtccat acgtataatc aaaaccggaa acagcatatt ttgcatgtaa ccctacgatg
     3301 tattgatcaa caaattcctg cggtgctaaa tgagcaaaag aagaagtaaa atcaacttca
     3361 tataaataat ctatacccaa agcggccatt ttttcttctt tttgcttaag gctagtcaaa
     3421 tatttcacgc tgctaggatc gatcttttta aagacaatag atggatgctg attgaaagtc
     3481 atcaatgcta atttcaatcc ttctttttgt gcaatttcca tacctgtctt tatgactttc
     3541 tggtgtccga tatgcacgcc gtcgaaaaat cccagcacta aaacgacatc gtcttctgga
     3601 atttgttctg attggtaagg atgtctgatc ttgatgatat tcattcgtta ttcctcattt
     3661 ctcaatactt tactaggttt taatttgttt ttttctttcg ggtttggctg ataaatactg
     3721 acaacttttc ctttataaaa caaagcaatt tcttctgacg gcatttctgg gagcgcaaat
     3781 acagtataat caagtctcat accgtttttt actttctgcc atacttcatc agtaatatct
     3841 actcgtttga accgttccac tcctttttct attggcagca aacaatcatc gattgtccca
     3901 tttttcacag cttgagctac ttcttctaat gtcagagcat ccttctggga cataccggca
     3961 cttgctgttc gggtcagatc agacatgtgc gcagggtaac caagtttctt gcctgtatct
     4021 acagctaatg tgcggacata ggttcctttc ccgcattcta cttcgaagcg ccaagaaatg
     4081 gttcctgtca cttcgtctct tgtcaattca cttgtccgga taaaacggta aatctgagcc
     4141 tttctaacag gacgctccac tgtttcatta ttgcgggcat attcatataa ccgcttaccg
     4201 ttgactttta ccgctgagta cattggtggt atctgggtga tctcaccaat aaatgaagcc
     4261 atcacttcat ctattttttt ttcagataaa gacatatcta ctggttttgt ttctacccgt
     4321 tctccggatc gatcttccgt cgttgtactg aaacctagag ttatctcgcc tacatagatt
     4381 ttcccagaat ctgtcaaata ttcgataact tttgtggctt ttccaataca aatgggcaac
     4441 acaccgtcta cgtctgggtc aagcgtaccc ccatgtccga tttttttcgt atgtaagatt
     4501 tttcttaatt tgaatacgca atcatgactt gtcattccgc gttctttcca taaaggtaat
     4561 aacccgtcca tgtcttttct ccatttcatt tttgatttaa ctgaattatt ataacacatt
     4621 gcatcaatta ctgacgaatt tgacatcata atgaacggtt tttatttttc ggcaataaaa
     4681 acgtaacaaa attcatgctc acgtaaatag aaatcatgtt atgctttttt aaaggaggga
     4741 atagaatgga agattttttc tttgcaccat ttgattggat ttatgggtgg cttacaggta
     4801 aatagtttgg ttttcacttc ccaaaaacga aaaaaaagac tggccattct tccagtcttt
     4861 tttccgtttt ttttatttat tttttcagtt tcttaatcaa tgtaaaaaat tctattttgc
     4921 gggatcgatt ttatcatttt attggtcaag atttttccat ttccaatcca atacttctgg
     4981 cagatcaata ccattttcac gaatatagtt ttgatgttct tgtaatttct gatccatttt
     5041 tttcgcaaaa tcttctgctt gacttccttt gatttttaaa gctgcgtctt ttgccaaatg
     5101 ataacgatca agttcattta ctacacgcat gtcaaatggt gtcgtgatat ctccattttc
     5161 tttgtagcca tgaatcatta aatgatggtt atgacgatca aagaaaatcg tgcggaccaa
     5221 tgtttcataa ccgtggaaag caaataagat cggtttgtct tttgtaaaat attgatcaaa
     5281 ttcttcatcg ctcaacccac gcggatcttg acttggatga cgtaatttca ataaatctac
     5341 gacattgatg aaccgaattt ttagttttgg gaattcttcc agcagcaaag ttacagctgc
     5401 taatgcttct aagttagatt ccgttcctgc agcagcaatg accaattcag gttcctcctc
     5461 ttttgctgtc gaagcccaat caatgatttt caagccttca ttcactaaga cggctgcttc
     5521 ttctgctgag taaaattgag cacgcggatg tttggaagag atgatcaaat tgattttttc
     5581 tgacgagcgg aatgctttat ccatgactgc gagcaatgta tttgcatccg caggcagata
     5641 ttcacggatg aactccgcct tcttttctgc caaatgtgtc aatattcctg gatcttggtg
     5701 gctatatcca ttgtgatctt gctggaagac tgttgagctt gcaatgagat ttagcgaagg
     5761 ataatcattt cgccaagaaa ggtcatgtga tttccgcatc catttgaagt gctgagtcaa
     5821 catagagtca accactcgca agaatgattc gtaacttgca aaaaaccatg ccgtccagtc
     5881 agaacgtacc cttctaagaa cccttcatct tggtgttccg ataattgact gtcgatgacg
     5941 cgaccagatg gcgaaagcca ttcatcattt tctggttcga ttttttccat ccattggcga
     6001 tttgtcgttt tgaaaacttg attcaatcgg tttgattttg tttcatctgg tccaaatata
     6061 cggaaatttt ctggattttt ctcaatgatc tcgcgaacga atttaccaag ttcggtcata
     6121 tcttctgctt taacggagcc tggtttttca aattgcaaag tatatttttt ccaatctggc
     6181 ataataagcg gtcttggatc gattccacca ttggtgatag gattttttgc cattctttgg
     6241 tcaccagtag gcgcgatttc tctgatttct tctaaaatac gtccttgtgc atcaaataat
     6301 ttttctggtt cataggattt cagccaacgg agtaaagcat ctgcatgatc catatgttct
     6361 tgatctacag gaatcggtac ttggtgtgct cggaaggttc cttcaattgg ttccccgtcc
     6421 cattcttttg gaccggtcca tccttttggt gtgcgtacga tcaatactgg ccattttggc
     6481 atcacgacat ctgttgctgt cttcaggcga gcttcttttt ggatggattt aattttctcg
     6541 attgcttgat ccattttttc agccataaca ggatttaatt tttctgaatc atttccttca
     6601 ataaagaaag gttcccagcc aagtccgtta aagtaatttg ctaattctcc atctgattta
     6661 cgtgccaaga tcgttgggtt ggctattttt gcaccattta aatgaaggat cggtaaaaca
     6721 gctccatcta tcactggatt gataaagaca tttgagaacc agcttccagc taaaggaccg
     6781 gtttctgctt caccatcacc aatcacgaca gcagagatta cttcaggatt gtctaataca
     6841 gctccgactg catgtgaaag agagtatcca agctctccgc cttcatggat agaacctggt
     6901 gtttctggtg ctgcatgaga agcaatgcct ccaggaaatg aaaatcgttt gaataatttt
     6961 tgcattcctg tctcatcttc cgttacttct ggatagatct ctgtgtaact tccatctaaa
     7021 taagcattcg ataccatgac ttgtccgccg tgtcctggcc cttcaatata aaacatgttc
     7081 aaatcatatt tattgatgac tcggttcaaa tggacataaa taaaattttg gccaggaatc
     7141 gttccccaat gaccgattgg atgttttttg acatcttcgg gtttcagtgt acgtctcagt
     7201 aaggggttat ctttaagata aagttgccca acagaaagat aattagcagc acgccaccaa
     7261 gccgtcattt tatcaaagta ttctttacta gaataatcca tacataacac tccttttcat
     7321 tattactctt attctagcgc agatactccc aattgaaacc taataagcac ttctgttcat
     7381 ttgtttggat tttatatcta gccaaatgaa caggtataaa aaaccacgaa atttttcctt
     7441 tcgctgacgt tttttacatt gatccaaaaa acttgtcgtt atcacaatgg ctgttccgca
     7501 gttcatttcg ctcctcatca tgcataattt gctgaatgac gctgggccag ttaacacatt
     7561 gctggaaaac tggggctgga tcgagcaatt cctttcctga ttgatccgtt ttatgtaaaa
     7621 gtctctgtta tctttgtcaa tatgtgaatt ggtatcccag ctactatgct aatttctaca
     7681 gggattatcc aaaatttgga tcgtgaccaa atcgaagcgg cgtaatcgga aatatcaata
     7741 acttcaacgt gatttttctt ttaactgtca acggaccgac aaactcaaac ttttatggtg
     7801 caggttccac tgatcttcta gttacttggc tatacctgac tgtcactaaa atagattaca
     7861 atctagcttc cgttatcggt atcttgatct ttatctatat gcaaaaattt atatactact
     7921 agaaaaaaga aagtcgaatt ttttactttt ctttttctct tttttggtta gctgacacgc
     7981 cgcaagacta aagtcatgga actcttaggt aatacgtctt aatgaaaaca aaaaaatccc
     8041 gcaacccctg atttcacagg atttgcggga gtttttgatt agtctttgtt caagtcgcga
     8101 agcaattcat cgatatggtt gccatagaca actgattcat caagttcaaa gaacaattca
     8161 ggtgtcttat aaatacttag attgtgccct aactctcgac gaatcaatcc tgatgcttta
     8221 ttcaatccat cttgtgctgt tttcttatct gatgctaagt cagataaaat gctgtaatag
     8281 atcgttgctt gctgtaagtc ccccgtaact ttcacatctg tgatcgttac gttctccaca
     8341 cgagggtcac ggaccttttt gcgcaaaata tcattgactt cttttaagat ttcttgaccg
     8401 actcggcggt cacggtagtt agccataatg attggcctcc ttttatcagt ttttagtcat
     8461 ttttgacttc ttccatgata aatccttcaa ttacgtcatc tactttgatg tcattgaatt
     8521 tttcaatcat tgcaccacat tcataaccca ttttgacttc tttcacgtca tctttgaatc
     8581 gtttcagact tgcaagttgg ccttcataga ttacgattcc gtcacggatt acacggacac
     8641 cgctatctct acgaatatat ccttctgtta cataggcacc tgcaatcgta ccaactttag
     8701 aaactttgaa ggtttcacga acgatcattt gaccggtgat tttttcttca aattcagggt
     8761 ctagcatccc tttcatcgca gtttcgattt cttcgatggc tttatagatg atacggtgaa
     8821 gacgaatatc gacttcttca gtatcagctt gcacttttgc ctgcggtgta ggacgaacgt
     8881 tgaaaccgat gataatagcg ttgctggctg ctgctaaggt tacatcactt tcattgattg
     8941 ctccgacagc tgaatggacg atttttacac ggacaccttc cacatcaatc ttcttcaatg
     9001 atgcagccag tgcttctgct gaaccttgta catctgcttt gatgattacg ttaacttctt
     9061 tcaattcgcc ttctttcagg ctttcaaata agttatccaa agtcacacgg cttgtggcag
     9121 cacggttttc taataatgca cgtttcgcgc gttcttcacc cgctgcacgt gctgtctttt
     9181 cgtcttcgaa gactacgaaa cggtcacctg cttgaggtac atcattcaag ccagtgattt
     9241 ctactggcgt tgcaggtcct gcttctttat cgcgacggcc aagatcgttt gtcattacac
     9301 ggacacgtcc gtaagtatta ccgacaacga ttgggtcacc aacatgaagt gttccttctt
     9361 ggacaagtaa ggttgacaca ggacctttac ctttatcaag acgtgcttcg atcactgttc
     9421 cgattgcgcg ttgtgttgga tctgctttta aatcttctac ttctgcgact aacaggatca
     9481 tttccagcaa ttcttcgatg ttttggccga atttcgctga gatctcaaca aagatcgttt
     9541 ctccacccca tgcttcagga attaactcat actcacttaa ttcttgcata acatgttgag
     9601 ggtttgcacc tggtttatca attttattta cagcgacgat gattggaacg cctgcagctt
     9661 tggcatggtt gattgcttca actgtttgtg gcatgacgcc atcatctgca gcaacaacta
     9721 agatcgtgat atctgtgata cttgctccac gtgcacgcat acttgtaaag gccgcatgtc
     9781 ctggtgtatc caagaaagtg attggtttac catcaatatc gatctggtag gcaccgatat
     9841 gctgtgtgat tccgccggct tcgccgcttg ttacacgtga atggcgtaat gtatccaata
     9901 aagttgtttt cccatggtct acgtgtccca taatcgttac gactggcgga cgtgagacta
     9961 atttatctgg atcgacttct tctgcctcga agaatttatc gatatcagca atgtcaactt
    10021 ggactttttc ttgtggttcc atgccatagt ccgtcgctaa cagttcaatc gtatctttat
    10081 ccagtgcttg gttttgattg accatgacac caagcataaa taatttttta atgatttctg
    10141 ctggctcacg atgaattttt ttcgcaatat ctgctacgtt cataccttca gtatactcta
    10201 atacatctgg caattcacgg aatttgcgtg gtggtacagc tggtttagtt gattgttgct
    10261 gtttgccttt tttacctttt ttattataac gattacggtt attattatta aatttgccgc
    10321 gattgttatt gtttggtttg ttgttattgt tagtacggtt ttgattgccg ccttggctcg
    10381 ttccttgacg attttggctg ctggtatttc ccgtattatt ttggtttgtt gttggttttt
    10441 ttccttgatt cacttgaccg ctcccttgac cgcgatcttg ataactgcgg ttatttttat
    10501 ttttttgttg attgttcgtt gtttcttttt tacgttgatc gttcggctgt tgattggctg
    10561 gttttctgct tgcttgttgt gcagcttgtt gtttcgtgct tgggtttttg aacgcttgtt
    10621 gcaatttctt ctcatcattg ctagtaatcg cacccatatg gtttttcaca tcgatgccaa
    10681 gttgttgtgc tttttccacg acgtctttac tagatttttt gagttctttt gctaattcat
    10741 agattctttt gttgcccatg caatcacctt cctaaccttc aattagctcc ttggtcttct
    10801 tcgcaaatcc ggcatccatt attccgaata ccttgcgagg ttttccgatc atctgcgtga
    10861 tttctgcttc agaaaatagc tcaaagcaag gaacttcata atatgaactc ttatctttta
    10921 tcttttttct tgtattctcg cttgcatcgg ttgccacaaa caccaatttt gctttttgtt
    10981 tgcggatttc agcaatggtc agttcttcac ccgtgattag ttttcccgcg cgcatcgcta
    11041 gaccgatgag attcatggct ttttgccgat taactgtatt cattttccaa ataactcttt
    11101 acgtgctttt tggtgagtga catagtcaag aagttcttga taaaactcat ctgttaatgt
    11161 tgtttcgagt actcgatcga gaattttttt atcccaagct tgttgtactt cttccggctc
    11221 aagtgagaca tacgctcctc gtccaggcat ttttccagtt ggatcgatcg atacttcccc
    11281 ttcttttgaa cgggtgattc ggaccatctc ttttttaggt ttcatttcac ctgaaaccac
    11341 ggatttgcgt aaaggaattt tcctttgttt catctctttg cctccttact aaagttaagc
    11401 ttctggtttt tcttcgaccg tttcattgtt gaatgcaatt gtttcatatt catcgtctgt
    11461 taaatctgac tgaacgattg cttcatcatg cagttcttct gtttcttttt gttgttcttg
    11521 attttcataa aattccgtca tgtcagattc tgatttgata tcgattttat ggttagtcaa
    11581 tttagcagct aaacgtgcgt tttgtccgcg tttaccgatt gctagcgata attggtagtc
    11641 tggaacgaca actgtgcagg cctttggatt tgtctcatcg aagatcacat ccatgacttg
    11701 tgcaggattc aatgcatttg caatgaatac agctggatct tcattccatt cgacgatatc
    11761 catgttttcg ccttttaatt cattgacgat tgcttgaact cgttgtccct ttggcccgac
    11821 acatgtaccg actgcatcga tatttggatc atttgagcga actgccactt ttgaacgatc
    11881 gccagcttca cgggcgatgc tgacaatttc aacgattcca tcatagactt ctggtacttc
    11941 ttgctcaaat aagcggcgca ataggtctgg atggctgcgg ctaacaaata cttgcggacc
    12001 tttagacgta ttttctactc gggaaacata aactttgatt cgatcatgtg gttgataaaa
    12061 ttcatttggc atttgatctt gtttagaaag aactgcctca attttaccaa gattgacata
    12121 aatataacgt ttatcctggc gttcgacgat tccttgcatg atgtcttttt cataagcact
    12181 gaattcgtta taaataatcg ttctttctgc ttcacgtaca cgctgtaaaa taacttgttt
    12241 agccgtttgc gcagcgatcc gcccaaaatc ttttggtgtt acttcaaaac ggatcgtatc
    12301 accgatctca tatgctggat tgattaaaag tgcatctttc aatgagactt ccaactgtga
    12361 atccatcact tcttccgtta cttcttttac cgcatagaca tggatctttc cttttttctg
    12421 atcaaattcc acttcgacgt tttgtgcttg gccatagtga cgtttgtatg cagaaaccaa
    12481 tgctgcttct aaagcatcga tcacgatttc ttttgagatc cctttttcag cttctaaagc
    12541 gtctaacgca tttaacattt ctttactcat tttttctgat tcctctccgt gattaaaatt
    12601 ggatagctaa gcgagctttt gcaatatttt ttcggtcaaa agtcaattct ttttctcgtg
    12661 tttttatgcg tattttcaat gtaagctgtt cagcatcaaa tgattgaaga aatccttcat
    12721 attgtttttc gccatctact ggttggtata aggagacatg gacataatca ccaagtgcct
    12781 tttcatagtc gctttctttt ttcaatggcc gctctgctcc cggggaggat acctccagga
    12841 aataagcttg aggaatcgga tctggatctg ctgcatcaag tttttcactc agtttctcac
    12901 tgacaaaggc gcattcttcg atatcgatac cgccttcttt gtcaataaat actcgtaaga
    12961 accagctttt tccttctttt acaaattcga cttcgacaag ttcaaaattt tgttcatcta
    13021 aaatcggtgt caccatttct gtgactgttt caacgacgct actcaaaagt ttcgcctcct
    13081 taaaaaatta agtgcgtaat tccattaaaa agagtgagcg aaaaatccgc tcactcacag
    13141 ttagtatcat tacctataag aactatacca tactctgctt aaaattacaa gcagacagga
    13201 aaacagatgc ttatagcata tcgaataacg ataattgatt ttcatctggt agatctttca
    13261 atacgccatt gtcgttcata tattcgatca atgtttttga aactttaccg cgggtggcaa
    13321 gatcttcttt ggataaaaat ggaccgtctt ttcttgcttc aacgatttgt tttgccacgt
    13381 tggttcccaa acttgggaca gctctaaatg gtgcgatcaa cgtatcacct tcaataacaa
    13441 agttaacggc atccgattta tacaaatcaa tcattccaaa tttgaatcct cgttccagca
    13501 tttcattggc taactccagc actgtcagtt gattcttttc ttttacggaa gcatctaatc
    13561 ctttgtccgt gatttctttc attgcttgtt tcacagcatc ttttcctttg cacatggaga
    13621 ctagatcaaa gtcgtctgca cgtacagaaa agtatgcaca gtaatacaaa atagggaaat
    13681 aaaccttgaa ataagcgaca cgaagagcca tcaatacgta agctgcagca tgggctttcg
    13741 gaaacatata tttgattttt gaacaagaat cgatatacca gtcgggcaca ttattttctt
    13801 tcatcgccga tagataggtt tctcgcaatt catcagggat cttgttccat tgtcctttac
    13861 gaacagtttc catgatttta aatgccatcc cgctatctaa tccagcatgg atcaaataaa
    13921 ccataatatc atcacgacag ccgattactt ccgccagtgt cgcatctcct cgtcgaatca
    13981 attcttccgc atttcctaac catacgtctg taccatgaga tagtccggaa atttgcagta
    14041 gttctgcaaa agttgtagga tgcgtttctt ctagcatccc tcgaacaaaa cgagtgccga
    14101 attctggaat acctaatgtc cctgttttgg aataaatttg ttcctgactt actccaagta
    14161 cttcaggacc ggcaaatatc cgcatcactt ccgggtcatc cgtagggatc gtttgtggat
    14221 cgatccctga taaatcttga agcatccgaa taacagttgg gtcatcatgc cctagaatat
    14281 ctaatttcaa tacattatcg tgaatcgagt gaaaatcaaa atgagtcgtt ttccattccg
    14341 aattttgatc gtctgctgga tattggatcg gcgtaaaatc atagacatcc atataatcgg
    14401 gaataacgat gatcccccct ggatgctggc cggttgtccg tttgactcca gtagcgcctt
    14461 ttgctaatct gtcgacttct gcacttcgaa actgcagatt attgtccctt tcgtatcctt
    14521 ttacatagcc atatgctgtt ttgtcggcga ctgtaccaat cgttccggca cggtaaacat
    14581 agtcttcgcc aaacaatact ttcgtatagt tatgggcttc tgcttgataa tcacctgaga
    14641 agttcaaatc aatatcgggc actttgtctc catggaatcc taaaaatgtc tcaaatggaa
    14701 tatcatgacc gtctttgttt aagcgtgcac cacattttgg acattgtttt tctggcatgt
    14761 caaatccaga accatacgta ccatcttcaa aaaactcgga atattggcac tctggacaat
    14821 aataatgagg ggctaacgga ttaacttcag tgattcctgt catcgtcgca acaaagctag
    14881 agccaacaga gccacgtgaa cctactagat agccatcttc attactttta tgcactagtt
    14941 tttgggcgat cagataaata accgagaagc cattaccatt aatagaattc aactcttttt
    15001 ttaatcgttt ttgaatgatc tcaggtaacg gatctccgta catttgtttt gcttttgtgt
    15061 aactcaattc tgatatttca tcttctgaac ctggaatctt cggtgtataa agttcatctt
    15121 tgactggaat cacttcttcg cagatatctg cgattttatt cgtattttct actacgatct
    15181 ctttcgctgt ttcttcccct aaaaagtgaa atgcagttag catctcatcg gttgttcgga
    15241 aatggacatc cggcaaacta tgacgattca acggattcgc tccgcccata gaattgatca
    15301 aaatttttcg gtaaatggca tcttcttcgt tcagataatg tacattacct gttgcaacaa
    15361 cgattttatc caaacttttt ccgatttcta ccaaattttg gataatttct tccaaatcat
    15421 gttcattttt taccaattct tgctcgatca aaggcgcata gaccgctttt ggcataactt
    15481 caatataatc gtagaatttt gctcggtttc ttgcctcttc tacccctttt tgcatcatgg
    15541 cttcgaatat ttctccctta tcacaagctg agccgatcaa tagattttca cgcatctttt
    15601 tcaattggga gcgtggtatt cgtgggacgc gttcgaagta ttcgacattc gacattgaaa
    15661 tcagtttgaa caaatctttc agtcctgcct gattttttgc taaaatcgtt acatggaaag
    15721 gtcgagccct tttgtaagaa tctccttctc cgatatgctc atttagctga tcatgataaa
    15781 gcatgttatg attgtccatt gcctctttca caaagatcca tgctaagtga ccagttgctt
    15841 cagcatcata gattgctcgg tgatgctgtt ccaagctgac accaaatttt ttagataaca
    15901 cacctaatcc gaaacgtttg aattgaggat acagatatct agctaattcc aacgtatcaa
    15961 tgactggatt ggccgcttca ggaataccat accttgcgta gctagtattc aagaacccca
    16021 tatcaaaagc cgcgttgtgg gctaccaaaa ttgtatcttt tgaaaattca aggaacatcc
    16081 gcaatacttc ttcttccgac tttgatcctc gaaccattcc atccgtgata ccagtcagat
    16141 ctaccgttgt tcgtgacaaa ggatgtcctg gatcgataaa ttcatcaaag ctttcgatca
    16201 catttccttt atacattttg actgcggcta gctcgatgat cgtgtcatat actgcagaca
    16261 atcctgtcgt ttccacgtca aatacgacat atgtggcttc actcaatgct tcatgtgcat
    16321 cattataagc aattgggaca ccatcatcta cgacattcgc ttccacgccg tataagattt
    16381 tcacacctgc tttttttcca gcactatgtg cttccggaaa ggcttgcgca ccaccgtggt
    16441 ctgtaatggc gatagctcgg tgtccccatt tccctgcttg tgccacaaga tcagagatgc
    16501 tgtttgttgc atccatcgta ctcatattgc tatgaacatg aagctctact cgtttttccc
    16561 cttcaggagc ataatctttg cgaggtgtat gtttgacttc tatgatatct tgcgcattca
    16621 tgaccaaatc acggacaaac gtatcttctt gaatacttcc tcgtaccttt aaccagctac
    16681 cgacactgat cgcatcaaat acttgttcat ctttttcgcc gttcgagaat tttttgacga
    16741 taaatgatga agtataatct gtgattttca aagtcaagat cttgcgtttt gaacgtagtt
    16801 ctctgacttc tttgtcgaat acgtacccct caagtgtgac tcttcgttct tcttcaacga
    16861 tactgatcat tggtgtcgtt ggttcatcgg ctggaatatt tcgtcctagc tggatgtgga
    16921 tgggtccttc aagagctggc gattgctctt ttctttcctt tttcttttgt tcgtggacaa
    16981 ctagactttc agctgcttgc ttcataaagg cttctgcctg ttcttgtttg cgttcttcaa
    17041 aaagtttcaa tactcgttcc gcttgctgct catccacttc tggttcgata cgaaattttg
    17101 ggaatccgta actgacgaac aactcttcta ctaatggcaa gtattgttgc ttcaaataag
    17161 aaatagctcc tgtactgtcg attggcaaga tgactttacg atcttttatc acaggtacct
    17221 gcgttttcaa tacttgctgt gctagtggtg tatcgcattg ctgattttcc aaagcttggg
    17281 cccagtaatc ttgcaagagc tgttcatcga atgtctggtc atctgctaag atattcagtt
    17341 tgacttgtgc aatatcttta aaagcgatag tcagatgctg agtcaatgac cgatatagca
    17401 taataggcaa aattttggaa aaatgcagcg taaattccca taaacgcgat ttacgatgga
    17461 ccactacact atccatttca ccagcagtaa gcagtggatg atttttttct gcttcctcta
    17521 gttggatttg ttccaacagt ttttcaaata aatctcgttt ttctgacaaa gatatacgcc
    17581 ctttctgatg agaaacaatg caattgatcg catcttatcc tcttcttttt cagtaccttt
    17641 tagtatactc gttttttctg agttttacat taaaaaattg gaactgcgat tcaaatcgca
    17701 gttccaattt taaaagcgaa tcatcagatt gtcaaaaaca gctgattcag ctatttttat
    17761 tttctgtgtt ttaacgctta ttattcgcct tggttcaaga ggattggtaa agtattcacc
    17821 aattcttctt tacgtacttc aaccatttcg ccagttttct tgattttcac ttctacgatt
    17881 ccatccactg cttttttacc gacagtgatt ctgattggac agccaatcaa atcagaatcc
    17941 gcgaatttca ctcctgcgcg ttcatttctg tcatcaacca gtacttcata gccagctttt
    18001 gtcatcgctt cttctagttc attcgtcagg tttgtttggt attcgtcttt tagattcatt
    18061 tggacgatat gcaaatcaaa tggcgcaatt ccttttggcc agttgattcc ttgttcatct
    18121 gaatgttgtt ctacgattgc tgataataaa cgacttacac cgataccata acagcccata
    18181 atcacaaatt tttctcgtcc attttcgtct agtacagttg ctcccataga ttcactgtaa
    18241 cgagtgccga ttttgaagat atgtccgatt tcgattcctc gagtaaaggc aagaacacca
    18301 ttgttgtctg gtgaaggatc tccttcttgt acaaaacgaa gatcttcata agaaagtaca
    18361 gtgaaatcgc gttctggatt cacattttta tagtggaatc ctgtttcgtt cgctccagta
    18421 atggcattcg ctaaatcttg gacataacgg tctgcatata cttttacgtc atctgaaaca
    18481 ccgactggtc cgacagaacc aaactcagca cctagatatt tttgtgcttc ttcttctgtt
    18541 gcttcttcta aaaagtcagc agataaaaag tttttcagtt ttacgtcatt gacttcatgg
    18601 tctccgcgta ccaaaacaag gacaggttgt tcatccgcaa tgaacaatac agacttgatg
    18661 attttttgag gttcaacaga aaagaaatct gctacttcgt caattgattt tacgttaggt
    18721 gtagctactt tctccaattc aagctgtgtt tcatgcgatt tttttgatac atagtagctt
    18781 gtagccatct ccaagtttgc agcataatca ctttcagtcg aataacagat cgtatcctcc
    18841 ccgatttctg agatcgccat gaattctttt gagtctttcc cgcccattgc gccaccgtct
    18901 ccgataatcg agcgaaattc taagccacaa cgcttgaata cttcggtgta ggctttttcg
    18961 taatctttat atgtctcgtc caaactttct tccgttgcat ggaaagaata cgcatctttc
    19021 atgataaact ctcttccgcg aagcaaaccg aagcgaggtc gtttttcgtc acgatattta
    19081 ggttggattt gataaagatt taaaggtaat ttcttatatg aattgatttc attacgcatc
    19141 aaatcagtaa aagtttcttc gtgagttggt ccaaggatca tttttcgatc attgcggtct
    19201 ttgaaacgat acaagtttgg tccatatgtt tcgtaacgcc cggattcttc ccaaagttct
    19261 gctggcaaga tggcaggcat cagcatttct accgctccga ttttttcaaa ttcttcccgc
    19321 ataatgcgtt tcagtttttc caatacacga tttgctaatg gcagatatga ataaataccc
    19381 gctgatactt ggcggatata tccagctctt agcaagattt gatggcttaa tacttctgca
    19441 tcatttggaa cttcgcgtaa tgttggaatc aacatttttg attgtctcat tctatatact
    19501 cctttatttc ctcaaaatag ttatcgtttg ttattatacc ctatttgtca actttttcta
    19561 gaaaaagaat cgttgaatat cgttccatgt aactagcacc atcaacaaca ttaaaaatcc
    19621 aaagccagct aatgtcaata ttccttcttt ttcttgactt aatggtttcc cgcgaatccc
    19681 ttcaaaaata ttcaagacta atttcccgcc atctaaggca ggaatcggaa gcagattgac
    19741 gattccgaga ttcattgaaa gaagcgccat caaactaata acagttgtta caccttgatt
    19801 tgctgcttct gaagacaatt ggtacatcat gacaggtccg cctaatttat ctaaactgaa
    19861 acctgtaaat agtgatccta aggctttgaa aatttccaat gaaccgctga aagcttgacg
    19921 cgtccctcca atgatcttat ccataaaacc tgtattcatt ggggctttta taccaagttg
    19981 gccaactttt tcgccatttg actctacaga tttcggtgtc acgtcgacag attgcacctg
    20041 accttctcgt tcgatcttaa agtctaatgt tttatccgga tttttcgtaa tgactgttgt
    20101 cagatcattc catgaatgga tttcttttcc gtccacactg actacctcat cattttcttt
    20161 taagcctgct tctgccgcag ctccattagg catgatctct cctacgcgat tagtgttcgt
    20221 cacttgaaca ccaccttgca taaatgccag aatgatgaat aacaggattg ccaaaataaa
    20281 gttattcatg ggtccagcaa agttggtcag catacgctgc cacaatttag ccgattggaa
    20341 ctgtacgtct tttggcgcaa ttctgatttc tgttccgtcg ttttcaataa tcgtcgcatc
    20401 gtgatcaaca gggtaggtta ctacttctgt ttcgtctcca ttcacataac cagtgatagt
    20461 cagctcatct tctaaatcat aacggctcaa ttccatcggg atagcatttg tcaattggat
    20521 tttcttactt aaatttatct tctctacgat gccgtcagaa tttagtaaaa gagaaagagg
    20581 cattcctggt gccatttctg tttcatcatc cccattgcct gccattcgaa cataaccgcc
    20641 aataggtaat aggcgaagcg tatatgttgt cccgtctttt gcttgatgcc cataaatttt
    20701 cggacccata ccgatagcaa attcgcgaac gaggattcct gatcgtttcg caaagaagaa
    20761 atgaccaaac tcatgaacaa tcactaatat accaaaaacg atgataaatg tcagaatcgt
    20821 tttcatagct tttttctcct ttacttgttt tttgtgtttt ttataaaaca acgataaaac
    20881 actataattt taccatagta taccaaagac accaatagta tcaatataat gaaaaataaa
    20941 aacctgcgtc ttttatgaaa ttcacaaaga aacgcaggta tacttactaa ataatgccaa
    21001 ataaatgcat gataggaaat acaaacagca aactgtcgaa acggtctaag attccaccat
    21061 ggcctggtaa gatatttccc gagtctttca catcataatg acgtttgatt gcagattcga
    21121 ccaaatcacc aaattgtccg actgctgaaa aaataatcgt catcagaagc atgacaaaca
    21181 taccatgatt aaatagttct tttgctgggt atagcaataa ataaagaaat gcgactacaa
    21241 cagcactacc aataccaccg agcgcacctt caatcgtttt gttgggcgaa atatcaggac
    21301 ttaacttatg ctgaccataa cgtcgaccaa ccatatatgc tccgatatca gttgcccaaa
    21361 caataaatag accaaatatc aaaacagcca agctttctgt tcgtgcattg acaaaattct
    21421 ggaaacctat tcccacatac aagctgacaa gcacaggaaa acctgcttca tcaatcgtat
    21481 acgtattttt agaaataact gatgttccta aaagaatcat aactataaga taaaataaca
    21541 taaatgtact tacattctct ggaagaaaga aaaaccatct ttcttttggc aaaacaagta
    21601 acactgcacc aacggcggat aaaactcctt caaaacttaa gagtgtcaat cctttcatcc
    21661 gaaacagctc gtaaactcct attactgcta agattgctgc tgtaagctca accgctatgc
    21721 ctccgatcca tataatcgga ataaacaaga ctagcgccac ggctgctgtg accacacgtt
    21781 gtttcatgac tgatcccctt tctcatcttc tgtttttttt acaccgccga atcgacggtc
    21841 tcgattttga taagaggcga ttgcttcttc aagatgcgca ccatcaaaat caggccaaag
    21901 cgcttttgta aaatacagct cactatatgc gatctgccat aacagaaaat tactaattcg
    21961 ctcttctcca cttgtacgga tcatcagctc aggatcttgt aactctttag gcaagaaacc
    22021 tgtcattaaa tgcttagcaa tcaattcatc atcgatttct tctgcgtgta tttctccttt
    22081 tgctacttct tcggcaattt cttggacagc agtcacgatc tctgctctgc ttccataatt
    22141 caaagcaaaa ttcaatacca ttccagtatt gttttttgtt tgctcaattg cacgtcttac
    22201 agcatcttgc gtatgttctg gcaatacgtt ttcataaccc atgacatgaa ctttgacatt
    22261 ttctttaatc aattctggta cgaacgtatc aaagaagtcg acaggtagtt gcatgagaaa
    22321 acttacttca tcttttggtc gcttccagtt ttcagtcgaa aaggcataaa gcgtcaacac
    22381 tttcactcca agccttgatg ctttttttgt gactttcttt actgtttcca tgccttcttt
    22441 atggccagca actcgtggca aacgacggtt ttgcgcccaa cgaccattgc cgtccatgat
    22501 gatggcaatg tgctgaggaa ttggtccatc ggtattaaat tgaaattcgc ttttttcttg
    22561 aatatatttg tttttttgcg gaaaaaaacg taacattctt tttcctccta tacttaaaag
    22621 attcccacta tcttaagtat tatagcaata tctaactgtt tttcctatga aatcattgtt
    22681 tttttattta aaaactgaat aattttttct ttttttaatt tttaaataaa agaaatataa
    22741 ataaaaacag aggaagcgga atgatccgcc cctctgtttt tatgatttta ttaaacttcc
    22801 aataattctt tttctttatc agcagcgatc ttatctactt ctttgatgct gttatctgtc
    22861 aatgtttgta cgtctttttc taatccgcgc aaatcatctt cggtaatatc accatttttt
    22921 tgttgttttt tatattcatc gatcgcatca cgacggatgt tacgaatcgc gatttttgca
    22981 ttttctgctt cttttttaac atctttagct aattcttttc tgcgttcttc tgtcagttgc
    23041 gggataacta agcgaatgac atttccatca ttggttggac tgattccgat atcacttgcc
    23101 ataatagctt tttcaacatc ttgaagaatg ctcttgtcaa aaggtgtgat cattaaaaca
    23161 cgtgcttctg gaatttggat tgatgccatt tggttcaatg gtgttggcgc accgtagtag
    23221 ttcacagtaa tacggtccaa aaggctagcg ttcgcacggc ctgcgcggat ttgcccaagt
    23281 tcacgttgaa gattttgtgc tgcttttttc attttttctt ttgcttctgt cataattgca
    23341 tctgccattt ttatttcccc cttacagttg ttccgatatt ttcgcctaag attgcacggc
    23401 gaatatttcc gtgttcattt aaattgaaga caagcagtgg aatatcatta tccatgctta
    23461 gtgaacttgc agttgaatcc atgacttgta atcctttggc aataacttcc agatgagtca
    23521 gttcttcaaa ttttactgca tttgcatcta ctttcggatc agcagaataa acaccgtcta
    23581 cattgttttt agccatcagg ataacatctg cgccgatttc tgccgcgcgt aatgctgctg
    23641 ttgtgtctgt cgagaagtac gggttgccgg taccgcctgc aaagataacg atccgtcctt
    23701 tttctaaatg acgttctgct ttacggcgaa tatatggctc tgcgatttga cgcatttcaa
    23761 tcgaagtttg cacgcgtgtc ggtacaccca cgttttccaa cgtatcctgt aaagctaagg
    23821 cattcataac tgtagctaac atacccatgt aatcagcttg agcacgttcc atgcccattt
    23881 gtgctccgat ttgtccgcgc cagatatttc cgccgcccac aacgattgcc atttcaacgc
    23941 ccagctcgtg aacttctttg atttcttgga tgatttcttt gattactggt ggtttgattc
    24001 caaatcctgc gtccccagct aaagcttctc cactcaattt taagactaca cgctgatatt
    24061 tcggtgtgac catgttcatt cctccaatat tttctattgc cattttacca tacttagatg
    24121 atttcgacac tctaagactg gtttttcttg ttttgcttaa aaaaagggag cgcacaaacc
    24181 gtgcgttccc gtctgttatt caacagaatt attttttcac ttggctcata acttcttcta
    24241 caaagttatc ttcgcgtttt tcgatacctt cgccaacttc gaaacgtacg aatgttttaa
    24301 cagttgcgcc ttttgatgca acgtattttt caacagtcat atcaggatct ttaacgaatg
    24361 gttggtccac caaagcgatt tctgctttga atttcttcaa gcgtccttct accatttttt
    24421 caacgatatt cgctggtttg ccttcgttca atgcttgttc tgtaagaaca gttttttcat
    24481 gttctaattc agcttctgga atttgtgatt cgtttacata acgaggattg atagctgcta
    24541 cgtgcatagc tacgtctctt gcaactgttt cgtcagttgt tccatctaga acagtcaata
    24601 cggcaatacg tccgcccata tgcaagtatc caccaaatgc agcattgtcg tctttttcta
    24661 caacttcaaa gcgacggaag ctgatttttt ctccgataac ttgagttgct tcgatcaagt
    24721 cagattcaat tgtaccttta tctgttttga ttttcattgc agcttccatg tctgctggtt
    24781 tgttttcagc aacaagttct gcaatttctt ttactaaatc ttggaacatt tcgtttttag
    24841 aaacaaagtc tgtttctgag tttacttcaa cgattgctgc agtgttacct ttcacagcaa
    24901 ctgaagccaa tccttcagct gcaatgcggt catttttctt cgcagccttt gccattcctt
    24961 tttcacgtaa aagatcaact gctttttcca tgtcgccttc tacttcaact aatgcttttt
    25021 tcgcatccat cattcctacg ccagtcattt cgcgtagttc ttttaccatt ttagctgtaa
    25081 cgtctgccat gtttaactcc tcctgtgtgt ttttcttttt aaaaaaagct gcttcaaagg
    25141 agagtgaggc ttgtcgccca gcctttgaaa cagctccatc attcaaaatt attctgctga
    25201 agaattgtcg ccttcaacaa catctacgat ttcttcgatt gatgttgctg tgttttcttc
    25261 tgtgaaatct tcttcaacta cttgatcttc accttggttt ccttcgatga aagcatcagc
    25321 catttttgaa gtaatcaatt taacggcacg aattgcatcg tcgtttgaag ggattactac
    25381 gtcgatttca tctggatcgc agtttgtatc aaccatagct acgattggga tatttaattt
    25441 gtgtgcttct tgaacagcaa tacgttcttt acgtgggtca acgatgtaca ttacatctgg
    25501 gattcttggc atatcagcga taccgcctaa gaatttttca agacgttcac gttctttgtt
    25561 caaaccagca acttcttttt taggtagaac ttcaaaagtt ccatcttctt ccattgcatt
    25621 gatttgtttc aaacggctga tacgtttttg gatagtatcc cagtttgtca atgttccgcc
    25681 taaccaacga tggttaacga agtattgtcc tgcacgagtt gcttcttctt tgattgcttc
    25741 ttgtgcttgt ttttttgtac ctacgaataa tgctacgccg ccttcttcag caacgctctt
    25801 catgtaatca taagctgcat ctactaattt aactgttttt tgcaagtcaa tgatgtagat
    25861 tccgtttctt tctgtgaaga tatatttctt catttttgga ttccagcgac gagtttggtg
    25921 accaaagtgt acgccggctt ctagtaattg tttcatagaa attactgcca tgtttgtttc
    25981 ctccgatttg gttttatttt tcctcttctt atcttctacc ttgccggcaa actttttcaa
    26041 gcacctaccg acaatccaat aagaatgtgg atttgctgat gcataaagca ccctcagcta
    26101 ttatacagac aaaagatccg tatcgcaaga gcaaatatac ttttctgctc aacattcaat
    26161 agtttacctg aaagataaaa agaattcaag aaaaacagta gatttctctt tgtttcttta
    26221 ttatcctttt tccataaaat aattttaggt ataaaagacg aatattattg ggcaatcctt
    26281 cttttttttg ttatgataca tgtgaaatct ttcgtaaagg agaaagaaaa tgcgcaaagc
    26341 tcaaagacat ttgctgatta aacaagtcat tgaagaattc ccgatccgta cgcaagaaga
    26401 actattaaac aaacttgggc attatggcgt taccgccacg caggcaacga tttctcgaga
    26461 tatccgtgat ctaaaaatcg tgaaggcacc tgatgaaaat ggcgtttccc gcttcgtatt
    26521 gtttcgcgaa aaaggaactc ctgagactag gactgaggaa gaggatcggc tggttcaaat
    26581 gattgaagac attgtattga aagtcgaacg agtccacttt ttgacgatcg tcaatacctt
    26641 gccagacaat gctcatttat ttgcatctgt tttagatgag atcaaacctc cctctattgt
    26701 cagtacaatt gctggattcg atacgatcat ggtcatctcg gaaaatgagg aggacgcaaa
    26761 acgagtagaa gaatttcttc accatccaac tgaagtatca cttttttaat cagagcacgt
    26821 attcctaata tggaatgcgt gtttttttat tttcaatatt ttccttattg aataaacctt
    26881 tacactcttt acaaaaatac atcatttttt attcaaattt ttcaacacct tgattgaatc
    26941 acaataatca tcatttatat tggatgttaa caagagcagc ttcttgctct ttgtgtattt
    27001 caaaaaagaa atgaaaggaa atcatatgta taaattatta acttctggac cattgacgac
    27061 ttctgaaaca gtgaaaaatg ctatgctgat cgatcattgt acttgggatc aagaatacaa
    27121 agaaattaca caatggataa gaaaaaaatt attggatctt gctcaagtat cggaaggaat
    27181 atacacaacg gtcttaatgc aaggtagcag ctccttcggg gtagaatcgg tattaagttc
    27241 tgtgatcagg ccagaagata ctgtactgat ttgttcaaac ggcgcatata gacaacgaat
    27301 gatcaacatg tgcgaatgtc tgaaactgaa ctataccgtt tatgaggtat cggaacatca
    27361 aatcctccaa gctcatgtga ttacacagct gattgaagag aatccgtcta tctccgcact
    27421 agcaatggtc catagcgaga ctacttccgg tattttgaat aatttggaag agattgctgc
    27481 tgtcacacaa gaaagagatc ttttgtttat agtggacgcg atgtcaagct ttggtgggat
    27541 accgatttgt gtagccgaac tagggattga ttttttgatt tccagctcaa acaaatgtat
    27601 tcaaggtgtt ccaggctttt cttttgtact tgctaataaa gcggaactgg aaaaacgaaa
    27661 aaatgttagc cgaagtttgt cacttgatct atatgaccag taccgcgtga tggaaaacga
    27721 gaatggcaaa tggcgtttca cttcccctac ccatacggtt ttggcttttc aacaagcgct
    27781 gaaggaacta gaggcagaag gaggaatcga aactcgatat tcacgttatc tcgccaataa
    27841 tcatgtgcta cgacaaaaaa tgacagagat ggggttccag cctttcattc aagaatatca
    27901 ggggccattc atcacctctt ttctttaccc taaggactgg cgatttgatt ttaatcattt
    27961 ttatcagtat ttaaagcaaa atggatttgt gatctatccc gggaaaatca gcgatcatga
    28021 atgttttcgt atcggaaata ttggcgagat ctatccggaa gatattttgg aactgacagc
    28081 aattatcaaa gactataagg agaacgttaa tgattaaaac tgtaattttt gattgggcag
    28141 gaacaactgt ggactttggc tgtatggctc ctgtccacgc ttttagaaat gcttttctag
    28201 aaaaaggaat ccaactgact gacaaagaaa tccgctaacc gatgggaaaa ttaaaatggg
    28261 atcatatcca acagcttctt tccctccctt ctgtcaaaaa acaatggata gctatttatg
    28321 gacaactacc tcaggaaaaa gaggtggagg aattgtatca acaatttgaa cattatctgt
    28381 ttaaagattt ggtcaagcat tctaccttga aacccgacac attggaaacg atcgaacggt
    28441 tgagaaacgc tgggatcaat attggttcca cgaatggatt taatgctcct atgatgacaa
    28501 tcgtagcaga aaccgcaaaa aaagaaaggt attcaccaga cttcatggca acttttgaag
    28561 acgtcgaagg gtatggtcga ccgtttcctt atatgatcca taaaaacatg cagtacttca
    28621 aaaacaagtc ggttgatgaa gtaatcaaag tcggagatac ggtctcagat atccaggaag
    28681 ggaaaaatgc cggagtgttg acagtaggtg taattgaagg tagttcgcta atggggattt
    28741 cttatgatga gtaccaaaaa ttagagatgg ggaaaaagat cgtttaaaac agtctgttgc
    28801 tcttagattc caacaagccg gcgctgatat ttgtgtaaac aatttaaaag agttaacaga
    28861 atacattttg tacgctgaca tgaatccaga atgactttta tacaaaataa gaggttgtga
    28921 aaaagctgtc ctgtatcaag taataaaaac agccaagcaa aaatagctcc tcatattttt
    28981 cgtttggtta ggcttattac cgcagatgca agcttttgaa caccgtttat tcgaaaaaaa
    29041 aaagggagct gtgacacgac ttttgtcaca gtccccttat ttgcaaacaa acagtgttta
    29101 agagacaacc tctccactaa acggtgttca ttcagctgac tctcggtatt tgctcaaaaa
    29161 acgataaaat ttgaaaagct attttctctt ttgtttctct aatactcgga tcagaacgct
    29221 gatttcacat tctctttttc ttacatctta tctggtgcat gaagtcctag caaacgaagg
    29281 tcttctttca ataatacagt cactgcataa actagagcta agcgtgcttc tttttgttca
    29341 tcatcagcta aaatcttcgt atgagcatag tatttgttga atgcttgtgc taatttgatt
    29401 gcatgtttcg caatcactga cggttcgtat ttttccccag cagacaagac cgtttcaggg
    29461 tatttttgaa ctaatttaac cacttcccag ctggtatcgt cattcaatgc atagtctgct
    29521 gtttctgaag ggacaaatcc tgctttttcc aacaggctga ctgcacgagc atgtgtatat
    29581 tgcacataag gtccagtttc tccttcaaat cgaacaactt cttccaggtt aaagtcaaat
    29641 gtattcagtc gatcgttttt caaatcgtgg aaaatgactg ccccaacccc aacttgttta
    29701 gctacagcat ctttgttttc caaatcaggg tttttctcgc tgatttgttc tttagcagat
    29761 tggattgctt catttaatac ttcttctaat aagacgatct ttcctttacg agttgataat
    29821 tttttaccac cttgcgtgat caaaccaaat ggaatatggt gcatgtcgtc agaccagtca
    29881 aagcccattt ccttcaagac agctttcaac tgtttaaaat gatagctttg ttcattccct
    29941 actacatata gagattgttt gaagtcgtaa gtacgtttac ggtacaatgc agcagctaag
    30001 tcacgtgtaa tgtatagtgt cgcaccgtct gacttcttaa tcaacgctgg attcaggtca
    30061 taagcagata ggtcaacgat ctctgcacct ttatcctcgt tcagcaaatg tttttcttct
    30121 aaaagtttga cgatttcatc catcttgtca ttgtagaaag cttcaccgtt caacgaatcg
    30181 aaacgtactt ccagcagatc atagatttta ttgaattctt tcatcgattc atcacggaac
    30241 cattgccata attgaatagc ctctttatcg ccttcttcca gacgtttgaa ccaagcacga
    30301 gcttcttcat ttaattctgg ttctgtttca gctacttcat ggaattggac gtataaacga
    30361 agcaattcat tgattggttc tgctttaaca gcttcttctg taccccattt tttgtaagcg
    30421 acgatcagtt ttccaaactg tgttccccag tcacctaaat ggttgatgcg gattggctga
    30481 taaccgattt tttccataat gaaaccgatc gaattaccga taactgtcga acgcaaatgt
    30541 cccattgaga ttggtttggc aatattagga gaagacatgt cgattggtac tgttccttga
    30601 ttcccgatgt tgctatcccc ataatgttct ttttctttca ctactgtttg caaaactttt
    30661 ttgctgataa gttctttatt catgaaaaag ttcaagtaag gtccgacaac ttcgattttt
    30721 tcaaagttgg cagaatcgat tttttctgct aaatctgcag cgatttgctg tggcgctttt
    30781 cggtaaactt ttgctaatga aaaggctgga aatgcaacat ctccgtgttc agctgatttt
    30841 ggattttcta gtaattgctc gacttgttcc agtgttaaat catctttgac agcgtcatag
    30901 acagcttttg cgacaagatc tttattattc atctttattc ctcctaaagt gttcccttga
    30961 ttgttaaaca aaaaaaatct ctagcgacgg catacttagt atgccatcac tagagacgag
    31021 atttcccgcg gtaccactcc aattgtccta aaaaaggacc agctccatct gatatcggta
    31081 gacgaccgtc tgtttaaaac agaattctcc aagtgcgctt cgttgtatct tcttcattcg
    31141 gcttccacca cttccgaatt cgctagccaa gaatgataca agtactctct tgttcaacaa
    31201 atcttagtct tattaaactt tcttgaatta tatcagaaaa aaaaacgaaa ggctagtcgt
    31261 ttcataaaaa aacaatccag atggcctaaa ggagaagaaa taccacctgg atagctataa
    31321 atcatttcgt aaatacgaaa gacttaacaa attaatcagc tacgataatc gttccttctt
    31381 cagaagctaa aagattctcg atattttcta aagaagtgat gattgcctta gcgtttggtt
    31441 ttgcttcaac aaatgcaata gctgcttcta ctttcggaag catgcttcct ggagcgaatt
    31501 gcttttcatc aatgtattgt ttcatttctg aaacagtaac agtttctaat ttcttttgat
    31561 ctggtttttg gtaattgaca tacacattgt ctactcctgt caatacgatc agcaaatccg
    31621 catcgatgat ttcggctaat ttttctgaag caaaatcttt atcgattacg gcttctcgtc
    31681 cttctaatcc agtagctgtt tctacaacag gaatcccacc gccaccaaca gagactgtga
    31741 taactccttg atcgaccagc gtttcgatga cacgtgcttc tttaatcgag atgggtttag
    31801 gggaagcgac tacttttctc catccacgtc cagcatcttc tttgaatgtc acagtggagt
    31861 cagcattcat ttgttctttg gcttcttctt ccgtgtagaa tggaccgact ggtttacttg
    31921 gatttttgaa tgaaggatca ttttcatcca caataacttg agtgactaaa gaaaccacat
    31981 ctttatcgat ccctttttcc ttcagtactt cgcccatggc gttttgtaac cagtaaccga
    32041 tagatccttc tgtcattgca acacatgtat ctaaaggcat tgccggtgtt ttctcagaat
    32101 cagcagcttg ctgctgaatc aataggttac cgacttgcgg accatttccg tgggagatga
    32161 tcaattcatc tccttgttcg ataaatttca ctaaatactt tgctgtttcc attaacgctt
    32221 cttgttgggc ttttgcactt gcatccgtag acaagatggc attgccccct aaagcgacaa
    32281 ctacttttcg tttggccatt tcattcgtcc tttcttcagt tctaccgaat tttctagata
    32341 acaaaacgag ccagcgtttt gtgattttcc ctcaatgctg actcgttttt tcgtgagatg
    32401 aatgtggtta tgcttaaacg cgagggataa acaagttacc taatgttgct gccatgatcg
    32461 ctttgattga gtgcatacga ttttctgctt ggtcaaattg acgagcatat ttgctgcgga
    32521 agacttcatc tgttacttcc atttcagtga tgccaaagcg ttctttcatt tgttcaccgt
    32581 aaacagtgtt tgtatcatgg aaagctggca agcagtgcaa gaagatcaaa cgatctgtgt
    32641 tgtgtgtttt ttctaccatt tccatattga tttggtaagg tttcaacaat ttgatacgtt
    32701 cttcgaattt atcttcttca cccattgata cccatacatc agaataaagg acgtctgctc
    32761 cgtctacacc tttatcaaca tcgtcggtaa tcatcaattg tgcaccggat ttttcagcaa
    32821 agccttctgc cattttgacg atttcttctt caggttgtaa ttcttttggt gcaacgatac
    32881 gcatattcgc tcctaaaatt gcacctgtga ctaataaaga gttagccata ttgtttcgac
    32941 catctccgca gtaagcaaca gtgatacctt ctactgttcc aaagttttct tggatagtta
    33001 aaaagtcagc gatcatttgt gttggatgcc attcatcagt caagccattc caaactggaa
    33061 caccagagaa ttctgctagt tcctctacca ttttttgact gaatccacgg aattcgattc
    33121 cgtcaaacat acgtcctagt actttagctg tatcttctgt ggattctttt ttacctaatt
    33181 ggatatcatt tgcacctaga tattcaggat gtgcacctag atcgatcgct gcagtagtaa
    33241 atgcagaacg agtacgtgta gatgtttttt caaataaaag cgcaatattt tttccttcta
    33301 gatagtgatg tgggatacca cgttttttca agtctttcaa gtgttctgaa aaatcgatca
    33361 aatattgtaa ttcttctttt gtaaagtctt tttctgctaa taagcttctt ccttggaata
    33421 ctgattcttt catattaaac attctccttt agattgtttt tatttaagat taaagaggaa
    33481 atcccgacgg cagctgacag taagactgcc gtcaggaaac aaattacttc aagtcttcac
    33541 ggacaagtgg ttggctcatg cagcgtggac caccacgacc tcgtgacaat tcacttgaat
    33601 tgatttctaa cactttgatg ccatagctac gaagcaattc gttcgataca taatttcggt
    33661 tataagtgac tacaacacct ggagcgattg ccaatgtgtt tgatccatca ttccattgtt
    33721 ctcgaggtgc aacgatctca tctccattac cagttgggat cagtacaaga tcatctaatc
    33781 caagagcagc tttcatcgtt tcgtgtaagt tatctgaatg agtaatcttg atatcatcac
    33841 catctggttc gattgtaaag acatccatct taccgtcttt actttgaatt gctggatgga
    33901 tagtgaattt atcgtgatct accattgtaa atacggtatc caaatgcatc attgcacggt
    33961 tgttcggaat tttgattgcc aacacttttt caaatcctga attttttgcg aataggtggc
    34021 gtgctaattt ttctaaagct ttcgcattag ttcgttgaga aatcccaaca gctactactt
    34081 tatcgcttaa gatcaattcg tctccgcctt cgatatgatc tggattttca cgatccaacc
    34141 atacttcgat tcctttattt gcaaatcttg gatgatgttt caaaatatat tctgtaaaca
    34201 tcgattcacg acgacgtgct tcaaaagtca tcttattgac tgtcatccca tttcccattg
    34261 atgcggaagg gtcgcgtgta aagtacaagt ttggcattgg gtccatgtaa aatggatagt
    34321 catcgtctgc tgacagatca tatagactgc tagaacgtac ttcgatatct tttgtacgaa
    34381 ccccagccat gattttatct accatttctt gcgtttccat tgatagaagg aactcatgaa
    34441 gaccctctct tacagcattc gatgcaatat gagattcatc caacatttta ttcaagaatt
    34501 gttctttgac attgcctgcg tcaatcgctt ctgccgctaa tttttccaag taaagagtct
    34561 cgacgccttc attttgcaat gtcttcgcaa agttgtcatg ttcttcttga gcaatcggaa
    34621 gatatggaat atcatcgaac aacagacgat ccataatatc tggtgtcaaa ttttccactt
    34681 cttgtccagg ccgtttcagt aaaactgtct ttaatttccc tatttcagag aaaacgtgaa
    34741 taggtttatc catgttgtgt tgcctccttc tttttgatta catgtatagg atatcctcct
    34801 tttgtaatca ctttcataat tttgtctcat tcaaaatgag acattcctca cattaatatt
    34861 cgataattaa ataattaaac gtttttctat ttttaagcgt aatttattca ttttttattc
    34921 tgtatacact ttcaaaaaaa tatttattca ttgaaaaaat ttttttgaat aaattaaaaa
    34981 aataaattat attatcgttg ttagcgttta ctatatgtat tattgttgtt ttttatacac
    35041 aaatcattcg cttcaaaaca aattattcaa taccattgta tattttttac ataataacaa
    35101 attatatttg taaagtatta aaacttgttt ctaatgttgg tatgttaaaa ctttacaaat
    35161 tctttaaaaa aagtgacaat aaatatagtg aacaataagg agaggacttt ttaatgcgtg
    35221 ttaataaaat cgattttcac ttttcaaaac tcagatatca acctgatttt gaacatttct
    35281 cggatgaaga gttccaacag atcaaggaac atactgttct tcgttcttat aaaaaaggac
    35341 agatcctttt cgacgatggt gatcccagac aacggttgta tatcttgacc aaaggactcg
    35401 ttcgtttaga acgttatgat gaaagtgcaa cttattacta ctatgactat gtgaatacaa
    35461 ataacctttt tcctatgtca ggaatgtttg gagaagagac ctacacttat acagctcaag
    35521 cgatgactga tattgaaaca ttctaccttc ctgtttccta ctatgaaaaa ttagcacgaa
    35581 aaaatcccga acagttgatc tatatgatac aaaaaatttc taaagtagtt gaaatacatg
    35641 aattgcgtat ccaaatcgga ttgacctcta gcgcctttga ccgggtaaaa caaaatttat
    35701 tcattttgaa agaagaattg ggcgtaccaa caaaagatgg tcatatcacc attccttatc
    35761 cgatcacctt aaaagaatta gcagtgaata gcggcacgac acgtgaaact gctggacaag
    35821 taatcaaaca actacgtgat tccgggctgt tagattatga caaaaagcgt tttactttct
    35881 gtaagacagc tactactcca gctatgagag aataacgcga acaaaaataa gcagtgaccc
    35941 tgataaa
//
LOCUS       DANKKG010000015        35059 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000015 DANKKG010000000
VERSION     DANKKG010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.015"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            21..1682
                     /locus_tag="PW004_000619"
     CDS             21..1682
                     /locus_tag="PW004_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2725613.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(1752..2099)
                     /locus_tag="PW004_000620"
     CDS             complement(1752..2099)
                     /locus_tag="PW004_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HDL2725614.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSTLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2183..3124
                     /locus_tag="PW004_000621"
     CDS             2183..3124
                     /locus_tag="PW004_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2725615.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3125..4168
                     /locus_tag="PW004_000622"
     CDS             3125..4168
                     /locus_tag="PW004_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2725616.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4174..5244
                     /locus_tag="PW004_000623"
     CDS             4174..5244
                     /locus_tag="PW004_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2725617.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5234..6187
                     /locus_tag="PW004_000624"
     CDS             5234..6187
                     /locus_tag="PW004_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2725618.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6199..7209
                     /locus_tag="PW004_000625"
     CDS             6199..7209
                     /locus_tag="PW004_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2725619.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7494..8315)
                     /locus_tag="PW004_000626"
     CDS             complement(7494..8315)
                     /locus_tag="PW004_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDL2725620.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8412..8690)
                     /locus_tag="PW004_000627"
     CDS             complement(8412..8690)
                     /locus_tag="PW004_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725621.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            8958..10967
                     /gene="metG"
                     /locus_tag="PW004_000628"
     CDS             8958..10967
                     /gene="metG"
                     /locus_tag="PW004_000628"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HDL2725622.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11446..12033)
                     /locus_tag="PW004_000629"
     CDS             complement(11446..12033)
                     /locus_tag="PW004_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725623.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12208..13530
                     /locus_tag="PW004_000630"
     CDS             12208..13530
                     /locus_tag="PW004_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2725624.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13685..14461
                     /locus_tag="PW004_000631"
     CDS             13685..14461
                     /locus_tag="PW004_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HDL2725625.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14454..15023
                     /gene="rnmV"
                     /locus_tag="PW004_000632"
     CDS             14454..15023
                     /gene="rnmV"
                     /locus_tag="PW004_000632"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HDL2725626.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15016..15900
                     /gene="rsmA"
                     /locus_tag="PW004_000633"
     CDS             15016..15900
                     /gene="rsmA"
                     /locus_tag="PW004_000633"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HDL2725627.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16351..16980
                     /locus_tag="PW004_000634"
     CDS             16351..16980
                     /locus_tag="PW004_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725628.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17104..18213)
                     /gene="ugpC"
                     /locus_tag="PW004_000635"
     CDS             complement(17104..18213)
                     /gene="ugpC"
                     /locus_tag="PW004_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HDL2725629.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18422..18844
                     /gene="mgsA"
                     /locus_tag="PW004_000636"
     CDS             18422..18844
                     /gene="mgsA"
                     /locus_tag="PW004_000636"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HDL2725630.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            19167..19703
                     /locus_tag="PW004_000637"
     CDS             19167..19703
                     /locus_tag="PW004_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HDL2725631.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(19743..21506)
                     /locus_tag="PW004_000638"
     CDS             complement(19743..21506)
                     /locus_tag="PW004_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2725632.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21509..23224)
                     /locus_tag="PW004_000639"
     CDS             complement(21509..23224)
                     /locus_tag="PW004_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2725633.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(24003..24227)
                     /locus_tag="PW004_000640"
     CDS             complement(24003..24227)
                     /locus_tag="PW004_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725634.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            24418..27195
                     /locus_tag="PW004_000641"
     CDS             24418..27195
                     /locus_tag="PW004_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL2725635.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            27276..27695
                     /locus_tag="PW004_000642"
     CDS             27276..27695
                     /locus_tag="PW004_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HDL2725636.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            27707..28177
                     /locus_tag="PW004_000643"
     CDS             27707..28177
                     /locus_tag="PW004_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2725637.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            28194..28973
                     /locus_tag="PW004_000644"
     CDS             28194..28973
                     /locus_tag="PW004_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2725638.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            28960..29781
                     /locus_tag="PW004_000645"
     CDS             28960..29781
                     /locus_tag="PW004_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL2725639.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            29798..30847
                     /locus_tag="PW004_000646"
     CDS             29798..30847
                     /locus_tag="PW004_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL2725640.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            30860..31864
                     /locus_tag="PW004_000647"
     CDS             30860..31864
                     /locus_tag="PW004_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL2725641.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            32497..33516
                     /locus_tag="PW004_000648"
     CDS             32497..33516
                     /locus_tag="PW004_000648"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDL2725642.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            33559..34440
                     /gene="galU"
                     /locus_tag="PW004_000649"
     CDS             33559..34440
                     /gene="galU"
                     /locus_tag="PW004_000649"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDL2725643.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            34740..>35059
                     /locus_tag="PW004_000650"
     CDS             34740..>35059
                     /locus_tag="PW004_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725644.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTT"
BASE COUNT        11461 a         6189 c         7220 g        10189 t
ORIGIN      
        1 ccaataaagg ggtacgtaaa atgaaaaaga aatttacatt tggtttggtt gcggtatgcg
       61 gccttgtact tgctggatgt tatggcggca gttcagccac aagcagtagt tcagcaaaaa
      121 gcggttcagc agatggagga ggcgtattta acttagtcgt gcctcaagaa atgcctacag
      181 ctgacttatc agttgcaaca gatacaatca gttttacagc attgaataac gtttatgaag
      241 gaatttaccg tcttgatgaa gacagcaaac cacagccggc aggagctagc gagttggcag
      301 aagtcagtga agacggatta acttacaaaa tcaaattacg agaagacgca aaatggtcaa
      361 atggtgatcc tgttacagca gcggattacg tttatggctg gcaacgaaca gtagacccag
      421 ctacagcttc tgaatatgct tacctgtatg cgccagttga aaatgcagaa gacattacag
      481 ctggaaagaa agataaatca gaactaggga tcaaagcagt tggtgactat gaattagaaa
      541 tcaaattgac gaaacaaaca ccatatttcc aatatctgct tgctttccca tcatttttcc
      601 cacaaaaaca atccgtagtg gaagaaaatg gggatgccta tgcatctgca agtgataaag
      661 ctgtttataa tggaccattc acattagaag attttgatgg accaggaaca gatacagaat
      721 ggacttataa gaaaaatgat cattactggg acaaagatac agtaaaactt tctgaaatca
      781 aagtcagtgt agtaaaagaa tcttctactg cattgaactt attcaaagat ggccaagcag
      841 atgacgtgat cttgtctggt gagttagcac agcaaaacgc aaacgatcca gcttatactt
      901 ctgttaaaga agcgcggaca agctatatcg aattcaatca acgtgaaaag aattcaccat
      961 tcaacaatgt gaacctaaga aaagctattt cttattctat cgatcgtgaa gctttagtaa
     1021 aacaagtact aggtgacgga tcagttgtct ctacaggatt gatcccatca gatatggtaa
     1081 aaagtccaac aacgaacgaa gactttgcaa aagaagctgg aaaattagtt tcttatgaca
     1141 aagataaagc aaaagaatat tgggaaaaag cgaaaaaaga attgggcgtt gattctttag
     1201 aatttgattt gatggcttct gatgatgatt catcgaaaaa agtaatcgaa tatgttcaaa
     1261 actcaatcca agaaaacttg gatggcgtaa cagtcaaacc aacacctgtt ccattctctg
     1321 tacgtcttga ccgctcaact tcgggtgatt ttgacgcagt actaggcgga tgggctgctg
     1381 actatgcaga cccaagcagc tttactgatt tgtttgtcac aggtaattca tacaaccgtg
     1441 gtcgctggag caatgcagaa tatgacgaag cagttaaagc agcttcatca acagacgctg
     1501 gcgatgaaca agcccgttgg gatgatttcc aaaaagcaat caagatcatc tctgatgata
     1561 tgggtgtcgt tccggtgtac caaaaagcag aaggtcacct agtgaatgaa aaaatcaaag
     1621 gtatcgttca ccatgcggca ggcgcttcat gggattacaa atggacatac gttgaagaat
     1681 aatcgttaat accgtgagca tgagaaaaaa cgaggctgcg gcaaatgtcg caacctcgtt
     1741 ctcatattca cttatcctaa caaagaaaaa agcatgaaaa tagcagaaag agcaattagt
     1801 attccggcaa tgatcagcca aaagctgtac ataccgtagc tggcagacga gagcgtggaa
     1861 aattctggtt ttttctttcg gttccttgtt cgtgcaagat ggaccgatcg ctgaaaatga
     1921 tcgaagaaac ccgaagaaaa gacccataaa aaaccgccga taatcagaaa cggcaatgac
     1981 cataagaata gatcattaga taattgaatg agtgtaaagg aatggttgat gagatttttt
     2041 attaaaataa ccagaatcag tagaaaagct actgcgtagg ggatagattt ttttttcatt
     2101 tgattgctcc tttatttttg gttcattatc taataatgta gcgaaacatc tgtcagaagt
     2161 caaaagctag ggggaaacat tagtgaatag ttatatcaaa tatgtattaa aacgtgtctt
     2221 tttcatgatc atcacgcttt ggttaattgc aacaatcacc tttttcttga tgcaattgct
     2281 gccgggcacg ccatatacga atcaggaacg tttgagtcct gaaacaatcg agatgctaaa
     2341 taaacaagtc ggtttggata aaccagttat cgtacaatat gggatttatc tttctaattt
     2401 gcttcaagga gattttggaa tttccttcca atttaaaaac cagccagtcg cacatttgtt
     2461 agctggaaga atcggaccat ccttgcagtt agggttacag gcaattatct ttggaacagt
     2521 cctagggacg attttaggaa cgatctctgc catgaagcaa aatacatggg cagatacatc
     2581 ttctacactc gtagcgatat taggtcgttc cattccaaac tttgtatttg ctgttttatt
     2641 acaatatatc tttgcaatca aattacaagt tttgccaatc gcaaaatggg atggctttgt
     2701 gtacacaatc ttaccaacaa ttgcgttggc catgtcgccg cttgctgact ctgctcggtt
     2761 tatccgtacg gaaatggtgg aagtactgca tagtgattat gtggaattag caaaagcaaa
     2821 aggattgagc cgttgggaaa tcgcctttaa acatggtctt cgaaatagtt tgattccatt
     2881 gatgacttta ttaggaccgt tagctgtagc attgatgacc ggatcactgg tggtagaaaa
     2941 tatctttgct attcctggta tcggggaaca attcgttaag tcaatcacaa caaatgatta
     3001 tcctacgatc atggctgtaa cgatccttta ctcatttatg ctgatttttg ttattttgat
     3061 cgtggatcta ttgtacggtc tagttgatcc aagaattcgt gtttcagaag ggagccgagg
     3121 ataaatggaa atgataccaa agcgttatga aacagttgca gatatccctg cggatgaatt
     3181 tcttccatta caaaaaaata ccgaagaaga acgtgaacag attactgcac cttctttgaa
     3241 ttttatccaa gattcatggc gccgtttaaa gaaaaacaaa gctgccgtta tttcaatggt
     3301 tttactattg gtcatcattt tgatctctgt gatcacaatc tttgtttctc ctcatgatcc
     3361 aactgcccaa aacgtggctt atatcaattt gcctcctcgt attccgggaa tcaatatcga
     3421 tggattgaat ggaaaagcaa tggttggtgg tgaacttgtt gataaatatg ctcaagcaaa
     3481 cgtcccttca aacgtaaatt tctttttagg aacagacggt ttgggacgtg atgtattaag
     3541 ccgcttattt atgggaatga gaatctcgtt gctgatcgca tttattgctg cattactaga
     3601 tgtaacgatt ggtgtagctt acgggttggt ttctggtttg ttaggcggac gtgtcgataa
     3661 cgtgatgcaa cgttttttgg aaatcttatc aggaattcca aacctcgttg tcatgatttt
     3721 gatgttggtc gtttttgaac caggaatttt ttcaatcgtg gctgctatgg ccatcacaaa
     3781 ctggatacca atggcacgta tcgttcgtgc acaaacaatg aaattaaaag atcaagaata
     3841 tgttttagct ggattgacat taggtgaatc aagaatgaaa attgcattca aacatatttt
     3901 gccgaatatc tctagtgtca ttattattca aatgatgttt agtattccat ctgctatttt
     3961 cttcgaagcc tttttaagct ttatcggttt aggtcttacg cctccgtcag cttcattagg
     4021 aacgatgttg agcgaagggt acaagacatt cctgtatctg ccttatctat tatggattcc
     4081 ggcagtaact ttatcggtta tcatgattgg ctttaactta cttgctgatg gattgcgtga
     4141 tgcctttgat cctaaaatga aagagtgaag acgatgacga aaattttaga agtgaaagac
     4201 ttagaaatct cttttgatac gtatgctgga aaggtacgag cgattcgcgg tgtcaatttt
     4261 catttgaata aaggagaaac attggcgatc gttggggaat ctggaagtgg aaaatcagtg
     4321 acgaccagaa gcatcatgcg cttgctgtca agcaacgcca atatagatgc tggtcaaatc
     4381 ctctttaaag gtaacgatat cgttggaaaa tcagaaagag agatgcaaaa aatccgtggt
     4441 aaagaaatag cgatgatttt ccaagatcca atgacctctt tagatccgac gatgcccatt
     4501 gggaagcaag tcgctgagtc ccttagaaag cataataaag tttcaaaaaa agagggacaa
     4561 aaagctgcat tagaattgct taaattagtt ggaatacccg aagctgagaa acgtatcaac
     4621 agttatccgc atcagttctc aggaggtcag cgtcaacgga tcgtgatcgc gattgcactg
     4681 gtatgttatc cggaaatcct gattgctgat gaacctacaa cagcgcttga tgtgactatt
     4741 caagcacaga ttttggagct gctgaaagaa attcagacaa aaatcgatac atcaattatc
     4801 tttatcactc atgaccttgg tgtagtagcg aatgtcgctg atcgtgtcgc tgtcatgtat
     4861 ggaggaagaa tcgttgaagt tggaacatcg gaagaaattt tctacaaccc gcaacatccc
     4921 tatacttggg gactgcttgg ttcaatgccg acattagata gtgcgaacga tcgattgtat
     4981 gctattcctg gttctccacc ggatttattg aacccgccaa aaggagacgc attctatcca
     5041 agaaatgaat tcgcgatgaa aatcgatgca gaacaagaac caccattttt tgaactatca
     5101 aaaacacaca aggcagcaac ttggctactt gctccgcagg cacccaaagt gacaccgcca
     5161 gaagaaatcc aacgtcgctg ggctatcttt aaagagaaac aaaaacatac ttacggtcaa
     5221 ggaggaattg ctgatgccaa ataaactact tgaagttaaa ggattgaaac agtatttcaa
     5281 tgtaggaaaa aagaatgaag tacatgctgt aaatgacatc agctttcaca tttatgaagg
     5341 agaaacattc ggtttagttg gagaatctgg aagcggaaaa tccactactg gccgtacgat
     5401 cattcgcttg aatgaaccta ctgatggtga gatattattc gacggtcaag atataacgaa
     5461 gatcaaagat aaaaaaggtt tgacgaaatt ccggcatgat gttcagatga tctttcaaga
     5521 tccatacgct tcgttgaatc ctcgtatgaa agtcagagat atcattgccg aaggaattga
     5581 tgttaatgga ctagctaaat catctgaaga gcgtgcaaaa aaagtcgatg atctattgag
     5641 aactgttgga ttgaatccaa gccacggtac tcgttatcct catgaatttt caggtgggca
     5701 acgccagcgt attggtattg cacgagcatt ggcagtaaaa ccgcgtttta tcatttgtga
     5761 tgaaccaatt tctgcactag atgtgtcgat ccaagcccaa gtggtcaatt tgttgcaaga
     5821 tctccaaaaa gaacatcaat tgacctatct atttattgca catgatttgt cgatggttaa
     5881 acatattagt gaccgaatcg gtgtaatgca taacggcttg ttattagaga tgggaacaag
     5941 tgatgaaatc tataatcacg gtgttcatcc ttatacagaa agtttgttat ctgctattcc
     6001 tctaccagat ccagaccatg agagaaaacg cagaagaatc aaatatcaac ctgaaccaga
     6061 tgacggacaa gtacgtcaac ttcgtgaaat cgcaccagaa cattttgtct atgcaactga
     6121 gcaggaagtc ccatattacg caaaaaaact aaaacgacaa aaggaatctc ttctggttgc
     6181 taactaggag ggacatagat gctaaaagct tttaaattcc gtatgtatcc gacagaagaa
     6241 caaaaacagc aattgattcg tacttttgga tgtgctcgat ttacctataa ccatttgctg
     6301 aaaaagcggc agaaaagttg gcaacaaaca ggagtagcca atttttcgct cacacccgct
     6361 acattaaaaa aagaataccc attcttaaaa gaagtagaca gtcttgcttt agcaaatgca
     6421 cagcttaatt tggatcgggc ttttcgcaat tatttcaagg gacgtgccag cttcccaaag
     6481 cttaaaacga aaaaaagtat gtggcaatct tatacgacca acaaccaaac ccgtacgatc
     6541 tatctggaaa atggctactt aaagttacca aaacaaaaag aactaatcaa gataaatcag
     6601 catcggccag ttgaaggatc gattcgctcc gctacgattt ctgctcgata taatgaagaa
     6661 ttttatgtcg cactactttg tgatgtttcg ccagtaaaaa aagaatcttt agctaaatgg
     6721 atcgggatcg cctatcatcc gaagacattg atccaaacat ctcgacctct agaagtaact
     6781 ttaccaaaat ttcaccagac agaagaaaag ctgcaacatg cacagcggaa attgaatgtg
     6841 aaagtccgct ccgctcacca tagaaagatc aggttagatc aggcaagcaa ttatcagaaa
     6901 caaaagagaa aagtaatgga tctttatctg aaacaaaaga accagcgaga agattatctt
     6961 gaacaattat ctgggaaatt ggtcaaacaa tatgactact tatttgtaga atcttttcca
     7021 aaagaagagg cacatgctga tttttctatt cacgactggc ataaattaat aacaaaattg
     7081 cgttataagt cccaatggta taataaaaaa tttcttttga tcaataccga cggagcagaa
     7141 gaaagcaatt ccgtaagaaa gagccaagta gtagaaaaaa tgggcagaca ttcggttatc
     7201 aagggataaa aaatgaatga aactgatagg agcgttaggg ttagccttgt taataaaaag
     7261 tcttaatgac ttttgtttct aggaattttt cttttgaaga aaagctcact aattatgaca
     7321 aaccaataaa acaatagcag aaaaagttcg tacgagcttt ttctgctatt gttaattttt
     7381 tattcagaaa gtttacgaat acatcaaaat tctgaaacaa aaaacgtaac tcacaaagag
     7441 ttgtttttgc gttttttctt gaatgattag gtctgaatct tttgctcaca accttattca
     7501 tggtgccccg gcttgagctg atagattttt gaaggacggc cgattcctac aggacgttca
     7561 ccgatctcat cgaaatattg gagcatagct ttcttaaaat tggaatgatc gatcgtttta
     7621 taatcgatcc ctaagaattt agcaaatact tttcgagctt ccgtaatcgt gaaatcttta
     7681 cctaaaactt gtagaacttg cggctcgtgt tccattttat tgactacacg gttgaatgct
     7741 ttcaagatga tttgactatg gtcaaaagcc aaagtatctt tgccagaaga ctctccagtg
     7801 actaaatcaa gcgagatagc cacttctccg ctagataaat ggatcttgtt actatgacgt
     7861 tctagtgtga accagcgtac ttccttggca tcatctccgg cgattagcgg ttcttctcca
     7921 ataaaggcta agtagctgac tgtgacgacc cagcctcgcg gatctcgatt aggcgtactg
     7981 aatgtatgca gttgctcaat attttctttt gaaataacca caccagtttc ttcctttgtt
     8041 tctcttaaga cgctttctcc agttgactca tccttttgga caaagccacc aggcaatgcc
     8101 catgaatttc gataaggatg cccttttctt tggatcaaca aaactttcag ctgatcttct
     8161 tctttgttat agcacatcag tacgatatcg acagtcaaag agggcttgtc atattctgga
     8221 cgttcctgtt tttgatacca gttaagaaat tcttcttcac ttgctttatg ttcataatat
     8281 aatttttctt cttctttaga agcaaaacgg ttcatctgac cacctccatt gacagtaaaa
     8341 gtacttttta ccttattgta gcagactctg aaaaaaaacc aaggatttac ttgaaaaatc
     8401 taggttatca tttaaatatg aaaaataaat ggaagtatga gattgaataa aaagaaaagc
     8461 agataaaacg gcaatacttt ataccgctct tcacgaatgg caaagaagcc acaaataacg
     8521 atatatacgt caatgataaa acgcaaaatc caagctgctg tcataaaact gttcaaatgc
     8581 ccatggaggt taaaataacc atacatacga atataaaaat agctgaatat taaatgtgct
     8641 aatgaaagaa aagcaatact aagtgtaaaa aaaagatgtt tcttctgcat gattgttcca
     8701 gtcccttact aagatagata gtctttatta taccacgtgt gaggggagaa ataaaaagcc
     8761 gttttttagt agatatgaaa caaaacaaaa acccgcattt attcaaccta ttcatccttt
     8821 taaaggatag attgaacgta gatagaagtc gtttatccag tctttttcat tttgaaaaaa
     8881 agctaacagc atgctttttt tatggtatac tagcaaggaa acgaattcgc atcatgcgat
     8941 aaaggaggaa gagccccgtg gcagaaaagg aaacttttta tatcactacg ccaatctatt
     9001 atccaagcgg caaacttcat atcggcaact cgtatactac gattgcatgt gacgcaattg
     9061 ctcgttataa acggttgatg ggctttgatg tgttttatct cactggagtg gatgaacatg
     9121 gtcaaaaaat cgaaacaaaa gcagaagaac taggtgtaaa accgcaagaa tatgtagata
     9181 aaatggcggc tgatgtcaag aaattatgga aaacgcttga tatcagttat gataaattca
     9241 ttcgcacgac agatgattac cataaagcag ctgttcaaaa aatatttgat cgtttattag
     9301 aacagggaga tatttactta ggtgaatatg aaggctggta ctctgtttct gacgaagaat
     9361 tctttacaga aacacaactt gcagaagtgt acaaagatga cgaaggtaac gtgatcggtg
     9421 gtaaagcgcc aagtggacac gaagtagaat tggtaaaaga agaatcatac ttcttccgca
     9481 tgagcaaata cgcagaccgt ctattggaat actatgaaga acatcccgaa ttcatccagc
     9541 cagaatctcg taaaaacgaa atgatcaaca actttatcaa accaggatta gaggatcttg
     9601 cagtttcacg aacaactttt acttggggag taccagtcaa gagtgatccg aaacatgtgg
     9661 tgtatgtatg gatcgatgca ttatccaatt atattactgc actaggatat ggctccgaag
     9721 atgaaagtct attcgaaaaa tactggcctg cagatgttca gatggtcggt aaagaaatcg
     9781 ttcgctttca tacgatctat tggccaatca tgctgatggc ccttgatttg cctctaccta
     9841 aaaaagtatt tgggcatggc tggctattga tgaaagacgg aaaaatgtct aaatcaaaag
     9901 gaaatgtcgt ttatcctgaa atgcttgtgg aacgttatgg tttagatgca ctccgttatt
     9961 atttgcttcg tgctgttccg tttgggtcag atggtgtctt tacgccagaa gattttgttt
    10021 cccgtgtcaa ttatgatctg gcaaatgatc tagggaactt attgaatcga acgattgcta
    10081 tgatcaacaa atattgtgat ggactcgttc ctgactatgc ctcacttgtc actccatttg
    10141 atagtgaact gtctacgacg gctgctaacg ttgtcggacg ttaccatgat gcaatggaaa
    10201 aaatggaatt caatacagca ttggctgaaa tctgggtgct gatttctcgt gcaaacaaat
    10261 acattgatga aacagaacca tgggtattag caaaagacga agaaaagaaa gctgaattga
    10321 atagtgtcat gatccattta gctgaatctt tgcgtatcgc agcgatatta cttcagccaa
    10381 tcatgacaga aacgccagag aagatcttca atcaactagg attggactct gaaacaatga
    10441 atcttgaagg attacacttt ggcgaatttc cttcaggaac aaaagtagtt gcaaaaggta
    10501 caccaatctt cccacgttta gatatggaag aagaagttgc ttttattcaa gaaaaaatgt
    10561 ctgaaggtac tcaaacaaac gaagatacag tcaaatggga tccagaagaa acagaacttg
    10621 tttcaacgaa agaaaaacaa atcaaatttg atgtatttga aaaagtagaa ctaaaagtag
    10681 cagaagtcat caactgtcaa aaagtagaag gggcagataa gttgctgcaa ttccgactag
    10741 atgctggaga cagccaagac cgtcaaatct tgtcaggaat cgctgaattt tatcctgatc
    10801 caagcgaatt gatcgggaaa aaagtcgtca ttgtagcaaa tctgaaacca cgaaagatgc
    10861 gtggacaaat cagtcaagga atgatccttt ctgcagaagc accagatggc tctttacaag
    10921 taatcgaagc accaaaatcc atgccaaatg gctcagaaat cgcatagaaa ttcaaagggg
    10981 gtatgacact agttgtgtca tacccccttt ttccgaaatt tgagaagcaa ttttcgtaaa
    11041 ttccttctta ttacttgaag ccaatcatat aatacagaaa cagagaacta tccaaaataa
    11101 atcctagaaa aagaagtacg agcttcgctg atttttgtta atttagaata atactaatag
    11161 gaatcgtaaa aacagaaaaa aagcataatg ataaagggtc gggcagattt taattgattc
    11221 atctggtact cttttttatt gtccttacat tcataaagtt tgaatgcaat gactttatta
    11281 catctatatt tatatctaac agtagcgttg atgtgttaat tataagttct atcactttat
    11341 gttcaattag tttgtgctct tatatgtgtg attgaaaata tagataaaaa aacaatcatc
    11401 ggctttgact tcgatgattg ttttctttat tttgtaaaaa tcctatcact tgattttatg
    11461 gtaacctttt atttttgtct tcaaacggct tggtaagaga gcagtaagca gtgcaaatgg
    11521 gatcgaaaag acataccaga aaatcgtacg tccgatacca taatgttgat agagctttgt
    11581 caacatcacg tgtaacaaga tcaaccctaa aagcaaggag ccagctataa accaatgatt
    11641 atcccaatgc atgacttcac taagtgccat cactccaaaa ggatagaaat aaaaatagat
    11701 cccgactaag gtcatccgcc agttctttgt aaatgttccg atattcaata aaaagaaaat
    11761 cgctgaaagc aaaataccga taggcggaaa aagcccaacc aaaaaggaat acaacatccc
    11821 ccacaagatc atcacaggac ctttgatcaa tgcaacgatt gcgatgaaca aagtcagcaa
    11881 tattagtttt tgtgataacg ttgcaacagt cattaatcca aataaaatta atgtgaacca
    11941 atgccaatgg gactgctgtt gttctttacg ggaaaaatcg acccggttgg ataaataatc
    12001 tgctaatttc ggataagaac gcttaaaatc cataaactgt tcctccttcg tggaaaaggt
    12061 aggtttagta tacgaaaaat aaaaaagaaa tacatccgtc ttaagaggga gtttttcact
    12121 gaaagtggag aaaaaagaat ggtaaattaa aatattctca gttatactga aaattatacc
    12181 aaatttcaaa gaagaaagag gatgggaatg aaaggcaaac taaaacgaga aatcaatctg
    12241 tttggcgcat tagctaccgt tatgggaacc gtgatcggtg caggcgtctt cttcaaaact
    12301 gctgctgtta ctgctagtac tcagtctaca agtttgacat tgctagcttg gcttttgggt
    12361 ggtttcttaa cgatctgtgc tggattgaca gtggctgaat tagctacagc aatcccggaa
    12421 actggtggag ctgtaaaata tattgaagct gcgtatggaa aattgcccag ctttttatta
    12481 ggatgggcgc aaagcttgat ttactttcct gctaatattg ctgcattatc gattatcttt
    12541 gcgacacaat tgacgaactt attacaatta tctaccgatt atcttttgtt gattgcagtg
    12601 atcaccgctg tttctgtgac agggttgaat ttgttaggga caaaagtcgg tgcttctgta
    12661 caatcagtga cgttgattgt taaattaata ccaattgctg tgattgtcat atggggatta
    12721 ttgaccccag gacaaggaac tgtccagtta tttccaatcg aagctggaaa agatgttact
    12781 tttgtcgaag gattgagcag tgcgttgtta gcaacgttgt ttgcttatga cggctggtta
    12841 ggtgtcggtg caatggcagg ggaaatgaaa cggccggaaa aagatttgcc taaagcaatt
    12901 attcttggtt tgagttttgt aacagtcgtt tatctattga tcaattttgt ttttttgaaa
    12961 actttgccga ttgatcatct tgccggtaat ttgaatgcag cttctgaagc atcagacgtc
    13021 atttttggag gaatcggtgg aaagctggtc actattggga tcttgatctc ggtatacggt
    13081 gcattgaacg gttatacact aactgggatc cgcgtgcctt atgcgatggc tttagaagac
    13141 gagctacctt tcagtaaaca gctgacaaag ctttcaaaga agtttacggt tccttatgtt
    13201 cctgcggttt tccagttagc agtagcttgt attatgatga gccttggttc tttcgacttt
    13261 ttaacagaca tgttgatttt tgtgatgtgg ctattcagtt tactgatttt tatcggtgtc
    13321 tttattttaa gaaaaaaagc gccggaattg ccacgtccat ataaagtacc actttatcca
    13381 atcgttccga ttattgcgat tctcggtgct attttcattt taggtatgac gatgatgacg
    13441 caaacgaaat tagcattgat cggtataggt gttaccttga taggtatccc agtctattat
    13501 caaaaaaaga aaaaacgctc agaagagtaa aatatttcaa caacagacag aaaaagtacg
    13561 aactaggtcc tctagatctt cgtgcttttt ctatttcaat aaaacaagtt cttctatttt
    13621 gatttttgct acttctgcaa gtagtgctta tgtgctaaag taagaatgaa aaggagagat
    13681 aaacatgata tttgattctc atacccattt aaatgctgaa cagttcaatg aagatattcc
    13741 agaaacgatt cagcgagcac aagaaatggg ggtaacgaaa atggcggtcg tgggcttcga
    13801 tacgccaacg attgaaaagt ctttgcaatt aagccatgac tatccaaata tatacagtat
    13861 tatcggctgg catccgacag aggctggcag ttatacgaaa gaaatcgaaa ataaattaca
    13921 agaacaattg actttaccaa aggttgtagc attaggggaa attggcttgg attatcattg
    13981 gatggaagac ccgaaagaag tccaagataa agtatttcgc agacagattg ctatcgcaaa
    14041 agaaatgaac ctaccaatca gtattcatac tagagaagca cttgaagaca cgtatcaaat
    14101 cctaaaagaa gaagatgttc gagatatcgg tggaatcatg catagtttca gtggtgatta
    14161 tgaatgggca aaacgatttc ttgacttagg gatgcatatt tcatttagcg gtgtcgtcac
    14221 tttcaaaaaa gcattagacg tccaagaggc tgcagcaaat gtaccaatcg aacgattact
    14281 tgtggaaaca gatgcccctt atctggcacc agttcctttt agaggaaagc gaaatgaacc
    14341 aggctatact cgttatacag tagaaaagat tgctgaactt cggaatcttc cgacagaaga
    14401 agtggctgcc caaacatgga caaatgcgca ccgattattt ggtctaagcg aacatggata
    14461 agatgaagat ccaagagatt atagtcgttg aaggaaaaga cgatacaaga aggctgcggg
    14521 aagtagttga tgcagatacg atagaaacaa tagggtctgc aatcaatgaa gagatcctga
    14581 tgcaaattga acatgcccaa gagactcgtg gagtgatcgt ttttaccgat cctgattttt
    14641 caggagaaaa aataagaaaa atcattatgg aagctgttcc tcaggcaaaa catgcttttt
    14701 tgccgcgcac acaagctaaa ccaaagaaaa agggaagcct tggtgtcgag catgcaagtg
    14761 atgctgctat tttagaagca ttgaaaaagg tcgtgacgcc agacatttcc caaacggctg
    14821 tttctgaaat cactagacaa gaccttttag actatggttt aatagctggg gcacaagcaa
    14881 aagaaaaacg agaaaaacta ggtgacgagt tgcgcatcgg ttacacgaac ggcaaacaat
    14941 tggaaaaacg tttgaagatg tttcgtatta cgccgaaaga actcgcccaa gcaatgaaaa
    15001 tagtggagga acaacatgag tgaatatcgt gaaattgcga caccctctag aacaaaagag
    15061 attttattga aacatggttt ttcatttaaa aaaagtttag gacaaaattt tctgactgaa
    15121 ccaaatattt taaggaaaat cgtagaaact gcgcaaatag atgaacatac gaacgtgatc
    15181 gaagttggac caggaatcgg tgcattgacg gaacaattag ccaagcatgc aaaacaagtc
    15241 gtagcatttg agatcgatga tcgtttgatc cctgtattgg ctgatacgat gcaaccctat
    15301 gacaatgtga ctattgtaca tcaagatatt ttaaagaccg atttgtcaac agctgttaga
    15361 gaaacatttc atgaggagct tccgttaaaa gtcgtagcga atttacctta ctatattacg
    15421 acaccgatca tgatgcattt tcttgaatca gatttggttg tggatgaact agtcgtcatg
    15481 atgcaaaaag aagtagctga tcgtatctct gctgaaccag gaacgaaagc ttatggttca
    15541 ttatcgattg ctgttcaata ttatatggaa gccagtttag cattcatcgt accaaaaaca
    15601 gtatttgtcc ctcagccaaa tgtagattca gcgattttga aactgacaag aagagatata
    15661 ccggcagtcg aagtgacaga cgaaaaagca tttttccgat tgacaaaagc ggcatttcag
    15721 caaagaagaa aaacgctttg gaataacttg cagcattcgt atggcaaaga tgaccagaca
    15781 aaagcatggt tagcaaaaag cttggaaaca gctggaatcg atccaaaacg tcgtggggag
    15841 actttgtcat tacaagaatt cgccgcatta agcaatgcga tgtcagaaaa tatgcagtaa
    15901 aaaaagaggt tgtgacagaa gttgtgaaaa agctgtcctg catcaagtaa taagaacagc
    15961 caaacaaaaa tagttcctca tgtttttcgt ttgtttgggc ttattaccgc agatgcaagc
    16021 ttttgaacac cgtttaagaa agaggttgtg ggcctgccgt ttaactctaa gtcacaagga
    16081 acaatttcaa aaaacagctt ctcatgtttt tgagttagtt ggtcttatta cttgatgcaa
    16141 gcttttgacc actattatgc cgaacaagga gatgtgacac aaattttgtc acatctccta
    16201 tttttgtttc ttgatcaaaa aattcttata cttgatactc acactcattt tgtatggatt
    16261 tagaaaagaa tatgtgtctt gacagttatt agtaagtgag ttatactaag tcgacattta
    16321 tgtgtcaaga catattagga gagagaaaaa atgagaaaaa attcagttcg aaaactaacg
    16381 atttctgctt tattgattgg gatgggggtc attatcccaa tggtcatgcc taaaatcatg
    16441 attccaccag cttcctttac tttggcgtct catgtgccat tatttattgc gatgtttttt
    16501 acaccaggag ttgcagtggc tgttgcgcta ggtacgacat ttggtttctt tttaacaacg
    16561 ccggttatca tcgcattgcg agcattgtca catcttgtgt ttgcgttgat tggggcttgg
    16621 tacttgcaaa agcatccgag tatcgtttta aaaaatggac agtttacttt tgctaattgg
    16681 cgtttccaag ggttcaattt tgtgatcggt gtgattcatt ctttagcaga aatgctagta
    16741 gtcagcatct tttactttat tggtaatatg cctgaaacat attattctca aggatatttt
    16801 tatacagtat tcatcttgat ggggattggt gggcttatcc acagtttagt tgattataat
    16861 attgcttatt ttatagcagg aacactcagc aaatattttg atatccccgt atttacggca
    16921 gcaaaaaaag aaaatcggaa agagtcgatt caagggtacg cgaagaaaac gacatattag
    16981 acatgatctc tcaaaaaaaa aactctcatc tagtaatgga taataaatcc tctaagatga
    17041 gagttttttg tcctaaaaag agctgcacca acatcttctg gcacagctct aacagggggc
    17101 aatttatgga agtttgatga cttgttctgt ttctttatca aagaagtggc ctttgctgat
    17161 gttgaaggca aggtcgacta tttcgcctgg tttatggaaa tctcttgcat ctacttttga
    17221 gatgaactca gtagaaccag tttttgtgta aagcatcgtt tcagctccaa gtaattcgga
    17281 aacgacaact tctgatttca ctacggcttc aggtgaagct tcaagggcta cttgttcact
    17341 gtggatgtct tctggacgaa taccgaagat aacttctttt ccttcataac ctttttcaac
    17401 taatactttg ttttttcctt caggaatacg aagcttaagc ccatgtccat ctgtgataac
    17461 accatttttc aatgtcacat taaagaagtt catggcagga gatccaataa agccggcaac
    17521 gaacatattg acgggtgtat cataaacttc tttcggtgta ccgatttgtt ggataaatcc
    17581 gtctttcatg ataacgattc ggtcagccat agtcattgct tctgtctgat cgtgagtaac
    17641 atagatggtc gttgtttcaa gacgctggtg aagtttggca atctcagcac gcatggctac
    17701 acgtaattta gcatctaaat ttgataatgg ctcatccatt aagaagactt ttgcatcccg
    17761 aacgattgcc cgtcccaatg ccacacgttg gcgttgtcca ccagataaag cagctggttt
    17821 tcgtttcaag tattctgtca gacctaagat ctctgctgca ttgtccacac gttttttgat
    17881 gtctgcttta tcatatttac gtaatttcaa gccaaaagcc atattatcaa aaacagtcat
    17941 atgaggatac aatgcgtagt tttggaaaac catggcaata tcacggtctt taggtgccac
    18001 gtcattcatg acttttccgc cgatatttag ttctccttca gagatatctt ccagtcccgc
    18061 gatcattcgc aaagtagttg actttccaca accggaagga ccaacaaaaa cgataaattc
    18121 acggtctttg attgatagat taaaatcagt tacagaataa ttttctgcat tgtcatattt
    18181 tttatatacg tttttcagag ccatttctac cataatacgt tccacacttt cttttttagt
    18241 tttattaact atttaaagta taaatgaaag cgttaactaa cttcaatgtc atgttgaaca
    18301 agaatttttt cttttttttg tcaatgtgct agttttaagc tttaataaat atccataaga
    18361 aataaaaact gatacactgg agtaaataag tatgttaaca atatcaaaag gagtgtctct
    18421 tatgaaaatc gctttgatcg ctcatgaccg aaaaaaacct atgatggtga aattagcaac
    18481 agcgtataaa acaatattgg aaaaacatga actttttgct accgggacga ctgggcagag
    18541 gatcacggaa gcaaccggct tgccgatcca tcggtttaaa tccggaccgt taggcgggga
    18601 tcagcaaatt ggagccatga tttcggagga tcagttagat ttggtgattt ttcttcgaga
    18661 cccgctggct gcacagcccc atgaaccaga tgtaacagca cttatccgtt tatctgatgt
    18721 ctatgagatt ccgttagcta cgaatatcgg aacagctgag gtgctactaa gaggactgga
    18781 agccggcttc ctagattttc gaagaatcgt ccatgaaata gacaagcatc cactttcatt
    18841 ttgatggata agcgtatgga aaaataacgt ttaatgttcg cctatatgta gctataaaag
    18901 ctaaacaaac tgataatatg cgatataata cgaacgtatt ggataatata aacaataata
    18961 ttagtttttc gtatttgcag taaatgtgaa aatatgctat agtttctgtg ggaaaagatc
    19021 ccaaaaataa tacagagtag aaaataaagc gttaagtgct aagaggatgg gatgttgcct
    19081 cttggacgaa ggacgtaatg ttcgcggttt tattttcgca ttcgctgcat gatacgatgt
    19141 agcagctctt aaaggagatg ctaaaaatga agaaacaccg gttagacgca cgtatgattg
    19201 ctattatggg cttattgatt gcattgatgg tcactttgtc ccgactggta gcaatcgaga
    19261 ccccatttat taaaatcagt gtcacattta ttccacaagt gatcatgggc attctttttg
    19321 gtcctttttg gtcagggata ggcgcagttt tagctgattt agtaggaatg gccctcttct
    19381 cgaaatctgc attctttatt ggatttacat tgaatgcatt tattgaagga gcaatctacg
    19441 gattcttttt ctatcgaaaa gagatcactt ggaaaaatgc cattctggct acgcttagtg
    19501 tcacgctgat catcaactta tttttgacac cattatggct tgcgttgatg taccatgtgc
    19561 cattgttcag ctgggtcgtt tgggcgcccc gcctgttaaa aacagtaata tggttaccga
    19621 ttcaatcaat tgccatttat tacgtaggac gtagtattcc ttacaaaaaa attttaagaa
    19681 gtttagcgat ccacgctaaa taaaaaacaa gcagtcttcc catcccagaa gactgcttgt
    19741 ttttattgtg ctgtgaattg tgcttgatag agccgtcggt aataaccatc tttttgaagc
    19801 agctcatcat gggtgcccat ttcgacaata gctccctgat ccatcactaa tatctggtcc
    19861 gcatttttga ttgtggacag gcgatgggca atcacaaaac tggtttttcc ttccatcatt
    19921 gctaagaagg cttgttggat ttttttctct gtcaatgtat cgactgagct ggtagcttca
    19981 tctaagatca acataggggg attactgatc atcgttcgag caatcgtcaa cagttggcgt
    20041 tgtccatctg atatctttaa cccttgaccg ccaatcttag tatctaattt ttccggcaaa
    20101 cgttctacaa attcatacat ataagaggct tttagggcat cattgatttc tgcgtcactt
    20161 gcttccggat tcccaaaacg aagattttct cgcaatgtac tgtcaaatag ccaagtatct
    20221 tgtaacacca ttccaaaact tttacgtaat gtatcacgtt ggatagagcg gatgtcatgg
    20281 tcatcgactg taattgttcc ttttgttgtg tcataaaaac gcatcagcaa attaacaagt
    20341 gtggattttc cggcccctgt ttttcctact atagcaatgg tttgacctgg ataagcagta
    20401 aaattgaaat cttcgatcaa acgctgcttg gggtcataag aaaaagaaac atgatcaaaa
    20461 gagacttttc ctttgatatt tttgggttcc aaggcatagg catctggcaa atcaggcttt
    20521 tcttctggct gatcgatcaa atcaaatgct cgttctagac cagaaaaagc ggtttgcagt
    20581 tgtgtcgtga ttccagaaag ttcgataaat ggtttagaaa actgacttga gtagatcgta
    20641 aagctggaaa tcacaccaac agtgattgac gaaccactct tcaatgccaa taggccgcca
    20701 actaaaccaa tcgataaata agcaaggtga tctacaaaac gagaagacgg gtttgtcaag
    20761 gaagaagaaa attgtgcccg ttgtcctttt tcatatagat cagcatttag cagttcaaat
    20821 tgctgttgac tagcttcttc ttgctgaaaa gctttcacga ttttctggtt accgattcgt
    20881 tcggtcacaa agcccgagat atccccaata atcctttgct gtctatcgaa atttgtttgt
    20941 gaagcacgag caacgatcca actcacaagg aaaataattg gtgtagaaca aagaacgacc
    21001 aatgtcagaa gtggacttag acgcatcata aaaataaacg aaagaatgat gattgccact
    21061 ccagaaaata attgattgaa ggccgcagaa attgcaatgg aaatattgtc catatcattt
    21121 gtaaatcgac tgacgatatt tccgtgggag ttttgatcat aataacgaag tggcaatcga
    21181 ttcagatgag caaatgcatc ttttcttaat tttgcaacgg ataagtaagc taaccgattg
    21241 cttaacactt ggatgagcca ttgactgact acgttgatta aaacgatacc accgaataga
    21301 ataagtaaat ggaacagtcg attgaagcgg acctgtcctt ctccaatcat ttgatcgata
    21361 ctttgaccaa tttgtaacgt catcacaact gttgcaatcc cattgatgat tcctagtatc
    21421 acagctagcc aaatgtcttt tgcataggca gtaaggtaag gaatgaatcg gcggagcgtc
    21481 tgcaaggata atttttttac tttcataact attcttcctc ctgtgatgcg acgatttcct
    21541 gatattctgc tgattgagca agtaattctt catgggtacc ctgagcagca atcttacctt
    21601 gatccatcac taagatatga tgtgcttctt gaatagagcg gacacgttgg gagataataa
    21661 tcactgtact gccaagataa gtctgtagcg ccttacgcaa atccaagtct gtttgataat
    21721 ccaacgcact taaagagtca tctaaaatca gtaatgctgg cttggcaatc aaagctcttg
    21781 caatcgtcag tcgctgtttt tgtccgccag agaaattttt cccattttcc atgacttttg
    21841 tatctaaacc atttggaagc tgacggacaa attcttctgc ctgagcgatt ttcagagctt
    21901 cccaacattc tttttctgtg gcgtctttct taccccattg aaggttttca cgaatagtcc
    21961 cggtaaatag tacagaagtt tggggaacaa gagcaatttg ctgacggaga tgatccagct
    22021 gccaagaacg aacatccatt ccgtccatta taactgtacc ggaactgaca tcatagaaac
    22081 gcggaatcaa ttgtgtcaaa gtcgttttcc cgcttccggt tggaccaatg attccaagga
    22141 ctgtatttcg cttcagctga aatgtaatat tttggagaga aagaccagaa tcaggagtat
    22201 aacggaagtc aacatggtca aaagcgataa ttgcttcaga agaaaaatcg ggagtagtac
    22261 tgacctcagc ttcaagaatc gaattttctg tgtctagcac ctctttcacc cgatttcctg
    22321 aagcttcggc acgtgtgaag atcacgacga ggtttgaaac aacgatcagt gccaaaagca
    22381 tttgattcat ataattgatc aaagcaagaa cttgtccttg ttgcaagcta ccaatattta
    22441 ctttgatccc gccaactgtc agcaaacaga tgatcccgac attcatgatc aaggttgtag
    22501 caggtgataa tagggcagag atattggaaa cacgtaaata attgttcgct agatcatttg
    22561 tgcttttatc gaacttctct gtttcttttt ttgtctgcgc aaatgcgcgg atgacacgaa
    22621 caccgctaag attctgacta acgtgttcat tgagctgatc tagtttttct tgcacttttt
    22681 ggtagagagg aacggttttt ttgatgataa aaaataaaat gattgagaaa ataggcaata
    22741 atgctaagaa aaacagacct acttcccagt cgatcacaaa agccatgacc actgacccga
    22801 tacttaaaaa cggtgctctg atcaataaac gaatgaccat tgctaaagct agctgaagct
    22861 gattgatgtc gtttgtcatt cgggtgatca aggtatctgt tccaaaatga ttcaactcgt
    22921 tgtgagagaa agtattgatt tttttgatta gctgattacg taactctgta ccaaatcctt
    22981 gtgaagctac tgaagcatag tattggcaaa tgatagcaca aatcagacca atcacagaca
    23041 tagcgaccat ccataagccc atttcaatga tttttcctcg gtcattcatt tttagtccat
    23101 tatcgatcaa ggaagccatc attaaaggca ggacaagttc gaagacagct tcaagaaatt
    23161 tgaagaacgg gcccaaaatg atttgcttac gatagtcttt tgcataacgt aataactgaa
    23221 tcatatgatt cctcctatta tttatttgtc cacaattgaa aaaacaagta ctaccacatt
    23281 gtagcttagg aaatttagaa tgaaaagaga acaagccttt tcataaatga aagttttatt
    23341 atgaagaagc tttagaaatt gaatagacta ttttttttaa aaaaaagaaa atgagaaaac
    23401 atgccagtct cttcattcat aggaacgtct attttactta atttttctat aaatgaattc
    23461 tgcataaaaa atatatcaag taaatgttta tagatgaaac tctatttact tcttttttaa
    23521 tttgttcttg caatcttttt tgctacgtaa tttttgtaaa aaagtgggtt gagagaaatc
    23581 taattgattt tcaaagaagg agtatgaaaa tgagaaaaat atttattcgt ttattgtggg
    23641 tactatctta gaggaataaa taatagtgga gaaaactatg gtttggcgca gattctatca
    23701 ggtttgatgg aacgaagaca acctcccgac atcaagtcaa aagccacttg agattttgat
    23761 ctgaaaataa aaaaagaaaa caaggtagga acatcaatgt ttctaccttg ttttcgtaat
    23821 caatgggaaa aaactaagag tggtgtacgt aatcaataaa aaggatggtg acaacagtac
    23881 ctctttactc tctgaaagca tcataactcg tttttcctca ttatgccaat aaattgttgt
    23941 tataccacat ataaggattt gtaatagcta aaaacagaca ggaaagttga aatctgtgaa
    24001 tcttattttg tatggaagga tttatagaac gcaaaaatca agataaagat gaataaaaat
    24061 cctgtttcgg ctatatattg cgtatctacg ccaataaaac gagtttgttc tgtcatagcc
    24121 atataaagca aaggcaaaaa tacaactaca gcgagataag atccgatgat ttctcgaaaa
    24181 gttttccagg aagtatgttt cctgttttta tatttatccg atttcattga atagtcatag
    24241 cctccttatt ctttcatatg ttgaaattcc agtataaaaa ctggaaggaa ctgtacgttt
    24301 tttagaataa aaattataag ggataagtag gctgctgtct ataataagat atatttttca
    24361 attctttatt aaagatttta ttgacaaaat gaatccgttt tctatgatgg aggaggggtg
    24421 aataagttgt tgaagaagga aattttatgt tacttagaga accagacagc ctttttcgat
    24481 ttagaacata tgaacgaggt ctttacagca caaaggttag ctgatcattt tggggtaaaa
    24541 agaaacacag tcagtcatta tttgaaccag ttaaatgatg agcaactact agtcaaaatc
    24601 aattctcgac cagtgattta tttccacaaa gaagcttttg aaaaacaatt ttttaagtta
    24661 cgcacgaatt tttatttttc tatcgatgat ctcaaagagg aacagccttt tttcgcgcaa
    24721 ccaaaagact tattctcttt tatgatcgga tacgacgcca gtttgaaaga aagtatcgaa
    24781 caaatcaaaa cagctcttta ctatccagat ggcggattac cacttttgat tacaggcgaa
    24841 agtggcacag gtaaaagtta tttagttaat ttggtctatc agtattgtct gcttcatgat
    24901 ctgctagaag attctgcacc attcatcact tttaattgtg cacaatatgc agataatcct
    24961 gaacttttga ctagcaattt gtttggacat gttaagggag cgtacactgg tgcagaagaa
    25021 aataaaaaag gggcttttga agcagcagat ggtgggatct tgtttttaga cgaggtacat
    25081 cgcttaacac cagaaggtca ggaaaaactg ttcacctatc ttgatcaagg tgtgatttat
    25141 cgtatgggag aacctaattt gtcacgccgg atcaaaacac gtctcttttt tgctacgact
    25201 gaagagatta ctagtcattt cttaacaact ttcattcgaa gaatacctat ccgaatcgaa
    25261 ctacctgcac ttaatcagcg tagtcgtaat gaacggctag aactgattta ctcttttttt
    25321 ctcgaagaac aaagaaaaat ggggagccct ttgtcagtca gtggaccagc tttgtcactt
    25381 ttaacaagtc agaaattatc cggaaacata ggagagctga aaaatatcgt caaagtttca
    25441 gcagcaaaag catatgctga acagcgtgaa cagcctgaaa tccatgtcac gatccatcat
    25501 ttgcaaaaag aactacttgc gcagcctatt actaaaaata cagcacagca aggaatctat
    25561 attacgcaag accagacctt agagcaatta atggagaagc aacagcccga acagcagcga
    25621 atcgttaaaa gttttgagca aatcctaatt gatttcaaga aaaataactg tctgctatat
    25681 tcttgcgaag gaaaattaaa gcaagaagtg attcaattat ttgatttttt gctgtttgag
    25741 accagtagac aagaaaaaca cgagctgctg gtttattgga cacaatctat ccgagagacc
    25801 tttcgacaaa tggaaactgc ctatcaaatc aaattcgatg gaaacagtgt ctatgccttg
    25861 agttactatc tctatcagcg aagcactatc aaatggttac cggaagaaca tgatattatc
    25921 caactaatca aagaattaga acaacaagtt tctcagtctt atccatctag ttatcactat
    25981 gtccaacgaa ttctggaatt aagtaagatc aaattagata ttgaagacac aagtatggat
    26041 cggattttac tgacgatcta tctaaaaaaa gcgaaatggt caaaagaaaa acgactgcca
    26101 aaagcgatga ttgctgctca cggttatgca actgctagca gtatggcaaa tgtagtaaat
    26161 cgtttattac aagaggatct atttgaatca tttgatatgc cactagatgt cacaccacag
    26221 cagattgctt cagaaatcct tgattattgc gaaaacagcg atgtttcaaa tggattgatc
    26281 atattagtag acatgggatc tctgaaagaa atccatcagt tttttaaaaa acagttatcc
    26341 gtaccgcttt tgattttgaa taatgtgacg acacctttag caatcacagt aggggaatgc
    26401 ctgcaaaaaa atgtatcatt agaagagata gcagaagaga ctgttcgaca gatccagcca
    26461 gaatggcgac tgctctatcc agaggaaaac aaacccaaag cactcattac tacttgcttc
    26521 actggaatcg gtacggcggt ccacttaagt gaactgctag aaaaaagctt acctacggct
    26581 tgtcagttga aaatcatccc atatgaatat cagcaattaa aagataagaa aaacagtgac
    26641 ccattatttt ccatatacga agtcgtagga atgattggca ccacagatcc tgatatcacc
    26701 aatatcgact atctttcatt agaagacctc atctcggggg aaaagatgtc tgtactagct
    26761 gaatggttgg acactactat gaatgcttca gagaaggaaa tattcaatca gcgcatcatt
    26821 cgtaattttt cattggaaaa agtactggat tccgtgacta ttctagatac agagaaggtg
    26881 atgggagaaa tcgatcattt tatgagagaa ttagaggtgc agttgaatcg aagcatcagc
    26941 aatcccaaaa aattagcgtt gtatgtccat gtgagctgtc tcattgaacg attgatccga
    27001 caaatgccaa tcgaaaccta tcaaggatta gagaaatgga agcagtgtca aaaagaaggg
    27061 ttatctgcta ttaaatctgc gtttagtgtc attgaagagc attatagtgt catgatcccc
    27121 aattctgaga ttgcctatat ctatgatatt ttgagtgaag atacagagtt tctctcgata
    27181 gaagaagaat tttgaacata agcaagttgt tgccactaat tttggcatgc aacttgcttt
    27241 tttagtatgt gtaagtgaaa agaggagatg aaaaagtgga tagagaaatt atcttagctt
    27301 cccatggaaa atttgcttca gggatattag attcattaga gctgatctat gggaaaaatc
    27361 attctattac tgtattcgat tgctatactg atgagaattt tgatttagcg gaaacagtaa
    27421 ggcagctttt tatgaggtac aaagataaag aaatcattgt tctgacggat ctttttggcg
    27481 gaagtgtcaa caacgaattc ttacaatata tcaatcggga tcatgtctat ctcgttgccg
    27541 gattgaattt gccactattg attgagctag tatctcggtt ggacgctgaa gtagagacaa
    27601 ctgttttgat ccagcaggtg ctaactgatt caaaaaaaat gatccagttt tgtaacgagc
    27661 ggctcaatca gaaaatagaa gaagacgaat tttaggagga agaaaaatga ttgtattgac
    27721 aagagtcgac catcggttat tacacggaca agtagctttt tcatggacac aaacaatagg
    27781 cgcagactgt attttgattg ctaatgatga tgtacctacg aatgagatac gaaagacaac
    27841 gatcaaatta gccaagccac aaggagtcaa actagtgatc aaatcaattg atgactcgat
    27901 tgccgcgttg aaaagcgggg tgacagataa atataagtta ttcatcgtag tggaatcgat
    27961 cgaggatgca tataagctgg cagaagcgta tccagaaatc aaaaccatca acttaggagg
    28021 gatcaaggca aaacctggta ctcgcagcat cggaaaagcg gtaaatattt taccagaaga
    28081 ggaggaacgc ttgaaacgat taattgacaa acaaatagaa gtagaaatcc gtcaagtacc
    28141 agcagataaa aaagtggatg taagagaagt tttgtaagaa aggaagaacc tcaatgattg
    28201 tacaagcagt cttattagga attgttgcat ttgtcgcaca atcagaatat gcactaggga
    28261 catcgttgct ttctcgtccg attgtgacgg ggctgtttac aggaatcgtg ttaggtgatg
    28321 taaaaactgg gatcattatg ggcgcaactt tagagttagc atttatcgga tcgttctcag
    28381 taggtgcttc gattccacct gatgttgtga ccggtgggat tttagggacg gcttttgcta
    28441 ttacagctgg agccggtaca gaaaccgcgc ttttactagg tttgccgatt gctacgttga
    28501 cattgatatt gaaaaatatt tacttagggc ttttgattcc aattatgaac cataaagcag
    28561 acaattatgc agaagaagga aattataaag gagtcgagca tatgcacttg cttgccggta
    28621 tcggtctttc cttgatgctt ggtgttgtgg taatggtttc ttatatggtg ggaagcaata
    28681 cgatcggaaa tctattaaat atgattcctg acttcgttca aaaggggttg tcagtagcta
    28741 cagggttgat tccagctctt ggttttgcga tgcttgctcg tttgctgatc aacaaacaag
    28801 ttgcaccata ttttttctta ggttttgcta tagcttctta cttagagatt cctgtgaccg
    28861 gagtagctat ttttggtgcg attttagcag ttgtggtagt caatattatg aatgtccgtc
    28921 aactaccact tcaaacaact gcaggggagg tagaagaaga tgaagaattc tgagatcaaa
    28981 aacagcaaaa taacgaaaaa agagctaaat cacgtatttt ggcgttcttt tcaaatggag
    29041 ttttcttgga attatgaacg tcagatgaat atggcttatg tttatgcaat gattccgatt
    29101 ttgaaaaagt tatatcagtc gaaagaagaa atggcagcag ctttgaaacg gcatctagag
    29161 ttttttaaca cgaccccaca tatcgttaca ctgattttag gaatcacggc tgcaatggag
    29221 gaagaaaata aggatgatcc tgaatttgat gtgactgcga tcgatagtat caaaacatct
    29281 ttgatgggac ctcttgcagg gttaggagat tctttctttt ggggaacttt gcgattgatt
    29341 gccacaggtg tgggtacctc tttagccatg caaggaaata ttttaggccc tatcttgttc
    29401 attttgattt tcaatattcc gcacattctg tttcgctatc tagcaacagg ttggggatat
    29461 cgcttaggaa cgggtttttt gaaaaagatc caggcaaacg gtatgatgga aagtttgaca
    29521 ttaggcgcgt ctattattgg actgatggtc gttggagcaa tgactgccac gatgatcacc
    29581 atcaatattc ctttaaaaat cggttcagga gaaaatgctg tgactgttca aagtattttt
    29641 gatgatatcg ttccaaatat cttatgtctt ggagcatttg gcatagtgtt ttatctattg
    29701 aagaaagaag tgaaaccatt aacgatcttg atcggactag ctttctttgg aatttttggt
    29761 tcgtggatag ggatttttta aaaaaggagc agagaaaatg tttacgatgc aagattatat
    29821 ctatgaagaa aaggaagtac taagtacgat cttgaaaaag aatgattttt cgacaagaga
    29881 acatatgaaa aaaacagtga atttactgat cttagcaacg ggttcatctt ataacgcttg
    29941 tttagcagcg aagccagcat tggaatctta tggtgatctt actgtcgata ttcaagaacc
    30001 tttttatttt ttcaattatg gaaaactttc tccttcaatc gatactgtga ttgctgtgtc
    30061 acaaagcggg aaaagtgctt ctactgttga agcagtaaaa atgatacaaa aacaagggtt
    30121 acctgttgtt gctatcacaa atgacgtgca aagtccactt gctttagaag ctgcacaaat
    30181 tattgattta ggtgccgggg tagaatcagt aggcttcgtt acaaaaggct attctgcgac
    30241 tgttttacag ttactactct tagggatagg aattggaata agtaaaaata aaatcagcaa
    30301 aaaaatagaa caagactaca tgcaacagtt gcgcaaaata atcaaccatt taccagcgat
    30361 tattcaaaaa acggaagaat tttttgatgg atatcaaagt ttatttcgtt tagcacagcg
    30421 tttcgtcacc attggatatg gtccaaactg gggaacagct aaagaagcag agacgaaatt
    30481 gactgaaaca atccgcgttc cgtcacaagg atttgagtta gaagcttata tgcacggccc
    30541 atacctggag gcagatgctt cgcatttgct attctttatt gaaggggaaa gtgtaaataa
    30601 ggaacgttcg caaaagttgc aaagatatat gagtcggtac gttggagaga ctctcaccat
    30661 taccacaaaa aaagcaagaa acgagaaaac acttggatta gcaatagaat gtgatgaata
    30721 tctgtctgta ttagcgcttg tcgttccgtt tcaactattc gcttataaaa ccgcagtagc
    30781 taaaggtatt gacttgaaca aaaagatttt tgaagacttt gatacagtct tgaaaagtaa
    30841 attataagag gtggaaatca tgctgaaatt caatgaagaa gaacaaatca aagcaataaa
    30901 aggcgcacta gctttacgtc cgcaagtaga agaaatcatc gataaacttt atgcggaaaa
    30961 atttgacgct gtctattatc taggaatcgg tggaacgtat gcttcctcga tgcaagctgt
    31021 cacttatatg aatgggaaaa gcaacttgcc tgtctttgta cagcatgctg cagaatatta
    31081 cactactgga aacaaaagat taacaaaaga ttctatcgtt attttgtcct ctgttaccgg
    31141 tacaactcaa gaagtagtga aagcagtaga acaaatcaag aaagtaggag cgacactgat
    31201 tggatttatc gataaagcga acagtaagtt gtctcagcta tgcgattttg tggtcactta
    31261 tccagctcct ggaacagaac aaatcaaatt ttttatggca gcagatcgat tgatgtattt
    31321 gaatggagaa ttcgaagctt attcagaata ctatgaacag ctcgaacgtt atttaccaac
    31381 agggttagtg gaagcagaga aaaaagcaga tgcatttggc ttatcatttg cagaaaaaca
    31441 tcgacgtgat agtatgcatt attttattgg agcagggaat caatggggag ctgtttattc
    31501 atatgccatg tgttactggg aagaacaaag ctggcttcca tcgaaatcta tccatgctgc
    31561 cgagtttctt cacgggacgt tagagatcgt agaagaaaca accccagtca cattattttt
    31621 aggcgaagat gaacagagag tgttatctga acgtgtggct aaacttttgc caaaaatttg
    31681 cagcaattat acattgatag acacaaaaga ctatccagta gaaggtatca gtgaaaaata
    31741 tcgcggacgt gttttgtctt tcttattgat gcatgtagtg acacaacgca tcgatgctca
    31801 tgtggagcag ttgaactgcc atccattaga gatacgccgc tactatcgtc agtttgatta
    31861 ttaggataga ctggataaaa agaatataaa aaataaagag agcagcgata actgctccct
    31921 ttattttttt aaaatattgg catcaattac gttataaaac acattttttg cattatctac
    31981 agttcaactt ataatctgtt taatgggaca tgtgggtgcc aaccattttt tgttatgcaa
    32041 tagtactatt tagttgttgc atggatccgt agtaaaagtc caagtacaac gagttgcgcc
    32101 agtttacata gcgttttgta tccagaatgt tgactaaaac aaaagttcct atgtcaaccg
    32161 tcaacagaga caatctgtcc gttgcaggac ctgacagatt tagaaatgtt caatctgtct
    32221 cttatgtatt ttaaatcaat actcactagg agaagtttcc taacctccta atagtaatgc
    32281 tggtagtaac attgtcaaaa ctgcttcctc ttaggaacaa gcaaagcata acgagtgtaa
    32341 aaataaaatg tagactattg taacatatca ttgattattt caagtaaaaa cgataaaaga
    32401 agtaaacaaa cacttaaaaa caatggaaat tagtcccaac tttaagaaat gtatactata
    32461 atggaaatta tcaattattt atttggagga atatccatga aacaaaaaat cgcagttctt
    32521 ggtcctggat cttggggcac tgctttagca caaacattgg cagaaaacgg gcatgatgtc
    32581 cgtatttggg gaaatgtacc tgaacagatt gatgaaatca ataagtatca tacaaatcag
    32641 cattttttac ctgatttgac gatcccagaa tcgattatcg gatataaaga attggcagaa
    32701 gcagtggata acgcagatgc tattttattt gttgttccaa caaaagcgat tcgttcagtc
    32761 gcacaggaac ttattggtaa aatgaatact aagccaatca ttatacatgc aagtaaagga
    32821 ctagaacaag atacgcataa acgaatctca gaagttttgg ctgaagaaat tccagaagaa
    32881 aaaagacagg caattgttgt attatctggt cctagccatg ctgaggaagt ggcagtacat
    32941 gatatcacaa caattacagc tgcaagtatt gatcaaaaag cagcagccta tgtacaaaaa
    33001 ctgttcatga atgattattt caggatttat acaaataatg atgtgattgg cgtagaaact
    33061 ggtgcagcac tgaaaaatat cattgcaatt ggtgccggcg ctatccatgg attaggattc
    33121 ggagatgatg caaaagcagc aatcatgact cgaggattgg cagaaatcag ccgtcttggt
    33181 gtcgcgatgg gtgccaatcc tctgacattt atcggtttga gtggtgtggg ggacttgatc
    33241 gttacttgta caagtgttca ttctcgcaat tggcgtgccg gtaacttgtt aggacaggga
    33301 cataagctgg aagaagttct agaaaacatg ggaatggtcg ttgagggtgt ggcaacgaca
    33361 aaagcagcag tagagctagc tcaacagcta ggtgttgaga tgccaattac tcaaacaatc
    33421 tataatgttt tgtataatgg agaagatatc aaaaaagcag caaaagaaat catgttacgt
    33481 gacggaaaaa tggagaatga attttctctt cgttaaatag atgatcaata gttaatcgtc
    33541 gtttaggagg aacagcaagt gaaggtcaaa aaagcagtca ttcctgccgc tgggctagga
    33601 acacgttttt tgccggcaac aaaagccatg gcaaaagaaa tgcttccaat cgtggataaa
    33661 ccaacgatcc agtttattgt ggaagaagca ttggattcgg gaatcgaaga catcttgatc
    33721 gtaacgggaa aagaaaaaag acccattgaa gatcactttg atgcaaatat cgaattggaa
    33781 aacaatttgc gggaaaagga aaaaacagaa ttactcgctt tagttgaaga aacgactcaa
    33841 gtcaatcttc acttcatccg tcaatctcat ccaagaggac tgggggatgc cgtgcttcaa
    33901 gcaaaagcat ttataggaaa tgaaccgttt gtcgtcatgc tgggggatga tttgatgaaa
    33961 gatgatatcc cattgactca acaattgatg atggattatg aagaaacaca agcttccgct
    34021 gttgccgtca tgcgtgtacc agaaaatgaa acatctaaat acggaattat tgatcctaaa
    34081 acagatagcg aaaagggtcg ttgcagagta aaagggtttg tagaaaaacc tgaaatggga
    34141 aaagcaccaa gtaatttagc cattatcgga cgttatctac tgactcctaa aatatttgag
    34201 atattagaaa ctcaagaacc aggagcagga aacgagatac agctaacgga tgcacttcag
    34261 actcttaacc agacagaggc ggtctatgcg agagagttta aaggaaaaag atacgacgta
    34321 ggcgataagt taggatatat gaagacaaat attgaatatg gacttcaaca ttcggaaatt
    34381 gggacttcat taagtgcata tattatccag ttgagtaaaa agctaagaac agaaagttaa
    34441 ctggaaataa gtggcctgct gagaatgaaa ggctatttgt tattaaagcg gcagcttgtg
    34501 cgtcatttgc gcaactgtcg ttttagtttt ttgactctag atcagttgag tcgtggaaaa
    34561 cgaaagatag aaaaagactt tcgtgaatag attcagaaaa tattatgcta atggtttaat
    34621 ttttttttaa gttgtataaa agaaaatgat tacataagag aaatgaaagc caagccagaa
    34681 gattcatagt agaataaaaa ggcgaatttt ttaattataa aaaaaggtgt gtgttcacta
    34741 tgggaaaatt agtcgctgtt gcacagtcgc tgcttgatat actcaagtgg ctgatggaag
    34801 gaagcaattg gatatttgtg ttgccactat tgcttattag tctatttatc atgtttcgca
    34861 tggtcagaaa aaaagaaacg tttctgattt ttatctggta tgcagcatcc gttggggttg
    34921 ctaccttgat ggttgaattg gcgttaggta gttgggaagc ttgcgtattt gctttatttt
    34981 ttgctgtact gtatctaatc atttatatcc catatatgaa aagaaagtct cggataggca
    35041 gaaaaggtag acatacaac
//
LOCUS       DANKKG010000016        34959 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000016 DANKKG010000000
VERSION     DANKKG010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.016"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            156..1862
                     /gene="efbA"
                     /locus_tag="PW004_000651"
     CDS             156..1862
                     /gene="efbA"
                     /locus_tag="PW004_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HDL2725645.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2020..2922)
                     /locus_tag="PW004_000652"
     CDS             complement(2020..2922)
                     /locus_tag="PW004_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL2725646.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2919..3611)
                     /locus_tag="PW004_000653"
     CDS             complement(2919..3611)
                     /locus_tag="PW004_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HDL2725647.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3654..4295)
                     /locus_tag="PW004_000654"
     CDS             complement(3654..4295)
                     /locus_tag="PW004_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL2725648.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4333..4647)
                     /locus_tag="PW004_000655"
     CDS             complement(4333..4647)
                     /locus_tag="PW004_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725649.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4635..5987)
                     /locus_tag="PW004_000656"
                     /pseudo
     CDS             complement(4635..5987)
                     /locus_tag="PW004_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372436.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
     gene            complement(5984..6916)
                     /locus_tag="PW004_000657"
     CDS             complement(5984..6916)
                     /locus_tag="PW004_000657"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HDL2725650.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6913..7689)
                     /locus_tag="PW004_000658"
     CDS             complement(6913..7689)
                     /locus_tag="PW004_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HDL2725651.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7743..10928)
                     /gene="carB"
                     /locus_tag="PW004_000659"
     CDS             complement(7743..10928)
                     /gene="carB"
                     /locus_tag="PW004_000659"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HDL2725652.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10933..12009)
                     /gene="carA"
                     /locus_tag="PW004_000660"
     CDS             complement(10933..12009)
                     /gene="carA"
                     /locus_tag="PW004_000660"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDL2725653.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12014..13294)
                     /locus_tag="PW004_000661"
     CDS             complement(12014..13294)
                     /locus_tag="PW004_000661"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HDL2725654.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13313..14239)
                     /locus_tag="PW004_000662"
     CDS             complement(13313..14239)
                     /locus_tag="PW004_000662"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HDL2725655.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14252..15535)
                     /locus_tag="PW004_000663"
     CDS             complement(14252..15535)
                     /locus_tag="PW004_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HDL2725656.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="PW004_000664"
     CDS             complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="PW004_000664"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HDL2725657.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16323..17234)
                     /locus_tag="PW004_000665"
     CDS             complement(16323..17234)
                     /locus_tag="PW004_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDL2725658.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17231..17695)
                     /locus_tag="PW004_000666"
     CDS             complement(17231..17695)
                     /locus_tag="PW004_000666"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDL2725659.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17700..18398)
                     /locus_tag="PW004_000667"
     CDS             complement(17700..18398)
                     /locus_tag="PW004_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDL2725660.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18385..18771)
                     /locus_tag="PW004_000668"
     CDS             complement(18385..18771)
                     /locus_tag="PW004_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725661.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18918..20588)
                     /locus_tag="PW004_000669"
     CDS             complement(18918..20588)
                     /locus_tag="PW004_000669"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HDL2725662.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20771..21004
                     /locus_tag="PW004_000670"
     CDS             20771..21004
                     /locus_tag="PW004_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HDL2725663.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21103..21534)
                     /locus_tag="PW004_000671"
     CDS             complement(21103..21534)
                     /locus_tag="PW004_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HDL2725664.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21613..22026
                     /locus_tag="PW004_000672"
     CDS             21613..22026
                     /locus_tag="PW004_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HDL2725665.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(22064..23212)
                     /locus_tag="PW004_000673"
     CDS             complement(22064..23212)
                     /locus_tag="PW004_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDL2725666.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(23334..23930)
                     /locus_tag="PW004_000674"
     CDS             complement(23334..23930)
                     /locus_tag="PW004_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL2725667.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(24130..26772)
                     /gene="alaS"
                     /locus_tag="PW004_000675"
     CDS             complement(24130..26772)
                     /gene="alaS"
                     /locus_tag="PW004_000675"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HDL2725668.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            27160..27591
                     /locus_tag="PW004_000676"
     CDS             27160..27591
                     /locus_tag="PW004_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2725669.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(27636..28985)
                     /locus_tag="PW004_000677"
     CDS             complement(27636..28985)
                     /locus_tag="PW004_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDL2725670.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(29055..30218)
                     /locus_tag="PW004_000678"
     CDS             complement(29055..30218)
                     /locus_tag="PW004_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HDL2725671.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            30347..30670
                     /locus_tag="PW004_000679"
     CDS             30347..30670
                     /locus_tag="PW004_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725672.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(30853..32202)
                     /locus_tag="PW004_000680"
     CDS             complement(30853..32202)
                     /locus_tag="PW004_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2725673.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(32271..33224)
                     /locus_tag="PW004_000681"
     CDS             complement(32271..33224)
                     /locus_tag="PW004_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HDL2725674.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(33326..34516)
                     /locus_tag="PW004_000682"
     CDS             complement(33326..34516)
                     /locus_tag="PW004_000682"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HDL2725675.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
BASE COUNT         9950 a         7642 c         6120 g        11247 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaaattctg tcacaacccc tcttttttga
     1981 tcaacggtgt tcaataaaca gcgttctcat ctgcttttct tagctctgct ggtccagtaa
     2041 ccgagtaagc tctttgataa atctttgagc gatttcactg agcggacgtt tcgccgaata
     2101 aatgatataa aattcattta gttcgccttc tgccaaaggg attaaccgca agtgctcgcg
     2161 aatccctcca atcacaatac cagatcctga agcgtaagca tcagtttcaa gaacgatatt
     2221 catcaaagta cctcgatcac tcgtataaat cactgtttct tgatcaggga tctcgataag
     2281 gtcttcataa aaatacggga aattatttcc atcctgtgta aaacgaactt gaggataaga
     2341 taacaattct tctttggtga tctctgcgtg ttcagctaat ggatgtcctt gccttaaaaa
     2401 aatatgcgtt ttgaattttc caatgctgcg gccacttagc tctccttgtt gtaaatatct
     2461 tctgatacca gcttgattat gctcattcat ataaataatg ccgatctcac tgtgctgctc
     2521 tttgacttct tcgatcactc tcgctgttgt cgtctccatg atccgaaatt gtttgatctc
     2581 gtctttgaat tcctcgagta gctgtgcggt caaaggacct aagaaatcat aatgctgggc
     2641 agcgatcgaa aaattgtctc tcttttcact cgttagatag tgagtttcta atgtatcaag
     2701 ctggaccaat attttctcgg cacgatttaa gaagtcttgc ccttcttctg ttaaatatgc
     2761 ccctcgattc gttcgagtaa acagcgtgat ccctaactcg gtttctaatt ctttgatacc
     2821 agctgaaaga cttggctggg agacaaacat tttctttgcc gcttctctga aactaccatt
     2881 gtttgctaca gcgaccacgt atttcatctg ctgaatattc attgctcaaa ctcctcgaaa
     2941 taataaattt ctcttccatt caagggctgg attggtcggt tgttcggtcg agcatactga
     3001 ccatcgaagg ggagaagaaa ctcttggttc agttttccta catgatccat tacaaaccat
     3061 tggattggcc cgctggtagg cggagttgtc aatgagccaa tataatggaa atggcttttt
     3121 tgctccggaa agaacacatc cggatttacc caatgctcat gtttttctaa gttccaactt
     3181 tccccatttt cttgattata cagccaacct tcctgtacca ttagctggta catgacaccc
     3241 acaactagat tctctttttt gctgttcata tgaacaaaat gaaattcaat atcttcctgt
     3301 tttccgttga tgatatgttc agaaggcaag tggaaatgaa tatctgtcaa ataatagcgt
     3361 tctttcttga actctacata actgagttgg tcaaaaggta ctaaatgaat cgtgtttttg
     3421 aattccttgt cagtaaaccg ttgcttttga taatgaaagg aaagcgtgtc tccttctatt
     3481 ttggtttctt cccccttggt caaatgaata ggcgactgca atggaaattc cgcacctctc
     3541 gcaaaccaat cacataaact cgcccaattt tccggacctt tgtctccaga atagccccat
     3601 tcgacatcca tattcttttt caactatact tccccctgca tctcatatca tttttatttt
     3661 tcttctttct ttacaatcgt tgagacggtt tctaaaaacg cctcttttcc ataaaattcc
     3721 tggttcaata gttccggaaa tatctcttta aagaaatact gaacagaagg ccgatgttca
     3781 aagcttttaa tcgtagtcac tgcctcaatg aacatctcct tataaacttt ttccgggtta
     3841 tttttgatga tttcatcaaa agattcataa agaaggtcaa ccttatctga aatggctaat
     3901 agttctcctt ctacggtttc atcttttcct tcgaacaacc gacggcgata aacttcttgg
     3961 aactcagctg gaatctcttg gttgatgaat tcgtccgtca tcttttcttc aactgtttgc
     4021 agcatccctc ttaactcttt gcttgcgtat ttcaccggcg ttttgatatc accaataaaa
     4081 cgctctgtgt agtcatgatt caatgatttt tcatagagca gtttccaatc gatcggttgt
     4141 ccattcgctt cttcaatgtc tcctagaact tgcgcaatag atgctacacg atacgaatgt
     4201 gctgcaactg tatgctctgt atatttaaaa aatccaggtg cccttgtgat cgtctctaaa
     4261 ttgttcaatc ctaaaataaa ttcattcaac cccataaaag ttacctccct ttgagatttc
     4321 tttttccgaa tatcatatca ttaaataact gatattcgca acatttccgc tctttaccat
     4381 ttttgaaaca gctcccttac caaaaaatag agaaaatcta tatataatag aaaaaagaga
     4441 agagattatt ctcttctttc tgctaaccag tcttagaaaa atgaatttta aaaaaatcaa
     4501 aaactgatca cgatagaagt ttcttttcat acctgatgaa aagatgcttc tcgcaatcag
     4561 tttttggatt tttgctattc caattgatga ttctggagac acaaatcgga tttctctatc
     4621 ttcatcaagc gcgcttaaat ctgccaattt tcaggatctt ttttccattc ttttagcaga
     4681 gttaattctt gctcatcgat ataacgatct tctaaagctg cttcaatcaa tacagaataa
     4741 ttggtcaacg tcataagagg gatttcggct ttttcgaaat ttgcttttcc ttttggcaac
     4801 tcatacgtaa agatagccgc aacaccaagg atatccgcac cttctctttt tgctgcttca
     4861 gctgcttcca agacacttcc gcctgtagaa atcaaatcct cgatcacaac catcttctga
     4921 ccttcaaata tacgtccttc gatttggtta cctttcccat gatctttcgc cttacttcga
     4981 atatagacca ttggcaaatc cagtatttcc gctacccatg cggcatgagg gattcctgct
     5041 gttgcagttc cagcaatcac ttgtacttca gggtaagctt ctttgatttt gcttgctaat
     5101 ccttttgcga tctcttttcg gacttttgga tagctcatcg tgattcggtt atcacagtaa
     5161 attgggcttt tgattccact tgcccatgta aaaggattac ttggatttaa aaatactgct
     5221 tcaatttcta ataagtcttt tgcgatactt tgttcaacat tcattttagg ttagctccat
     5281 tctgtttgaa tcgtttgata tgctttataa ggattagttg actgagtgat tggtcgacct
     5341 ataacgataa aagttgagcc gatctttttt gcctgagaag gtgtgactac tcttgtctga
     5401 tctcctgcga ctgttccttc tggtcttata cccggagtta agcaaacaaa ttcgtctcct
     5461 gccatttctt tgatcgcttc tacttcccaa gcagaagaaa ccacaccatc taatcctgct
     5521 tttttggcac agctagcata atggatcaca cttttttcta aagaaacttc gattagttga
     5581 tcggcatgca tttcttcttc acttgtagaa gtaagctgag tgatagctag aagtagcgga
     5641 cgttttccac cttctggagt cccctcttct aatcctcgca ttgccgcctc catcatacga
     5701 ataccgccag cggcatgaac acaggtgaga tcaacaccaa gctttgccaa tccccgcatt
     5761 gctttttcga ccgtattcgg aatatcgtga agtttcagat ctaaaaatac attatgacca
     5821 gcctctttta gataacgtac gatttcaggt ccttcttggt agaacaattc cattccgatt
     5881 ttcacaaaca atttttcttc tttcgggaaa aatcgaagaa agtcttctac ctcttgccta
     5941 gttgaaaaat ctaatgcaat aattggtcgt tggtctagtt gattcatcgt tcacgttcct
     6001 ctctcacttc tttgattaat ctttccagcg attcgatgcc tagttcgctc attcgcacag
     6061 ggagctcttt aattagttta ggacagatat acggatcggt gaaattcgcg gtccctatag
     6121 ctacagcact tgctccagcc ataaacatct caagaacatc atccactgtc tgcacgccgc
     6181 ccatcccaat gattggaagg ctggatatac ttgctacttg gtgaatcaaa cgaatagcga
     6241 caggcttgat tgctggtccg gagagtccac ctgtttgatt ggctaaaatc ggttttcttg
     6301 tcttaaggtc aatccgcata ccaagaagag tattgatcat cgtaaaccca tctgcccctc
     6361 cagcttcgat tgcctgagcg attggaacga tgtctgttac atttggggac aatttgacat
     6421 agacaggaac agaagaaact ttctttactg cttgtgttaa ttgaaaagca acatcagaat
     6481 ctgttccaaa agcaattccg ccatgtttga catttgggca agaaatgttc agctcgattg
     6541 ctttcacatt tggtgcttcg ccaattttgc tacaaacttc tacatagtct tcttcacaag
     6601 caccagcaac atttgcgatg atgggtaact cattgtactc ctctaattct ggaagaagtt
     6661 tatgcataac aacgtctaga ccaggatttt gcaaaccaat tgcattcaac atgccagatg
     6721 gtgtttccgc cactcttggc gtgggattgc caaagcgggc atttggagtc gttgccttga
     6781 ccatgatcga tccaagctga ttcaaatcat agtattttcc gtattctttg ccaaaaccga
     6841 aacagccgct tgccggcatg ataggatttt ttaattctag gccaggaagt ttgaccgcta
     6901 aaggattctc attcatataa ttacctctcc tattttaaaa atcggtcctt cgtcacaaac
     6961 tttcacactt tttgttcctg tttcatcttc cggcgtatga caaacgcaag catagcaagc
     7021 ccctatccca catgccatgc gggattctaa tgataactgg acattttgat gcgaagaaaa
     7081 cagctgttcc actgtcttca ataacccgtt tgaaccacat gcaaaaacag ctgtaggttc
     7141 cgtcttcgtt cctaacaata aattaccgac atttccgtgc ataccgtatg ttccatcgtc
     7201 agtagcgatt cttgtttctc ctagacgatt aaattctttt tcataataaa cgacttcatg
     7261 tgtggcaaaa cctaaaaaat gcgttggctt aattcctttt tcgactaact gttttgataa
     7321 ctcatataga ggagggacac cgattcctcc accgattata aatacttcgt ctcctgtatg
     7381 aagattggac aaatcaaaac cattgcctaa aggacccatg acatctaaat ggtctccggc
     7441 agaaagagta gagaagactc tagtcccctc accttccacc cgataaatga tacggcaagt
     7501 atttgtttcg tggtcgatat gattcaagct aatcggtcgg cgtaatagca agtctgcacg
     7561 cggcacgaga atatgcaaga actgtcctgg ttgctgcatt tcttttacta gatcaccttt
     7621 caatgtcatt tcaaaaatct ttggtgccag ctgtttttgc tgaacgattt ccatcatttc
     7681 ttgtttcatc tgtcccctcc tgcaacggtt tttcgtcttt ttgtcttaac atgtgctcgc
     7741 cgttaaatag ggttagttga aaaagcacgg gattccatca ctcgaacgat cgcatctgct
     7801 gtgtctaaag aagtaaacaa tggaatacca tgttcaactg cttctcttcg gatgatgaaa
     7861 ccgtctttag aagcatttcc ccgatcttta tcaatcgtat tgacaaccac ttgtgtttta
     7921 ccatctctta ttaagtcgac tacatttttt tctgctgctt cctcagaaat tttcgctact
     7981 gtttcaacga ccagaccatt tttttcgaaa taagcagctg tatgtttcgt tcctatcaga
     8041 ctatagccaa tttctgtaaa acgtttagct aattctagtg cttcttcttt tgtttcgtcc
     8101 gcaatcgtaa ataatacggc tccatattca ggcaagtgta atccagaagc ttcgaatgcc
     8161 ttgtacaaag ctttgtctaa ttgtctgtca gatcccatta cttctccagt cgatttcatt
     8221 tcaggcccta agtaagtatc aactttctgc aatttcgtaa aagaaaagac aggtgcttta
     8281 acatgaactt cttttgtttc tggataaagt ccgttttcat aaccaagtgt ctttagtttc
     8341 tctcccaaaa tagctttcgt cgcaatttgt gccattggaa ttcctgtcac cttacttaag
     8401 aatggaacag tccgacttgc tcgaggattg acttcgatca cataaacctc attttcatgg
     8461 atgacaaact ggatattcat catcccgaca caattcaaac ctaatgctaa gcggatcgtg
     8521 taatcttcga tcgttttctt gatcgattca gataatgttt gaggtggata cacagccatt
     8581 gaatcccctg aatggacacc agcacgctca atatgctcca tgatccctgg gatgagtact
     8641 gtttcaccgt cacaaatcgc atccacttcg cattcaccac caagcaagta acggtccact
     8701 agtactggat gttctggaga tgctttcaca gcatgttcca tgtaatcttc taaatctcgc
     8761 tggttttcaa cgatttccat cgcacgtccg cctaatacat agcttggacg aacaagaact
     8821 ggataaccaa ttctcgaagc gataaccacg gcttcttctt tacttgtagc agtatcccct
     8881 aaaggttgag gaatatccaa ctctttcagc gcttgctcga ataaatcacg attttccgca
     8941 cgatccaagt cttcgatcgt tgtccctaag atttttacac cagcagcagt caaaggttcg
     9001 gctaagttga ttgctgtctg accaccaaat tgaacgatta ctcctatcgg ctgttctagc
     9061 tcgatcacat tcatcacatc ttcgaaggtc aatggctcaa aatataattt atccgaaata
     9121 gaaaagtcag tcgaaactgt ttcaggattg ctgttcatga tgattgcttc gtatcctgcc
     9181 tgctggattg ctttgacaga atgcacagtc gcatagtcaa attcaacccc ttgtccgata
     9241 cggataggac cagatcccag tactagaatt gagggctttt ctgatcgtga actttcattt
     9301 tcatattcat aggaactata aaaataaggt gtttttgatt cgaattccgc tgcacaagtg
     9361 tcaaccatct tatatacagg aatcaaatca ttttcttttc tcatctggcg aacggcttct
     9421 tcgtttattt cccacagctc cgccactttt ttgtctgtaa aaccgttttg tttcgctgtt
     9481 ttcaatatct tgtcatccaa cggctgctgt ttcaactgct gttcgatttc tacgatatgc
     9541 aacagtttat ctaaaaagaa taaatcgatt tttgtcaaat ccgctaattc ttctatggtg
     9601 taccctcttc taatagcttc agctacataa aacaggcgat catcttgtgc tcgaaccact
     9661 ttttggatta ggtcatcgtc cgtgatgtct gccaactcag gaagttcgat atggtgtgta
     9721 ccgatttcta aagaacgcac agctttcaat aaagactctt cgatattccg accgatagcc
     9781 atcacttcac cagttgcttt catctgcgtg cctaaaagcc gttctccgtt ttcaaactta
     9841 tcaaacggcc agcgaggaat tttagctaca acataatcta atgcaggttc aaattctgca
     9901 aaagtcgttc ctgtaacagg atttttcatt tcgtctaatg ttaatccaag cgctatttta
     9961 gcagctagct tagcgatcgg atagccagtc gccttacttg ctaacgcaga tgaacgagaa
    10021 acacgcggat tgacttcaat cacataataa ttaaaactat gtggatcaag agcgagttgg
    10081 acattacagc ctccctcgat cttaagtgct cgaataatcg aaagagaagc atcacgaagc
    10141 atttgatatt catggtcaga caatgtttga ctaggggcaa atacgataga atcacctgta
    10201 tggataccca ccgggtcaaa attttccata ttgcagacaa cgattgcatt atcagccgaa
    10261 tctctcatca cttcatattc aatttcttta aaacctgcaa tacttcgttc aatcaaacat
    10321 tgggtagcgg gggaaagact taatccattt tctgatacga tacgtaattc ttcttcattt
    10381 tcacacatac ctccaccagt tccgcctaaa gtaaaggctg gacgaacgat gacaggatag
    10441 ccgattttat ttgcaaaatc tacagcttct tctactgtat tgacgatttc agactcgggg
    10501 attggctgat tcaattcttt catcaactgt ttgaatagat cacgatcttc tgcttgatcg
    10561 atcgcagaaa gctttgttcc tagaagttct atgttcaatt cttctaaaat acctgatgct
    10621 gccagctcca ttgccatatt gaggcctgtc tgtccgccta aagttggaag gagagcatct
    10681 ggacgctctt ttcgaagaat ccttgataca aattcaagtg tgatcggttc gatatatact
    10741 tgatctgcca cttctttatc cgtcatgatc gtcgcaggat tagaatttac taaaaccact
    10801 tcatatcctt cttcttttaa ggcaaggcag gcttgtgttc ctgcgtagtc aaattctgcg
    10861 gcttgaccaa tgatgatcgg accagatcca atcaccataa ttttcttgat atctgtacgt
    10921 ttcggcatct gtttactgct cctttcctgc gtccatcatt tccataaatt catcaaataa
    10981 atgtagtgca tcatgcggac caggggcagc atctggatga aactgcacac tgaaagcggg
    11041 ataatctctg tgtttgactc cttctactgt tccgtcattt acttctacat gagttacgat
    11101 gagtttttct ggatcaatcg tggcttcatc gactgcatag ccatgatttt gagaagtaaa
    11161 atcaattctt ccggtcgcta cttctcgtac cgggtgattt aatcctcgat gtccgaattt
    11221 cattttatag gtatctgcac cgttggctaa agcaaataac tggtgaccga gacaaatacc
    11281 aaaaatcggt acttttcctt ggattttttt gatcatttcg attgcttctg gcacatcttt
    11341 tggatcacca ggtccattcg tcagcatgac tccgtctggt gatagcgcta aaatcgtctc
    11401 ggcatctgtg ttatagggaa gtactgtgag gttacagttt cttttactca attctcgtaa
    11461 aatactatgt ttcaagccga agtcaatgac aatgactttc cgacctgttc ctggacttgg
    11521 ataaggtttg ttagttgaga cttgctgcac ttgattggtt gctagaacgg ttgctttcag
    11581 ctgatcaaat gcatgttcga aggaatctcc agcatcaact atactagctt tcatcgttcc
    11641 gacttcacgg attttgcgag taagggcgcg cgtatcgata cctgatattc caggaattcc
    11701 tttttgtttc aaaaatgaat ctagcgtcat ttggtttcgc cagtttgaag ctacacgtgc
    11761 atgttctttt actactactc ctttgcatgt tggagaaatc gattcataat catctctatt
    11821 taccccataa ttacccacca taggataggt aaatgtaatg atttgaccgt taaaactttg
    11881 atcagtgata gtttcttgat aaccagtcat acttgttgta aaaacgattt ctccgaagac
    11941 actagcatct gcaccaaaag cctttccttc gaaaactgtt ccatcttcta atataagcaa
    12001 acgttccata cttttattct cccttctgcc acacaagttt tccgttgaca aaagtcattt
    12061 gtgtttcgcc ttttactttc cagccgataa aaggtgtatt ctcccctttt gataaaaagt
    12121 cttctttatc gatcgtgcaa gtttgtgtaa tatcaaaaac tgctacatca gcgggggcac
    12181 caaccgttag cgttcctgca ttcaaaccaa aaatctcagc cggtttaact gccaaccaat
    12241 tgatcacttg ttccaacgtg aatcgaccag tttctacaaa atgagtatag atcagttgaa
    12301 acgctgtttc gcttcctacg ataccaaatg gtgatttcat gaaactttgg cttttttctt
    12361 ctaatccatg gggggcatgg tctgtagcga tgcaatcgat cgtaccgtca agcaagcctt
    12421 cgatcaaagc ttctctgtcc tcccgtcctc ttagcggcgg gttcattttc caaaagccga
    12481 aatcttcggg aatatcttcg tcgatcaaaa taagatgatg gggtgaaact tctgctgtta
    12541 cgtggattcc tgcttttttt gcgtctctga ttacacggac actttcttta gtagaaacat
    12601 ggcaaacatg gtaatgaaca ccagttgctt cagctagcaa cagatctcta gcaatttgag
    12661 aagactcggt tacgcttaag atcccgggaa gaccaagttc ttctgccttc ttgccagcgt
    12721 gcataacgcc gccaaataat aaagattcat cttctgtatg ggctactagg gctttattgt
    12781 tttttgcagc ttctttcata gccagataca tcgtcccagc tgtttggaca cctacaccgt
    12841 catttgtgaa agcaaaagca ccttcttcaa tcagtgcttc ttgatcaact aatttctcac
    12901 ttcgcagatt ttctgtgatt ggcgcatact gcaatacctt cacaactgca tcttttctaa
    12961 tgatgtcata tacttgtctc agcttttctg ccgtatcagg tacaggattt aaattcggca
    13021 tcgcgcatac agtagtgaaa ccgccacgag cagctgagcg agtcccagct tcgatcgtct
    13081 ccttgtatgt aaatcctggc tctctcaaat gaacatggac gtccactaaa ccaggtgtga
    13141 tcaattgtcc ctttgcatca aacacttcat caaattcggc ttctgagaat cctgtaccga
    13201 ttgctttgat tcgcccatct tctatccaaa tttctgctgg tgtcgtcata ttttttcgtg
    13261 tattgatctg accgttttta atgagtgttt tcattttttc atcctcctat aattacgctt
    13321 ttccatttag cactgcttct aagattgcca ttctgacgaa tactccattg ctcatttgct
    13381 gaacgatccg cgactgtaat ccttccacta atgaatcagc taattctaca tcacggttaa
    13441 ctggtgctgg gtgcataatg attgcatgtt tttgcaactt cttcgcacgc tcaactgtta
    13501 acccatgttc ctgatggtag ccttcttttg agaaactttc ttttccatca tggcgttcgt
    13561 gttgcacccg aagcatcatc atgacatcaa cttgatctag tagttcatcc aatggcatat
    13621 aatgtccgta cacttcgaat tcatcgtcgt accattctct aggacctgag aagaatactt
    13681 gtgcgcctag acgttttaac atctgcatat tggatttagc tacgcgagag tgagtgatat
    13741 cacctacgat ggcaactttc agaccttcaa aatgaccaaa ttcttcataa atggtcatca
    13801 aatcaagcaa gcattgggtt ggatgctgtc cgcttccgtc tcctccattg ataatcgaac
    13861 actggattgt tttactttgt atcagttcgt cataatagtt ctcatcacca tgacggataa
    13921 ccgctacgtc tacgcctaat gctgacatcg tcagtactgt atcgtatagt gtttcccctt
    13981 tttggacaga gcttgtgctt gcttcaaatt caatcacttc tagccctagt tttttctccg
    14041 ctacatcgaa gcttttatgt gtgcgagtac tattttcgaa aaagaggttt gtagcaaagt
    14101 actggcgctt ttgcggagtc caattagcac cttgcttgaa ttcctgccct ctgcggatca
    14161 agcccataac ttcttgatca gtaagtgcct caacggtcaa taaatgcttt aagctgatac
    14221 gttctgattt gatgatcata atgtttcctc cttagttttc ttcgcttcgt gaagtttctg
    14281 gtaagataag atttaaaatg atgccaagta cagttgctaa tgccattgcc gacaatgtaa
    14341 atgtgcctac ttcaaagacg agtccgccaa ttcctactac taagataact gaagcaatca
    14401 acagattttt ctttttatca aaatcgattt tgttctcgat taaaatcttc attccacttg
    14461 cggcaattac tccaaataag ataaaactga ttcctgagat gactggtcca gggatactta
    14521 aaatcaaagc gcttaatttt cctacgaatc ctaaaatgac tgcaaatact gcagctccac
    14581 cgatcacaaa cacgctatgg acacgtgtga tagccagaac accaatgttt tcaccgtaac
    14641 ttgttacagg aggacctccg acaaaggctg ccacgatttg tgctaatcca tcacctgtca
    14701 acgtcttatg aagacccggt tcttcaaaga aattacgttt agttagttta ttcaatacca
    14761 taagatggcc gatatgctct gtcatcgtca caaaagcgat gggtgccatt gttgtgattg
    14821 ctccaagata tagctttggt tcatattgta caaagggaat ctcaaagtta ggaacagcaa
    14881 accaggcagc gtctacgatt ggcttagtat ccacgattcc aaacagcaag gctacgatgt
    14941 atccgctaat aatacctagt aaaatcggaa tcaatccgag aaaacctctt agccacatgt
    15001 tgaagaaaat tgttaatcct aatgtgatca gcgctacagc aatatacttg aaatcatacg
    15061 tgccctgatt atacatagca tttgttgctg catttgccgc aagtcccaac ccgataacca
    15121 tgatgactgg accgacgacg attggaggca agattttatc cagccatgct gaaccgatct
    15181 ttttgatgat cagcgcaaca atcaaataga ctaatccagt tgtgactgct ccttgtgcaa
    15241 tcgctggata accatctgtt ttcatcaaaa gctgcatcgc tgcaatgaaa gcgaagctgc
    15301 ttcctaagta cgcaggtatc tttcctttag tagtaaataa gtacaccaat gtacctagtc
    15361 ctgaactgac tagcgcaata cctggatcga tacctactaa gatggggact aacacggttg
    15421 ctccgaacat cgtgaacaag tgttgtaaac ttaatcctac ccaatgtcct ggggttgggc
    15481 gatcgtggat atctaacacc acgtcatcat tatgaaatcc ttttttctcc gacatgtttc
    15541 tctctcctct aatcttcctc tttcaaaatc atgatgcgat cttgtccatc aagctcttgc
    15601 atctctacta aaatctcttc cgcccgagat gttggaatgt tcttcccaac ataatctgca
    15661 cggattggca attctcgatg cccacgatcg accaggactg ctaatgaaat ttttcttggt
    15721 ctgccaaaat ccattactgc gtccattgct gctcgaatcg ttcgtcctgt atacaaaaca
    15781 tcatcaatca aaataacttc ttttccttct aaggaaactg ggatgtcaga agaatgaagt
    15841 tccggttctt ctggattttc ttttttgtca tctcggtata aagtgatatc cagttctcct
    15901 acaggaatat cgatatcttc tagctgcttc aggcgttcgg caattcttga tgcaatgtag
    15961 attcctcgtg ttttgatacc taccaatacg atatcttgga tactatggtt tctctcgata
    16021 atctcataag taattcgagt caatgctcgc ttcatggtta cctgatctac tacttctttt
    16081 gcttgcattt tcattccccc tacttttttg cataaaaaaa ctcctgccct aagaggacag
    16141 gaggaatgtt tagactgagc gtatactgat acactgatag ctatactatg tcaatgagca
    16201 atctacttat ggcgcaccat acgtatcctt gctcccaccc ttcttagcct cacgggacta
    16261 aacttaaagg aattctatta agttttatgc tttcgttgtt aattactata ccgttatcta
    16321 aattaaaatg caatgttttt tcttaatttt tctaaagttt tttcaaaaac ttctggaaga
    16381 ggtgcttcaa agatcatctt ttcgtttgta gtcgggtgcg taaaacctaa catttgcgca
    16441 tggagaaact gaccattgcc tttcaaagtt ttcttcggtc cgtatactgg atctccagct
    16501 aacggataac cgatatactt catatgcaca cggatctggt gtgttcttcc ggtttctagt
    16561 tttaattcaa ccagagtaaa tccttcgaaa cgttccaata cagtaaaatg tgtgacagcc
    16621 gacttcccat cttctcttat cgcctgcatt ttccggtttt ccttcgaacg gccgattggc
    16681 gcattgatgg ttcctttttc gtgaggaatc tctccatgga ctaaagcaac atatttgcgc
    16741 aaagaggtct tatcttttaa ttgctgagct aatgattcgt gtgcctggtc atttttggca
    16801 accattagca gtccggaagt atctttgtcg attcggtgaa caatacctgg acgaataacg
    16861 ccgttgatcg tagaaagatc cttgatatgg tacatcagcg cattgaccat tgtgccatgc
    16921 gtatgacctg ctgaaggatg aacgaccatt ccttgaggtt tgtttatcac cgctacatct
    16981 tcatcttcgt aaacgatcgt caacgggata ttttctggga ccacatctaa tgtttctggt
    17041 tcggggatca agacagtaat ctcgtctcct tgttttactt tgtaatttgc tttgactggc
    17101 tgtccattga cagaaaccgc tccatttttc agccacagct gaatttgcga acgactgtaa
    17161 tcggtgaata aattagtcaa tactttatcg atcctaccag attcatggtg aatcgtcgtt
    17221 ttaagttctg tcattttttt ctttgatcct tttcatctaa aataatataa attaacatac
    17281 aaatcactcc acatacaagt gtcacatctg ctacgttaaa aatcggaaag ctcataaaat
    17341 ctgtctggaa catatctact acatagccaa gacgaatacg gtcgataaaa ttgccaatcg
    17401 caccagctag aatcaaactc aatccaatca tgaaccagcg attacctttt tcacgatttt
    17461 taatcatcaa tgtgagaatc acaacgactg caatgagtgt cactatagta aagaaccaca
    17521 tcttcccttc tagcatactc catgcggctc cagtgtttcg aatatgattc agtgacaaaa
    17581 tacctggtat aagatttttc gtctctccaa gcgctagatt atcagtgatc caccatttga
    17641 ccaactgatc cactcctact agcaaagcgc tgaatatcca ataaataatt aacaaaaaat
    17701 caatcctttt ctatttgttt ataaatatca cgaggtcgta cgatcccgag cagattacca
    17761 tcgggattcc catctttagt gataagtacg gcttccaaag tcccttgctc acgaaacatc
    17821 gcttcaacct cgtaaatatg tgttttagct ggaacaaaat gataatttgt gtactttccg
    17881 ttttggatga tcaaatccaa cgctcgttta tttgttaaat caaccggacc tttttggctt
    17941 tcttttgcta gccaaaaccc gatattacgt aaagtaatca atgctacaaa ttttccttta
    18001 ttgtataacg gaaattgtga ataccttttt tctgctacta tctttaacag ttcaagtaac
    18061 ggaagatctt gttcaaaacc tgttacacgt tttgcaaatc gaggcagcac ggtttcaggc
    18121 tgaatcagtt cttgttcgat cagctggatt cgcttgactg tccaatcatt tggctctgca
    18181 atgacaaaat cgactgcgat ccgatcatgg acgatcgcat tccttaactg agcaagctgt
    18241 aaaagatcat cttcgtattt tctgatcatt aattgttttc tttgagctag gcgtctgaca
    18301 agttcggtaa atcccatatt tcgtgcattt cccatctcgt ctctcatcca tttttcgatt
    18361 cgattgaaac tgattaaaaa aagatcagag tttgccataa ttttcctcct tgatcaaata
    18421 tgcccggttt tttatataga aaaagaacgc aatcaacaca ataactgcaa aaaaactgaa
    18481 aaataaattt tgtaggaaaa cggcccccaa cccagttgct agaaataaaa acggatagat
    18541 ttgaagaatc aagtaattca ttgtgatcac ataagcaggc tttgcttctt tcactgtata
    18601 aacggggctt tttagccaat aaaaggtgac ctgcatcgta aagggcactt ccagatcaag
    18661 cgttatcttt tgttcatgac tgagaacact gcgtttttca ttgttgattc ttagctgaat
    18721 cgagctaccc ataccgtaaa aaccggtttt tcttataaat gtcgttttca tcataccctc
    18781 ctattatgct ttagtatagc tcaaaataca ataaagaaaa agaatgatcc tagtgtaaac
    18841 aaaattaatt ccctatcgtc taaaaaaaga agctgtagtc aaaatacagc tccttcgatg
    18901 gtagattata atcaagctta aaacaatccc aacacttctc cgtcatttgt cacgtccata
    18961 ttcaatgcag caggtttttt aggcaaacca ggcatcgtca taacatctcc agtcaaggct
    19021 actaaaaatc ctgcccctaa ttttgggatg atttcgcgga tcgtgatggt gaaatcttcc
    19081 ggtgcaccta gcagtgaagg atcatcagaa aatgaatact gtgtttttgc catacaaatc
    19141 ggcagtttgt cccatccatt cttcgtaaac tcttctattt gtttttgcgc ttttttgctg
    19201 aaaacaacac catttcctcc gtaaattttc ttgacgattg tttctgtctt ttcttgtatc
    19261 gtttcttcta ggcggtataa tggcttgtaa ttagcttctt ttctatcaat caatcgtact
    19321 acagcttttg ctaaatcaac gccgccttct gctccatctg cccatacact cgctctcttg
    19381 gcaagaatcc cctgattttc acacaagtgc tccaacagtg tgagttcatt gtctgtatct
    19441 gttacgaatt cattgattgc gacgatcaca ggtagctgat attgttccat attacgtata
    19501 tggcgcttta aatttgcaaa acctactttc aaagcatcaa gattctcatt tttaagttca
    19561 tcttttttca ttccgccatg cattttcaat gcacggacag tggcgacaat caccacagca
    19621 tccggcgctt ttttcaagtt aggtactttg atatctaaaa atttctctcc gccaagatct
    19681 gcaccaaatc ctgcctctgt gacaacgtaa tctgctaatc tcaatgctgt cttggtagct
    19741 aaaatactat tgcagccatg agcgatattg gcaaacggtc ccccgtggac aaaagctggt
    19801 gttccgtaaa ttgtttgtac aagattcggc ttgattgcat ctttcagcag taatgccaga
    19861 gcgccttctg ctttcaagtc tccagcagtc acaggctgac ggtcaaatgt atagccgaca
    19921 acgatccttg caagacgagc ctttaaatct tcaaggtctg ccgcaagaca caagatagcc
    19981 atgatttcgc tggcaactgt gatatcaaac ccatcttctc ttggaacacc ttggattggc
    20041 ccacccaagc caacaattac tttacgcagt tcacgatcat tgagatcgac tacccgtttc
    20101 caaatcactc ggcgggcatc gatgttcaat tcatttcctt gatgaatatg attatctaaa
    20161 agagcagata atgcattatt agctgttgtg atagcatgca tgtcaccagt aaaatgcaag
    20221 ttgatatctt ccataggcaa tacctgtgca tatcctccgc ctgcagcacc acctttgatt
    20281 cccatcacag gaccaagtga gggttctcgc aatgcaataa cagatttttt attgatttta
    20341 ttgagcgcgt ctcctaaccc tacagtgatc gttgactttc cttcgccagc tggtgttgga
    20401 ttgatggaag taactaagat caaatgcccc tcagcgttat cctttgatcg attgattcca
    20461 gaaaaattga ttttcgcctt atatttccca tacagttcca aatcgtcttc cttaagatct
    20521 aatttactag ccacttcaac aatcggttct agctctgttt cttgtgaaat ttgcagatca
    20581 ttcttcattt gattacccct tttcacgaac tttattcaac aaaatatctt ctttgttaag
    20641 atatgccaat tatacctttt tttattcaga aaaaaaacat aaaattacag gtttttaatt
    20701 tatttgacgt acttataaat actctttata atgaaggcta tgatcagact ttagaaaggt
    20761 cgtgagtgct ttgaccacag gaattgtaaa atggtttgac aacaaaaaag gatacggctt
    20821 tatcagttat gatgacacag aagaaatttt tgtacatttt actgcgattg aagaagaggg
    20881 attcaaaact ttggaagaaa atcaagtggt cgaatttgag atcatcgaag ggaatcgagg
    20941 aacacaagct gcccatgtca agaaaggcaa cgttcagact accgatacta gtatgcatga
    21001 atagataaac aaatagagtg agacgctaaa cgggatgaac cgtacgtctc actcttttct
    21061 tttcctgtaa aaattcgttt ttttaggttc tgatttttaa aattagcttt ttcttttttt
    21121 cgtataagga taaggaattg attctgattt gggtccgttt ttttcaaaat gatcaaaaag
    21181 gactacttga tctttttttc gcatcatgaa aacagatggg gctttctcat cttgcaacat
    21241 catttgcacc ccagtattag ttggcgtcaa agatcgaatc tgtccgacag atatttctcg
    21301 gtctttccaa aaagcagcat aacgtataat cacagagttt gattttaaaa taaaagaacg
    21361 ccgcaaacct agaaatagaa aaacaagaaa aatacctaaa acaaaattgc ttttccaata
    21421 aggacctgtg ttttctaaag aaagaatcag actaaaaaac agaatgatca aggtcaatga
    21481 ccaatagatg atcgtatgtg ccagttcagg ctgccatcga atattttttt tcattctcta
    21541 acctctcacc gattcagtaa tgatataatc ttaccataac attagtttga acaaaaagga
    21601 gaatctcttt ttatgttaaa agtatacatt gatgcctcta caaaaggcaa ccctggtcct
    21661 agtggaggtg gaatcttgat tgtctatcaa ggtaaacaag aacagctatc tatcccgctt
    21721 tctataggga caaaccatca agcagagttc gaagtctttt taaaaacttt agaatttcta
    21781 aagaaaaacg acttacagaa tgaaacgatt ttctgttttt cagacagcaa aacactagtt
    21841 tctacggtcg acaaaaacgc aacgaagaac gagctcttcc ttccctacct tcaaagaatc
    21901 caagaacttc tatccgaatt ttctttattg atcctgcaat ggttgcccga aaacaaaaat
    21961 aaaggagcag ataatcttgc acgtcaggct ctccataaac aattaaaaga aaaaaagact
    22021 ctttaaatta aaaactttaa ggagtctttt tatagtttct catttaatat aaaagataat
    22081 tgtgatcaaa cgtaatttcc aattggtaat ctccgatttt ttcttctatt tgactacgta
    22141 agattctttc tagttctgta tcaacaaact ggaatttctc agggaccact acatcaaatt
    22201 gcagcgtttt tccttgatga attactctaa aatcatgaat attaagccct gtacctgttt
    22261 caagaagaat ttgctttaac tgccgcaaga tattatagta tttttcgtta tcaacaggca
    22321 ttggatccaa gtgacagaca agttccacat ccaaagactt tttaaagtct ttttctatca
    22381 gatcaataat cttatgagcc acattcaaat tcattgattc atctacttcg atgtggactg
    22441 atgcaaagcg tttattgggt ccatagttat gcaccaataa atcatgatag ccaagtatat
    22501 catcgcaagc atttaaatgc tcttccattt cttcaatctc tgctggcgtt gggcgacttc
    22561 ctaatagttc gtatacaaaa tttcgaagca tcgtcaatcc actataaagg atatataatg
    22621 ctaaagcaaa acctatatat ccatcaatac gccatccagt tacccattcg attgctgctg
    22681 aaagcaatac agccagtgta gtaaagacat cattataact atctgttgct gtagctctca
    22741 atgtttcaga atcaatggat tgcgcaataa cctgatacat tttgccttgt gcaaatttca
    22801 aaaagatgga aatgattagg acaaaaaaga cgatgggtgt taaagagaca ttttctggat
    22861 ggatgatttt ttcagcagaa gactctaaaa attgaaatcc tacataagta atgatgatcg
    22921 aaacaaataa cccactgata tattcgaagc gttcgtgtcc ataaggatgt tcctgatctg
    22981 ctggctttgc agcaatttta aatccgatca aagtcaatat ggatgaagca gtatctgata
    23041 gactgttcac tgcatcagcc ataatggaga cacttcctga aaaaagcccg atcattagct
    23101 tagagacaaa caagatcaga ttcgtaacca atcctaagat acccgctagt actccttttt
    23161 ttgtccgctg ttgcggtatt tgtttctttt tttcttcttg tttcttagcc aagtttttta
    23221 ccccttctag tttgcttgtt gctcaggtaa aaagatggag aggacgattg ttttcctctc
    23281 ctctcttcct ttttctatct atcgtgcgtc ctattactat taaaagatgg tcattattct
    23341 ggtttcaacg gtgttgctgc aaataacgct tttgctagta atggtgttat gattcccgca
    23401 cctattgctt ctggtatccc attgattgct gcaacccctg ctaaggtttt aaataaaaga
    23461 gcagagtcta caccataggc atctgccgtt gcacctgtat aaaataatcc catgaatcct
    23521 aacactaaga ctgtattgac caatgaaccc agtacccctg caaaggctga gctgatcgtt
    23581 attgatttat ttttttgata aatccagtgg aaaaccaagc ctgctactaa gccaacaagt
    23641 actctgggaa ctaccgagat aatcggattc gtgaacacta atgtatcaaa tggcgttgtt
    23701 ggcatggcat aggcacgaac aattgtagcg attccccaaa acaagccaat caccatgcca
    23761 tcttttgggc ctaatactac tgcagcaata attactgtga tatggataat tgttaaacta
    23821 atgaatccta atggaataaa ccccagccaa ggaaccatcg cttgtacgat gatgattgct
    23881 aacaagatcg cacgtaagac tagtcgaaag gtcttatttc tactattcat tttttcaccc
    23941 ctttttattt atactaatcg taaaaatttc tgtcactttc gtcaactaaa gaccatccga
    24001 atccgaccat ttgattagat gcgccatcca attgaaaaaa gtatgagggc aagacttgtt
    24061 tgtcttcgcc cccataatca tgtttcgttc aatgtttgaa cttttgtttt tctgtcaaac
    24121 gtaaacgccc tattgttgcg caacccattc tttcgcagct ttaagtgcat ctggaatacc
    24181 agcaggattt ttcccgccag cttgtgccat gtctggacgt ccaccaccgc caccgcctac
    24241 tttcggtgcg attgctttaa tcaagtcgcc agcttttagt ccagcttcat ttgattcttt
    24301 cgtcattgcc gctagcagac tgacttttcc ttcttgttcg tttgctaaaa caagaatatc
    24361 tgaaatattt ttttgtttcc attggtcagc taattgacgc agctgattca tatctttcac
    24421 ttttacttgt gctgcaatga ttgtttttcc attgatcgtt tctggatctt tgaatacatc
    24481 accagcttgt tgattcgcta acttgctagc taattgttca ttttcctttt gtagctctct
    24541 tacttgctgt tgcaattgtt ccgcttttga aacaacttct ttcaattgag gtgatttaac
    24601 aattgttgct acttgtttta gttgtttttc ttcttcttca agtagttcgt acgcttcttt
    24661 acttgttaca gcctcgattc gtcgcacgcc tgcaccaatt cctgattcag aaacgatttt
    24721 gaagattccg atgttttctg tatttgctac atgtgtgcct ccgcacagtt cgattgacca
    24781 gccgccaata tttactactc gaacgtcatg tccgtatttt tcaccaaaca aagccattgc
    24841 acccatgttt ttggctgtat cgatatctgt ttcaattgtt tcaactggga tagcttccca
    24901 gattttttcg ttgacgattc tttccatttc agctaattca tctgaagtga tttgaccaaa
    24961 gtgggtaaag tcgaatcgta aatgtccagg agcgactaaa gaaccagctt gatttgcatg
    25021 ctcgccaagt acttctttca atgccttgtg cagtagatgg gttgctgtat ggtttttgat
    25081 gatccgattg cgcatcgatt gatcgatcaa tagatgatag gtttgtcctt cactcattgg
    25141 tgcttttact tctacggtgt gcataaattg accattaggc gctttttgaa catctgtaac
    25201 aactgctacg atttcgccta cttcattcat gatccatcct tgatccgcta cttgcccacc
    25261 catttctgca tagaaaggtg tttgatcaaa gatcatttga gcttcacctt ctgcgacttc
    25321 attcaaaaga gactcacctt taacgatgac caataattga ctatctgatt cttgttctgt
    25381 gtatccaatg aatttacttt ctactttgat atctgtcaat aaagcagatt gtacccccat
    25441 tgatttttca gtactgcggg cagagcgagc acgttcgcgt tgtgcatcca tttctttttc
    25501 aaaaccagca tgatcaactt tcagtccttc atcttctgcc acctcttccg ttaattctac
    25561 cgggaagcca tatgtgtcgt atagtttgaa gatgtctttt cctgctagag tcgtcccgtt
    25621 ttcgtttttt acttcttgga tcacttgatt taagatttcc aaaccttcat tgattgtttc
    25681 attgaaacgt tcttcttctg tacggataac tttttcaata aattcttttt gttgaagtac
    25741 ttcaggataa taacttacca tgatttcccc aacaacaggg actaaacgat ataagaatgc
    25801 ttgttcaata cctaattttt tcccatgcat gacagcacga cgaagcaaac ggcgtaagac
    25861 ataccctctc ccttcattcg atggcaatgc accgtcgcca atagcaaatg aaagggcgcg
    25921 aatatgatct gcgattactt tgtaagaaat atctgttact gggttttcac catattttac
    25981 ttgtttgctt agcttttcta cggcgtggat aataggcata aacaagtctg tctcaaagtt
    26041 cgttgacgca tcttgaacga ttgagaccat gcgttccaat cccatccccg tatcaatgtt
    26101 tttatgagga agtggttcaa atgtgttatc aggcttatgg ttgaattctg aaaatactaa
    26161 gttccagatt tctaaataac gttcattttc tcctcctgga tagttttcag gatcgtcttc
    26221 tgctaaatcg ttataagctt ctccacggtc atagaaaatt tccgtatccg gaccacatgg
    26281 gcctgcaccg atatcccaga agttgtcttc tacatcaata atgtgatcta gtggcaggcc
    26341 aacttcttca tgccagatgc gttttgcttc tgtatctttt ggataaactg ttacataaag
    26401 tttttctgga tcgaatgcca tccattttgg atcagtcaaa aattcccatg cccagtggat
    26461 ggcttcattt ttgaaatagt caccaatcga aaaattaccc agcatctcaa acatcgtatg
    26521 gtgccgtgct gttttaccga cattttcgat atcatttgtt cgaatagatt tttgggcatt
    26581 tgtgattctt ggattttctg gcacaactga accatcaaaa tattttttca atgttgcaac
    26641 accagagttg atccacaata aggttggatc gttcacgggg atcaaagacg cactaggttc
    26701 aatcgtatgt cctttagatt ggaagaaatc aagaaacatt tgtcgaactt gtgcactact
    26761 taattgtttc atgtcattac ctgctttcta taaattaaat acaaaaaaac accatcacag
    26821 ccaaggacga aaactcgcgg taccaccttg attgcaatga tgtgtaatca ttacctctta
    26881 aactcgataa cgctgagctg cgtcggtatt tctaccaaaa tagaaaaagg gagcaatttg
    26941 attagtgact attctttttt cagccaagaa agaattttct gtgagtctta aaaaacaaat
    27001 catatccttt tcgtacttaa ttagtatacg cattctattg atcactgtca atatctcaat
    27061 ttttattttc atttcctttt ggtcatactc cacgattttt ggtacgataa gaataaagga
    27121 atcaatacat ctctgctatt catgacaagg aggaatatga tgaaaaaaat agttggagcg
    27181 gatgaatgga tacgcgcagc tagctgcttt ttgagatatc aagtttttgt attagaacaa
    27241 gggattcctg cagaactaga attcgacgga aaagatatcc cgcaacaaac ctattatcta
    27301 tttcttgatc aaaaaagacc agtagctact ttgcgttatg aaaagaaaac acctacttgc
    27361 ttcaagccgg aacgattttg cgtagcaaaa gaataccgag gaaaagggtt aggcggtcag
    27421 ttgcttgaag ctgctgaaaa acgggcggtt tcagaaggtt gtcagactag ttatgtagta
    27481 gctgaaatga cggctgtaga tttttatgaa aaacaagggt atcatacttg cacagaacct
    27541 tttattgaag atgaagtact ttgtatcgga atgaaaaaaa gtctagctta aaaacaagag
    27601 attgtgacac gccttttgtc acaacctctt gttttttatt tatgtgaatt tttcttattt
    27661 ttacgagcag tacggctttg ctgtctgcgt tcgattttgc gttctttttt atttttttct
    27721 gcaattgccc aatcgatttt tttcttataa ccaggcttga tctttttctt tttcttcttc
    27781 acaagaccaa tcagcgtggg atctaataca tcttttgatt tttctcgttt ggcacgtcta
    27841 ttacgatcgt atgttttgac gatttccccg tttttgattt cttttggttc aaatgagacg
    27901 cccaattttt ctatcgcatc aatggcttgc tcatcatttg gcgaatataa cgtaatggcc
    27961 gttcctttta agccattacg tccagtacgt ccgacacgat ggataaagaa gtctagatct
    28021 tctggaattt ctgcattaat aacatgagag acaccttcga tatcgatccc tctagcagct
    28081 aaatcagtcg ctacaacata ttgatactcc agattctgta cttgtcgcat cactcgtttt
    28141 ctctctcttg gggtgatgtc tccatgaatc tttgctactt tcaagccttg gtttttcaag
    28201 taatcggcaa tttcatccac atgctgtttt gtattggcaa atacgattgc taaataagga
    28261 tgaccaattg tcagcagttg ataaatcatt tgattctcat ctttaccttt tgttgaaatc
    28321 agccaatttt caatgtcttc agaaataact gcttttggtt tgatttcttc aatgattgga
    28381 ttttccatgt attttcgtaa gaatggacga agtttttctg gaatagtggc agaaaaaact
    28441 aaaaactgca gcttttctgg taatcgactt gcaatttggt ctacttcatt taaaaaaccc
    28501 atatccaatg tcatatccgc ttcatcgaca acaaatgcaa atgcggtgtg aatcttcagc
    28561 gcttgttcat tcatcatgtc taatattcta ccaggcgtgc ctactacaac ctgtggctgt
    28621 tggtgatgca gacgatttaa ttgacgctgt ttgtctgtac cgccgacaaa attcgagaca
    28681 cggatctccg gtgtagaaaa tttagcgatt tgtgtagctg cttcatagat ctgtgcagct
    28741 agttccctac ttggagctgt aataacaact tgtacttcct ctttttctgc attgattttt
    28801 tcaaacaaag gcaacaaaaa cgtatgtgtt tttcctgtac cagtttgaga ttgcccaatg
    28861 atattttttc cttttgcaat taaaggaatc aaacgttcct ggacctctgt gggctgtttg
    28921 aagccctttg cggttaacgc ttcattgata aacggtttga agcggaatat atcaaaatta
    28981 ttcattcgtg cacctcttct ctttaaatta tcacgacttt ggcattataa cacagacaaa
    29041 gcgtttttgc tcagctaatt tcgagaatca tcatcctttg tttggccaat cgtgctaaca
    29101 gtatatgtgt gactagcttt ttcataaaac agaatccgtt tttttctcaa aaatgatagc
    29161 aagatattta aaataaaaat accttgtagt aacaagacac cgagagaaat gaatactgca
    29221 ctcatctgca acaccgtatt cgttgtcgaa ttcatgattt gctgaagcaa ctgccccaac
    29281 cagcttaaac cataaaaaag aaagtacaga tacccgtagc ggatcatcaa tgcttttaaa
    29341 gaaattcgtt cgcggtcttc ttccaccaaa cggatacgca caattttttt gccaagtgtc
    29401 catccattgg ttataaacgg tacaatcata aagtataccc ctacttctaa taaaagccac
    29461 cagatatgag aagtaactgc ccctgaatcg aacgcaattt taaagaaaat cgccgctacc
    29521 aaattaataa gagataaaaa tgtccagtcg atcaaataag cgaccaagcg tcgagtcaat
    29581 gagacagtct ttcctttttc ataagaaagt tcatccattc gtcttcttgt aggaagcata
    29641 aatgtaaata acggcgtaac gacaaaacca atgattccac ccaatgtatt tgtcactaaa
    29701 tcattcacat caaataagcg gtatggtctt gggtagataa aataaagtcc acttagctgc
    29761 gtcaattcaa aaaatagaga aaggcagaaa ctagcaatca tcgttttcat aaaagaaagc
    29821 cggaaataat atcttaaata aatgccaaac ggtatgagta ataaaacatt aaaagctggt
    29881 tccaaaaata cactctgctt cattgccggc aggtaggtac taggttcaga caagaccaat
    29941 accgtgttgt ctctgaaatg ttgccatgaa gcaaataaat gcagctccat ctttggccct
    30001 gtcatttggg caaccgtctc ctttgccgga agtggtaaga tcaccaaaaa atacgcacac
    30061 aataaataaa aaacgaaaga atacaaaata actgcccttg ttactagaaa tgttccatac
    30121 tttcgatatt cataaataaa aaaaacagat gagatcgcta aggctaaaaa tggaaaaaca
    30181 atcagcgcaa aacgaatcgg ttcgatataa cttgccatat gactcctcct tgattctttg
    30241 tatctctctt atcttagcag tcctttgctg tttcaagcaa gtaacgaggc tattatttct
    30301 catttctttc aaagtaaagt aagataaaca caaagggggt gtcgagatgc tgaatatgta
    30361 ctttgttttt ggcgtaccta tttttttact gttcctttat gctacgatcg cttatgtaag
    30421 aaaaagaact acaattcatt atttaggatt tattctcctg attatttcgg gtttcatgct
    30481 agtcttcaat cttcagactt ggcagcaagc acttcttgaa atggataaaa tgactccaca
    30541 tgctttgtct aaagtgcttg gctaccctgt ctatctgatt tggcttccca ttttcatttc
    30601 tggttgcttg gttttattaa atatttatcg cggcgtacgc cgaatagttc agttacgcaa
    30661 atcaaagtaa aagacagaaa tcggttgagc ccgatttctg tcttttactt tgctatttca
    30721 tcgtagacaa aacgacattt acgtgatcaa aattaacgag tacttttgac atttcttcca
    30781 cgtactcctc atttaaataa aatattttgt ttggcagaga agtgcagttc taataaaatt
    30841 cgaatgtttt agctatctac gttttaaata gagataaatc gggataccag caagtgtcaa
    30901 tactatcccc atcgaagcta aaaaggtctg agtgataagc gtgcttgata cgataaaagt
    30961 ccctccaatg attgctacta gtggaataaa cggatacatt ggaactttgt atggtcgaaa
    31021 caaatcaggt tcttttttac gaagaagaat gacaccaaca aagaccatcg tataaaaaat
    31081 ccaaatgaca aagatcagca tatccgttag tgtatcaaaa ccaccgatca tcatcatgcc
    31141 aatagcgatg actagctcta agatacctgc ggctacagga actttagttt tatcatgaag
    31201 tttagcaaag agcttgctaa aaggcaggtt gttttctttt gccataacat aagggagacg
    31261 cattccagta agggtatagc cattgatggt tccataaaca gaaatcaaga taccaactgt
    31321 gaccagtctt ccaccgaagc cgccaaaaat catttttgca acgtcacttg ccgcattgct
    31381 gtttcctgcg actccgtcga tagatgcggt tcttaagaac accgcattta ctaataaata
    31441 aacgatcatg attccgatga ttcctaaaga aattgctttt ggcaaatctt tcgccggctt
    31501 tttcaattct cccgaaatat ttcctacgtg tatccaaccg tcgtatgcga acatcgttgc
    31561 tagcaagccc gctcccaagg ctgagaaaaa tgaaagattt tctcctgctt ggatcggaaa
    31621 cagttggaag tctactcctt cttgtcggaa caaaccgaaa atgacgatca caaacaaagg
    31681 aataagcttg cagacaagcg taatcgactg aaaagcccct ccagctttag aacctaaaaa
    31741 attgatcaac aggatcgata cagcagctgt aacagctacg ggaacaatca ttgactggga
    31801 aagaccaaat agattgacga actgtgtacc gaaaatgatc gacaaggctg ctacatttgc
    31861 tggaaaataa atcacgactt gcgcccagcc caataaaaaa gctgctgtat ttccgtaaat
    31921 ccgttcgata tatttaatca tccctcctgt ttctggaata gcagctgcca gttcagctcc
    31981 tgttagtcct gcacagacag aaatcatacc accgagaaac caagaaaaca tatgcaaact
    32041 ggcggatcct gtaacttctg ctacgcttgc ggctttaaaa aatacccctg caccgatcac
    32101 tgtgcccata acagtcgata aagcagtaaa aaatcccatc gtacgtttta attcattttc
    32161 tatttttact tcttttactg gcgtggcttg atattcttcc atcccttgtc ccctttcata
    32221 ccatagccaa tcccaaaagt ataaaaaact ttttggatgt ggttcttgtt ttataccgtc
    32281 tctaccaatt tttctttcag tttttccaac atatcctttg tcatcacatc taagtcgtat
    32341 ttcggcgaaa agccccattc ctcttgcgca caagaaacat ctagtgaatt tggccaagat
    32401 tctgctattg actgacgtac aggatccaca tcataatcca tttcaaaatc tggtatgatt
    32461 ttttgaatag atgctttgat ctcttccggg tcaaaagaca tcgccgatat attgaaggca
    32521 tttcgatgaa ccaatcgatc aggatctgca ttcatgagtt ttataatggc atcgatcgca
    32581 tctggcatat acatcatatc cattgatgta ccttgatcaa tgaaactagt gtactttcct
    32641 tctttgattg ctgaataata aatatcgact gcgtagtctg tcgtaccgcc tcctggcaag
    32701 gttgcataag aaatcaatcc agggaaacgg acaccacgag tatctacgcc atatttcaca
    32761 taataataat cacatagtag ttctccagct accttcgtta ccccatacat ggttgtagga
    32821 cgctgaagag tatcttgtgg ggtatgatct tttggagtat ttggaccaaa cgcgccgatt
    32881 gaacttggcg taaaaaattt cagctggaat tctctagcga cttccaatgc attgaccagc
    32941 ccagtcacgt tcaaattcca ggctaattgt ggttttgctt ctgcaacagc tgacaaaaga
    33001 gcagccaaat gaatcaatgt gtccacttga tattgttctg ctatgctgcg cattttttcg
    33061 taatccatta catctaggat ctcaaagata tcgtttttct caatgacttc aggcatgcgt
    33121 atatctgtcg caatgacatt ttcactgcct agttcttgac gtagccgtgt gatcaactct
    33181 gaaccaattt gtcctaaaca tcctgtcacc aatacttttt tcatgatgat cctccctgat
    33241 cttttttact actatcttca gatgattaac aatatatgcg tgaacatgtt ctctaatttt
    33301 aaatagacga aaaaactaaa tttagttaaa taatttccat ttcttttcct attttttgat
    33361 aaactgctac tgcttcatca agcatttccc tcgtatgttc cgcagtcggc atatttctga
    33421 tcctgcctgt cccaagaggc acagttggat aaacgattgg tttagcataa atcccatgtt
    33481 cgatcagctt cttagagaaa gtctgagtca ctttttcgtc tcccaaaatg actggagtaa
    33541 taggtgtttc agacataccg atgttatagc ctactttttt caattcttct ttaaaataat
    33601 tagcattttc ccaaactttt tcgacaagtt ccggatgttc ttgcatcaaa gtaatcgaag
    33661 ccaatgcagc tgcagctgca cctggagtta gcgaagtaga aaataggaat ggccgacttc
    33721 tagctttcaa ccagtcgatt agtgttttcg atccagctac ataaccccca acaacaccta
    33781 tagctttcga taacgtgccc atttgcatat cgattttgtc gcttaatcca aaatgcttga
    33841 cggttcctgc accttttcca gttacgcctg agccgtgcgc atcatcgacg taggtgatca
    33901 gtccatattt ttcaacaatc ggaataattt ccggcaatcg tgcaatgtct ccatccatgg
    33961 agaaaacacc atctgtgata tacatgattt ttttatattt cttactttct atcgcttcct
    34021 ttgctttttt ctcaaggtct ttcatatctt gatgtttaat tcggatgatt tttgctccgg
    34081 ataagcggca accgtcaata atagatgcat ggtttaattc atccgataag atagcatctt
    34141 cttttgtcat tactgcagag atagcgccca tattgcagtt gaatccagat tgaaaggcga
    34201 ttgctgcttc tgtccctttg aattctgcga ttttttcttc taattgctga tggattttca
    34261 gcgaaccatt gatcgttcgg actgctcctg ctcctacacc ataagtttcg gttgcttcaa
    34321 tacatgcttt tttcaattct tcgcgattgg caaatccaag ataattgttg gatgctagat
    34381 tgatcatctt ttttccgtct acgatgatac aagcaccatt ttcactttct agtatgtcga
    34441 tcgtattgta taaccccttt tctttcagtt gatccaattc tttctctaaa tacattgtta
    34501 gttctttgga agacattgtc tccacctcca tattcatctg tcttgcagaa cataccaaac
    34561 atatatcgtg tcattcatct acaaagcaag tatgctatat ttttgataac gcttacactg
    34621 attatgagaa ttatttaacc acatttttta tttacagtat ctttttactt tcaaatgact
    34681 tatatagtat gaacaattac cttttagata atgatctatt ttactctacc tgggcagata
    34741 aatacaacac aaccattgat agaagccctt tcgttaaaat caattgtatt ctgaatattt
    34801 aatttattta ttgcatagac caataaaaat acataaaatt tccatcttcg tttatcagca
    34861 atttttaatt ttactctctg aatatttctt aaaagtttta cttgttgaat tcgaaaaaag
    34921 gactgtgaca aaagtcttgt cacagtccta ttcttccga
//
LOCUS       DANKKG010000017        31376 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000017 DANKKG010000000
VERSION     DANKKG010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.017"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            188..634
                     /gene="nrdI"
                     /locus_tag="PW004_000683"
     CDS             188..634
                     /gene="nrdI"
                     /locus_tag="PW004_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HDL2725676.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(699..2681)
                     /locus_tag="PW004_000684"
     CDS             complement(699..2681)
                     /locus_tag="PW004_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2725677.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(2695..3591)
                     /gene="murQ"
                     /locus_tag="PW004_000685"
     CDS             complement(2695..3591)
                     /gene="murQ"
                     /locus_tag="PW004_000685"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDL2725678.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(3594..4655)
                     /locus_tag="PW004_000686"
     CDS             complement(3594..4655)
                     /locus_tag="PW004_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDL2725679.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(4866..5432)
                     /locus_tag="PW004_000687"
     CDS             complement(4866..5432)
                     /locus_tag="PW004_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDL2725680.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            complement(5460..6962)
                     /gene="eat(A)"
                     /locus_tag="PW004_000688"
     CDS             complement(5460..6962)
                     /gene="eat(A)"
                     /locus_tag="PW004_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HDL2725681.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(7220..8341)
                     /locus_tag="PW004_000689"
     CDS             complement(7220..8341)
                     /locus_tag="PW004_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDL2725682.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            8467..9408
                     /locus_tag="PW004_000690"
     CDS             8467..9408
                     /locus_tag="PW004_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDL2725683.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(9558..10454)
                     /locus_tag="PW004_000691"
     CDS             complement(9558..10454)
                     /locus_tag="PW004_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HDL2725684.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            10723..12063
                     /locus_tag="PW004_000692"
     CDS             10723..12063
                     /locus_tag="PW004_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2725685.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            12281..12724
                     /locus_tag="PW004_000693"
     CDS             12281..12724
                     /locus_tag="PW004_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HDL2725686.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(13228..14646)
                     /gene="nhaC"
                     /locus_tag="PW004_000694"
     CDS             complement(13228..14646)
                     /gene="nhaC"
                     /locus_tag="PW004_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDL2725687.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(15049..16272)
                     /locus_tag="PW004_000695"
     CDS             complement(15049..16272)
                     /locus_tag="PW004_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDL2725688.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(16303..17142)
                     /locus_tag="PW004_000696"
     CDS             complement(16303..17142)
                     /locus_tag="PW004_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDL2725689.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(17159..17839)
                     /locus_tag="PW004_000697"
     CDS             complement(17159..17839)
                     /locus_tag="PW004_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2725690.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(17841..18914)
                     /locus_tag="PW004_000698"
     CDS             complement(17841..18914)
                     /locus_tag="PW004_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2725691.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(18970..19208)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(19251..19484)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(19561..20232)
                     /locus_tag="PW004_000699"
     CDS             complement(19561..20232)
                     /locus_tag="PW004_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL2725692.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(20319..22997)
                     /gene="mgtA"
                     /locus_tag="PW004_000700"
     CDS             complement(20319..22997)
                     /gene="mgtA"
                     /locus_tag="PW004_000700"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HDL2725693.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            23656..24093
                     /gene="ndk"
                     /locus_tag="PW004_000701"
     CDS             23656..24093
                     /gene="ndk"
                     /locus_tag="PW004_000701"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HDL2725694.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(24115..24981)
                     /locus_tag="PW004_000702"
     CDS             complement(24115..24981)
                     /locus_tag="PW004_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL2725695.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(25141..27234)
                     /locus_tag="PW004_000703"
     CDS             complement(25141..27234)
                     /locus_tag="PW004_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDL2725696.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(27983..28606)
                     /locus_tag="PW004_000704"
     CDS             complement(27983..28606)
                     /locus_tag="PW004_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725697.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(28765..30162)
                     /locus_tag="PW004_000705"
     CDS             complement(28765..30162)
                     /locus_tag="PW004_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL2725698.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(30194..31165)
                     /locus_tag="PW004_000706"
     CDS             complement(30194..31165)
                     /locus_tag="PW004_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2725699.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
BASE COUNT         8913 a         6618 c         5463 g        10382 t
ORIGIN      
        1 cgcttttatc aaaaagtata acaaaccttt tttattttta cttaattaat aaaaacttta
       61 atagaaatat aattctaccg gcttttttct cattatacaa gcaaagcgcc tagtcccttt
      121 ccaaattaca ccaccaaaaa acttaaaaaa gatgctatac taaaaggaaa gaattggagg
      181 atgatccatg aacattttat atatatcgat ttcaggaaat acgcgtgcct ttgccaaaca
      241 tctagcagaa tatgctgaaa aaatgcatgc agaagatcct gtcaatcctg aagttacttt
      301 aaaagaaatc catgaaaata gtgattttac gaaagaaaca gaaccatttt ttactttcgt
      361 tcctacttat ttagacggcg gaaatggttt agataacggt gatacagaaa tcttgaccga
      421 aacgatgcgt gaatatctgg aacatgaaga caatcataaa ctttgtcttg gtgtagtcgg
      481 tagcggaaat aaaaacttca ataatcagta ttgtctaaca gccaaacaat atgcacaaac
      541 atttggcttt cctttcttgg cagattatga attacgggga acaccatctg atgtgacacg
      601 aatttatcag attttagcag aaaacaataa ataataaaaa agaagaagct gaaaaataga
      661 tttcagtttc ttctttcgtt tctattgttt ctatcatatt aagctttgac atccatcaca
      721 ggtgcttttg cttttgcttt acctgttttt tccaagacaa agttttttac acgatccata
      781 ttggttacca agacgatcat ggatgtttct ttgcctgctg ctttgatcgc ttctaagtca
      841 gcttctgcta caagcgtatc aggtgtcact tgctgccctt ctgttacttt cacatcaaat
      901 ggcgtaccgc ctaagtcaac tgtgttgatt cccatatgaa gcaagatttc taaaccattt
      961 ggcgttgtga tcccaatagc gtgtttcgtt gggaagacag agctgactac cccagtaact
     1021 ggagaataga tatgtccgtt ttctggttct acaaagtagc cttcacccat catcttttgt
     1081 gcaaaaactg gatcacttgc tttatctaaa gaaacaactg ttccgtcagc aactgatgag
     1141 aggatagtca cactttcagc tgaagtttga ggtactactt tttcaggcac ttcatttact
     1201 gtggctgttt cagtcgctaa ttcttctttt tctaaacggt ctttaggaat accgaagaag
     1261 aatgtagcaa cgaagccacc agcataagct gccaataatc caaggatgta gcctaaccat
     1321 ttaccgtcag agatcaatgg gatcaaagcg gctcctgaag gtccaatagc gatagccccg
     1381 acatttccga tcattccgat cactgccccg ccgatacctc caccgataca agcagtaata
     1441 aatgggcgtc ctagaggtaa tgttactccg taaatcaatg gttcgccgat tcctaggatc
     1501 ccgactggta aagctccttt gatcatttct gttaattctt tatctttttt gcagcgaatc
     1561 cataatgcca atgctgcacc cacttgtcct gcaccagcca tcgcaagaat tggaagcaat
     1621 agcgtacgac cagtttcatc aatcatttgc acatggattg gagtcaaaat ttgatgcagt
     1681 ccgaacataa ccatcggcaa gaataatgtg cctagaacga agccggcaaa tgctccacca
     1741 acgccaagta cccagttgat cccaccgacc atgccatcag aaatgaaacc tgctaaaggc
     1801 atgatgaaga aaatttcaat cagaccgatg gcaatcaatg aaagagttgg tgtaacgatg
     1861 atatctaccg catcaggtac tactttatgc agttttttct caacgattga caatagccaa
     1921 acagcgaaga tgacacctag gatcccacct tgtccagctg caagagcttc accagtaaat
     1981 aagtttttca gtggtgcttc cggattcatc cctgtcagca tcacaacggc cccgataact
     2041 ccaccaagag ttggtgttgc accaaaaact tgtgcagcat tgatacctgt atagataact
     2101 aggtagctaa acatcccgtt tttcaagatg ttcatcacat cgatatattg ttgccatgtt
     2161 gctgcatcaa gtgttccggc tgtgactaag tttgctaaaa ctgcggcaat ccctgccacg
     2221 atcccagtac cgacaaatgc aggaatcatt ggcacaaaga tattagaaat gtcttttaat
     2281 accgatttga ttttagatgg tttttgtttc gatttttgtg ccgctttcat ttcttgtgcc
     2341 cgttcgttta cacggtcttt tccagatgct gatgaatttg ctgtagctgt acttacagaa
     2401 gaaagttttt ctcctaattt cacgcctgcc aaatcaacca tttgctgtgc tactttgttc
     2461 actttcccag gtccgatgat gacttgaagc tgttcatctt ctactacacc aagcactcct
     2521 ggaatcgctt ttaattcaga gacatttaca agatcatcat tgatgatctt catacgcaca
     2581 cgagtcatgc agtggacaat gctttctaca ttgcccatgc cgcctgcccc tttataaatt
     2641 tgttttgcca gttcttccaa agtcagttcg ttttttgcca tggtcttttc cccctcataa
     2701 ggtccctttt acaaatccgt ttgcttcagc taattttgct tgagcttcct ctttatcact
     2761 atttgtcaaa atcatgacga tagccagctt aacattttga tctgcttctt caaagtactt
     2821 ttcagctgtt tcgtaatcag cttctgtcgc ttgcatgatg attcgttttg agcgttcgat
     2881 tagtttttca tttgttggtt tgacatcgac catcaagtta ttgaatactt tacctatacc
     2941 aatcatactg atagtagaaa tcatattcaa gataagtttt tgtgctgttc ctgacttcaa
     3001 tcgagtagag cctgtcaaaa attctggacc tgcatcgact tcgattggga aatccgcgtg
     3061 tttactgatt tcggcttgtt tattgcaaga aatcgttcct gtgacagcac cgatcgattt
     3121 tgcgtaatct aatccaccga tcacatatgg tgtacgaccg ctagctgcga taccaataac
     3181 tatatcattt tttgtcaaat tcagcttgat tagatcttct tgccctagct cataagaatc
     3241 ttcggcccct tcgacagcta cagtcattgc ttcttctcca ccagcaatta gacctatgac
     3301 catttccggt tctacaccga atgtcggtac gcattcagca gcatctaaca cacctagtct
     3361 tccacttgtt ccggctccca tataaatcag acgtccacct tttttaaagg actcaatcgt
     3421 gttagcaatc actggttcga tacttgtcag ttctttttca accgcagcgg ccactttctg
     3481 atcttctttg ttcattagtt gaacggcttg agaaatactc atctcatcta atccaaaagt
     3541 ttcttggttt ctgcgttccg ttgttaggtt ctctaaattg attttgctca tttttatctt
     3601 ccttcttttc ttataaattg atagttcata ccagctttga tttggttgat caatggtagc
     3661 tcttctttga taactttagc tactacattc actttttcat ccgctggcaa atcatatttg
     3721 gttaattgga tctcacccat atagcgcaaa tacttttcgt tatctaaagt aatagaccct
     3781 ttcatacgcg tagctgtatt tcttgctggg ataggcggaa tctctcgaaa acgtgcatct
     3841 acgcttcgta tgacatctct tgcatcgtct tgccgattcg tatgcttccc taatatatat
     3901 tcgtaaaatt cttcatctac aggttccgta tggagcaaga ctgtctgttc tttttggaaa
     3961 gaagcaaatt gctcctgaac gttttctgac agacctgcat ctccgatgta aacaatatcc
     4021 gtgttacttg ctaatagatc tagagcgcct gcaagaggat ggatccctcg atgttcttcc
     4081 aaagttggca atccttgata tagtgggcct ctcaactctg tatctcctgg tacaaaacct
     4141 tggacaagga gaccttgaat tttgagccat tgattttttt tctgatacca ttctttgtcc
     4201 agccctgttt cgggacgtgg ataataatta tgccaagctt caatctgtga gaaatcagct
     4261 tctgcttctt tcaattcatc gatatcttta ggcgtgatcg tgctagcatt caaactgatt
     4321 ttcaattgat gtgaccagtc ggcaatctgc tgattggaaa tatgataatc cattcttaaa
     4381 cctgttacac caattgcttt caaaggcgtt agatcttcta atgaaaaacc cgctcgttcc
     4441 aatgcctctc ctgaaatatc gaccatcaat tctagtttat tctttttcgc ttcgcttcct
     4501 agttcgatca atcgcttttg atatgccgaa acatcatctt ctggtatatg catggaagtg
     4561 aaaataccga caaagccatt ggatgccatt tggcggatat actgtttctt ctcttctgac
     4621 aaagaatcgt tcataaatat tgaaaagcca aacatattat ccctcttttt ctgtatccgt
     4681 cttcatttct atgactagag tatatatcta aagaaacaaa atttcaatac ttttattaaa
     4741 ataattaaat ttcatttcta aaggttgagt ttatattatc aaaagaattg tcataaccta
     4801 aaaaaggcac tgctaagcaa aagcagtgcc ttgaaatatt ttatttaaat aacacaaaaa
     4861 cataattact cataagaaac caattctgta ttatctacca tgaatgtaaa gaacccctca
     4921 ttaggcatcg gatgaaaata agcattgatc actcttatca aggcattatg gcaaactaac
     4981 agatagacat tttcttcgtc ttcgatgcat tctttcaata aaggaacgat ccgtgcatac
     5041 acatctaaga ctgactcccc atttgggaat cgtacagcga acgctaaacg tgctttttga
     5101 aagttctcat ctttgcttgg cagtccatca taatccccaa aatccatttc taccaatcgt
     5161 tcatcaacgg tcagcggcaa gctgagacga tcagcaactg cttgtgctgt atctcttgct
     5221 cgttgaagag gggaatggat gattttcgtg attggaacag gcaaatctac taccttttct
     5281 gctagtttct gtgcctgcat ctgtcctttt tcagtcagcg gcacatctgc ccgtccgcaa
     5341 actaatcctt gtgcgttcca agttgtttct ccgtgtctcg ttacataaag catgtgattc
     5401 tctccttcgt tggcaaagtt ccccttaacc ataccttgtt gttcttgaga ttacaagggt
     5461 tagattctct caagagaaat aatctcagta gatactttat ccaggaaggt ttgatcatgt
     5521 tccactagta acatggcagg tttcacgttc aagatcagtt gttctaattg ttcttgattg
     5581 aagacatcca aataattcaa tggttcatcc catgtatata gttcagctgg ctgtgataaa
     5641 gatttagcca attccacttt tttccgttgg cccatactca tctgctcgat cttgttatga
     5701 aaaacatctc tttccatccc aagctttcgg aggttgttca aaaatgcttg gtagtctact
     5761 tggtttttct ctgcaaattc cgccaacgtt cctcgattgt cttcataatt ttggcttgca
     5821 taactaatgc tcagatgttt tggctggtat ttttctccta taaccttgcc gttgaatgcc
     5881 cccagaagat aatggataat ggatgacttt cctgcaccgt taggacctga aatcgccacc
     5941 cgctgatgag gctcgattgt aaaatgaatt ggctcgaata acagattttc ataccctaat
     6001 tgaagatctt ctacgcttaa aagtcgctta tggtgagacg cttggctatt catcgtcaaa
     6061 gaatcgatat attcgatatc ttttaatagt ttttctttct cgctgatctg ggtctccatc
     6121 cgattcacga ttgctttgga tcgtttcatc gtccgtgcag catcagcacc cactgctcct
     6181 ttattcactc gtctagattc agtatcgatg aatccgactt gtttctttgt tttatctcct
     6241 tctcgggaac gagaccattc ggctttttca gctgctgttt tctttagcct actgacttct
     6301 tttttcaatt tttcattttg agccatttca aattcatcac gaagtttttt ctgttcttca
     6361 tagatagaga aattcccttg ataaagttcc agttgacttt tttcgattgc taaaacatgg
     6421 tccactactt catcgataaa tccccggtca tggctgacca cgatgaagcc ttgttttttc
     6481 tttttcaaat aagccgctac ttgttttcta ccagagatat ccaaatgatt cgttggttca
     6541 tcgattaacg ggaaatgagt gtcatccaca aataaaagtg ccagtaggac ttttgtcttc
     6601 tctccccccg atagtgtgct gaattctctc cataagattt caggatctgt ttgcatcaat
     6661 tggagctctc tttcgatttc ccatatctca aaatccgtaa tatcatttaa cacgtaatag
     6721 gttaaacgtt ctttatcttt tgtctgttgc gggaaatagg caaattcttg ctgatggatt
     6781 acttgccctt gataaggtag tttgttttgt agaatattca gtaaagttgt ctttcctcga
     6841 ccgtttcgtc cgataagtcc tagtttccat tgtgtgtcaa aatttagatt tgcttgttca
     6901 aataataatg tgccttggct gtcgtagccg aatgtcagat tttttatttc gattttagac
     6961 attttccatc acctcgtaaa aaaagaccag tcctattttc agaactggct aaagaggatg
     7021 caagggttgg atacacacaa aaagacccat acatcgaaaa aaccaagcca atcccgaaaa
     7081 tctgcacact gcttccttgt ctccacaagg tacagtctga atgcagcttt catcaatcga
     7141 cttaattttt caatgcacgg atccctcact ttgtcctata tttgaaatta cgatagcata
     7201 aagaaaatga ttttgtcaac tacttcggga ggatggtgaa aaatctgcta agtgtctgtc
     7261 ctaagatttt tgttttttct aatgtttgcc tgtgttccat tgcttcttct aaacctattg
     7321 gacccaaatg aatacttaag acaattggga gaaaatcaga aagtccttct agctcttggt
     7381 ctctacttcc gcaaatagcg attattggtt ttcgatattt ttttgcaagc actgctacac
     7441 cgtatggaac ttttccgtgg atcgtttgct gatcgatccg cccttctcct gtaatgatga
     7501 catctgcttg ttcgatcttt ttttccaatc cagtgatttg gctgactaaa tcgaaaccag
     7561 aaaccatctt cgctcccaac aaaaaaaggg ctccaccaat accgcctgct gctccacttc
     7621 ctggaatatc atctaaatcc acctcccaga aatcttttat ttgctgagca atctttcctg
     7681 cttgtttatc taagaaagct agcgtcgtat ctattcctcc tttttgtctt ccaaattgcc
     7741 aagcagctcc ttgttgacct gtatatggat tggtaacatc tgcagcgata atcaatttta
     7801 ctttagaaac aagaacgttt cttcgatcaa aatgaagttt tttagtagat aataaaggat
     7861 ttccttctgt gtacccttct aaagaagcac ccaaggattg gagtaatcct agaccaccat
     7921 cgctacaccc gcttcctcct aaagtgacaa taatttgttc tacttcttga agcagtgcat
     7981 ctttaataag ttctcctaag gcataactgg aagcaagtcg gatagtttga tccgttggtt
     8041 tatcaagtag atctaacccc aatgcttgtg cagattcgat gactgcggtg ttttttcctt
     8101 gccaagttgt caataaatag ctgatttttc gctgacggaa taagagatcc atcccatcaa
     8161 tggtacgcca tgttcccctg agcgcgtaat agattgcttc tgcagtattt tctccgccat
     8221 ctgcaatcgg cacattgatt tttcttcctt tacgatcttc ccagccattt agaactgcct
     8281 gatttagttc aaatgaagaa gcacttcctt taaatgaatc aattgccgcc aaaatatcca
     8341 tctgtatccc tcactttctt cttaagtaag acactttttt tcattttttt caaacaaaac
     8401 aatgctattt tcttccattt ttctatatac taaaagggaa ctcattgaat aaaggagaga
     8461 aaaaacatga aaatcgcaat agctggggcc ggagcaatgg gcagccgaat cggattgatg
     8521 ctacatcaaa gcggaaatga ggtcctcttg atcgatcgtt ggcctgctca cattgaagcc
     8581 attcgtacaa atgggttgat tgctgacttt aacggtaaag aagtggtcgc aaaactgcca
     8641 atctattcac cagaagaaat catcgaaagc aacgaacacg tggacttgat cgtagcatta
     8701 acaaaagcca atcaattaga tgatatgttt tgttcgatcc aatccattat tactgacaat
     8761 acttatgttt tgtgtttact aaatggatta ggtcatgaag atgtactgga aaaatatgtg
     8821 ccaaagaaaa atatcttatt tgggatcacg atgtggacag ctggtctagc tggtcccgga
     8881 aaagtcacat tgttaggtga tggagaaatc gaactagaga acttggaacc agaaggcgaa
     8941 gcatttacaa aaaaagttgt agaagtcttt caagaagcaa atttaaatcc gatctatagc
     9001 cataacgttc gttattccat ttggcgaaaa gcttgtgtaa atggaacatt gaatggttta
     9061 tgcacgattt tggattgtaa tattgctgaa ctaggcgcgc aaaaagcggc agagagcatg
     9121 gttcgaacaa tcgtatctga atttgcttct atcgcagcaa aagaaggaat catactagat
     9181 caagaagaag tgtaccaaca tattgcttct acttatgatc ctgacaacat cgggttgcat
     9241 tacccatcta tgtaccaaga cctgatcaaa aaccatcgtt tgactgaaat cgactacatc
     9301 aatggtgcca tttggcgaaa aggacaaaaa tatgatattg ctacaccata ttgtgccttt
     9361 ttgacccaat tagtccatgc aaaagaagga atccttggcg cagaataaaa ggctctctga
     9421 tgagaatcag ctgaatcagc agctggttct ctttttttaa gcccaataaa agatcaaaaa
     9481 gaaaacacaa ctctcatgat gccataagaa ttgtgcttgt ttgtttcagt acataatctc
     9541 ttcaaaatgt aggtttttta tttgttttca acagaggaga ggtcatattc gtattgctca
     9601 taaatagtgg aatcgttagt gataaatcgt tttgcccaat tttctgcatc ataaaattcc
     9661 aatagctctg ataggattgg cttaaatggt gtatgcagaa attccggtaa ttcatctaaa
     9721 tcacgttctc gaagcaaaaa aatattttca ggacgataaa aatgataatg agcaatcgct
     9781 tgattatccg tcactagttt cttttgaaaa tacatggatt caaatgtccg caaagtcact
     9841 ccttgctgac cctcccgcaa cagttccaga atcccgcgcg attcccctgt ttttttcagg
     9901 tactcttcat aagtaagata tccaggttgt tcattccctc gatgtggaag aatgaaatag
     9961 ttggtcgtaa catctctttt tataaattcc tgcttatagc tttctgctcg ttcttttctc
    10021 ccattattcg ttgcaccaaa aaatagatcc gtctttggtt cgctagaaga aaaattcata
    10081 tgtttcgaat aaaatgaact attatgtttc agcccaagtt cttttgcttc caaaaaatca
    10141 aaatgataaa tctcatcgat tgccggttca tcttgtgcct gcttcagcat cgcctcgtgt
    10201 tctgctttga agtgattcca aaaaaataac acaatttttg cattggtaaa tgggcgcacg
    10261 cgtcgagcca tctccatatc aaatccataa tctaaaaaaa cgatcagctg ataatttgcc
    10321 aactgttgtc tagctgattt aatgaattcc gactgtagta aatcttttcc ggaccaaaca
    10381 tctaaagagc ggatggaagg caaaaaataa agtgatttat tttttgcaac tagcagtacg
    10441 tcatttttgt tcatcttttc cgcctcactt tctctgtcac tttttcctat gatagcctgt
    10501 ttaaatagta aaaagccagt aaaacgtttc ctatatttgt atgaaaattt aacatttgta
    10561 tgaaaatatg atgaaataaa tcattcttct attaaaattc gcctaaaagt tttctgaata
    10621 gttgtaattg tctgaaaatt tgttatcatt agtcattgtt aaatattgct atttattctt
    10681 aatataaatt ttgattgtga atttaaaaga aagaggtcat ttatggaaga taaaaaattg
    10741 aaaaaacaac ttagctccag acatatcacc atgttagctt taggtggagc aatcggtgca
    10801 ggtctattta aaggaagtgg cgaagcgatt gggatcgccg gcccttctgt attgattgct
    10861 tttttgattg gtgggctgat tttatatatc gtgatgaaag gtctgggaaa aatcgtttta
    10921 agcggtggcg acacgcatca cggattatct ggtttgatac gcccttatct aggtgctcat
    10981 tctgctgatt ttactgattg ggtctactgg tctatgtgga ttatcaatat catcgctgaa
    11041 gctgttgcag ctgcttcctt tttgcagttg tggttcccgc acattccagc ctgggtattt
    11101 gtttttatgt tggctgtttt aactacgatc atcaatcttt attcggtacg gttattcgca
    11161 gaaacagaat attggctggc ttttgctaaa atcagtgtca ttatcttact gatcattttt
    11221 gctacttatc ttgtcggtca gcaaatgttg ggcacaggcg ttttccctac ccttcagcaa
    11281 ttgactgacc atggtgggtt tactcctcat ggtatgaagg gaatcatcag ttctttgctt
    11341 gtagttatct attcttacgg cggttctgaa ctgattgcta tcacggtaag cgaagcagat
    11401 gatcctaaac gtgcgattcc taaagcaatc aaaggtgtca tgggaagaat catttccttt
    11461 tacatcattc ctttgttttt gcttttgatt ctttttcctt ggaatacttt agcaggcacg
    11521 aatgtcagtc cttttgtgat ggtatttgaa aaaatgaata tcccatttgc agcggatatc
    11581 gtcaattttg tgattgtcct tgctttgttc tcatctatca attcgggtgt gtatgcatct
    11641 tcaagaattt tgttcttccg tttgaaggat cgaaaaggtt cctctagaaa actagctgtt
    11701 ttaaacaagc atcaagtacc gcaacgtgct gttttatttt gtaccagtgt tctttatctt
    11761 ggtgtagctc tttcttattt tgtcggtgac aaattatttg gctatctggc aggttccctt
    11821 tcgtatacag tattattgat ctggatcatt atcagtgcgg catcctttgt actggcacta
    11881 agaaaaggaa cattatggga taaaggaatc agcttttttg cattagctgt cttaggattg
    11941 attttccttg gtatcctgtt cacgaactcc attggggtaa ctgtattgac tggcctgctt
    12001 tacctgttca tttatttcag ttatcaaaaa aagaatgatg cgttcgtatt gacgaatgaa
    12061 tgaaatcgat agaaaatttt attttattta tgtggaatag atggagctat tgaagaaagg
    12121 agttgtgaca aaagtcttgt tgcaactcct ttttccgaat aaacagtctc atgacctctt
    12181 ggtaaacggt gttcaaaaac cctctgtgca aaataattga ttgttctcca tgctcatgac
    12241 atgatagaag aaaggaatga aaaaaaggag tttgttttaa atgaaaaagt attggaagtt
    12301 tttgttgatg atcggtgttt caacggtagt tatgtttggt atgatgtact tgaatgtcta
    12361 tgcgctcgat catatcttct tcagtcgtac acgagtattc atggcgttga tgatgggtgc
    12421 agtcatggca gtcatcatgc tgttgttcat gtggaaaatg tatgaaaaca aaaagataaa
    12481 tgttatgatc ttagccgcta gtcttcttgt ttttgccggt tccttgttta tggtacgaag
    12541 ccaaactact gtcaacgaca cagcttggat gaaggccatg attcctcatc attctatcgc
    12601 tatcctggca agtaaacgag ctgatttaaa agatcctgaa gtaaaaaaat tagcggaaga
    12661 aatcattcag gcacaagaag aagagatcga caaaatgaaa aaaatgatca aagaaatgga
    12721 atgaccatta tccgtccaaa taaaaaaaga ggatgttcca gaagttatca gcttcaagaa
    12781 ataagaagaa gctacttctg gaacgccgtg aataaggggt tgtgaaaaag ctgccctgca
    12841 tcaagtaata agaacagcca aacaaaaata gcttctccta tttttcgttt ggttaggctt
    12901 attaccgcag atgcaagctt ttgaacaccg tttattaaga ggttgtaaaa tcagcgttct
    12961 gacctgagta ttagagagac aaatggaaaa atagttcctc atattttgcc attttttgag
    13021 ctaataccga gggtcagctg attgaacacc gtttatcgag aggttgtgaa aaagtcgctt
    13081 agtttcgcgt attaagaaaa attttgtgaa atggctttcc acatttttgt ataaatttgg
    13141 cttaataccg agaaactggc ttttgaatac cgtttattcg taaaagggat tgtgacaaaa
    13201 gtcttgtcac aatccctttt tatgttttta ttgttctaat tgcgctgtct tgcttactgc
    13261 ttcttgcggt ttcacaaatt tttttgagat tccccaacct gtgaaggcac tgaccatcga
    13321 aaacacagga gaaagcaaac tgaaaaatac gaatggcaag taagtgagtg taggaatacc
    13381 aaatgtactt gctacgaaac ttccggcaat tccccaaggc actaagtagt tgattactgt
    13441 tccaccatct tctagcgttc tacctaatac agttggatca aatcctgctt tttcataagt
    13501 ttctttaaat gcattaccag gcagaataac agataggaat tgttcaccaa taaaaatatt
    13561 tactccgata cttgtcatca atgttgtaaa aatcaaattt ggtgtgttcg tcatctttct
    13621 tgatactcgt tccatcacgg ttgagatcaa gccaaattcg atcaacaatc cacctaatga
    13681 caaagttaga ataatcaatg acacagtagg catcatactt tcgattcctc cgcggctaag
    13741 cagtgaatcg atttgttggt ttccagtatt tgaaacaaac cctgattcga tgaccccagc
    13801 caacttcgtt aaggaagttt gtgggttttg aataaaaatc attcctactg ctacagcgat
    13861 attcaacaaa atcgttaaaa ttgccggcac ctttttccat gcacacagta acattaaaaa
    13921 taaaggaatc agtgaccaga ttgaaatagt aaagtttgct tctagaacat cagtcacttc
    13981 acgaatagat gaaagacttg cggctttact tgtgtgtcct aaaatcatga ataaaaagaa
    14041 agaaacaaca aatgccggga cagttgacca catcatatgc ttgatgtgtt tgaacaaatc
    14101 ggcatcaaca atagctgctg ctagatttgt tgattcggat aatggagaca tcttatctcc
    14161 aaaaacggca cctgaaacaa tagctccaac aaccagcgct ggatttaagc ctagtgttgt
    14221 cccaataccg aaaaatgcaa tcccaatcgt tgacataaca gtaaaggcac tccctacagc
    14281 gctacctacg attgcacaca caacaaatac agaagggaca aaccactgga cactgatcaa
    14341 tttgaaccca atgaccatta gagttggaat gatccctgct tgtatccata cagcaatcaa
    14401 tgaaccgact aaaataaaaa taaagattgg gatgattcct ggtttgatgc cattgatgat
    14461 tccttcgtgc agccaatctg tcgggtatct ttttatcact gcatacaaca tgaccattcc
    14521 gatcgctgct aagatcgtga tattaggtga gacctttaat ccgacaacgc caatcgcaat
    14581 gcctaaaaca ataaccatta atacaagtgt tgcttctaaa aaacttggtg ttcctttttt
    14641 tcccatgata atctctcctt ttttagttgt tcttatgttc taaaaaagtt aagatatcgg
    14701 attactccgc cgcaattcat ccgcgattcc tcctttataa aaataaaaag acctgtttta
    14761 aactaaggac gaatgactcc gcggtaccac cttaattaaa aacagatctg tttttcactt
    14821 tcaaactttt attcaaatgt atggactttc catgtaattc gtcaattagg cttgatcggt
    14881 tcacagcgac caccgacttt ctgaacaaaa cacctaaccg ctactgcgtg aaaagttgac
    14941 ttgtacgaag tttaccttcc ataagatcat ttgtcaatga aaaagaaaat attttttcat
    15001 ttttctgaaa attattttta taactttcac acacaagatt attttttact agaaagtagc
    15061 gccctcttgt ttttttcttg ctactatttc ttgatgttct tgcttgattc tttctaatag
    15121 ctttggctct ttcaacagat caagagctgt attggcgagt agttctgctg caatcgcaat
    15181 agaagcaagg cctttgtcac ttttagcagc agctttgaat tcttctgtgt gtccggcaat
    15241 atatgtctca gagattgcta ccgtaggctg gattgttggt atgacttggc tgacatttcc
    15301 tacatcagaa gaaccgagac ttgttctttg ttccgtttct atttcttcat ctggaacacc
    15361 tgcttctttt gcatgctgga agaataagtc atcaaaagaa ggagtaacga tgacatcatc
    15421 aactgcattt tggaacaatc caaactcaaa agtcgtgccc gtacttatcg cagcacctcg
    15481 tacgatattt tccacttttt catatacttt atctaatgtt tgccgatttc ctgcacgtaa
    15541 atagaaacgt ccagcagcgt aatctggaat gacgttggca gctactcctc cgtctgtaat
    15601 gatcccatgg atattgacat cttttggcaa ttgcaagcgt agagcgttga ttccattaaa
    15661 cacttggatc aatgcttcca aagcatttac tcctttttca ggtgctgctg ctgcatggga
    15721 agcgacaccg taaaatttga tgtcgactgg atcattcgct aaagattctg tcgttttccc
    15781 ataacgaaaa gctggatgga cgcataacgc tacatcgaca tcctcaaaaa aaccttctcg
    15841 aacaaagctg cctttagctg atccgttctc gcccccttct tcccctgggg tgccatatac
    15901 acggatttct ccaccagttt catcaatgaa ttgcttcact atgctggcgg ctaatacgga
    15961 gtaagtacca aacaaattat gcccgcatgc atgaccaata cctggtaaag catcgaattc
    16021 tgccaaaaag gcaattgtgg gaccgggttt attggaaata taacgtgcat caaaggctgt
    16081 tcggtgtcca gctacttctt tttctaccca aaacctttct ttttctaatt gtttaatcaa
    16141 cgctgctgag gaataaaact catagttact tacttcagga tgttcgtgga tatctagagc
    16201 aagtgcttga tattctggta aacgatattg gattagatcc ttcaattggt ttgttgagat
    16261 tgttgttgtc ataaaaaatt cctcctatgc tagtcatttt tcttattttc tcccgaatgt
    16321 ttcccatgca ggcacgcttg atccttttga tgttttctcg attactttga ctgtgtcttc
    16381 tgtttgataa gcgtctacta ttttttgata cgttttattg ttttcatctt cttttcttgc
    16441 aacgatgata ttcacataag gacgtgcatg gtcatccact ggttctaaga agatggcatc
    16501 agaagcagga ttaaatccag catctacagc tacaccgcta ttgatcactg aaatgtctac
    16561 atctgacaaa gcacgtgctg tttgggaagc atctaattca gaaatgtcta gattttttct
    16621 attctcggta atatcagaaa cagtgggcgc ttgtcctttt gccttatcca ctttgatcaa
    16681 tccagcagtt tgcaataaca gaagagctct gccgccattt gtaacatcat tagggattgc
    16741 tacttttgct ccgtctttga tatcagctac atccttcaaa gtgttagaat aaattcccag
    16801 cggtgcattg acagtatttc cgatcgatac taaatcagtt ccgtgttctt tattgtaatt
    16861 gtctaaaaag aattgatgtt ggaaagaatt caaatcaatc gatccatctg ctaaagccac
    16921 atttggttga ttgtaatctg taaatttcac gtattccaaa ttaatgttct cctttttcaa
    16981 acggtctttc acgttgtccc atacatctgt atcttctcca ataatcccta gttttacgat
    17041 agtttctttt ttgtcagttg tatctgcttt tccttggcta caccctgcca accaaataat
    17101 tgctacaaat gtaatgacta caaaacgaat aatttttttc attttttctc ctccactttc
    17161 aatgtgtaat ttttttaatc agttgattac tgataaattg acttaaaaaa actaagatta
    17221 gaatcaataa tgtagccaca atcgttacat ctgtttggaa tcgattatac cctctcgata
    17281 tagcaagatt ccctaatccg cctgcaccta ctgctccagc catggtagtc agtccgatca
    17341 cgttgatgat agtcaacgcc gagacccgaa tgataccagg caaaccttct aacaaataca
    17401 cacgtaaaat gataccgatt ggactggttc ccatcgcttc tgccgcttcg atcaccccag
    17461 gatcaacttc tagcagtgcg ttctcgattt gacgagcaaa aaacggaaca actccaaaaa
    17521 tcaatggcac taaagctgct ttcgcaccaa tcgtcgttcc aactaagaaa cgagtagtca
    17581 atgctaaaag agaaagcaag ataatgaaag ggatggaacg aatgatattg atcagcttat
    17641 caagtatttc aaatagcaga cggttagcta gtaatccatt agggcgtgtc actactaacg
    17701 tcacacctaa gatgattcca atcatcccgc caatgaatgc tgtccaaaaa gacatatata
    17761 gtgtttgctt cgtcgcttcg acaaattcat ccggaatcaa ataaacattg ggcaaatatg
    17821 ttttaaagaa atcagtcatt ttatcttcct ccttcgatca ctttgatacg actatctaaa
    17881 tcgttttttt gtaaaatatt tatttttaca ccagatttga tcaaataatc caatgctttt
    17941 ttccgctgtt tggtttctcc agctaaagta acgatcaaac tccctaacgg cgtttcttgt
    18001 aataactcta cattgccata taaaatattc gctgttactt gatatttgct gtacagatga
    18061 gcgattagag gttcgttcgt ctgatttcca atataactta attccaccag ccatttattg
    18121 gcgatctgat cggcaaaagc agaatgggca atgatcgttt ctaatgcttg gtcaagatga
    18181 gtagctgttc gaataaaatc tttggtcaat tcttcttcag gattgctgaa gatctggatg
    18241 ctgctacctt tttcaacgat tcttccatct tccataaccg ctaccttgtt gcagatttct
    18301 ttgacaactt gcatctcatg agtgatcagg acgattgtta aacctaactg gcgattcaat
    18361 ttttttaaca atgcaaggat ctgaagggtc gttttcggat caagtgcact ggttgcttca
    18421 tcacataata ataccttagg ttcattcgcc aaagctcgtg cgattgctac tcgttgtttt
    18481 tgtccccccg acagttgttt aggataagca gatgcttttt cttccaaacc gacaagttcc
    18541 agtagttcat tcactttttg acgacgttcc tgtttcgatt ttccagaata ctttaaagaa
    18601 aaatcaacgt tgtcaaaaat agttcggctt tccattagat tgaaatgctg aaagatcata
    18661 ccgatggcct ttctttcttt tcgcaattct ttgggagaga gctgactcag attcgtctga
    18721 ttgacaatga cttctcctgc tgttggtttc tgcagcaaat tcatcacacg aactaacgta
    18781 cttttccctg cacctgagta accgacgatt ccataaacat cgcctttttc aatcgttaaa
    18841 tctacagaat ctactgcctg tatctctttt tctttttgtt tgaaacatac agaaacatct
    18901 cgcaattcaa tcattaaaat tcctccttat ctaaaaaaag cgtcctcaag aaaatgaaaa
    18961 tgtcattttc ttgaggacgc ttttttgcgt gttaccacct catgttcaag ctgtgttcac
    19021 acacaactcc tcttcaagta cgatcatact ctagcaagat atcggtcgca gccgtcatcg
    19081 ccttacatct atgctcggga tgaattgctc gatggccatt ttccaatagt cgttccttgc
    19141 ttcttctcag ctaccgaagc tctctgtcaa aggttcttct atcgtactta ccatttcaat
    19201 gcaaataata ttcaaaaaaa aagtcctttt actaacataa actatgtcag taaaaggacg
    19261 acgtatcgcg gtaccacctt ttttcggaag tttccttccc tcaagcccaa catctcaaaa
    19321 agagagattg gcgttcgttt taacggtcga acagttccga atttgcctac ttatcgacaa
    19381 attattctcc aaggccattt tcagcttttc ttacttgact cttctcacca gccagagcct
    19441 ctctgcaaag tttccaaact tactttcctt ttcaacgaaa tcgatatttt gttgttggga
    19501 ttaatctaca tgaggaaaac tgaaaagtca agtacgaaat tcaaattatt ttttctatat
    19561 ttatttttct gtttttttat ttaacatttc aataacagtt cgaagatctt ttccttccgc
    19621 aagaagtttc aatttttcct tttcttcgac ggtataattt cggacaaaac tggaatcagg
    19681 tatcaaaaca acgcctaatc ctgctcgtga agcagctgtt gctcctacta aagaatcttc
    19741 cactgcaaag gctgaactag cttctaatcc tgttcgttcc aaagctgtca gataaggaat
    19801 cggtgaaggc ttgttctccg acacttcgtc tccgaatgta aacgtgtcca agtaatcagc
    19861 taatttaaag tgatccaaga tatctgttgc tcttttcgta acagtggtcg tagcaatgcc
    19921 tagtttcata tgacgttctt ttaagtctgc caatatttct tttgcatatg gttttaagtg
    19981 gatggctccg cttgtcagct gttgataaaa atattcttca cgcttcgctc ggacttcctg
    20041 tactttttca gggctacctg ctgctttcac taaaatatca aaactttgtt tccagttttt
    20101 tccgccccaa gcgtcaacga tttcttttgg gatatggata tcaaattgct gaaatcccca
    20161 aagccagcca tcacgatacg ttttttctgt gtcaactaac aaaccatcta aatcaaatat
    20221 tgcacctttc atctatacat taatcctttc caaaacaata cacatagttt agcacgactg
    20281 gtccaaaaag aacagaagca aagaaccagc cttctcgttt ataagagttc ttggaatcga
    20341 cggatataga ttttcttcac agctgtcaca agcgccagat aagcgatgac tgtagcaaat
    20401 aaccataacc agtaattgcc aggaagtggc atcaagccta attcagcacc aaaacttgta
    20461 aaaggaagga cagaaccgac aataattcct aatgtagtga taacaaacat ggcaaatgtt
    20521 gcgttgcttt ggataaatgg tactttcggt gtacgcagtg catggagcac taaggtttgt
    20581 gtccatagtg attcgacaaa ccatcctgca tggaataatg cgatgaaaat cactcgctgt
    20641 gaactatcca atgtatggaa acttccgccc actacagcag gacaaataac aaaatacatc
    20701 aacaagtaag tagtaatatc aaatatcgaa cttgttggac caaagtaaac catgaattcc
    20761 ccaatactag atgcattcca tttttttggc tcgtccaaat attctttatc catccgatcc
    20821 catggcaagg aaatgcagga aacatcatag atcagattca aaaacaaaag ttgtaaaggc
    20881 agcattggta aaaatggcag gaacgtactt gcaatcagca cggaaaacat attgccaaaa
    20941 ttagaacttg ctgtagcctt gatatatttc atgatattcc caaaagtttc tcgtccagaa
    21001 agaaggcctc tttccaaaat cgtcaaatct ttttctaaaa ggatcacgtc tgcagattct
    21061 ttcgcaatat ccacagctgt atctacagaa ataccgatat cagccacttt cattgcagga
    21121 gcgtcattga taccatctcc taaaaatccg acagtatgac catttttacg taaagctgtc
    21181 gtcaaacgtg ctttttgctg aggattcaat tttacgaata cttcatggtc ttcagctact
    21241 tgtgctaatt catgatcact catttccgag attttctcac cggtaaccaa ttcattgatc
    21301 tctagaccta cttgacggca cactgaacgt gtaactaatt cgttgtcccc agtcagaact
    21361 tttacagcta caccatgttc tttcagagct ttcaacgctt gtttcgttgt ttcttttggt
    21421 ggatcaagga aagcaagata accaatcaaa accatgtctg actcatcttt gactgaaaat
    21481 tctccaacga cgcttggatt ggtcttttgt gcgacaccaa tcactcgtaa gccatcttca
    21541 ttaagtgact gtacttttgc aagtacggct tcttttcgtt cttttgttaa tggtgtaaca
    21601 gtaccgccaa gatcaataaa tttagaaatg ttcagcattt cttcaatcgc accttttgtg
    21661 atcatttgtg ttttaccagc tggatcagca accacaacac tcatacgacg acgttcaaaa
    21721 tcaaaaggaa tttcatccac tttttgataa ttgatcttat ctgtatctaa agtttctgtt
    21781 gcttcatcaa tgatagccac atctaacaaa tttttcagcc ctgtttgata gtagctattt
    21841 agatacgcat ggcgcaaaac acgatcatct tcatttcctt cacaatccat atgatattca
    21901 agaatgattt tatcttgtgt caatgtcccc gttttatccg tacataacac atcgattgca
    21961 ccaaagttct ggatggcatt caaatttttg atcactgttc cttttttggc catcacactt
    22021 gctcctttga ccaaattcgt cgtaacgatc attggaagca tttcaggtgt tagaccaact
    22081 gctacagata gtccaaaaag aaaagcttct agccaatccc cttttgtaag cccattgatg
    22141 acgataactg ctggagccat caatgacata aatttgatca gtagcatgga tgttttatgg
    22201 atgcctaatt caaagcttga ttgaattggt ttttcagata gcgtttctgc tacatgacca
    22261 aatagtgttt catttccaac agaaacaatg attccttccg cacttccact gatgacatta
    22321 cttcccataa agacaagatt ttcgtaactt gtttcgcttt gctgatcgtc ttcacatact
    22381 ttcgcatgct tttctactgg atagctttcc cctgtcaatg ctgcttggga aatgaataaa
    22441 tcctttgcgg acaataaacg catatccgct ggtatcatat cccctgctga aagccgaaca
    22501 agatctccac agacgatttc ttccatagga atctctgtgt actttccatt acgttttaca
    22561 gctgccgtta ctttcacaag tgatttcagt ttttcagccg cttgattgga acgtacggac
    22621 tgaatcaacg tcattgtccc gctgacgata accatagtga agataatggc tacaccgaaa
    22681 agatctttat ctccaggagc tgctaataca tattccgtga tgaatgaaat aattcctaat
    22741 gcgatcaaca ctaatgtaaa tggcgtgatg tatgctttca atatttctac aatgaatggt
    22801 gtttttttgc catatgaaat cgcattttca ccgtaatttt ctcttgaacg ttcgatttgt
    22861 tcttctgtca atccatttgc tgatgcatgg aatagttcaa ataattcttg tttcgttttt
    22921 ccagcaaaag ctgcatagat tttttgtgta tctttctttt tttccatctc aatctctcct
    22981 ttgtgtccaa aagacagctg ttctcgctgc ttttggtctt tagctattca tcggatcgat
    23041 agagaaaaaa ctgaaagggg ctgagaacag atagataata gccctggcct ttcctctaga
    23101 tccttgtttt tcgaatgact gtcatccatt tgttctcatc tcctttacta agtaatttta
    23161 attcatgact tccaatcatt tttcgaatag ttatttttcc aattagacca cctccttttt
    23221 atttttcacc gatggggatt tgcggcaaca aaaaaacctc tcgccaaata ctcagcgaaa
    23281 ggtttgaaaa agcatattaa aaaaagcttc tcagatctca tcgttgagtt ttgacactgt
    23341 acaacgtaaa aagcagatac ctgcttttag ctaccttatg aaaagcctta tctcgacaat
    23401 tcctgttcta cccattggcg tctctcgacg tttttgggca gtagcctatg tttgtatagg
    23461 agcctcacct aacaaatctt attcggtttt ccttcgttaa tataacaact aaaaaagagg
    23521 atgtcaaatc aattttccat gtttttcata ctttttttaa aatacacacg ttatattcct
    23581 gtctcacgcg gttttatttg tctcattttc cacctcccgc tataatagaa atatagtagg
    23641 aaacgaggtg ctgttatgga aagaacactt gttattatca agccagacgg cgtgagacgc
    23701 catttagtcg gaagcatcat ccaacgtttt gaagcaaagg gactggccat tgctgaaatg
    23761 aagtttgaaa ctatgactcc tgaattagca aaagaacatt atcagcactt gactgagcgc
    23821 agtttttttg atgaactgat cgattacatg acttccggtc ctgtcgttta tcttgtgctg
    23881 gtcggcgagg aagtgatcga tatcgtccgt aagatggttg gagcaacaaa agctgccgat
    23941 gctgttccag gtacgatccg aggagactac gcgctgccgg ggactgaaaa catcattcac
    24001 gcttctgatt cccgtgatgc tgcggtaaaa gagatcgccc gctttttccc gccattcgat
    24061 ataaacaaac cgtctgaaaa agcggcgaag tgatacaaat tctaaactgc tggtctagtt
    24121 gctgggttca gcaatggcca gcagtttttt ctctaaggac ctccgttgat tctttcctaa
    24181 catatattcg agcactgtga agaattcttg gtagtctttc gtcttttctt cccctaaagc
    24241 agcagaaatc aatctttgat aataatctgc catcaatgtt tcatagatca ctgtacttct
    24301 ttctttatgt gctaagctcc gtttcatatc ttcattcaca ctacgtgcct ttttcaaccg
    24361 accattttct atataaaacg aagtcaggtt cattaaaaaa gtaaagagat actgctcatg
    24421 attgttcaac tgtgctaatc gtcgcattct ttttttgatt ttcaaataat tgttctcaat
    24481 gaaccgatca ctgaatatcc aaggaaaatc agctagaata gatagttcaa attgaccata
    24541 gtctggtatc tgcatcaagt attcttcaat gattcggcat tcataacgta aaaattcaaa
    24601 ttctgtctca gatacttctg ttttttcgac catgtgaaga ttcaaaacca aataatacag
    24661 atcttctgtt ttcttaaaat gcctacggtt attgctgatc tctttataag gatcaaagct
    24721 gtcttcttgt acccgatcta gcattcgtct ttttttcttt aactttattg gttcatccag
    24781 ctggtttcgc atccattcat attctgcaag tgtcatgttc attcgctcta aaaatgatct
    24841 caatgttaca aatgacattt ttttcactga atttgtctca atcccgacta gagaagtctt
    24901 aaaacagatt ccttccgcta attgggactg tgtcaacttc ttgttgactc gaagtttctt
    24961 gatcaattca ccatcataca taatagttct cctttttaat ttaaattttt ccacattcaa
    25021 taaatagata cgttaaaaag gaaaaataca cgaaaaaaat aaaaaaatgt ttttttcata
    25081 aaaaatacca accttcaagc attagaatgt tgtctaacta ctgaaggttg gtttgattgt
    25141 ttattgatta tttttttgct gttcaacgtc tgttcttgtt actggatata agttggttgt
    25201 tgttttatta ccattttttg aaaagacact tgtcgaatgt tcgccttttt gcttttcgat
    25261 tgcttcatta cgttccgctt catttttata gtcatatttt ttcggatcaa tcgtctcaaa
    25321 tccgtttggc acataaaacc gcaagagatt ttcctgattc agcttatctg aaagacttaa
    25381 cgcagtcttg actttttcct gatctttgga gatttcctca tcgattcctg ctgctttcgt
    25441 atcgatgatt tctcctgtct gattattgta agcttttcct cctaggacgg tgtatttggg
    25501 tgtcacaaag ttcccatttc gaaaagcaac tacttgatca tgctgagaag ataataagtc
    25561 tgtaccaaaa tgaagataat ctttatcatc gattcccaat agatgtagta atgtcggcaa
    25621 aacatcgatt tctccaccgt attcatgatt gactgtccct ttagtataac caggcatatg
    25681 gatcatcaat ggcactcgtt gcatctgtgc gttgtcaaat tcatcccatg tatctggatc
    25741 tttaccaaga gcgcttgcta aatctttatt atctgtattc gagatcccaa aatgatcccc
    25801 gtaaatcaca aagattgaat tttggtaaat accagatttt ttcaaatacg taaaaaattg
    25861 ttccaatgat tgatccagat aatgtgccgt tcgaacataa ttatctactg tgctattgcc
    25921 agtattcaaa gatggaaagt cgaaattctt gtcatccgta taataaggcg tgtggttggt
    25981 tacggaaaga aatttcgtgt aaaacggctg ttgtagatgc tcaagatatt gggcagattc
    26041 tctgaataaa tctttatcta atatcccata acctaacgtt tcgtcggatt catcaaaata
    26101 agatcgatca aagaaattgt cataacctaa gtttttatat acatgatctc tattccaaaa
    26161 actagctaca ttcccatgga aaacagcgct tgtatagcct tgcttttgtc caaggatcgc
    26221 tggtgcggct tgaaagacat tgtctgaccc aagttctgtg aataatgagc cttgaggtaa
    26281 accaaatgtg ccagtttcca acatattttc agcatcactt gtctttccct gtcctacttc
    26341 atgaaagaaa ttgtcaaagc tgatcgttgc ttgattctgg aaaatactat ttaaaaacgg
    26401 tgtgacttct tgtccgtcta ccttcatatt aatcaaaaac tgctggaaac tttctaaatg
    26461 gagaacgatc acatttttcc ctttagcgat cccaaatgtt tcgggatttg gctctgcata
    26521 atgtttcttt gtataatcta aaacttcatc aatcccatta ctgcttgcat gcgcgcgaac
    26581 actgctagtc attcctgttt tgattccgtc ataaatagta tacgcatcga ttcctagata
    26641 tttgacaata tatgaacggt caaatgttcg ctgcagcaat tgtggacggt tggcttcgct
    26701 cagcgctaga tttcctgaaa aaataagccc tgaaagacag gtaacagcga tagccatcgg
    26761 tttgccgaca gggttgcttt tgataggtat tttcttgacc ttcaaccaaa ccagtagccc
    26821 aatgaacaca gcgatatcca gccagtagat gacatcatgg ggcttcatca aagaaaagga
    26881 gcttcctgac aatccttgcg acactttcga aaagcctaat acagatttga ctgtaatgaa
    26941 atctgtgaat tcccgataaa aaataatatt caagtacaac aaaagcgtat ttaaaatatc
    27001 aatgatcaaa agaatagcta acgctggctt tggcttttta atatacaaag aaaggccaaa
    27061 aaacaataag gtagtcgcaa ttgggttgat ccacaagata aagtactgga tcgtaccttc
    27121 cacacctaat gaaaaatctg catagtaggc aatgatcgtc ttcagccaga aaaagaaaac
    27181 agctgtcaaa acgattgcta aacgattttg gcacaatttt ttaagttgtg tcacgtatat
    27241 cacttctctc tattcacatt ttttcttatt ttgctcctga aaaatgaaaa tttcctaaaa
    27301 aagttgttat cttaagattt ttttattctt tctaaagatt attccgtcca cttttcacag
    27361 cataaaaccg ctgcgatgta agcgattaaa ctaatgaaga gataaaagtc ttgtggtgac
    27421 cgcttcactc cacggtattc aaaagccagt ttctcggtat tcgtacaaaa aaagaggctg
    27481 tgacaaaatt cttgtcgcag ctccttattt cttgatgcag gacagctttt tcacaacttc
    27541 ttccttaaac ggtgttctcc tgccaactcc tcggccacaa gtcacaatat ttataaaaca
    27601 tgagaagctg ttttttgaaa ttgttccttg tggctcagag ttaaacggca ggctcaagac
    27661 ctcttgatac acggtgttca atcagctgac cctcggtatt agattatgtc aggacgctaa
    27721 ttttacaacc tcttggtaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccaacc
    27781 aaacgaaaaa tataaggagc tattttttga aattgctcct tgtggctcag agttaaacgg
    27841 caggctcaag acctcttgat acacggtgtt caatcagctg accttcggta ttagctcaaa
    27901 aaacggcaaa atatgaggaa ctattttgcc gtttgtttct ctaatactca ggtcaaaacg
    27961 ctgatttcac aacctctgtt tttcattgtc cataggattg atattgtcct tgtgatgact
    28021 cattcgtaat aattgaggct tgattttctt cacttcccgt tatcgtttgt ccactgccag
    28081 aatctgaagg acttgttcct gtcgactcgt ttttttgaga agagacagtt gttttcttga
    28141 cttgttcatt tgtctttttc cattccgcta aacgatcggc gaccactttt ttgtctactt
    28201 tttcagaata agcggtgatc acttttccat ttttcaaacg ggctactttg tcgccatcat
    28261 gagtcaaaag caaaacggtc aaatgcttgt ctttaaattg atattgtctg agagaaatcg
    28321 cactaagtac gtcatcgata ttaataaatt gattttcttc ttttcctttt ggcaaataaa
    28381 tgtccaattc gcctttttcg taattggcag ttattttata ctccgcccac caataagttg
    28441 agaattcttt tttgaaatta tctactgatt ctttgcttgc tacgattttt tctgtttctt
    28501 tctgagcaag atctgctgtc atttttcctg aaaatgctgc ggataaaata agcagtaaga
    28561 aaaaaccgaa taaccaaaag agaagaaacg gtcttttttt cctcatgctt tcctccactc
    28621 ctatttaatt ttgactattc tattatatac aaaaaatgaa atagaaagac aaaaaagttg
    28681 aaaaagatcg taaataattg aacttctttt tcacaaaatg gtaagaatgg agaatttcac
    28741 gcatttgctc tcaactattc tttcttaagc ctcaaatccg tttttatctg atacgtcttt
    28801 gaaccaatga cccgattttt tgattgttct ttttccatct tgtgctaagt ctactgaaat
    28861 aaagccatat cgattcttat atgcattggt ccacgaccag ttatccatac atgtccacat
    28921 atgatagcct aaacaattgg caccttcttg aatggcttga tggacatatt tcagatgaga
    28981 ttggacaaat tcgatccgat aatcatcgtg gatcatgcca tcttctttga taaatcgtgc
    29041 ttcgttttct acacccatac cgttttcaga aataaagcag tcgatatttc cataatgatc
    29101 tcgtacattg atcaaaatat catagattcc ttgttcatag atctcccatc cgcgataagg
    29161 attcatttta cgaccaggca tttcataatt gtcgaaataa tcctcaggca ttggaccgtt
    29221 tttagtatcg attggtgttt cttttgcttt tacccggcgt ggctggtaat aattgatacc
    29281 taaaagatct actgtatttt ctttgataac cgtcaaatcc tctgcatgga tcacaggcat
    29341 gagatcaagt tcttttacta tctcaatcaa ttcacttgga aactcgcctt ttacagctgg
    29401 atcaaggaat gaacgattga agaacgcatc tgctatttgc gctgctttaa catcttcagg
    29461 gtttgattcg tctcttggat agcttggtgt cagattcaag atgataccaa taacaccacc
    29521 ttgctccatt tcatggtaag atttgatggc taacgctgaa gccaacgctt catgatagcc
    29581 aacttggatc gcttttttca tatctgactc ttctggataa tgccatcgat ataaataacc
    29641 tgcttcgact ggaacgattg gttcattatg agtaaaccat ttttttactt tcgaaccaaa
    29701 caattcaaaa caacttttgg catatttttc ataggcttca accgtttctc ggttcaacca
    29761 tccgcctttt tcttgcagat acatcggcat atcgaaatga tacagattca caaagggttc
    29821 gataccgttt tcaatcaact catcaaaata acgatgatag aaacgaacgg cttcttcgtt
    29881 gattgttcca gattcatcag gtatcaaacg gctccattgg atagatgttc gaaatgaatt
    29941 atgcccagtc tgtttcatca actgtacatc ctcttgatac ttcgtataaa cttgcgaggt
    30001 tttttcaggt cctacttgat caaagaactt ttgcggttct tttttgtacc aataatcaaa
    30061 aatgttctct ccttttccat cgcctgaaat ccttccttct gtttgtgggc cacttgctgc
    30121 agaaccccac caaaaatttg ctggaaattg atattttttc atcaaagcac ctctttttct
    30181 tttttagttc gttttagttg attccatgta tgatattttc catcacaatc actttttcat
    30241 tttcttcctc ttcttttttg attcgtttta acagctgttc tgctgctcgt tccccgattt
    30301 tatctgttgg tggcgtgatc gtgattttcg gaaggatact cagatcgttt ttatctaaat
    30361 aatcatagtg actgaacgca atatccttcc cgattctgac accttcttga ttgaaacatt
    30421 cgatggcacc aatcgacata ttgtaattgc tgacaaaaat agcagttgcc cctttttcta
    30481 ataatgcttt tgccccgtaa tagccgcttt ttctagaata gtctcctgta aagacctcta
    30541 attggatcga tggatcattt tggaccgctt ctttggctcc tgctaaacgt tctcttgcta
    30601 cgtagtctgt ttgagtggca gcaataaatc cgattttttt atgtccttgt tcaccatgat
    30661 gagcgatcac ttttgctaca ctttctcgat cattcactaa tattgtatca atacctgctg
    30721 gtccttctgg acaattcaat gcgataactg gaatactcat ttttgtgagc atttcaaatc
    30781 cagaccactc ttcttcggat aagaagacaa tcagcccatc gatcacatgc ttcatcaggt
    30841 attcaatttt ttgttcaatt ttttctggct gatcggcaaa cccgcttaat aataagctat
    30901 acccattttc ttcagctact ctttcgatcc ctgagacaac ttccgaccca aaacggctgg
    30961 aaatcccgtt tatcaaaagt ccaatagaga agcttcgatt attcttcaac cctttagcaa
    31021 tgatgttttc tttatagtcc aacgctgaaa tcgcttctgc tatttttttc atgttctttt
    31081 ccttcagttg ctgaccattc aagtaacggg aaactgttcc cactgaaaca ccagccatct
    31141 tagccacatc acggatcgat gccatacatt ctcactcctg ccagttacat ttgatttctc
    31201 tttcttgttg ttcgattatg atgaccgcat tctttgcata gtgttgcttg tcatcaatat
    31261 ccatataact aaagtcttca gtagagcaac ttcctctcac tattaattat acttaaacaa
    31321 gtatttgaac cggttcattt ttaatatagc atttttttaa aagattgcaa cacttt
//
LOCUS       DANKKG010000018        31357 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000018 DANKKG010000000
VERSION     DANKKG010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31357
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.018"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(<1..822)
                     /locus_tag="PW004_000707"
     CDS             complement(<1..822)
                     /locus_tag="PW004_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL2725700.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAI"
     gene            complement(1046..2986)
                     /gene="thrS"
                     /locus_tag="PW004_000708"
     CDS             complement(1046..2986)
                     /gene="thrS"
                     /locus_tag="PW004_000708"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HDL2725701.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3323..3502)
                     /locus_tag="PW004_000709"
     CDS             complement(3323..3502)
                     /locus_tag="PW004_000709"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HDL2725702.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3549..5372)
                     /gene="cadA"
                     /locus_tag="PW004_000710"
     CDS             complement(3549..5372)
                     /gene="cadA"
                     /locus_tag="PW004_000710"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HDL2725703.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5650..7929
                     /locus_tag="PW004_000711"
     CDS             5650..7929
                     /locus_tag="PW004_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2725704.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8022..8669)
                     /locus_tag="PW004_000712"
     CDS             complement(8022..8669)
                     /locus_tag="PW004_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725705.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8848..9567)
                     /locus_tag="PW004_000713"
     CDS             complement(8848..9567)
                     /locus_tag="PW004_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2725706.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9698..10849)
                     /locus_tag="PW004_000714"
     CDS             complement(9698..10849)
                     /locus_tag="PW004_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HDL2725707.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11066..11806)
                     /locus_tag="PW004_000715"
     CDS             complement(11066..11806)
                     /locus_tag="PW004_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL2725708.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11810..12133)
                     /gene="rsfS"
                     /locus_tag="PW004_000716"
     CDS             complement(11810..12133)
                     /gene="rsfS"
                     /locus_tag="PW004_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HDL2725709.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12190..12783)
                     /gene="yqeK"
                     /locus_tag="PW004_000717"
     CDS             complement(12190..12783)
                     /gene="yqeK"
                     /locus_tag="PW004_000717"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HDL2725710.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12767..13417)
                     /locus_tag="PW004_000718"
     CDS             complement(12767..13417)
                     /locus_tag="PW004_000718"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HDL2725711.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13427..13738)
                     /gene="yhbY"
                     /locus_tag="PW004_000719"
     CDS             complement(13427..13738)
                     /gene="yhbY"
                     /locus_tag="PW004_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HDL2725712.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13754..14863)
                     /gene="yqeH"
                     /locus_tag="PW004_000720"
     CDS             complement(13754..14863)
                     /gene="yqeH"
                     /locus_tag="PW004_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HDL2725713.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGYFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLDGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14860..15387)
                     /locus_tag="PW004_000721"
     CDS             complement(14860..15387)
                     /locus_tag="PW004_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HDL2725714.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15567..16352)
                     /locus_tag="PW004_000722"
     CDS             complement(15567..16352)
                     /locus_tag="PW004_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HDL2725715.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16345..17214)
                     /locus_tag="PW004_000723"
     CDS             complement(16345..17214)
                     /locus_tag="PW004_000723"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HDL2725716.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17215..18594)
                     /locus_tag="PW004_000724"
     CDS             complement(17215..18594)
                     /locus_tag="PW004_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDL2725717.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18602..19021)
                     /gene="fabZ"
                     /locus_tag="PW004_000725"
     CDS             complement(18602..19021)
                     /gene="fabZ"
                     /locus_tag="PW004_000725"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDL2725718.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19046..19522)
                     /gene="accB"
                     /locus_tag="PW004_000726"
     CDS             complement(19046..19522)
                     /gene="accB"
                     /locus_tag="PW004_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HDL2725719.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19525..20760)
                     /gene="fabF"
                     /locus_tag="PW004_000727"
     CDS             complement(19525..20760)
                     /gene="fabF"
                     /locus_tag="PW004_000727"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HDL2725720.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20779..21516)
                     /gene="fabG"
                     /locus_tag="PW004_000728"
     CDS             complement(20779..21516)
                     /gene="fabG"
                     /locus_tag="PW004_000728"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HDL2725721.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21528..22445)
                     /gene="fabD"
                     /locus_tag="PW004_000729"
     CDS             complement(21528..22445)
                     /gene="fabD"
                     /locus_tag="PW004_000729"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDL2725722.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22552..22776)
                     /locus_tag="PW004_000730"
     CDS             complement(22552..22776)
                     /locus_tag="PW004_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDL2725723.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22829..23791)
                     /locus_tag="PW004_000731"
     CDS             complement(22829..23791)
                     /locus_tag="PW004_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HDL2725724.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23801..24241)
                     /locus_tag="PW004_000732"
     CDS             complement(23801..24241)
                     /locus_tag="PW004_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL2725725.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24547..25512
                     /locus_tag="PW004_000733"
     CDS             24547..25512
                     /locus_tag="PW004_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDL2725726.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(25569..25799)
                     /locus_tag="PW004_000734"
     CDS             complement(25569..25799)
                     /locus_tag="PW004_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HDL2725727.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(25885..26772)
                     /locus_tag="PW004_000735"
     CDS             complement(25885..26772)
                     /locus_tag="PW004_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDL2725728.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(26861..28084)
                     /locus_tag="PW004_000736"
     CDS             complement(26861..28084)
                     /locus_tag="PW004_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDL2725729.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(28101..29147)
                     /locus_tag="PW004_000737"
     CDS             complement(28101..29147)
                     /locus_tag="PW004_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDL2725730.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIRSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(29311..31038)
                     /gene="ptsP"
                     /locus_tag="PW004_000738"
     CDS             complement(29311..31038)
                     /gene="ptsP"
                     /locus_tag="PW004_000738"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDL2725731.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(31038..31304)
                     /locus_tag="PW004_000739"
     CDS             complement(31038..31304)
                     /locus_tag="PW004_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HDL2725732.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
BASE COUNT         8644 a         6886 c         5414 g        10413 t
ORIGIN      
        1 gattgcttct cgtgtttttt tcactttttc gctaagcttt tcgtttttag ccatatagaa
       61 ataaaaaatg atatcgatcg tagcaaattg ggccaaaagt gaattagttg ccgcacttcg
      121 attcgtgatt tcttttggtc tagatgtttg tacgagtaca tcggctatct tgctaagcgg
      181 gttatttcca aattgcgtca acgcaatgat ttcgatattc atgcttttcg ccagctcggc
      241 taatgcgact acatctgcac ttctacctga gtttgaaaca agccaaagca ctttcttttt
      301 ctcattgctg acaagttgcg ggagcaagac gtggatatct ttttcaaaaa tgattggctt
      361 accgacacga gaccattttt gcagaatatc ttctactact aaagaagagg cgccaacccc
      421 atataaaaaa acagtctctg ccgattccaa acactctacg actttttgga tcgtttcttc
      481 ttctagaata tctactgttt cttttatcgc tacttgtgca ttgttaccaa gtttgttttt
      541 gatagagtaa aaggactcat ttgcttctat atcactaaaa ccttcatcga ttccagtctg
      601 tagttctgtt gaaatcgtga ttttgaagtc cgtcagactt tgaaaaccga tttttttgga
      661 aaaacgtaca actgtcggcg cactcaaccc ggaagcttct gctaattcat tagctgtcat
      721 tgtagggacc ttctcaatat taccgataat gtagttggcc aacttttttt ctgctgaaga
      781 ataatcttcc atcatacttt caatacgact aattacgctc ataagttcct ccaccctttt
      841 aatgatagtc tatgtaagta taccatatca gacccttatt cacggtcgta tactggataa
      901 aaaacaaaaa aacaatccgc ccaacagaat atcttatccc atcaatccca cagattctat
      961 tgagcatctg tgtctttgag gaatacctgt tatctattgt tcggattgct ttctatctac
     1021 tactttttta caggaaattt acctattagt tttctctgct gtaatttttg acttctgctt
     1081 ccatagcatc aataaagata ttcaaatctt ctacagctgt ttctttactt ccataacgac
     1141 gtacgttcac tgttgcatct tccaattcct tgtctccaac aactaattgg taagggatct
     1201 tttgtgtttg agaggcacgt atcttatagc ccattttttc attacgatca tcaacttcta
     1261 cacgcaaccc cttcatttgc aaacgttctt tgatttcata agcataatcg ccatgtgcat
     1321 caacagaaac agggatgatt gttgcttgta taggtgccaa ccatgttgga aaagcgcctt
     1381 tgtatacttc tgttaaataa gcgacaaaac gttccattgt agaaacaatt ccgcggtgga
     1441 taactactgg acgatgcgta ttttccccgt cttctccaac atatgtcaaa tcaaagcgct
     1501 ctggcaataa gaagtctaat tgtatagttg ataatgtttc ttctgtacca agagctgttt
     1561 tgacttgaac atctagtttt ggtccgtaaa aggctgcctc gccttctgct tcaaagtaat
     1621 ctagttctaa ttcatccata gcggctttca gcattgcttg ggctttttcc cacattgcgt
     1681 catcatcaaa atatttatct gtattgtttg gatcgcgata gctcaagcgg aaacgataat
     1741 ctgtaatatt gaagtcagca tatactgcca ccatcaattc aagcgtacgt ttaaattcat
     1801 ctttgatttg atcaggacga acaaatgtat gaccatcatt caacgtcatt tctcgtacac
     1861 gttgtaaacc tgacaaagca cctgattttt catagcgatg catcattcct aattcagcga
     1921 tacgaattgg taattcacga taagaatgga tcgtgttttt gtagaccatc atgtgatgag
     1981 ggcagttcat tgggcgaaga acaagcattt cgccatctcc catatccatt ggcgggaaca
     2041 tgtcttcttg ataatgatcc cagtgtccag atgttttgta caactcaaca tctcccatta
     2101 ttggtgtata tacatgttgg taacctagac taacttcttt gtctacgata taacgttcga
     2161 tcgtgcgtcg gattgttgcc ccttttggaa gccagaatgg caaaccagaa cctacttctg
     2221 gggaaaccat gaacaagtca agttcttttc ctagtttacg atggtctctt tcttttgctt
     2281 cttcacggag acggatatat tctttcaaag ctttcttgtc aaaaaaggct gtcccgtata
     2341 cacgttgcat catttgattg tttgagtttc cacgccaata agcacctgcc actgataata
     2401 atttaaatac ttgtatacgg ccagtggatg gaacatgtgg tccacgacac aaatcgatga
     2461 attcgccttg ttgataagca gtaatcactt cttcttctgg caattcacta atcagttcta
     2521 ctttgtacgg atcacttgca aaaatttcta atgcttcctg ttttgaaaga acacggcgtt
     2581 cgatcggcag attttctttg acgattttca tcatttctgc ttcgattgcc ggcaaatctt
     2641 ctgcagttac ttggttttgt ccattatctg tatcgtagta aaaccctgaa tcgatagctg
     2701 gtccaacgcc aaagtggata tttggataga ggcgacgcat ggcttgggcc atcaaatgag
     2761 ctgctgaatg acggactaag ccaagtgcat cttcatggtc tggtgtgatg atctcaatac
     2821 ttgcatcttc ctcgattgga cgagtcaaat caacgagttt tccgttcact ttaccagcta
     2881 aagctttttt tgctaagctt tttgaaatgc tttcagcaat tgccaatgtt gttgttccag
     2941 actcaaattc tttgactgca ccatctggaa aagtaatttt tatcataata gtttcctcct
     3001 tgaaatgttt tgattgatca gatcatcaag ctgaaaaaca aaaaaatcct agcatctctt
     3061 gaaaagagat actaggacga aaagttcgtg gttccaccta atgttcaaac gcaaatgcgt
     3121 tcctcaagcg ttgtaacggt ccgaccgccc ttgcttcaac aagggtttcg aaagtggtca
     3181 tcctttggtt ttctcctgga aaagtttcag cctagctttt cctctcttat cgaagactct
     3241 caaattcagt tggctttctc atcaatcaat tatgtgttta tttaaccact aataaataga
     3301 aaaatcaagt gggaaactaa acttattttt tccattcccc atttacaccg tatgttcctt
     3361 ttttcatctc attgatgatg acatggatgt gttcttttgg tgcctgcgtg tttttatgaa
     3421 cagcttccgt gatatctttc atcatatttt ctaattgttc cggactgcga ccttctacta
     3481 attcgacatg tacgaatggc ataatgctct ctcctctttc tgatcgttct tttttcaact
     3541 cgattctatc atactatgtt tgtcttgggt actgaatcag gaaaaatggc tggatctttt
     3601 tttgctctca aggcactcaa aatagagacc gtatccacga cttcttgcaa aacagctccg
     3661 aataatgcgg ggattagtcc cgtacttgca attaacatca aaaaaataca aataaagatg
     3721 ccaatcagta cagactgttt cgctattctc atcgtatctc ttgaaatcac tactgctttt
     3781 gctacacgca agagatcatc tttcaagatg accgcatctg cactttcact agcagctgta
     3841 gaaccatgag cccccattgc aataccgata tctgcagcag caagtgctgg tgcgtcattc
     3901 acgccgtctc ctaccatcgc aacaggtctt tgatctttag ggatactttc taaaatggtc
     3961 agtttatctt gaggcaagct acgggcatgt acagaatcta attggacttt ttctgcaatc
     4021 gtttgagcaa tggcagatcc atccccggtg atcatcatca aatttttcac atcaagtttt
     4081 cttagctcct tgattgtctt tgctgcttct gggcgaatct gatccgaaaa taggatcgtt
     4141 cctgcaaaag atcgatcaat tgttacatag attgctgtat tggacgtttc tatttcttca
     4201 gctgaaacaa aagaggcacg tccaatcttt acttcatgat tattgactac tgcttggatt
     4261 ccttgaccgg tcacttcttt cagttgacta acaggataca gttggctgtt tttcacgttg
     4321 ttcactaacg aacgagctaa gatatggcta gattcctgtt ctgcactagc tgcatacaga
     4381 agtagctctt ctttttttat tccattgact ggctgaatcg cctctacttc caatatccct
     4441 ttggtaagcg ttcctgtttt atcaaaagca atcgtttttg tacgtgcgag cttttcaatc
     4501 gttgttccag tttttacaac gatcccgttt ttacttgaac ggctcattcc tgcaactaaa
     4561 gcaatgggcg cagctagaat cagtggacaa ggtgatgcca ctaccagtac ctcagcaaat
     4621 cgtaaagggt cttttgagaa ccaccaagaa ataccaccga tcaaataagc aatcaacgtg
     4681 aaagggactg cataacgatc agctaaacgg acaaatcggg caggcttttc ttttgattct
     4741 tcgactagtt taataatcat ttgatactgg ctgtctgcgg cacgtttttc agcgatcatt
     4801 tggatcgatc cgtctccatt taaagaccct gacataatct gatctccagg tttcttggtt
     4861 tccgggcggg actcacctgt caaagaagat tcatcgaccg aagattctcc tttggtcact
     4921 cgaccatctg cgggtacaag ttcgccaggc ttgactagta actggtcatc aatatttatt
     4981 tcatcgatta ctatatcctg caattttcct tctttgaatc gatgggcaat tcttggggtg
     5041 ttatctaaca aagactgcag ttctttaccc gctcgtttat tcgcgtaatc ctctagacta
     5101 tctccgcccg taagcatgat caatatcatc aaactagccc agtattcttt gacggctaat
     5161 gttgcaagga ttgcggtaat cgctaataca tctacgccat atcttcctga acgtaacgtc
     5221 ttgatcattt ccaaaaacat caagaaggcg agaatccctc cagtaaatgc gacaatcgca
     5281 aatgcaaggg ccggctggtg aaaaacaaat tcacttaata aagctatccc accaataagc
     5341 aaagtaagta aaaatttttt gaaatgtgtc attgtttttt ctcctctttt atgtgactta
     5401 gagaggttag taataaattt ccagctgcct ctttcatctt aagcatagtc cttgactagc
     5461 actttttaaa acaataggcc ttaattgaaa acaactatca ctatcagcgt ttccaaaatt
     5521 catcagttct acaaacaaaa atattcagca aggattactt tttcgattca tcagttaaaa
     5581 agtacctttt ttcgaaaaac tatgctatac ttttaattcg ttatttacgg cggaaggagc
     5641 aaacatgcga tgaatgagaa acaactagaa caaacgcacg tggatgaaac gatcgaacta
     5701 atccagatcg aacaaaatat gctggataca aaacaacaag aattgactca ggaaatgaat
     5761 cattcgacaa aagacacagc taatcacaag attcgagctg gttccaatga agctttttat
     5821 gaatctgctg ttgaatatcg ccaacatgag caggaattgc ttttgaaata ccatactctt
     5881 gaagcacagc aaaaacgcct gcaaacgttg tctgtcatga aagatagtcc ttattttgcc
     5941 cgtatcgact ttaaagaaga ccgtgaaaca gaaacattat atctaggtat cgcttcttta
     6001 agagatctgg cagaagaaac aatcgttatt gactggcgcg caccaattgc caatctttat
     6061 tatgaaggtg agattggtcc cgcattttat gaaactgaaa cagatagaat agatgtcgat
     6121 ctgcagttga aacgacaatt taaaatccaa gaaggacgta ttttatctat ggtagatacg
     6181 tcggaagtaa tcaatgatga ctttttgcta gagatattgg atgaatcatc aagcaaccat
     6241 atgaaaaaca tcgtctctac gatacaaaaa gcacaaaatg caatcattcg ggacacaaca
     6301 agtaaagtaa tgctgattga aggaatcgcc ggaagtggaa agacatccgc tttgcttcaa
     6361 cgtattgctt ttctgttgta tcataatcgc aagtggctcg aggctgaaaa cgttcttcta
     6421 ttttcaccca accatttgtt ttctgattac atctcgactg tattgccttc attaggtgaa
     6481 agcggcgtgc ctacacagac attcaaaaat tatttggtgc agctgctgcc agagttttct
     6541 ttactagatg aacagcaaca agaaagtggt tttttgctag gggaaaaaga tccgatacaa
     6601 gtgatgaaaa gcggattgac cttagtcgat cagattgatc gctatatcca aggcatcaca
     6661 aactacggtc ccttgttccg tgatatgaaa ataaatggcc gtacgatcct atcaaaggaa
     6721 tcgattcgcc aatggtataa agaaacaaat gaacaattac ctttacatca tagactttct
     6781 ctattacaaa cgaagctcct gaaaaaatta ggcggtctgc aaaaagacga aacacgtcag
     6841 caatgggtca aagatctggc ggaagaacag ctccaagaac tatacgcaac agatccaaat
     6901 cttgaatata cagaacaaaa ggagcgttca ttacgtaaaa aactggccaa ccaaatcgta
     6961 cgtaaaagat tccgtcgaat ccatcgaggg atcatgaatt atcaatttgt caatataacg
     7021 aaacaatatc tccactttct acaaactgtg ccactttctc ttctaacacg acataaaatc
     7081 agcgagacca ggtggcagga acatttgaca actgttcgta cacacttgcg tcaaagagaa
     7141 ttgcgtcaag aggatgctgt actattcttt ttactgatgc gccgaacata tccagtgacg
     7201 gttacacaca aagctcgcta tatctttatt gatgaaatgc aggatttcgc accatcacaa
     7261 gtggcattgc tgcaagcctt gttcccaaaa gcaaatttga ctttctgtgg agatctaaat
     7321 caaaatgtct ttggaaatga aacgatcgtt ggatcattgg aagacctttt cgtggaccgt
     7381 gaagtcactc gtttccaatt aacgacaagc tatcgctcca caaaagaaat cactgatttt
     7441 gctaatcatt ttattgtgaa agaacatcaa gtagaaacaa cagcaagaaa aggagacctt
     7501 cctttgctca tccaaggaac aacagatgat atcaaattga attggttagc agataccatt
     7561 cgaacgactc agggaaaagc ccggtattgg cgtacagcaa tcatagctaa aacacaagaa
     7621 gaatgcgaag ctctttatgc tcaactgcca gacgagttga aacaacaagt ccagctgatt
     7681 ttaaatgaga gtgactttat gaagcgttcc attattttac ttccagcctt tttagcaaaa
     7741 ggtctggagt ttgaccgagt tttcttgtgg gatgtagatg cagaacattt ttcttctcag
     7801 caagacaaat tgatccttta caccatgtgt acacgtgcga tgcatgaatt gacccttttt
     7861 acctccaata atgaaagtcc tttgttaaac aatatggata tttcatctta tgagaaaaaa
     7921 gtactgtaag taaaaagaca actgctatca ttaatgttag cagttgtctt tttatttaca
     7981 ataataattg cagaaaaagt aattcaatca tttaaggtca tttattctat atataaatcc
     8041 aaatctgttt caggatattt taatttgaca agcgaaataa tcattttcag ttcttccatg
     8101 ttttctggat attctgggtc aaataccttg ctatgacggc caatcccgcc attgtcgatc
     8161 aatcggtaaa ttcgattcca ttcaatgaat tggtcaattg attggaaatt gctgattgga
     8221 tagccgccac aatccaaaat ctctgataca cgaaatgcta ggtatgcact atcgatcact
     8281 ttattttttt tcgtgtctat cttctcattt ctttcgataa gaattttttc aggacgatgt
     8341 ttcaagctcc actctaatag tagaaagaaa gcctccatcc gatactcttc ttcaaataac
     8401 cgaaaggctg aatcagaacg acgaaaaagc agcggaatca gataattttg tctataaatc
     8461 agcattggtg cgcggtccac catacactct aaataacggt tcagataccc tgtaccgaca
     8521 tagtttccta atcgatagcc ttcaacgccg aatcttgcgt atttttgcgc taattttgaa
     8581 caatcagtta cgttcacagc taactcttct actttgcggt aaccgcttaa gatacgttca
     8641 aaaggttcta aagtgatttt tgcttccata ttcttccccg tccatcttcc acatacttca
     8701 tatcatttca catgatactt atgctctcat cataccacag gttgaattag aaaagaaaat
     8761 aaatcacaca aaaaagcaag acaatctgtt agtgagattg tcttgctttt cgaaaaagac
     8821 tgaatacttt tcgttaaaac tggttgctta gtcttctaat gccacattat ggaacacatg
     8881 ctgtacatct tctagatctt ccagtgcatc aatcattttt tcaaatgttt cttgatcttc
     8941 tggagatagt gttacctcgt tttgagggat catttggatc tcagcgattg aaaaatcagt
     9001 gattcctttt tcttttaatg cttcttgtat actatggaaa tccgctactt caccgtaaac
     9061 gatgatttgt ccttcttctt ccattacatc gcgaacatcg atgtcttttt ccattaggta
     9121 ttcaaggatc tcgtcagcgt cttctcctgc aaaagcaaag attgctgtat tgtcaaacat
     9181 gtaagaaacc gctccggaaa cacccatgtt cccgccattt ttcccaaatg ctgcccgtac
     9241 gtctgctgct gttcgattca cattgttcgt caatgtatca acaatcacca ttgagccgct
     9301 tggtccaaat ccttcatagc gcaattcaga gtatgtttca tctcctgcac cttttgcttt
     9361 ttcgattgct cggtcaataa tatgttttgg tacattataa gttttagcgc gatcaatgac
     9421 gaaacgtagt ttttggtttg catgcggatc aggatcacct gattttgctg ccgcgtagat
     9481 ttcaatccca aattttgcat aaatccggct attattagca tctttagcag ttttctttgc
     9541 tacgatattt gcccatttac gtcccatatt ttcacttcgc tttctgattt gatagtttca
     9601 taacgcaaaa acgtcctgtt ccattatact tgtgaatcac gattctgtga agcttttttt
     9661 ctttcttcct tcattcttct catttttagt caattatcta tactcggatt gggatttgcc
     9721 cgaaattctg ttccggaata ttgcctccca attgatagat ttgatcactt ctaactaaca
     9781 gttccgcttc ttgtgcctct ttttttccga ttcgggaata aatagaaatc tctttttttt
     9841 cttttaagaa tcttcttcca gcatcggtaa atccaagtag tcgaagataa tttttatttt
     9901 gttggacagg aatctcttgc tttttgatat tcagcaagac ataacaagat aatcgttgga
     9961 tccgtgtcca tgtatatctt tttgttttta cctttgacac aaaatcctca aaactatcag
    10021 cagttgcagc tgctgttttc atgcgcgatt ctattccttc ggtcatctga tagatatctg
    10081 tcagttccag caaagtagaa gataacaacc gataacgcaa atacggccaa taagattccc
    10141 aagtctgcag cgaacaagtt gaaagatctt cttctgtctg aggcggcaca agtccagcta
    10201 ttctttttcc ttcttctaac gcatggcgaa tggctgttgc actggctatc ctctgatctg
    10261 tgatgatttt ttcatgatag cctgcacttt tacgtcggat cggatgtaat tccatagggt
    10321 ggacatactt ggcattttcc tttgcataac ttaatcccaa gatatgattt ggcgaagaaa
    10381 aatcaagcgt aatatcggga tacagactgc gaaaaacctc agtcattttt tgcggataac
    10441 tatacgtttg atcagacagt tcttgaaatg cctcttctat cctatcttga ttctcttgga
    10501 caaagcgtcc gaaactgctg taatcaaacg aagtctcttc ttctgttcca aaacacaaac
    10561 tgtcacagtc cagtgtttgc aagattttta ctgcaccttt agcaaaatag tcagctgact
    10621 gaaccgccca ttcgacggga agctccacta ctaaatccac accattttgt aaagcttcat
    10681 tcgcgcgatg ccatttatcg atgatagccg gttcgcctct ttgcaaaaaa ttcccgctca
    10741 ttacagcaat gacgacctct gctcctgtta tttttcgtgc catttccaca tgatagcgat
    10801 gtccattgtg aaaaggattg tactctacga taatcccgca gcttttcatg cgcatcctcc
    10861 ttcatagtta tcgataaaaa agaaatagag acaagtaaaa aaaggcaaaa atcggaaaaa
    10921 atacccgaac tatataaact attctcgcaa acacaatact tctgcgacga aaagctgcac
    10981 tgcaagagca atgcctatat cgttcggata tataatttat gttgaagatt tattcttttc
    11041 ttgttaactc gagcaggcaa aaccgctagt ctttgtatgc tacaaagaac cagcgctttg
    11101 atttctctga tgctgcttcg tctgtaaaat cagaaaatgc ttcgactttc gaaaagccgc
    11161 tgttttccag cattcttaaa taactttcca atggataggt acgctcttga tgtagttcat
    11221 cttcacgaat aaatcgacca gacccttctt gttcttcaac gaaaaaggta agaaaatgtt
    11281 cgatgctgtg tttctcgctt cctggataac tatcccacaa aaaagcaaaa ttatcccctt
    11341 gataatgata gctatattct ggaaaaacct catctacttg ataggtggag tgtacgtcaa
    11401 ataaaaatcg gccattttct tctaacgctt gatagacttc atcaaacact tgctgtacct
    11461 cttgtctatt tttcatatag cacagcgaat cagaaaaaca ggtaattgcc tgatattgtc
    11521 cgatttctga taaatcgagc atattgcctt ccacaaattg gatcgatgcg tcttcttcat
    11581 atgctcgctg gctggcaatc atcaacatct cttctgaaag gtctaaccct gtcactttat
    11641 atccatcttt tgcaaaggca accgctaatg ctccagtacc gcatgccagt tccaaaatat
    11701 tttttgtgtc ttcttgtaaa tgacgtttcg aaaaagctaa ccattgctga taaagtgttt
    11761 cgtccatcac ttcatcgtaa atgaaagcaa atgtttcata agccatatgt taatccaacc
    11821 attcactaag atcgactagc ggagcatctg accatagttt ttccagatta tagaaatttc
    11881 gttctggcgc atggaaaaca tgtacgatca catctccaag atcgatcaat acccattttc
    11941 cgccttcttt tccttcaata cgttttactt catatttgtt ttcttcttct ttatcaatga
    12001 tttcttctgt aattgcgttg atttgacgtt cgctatttgc tgaacagatc atgaaataat
    12061 ctgccaacaa ggaaacattt cgaacgtcta aagcgataat gtcttctgcc cgttttgaat
    12121 ctgctgcttc aatcgcaatt tttaattgtt ctttactatc tatgatgatt tcctcctaat
    12181 atgagcagct tatttttttg ctacccagta attatatgtt tcgattgttt ttggataaat
    12241 ctgttgttct tgcgcaagca aatgggccaa tgtatgtttt gtttcaaatg ctacagcttc
    12301 gtctagatca gcccatgcaa tggctcgtgc atcttgtacc ccaggaaaat ctcgacctgg
    12361 ttcaatatag tctgctacat aaatgatttt gtcaaggaga ctcatcttag cagccccagt
    12421 cgtatgcacc cggattgcat gaagaatctc tgcatcggtg attcctaatt cccgctctac
    12481 aaacgaagcg ccaacaagac catgccagat tgcattatta taattcaata actccggatc
    12541 aaaaccgtct cttacgatta ccattttgaa ctcctcatct ggtcgttctt ttgcataatc
    12601 atgtgtcaat gcagcaatac tagctttttc aggagaagca ccgtattttt tggcaagagc
    12661 cacggctgtt tcttctactc caagcacatg cttgaagcgt tgttcgctca ttctcatctg
    12721 tattttttgc atcaggtttt ctctagaata agcagtatat tcattactat agtccatatt
    12781 catacaaccc ttcattttga atataatcaa ttactttatc tggaacaagg tagcggatag
    12841 aacagccttc tttgatcttt tgacgtattt tcgtggaact gatgtcgatc tcaggtacat
    12901 ctacccaaat tacaggatat ggagtatccg ttgtgtatcc tggtcgacgt atacccacaa
    12961 agttgaccat agaagtcagc tcatcgattt tataccattt tggcaaatat tctaccatat
    13021 ccccaccgat aataaagtaa taatctgtat ccggattgtt ttgcgtcaat tccttcatag
    13081 tatcataggt gtaacttttc ccaccacgag ctaattcaat cgtttcaatc tgcaaaaaag
    13141 gattgtcttc caccgctaat tccaacatgt tgagtcgatg ttttgcatca atcgtctgtt
    13201 tctcatcaac atgaggtggc tgataagaag gcatcaaaaa tacgcggtca agacctaaat
    13261 tccggccagc ttgttcagcc atcactaaat gtgcaaggtg aacgggattg aatgttccgc
    13321 ccaagatccc tacttgcttt ttcttctcta agaattgagg catttcttca ggaaataatt
    13381 gtgaacgaac aaatgtctta gcttgcgtat tcatcatggt cactccttat attgctttta
    13441 cttcttttga tagcctttga tacttttctt ttgtggaagg tttgtataat acaataacac
    13501 gtccaatgat ttggacgatt tcgcaatcta tttctttttc taatacttga gctgcttctt
    13561 ctgcaatctc atctgtattt tgaagtaacg atactttgat cagttctctt ttttctaaag
    13621 cttcttcaat ttgaacgatc attgctgaat tgatcccacc ttttccgatt tgaaaaatcg
    13681 gctgaagatg atgcgctttg ctacgtaaaa aacgcttttg ttttcctctt aaactcataa
    13741 ttttccttct tccttaaatc aatgctttgc gtttcaatac atctacaccc tttggcgccc
    13801 agcccgcaat tactactggc gctgttacag tgatccagcc tagaccggca aaaacgatgt
    13861 ctgttttttc tttcacagaa aactcaaaac gaacaagctc cggaaatgcc tcgacttcat
    13921 caggacgagg aggctgtaat aatccaccta catgtttttg ataaaattcg tctgcttttt
    13981 ccagtttcgt tcgatgaata tttacatcat ttgacacata agcaataaat gaagcacgat
    14041 tgccgctgat aaaatcaaat cttgcaagtc cgtccaagaa aagtgtttgc ccttcattca
    14101 gctgataaac tttaggtttg atttctttta acggtgcaat gatccgcaaa tcttttttac
    14161 ctaagtagtg cgccatttga tggcgatgga taattcccgg tgtatcaatc aaaaaatacc
    14221 cgtcatctaa aggaatctcg attttatcca aggtcgttcc tggaaactgc gaagttgtga
    14281 ttacatcttg tactcccgct gtatttttga tgatttgatt gataagcgtc gattttccga
    14341 catttgttac tccgacgata tagacatccc gtccatcgcg gtattcttcg attttttcta
    14401 atagttccgt catctcatga gatttcttag cactagttaa taatacatct actggacgaa
    14461 ttcctgcttc gtaagcacgt tccttcatcc attgtgttaa tttagttttt ttcaatgatt
    14521 taggtaatat atccactttg ttcccaacta aaagtactgg attgtcaccg acaaaacggt
    14581 gtagtcctgg gattagtgaa ccattgaagt caaaaatatc caccacattg acgatcaatg
    14641 catctttttg ccctaattca ttcagtaagc gcaaaaagtc gtcatccgtc aatgagacat
    14701 cttgggtctc attataatgg cgtaatctga aacaacgctg acaatagact tccccggaag
    14761 ctaaaccttt ttccaaagcc gtttgcggcg tgtatcctaa ttcaccgggt ttgtttgttt
    14821 gtataacggc accacagccg atacattgta attcttctgt cattcgattc cacctcgcca
    14881 gcccatatca gggtgttttg ctaatagata ggccataatt cttttttccc aaaaacgatt
    14941 aaattgtgtc ttccaactat ctgttgcgat aatgggtttt acaaggatgc tacgtatccc
    15001 tgcacgattg gctccacgta tatcagtcat aatctgatcg cctaccatta ctatctcatc
    15061 atctgctaaa tccaattgtt tctttgctat gttgatccct cttgctaaag gtttcattgc
    15121 tcgtgcaaca tacaaaagat caaatttttc aatcgctcgc gcgacacgtt ttgaattatt
    15181 gtttgaaaca acaacaacag ggattcctgc atttttcatt tctaaaatcc aagtaagcaa
    15241 ctcttctgtt ccatctggat tattccatgc aatcaacgta ttgtctaaat cggtcaatac
    15301 tgctttgatg cctagattct ttaattgggc aggtgtaatc tgatagatgg cttctaccat
    15361 ccatgttggt ttgtaatttg aaaacatgct gtctcctcat ctgcaaaaaa tgaaggagca
    15421 cataaagtac tcctcaaccc cgtattatac tgcatatcag caaaaaaaac tacctctata
    15481 ctcgttcgtc tttcagaaaa agcatttctt tacaaaatga ccaaaaataa agattgatca
    15541 cagaaaaatt tagaaacaaa aaacaactaa tattggcgga acctcttgta tcgtttttcc
    15601 agtaatttat gctgatctaa tcgaacaagt tccgtcaatc tttcaatcaa cgcttttttc
    15661 ataatgtgtg cgactttata tcgtggcaac tgctcgccgt tgaacgtttc aggaataact
    15721 cgatcaacga ttcctaactc gcgtagttct ggcgcagtaa ttttcatcaa ctcagctgct
    15781 tctgcggcgc gactaccgtc tttccacaaa atcgacgcaa atccttctgg agaaagcaca
    15841 gcataaatcg tttcttccag catccagact tcatccgcta aacctagagc aagggcaccg
    15901 ccacttccac cttcaccaat gatgatggcg atgatcggta cctttaaatc agacatttcg
    15961 agcagatttc tggcaatagc ctctccttcg ccgcgctctt ctgctcctac accgcaatat
    16021 gcacctgctg tattgatcaa ggtaatcacc ggtctattga acttttccgc ttgtttcatc
    16081 agtcgtaaag cttttcgata gccttcagga tggggagaac caaaatttct gcggatattt
    16141 tcctgcagat cccgtccttt ttgaataccg atcactgtta ccggtttgcc atctaatcga
    16201 gcgattccac caacaatcgc ttcgtcatct gcaaataaac gatcaccatg gcattcctcg
    16261 aaagaatcga agatctcttc aaaaaaatcc aagcttgtcc atcgttcttt agctcttgct
    16321 aactgaacga catcatgtgc tgtcttatcc acgatctatc catcctttct gtgtatgcaa
    16381 ttcaagaagc gtatggattt tctgtttcaa ttccatgcgg ggaacaatct gatcgacaaa
    16441 accatgagat aataagaatt cagccttttg gaaatcttct ggaagttcct gcttgatcgt
    16501 ctgttcgatg acgcgtcttc cagcaaaacc gatcaagctt tggggttctg ctagaataat
    16561 atcgccttcc attgcaaaac tggcagttac tcctccggtc gtcgggtccg ttaaaacagt
    16621 tagataaaaa agaccttcat tgctatgccg tttgacagca gcagagattt ttgccatctg
    16681 cattagagaa aagattcctt cttgcatccg tgcgccacct gaagctgtga acaagacgac
    16741 tggaagactt tcctttgttg ctcgttcgaa taatctggtg atcttttctc cgacaatcgt
    16801 tcccatactt cccatgataa aattaggatc catcactcca ataatgaagg gaatcccctg
    16861 aatggttgct ttaccagtga ggactgcttc atgaagtcct gttttatctt gcattttttt
    16921 aattttttcc gtatattcgg gaaatgctag cggatctttg gttacaagat ccgtatccca
    16981 ttcttcaaaa cttttttcat caataatcaa agctagacgc tgccatgcac cgattcgaaa
    17041 agcgtatccg caatgagggc atactttttc ttcaccgatg tctttcgtat aaaggatatg
    17101 tttgcaattt ggacactttg cccacatgtt atcaggaaca gatggcgagt tagcaggttt
    17161 gctctctcta ttaggattga tccgaatata tttctttttc ttaaacagag ccatctatgc
    17221 gcctcctatc tccgtttctg gtgtccaatt cggtaaaaat tcttcttgta aaaatgctgt
    17281 gctgtaatca cctgctatga ctgcaggatg actaatcaaa tccatctgaa attctgcgtt
    17341 cgtgatgatt ccttctgtga taaattcact taaggctcgc tgcatcttca ttaatgcttc
    17401 aaaacgcgta tttccatgga cgattatttt tgcaatcatt gaatcataat atggcgggat
    17461 cgtatatccc gggtaaactg cactgtctac tcgcagtccc atcccacctg ctggaagcag
    17521 taagttttgg atcttccctg gagaaggagc aaaatggaaa gcaggatttt ctgcattaat
    17581 ccggcattcg attgcgtgtc cttggaatac tacttcttct tgacacacat tcaaaggctc
    17641 accggctgct atctccacct gtttttttac taaatcgatt cctgtgacca tttctgttac
    17701 tggatgttcc acttggattc gagtattcat ttccatgaaa taaaaatcgc cttcttcatc
    17761 catcagaaac tcgatcgttc cggcattttc ataattgact gctttagctg ctcgaacagc
    17821 agcgtctcct agtgcttctc tttttgtttg tgagatcact acagaaggag attcttccag
    17881 tactttttga ttgttgcgtt gtaaagaaca atctcgctcg cctaaatgaa tgacatgacc
    17941 aaatcgatca cctaagatct gaacttctat atggcgagca ggataaatga ttttttccaa
    18001 atacatatca tcatttccaa atgccgctgc tgcttcttgc tgagctgaag aaaaatgctg
    18061 tgggagctct tcttttgatt gaactttccg aatgcctttc ccgccaccac ctgcagctgc
    18121 tttcagcatc actggataac caattctgtc tgctatttcc aatgcttctt ctactgtaga
    18181 taatacacca gtacttcctg gaatgactgg gacatttgct tcttgcatca attgacgcgc
    18241 attgatctta tttcccattg catcgatcgt tgcactttta ggaccaataa atgtgatatt
    18301 gcactcttca cacatggagg caaatcggct attttcagat aaaaaaccaa atcctggatg
    18361 aattgcttcg gcttttgtca cgattgcagc gctcagtact tcctgaacat tcagatatga
    18421 atcaacggct tttgccgggc caatgcagat tgcttcatct gctaactgtg tatgaagtgc
    18481 ttcggcatct gcttctgaat atatcgctac ggtttggatc cctagttcgc ggcatgcacg
    18541 aatgatacgt acagcgattt caccgcggtt agctatcaat actttagaaa acatatttca
    18601 cctatccaat cataaaggtt aattccgctt cggctacttt tttaccgtct actttagcga
    18661 ttcctttgcc tattccggct gaagctttca ctttcaatat ctcaacttca aggtataagg
    18721 tatctcccgg tgtaactttc ttacggaatt ttgctttgtc caatccgccg aaataagccg
    18781 tctttccctt gaattgttca agagaaagta atgcaactgc tcctgcttga gccattgctt
    18841 caacaatcaa tacccctggc ataacaggtt cttctggaaa atgtccttga aaaaagggct
    18901 cattgatcgt tacatttttc ttagcaacga tccgttctcc ttcaaccatt tcttccactc
    18961 gatcaatcaa aagcattgga taacgatgtg gaatgatttc ttttatttct tgtatgttca
    19021 ttgttttccc tctttcctgc tttgcttatt tttgctgaat cctaaagagt ggctgtccat
    19081 attcgacaac tgactcgttt tctacaagga tttctgtgat cactccgtcg gcctcactag
    19141 ttatctcatt catcagtttc atcgcttcaa taatgcagat aacttctcct ttggcaactt
    19201 gatcacctac ttttttgaaa gctggtttgt cgggcgctgg ttgcagataa acgattccca
    19261 cgattggcga aacaacctcg attccatcaa atactgccga ttctttttct ttcattattt
    19321 ctggagcagt tgttgcttgt tcaggtatga cattgactgg ctccgaaact ggcaacgttt
    19381 cgtttgtttg aaccatattc atgcctcgac ttgttttatt tttattcata tacaattcaa
    19441 attgaccttc acgcaaatcg aattccgtta gcgaggattg atcaaattga ctgactaatt
    19501 ctctgatttc attgatatcc aaaattattc ctcccagcgt ttcatgcaca gaaccgcatt
    19561 atgtccgcca aaccctaatg aattactcat tgcataagta aaacttgcag gtttgctttc
    19621 gtttttgaca actgtcaagt caatctcagg atcttgtttt tctacattga tcgtaggtgg
    19681 gataaattga tgttctaatg ccagcaaagt tgccaaggct tcgatcccgc cggctgctcc
    19741 caataaatgt ccagtcatcg atttcgtgct gctgacgtaa gtatctctga aattttcacc
    19801 taatgcgatt tggattgctt ttgcttctgc ctggtcattg gctggtgtac ttgttccgtg
    19861 agcattgata tatccgactt gttccggctg tacaccagct tcttccatcg caagcttcat
    19921 tgctttagat gcaccagaac catctggagt aggagctgtc atatggtaag cgtcacagtt
    19981 tgcaccgtat ccaacgattt ctccatagat tttggcaccg cgagcttgtg catgttctaa
    20041 agattcaaga actagaacgc ctgctccttc acccatcaca aaaccattcc gattttcgtc
    20101 aaacggaatc gaaccacgat taggatcagt tgattctgag agtgctgtta aagctgcaaa
    20161 tccagcaatc cccatctcac aaatcgttgc ttctgttcct ccagcaagaa tgacatcaga
    20221 atagccatgt ttgatgttac ggaatgcttc cccaattgaa ttcgttccgc tagcacaagc
    20281 tgttacgata gctgtacaaa taccttttgc acctacacgc aaagcaatat ttccagctgc
    20341 catgttggca atcgccattg gtacgaacat tggagaaact cgttttgcac ctttttcatg
    20401 tagacgaata acttggtttt cgattgttgg aagaccaccg atcccagatc cgatgatgac
    20461 gccaaaccgg tcttgattca ttttttcttt atctaaaccg ctcatctcca ttgcttctaa
    20521 agcagcgtag attccataga tcgaaaacat atccattcgt ttgctatctt ttttaataaa
    20581 atatttatcg taagggaaat ctttgacttc acccgctaag gtaatccctg tttctgaagc
    20641 atcaaattta gtgatcggtg cgatgccatt cgtcccctct tttaagctat tcaaaaagct
    20701 gtctgcgtca tttccaatag gtgaagtgac tccatagcca gtaattacca ctcgattcat
    20761 ttgtttgctc tccttttctt atccgtgcat gaccaatccg ccatcgacat tgatgacttg
    20821 tccggtgata tatggatttt gtgctaagaa aactgctgtt tgtgcaatat cttctacttg
    20881 tccgaaacgc tgcatcggaa tttgcttttc cgcttgttcc ttgactttgt ctgctaaaac
    20941 ttccgtcata tcagttgtga tgaatcctgg cgcaatggca ttacatgtaa tgcctctagt
    21001 tgcagcttct cgtgccacag attttgttaa tcctactacg ccagctttac tagctgcata
    21061 atttgcttga ccaatatttc caatcaaccc agatacacta gagagattaa tgatggcccc
    21121 ttctcgctgc ttcatcattt ttttcaatac gtgctgcgtc atattgaagg ttccaatcaa
    21181 attgatatcc agacattttt tgaaatcttc tgcatccatt cgcatcacaa gtttgtcatt
    21241 tgtgattccg gcattgttta ctaacacatg gatcgatcca agcttttctt ctgcttcttt
    21301 gatcatttgt cccgctttct cataatcaga gatgtcaccg gaaactccta cgcattttac
    21361 accgaatgct tcgatttctt ctatcttttc ttttggaatc tctccgcgac cattcaaaat
    21421 gatatttgct ccagcttttg caaaagctaa ggccatcgct ttgccgattc ctcttgtact
    21481 acctgtgata aatacatttt ttccttttac atccataacg ttcctcctta tagtattgct
    21541 attgtttcat caaatgtctg cttattctca acacgcagaa ctggtattgt tttatcgatt
    21601 tttttcataa atccgcttaa tacttttccg gggccgactt cgatgacttg ttcgatgccg
    21661 attgttttta acttatggat actctcatag aaacgaacag gttgcatgac ttgctgttca
    21721 agtagtcccg cgattgtttc tttcttcatt atttccgttg ttgtgttgct tatgatagga
    21781 aaagaaggtt cagagaattg aatctgactt aggtccttcg ccagtttttt tgctgccggt
    21841 tctaaaatag ctgtatgaaa aggcccgctg acatttagag ggatcattct tttgaaccct
    21901 ttttctttta atagtaaaac agcttcgtct actgcttttt cttcgccacc aatcacgatc
    21961 tgctgaggtg tattataatt tgcaggagat acgatgccgt acttgctagc ttcatgacag
    22021 ctttcctcga tcgtttcaat cggcgcattc atcactgcaa ccattttccc gcttccagct
    22081 ggggcagctt ctgtcatata agcgcctctt tttgctacta acgcaactgc ttcagtgaag
    22141 gatagcgcat tactagctac taatgccgaa tattctccta aactcagtcc taaagctgca
    22201 tctgggatga ttccatgctc cttcaataag cgataatacg cgatgctgac tgttagaata
    22261 gctggctgtg tatattgcgt ctgatccaaa cgttcatttt ctgtaaagca aagttcagcc
    22321 atatcgtagc cgagaatctc acttgcttca tcaaatgtct gctttacaac ggcttcttca
    22381 tataaatctt ttcccatacc ttgatattgt gcaccttggc cactaaataa gaatgctgtt
    22441 ttcatgtttt tctccaatat atccagaata tatgtttgat tcttcctatt caaaaaggcc
    22501 gagacattcc tatttcagga aatgtcccag cccccaacct aattttaatg attatgcagt
    22561 ttgtttttct acgtaagtca ctaaatcttg aaccgttttg attccgtctt ctgtttcgat
    22621 tttcacgtca aattcatctt caatttcatt gatgatttgg aataaatcca agctgtcagc
    22681 ttctaagtct tcttggatgt cagtagtcaa tttgatttct tcagtttctt ttcctaattc
    22741 ttctgcgatg atttcttgaa ttttttcgaa taccataata atatattcct ccgatattgt
    22801 ttgcttttta tttttataat ttaacagtct acaactccat gagaatactt ccccatgtta
    22861 aacctccgcc atagcctgta aatattactt tctgctgttt tcctaaggtg atctttccac
    22921 tagaaactgc ttcatccaat aagataggga tgcttgcagc agatgtattc ccatatttat
    22981 ccatattggt caggaacttt tcttgcggca tctttacttt tcgggcaatt ttctcaataa
    23041 ttctgaggtt tgcctgatgc aaaagcagat aatcaactgg tttgtctttc atgacttctt
    23101 ggatattgtt tgcaacgtct cgtacggcaa aatcaaaaat atctctgcca gtcatttgca
    23161 aataatacga accttctgaa tcttctgaat agaacggatt tttattttgc tggtagccag
    23221 cggtcaatga gctacttctg gtaccgtcag ctgctaattt ttctttcagc aattttttct
    23281 gtggattcgc ttctaagaga atcccacctg ctccatcgcc aaacaacaca gcagtcgtac
    23341 gatcagacca atcgattagc ttagatagtg tttcaccacc aatcaccagt cccttagttg
    23401 agccgcagcg gatcattttt tcagccatgc ttaacgcata aacaaatcct gagcacgcag
    23461 cactgatatc aaaagcgata gcatgagttg ctcccaattt cccttgtact tgtgctgcta
    23521 cagatggtgt cgtgtaatcg ggcgacattg ttgctactaa aataaaatcc aaatctttag
    23581 ggtttagatt tttctgtttt atcaattttt ccgccactaa aatgcataaa tcagaagtct
    23641 cctgatttgt tacacatctt cgttcacgaa tacccgtcct tgacgaaatc cattcatcag
    23701 aggtatccat catcttggaa agatcattat tagtaataat cttttcagga aggacgctgg
    23761 ctgttgcagt aatcattcca aattgttcca ttaaatatcc tcacttatag tcttgcaaga
    23821 agtcgtgcaa gtttttcagt gcatccagca aggcatgctg ttcctccgta gccattccgt
    23881 ctaagatgtt cttgaccatt tcacgatgaa aatgctgatg gacccgaaat agtaactttc
    23941 cttttttcgt tagtcccaac tttacaacgc ggcggtcatc ttcactgcgg atacgttcga
    24001 catagccttt ttttactaac ttgttaatag cagtggtcaa ggtcccaaca gtaattgata
    24061 gttctttggc tacttctgaa gttgtttttt tcttgtacat cccgattgcc tcgatcgtgt
    24121 gcatctctgt gattgacaga tctttgaatt gagatttttt cagctcagac tcttcgattg
    24181 tcaaaatatc attgaagaca ctaacaagat aatcatttac tgtttttaaa tctggttcca
    24241 tgttccaccg tccactaatt agtttgatta tcaaatcttt tgatgttcaa actatatctt
    24301 aacttttttc aaaatgcaac cttaaaagat cattttttta ctatatgagc tttttttaag
    24361 attttactca tttaaaacaa ctgataaatc agcatttata atttctttta ttagaaaaat
    24421 catctatctc acatattttg attatcaaag tattttctgt gaacagaact atagaattcc
    24481 ctaaaacttg tccattattt caaaatttgt tatagtcttt ttattcaaag cgtaaaagga
    24541 gatcctatgt taaaagaaaa aatgaaaact ctccccatgc tgccgggtgt ctatcttatg
    24601 aaagaccgag atggtaaggt catctatgtt ggtaaagcaa aacggctcaa aaatagagta
    24661 agcagctatt ttcatcagaa taaacagcat tcaaaaaaag tattacgtat gattcatcat
    24721 ataacagatt ttgatttcgt cgtagtagac acggaattag atgctttatt gctcgagtgt
    24781 caattgatcc aacattaccg tcctttttat aaccgacaaa tgaattattt cagtaattat
    24841 aattatgtac atattaccaa taagggattt gtattaacag atactcctac ggctcgaaca
    24901 tacgggcctt ttcgtctcta taaaaaaatg ccgtctattt tgcgtataat ggaagaaact
    24961 tatcaaatgc cttggttgtc tgaaattagt ttgttagctt tgcgagtcca gcttcctgac
    25021 ctacaggaaa tgacttttga acagaaaaaa aaggaactcc aagggctgtt tcaaggccga
    25081 aataaaaagc tactgactta cttgaaaaaa agacaacaac atttcatcta tcagttgaat
    25141 ttcgaaaaag ccggtatgct tcaaaaagat atcgaattag ttacttattt tattagacgg
    25201 atccaggaac aaaagcagtt tttaagaaca ccatcgctca ccttcagcat gccattagca
    25261 gcagatgaga gtcagaaaaa gcactacctc gtttgttatg gtcaacttgc agaaacaatg
    25321 attacctctg gtgacgtttc tcctgacttc tattatgaaa agaaagaagc tcatttatct
    25381 ttgaaacgtc aattgtcaaa agaagaaatc gatcctgtac aaatcttgat cagttaccgc
    25441 aaaaaattag aaaaagaaca gtttgaaatt gaactgttaa acaaaaaaga agctgaaaag
    25501 caactaaatt agttgcttct cggcttcttt taggcatctt caacttcaaa tgtcaattat
    25561 cctaaaattt attggattaa gtcatagatt tccatcgcta cgatatcgat attatcaaat
    25621 tcgtattgct gatgagtatc tgtttcagtt aattcaaatg tatcggtcgc tttatcataa
    25681 gttactgtac acttctctac gccttctttt tcaaaacggc gaacttgcag ttcattatct
    25741 gctgactcga ccatagcctc cagtcgtttt atgattgcga ctaattgtga atgtgtcatc
    25801 ggaggtcctc cttttcttat ttctgttata ttgtatcaaa agcatagcct acttgcattg
    25861 attttctcta aaaaaactcg ttttttatga ttttttttct ggtatttttc cttcaggcca
    25921 ccaaagtttg atcaaagctt gggttccgtc attctcaaaa cctttatctg ccaactcttc
    25981 atatagtttc aatgcttggg tagttgctgg caatacaaga tccatttttt ctgcttcttc
    26041 taatgcaatc tttaaatctt tgataaaatg tttgacaaaa aaaccaggtg tgtaatcttc
    26101 ttttaaaata cgaggagaat aattgcttaa agaccagtta gctgcacttc cacctgaaag
    26161 cgtctcaatc actttcttta aatcaagtcc gtttttttgt gcatagacaa gcatttccgt
    26221 cattcctgtc atcgtacctg caatcatgat ctgattagcc atttttgtat gctgaccttt
    26281 cccagcagaa ccatgcaatg taaatgtcgt accaaattct ttgaataaag gcagtacttg
    26341 atcataaacg gcttcttctc caccgaccat gatcgtcagt gtaccattct ttgctcctaa
    26401 gtcaccaccg gataccggtg catctaacgc atctgcgcct ttttcttttg ctgtttttgc
    26461 aattttttct gccaggcttg gtgtacttgt agtcaaatca actaaaatat gtcctgagat
    26521 atctgcagaa aaaatccccg actgtccata gtagatttct tctacatctt tggggtaacc
    26581 taccattgta aagagaatat tactttgttc tgctaccgct tttggtgtat ctgcccagat
    26641 agctccttct gttactaaag gatcagcttt tgcttttgtc cgattataga cagctacttc
    26701 gtgtcctgct tctagtagat ggcgtgcgac agcacttccc atgacgcctg ttccgataaa
    26761 accaagtttc attttttcga cctcctaaaa atcaaaaatc cttgcttcta tgctagtata
    26821 cgctagaaac aaggattttt tcataaaaaa tgtttgaatt ttatctttta aatgaattta
    26881 attttatttt gattcgttca atcgagtctt cttcttcttt tcttcgcatc tcttcttcat
    26941 gataaacaac catgtcttga taaaattcga tcatagcttc gccgaaatgt gtagatgaaa
    27001 tatcataaag tttagcttct aaaacttttg gatctttagg aacttcactg gaaatataat
    27061 caataagtgc tggtgcaaag tcttcgtcgg tttcaaaagt tttccctaat gaaggatgat
    27121 cgaacaatcg gttcaaataa gcatttcctt ccacgacaca aggtgttccg gctgccatcg
    27181 cttctgtata agtaagccct tgtgtttctg aagtagaagc actgacaaaa taatctgccg
    27241 ctttataata aagtgctaca tcattgttgt ccacttctcc tgtgaactgc acaaattctt
    27301 ctaactgcag ttctgagatc aattcctcta actcttctac ataaggtcct tttcctacga
    27361 taactaatct ggcgttagga taagcggaga tgacctgagg aaacccatta atcaaggctt
    27421 ggatattttt ctcataagaa atcctgctta aagaaagaat catcaaatgg ttttcctgaa
    27481 gacctagtga ctcccttaat ttgctgatat cctgctgagt gatatcagaa cgtttgaatt
    27541 tttcaatgtc gataccagta ggaatcacgc gaagcggtgc tattacacca tagctgcgta
    27601 atgtatcaat aacacgttca cttggacaaa ctacacctgt cgtatgattt gcaaataatc
    27661 tagacaagta ttttacatgg gattgcctga tcacttttcc tttaccgata taatgaaggt
    27721 agtcttcata catcgtatgg taagtatgga tcagaggtat tttcaacttc ttagctacca
    27781 tcttcccaag tatccctgct ccgaattctg tatgggtatg aataagatct agttctagtt
    27841 ctttggcaac caaataagca taccacatgc ctctaacaac aactcttcga tccttgaaag
    27901 aaataaatgg tacacttggc attcgaatca catctttttc caacttatcc gcattaggat
    27961 cggtcgttgt aaaaatatag acttcatgcc catgtctttc taattcttgt tttaatgttt
    28021 tgatcgatgt agcaactccg ctgacttgcg gaaagtaggt atcggtaaaa aagccgattt
    28081 tcatgttttt cgcctccaaa ctagtccttg acctgattta ctttttttaa tttgctagag
    28141 actttttcat tcagtacact ttcgtataca tttttaagtt cttcaccgat tttattgata
    28201 cttcgctctt ttgctgtttg ataaccggct tgtgtcgtac tcggtagttc ttgattggta
    28261 atctgttgga tcagtcggat aaattcttcg tttgtcttcc ccatatagca gttcttgcta
    28321 tcttctaacc agccattata cactggaata tctcgaacaa gtacgttttg tttgcttgct
    28381 aaagcttcta aaacaacgat cccttctgtc tcttcataag aagggaaaaa gaatagatct
    28441 gcacctgaat acgcaccttc tatgatctct ccacgaatgt aaccaggaaa caggacattt
    28501 tcaggatgat cttcttttac aattttccgg atgttccgag gaatcgagta catgggaaca
    28561 tgaccaaacc aaataaatgt gtattcgggc atcttcttag caatttctac gaaatcaatg
    28621 attcctttac gttcgaaaaa caaaccgaca caaatgatta ctttttgatc cggagacagt
    28681 ttgaaatatt ctctgaactt ttgttctttc attggatcag ggtgatattt ctctaagtcg
    28741 atcccattgg aaatagcttg aatcggcaca cggataccat agctttccaa caactgtttt
    28801 gaataaggtg ttggcgtgat cagatagtcc gctttttggt acaatcctac cagatatttt
    28861 tttacgatag gagaaagctg gttcgatctg ataaaggaat ttcggaaatc ttcctctgta
    28921 gaatgcgcat gatagacaac tttttttcct tctcttctgg ctttattgac catattatga
    28981 ctattgattc cgtatgtatt gatgtgcagg atgtcataat cttctttttc atctagtgta
    29041 taagagatac cgacttccgt cagagcacgt ttttgatgat caagcgcacg cccgattcca
    29101 gattttgcaa gcatcttttc actttcaaaa tataataata ccttcacctt acgtccccct
    29161 tcacttatat agtataacat agcctacaaa ctgtcactca aacttaagcc ctatttttgt
    29221 ctattcttac tgacaaaaac gttttcttaa aaaagaacaa aaaaactggc gtagctcttt
    29281 tacaagccac accagctttt gatacgttgt ttattttaca tattcatgaa caagttcaac
    29341 gacttcttcc atcatatcgc attctttcaa tgcacgatca gcaagttcag ccattttcgc
    29401 tgtgtccaag cgtttcatta aactacgcgt tttaagaata gatgtcgcac tcattgagaa
    29461 ctcatctaga cccataccta ctagcaatgg aacagctgtt tgatctccag ccatttctcc
    29521 acacatacct gcccattttc cttcagcgtg tgctgcatca attacatttt tgatcaaacg
    29581 taaaattgat gggttatatg gttggtacaa gtaagaaacg cgttcgttca tacggtctgc
    29641 tgccattgtg tattgaatca aatcgttcgt acctacagag aagaaatcta cttcttttgc
    29701 aaacttgtct gctaagactg ctgctgctgg aatctcgatc atgatgccga cttggatcgt
    29761 atcagatact tccttgcctt cacttatcaa tttttgtttt tcttcttcaa agatcgcttt
    29821 tgctgcgcgg aattctttga gtgtagctac catagggaac atgatacgta aattaccatg
    29881 aacagatgca cgaagcaatg cacgcatttg tgtacggaac atttcgtcgc cttgttcaga
    29941 taaactgata cgcaatgcac gatatcctaa gaatggattc atttcatgag gtaattgcag
    30001 atatggtagt tctttatctc caccgatgtc cattgtacga acaactacag gttttccttc
    30061 cataccttct aatacagctt tgtaagcttc atattggtca tcttctgttg ggaagtctgg
    30121 agaatccata tacaggaatt cagtacggta cagaccaact gcttctccac cattattatg
    30181 gacaccaact agatcttttg gtgttccgat gtttgcagct aattcaaaat gcttgccgtc
    30241 agctgtgaca gtttctgcat tttttaattt ttcccattct gctttttgtg ctgcgtaatc
    30301 tgcacctgct ttttcaaatt cagctttttg ttcatctgta ggatcgatga ttacatcgcc
    30361 ttcgatcccg ttgacagcca aaatatcgcc ttctttgact ttagcagtaa tttcttttgt
    30421 cccaacgatt gctggaattt ctaaagaacg tgccatgata gcagaatgtg aagtacgtcc
    30481 accaatgtct gttacaaacg cttttacaaa attgcgatct aattgtgctg tatcacttgg
    30541 agtcaagtca tgtgcaacaa cgaccacttc ttcgttgatc attgatggat ttggtaaagt
    30601 aacgcctaat aagtgcgcta aaatacgttt agcaacgtca cgaatatctg cagcacgctc
    30661 ttgcatataa gcattatctt ccattgcttc gaacataccg atatacatat ccgttacttc
    30721 ttttagcgct gattcagcat ttacgctgtt gtctttaata ttttgcttga tttgacctac
    30781 catttctgga tctgaaagaa ccatcaaatg ggcatcaaat acttgcgctt ctgcttcacc
    30841 taagctttgc gctgcttttt cacgaatttg ctgcaattct tcagtagatt tagctaatgc
    30901 agcatccaaa cgagcttctt cagccgcagt atcttctact gttgccttac tgaatgttaa
    30961 atccggttga actagcaggt aagcttttgc aacggctaca ccgtcactag cggcaatccc
    31021 ttttagcatt tcaaccatta ttcagataag ccttctttct tcattgtttc aacgattgct
    31081 gccattgcat cagcttcgtc agcaccttct gcagtaatag taacatcaga accttgacct
    31141 acacctaaag acataacgcc catgattgat ttcaagttta ctgatttacc tttatactct
    31201 aagtttacat cagagttaaa tttgcttgct gtttgtacta atagtgtagc tggacgagcg
    31261 tggatcccag tttctgctac tacgtgaaat tctttctttt ccatattaat cggtctcctt
    31321 taagtgagtg ttgtttttat gtgagggttt tcttttt
//
LOCUS       DANKKG010000019        30236 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000019 DANKKG010000000
VERSION     DANKKG010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30236
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.019"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            665..1534
                     /locus_tag="PW004_000740"
     CDS             665..1534
                     /locus_tag="PW004_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HDL2725733.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1743..2768
                     /locus_tag="PW004_000741"
     CDS             1743..2768
                     /locus_tag="PW004_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HDL2725734.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2807..3757
                     /gene="manA"
                     /locus_tag="PW004_000742"
     CDS             2807..3757
                     /gene="manA"
                     /locus_tag="PW004_000742"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HDL2725735.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            3851..5554
                     /locus_tag="PW004_000743"
     CDS             3851..5554
                     /locus_tag="PW004_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2725736.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5556..7589
                     /locus_tag="PW004_000744"
     CDS             5556..7589
                     /locus_tag="PW004_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDL2725737.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7600..8040
                     /locus_tag="PW004_000745"
     CDS             7600..8040
                     /locus_tag="PW004_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2725738.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(8240..9367)
                     /locus_tag="PW004_000746"
     CDS             complement(8240..9367)
                     /locus_tag="PW004_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2725739.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLLDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(9370..10047)
                     /locus_tag="PW004_000747"
     CDS             complement(9370..10047)
                     /locus_tag="PW004_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2725740.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            10311..12077
                     /locus_tag="PW004_000748"
     CDS             10311..12077
                     /locus_tag="PW004_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HDL2725741.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            12092..12529
                     /locus_tag="PW004_000749"
     CDS             12092..12529
                     /locus_tag="PW004_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2725742.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12542..13693
                     /locus_tag="PW004_000750"
     CDS             12542..13693
                     /locus_tag="PW004_000750"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDL2725743.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(13755..14453)
                     /locus_tag="PW004_000751"
     CDS             complement(13755..14453)
                     /locus_tag="PW004_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HDL2725744.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(14446..15798)
                     /locus_tag="PW004_000752"
     CDS             complement(14446..15798)
                     /locus_tag="PW004_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HDL2725745.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(16021..17421)
                     /locus_tag="PW004_000753"
     CDS             complement(16021..17421)
                     /locus_tag="PW004_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HDL2725746.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            17582..18319
                     /locus_tag="PW004_000754"
     CDS             17582..18319
                     /locus_tag="PW004_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HDL2725747.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            18444..18923
                     /locus_tag="PW004_000755"
     CDS             18444..18923
                     /locus_tag="PW004_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HDL2725748.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            18920..19687
                     /locus_tag="PW004_000756"
     CDS             18920..19687
                     /locus_tag="PW004_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725749.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            19917..20486
                     /gene="lepB"
                     /locus_tag="PW004_000757"
     CDS             19917..20486
                     /gene="lepB"
                     /locus_tag="PW004_000757"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDL2725750.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(20590..21192)
                     /locus_tag="PW004_000758"
     CDS             complement(20590..21192)
                     /locus_tag="PW004_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDL2725751.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            21298..21767
                     /locus_tag="PW004_000759"
                     /pseudo
     CDS             21298..21767
                     /locus_tag="PW004_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            21873..22457
                     /locus_tag="PW004_000760"
     CDS             21873..22457
                     /locus_tag="PW004_000760"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HDL2725752.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            22460..23533
                     /gene="prfA"
                     /locus_tag="PW004_000761"
     CDS             22460..23533
                     /gene="prfA"
                     /locus_tag="PW004_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HDL2725753.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            23526..24365
                     /gene="prmC"
                     /locus_tag="PW004_000762"
     CDS             23526..24365
                     /gene="prmC"
                     /locus_tag="PW004_000762"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDL2725754.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            24358..24537
                     /locus_tag="PW004_000763"
     CDS             24358..24537
                     /locus_tag="PW004_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725755.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            24559..25584
                     /locus_tag="PW004_000764"
     CDS             24559..25584
                     /locus_tag="PW004_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HDL2725756.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            25775..27019
                     /locus_tag="PW004_000765"
     CDS             25775..27019
                     /locus_tag="PW004_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDL2725757.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            27172..28119
                     /locus_tag="PW004_000766"
     CDS             27172..28119
                     /locus_tag="PW004_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL2725758.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            28253..28882
                     /gene="upp"
                     /locus_tag="PW004_000767"
     CDS             28253..28882
                     /gene="upp"
                     /locus_tag="PW004_000767"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HDL2725759.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(29021..29263)
                     /locus_tag="PW004_000768"
     CDS             complement(29021..29263)
                     /locus_tag="PW004_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725760.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            29384..29456
                     /locus_tag="PW004_000769"
     tRNA            29384..29456
                     /locus_tag="PW004_000769"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29417..29419,aa:Thr,seq:cgt)
     gene            complement(29575..30213)
                     /locus_tag="PW004_000770"
     CDS             complement(29575..30213)
                     /locus_tag="PW004_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDL2725761.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT        10267 a         5362 c         6136 g         8471 t
ORIGIN      
        1 aaagtcgtgt cacagtccct ttttccgaat aaacggtgtt caatcagctg tccttcggta
       61 ttagctcaaa aaacggcaaa atatgagaag ctatttttcc gttttgtatc tctaatactc
      121 aggtcaaacc gctgatttta caacctcttc cttaaacggt gttctcctgc caactcctcg
      181 gtcacaagtc acaatagtca acaaacctga gaagctgttt tttgaaattg ctccttgtga
      241 ctcggagtta aacggcaggc tcacaacctc ttggtaaacg gtgttcaaaa gccagtttct
      301 cggtattaag ccaaatttat gcaaaaatgt agaaagccat tttcacaaaa tttttcttac
      361 tactcgaaac taagcgactt tttcacaacc tcttccttaa acggtgttca aaagcttgca
      421 tctgcggtaa taagcccaac caaacgaaaa ataggaggag ctatttttgt ttggctgttc
      481 ttattacttg atgcagaaca gctttttcac aacctctttt tataagagtt tttactcatt
      541 aatttacaaa aaacaagcaa aatgcgaatt ttctcacatt ttttttaaca aaatcaaagt
      601 tattgcttga aagaagaaga atccatcgtt aaaataaagc ttgacgtaga ggggagcaag
      661 aaatatgaaa gattttcaat tgggggctgc cacctattta aaaagtgtag ccattcgggt
      721 gaaagaccga gataaaatga ttgattttta caagaacgtt gttggctttg atttgaaaag
      781 agaagaaaac gaattagcca ttttaggaac agaagaagct ggaagtgaaa tgttgctgct
      841 ggaagaaagt cctagagcga atgatcacat gggggtgatc aaaaagctga accgtttttc
      901 attgattatc cctagcacag aagaaatggc agatatcctt tgtcgtatcc gtaaaatgaa
      961 ttatccaatc aaagaagctt tagaagatca tggacgtatg gggattttgc tcgtagaccc
     1021 agaagagaat gaattggaaa tttaccatga agagaataag aacgaccaaa gagaaattcc
     1081 agaaacaatg gatcaagaag cattattaag aaaatctgaa gaaaaattca gtaaactatc
     1141 aacaggttct tattttgaaa aaatccattt aaatgttact aacttaacaa aagaacgcca
     1201 atttcttaat catgtacttg gtttgaaggt acataatgaa gaagagggca tccatgtatt
     1261 gaatgaagga gatttccatg ttggtttgac agaagctagc ggaggcgcaa ttgatttgcc
     1321 tacagatgaa gtgattggac ttgattttct gaaattcatg atcgatactg aaagcatgga
     1381 aggactgatt gctcacttgc agcaactatc agttgatttc ttcttagaca aaaagcacac
     1441 agtactcaca atctatgatg ctataggaat cgaatggtgg ttcgtcagtg agccaaaaga
     1501 aaagaataaa actgacattc gatgcgacgc ttaacttttt ctttcttaac gattgtcgca
     1561 tgtctcgtca ttttatgaac ggtcatacta tcgcttattc tagagataga aagagaaaat
     1621 agtataccgg gggaaactgc gcggtttttc gcgcagtttt tttgtgtaaa aaaggttgat
     1681 tcctcataga ttaagcttta agcttggact gtaagaatct gatagaaaag caggagaaaa
     1741 agatgtatcc acacacgaaa cagcttattt tgaaaaagat ggaagaagga ttttatccag
     1801 gtgtagcagt cgccttcata aaagaaaatc aagtagaaac atttacagcc ggctttgccc
     1861 aagtcgttcc tgaaaagcag ctgatgacag aagatctgtt gtttgatgca gcctcactga
     1921 cgaaagtcgt tgcaaccact tcattagttt tacgtctatt agaagatgga aagatagaac
     1981 tcgatcaacc gcttcataaa tatcttcctg aatttgaaaa tcagacaatc actttgcgtc
     2041 atttactgac acatacttct gatatcaaaa catggatacc aaacagagat aagctatctc
     2101 aggaagaatt gatcgccgct tatttgaagc tagaaccagg tgagcaacta ggaaaagaag
     2161 taaaatatac agatgccggt acgattcttc ttgggttgat gctagaggaa atttaccaag
     2221 aaactgcagt ggaaatcttt caagaacaag tattggaacc attaggaatg atgaacagct
     2281 tatttcttcc ttttccttcc aaaaaaatcg ttcccacaga acaactgcct tcgggtgagg
     2341 ttttgcgtgg acaaactcat gatccgaaag caagagtttt ggctgaacat gcaggtaacg
     2401 cgggtctctt tattgatttg aaagacagca ttacttttgt ccaaatgtat ttgaacaagg
     2461 gaaaaatccg taatggtcgt tttttagaag aagagacgat tgattcttta ctgcaagatc
     2521 aaacaccgaa aaaaaatggc tatcgttctt taggatggga cttaaaaaag gatgtagaag
     2581 acggtcatgc gattcttttt catactggtt atacaggtac gtttttcttg attgatataa
     2641 aaacacaaga agcgttgata tttttgtcaa atcgcgtaca tcctgtcgat catcgtgcgg
     2701 agtatctcat atgtcgagat gaaattgttg aaaacttttt gaaagagaaa gcggtattac
     2761 cgaaataatg ctataatagc tatgaaaaat gagaggagtt tgaattatgc aggaacctat
     2821 gtttttaaaa ccagtttttc aagaaaaaat ttggggagga agtcgtttac gttcagtctt
     2881 tggttttgat atccctaacg ataaaatcgg agaagattgg gcgatcagtg cgcatcctca
     2941 tggtgtcagt gtagtagaaa atggcgaatt caaagggaaa agattagatg aactatggtc
     3001 agaacacaag gaattgtttg gccatccttc agaaccagtg tttccattat tgatcaagat
     3061 cttagacgca gaagatgatc tatctgtaca agtacatcca gatgatgcct atgggatgaa
     3121 acatgaaggt gaattaggaa aaactgaatg ctggtatatc atcgatgctg aacctggtgc
     3181 agaaatcatc tatggtcatc atgcaaagac aagagaagaa ctagctgaga tgatcaaaga
     3241 tgggcgctgg gatgaccttt tgaagaaagt accagttaaa aaaggtgatt tcttttatgt
     3301 accaagcgga acgatccatg ctattggtaa aggaatcatg attttagaaa cacagcaaag
     3361 ttctgacacg acttaccgcg tgtatgacta cgacagaaaa gatgcgcaag gacaaacaag
     3421 agagctgcat atccaacaat cgatcgatgt aacaactgtc cctgcgaaaa caccagaatt
     3481 acaaatcaaa gaagtccgaa aagggaattc atcgatcgtc acttatttag aaactgagtt
     3541 tttcaatgta tatgagtggg atattaaagg aatcacatct ttcaaaaaac aagcccctta
     3601 tacattgatg acggtaatcg aaggagcagg agaccttgtc gttgacggaa aaacatatcc
     3661 attagaaaaa ggaacaagct gtattattcc aaatggcgtt tctgagtggg cggtccaagg
     3721 tgaactagca atcattgcgt ctgaaccagg caaatagttc ccagatagct gaaagcagca
     3781 gacggttttt cgtttgctgc tttttctaca taaaatccgt ccatcaataa atagaagtct
     3841 ggtgaaaaag atggaataca aatggaaagc aaacaacagc agtcgcgtgt ctatgaaaca
     3901 actgagaaaa gctggacatc acttaaatga aatgatcatg ccaaatctaa gtctgtttat
     3961 cgcttgggga atcttgtcat tagcaacaaa aggtgtgtct ggcgatttgc acaccacatt
     4021 agaagaggta tgccattgga tgatccagct gcttttaccc atcttgatca gttattcagg
     4081 cgggaaatcg gtagggggac aaaaaggggc agtcgccggt gcaattgctt ctttagggtt
     4141 gatcgctcgt tccaatgctc cacaaatcgt aggagcgatg attatcgggc cgcttgccgg
     4201 catcgtatat gaacaatttg tttctagatt ccatgtgaaa ttcaaggctg gatatgaaat
     4261 gcttgtcagc aacctactag taggactgac tggtagcgtt atttgtataa ttggaatcgt
     4321 ttttattgaa ccagttttaa atagtgttca cttgttgctc gcctccgttg tgtcttggct
     4381 agtcacaatg aatctgttac cacttgtcag tctgatctta gaacctctaa aagtactatt
     4441 tttcaataat gcaatcaatc atggtgtatt gacaccttta ggaatcgaat tctcacaaac
     4501 agtcgggtac tcttccttat ttttgatgga ggcaaatcct ggacctggtt tagggatctt
     4561 actttctatc ctttgttttg cagaaaaaca ggaaaaagta aatgcgagtg gagcattgat
     4621 gatccatgcg attggcggga ttcacgaaat ctattttcca tttgtcttgc tgcgaccgta
     4681 tctattttta gctgtaatgg ctggtggggc aagcggcacg ttgatatttc aatggttaga
     4741 tacgggatta gctgcacctg tatcgcctgg gtcactactc gtgatttttg ccaatgcgcc
     4801 ttatccacaa ctagctgggg taagtatagg gatagcaatc agtacatttg tgaccttcat
     4861 ttgtgcagcg atactattga aattaccaaa taaagaaaaa gagaacaaaa cggtcaaaga
     4921 aaggggagaa atcttggaac catctatcat tcattccgtc atttttgctt gcgactcagg
     4981 tatgggatca agtgctatgg gagccgccct gctgaaaaag aaattagcag cagaaaaaat
     5041 cgatctttct gtcggataca gctctatcta tgaattgaat aaccaagtaa atcagctggt
     5101 tatcgtgcaa catgaactag cgggtcttgc aagagaaaaa gtacctcaaa gtcaaatcta
     5161 tgtactggat cactttttgg aaacagatat cttgctgtca cgaataaaag aagcgatcca
     5221 atttccaaag cgggaagaaa aaaaagagat agcagatatc acagaacaaa aaacgtcgcc
     5281 tgctaatatt gtatttttgt ataaaaacaa cagacgcggc tcacaaacta tgggcatgac
     5341 gattttaagt caattagcaa aaaaagcgaa aaaacagttt gttatctcaa aagaatcaat
     5401 agaaaaaatc aaaatccaag aagaaacgat ttatatcgca caaagagaac tagctgtaac
     5461 tgaacagcta gaagaaaaga tccctcggct agttctagtt gaacattggg tagctacgaa
     5521 tgaatacgaa aaactcgtga agggagaaat ataagatgta tttatcgccc agagaaaaac
     5581 aattattagc agaatttctc agtagcccgt cacctgtatc tatccaaaaa atgatgaatt
     5641 tattgaaggt cagtaaacgg actatttacc gcgaattaga taatcttgaa gtctcattga
     5701 aatcggtaaa tgcttctttg aaaaaagcag ctagaggctc atttgtcatt gaggcatcag
     5761 aagaagtatt agagcaatta aaagaaaata cagatgggaa aagtaatgac ttatccactg
     5821 ctaaacgaca acacgcgatt ttagccgagt tgttactgac gacagagccg ttgagtcttg
     5881 ctcatttcat ggaatcatat cgcatcagta ataccacttt ttatgcagat atcaaacagt
     5941 tggaagaaag tattcgccaa ctgcctttgg aaatcattcg gaaccaggga tacgagatcg
     6001 aaggaccgga aaaataccgc cggcttctga cagcgaatgt attagaatta gaaatcaatg
     6061 aatatgaact ttttcatagt atttctttcg atgcatcact gaattatttt tttcgattcg
     6121 ttgatccgca acatctatct cttgcacgca aagtaatcgg ggaagaattg atccaaaaaa
     6181 agaccaatct aagtgataga aagcttgaac atttggtttt gatgctgaca ttgacgatgg
     6241 agcgtgtgac gaaaaatcat ttactgacaa atgagacata taatagtctg gcaaacaaag
     6301 aattactaca gacagctaaa cgattgtttt cacggatcgc ttctgagaca aaacaacttt
     6361 atccagtaaa tgagatcgtc ttttttgcag gattattaaa tgacttcacg aactcatttg
     6421 atcagaattt ctttgaagag acgttcgata cacagcttgc ttatttagta aaacaactga
     6481 ttcaagaagt aagtgaagag acgaatgtcc atttttatga agatgaaact ttgtacaaaa
     6541 tgctgttgac tcatttatct ggtgtgtttt caagagccgt tttacaagaa gagacactga
     6601 ccaatccgat tttagaaaaa atcatggttc aatacgagga agttgcgaat gcaatccgta
     6661 aagcagtaga gcggattttt caagaaaaaa aattatccga agaagagatc gcttatatgg
     6721 tccttcattt tgcgaattca ttggaacgaa gtccgaaaga aagcaatatc agtattgcag
     6781 gattttcgcc aagtggatta gcttctacta gcatgttgga actacgattg aaaaggtatt
     6841 ttcccttcat ccaacaaatt catttctatc ggattgctga tttaaaaaaa atcgatttac
     6901 aagaagaata tgatgttgtt atttctacat ctattttacc aggatacggt ggaaaatata
     6961 ttttagtctc cccgctactg ctagaagatg aagtcaagca gctaaaagaa gctttttcgc
     7021 atattgagaa gaaaaaaagg caaaaaatta cgtataacag caataacgag tggatgatag
     7081 aagatactta tgaggaaaca atgcatttca tggatcaaat caatcaatta ctcggtcgtt
     7141 tttttatcca agaactggat aatcctgaga cgatttccga aacgattcgc agcttatttc
     7201 cttattttcc tgaaggacta gtaacggatg aagaggtggt tcatcgccgg ctgcttaagc
     7261 gatatgaaca agcacctatc ggtattcctc atacaaatat ggggttattc catacatcca
     7321 gctcattgat ttctaaaccg atcttttgta ttttcaattt gaaacatccg ttgaccattg
     7381 aagggatgga tagacagcca gtcgatttgt cccgtatgtt agttatgctg gcgccttcgc
     7441 caatccaaga aaaagacggg attcttttag gaaagatcag tggagcaatc atcatgaatg
     7501 acttgaacac cgagatcttc cgctcaggaa ataaagaaat cgtttatcag ttgttagcga
     7561 aaatcctgat tgaagaaata aagaaatgaa ggtgaaaaga tgatacagct accagcagaa
     7621 aaaatattgc tcgatcaatc ctatcaaaat tggaaagaag cgttgaccga gatcggccag
     7681 tggatgaccc aacaaggggg aatcaaaccg gcatatatcc aatcaatgat cgaaagacaa
     7741 gaaaaagcga gtgtctatat cgggaatttc gtagccttgc cacatgggac tgacaaagat
     7801 gaagaagtag tagaagaagg aatctattta gtccaagtgc ctgatggtgt ggactttgga
     7861 acaaaagagg aaccaaaaat cgcgacactc ttatttgtag tggctttaaa aaaagaacat
     7921 cagttagcgg tgctccaaga tttagccttt ttctgttcag acatcgacca agtaatggcg
     7981 ctcagtgatg cggaaaccat tgaagaagtc caaatgattt ttgaacatgg acatatataa
     8041 ccaatgttca cagtaaaaaa agctgccttg catcaagtaa taacttcaac caagataaaa
     8101 ataagaggag ctatttttgt tgggaggttc ttattactgc ggatgcaagc ttttgaaaac
     8161 tatttattct agaaaaccgt ttatttgaaa taggggacgg tgacaaggtt tttgtcaccg
     8221 cccctattat ttttgtgttt taatagctct tttttatata gatagtgccc atacctatga
     8281 ataataaaac gatactccat ataccaagtg tcaagatacc attcatgaaa gtaacctgtt
     8341 ctgaagctag tggcgagaaa atatctcctc ctatgatcac attttgaaaa tcaacataag
     8401 aagttggcaa gtaatgactg attgattcta tccatccaat cgaagaaaac aagcgatact
     8461 ctgctaagaa aagacaaaga agtgtaccta aaagattgac tagagaatgt tcaaataaag
     8521 cagatagaag aaaactgatt gtacttaaaa agagcaccca caacaacagg aacagaaagc
     8581 tctggaaaag atactcatac atcggcagga ttcgtatctc ctgaccaatg atcgttccta
     8641 caggataacg gaagtctccc aaaccatttt tgattattac tagtatagag acgatcaaaa
     8701 atggcatcac gagacttagc atagctgcac cataataaat ggtaaaacgg atgctatgtt
     8761 tctttaccat actatccagt aatagattga ctagagagat tacctgcttt cttttttcat
     8821 aagagatgat ggaagaaaga aacaaggcgg ttatacataa aatcaaaaaa ctagaaatca
     8881 ttcctgaaaa aatattttcg tagtaatttg ctaaagagag atgcgctggc aagtcaaaaa
     8941 tagaatatct ttggatctga tgttctttca tataacggag caactcgaca cgtttttgtt
     9001 gttcaatgat tggaattcct tgtctttgac cagaaatcag tcgatccagt atatcttttt
     9061 cataatgata agttgcttcg accatttgct ggacattccc tgtgtttttt gcatgtaaga
     9121 ttgcttcgat cagtttgttg cttttctcta aactttccac aaattctttc tcattctcat
     9181 cttctttcaa cgtttcaatc aaacgttgca tctcttcata gttatgattc agttcatcga
     9241 attctgtata agcaagttta tgatcaaaaa aaagtgggaa tgcaaagagc acgaccaaca
     9301 atagaggaaa taaaaagttt actttgtttt ttagtaagag attgcgttca aatgtaagta
     9361 aagacatagt tacacctcat ataatttacg ataaatagct aaaatctctt ccatatttga
     9421 ggattctgtg acgatttctc cattttttag aaaacatgct ttagaacaaa tcgattggat
     9481 attcgcgata gcgtgggaag aaagaagaat cgtcttcccc tctttctgcc attgtctgat
     9541 gaggctattc aagatatcaa tacttcctgg gtctaatccg ttcaacggtt catcgaataa
     9601 catgacagat gaatcactga tcaagtaagc agcaagcaag acatgttgtt tcattcctag
     9661 agacattttt ccaatcggca aagtaaggta ctcagtcatc ttcaatcgtt ggaaaacagt
     9721 ttggcattct actttgctct gccaatagta tttgatcaaa gtcaaataat caacagccga
     9781 tagatagtcg gataactgat tcgttgattc tagaaaaaag aaagaccggc gataatctgt
     9841 ataagcagta ggagagatgt tgtgaaggga caattgatca taagttgttg gcagcagttg
     9901 ggcaatgcca ttcagcaaag ttgttttgcc aaatccgttc ggggcaatca aagcagctac
     9961 ttctcctgat tctactaaaa gattcaattg tcgaatcaca cgttttttac cacgtttcat
    10021 gttcatgttt ttgatcagta atgtcataga taggcctcca atataaaatt taataacata
    10081 atagaatgga aaacatctaa attaaatgct aacaaaagtt attgtttgca tattataaaa
    10141 aaattaaaat aacagtttta tttaaaattg aagttttctg ataccgaaat ctttttattt
    10201 tgctgacata ctcaaacctt tggcacaaag tttcgtgcca aagtacgact atttctcttt
    10261 ttagtaagcg gatacaatta gagtatcaaa aggagagagg gagagacaaa atggaacgga
    10321 cgattcccaa aaacaacatt tcgctaaaag cgagggtaca aaaattaggc agtactttat
    10381 caagcatggt catgccaaat attggagcac tgattgcctg gggtgtatta acggctctat
    10441 ttatcccaga tggttattta ccaaatgagg ctttcgctac catggtaagc ccaatgctaa
    10501 cttatctgat tcctttattg atcggttata ctggcggaaa agtaattgct ggtgaccgcg
    10561 gatcagttgt tggggcaatt gcaacgatgg gtgtgattgt cggaacagat attccaatga
    10621 tgctaggcgc gatgatcatg ggacctcttg gtggatatgc catcaagaaa tttgatcagc
    10681 tattccaaaa acggatcaaa tccggttttg aaatgctagt caataatttt tcagcaggtc
    10741 ttatcggttt tggattagcg ttattaggtt tttctgcgat tggtccagtt gtcgatgcat
    10801 tgactcaagc aatggcaaaa ggtgtagaga tcattttatc tgcccatttg attccattga
    10861 caagtatttt tattgaacca gcaaaaatct tatttttgaa taatgcaatc aatcatggga
    10921 ttttgacacc attgggaact gaacaagtag cagaggctgg aaaatctatt ctattcttgc
    10981 ttgaagcaaa tcctggtcca ggacttggcg tattacttgc ttttactttg tttggcaaag
    11041 gagcagcaaa atcttcagca ccaggagcca tgatcattca ctttttaggc gggatccatg
    11101 aaatctattt tccttacgtt atgatgaaac cgttattatt cttatcagtt ataggcggag
    11161 gaatgtcagg aagtttcatt ttccaattga tggatgctgg actaagagcg ccagcttctc
    11221 cagggtcgat cattgcgatt ctagcgatga caccgttcaa ttcttacctt cctgttattt
    11281 tgggcgtgat tgttgccgca gctgtttcgt ttggtatttc tgcagtaatt ttgaaagcgg
    11341 atgcgaaaga tacaaatgaa gacttcgaaa aatctgttca agagacaaag gcagctaagc
    11401 aagcttccaa aggtgcccaa ggtattcctc aaggagctac attctctggc gtacaaaaaa
    11461 tcatttttgc ttgtgatgca ggaatgggat caagtgcgat gggcgcttcg atcttgagaa
    11521 aaaaagtaca ggaagcagac ttgcctcaaa cggtgaccaa tcaagcaatc agccaactga
    11581 cagatgactc gaatacattg atcgttactc aggtcgaact acaagaaaga gcaaaacaaa
    11641 aagcaccgag cgcgcgtttt gtctcagtag aaaacttttt gaattcacct cgatatgatg
    11701 aaatcattca agaattaagc agcaagcaga aaacatcagt ttctgaaatt gagaatgagc
    11761 cagcagaaga gtttctttca gcaaacatca aacaaatcaa agagattcta cttgtccacg
    11821 atgatcgttc cggatcagca acaatgggga tgaccgtgct caaagatata ttagacaaga
    11881 accatttaac tatcccgtta agaaaagtca atatccaaga tttaaaagat gaacctgatt
    11941 cactgatcgt agccaaagaa agtttagcgg aaaaagtaca gatggctgca cctcaatcca
    12001 ttcatttatc agtcaacagc atggtcaatc cgcaaaaata cgaatcgatt gcacaatatt
    12061 tgaaacagac agcttaaggg aggaaaacag catgcaaagt ttgccattcg acatgatcga
    12121 gctcaatcga tcttttaaaa ccaaagaaga agcgatccgc tattgcggtc agatgcttgt
    12181 agaagcaggg tgcgttgatg aggaatatat cgatgccatg gtagatagag acaaaatgtt
    12241 atctgtttat atggggaatt ttatcgcgat tcctcatgga acagatgaag caaaaaaata
    12301 tgtgaaaaaa tccggcatct gtgtcgttca agtaccagat ggtgtagatt ttggtaccga
    12361 agaagaagaa aagattgcga ctgttttgtt cggtattgca ggagtcggag atgagcattt
    12421 gcaacttgta cagcaaatcg cactttattg cagtgatatg gacaatgttg tccagctagc
    12481 agacgcgctc agcaaagaag aagtaacgga aaatctagca attgcttaag gagagagaaa
    12541 aatgagagca gtacattttg gtgcaggaaa tatcggtcga ggatttattg gagaggtttt
    12601 ggcagctaat ggatttgaga ttactttcgt agatgtgaat cacacgatca ttgatgcttt
    12661 aaatgaaaga aaagaatacg aaatcgaatt agcagatgaa tcaaaagaaa aaatcactgt
    12721 aaaaaatgtc aaaggaatca acaatcaaac aaatccagaa aaagtaatcc aagaaatcgc
    12781 tgaagcagat ttagttacta ccgccattgg cccgaatatc ttacctttta ttgctgagtt
    12841 gattgcaaaa ggtatccaag aaagagaaaa ggaaggaagc acaacatctt tagatatcat
    12901 tgcctgtgaa aatatgattg gcggttcagc atttctagaa aaagaagtgt ataaatatct
    12961 tccggaaaca tcatttactg acaaatatat cggctttcca aatgcagcag tcgatcgaat
    13021 cgtcccatta caacaccatg aagatccttt attcgtccaa gtggaacctt tcaaagaatg
    13081 ggtcattgac gatagccaaa gaaaaaataa aaaaatccaa ttaaaagggg ttttatatgt
    13141 agatgatcta gaaccatata tcgaacgcaa actatttagt gtcaatactg gtcatgcgac
    13201 tgtcgcttat acaggtgcct tgttaggtta ccagaccatt gatgaagcaa tgcaagatgc
    13261 gttagttgtg gcacaattaa aatcagtttt gcaggaaaca ggaagtttgc tgattgccaa
    13321 atgggggttt gatgcagagc agcatcatgc gtatattgaa aaaatcattc atcgcttcca
    13381 aaacaaaaat atctctgatg cgatcacacg tgtcgctcgg acaccattac gtaagttagg
    13441 ttatcaagaa agatttacac gtccagtgag agaactacaa gaacataact tgacttgccc
    13501 acatttgacg gcaacaatgg ggattatttt taattactat gatcctgaag atgaacaaag
    13561 ccgtcaattg catgaaatga agatccatga aaacttggaa caattgattc aagaagtcac
    13621 aggaatcaat gatccgaaaa caatcgggaa tatcaagcaa aacgtgaacc gttatgccaa
    13681 gcaagtcgct taaaatagga tacgaaaaag gggaatgtga cgaaattttt gtcacattcc
    13741 cctttttcga ataactaggc tgtttgtcct tcaagatagg gcagatccaa ttctttaccg
    13801 taacgttgtt ctaaagctag gcggatcaaa cgagctgcca ggttctcatt tcttgcaagg
    13861 ataggtccat ggaaataaga accgaagaca tttcggtaaa tgacaccttc agattggtct
    13921 tcaccattat ttccttgacc ttgtaccact ttccccaaag gtcgttcacc ttcacctaag
    13981 aaagtccgac cattgtggtt ttcaaatcca taataagttt cattgaattc ttcgttatgg
    14041 ataacgatat caccgataaa ccggttattt tcctgactta acgtgtagtg atccagtgca
    14101 ctgattcctt ggatcttttc tccattagct cccatgtagt agtgacctag caattggtag
    14161 ccaccacaaa tggctaacat gacgccatca ttttcgatat agttgatcaa agaatctttc
    14221 tttttctgga tgtcttttga aatgattaat tgctcaaagt cttgaccacc gccaaagaaa
    14281 accaagtcat atttatcagc atcaaaaggt tcatgtatac taacgatctc cgtatggcaa
    14341 gaaaccccca ttttttctgc tatatatttc aacatcaaaa ggtttccgtt atcaccatat
    14401 gtgttcatca aatttccata aaggtgggcg atattgagtt cataattagc cacggttcat
    14461 tcctcctttg ataaatcctt gagcagctag ttctttacga agctgcaata cagcagtgta
    14521 agtagctaaa atatagacat gctcagttgg caattctttg attttttcga ttacttctgg
    14581 taattctttt gtttctgtca atttattttc tggaatcccg gcaactttta gacgtaaagt
    14641 catatctgta tgacggtctc ctccagaaat aacagctggg atatccatat cggcaaagtt
    14701 ttcaaaatta ccatcccaaa tccagctgac atcgattccg tctgcgtaat tggcatttaa
    14761 taaagccact aaagaaaatg gataaggagc tagaccaatc atatcgatta cttgattgat
    14821 acctacagga tttttgacaa gtactaatgt acattttttc ccattgatat tgatgacttc
    14881 ttgtcggccg aatactttct cgtcatatcc taaaccttgt ttgattttat caggatcgac
    14941 attgaagtac tctgccactg cagtagctgc taaagcattg tagacattat acataccgcc
    15001 aacttcgatt ccgtattcat gaccatcgat cacgaaatct gctgacacat tgtccatccg
    15061 taccatatca gttaatcgat aatccaattc aggacgtttg aagtcacaat ttggacagta
    15121 atatttccct aagtttgcat aagtgatcat tttataatgc aagatgtgat gacaatttgg
    15181 acataagaca ccgtctgtat tgtaatgagc catttgttca cgatcttctt catggtcaaa
    15241 gccataatat tttctaggat tgactgtttc gatagaattg aagattggtg aatcaccgtt
    15301 actgataatc gttgcatttg gtgactttgc ggcaccgtct acaatcattt tataggtcgt
    15361 atagatttct ccataccgat ccatttgatc acggaaaata tttgtaaata agaacagttc
    15421 tggtttcata tattctgtga ctttgctcaa acttgcttca tctatttcta ggacagcaaa
    15481 ctttttctgt cctttttttg gttttgcctg caagaaagtc gatacaattc cctgtaccat
    15541 attcgcacca gtagggttgg ttagaacttc atcgaattgc tggcgcaaaa tattgactgt
    15601 taaagcggta gtcaatgttt taccgtttgt tccggtgaca acgacgactt ggtagtcttt
    15661 tgccaaacta tctagtatat gtggatcgac tttcagcgct agtttacctg gtaaacttga
    15721 tccgccttta aaaaaagttt ttaatgccca ttgcgacgtt tttccaactg caatcgcaaa
    15781 atgactgcgt attcccatta tgtaacctcc aaaaacgatt tgctgacgaa taaattcaac
    15841 taatcatacc atatgttgaa aaaaaggtta aattgctttt cttattctta agaaaaggtt
    15901 acggctgaaa gtgcatgaaa tcggacaaaa tcgcatgatt taacaaaaaa acagcttgaa
    15961 acaaaatgat tgtttcaagc tgctgtgatt ttattattcc ctcttaccta tattcccatt
    16021 ttaaccgata ataattttct cgcttggata ttcatagcct agatctcgtg tttcttttgg
    16081 cacgaataac atcaatgtat atagcattcc gatccgccca ataaacatca gtgtagcaat
    16141 cgtgattttg cccacaacag acaagtctcc ggtaatccct aaagataaac cagttgttcc
    16201 aaacgcagaa gttacttcga tgatgatcga gatcagcgtt tgttcttctg tagcagataa
    16261 aaaaacgata caaaagaaac acataccaag agaaagcata aagacaacga ctgattttcg
    16321 cacatcatct tgatcgattc tccgtccaaa aatattgatg ttatcttcac ttttgaggaa
    16381 ggaatataga tacaaaccga tgattgctac agtagttgtt cgaatcccgc cgcctacaga
    16441 actaggactg catccgataa acatcagtaa agaaaaaatg atcagtgttg tgatttgaaa
    16501 atcacccaaa tcgtgaatct gcagaccagc attacgagtc gtaatcgaat aaaacatcga
    16561 attgatccat ttgacactca tattggaatc ttggaataaa tggtcctttt ccaatagata
    16621 aatcaagaca gttcccgaaa caaacaagat aacaaaagct aacacagcta atttggtaaa
    16681 caaggaaaaa cgaaacggaa gcttcgcatt ggaacgtttg tataatagcc attcccggca
    16741 ctccatcaat acaggaaaac caattcctcc gacaaaaatc aaaaacatga tcaggatcaa
    16801 gaaaaaatag tcatgtgcaa agggcttgat ggaatcccca gtgacgtcaa acccagagtt
    16861 cgtgacagcc gatatcgcct gataaaaacc gtaaaaaacg gcatcacgcc aacgatcaaa
    16921 atagcctctg taataaaaat aaatagaaaa gaatgtccca aaaagtaatt gaaaccagat
    16981 taaaatagca aaagtgatcc gaatcagtcg gacgatacca gacaaacgcg gttgattcat
    17041 atccgtcatg atcagctgtc gctgtttcag tgtgatccgt cgtttagaaa aaatgacaaa
    17101 agcagtagaa atcatcatga tccccaaacc accaacttga aataatattt ccagcagtat
    17161 aataccgtta tcattgaaca cagaattgat atcaaaagtc gatagtccag tcacactcac
    17221 tgtactgatt gccatgaaca gcatgtccac aaaagaaaca tggctatctg gttcgcgaaa
    17281 aatcggcata taaaatagaa cgagtgataa gaccgtcatc aatatataat aaaaaacgat
    17341 gatttggatc gatgagaaat gattggcagc aaacttctgt cctcgccgct gcagccttcg
    17401 aaagaataaa tcaacatgca cagtactatc ctcctgacaa tagtcgtttc taccagtata
    17461 caaggggaat taggaatact caaagaaatt atcaaacaga taacaataca gctttcgatt
    17521 aaaatagaaa tggcttataa taaggaaaat cctagaaaaa agaaggagga ggaataacaa
    17581 attgattaat gctcaacaaa tcgtagaaaa aatgaaaaat caaaaaataa attatgatac
    17641 agttctgaaa aaaatgatca tgcaatggga gaaagaagaa gtaaagccta agatattaat
    17701 acatacatgc tgtgctcctt gcagtacgca cacattggaa ttcatgtctc aatatgcaga
    17761 gatcacgtta tttttttcaa attcgaatat tcatcctaaa agtgagtatt tgcgaagatt
    17821 acatgaacaa aaacgatttg tagaccaatt taatgagaaa actgaccatc atgttgcgct
    17881 aatcgttgat gaataccgtc caaatgaatt caccaaaatg gttttaacaa accatcttga
    17941 aaacgaaaaa gaaggtggtg ctcgctgcag tgcatgcttc aatatgcgat tagatcttgt
    18001 tgcgcaaaaa gcgcaagaac taggatacga ttactttgga agtgccctga ccatctcgcc
    18061 aaagaaaaat gcagctttga tcaatcagat cggaatggat atccaaaaga tttacgggac
    18121 acaatacttg ccaagtgatt tcaagaaaaa caaaggctat gaacgctctt tgcaaatgtg
    18181 caaagagtat gatatttatc gccaatgtta ttgtggttgc gtgttcgctg ccaagcagca
    18241 aggatgcgat ctgaaagaaa tcaatcggga agcaaaagct tacttaaaaa catcaaccgt
    18301 agtttttgag gaaaattaaa gaaatctttc ggaaaaacgt aagaaaaaaa taaagaattt
    18361 atggtagaaa taaggtagac tgtataattt gtccgtagta aagtaaggaa acaagaataa
    18421 atttcaatga aggaagagat aagatgcgaa aagtagcacc tattttgaag ccaatcgctg
    18481 tattaacaat ctcgacgatt gtcgcgacta ctacaggtat tgtgaatagt tccagctacc
    18541 gtgcaccaag ttcaagttct agtactgtag aagaaacaac accaactact tcagtctcga
    18601 catctgaacc aacaacaacg gaaagtttga atgaaaccac tacgacaagt acatgggacg
    18661 atacaacaac tagttcgtcg tatgatgaga catatacaac tgacagctca agcgaaacaa
    18721 gtaatacggt accagatggc actccatcgg attcaaccaa tccaagtaca agtccggata
    18781 caagtgctag ctccgaaacg ataccaagca gtacgactgt agatgataac acgcaaaatg
    18841 atgatgcagc acgtacagca cagcaaaacc aagattcagg aaatacccaa acgtataacc
    18901 agcaagcagg tgattctcaa tgacattttt actagacctt atcaacgggg tgcataaatt
    18961 ccttggctat ttagacatca gtcctaaata tcttaaccgt ggatatacga ttttaagtgt
    19021 gattcccacg ctttatttat tgagaattgt ttatggactt tggcaaaacc aaaactatct
    19081 tcaatttttc ttatatggac ttgcatttct agtgcttgtt tattttactg ttttaaatgt
    19141 attttattac tttttagata aaaacacgaa agccgatgtc actcagcttt ttgtgaagta
    19201 tttacctgat gaagcgttca acatccagac agaagaatcg atagataatg gaaattcgat
    19261 cgataaagtg aatacacaag aggtcatggt ttcttatgac gaagattatc aattgaaatt
    19321 agccgagaat atgcggtatt tgattggaaa tggtgagatc aaaacaaatg aactcggtcg
    19381 catggatgga tttctggtcg atagaaatac attgtatcct tattactatg tcaagaaaac
    19441 aggagagaaa gagtatgttc taaatattgg acgaagctac tcggacatgg aaaaagtagg
    19501 gaccattcga cttgactcaa cagaagacac attggaacct gtcggattgt tcatcgtcgg
    19561 cggtgatttt gtcaaagacg ggttacgcta tcatgagcct tatcgtctga aattgattgc
    19621 aaaaaaggca cagtcgcagg aagaaccagt ggtttatagt cgtagccaaa gaagaagaca
    19681 ttcataatgt gatgaaacgc cgtgtatcag tggttgcgat aaaaacgttt attcggaata
    19741 agggggatgt gacacgtctt ttgtcacgtt ccttttttat ataacgagaa agataagacg
    19801 aacacatcac ttattctttc ctttcattta taaacattgt agaataaaga aagtacaacg
    19861 aagtattgtg aaaaaacgta ataaataacg aggaaaatac aagtaggagg taagctgtga
    19921 aagaattttt gaaaaattgg gggattttga tccttgtatt agcggctatt ttattagcta
    19981 gagtgtacgt gtttacacca gttactgtaa acgggcattc gatggaccct acattaagtg
    20041 atggacaacg attgatttca tctaagatct caaactatga acgaatggat attattacta
    20101 caaaggaacc gggggatgaa gaacggatga ttgttaaacg gatcatcggt atgcctggag
    20161 atacagtcaa gatggaaaat gaccagttga ccattaatgg caaaaagtat gatgaacctt
    20221 atctggatga gttcaaaaaa gaattttcag aagataagtt acaaggagaa tatgcttata
    20281 gctcaggatt ccaagcacaa gcagaaagtt catccacctt tacgagtgac tttgaatata
    20341 ctgtgccaaa aggaaagtat cttgttctag gcgataaccg cttgatttca aaagacagcc
    20401 gaatgtttgg gttagtcgac aaagatatga tccaaggaaa agttgttttc cgttattggc
    20461 ctctttcaga aattaaaatc atgtgatgaa aaaataaaaa gaagctgtga catgtttttt
    20521 gtcacagctt ctttttccga aaattccttc ttatttcttg gagttgctca ctttttttgc
    20581 aatctcattt taagcttttt ggctgatgga taagaaaagc gaagcgtttt tgttcataaa
    20641 tagcttttcc ccacgatgga aggtatgact agtagcagag acaaagcccg ttttttccaa
    20701 tcgattattc aattcttctt ggctaaagcc attatggact ttcggatgac taatttgttc
    20761 attcttgtca aagtctacga tgatcaattg accgccagga gctaaaattt cataaagttt
    20821 tgtaagaata ttctccgtat caggaatgtg taaaagtaca agtgatagaa ggatgagatt
    20881 agcttggaca gcaggaaatt cgacagaagc atcagcgtgg atcgttcgga catttttcag
    20941 atctgctgct tggatctttt cttctgccat tttcagcata gtttctgaag catcggcaat
    21001 gatcagttct ttgaatcttt ccgcaagcgg taaactaaca agcccagttc cgcctccgta
    21061 atccagcagg actttttggt ggctgtcgcg cggtagataa gtagttagtt cttcgcgaat
    21121 aatcttggct aatgcttgac ggtcagggga atcataatgg ttagcgatgg aatcaaaaat
    21181 attttttgtc atcaaatagc tccttacgta aaactttgaa attttaactt attatagcac
    21241 gtgaaaagaa agaaagcatg ctatactcaa ttcaaaaagg gagaatctag aaagaatatg
    21301 ccgatatcgt ttgtaaaaga tagagaggaa aaaggaaagt gtgtccgaga aatattacta
    21361 gacttgcctg agtggtttgg gcttcctgaa tcaacggaaa aatatatcga ggaatcaagt
    21421 aaactacctt tatggtgtga aaaaagaaaa gaagaatatt taggctttat cactctttca
    21481 cagacaagtg aagatactgc cgaaatctat tctattgtat gggaataaaa aaagactatc
    21541 atcaccaagg attaggaaca aaacttttta aagaagcaga aggttacgca gcaaaacatt
    21601 acaaatattt gcaagtgaag actgtagacg aggggcacta ctctatatat gatcaaacta
    21661 tttgtttcta cgaatcatta ggattttcaa ggttggaagt ttttccaaat ttatgggatg
    21721 aatggaaccc atgtttagtt cttgtcaaaa aattggaaca aaagtgagca atagatttaa
    21781 tactaatctg tgcttgatga aaattctcac tgtaaaaaat gctgaaaatc aagtataata
    21841 ccataggaac aaactggaag gtgaaaaaga gcatggcaca actatttttt aagtatggcg
    21901 caatgaatag cggcaagacg atcgagatcc taaaagtcgc acataattat gaagaacaaa
    21961 ataagccagt tgtcttaatg acaagcggta ttgatacaag agatggttta ggcgttgttt
    22021 caagccgaat cggactgaaa cgtaatgcga tgccgatatt tgaagaaaca gacgtatttg
    22081 acgtaattca gaaaatggaa agcaaaccct attgtgtgct gatcgatgaa gcgcaattct
    22141 taaaaaagaa acatgtcatt gcttttacga gaattgtgga cgaattggat attccggtta
    22201 tggcatttgg tttgaaaaat gattttcgaa acgagttatt tgaaggatca aaatatttat
    22261 tgctttatgc agacaaaatc gaagaaatga aaacaatctg ttggttctgc cacaaaaaag
    22321 caatcatgaa tttacattat attgatggaa accctgttta tgaaggcgat caagtgcaaa
    22381 ttggcggaaa tgaagcttat taccccgttt gtcgaaaaca ttactttcac ccatcaatga
    22441 ttacagaagg agaataagca tgtacgatca attacaagca attgaagacc gttatgaaga
    22501 gcttggagaa cttttaagtg acccagaagt gatttctgat acaaagcgtt ttatgcagct
    22561 ttcaaaagaa gaagcaaaca cgcgcgaaac agtcgaagtt tatcgtcgtt ataaagaagt
    22621 agtacaagga atcaccgata cagaagaact gcttggtgaa aaactggatg atgaaatggc
    22681 tgaactagca aaagaagaat tatctgaatt aaaaaaagaa aaagaagtct tagaagaaaa
    22741 aatcaagatc ttgcttttac caaaagatcc aaatgacgat aaaaatatca tcatggaaat
    22801 ccgtggagca gcaggcggag acgaagcagc attattcgct ggcgatcttt tcaatatgta
    22861 ccaaaagtat gctgaagcac aaggctggaa aacagaagtg atggaagcta acgttaccgg
    22921 aatcggtgga tataaagaag tgatcatgat gatcaccgga gacaacgtat attcgaaatt
    22981 aaaatatgaa agtggtgcgc accgcgtcca gcgtgttcct tcaactgaat cgcaagggcg
    23041 tatccatact tccaccgcaa cagttgtcgt gatgccagaa gctgaagaag tagagatcga
    23101 tattgaagac aaagatatcc gtacagatat ctatcatgct tcaggtgctg gcggacagca
    23161 cgtcaataaa acagcatctg ctgtacgttt gactcactta ccaacaggga tcgttgttgc
    23221 tatgcaagat gaacgttctc aaatcaagaa ccgtgaaaaa gcaatgaaaa tcttgcgtgc
    23281 tcgtgtgtac gataagatcc agcaagaagc acaaagcgag tatgatgcca atcgaaaatc
    23341 agcagtcgga actggggatc gttcagaacg gatccggacg tataatttcc cgcaaaatcg
    23401 tgtgactgat catcgtatcg gattaactat ccaaaaatta gatcagattt tagcagggaa
    23461 attagatgag atcgttgatg cattgatcat gtatgatcaa acatcaaaac tggaagctat
    23521 gcaaaatggg taaaatgacc tatcgagaag tcctgtcacg ggcttcttct tttttagagg
    23581 atcatgggaa agaagcatat agtatccaat ttttattttt ggaacgaaaa caatggaaaa
    23641 aacttgattg gctccttcat atgaacgaag agatttcaga agaagaacag cggttgatcg
    23701 aaaccgatct gcgacttctg cttgcagatc atccacctca gtatttgcta ggatatgcag
    23761 atttttatga tcatcggtta aaggtaactg aagcaacact gatccctcga ccagaaacag
    23821 aggagttagt cgaatggtgt ttagacgaga cgcctggtgt accgttagaa gtgatagata
    23881 ttgggacagg gactggagca attgctatta gtttgaaagc agcaagaaaa aattggcata
    23941 tatctgctgt ggatctttca gaagaagcat tagaagtagc gaaagaaaat gctcagaaag
    24001 aaggaacgaa aatcagtttt tatcatggag atacattagg accagtaatg gatcagcaat
    24061 ttgatgtgat tatttctaat ccgccgtaca tcagtcggaa tgaatgggaa ctgatggatg
    24121 aaagtgtccg gtcattcgaa cctaaaatgg ccctttttgc tgagaacgat ggattagcta
    24181 tttatgaaaa aatagcagtt gaagcaccaa gcgtactaaa atcaaatgga aagatttttt
    24241 tagaaatcgg atttcgccaa ggtgaagcag taaaaaaaat atttcagcaa gcatttccag
    24301 ataaaaaagt ggcgataaaa aaggatcttt ttggcaatga acgaatgatc caagtttatg
    24361 gttgaagttg tggatgaaaa attttttatt tgttttttta ttataaaaaa acacttattt
    24421 gacaaggatg taaacgactt tttggtttat caacaagttt atccactatt gacaaataga
    24481 ttatccacaa acagtggaaa acttgaggat aaactcagac aaaataagaa tgtatgaaaa
    24541 aggggttgtg gataaactgt ggaaacgaaa cgatatgatg aaacagaatt gcaagaggca
    24601 gccgaagcac tgaaagaagg agaacttgtt gcttttccta ccgaaacagt atacggattg
    24661 ggcgccaacg ctcttttacc aaacgctgtc aagaaagtgt tttctgtaaa agggagaccg
    24721 caagataatc cattaatcgt ccatgtcgcc tcttttgaac aagtaaaaga gtatgtggac
    24781 aactttcatc cggcagcaga gaagattgtg aagaacttct ggcctggacc attaactttg
    24841 atatttaaga tcaaaaaaga cactttgccc tcagttgtga cgggaggatt atccacagct
    24901 gcatttcgta taccggataa caaaaaaaca ctagaagtaa tcgaactttc cggtgttccg
    24961 cttgtcggtc caagtgcaaa tacgtcggga aaaccaagtc caacgactgc agatcatgta
    25021 tatcatgatc tacaaggaaa aattaccggg attattgatg atggagctac gcgaattggt
    25081 gtagaatcca ctgttcttga tttaagtgat ccaacagcca tgcctatgat tttacgacca
    25141 ggtgcagtaa cgaaagaaca aatcgaagca gtcatcgaat ctcctgttgc tattgatcag
    25201 catcttgtaa aagaaaatga aacaccaaaa gcccctggga tgaaatacaa acactactca
    25261 cccgatacac gtgttctgat ggtcagagat ggcgattggt caactgcagt tcaatgggca
    25321 aaaaataaga agatccgtgc aggtgtcata gcaagccccg aaattgcgga tcaagtgcgg
    25381 acagatactg cagctgttta tatgtataac gacaactcag tagaagcggc ggctaaagga
    25441 ttgtttgcag gtctaagagg attagatgaa ccaacacttg gattagattt gatttttgta
    25501 caagtgtatc cagaaactgg attggggaat gcttacatga atcgcttgaa aaaagcagca
    25561 ggacaaaatt attttgaaaa atgatcaatt ggttattgtc gcattctttt agtatgatac
    25621 aatgctatca agtaaatctt ctatcagtga acgaaaaact aagcgtaact aatcgtaata
    25681 gaagagatac gaatatttgg caatttgttt ttgatgggaa aaataaaatt gtaggaaaat
    25741 aaaattgtag gaaaataaaa aggagctgtt gtttgtggta gattacaaaa cgtttgaccc
    25801 tgatctttgg gctgcgatcg ccaaagaaga agaaagacaa gaacataatt tggagctgat
    25861 tgcctctgaa aactttgtct ccgaagcagt gatggctgca caaggaagta tcttgacaaa
    25921 taaatacgca gaaggttatc cagggcatcg ctattatggt gggtgtgaat ttgtcgatat
    25981 cgttgaaaat ttagcgattg accgtgcaaa agaattattc ggagcgaaat ttgcgaacgt
    26041 ccagcctcac tccggctctc aagccaacac ggctgcttat ctcgctttgg tagaaccagg
    26101 ggacacgatc ttaggaatgg atctttccgc aggcggacat ctaacacatg gatcaccagt
    26161 caacttcagt ggcaaaacct atcactttgt tgcttacggt gttgatccga caacagaagt
    26221 tattgattat aatgtggtac gtattttagc tagaaaacat caaccaaaat tgatcgtagc
    26281 aggtgccagt gcttacggac gaacgatcga ctttgcgaaa ttcagagaaa tcgcagatga
    26341 agtaggcgca aaactgatgg tagatatggc acatatcgca ggattagtag cagcaggact
    26401 tcatcctaat ccagttcctt atgcagatat cacaacgact accactcata aaacattgcg
    26461 tggccctcga ggcggcatga ttttgacaaa cgatgaagca ttagcaaaaa aaataaatag
    26521 tgcggtattt cctggtattc aaggcggccc tctagagcat gtgattgcgg gtaaagcagt
    26581 agcattcaaa gaagcattag atcctgcatt taaagaatat agtgaacaaa tcatcgccaa
    26641 tgcaaaagca atggtcaaag ttttcaatca agcaattgga accagagtca tttcaggggc
    26701 gacagataat catctgatgt tgatcgatgt tcgcgagtta ggtataaatg gaaaagaagc
    26761 agagagcatt ttggacagtg tgaatatcac tgtaaataaa aactcgattc cttttgaaac
    26821 actaagtcca ttcaaaacaa gcggtattcg aatcggcacg ccagcaatca ctactcgcgg
    26881 gttcaaagaa gaagatgctg taaaagtggc tgaactagtt gtcaaagcat tacaagcaaa
    26941 agatgataac gctcaactag acgaagtcaa aacaggtgta cgcgaattaa ctgaaaaatt
    27001 tccgttgcat aaaaaataaa aatttaaatt ccattagtaa acagaaaagt tcaatgttgg
    27061 gcttttctgt ttattttttg ggaaaacgga ttctttgaca atatcggtga tgctctctag
    27121 tatatgaggt gtaattttag tcttcacact aaatctcagg gaggaaaagt catgactgat
    27181 cgtatctcgt tagaaaagga agcagtagca ttcagtgatg caaatgcgcc tcatccgcgt
    27241 atctatgaac tagcaccgga agaaggaaga gttttactag aagaagtaca agaatcacct
    27301 gtgaacaaat attccgtcga tattgaagac ttgatggtgg atactggcga atggggaaaa
    27361 ctcaatattc gtctcgtacg accagaaggg aatatggata aattaccagt tattttttat
    27421 attcatggag caggctgggt ttttggcagt gctcaaaccc atgataaatt gatcagagaa
    27481 cttgcggttc gtacgaattc cgtcgttgtc tttccagaat atagtcgatc accagaagca
    27541 aaatatccaa cagcaattga acaaaattat gctgtgttgc aacagctgaa taaattagcc
    27601 aagtcaaaaa atctggatct cgagaatcta atcgttgcag gagattcagt aggtggtaac
    27661 atggctacag tcatgacgat cttaacaaaa gaacgaaagg gcctcccaat atccaagcaa
    27721 ttgctgtatt atccagtcac aaacgctgat tttgatacag attcctatca tgaattcgga
    27781 gaaaactatt ttttgacaag agaaggaatg atgtggtttt gggaccaata tacgacgaat
    27841 gaagaagagc gtgctgaaat cacagcttcg cctttgagag caaatataaa tgaactaaat
    27901 gatcttcctt cagcaatgat tttaacggga gaagcggatg tattaagaga tgaaggtgag
    27961 gcttatgcgc gcaaacttcg tgatgcaggg gttcctgtta cacaagtacg cttccaagga
    28021 atcatccatg attttgtcat ggtaaatgct atggatcaga caaaggcaac gcgtgcagca
    28081 atggcattat ctacttcttg gatcaagaaa aaagaataag ctttgcttgt ccgcaggaaa
    28141 acttcttttc aaaaacggat tttcttagga aaaatggcga gtttgactag tcaatcactt
    28201 gattttgttt tacaattaaa aagaaacgac agaacaaagg agagcttaaa aaatgggcaa
    28261 atttcaagtg atcgatcatc cattgattca acacaaacta actattattc gggacaaaaa
    28321 ctgcggaaca aaggtttttc gtgaagtagt cgatgaaatc gctatgttga tggcatatga
    28381 agtatcacgg gatatgccat tggaagacat cgtgatcgaa acaccgatca ctgaaacaac
    28441 acaaaaaacg ttgtctggta aaaaagtagc gattattcca atcttgcgcg caggtattgg
    28501 aatggttgac ggtatcttgg aattgattcc agctgctaaa gtgggacata tcggtcttta
    28561 tcgagatgaa gaaacattgg aaccacatga atatttcgtc aaacttccag aagacattga
    28621 tgcacgtcag ttgtttgtag tagatccaat gttggcaaca ggcggatcag caatcatggc
    28681 aatcgatgca ttgaaagaac gtggcgcgtc gaacattaaa tttgtttgtt tggtagcagc
    28741 acctgaggga gtaaaagctt tgcaagaagc gcatcctgat attgacatct atacagcttc
    28801 tctagatgaa aaattgaatg aacatggtta tatcgttcct ggactagggg atgcgggtga
    28861 ccgtttgttt ggtacaaaat aagtaaatac gaaaaagggt gtgacacaac ttttgtcaca
    28921 tccttttttg gtcattttta acgtttgatg cagaacagct ttttcacagc tttcgtttgc
    28981 atataaaaaa accatcccaa aaatgcagag atggtcaaat tcaaatcttt tttagttctt
    29041 tgtagttaac gattactaca cccttacttt gcgtcatcaa gttcgcattt tctgcagttt
    29101 catcaatctc aacaacagcg gaatttttca attgctttga aatcgttcca gtttgcgact
    29161 tgccgtgaat tgtgaacatt acgtgagtat tgatcttgaa tttttcacca atttccgatg
    29221 gtacgatagt tgtatcaaat tttccaaaag ttgccatttt cataaaacct gcctttcaca
    29281 tcaattatac cacgttttat gaaaaatatc atgaaattaa actaattttc catcggactc
    29341 tttacaatca gccaaataga gtatataatt tcttttggat aaggccagct tagctcagtt
    29401 ggtagagcaa cgcactcgta acgcgtaggt cgtaggttcg atccctatag ctggcattaa
    29461 aagagaagac agatatatca aggattttta atccattgat acatctgtct ttttgtttaa
    29521 aggaaataaa gtatgctttc ttagcaatag agcatgtatt atttctataa aaatttagga
    29581 gaataaaacg actagcaata tagctgtgaa atcaaatatc acatgaactg cccaacttgt
    29641 aatgatcgag ttgcttttaa tggcagcttg gttgaataac aatcttgcca ccccttgtat
    29701 caaaaggatg tgtgctacag tgtgtaaaat atttccatta tcataagtac tgtaatggat
    29761 aaatccaaag atgattgctg acaagaaact agcgacagga atgctgaact tactagaaaa
    29821 aatagcgaga aaatacactg agaacaattc ttctccaagt atcatgatag gcaaaataat
    29881 aaataataac gggctgaaag cctcatttat tggattgcct gttaaacccc atttcaaaat
    29941 ttgttgcaaa aataccgaga ctgcggcact gattactaca ttgataaaaa aatatttggc
    30001 tatatttttg acaggagcga caggtttcga gaacattttt ttcgtatctt ttttaccaaa
    30061 aacaagtgta aatgctataa atccagtgat agttaaagaa aaaatgctaa taatggatgg
    30121 cgcaaatagc aaccctaaca aaccgagtac aacgacaata gtgcccaata tccctttttt
    30181 aagttctatt ttttttccta aaatgttatt catgataata actcctacta ttcagt
//
LOCUS       DANKKG010000020        29363 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000020 DANKKG010000000
VERSION     DANKKG010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.020"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(46..270)
                     /locus_tag="PW004_000771"
     CDS             complement(46..270)
                     /locus_tag="PW004_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725762.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(290..712)
                     /locus_tag="PW004_000772"
     CDS             complement(290..712)
                     /locus_tag="PW004_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HDL2725763.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(854..1564)
                     /locus_tag="PW004_000773"
     CDS             complement(854..1564)
                     /locus_tag="PW004_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725764.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(1582..2079)
                     /locus_tag="PW004_000774"
     CDS             complement(1582..2079)
                     /locus_tag="PW004_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2725765.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(2116..2568)
                     /locus_tag="PW004_000775"
     CDS             complement(2116..2568)
                     /locus_tag="PW004_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL2725766.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(2703..3308)
                     /locus_tag="PW004_000776"
     CDS             complement(2703..3308)
                     /locus_tag="PW004_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDL2725767.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(3758..4378)
                     /locus_tag="PW004_000777"
     CDS             complement(3758..4378)
                     /locus_tag="PW004_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HDL2725768.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(4380..5249)
                     /locus_tag="PW004_000778"
     CDS             complement(4380..5249)
                     /locus_tag="PW004_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HDL2725769.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(5272..5679)
                     /locus_tag="PW004_000779"
     CDS             complement(5272..5679)
                     /locus_tag="PW004_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDL2725770.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(5698..6987)
                     /locus_tag="PW004_000780"
     CDS             complement(5698..6987)
                     /locus_tag="PW004_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2725771.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(7014..7316)
                     /locus_tag="PW004_000781"
     CDS             complement(7014..7316)
                     /locus_tag="PW004_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2725772.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(7341..9434)
                     /locus_tag="PW004_000782"
     CDS             complement(7341..9434)
                     /locus_tag="PW004_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDL2725773.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(9923..10687)
                     /locus_tag="PW004_000783"
     CDS             complement(9923..10687)
                     /locus_tag="PW004_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HDL2725774.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(10798..11667)
                     /gene="mdcB"
                     /locus_tag="PW004_000784"
     CDS             complement(10798..11667)
                     /gene="mdcB"
                     /locus_tag="PW004_000784"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HDL2725775.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(11692..12768)
                     /locus_tag="PW004_000785"
     CDS             complement(11692..12768)
                     /locus_tag="PW004_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HDL2725776.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(12792..13385)
                     /locus_tag="PW004_000786"
     CDS             complement(12792..13385)
                     /locus_tag="PW004_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HDL2725777.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(13389..15065)
                     /gene="mdcD"
                     /locus_tag="PW004_000787"
     CDS             complement(13389..15065)
                     /gene="mdcD"
                     /locus_tag="PW004_000787"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HDL2725778.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(15055..15354)
                     /locus_tag="PW004_000788"
     CDS             complement(15055..15354)
                     /locus_tag="PW004_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HDL2725779.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(15456..17102)
                     /gene="mdcA"
                     /locus_tag="PW004_000789"
     CDS             complement(15456..17102)
                     /gene="mdcA"
                     /locus_tag="PW004_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HDL2725780.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(17109..18020)
                     /locus_tag="PW004_000790"
     CDS             complement(17109..18020)
                     /locus_tag="PW004_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDL2725781.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            18140..18997
                     /locus_tag="PW004_000791"
     CDS             18140..18997
                     /locus_tag="PW004_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL2725782.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(19184..19546)
                     /locus_tag="PW004_000792"
     CDS             complement(19184..19546)
                     /locus_tag="PW004_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HDL2725783.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            19892..21331
                     /locus_tag="PW004_000793"
     CDS             19892..21331
                     /locus_tag="PW004_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2725784.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(21338..23890)
                     /locus_tag="PW004_000794"
     CDS             complement(21338..23890)
                     /locus_tag="PW004_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HDL2725785.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(24004..25335)
                     /locus_tag="PW004_000795"
     CDS             complement(24004..25335)
                     /locus_tag="PW004_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2725786.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(25301..26779)
                     /locus_tag="PW004_000796"
     CDS             complement(25301..26779)
                     /locus_tag="PW004_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2725787.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(27084..28919)
                     /gene="tdc"
                     /locus_tag="PW004_000797"
     CDS             complement(27084..28919)
                     /gene="tdc"
                     /locus_tag="PW004_000797"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HDL2725788.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
BASE COUNT         8448 a         6231 c         4971 g         9713 t
ORIGIN      
        1 tttctttatt gtgttaataa agtttcttca ttctagtctc aaaaatcaat agtgtgattt
       61 gattttacca tttcgtttct gttcttcttt gatgaattct tttgtatcga caggattcag
      121 attatttagt ccgccaaaac ctgtatcgac catttttccc cagccttttg tcttcttttt
      181 tcctgatttg agattctttg ctcgaagctt ctcactatgt tttgctcgcc gatacttcaa
      241 ataaccttct aatcttccca ttagatccac tgtttttcct cctttttatt caatcagacg
      301 actcagcttt tcctataaca aaataattgc cttcagggtc agaaaaattg aaagtcaaga
      361 tgccattgtt atctgtgatt tctcctggtt cttccatgtt ctcgtagata gtttcaaatt
      421 catccgtaaa aaatactaaa gaaggcggtg gtcctaatac ctctggtgaa aacttctgca
      481 caaatacttt tgggaatacg cctaattcta attcttttga gatgctgagt acaatactct
      541 taaattcttc tggcaattcg attgtttcag aaatttctgc accaattttt tctacaaaga
      601 atcggcaaat catctctacg tcattgacat aaagcatcac acgtgttttt tgtatcatca
      661 tttgaccctt ctttctattt gattttcttt cctgtctata aactgattcc attatatcac
      721 gaaagcgcaa acaccttgtg tttgatacta ttcaagaaaa ataaagcaat agtttctagg
      781 attgctttcc tccacaaact ttttttacaa aaaaaaaacg gcaatgaatg ccgctcttct
      841 tatttcgatt cttttataat tctgcttctg attcatcttt ggttagtgtg tgtctaacac
      901 tagttacgcc aaatgtttga tcgatttttt ccttcaacgc ttcgattgcc tgtgcgccac
      961 gtgccgcttg ttcagcattg atggattcaa tgaccagcac cgctgcttgt gtttcttccg
     1021 tcagcatcgt tctttgggct tcacgccagt tgagacagcg acagatcgct ccttgttcat
     1081 catagtagat gatttcttcc ggaagtgccg gagcatcgga atctgcacct aaaggcagga
     1141 atgattcgcc accttgggct tttcctaaat gcagatttcc tgcgatcttt tcgatatctt
     1201 ctccgccgca aggtacgcca aaagacaagg aaatactatt atagatatct acaagcggat
     1261 tgattgggaa aaattcccgt tcttgatttg cacgtttcag taaagcttca atagaagaac
     1321 gtgcaccttt tttagtcttg aatgtggtaa atgcctgacg ccactgagca atgacttcgt
     1381 tttggctgaa tgtttcttct gtaaggtaat ttcttgcttc ttttgctgcc tcattcaata
     1441 attgatgata cgtttcttca ctttcttttg ttacgtggtt atcgatacca tttacggata
     1501 agatttcgat ggttgcttca ggaaatactt cccagaaatt ggtatccact attacttttt
     1561 tcatctgttc acacctcttg attagaataa ttgcttgatg aatagatagt ctgtttgggg
     1621 atcatctccc atataaaaaa catgttcgct gtatttgata aatccttttt tacgataaaa
     1681 agcgagcgca acatcgttgt gttcccaaac accgagccaa atggctgttt tttgcttttc
     1741 ttttgctttt tcaatagctg tatctagtaa aaatgtaccg ataccttgat gtttaaattt
     1801 ttttcgtaca tagattcgct cgatttctaa aaaatgatca gccatttcct cagattgact
     1861 cgttcctgta tttagtttca ggtaacccgc aggctcttga tcgatactca ctaggaaaaa
     1921 atcggtttct ttatctttta gctcttctgt aagtttttct agtgcataag cttgtgtcaa
     1981 gtaatcagct aaatgctccg gtgaattatc ttttccaaat gtatctgtaa atgtttcgat
     2041 actaatttgt tgtagtaagt ctacatctga aggcgtcatt tttcgaattt cgtatttatt
     2101 cacggcttct tcctttcaat agatcctttt attgcctttt ttgacaaaat tccaatcttc
     2161 gctaacattc ttgcatactt tatttaaaag atattccaat tgttttgctt cttcgtcaga
     2221 aagaccattt aatgcgactg cattggagta gttgttttct cgaatgatga aaggatagat
     2281 ttctgctcct tttttagtcg gatagagttt cttgattttt ttattttctt tatcttccaa
     2341 acgttcgatc atcccattgg attctaattt cttgattgct cgggcagtcg ttgtacgatc
     2401 tactttgatc atttctgcta gtttttcttg gatgattcca ggattttcgc aaatacgtac
     2461 taaataaaga tactgtcctc ttgttaaatc gacttctttg aattcgatat tgctaataga
     2521 atctagcgct cgggcaatca ttccgatgtc tcgaaggatt tcagccattt catcccacct
     2581 ttattttgtt tgtaagacta gttcacctct ttttgttgca aatgcaacta ttttcaataa
     2641 aaaaatcaag ttctttttta ataactagta aagtatattc ttttcttctt ttacgaaaaa
     2701 gctcaatcac aatagatctg gtttttgttt tctaaaataa gtaactgttt cttcgttgtt
     2761 cgcttttctg atagctttgg caactcttct atactaaaaa atcccatgtt ggaagtttca
     2821 ttattctcaa taaacttggc ctcttcaccg tggatcgcac aagcaaaaac aagtttataa
     2881 tactgaaaaa gctgtggaat atctttttgt ttattcgtat caaaaactgc tcgcaactga
     2941 gtagtttcta ctgttaatcc tgtttcttca tagacttctt tacgcacatt ttcttcagga
     3001 gacaatccta tttcagcaaa tccaccagga agtgaccatt ctttcgtccg tttatcttcg
     3061 attagtagga ccttgccatc ttttttgata taggcacgaa catctacttt tggtgttgga
     3121 tgtccttctc caatcgttgt gagcgctttt atttcttcga aagtttcttt tcctatttca
     3181 ctgatcagtt ccaatgaaat cgtccggatc tcctcaaaac gctctttatc aaatacatct
     3241 tttccataat atagaccagc ttgagaaagt gcgagtagtc ttttgtattg agccaacttc
     3301 ttgttcattc tcttcccttc ttttttagca aatgtatgac tatgtaatca agtatagaag
     3361 actggtttaa ttacggcaaa taaatataat gaaatctgca ataaataaaa gaatgttaaa
     3421 aaactcgaga acaaaagaca ttcgacttct ctggggctgt gaataaatgg cgttcaaaag
     3481 cttgcatctg cggtaataag ccccaccaaa cgaaaaatat gacgagctat tcttgtctgg
     3541 ctgttcttat taattgaggc aggacagctt tttcacaacc tccccactaa acggtgttct
     3601 cctaccaact cctcggtcac aagtcacaat attcatcaaa catgagaagc tgtttttttg
     3661 aaattgctcc ttgtgactcg gagctgaacg acaggctcac gacctctcgg taaacagcgt
     3721 tcaaaagcca atttttcgat attaagccat ttctttgtta cttattgtca ttgatttctt
     3781 tttctaattt atccaatagt ggagtgtaat ccacttgagt atagtctgtt acatcaaatt
     3841 catatagtgt tcctaactgg aatttgtcat attttcggtc ggcgatgatc tttttgaatg
     3901 cttctttcgt aatatgattg gtccctttcg ttcgatcttc caatacgtca ccatagtgat
     3961 aatgatccat gatatagctc aaatgacgat ctgattcacg atcacgtttg taccgacgtt
     4021 gccataacac ttcaaagtct gctgttaggc gaatcgtgat cacctgatat ccgtatctat
     4081 ctgctaattc ttggagtcgt gctttttgct tatcactaaa aggatactcg gacacaatca
     4141 ctttctttcc tgcttccata tacaaatcaa gtgctttgta atagaaagga taaactttcg
     4201 tcctttcttg ttccgcccgt tcttccaaat tgttaaaccc gatggattct gcatagtatt
     4261 ccttgatttc atcgagcgtg atggcataca tatctggaaa tcgttcccgt agtttattca
     4321 taagatatgt ttttcctgta ccaggactac cagccagaag aatgaaatac tttttcatct
     4381 catttatttc ctaagttaat tggactttcc aagttcataa acagtccaat cttatgcttg
     4441 acaatttctt ttactgcttc atttgctgga atcacattat ggcgaagaga cttgttgatt
     4501 tctgtttcac taaaggtttc tttagctact ttcgtccagt tgactaaaag ctccgttcca
     4561 acattgaatt tgcggatccc gttattgatc aattcaggat aatcttcttc tttcacacct
     4621 gttcctccat ggataactag ggggacatca actactgcat cgatttcttt cagcaaggga
     4681 atgtttactt ctgtttttga tttgaattgt ccatgattcg taccgatagc aatagctaat
     4741 gcatccacgc ctgtagcttc tacaaatttc actgcgtctt ccggtcttgt atataccttg
     4801 tcattttcag cgacatggat tccttcttct gtaccgccga ttgttccaag ttctccttct
     4861 acggaaacac cgtgcgcatg ggcatattct actactgctt ttgtcttcag gatattttct
     4921 ttgaaaggca gatgggaccc atcatacatg acagaagtat atccgctgtc gattgcttct
     4981 ttgatatcat caaagtctcg ggcgtggtct aagtgcaaga ctgcatcaac catttcttct
     5041 tctgcaatag ctgtcattac attgactaac acatgcatgc cgatatattt agcggtatcg
     5101 acacttgttt gaataatgat cggcgaaccc atttcttttg ctgcgcctag catagcagga
     5161 agcatttcta ggttatgcgt attaaaagct ccaactgtaa aattcaactc ttctgctgtt
     5221 tttgttactt ctttcaatgt tgtatacatc ttttattgcc accttatctt tttatttgtt
     5281 tcctttgatc gttacttgtt ttgcaatttg tcgcatctca tccattttgc ccatgtagta
     5341 atcgatccct tgatcatcac cagcacgagc agcatctgca cgcttctggt tatagaggct
     5401 catcagtttt ttatacccat ctccgatcgt ctgtttattt tccaaactct tttctaaata
     5461 ttggatggct tgatcgttca catacaattc agaaaattta agtccaactt gttcataaag
     5521 tttcgctaat ttatgcttat cttgggtagt ggaaatctct tgtttcaatt gtgtaatctc
     5581 tgattgaatc ttttcggtcg cttctggcga tagggctttt tcttcttttg ccagaacttt
     5641 tttcggttct ttttcttttt tcttaaaaaa tccaaacatc attttcccta ctttcaatta
     5701 aaatgctttc aaaactgggc ttaagatcca ggcatataag agtgctgcgg ctactgtatc
     5761 tacatcggtt gctgtagcat tgttgaatcc catcgaatta aagatcgtta ctagcaaagc
     5821 gggcaataat gtaataaaga aaccatgagc aataccgcca atgatcgctc ctcggcgtcc
     5881 gccaactgcg ttgccaaata tccctgctgt ccctccggca aagaaattgg ttagcatacc
     5941 aggtaaaatc atcgctaagc cgaacatggg caaagtaaac atggcaataa tcgtaccaat
     6001 cgttgtcgta atgaaaccta aaatgacagc atttgggcta taaggaaaga aaactggaca
     6061 atctaaggca gggatcgcat caggaactaa tttcatagct atccctcgaa atgccggcac
     6121 gatttcacct aacagtagac gtacaccagc tagcaataca tacacaccca ctacgaattg
     6181 gatggcttgt aaaaaggcaa acatgatata gttttgatct cctgataatt cagaagcgaa
     6241 tggttcgcct gcaaataaaa cagtgatgat atatagcggg accatcacga ccatgacaga
     6301 caaataagta tcttgcagaa attcgaaatg tgtcggcaac ttgatatctt cgatcgactt
     6361 tttattttcg cctttctcac caaaaagctt agcaacaccg gcttcaaata agtacccgat
     6421 cgtacaaaaa tgacctaaag caatatcatt tgatccagtg attttacgaa tgatcggttg
     6481 cgctactgcc ggcatagcaa tggcgaaaca agctccgata aaaccaccga ccaagatcaa
     6541 tacgatccct cgcagaccgg caaaatagcc aaagactgtc gtcatcgttg ccatccataa
     6601 aattgcttga ccagttaaaa aaatgtactt ccattttgtg aaacgagcca aaataatatt
     6661 gaatacaaaa atggctaaga acgtcagtgc aatatctcta cctaaaccca aatcattcat
     6721 tgcttgacca ttgatagatt caatagaagg gatgatccct tgcatatgaa atccttcagt
     6781 aaagattttc ccgaaataag ttaaacttcc aacgatgatg ctcgaaccgg cactcaacac
     6841 ttgaaatcct aggagtgtct ttaacgtgcc agagatgact tgtcctgctg atttcttctg
     6901 cagtagtaga cccaacatag caatcaatgc aatggtgatc gatgcctgag tcaaaatatt
     6961 ttcgatgata aaattgataa cgcccatgtt tttaccctct tttctttttg tatttagata
     7021 actcccttat cttgcagaac tggggtgatt ttttcttcaa tttcattttt tgaaacaata
     7081 tttttcaaat agatcatcgc cgttttagga tcaactgcga acttttcgaa ctgcgaacga
     7141 aaattttctg ctgtgatgat cacatctggt ttcatggaaa ctgcggaaga aatatcacaa
     7201 tggtccagtt tggcttctac tcctaatttg ttcaaaacat cctcagcaga catctgtgct
     7261 gcaaaactac ttcctaaacc tgcaccgcat acgaataaaa tgttcaattt gttcatcttt
     7321 gttctccccc gtatcattta ttatttctca actactgaaa aaagcagttg tttgaaatct
     7381 tcgatatttg tgcattcaat caatcgatcc aacttctctt cgttgttgat caattcaatt
     7441 agttctttca tgatgttcag atgcgagtag gagtctactg ctgccaaaca aaaaataatc
     7501 tttactggat ccatttcagg attaccgaaa tcgattggtt gctcaatcgt agctacgctg
     7561 actcctaatt tattgacccc atcttctggt cgggcatggg ctagtgccat gtgtttgcca
     7621 atcacgatat acggtccgta atcattcact gcacgaatca tcgcttcgac atagcgctct
     7681 tcgacaatgt tttctttaac caatggtcta gcagctagtt caatcgattc ctcccaagta
     7741 gtggcttttt ctttgatcat gatattgctg tccgtcaaaa tatcttttaa cataggctgg
     7801 atctcccttt tgttgatttc taaattgttt gtttcgaata atttttctaa cttgccatag
     7861 atgattcgat ctactcggcc accactttct ttcatgacat ctattatttg agtgaataac
     7921 tccgtcgatt catgactgtt aggctctaac cgctgtagtt cttcatttct tttcaaaaag
     7981 tcttctataa ccaagatatt cgtctcagtt aaaatcggat cgacaactaa aagcggctta
     8041 tttcgataat tcaattgaaa ggtgctgaaa accagattga catccaattt atctactaat
     8101 tcgatttctt ttgaacttaa aactgcagta atctcgatct gaggaaactt ttctttcaga
     8161 ttctctgcta acagatttcc agtagccatc ccatggttac agacaacgac tgatttataa
     8221 acataattca agtctcgctt gattacactg gcgatcgtcg aaaagtggat gacaagaaaa
     8281 gctatctcgt cctccagtat cttgccacca attacttctt cgatcgtagg aacaaaatga
     8341 gtgactgcgc ggtagattgg gaaataattt gttcggatgt tttctttcaa tggattcatg
     8401 acttggatat gattcttgat tcttacaatc agtgcagcca tgtgcttata gagattttcg
     8461 aataaagctt cttcctttag atggaaaggt acgtgtgtct cgttctctac aaattggatc
     8521 aattggatcg ttagtaattg agcttgtacc cactcgattg aattggagat atctctagca
     8581 ttgaacgtct caatagtgaa acgaagataa tttctttcta cttgcgggac attgtccaat
     8641 tgaaattcct cgatcatgtg gtcaataaat gcagaaaagc tactgtcttt tatttctgta
     8701 tttttccaat taatggcaga gataaattca tgtttcctga ttcgctggat ccagatcaat
     8761 gtaaaaagca ggatttgttt tttataaata tcatcttctt gtttcaacaa cttattggaa
     8821 tagatctcct gtattttttt gatttccacc aaaggaaaat aatggagaaa gattttttga
     8881 gccggtgtta attgtgcatc ttgattcata aagaagtcgg tcattcccag attctgattg
     8941 ataaaatcat agatcatcgt acggatagat cgttctgaac ctttataaac cagtccttgc
     9001 tttccataac tgacgatctc tatcccataa ctgaccagtg ttgctcgtaa ccgtctcata
     9061 tcttcatcta atgtactttt agagatttga aattcttctt cttttttgtt tagatggatc
     9121 gggattcggc tgtaagcaac ggctaaaact agatcaaaaa tacgttcgcg ccgattcaaa
     9181 aagccgtctt tcacatcttt tatttcagtt tctaatcgag cttgcttttc cttttccagc
     9241 ttcaacaaaa aacctttgtt gcgaattgta cggatctccg gaagcttttt actgctcaac
     9301 cagatattcg cttcaagaac ttcctgacgg atcgtccgct gactgacatt gaactcactt
     9361 gctagatgtt ttgcttttac tggatattgg gctgtcatga ttctgtgaag tatttcaatt
     9421 gttcttgttt tcatagaaat gatctccttc tgatttcaag tatatggttc cgcttacata
     9481 ataaaaagta gatatttctg ccacttgaag tggaaagaaa gtaagcaaag caaacaaaaa
     9541 ataagccata tgctttttca tctcctcaac gaaaaagaca tacgcctatt attacaaaaa
     9601 ttttgcccat ctatacaaaa aagaggttgt gaaaaagctg tcctgcatca agtaataaga
     9661 acaactaaac taaaatagtg cttcctattt ttcgtttggt tgttcttatt atcgcagatg
     9721 caagctttta aacaccattt agtggagagg ttgtgacaga gatgttcagc cttgagcgat
     9781 aagaagcgat tttgaaaaat cgcttcccat attttacaaa atcgtgcctt attgccgata
     9841 ggctatctct ggcacaccgt ttattcagaa aaaggagctg cgacaaggct tttgtcacaa
     9901 ctccgttcat aaatatttcc tgtcaatcca gcataatcgg ctgaataaaa tcggcttgcc
     9961 atcttttttc tttctcagaa aaatcaaaat accatatctg agaaaaatac tgtcgttgtg
    10021 tcttacttcc tgagatcaat cgcccatctt ttcgatcgac tgaataatca cagcatctga
    10081 aatcaattcg gatggcaaaa ctattttcgt ggatctgtcg ataatttcct acttcctgaa
    10141 tagttatttt tttagtatga tttcttacac cgtctgcttt gtttttttgc agaactttct
    10201 gatgttcggc aaacagcttg tctgtataac agcttctggc tttttcagga gtttcttctt
    10261 cccaagcgga ttggatcttc atgaatatct tttggatttc cagaaactgt gctttctttt
    10321 ctttagccgt tcctgacatg cgggacaata tctccgcttt cagccttttc ttttgtcttt
    10381 tattcttgac gatagaaaaa gcagaaaccc ctcctactga caaaacagct aatgcaagaa
    10441 atcccgtttg ctcataacta gaaggacctg tacctggacc ataataccca taagaatctc
    10501 tgccgtaaga acgactgcca aaataactgc ccccatatga acgaccacca aatgaacctc
    10561 tgcccgaact tcgaccgcta gatgtacctc caccatgacc acccgcgaca gcgaatcctt
    10621 gggtcggcag agcaaacaaa cataggccca ctaacaaaat gattgctaaa taattgattt
    10681 ttttcattgt cttcctcgtt atcttttatt acaaaaaact atttttatga tagccttgct
    10741 aaaaggaaga gtcaataacc agatgcataa actgtttttt tagcaatttt gtcttattta
    10801 gtcattgagt aacatcggac aaatgtatgt gtcggcagtc cataattcct ctatcttcag
    10861 catgaacaag cttgcagaca agagatctgc gcttcctcca ggagagattt ggttttcctg
    10921 acaaaagtgc aacagtgctg taaatggacg atttggtaaa gcactttgac tagcagcatg
    10981 agcaagtttt tgcatttcca ctaacgtttt ctgatcactg cgatgcaata cgcatgtgtc
    11041 atctagcgaa ctcatcaaaa ctaacaacat atgcatttgt ttctgtacat atgaatcatt
    11101 atctagctcc ttagctgctt gaattgcaag cctgatatgc ggatacccta atatcgcttc
    11161 ttcatatgca ccatttacgc catatttttg cttcacccta tgaccatgag tggttttttt
    11221 atgacgatat tttgtatcag gaaaagcagc aagcttgctg acatcatcaa gtattttcga
    11281 taaagaaaaa ggctgttcca tactgatttt ttgtgcaatg acactgatca atagccccat
    11341 tgcccaaatc gctcctttat gggtattgat gccatttgta gcaatcagca ttgccttttc
    11401 cgcttttcgc ccaatctcag caattctttc tcttaactcc tgatcaatag catgatgcca
    11461 tgctatttga gccatttctt caaaaaaagg agttagcgtc ttagcagact taagaaataa
    11521 agcatagtcc atatcggaat gtgcacccgc atccagtgca tccactaatc caggttttgg
    11581 acttaatgtt acttctgcga tcaatgcttc ctctaccaac gcagcgagtt gctgagtcac
    11641 tgcttttttt cgttcgtcat ttatcatgac actcactcct tcttcctact attacttgct
    11701 gaaacctttt ttgatagcta cccaaatgat atgaagtaac cagcttaaag caaatgcaca
    11761 acttgccgtc attagataag ctacaagaat catgactcct gaaggtaact gcagccaact
    11821 atctttaaac aataataaca gtacttggaa aacaaataca ttggctaaat acgtacgata
    11881 agcataaaca gacaaccatt tgaacaatgg caattttggc gaatcgttta caatcatttt
    11941 ggaagcgcct atgaagacag caaagatgat gactaaactg tataaacttt ggatcgtatt
    12001 caagtaaggt gcattcgcaa agctcaagtc tcctggatgt gcgagcaatg aacggacaga
    12061 aaagaacgcg ataactaagg caacaggtaa gaaaagataa cgtactctgt ccaaatattg
    12121 atagactttt tcgtgataaa caaatgcggc taccccataa atcatgaaac tagcaacaaa
    12181 ccgatctaat aagtaccagc tgtcatgata aggcccagtc aatacttgtg tttgatagaa
    12241 cagataccat gcgacatata aaatcgttga accaatcaat actggccata catatttttt
    12301 attagcaaat actttgtaac cgagccaaac aaaaaacggc atgaacaatt ggatctgcag
    12361 catcataacg gtataccata aatgaggcgc tccatcacct gtgacatatt tcagtaaaaa
    12421 gcttgcagta tccgtatacg gagtttgttg ttggactgct ggaaatagcc aaagataagc
    12481 cgtcgtccat agaagatacg gaacgactag ttcaaaaaac ttttctttca aaaattccca
    12541 ataagacaaa tgctgacttg tttttaccat attataaaca attgcaaaaa taaacattgg
    12601 tgctgtgtat ttaacgatga cgtaaaaggt accaagaaaa gctgtatttg ttgcgctgtg
    12661 gctgtttgcc aaagcaaagc tcaatatcgt ttgcatcata acagccgtta cagctgatac
    12721 tttctggaag tcacttacgt tgattttgat ttttttgttt tcaaacatct ctttctctct
    12781 ctttttcttt attaccaaag ttctttggtt aatttaggtc cttcgactgt tttcaataga
    12841 atttttttct ctggctctct caaatactct tttagtgaaa aaccgccttg ccgagtcact
    12901 acttgggtat cgaattgctc aaaaaaagca ggatgcgatt gaattacctg aattggcaat
    12961 tcaatcggtg aatttgcgta cagcagcaaa tcaaaatcac tggtttcttt tactgccgga
    13021 atcccagtcg ataattcaaa tcctaagctg ccgccgattc cccatttaca tcctgataat
    13081 atgtttctag cagcatgaaa ttgctgataa acaggataat ccatgatttt ctcttgtcta
    13141 tacgattctt gttcgatgat gtcccacgga tgaatgactt gtttgacggc atgttttgag
    13201 atttctgttg accatctctg attgcgggca ggtccacgta aaccaatcgg tagtatttct
    13261 gcgtgatccg tcgacgcacg acgaactgtt ccgaacatct tttcagttag aacgacccat
    13321 tcaggcaagg tgaacccttc tatttcatgt ttatcaaaaa ggacaatatc atgtgcttga
    13381 atcatttttt atgcatccca ttcttcgtcc attttggcac ggactttatt tgtttcgaca
    13441 cgtccgaatg ttttggctgt ttcgtttgtg tatcgatttg tcagcatcgt gtcatttgta
    13501 ttgcggacat cttgaatggc ttcattgatt gcttcgatga ctttgctgac attttgttct
    13561 gaagcagctt ggtcggtgat atcttctagg aaatgataga tcgctcctag ttttgtataa
    13621 ttattgatat cgtaagcgat agctggaact ttttcagcag ctgcttcgat ttcagcaacc
    13681 gaacgttttg tcacacgtgc agcactttct ttagacatcg cttggacggt aatttcttta
    13741 ttgtttaaag caatcaagcg gcttgattgt aatccatgtg ctaaaaatgc acctgaaata
    13801 gcatttcctg caatcaatcc aataactgga tgtccagctt gacggagttt tgcataagca
    13861 gcagcactat tagccaaagc cacatggata ccgatcaatt cttcgttata gccgtatgct
    13921 tggcttggta catcgatcac aacgatgatt ggccgtttca cttttttgtc tttatcttct
    13981 tcgtaaacgt gatccaaaat ttgtcccatg cggaaacctt caactaaacc aacttcaccg
    14041 tttcgtacac ggtacatcgg gtttttagga tcaggtacga ttgatgtcac gatggcttct
    14101 tctatgaatt tacttgattt accataatac acagtactaa tagaagaagt tggatttttc
    14161 atgccagtta gtgccgcaaa ccaatcgtaa ccaacacttg ctgcagctgg ttcatttcct
    14221 tctgctactt ctggtaagtc aattgatttt gcttgatggt tagcaattgc ttcattgtaa
    14281 tcgtcaatgg ccattggttt tgaaagatct aaagaatcaa gtaatgataa ataaaatgct
    14341 gagctttcac tgcgatgcgt gtcttttcgt tcgtccattg ctgcgatgac agcagctttg
    14401 attgcttcta cactatcagt cactaattca tcaatgattt ttgattctac ccgttgacga
    14461 gatcctaatg tattccaaat caagtctttg tcactagaat caaattctcg aacgcctgct
    14521 tcttgttcaa taacttctgg tccattcaaa ccgactctcg ctttttttgt tgcgattaaa
    14581 taactcatta atgctgaagt aatcgacata ccgccaaatg atcctacacg tcctggaatc
    14641 aaaccgatga ctggtacata ttgttttaga gcaatgatta catttccgat ttctgaaata
    14701 gataacagtc cgtagtttgc ttcttgcaaa cgaacaccac ctgtatctag tacaatgact
    14761 ggaaagatct catttccttt tttattttct tctaacacat gttcaagtgc agcaacgatt
    14821 tttgcaccgg aaacttcacc gataccgcca ccttggaatt ttccttccat agcgattact
    14881 actgcttgtt tgccattgat cgtgccacgg ctgacaacga tcccgtcatc actttcaggg
    14941 acgattcctt gtgctggtaa acttggcgcg atgatttgat cgaatggatc aatcaattca
    15001 cgtccattgt caagtagagc gttcactcgt tctctggcgt ttaattctac aaaactattt
    15061 ttcatctgaa agttcctcca ttgcttgcgt caatcgtagg tacacaactc ctggtgtagc
    15121 accgaagtca ttgatcacga tatctgctga aattggataa cgatcaaaaa atctcgttaa
    15181 tacattgttc catacttcgc cgaagccatc gcttcctgta caaattttta cagtagattc
    15241 tgttttttct gttggaaaca tcaagatttc caaatctcct gaaccgacca ctcctacatg
    15301 gattttctta ttgatcggct gtttcgttgg atatttataa gctaattgtt ccatggatct
    15361 ctcccctttt tgtaaaacag agcatacacg ttctgcttat tcgtctttct tcttccatct
    15421 gggtaaatgg cgacaaaagg tttttctcgc caattttatg accaagttct gaatttcatt
    15481 ggtggttcat acaggccatc tgaccattcg atcaaatcat cgatggtttg tgcagctaat
    15541 aatgaacgtt tcgcttcact acgtttgatg cctagatctt ctggtaattt cacgatgccg
    15601 gctgcacgca agctatctag tgattctttc gtgctagtca ttccaagagg tgtaacaccg
    15661 ccaattgctg caattgctgc ttgtcgttct tcttttgttt ttgctttgta caaataagca
    15721 attccttctt ctgtcacgat atgtgtagta tcttcgctat aaatcatcac tggtgcattg
    15781 gctaagcctg cttgtttttg aacagctagt gcatctaatt cttcgacaaa gactggtttt
    15841 ttatttgaac catatgtttc aaccatttga acaactaatt tttggccttt ccctaatgga
    15901 tcatcattgt cgcgaagtga ttgccatgct ggtgtagaat gacgacgacc gccaggatta
    15961 tgtcccatgt taggcgcacc gccaaaacca gataaacgtc cttttgttac ggtagaagag
    16021 tttccttcgt aatctaattg caaggttgaa ccgataaaca tatcgatggc atattgtcct
    16081 gcagcttgtg ataatgttcg gttactgcgc atcgtgccgt ctttaccgac aaaaaagaca
    16141 tctgggcgag cagcaatata tttttccata ccaacttcgc caccaaaact atggatgctt
    16201 tcaacccaac ctgattcgat ggctggaatc atcgttggat gtgggttcaa tacccagttc
    16261 ttcgcgattt tcccttttaa ccctagtgat tcaccataag ttggcagtaa caattcgata
    16321 gctgctgtat tgaatccaat accgtgattc aaagattgta cgccgtgttc tgcgtagatt
    16381 cctttgattg ccatcattgc cattagaatt tgcaattctg tgatgttttg aggatctcgt
    16441 gtgaataaag cttctaattg ataaggttta tctgctttca cgacaacgtc tacccagtca
    16501 ccagggatat ctacacgtgg taaagaatcg acaacttcgt taacttgaac gatcacgata
    16561 ccatctttga aagctgcagc ttcgatgatt gttggtgttt cttccgtatt gaaacctgtg
    16621 tacaagttgc cgtctttatc tgctttatct gcagctacta aaacaacatt tggaatcaaa
    16681 tcgatgaata agcgtccata aagttccaaa taagtatgga tatcacctaa tttcattgtg
    16741 ccgtcttcaa tcatttgggc gacacgcaag ctttgtgcac cagcataaga gaaatcgatc
    16801 ttgcttgcga ttcctttttc aaaaatattc aaatgttctg gtcgtgagat actagacatg
    16861 atcaaatgca gatcattcac tttttctgca tctacttttt caagactttc tgaaagaaaa
    16921 cttgcttgtt tttgattgtc gccttctaat acaactttgt cgccgggagc aatcagtttt
    16981 tccattactt ctacgatttt ttcagtttct acatacttgc cattgagcaa gttgctgatt
    17041 ttttccaatc gctgcgcttt tttttcgcgt ttagaagccc agttgcggtt tggtttttcc
    17101 ataattagtc atctccattt ttgatataga aacgtggcgt catcaatacc atgttggtct
    17161 agattgatcg tacggatccc tttatctaca ccaaattttg tatgaatcag ctttgtcaaa
    17221 gtattgccgg ggggaaattc aatcgtcaga tccattccta attcttttgc caagtccatc
    17281 atttggttcc actgcacagg gaaagctagg ttttccaaca aatctttacg aatctcttct
    17341 gcgtcctgag tggctcttcc atccgtattt tttatataca aacatctagc atcagataga
    17401 tggacagact gcatatacgg cttgaatttc ttgaccgtat cattcatcaa gagacagtga
    17461 gatggcacag ggactttcag cagcttcgct gtttgcgctt ggtgccgttt ggcttctgcc
    17521 aacgtaacgg ataaaccttc gatactccca gataacgtat gttgcatcgg acaattttca
    17581 tttgacagat aaactggatg gtcaggatca aatgtttttg ctacaatttc ctcagcttcg
    17641 cttctcgtca cacctactac tacgcccatt gcataaccgc ttggataagc ttctttcatt
    17701 aagactgcgc ggtgatagac tagttccgct gcattctcaa atctcaaaga tcgacaagca
    17761 acagctgcac aaaaagcacc aatcgaatgt cctgctgcaa tatcaggctg aataccgttt
    17821 ttttccattt catctaaata aaatactgct tttgtcagta atgctagctg gataaaaacc
    17881 gtatcttgat agttttcttc tttatcaact aaccgatacc ccgtcacatt ttctaaacgt
    17941 tccatgtaca tttcagggat atccgctaac atccctaact tctgactgcc ttgtccagga
    18001 aataagaaac ctgtactcat gagcctcgcc tcctcagcac ttactttttc cactgctatc
    18061 ttagtcaatt tctgctataa taaaaaatgc caaaaaaaga aaaaaccata acaaaaaggt
    18121 atggatggga tggataatta tgaaattgct tcatctgcag tattttaaaa aagtattcga
    18181 aacaaaaaat gttacccaag cagccaaaga attatttatt tctcaacctg ccttgagccg
    18241 tgcgatcaaa catttggaaa acgaattagg tgtccctttg ttttaccata gcggcagaaa
    18301 cattgagacg accgtttatg cagaagaatt ttatccatat gcagtcaaga cattagacac
    18361 attagaagaa ggaatcaaat tattgtcttc ggtcaatgag aagatcgtta cttccgtcat
    18421 attgtattta gaggtagcct ctgtttctat tccgaacttg gtccgaattt ttatggaaaa
    18481 gcatcctgat atccaattaa cgatcatgca acatcattta ccagatgata ttaccgatcc
    18541 tgtactgtac atcacttcgg agatgaagcc tggcatgacc aatattccta tcatgaaaga
    18601 acctgtttat gtcgccattc ctaagaaaca tcctcttgct aaaaaagaac atctgcaatt
    18661 agaagatatc caacgcaccc ccatcttgat gctgtcaaag agcaacgcct tccgtcatac
    18721 actggatgca gcacttgaag cgaaaagtat cgaattgacg atcagttcga cgacagatga
    18781 tcctgcgaca ttacgttcta ttgtcaagca aggattagct gttagctttt tcccaaaagt
    18841 ttcttggtct tacgataaaa atgatccttt cgtcttgcgg gaaatcaaag atttgccctt
    18901 gacacgaaca atttatttat cttctagctt cacagaagat catcctttga tcaaaatgat
    18961 tgcaaaaacg atgcgggaat tttatttagg aaaataaaaa aaaaagagct gggacaaaat
    19021 tcttgtcgca gctctttttt cgaacaaacg gtgttccaga agtaatttct tagaaaataa
    19081 gccgaaattt cccaaaaatt tgagaagcaa ttttcgtaaa ttccttctta tttcttgaag
    19141 ctaaacattt ctgtcacaac ctcttctcta ttgtcctaga cattcattgc gttgatttaa
    19201 aacttccatc aatcaaatct gctagttcat ctagcttttc tattttacgt agatcaatag
    19261 agatccaatg ttctttattc atgtgatagg ctggaaaata cccctctttt cctcgcaaaa
    19321 ttccgccaag ttcaggatcg accttcaaat caagaatatt gatgatccta tggtcacttg
    19381 catcatcata gattttttct tctggaacag acatcaggac cccaaaccat tttttgcttt
    19441 tttgatgtct gaatacgcaa taatcaggaa attttttaaa aatatattct ggagacaaat
    19501 tgtatgtttc attgatgtga tcgattagtt cttttttcgt tagcatctgc ttccatctct
    19561 ttcttattga ttcgctttat tcaaagatac cacgtaagaa tagaaaaatg aatacaagta
    19621 gttgctaatc aaatacttcg atatttttct tttttatcaa ctcttaaatg ttcaatccgc
    19681 ctgtgttggt cttgctacca tctactatta aaatgtttat tattgtttgc ccacctattt
    19741 tttgatttta gtggtatcat ttttcaagtt catgatgaaa ttcttatcat cagtaaatgc
    19801 tccagaaaaa taaacaatga ttgaaaaatc agttgaaact tgaccaccgt ttattttctc
    19861 cgtattctta gaatagaagg gagtctgatt gatgtattca ctaatgaaaa aaatcattac
    19921 cgaaaaagat attcgacgtc atgtttcttt agtagaacaa ttgctgaatc atacaaagat
    19981 aaccgtaaat gaattagctg aaatcatagg gacaaccgaa cgaacgattt tttctgacct
    20041 tcaatctatt cgctctcagc tgcccgaagg ctgggacatc atcagcgatc aagcaggaat
    20101 cagtttgcaa aaccagcaaa atttattaac aaatgacctt tgggagattt ttttcaaaca
    20161 atcagtcagt gtcgaactgc tgaaaaatct gctttttaca aaaaaagttg ctgtccctga
    20221 ttttctagcc gaccatgggc tttcttatgg gacattgaaa agacatgtga ctaaaatcaa
    20281 tcagcgttta gcaagttacg acttgcagat cgatttgacg aaatatacag cttgtattct
    20341 tggaaaagag cgtgcgattc gtacttttta tcatcgattg ctgattcctt tcactcataa
    20401 caactatttt ttcgaggatt attcgatcca tgaatcccat tattttcaat ttttaagaaa
    20461 cctcagtcaa actgaactag cggttgaaac agaagagatt ttcggaacat gttggttctt
    20521 tatcaatacg atccgtataa aagcgaattg ccgattagat tcttctatcc ataccaacag
    20581 cacactctcc tccctgtatg actctgcatt gaaaaaattg tatttaacgg aaggtattta
    20641 tcttaaagac actgagcttt cttttgcttc tttctgcttt ctggaaagtt ggaattacaa
    20701 caataactat ggacaagaaa ccgctcgctg cctgcatcat tctccatttt tagaggtcct
    20761 cgaaacgttt gtagaggaac tagcttcaga gctgtctctt gatcaactaa aaaaaacatc
    20821 tctgactgat aacttggctc tcctcatatt aaaatatcat gaatcgccga tattgattga
    20881 agcattggac cgtcaatacc atcgtttttt agaaacatat gatcagcaat atgcacactt
    20941 gtatcaacag aaagagaatt tactgaacga tctaaaaaaa gaaatagaaa tcaatgaacc
    21001 aaattacttt ctccaactct tgtctctact gatccaacaa acgatttttt ctatcaaacc
    21061 gaatttgttc aatgtctatt ttattttcca aggagaacct tcatggaagg cgttcttgca
    21121 gcaggaatta aaagattact taggaaaacg tgtctgcttc ttacctatcg aactaaccga
    21181 tctttccact atttcttttg aaaaaacaga tatcttgatt tccaatttcc ctctagattc
    21241 tctagatatc cctattgtat acatttcttc tattccaacg aaaaatgagc tcaatcgttt
    21301 gactgaactc actttcaaaa cttttttata gtaacggcta gcgactgtat tctctagctg
    21361 ttttttcttt tttgcgaaac agtttgttca tgatcccttt ttctatttct acaactaaga
    21421 atagaagaat cacatttgct ccaatcaata actgttgtgt catcgttaaa cttactgtac
    21481 cgatcagctg ctggccgact ggtagaaaaa taaccatccc ttgcaataaa gcaaggatac
    21541 ctaatgaaag aaaaagcgct ttgttttgca aaagaccttg gttcaatgac ggcttggaca
    21601 actctctgca attgatcatg taaacagctt gagctaagac gatattttgc agaagaatgg
    21661 tttgctgtaa gctttgccct tcaaactgca tagccaataa ataggcagga accatgatca
    21721 gtagggagac atagatgatc cgaaacaaat gataaccaga taggattcct tgctttggat
    21781 ctcttggtgg gcgcttcatg atttctggat ctggcgactc gaaccctagt gcataagata
    21841 aagtgattgt cgttaccata ttgacccata aaatctgtac tggggacaaa ggtagcgggc
    21901 gattcagcat aagtgcccaa atcaagatca atccttgagc taatgcagtt ggcaagaaga
    21961 aattgatggt tttttgcaaa ttatccaaaa tccgccggcc ctcttttacg gcttttgcaa
    22021 tcgtatggaa attatcatct cctaaaacca tatcagctgc ctgtttgctt acttcactac
    22081 ctttgattcc catagcaatt cctacatcgg atcttttcaa agcaggtgca tcatttacgc
    22141 cgtctccggt cattccgacg atttctccat ttttctgcaa agctttaaca attcgcagtt
    22201 tatgttctgg tgtcgttcga gcaaagacat cgactattgg cacttgctga atcaactctt
    22261 catcagacat aagatccaaa tcgatccctt ctaaaacttt ggcagtatgt tttaagccaa
    22321 tttgttccgc aatcgctttt gctgtgtctt tgtgatcccc tgtaatcatt ttcactgaga
    22381 tccctgcttg ttggcattcc tttacagctt tgactgcact ttcttttggc ggatcgatca
    22441 atccggcgat ccctgcaaaa gttaattggg ttaaagtctc atgcgtgagt tcctgtttcg
    22501 aatctacttt tttataagca aatcctaaaa cacgctgacc ttttctcgca aattctgcag
    22561 ctgtctgact ccatgcttct tgttctgcag aagaaagagt tgatttagca aataatactt
    22621 ctggtgcacc tttgacgtaa atcaccgctc ctttttcctc cgcatgcatc gttgccatat
    22681 atttgtattc agaactgaac ggaatctttg cttctatttt tttcaaaggt agattggctg
    22741 tttctgtgtg tcgaatcagc gcaacttctg tcggattgcc acttaaattt tctgttttct
    22801 gttcttcttt tgtttttaat tcctgacagt ttttcataat ggacaacacg caggcttctt
    22861 cttttgcagc tacatccaca actgtcattt cattttttgt caatgttcct gtcttatctg
    22921 aacagatcac tgtcatagaa cctaatgttt ctaccgaagg aagtcctttg atgatcgcct
    22981 tttcttttgc catttcatgt actccaacgg atagaatcat ggtcaaaaca gctggcaatc
    23041 cttcgggtac cattgacacc accaaagcaa tcgtagccga aagaagaata tgccaatcca
    23101 taccatatcg aaatgtcgta aaaataacta agaataggct caaacaaaga atgccttgaa
    23161 aaatttgttt gtttaatcgg ttgatttttt taatcagagg cgtcgtttgt tgttcgaccg
    23221 attgcaatgc ttgattgatt ttaccgattt ctgtcgtgtt tcctgtttct acaacgacac
    23281 cgactgcaga accagattgt acaagcgttc ctgaaaaacc catatttttc tggtcgccga
    23341 tcgattcttc ttctgtcaaa acggcatgac ttttttcaga tgcttctgat tctcccgtca
    23401 aaatcgattc ttccactaat aaatcatgtg cttcgattaa tcggatatct gctggaacga
    23461 catctccggc acgtaagaca atgacatctc cttttaccag ttctttcgca gggatttttt
    23521 cttgcatccc gtttgatagt actatcgctt cttgtcccat cagctgcttc aaaccattca
    23581 atgattcttc cgcttttctt tcttgccagt aactaacgag tccattgacg atcactacga
    23641 ataaaataat acttccttca atatattcac tagaagcaaa tttcaaaaaa gaggcaatga
    23701 tcaacacgat catcagcaga tcagtaaaat gctttgctat ttttttccat cgttttgtct
    23761 gctgccttgc ttttatttca ttatatcctt gatcagtcag gcgtttctgt cgttcttctt
    23821 gcgacaagcc ttctgttgat gtttgatagt ttttcaatac atcttctacc gtcaatctgt
    23881 accagttcat cctatcccct cctgtttaat tgatactgtt attatacgca gggaagtttt
    23941 ttcaggcgta tcgattattt caggggggtc tgaaataatt cgttttgcaa aatgtatctg
    24001 ggcctaactg atatttctat tgcttattct tttatataca aaatttaaga atacaaacca
    24061 gataaccaat aaaaaaggaa taaagcgtgt cgtgctttcg aataacaagc tgaaagcaat
    24121 tgaagtaaaa aacaccaaca aagcaagatc tttatatctt tgtgtagtgt tccgtttctt
    24181 ttctttaata aattgaacat gtgcacacaa gatcgaagac cacacaaata gaaaacaact
    24241 cgttgtcatg cctgccaaaa gctgaaaaaa caatcgacta tcagctacaa aaaaatgtag
    24301 acaaagaccg gcaaaaaata acattcctgt aattaaaata ccgtgtaaag gtacgccttt
    24361 cttagaaagc gcacaaaacc aatggggcaa acttttttct tttgctgttt gatataaaat
    24421 cctgctagta ctgtagactg cactatttgc tgaggacaaa gaagcaacca aaatcattag
    24481 attgacaatt gctgctccgc cttggcaatg gatcacttgc agcatagtca ctaaaggact
    24541 ttcttttaaa tctaaacaat gccatggaac aacaaataaa atcgacaaaa tcgttcctat
    24601 atagaaaaag agaatacgcc aaggcagaca gttgattgct tttttcaaat tcttttgcgg
    24661 atcacttgtc tcgccaacgg ttaagccgat catttcaatt ccagcaaaac tgaataaaac
    24721 cataggaaat gagactacca ttccagcaaa gccaaacggt agtaattcat cgatctgtcc
    24781 aaaattttct ttgattccga tagactgggt atgaaaatct gagacactca atcctatacc
    24841 caataagata aaaaggaaaa tccccaagat cttgatgatt gagaaaaaag attccaattc
    24901 accaaaccac ttgactgcaa caaggttgac tgccattaag atcattagtg cgctacctcc
    24961 gcttatcaaa gctggtacat gtacaaacca gaaatggata tacaatccta ttgctgttaa
    25021 ttccgcaagt ccagctccaa gccagcaaag ccaataagtc caattgacaa aaaatgccca
    25081 ctttttgcct aaataatagt caacgaaatc actgatagag ttaaatgtac tatttgctaa
    25141 aaataaatca cccatggccc gcatcaaacc ataaatagcc aaaccgcata tcaaataaac
    25201 gagaataaca gctggccctg ctgatgcaat tgcctttgct gaacctaaaa ataatcctgt
    25261 tccaattgcg ccacctaatg cgattaactg gatatttcga ttagttagag tccgtttcat
    25321 tgccggtttt tccatcaagt tcaccttttt ctttttcaag cagcatggcg acttcctgtt
    25381 cccaagaagg tttctttatt ttttcaatac agagtggtac tacaaacaaa atgacactga
    25441 tggctgtgac tgcaattaga ttgatccatg ttccagcttt catcgtcagt ccgacagaaa
    25501 atccgattgc cgcaaataaa acaataacga cagtccaaac tccaagattg ctctttttct
    25561 ctttttttcc aattctaaat ggtcgattca actttgcatg tgtataacgc aatctcaaaa
    25621 ctgcgatccc catgatgaca tagacgatac agtagataac agtagttgaa tttacgagca
    25681 tcaaaaatgc ttgattgacg ccaggaatca gcaaataaaa aagtgcaaag attgagataa
    25741 tgacagactg agtcaaaata acggatttgg atacgtcaaa ttgattcgtc ttccaaaaat
    25801 tgaattttgg tggatacgca ccttttctag cactttctgt cactgtcttt gctggtcctg
    25861 aggcccaagc agataattgg actgctacac caatgaagac aagtaaacta aacagattca
    25921 caataagatg tggcaaacca agaatttggc aataaatcga aatagattgc gcgatattat
    25981 tcaattccat ttgtcctttt ggtacgacat ttgctacaac taaagcattc aaaatattga
    26041 cgaaaaatat aaaaattaac gcggcaaata ctccgcgaat atacgtttta actggatttt
    26101 ccaatcgagt gatatacaca gaggacattt cgataccagt aaaaataaac atgatcggcg
    26161 caaaataaac aaatgaggat gcagtcgtag tatctggtat taatttgtcc catgaaaaac
    26221 tgcctaatgt tccggttggt acgatcccaa ctttgatcca cgcagcaaac cctaataaga
    26281 gcatcatcgt caaaggcacg tacaatccta accaaacacc gacttttccg ccgatttttg
    26341 ccatatcaaa tttcaaattc aaaaaagtga tgatccaata aacgactaaa atgacaacta
    26401 atgtaaattt gctgttcaaa ccaagcggca cattatcgat aatattacca aacaacggag
    26461 cgagtgctga agcgaccatc accatcccag gaaacatttg gacccataac agccatgaga
    26521 caacgaaccc ccattttttt cctagagcat tcgataccca aagttctgga ccaccttttt
    26581 ggggaaacat tccagcaaat tcaccagaga tcaatgagat tggaaaagcg tacaataata
    26641 ctgcaaccag catgtaaaaa atcatcgtcc agctcgttgc tgctacatct ggaatatttc
    26701 gtacacttgc tacaagggcg caggtcattc cgataaaggt catggtcgtg atttgctttg
    26761 atgaaccagt atctttcatc tttattcctt ctttcaatag cgacatatgt ttttgttttc
    26821 ttttattttt gttcaagaac agaaatgaca aggtttcatc gattcagtac aaaacccagt
    26881 atcaccgaaa catccattga acgatcttag tgaactgggt ttgatacatg aaacgaacga
    26941 tccaacttat agaacgtttt ataaaaaaga atatcttggc gaggaatata tgaaaaacag
    27001 ggggaaatac aattgttttg atgaagcaaa taattgtctg ttctccatat gtctcttgtt
    27061 attctttcac acaaaaatca gttctattgg atgatttcgt ttagtttctt ttccattgct
    27121 ttggcgattt tttttgtata aaaattgaaa attcgatcat cattcaaata aggagtcatg
    27181 atagccgcac gtagcagagt gattttctgt tcttttttcc attcttcttt cgttagaccc
    27241 attctttcaa caaaacgaat cggagaatca ccgtaatctt cttgtgtaaa tgtagtatga
    27301 gaagtaataa accgttcacc ataaacatcg ccatccatat aagaagaccg atcgaacatt
    27361 ttttcattta attcattgat agcgttcaaa tctgtacagc cttgttcttt gaatacccaa
    27421 ttgaccatat taaaatcagg atgattcaat ggatagactt cgattgtttt tcctttaaca
    27481 gtaaaagtca gatgatcgag aaattctctg aatctttgag ctgcctcgat tgaagcccca
    27541 atcaattgac catatccggt cacattcaat ggcaatacac gatgtgctgt ccagactgcg
    27601 gcggcggttg ctccagcttt tgatccttcc aaaatataag ctcctaacat atctggagcc
    27661 tttactgatt tttcaaacac atatggcgcg aaataagaaa tgatatttct catattttta
    27721 tgtttgatta cgattcctcc agcagcataa gggacatagc ccatcttatg aggatctatc
    27781 gtgacagaat ccgcttctga aatagctctg aacccttcat atacctcttt gtcaatcgtt
    27841 acgcagtggt ggaaaacatg atgttcttca aagaattcag ctaaggaagc atacggcaca
    27901 aattctcctg cttcgtttag aaacagtgaa cgcgcatagc cgccatatgc ggcatcaaca
    27961 tgtaaataga aatagatccc ttcatctttt aacctttctc ttaattggac gattttatct
    28021 acactgtcta cttgaccttc ttctgtcgtt cctacgacag caaccacacc tagaattggg
    28081 attttttgtc cagctaattc acgaattgtt ttttccaaag cattaatatc cattcgataa
    28141 tcctcttgaa ctgggatagc aaccatctga tctaagccga ctccgcaaat atctaacgcc
    28201 ttcatccaag aatagtgttt tgtttgaggg accagccatt tccctaatct ttggatattt
    28261 tttccgctac gggaagaagc tgctttgacc tcatccattt cttcgtccgt aaagctttcg
    28321 gtcatttcca aaatttcttc aacagataaa ttcaaaagtt cccactcaga catttttttc
    28381 actttttcag gcagcacttc cttgacagct aaaggaatcg atttgatgca acgggcgtac
    28441 cataatcctt caagattagc aatagaacca tctgctgcaa tgtgtcccca tccgtctgtc
    28501 atattgaata atgaagcaaa atcctgtcct acttctgctt ccatttgcga agttgccata
    28561 gaagattcca atgcaacatt atttgggttc cacagcatcg catagttgta agctaataaa
    28621 gcaggcatca atgtttcagc attcatttgt ccccagtaac ggcctgctga atgccatggg
    28681 atcgatcctg cgcgtaatcg ctgactgact tcatccaaga cctctatcat atggtcgcgg
    28741 gtggctagat aatctggagt gagcttatct ccttcgctaa ttgcaggcat atctgaagga
    28801 atataatttt ctctccatcc aagatgttca tctaccatct tgttcaacaa cattttataa
    28861 actggtccat tttcagcttt gtcgcctata aagacggcct tgatatccat atctttcatt
    28921 tttcttccct ccatattttg ttggtcaatg tcttgcagat acatgtgttt atctgtaaca
    28981 cctcttcact atagcgccaa aaatgagaat ttttttttta taacatcaaa tccacgtaga
    29041 tttttacaaa caaaaataaa taaagtcgga tttttctgat aattcaacgc aaaaaagcga
    29101 acaatttttc aataaatcag tcttctaaaa tagataacct aaaaaaagcg aacaaacaaa
    29161 taaaatgact gtccttcatt cactaaacat atttttattt ttttatattg ttaaaaattc
    29221 gcaatttatt ttttaataga attatctgtt ttccaaattt tataacataa aaaacctttt
    29281 ttaacggcat ttatatgctt tttcagaaat taactgatga tttcaaaaag aaaacatgtc
    29341 ataatatttc ccatattgtg caa
//
LOCUS       DANKKG010000021        28081 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000021 DANKKG010000000
VERSION     DANKKG010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28081
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.021"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            265..423
                     /locus_tag="PW004_000798"
     CDS             265..423
                     /locus_tag="PW004_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2725789.1"
                     /translation="MNKKQACEFLGIDNNTLDKWIRVYNFPVIKIDGVVRFDVEDIKD
                     FMNTFKKN"
     gene            495..1721
                     /locus_tag="PW004_000799"
     CDS             495..1721
                     /locus_tag="PW004_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2725790.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGIASEKYQKVFPTRQLAIKA
                     EREMKKKIETVLREENANSLELKGKIKFKDFYESKWLPRYELGQTTRSNRAPSNVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILCYIEFNRTTKIIQCITAPKKMALEEKRIREGNQALSSEELTDWIEAVNNDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFNKNTVRLKYALDKYQRKTHTKGWKDTV
                     IQAPEVVMTLLREWKSAQAEQLLKLKIIQTPDQYLFTYSKPSGEVNCPVHADYLNYRI
                     NAIKKRHPELAQLSPHKLRHTYATIAREGGANMNQISNALTHSDISTTKIYVNTPDVV
                     DKSVFEAFQKGLNKCD"
     gene            complement(1810..2202)
                     /gene="rpsI"
                     /locus_tag="PW004_000800"
     CDS             complement(1810..2202)
                     /gene="rpsI"
                     /locus_tag="PW004_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDL2725791.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(2218..2661)
                     /gene="rplM"
                     /locus_tag="PW004_000801"
     CDS             complement(2218..2661)
                     /gene="rplM"
                     /locus_tag="PW004_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HDL2725792.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(3180..3647)
                     /locus_tag="PW004_000802"
     CDS             complement(3180..3647)
                     /locus_tag="PW004_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HDL2725793.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(3767..5305)
                     /locus_tag="PW004_000803"
     CDS             complement(3767..5305)
                     /locus_tag="PW004_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HDL2725794.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            5385..6071
                     /locus_tag="PW004_000804"
     CDS             5385..6071
                     /locus_tag="PW004_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDL2725795.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(6272..6799)
                     /gene="msrA"
                     /locus_tag="PW004_000805"
     CDS             complement(6272..6799)
                     /gene="msrA"
                     /locus_tag="PW004_000805"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDL2725796.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(6883..7560)
                     /gene="pgmB"
                     /locus_tag="PW004_000806"
     CDS             complement(6883..7560)
                     /gene="pgmB"
                     /locus_tag="PW004_000806"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDL2725797.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(7557..8685)
                     /locus_tag="PW004_000807"
                     /pseudo
     CDS             complement(7557..8685)
                     /locus_tag="PW004_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
     gene            complement(8685..10868)
                     /locus_tag="PW004_000808"
     CDS             complement(8685..10868)
                     /locus_tag="PW004_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HDL2725798.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(10865..11890)
                     /locus_tag="PW004_000809"
     CDS             complement(10865..11890)
                     /locus_tag="PW004_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL2725799.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTANRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(11935..12762)
                     /locus_tag="PW004_000810"
     CDS             complement(11935..12762)
                     /locus_tag="PW004_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDL2725800.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(12759..13826)
                     /locus_tag="PW004_000811"
     CDS             complement(12759..13826)
                     /locus_tag="PW004_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HDL2725801.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(13852..14688)
                     /locus_tag="PW004_000812"
     CDS             complement(13852..14688)
                     /locus_tag="PW004_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2725802.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(14685..15554)
                     /locus_tag="PW004_000813"
     CDS             complement(14685..15554)
                     /locus_tag="PW004_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2725803.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(15624..16907)
                     /locus_tag="PW004_000814"
     CDS             complement(15624..16907)
                     /locus_tag="PW004_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDL2725804.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(16921..18606)
                     /locus_tag="PW004_000815"
     CDS             complement(16921..18606)
                     /locus_tag="PW004_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HDL2725805.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            18881..19906
                     /locus_tag="PW004_000816"
     CDS             18881..19906
                     /locus_tag="PW004_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2725806.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(20501..24175)
                     /gene="rpoC"
                     /locus_tag="PW004_000817"
     CDS             complement(20501..24175)
                     /gene="rpoC"
                     /locus_tag="PW004_000817"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HDL2725807.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVKETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(24233..27850)
                     /gene="rpoB"
                     /locus_tag="PW004_000818"
     CDS             complement(24233..27850)
                     /gene="rpoB"
                     /locus_tag="PW004_000818"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDL2725808.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLFALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
BASE COUNT         7837 a         6019 c         5212 g         9013 t
ORIGIN      
        1 ataattagat ataatttttg aataacgttc cctgctattc gagctagaaa tacaagtacg
       61 ccatgaccta gttagcgaca actacttaga gatttctttt gttctgggac aacaaaatgc
      121 catgacaata gcagtaaata atgatacttc cgtaaggctc gccgtaaact ggggagaggt
      181 gaaattccta tgtgcttacc caaagcggtt caaaaatttt aaactattat tttaggagat
      241 gattttataa aatcgaaagc ctacttgaat aaaaaacaag cctgtgaatt tctaggaatt
      301 gataataata ctttagataa gtggattcgt gtatataatt ttccagtaat aaaaattgat
      361 ggcgtagttc gctttgacgt agaggatatc aaagatttca tgaatacatt caaaaaaaat
      421 tgaaattttc ttctcgtcac atatactata aatagcgaaa tgtactgcct tactttttaa
      481 aggagtgagg aagtttgcct acaaagcttt caaatgggaa atataaaact aatctgaggt
      541 atccaaaaaa attcagagaa atcactggaa ttgcatcaga gaagtatcaa aaagtatttc
      601 caactaggca attagctatt aaggccgaaa gggaaatgaa aaagaagatt gaaacagttt
      661 taagagagga gaatgcaaac tcactagaat tgaaaggaaa aataaaattt aaagattttt
      721 atgaatcaaa atggcttcct agatatgaac taggtcaaac tactaggtca aacagggctc
      781 caagtaatgt aacaattagt aatactaagg atatattccg attgcacatc cttccaatgt
      841 ttggtgaata tgcaatgaat tatctgaatt caaatactga gataatatca gatgaattaa
      901 caaagaaatc gaaagaatac gcaaatataa aaattatcaa aggctatgtt cgctctatgt
      961 ttgatattgc cgaaatacta tgttatatcg aattcaaccg aactaccaaa attattcaat
     1021 gtattaccgc gcctaagaaa atggctttag aagaaaaaag gataagggaa gggaatcaag
     1081 cattatcatc agaagagctg actgattgga tagaagcagt aaacaatgac ttaaataatc
     1141 acttgcttac acttcacgat tacacacttt tcatgcttac cttatatcta ggtgatagaa
     1201 aaagtgagac ttatgctctg caatggaaat atattgattt taataaaaat acggttcgtc
     1261 tcaaatatgc tttagataaa tatcaaagaa agacacatac aaaaggttgg aaagacaccg
     1321 ttattcaggc accagaagtt gtgatgacat tattaagaga atggaaatca gctcaggctg
     1381 aacaattgtt gaaactaaaa attatacaaa cacctgacca gtacctcttt acctattcca
     1441 aaccttctgg agaagtaaat tgtcctgttc acgctgacta cttaaactat cgaatcaatg
     1501 caataaaaaa aaggcatcct gaattggctc aattatctcc acacaaactt cgtcatacct
     1561 atgcaactat agctagggaa ggtggagcaa atatgaatca aatctctaat gctttaacgc
     1621 actcggatat ttctactaca aaaatatacg tgaacacacc tgacgtagtt gataaatctg
     1681 tttttgaagc atttcaaaaa ggattgaata agtgtgatta acatgtgttc tcaaatgtgt
     1741 tcttattata aaaaaagaca ctttccaaat aattgaaaag tgtcctgaaa tattgatata
     1801 aaaagaatat taacgttttg agaattgtga agctttacga gcttttttaa gacctggttt
     1861 tttacgttcg accatacgtg cgtcacgagt aagtaagcca gcacgtttaa gagctaaacg
     1921 gaagtcagga tcaacttcta gcaatgcacg agcgatacca tgacggatag ctcctgattg
     1981 tccagcgtat cctccgccgt ttacgttaac aaatacgtca tatgcgcctt ttgtttcagt
     2041 aacagcgaaa ggttgattga ttacttcacg caagtcagca tgtggaatgt attcttcaac
     2101 atctttttta tttacagtaa ttttgccagt tcctggtact agacgtacgc gagcaactgc
     2161 gtttttacga cggcctgtgc cgatatattg aacttgtgcc aatgaaattc cctcctatta
     2221 gattaagttt gtgatgtcta aaacttctgg ttgttgtgct gcatgtggat gttctgctcc
     2281 accgtatacg ttcaattttg taaattgttt gcggcctaaa gtgtttttag gaagcatacc
     2341 ttttacagaa gtttcgatca aacgacgaga atttttagca cgcaattcgc cagcagtgat
     2401 tgatttcaat cctcctgggt agttgctgtg acggtaataa attttgtcag tcgctttttt
     2461 acctgttaat ttcactttgt ctgcattgat gacgatgacg aaatcgccag tatccaaatg
     2521 aggtgtgaaa gttggtttat ttttaccacg tagtacagat gcaacaactg ttgaaagacg
     2581 tcccaatgga acatcagttg cgtctactac ataccattta cgttctactt cgcctggttt
     2641 ggccatatat gttgtacgca cgttattttc ctccaattta agttctgatg tttgacatac
     2701 acttcgagtt tccggggctc tttgtggggc aaacaatacc atttaacata ttacccgcta
     2761 atctagtcaa tgtcaacgtt tttctgcttg attgaaaaat ttatatgaaa ctttacttct
     2821 tttctttctt ttatataaaa gggtcgtttc ctgtcaaaat gaaaaacaaa aagaggctgt
     2881 gacaaaagtc ttgtcacaac cccttatttt gaataaacgg tgttctcctg ccaactcctc
     2941 ggtcacaagt cacaatatcc ataaaacata agaagctgtt ttttaaaatt gtttcttgtg
     3001 actcggagct gaacggcagg cttacaacct cttggtaaac ggtgttcaat cagctgaccc
     3061 tcggtattag ctcaaaaaac ggcaaaatat gaggaactat tttgacgttt gtttctctaa
     3121 tactcaggtc aaaacgctga tttcacaacc tctttttcgg tcaagttcaa caacagtttt
     3181 tacgcgtcga tctctggcgc tttgcccaaa tgagcttgga tcatccagat acgtttttct
     3241 gtggctgttt tgaattcgat aaagatatct tgtgttggat cgtcgccttc ttctccagaa
     3301 acttcgattc ctttttggta aagatctgct agataacgat agcctgcaac aagtgtttcc
     3361 aaacgttctg gaatagtacg atcccagcgg ccaatttcat cagggatttt tgtgttatct
     3421 gcaaattcac gcaatgtaga ataaggtgaa ccgtctaatg tgatcaagcg ttcggatact
     3481 tcatctaact gagcgttcaa atcatccatg aactcatcca tcattgggtg caatgtaagg
     3541 aaacctggtc ctctcatgta ccaatgtgtt tggtgaacaa caacagacat ttggcttaga
     3601 tctgcaacta attgatttaa tacttctttg gtttgttgga atttcatctt tcttgcctcc
     3661 taacattttc ttacacttaa aataatagta gactttcaat gaaaagtaca acaaaatcat
     3721 ttacctgaga acgattttca gttcgctttt ttatccgtaa agccaatcat ttttcagaat
     3781 cagctaattc ttctcgcagt tgagcaaatt gctgataagt ctgtgctaaa tgcttttgtg
     3841 tcgaaacaaa taatggatag attttctgat attttttatg atcagtttct tgtactccaa
     3901 cttgctcttt caattctatc aattcctgtg cttcactgat actttggatc tttccgatgc
     3961 ttctccagcc taaaatcaca gctcctaatg cggatgcttc tgtgacagta gggaaaacaa
     4021 gcgggtaacc cagtacatca gccatcatct gacgaaaaat cggagattga gcaaacccgc
     4081 ctgtagcacg gatctctgtg agctgatttt cctggcaaac tccttttaaa atccgataaa
     4141 ggttgagaca gatcccttct agaatcgcct tcatcatttc tttttctgta tgttgccgct
     4201 gtaaaccgat aaaactgcca aacgcatctg cgtcccaaag cggtgctcgc tcaccaagta
     4261 aatagggatg aaaaatcaga ccattggcac ccgctggcac aaaagcgata gccgaaagca
     4321 gtgcatcata tggattttct cctttttctt tagcagattg aacgacttcc tgtaaaaatg
     4381 tatgccctgc ccaatcaaag atgccagcac cattgctagt cgctcctccc actacccaat
     4441 gtttctgatc cagaacataa caaaacgtct ccatttctgg atggaactga ggctgagtag
     4501 taataaaacg aagtgctccg ctcgttccta cggtcaaagc ggcgatcgta tcaccaaaag
     4561 cacctaaccc tagattagaa agcggcccgt ctgcccctcc caaaacaaat gatatatcat
     4621 ttggcaaacc tagttctgct gctgtttttg atggtagacc tgttacttga taggtggaag
     4681 gaaagacttg cggcaattgc tctattcgaa tgttcaagta gtcaagcact tcttctgccc
     4741 attggaattc atggatatca aaatatccag ttcctgaagc tgttccgtag tcgctgatca
     4801 gttcgccaaa caatctataa aagatatagt ctttgatacc gacaaaataa gcagtttttt
     4861 tgaataattc aggttggctg ttttgcagcc acttgatttt gttcaatggt gtcattggat
     4921 gaatcggcgt cccagtctgc cgataaaatt ctttgccaag cacggtatat ttcagttttt
     4981 ctgcttcgtc cgctgctcgg ttatctgccc aagtgatcat tcgagataac ggtgttttct
     5041 cttcatccaa caatatcagg ccatgcatcg cagaggagaa ggagacaaat tgaatgattg
     5101 tgtctgaagg caactgttta attacttctt ttataacggt gcagacacct tgaaagatca
     5161 attcctgatc ttgttctgcc atcccttgtg tttcttggat caaagggtag cgaaaataag
     5221 cggatgctgc ttcttgggct tcatctgtga aagcaactgc tttggtttgt gtggttccga
     5281 tatctaatcc gattgaaacg ttcatttttt cacctcaaat tttatgtatg tacaaaaatt
     5341 tagtttaagc ttgcgtatct tggttttgaa aagggggaga tggcgtgtta ctttatttta
     5401 tcattggttg ctgtatcgga tcatttttat gtttaaccgc ccagcggatt ccgttaggtc
     5461 actctattat ttatcctaga tctcattgtg tcaaatgctg tcgttcgctt tcttggtatg
     5521 aattgattcc gattctctcg atcatcgtcc aacgatttcg ctgccgctat tgcaggtgtc
     5581 ggttgccctt ctactatttg ttggcagaag ccttatgtgg tggtcttttt gcctggttct
     5641 ttactttttc ttccgcaaga aatttttcga cgttcatatg gatgctttcg gctttgctat
     5701 tttcgttgat ggatttattt tacttcatgg tccattctca cacgctattc tgttcttggg
     5761 caattttatg gattttttgg ttgcaggccg gcgtcttcca atggcaaagt gtgctgttaa
     5821 cgtttatcgt aggcggtgtg tgccttcgct atggtcaatc ctatctcgga gctggcgata
     5881 ctctgttgat gctttcttgg agtggtggct tgtctctaga agagttgctt cagatcttgt
     5941 ttcttgctag tctgtttgga atatccgttt tttctgttta ctttatctgt cataaaaaaa
     6001 agttggaaaa gctgcctttc attccttttt tgagcagtgc gttgctgatt gtccttcatc
     6061 ttaggtattg aaaaaaggtt gtgacaaacg acttgtcaca acctctgata tatagtgttc
     6121 tcttaccaaa tccttgtgac ttggagctag gcgacaggtt cacaacctct ccactaaatg
     6181 gtgttcaatc agctgaccct cagtattagc tcaaaatgct ggttttacaa cctctgatat
     6241 acggtgttcc ataagtagct ttttcggctt cttatttctt gacgccccaa tgtcgacgaa
     6301 ggaaacctgc acgtccagaa ccttctcgat aaagattgta atggagcttg tttttcttgt
     6361 aataatcctg atggtaatct tctgcaggat aaaattcgct tcttggttcg atttttgtca
     6421 cgattggctt atcgaatcgc ccgctgtctt gcaatgcttg ttttgaacgc tctgcaatat
     6481 ttcgttcttc ttcatcatta tagtagatca ctggacgata agaatcaccg cgatcagcga
     6541 actgtcctaa tgcgtcagtc ggatcggttt gctgccaata gatttccacc aactgatcat
     6601 aagaaataac ttctggatca tattcgatct ctactgcttc tgtatgccct gttgtgcctg
     6661 ttgttacttg ttcataagtt ggatttggta catgcccgcc agtataaccg gatgtcacag
     6721 aaatgatccc tggctgctct tcaaatggtt tgaccataca ccaaaagcat ccgcctgcga
     6781 aaactgcttt ttttgtcata taaaatcccc cttttttctc atcatacctt gatttccaaa
     6841 aggaggataa cgttttgctt gtgacagaaa aaacggaaac tattatatac gtgatacggc
     6901 ttctgataaa tgccaaaacg aacgtacttg atcaaaaacg gttaaatcag gaatcacata
     6961 ttcagcagaa ggaagattta tgttttcacc tattcccaat acatgcatgt taccagccaa
     7021 agctgcttga caaccagctt ctgaatcttc aatgaccaag catgcatttg gcggtacatt
     7081 caattgctgt gctcctttga gaaaaacttc tggatcaggt tttgcttttg atacttgcgt
     7141 gccgtcaata aggacatcaa aataacttgt caaattcgtt ttttctaaaa tgaggcgtgc
     7201 atttttgctt gctgatccca atccgatctt gatgtttgtt tttttcaagt actcaagaac
     7261 agctattgca ccaggcaaaa cggatgtttc atccattttt tgaatcgctt gaagatatag
     7321 attgtttttt tgctcagcaa aggctgattt ctctttttct gagaatttct gttccatttt
     7381 tccaaatgac agcaatcgct ctagtgactc ggttctggat attcctttca gttgctcgtt
     7441 ttctttctca gtgaatggaa tggacagctg gttagctaaa accagccacg cttcgtaatg
     7501 ataacgagct gtgtctacaa gaacgccatc taaatcgaat agtgctgctt tgattttcat
     7561 cgattacctc cttgaaaatg aacgacttga agtaaagatg cgatgttttt agctgtgcga
     7621 tgaagattat cagtggcttg ctgtaaagct tcttgtaaag gcacagctgc aggaaggatc
     7681 ggaaaaacgg ccgaaatgcc aacatctgct gctttttcgc ttcctttacc aacacttccg
     7741 caaatagcta tcacaggaac gccctctggt actcttctag caatcccaac tggcgctttt
     7801 ccttttagac tttgtccatc tagacgtcct tcgcctacga ttacgaggtc tgcttgctgt
     7861 acccgtatcg aactccagta aatccaatac gtagtcgatg ccagagacta ttttccctga
     7921 agcaaatgct aacagtcctg cacccattcc accaccagca ctactgcccg atgcctcgat
     7981 tacttgaggg gcaaatctca tataaaaatc atgcatggct tggtcagcca ctgctaattc
     8041 attttctttc aagccttttt gtggtccgaa aacaaaagaa gcgccttctt ttccgcataa
     8101 aagattagta acatcagtaa tgacagtgat tttggcttta atcagctcaa taggcacgtt
     8161 cgttttatca actgtagcaa ttttacctag attttctccc agtgcttcta ctttttgatt
     8221 atcttgatcg taaaaagcat aacctagacc ttctgccatg ccgatgccgc catcgttggt
     8281 actacttccg ccaacgccga tgatgatgtg tcgaacacct tgttgcaaca attgtcgtat
     8341 cagttctcct actcctcgcg tggataacct aagtggctgt tgttggttta gtgggacatc
     8401 tttcaatccg acgatttctg ccatttcaaa aatcgcagtt tgaccgtctg aagcgtactg
     8461 gatttctttg tttttgccaa atgcattagt aacgactgct gtttgccgct tcaaccttaa
     8521 actgtctgct aatgatgaaa ccgttccttc tcctccatcg cctataggta gcagatcata
     8581 ttcagcctga acaaaaactt cttgaaaacc tgattgaatg gcttgtgctg cctctaaggc
     8641 tgtcagactt tctttgaaag aatctggcgc aatcagaata cgcattattt tcccctccgt
     8701 tccaagatca gttctttttc cagctggtat tggtctccat tgacaaaaat aggtaatgtt
     8761 tctttcgtct ctttttttat tactatttgt ttttttgtca aaacaaatag caacctgtga
     8821 tttttccaaa taaaaggaaa agaaattttt tcccatgctg aaggaagtct tggagaaata
     8881 gaaagagttc cattcgttac tgtaatacct gaaaaaccaa atataactgc tagccatagt
     8941 gctcctaatg aagcagcatg aattccgtca tctgatgaat gcggatctgg tcctaaatca
     9001 atcaggcacg cttcttggaa taaatgatac gcccattcca tttcttggat acggcaagcc
     9061 tctatggcgt gaatcgcttt actcaacgat gagtcgtgga ttgttttgtt ttcatagtaa
     9121 tgcaaattct tagaaacgat tggttgagaa aaatactcag gtaataagta taacagcatg
     9181 acaagatctg cttgcttgct gacttggagt tcatttactt cgctacgggc ataatcaagt
     9241 aaaatagact ggctgccagg acgttctttg taagtggtca aatcaataac tggcttactg
     9301 agatacgtgt catcttgcgg aatcagctgt tctttgttag gcactggcaa atataactta
     9361 tctaaaaaat tttgacattc tttcaaaaaa agcggatcgc cagcttgata tttaacagct
     9421 aaggccacat tataatgcgc cagatagttc gtatatgcat tattgtcgat atattctgtg
     9481 tattcatctg gaccaatgac tctgtgtatc tctaaatgat catcgacttt tacagctctg
     9541 ctcagccaaa aagctgctgt ttcatgaatg agcgtttttc cctttgtttc taaaaacgat
     9601 tgatcctctg ttgcttggac atactcttga acggcataag caaggtctgc actgatatga
     9661 tgttctgcgg cagctgaagc aacttcttgt cttgttccgg ttttgatatt caatgcagca
     9721 aatttgggtg tctcttcctg accagtgtgt gcgctttccc atgggaaaag tgcaccttga
     9781 tagtgataat ctcttgcttt ttgccgtgcg ccttctaatc gatcataacg gtacagcagc
     9841 aaattctttg ctacttcagg aaactgatat aaaaagaaag gcaggataaa aatttccgta
     9901 tcccagaaaa catgcccttt ataaccttcg ccagttagtc ccttcgcccc tacactaaca
     9961 cttggatcat tgcggggagt cataatctgt aaatggtaag cagcgaagtc catcgctaac
    10021 tgatcgaaag aagaatcgcc ttggatcgtt atccgatgct gatgccaaaa atctgtccaa
    10081 taagacatcg actgctctaa aatttctgta taactaacgc ttttctgggc ttgggcaata
    10141 cttttcttaa acgggtctgt ttgctgaaga gaggtagaga gcacgatgat cttctccagc
    10201 gtaaaggacg tattttttct gatcttttct gaaaaggaag cggtgatccg gcgatttttt
    10261 gcttggaata cacctgcttt attgaattta gctgcaatcc caatatcaat tttactttct
    10321 gttgtctggt aggatgcact gatcaaatcg tcaccaaata ctcgcagttc tttttcaacc
    10381 aactgctgtg taccaaaatt ggtttgctgt ccatcgatac ccgttttcac atctacttgg
    10441 cagtcttcgg tcaacggctt gatgaccact ttcagggctg cctgatggag attattttga
    10501 tcagcaacac gacaagtaat gacttccact ttttttcctt gagcagtttc aatgagcgca
    10561 tagcgtttaa gctctcccat ttctaaatct agtgcccgct cgtatgcaag aactttcatc
    10621 gtctcaagag aaaaatgttc accgtcgatt tttatttccc atctgatgac atctgggaga
    10681 ttcactaact cggctgattc tgctcctaaa ggtcggttat atacaccagc tgcaaacatt
    10741 ccccttactt gattccgata gtcttcctcc tggcttcctc tgataccaaa aaatccattt
    10801 ccaatggtca tctgactcgc atacttattg atatactcta aatcaaactg tttttcttct
    10861 actttcataa ggtaataact tctcctgttt ctgccgaacg atataaattt tccaccagat
    10921 tctggatgac atatccttgc cagccatctg caatcatctg agtctggtca ccgagcgccc
    10981 gattcacaaa atgagccata cttagttcat gtgggtcatt aactggcgtg gtcttttgta
    11041 aggaaaccag ctttccatct acatcttcga atagttcgcc tggatacaga ttagcacctg
    11101 cctgatcgcc acaaaaagcc acattcagtt tgtcttctct gatattcaaa gcaaacgaag
    11161 tattcagttg gatgatcttg ccgttttcta attccagtgt gccaaaaatt gcatcttcta
    11221 ctgtgaattt gcttggatcc catttcccga atttccctga attttttttc gtaccaattt
    11281 tttggaagct gtacgcccga acgcttttta ctttaggaaa tccagtaata tacatcgctg
    11341 tatcaagcat gtgaatgcca taatcgatca aaggcccgcc tccttgaagt tctttgtcga
    11401 taaacgttcc ccagcctgga acaccagacc gacgaagcgc attcgcttct acgtaataaa
    11461 tatccccgag aacatcaaga tgattcttga tcattgttgc ttcgtcagaa aaacgatgct
    11521 ggaatccata agccaaaatc tgcccgtttt tatcggctgt ctctgccatc tttcttgctt
    11581 gatcagctgt catagctggt ggtttctcgc acatcactga agcattagct tcgagggcag
    11641 ccaatacatg ttcgaagtga aagcgattgg ctgtacagac actcacgata tcgggagaaa
    11701 tctcattcag catttgtgta actgatgtat acgcatgaga aaatcctgta cgtcggcaaa
    11761 agtcttctgc tcgctggctg actggatcaa ccacagcggc caattctaag acatctgatc
    11821 ttttttgata ataagcagca tgtacttttt ctgctacttg tccagcgcct atgattgcta
    11881 ctttcttcat ttatttctct ctcctttgct tatttactta ttgttattct ttctctattc
    11941 tttctctaaa tcatttaaat gacgcgctgt ttccagacag tttttcaagt attgcaatga
    12001 ttcctgataa attttcactt cattttctcc tcgcaaacga cattcatata ccatataacc
    12061 ttcatactca ttttgaagta gcgtttgaaa atggcgagta aaatcaattg cgccagatcc
    12121 tggttggtag cgatgattat ctgctaaatg gatatgaccc acatactctt tttgcgtagt
    12181 caatgctttt gaaatatcat cttcttcaat attcatatga taaaaatcag cgatgatctg
    12241 cacatgtttc aattgattag agtcaatgta atgacgcgca tccgctaaca aattgatcat
    12301 atggtcttgg tatcggttca aaggctccaa ataaataacc gtacctgttt cttcggctac
    12361 tttatctaaa taacgaagtg attcgctgac tgctttatag tctccttctt tgcttcgggg
    12421 tgaaaccatc ggcggtaatc gataagtgaa cattccccaa gctgctggga taacgattcc
    12481 tttgcctcca acctcgctta gtgcacgcaa aatctcagca atttgacgta caccattaag
    12541 acgccgttct tcgataaagt caccgatcca cccatcgtag ccgttacaag ctgtcactac
    12601 tgggatccct gtttctttga ttgcctgttt gactgtgtcg atattttcta ttaacaactt
    12661 accgtcgatt tcgtattctt caaatccaca gtctttgata aatctaaatt tttctaaaat
    12721 gtctcttggg aaaaatgctt gatcttgcgt accgaatttc attgttctcc tcctcggact
    12781 tgaacgccca ttttgatact ttcttctggg tgctggtcta cataacgcat gtaagcttct
    12841 gcgctttctg caaatggcac aactggatca atgatttctt cacaatccaa gtaaccgttc
    12901 atcaataatt cccaacatgt gtcctcgatt cgtttacggt tccatcttgg gtaatctggg
    12961 tttggttcac tagatgctcg agcaaagacg atttttgcat tattgaaatg tgcttcgcga
    13021 ccaagattaa acccagctgg aaaaggttta gcaaaggcaa cataagaaat gatcccgcca
    13081 tagccgatcc ctcttaaaga agcttgtaat gcggcagcac tgccactcgt ttcaataact
    13141 gaatctactc cacgtttatc tgtcaattcc tttagacgaa aaccgacatc tgctttcata
    13201 ggatctagcg tgtaatcggc accgtatttt tctgctaatt ctctacgctg cgctatggga
    13261 tcgatcccaa cgatggtagt agcacctgct tttttagcta attgaatcgc aatttgcccg
    13321 atagcgccta aaccgaagat tgctacagca tcacctgctc gaacatgtgc ttcccggata
    13381 ccacctaaag cgaattgagc aggatcgtaa catactgcat ttttccattg atccaaccga
    13441 tcaagtttac gtagtttata attatcaaca ccgtttgtaa tcagcgtttc acgaatgggt
    13501 ccataagtgc agactaactc tcctttttga tactctgtca ccaaactgcc cgtttcttcg
    13561 atcgtaccga caatcatatt tcctaattga aattcgccaa ataccacacc gcgttggctt
    13621 ccttcttctc ttggcaagaa taggcgccat tcttctgaaa attcttcatc gataaatgga
    13681 cttaatcctc taaagtcgac aatttctgtt cctggttttg gagtagcgaa aattactcgg
    13741 atacgtactt cgttcttttc gatctcacgg tcttcgtaat ctttgaatcc ggctattctt
    13801 ggttctaatg caactaattt tttcatcttt tctttcctct ttcttttctt tttatccttt
    13861 tacgccgcct tcgactaaat cacctttaat aaatcgttca gataacgcat acataatgat
    13921 gactggtaaa gcggtcacca atgaagcggc catcattcgt ccccaaatgt agtctggtgt
    13981 actgaataat gtgttcaaac cgattggtaa agtaaatttt tcggaggtcg acaggaaaat
    14041 cgaagcaaat aaataatcat tccacgaaac cataaagcaa taaacgaaga cagaaatgat
    14101 tccagaaata gccaatggta taatgatccg aataatgatc tggatccggc taagaccatc
    14161 gatcattgct gcttcttcaa tatcagtcgg aatcgtgtca aaataacttt ttagcatgaa
    14221 tatcgcagta ggaagtgttt gaacgaccat cgtgataatc agtgctgtat tggtatcata
    14281 caaacctaga ccggaaatga ttttaaataa tggaacaacg atcaaaatac cggaaaacat
    14341 ataaacggtg taaaagctag cattgatttt cgagcgagct ttgaagcgta gttttgacaa
    14401 agcgtatgcc ccgctgatcc caatcactac tgcagttacg gaggctatca aggagatcac
    14461 taaactattt ttgaaataca tgatgaatgg aaaaatctca gcattgaaaa tatcctgata
    14521 atgttcaaaa gtccattctt tcggaaaaat agtttgtgtc gttgcaattg cttcttgtga
    14581 atccttgaat gatgtcatca gcatgatcaa aaatggaaac aaattgacag ctaatagcaa
    14641 caaaatcgtc gcgtaaaata agattttcct gctcttttta tttttcattt caagtccacc
    14701 ttctttcgtg agagcaagac gaccgctgca ataatgatga acaatagaat cgatatagct
    14761 gctgcttttc caagatcatt gaacgcaaag gctgtctgat acaaataaac gcctaagata
    14821 ttgactttat tcgtcagtag atagacatct gtaaacatat agaacatcca gatagatcgt
    14881 aaaacaatca cagttgctag tactggtttg attgccggca gtgtcacgat cttgaacttc
    14941 tgccaagcat ttgcaccgtc catttcagct gcttcataaa gcgttttgtc gactgtttgc
    15001 aaaatagcca aaaatgaaat aaacgcatac ggatagtatc tccagatagc aaacaaaacg
    15061 accataaaga agctagcaac tggctggtcg aaccacagcg gtgcttcgtt gaaaagctgc
    15121 aacctatcta ctaacagatc attgacgatt ccataactat tattgaacat atatttccaa
    15181 gcaaaaatca aagaaataga tggcgtaaca taacttaata aaatcagtga acgcaccgtt
    15241 ttcctaaatc tgaacttgcg gttaagcaaa atagctacgg ctaatcctaa agctgtactg
    15301 cctgctacaa ctaagaatgt gtaagcaact gttaaaccta aagaacgata aaattcacta
    15361 tcacttaaaa tttcaagata attgcttaat ccgataaaaa cggcgtctga gttaggattc
    15421 agcggcatat cgaaaaaact gatctgaata ttggaaagca ttggaatcag cactaaaaca
    15481 gctaataaaa gcatgcttgg tgctaaaagt cccatggcta attttaagtc agaatgtttc
    15541 cttggacgat tcattgaaac ctccccctga taatctgcca atagctatct acttttttcg
    15601 aacaccattg gcagaacttc ttgttatttc gtattttttt gtgcgtcagc taaagcttga
    15661 tcgacatctt cgtttccgac agttacttgg ttcactgctt ttccgatcac acctgagctt
    15721 gtgatatctc ccattttcgt gaaattcttt tccccgacta gtccaaagac ttgtacttca
    15781 tcaaaagcac tagcaatctc agatggcagc tcgccaaaag acttgatgac ttcattttct
    15841 ttgtaaccat ccagttcgac tacttgttta ttcactggtt gagcaccacc aggagacatc
    15901 aagacccact tttccatgtt tttcggttga gataagaaag ctgtgaatgc ttcagcagcc
    15961 tctttttgtt tctcgtctag accagaagaa atcgtcaatc cagatactgt gccatagaca
    16021 gcttgatctt tttgtgttgg gatagcaaaa ccgatatttt ctgagttccc cgcttcataa
    16081 acagaaggca aaatgtaagt tgaatagatt gccattggtg ctgttccatt catgaaagca
    16141 tctttgattt ctgtcacatc attagagcct ggcatcgtgt attgggataa gttttgataa
    16201 aacgacaatg cttctctcat cttttccgta tcgatcgtta cctcgccttc atcatccaaa
    16261 acatttgctc catttgacaa agcaaattga gagaacgctt gctcactcat tgttccttca
    16321 gcagtaggca tagcaattcc atattgttta ttctctttat ctgtgaaatg ttgagcaatt
    16381 tttaaaatat catcccaatt ttctggttct gaaaaaccag cttctgataa tttttctttg
    16441 tcataccaaa taccttgaac ccagccgcta attggtgcag caatatagct ttttccatct
    16501 tctgaacgaa caaggttttt ggctccgtca taataattat cttctccagc ttcctcgata
    16561 acgttttgga ctgccgtctg atcaatcagt tcatctttat ccatcacttt agcaaaatcc
    16621 tgactgactt ccattacttc tggtaattta ccggaacgag ctaacgtaac gatttttgta
    16681 ttgaacgctg attcttctac cggcacttgc ttgattttga tgtctggatt ttctttttca
    16741 aaatcagcaa ccagttcatt gatcacactt aatcgttcct gttcgacaga agagtgcatg
    16801 aattgaatcg ttgtctgttg tgattcgtta gagctttgac tttcaccaca agcagtaaga
    16861 agaaatccag tagctagcag acaggtagta acaataattt ttttcatttt tttccacctc
    16921 ttattttatt attttcagcc acatatattc atacggctgg aatatcaact ttcgttcttg
    16981 actgcaatgg ataatttgtc cagaaaacag gtctaggtaa tctccttttg gtaaaagata
    17041 acctgctgat tgattggaaa aattatggat aactaagact ttctcttcct tcgcttcgcg
    17101 aataaaactg ataaaagaat ctgctgagtc cagtaagtaa atcaaactat tcggatggaa
    17161 ggctttttct ttttttcgaa tagatagcat ctgggtcaat tcaccatata ctgctgttct
    17221 taatggatct gtctgcaact ctttctcaat atctgctaac aaaaattttt gccgattgat
    17281 ttttcgattc tctcctgttt tttctacgcc tttgtaatca tttcgacttc ccagcacact
    17341 ttgaatataa attgccggca cgcctggtaa cgttaccaaa aatgaatgag cagctaaaaa
    17401 gcgtttgctt ctccattccg tcgtatctgt tggagaactt aacgcatcta gatacgtagc
    17461 gtttatctca tatggactgg tcgttccatc tgggtttttc ttgtagttta ctcgggcacc
    17521 ctctttcttc agctgttcca ctaattcaag gatcgttttt tccggcagaa tacctcgtac
    17581 cggattcaat ccgataccgt catgggaggc taaaaaatta aaaaaagttg tttttttcga
    17641 tgcgaaatcg atggttttta accattcttt caacttactt gtattacccg aacgaatcgc
    17701 ttgaagggtc aaaggtggta gcgaaaactg atagaccatc tgtgcttcat tctcgccatt
    17761 cccaaaataa ctgatattat ctttgtgtgg cacatttgtc tcagtgatca ataccgttcc
    17821 tggagctgct tcatcagcga ttgcacggaa taattggatg attttatgcg tttctggcag
    17881 gtgtatacaa gaagttccta gttctttcca taaaaatccc acagcgtcca agcgaataaa
    17941 tgaagcgcct ttttctaagt aaaacaaaag aatatcgatc attttcaata aaacttctgg
    18001 atttttaaaa ttcaagtcta cttgatcttc gctaaaagtc gtccatacgt gtttttcttt
    18061 cccgtttttg tcaatgaacg ttgtaagtaa cgggctggtc cttggtctag tcacgctgct
    18121 tgtatctgta tccagtggca aatcaataaa aaaatctttg tacgtatcat tcaagtttaa
    18181 atattcctga aaccaagtac tttcggcaga catatgattg cagacaaagt cgaacattaa
    18241 ccgtgtttcg cttctcattt cttctatatc ttcccatgtg cccaaacggt cgttgattgc
    18301 ctgataattt acaacggaga aaccatcatc agatgtatat gggaaaaatg gcaagaaatg
    18361 tacaactcta aatgtatctg ccaaatactt ttgataaaat ttcttaaatg tacgcaaagt
    18421 tggttgctgc tcttcaaaaa attgatcccc ataagtaatc aagaaaatat cttcttcgtc
    18481 ccaatgcagt ttgcctgcct ggcctttctt ctgctgataa acactgactt tttgttcaat
    18541 catagaggcg gttttctcag acaaatctga gtcgcgataa atcgcttgta aaagttttgt
    18601 atacatcgga ttcccctttc ttttttggga gcgttcccaa cgatttgatc atattatgga
    18661 aaacgcttac tgtcaatagc atttttttaa ataattttga tatacacaaa aatacaaata
    18721 agaaagctta tgtatcaata attaatagct tcttaaaaaa atatggtctc tcatgatcat
    18781 gcaagctatt cctaaatttt caaaatcata gacaaaaaag aatgtctatt gtactatgga
    18841 attattctaa aacgctccca aaaaggaggt gcatataaaa ttgaaaccta ctatttatga
    18901 cattgcgaaa aaagctaatg tctcaaaatc aactgtctcc agagtattaa ataaccagcc
    18961 aaatatctct gataaagcaa aagcaagtgt acttcaagca atcaaagaat tgaattatga
    19021 accgagcaaa attgcaagag gactgtcagg aaatggtttt gacgcgattc tagcattatc
    19081 tcatcgtact tctcatacga cctatggcaa tccttttttt tcagatgtta ttcaatccat
    19141 ttctgccgtt tctgaacaaa ataacttcga cttgattcta caaacggcaa aaacaccaga
    19201 agaagagcta gaaaaatgcg cgaaaaagat ccaagaaaaa atgatccgag gaattattat
    19261 tttaagctct acgatggatg aaacacttct ttttgaattg gatcaataca atattcctat
    19321 tgtggtaatc ggaaaagtcg gaaacgacta cacgaacatc tactcagtgg acacggataa
    19381 tttccaggat agttacgatt tggtggaaac actgatcaag aatggacacc gtcatatcgg
    19441 ctgtctccat gctccgacag aagtttatgt atccaatgat cgtctcaatg gttaccgtca
    19501 atgcttattt gatcaccacc tagatatccg taatgaatgg attatagacg gcggctttcg
    19561 cttagaagat agcatggcag caatgaatga tttaatgaag ctacgagaaa gaccaactgc
    19621 gattttttct accgatgcat taaaatcatt gagtatttac aaagtggcag aacaatctgg
    19681 aaagcagata cctgatgatc tttcgatcgc aggattcagt gatcccttat tgtcctccct
    19741 attttcgcca cagttgactc ggatagacat tccgacaaat cagttaggaa tcaaagccac
    19801 agaactttta ttccaactaa tccaccacca ataccctgaa gaaaaaagaa tcattctttc
    19861 aaccgagatt actctcacag attcgatcca acaacaagaa atgtagaaca gaattataaa
    19921 agaacagaaa cactgaggat aagtcagtta ctctgttctt ttttcaatca ctcatccctc
    19981 gttaccccct cccggtattt tagcgaattt ttcttatttt ccatctgttt tagatagctg
    20041 tggataacta taatgtttgt gttaaactat tttttccaca gggaagaaaa aactattttt
    20101 aaccacgttg tgtataagtt tttatagttc gtttttttaa acacatttac ccttatttca
    20161 atccatgtta tttggggata cttatctcta aaacataaat agaataacag acatgtctac
    20221 tggttttgtt ttattcgagg aattattcaa taatagctag caaaatatta aaaataaccg
    20281 ctctatcttc ccatcaattt attttgcatt caaaaaatga tctatcttta cttttgttga
    20341 ttgacttctc ttggaagatg atcgttccga taccttaatt ggatacgaac cgatgttgtt
    20401 aagatccttt cccacttttc ttctagttag acaaattatt ttagacaaac caaaaaagag
    20461 gttgtgacaa taatttgtca caacctcttt tttaaaatat ctattttgtt tgattgttca
    20521 ttgcgtcttc tgcagccatt tgcgcttcaa tatctgaaat actgtagaca ttttcgcttg
    20581 ctacaccgac ttcttttggt tccatgtcac gataacgttg cataccagta ccagctggga
    20641 tgatcttacc tataataaca ttttctttca aaccaagaag aggatctttc ttaccgcgga
    20701 tcgcagcatc cgtcaagaca cgagttgttt cttggaatga tgcagcagat aagaaactat
    20761 ttgtttctaa tgatgctttt gtgataccta gtaagacagg tcgagaagtt gcaggaacgc
    20821 ctcctgagac tagcgtattg tagttttgat ctttaaagtc tgcgatgtct aataatgtac
    20881 ctggtaagat atctgtatca cctggatcca ttacacgaac tttacgcagc atttgacgaa
    20941 ccattacttc gatatgcttg tcgccgattt ctaccccttg catacggtaa acacgttgta
    21001 cttcacgaag caagtagttt tcaacagata atacatcgcg aacttgcaac aattgttttg
    21061 gatcgattga tccttctgtc aatggtgcac cacggtggat catatcgcct tctgctactt
    21121 tcatacgtgc tgtataagga acagtatacg tacgtgtatc tgtttttcct ttgattgtta
    21181 cttctttggt acgtgttgca ggatcttctg agatatcgat gacatcccct gttacttctg
    21241 tgatcactgc ttgccctttc ggattacgtg cttcaaagat ttcttggaca cgaggaagac
    21301 cttgagtgat atcgtctccg gcaaccccac ctgtatggaa tgtacgcatc gtcaactgag
    21361 taccaggttc accgattgat tgagcggcga tcgtaccaac tgcttctccg acttcaacgt
    21421 cagagccagt tgcaaggttg cgtccgtaac aatgtttgca gacaccatgt tttgtattac
    21481 atgtgaagac agaacggatt gtcactgttt cgacacctgc atcaacgatt tgttttgcga
    21541 tgtcttcgga aatcaattgg tttcctgcaa tgatcaattc atttgtttca ggatgacgta
    21601 cttctttacg ggtgtaacgt cccactaaac gatcttctaa tgattcgatg atttcatttc
    21661 cctcgcggat ggcttggatt tcaagacctc ggtcagtacc gcaatcttct tcacggatga
    21721 ttacatcttg ggccacgtca accagacgac gagtcaagta acctgaatcg gcagtcttca
    21781 aggctgtatc ggtcatccct ttacgagccc cgtgagttga gataaacatt tccaagacgg
    21841 atagtccttc acggaagttc gaaatgattg gcaattccat gattcgacca ttaggagcgg
    21901 ccatcaaacc acgcattccg gcaagctgtg taaagttgga aatgttacca cgagctccag
    21961 aatcagacat catgaagatt ggatttttcg cttccaaacc ttccatcagt ttttgttgga
    22021 tgctgtcttt tgcagcattc caaacagcaa tgacacgttc ataacgttcg tcatctgtga
    22081 tcagaccacg acggaattgt tttgtaatag tttcaacttg tttatgggca ttttcaatga
    22141 tttcttgttt ttcattcagt acactgatgt cagcgatccc tactgtgata ccggcatgtg
    22201 tcgaatgttt gtaccctaag tctttcatac ggtcaagcat cttagaagtt tcttttactt
    22261 tgaatcgttt gaagacttct gcgatgatgt ttcctaagtt tttcttcttg aacggtaata
    22321 ctaattcttg ctctttgata tgtgcaggaa tatcagtacc agcttcaaca aagtatttgt
    22381 ccggtgtttg gacggtcaag ttgaagtctg ttggttcatt cagataaggg aattcaggag
    22441 gcatgatctc gttgaagatg atcttaccaa ctgttgtgat catcgtacgt tctttttgcc
    22501 attcagtaaa tggtttttca cctaatgagt tagggttcac accgatacgt gaatgtaaat
    22561 gaacatatcc gttgcgccat gcgataacgg cttcgttttg atctctgaag accattcctt
    22621 cgccttcgcg tccttcttct tccattgtca agtagtagtt acctagaacc atatcttgag
    22681 aaggagtaac aactggttta ccatctttag ggttcaagat gttttgagca gctaacatca
    22741 acatacgtgc ttctgcttgt gcttcttcat tcaatggaac gtggacagcc atttggtctc
    22801 catcgaaatc ggcattataa gcttcacata caagtggatg caaacggatt gcgcgtcctt
    22861 gtactaaaac tggttcgaat gcttggatac caagtcggtg aagcgtaggt gcccggttca
    22921 gcaatactgg gtgttccttg attacgtctt ctaaaacatc ccatacttcg tcttcttgac
    22981 gttcgatttt acgtttcgca tttttgatgt ttgaagctag ttcgcgttga acaagctcac
    23041 gcatcacgaa tggtttgaat aattcaatgg ccatttcttt tggaagtcca cattggtaca
    23101 tcttcaagaa aggtccaaca acgataacgg aacgacctga gtagtcgaca cgtttaccta
    23161 gcaagttttg acggaaacga ccttgtttcc ctttcaacat atgagaaaga gatttcaatg
    23221 ggcggttacc tggtccagta actggacgtc cgcgacgacc gttatcgatc aatgcatcta
    23281 ctgcttcttg cagcatacgt ttttcatttt gaacgatgat tccaggtgca ttcaagtcta
    23341 acaaacgttt caaacggtta ttacggttga ttacacgacg gtaaagatca ttcaagtcac
    23401 ttgttgcaaa acgtccacct tctaattgaa ccattgggcg taaatctggt ggaataacag
    23461 ggataacgtc cataaccatc cagcttggtt gatttcctga agcgcggaat gcttctaaaa
    23521 tatccaaacg gcggattgca cgtgtacgtt tttgtccttg tgcagatttc aattcttctt
    23581 tcaattcggc tgcttctgca tctagatcaa cattatcaag taattgttta atcgcttctg
    23641 cacccatagc agcttggaat tcttgtccat attgttcacg tttttcgcga tattcgcgtt
    23701 ctgtcaacaa ttgttttttc tctaaattcg tatttcctgg ttcgatgact acatatgaag
    23761 caaaataaat gatttcttcc aaagcgcgtg ggctcatgtc taatacaaga cccatacgtg
    23821 aaggaatccc tttaaagtac cagatatgtg aaacaggagc tgctaattca atatgtccca
    23881 tgcgttcacg gcgaactttc gaacgagtaa cttccactcc gcatcgatca caaacgattc
    23941 ctttataacg gatacgtttg tattttccgc aggcacattc ccagtctttt gtaggaccaa
    24001 aaatccgctc acagaacaac ccttcgcgtt caggttttaa ggtacgatag ttaatcgtct
    24061 caggtttttt tacttcaccg taagaccagc ttctgatttt ttcgggagaa gccaaaccta
    24121 tttgcatact ttcgaattta tttacatcga tcaaaagggg ttccctccat ttcatttaat
    24181 ggcaaaccgc aagacggctg atcttcgacc gccttgcagc tcatagaccg gattagtcta
    24241 aattatcttc tgcggtttca aagttttgga tgacatcgtc tttttcatct tccacttgtt
    24301 cagcagcttt cttttctaat tctttggcag tttgctgttc ggcaaatttt gtcaaagcat
    24361 caacagtgat caaatcgtcg tcttcatcgt ccatgtcgcg aagttcgatc tctgtttctt
    24421 cgatatccaa gacacgcatg tccagaccta gtgattgcaa ttctttcacc aatacgcgga
    24481 atgattccgg tacacctggt tttggaattg gttcaccctt gacgattgct tcgtatgttt
    24541 tcacacgtcc gactacgtca tctgatttgt atgtcaagat ttcttgtaaa gtataagccg
    24601 caccgtaagc ttccagtgcc catacttcca tttcaccaaa acgttgtcca ccaaattgag
    24661 ctttacctcc caatggttgt tgtgtaacca atgagtaagg tccgattgaa cgtgcatgca
    24721 atttgtcatc gaccatgtga gcaagtttga tcatgtacat cacaccgaca gacacacgtc
    24781 catcaaatgg ttcacctgta cgtccgtcgt acagaacagt tttcgcatcg ctggccatac
    24841 cggcttcacg aactgtttcc catacgtctt catcacttgc accatcaaat actggcgttg
    24901 ctacgtggat acctaactga cgtgcagcca tacccaagtg taattccaat acttgcccga
    24961 tgttcatacg tgaaggcacc cctaaaggat tcagcatgat atcgattggt gttccatcag
    25021 gtaggaatgg catatcttct tccggcataa tacgggatac aaccccttta tttccgtgac
    25081 gaccggccat tttatcccct tcatggattt tacgtttttg aacgatataa acacgaacta
    25141 acatgtttac gcctggtgac aattcatctc cagcttcacg agtaaagatt ttcacatcgt
    25201 gaacgatacc gccgccgcca tgtggtacac gtaatgatgt atcgcggact tcacgagctt
    25261 tttcgccaaa gatcgcatgc aatagacgtt cttctgcaga taattctgtt accccttttg
    25321 gcgtgacttt ccctacaagc aaatcgccat cttggacttc tgcaccaata cggatgatac
    25381 ccatttcgtc taaatctttt aatgcgtctt ccccgacatt agggatttcg cgtgtgattt
    25441 cttcaggtcc taattttgta tcgcgggctt ctgattcata ttcttcaata tggatagaag
    25501 tgtatacgtc atcttttacc aaacgacggc tcatgatgat cgcatcttca tagttgtacc
    25561 cttcccaagt catgaaacca actaaaacgt tttggcctag agccatttcg ccttgttcca
    25621 tagatggtcc gtctgccaga gtatcccctt tttcgacttt ctcacccaaa tgaacgattg
    25681 gacgttggtt gtagcttgtt ccggagtttg aacgacggaa ttttgttaca tcgtatttat
    25741 ctaatgcacc gttgtcgcgg cgtacgcgga tttcagaagc atcaacgtat tcaacgacac
    25801 catcatgttt acataacaaa gcagctcctg agtcatgcgc tgatttgtat tccataccag
    25861 taccaaccca tggtgcttga ggattgatca acggcactgc ttgacgctgc atgttggcac
    25921 ccatcaaggc acggttggag tcatcgtttt ccaagaaagg aatacatgct gtcgcaacgg
    25981 caactacttg tttaggtgat acgtccatgt agtctacttt atcaatagat acttctaagt
    26041 tttcactttg agcacgagcc ataacaacgt cttgagcaaa tgttccatct tcattcaatg
    26101 ggctgtttgc ttgggctacg atgtaatggt cttcgatatc agctgtcaag tagtcgattt
    26161 ggtcagtaac acgacctgtt tcacggtcta cacgacgata aggtgtttcg ataaatccga
    26221 atttatttac tttcgcataa ctggacaaac tattgatcaa cccgatgttt gggccttcag
    26281 gagtttcgat cggacacata cggccatagt gggagtagtg aacgtcacga acttcataac
    26341 cggcgcggtc acgagtcaat ccgccaggtc ctaaggcgaa aagacgacgt ttgtgtgtca
    26401 attcgccaag agggtttgtt tgatccatga actgtgacaa ctgagaagaa ccaaagaatt
    26461 ctttgatact tgccacaact ggacggatat tgatcaattg ttgaggtgtc aatgtttcag
    26521 tatcttggat cgacattctt tcacgaacca cacgttccat acgtgctagc ccgatacgga
    26581 attggttttg taaaagttca ccgactgaac ggatacgacg atttcctaag tggtcgatat
    26641 catcgacatt gccgatacct tccatcaagt tcaagaaata gctcattgaa gcaatgatgt
    26701 cggctggacg aaccgttttt acagctgctt ctggatagcc gtttccgatc acgtttactt
    26761 cacgttcagg gtctcttggt gaaaatactt tgatgacttg gacagtcatt ggatctgtga
    26821 ccacaccgtc ttctgatgga tagtaagtca ctgaatttag gccattatca atgaaaggag
    26881 ctagtgtttc catgacttgg tgagtcagaa ctgttccttt ttcaacgatg atctcgcctg
    26941 tttctggatc aactaatgtt tcagctaatg tcagattcaa tagacgtgtt ttcaaatcta
    27001 attttttgtt tactttgtaa cgaccaacgt ttgctaagtc gtaacgtttt ggatcgaaga
    27061 aacgagcatt cagcaagtta cgtgagctgt ctgctgtttt tggttcgcct ggacgcaaac
    27121 gttcgtaaac gtctttcaaa ccttcttctg ttcttgagtc gcttgcgttt ttatgcaagt
    27181 ctttttcgac tgtattgcgt aatgtttcgc tgtcgccgaa gatttcaaag atcgtatcat
    27241 cggaaccaaa tcctaacgca cgaactaata cggttaaagg aatcttacgt gtacggtcga
    27301 tacgtacata agagatgtct tttgcatcag tttccatttc tagccaagct ccacggttag
    27361 ggataacagt agaaccgaat ccttcttttc cgtttttgtc gacttttcca tggaaataga
    27421 cacctggtga acggactaac tgagaaacga ttactcgctc tgccccgttg atgatgaatg
    27481 ttccttgttc tgtcattaat gggaaatcac cgaagaatac ttcttgtgct ttgatttcgc
    27541 cagtctcgcg gtttgtcaaa cgcaatgtta catgtaatgg tgcagagtag tttgcatcat
    27601 gcgcacgagc ttctgctact gtatactttg gttctttcaa ttcataatct acaaattcca
    27661 aagatagatt gccattgaag tcatcgattg gcaaaatgtc ttcaaacatt tctctaagtc
    27721 cctcatctaa gaaccattgg taagagtctg tttgaatttc gatcaaattt ggtaattcca
    27781 acacttcact gattcttgcg aaacttctac gttcgcgatg ttttccgtat tttactacgt
    27841 gtccagccaa gctcttcacc cctctaaaat gtcattcaaa aagactaccc gatcaattgt
    27901 tacaataata tttcaaatat tatttttagg taacaacaga cattttttgc ggcttttagg
    27961 caaaaaaaaa ccaaaaatag aacattctct tgaaacctta cttcgtgagc ttctactttt
    28021 gtttccttat attaaagccg aaacggacaa tatttcgttt ctgccttttc ttcatcagat
    28081 a
//
LOCUS       DANKKG010000022        27879 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000022 DANKKG010000000
VERSION     DANKKG010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27879
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.022"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(433..1377)
                     /locus_tag="PW004_000819"
     CDS             complement(433..1377)
                     /locus_tag="PW004_000819"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HDL2725809.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1673..2617)
                     /locus_tag="PW004_000820"
     CDS             complement(1673..2617)
                     /locus_tag="PW004_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2725810.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2838..4052
                     /locus_tag="PW004_000821"
     CDS             2838..4052
                     /locus_tag="PW004_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2725811.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            4074..6089
                     /locus_tag="PW004_000822"
     CDS             4074..6089
                     /locus_tag="PW004_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HDL2725812.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(6172..7545)
                     /locus_tag="PW004_000823"
     CDS             complement(6172..7545)
                     /locus_tag="PW004_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDL2725813.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7571..7855)
                     /locus_tag="PW004_000824"
     CDS             complement(7571..7855)
                     /locus_tag="PW004_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2725814.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7857..9872)
                     /locus_tag="PW004_000825"
     CDS             complement(7857..9872)
                     /locus_tag="PW004_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDL2725815.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(10051..10356)
                     /locus_tag="PW004_000826"
     CDS             complement(10051..10356)
                     /locus_tag="PW004_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HDL2725816.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10445..11476)
                     /locus_tag="PW004_000827"
     CDS             complement(10445..11476)
                     /locus_tag="PW004_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL2725817.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11683..12558
                     /locus_tag="PW004_000828"
     CDS             11683..12558
                     /locus_tag="PW004_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL2725818.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12710..13552)
                     /locus_tag="PW004_000829"
     CDS             complement(12710..13552)
                     /locus_tag="PW004_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL2725819.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13644..15521)
                     /locus_tag="PW004_000830"
     CDS             complement(13644..15521)
                     /locus_tag="PW004_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HDL2725820.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15518..16939)
                     /locus_tag="PW004_000831"
     CDS             complement(15518..16939)
                     /locus_tag="PW004_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HDL2725821.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16942..20331)
                     /locus_tag="PW004_000832"
     CDS             complement(16942..20331)
                     /locus_tag="PW004_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDL2725822.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20588..21979
                     /locus_tag="PW004_000833"
     CDS             20588..21979
                     /locus_tag="PW004_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2725823.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(22052..22699)
                     /locus_tag="PW004_000834"
     CDS             complement(22052..22699)
                     /locus_tag="PW004_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HDL2725824.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22707..23393)
                     /locus_tag="PW004_000835"
     CDS             complement(22707..23393)
                     /locus_tag="PW004_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2725825.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23399..24472)
                     /locus_tag="PW004_000836"
     CDS             complement(23399..24472)
                     /locus_tag="PW004_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2725826.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24694..25368
                     /locus_tag="PW004_000837"
     CDS             24694..25368
                     /locus_tag="PW004_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2725827.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25373..26713
                     /locus_tag="PW004_000838"
     CDS             25373..26713
                     /locus_tag="PW004_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL2725828.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26862..27848)
                     /locus_tag="PW004_000839"
     CDS             complement(26862..27848)
                     /locus_tag="PW004_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HDL2725829.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8134 a         5267 c         5049 g         9429 t
ORIGIN      
        1 gtagatttaa aaaatgataa acctctctcg aacggttttt tatcaaaaat cgtcattttt
       61 ccatcaaaat ctgcttatgc aaaattataa aaaaattttg cagtttttct tacttttttt
      121 gtcgtttaat tgaatgataa aaaccagttt ctttattcag ttaatcaaat atgaacgaat
      181 tagatatgaa tcattgtatt tgccgttgcg gaaggatttt ttaattaacc acagataaac
      241 attgataacg attcgcttat tgtaatcaac agattaaatt tcatatgttc atatgtacgg
      301 atacattttc ggaccgatta attttaacca ttctatttat ttgcttacct attagatatc
      361 aataaaaaac tgcttaacag ctatttcagc tgttaagcag ttttaggatt tttagttcat
      421 attcattgtt tcttataata cagtgttcaa tacttctctt aaagcacttg ttgatttgct
      481 gaacattgct ttttcttctt cgttgattga taattcaaca acttcacgaa caccattttc
      541 gtcaacgatt gatggcacac ctgtaaagat atctttttcg tcgtattcac cagttaaata
      601 agcagataca ggcaatacag cttgttcgtt attcaagata gcttttacga tacgtgcagt
      661 actcatacca ataccatagt aagttgcttt tttacgatca atgatctcat aagcagcatt
      721 acggacttta tcagcaatca catctaattc atcttgggca atacgatggt ctttttctac
      781 aatttcaaac actggtttac caccgacagt tgtatgtgac catgcagcaa cttctgaatc
      841 accatgttca cctaagatat agccgtggac gctgcgtggg tcaactttta atttcaatgc
      901 gatttcttta cggaaacgag ttgtatccaa tgttgttcca gtaccgataa cacgtgatgt
      961 tggcagacca gattcgttcc aagcgatata tgtcaaaata tctactgggt ttgaagcaac
     1021 aacgataata ccgtcgaatc ctgatcccat gatttctttt acgatttggc gcatgattga
     1081 cgcatttgtt ttaactaaat ctaaacgaga ttgaccaggt ttttggttga taccagctgt
     1141 gataacaacg atatcagcat ctttacattc ttcatagcca cctgaccata cagccacatt
     1201 ttcatcgccc caagccatac catctaaaag gtccaatgct tccccttctg ctttttcttg
     1261 attcacatcg atcaatacta attcattaga gattccttgg ttgatcattg cataagcgat
     1321 acttgttcca acaaaacctg taccgacgat tactacttta cgacttgttt ttttcatgaa
     1381 taaacagctc cttttcataa ctatttgtta ctttcatgaa aagtatagtc ttgtttattc
     1441 attttatcaa catgttaatt ataatagttg ttattataat ttttgatgga aacgaattcc
     1501 tgaatacgca taacagataa atgacaaatg tttacaccta tatcccctga taatacaacc
     1561 aaaagtttag aactgaaaat aaatccaaaa aaattcgctg caacgatata ataagacagc
     1621 aatcgcacct aaaacaattt tccttcatat gctttagtga aagattttct tcttaatgtt
     1681 tatttctcca ttcattggga gtacatgttg aatacttctt gaataatttg aataaagaag
     1741 tcgtttgttt atatccgata cgattagcga tttcttccaa cgaaaaactc gtttcttgaa
     1801 tcagttccaa cgcaaagtcg aattttttct ttagaaccag ttcttgaaag gaagcatcaa
     1861 aatacatttt caatttatta ctaagataga ccggattgta gccaaaatga gctgctgttt
     1921 cttttaacgt acaagaagta aaatgcttat cgatataatc taatactcta agcatctcat
     1981 tttctgcgca tccatctgct gatgaaaagc tttgttgttc taataaatga gtcaattcga
     2041 tcagtaatag tgatgtatag agatttaacg aacggatctt atgactgtat gtatcaaaat
     2101 aaatcaacag catattttca atcaaatgcg tcaaacgact tgtttttccg ccgcgaaaat
     2161 gaatgaattg tgtatgttcc cctttttctt ttgatgcatt tatcaaaaaa gaagaaagta
     2221 gattatttgt gcttgccaaa tgattcaata tctctgtaga taaggtttct tcttttaata
     2281 agatgttgat taaaatatcc tcttgccctg tgtattcgat tctttgagta gcttctttat
     2341 caatgatcac cacgtcactt tcttccatct ctatcctttc tccattaata atttgtaaac
     2401 atgtaccgct atacatataa ttgatttcaa caaaatcatg tgtatgctcg ggaacttccg
     2461 catatcgacg gtgtttactg atatgaatat ttccctgttg aaatagatcc cctcggtgga
     2521 tttttggcgg tgtctgaaga tggtaagcta agtttaaatc attgatattc attccttgtt
     2581 cacgctgcag agtttcgatt gattcttcgt tacgcaaaaa aaatgtctaa ttcagctagt
     2641 tccaattgac tccctccttt ttgtaagcgg tttctttcgc ttattagttc acgctgtact
     2701 gctattatat gcaaagctat ttttggtttc ataaagactt tctatggttg ggacatgaac
     2761 ttcctgtgat tccgctattg agagaataac cacttcatac tatatttttg ataagcgctt
     2821 acaaaaatga aaggaacatg cttatgaata aaaagatgat gaaattttct ctgctgtcag
     2881 tctctcttat tttgacttct gctggtgcag tcacgtctgc aattcccagt atgtcagcta
     2941 cttatgccac gcgttcttta tctgctgttg aattattaac gactgctccc tcttttatgg
     3001 tcactatttt tgttttgatt agtagtttta ttgctaggaa aatcggacaa aaacaaactg
     3061 tgatcatcgg attagtgatt gcatcgattt gtggtatcct ccccctcttc tcacaaagtt
     3121 acagtattgt cttagcttca agagctggac taggtattgg ctttggattg atcaattctt
     3181 tggcagtcag catgattgct gccttttatc acggagatga acgagcctct atgattggtt
     3241 ttcaaagtgc atttcaagga tttggtgcag ctttaatgac atttgttgca ggacagctag
     3301 tacagttcgg ttggcaatat acctttttag tctacgccat cagcttacct attcttgtct
     3361 tgtttgtttt gtttgttcct acacctgaaa caactgaact tcaatcgcat ggcacagccc
     3421 caacaagaca agtaatcaaa acgaatccta aaatgattag ttatggtatt tttctcttgc
     3481 tggtcatcgt ggtatataat gctgtatcga ttaaattagc cacagtcctg acgacttatt
     3541 ctctaggaaa cgaaactaat gcagcgaata tcttaacaat catgcaattg gcaagtatgg
     3601 ctgccggagt ttgttttggc tggcttcaga caaaagcaca tcgctatatg cttacacttt
     3661 ctttactact gatgggaatc ggctttgtat tgattgccac tgtgataaac ctgtatgtga
     3721 ttgggtttgg tgcgatccta acaggaattt ctttttcgtt attcattcct tacttattca
     3781 accaagtaag tatccaagcg cccaagtacg cggagagttt caatacttca atcttattgg
     3841 ttggcgctaa tttaggtact ttcattgcac catatggttt gctctttctc tcttttgctt
     3901 ctcttgggag taaaacactt gctgttttcg tcaatggcag tatcttgctg ttgattagcg
     3961 cgcttattag ccttctagta gtacacagga cgaatagaaa gacagtgata agagtagatg
     4021 aagaaataaa agaacaattg acaacaaaat aaatttgatg gaggtcgtgt aaaatgacaa
     4081 atgaaaaaca taaagataga tggctttggt atcctggaga ctttgagatc cgccatggac
     4141 tgctacaaaa tttccaacgg gaagaacgag gttttgactg gccagcgtac tggtacatgg
     4201 atgattgcca tcggaacgtc aagtttaaga gatattactt tcttgatcaa ccttccatgt
     4261 ttaaagtgac gatccaagga gttgggtatg tagaaattaa tggacaaaaa catccatgtg
     4321 gaaaatggct gacttgtcca gctggtaaag caaaaattcg tatctttgta ggacatacat
     4381 cagggctacc agcgatgttt atcgagggag acgaagtaaa atcagatatt ggctggacag
     4441 caagtaattt tattgaagaa tatccagctg gctggagtcc gctttatgtc gatatcgcaa
     4501 aagatccaaa tcaaatctat tatcaaaaag aatgcgtcca tccagtcact gaaacggaga
     4561 ttggcggagg aatcttattc gattttgggc gagcagtgaa tggctgtgta gaactaatat
     4621 ctctggatga ccatatcgaa tctatcactc tatgttatgg agagtccgat gtagaagcta
     4681 tggatatcga ttattgctac tataagcaaa taggaatgac aagaaaaatg aaagctcgta
     4741 aacgcgcttt tcgctatttg tttataccag aggtttctac tggaatggtt caacttcaag
     4801 tttttcatga atttttatct ctttcgaaac aagctgcttt ttcaagcgat tcttctttac
     4861 tgaatcagat ctggtcagta tcagaagaag ccttcaagtt atgtagtgat ttgtttttta
     4921 tcgacggtat caaacgagat cgctggattt ggtctggaga tgcgtatcaa tcattcatga
     4981 tcaatcaata tctttttttt gacgaggaaa tcaataaacg aacgatatta gcattaagag
     5041 gacaaaatga aatcaagcag catatgaata ctatcgttga ctattcgatc ttgtggttga
     5101 ttggtatcga aaaccactat atgatgacga aagactatga atttttacgc atcgtctacc
     5161 ctaagatgca atcattaatg agatacttga tggagcaaac gaatgaatta gggtttattt
     5221 atgggagagc aaaagattgg atatttattg attggtctga aatggataaa gaaggaacgc
     5281 ttgctgcaga acaagtctta ttactgaaag cctatcattc catgatcact tgcggagata
     5341 tcttagctga aacatatgga gagaacgaaa attatcatac tcatctctat caagaaaaat
     5401 atcaactatt gaaacaaaac ctatttgact acttttggga tgaagaactt ggcgctttta
     5461 tcgattgcta tgaatctggg agaagaaatg tgacgcgtca tgcgaatatt ttcgctgttt
     5521 tattcgattt tgctaacaaa gaccaacagc aatcgatctt aaagaatgtc ttattgaatg
     5581 acactatcac acagattact actccatatt tcaaattcta tgaacaagat gctttatgca
     5641 aactcggaca gacgaatatc gtctaccaaa cgattcttga ttattgggga gggatgttat
     5701 ctcatgatgc ggtaactttc tgggaagaat acgaccctac tcaaacagga aatgaaaagt
     5761 acagtatgta tggagatcct tatggaaaaa gcttatgcca tgcatgggga gcaagcccaa
     5821 tctatctttt aggacgctat tttatcggtt tgtatccaac aaagccaggt tatgaagaat
     5881 ttatgataca acctaatcta gattcatttg aaacactgaa ttgtacgcta cctatcaaag
     5941 atggaaagat ttcgcttgaa aaaacaaaag ataagatcgt gattacttct gatagaacag
     6001 gcggaacagt gaaaatgaat ggtaaaagtt atcctcttct agcaaaacag ccacttgtct
     6061 taacaagcta ttcaaaaata aatgcataaa acaaaaaact gcttgcgaca tatcagaaga
     6121 ctttaaatct tcttctgata ttgtccaagc aatttttatt ttgaagtttg cttaattctg
     6181 ttctttagaa acaatgtttg ttctttttcc ggcaaaaaga aagctagata atacgacaag
     6241 taaaaagaaa gcgatgattc caactgtcaa ggctatttga ccgtattctc ctagtttccc
     6301 aagtacaagt cctggtacta gataatcagt atcggcaaat gtagaagaag cgattttaag
     6361 atctcctaat actggaagga taaataatgg cagccaacta atcaataagc tattgaaagc
     6421 agaacctaat accgcccctc ttctaccgcc agtcgagttg ccgaagacac cagaagcagc
     6481 tccgcagaag aaatgtccta ctaccccagg aatgataact gtagtttgca agcctatcat
     6541 agcaaacatg gatagtgttc ctacaaagaa gctaacaaaa aagccaatta atacagcatt
     6601 aggtgcatat ggaaaaacga taggaacatc taatgctggt ttagaatcgg gtacgagttt
     6661 tttagcaatc ccttggaatg ctggaacgat ttctgccaac accattcgaa ccccttgaag
     6721 gacgataaca aacccagctg tgaatgttcc agcttggatc agtgcaaaga ttacagcgtt
     6781 gtctccattg cttagttctc tttcaacaaa tccaggacca gctaacaaag caagtgcgac
     6841 atatacgatc gacatcaaga gcatgatcga tacagtagaa tctcgcaaaa agctgaaact
     6901 tttaggaatc tttatgtcct ctgtagaacg gtctttgttt ccaaaaaact ttccgatcca
     6961 tccactagaa gcataaccga tatttccggt atgccctaac gcaacttgat tgtttccagt
     7021 gatgttcttc ataaacggtt gacaaagagc aggcgtaata gttaatagtg ttccttcgaa
     7081 gaccccgcct aataagatca tccacaaatt tgcagtaaac cccgcgctta ctaaaatgac
     7141 tgctgttaga caagaaacat aaagcattgc ttgacctgta agaaaaatga atttatatct
     7201 tgtgaatcta gcaagtaaaa tattcacgat catccctact agcatgatca aagcagtagg
     7261 tgtaccgtat tgttgcaaag caatcgccac tactgcttca ttgctaggaa ctactccttg
     7321 agtatgcaga gctgtttgga acatcgaagc aaaaggggtt agtgaattac tgattaaacc
     7381 agcaccgccg atcagtacta ggaatccgac aaatgttttg atagttcctt ggatcacttc
     7441 tgaaaatttc ttcttctgaa gaacgagacc aagcagtgca atcaaagaaa cgaggatgga
     7501 gggctgactg acgattgaca caaacaaatt caacaattta tccattgata gttcctccct
     7561 tgaatcattt ttattctttt gacaagatct tcgttacttt ttcttttact tcttcgctgt
     7621 caatgatcga ttgcaaaggt acgagctttt catcaggtat agaagttagc gtatctttca
     7681 aagaatgctc aatgaagaaa taatcagcca tatcgctaga tacacttcca acatccgtat
     7741 gacttacttc aaactcgtcc acatctgctc caagatctgc aagtgcttgt ttgatgttca
     7801 tctctaccat aaaacttgtt cctaatccac tttgacatac tgcaaaaatt ttcatatcat
     7861 tcgtctcctt ttcttatgat cgctaatatt tcttcttttg ttgatgcgtg taataaatct
     7921 tgaagatttt gtttattaga aagtagcttt gttaaatttg ccaaagcccg aagatgtgcc
     7981 tcattatcga tagcagctaa acagatgaac aaactaatgg gatgggctgg attatcaagc
     8041 agatccacag gttgttgaag tttcagcaaa gacatcccta cttcgtttac gccatcttct
     8101 ggacgagcat gaggtaatgc gatgtgatcg ccaatatgga taaacgcacc atactgctcc
     8161 acgcgctcga tcatagcttt gatataactt tcttctattt tattttctga taatagcggc
     8221 tgtgcggcaa atgtgattgc ttcttgccag ttttttggtt gatccgataa ttggatcatt
     8281 tcttctgtta ataattcaga tagcatagga cgttgatcct ccttcttttc taaaagtttg
     8341 ttcattttac gatttaatac ttgatagagc ttttcttcgt ctacgccttc ttttaattcg
     8401 atataaggca gtagcgccga caacaattct tgggcagaag gcattgaaaa tcctggtatc
     8461 agccaatctc tttgtacttg attgatcagc tgattttttt ccaagtcagt catgattgga
     8521 tgcagaacat agagagactt tttcttatca gtaagtgtga gaggtacagt agaaaaaata
     8581 atatcgtaag aagtttcgtc aacctcgtgt aagcggtcag ctgaattagt ctctgtaaaa
     8641 aagatcatcg ggaataattg ctgaagttca ctttttaaga tcaatgaaga gctaatgcca
     8701 cttggacaga cgatcacggc tcttattttt ctatttcttt cttctgcgtc cgcttttctg
     8761 atctctccgc caaatagtat agtgaaaaaa ccaatctctt cttcagggat gctcttccct
     8821 attcgtcttt ctaacggaag taatgctttt cttgtcattt cgtagatgga agcatattga
     8881 tgcttaattt gatcgatcat cacattaggt aaataaaaag aataggaaat acgaaaatag
     8941 gcagggacca agtgataaaa gacattcatc aataattcac gaaacgaagc aaattctacg
     9001 gccatcagga attccatgcg atggatgact tctgaagaac attccagcaa aaaatcaagt
     9061 gcaggctcct ggacatctcc ttcaacgatc gatgctaagc aaagagagaa aaatataatc
     9121 tccgaatttt gatgttcgga atagccaagg ccagtcaata cttgtcgact aatgtcagtg
     9181 actgcaagag aagccaacag ttcagcatct tttaccagtt ccatggatgt ttttccaatt
     9241 cgaatggttg tcaaactcaa ataataagct aattcatcca ttcgatttgg tgcgaatcga
     9301 agatctgata gtgaaatcgt tttatctagc aaatctctaa ctttggcgta ataatcaatg
     9361 gaaagggttg cggttaaata cgtgagccca aatcttccag caagtgactg catcaaacga
     9421 gcagtgcgat tttttgcttc tcttcttaat attgcttctg accctagcag cctaaaacca
     9481 gttttcctag tatacgacag ctgacaatct tccacttcta attcatttct tatctttttg
     9541 atatctgaca ggactgtccc tttagaaacc tgaagaaaat cttgaaaatg aaaaacagaa
     9601 acagacgttg cagtcgaata aaccatcaaa taaagaagat ctcttcgctc ttgttcggta
     9661 tacacagctt caaatcgagt aatatccaaa aatttttctg cccagtcgat tgctcggaat
     9721 ttctttatac ggatacgggt atcatccaca tattcggcaa aaggagttaa ttgtttgatc
     9781 gcctcgcttg tatttacttc gatgatctct gctgcttttt ctagactgat ttctcttggt
     9841 gttttcagga gataactaat tttgtagttc acagcgctgt tcacctccta cttatattct
     9901 agtatggtag cgcttttaaa tcgaggggtg tttggtacaa cttacagttg gatttttttt
     9961 acgactgtac cgaacgattt ctatcgaatg gatacagaaa aaaagcaggt gaccaaatgt
    10021 ctcctgcttt tatgaattga tttcgtccga tcaatgatga tcatgtttat tttttgaggt
    10081 cagttcttca aatttttctt tgacatcttc aaccgctttt tcgatatctt tttcaaagtg
    10141 attgggctca ttaggctttg tttcctcttc tacagaattt gaaccagata aagctgtttc
    10201 tttattttct gtttcaccag agtttgtgcc agctaatgat tcttctttgt cttctgtttc
    10261 accagaatta gttcctgcta aagccacttc ttcttctgtt ttaggggcca ttgaacctct
    10321 taatcgtttg tgtttttcac cgtcataatt tgtcatgttg gttcctccta gtagttctat
    10381 actttttcat cgtacaataa gacagtttat gcgtcaaaaa acaaacccga ttcttacaaa
    10441 tatgtcatat ttttccttct gattcttgaa tgcttttgat tccttcttcc aaaattttca
    10501 tttgtaagat tgtttcttca tctttgatga ctttttctgc gccattaact aatgtctcgt
    10561 aaatcccttc gtagacacgg ctgtaatcac caacctcgga aattactttt tcttcatgat
    10621 aactcccttg ttcatctata taggtcaata caccataatg ttcgggcaaa tcgataccaa
    10681 agtcttcatg atcaggcatg taaaacattt tcagatgttc ttcttgtcga tcttttgttt
    10741 gtttcacgaa catccctttt tttccgtaaa cgacgaaact tggacgttct ttgatgcgga
    10801 aataactgga ttttatcgaa attttcatat ttccatagaa aaaatccaga tcgaagtaat
    10861 cattcattcg tcctttccct aaaagttgac gtacatcata gctgatatca tctggttcgc
    10921 cgaaataaga aagaacttga tctactgtat gacaagcatg tccatataaa tagctgttcg
    10981 cagtagaaaa ttcatgcgta ttttctggta cttccggtct gaaataatca taatgcattt
    11041 ctacttctaa aatatcccct aattttccgc tttcgattac tttttggact gtcaagaagt
    11101 cagaatcgaa ccgtctgttt tggtaacatt ggataaatag acctttttct tttgctaatg
    11161 caaataactc ttctgcttcc tttgacgttt ctgcaaaagg tttctctact agtacattct
    11221 taccatgttc gagtgccatt tttccatatt cataatgaaa ttgactaggt gtagtgatga
    11281 caactaattg gatctcagga tcatcccaaa gatcagacag ctgatcagta tattcgatgt
    11341 cttcgaatgt tttccacact ggttcagatg gggaaaaaat ttttttgata ttgattttgt
    11401 tcgtaagttt tcttgaaaaa ggcaaatgat agcgatttgt actctttcca ttgccaagat
    11461 aagcgattgt aagcatgagt ggtgcctcct tttattgatg ttttcagtat atcctagaaa
    11521 agcgcagaaa gtatttataa tagctattct ggtaaaaaag ttcagaaaaa cacttgttat
    11581 cattcggttt ggtgaaatta atgttcaaag taaggataaa aatcaaaaag atagtagcgg
    11641 aaccaagtgt ttatcagtaa gatagaattt ggaggtcaga tcatgcttat tgaagaaaaa
    11701 ctaacaaaac aagagttgtt taccacgaca gagaaacgga ttgtcgatta catccgtagg
    11761 aatatcgaag ctgcagtcta catgacgatt gaagaattag caaaagctac gtatacttcc
    11821 cattcagcta tcattcgttt atgcaaaaaa atgggattca gcggatttaa agaattccgt
    11881 ttcgagctgg ggcgggaagt tcatcaattg attgctcagt tcaatcaaac gattgatcct
    11941 aattttcctt tttcaagtga agatagcact cagcaaattg caaagaaaat ggcggattta
    12001 tcgatccagt caataaaaaa agcacaattg cagattgaac atgaagattt agatcaaatc
    12061 ggtaaagtat tgaacaaggc acagcgaatc tttctttttg caaaaggtga ttctcaaatc
    12121 acggcaagga aattccagaa taaaatggtc aagttgaata agtttttgat catggctgaa
    12181 gaatacagtg attcttcttg gaatgcagcc aatttgacta gcgaggattg cgccattttt
    12241 attagctaca gcggtcgcat ccatcattat gaacgaataa tgacctattt gaagcatgta
    12301 gaggccccta ccttgctgat taccggaaat cagcattcag aaatggccaa acaggctgat
    12361 atgtgtcttg ttatatcgca ggaggaatat gattttgcta aagtagcgac tttctcctct
    12421 caaattgcct tcgattacgt attaaatacg ttattctcag tgatatattc tcaaaatttt
    12481 gaagagaata tccgcaactt gaaaaacaaa caagaattga tgcaaaaagg ccttcttaaa
    12541 gaatatcgga aaacttaaga aaagatcctc atttagtaat aagaaagata gtgtttgata
    12601 ggctctcttt gatatcatca taaaaacagg ctgaagtttt ctttttcaaa cctcagcctg
    12661 tttgtttacg gaacgattcg aatagatata ttatagaaat gaggaatagc taacttctgt
    12721 ttcttatttt gaacgattga cgaaaaagat atataagaag gaggaaaatc agtatacatg
    12781 cagctaacaa tccatacagg aaaaatcgtt tgttctcgga tactttctgt acttcttttt
    12841 gaacttcttt tgcatgataa ggaacccggt gccctctgac aagtaagcga tgactgttga
    12901 ccatataagg tgtacaagtt aggagtgtca cgaaatcctg ccctttttcg attctcagat
    12961 cttttgtatc agtcggttct accgtcttga tctgatcgac ttgataagcg agtgtccgtc
    13021 cattgatttc gatatagaaa gaatcaccta aagtcagttt cggtaagtct gtgaacaact
    13081 ttgcttgagg cagtcctcga tggctggaaa taacggcatg tgtattttct ccccccgtag
    13141 gataagacgt tccttccaat aatgaggcac ctttttctag cagcacagta ttcgtttcat
    13201 caaaaattgg caaatggaca ttgatctcag ggatcgtcaa aataccgatc gtatgtttct
    13261 caaaataggt tctatccttt ttcgcaggtt cttttttctt agtaaaggga tctgcacctg
    13321 gatttccacc ttcttttgct aattgctggt ttttctttgt catgttttct tggattttag
    13381 ccatgacttc ttcattttct ttgaccgctt gttgctgata atgagagatg atctgctgat
    13441 ccagataatt attcagcgcg tcgctaacaa atggataagc tagtatccct attcctgaga
    13501 gaagcaagaa aagcatcagc agatctagaa gtttcctttt ctttggtttc atttgtgttc
    13561 tccttgtgat gactgtttga taataaaaga aaaaggagca tttgcgtaat ccgctgctcc
    13621 tttttccaat tcttgcaaaa agactaaatc tgactgtgct tgcgtctagc aaagtacagt
    13681 ccagcaatca gtagaaggac tactcctgca ccgatataca catagattcc cttaccgcct
    13741 gttgaaggaa gtgtaccttt gtgtttattt ggtatttttt caggagaaat caactgtcct
    13801 gctgttacat aagattgttc atcgatagta aatgctacac ggtttgttaa tggaacatat
    13861 ttttctggcg ctttcgtttc ttccagatag tacgtgccat attttagacc tgtcacatcg
    13921 attaaaccgt tacttgtggt tgtaaaaacc gttgctgatt ctttcgccga tacccagctg
    13981 acggcttttg tggatgggtc gatcgataaa tatttcgctg tgtcactatc ttgatcacga
    14041 acgacgaatt ctgctccagc aagtgtttgg tctgatgtaa cgtcaccatc tactttaaca
    14101 aatcgtttgc ccccagtaac gacatcgact gacggtggtg tttgatcatt tgtatgcccg
    14161 ttatcgacat ttgcttggtt agaaaatcct ttggttggat ctgctttttc gttcaaatgc
    14221 atatagtaaa cgaatttcaa tgtaccgcca ggagttaatg aaggaatata attcggatca
    14281 actgaaaccg tgaatccgtc tgtttgctca gtgacagaat aatttactgg gtcgatttct
    14341 ttatttccat catataaggc ataagcatac gttcctgaag aatcattatc aaatgttaaa
    14401 gcagcgtcat gagtatcgat aagtttgaat gttgtgtact tgttttgcgt tccttcttta
    14461 tcagcgatac ctaatgggat attgacagaa atctcatatt gaattttttc accgattgcg
    14521 acatcttttc cattcaattg aggtgttgtt ttatcaactt tagacgtatc atttttgatg
    14581 gtcttttcta ctggtgtttg gctatttgcc aggatctcaa aaggcgtgat tgtttgcttt
    14641 tcaatcattt ccgcattatc aggagctttt acttcttcta aattatattt tccaacttct
    14701 aaatgattaa cgatcaactg gcctttttca atagatgcct cggcaaagtc attgttgccg
    14761 atgtcataag aatgaccagt aatgaaatgt ttggctttgg tttgatcagt tgtccaagtg
    14821 tacaatccat ctgtgacact ttggatgtat tttttgacgc ttggtgttcc ttcttcttta
    14881 gaaataataa attctgctcc atttagtgct tcgttttcgg cagtaccgat ttttgtaact
    14941 ttcaacgttc catcattacc gactgtattt ttagggtaga gatggatagt atctagttct
    15001 tctgtcccgt atttataaga gccatctgct tgtttgatca tctcatatac agggaaagct
    15061 aaaaccatgt ttgcggcagc tgacactccg tcttttggtt cttctttgat cgtgtagact
    15121 gcatctttcc cattttgttt tttaggtaaa gataaagtga catttccatc agcatctgtc
    15181 gttcctgaag caactggtgt accaggagtc aaagactgga ctgcttgttt tgctgcatcc
    15241 acggacgctc ctttatctcg ttgcgcataa aattcttgag tgacgttata aactgaaaat
    15301 gaaatatcgg ctaatccttg gtattgatcg aattcgctca tttctttccc gctgttttgg
    15361 attaaaggat cgggtaaatc agtcattttc tttttatgaa ggatgacttg agctgctgtc
    15421 tcctctgcaa acactttttt tgcgccgaag ctgtttgtga gtaatggtaa aataaggaaa
    15481 aggactccta acataacgtt tattttttta tgatttttca ttttttcact cctgttcttt
    15541 ttttgataaa caaccagatt gcaaagcttg cgaataccat accaacaacg cctaaaagtg
    15601 tatttttcgt ttcgtttgtt tttggaagaa atggacggct attttctttt ttattgacta
    15661 ctttcaatat actgtccgcc tctttatcct cagaaagaaa agttgaaatg gtaaacggaa
    15721 tttctgtttc acttaaaata taaccagaag gtgcttcgat ctcttttaat cgataacttc
    15781 ctgttttcaa cccggaaatt gaaaaagcgc catttttatc ggaaatcagg tgaagcgcat
    15841 ctgattcgtt ctttgtccac ttgtatccgt tggctgtttg ctggaggtaa tttcctgctt
    15901 tgtttttaat aaggaatact gcgccagcta atcgtttatc tggtttattt gtctcgattt
    15961 tgacaaattg cttactccct gtatatacag agactttctt tgtactgaca ccaaaaccaa
    16021 aatccagtgt tccgttgttg ttgatttcct tgttcgcctg tgctgtactg cttagctgca
    16081 tctgataaga aatggtcagt ttttttccag catatttttc caacgaaggg atcgaaaata
    16141 aaacatcaaa tccgtgaggt ttcttgtgta tgacaaagcc ttctgtcagc ttttctccat
    16201 tcgatgaaat cgttaaacta ttaggtaaaa acgttaatgc ttcatccgca ctatctgaca
    16261 attcgaactt ttgatagtca aggatattta caggaattgt cgtatgcaag gaataagtga
    16321 ttttttcacc aatcgtaaaa tcatttctag gctcttctaa taccttctca aaaggtggat
    16381 cagggtagtc gttttcttca tttttaggat acaaatggat agttgaaagt ttttgattgt
    16441 tttgtccatg tacaggaaga acaactacca tattctctgc cttttctttg acgacttctg
    16501 gtactttgga ttcaatgaaa agatacgctt tatctctttt ttcttgatct ttagcggcca
    16561 gagaaaaaga agcgatacca tcttcgttgt taagagttgt agttgttgct tctgcggtaa
    16621 acataccgga agacgcaccg ttttttgcga tctctgcttg tgcttcttct accgttttgc
    16681 ccttttcccg tagatggtaa aaagaatctg tgacatcata aacttggaat gtgacaccat
    16741 ttaatcctcg atacgtttgt aataaagctt tttcttgtcc gtcatttgga tgatttttcg
    16801 gcagttgccc gtttgggaaa agcaatttgt gtaaagtaat ctgaaccatt tctgtttctg
    16861 tttctgttgc tacctgagta aaagctggtt taaatagtaa caagaagaga cacatactaa
    16921 gccaaccaag ttttttcata tttagctgcc ccctctttct tttttcaaaa aaagatacca
    16981 aatagaaaac gcacaaccaa tcatccctac tagatagatt cctaaacggc caattcctcc
    17041 cgtttcaggt aatggtactt ttgcctgatt cgtgatgtct aaagaaatct ggttgttttt
    17101 ggctcctggt gacagaacag attcatgatc ttgtccatcc acttgaattg acccatcttc
    17161 gtgtataact atagtaactg gctcttttag accttggtat ccttctggtg taaaagtttc
    17221 ggtcagcgta taagttccag gttttaaatt ttcgaataga aaggtatctg tttcttgtcc
    17281 atcttttggc gattctaagt ccatttctgg tccctgcagt ctgaattttg ctccttttaa
    17341 tgtctgtccg ttgtcttctt ttttgtgtac cgttagatca aacgctttta gtcgattttg
    17401 atgagtgagc gtccatacat tatttgtcat ctcggtattt tttcccgtgt aaatgatttt
    17461 tccatattta tcgatttgga attcataatt tcccggcgaa gtatcatatc cggcaggacc
    17521 agctgtttca acgactcgat atttaccagg tgtgagtgaa tcaaaactgg caagaccttt
    17581 ttcattcgtt gtttggctgt caattggctg ataagtacca tttgcttctt ttttctgtag
    17641 ggaaaatgct gctcctttaa gatttatctc tttgtccgtc ccttgcatcg catacttacg
    17701 tactgctaca ggaacttcaa caaaaggatt ttcaatagtc aactggatcg tatcatcgga
    17761 agtagtgact gtttttccat caatggttac cgtaccatca gaagcgatct tgatttccca
    17821 agtagtcttt ttgcttaatc catgcccttc tggagctttt gtttctgtca gcgtataggt
    17881 catttctttt tgcaatttga cattttctgg aagagaatag gttccgtcgc catgatctgt
    17941 tagtaaagta tcaatagaat ctcctgttaa tttgaaaaca gcgccaataa gatccttttc
    18001 acctgtagag gaatttttcg ttattttcaa gtttaacggt gtgaattgtt tagtattttt
    18061 ccatgtcatt gcatctattt gttgagaatc gtatcctggt acaggtaatt ctttgattgt
    18121 ttggtaactg aatgcttgac cttgattatt gtattgaggt agtgataata tctcttgata
    18181 actttctccg ctatttgcag ataatttgtc aatgtctgca cgttcccacg tatttgttgt
    18241 atctttagct ggtttaatga ttcgaatata tccgtttttc caagctgcag catttgtcgt
    18301 atgttcccgt tgaatctcaa aagtaacttg atctggacga tcagctagat tgttgtcaaa
    18361 ttcttcccat aactttttga tgtgaagact gactccggga gctttagcag atggtatacc
    18421 gaattcagct aattcattgg tatcaatgtt tggctggaaa gttgtcctgc cgttcatttg
    18481 ataccagaaa tttggatgga agtcctcatt ttctgtttgg attctcactt ggtaatggat
    18541 ttggatttct tggtcttttc ctaaatagat ctgattgctc ttgatggtat ttccttctat
    18601 ggaaatagat ggagtgaccg ttgtaggact tgtccccaca ctcttgactg aaagtgtacc
    18661 aggctgataa atgaatggtt ctgcgattgg atcatttatt tgtccattgc ttacagttgc
    18721 tgagatctgt acagcttttt tggctaggta ttcagagata tctgttgcat gatcagctga
    18781 ttcatagtaa agatcgcctt tttcatctga tgaaaccatt tgtcgcatac gagactctac
    18841 ttctgctttt gagagaccag ctgccggatc gctttgaagt tggatgccaa gaccatgtat
    18901 ttcgattcct cgttctttga gtgccatcgc ttctccgatg gttgcgataa acgtactgtc
    18961 gattcttctg gatagattgt tttggtcagg tgcatcatag attcttgaga ttagagaagt
    19021 atttcccggc cgatcttgcg tattagaaaa ctgagttccg taataattgc tgcttgattg
    19081 tgcgtgtacc cgctgtactt tataggaaaa tgttggtaca ccatccgtca gcaaaacgat
    19141 cactttttta tgtccatttg gaacggatag catgcttcct gcatctctta gtgctttttg
    19201 tgtaaaagta ccaccatttg tccgtgaagg tgtaatggat tttacttgat ttttcactga
    19261 atcaaatgaa cccatctgta ctgcaccgtt actgtagcta tatccttcgc ttgagtagcc
    19321 gacatatccc atattgattt tgtctgtgat accgctatct gctaaagtat cgacaaaacg
    19381 atcgacacca atctttactt caccgatccg attattgtcg ttcatacttc ctgaccagtc
    19441 tacgaccaat acgagatcaa gaggcgtgat atctttttgt acatttccac gagcattcaa
    19501 ataaacatca aacaatccgg gtgtacttgt ttcctttgca tactttcgta tcgcatagtc
    19561 tgcttgatta ccggttcctc catattcgat atacgaatgg gtccgatcat taggatctcc
    19621 attccaagaa tttataccat cccattgatt tgttccgttt ttattgcctt gatgatcaag
    19681 aacatttgta tttcctgtag gttgccaact ggctttagga taagtgccct gatcatctgt
    19741 tgtatattct gtaggaatcg tttgattaga tggcacactc acagctggca aggataatgt
    19801 aggcaatgta aatgggctga cagaattttc gttttctttt gatgattctt cttctatttc
    19861 gtctggcttt ggtactgaca catctgtatc tttcttattt tctgtctcat caataatgtc
    19921 atattcttta ttatcgacga tcagcttgtt tgcctggctg tggtaaagtg ctggatcgat
    19981 tggaaaactc agatcgatag tttgtttagt gtttgatgag atttccaacg taattttccc
    20041 gctatttacc tgataagcat agccatctcc ttcttttctt tccacatcac ttaagtaatt
    20101 ttcgactaga ccaatttctt tttctatctt ttcagcactg gtgttattcg catttagctt
    20161 gatgttcagt gctttttttc cagactgatc cgaaatgact tcagctgaaa ttgtttctaa
    20221 aactgttgga tcatctgtag tgtctgctat tgcagataat ggcacaaagt ttgataaact
    20281 caatattatc agcatgaaaa taactttcag tttcttccct gtggtcgtca tttttcccct
    20341 cctttttctt tttttctgtt ttgacgtgaa tagtgaaaat tattaaaata aacaggcgtt
    20401 acataatttt atttatttta atatgataat tattatacga agggcgctaa agggaatcac
    20461 accaattttc ataccaattg gtttcattta tgaagttgat tccttcgatt aatgagcgtt
    20521 tcattgaaag aaaagggttg agggagaaaa acatatgatc aggttgttgg aattaaacta
    20581 tagaaagatg gaaaaaatca ttcgaattct gtccatttct gacaagtgga tgactaaaaa
    20641 agaacttgcc aaacgaattg gtagttcaga atctacattt gtaaggtata tcgaagagat
    20701 caaaatccgc tggggaacgg atgtaacgat tcaatcttca caaaaattgg ggtatcgtct
    20761 tgaacatttg aatgtaccag cttatttaaa tgttctgatg gaaatgtcgc aaagttctac
    20821 aaacagtcgt ttattaaatg agattattgt caatccgggt aaaacgattg agtattattg
    20881 tgaaaaaatt tccatcagcc ggtcttcttt tacacgtaag ttgaaacaat gcaatcaaat
    20941 tttagaaagc tatttattaa aagtgatcgt tgaccaaggt tttcagttaa ctagccttaa
    21001 aagtgagctc cagttgcgta tctttgttac ctttttcttt ttaagttact acggaagacg
    21061 cacgttgcca tttccaatcg aaaaaaagaa gatcaaagaa attatggtca agaataattg
    21121 tcagatgaat ttgtttcagg aagaaagccc atatgagcaa tctttcttta ttatgtattt
    21181 tgttgtgcat ctcatgagag aatcccaagg aactccattt gaatggaaag ctggagactc
    21241 ttatgtacag ttgataaata tcgataaaaa agacttctta atcttgaaaa atctcttagg
    21301 aaaattgaag tacagcagct atataaaagt agtgaactat tttgtcaata cgattttttc
    21361 agaattttcg gagaaagaaa aaaatgaaat cagtgagcag atacgaaaaa atatgtccta
    21421 ctgttcgttt tcatccgctg attgcaaaga aaaaaacgaa gaatatgttg ttcaattatt
    21481 aacgcaaatt tattttttct cccgtatttt cccatacgat acgactcatt tgacgatccg
    21541 tgcaacttac tttgcacagc agatgcaaca ccgccagata cgtctatatg ctggatttcg
    21601 tgcagaactt caattttatt ccactctact gaatcagaat ctgatttatt atttatctag
    21661 ctatatcttt tggatgctta tcggtaatcc tgaaattcac cattatactt ctgataaaaa
    21721 aatactgatt atcagcgact taagtcttcg acatagtcaa tatattgaag aatatatcag
    21781 tgatattctt gctgtgcata aaattcactc tgagacaact gctattactg aagatcagct
    21841 gtcgaaatac aatcttctcg agtatgatct gatcgttacg aatcaaccca ttttgaatgc
    21901 ccatgttcct catatattga ttgatgatag tgtgtcattc gcaaatgaag aagaactcat
    21961 ccgcttattc gagttatgaa taatcaaaaa acagctatgt ataaaccacc ctgataaaaa
    22021 tttcaggaca aatacacata gctgtttctt tttattgtat tccttcagca atccaacgag
    22081 atttatagcg aaagtctttt aacaactctt cttttgtcca attatttgca atgagatccg
    22141 tcaaagtgtt cattacgagg gtcatggaaa agtataggaa actaactgct tcttccgacg
    22201 attgaatgga gaaatgattg attcttaata attccagcca tttttcgttc attggagaat
    22261 cagccgatag ttctctacgt ttggttgtct gctgatcatt tcgctgggtt aacaagtata
    22321 acctttgcca atagatactt tcgtgatcta agtcgcagca ccactctaaa tatttttcga
    22381 tccccaaaaa gaaatgttgt ttgttttcgc taatgaattt taagatatct tgatggacta
    22441 tgtgagaact gcttttgatc gtatacaaat aagcatcttc caaatcttga aaatatttat
    22501 aaaaagcacc gcgcgacatc cccatcttgt caacgatttc tgagactttg acttggctga
    22561 tgtgctgtga gcaaaatatc tccagtaata ggtcatcgat tctctgtttc tttgcgttcg
    22621 ataaattgaa aaaagtttct tttggcatcc acatctctcc tttatctctg attatacgtg
    22681 gatagccctt cattgtcatg aaggattcat tccttctttt tctcttcttc tgtcaagtac
    22741 ccatcttcca ttcgataaac gcgatcactc cattgaatca tacggacatc atgagtaacc
    22801 ataatagtcg ctttttgttt ctcatgagct tctttaacta gtaatttgac gacttcatat
    22861 gcatgatctg tatccaaact agctgttggt tcgtctgcta agatcaagct tggctcatta
    22921 tataaagcgc gagcaattgc cactctctgt ctttctccac cagaaaggtc tttaggataa
    22981 ctatttttaa gtgactggat atctaaagat tgcagcaagg aatcgatttt tgttgaatct
    23041 tctttttttg aagtaacttg gtcaactagt tcaaattgtt cctttaccgt taaaaaagga
    23101 atcaaattag agctttggag aataaatcct atttctttaa agcgtaaatc tgctcgtttt
    23161 ttttctggaa gctcagttag tgagtgaccg ttgatcacta tttctccgga agtcggtgtt
    23221 tgcaatcctc ctgaaatggt aaggaaagta cttttacctg aaccagaagg accaatgatg
    23281 ctgacgaatt ctccttcctt tacataaaaa tcaattgctt tcaaagctga gacctttgta
    23341 tgaccagaac caaatgtttt ggtaactttt ttcatcgtta atattttttt catttttatt
    23401 aacctcctat tgctgtaatt gggtctactt tggttatcgt acgtatcgaa aacaatccgc
    23461 ctagcattgc gacaatgatc aaaatcccac tatatagcaa ccattgtgac caaaaaacag
    23521 caaaaggcat cgcgcttggt aaaatcagac tagtcaaata agcaaacaaa agagcaagca
    23581 gaacgcctac caccccaaca ataaaggact gtgctactaa agatttagcg atgaagaaat
    23641 ttttgatccc ttgtgctttc attactccaa aaatagcagt cttctgaagt gtcatcacgt
    23701 acatgaaaat accgaccacg gcagcaacca ctaagaacaa aaagtatatc atcgcattta
    23761 aggtcatatt ttgggcagaa tagccaggaa tactttcaat gaatgtccca atagttaata
    23821 actgtagacc cttcgatgct ttttctttcg aaattttagc tgaatccttt attacgatcc
    23881 cattgatcgg ctgttcttct tcagaagcaa acggctgttg tccatacttc aaagcagtcc
    23941 acgtatctag ggacgcatag atgacgggag aaactgtgta ataagtttca ggaaagatac
    24001 cgacaatcgt caaaggatcg tcgtaactgc caattttgat cgtatctcca attttatagc
    24061 ctgaatcagc cagattttga gagatgatga tttcattctt tgttttaaat tcatgccctt
    24121 tcgtgagttt tggtaataaa aatgcttctt tcgttgtgcc gaaaactgta acattttctt
    24181 tgttttttcc tttgattgct ccgctgtaca atccgattgg tgctttctct cctccttcaa
    24241 tgtatttcag atctcctctt gttaattgtg aagctgcaaa tacttgattt gcatcctccg
    24301 acaaaacaat ctcttgtgcg tcccaatcat cgattgcttt tttaaactct tctgaaagtc
    24361 cattcgctag tcctgacaac atgaatacga catacgcaat caagaacata ataccgacga
    24421 tcaagccata tcgtaattta gaatagcgaa tttctttgat agctagaaac atttgttttt
    24481 cctccctatg atagattgat agtgacaaaa tgtcacttgt tgaatagtgt aactctaaag
    24541 tgacaatttg tcacttgtgt tgtttttttc ttatcaataa attgtctaag acatctttcg
    24601 ataaaacctt aacatgagtc ataaaaaatt tatttaagcc ttatttatgc tttccatgat
    24661 aaactaaagt gaccaaatca atgaggtggg aaaatgatta aactattagt agtagaagac
    24721 gataaagtat tatccaataa tataaaagaa atactcgctg caatcggcga gataacacaa
    24781 gtttatgatg gtagtgaagg actgttcgag ttggaaagtg gagtttatga tcttgcaatc
    24841 ttggatttga tgatgccgga aatgaatgga tatgaagtgt tagcaaaaat acggaaagaa
    24901 aaaatacaga ctccagtatt gattttgact gcaaaagata gtttggaaga taagattgaa
    24961 ggatttgaac gaggtgcaga tgattatcta acaaaacctt tctatcgaga agaactgatc
    25021 atgcgcgtga aagcattatt gaaacgctcg ctaggtctct ttgatgaaaa tgttctgaag
    25081 tataaggata tcagttgtga tttaaaaagc aatgaagtct tgtataaaga acaagttttg
    25141 cccatacaag gaaaagagtt tgaattgctc gtttattttt tacaaaataa gggaatcatt
    25201 ttgacaaaag agcagatatt tgaccggatc tggggatttg attctgatac gacgattaca
    25261 gttattgaag tttatatgag caacttacga aaacatctgc gaagtactgg attggataaa
    25321 gaaatcaaaa ccttacgtaa tgtcggttat atactgcaaa gggtgtgatg caatgaatgg
    25381 agatattaca cgaaagcaac gtagtcgctt ttttgcgaca aatgtagcag catttgcttt
    25441 tatctttttt cttttgggat tcattacttt acagatatta aaccaatctg catacagaga
    25501 aacagatctg tcattagaga aaataaattt tgattctcga acagtacaat tagagataca
    25561 aagatatcag caaggcgatc cctttttaaa cccgactcaa acggaacaaa acagaacaaa
    25621 catggaacag ccattaatgg aaccatcaaa tgaccgtttt aacactcaag tgatcttgtg
    25681 gtctaaaaat ggtgagatat tgaataaagc agctattggt gggcgtttca accaaatcaa
    25741 agaattaaaa atggatacga aaaatctgaa taccatccaa aatattgaat taaatgaaga
    25801 cagtggctca tctgccctct ctttccgttc gattacgaaa aaagcggaga cgaatcaaga
    25861 tggcgttgcc tacatccaaa ttttagaaaa tacaaatcaa atcaagaatt cattagcgac
    25921 ttttcgcacg attatgattt tgtgtatgat tgttttttgg attctctcta ttggtatcag
    25981 ttattacttg tctagtctaa gcatgcgccc tattattctt tcttggagaa gacaaaaaga
    26041 atttgttgag aatgcttctc atgaattgcg cacaccgtta acgattattc aaaatagttt
    26101 ggaacatctt tttaccaagc ctaatcatac tattattgaa gaatccgaaa gcatcgctca
    26161 agcattgtca gagacgaggc gcctcactgg attgacttcg gatttgctga caatcgctcg
    26221 gaatgattct gatcagcagc tgctttcaaa gcaaatgatc aatacacaag aatatatcga
    26281 acaacttgtt aaaccatttc aagagatggc catcatagat ggaaaagagt ttattctcca
    26341 taacgatgca acattagaag taccaattga tgaaaaaaaa atccatcaag ttttggttat
    26401 tttgctggat aatgcattaa aatatacgaa tccagaagat aagatcattt tggattcatc
    26461 tgttaaccat aaagaatggc tgattcacgt aaagaatact ggcccttcta tcagtgaaga
    26521 agatcaagca aggatttttg atcgatttta tcgggaagat caatctcggg cgaaagagac
    26581 tggcggatac gggcttggct tggcaattgc aaaacaggta atcgaacagc atcatggaca
    26641 gatttctgta tccaattatc ttccacaagg agtcgatttt aaagtccaat tgccgttgaa
    26701 gcctaaaaaa taatgcttat ccctaaaaaa gggctgtgac aaaaatcttg tcacagccct
    26761 tttttgttgc gaataaacgg tgttccagaa gtagcttctt cggaaataag ccgaaatttc
    26821 tttaagctga ccacttctgt cacaacctct ttagcttacg cttacgattt tttattgcgg
    26881 tcttcaatgt gtacatcacc gcttgtgtca atatccaatt cttctcggct aacatcttct
    26941 gtcacttttt caacatcttc atgttcttct ttatgaatcc caacttcttc acgaataact
    27001 gtatgtttgc ttacatctac ttgctcttct ttgattggga tagtaatcgt gtcatcttcc
    27061 aatttttcgt ctgttccctg tgaagattga tctgttactg gtttgcgttc gatgacgatt
    27121 tcttcacgtt tgaccggtac ttctaccgtt tcagtatcgt tgaccacatg tttgtggatg
    27181 tcgacttctc cagttgtcac atcatgtgta tttacatcta atttttcttc ttttagttga
    27241 attgtatcat catctagatt tggtttgcga tcgatgtctt cagtcttgct tgtacccatt
    27301 gtatcagtct cgccaggtac gtccatcgga tcagttcctg gtgtcatatt caatggatcc
    27361 gttgtatttt gaaagcctgt cgtgccacct acgtccattg gatctgttcc agtgccagtt
    27421 tctaatccag tccccattgt gcttgctggg ctaacagtat cttgtgtatc cattgtatct
    27481 tctgttgttt ctggctgacg atagcctttt actgtgatga caaattttcc atcagaaata
    27541 tctcctctat attgatataa aggatcattt tctggtgtag ccgtctcaga atcataggta
    27601 tcaaaagaaa aagtatcttt tattttttcc cataaagaac ggtcttcttc accatccatc
    27661 cgattttcat ctgtttcaac atctatacca gacaatccgt caaatcctag tctagatgct
    27721 gcttcatcag tagtataaat aacgatatca tctcgttcat acccttcgtt acgcaaccgt
    27781 tcaattactg ataatgcttc ttccctcgta ctatagcttc cgatgaccgt taaatttttc
    27841 tcttccatgt aaattcctcc tagcaaatag atttatgat
//
LOCUS       DANKKG010000023        26330 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000023 DANKKG010000000
VERSION     DANKKG010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.023"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            66..362
                     /locus_tag="PW004_000840"
     CDS             66..362
                     /locus_tag="PW004_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HDL2725830.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            complement(1006..1959)
                     /locus_tag="PW004_000841"
     CDS             complement(1006..1959)
                     /locus_tag="PW004_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2725831.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(1982..2737)
                     /locus_tag="PW004_000842"
     CDS             complement(1982..2737)
                     /locus_tag="PW004_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2725832.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(2734..3693)
                     /locus_tag="PW004_000843"
     CDS             complement(2734..3693)
                     /locus_tag="PW004_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDL2725833.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(3686..4636)
                     /locus_tag="PW004_000844"
     CDS             complement(3686..4636)
                     /locus_tag="PW004_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDL2725834.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            4786..5238
                     /locus_tag="PW004_000845"
     CDS             4786..5238
                     /locus_tag="PW004_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HDL2725835.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(5327..7594)
                     /gene="gshAB"
                     /locus_tag="PW004_000846"
     CDS             complement(5327..7594)
                     /gene="gshAB"
                     /locus_tag="PW004_000846"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HDL2725836.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKKYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(7781..8779)
                     /gene="ftsY"
                     /locus_tag="PW004_000847"
     CDS             complement(7781..8779)
                     /gene="ftsY"
                     /locus_tag="PW004_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HDL2725837.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(8790..9599)
                     /locus_tag="PW004_000848"
     CDS             complement(8790..9599)
                     /locus_tag="PW004_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL2725838.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(9608..13189)
                     /gene="smc"
                     /locus_tag="PW004_000849"
     CDS             complement(9608..13189)
                     /gene="smc"
                     /locus_tag="PW004_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HDL2725839.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(13209..13895)
                     /locus_tag="PW004_000850"
     CDS             complement(13209..13895)
                     /locus_tag="PW004_000850"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HDL2725840.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(14243..16024)
                     /locus_tag="PW004_000851"
     CDS             complement(14243..16024)
                     /locus_tag="PW004_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2725841.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(16050..16964)
                     /locus_tag="PW004_000852"
     CDS             complement(16050..16964)
                     /locus_tag="PW004_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2725842.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(16991..17953)
                     /locus_tag="PW004_000853"
     CDS             complement(16991..17953)
                     /locus_tag="PW004_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2725843.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(17953..18897)
                     /locus_tag="PW004_000854"
     CDS             complement(17953..18897)
                     /locus_tag="PW004_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2725844.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(18898..19902)
                     /locus_tag="PW004_000855"
     CDS             complement(18898..19902)
                     /locus_tag="PW004_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2725845.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            complement(20170..20412)
                     /gene="acpP"
                     /locus_tag="PW004_000856"
     CDS             complement(20170..20412)
                     /gene="acpP"
                     /locus_tag="PW004_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDL2725846.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            complement(20534..21535)
                     /gene="plsX"
                     /locus_tag="PW004_000857"
     CDS             complement(20534..21535)
                     /gene="plsX"
                     /locus_tag="PW004_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HDL2725847.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            complement(21584..23620)
                     /gene="recG"
                     /locus_tag="PW004_000858"
     CDS             complement(21584..23620)
                     /gene="recG"
                     /locus_tag="PW004_000858"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HDL2725848.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            complement(23737..25419)
                     /locus_tag="PW004_000859"
     CDS             complement(23737..25419)
                     /locus_tag="PW004_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HDL2725849.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            complement(25471..25833)
                     /locus_tag="PW004_000860"
     CDS             complement(25471..25833)
                     /locus_tag="PW004_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDL2725850.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            26103..26291
                     /locus_tag="PW004_000861"
     CDS             26103..26291
                     /locus_tag="PW004_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HDL2725851.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
BASE COUNT         7253 a         5722 c         4654 g         8701 t
ORIGIN      
        1 ggattattaa caactcttcg gtagagtaaa cttatcaaat aaaagacttc tttggaggaa
       61 tcactatgaa ggaatatata aaaaacatct attttatcga agaaacacaa aatatcgagg
      121 gttcttatat tgaagtaaaa acactctttg tcaatgaaga taaaaccaaa gcattggata
      181 tttataaaaa attagcttca aaaaaaacga atagttttgg attgatatta agcgagtata
      241 aaatcaaagc agaagagagt tatttctatc aattactaaa acggtggagt aagcttcccg
      301 ccgattttta tcgaaaaatg cagatcatca actatcaacc gcttgctgag acacatgcct
      361 aaatagaggt tgtgaaatca gtgctttgag ctaataccga aggtcagctg attgaacatt
      421 gtttagaaga gattgtgaca actgcgattt gctttgagta ataagaacgc tggacagaaa
      481 atagcttctc atattttcta gtctagtggg cttattgccg agaagcagca gatggaacat
      541 cgtttagaag agattgtgac aactgcgatt tgttttgagt aataagaacg ctggacagaa
      601 aatagcttct catattttct agtctagtgg acttattgcc gagaagcagc agatggaaca
      661 tcgtttaaag aagagatcgg tacaaaataa aaatatctaa atttaagttt tatctaagtt
      721 tcgctttgta cctttgattc aaatcaaagg aggcgatctt gatgctgaaa aaatggatca
      781 cactattttt tgccactccc cattctagaa tcaacccgta tgatgtagat aatgaattca
      841 cgttgaaaaa agatttctca aataaataaa aaccatccgt aaaatcatca atacgatttt
      901 acggatggtt tttgataact tctttgagga taggctcttg ctatttaaga atgtaagaaa
      961 cattattgtt acatagtatg agagtttaat cctctgttat ttatgctatt taaatgcttg
     1021 attcacatct tcgatcatca gtttcattga ttctaaaccg cccccgctta aataccagac
     1081 atctggttca agggagatga tctgctgatt tttaccagca ttcgtttgtg ctaccagttc
     1141 attggcagaa atatcatctt tgctgtcgtc tccaccaata gcttttgttc tgtctactac
     1201 aaataagata tccggatttt tttccagcac atattcataa gaaacacttt gaccgtgagt
     1261 ggatgcttcg atttgatcat ctgcttgttc aaagccaaat aaatcatgga tgaaaccgaa
     1321 tctagaacca gatccgtagg ctgataattg cccttcattc acaagtgtga ctagtgcttt
     1381 tttgtcagta gcttctgctt tctttttcgt ctgatcgatt gcactagata attcctctaa
     1441 ttgagtttta gcttcttctt gcttaccaaa gatctcccct aaagcagtga cattttgctg
     1501 gaaagattcc catggatttt tcgcatctaa agaaaggaaa atggtcggag caatttgact
     1561 tagatctttt tgaaaatctc tttgtcggcc agaaatgatg attagatctg gctgaagctg
     1621 attaattttt tctaagtctg gttcttttat tccacctgct gattctactt ttttatagga
     1681 tgaaagatac tcaggaaggc tgcttgtagc tgctccaact accttatctc caacaccaag
     1741 agcatccatc gtatctaaag aaccattgtc gaatacgacg actttttctg gattatttgg
     1801 cacctcgatt tggtctccat tactatctgt tatcgtcaat gtagtttcag tttgtgtgct
     1861 gctttttttt gtgtctgcct gactggtgtt gttgctattt ccacatgctg ctaaaccaaa
     1921 tgatgctaga atcaagatac ctactactaa ttttttcatc tcttctctcc taatgttagt
     1981 tttagaaata aagacagaat ctgcggcctt caatctcaca aatccgtatg tccatttcgt
     2041 ataattcatc caatacatct ttttttatca tttcttgggt cgttccttgc gtaaataact
     2101 gtccatcttt cattgcaaca atttcgtctg cataacttgc agcaaagttg atatcatgaa
     2161 gaacaataac aattgttttc ccgatttctt gtactaaatt ttttaaaatc tgcatgattt
     2221 gaaccgcaaa attcatatct agattgttca aaggttcatc cagtaaaata taatccgtgt
     2281 cttgcgctag caccattgca atatacgcac gttggagttg accgcctgaa agagtgtcaa
     2341 tcgattgatc cgctatttct tctaacccaa ggtgttccag tgattcctga acgatttcct
     2401 cgtcttttgt cgtcaatttt cctttcgtat aaggaaatcg tccaaattct actaattctt
     2461 tcacggtgat ttggacattt atttcattcg attgtcgtag gatcgataat tttttggcta
     2521 attcattgga tttccaagcc tttacttcgt tatggtctaa atagatttca cctgtatctt
     2581 ttggtatcaa acgactgatc atcgacaaca ccgttgactt tccagcacca tttggaccga
     2641 taaaagcagt caacttcttt tcaggaatcg ttaactgtac atctttaacc actgattttc
     2701 catggtacag tttattgacc ttttgtaaat caatcatagg atactcttcc tttcgcgaat
     2761 caattttgtg acaaaataaa ttcctccgac aaattcaacg acgacactca ctgttgtatt
     2821 ccagccgaaa atctgctcta ctgcgagctg tcctcctgtc agaaacagca tagctaccag
     2881 aaagcttccg ataaatagct gaccatgacg ataagtagaa aagacacgat aactgatatt
     2941 tgcgacaata aaccctaaaa aagtaactgg acccacaaga gccgttgatg tccctgtcaa
     3001 aagactgaca caacataaaa tcaaaaattc aaaacgatct gtgtctatcc cgaggtttac
     3061 tgcttgttca tttccgagat ggaaaacgtc tagcacatgt cgatatcgaa ataacaggta
     3121 ccagcagccg ataatgatca aagaagcagg caaaagatac tgactctgga tattgccaaa
     3181 gcttgcaaat aatctgcctt gaagcaaatc atattcattc ggatccatga tgacttgtaa
     3241 aaaggtactg acactaccaa aaagcgtacc taaaatcatc cctgacatca gtaataaaaa
     3301 aagattcgct ctcatttttt tcaaaagaaa gaaaaataac aatagactca gcccgaccat
     3361 cagcccaacg ttcaataaga aaaaaactgt acctgtctgc tgaagcatcg tcactcctcc
     3421 gataaagaag aaaaggaccg tttggacaag gacatataac gactcaagtc ccaatactga
     3481 aggagtcaaa aactggttat gcgtaattgt ctgaaagctg attgtggcac tgctgacagc
     3541 tagcgagaca aggataaatg caaaaatttt cttactcctc aatgcacatg caaaatgcca
     3601 gtttccatat gtatggatcg tcaagtacat tcctgtaaca aataaaacaa gaacaagcat
     3661 accaataaaa atagatggct tttttttaag catatcgtcg tccccttatc agaagataaa
     3721 ggaagatagc cccaccgatg attccaacga tcacactgac tggtatttca taaggaaaaa
     3781 tcaacacgcg agcgacaaga tcgcaaaata ctaagaacaa actacctgct aaagcagtta
     3841 cgccaattgt tttgtgcact tgatctccat atattctaga gacaagattc ggaatgatca
     3901 ctccgataaa aggaatgctt cctaccatga tcaacatgac tgcactagcc aatgatacta
     3961 agccaatgcc taaaaactgc atcttctggt aatctattcc cagatttact gcaaacgatt
     4021 cgcccagacc aatgatcgta aatcggtaag caaatagcca aaccaagagc aacaatggaa
     4081 caacagcata caataactca taatttcctc gtatcacagt cgcaaaattc ccttgaagcc
     4141 atgaagtcat attttgtacc aactggaatt gataagcaaa aaaagtagca actgaaccaa
     4201 tgatattccc taacatgact ccaatcaacg gaagcatcaa ttgatttttc tggaggaaca
     4261 atcgcgtaag ggataaaaaa agcaatgttc ctgcaaaagc aaagcaaaat gctgtcaagg
     4321 atcggacaat caaggatgca ccaggtagga aaatcatagc cactaaaata cctaaacgcg
     4381 cactatccat cgtgcccgca gtcgttggtg aaacaaattt attttgggtc aaatgttgca
     4441 tgatcaaccc acaaatacta ctggtcgctc ccacaaggat caaacaaatc gttcgtggca
     4501 aacgagtcac tagcaagatt tgtaagcttt gcccatctaa ctggagcagt tctgacaatt
     4561 gtacattcga tactccgatg aacaatgaaa tactacttaa aataactaat aatgctacta
     4621 gtcgtccgta gcgcatccaa tccacctttc taattgagaa tgattttcat tcttatttta
     4681 agtgaatcgt tgaacaaaag caaccgcttt ttcatatgtt ttaggtatta ttgggaatat
     4741 gctataatcg taagaaagac aaagagaaga aggagggtat tttttatggc acaagatgat
     4801 gtaaaaagcc atttggataa gggactttat ggtacacctt tagtgaaccc tgaagaacag
     4861 cataaatatt tagggacatt cagggaacgc tgctatctta gtatgactgt tgctgaaatg
     4921 atccaaccaa aaaacaaaga aaacttttta aaagaactca aaaaacatcc agatgcttcc
     4981 atactcttga atggggctct acccctttct gtccaaaacc aatatatcca gctagcaacg
     5041 aaaaacaata gccgtttcac tgtagtcaac gattacgtca ctgcaaatcc tgaggaattc
     5101 ggattgctct taacagcaaa agaagcagta aatgaacctg tgattgatat cgaaaaaaaa
     5161 tacccagaag aaaactcttc gccagctgaa aaagaaaaac caaaaaagaa aaaaagcttc
     5221 tgggatcgtt tattttaaaa cgaaaaaaca cataaatttt tacatgcaaa aaaagctttt
     5281 gatcagattg acatcaaata ggacaatctg tcaaaagctt tttcttttaa aatacttcag
     5341 gatataacaa tttcaataca ttcatcgtca atcttctgcc ttttcctgca aaaggataaa
     5401 catgcatatg catggctgga ttgaaatttg cttcgatgat cccatatgct tttttatcag
     5461 ttgtaggatc gatttcttta tctggtataa tcaaatcgat accactaatc ttagctccta
     5521 gtgcttctac cgcagaaacg gcgatttttt tgtaagcttc agaaaattca tcagtcatat
     5581 cgatcgaatc accacctgta ctgatatttg agttctccct tagataaaca atttgattgg
     5641 cttggggaac tgattcgatc gtcaaccctt gttctttcaa catcaattgt tctaattcac
     5701 ctaattggat caattctaaa ggtgcacgat gattcgttcc acgcaaaggg tcactatttt
     5761 tttcttcaac aagttctttc actgttcgga tactgtcacc aataacatta gcagggactc
     5821 gcaacatgat ggcttggact tctccgtcga tcacaaagaa acgatattcc gttcccggca
     5881 taaattcttc tactaaaacc gaactgtctt cacgaaaagc aatggctaaa cctgcttgat
     5941 aatcttccag actagctcct tccttgaaaa tcgtgatacc taacccgtaa ttggttgatt
     6001 taggtttgat cacaaatgcc tgctcggcaa atctcggata agcttttacc gcttcttcca
     6061 tggatgaaaa ttctgcgcct cctggtaccc ggaacccagc ttcttttaag acttttttcg
     6121 ttactgtttt gttttccatg ataagtggga cgatatagct gtccttactt gtcatgtttg
     6181 catttttcac atattctaca tggtcttgga attgcagacg caaaaattga tccgactcat
     6241 ctaatacttt gacttggaca cctttttgga tggcatcgaa cataaatatc tgagtagaaa
     6301 gttccatttc gcgaaaaccg gccaactgat agggacgttc atggctttct ttataatatt
     6361 gatgtcccaa tgccacagca acttcttgct gactcgatag ctgtgcttct tgatagagtt
     6421 tcgctgctaa cgtttgttca ggatgatcca tttgagtcaa tgcttcctct actaaataaa
     6481 gagaatccaa ccaatccaat tcaaccagca tttgcttcat tccttcaaga atttctctac
     6541 cttcttgatg aaattctgtt tgatctgatg gttgttctag tgctactttg ttatttcgag
     6601 tcgtaccttc tgctacccat tggtcacagt cttctttttc ttctgtccat aacatgtaca
     6661 tcaggaataa ttgtaagaac cgaacttgct ctggattgat acccaatctt gcataaggat
     6721 tcaaatcgat attccgcaat tcaatataac gaacacctgt tgttgctaaa tctgcgactt
     6781 tctttccgcc tcggaaacga agcggtgtat aaaattcttt ctcctccgaa agtttccctt
     6841 cttcgatcat tcgctcgata tctgctaaat actgtttcat agaagcatag gaaactttca
     6901 catttggatg attcgtatag cccagcgcac tgttgcgaat actgcgaaca ggttcttgcg
     6961 gatgtgattc atctaaaaag tagttcttct cactcattgg tgaagcaccg aataaataag
     7021 tgatcatcca gcgatagcgc ataaagtttc tcgctgtttt caaataaagt tctgttttga
     7081 attcggaaaa atcttgaaat tcttcctgat ggctgaacaa tgttcttagc aactcatcgc
     7141 caaattcaaa attgaaatga ataccgctta ccatctgttt tcgcttcccg tatttttttg
     7201 ctaaataacg tcgatacaag acatcttcga aattttttaa ttttgcaata atgatctctt
     7261 cgtctttttc cggtaaggca ggtggcatac ttaatggcca gatcatctct tctttcggta
     7321 tagaacgagc agtcacatca taaacagcag cgagatactg aaataattcg ggaatagaat
     7381 cggtaacagg ggtgatcatc tctgtttgtg tttcgctaaa atctgtttgg atataggggt
     7441 gataggatcg atcgccaaag attgctggat gatcggtttt tgctagattt cctgcaagat
     7501 caacccgctg tccttcccgt tctataccaa atcgagcttg gtcgataaaa gggcgcgcat
     7561 tgacatgcaa taataattgc ttaaaattca tcattaagtg acctccaatt catatgaatc
     7621 taattataat caaaaagtct aagaaaaaga acgaaattgt ttgagaaccg cttacaaata
     7681 agtataaaaa tgaaggcatc aaaaacacag ctatgttaaa aacaatcatt caaacaagga
     7741 gctgcaactt ttgtcacagc tcctgctttt tagacatggg ttattcttct tttagtaatc
     7801 ctttgaacaa tccaacgaca aaatcatttg gatcaaacgg ctccaagtca tcgattcctt
     7861 caccaagacc gaccaatttc actggtaaat gcaattcatt acggatagca agtacgatac
     7921 cgccctttgc tgtaccatct aattttgtca aaacaagacc ggtcacatct gttgtttctt
     7981 tgaattgttt tgcttgaacc atcgcgtttt gaccagtggt tgcatctaat acaagaagtg
     8041 tttcttgggg tgcttctggt aattcccgct ggattactcg ttttattttt tctaattcgt
     8101 tcatcaagtt cactttattt tgcagtcggc ctgcagtatc aactagtaaa acatctgcct
     8161 gctctgtctt tgctctttcc aaagcatcga atacgacagc tgctggatcg cctcctgcat
     8221 tgccacgaac aacttcaaca ccagcgcgtt cgccccagac aactaactgg tcgattgctc
     8281 cagcgcggaa ggtatctgct gctgccatca atactttttt accttcgttt ttgaactgat
     8341 gggcaagttt accaatactt gttgttttcc ctacaccatt aacaccgaca aacaaaataa
     8401 ctgttagtcc gtcttcttgg atatttagtt catttacttc attgattcct tccgcttcat
     8461 agagatccac aagtttctcg atgatcgcat tttggacttc tgccgatttt ttcgcatttc
     8521 tgagtttcac ttcttgacgt aatgcttcgg tgattcgtat cgctgtttca aaacctacat
     8581 ctgcaccgat aagtgtttct tctaattctt caaaaaagtc ttcatcgact gttcgaaaat
     8641 tggcaaacaa ctcattcatc cgttcaccaa atgttttacg cgtcttttct aaacccttgt
     8701 cgtacttctc ctgtacttca acttcttctg ttttctcttc gccagtaaaa gcacgcttga
     8761 ttttatcaaa aaatcccatt tgactccccc tactctaata caaatttttg gatcactttg
     8821 gctacgccat tttcttcatt gctggctgtc acgaaatctg ctgcttcttt gacgaaaggc
     8881 acagcattct ccatcgctac gcctagtcca gcatattgga tcatcggtaa atcattttcc
     8941 tcatcaccga tcgccatgat ttcttcttgt ttcaaaccaa gatcttttgc caataaagaa
     9001 ataccataag cttttgtaac accttttggc ataaattcca aaagcatact tcttgttttg
     9061 atgatctcat acatttcatg gaattctgct ggtatttctt tgattttagg atctaattct
     9121 tcttggtcat aagcgattac tgccttatta agcacaaatt catctgtaat atcgcttata
     9181 tccgctggtt ggaattgcag cagcttattc aacacattgt acaaggattg cttccttggg
     9241 gcactaggca gctgtaaaac cacattgtca gacaaaacat ctagtggcag atccaattgc
     9301 tgagccagtt tgtataagcg ttggatatct gatacttcca tcactttctt ttccatgatt
     9361 tcaccagtat catttttttg taccaaaccg ccgttgaaag tgatgctgta gtcaccttca
     9421 tctaccagtc ctaattcttg cagataatgc gccatcgcag ctaaggggcg tcctgtacat
     9481 agaacgatct tgaccccttg ctgttttgct tgtgctaatg cttgtttatt ctcatctgaa
     9541 atcagctttt gttcatttaa taatgtaccg tccaaatcga ttgctactaa tttgatcatt
     9601 ttcgttccta cactttcgtt tcgttgattt tcgcctcttc attgatatct tccatccgta
     9661 cagaaacgat ttttgataca ccggattcct gcatcgtcac tccgtacagt acatctgctg
     9721 cgaccatcgt tcctttgcgg tgtgtgacta cgataaattg tgtgtcatct tggaaatcgc
     9781 tcaagtaacg accaaaacgt ttcacgttgg cttcatctaa cgctgcttct acctcatcta
     9841 agatacaaaa cggaacagga cagacacgga taatggaaaa gagcaacgcg atggctgtaa
     9901 gtgctcgttc tcctccagaa agcaaactta gactttgcag ttttttgcca ggaggttgta
     9961 cttcaatctc aatacctgtt ttcagcaaat cagttggttc cgtcagtact aattcagctt
    10021 taccgccgcc aaacatattt ggaaagacaa ttttgaattc ttgacggata gcttcaaata
    10081 cttctttgaa tcttgtccgc acttcatcat ccatctcatc catcgtttca aacaattggt
    10141 ttttcgctgc taacagatca tctcgctgtg ttgctaagaa tgtatgtctt tcactgactt
    10201 gttcgtattg ttcgattgaa tttaagttta ctggtcccag tttttcaatc tgttctttta
    10261 aggatgaaac ctttgttcta ctgtcttcga tatcggttgt ttcttgatag tcttgggaag
    10321 ctttctcaaa tgtcagctga tattctgact gcagataggt caaatgatta tctaaaatca
    10381 tttcagctcg gtctttttgg acttctaatt tggattgttc cgacagtcgt gctttttgct
    10441 cacgattacg ctctgccaag actgcttcaa gcttatcgat ctccttttgc cttctatctc
    10501 gctgctcttt ggcttttgcc aactcggctt ttaatgtttc tcgctgagca gataattcgt
    10561 taatttgttt ttccaaactt tcttctgtga cttcatgatc agaaaagtct gctgtcaatg
    10621 tcgccaactg cttttctatc gcttcttgac gttcggttgc ctctgctttt tggactcggg
    10681 caccgcggag ctggatctgc agatgattaa actgctcttt caagacagca agatctgctt
    10741 gttcttgtgc tttttgactt tgtacttgtg ctcttctggc ttccatctgg tcgctttctt
    10801 gacttaacga tttgatttct tcgtcgattt tttgtcgttg cgattcaaga tctgtttgtt
    10861 tgtctttcaa ttcgttcgtt tgcttttcgt aatcttcgat aaactgctgc agttcacgtg
    10921 tctcaaaagt tgagatttgc ttttcttttt caaaacgttc aagttcattt atgatgtttt
    10981 gtagctgatt tgtcgcttct tgttcttcaa aacgtaattg ttcgcctcgt gtacgcaaaa
    11041 cttcttgttc ttcggctaaa cgagctgttt cttgttgcag ctcctgaact tttttttctt
    11101 gtgcttggag ttgtttatct gcttcttcaa attcactagt caactgtttc aattcttgat
    11161 tttgtacaaa caaactgcct tggtttcctc gtttattcgc tccacctgtc atggatcctc
    11221 cggcattcat cacatcacct tctaaggaaa ccactcgata ttgatagcgg atcgtttgcg
    11281 cgattgcatt ggcactagtt aagtctttgg ctaaaaggat cgttcctagc aggttttgaa
    11341 ccacagtttg gatttgatcg ggataactga cttgttcact ggcaatccct ataaaccctt
    11401 ctacagctgc tgcttgtgtc aatatatgag ccggtaattg tcttggtttg attgtcgtca
    11461 gtggaaggaa tgttgctctt cctcctcttt gctgtttcaa atacgtgatg gcttgccgag
    11521 catctttttc attttcaacg atgacatgct gggctgcccc acctaaagct gcttcgatag
    11581 ctaacgtgaa atctgcagga acatcgatca gttcagccac tgctccgaca atacccgaaa
    11641 gctgctgttt atgctgcaag accaatcgaa ccccttggta aaaaccaaaa tagttttctt
    11701 ggatttcttg aaggcttttc ttacgagcac gtagctgctg gacttgattc attaattgat
    11761 acattttagg ctgttctttt gctaatgctt cttggaactt tttcccgttt tgttggatca
    11821 tttctaactt ctcttttgtt tcagttaaag atgattttag ctgcgcctgc ttttgtgtca
    11881 attcttcttt ttgtaagatc aatcggtcta ctgatgcttc tacttccgat tgcttggcta
    11941 acgtctgctt gctttttgct gtttcttgta tatattgacg ttctagatac ttcagttcgt
    12001 ttcctacagc tgcttgttct tgcatcaaat caacatattg atctcttaat tctgccaata
    12061 attctttcgt tgatttttgg tatttttcta attcttgctg tgtttttatg agatttgctt
    12121 cagctttttg gatctctgtt tctttttctg ctgctgcttt catcaagctt tcttgaagct
    12181 tttcaaaatg tttaaccttt ttttgtgctt ctgctaatga agtttgatat tcttgcgagc
    12241 ttttttgtgt atgctttgtc cgctcctgca aaacatcttt ttgtccttct gtctgcttca
    12301 gtttttccga aagatctaat aatacttgct gatttttttc gatcagacga tcggcttgcg
    12361 cattttcttt acgctgcttc gccaaaatgg attcttgttc ttggatcgac tcagatagtt
    12421 tgcctaattc tagattgaat ttcgcaagct gtgcttgttt gttgtcccaa tctttttttg
    12481 ctgtcttgat ttcagcaacc atcaaagaga catcggtttg agtcagtgtt tcttttagcc
    12541 gtaaaaactc ctttgctgcc tcactctggg cggccaaagg agttaactgt tcttccaatt
    12601 cgtggatgat gtcttggacg cggctgaggt tgtcttctgt ttcaaaaagc ttctgttctg
    12661 ctttcttttt ccgctgcttg tatttcaaga caccagctgc ttcttcaaag atcccacgac
    12721 ggtcttccgg tttactactg aaaatcgctt ctacttttcc ttgagaaatg attgagaacg
    12781 attcttttcc taatccagaa tctaagaaca attcttggat atcttttaat ctgcagcttt
    12841 gtttattgat gaaaaactcg ctttccccag tccgtcgata acggcgggtt acactgatct
    12901 cattgtactc caatggcaga taatgatcac tattgtctaa aatcactgtg acttctgcaa
    12961 tattcagctg cttacgggta tcggaaccgg caaagatgat atccggcatt tttccgcctc
    13021 gcagactttt agcagattgt tctcctaata cccagcggat agcctctgta atattgcttt
    13081 ttccgctgcc atttggtcca accactgctg tgacgctatt ttcaaaatca atgactgtct
    13141 tgtctgcaaa tgatttaaaa ccggctattt caattcgttt caaatacaaa gctttccctc
    13201 acttactttt acagtgctgc caatgcattt tccgccgcag cttgttctgc taattttttc
    13261 gatttccctt gtccagtgcc aatcaattga tcatctacat aaacttcgat ccaaaatact
    13321 cgttcatgag ctggaccttc ttcattgatc aatcggtatt cgattgatac atcacctgat
    13381 ttttgcaaga cttcttgtag ttttgtttta tgatccatct catgtgaaaa agcacctgct
    13441 ttgactttag gaaaaatcac tttttcgatg aatgcatggg tcgtgtcgaa tccctggtca
    13501 agatataacg cgcctaaaaa agcttcgaat aggtcgcaaa gcaatgctgg tcgcgtacgt
    13561 cctcctgaat tctcttctcc cttgcccaac aaaacatatt gatcaaagtg acattctttg
    13621 gcaaatttcg acaagctgtc ttcgcgaacg atcgcagctc gtgtttttgt caattttcct
    13681 tctggcaaat gaggatataa acggaataaa tactgagaaa ccatcagttc taaaacggca
    13741 tctcctaaaa attcaagtcg ctcattatcc gataattgca gattgcgatg ctcattcaca
    13801 taggatgaat gggtaaaagc ttgttcaagc aaatttagat cgtgaaaaac gatgttatag
    13861 ttttctttta gttcttttgt cagctggttg tccattttcc acattccttt tatggatatt
    13921 ttttcgatac ttccttatta tactggtttt ttgtcaaatt tgaaatggtt cggtcaaaag
    13981 gtaagtaaag ctttaataat cctagtgatt gacgagaagt tgtgaaaaat tcgtttagtt
    14041 tcgagtatga agaaaaaatt ttgtgaaaat ggctttccat atttttgcat aaatttgact
    14101 taataccgag aaactggact ttgaacaccg tttattggag aagtcatgaa atcagcgcct
    14161 tgacctgaat attagagaaa taaacagaaa aatatcaaaa aaccgaaaaa ggacatcgcc
    14221 ctttttcggt catttttagt gattatgaac gtgaatctgc tgtaacagaa acatttgccc
    14281 atgggtttgg tacatcataa gaccaatccc agtctttgac acggtcgttg actggcaata
    14341 cttcattacg gaacaacgtt ggaatcgcaa atgcttcttc aaatgcatat tgttgccatt
    14401 tttgatatgc ttctttttgg tactcttcat caaatgcttt gcttgaactg atatcgttga
    14461 tcaattttgt attttcttct gattcataac gagtgtagtt gaacgcagca tttggtcccc
    14521 atagtccact tggatttgga tcagttcctg tattccaagc agcttggtaa acatcaattt
    14581 caggatcatc gttttctaat ttatcataga aagaattgaa ttcgatcaga cgacctgttg
    14641 tgtattggac atccaaacca atttctttcc attgttggat atagtaatct gccaatggtt
    14701 gcgctgtttc cccaccggac atcgaagcaa agttgattgt tagtttattt ccatctttgt
    14761 cttctcttaa accatcgtca tccacatctt tgtagccagc atcatctaat agttttttcg
    14821 ctttgtctag atcttgtgtg aatcctttca tctcatcatc atggtatgac tggaatactg
    14881 gaggaatcaa tgaagtcgca ttgcttctca aacctgcata gaaacgttca cctactgcat
    14941 tattatctaa tgcatagccc attgcttgac gtaatgattt atcagccatt tttgaatctg
    15001 gattgtattc cacactcttt gtatctgcat tccatttacc taacttgaat cctagataag
    15061 tataagaagt ttgttcacgg cctaagtttg tatagccagc aatttcttta taggtatcgt
    15121 aagtatctgt tggcatttgg aaaatcatat catataattt agagttcaat gcttcagttg
    15181 cacttgccat cggtacagat ttcatcgtga tctttttcaa tttaggcgta ccattccagt
    15241 agtgctcgtt tggcaagtat tctactgatt ctcctgcaac gatattactc atgtaataag
    15301 ggccaaaagt cactggattt ttacgtactt gatcgcttga ttccatatct ttgattgcta
    15361 caccttctaa cgtatgtttt ggtgctgcat atgaccagat accaccaccc gattgaagca
    15421 tgcttgggtt tacttcttta tattcgattt caactgtttt gtcgtctatt tttttgattc
    15481 cggagattgt gtcggcactt ccatcatgat attcttccat acctacgatg ttacggaaag
    15541 tgtcgtcata acggatacca gtgtaatctt tattcccaat gatttcgtaa gggaaaatca
    15601 catcgtccgc cgttacatct tcaccatctg accatttgac gttgtctttt actttgattg
    15661 tcgctttttt cgtatcttca tctagatcaa gcgttgcagc tccgtcgtct gtgattctga
    15721 aatcgtcatc gtttgcaaat agcggttcat gagaaggagc cataaaagct gcatcatatg
    15781 aatcttggta aaactcccac tggaacaatc ctttaaactg actgtccgtt gcgaccgcag
    15841 cttctaattc gccgtcttcg attgcatcat cattattttt tgttgcaatt ggaaaagtta
    15901 attcctcttt gttattgccg ttgcctccag agttgtttcc agaggtatct gtacctccgc
    15961 ccccgcatgc tgcaagaacg acagcagaca tcagcgctgc aaatccaaaa atcgttttct
    16021 tcattcctat ttcccccttc gcttctatac tagcctaacc gttggcgcgc atctgcagaa
    16081 cgcttgaatg cttgtccaac ataattgata ctcaacatca aaactaaaat aaggattgat
    16141 gcgggtaacc agatccattg ccgattcact aagacttctc cgttacttgc ataaccgatc
    16201 aatgtaccta aactaggtac atttgccggc aagccgaaac ctaagaaagt caaagttgtc
    16261 tcgataccga tgtttgccgc aaagttcatc gtcaaattag taatgattaa tgaacttaga
    16321 ttcggcatga tttcgcggaa catgatttta aaatcattgg ttcccattgt ttttgaggca
    16381 ctgacataat ctcttcttgc ttcagataat gtcttgcttc ggaataatcg ggctttagct
    16441 acccaataaa atgcactcat gatgaatata aaggaccaga cattgtatct ggaaataatc
    16501 gatacgaaga cgataatgat cagcatgatc ggtagaatca tgataaaatc aacgatccgc
    16561 atcaagatgt tgtcaatgat ccctccgtag tagccagaga tgatccctaa tccaaccccg
    16621 atgatcgaag taaggatcgt gatcgcaaat ccgatcgtaa cagagtttct agcacctatg
    16681 atcaattgtc ctaatacatc acgtccgcct tcatctgctc ccagaatgaa cttttgtcct
    16741 tgtgtcgaaa ctgttcctgg tgcagcgtat ttgtcgaaga tactaacata catcactttg
    16801 tcttgatcgg tcagcatcgc accgataaag atcactaata ataaaaccac tagcaaacct
    16861 aatgaaaaaa tagcaatttt atctttttta aattctcgag caatcattcg tatccccata
    16921 ggaggaatac tttcttgaac tgctgtattg tttttttcac tcatcgatcc attctccttt
    16981 ctgattggtt ttattgaatc cgaatacgcg ggtcaacgat actcatgatg atatctgaaa
    17041 gtaatgtccc cactagcgtc gcgattccta aaatcaatac taaggatgtg atcacggtat
    17101 agtcccgttg gccgatggaa tcaataaata gttttccgac tcccggataa ccaaaaatct
    17161 tttcgataac tacagaacca gcaatcaacg aagtgatttc ataccccatt tgagaagcca
    17221 tcggcaacgc tgcatttcgg aaaatatgtc gactgaatac tttattcgtc ggtacccctt
    17281 tggaacgggc tgttctgaca aaatcgagcg atttcgaatc gattacttca cttcgcagat
    17341 attggattgt gacggcagtc gccaaaaatg cttgggtaat tgatggtaac agaatatatt
    17401 tcaaacggtc tatccattga gtgataaatc caccttgcaa acctagtgtt tgtgatccac
    17461 ttgttgggaa ccagcctaaa cgataaccaa aaacaaatag catgatcaag ccgaagataa
    17521 ataaaggaac agcaaaacta aagaagttgt agattacgac aatcttatcc aagattgaat
    17581 tttgataccg gccagcaagc atccccagag gcacagcaat caaataagta aggacaactg
    17641 tcaagatcga tagatataaa gtgttgatga tccgttcgcc gatcaagtca gcaacagagc
    17701 gtttgtataa gaaactttga ccaaaatcgc cttgcaaagc atttccgatc caacggaagt
    17761 actggacata ccatgggtca ttcaagccag ctgcttgtcg catctgttcg atcactgccg
    17821 gatcctgatt tggattgatc aaaccggtaa atggatcccc cggcatcgct tgggctaata
    17881 agaagatcag aacacttaaa ataataactt gcgggatcat caataaaaca cgtcgcagaa
    17941 tcgttttcca cattagcttg cacctccatc tttcaacgcc actttgtgtg tagttgtgat
    18001 cgtacgcaaa tcgtagactc gaccagtcgc atcatagtat tctttttggt tttggatata
    18061 ctcttgttct actcttcgtc gattttcttt gtggctttcc ctattttcta catcgatttg
    18121 cggaatagct gaaagcagac gtttcgtata gatatgtcgc gggtcattat aaatgtcttc
    18181 tcttgtaccg atttctacaa atcgaccttt gtacataatt gcgatattgt cgcacatgtg
    18241 ttttactaca cctaaatcat gtgaaatgaa taaatagctg aggccgtatt cttgctggat
    18301 tcgtttcatg aaattcaata cttgcgcttg aacagataga tccagagcag atacaggttc
    18361 atcggctacg atcagttttg gattggtagc aacagcacgg gctactccta gacgttgacg
    18421 ttgtccgcct gaaaattcat gaggatattt atatagcgca tcttccggca tcccaacgat
    18481 atctagcagt ccttttacct ttttcttttc ttcttgatcg cttaaccgtt caaaatttcg
    18541 gatgggttca gcaatgatat ccaacactcg ctttttagga ttcaaactgg acattgaatc
    18601 ttggaagatc atctgcacat ccttgttata acccattttt ttgcggttgc ttcgcttcgt
    18661 tacatctttt ccttcataca tgatttgtcc ggctgttacc ttttctaatc ccacaacggc
    18721 ttttcccgta gtagattttc cagagccaga ctctcccact aaaccatatg tctttccttt
    18781 ttcgataata aaatccacac catctacagc atatacatga tccgttactc gattgaagaa
    18841 tccactgcga atagggtaat gaactttcag gtctttgatt gtgattaatt ctgtcatcta
    18901 cacctctcct ttttcgtctc taaagtggaa atgcttatag caggagcaac gtaccaagtg
    18961 gttcggtgcg acttcatgaa ggataggctc ttcttcatga tcgcttgctg gtatccatgg
    19021 aatacgtggt gcaaaccgac aacctttacg aggcatgttt ttcagagaag gaacgacacc
    19081 ttcgatcaca tggagttctg catcatgatt tccttcttgt ggtatagagt tcagtaaaga
    19141 gcgtgtataa ggatgcttag gatgatcaaa cagttctttt acacctgcta cttcaacgaa
    19201 ctgtccgcca tacatcactg ccactcgatc agccatttct gctacgactc ctaagtcatg
    19261 agtgatcaag atgatccctg tttttgtttc ttcctgaagg tctttcagta aatcaagaat
    19321 ctgtgcttgg atggttacat ctagggctgt cgtcggttca tctgcaatga cgattggagg
    19381 tttgcaggcg attgcgattg cgatgatgac acgttgtctc ataccgcctg atagttcatg
    19441 gggatattgg cgtccaactc tcgcaggatt aggaatccct acttgttcta gcaattctag
    19501 tgcacgagct tgacgctgtt ctgcattcat ctttgtgtga taggaaagtc cttctttgat
    19561 ctggtcttcg attctcatga gaggatttaa tgcagacaac ggatcttgga aaatcattcc
    19621 gatatcattt ccgcggattt tattatagag cgtttcgttc aggtcgatta aattcagatc
    19681 gttatataaa atctcacctg taatttttgt gttattagga tcaagtaatc ccataatcgt
    19741 agtagccaag gtacttttcc cgcacccaga ttccccaacg attgctaaaa tctcgttttt
    19801 gtctaaagta aatgaaacgt catctactgc atcgtaatag tcatctttga gtcggaatcc
    19861 tgtatgtagg ttctgaactt ctaataattg gttatcggac aatcgaatac ccctctctgc
    19921 ttcctaaatg gaagtttttc ttaataaaaa aggacattac acagaataaa gcgttttata
    19981 acaaaggtta ttcttatctt tgtgtaatga attattaata attatataga aaatcgcttt
    20041 actttgcaac ataatttctg tttatcgcca gaattatcag aaaaatgact tttacgtgtc
    20101 aaaaagatta aaaaaagaag gataaaaagc tgtatgcagc tttttatcct tctttgcttt
    20161 tatttgaaat tatttaagat ggctgctgat ataatctact gcgccaccga ctgtttcaat
    20221 ttgttcagcg tcttcatctg aaatttccgt accgaattca tcttcaagct ctaaaacgaa
    20281 ttccataata ctaattgaat cagcttttaa atcatctttg atgttcaatt catccgtcac
    20341 tttatcggta tctatctcaa aatgatttga aataattttc gctactttgt taaatacttc
    20401 ttcgcgagtc aactttttca cctccaagaa tcatacactt gtcaattacc tacaccatgg
    20461 ctaattttac tggcattttt caggaatgtc cacctttttt tcaatggaac ggattgcttc
    20521 agatatccaa atactattct tcgctttttt caaattgttc gactaattgt ccaactacat
    20581 ctgtctcaag cattgtgtgg atttggcgga tcgtatagcg aacagcatcg gggcctgttg
    20641 ctccatgagt tttaatgact ggtgctttca agccaaacaa aacggcaccg ccatgtttgg
    20701 aataatccat ttctgctttc aaagaacgta agccgtcttt taaaagcata gcgcccattt
    20761 ttcctttgat cccttcattt agaatagcag attttaatag attcatcata ttcattgctg
    20821 ttccttcaat agatttcaaa acagcgttgc ctgtaaatcc atctgtgacc actacatcag
    20881 ccacaccatt gagtagctca cgagcttcta cgtttccaat aaaattcaag cttgtttcat
    20941 tctgaaggag ttcataggct tttttcgtca cttcactacc ttttgttgct tctgttccgt
    21001 tgtttaagag tgcgacacga ggacgtccga tacctcggac tttacgtgca taaaaagaac
    21061 ctaaaatacc gtattgcaac aaatgttctg gtttgttttc tgcgttagcg ccaagatcca
    21121 gcatatcgaa accggcacct tctttcccta ctactggcaa ggtagacatc aagccagggc
    21181 gttcgatatt tttgatacgt cctacgatga acaaccctgc tgctagtagc gcgcctgtat
    21241 tccctgctga aaatacagca tctgcttcac cgtttttcac tgcttgtgca gcaaggacca
    21301 ttgatgcagt cttttttcgt cgaattgctt ttactggctc atcatcacta ttgatttttt
    21361 cgtctgtatg gataatcgtg atgttttttt catttgttaa gtatttcttg atttctgctt
    21421 cttttccgta aagtaagaat tctacttctg gaaaatcttg ctgcgctaaa gccacgcctt
    21481 ctacaattgc ttgaggtgcg tgatctccgc ccattgcatc tacggcaatt ttcattgttt
    21541 ccactccttt ttccgtcatc agtataaagt atagcacagt ctgctaatcg aaataacgat
    21601 tatcctgatt ttctatttgg aggctttgcg ctaaaggctg atactcagga agcatctgcc
    21661 aatcctttat ctgccaaagg gctgcagcct cttctctggc tacctccaaa acatttccat
    21721 ccgtcaccaa gtcagcaatc gcaaattgag gcagaccaga ttgacggaag ccaaatactt
    21781 caccaggacc acgtaattcc agatcttttt cgcttagtac aaaaccattg gtggtctctg
    21841 tcatgatttt catccgttcc acacctagtt catttttagg attagcaact aagatacagt
    21901 aggaagcatc acttccgcgt ccaacacgtc cacgaagttg atgcagctgt gccaatccaa
    21961 atcgatccgc atccatgatc agcatgacgg tcgcgttcgg cacgtttacc cctacttcaa
    22021 ttacagtagt cgaaactaat atctgcatct ggttgtcttt gaaggcttcc atgattactt
    22081 ctttttcctg attcttcatt ttaccatgta acaagccaac ttcatatttt ggtgcaaata
    22141 gttcacgaag tttttcatag atttccactg cattttttac atctaatgct tctgattctt
    22201 caatcagcgg acagatgaca tacatttgat gtcctcttgc cagttctttt ttcgtccaat
    22261 ccagtacact gtccaattga ggtgttctta cccagcgtgt ttcgatcggg atacgtccag
    22321 ctggaagttc atcaatgata gatacatcca tctcaccata tgctgtaata gctaatgttc
    22381 taggaatcgg agtcgctgtc ataaataaga catctggtcg ccagcctttt tcacgaagta
    22441 ctttccgctg gttcacgcca aaacggtgct gctcatccgt aataactaga cctaaattct
    22501 gaaattcgac tccatcttgg atcaatgcat gtgtgccgat cacgatgtta atttcccctt
    22561 gctctagttt ttcaaggagc tcacgacgtt cttttgcttt cgttgacccc gtaagcaatg
    22621 cgatggttac ttcattggga tcaaacagct gttgcaagct ttccatatgt tgttgtgcca
    22681 agatttctgt aggcaccatc aacgcacctt ggaaacctgc tgtaacggta gcgtataacg
    22741 caatcgcggc aaccactgtt tttccgctac ctacatctcc ctgcaaaagt cgctgcatat
    22801 gctgcggact gcgaagatcc cgacagattt cattcgttac acgtttttgt gcatttgtca
    22861 gttcaaaagg tagcttttga gtaaattctt tcaatttttg gacatcataa aggatctcca
    22921 aaccattcgt ttctgctttt tctgcttttt tcaatccttg aagacgcatt tgaaataaaa
    22981 agaactcttc aaaaacgaca cgtcgtttgg cttgatggtt ttcttcgtgg tcttttggaa
    23041 aatgcatact tcttacagca gaagctcggt caagcagtcg atatttctcc acaaggttgt
    23101 ttggcagatt ttcttcaatc aatgtgccgt attcttgaaa cccttttttg attagatcaa
    23161 ctaatgtcgt ttgacgaatt gatttgttga cgtgatatat cggcgaaaag tcatccattg
    23221 tttgagaacc taaaatcttc atcccgttca atgctttacg ctttgcatcc catttaccgt
    23281 atacggcgat ttcctccgat aaaatgactt tttcttttag ataaggttga ttaaaaaaag
    23341 aaacgttgaa cacatcatgt tcttgcatca tacgaaattg caatcggctt tttttataac
    23401 caaatcgatt catcacagga ggcgagacta cgattccttt caaagtaacc ttttcttgat
    23461 cttggatctc gtttagattt ctttcctgaa tatcttcgta gcggaaagga taataagtca
    23521 gtaagtcttc aatcgtttgg atgcccaagc tggcaagact agctgctctt ttttctccga
    23581 ctcctgccag catagtgatt ggatcatcta aagaacgcat aaatttttcc tccctccctt
    23641 aacaaaaaaa ggacttggtc agcgatcata cctgatcact aaccgaaggc cttttatgaa
    23701 tgaatactgt gttagaaaga tattctgaca cagaaattat tcagcagaaa atagataagg
    23761 atagacaggc tgatctcctt ggtggatctc gatttccaaa tcaggatgtt tttctgttag
    23821 tttctcagca aattcatttg cttgttcaac tgttccttca tcacctgcca agatcgttac
    23881 gatctctgtg tcctcattga tcatttgttc tagtgtactt aacgctgttg ctgtgatatc
    23941 tgggttagag atcaggattt ttccatcgat catgcccaaa aagtctcctt ctttgatctc
    24001 tacaccgtca attgaggtat ctctgatcgc gtgagtgact tgaccgctca caacgctaga
    24061 caacatttct gtcatcgttt ctttgttttc ttctaaagaa gcttgatcat tgaatgaaag
    24121 catagctgtc aatccttggg aaatcgttct tgaaggaaca actgctactg ggatatctgc
    24181 tacttctgct gcttgatcag ctgccatgaa gatgttctta ttgtttggca agacaatcac
    24241 tttatctgcg tttacttctt gaatcgcttt aacaatgtct tccgtacttg gattcattgt
    24301 ttgcccacca ctgataacat aattagcgcc taaactttca aacagctctt ggacgccttt
    24361 accagcagcg atcgctacga taccatacgg gatacgtggt gtttcttttt tcgcaaactc
    24421 gttcacttct tcatcgtgtt ccaaaatcgt ctcatgctgc aagcgcatgt tgtcgacttt
    24481 gattttgact aatgaaccga atttttgtcc ataattcaag acttctcctg ggttttctgt
    24541 atgcacgtgg actttgatga tctcgtcatc attgaccaca agtaaagaat ctcctatacc
    24601 atctaaatag ttacggaatt cttcgtaatc gaatgtacta tcgactgtag gtccttctcc
    24661 gatttttacc atgatttctg tacagtaacc aaaatggata tcttctgtcg ccatttgacc
    24721 ttggacactg cgatgatgtt ctgcattgac catatcatcc atttctgctg gagaaggttc
    24781 atatgtttca tccgcttgga attctccatt caatgcactt aaaaagccct cataaacaaa
    24841 cagcagtcct tgccctccac tgtctacgac accgacttct tttaggactg gaagcaagtc
    24901 tggagtttta gctaaagcac gctttgcccc ttttacaaca gctgtcatta cttcaacgac
    24961 atcatcagta ctttttgctt tcttttctcc agctttcgct gcttctctag ctactgtcaa
    25021 aatcgttcct tctactggtt tcattactgc tttatacgca gtttcaactc catgagtgaa
    25081 ggctgcagca agatcggaag catttaatga gacaacatct ggaatttgct tagaaaatcc
    25141 acgaaacaat tgagacaaaa taacgccgga atttccgcgc gcacccatca gcaatccttt
    25201 tgaaagcatt gctgctaatt ctcctacttt ttctgaggtt gaatctacta ctgccttagc
    25261 tccactagtc atagatagat tcatatttgt tcccgtatcg ccatctggca cagggaatac
    25321 atttaatgaa ttgacgtatt ctgcatttac ttgcagacga ttcgcacctg cctggaccat
    25381 ttcctggaac tggcttgcac ttatttctgt tacactcacc tagataatcc tccttcagat
    25441 cgactgctga gttaccagcc agtctcctgc ctagtctggc agtacacgaa caccttgtac
    25501 aaagacatta actgaattgg cagttacgcc taataatgcc tctagattgt atttcacttt
    25561 ttcttggacg ttgcgggaaa cttcagagat tttcgtacca tagctgataa tcgtataaac
    25621 atctacagca ataccattat cttcttggcg gacaactact cctcttgagt aattttcttt
    25681 acgcaaaatc tcagtaatat tatctttaat ttgatttttg ctggccattc ctacaacacc
    25741 gaacacatct gtcgcggcgc caccaacaac tgttgcaatt acatcattgg taatttcaat
    25801 ggttccagct gacgtttgaa tttttacagc catgaatgag cctccttaac gggtaaaaaa
    25861 ctcccatttt tcttctatca tattttatca tagcgcaact tgaaataaaa ggcagttacc
    25921 caaagtactc aaaaaccttg caaaatttca ggtataacct tgtttaattt acaattattc
    25981 ttctcaattg tcaattgtat ctatgaaaat gtacttgcaa atttccattc tatatgatat
    26041 gttagtctgg tatgagctga attattctat tgctccaaat caaggcaaag gaggaaatta
    26101 ccatggcaaa agtttgttac tttacaggtc gcaagacaaa aagtggaaac aaccgttcac
    26161 acgctatgaa ctcaacaaaa cgtactgtta aacctaactt acaaaaagtt cgcgttatgg
    26221 tcgacggaaa acctaaaaaa gtttgggtgt caactcgtgc tttgaaatca ggaaaagttg
    26281 aacgcgttta attgaatgat acttggaccg ttcagatagt gaacggtttt
//
LOCUS       DANKKG010000024        25545 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000024 DANKKG010000000
VERSION     DANKKG010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25545
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.024"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            72..668
                     /locus_tag="PW004_000862"
     CDS             72..668
                     /locus_tag="PW004_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDL2725852.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
     gene            complement(726..1208)
                     /locus_tag="PW004_000863"
     CDS             complement(726..1208)
                     /locus_tag="PW004_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725853.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1314..1511)
                     /locus_tag="PW004_000864"
     CDS             complement(1314..1511)
                     /locus_tag="PW004_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725854.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            1806..2564
                     /locus_tag="PW004_000865"
     CDS             1806..2564
                     /locus_tag="PW004_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HDL2725855.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            2624..3052
                     /locus_tag="PW004_000866"
     CDS             2624..3052
                     /locus_tag="PW004_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725856.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            3098..3170
                     /locus_tag="PW004_000867"
     tRNA            3098..3170
                     /locus_tag="PW004_000867"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3131..3133,aa:Gln,seq:ctg)
     gene            complement(3320..3691)
                     /locus_tag="PW004_000868"
     CDS             complement(3320..3691)
                     /locus_tag="PW004_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDL2725857.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            3906..4091
                     /locus_tag="PW004_000869"
     CDS             3906..4091
                     /locus_tag="PW004_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725858.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(4174..4362)
                     /locus_tag="PW004_000870"
     CDS             complement(4174..4362)
                     /locus_tag="PW004_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725859.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(4386..4649)
                     /locus_tag="PW004_000871"
     CDS             complement(4386..4649)
                     /locus_tag="PW004_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725860.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            4807..5064
                     /locus_tag="PW004_000872"
     CDS             4807..5064
                     /locus_tag="PW004_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HDL2725861.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(5135..5326)
                     /locus_tag="PW004_000873"
     CDS             complement(5135..5326)
                     /locus_tag="PW004_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725862.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(5467..5787)
                     /locus_tag="PW004_000874"
     CDS             complement(5467..5787)
                     /locus_tag="PW004_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725863.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(5810..6244)
                     /locus_tag="PW004_000875"
     CDS             complement(5810..6244)
                     /locus_tag="PW004_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL2725864.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            6579..7217
                     /locus_tag="PW004_000876"
     CDS             6579..7217
                     /locus_tag="PW004_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL2725865.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPV
                     QAEQPVVQETVQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTAT
                     NGRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(7541..7624)
                     /locus_tag="PW004_000877"
     tRNA            complement(7541..7624)
                     /locus_tag="PW004_000877"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7588..7590),aa:Leu,seq:cag)
     gene            8152..8652
                     /locus_tag="PW004_000878"
     CDS             8152..8652
                     /locus_tag="PW004_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2725866.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(8842..9186)
                     /locus_tag="PW004_000879"
     CDS             complement(8842..9186)
                     /locus_tag="PW004_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HDL2725867.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(9340..10260)
                     /locus_tag="PW004_000880"
     CDS             complement(9340..10260)
                     /locus_tag="PW004_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL2725868.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            10447..10938
                     /locus_tag="PW004_000881"
     CDS             10447..10938
                     /locus_tag="PW004_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HDL2725869.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(10962..13337)
                     /locus_tag="PW004_000882"
     CDS             complement(10962..13337)
                     /locus_tag="PW004_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HDL2725870.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            13663..14289
                     /locus_tag="PW004_000883"
     CDS             13663..14289
                     /locus_tag="PW004_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL2725871.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            14312..15016
                     /locus_tag="PW004_000884"
     CDS             14312..15016
                     /locus_tag="PW004_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDL2725872.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            15208..16041
                     /locus_tag="PW004_000885"
     CDS             15208..16041
                     /locus_tag="PW004_000885"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDL2725873.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            16238..18166
                     /locus_tag="PW004_000886"
     CDS             16238..18166
                     /locus_tag="PW004_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HDL2725874.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            18304..18759
                     /locus_tag="PW004_000887"
     CDS             18304..18759
                     /locus_tag="PW004_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HDL2725875.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            19229..19498
                     /locus_tag="PW004_000888"
     CDS             19229..19498
                     /locus_tag="PW004_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725876.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            19898..20152
                     /locus_tag="PW004_000889"
     CDS             19898..20152
                     /locus_tag="PW004_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HDL2725877.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(20241..21287)
                     /gene="holA"
                     /locus_tag="PW004_000890"
     CDS             complement(20241..21287)
                     /gene="holA"
                     /locus_tag="PW004_000890"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HDL2725878.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(21325..23598)
                     /locus_tag="PW004_000891"
     CDS             complement(21325..23598)
                     /locus_tag="PW004_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HDL2725879.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            complement(23591..24130)
                     /locus_tag="PW004_000892"
     CDS             complement(23591..24130)
                     /locus_tag="PW004_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HDL2725880.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            complement(24266..24484)
                     /locus_tag="PW004_000893"
     CDS             complement(24266..24484)
                     /locus_tag="PW004_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HDL2725881.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            complement(24481..24726)
                     /locus_tag="PW004_000894"
     CDS             complement(24481..24726)
                     /locus_tag="PW004_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDL2725882.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            complement(24842..25519)
                     /locus_tag="PW004_000895"
     CDS             complement(24842..25519)
                     /locus_tag="PW004_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HDL2725883.1"
                     /translation="MIEKISKKILASPKLIIWMSMIVLIFCIASGFLLSNLLFYRETE
                     ETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGSVKEPGIYSFSSEERVYD
                     VLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEHLDQLASSEGKQPTADSE
                     PPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKVEDLQEISGIGEKTVEKL
                     KNFVTIK"
BASE COUNT         7869 a         4972 c         4352 g         8352 t
ORIGIN      
        1 gtaaggtgct atacttcatt tcgtaatcaa ttaaaaaaat gcaaaacgca aatcaaagga
       61 gaaattttat tatgacaact tttactgaca ctttgaaaaa ccgccgttca atctatcatc
      121 taggacgtaa tgtttcactt tctaatgaag aattaacaac tttgatcaaa gaagcaatta
      181 aagaaagccc aactgctttc aatgctcaat caacacgcgc agttatcctt ttcggagatg
      241 cacatgaaaa actttgggaa atcactgaag aagcacttcg cccattgact ccagcagaag
      301 ctttcccaaa tacacaaaac aaattagctg gtttcaaaaa tggttatggt acagtattat
      361 tcttcaaaga tactgacgtg gtaaaaggtc tacaagaaca atttgaatta tatgcagaca
      421 acttcccaga ttggtcagaa caatcaaacg gaatcgcaac agctaatact tgggtagcat
      481 tagttgataa aggattgggc gcaaacttgc aacattacaa cccagttatt gacgaagcag
      541 ttgcaaaaga atggaatatc ccttcaaact ggaaactacg ttcgcaatta gtattcggtt
      601 caccagaaac accggctgag gaaaaagaat acatgaacga tgccgatcgc ttccgcgtat
      661 ttggataatt ttatttgaaa taaaaaacag aggttgtgac aaacatcttg tcgcaacctc
      721 tgtttttatt ctggatcaat cataatatca atctctgcct catcgtttaa gcgttgggac
      781 aaaaatggaa ctaattcttc taatgatgca gaaaatttca gcatcggtcc tcgccatccg
      841 tttcggaaaa aattcatttt tacgatttta gaaccttttg gctgatataa tacaatttta
      901 ttgatctcag aaaaaggaac accttttttg tcaaagctat tcaaaacaag tccttcttct
      961 gtgattcctc tactgtccaa caaaaaacta ataaaaatca ttgctgacaa gatccctttt
     1021 gccaattcat ctgctgtagg atgactgatg agtgtatacc aaatgactag tgacactaag
     1081 aagggtatca ccaagtagac ccatcgattt cgcacagtcc gataaataat ttcttttcga
     1141 accagccaaa gaaataaaaa catcacaaca acgaataaaa acaatagaac cgtaaagaat
     1201 gttaccatga caactcgcct cgctttttct tctccagaaa ataggaaaag aatactgatt
     1261 taccttctca aagaaattgg acttgcagca cgccgcacgt ccaattgttt tctttattca
     1321 aatgtatcat attttgcaac aaccatgctt gctttttcat ctaataattc ttggtcctct
     1381 gcagcacatt gggcaacttt gaccacggcg cagtttgtca tttttgatac aacttcacca
     1441 atgaccgttt ttgtaaaacc aattggtttg catgcatatt gttctcctaa attaatcatt
     1501 tcttccgtca tcaaaatcct cctaaactgc tggaactttt ctccacatta ccactgctat
     1561 ttctttggag atttaactca ttgattttta tttacagtta aaaatcaacc ttacaggcat
     1621 tatagcgtac tgttttcgtt ttcacaatta aaacagctgt tgtatttaga agaatttttt
     1681 tcaaaaaatt aaaacatatt ccttaaaaac taattatttc ctacatatta aaatataata
     1741 cttccatttc caataaaaat acatttataa taggaaagat aaacagctga aaagggagat
     1801 ttcaaatgaa gaaaaaaacg ttcaaaaaac tattgtttct agggggcaca gctgctcttt
     1861 tattaagcgg tacaagttat tacctacata tccattctcc agaacataca atgaacactg
     1921 cacaaaaaag ttcaacacaa aaaacgaaaa agacaaaaga gaaagcagaa gttttactag
     1981 atgttccatt agagagccag tttgattctc cttctttaga aaatggctgt gaagtcactt
     2041 cgctttctat gcttttatct ttttatggtt atgaaacaac taaaaaccag ttagctgagc
     2101 aattggatta cgtccctgtt ttcaatgcag atgggactca tggcgatcct aatgaaggat
     2161 ttgttggtga tatcagtggc ggagactggg ctatggtcgt ctatgttccc ccagttgcct
     2221 cccttgctca gcaaatcgtc caaacggatt atcacacttt tccaaaaacc gatgctcaac
     2281 tcgatgatat caaaaaagca ctatctaaag gaaatcctgt ctggacttct gtgactatca
     2341 attttgaagt cccagatgaa actgatttta tgacttggac cacaaataat ggagaagtaa
     2401 gagtcactcc tcttgttcat gcttgtgtag tcactggata tgacaaagaa aatatttatg
     2461 tcaacgatcc ctatggtgta aaaaatcgag cagtaccgat caacgatttt tcggcgattt
     2521 tcactgcaat gggtggacag atgctaactt tggagaaaag ctagtaattc aaacaaaaat
     2581 cccttatact caagatacca aacctttgaa gggagcgtag ataatggaaa aattcacaca
     2641 taaaaaaatg gatccaaatg aaattccgat tatttttgtc cgtgactgca agggaaatgt
     2701 tcaaggtaaa gtctctatta atgagtggaa cgagcgtcgc agacctgcta ccctcaatga
     2761 actggaaatc aaattgtatc gtcaatctct tgtttattac gcagatcaag agtacgagaa
     2821 agccaccgat ttattgaaat ttctaattgc tcgaacagag tatactcgct ttgaatatat
     2881 tgaacgttta gcaaacatct atcatattat gaatgaacct gtcaaagaat atcagttatt
     2941 ggacaccgtc ttgtcagtag cagaactgat tgcccttcct gctggattag aaaagaaact
     3001 agtacgccgc cttttacgag taaagcaaca actatccgat caagaaaaat aacttgcgtt
     3061 tttttaggaa agtgctatga ttattttgtt gaataaatgg gatgtagcca aactggtaag
     3121 gcacttgact ctgaatcaag caattgtagg ttcgaaccct gccatcccaa tttgatgtgc
     3181 ttgctgaaaa aatgaacccc ctgtaaaaga acaatctttt tgcaggggga ttattgtatt
     3241 atttttacta cttgtattta gtttacataa ttaaattatg aataataaaa tcgggcgtat
     3301 tcttaataca aaaactgatt tacccaataa ttgctaatgc tttctccgga actttttctc
     3361 taggaacttc ctcatacgtt tctatgtgtg ctcctttatt gctaattttc aaataatgat
     3421 tttcttttaa cacagaaaga ccattaaatt caaccgttcg ttctttccca ttggcatcag
     3481 cagctttctg cacatacctt gctgtaccat attcatttac ttcctctggt ttttgtgtct
     3541 ttacatagac ctctcctttt tcaactaatg gatttagctg atcaagtata ccagggattt
     3601 ctccataact tccttcagta taatatttca gtccaaatag caaaatacca ctaatcgccg
     3661 ccagtccaat caaatattta atcactttca tctttctacc tccttgttat atattcagta
     3721 tatgggtcag ttaaagaaat tccatccgtc ccaagttgga gatttttctc ctactttaga
     3781 agcgagaatc gagtgttttt ttagtaaaaa tgatcgttat ttgatattat tgacgtgtgg
     3841 ataaatcagt ttttctcgtt ttgtccatgt aaaaaatcat gttgatagca aaggatgtgt
     3901 tcaaaatgaa tcaagaagaa ttatttcaaa aagtcaagga aatgattaag aacggcaatt
     3961 tcgatggcgc aaaacgattt atcgaagaac ataaagaaca attaggacct tataaagaga
     4021 aagcccaaaa tcttttaaaa gatgtaaata tcgacagtgt caaaaacaaa tttaaaaatc
     4081 tattcaaata agtaaaactt tcacttttgt aaaatcaaag gaggacaatg ctgctgcatc
     4141 gtcctccttt ggttcttttc tgaatacttc atactaaaat aaacgtgtcg gatcgtaatg
     4201 ataagtcaca ttgccgttca gtgaggcttt tcccaccaca tatttacgat tctccaactt
     4261 cagccacgct tctcgaaaag ccattaattc ttctcttttt acatcaatct tttcgatttt
     4321 tccatcgatc aaatcttgaa taagttgttc atatggattc atttttttcc tccatctgct
     4381 gtcaatcaat aagttgtaaa aataggttca tatgcttttt tggtatctag atcttctctg
     4441 actttgactg tcgtataaat aattttttct actgggatac tataataact tccttcacca
     4501 ttattgaatc ggtgaacgtc tttctttgct aaaataccat ttattttatt cgtcagtaca
     4561 cgttgttcga cactgttact tacaacatag taaatcaccc gaatattatt gatcaaatgc
     4621 aaatcgattt ctaacactac atgatccatg aaaatcccac tccttctttc actttgatta
     4681 tagcaagttt ctcctctaat ttcaaaatcg atacagattt taccggataa atccacaaaa
     4741 ctgcttgcgc catttcgcac aaagtgttat tttgtatcat ggactttatt tatcggaggc
     4801 aatagtatga tcgcgatcgg tcaatttgtt ttttatattc ctttttttat tatgataagc
     4861 attcttttct attacataaa atggacaaaa aagaagtttt cagttttact ggcctcttta
     4921 cctgctgtat attttactta tcaaattttt tcttttaggc attgggaaac aacgtctgta
     4981 ttaataacac acatcatcga attgacgctt tcagtaattt ttcttattat ttggatctat
     5041 tttttatata agaatcagaa ttgaatccct cgcatgttta aaaagaaaac gattaaagat
     5101 gcagcaaatt tctgccgatc tttaatcgtt ttttctattc tgttatacca aaaaaatgtt
     5161 tgaatcggtg aaggatcgtt ccttgtttta attctataaa ttcttgtgcg atcgggtact
     5221 tttcttccaa attttctttg ttcttttcga tcaggctttt cgcttcttca tattttttat
     5281 ctgcacatag tgcttttact tcctgtaata cttttcctgc ttccatttgc ctcaccctct
     5341 tgccatttta ttgctttaag ttttatttaa gttatacgca ttattctagt ttcatcccaa
     5401 ataacctttt ttcattttta ctcctcgaac taatcagttt tctaaaggct gattggttcg
     5461 tcttttttat ttttcctcca tttcattctg ctcttctgtt aatttatcta atgctttgat
     5521 tgatgctgcg gctcttgcat aatcatagct gtataattcc ataaatttat caggcagaac
     5581 aaaccacgct ggatgataac cgttagctgt gacagggaat cctttatctt gttggttatc
     5641 tccatcccat ctcagagcaa cacctttttt tcctttccac tttactaaag cgatcgagta
     5701 tcccttcttt gtttgtgtgc cgtcttcaat aattgctaaa atttctattt tttcttttgg
     5761 cgcacggact tcttgaggac gaaccatttt ctattcctcc cttatctcat tacttcacga
     5821 ccaacacact gcaaggggca tgtgatacga cataagaagc ggtcgatcca actaatgctt
     5881 gttgtatagc accttttcct gtcgctccca taacgatcaa atcaatattt tcttgtccag
     5941 ggatgacatt ggcaatcaaa tttcttggat ctccagtttc aactattgcg tggatctcag
     6001 caatgccgta ctctttggca tcattgattt ttttcagcat ggctacttct gttctttgtt
     6061 tttcatctgc atagattttt gaaaaggcat aggcactatg ggtcagctca actttgttga
     6121 tgattgacac gataaataag tgggcttcat tgtcctttgc taagtcaagg gcttcagcga
     6181 acgctttgat cgattgtctt gatccgtcaa ctgctaccat gatgttctgg tatcttttac
     6241 tcattggaac cgcctccttt ttcttttatt gtaacatctc aagcaaaaat acaaaaaata
     6301 tacgcaatat gtaaataaaa tgatgaacaa aagcaatttc tttcaagtta tttatacatt
     6361 tcttttctca tcagaacacc tgttctaatc ccattatttc tgcattatgt gaaattcctc
     6421 atcatttttc acatcgagcg aacatcattc tgttacaaaa aacgtggatt tgtcataaat
     6481 tgtcatgctt cttttaccta tgaaatattt tcacgtgtta aagtattcct cgttgaacac
     6541 aacaaataaa actttttaac gaataaagga gatttttcat gacatcactt aaaactttac
     6601 tttttggaac aactttggct gccggcgctg cattcttcat gggaacgact gctcatgcgg
     6661 acgaagctta taccgttcaa tcaggtgata ctctatcaac gatttctcaa aaatacgttg
     6721 gtgataactc cttgatcaat gccattgcag aatcaaattc aatttctgat attaacctga
     6781 tctactcagg acaacaatta acaatcccaa cagaaggctc tgcacaagca gctgctgaac
     6841 ctcaagcagc cgttcaagaa gcaccagtcc aagctgaacc tgttcaagct gaacaacctg
     6901 ttgtacaaga aacggttcaa acggaaacac aagcagcgcc tgttgctgaa acacaacctg
     6961 ctcctgctgt aacagaaact gctgctactc cagcaagtac aagctctgca aaagaatgga
     7021 tcgcacaaaa agaatcaagc ggttcttaca cagcaacgaa cggacgttac atcggccgtt
     7081 accaattaga ttcttcttac ttaaatggtg actactctgc tgcaaaccaa gaaagagtag
     7141 cagaacaata tgttacttca cgttatggtt cttgggaagc ggcgaaagca ttctgggaag
     7201 caaacggttg gtactaagat tcattatttg aacgtttaaa cacattcaaa atatagtaag
     7261 aggtcatgcg atttattctt tcgcatgacc tctttttgag tttaagttta gtctttattc
     7321 cttaactatc gtttgccagt atttcttgta tcagaatcta taaatctact atttgaatca
     7381 atctattagg ttttaaactg tcccaccttc acacaaattt tttttgattt atttgaatct
     7441 ggttccgttg tttaaaattt ttcctatatt cttttagata atatgttatt ttctttctca
     7501 tcgctccagg tgtaccttct ctttataaaa aaacaaatta tgcggctaag aagactcgaa
     7561 cttccacgag gtcaccctca ctagcgcctg aagctagcgc gtctgccaat tccgccatag
     7621 ccgcatcaca tcttttagtt taggattaca tgtgtttttt gtcaagaaca tatttttgtt
     7681 ctttttcatt tctgtacgcc ctcccttatg ccatctacac aaataattca tccgagcaac
     7741 tccatcatct attgagggtt ggtgaaaaaa tctgttttat ccatatgatc gatttcaatg
     7801 ttaaacgaac gactctggga ttagtaacta ttttattttt ttcttcattt cttttttcct
     7861 ctttttctcg attcagctat cttctttcat ttggatattg attatttctt ctgttgatta
     7921 ctatccttct ggaattctag acgctgaagt aattcacgtt ttctttcttc tagagatcat
     7981 aagtaacgaa tgaacgaact tcaaataatt tttttattgt aattcatatt tgtattgttc
     8041 gatgattctt aatacgatat gacacaataa ataaaaatag gcatggctat ttacaacaat
     8101 agtgctatag tagaaaacaa atgaataagg ttacttaaag aggtgaatac gatgaaactg
     8161 atcaaactat gggaagcaga tttaaagaaa gcttatcagc tacaaactag ctttggacca
     8221 aatgagagtg gatttatcaa caatgcttat ggctatacat ttgctgaatt tttagattat
     8281 gtaaaaaaat gcgaaaaaac gtctttagga atcgatcttc ctgagagtta cgttcccgca
     8341 accgtcttta ttttagaaag tgatgaacat gagtatgtag gaatttttaa tttgcgccat
     8401 aaactgaacg aaacattaag aaacggcgct ggccatattg gttacggcat ctcccctaat
     8461 tatcgtaaaa aaggatatgc cacacaagga ttagcattag tactacaaga ggcaaaaaaa
     8521 atcggcattg atgaggcact tttgtctgta aataaaaaaa atcttggcag tcttaaagca
     8581 caacaaaaaa atggggctgc gatttatcgt gaagatgaga aagaatacta tacgaaaatc
     8641 aaactgatgt aaaaaagaag gattccaaaa ggaaagggat ttgaatgtcc tttatcaata
     8701 gaacacattt atttaattaa cttataatat agcagacaaa attttataaa caaagaaacc
     8761 ttttatctct tttcttctag aaaggataaa aggctttttt gtttttccta tttgcttctc
     8821 atagctagtc agccaagatt tctatcgtat ccccaacgca atccgtgcat aacggctcat
     8881 cttatctgtc gtccatgctg gataccagac tagtttgact tctgctttcg ttacttctgg
     8941 cacttctttt aatgcgtcat ggatagattc tgttaaaata tctgctaatg gacagcccat
     9001 agttgttaga gtcatcttaa tgactgtttc tccattttca ggattgaatt ctatatcata
     9061 aatcaagcct aaattgacga tgtcgattcc taattcagga tcaatcacca tttccaaggc
     9121 agcaagaata cgttctttga ttagttctat ttcttgtcca gaccgttctt ggtttgcttc
     9181 gctcatcgta cgacactcct taacacgatt tttatttatc atactctttt tttagctgct
     9241 tgtaaatgag aatttagaag taagattctc gtttaaattt ataaaaatgt tcttattaga
     9301 tgtaatagat taaatttatt ctaaaataaa cctgattcct catttttttt gtgaacgatt
     9361 ttttatttct ggcaatacgc cagttgttcg atacattttt tgttcgtttt catttgcaaa
     9421 ccggccaaat actgaatgag ttggtaaaat attgataaat gcatcgtcgt cgatttcatg
     9481 aacgatctga tcaagatcat acatttcgta gcgggtgatg accatcatga tcatgttccc
     9541 ttgttttttt gaatagccac cgacagacgg cagtaatgtc attccacgta ccatccgttt
     9601 tgctagttct tctgttacct cttctggctt gacggtaacg atcattgctg ttactttttg
     9661 atgactcgtg tggatggcat caattacttg agtcattgca taaatggaaa taatcgtata
     9721 caaggcactt tcccagttaa agaagaagcc tgcgatgact acaatgatac catttagaat
     9781 cagcatgtag tttccgactg tttttccagt tgtttgtgaa agtacaagag agatgatatc
     9841 catcccacca gttgtaaagc ccatttttaa agataatcct acgccaagac ctaataaaac
     9901 accgcctact agggcattca tcaaaatatt atcagtgatc tgtccgactg gaagcagcat
     9961 cgtcatcacc gatacacata caacattcac aaaactaagg atcgttgctc gttttcctac
    10021 tttgagaaac cctaatacaa acacagggat attcaaaaga aaaataaatg ttcctgtgtc
    10081 tatcttgata tgaaaccatt catataaaag cgaggaaatg atttgagcga tcccattcat
    10141 ccctgcagaa aagacattgg ctggaatcaa aaaataattt aaagcgaccg cagcggtcaa
    10201 tccggttatc aaaataacca ctattttctt taatctttca tttttatgat acgcagtcat
    10261 aattttactc acaagcacct ctcccctact ttggtattcc aaatgtaaga ataacagttt
    10321 atctattaaa ataaaatact tcgaactcaa atgagaagaa ttttagcaaa caggtaagaa
    10381 tctgccctaa tcctatgata agatagatgt atggtaaata tttatatgta tatcaggagg
    10441 gttcctatgg caaaagatgc tagcttcgac attgtatcag aagtcaatac agaagaagta
    10501 aaaaatgcga ttcagcaaac aacaaaagaa ttgtccaatc gctttgactt caaggattct
    10561 acagtagaaa tcaaattaga gaacagcaat ctagttgtat tgagcgatga tgatttcaaa
    10621 atcgaacaga tcaaagatgt attatttggt aagctgatca aaagaaatgt gccaatcaaa
    10681 aatatccatt tttccgaatc agaacacgct ctaggtggca aagctcgaca aactgccgat
    10741 ttggtcaacg gaatcgatcg tgaaaacgcg aagaaaatca ctacagctat caaaaatgaa
    10801 aaactgaaag taaaagcaca aatccaagaa gatcagatta gagtgacagg taagaaccgt
    10861 gatgatctac aagctgtgat tgctttatta cgtaaacttg acttaccaat cgagttgcaa
    10921 ttcactaact atcgttaatc aaaaaacgag gctgacgtct gtcattcctc gtttttttga
    10981 tttactgctt cttttgataa ccaaaaacgc tgtattcctt cttccaattc cccaacactt
    11041 cgtacttctc ttgctgtttg ccaatgttgt gggaataaac gtcgataaga aaaactagaa
    11101 aatcgttgat ctgccaatac aacgattcct tgatccgcca tgccacgaat cactcgacca
    11161 ccagcttgca agactttgtt cattccagga agctgataag cgtaagcaaa tccaaggtgt
    11221 tccttctcat cataatatga tttgatcagt tcctgctcat gattcatctg cggcaaacct
    11281 acacctacga tcatactgcc gatcaatcgg tcccctctca aatcgattcc ttctgaaaaa
    11341 atcccgccaa gtacgcaaaa tccaatcaat gtttcagcgg gatttgtttg aaattttgtt
    11401 aaaaatcgtt cgcgttcttc ctcattcatg tccgtttctt ggattagcac attgctgtcg
    11461 ggatattgct gtctgaatgc ttccaccgca agatctaaat attgataaga aggaaagaaa
    11521 acgaaataat tcccgacctt tccatcagca aaagcatgaa tcgtctctac taattttgcc
    11581 agactgtgtt ccctttttcg ataagtcgtc tctaggtaat ttgcgatgaa aacttgctgg
    11641 tttccttctg ggaaaggact cggcagtttg taagccagac ttttttgtcc ccccagtgtt
    11701 tcctgataat aagagagtgg tgaaaaacta gcagaaaaaa gaatactgct ttttccttta
    11761 tccaacgact gctcaagaaa caagcttgga tcaatacaaa actcttttac tatcagatcg
    11821 cgatacgttt tttcgactgt tgtttcgtag tgatcatcat aaaattcact gaccttcaaa
    11881 aaatgaagca aatcaaaata ataaggaagc aattgttctt gtgatgggtg ctcaggaaat
    11941 tctgccagcc attctttaat tttttcgctt aattgataac ctgcctttac taaagattca
    12001 gccggtgctt tttgatgatg ataatcccaa tgatcttctt ttgctatttc tcggatcgaa
    12061 tcaaattctt ttaatagttt gttgaaacgg cgatgaagtt ttttgaattc ctttggtata
    12121 gcttccttac ttcgtttcgt tttttcataa gatagttctg ccgaatacat ctctcgggaa
    12181 cgattcacca aattatgtgc ttcgtcaatc aaaaacagat agtcctcgtt tttttcatct
    12241 tcaaaaaaac gtcttaaata aacagttgga tcaaataaat aattgtaatc cccaatgatt
    12301 acgtcacacc agatactgac atctaatgat agttcgaaag gacaaagtgt atgctttctt
    12361 gcataagttt cgatgacttc acgagtgatc tgattctcat gattcaaaag atcccataaa
    12421 ccttcattaa tccgattgta ataaccattg gcatatgggc actgatctgg attacaggtc
    12481 gtctcatcta aaaagcagat cttgtctttc gccgtcaaag tgacactttt tacttcagct
    12541 cctgtatctt ttaatgctgt catagcatct tcagctactt gtctagttat tgttttagcc
    12601 gttagataaa agatcttttc tccctcttct tcccctacag ctttcaaagc ggggaataac
    12661 gtagagattg tttttcctgt tccggtaggt gcttctacaa acaatttctg tttcgtacgg
    12721 atcgttttat aagcagctac cgctagttca cgctgacctt tcctatactc atcatggggg
    12781 aatttcaaag ccatcaaaga ggcattgcgt acagtgcgcc agttttcttg aaaaacaagc
    12841 cattctttat aatcttcagt cagcttttta aaaaaagttt ctaactgctc tcttgtctga
    12901 tgttcaacct ttctcgtgat cacttcttct gccgtctgga agtaagtcaa ttgcagattt
    12961 atttcttgta aatcatgttc ttgactatag atataggcat acacccttgc ttgatgaaag
    13021 aaaagttcca cttgttctgg ttctaaatct tcaaatcgtg gttcggaggt tttgatttca
    13081 tctatataat aaataccgtc tcttttgaaa ataccgtctg cacggccttc aacgatcagg
    13141 taatattcat ctaattgaac tgttgttttt aaaaagactt ctttttcata gttttctccc
    13201 gcatcttttt ggagcttgcg gtggattctc gctccttcta atgcagtatg gttacttttt
    13261 ttacgattgt cgatacttcc acgtcgcaaa ataaattcta ctaagtgccg cacagatatt
    13321 tttgactgtt tgatcatttc caccacctct ttctctctaa gattacctga aaaaacctag
    13381 tttagcaaaa agagctctct ttccaaacat aaaaaaacac cctgctgatt aggcagggat
    13441 ataggatata gaatttggat ttttggatga atctaatgta acaaggacat attaaaatgc
    13501 tatgaaaact tactttcctt ctctttagaa tatccaaata cttttcatat agtctgataa
    13561 ttcagataag taaagagaaa taagtacctg tttttgtttc ctagcaatga atgcgttata
    13621 ctaatggatg aaagaagttt tggctatcaa ggaggaatgc acatgcatat cgaaagatta
    13681 aaaacaggaa tgattgaaga aaactgttat ttggtctaca acgaagaagc acttttgatc
    13741 attgaccctg gagaagatgc ggaacgaatc aaacaagaaa tcaaaaaaac aaaccaacag
    13801 ccggtagcga ttttgcttac ccatactcac tacgatcata tcggtgcagt ggaaccttta
    13861 cgcgcgtatt atcaaatccc agtttatgtc agcccactag agcaaaaatg gctaggtgat
    13921 ccgattttga atctttctgg actcggtaga catgatgata ttgctgatgt tattgtctcg
    13981 cctgcagaat atgaatttga aatgaaaact tatactttag gaaacatgtc ttttcgcgtc
    14041 gtccctaccc cagggcattc tataggaagc gtcagcttta tttttgatga ttttgtcgtt
    14101 tctggcgacg ccctgtttag aggaagtatc ggtagaaccg atctgtatac agggaattta
    14161 gaacaattac ttcatagtat tcggacccag ctgtttgtat tacctgatga atttgcagtc
    14221 tatccaggac atggcgatgc aacgacgatt gaacaggaaa aaaggacaaa tccttttttc
    14281 aatcgttaaa acgacttagg aggagaataa aatgacagaa actacacttt atatcgtacg
    14341 acacggaaaa acgatgttca atacaattga acgagtacaa ggctggtgtg atacacccct
    14401 cacaaagcaa ggacaagaag gcattcatta tttaggtcgc gggttaaaaa atgtcgactt
    14461 ttcatttgct tattctagtg acagtggtcg tgcaatcgaa actgcccgta ttattctagg
    14521 agaacattct aagggaaaag aaattcctta ttttattgaa aaaaggatta gagaatggtg
    14581 tttcgggtct cttgaaggtg gatatgacat ggaaatgtgg ggtgtgatcc ctcgtgtatt
    14641 gaattttaaa acatatgatg aaatgtttac cacagaagtt tcttttgaac aaattgctaa
    14701 tgctgtatac aacttagata ccgctgggtg ggcggaaact tatgaaacat tgcgagaccg
    14761 tgtatggaca ggatttgaag atatcgccca tcactgcgaa aaaaatggtg gtggaaatgt
    14821 tttagtcgta tctcatggtt tgacgatcgc ctttttgctt aatctgatcg atcctaaaca
    14881 gcctgttcgt gctgggcttt taaatggcag cgtaacaaag gtcacttatg caaacggtcg
    14941 attttctatc cacggcatca atgatacgtc ttatatcgat aacgggaaaa cacatagtcg
    15001 cgactcgttt ctataatggg gatattttaa tttttaccag agaatcataa aaggcaaagt
    15061 ctgaacgagg gctttgtctt cttttttctc ttcgtgtact tatctgcttt atttctcaag
    15121 tttttcttaa tattaaaaaa cagattgggt ttctcaaaaa ataacggtat actataagat
    15181 gtatcttaat tgggaaggaa ggaatttatg ttttttgcaa atattatcaa agcagtgatt
    15241 ctcggtatta ttgaaggaat aacggaatgg ctgcccatca gtagtactgg tcatttgatc
    15301 ctagcagatg aattcatcaa actggatgcc agttcacaat ttatgtcgat gttcaatgtc
    15361 gttatccaat taggggcgat cttggctgtc gtcgttcttt attttcacaa gttaaaccca
    15421 ttttctccaa gtaaatcaac ggtagaaaaa aaagatacgt ggacactttg gtttaaagtg
    15481 atcgttgctt gcataccagc catcatcatt ggtttgcctt tggatgattg gttagaagca
    15541 aatttccaca agttcttacc agttgccatt gttttgatcg tttacggtat tgcctttatt
    15601 attgtcgaaa aacgcaacaa aaacaaaaca cctcgttgga cttctctgaa tgactttacg
    15661 taccaagcag ctcttcttgt tgggttattc caagtcttgg cacttgttcc cggaacatcc
    15721 cgttctggcg caacgatcct aggatctatc ttgattggtg cttcgcgttt tgtcgctaca
    15781 gagttttcat ttttcttagg gattcctgtc atgtttggcg caagcggctt gaagatcgtc
    15841 aaatatttag ctgaagggaa ttcattcaaa atggaagaaa ctgttgttct tcttgtagga
    15901 acattcgtat cttttgtggt ttccgtctta gctatcaaat tcttactaag ctatttgaag
    15961 aaaaacgact ttaccgcttt tggctggtat cgtattatcc ttggtatcat tttgattggt
    16021 tattggttta ttgcaatgta gaaaaaacca gaacacttct cttatttgag aaatgttctg
    16081 gttttttatt ttctttaata ataatcatta taaataataa aaacaacatc aagcttaatc
    16141 accaaatgat aaccattatc gttctcattt agctgttatt attatcaatt acatttttat
    16201 tatgctatcc ttgttttgac aaggaggtct aataacgatg actaatcaga aaaaagcttt
    16261 tgcagcaaca atcggctgcc ttttatttat gatcttcggt tttatcttcg accgattctc
    16321 tcttaccatc accccacttt tttatatcag tgccattatc atcggtggat ataaacaaac
    16381 atcggaaggc ttgaaagaat tgtggaatga tcggacactg aatgtggatt tattgatggc
    16441 tttagctgct attggtgcct gtttgatcgg taattacttt gaaggagcaa tgttgacatt
    16501 catcttttgt ttaagcggtg cattagagga atacacaact aataaaagtc aaaaagaaat
    16561 tactgctttg atgaatcttc aaccacaaaa agcccaatta ttaaaagaaa atggacaaat
    16621 gcaagaagta gatgtttctc aactaaaggt ggataacttg gtatttgtcg ctaaaggagc
    16681 agctgttccg atagatggct ttttagaaag cacacaggca acgatcgatg agtctgcatt
    16741 aagcggtgaa tctgtgcctg tagaaaaata tttaggcgat gagatgtatg caggaacgct
    16801 gaatcaagga aatcccatca cgattcgagt aacaaaaacg agttccgaaa ctgtctttgc
    16861 taagattatc caattagtag aagaagcgca gaatacgcca actcaaacag cttcatttat
    16921 cgaacgaatc gaaaataatt atgtaaaatt gattttgctt gctgttcctc taatgatcct
    16981 tctcccccac tttttcctag gttggagttg ggatgaaagc ttttatcgag gaatggtatt
    17041 attagttgtg gcttctcctt gtgcgcttgt cgcatctgct acaccagctt ctctagctgc
    17101 tctttcaaat tctgcaaaaa atgggatttt aatcaaaggc ggtatccatt tagaaaagct
    17161 ttctgaatta aaagcgattg cctttgataa aacaggaacg ctcactaaag gaaaaccagt
    17221 tgtaactgac agctttttct ttgatgaaaa ggatttggca caacaacttc ttgttgctat
    17281 ggaacaaaaa acgacgcatc cacttgctca tgcgattttg caatatttcg attgcacaat
    17341 acctgaagaa atccaatact taccaataga agaaattact ggttttggat tacaaactac
    17401 ctatttaggt gcgcaatgga aagttggcaa gcacgcatat gatcctgaga cgatgataat
    17461 cagcaaagag attgcggaaa tgatcgaacg tttggaaaat caaggcaaaa ccgtgattta
    17521 tctttctaaa gatcagcagt taattgctgt tttaggtctg ctagatattc caaaagcaaa
    17581 tacccaacaa gtaatttcct actttaaatc ccaaaacatc catacaagta tgataaccgg
    17641 cgaccattct ggcacagcaa aggccattgc tgaacaagta gggattgaac attactatgc
    17701 aagctgtact ccggaagaaa aaacacaact agtcaaaaaa gagaatgaac tttaccaagt
    17761 aaatgcgatg gttggtgatg gagtcaatga tgcgcctgct ttagcagccg cttctttagg
    17821 tgtcgctatg gggcaaggga cagatgccgc tatggatatc gcagacatcg tcttaatgaa
    17881 aagcgactta aataaatttg tctacagtca tcggctatct ttaaaaatgc ggcggatcat
    17941 caaacaaaat attatttttt cgattcttgt tattctcttg ttgattgctt ctaacttcct
    18001 gcaattttta aatctacctc ttggagttgt aggacacgaa ggaagcacaa tattggttat
    18061 tctgaatggc ttgcgtttac taagaccttt acctgatctt tctgattctt caaaaaaatg
    18121 taagtcttgt ccactataca aagcgcggct gtcttctgtc aaataagcaa agtgtttttt
    18181 tatgattttg tttattttat gaaaaaaaca gcttatgaat aggtcttgaa ggatgaattc
    18241 ctacttttgt aactttctaa aaacatgtta tgattgtttc gaagaaagtg aggaatatat
    18301 ttcatggaaa gttggctttt tttaggaatt attttacttg tagcaatttt agctaaaaat
    18361 caaagtctga tgatagcggc agcttttgtt ttgattatga agctcatacc agcctcccgt
    18421 tcctttttcc cttggcttca aggcaaagga atcaatattg gggtgacgat catcacagcg
    18481 gctattctaa tccctatcgc aacaggagaa atcggtctac gtgatctatt agaagccttt
    18541 aaatccccta ttggctgggt agctgttttt tgcggtgggc tagtggctgt cttatcttcc
    18601 aagggtgtag gattgattgc ttctgatcca caaatcactg ttgcacttgt tttcggcacg
    18661 attttaggtg ttgtcttctt aagaggaatc gccgccggac cggtcattgc ttctggtatc
    18721 acttactgta tcttacaagt atttgcttta ggaaaataga acaaattttg tctattttag
    18781 gagattctta acacccccaa tttactgatc cgtgatatac tgacaacgag gtgatacaat
    18841 gttcgaaaaa ttaaagtatt tcataggaac aatcggctgg tttcttctag cggtactttt
    18901 gattctgggc ttagcttatc tattatttcc tgatttgata caaaatgtgt ttgatactgc
    18961 tttttgattg acctattaaa acgatatagg ggatgctcct gcgatgcagg ggcttttttt
    19021 aatcaatgtt ttgtaataat ttaggccgtg ccaatgagac ttcctataga attcgatgct
    19081 cttttttctt tttggctctt ctgctctttt ttgttatact agattacagt ggacaaaaga
    19141 aagaatgatc gcatccactt caacgaatat caaaatggaa gaattagtgc ctgttttcag
    19201 gttactttga aaagaaagga agacgcttgt ggaaactttt gaaggtcttt taattgatac
    19261 gtacgtttta caagaacagc caatgatgct gactgggatc aacttgcagc ttgaggaccg
    19321 tgtgatcaat ggcctgctta aagatcgcta tttggcaaaa tatatcttac gtttgcccct
    19381 aaataaatat gtgctggaag taagtggcaa atggaaccgt cggaaccagt tggtcataaa
    19441 aaaaatgttg ataaaaaata tcgatgaata cattaaaata atcggtacac cagaatagcc
    19501 tttactgatc aaaggatact ggttataaaa taactgcagt ttcttaatag aaaaacaaag
    19561 aacaacaggg aatcgtatcc cttttcaaaa aaaactttgc aaacggttct ctttgttata
    19621 taataactta gtaccaaaca ttcccattca aactttgttt aaatagataa ataaaaatct
    19681 agtttggttt gacgctattg cctcccacaa tagcgtcttt ttgatttttt tcagcttgta
    19741 ctatccgttt ttactgaaaa aaaatcctaa taaaagactg ttaaggaaaa aaacttgtca
    19801 tgttcatatt gcaaagcacg ctgcctttag gtataataat gaatgttgaa ttatgcggta
    19861 acacgcgtaa gaacttaagt tatcggaggt gaaactaatg ccaaacattg aatctgcaat
    19921 caaacgtgtt cgtacaagcg aaaacgctaa tgtgaaaaat tcatctcaaa caagcgctat
    19981 gcgtactgca atcaaaaaat ttgaagatgc tgtagcttca ggtgctgaca acgtagatgc
    20041 gttatataaa gaagctgtta aagctattga tatggctgaa tcaaaaggtc taatccacaa
    20101 aaacaaagct aaccgcgaca aatctcgttt aagcaaaaaa atcgctaaat aaaagaatcg
    20161 gcttcggccg atttttttat ttgtcaaaaa aaggacaaaa acaaacgacc ttcccgcatg
    20221 ctttgccctc gtctttcttc ttatcgattt cttaacaact gccctgtgcg caaaacgaat
    20281 agttgaaaac ttaattcttt ttcgatttgt cccgttttga tttgataatc catctcaact
    20341 aattgatcaa acaactgtac cagcaaatgt tcatcgaatt tttttacttc ctgcatggca
    20401 agttttacac gatatggatg gattttcaat gtttcagcaa tatttgcctg ctgataacca
    20461 atctttacaa gaaattttgt ctgtaaaaat aaacgtaatt gagaaagcaa aatcgcatta
    20521 attttgattg tttcttcccc ttgcataact aaatcttcgt ataaacgcaa agcttgttcc
    20581 gtcttccctt ttaaaatata ttgggtcatg tcaaaaatat tatgttctaa cgttttagga
    20641 actaattctt ctacttctct taccgtgatt tgctgttgat tttgtccata aaggaccaat
    20701 ttctgtaact ctctcatcat tttggataaa tctaagtcgg tcaacctcaa gaataaatca
    20761 attgcacttc gttccatttt gattgaggaa ttctctaagg tattcatcag atattgacgt
    20821 acttcttttt cattcaactg ttggacattg atgataattg ctgttttttt caattgtttc
    20881 gttacttttt tccgttcatc caacttttca tatggcgcaa agaatactaa tacagtcgat
    20941 tctaatggtt ctttcaaata agccgtcaat aaatcagtat tttgttcgat tccattgttt
    21001 actttttcac ctgttaaaaa ataaggattc tctacgaata ctaaccgttg atctccgaaa
    21061 aacggcaacg tctctgcttc agctactacc tcatctaaac ttgcattttc catgtcaaaa
    21121 cttaaaaaat ttaagtcatc ttcgccctct aattggattt ttttgacaat ttctgtccga
    21181 acttgatctt ggagaaactt ttctgttcct aaaatcaagt aacaagatgc aagtttttct
    21241 ttacgtattt tttgtaactc tgcttgtata ctcaaattac tcaccacttt caaaaattat
    21301 cctaccatga gcagatgaga attactagtc tatcattgtt tccactagcc catgaggatg
    21361 aaaaactgga tgccagcgat aggtgatcat tccttgttga tccgttcgcc atgtttttat
    21421 ctgattttct tctagtgtct ccaatacttc gctgtgcggg tgaccataac gattatttac
    21481 gccgcaggat ataatcccat gttttgcttc aatatgggaa agaaaatctg tgttacttga
    21541 cgtacggcta ccgtgatgac ctaatttgac tacatcggct ttcagattag gataaatcaa
    21601 aagcaattgt ttttccattg cttgattcaa atctcctaaa aataaaaagc gcctattgtt
    21661 atattccatc catagaccaa tcgaatcttc atttcttccc tctccctttt caaaaggagt
    21721 caatacataa agatcataat aaccgctgat cctgtcccca tgtttgattt caatgatctc
    21781 agtaccattt ttttgagctt cagataaatg cctttgtaat ggctcatttc gtccagcgcc
    21841 ccaaccgata taaatttttt tgatagtaaa atgctgagat aacgtgggta attctcccac
    21901 atgatccgta tcgtcgtgtg tcaagatgag tttatcgatt acacttatcc cctgaccttt
    21961 tagatatgga acgatatttc tttcagcagg aggacgtatc ataccttgcg cccacttttc
    22021 tctttcaaag tgtaactgac ctccagtatc gatcaaaatc gtttcttttc gaaaaggact
    22081 ttgcaaaaag atactgtctc cttgaccgac atcgataaaa gcgatcgttg aagaaaagcg
    22141 taaaaatggc aaagtcaaaa aaataaaaga aaaaagacca acataaaaca agtgtttcac
    22201 aggttgttct ttcaacctat ccatacaaat cagtaatccg aaaatataca tcccagatag
    22261 cacaaggggc aaacgtccta taatgagaga cgaccctgaa gaaaaatcga ttattcgctc
    22321 aaataagatt aatccctctt ctatcagcaa aagtaaagtt tccggcagcc agcgattgaa
    22381 gagtgtgaga aataacacac caggaagaaa aagaaacttg aataccggtg caatcagtaa
    22441 tgtaaaaaga ctacctaaaa taggccactc ataaaaatgc catacaatca acggcatcgt
    22501 gatcatagta aacaggacgt gagaggccgt ttttttcaac caatctccat caaaagaaat
    22561 caggataaac aaaaaactca tgaaaaaact aagctgtcca cccgcatgaa aaagaaatcg
    22621 aggatcaaat agtaaaagaa gccaaaagac aactgcaaat aagtccatgg tggaataatg
    22681 cttattcagc aatttgacta gcaatcgcaa taaaaacagc aaactagcac gcaaaacaga
    22741 aatagaactt cctgcaatag aaacataaca gatagttagt agactcaacg gaataatact
    22801 gacgtgtagt ggccatccga tacgtcgaaa caaataatac agccacccta aataaaattg
    22861 gatgtgtaga cccgatagac tgaacagatg cagcaaacca ctctccctaa aaatttcttc
    22921 tggttctctg aatcctgcat ctttatagcc ccataataaa gaattaatat atacacttgt
    22981 tttttgcgga aattcagatt ggacaaagga gataaaagat cctctttttc tctgaagcca
    23041 gtttcgccag tgattttgca tggtgatctg ttcaatcttt tgaatttgga accgtccaga
    23101 aattctatgg ttcctgaagt aagcttcttg gtcaaaatta taaagattcc gcttttttgc
    23161 tggagagatg aactcgcctt cagcagtcaa ttctattccc tttcctcgga agttaagcca
    23221 tgttttcaat tctgcttctg tgtttacttg ataagaaagc tggagctttt gtttattctc
    23281 tgataacctt ccttccattg tcaaaaaatt accatttgca cgaatcgtat ctgtaaatac
    23341 agtaatttgc gcagatgttg ttttttcctc taaaaatgaa tactcttgta gcctataaga
    23401 aatggatacc gttatgctac acaaaataca caagtacgcg ataaaacgtc cttcttttag
    23461 cgaaaaccat aaaaaaattc cggcaagaag caaacgataa ccccaattgt cagtgtaata
    23521 caacaaacag ataatcatcg acgtaattgc cggaaaaatc aaccgatttc gaacatgcct
    23581 ttctaaaacg ctagacatgt tttcctgtta attcattttg aagttttgaa aaatgctgtg
    23641 agtctaaagc aacttggtgt acagatacac ctaattgttc gatcaacgct tgggcataag
    23701 gatcatttcg atagtcatgg agataattga tccctttcac accagcttgc aacaaagcct
    23761 ttgtacaggc caaacatgga aaatgtgtga catagatttc tgaaccttcc gtcgggatac
    23821 caagttttgc acactgtagg agtgcattca tctctgcatg gatcgttcgc aagcagtgtc
    23881 catccacgac gtaacaccct tcatctatgc agtggacatc tccactgact gctccattat
    23941 agccgccagc aatgatcctt ttttctcgaa cgagtgtcgc tcctacttct aaacgtgtac
    24001 acgtactacg taaagataac agtactgcct gagccataaa gtactggtcc caaggaatgc
    24061 gttcattatg tgacttatca gtaacttttt ctttattcgt atcgcatgct tttgaagatt
    24121 tttctaacat gttttatatt cctccgtttt ttcaagttga ttaaaaagta ctcattgctt
    24181 aaacaaaata ttctttaaat ataacgaatc atcctgtctg acaaaagatc ctacccttca
    24241 ctgccgaaat gaagtccgtc atccattagc ccattataaa aattcttcga atccatttgt
    24301 tcaagaaatt ccgtataaaa atcgatactt cacaatcatg gcgttttgct acttctgtga
    24361 gaacagagat atattctttg atcaacttgt tattccgata tttttgtttt gtctcatcga
    24421 ctgctggtgg cgtgataaag aaagcatgat cagaactata gtcttttttc agctgaacta
    24481 tcatccactg cagatttttt ggtacgtttc tttagaaagt tgtttatgct ctgctagatc
    24541 attcgtccca atgagaataa atactttatc tgctgtatag ctaagtacat ctttggcaaa
    24601 gcgttctcgt agagcttgac tgttattgcc gctgacagcc gtattcataa tatgaatccc
    24661 agatatttta ttttggagat agacattcag cattggtttt tttaatgttt ctttgcgaac
    24721 aatcaagcta tttcctatcc aaaaatacta cctcctcgtc ctgccaagct cttagaattg
    24781 gattattctt agtatccatt agcgtccatt tattgctcgt gccagtcgat tttgaatcat
    24841 tctatttaat tgtaacgaag ttttttagtt tttcaactgt cttttcaccg ataccagaaa
    24901 tctcctgcag gtcttccact ttctggaagg atccgttttc ttctctaaat cgaatgattt
    24961 cttgcgcttt gactgatccg atcccaggaa tctgctgaag ttctgatgga gtggctgtat
    25021 tgatatttat tttgggaggc tcggaatctg ctgtaggctg ttttccttcc gaggatgcca
    25081 actgatcaag atgttctggc agctcttcac ctacagcagg gatatatagg acttgctgat
    25141 cttctatctt cgcagaaaag ttgattcgat cgctatccgc ttcttctaat aatccacctg
    25201 cacgctttag tacatcatag acccgttctt ctgaagaaaa ggaatagata ccaggctcct
    25261 tcacacttcc tttgatatcg gtgtacatga cctttggacg agcatcctct tctacatcgt
    25321 ttccttcagc tgatggatct tctccactgg aactaaacga agaaagtatt tcttttgcag
    25381 cagtctcttc tgtttctcta taaaatagca aatttgataa taaaaaacca cttgcgatac
    25441 aaaagataag aactatcata gacatccaaa tgatcaattt gggtgaagct agtattttct
    25501 tgctgatttt ttctatcatt gttctttttt caaagaatga ccagt
//
LOCUS       DANKKG010000025        25517 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000025 DANKKG010000000
VERSION     DANKKG010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.025"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            124..2184
                     /locus_tag="PW004_000896"
     CDS             124..2184
                     /locus_tag="PW004_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HDL2725884.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2267..2836
                     /locus_tag="PW004_000897"
     CDS             2267..2836
                     /locus_tag="PW004_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2725885.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3011..3817)
                     /locus_tag="PW004_000898"
     CDS             complement(3011..3817)
                     /locus_tag="PW004_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725886.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3955..5469
                     /locus_tag="PW004_000899"
     CDS             3955..5469
                     /locus_tag="PW004_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HDL2725887.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5872..6855)
                     /locus_tag="PW004_000900"
     CDS             complement(5872..6855)
                     /locus_tag="PW004_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HDL2725888.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6860..7507)
                     /locus_tag="PW004_000901"
     CDS             complement(6860..7507)
                     /locus_tag="PW004_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL2725889.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7592..8224)
                     /locus_tag="PW004_000902"
     CDS             complement(7592..8224)
                     /locus_tag="PW004_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL2725890.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8240..8791)
                     /gene="coaC"
                     /locus_tag="PW004_000903"
     CDS             complement(8240..8791)
                     /gene="coaC"
                     /locus_tag="PW004_000903"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HDL2725891.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8788..9525)
                     /locus_tag="PW004_000904"
     CDS             complement(8788..9525)
                     /locus_tag="PW004_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HDL2725892.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9522..10511)
                     /locus_tag="PW004_000905"
     CDS             complement(9522..10511)
                     /locus_tag="PW004_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDL2725893.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIITFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10769..11311
                     /locus_tag="PW004_000906"
     CDS             10769..11311
                     /locus_tag="PW004_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2725894.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11328..12413
                     /locus_tag="PW004_000907"
     CDS             11328..12413
                     /locus_tag="PW004_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2725895.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12425..13231
                     /locus_tag="PW004_000908"
     CDS             12425..13231
                     /locus_tag="PW004_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2725896.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13232..14059
                     /locus_tag="PW004_000909"
     CDS             13232..14059
                     /locus_tag="PW004_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2725897.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14056..15129
                     /locus_tag="PW004_000910"
     CDS             14056..15129
                     /locus_tag="PW004_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2725898.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            15557..18253
                     /locus_tag="PW004_000911"
     CDS             15557..18253
                     /locus_tag="PW004_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL2725899.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            18330..18818
                     /locus_tag="PW004_000912"
     CDS             18330..18818
                     /locus_tag="PW004_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2725900.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            18830..19324
                     /locus_tag="PW004_000913"
     CDS             18830..19324
                     /locus_tag="PW004_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2725901.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            19345..20154
                     /locus_tag="PW004_000914"
     CDS             19345..20154
                     /locus_tag="PW004_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2725902.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            20151..20972
                     /locus_tag="PW004_000915"
     CDS             20151..20972
                     /locus_tag="PW004_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL2725903.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            21023..23122
                     /locus_tag="PW004_000916"
     CDS             21023..23122
                     /locus_tag="PW004_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HDL2725904.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            23119..24237
                     /locus_tag="PW004_000917"
     CDS             23119..24237
                     /locus_tag="PW004_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HDL2725905.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            24276..25154
                     /locus_tag="PW004_000918"
     CDS             24276..25154
                     /locus_tag="PW004_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL2725906.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            25300..25497
                     /locus_tag="PW004_000919"
     CDS             25300..25497
                     /locus_tag="PW004_000919"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HDL2725907.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
BASE COUNT         8056 a         4446 c         5407 g         7608 t
ORIGIN      
        1 taaaaaatca aaaaaaatac tctcctaata gtccatcttc ttttgatttt atttatataa
       61 tgaaatcatc ttatgaaaat tactacttca tgtttagaca agagtaggga ggactaagaa
      121 taaatgtgtt caaaaagacg ttcaaaacaa ctaaataaac acgcttgtcg tttagataag
      181 gaacggtcgt gttctgaaaa acactacgtt aagtttatga tgcgcacgat gctgatcagt
      241 ttatctgtag gtgtctatat gcatgtaccc gttcaggcaa cgacacaaac catcaaggtc
      301 gtacgtacct ctgtttctga gaccactgat tcccctatca ctattactca tactttcact
      361 gatgcaaatg gaaaacagct acttcctgca ttgacttcct ataataaagt cagttatcct
      421 ccaacgataa accactactg gttaattcct aacaaaacat tatggacatc ggaaaccgga
      481 agcagtgtgc cgctttctat cttttccgat atgactgatc ctacaaaagg tgatcagtta
      541 aaacagatca ctgatatttt gaatcatgct ttgacagata taaaggacgg tggaagcgca
      601 caagtttctt atgtatatga aaaagattat tcacagtttg aaagaaagaa aatcgatgtt
      661 ctggcaggga caaaagtttc gatatctgat ctggtagaaa ggctgaagaa cgcagatgga
      721 acacaaggag atatgtccaa agtaatttca aacaatggga atatcgtgga tacgactaat
      781 acaggaacat ctactatttc cttgtcctat tttgatccaa ttgctttaaa aacaatgact
      841 gcatctgctg tgatccatgt tcgtgctgat caaacagcta tcaaaggaaa agatcttcac
      901 ttaagaacag gagagagtta cgcccctaaa gatcttgttg aatatgtaac aaaaagcgat
      961 ggaacttcag gaaataaaga agaaatcaaa atgaacggga cagtagatac tacaactccg
     1021 ggagaatatc ccgttgtatt aacttatata gactcgaaaa catcaaatgt ggcagaaaca
     1081 acagctgtag tttatgtaga agcatctgct gcttcatcga ctgtcatcac tcattttata
     1141 gatagtcaaa caaataaaga acttcaagca gaaacacata tgagtagtgt aaatcgtatt
     1201 cctgatttgc gcctacctgc tgaattttct gatcgagtgc caaatataga tttatcgact
     1261 tatgaacaaa atgggaaagt cacttctatt tctgattgga tgcaagcaaa cggattagac
     1321 agaaaaagtc actggtcgga gatccttgaa gtacttcact cagaactgat gaataatagg
     1381 aagcaatcag gaggggaaat tacctttact tatgtttata taccagacaa tcgttcatta
     1441 gaaggtcatg atcttattgt tcatccaaac gaaaaaataa acaaagaaga gttgatccaa
     1501 tcagcaacaa attccttagg taaacccgta ccgttaaagg atgtatcggt tacaatcaat
     1561 gaagtaccat tggaggatgg ttatttgacc aaacaaacag gtgattttac gattatgtat
     1621 agttatcatg acccgtattc actccaagaa ctccatgctc agtcactatt acatgtttta
     1681 ccattaaaca aacagataga tcaaaaagaa caaaaaagac aagaagaaag cttgtcagaa
     1741 aaaaaacaac aaatggctca tgcagtttct ccaacaggga aagtaaaaca aacaagaagc
     1801 ctagagttag caagaatatc tagaaaaatc tcagaatcta ataaaacgat aaggcaagaa
     1861 gctcatttgg ccccacaagt ttctggattg gaaaatgaaa ttttagagcc taaaataaaa
     1921 caacaatcag aaaaagaatt tgttgctaaa cagtcggaag atcagaagga atcagatgac
     1981 cagaaggaaa cgaagaaaaa acgcttgtca actattgatt ccaccgatga gcatctgcag
     2041 ccgcaagtac cacttcctag tccgggaaca cccgcacctg gtggaggtat tccttcttct
     2101 gctttaggag atttcatgcg aggtatggca ggaacatggg tatacgctaa tcgaaatgaa
     2161 tttgacgcag atgactggtt ctgatttata tctctatctg ttatagtcaa ataagtagct
     2221 gagtcatatg ttatttggca taatggagac gaggagggat agaaaaatgt tagataaaac
     2281 gttaccttac gcagaaatct ggatgacaag accacttcaa caaaatgttc caatcacgcc
     2341 tttagcggaa ggttttgaat ttcgatttta tcaaaaagaa gatgaatctg cttgggcgga
     2401 gattgagact tccgttgggg aatttcaaac gacagatgaa gcacttgagt attttcaaca
     2461 aaactttgct ccttatcctg aagaacttga aagaaggatg ttttttgtag agacaaaaaa
     2521 aggagaaaaa gtagcaacct gtaccgcttg gcgaaaaaaa tcaagagagc aagtgtatcc
     2581 ggtttttcat tggttagcag tgaaaccaga ttaccaagga aaagggctcg caaaagcatt
     2641 gactgccaga gtcttgcaag aatttcctac cctccatacg gacggaccaa tctatcttca
     2701 cacacagaca tggagccatc aggcgattgc tctttataaa aaaatgggtt ttacttttat
     2761 tgctgaaaat ctagacggca cacctaatcc agattatgaa aaagttatgc aggttttaag
     2821 taaattagat aaataacgaa atggtgaaat aagtcctcag tattgggata aaaaacgaaa
     2881 tagtcattcc tcatatctta cttttttgag ttaatactat cggataacta atggcatgcc
     2941 atacattcaa aaaagaaggg gcagtgacaa aagtattgtc actgcccctt ctcttgtttt
     3001 ttagcatgtt ttatcgtttg atttttttag gaataaaatt cttgatgaac gtaaattttt
     3061 ggtaatcgtt tcgactatag aggtaaccaa atacactgaa aacaactgct ccaataaaat
     3121 taacaaataa atccttcata gtatccatga ttcccacgtc taagtaacca ttttggatga
     3181 ttgttttatc tgttttacca tctgcatttt gactttcaat gattgtccga gtgatatctg
     3241 gaacatgata gacagtgttt ccgtctttac ttaactccac actattgatt tgctggatga
     3301 tcgtatcttt ttgcatatcg aattgcagta atttatctgc tgaaaattcc acaaactccc
     3361 acataacacc aactgtcatc gaaaaacaga aggaaacaac tgctagtaat aaaggagtca
     3421 agttcatggt ttttgtattt ttattgagca aataaacaag ggaaaagccg acacccccag
     3481 ctaaaaatcc gctgaatgca tgtagaaaag tatcccacat tgaaaaatag ccataaaaat
     3541 cacttaactc tcctaaaact gttgaactaa aaataaaaga taacaaaatg atttctagca
     3601 tatttggaat ctgtatcttt aatatctttt ccagaaaata agggatttca aataaaatta
     3661 atgtcagtaa taaaagcatt gtatttcccc agtgttggtt ccagacctga tccactagca
     3721 taataacgac gatgatacgt aataaaatat agattccgtt tgtgattcta cctgcagttt
     3781 ttgtactgtt cgtaaaaaag gttttcaaat gattcataaa ataactcctt catgtatatc
     3841 cttaattata ctaagaagcg tgtgtttacc aaagctatat gacttaaaga aaaacagagt
     3901 tgatattgtg aaaaaaatca cttaatatag tataaggaga aaagggggat acaaatgaaa
     3961 aaattagttg ctggtttatt aacagccgtt gcacttcttg ctatgagcgc atgcggcgca
     4021 gatcagaaaa atacagcgca gacaagttct agtacgaagg aatctgccga agtatcatca
     4081 ggcgcttctg aacaagtata tacagatcca aaagaaatga aagaatctta tgatgtcgtg
     4141 attattggag ctggtggtgc agggatggca gcagctatcg aagcaaaaga tgctgggttg
     4201 aatccagtaa ttttggaaaa gatgcctgtt gccggaggaa acacatctaa atcatcaagc
     4261 ggtatgaatg cttcagaaac aaaatttcaa aaagaagaag gaatcaaaga ctcgaatgat
     4321 gccttctatg aagaaacgct aaaaggtggt ggaggaacga atgataaaga aatgcttcga
     4381 tactttgtcg atcactctgc tgatgcggtc gattggctag atcaaaacgg tatcaaactg
     4441 gacaacttaa cgatcactgg agggatgagt gaaaagagga cacatcgtcc gtctgatggc
     4501 tcggctattg gtggatactt agtggagggg ttattgaata acgtccatga gcgggaaatc
     4561 cctatttttg tcaacgcaga tgtgacagac attaagaaaa atggggaaac ggtcaatgaa
     4621 gtgactgtac atgttcaagg agaagaacca aaagaggtca ccggaaaagc aatcgtagtt
     4681 accacaggcg gttttggtgc cagcaaagaa tttattgaaa aatatcgtcc cgatttagct
     4741 gattatatta cgacaaatca agaaggatca actggagatg gtatcaccat gattgaaaaa
     4801 ttaggtggac aaaccgttga tatggataaa atacaaattc atccaactgt gcagcaggat
     4861 gagggggtct tgattggtga ggttgttcgc ggagaaggag caatcttggt cgatcaagag
     4921 gggaaaagat ttgtcaatga aatggataca agggataaag tatctgcagc aattacagca
     4981 ttgcctgaaa aatcagctta tttgattttt gaccaaggcg tgcgtgatcg tgccacagct
     5041 attgaatttt atgaccaaaa aggatatgtc acaactggaa agacaatgga agaattagca
     5101 gaaaaaatcg atttaccaaa agaaacacta gcaaaaacag ttgatggatg gaatacggat
     5161 gttgcggaga aaaaagatgc tcaattcaac cgtgccacag ccatggaaca tgacttatcg
     5221 tctccgaatt attatgcgat caaaatcgca ccaggaatcc attacacgat gggcggtgtg
     5281 aaaatcaata cgaacactga agtattagat aaagaaaacc agccaattag ggggttatat
     5341 gctgctggtg aagtcacagg tggcttacat ggaaacaacc ggatcggtgg aaattcagtt
     5401 gcggagatca ttatttttgg gcgacaagcg ggcatacaag cagctcgatt tgcttcggaa
     5461 aatgaatgaa atagaacgta aataaaaaga ggttgtgaaa tcagcgtttt gagctaatac
     5521 cgagggtcag ctgattgaac actgtttagt gaagaggttg tgaaaaagct gccctgcatc
     5581 aagtaataag aacagccaag taaaaacagc ttcttatgtt tttaacttgg ttggacttat
     5641 taccgcagat gcaagctttt gaacaccgtt tattcggaaa aagagcgtgg gacaaaagta
     5701 aagaacactt ttgtctcacg ctctaaacac gtataaacgg cggaagcaga agaaattcct
     5761 tcggaaataa gctgaaattc acaaaaattt gaaaagcaat tttcgtgaat ttcttcttat
     5821 ttcttggagt taaacgcttc tgtcccagcc tcttttttga tcagcatgat tttattcagt
     5881 tatcttcaaa atgatttttc cgatgttttt gtttgcttcc atatattcat gcgcttgttt
     5941 gactgcgctg aattggaatg tacggtcaat gattggccga atcttgccat tttctagaaa
     6001 aggcccagct ttttgcataa aagtttgtgc gagctctgct ttatactgat cacttctagg
     6061 tgtcaataat gtaccaagca gttggacacg tttcccaatc aatacaccaa ggtctatctc
     6121 aggcacgatg gtcccaccta gcataccaat caagacccaa cgtccatcaa cggctataga
     6181 agctaaattc ttttcccagt aagaagcgcc gatgaaatcc aaaataacat cgactccccg
     6241 accttcagtg atttttcgta cctcttcttg aaagtcttgt tttttatagt tgatcaattc
     6301 atctgcacca agttctttac aaaaggcgag tttttcttct gatcctgcag taacgatgat
     6361 tttcgcgtga gaaagctgtc tagctaattg gatcgctgct gttcccacac cacttgctcc
     6421 tgcatggatc aagacagttt cgccttcttg taattttcca agccagtaaa gtgtttgata
     6481 agcagtcaaa aaaacttctg agattccagc agcagaaaca tagtctagcg tatcagggag
     6541 aaggattgca cgatcggcag gcatcacagc atattcggca taaccaccta gattgactaa
     6601 tccgtatact tttgtcccag gagtaaaagt ggagatatca cttttattct caactacctc
     6661 tccagctact tcgactccca aaatagggta gggaggtttc aaagagactg attctctttt
     6721 cataatatct gttcgattga cagctgctgt atggactttg atcagtaatt cgccttgttt
     6781 tggtttagga acaggcactt caatttgttc taatacatct acagaaccag gatgcttgat
     6841 tgaaatggca cgcatggatc tattcctcct ttgcccattc ttggaatgct gtattcaact
     6901 ctttgctacc ttcagaaagt actagagtcg tacgagaaat atccgtttct tccactagag
     6961 cggcaagtac agcagcaact gtagagcgag tgatcgtttt tgatgccttc tcattaggct
     7021 tagttaattg aatggaagta acttcttcat cgttagtcaa tgtgactggt tgaagaatga
     7081 caaaatctaa gttagttcgt ttcagccatt catctgcata atgtttagta atataataat
     7141 ctgtcatgga ctcaggccat tttgctggtt catctgcata aacagcactg accatcagat
     7201 agcgagagac atgcgcattt tcagctgcga tcactgtttt gattgcgcca tccaagtcta
     7261 cttgcagaag attctttcct tgagagccgg cagcaaaaat caatacatcg atgcctttga
     7321 atgcttcggt cattttttcc acggaccaag ttaaatcaaa agagacatac gaaacattct
     7381 ctttttttac taatgtttgg gaagcaacat ctctcacccc tgcaaaaatt tcatgggaac
     7441 ttggagctaa atcttttatc aggtgtcggc caatttgtcc gtttgcacct acaacaaaaa
     7501 ttttcatttg gatcatcctt tcgacgtcaa atctttccat tcaatagtaa caccaaaaga
     7561 gaaaaaaatc agagatcggc tacgatgact atcagttagt aagagaaggg tgcttgaagt
     7621 atcttaacaa gacattgcat actgctgcag tcgcaagacc tgcgacgatt ccttcaggta
     7681 taccacttgt aaaaataacc gctagaatag ctgaataaac agcatttaaa tttgttccga
     7741 tgactttggc ataatcttgc tggaataaaa agtagatcat attcatcact aaaatcgtat
     7801 tggtcaatga accggaaagt ccagctaaga ataaagaaac ggaatctgct ttttgtttca
     7861 ttagtttgcg cagaccttta taaacgaaat aaggaaccac cccaatcaag atgcgaggaa
     7921 taaacgcgat cagcaaggct ttccagcttc catgttctgt tccgatgact gggataaacg
     7981 gagaaaagac aaaagacaaa ggtgtttgga taactgtgct acggatcatg ctgatcagtc
     8041 caaatgtacc gccaagaaaa gcacctagtc ttggtcctaa aacgatcgaa gcaatgatta
     8101 caggaatatg catggttgtt gcattgattg gaccgatagg gataaaacct aaaggcgtaa
     8161 cagctaataa aatcaaaata gctaaaaaca tcgcagttaa tgtaaagttt tttgtatttt
     8221 tcataagtac tccttttttc taagcatttc tttcattaag agtcagcatg acctgttcga
     8281 tgatcgtctg aatatcagct aaggcacctt ttccaaaatc tccacaagct aaaagagatt
     8341 ctcttggttc aatttcctga tagccgacag atctcaatgt ttccagatta cgctgagtga
     8401 tcgggttttg atacatattc gtgttcatgg caggggcaat caattttggt gttcccactg
     8461 gaagcgccat tgcgactgtt gaaagaagat cgtctgcaat gccgtttgct agcttgccga
     8521 taatgtttgc tgtagcaggc gcgatgagaa acaagtcagc ttgttttgct agttcgatat
     8581 gattgattac ctgcggattt gattcttgca tgacgtctgt atggactgcg cgcttcgaca
     8641 gagattgcaa ggttaatggc gtgataaaat gtgtgctgct tttagacata atcacatcta
     8701 cctgggcacc taatttgacg aattgacttg taagatcagc gcttttgtaa gcagaaatac
     8761 ttccagtaac gcctagtaag atctttttca ttttgattcc tccagtctaa ttttctcagt
     8821 aatcaatgca gcaatctcag atttactttg agcttcttcc actgttccat ctttagataa
     8881 gagatagccg tggtgtgtct cgccatggat ttccgtcaaa tcatttgcga aaataaaatc
     8941 agttttgtta tttatcagac tattttttgc gactgccaga agagattcct ttggtacatc
     9001 tactaatagt ttgaaaccaa tgatttttgc ttttggctgc cattcacgta gataagaaat
     9061 gatttttggt gttttcttca aaacgagcac aagatgctcg gtgttagaag agatctttga
     9121 aacagtggta tctgtctgtt cattttccgc aaaccaattg tccagttctt cttgcgttga
     9181 agaagtgggt aggttttttg ccagctgttc ttctgaaaaa ctaaaagcag gagtaaaatc
     9241 actgactgcc atactgtgga taatcgcatc gtagtgttct tttttcgtta gttcttctaa
     9301 ttgtaaaaat aagtccagtg cagattcgat cgtataaatc gttatattgg agtgtctttt
     9361 cggtttcagt gcttgtttcg tagtcacgta atcgattgtg gtatccggat tggcagctaa
     9421 ataatcagct aatgcttgcc ctaatcgtcc tgtcgaatga tttgtaatag cacgtacttg
     9481 atcgattttt tcagtcgttc ctccagctgt gattagaatc ttcatttatg gtcacctaca
     9541 tttactagtg ttcttccttg gtgtgttcca gcaagtagtt tagatgctgt ttcaggcaat
     9601 tgatccagag taatctcttg gacgaccagt tcatctgcaa tctgcaaatc cgccatctgt
     9661 tgccataaag aaagacgttg atctattggt acattgacag aatcaattcc aatcaactgg
     9721 atgtttcgca gaataaaagg caagacagtc gcgtttagtt tgattccccc agcattgcca
     9781 cataaagcta ctgcaccgtt ataagagatc aatggaagaa gactgcttag ctgctcgcca
     9841 cctactgtgt cgatgacata gtcgatttgc tgttttccta aagcttttgt tgtctctggc
     9901 agaaattcat caggtcgtac gacttgcaaa gcacctagac tttttagcca tgtgacagac
     9961 tcttcttttc tggaaaaggt aatgattgac tggaatccta gtttatgtaa cagggctatc
    10021 gctgtactgc ctacaccgcc agaagcacca gtaacgacaa cttttttatc ggaggtcata
    10081 ccttgacgga ttaaagcgtt cacacacaac atagcagtaa accctgctgt accaaggatc
    10141 atcgcttgac gcagcgtcat attttttggt agaggaacta accagtctcc tggtactttt
    10201 tgatattggc tataaccgcc tggatgggtg acgccaagcc caaaaccagt agcaagtaca
    10261 agatcttctt ttgaaaaacg tggattgtta ctttggacga cagttccgga taaatcaatc
    10321 cctggagtca tcggatatgt tcgtatcacc tgaccagact cgctcattgc caatgcatct
    10381 ttataattga catcagaata ggcaattttt accaagactt cattttcagc caactcagta
    10441 aatggcagat cactgattgc tgtatggaaa acaggttcct ttattacctg aaaagatcga
    10501 aaagcgttca ttcgtgagac actcctttta aaattttctt ttatcatacc ataaagccta
    10561 ctcttttatt cttgttcttc cgtaaattta gacaaaaatt aggtgaaaaa taaagaatca
    10621 aacaactaga atttattcca aaagataaag aaatattcag caaaaaaaat tttacaaagg
    10681 gttgcctttt tcaataaatt tcgtatacta atacgagtgg tagtaaacag gaagtttact
    10741 attagaaaac ttcgattcgg aggaaattat ggaaattggt gagaagttaa gaaatttgcg
    10801 catccaaaag aatttgacac aagaagaact aggcgaacgc acagatttgt caaaagggta
    10861 tatttcccag ttggaacgag atttaagttc accatcaatg gaaacattct tttctattct
    10921 tgaagtctta ggtgtgactc cagaagaatt ttttcatcaa gaagcagcga atctgcaagt
    10981 ggtttattca aaagaagatc atacggttta tttcgatgaa gaaaacgggt atgaattgga
    11041 atggctcgtg gctgattcga atgaaaaaga aatggagcct gtcttgctga cttttgaagg
    11101 tgcaggagaa tataagacgt ttgaaccttc attatctgaa acgtttattt ttgtattaga
    11161 aggggaatta gagttaagct taggagagac ggtctatgtg gcaaaagcag gacagtcgat
    11221 ctattatcaa gcgacaaaac aacatcaatt aaggaatcat ctcaaaaaac gaacaaaagt
    11281 attgattgtt gcaacgcaat catatttgta atataggggg taaaaacgtg gggaaaccaa
    11341 tcatttcatt tgatcaagta gtgaaacgct atgatgatga aacagtatta aaaaaagtaa
    11401 gttttgagat cgaacaagga aaattttata cactgcttgg tccttccggc tgtggtaaaa
    11461 caacgatcct acgtattata gctggattca ccgaagctac agaaggggac atctattttg
    11521 aagggaaacg gttgaatgat ttgccagcta ataagcgaca agtaaataca gtcttccaag
    11581 attatgcact ttttcctcat atgaatgttt ttgataacgt tgcttttgga ttgaagatca
    11641 agaaaatgtc caaatcagat atcgaaaaga aggtaaaaga tgctttgcgt atggtccagc
    11701 ttcctggata cgaaaaacgg gagattagtg aaatgtctgg tggtcaaaga cagcgtgtag
    11761 cgattgcacg agcaattgta aatgaaccga aagtattgct attagatgaa ccgttatcgg
    11821 cgcttgattt gaaactacgt acagatatgc agtatgaact gcgggatctg caacaacgat
    11881 taggaatcac gtttattttt gttactcatg accaagaaga agcgttggca atgagtgatg
    11941 agatttttgt catgaataaa gggaaaatcg tccaaagtgg tacacccgta gatatttatg
    12001 atgagccaat caatcatttc gttgctgatt ttgttgggga gagcaatatc gtggacggtg
    12061 taatgatcga agataatcta gttgagtttg ttggtaagca atttgaatgt gtcgatggcg
    12121 ggatgcgtct aaatgaacca gttgaagttg ttttacgccc agaggatttg acgatcacga
    12181 caccagataa aggaaaacta gtcgtaacgg tcgatactca gctgtttcgt ggtgtccatt
    12241 atgaaatcat ctgttatgat gaacagcaaa atgaatggat ggtccattca acgaaaaaag
    12301 ctaaagaagg aagcaaagta ggattggcct ttgagccaga agatatccat gtcatgcgtt
    12361 tcaacgagtc tgaagaagaa ttcgatgcac gtcttgacag ctacgaagaa taggggggac
    12421 aagaatgaaa acaatgcgca ggatctattc cgtcccttat attttgtggc tcggtttatt
    12481 cgtgatcgcg cctgtattaa tgatcattta tcaatctttt tttgatatga atgggcaatt
    12541 cacgctgaat aattatctat cttattttac gtcggggaca tcgctttcta tgacgatcaa
    12601 ttctgtatgg tatgcatttt tgatcacgtt tttcacgctg atcatcagtt acccgactgc
    12661 ttatttttta acaaaactga agcacaagca attatggctg atgctgatta ttttgcctac
    12721 atgggtgaat ctgctgctta aagcttacgc atttattggg attttcagtg tgcacggcag
    12781 cgtgaatcat ttctttgatt ttatcgggat cgggccgaaa cagatattat tcacagactt
    12841 cagttttctg ttcgtggcaa cgtatatcga gattccattt atgattatgc caattttcaa
    12901 tgcattggaa gagatgaatc cttctttgat cagtgctagt agagatttag gcgcgaatag
    12961 ctttgagacc ttccgacgag tgatttttcc gttatcatta aatggtgtga aaagtggtgt
    13021 ccaagcagta tttattcctt ctctttcttt attcatgctg actcgtctga ttggcggaaa
    13081 tcgggtgatc actttaggaa cggctatcga acagcatttc ttggttacac aaaactgggg
    13141 gatgggttct acaatcggtg tgattttaat cattgctatg ttcatcgtga tgctcctgac
    13201 aggcgagaag aaaaaaggag gtcgctcata aatgaaaaaa tttaaatggt cgtatcttta
    13261 tttagggatc gtctttttat tcttatatgc accgatcttc tatctgattt tttattcctt
    13321 caatgaagga ggaacgatga acagctttac aggatttact tgggaaaatt atgaagctgt
    13381 ttttgaagat acacgtttga tcattatcgt attgaatacg ttcatgctgg ctttcttatc
    13441 tgcattgatc gccacgatca tcgggacatt tggggctatg ggaatctatt acacgaaaaa
    13501 acgacgtgca cggacagcgt tgttgagcat gaataacatc ttattagtct ctcctgatgt
    13561 tattatcggt gctagtttct tgattttctt tacactacta ggattcggat taggttttca
    13621 gagtgtattg ctttcccata ttgctttcag cattccaatc gtcgtcttga tggtcttgcc
    13681 aaaattgcag gaaatgaacg attcaatggt cgatgcggct agagaccttg gtgctaataa
    13741 tttccaagta gtcaaaaata tcattttgcc ttatctttct ccaggaatca tagctggata
    13801 ttttatggca tttacctatt ctcttgatga ttttgcggtt acatttttcg tcacaggaaa
    13861 tggctttacg acgctctcag tagagatcta ttctcgtgca agacaaggaa tcagtttaga
    13921 aatcaatgcg ctaagtgcgt tagttttctt gttctccatg atattggtca ttggatacta
    13981 ctttattagt aaagaaaacc gtccaaaacg gctgaagaaa aacagaagag tacgcgaggg
    14041 ggtggcacgg ctgcgatgaa aaaattacaa tctctgtttt taggtattct catcattatt
    14101 gcggtgctgc tgcttggtgt tcgtcaactt gaaaaaacaa gtggcatgag tggagcaaag
    14161 gtacttacca tctataactg gggagattat atcgatccta gtttgatcac gaaatttgaa
    14221 aaagaatatg gatacaaagt gaattatgaa acatttgatt ctaatgaagc gatgttcaca
    14281 aaaatccagc aaggtgggac aaactacgac atcgctatac caagtgaata tatgatccaa
    14341 aaaatgatca aagaaaaact agtacttcca ttagatcatt cgaagatcaa aggattaaat
    14401 aatattgatc cacgattttt agacttggat tttgatccag ataatcagta ttcgattcca
    14461 tatttttggg gaactttagg gatcgtatat aacgataaat tctttgatgc aaatcaaatt
    14521 aagcattggg atgatttgtg gaagccggaa ttaaaagaca gtttgatgct gattgacggt
    14581 gcacgagaag ttatggggct ttctttgaat agtttaggct attctttgaa tagtaagaat
    14641 atggcacagc tgacagaagc agcaaataaa ttgaatcatt taacaccaaa tgtcaaagca
    14701 atcgtggcag atgagatcaa aatgtatatg atcaatgaag aagcctccgt ggcagtgaca
    14761 ttttctggcg aagctgccga tatgatgtgg gaaaatgaac atttacatta tgtgatccca
    14821 ccagaaggat ccaatctttg gtttgataat atcgttatgc caaaaaccgt gaaaaataaa
    14881 gaaggtgcct atgatttcat caactttatg ctagaaccgg aaaatgcggc acaaaatgca
    14941 gagtatattg gttattctac accaaatgaa gaagcaaaag cgttattgcc aaaagacatt
    15001 tcgtcggacg aacaattcta tccatctgat gacacgatct cccatcttga agtctacaaa
    15061 gatctaggat cgaaatacct aggcatctat aatgacctat tcttagaatt caagatgtat
    15121 aggagataat ggcaaaactc agtaagaatg gggacttatc aacgttctaa agagctaaaa
    15181 atcgtccttt ttctatctga gttttccaat cttctaccaa aagcggatga aacgccgctt
    15241 tttttgtgtt attttatttt ttctaccacg tttttctacc agagaaaagt ggacaaaacc
    15301 attgtaaaat agggcccgag acgggctagt aaatctaaga ttgaaaaagt tacagaagga
    15361 taaagtaatt ggaaactatg caaatatact taacccttct gtaatctatt aataaaacta
    15421 atatatgaat ccagttcaat atttgtagct atgaaaaaac ataaaaatca tattagataa
    15481 aaactaaatt ggtagataaa ttattttgtc agatatctga ggcaaatatt cgactatttg
    15541 tgtttcaaag taaaaattgg aatacaaata tagaatagac atattgcagc cgatagttta
    15601 ttatgctttt agacacatta aactggagga aaaaatgaaa aagagcgcta aagatcgagt
    15661 ttttgatgag ctgatacgac taacagggaa tatgtcttct gtcactgccg agaaaatttc
    15721 gaagaatcta ggtatctctc gtcagaatgc cagccattat ttgacgagat tagtggaaga
    15781 taaaaaggtc gagaaattac cgggaaagcc tgttttgtgg aagcctttag atgaatatgc
    15841 ggttgtagat aatacggagc aaataaacga agcctttcat tctgtagttg gtcatgacgg
    15901 aagtctgaga gaggtcattc aaaaatgtat agccgctgtc aaatatccgc ctaacggact
    15961 cagtgtatta ataaatggag ccacgggtgt tggaaaaagt tttttagcca caaaaatttt
    16021 tgaatacgct atccatgagc aaattattga aaaagatgca ccatttgcta tcttaaactg
    16081 tgccgattat gcggacaatc ccgaactgct ttcggcaaca ctgtttggat acaagaaggg
    16141 ggcctttacg ggggctgaaa aggataccga tggtttattg gcaacggcca acaacggcta
    16201 tctttttctc gatgaggtcc atcgcctatc gaaagaaaat caagaaaagc tctttctatt
    16261 tattgatact ggaaactatc gtccagtcgg tgaaaatgta aattggcatt ctgctaaggt
    16321 tcgttttatt ttcgccacga cggaaaaagg tgaaaattat ttattagaca cctttgatcg
    16381 gcgtattcaa attagtgtta tgttgccaac ttttgaagat cgccccattc gagaacgatt
    16441 agagctgatt caattatttt ttcaaaatga agcggatgtt ttacaaaaag atattattgt
    16501 ttcaagagaa gccttgtctt taatgttatc tcatcccttt agtgggaata ttggtaagct
    16561 gaagaatatc attaaaatca gttgtgcgga tgtgtatagt cgaacacaag aagaaccatt
    16621 aacgattggg aaaaatgaga tcttgattca gttaaatatg ctggaatccg aagtagaaag
    16681 cttgccgatt gctagtttat tgataaagaa aaatcaaaag ataaaaacgg atcgaatcaa
    16741 accgtttgat tatgattcga ttttggaaaa aattactcag ctgataaatg aaggtaattt
    16801 caatgaaaat ttttcttcga taaagaatga agtacagcat ttggtgcatc acattgataa
    16861 ggattctttt ttatcaatga gtagaaaatt gatgattcag atgtttagaa aggtctgggt
    16921 atccgtttta ggaaaaacgt acggattgac gacgagtgga cctgttgcag aaattattag
    16981 cagatcctat gtcatttccc caagctataa gacagactta accgggataa atcgtacatt
    17041 agctgcaaat ctacctcgga cgacttactt atgtaatcag ttcttggagc atttaccacc
    17101 gctctcagaa aaggacgaga actttctaag actgcttatt gtcgtgactt tatctgatta
    17161 tgtagatgaa tccatcgagc tgaagggcct gttgcttgct cacggagata gtacggctag
    17221 cagcattcaa gcagtagtga atcaattatg cggcaattat gtttttgaag cgttggatat
    17281 gccagtagag agtaatgtgt cagagattgt cgaatcgaca aggaatttcg tgaaacggca
    17341 agtgaataca gatagtctag tgctgatcgt agatatgggc tctttaaatc aaatttacac
    17401 acagatcaag aacgatttaa aaggggagtt acttcttctg aataatttga cgacttccat
    17461 tgcacttgat attggcttga agatgacgat gaatgcgcca tttaaacaga ttgcggagga
    17521 tgcagcagac aagtattcaa tcaatgttca atactttgaa ggcttttctc aaagtagcaa
    17581 cattatcatt tcctgtatgt cgggtttggg gatttcggac aaattgaaag atgtttttcg
    17641 tcatcattta tcggatgaat ccatcgaagt tttcacgaag gactacagag aactacgtga
    17701 attaatcgac caaaatgatg aaacttattt tgacaagaca aaattggtca tcacgacttc
    17761 ggatcttcca cgttcctttt caattcctaa tattaatgtt tatgacatct tagacggtga
    17821 tggagcagcc aatttggagc atattttgag tcctgaaatg aagccggcag aatttaaccg
    17881 cttgattcag gagctagtaa aattcttttc gttggaagga attgcggacc gtttgaactt
    17941 tctgaaccca acgatcgtga tgagtgaagt ggaaacagtc atttctaaat atgaaaatta
    18001 ctatcatttc actttaaatg gccgtgtaaa actaaatctc tatatgcata cggcattgat
    18061 gatggaacgt ttattcttgg ctcggacaga aaaatctgaa gaaccgaatt atgatttgaa
    18121 accagatgaa gatgaatttt attatgtttc tagaagtatt tttcaatcaa tggagatgaa
    18181 atataatttt aaagtcaatg cttatgagct ttcgctgctc tatgaattat taggaccgta
    18241 tattcaaaaa taaagtgtct aaaaaagtgt ctaatctgtt ttttttaggt tagacacttt
    18301 ttttaatgtg aattaacgtg attaaaccaa tgtttttaaa agttggcaca ggaattgctt
    18361 atataaaagt gaacagaaag aaaggaatta accatatgaa gaaaattcta atagcgactc
    18421 acggtcactt agcttcaggc tttcttagca gtattcaatt actgacgggg aaggttcaag
    18481 agattacggt gattaatgct tatgtggatg agtgtgattt tgaacaggag ttgaatgtat
    18541 ttgtaaacca ggtgaacgag caggatcaga tttttgtttt taccgattta tttggcggaa
    18601 gtgtcaatca gaagattacc aaaacctttt tggagaaaga gattgctgta actattttgt
    18661 ctggctttaa tctgccaatt ctgctcgaaa tagtattggc aaccaaggaa ttaacaaagg
    18721 aggatattca ggaactggta aataaatgcc aacaggaatt aaagatgacc attcttgatg
    18781 aggtacatgt agtaaatgaa gaggaagatt ttttttagga ggaagaaaaa tgattaaagc
    18841 agtgcgaatt gatgaacgat taattcatgg acaagtagcg atgacttgga caaagagtct
    18901 aaatttaacc gggttagtag tagcaagcga tgaggcggct accaacgata ttcaaaagat
    18961 gacattgaag atggctgcac catcgaatgt caaagtcatt attaaaacag ttgatgacgc
    19021 gattcatttg ttaaaagatc cgcgggcgaa ggacatgcgc ttgatggttt tagtccctaa
    19081 tgtcaaagat gcggtaaaag ttgcgaaagc tttcccaacg gaaatcgaat taatgaacgt
    19141 ggggaatgct gggaaaatga caccgccatc ggaagataag aaaaccttat caaaggaagt
    19201 gatgttaacg acgtcagaaa ttcaagactt aaaagaatta gttgaacttt atccagatac
    19261 cgtttttcag cctacaccgg cgatggaaaa acgttcagct atctctattt tgagtgattt
    19321 ttagaaaaag gaaggagaaa aattatgtat gatgcatttg tagtcgcttt ggccgttttt
    19381 attggagttg ctggacatga aatgttcggg atgtccatgc tgagtcgtcc gattgttgtt
    19441 gctccgttag caggagcgtt gatgggggat ttgcagacgg ggttgattgt cggggcgtca
    19501 ctagaagcca tctttatggg tgtcgtcaat atcggaatct ctagtacggc ggagccttct
    19561 ttggctgctg ggctggctac agcgttcgca atcaaaatgg gaggaaacgt ggatgcgatt
    19621 attcccattg tctttccact agcagtatta ggattacagt taatgaatat gattttttct
    19681 tttgtatgtg gaccgatggc tgctaaattt ttcaaatttg caaaatctgg tgatcaaaaa
    19741 ggaatcatca atctgcactt ctttatgtgg tttgtccact atgcgcttta tgctcttatt
    19801 ccattctttg ccgttttatt tggagcagat gttgtcaaaa gcgtcttgga taatattcca
    19861 gaggtcatca tgaacggatt aacagtcgct ggaaatctgc tgccggcagt tgggatggcg
    19921 atgctgttac ggatgctttg gagtaaaaat attgctgtgt ggtttttctt tggggccgtt
    19981 gttatggcgt atctcgagtt accattgatt gcaattgccg cagttggaac gattattgca
    20041 attgttatag cccaaagaga tctacaacta aaaaatatat ctgttcatgc agttcaaagc
    20101 gatgctgctg taaatgcaac gcttgaccaa gaagaggagg atttctttca atgaaattag
    20161 ctgatggaat ttctaaagat gagaaaaaat tggttcggaa gatgttttgg cgctcgatga
    20221 ctatgtatgc ttcggtgaat ccggtgacga tgggcggcgg tggtttcgcc tattctatga
    20281 tgcccttcat taatcatttc tacaaaagtg aagaagatcg agctgcggcg ctggaacgtc
    20341 atacacagta tttttcgacg accattcctt gcgcgtcctt tgtcatgggg attgcggcat
    20401 cgatggagaa ggaaaattct gaaaaagctg gttttgacgt ggaaagtatc aacgcgatca
    20461 aaacgagttt gatgggacca ttggctggaa ttggcgattc attgttctgg ggagtttggc
    20521 gtgtcgttac cgcgggacta gcgatcaact tagccaatgc cggaaatatt ttggcaccaa
    20581 ttatcttttt agttatgttc aatataccta attacttgat gcgatactat ggcggcgttt
    20641 taggctattc attaggctct aaatatattg aaaagctgta tgcgaacggg ttaattgaaa
    20701 ttctgacaaa agctgctagt atcttgggtc tgatcatggt aggtgcaatg acgatcagta
    20761 tggtcaaatt caataccgtt ttgagctttt caatgaaggg cgaagaacta atgaatgtcc
    20821 aagaaatcct cgatagtatt tttaaaggaa ttgtaccgtt attggtaaca ttgagctgtt
    20881 tcaaattact agataaaaaa gtcggcgtca tcaaaattat ttttggaatg gtcgtcttgg
    20941 gaattttatt gtcattactt ggaatcgcat agtaggaagc tgcagctact aggctgcagt
    21001 ttttttagca aggagggaaa agatgactat taatgaagta atttattcac ccaaacaccc
    21061 attttttaat gctgagttaa acgacggaaa tgtgactact tcaaacgaat acagctacta
    21121 tcggaatatt cctgatgtaa agggattgac cttcgatgta agtaatcaaa agacattagc
    21181 aaaccttgat gtttgcggga cgattcaaac gttgaccttt tatcgagaaa atttactaac
    21241 tgaggaaaaa ccaggtgttt gggtcaataa acagctctct caaaccaatt cgttatcctt
    21301 aaaggttgaa gtaaacggga ttgtctacca tttgtcagaa tcggatcatc gtattgaagt
    21361 cgatgtgatt caagactgtt tgcctagata tattcatcat tattcgacgt ttaaggtcat
    21421 tgtagtatcc tttgctccca tactggctaa taagcgttta agtatgcttg ttcaacaggt
    21481 ttacattgtg aatgaaacac acgaaagcct tcaagtttca gtgaaagatg tgccttcgta
    21541 tcaagagaag tattcggatc agcaaaatgt gttgatttct caaaaaggaa ataaggacac
    21601 gatcgcacaa caggaagtgg caagcttcgc cattgctttt gtggacccaa atgcttttga
    21661 agaagtaatg acttttcagg aatctgatat cgaaaaatgg ctgaaggaaa cattgaacta
    21721 ttttgctcag ctatttgggc agctgtcaat gccaaacgat agagttgtcc atctctatcg
    21781 tcgtgcactt tatcaaagct tttctagttt cggtatgaat cgacagggac aggtcgtcgg
    21841 cagcaattgg ggcagctatc ctgctactaa tcgaatttgg aataaggata tgtattacgc
    21901 atcattgcca ttcaccatgt ttgaaccgga gttatgtcaa aaaacggttc tatggtttga
    21961 tcaatatggc gttaagtttc ctggttcaaa atttacagga ggtatcaatc attctctgag
    22021 taattcgtta tccagtgcgt tactggctag tctttatttt gaacatactg gggatacaaa
    22081 attctttgag cggtgtccgc aggttctact gaatgcttca agaattattg aggacatatt
    22141 agctcaacgt cggtcagggg aaccaatgct ttttccatct gtttggttat cagacgcctt
    22201 tgcacttgga aagtatcata ccggcagcaa tctttgtctt tggaaggcct gtattggact
    22261 tgcagaaatt tttgaagccc tagaacaact gagtcttagc aagcgataca aaaacattgc
    22321 ctgtaaacta aaggaatcaa tttgtaaaca aatgactatc gatggtcctt ttggtgagca
    22381 atttttggag ggaatcggtg atgaggagaa aacaacctat tcagtgaagc actatcaaaa
    22441 accgattctt gagcaagggt taatcttttt aagtgatgtg attgtggatg gaaaaattaa
    22501 tttattgatg catgatggag aagaatcaga cactacgttg atgccttttt atcaattttt
    22561 agataataaa gatcatttat atcagaacac catggccttt tctgctagtt ctttcaatcc
    22621 aacctatagc gaggaaatca aaggcatcac ttggggatta gaatccggcg ccacttttcc
    22681 tggctttatc actgttttaa tgagcgagct gcacaaccct caagctttcg taacccgatt
    22741 agaagaacta atttgtttgg cagatttaga cggttcgtgg tggtggtggc cgtatcgctt
    22801 agaggctcaa caaggagaag tcgtgcgaga ctttggctgc ggcaaatgcg gctgggcatc
    22861 gggtcttttt gtcagcctaa tgatcagtca atatttcgga ttgaaattca aaaaaggagt
    22921 attgcaaatt gatcctgttg ataatttaac gttttcttgg aagaatttga agttcggtca
    22981 agccttcatt tcgattgaat gtacgcaatc cgagttgagt atttcaaatt tgtcagaaaa
    23041 gccattgaaa ataagtgata cgaaaaaaat tcttcacgta gaaaagggaa aaacgattca
    23101 cattcaaagg agaaaaagat gagtatacta aaaaatatta cccaatttaa aaaaatccag
    23161 agtcgagcaa tcactcccga aaatccaaca ggcgagaaag gtaaagctgc gatgttgcca
    23221 agcgagctag gaccaagccg caaaggtcgt ggcgcaattc cgctgacttc tggtgaagaa
    23281 actgtgattg cggatattac tggaacggga ataattcgtc atatgtggat gacgattcgt
    23341 gatcgaacag aatttggcag ttttgtttta cgtgatttaa tcttaagaat ttattgggat
    23401 gggtcggata gccctgctgt tgaaagtcct ttaggggatt tcttttgtaa tgggtttgga
    23461 gagcgttgcg aggtgaactc gttgccgata gtggttaatc ctacaggggg ctttaattcc
    23521 tatttcgaaa tgcctttcca aacatccgct aaaattacga tcgagaattt acatccgaag
    23581 gatatgtcct cattttttta tacgataaat tacgctttgg tggacgaatt ggaagaaaat
    23641 agcctatatt ttcacgcaaa ttggcatcgg gaacgcatta ccaaattaca ggaggattat
    23701 aagttaattg atcgattaaa gggtcaaggc tattacgtgg gtacttacct tgcgcttacc
    23761 tgcctagaac gttattggtg gggcgaaggc gagtttaaat tctacattga tggggatagt
    23821 gagtatccga ccgtaacctc aacaggttca gaagattatt ttggcggggc gtgggccttt
    23881 catgagtacg atgagttcgg acgtccacat gcgaaaaact attcaacagc ctttttaggg
    23941 tatccttacc aatcaaatag agatgccaca agagatcgtt ttgaaacggg aaaattgaat
    24001 gccgttcatg cctttggtga cgacgcaatg ccacgacatg gtctttatcg ttggcatctt
    24061 caagacccca tctgttttac cgaagattta tcggtcactt tacaacaaat cggcaacgat
    24121 gatgttcgct tatttgagcg gcaagacgat gttgctagtg tcgcttactg gtattcaaca
    24181 gaagcttcag gaaatactaa acgtcatttc actgatcgag aacgcttgcc gcgttaatga
    24241 agtgtttttc acatattcga agggaggaac ctatgatgat atatggggca attgaagctg
    24301 gagggactaa atttgtctgt gcagtcggta ctgaggaatt aacgattttg gaaagagtca
    24361 gttttcccac aacaactcca gttgaaacaa tgaagcaggt cattgatttt tttaaacctt
    24421 ttaaggaaca attggcaggc atcggggtcg gctgctttgg tccaattgat attcaatgca
    24481 aatcaacgag tttcggtcat atcacatcaa caccaaaact tgcttggcaa aatttcaatt
    24541 ttgtaggaac gctaaagcaa tatttcaata ttccgattga atggacaaca gatgtcaatg
    24601 cggcttgcta tggtgaatat gttgctggtt ctggcaaagg gttagcaggt gttgcatact
    24661 tcactgtggg aacaggtatc ggaggtggtg ccttgttcaa tgggactttc gtggaagggt
    24721 tcagtcatcc ggaaatgggg catatgttga tcaagcgaca tccaaaggac aatttttcag
    24781 gaaactgtcc cttccaccaa gattgtttag aggggatggc agcaggacca gcgattgaaa
    24841 aacggttggg tgtaaaaggc cagaatttat tggctgacga ttcgttttgg cagattgaag
    24901 ccttttacct tgcccaatgt gcatacaata caacactgat gttttcgcca gatagaatta
    24961 tttttggcgg tggtgtaatg aaacaagaac acatgaagaa aaaagttcag gataaatttg
    25021 ttgagcttat aaatggatat gttgaaatac caccaattga tagctacatt attacgcccg
    25081 aattaggtga taatgccgga ataatcggcg gattagcatt ggccagaaaa gcggtaagaa
    25141 ataagcaacc ataattaatg aatgtttcat tgatactcat gtcaaaagta tttaaccgag
    25201 taatcaaatg atcaaatgaa gttggataaa gcgaaaatga attcaaataa aaaggctcta
    25261 agtcaaatcg tgttgttttc attaaatcac tgaaaggaaa tgcttctgcg tttccttttt
    25321 tctttttgtt gtgcgctgta taccttgagg atattcactg gtggcgtaca cttaaatagt
    25381 aatatattgt gttactttat gtctgtggta atagttatag ttgcttacca atgtatgttc
    25441 atttttcgaa atgttgactt cataatcctt ggcttaatgt ttgaattttt ttggtgatag
    25501 attttttcag ccgtata
//
LOCUS       DANKKG010000026        25500 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000026 DANKKG010000000
VERSION     DANKKG010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.026"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            131..3658
                     /locus_tag="PW004_000920"
     CDS             131..3658
                     /locus_tag="PW004_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HDL2725908.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3655..7377
                     /gene="addA"
                     /locus_tag="PW004_000921"
     CDS             3655..7377
                     /gene="addA"
                     /locus_tag="PW004_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HDL2725909.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7446..7787
                     /locus_tag="PW004_000922"
     CDS             7446..7787
                     /locus_tag="PW004_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2725910.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8096..9139
                     /gene="pheS"
                     /locus_tag="PW004_000923"
     CDS             8096..9139
                     /gene="pheS"
                     /locus_tag="PW004_000923"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HDL2725911.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9144..11564
                     /locus_tag="PW004_000924"
     CDS             9144..11564
                     /locus_tag="PW004_000924"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HDL2725912.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11734..12744)
                     /gene="trpS"
                     /locus_tag="PW004_000925"
     CDS             complement(11734..12744)
                     /gene="trpS"
                     /locus_tag="PW004_000925"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HDL2725913.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12998..13735
                     /locus_tag="PW004_000926"
     CDS             12998..13735
                     /locus_tag="PW004_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL2725914.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13749..14564
                     /locus_tag="PW004_000927"
     CDS             13749..14564
                     /locus_tag="PW004_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDL2725915.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14577..15224
                     /locus_tag="PW004_000928"
     CDS             14577..15224
                     /locus_tag="PW004_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDL2725916.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15240..15896
                     /locus_tag="PW004_000929"
     CDS             15240..15896
                     /locus_tag="PW004_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDL2725917.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16058..16879
                     /gene="racE"
                     /locus_tag="PW004_000930"
     CDS             16058..16879
                     /gene="racE"
                     /locus_tag="PW004_000930"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HDL2725918.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16882..18237
                     /gene="rph"
                     /locus_tag="PW004_000931"
     CDS             16882..18237
                     /gene="rph"
                     /locus_tag="PW004_000931"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HDL2725919.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18238..18756
                     /locus_tag="PW004_000932"
     CDS             18238..18756
                     /locus_tag="PW004_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL2725920.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18863..19363
                     /locus_tag="PW004_000933"
     CDS             18863..19363
                     /locus_tag="PW004_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDL2725921.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19643..20563)
                     /locus_tag="PW004_000934"
     CDS             complement(19643..20563)
                     /locus_tag="PW004_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HDL2725922.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20728..21741
                     /locus_tag="PW004_000935"
     CDS             20728..21741
                     /locus_tag="PW004_000935"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HDL2725923.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(21851..22606)
                     /gene="fabI"
                     /locus_tag="PW004_000936"
     CDS             complement(21851..22606)
                     /gene="fabI"
                     /locus_tag="PW004_000936"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HDL2725924.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22826..23266
                     /gene="fabZ"
                     /locus_tag="PW004_000937"
     CDS             22826..23266
                     /gene="fabZ"
                     /locus_tag="PW004_000937"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDL2725925.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23381..24142)
                     /locus_tag="PW004_000938"
     CDS             complement(23381..24142)
                     /locus_tag="PW004_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL2725926.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            24331..25425
                     /locus_tag="PW004_000939"
     CDS             24331..25425
                     /locus_tag="PW004_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDL2725927.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
BASE COUNT         8448 a         4424 c         5384 g         7244 t
ORIGIN      
        1 tttgcgtata cttaaaaaag tagaaatatc agaaccttaa aaggatataa tttgaagaat
       61 atagaacaaa tgttcagtat tttttgatat aataaaggag caccgtatca tgtgttgaag
      121 gaggaaaagc atgtctttac aatttattct tggaaacggg aaaaaagatc accgtcaagt
      181 cttgatcgac gaagcatccg cttggctcga ccaggatgca agtaaccaag tatttttcct
      241 tgttcccaac tacagcaaat tcgaacagga acaagaaatt ttagcggaga tgcgaagaag
      301 aagcggaaaa aaaactttca gcacaacaaa cgttcaagta tttagttttt atcgtctggc
      361 ttggtatttg ctgcaacaga caactcttct tacaggaaat gaattatcag aatcaggatc
      421 tgcaatgatc ttgcgacaga ttttagaaaa aaatgaggaa gaattaacaa tctttcgcgg
      481 agaaatcaat aaaagagggt ttattcgtca attacaagaa ctctacagtg aattgcgtac
      541 aggaaatatt caaccagaag acctgatgct tttatttggt gattcaccta atgcagaaga
      601 ccagcaactt aaaatgaaag acttgaagct gattttttca gcttatgaca gcgaattgac
      661 tcaacgtgca ctaacaaatg aagatgcact atctatacta gcaaattata tgcagacaca
      721 agatttcaac aacgtcaaat ttattgtcag tggtttttct cgaatcaatg gtcaagagta
      781 tcaattactg caggcaatga tggaaaaggg gcaattggtt gtcgatttat tgctggatcg
      841 tccttataca gcggatcttc cagaagtact tacgctcttt catgagactg gaaagcttta
      901 tttccgtctt tttcaaagtg caagagaacg gaaagtccct atattagtgg accgatttgc
      961 accagagcag actcgtccga cagagcttca actgctgcaa acaatgtggg aagagacatc
     1021 tagccaaaag aaagcggcag tacacaaaat agggggacaa gagcatctcc atttgtggga
     1081 agctgaaaat ccaactgaag aagtccgaca attagctgtt gaaatcagac gactagtcag
     1141 tgaagaaaac taccgttatc gtgatatcca agtgctgaca aaaaatatgt ctttatatgg
     1201 aaatatcctt cggccgatct ttcaagaaat ggcaattcct ttttatatcg atgaagaaca
     1261 aatgatggaa aatcatccgc tgatcgaatg gatcaacagt ctgttcgctc ttgaccgcta
     1321 ttcttatcgg ctaagtgata ttatgcgttt tttcaaaact gaattatttc tgtcgacgga
     1381 agcagaaacc gaacttgatg aatggaaaca agagcgagat gaattcagga aaaaactgga
     1441 catcactgaa aatgtagcac ttgcttacaa ttatcaagga acttattgga ctcgtgaaaa
     1501 agactggcag tttgtggcgt atgattttga agcagaacag attgaagacg tacagtattt
     1561 ggaagaacag tcaaatacga ttagacgatt atttcagaaa gctgtaccag tatttttgaa
     1621 aaaaataaag aaaagcaaaa caggacaaga ggccgcaaat gttttttatc aatttttgat
     1681 ccaaagtggc gtacagcaac aattattgtt ttggcgtaat caggaagtag acagaggaaa
     1741 cttagaagcg gcaagaaacc atgagcagac atggggagcg ctgattgatc tgttggatga
     1801 atatgtagta atttatggtg aatcggcttt tgactggtca ctgttccaag aaatcattac
     1861 aagtggtcta gaaaatctaa gttatggtaa aatccctacc gcaatcgacc aagtgcgaat
     1921 caatcgtttg gaattagttc gaccaaatca agctgccgtc acttttgcat tagggttgaa
     1981 tgatcaagtg tttccagacc gaaaagagac aaaaggattg ttgtccagtg aagaacgaga
     2041 agtattaaat caggccttgc cagatggaca gtttttattc gacccatcaa aggaaagtat
     2101 ctcttttgag cctttccaag cctatcttgt ttttctttcc ggaacagatc gattatatct
     2161 tagttatgca caaagctatg acacagatgg ttcgctgaag atgtctcctt atttgcgtag
     2221 aatcacagaa tatttatctg ttcccgtcga gagaaaagag catttgacga tcgaaagcga
     2281 ccctaattgt tatgtaggga cttacagaag cggaatcaat acagtcaata ggatttatcg
     2341 attagcttta gatgaaaaaa aacctgtacc aaagcactgg caaaagctca aagaattagt
     2401 actgaattcg tcatggcgaa attttgcttt acagattttt gaaagtcagg aacatttaaa
     2461 tgttcctgtt tcgatgccag aaaaaatggc ggaagagctt tatggaaaag agatttatac
     2521 ttcaatttca cgtatggaga atttttataa ttgtgaatac aaatacttca tgcaatttgg
     2581 tttgaaattg aaagaacgaa ctgtttatgg tctaactcca gcagcaactg gtgattttta
     2641 tcatgaatca ttggatcgtt tcttcaagct actgttttca aaccaattat ccttagtgaa
     2701 tatgtccgat gcgcagcgta gagactttac tgaaaaagtg ctgcaggaag tattcggaga
     2761 acttcggttt gagatattgg atagttctgc aagaatgaac tatatccgct accagttagg
     2821 acagacgatt caaaaagtga catgggcttt gcaaaaacag agcaaaaata caggcatgca
     2881 tcctcttcag acagagattc tatttggtca gattgctgga gcaaaaggta tcacaggtct
     2941 ggaattaccg ttgagtaatg gtggaaaact ccatgttcga gggaaaatcg atcgaatcga
     3001 tgtagcttct gaacaagaag atacttggct aagcgtcgtt gattacaaat ctagcggacg
     3061 ttcattcgat gtcacagaag cttattatgg catggctatg caattattga cgtatctgga
     3121 cgtagcattg atggatgcgg tacgtttgac tggaaaggcg gctgttaaac cagcaggatc
     3181 atattattta catgttcata atcctgtatt gacggagaca gatgatgtag agaagaatac
     3241 attgaaaaaa tttagttatg atggcgtttt tgtcgatgat cccgctcttt tagaagtact
     3301 agatcattca ctggaagcaa aagaacaatc actgatctat ccagtgaaaa aaaatgcgaa
     3361 agatgaatac caaaaaggta gtttcagtaa agacaaattt ttcacggaag aagaactgca
     3421 actattcatg gcgcataatc gggaaaatat gcggttagct ggagatgcga ttacttccgg
     3481 agaaatcaaa ctaaatccag cttataaaga taaacagcgg attgcctgcg aattttgtcc
     3541 attcagaagc atctgtacgt ttgatgtgat gctgaaagaa aataattacc accgtctgga
     3601 gaaaatagat aaggaagaag cactgcgtag aatattgaaa aggagtgaag aagagtgaat
     3661 caaattcctt taaaaccgca aaatgaacga ttcacagatg atcagtggca agcgattttt
     3721 gatcaaggag ataatctatt agtctctgcg tctgccggat caggtaaaac tacagtgctt
     3781 gttcgaagag tgattgaaaa actaaaaatg ggttttgata tcgatgaatt attgattgtg
     3841 acatttactg aagctgccgc aagagaaatg aaagaaagaa ttcaagaggc attacaagaa
     3901 tccgtcaata gcgaaagtga ccctgtacgt agacagcatt ttactaagca gttagtttta
     3961 ttgcccacag cgaatatctc aaccttacat gctttttgcc tgacagttat tcgacgttat
     4021 tattacttaa ttgatattga tccagtcttt cgcatgttaa cagatgaaac agaaacgatc
     4081 ttgatgaagg aggatgtgtg ggacgagctg agagaagcac tttatgcaga aaatgatgaa
     4141 cgatttttcc aattaacaat gaatttttct aatgatcgtt cagatgacgg cttgacgaat
     4201 ctggtgtttt ctctttatga gtttgcccgt gccaaccctg acccccaaaa atggctagaa
     4261 cagttaagcg ataattatcg tttgcctgag gggctagcga aatctcgatt atatcaaaaa
     4321 caaatccgtc ctttagtttt ggcagatata taccaatgtg tacagttata tgaacagatg
     4381 actcagttag cacaaggtga aggattagaa aaaatgcatg agcaagtagc tggagaacaa
     4441 cagcagatca agaatatcta tgaagcgttc tcgcaagatc ggttagaaga agcttatgct
     4501 ggtttggaac aactaacttt ttcaactttt aagagcagtc ggaaagcaga attgaaagaa
     4561 atttccaatg aagtcaaggg catgagggat aaagcaaaaa aactgattca gcagataagt
     4621 aaaagttatt ttcctgtttc cccaagccaa atggaagaat taacggacaa agcactaccg
     4681 ttagtcgaag aaatgacaaa agtcacgcaa agcttcatgg acggtttcag tatgcgtaaa
     4741 cgggaaaaag gtgtgctgga tttcaatgat ctggaacatt tagcgttgca gattctcaca
     4801 gaaaaaacga aagatgtatg gctgccaagt gaagcttcaa aacattatcg aaaaaaattc
     4861 aaagaagtaa tggtagatga atatcaagac gtgaaccaat tacaagaagc cattttatac
     4921 tggctaagag aacccgatga tacaaaagga aatatgttca tggttgggga tgtcaaacaa
     4981 tccatctatt ctttccgttt agcagatcct agtttattta tcgggaaata cgagaatttc
     5041 tcaaagaaag aaggcggacg tcgaatcgtt ttggctgaga acttccgctc tagaaaagaa
     5101 gtcctttctt ttacgaatct tatttttgaa caactaatgg atccagcagt gggtcaaatc
     5161 aattatgacg aagcagccaa actgatccaa ggattttcag actttccaga gaatgaacag
     5221 tttgagccgg aaatcatgat ctatgaaaaa gaacaggaag aatcagagat tgagattccg
     5281 acggatgata tactggaaga caaaacagaa ggcgaacttt ttatgacagg actaaaaatt
     5341 cgtcaattaa ttgattcctc ctttatgatt tatgataaaa aatcaaaaaa aagtcgtccg
     5401 atagagtaca aagacattgt tctcttgaca cctacaaaga agaacaactt aacgatttta
     5461 gagattttca aaacattgga tattccatta gagatgaatg atgctcaaaa ctattttcaa
     5521 gcgacagaaa ttcgcacgat gatctcgctg ctacaattaa tcgataatcc ctatcaagat
     5581 attccgcttg cggctgtatt gcgctcaccg atcgtgggtc tgattgaacc agaattagcc
     5641 agcattcgat tagctaaccg tgcacatact tactatgatg ctgtgcttgc ttatcaagca
     5701 tcaaacgaag atgaattagc agctaagctg gaacattttg gcaagcaact agagcattgg
     5761 cgagaacttg ctcgccgttc ttcgatcact gacttgttat gggatatcta ttatgagacg
     5821 ggatatctag agtatgtggt cggtctgcca gcaggggcac agagacaagc caatctttat
     5881 gcattggttg atcgtgccaa agcttacgag caaagttctt tcagaggatt ataccagttc
     5941 gtgcgtttca ttgaaaaaat gcaagaaaaa gataaagact tagcagaacc agtcatttca
     6001 atagaagata atgctgttcg cgttatgacg atccatgcaa gtaaaggctt ggaattccca
     6061 gttgtatttt tgttagatat gacaaaggag ttcaacttac aagacttgcg caatcggtat
     6121 gcttttgaag aaaaacttgg agcaggaatt cgctatatgg atcctgaaac acgagtgtta
     6181 tatgatacgt taccgtttca agccatcaaa ttggcaaagc aaaacaaatt gttgtcggaa
     6241 gaaatgcgta aattgtatgt aggattgact cgggcagaac aaaagctttt cattgtcggt
     6301 tcctataaaa ataaagagca aatgatccaa acgtggtctg aagcagctga tcatgaggag
     6361 ttagtttttg atcctgcgct acgtttgaag ggcagaagca gcttgatgaa ttggattggt
     6421 tacggattga tccgtcatcc tgaaatgcag aaatacttag aggaagaaat ttcgacttct
     6481 cttcttcaac attcaaatgc acagttttct atttcatgga tgaatcagca atcgatcatt
     6541 gaacaaagac agttgcttgc tgaaaaagaa ctggttaact tagatcagca gatgaaagaa
     6601 gatgaaactt tattagcaga ttcgttacaa aaacgtctgg catatgaata tccttatcaa
     6661 gcttcttcac aaaccacaag ttatcaatct gtatctgaaa tcaaacgatt attcgaagat
     6721 ccagatgata cacaagaatc acgtttgact ctggaaagca gtcagaacaa agcagcatcc
     6781 cgtcaatttc gttatacgca agaacaacta gcagaaccaa aatttttgca aaaagatcgg
     6841 caagtgtcag cagctgctgt tggaactgct acgcatgctt tgttacaatt actgccatta
     6901 gagatgccaa ctacagagag tatccatcaa aaattacagg aattggtaag gaaacggtta
     6961 gtagacgaaa aagtcgcgaa aaaagtagat gtttcttcta ttatatggtt ctttcaaaca
     7021 gaacttggtc aacagctgat tgccaacaaa gaaaacgtaa aacgagaaca acctttttcc
     7081 atgctgcttc ctgctgacga agtatttcaa gactatccaa atcaagaaga tgaactatta
     7141 atccacggaa tcgtcgatgg atacctagaa gaaaaagatc atctcaatat ctatgatttt
     7201 aaaacagatt ttattttaca tccggatgat ccagcagaaa tagatgcaat cgtccaaaaa
     7261 tatcaaggac agctaagact ttaccaacaa gcaatgtcag aagctttgaa taagccagta
     7321 gaaaatgttt tcctaatttt gcttcgagtg aaacaaatta taaacattaa caaataatta
     7381 taattgagca aacttgactt atgttttgta aggattatgc tatactctgg gaagaggtga
     7441 gtatgatgga gctaacacag acagaattcg ttttatgccc taaatttgaa aaaacattct
     7501 ctattttagg aaaaaaatgg aacggattga ttatcgatgt actactcgaa aacggtccgc
     7561 aacgctttgt tgagcttgcg gcaaaaatcc cgatggtcag tgatcgagta ttagtcgaac
     7621 gcttgaagga attagaaaaa gaaggaatag tcactcgaaa ctttgcctgt agcgaaaaga
     7681 gccgttcaga atatctcttg actgaaaaag gagcagactt gcaaaaagcg atgcgtcaaa
     7741 tacaagtttg gagcgaaaaa tggatcacag aggaagaatg cggttgattt tgtaaaaaaa
     7801 atcttgtaaa ctatagtcca tacgaataag taaagaacga tgacgaaaaa gagtagtttg
     7861 gctaaaccag ttcagggaga tctgtcgcag actgaaagca gatcctgtgt ttgaaaaatg
     7921 aagttcactt tccgagttcg tctgataaaa tcagaccggc aattgtcgtt aacgaagtaa
     7981 gagtgcggtg taagtcattt atccgcaaat taggatggta acgcgaagac ctcgttcctt
     8041 aacaggaatg aggtcttttt gttttgtatt tggaatttaa aggaggaaca agcacatgtc
     8101 tctacaagca caattagaga gcttgaaaga agcaacgctc gtcaaaatca cagcggcaca
     8161 agatttaaat gaattaaatc aaatacgtgt tgaaacgtta ggaaaaaaag gaccgatcac
     8221 agaagtatta cgtgggatga aagatctaag tccagaagag cgaccaaaag tcggaagttt
     8281 cgcaaatgag atccgagatc ttttgacaga agagatcgag cgaaaaaaac aagtattaga
     8341 agaagaagcg ttaaatgcag ctttggcaaa agaaacaata gacgtgacac tacctggtag
     8401 aaaagtaaaa catggtacac gtcatatcgt gacacaaatc atggaagaaa ttgaagatat
     8461 ttttgtgggt atgggctatc agatcgtaga aggatatgaa gtagaatctg atcattacaa
     8521 tttcgaacgg atgaatctgc caaaagatca tccagcaagg gacatgcaag atactttcta
     8581 tatttcagac gagatattga ttcggacaca tacttcacca gtccaagcac ggacaatgga
     8641 aaaacatgat ttctctaagg gtgctctacg aatgatctcg ccagggaaag tcttccgcag
     8701 agatacagat gatgcgaccc acagtcatca gttccatcaa attgaagggc tagttgttga
     8761 taaaaacatc acaatgggcg accttaaagg aacattagaa gtcgtaatga aaaaaatgtt
     8821 tggggaagat cgtgaaattc gtttgcgtcc aagttatttc ccatttacgg aaccatcagt
     8881 agaagtagat gtcagctgtt tcaaatgtgg tggtgccggt tgtaacgtat gtaaatacac
     8941 tggatggatc gaaattttag gagctggcat ggtacacccg aatgtgttga agatgtcagg
     9001 aatcgatcca gaagaatatt caggctttgc tttcggcctt ggaccagacc gcgtagctat
     9061 gttgcggtat ggagtgaatg atatccgtaa tttctatcaa aatgacttac gtttcttaag
     9121 tcagttcaag ggggagtaag aaaatgttag tttcatataa atggctcaat cgttatgtag
     9181 atttatcgaa tgtaacacca aaagaattag cggataagat gtctgtgaca ggaatcgaag
     9241 tagagggagt aacggttcca gaagaaggct tgaaaaaaat cgttgtcggt gaagtcaagg
     9301 aatgcgtacc acatccagat tctgatcacc tttctatttg tcaagtcgat attggcgaag
     9361 acgaactgtc tcaaatcgtt tgtggagcgc ctaatgtgaa ggttggaatc aaagtgattg
     9421 ttgcacttcc taactcacgt atcgctggta atgtaaaaat caaaaaagga aaaatgcgtg
     9481 gccaagtatc aaatggcatg atttgctcat tacaagaaat tggctattca gatagtgtta
     9541 taccgaaaga atacgctgaa ggtatctatt atatgccaca agaagctgta aatggcgatc
     9601 cagtttttcc ttatttggat atggatgatt cgattattga attatctatc acaccgaatc
     9661 gtgcggatgc attgtcaatg cgtggcgtag cttatgaagt tggggcaatt tatcgtcaga
     9721 ctcctacatt cgatgacgaa aagctggtag aatcctcacg tcctgctagc gataaaattc
     9781 aagtggaagt tgaagataaa gaagcagttc ctgcttatca aatcagaatc attgaaggag
     9841 taagaatcca gccaagtcct caatggctgc aaaatctttt gatgaacgaa ggcattcgtc
     9901 caatcaacaa tgtagtagat gtgacgaact atattttgct tttattcgga caaccactac
     9961 atgcatttga ctacgacaaa ttgaaatcaa aagagatctt tgtccgccgt gcaaaagaaa
    10021 atgaaacatt agtaacacta gatggagaat ctcgggaatt gacaactgac aatctcgtca
    10081 ttactaacgg caaagaacca gttgctttag ctggtgtcat gggagggtta gactcagaga
    10141 tcacgaatga aacaacaaca gttgcactag aatctgcttt atttgatcca atttcgattc
    10201 gtcgtacagc aaaacaattt aatttgcgta gtgaatcctc tgcacgtttt gaaaaaggaa
    10261 tcaaccatgc gactgttgga gaagccagtg aggttgctgc agcaatgatc gtttctcttg
    10321 cgggtgggga agttctacaa ggcgcggtca aaggctcaga gatcaaagca gaagacgtat
    10381 cagtgaagat ttcacttgaa cgcatcaatc aatatttagg aacatctctt gtcgttaaag
    10441 aagtaaatga gatttttgaa gcacttggat ttagctatga agaaaaggag tatgtttatt
    10501 ccgtagtagt accaccaaga cgctgggata tccaaatcga agcagatatt atcgaagaag
    10561 ttgctcgtat ttatggatac gaccagttgc catctacact tccaagtgga gaaacggttg
    10621 ctggaagctt gtcaaaagaa caagaaacta ctcgtaaaat tcgtacaatc tttgaaggaa
    10681 gcggattgag cgaagcaatc agttatgcat tgacaacaga agaaaaatca agacagtttg
    10741 ttataaatga aagtaatatt acacgtttgg actggccaat gagcgaagaa cgttctgtat
    10801 tacgaatgaa tttgatcagc ggactattag atgatatcag ttataacact gcccgaaaaa
    10861 atacagagat tgctttatat gaaattggtc gggtgttctt ccaagaaaat gatccacgta
    10921 cacatctgcc gcaagaagta aaacatgcag caatcgctat ttctggtatt tgggaagaaa
    10981 aagattggca gacgaaagct caaaatgtag actttttcac catcaagggt cttttagaaa
    11041 acttatttga acaactaggc atcactgaag atgtccatta tcaaacagtg acagaaatga
    11101 aggaaatgca tcctggaaga actgctgcga tttaccttgg cgaaaaattt attggttttg
    11161 tcggtcaagt gcatccaacg attacaaaaa gttatgatat tccagaaaca tatgttgctg
    11221 agtttgattt atctgctata gtggaagctg ctcaaaaagg gatcgttttc gaagcagtga
    11281 caaaattccc agcagtcagc cgggacatcg ctttgcttgt aaaagaaact gtgacaaatc
    11341 aggaattaac agaagttatc cgttctgctg ccggaagatt tttaacagat atccaattat
    11401 ttgatgtcta tcaaggagaa aacattgaaa aaggacataa atccatggcg tatagcctga
    11461 cattcgtcaa cccagaagca acattgacag atgaagaaat caataaagca atggaaaaag
    11521 tcgaaaaagc gttggtagaa aacctagagg caagtattcg ctaagaagga aaatatagta
    11581 agtaacgatc aagtagcttt tgaagaccat acatttgaaa aaaagatgtg acacaacttc
    11641 gtcacatctc tttttttgat tttaaaaaag gaatagcaca gagacgagat aaaaaaacag
    11701 gcctttacag cctgcttttt tagagtaaat gaattaatgg aagtaattga ttcccattgc
    11761 tgattttact tcagaaagtg tttgtgcagc tactttagca gcttcttcac tacctttacg
    11821 taagatttcc ataacagctg aaggatcttt ttccaattct tctcggcgcg cacggatcgg
    11881 tgcaaattca gcttccaata catcaatgag gtatcgtttg atctttacat caccaagtcc
    11941 gccgcggcgg taatgagctt tcatttcttc aattgcttct ttatctgttc cgaatacatc
    12001 caagtaggtg aagaccatat tcccttctac ttgtccagga tcttgtacat ggatgtggtt
    12061 tggatcagta tacatactca tgactttttt ctgtaagaca tcggcaggat cggagatata
    12121 aataccgttt cctaacgatt tactcatttt gccatttcca tcgattccag gtaatctacc
    12181 cattcctttt ggtgggaata cgccttttgg ttccaccaag acttcaccat atgtatgatt
    12241 gaagctttgt acgatttcct gtgtctgctc tagcattggt ttttgatcct caccaacagg
    12301 tacaagattc gctttgaatg cagtgatatc tgctgcttga gaaacaggat agataaagaa
    12361 tcctgtaggc acgctttcac cgaatttttt ttgttcgatt tctgttttga cagtcggatt
    12421 gcgccgaacg cgtcctacgc tgactaagtt caaataatac atggttaatt cagctaattc
    12481 cggaatttgt gattggataa ataaagtaga ttttgcagga tctaatccca cggctaaata
    12541 gtctaaagca acttgcaaga catttgatga aaccttctca ggatttttag cattatctgt
    12601 cagtgcttgc atgtcagcaa tcatgacaaa cagttggttg tcttcatctg cctgcatagc
    12661 cactcgggtt tttagagaac cgacgtaatg ccctaaatgc agcttcccag ttggtcgatc
    12721 tcccgttaaa ataatatttt tcaaaaaaga cacctcttta cgatttgtaa gtctgagcac
    12781 ttaatatctt accgtttttc ctgaaaagaa acaacagttc ctaaggaaaa aacaaggaaa
    12841 gaggataaaa taactaatag attagaaatg gaagaaatat ttatgggaga ataaacgttc
    12901 taagaaactt tacaaattac taaactatca gatataatgt attttattat acatctttaa
    12961 aagaaatctt actaaataat aatgggagtg taattctatg gcaatgatta aatttgatca
    13021 cgtggataaa tattatggca agttccacgc attaaaaaat atcaatctcg aattcgagaa
    13081 aggagaagtt gtcgttgtca ttggtccttc tggttcagga aagagcacga tgcttcgctg
    13141 tatcaatgga ttagagacga tctcttcagg taagctgctg attaatgata ctgacttgca
    13201 tgataaaaag acaaaactga cagaagtccg aaaaaacatc ggcatggttt tccaacattt
    13261 caatttgtac ccaaataaaa ctgttctgga aaacatcaaa ttagcaccaa tcaaagtgtt
    13321 gaagcaagac caggcaactg cagtcaaaaa cgcggagaaa tttttgaaaa ctgtcaatat
    13381 gttagataag aaagattctt atccttccat gttatccggg ggacaacagc aacgggtggc
    13441 aatcgccaga ggtttagcga tgaatcctga gatgctgctg tttgatgaac ctacttctgc
    13501 tttggatccg gaaatgattg gtgatgtatt agacgttatg aaaaaattgg ctcatgacgg
    13561 tatgtcaatg attgtcgtga cacatgaaat gggctttgcc aaagaagtgg cggatcgtgt
    13621 tatcttcatg gctgatggag aagtattgga agacagcaga aatgtgagag actttttcga
    13681 aaatccaaat gaagtccgag cacagcaatt tatcagtaaa gttatcaatc attgatagga
    13741 ggaaaaagat gcgcaggtta aaaaaaatac gcctgatttt ccttttaggc attgcttttc
    13801 ttgcactagc cggatgtaaa ggaaatgggt tggcaaatga agatatttta gagcgaagca
    13861 aatctaccaa tgaaatcatc tggggtgtca agtatgatac tcgattattt ggaatgatgg
    13921 atattgagag cagaactgta caaggatttg atgtagatat tgcaaaagca atcactaaaa
    13981 agatccttgg tgacaatgga aaaacagaat ttgttgaagt cacttctaaa actcggattc
    14041 cattattgaa aaacggaaat atcgatgcaa ttattgcaac gatgacgatc acagatgaac
    14101 gaaaaaaaca ggttgacttt tcagatgtct atttcgatgc tggccaagct ttgttggtaa
    14161 agaaaggaag ccaaatcaaa agtgtagatg atttgaatgc ttctacaacg gttctagcag
    14221 tcaaaggttc tacatcggct gcgaatatcc gacagcatgc accagatgca aaaattttag
    14281 aattagaaaa ctatgcagaa gcatttactg ctttacaatc tggtcaggga gacgcgatga
    14341 ctacggataa tgcgattttg ttagggattg cagatgaaaa tccagagtat gagttagttg
    14401 gtggaacgtt tactaatgag ccttacggga ttgcgatcaa caaagggcaa gaaaacttct
    14461 tgaaagctgt taaccaagcg ctagaggaaa tgcatgcaga tggcacatac gataaaatct
    14521 atcaaaaatg gtttccaaat gaaactgaag gaaaagtaga gtaaggggga gaggcaatga
    14581 gcgaattgat acaaacttat ggaccaatct ttgtcgacgg gttcaagatc acattgtttt
    14641 cgagtgtctt agcacttgtg tttagtttga ttatcggaac gttaatggca atcttccaat
    14701 tatcaaagaa taaagtagtg agcgccttag caaaggcata tgtagaattt tttagaaaca
    14761 ttccattatt aatcatcgtg atgttttttt atgttgtcgt accgatttat tggtttaatc
    14821 tagatggatt ccaagcaggt acgattggat taacgatcta tacatctgct tttattgctg
    14881 aaacagtacg ttcagggatt caaaccgtac caaaaggtca aacagaagca ggactatctt
    14941 ctggttttac ctattcagaa atcatgcgtt atattgtttt accgcaagca ttcaaaatcg
    15001 ttatcccgcc attaggcaac caatttatca acctcgtaaa aaactcatct attttagcaa
    15061 tcgtagctgg tttagatttg atgtatcaag gagacttgat cgctagtgaa acattcaata
    15121 ccttcgatac gtatatcatc gttgggttat tttacttagt tatcacgcta ccgttatctt
    15181 acttaatggt ttatcttgag aaaaaatggg cggttcgttc atagaaggag gggaaataca
    15241 tggattttag cggcgctttt tcatggatga atatccgttt tttattggaa ggattaaagg
    15301 ttactgttga agtttcattg ttttcgatca tttttagttt cttgatcggt ggtctgacag
    15361 gtgtgattcg ttttgcgaat atccctttct tttccaaaat ccttggtgta gtgattgata
    15421 ttatacggaa tcttccatta ttattgatca ttttcttcac ttactttgcc ttacctcaaa
    15481 tcggcattca gatgaacatc ttctggtccg ctgtcgcagc attaacgatt tttgagtcag
    15541 ctatgttgtc ggaaattttc cgagctggac tcaatgctgt tccaaaagga cagatggaag
    15601 caggattatc aacgggactg acttatgtag aatcgatgcg aacgatcgtg ttaccgcaag
    15661 catttaaatc aatgatcccg gcaattgtga gccaattgat ttcactgatc aaagatactt
    15721 cattagcagt aatcatttct ttacctgaac tttctcatca ggcacgtatt atttatggac
    15781 agaacaccaa ttatgtgctt ccgatgttca tcatcatgac acttatgtac ttcgtggtat
    15841 gttacgcgct ttctctgctt tccggttact tggaaaagag acggtacagt tattaataga
    15901 tgattctttt agcggttgtg gcaaactaaa gtcacaaccg cttttcctct ttccattcga
    15961 aaaaaagaga tttcgattgt tttaagcaag taagagcact tgaaaattca cgctaaaaag
    16021 tacacaatag ggaggataga atttattgaa aaaaaggatg atacgattga cagataatcg
    16081 ccctatcgga tttattgatt caggtgtcgg cggcttgact gtagtaaaag aagccctgaa
    16141 acaattaccg aatgaaaata ttttatttgt aggagacaca gcacgctgcc catatggccc
    16201 tagacccgcg gaacaggtaa tccagtatac ttgggaaatg acggattatc tggtggagca
    16261 aggaatcaag atgctggtga tcgcctgcaa taccgcaact gcggtggctt tagaagaaat
    16321 caaagctgct ctttctattc cagtcatcgg tgtgatcctt cccggtacta gagcggcagt
    16381 aaaaaaaaca caaaataaac aagttggcat tatcggtacg attggtacgg taaaaagtca
    16441 agcttatgaa aaagcactga aagagaaagt accagaattg actgtgacaa gtcttgcttg
    16501 tcccaaattt gtttcagttg tcgaaagtaa tgaataccat tcatcggtgg cgaaaaaaat
    16561 tgtggcagaa acattagctc ctttaaccac taaaaaaatc gatacattga ttttgggatg
    16621 cacccattat ccattattac gccccatcat tcaaaatgta atgggagaaa atgttcaact
    16681 gatcgattct ggagcagaaa cagtaggtga agtatctatg ctgttagatt atttcaattt
    16741 gagcaattca ccgcaaaatg gtcggacatt atgccagttt tatacaactg gctctgccaa
    16801 acttttcgag gaaatagctg aagactggct tggaatcgga cacttaaatg tagaacatat
    16861 cgaattggga ggaaaataag gatgcgtcat gacgggagaa aagcccaaga gcttcgcaaa
    16921 ctggagcttc aaacaaatgt tttcaaatat ccagaaggat cggtagtgat ccgttttggt
    16981 gatacaacag ttatttgttc tgcaacaata gaaaattctg ttcccccttt tttgagagaa
    17041 acaggaacag gatgggtgac agcagaatat agtatgctgc ctcgagcaac gagtacgaga
    17101 aatcgtagag aaagcagcaa agggaaactt tctggtagaa caatggaaat ccagcgtctt
    17161 attggtcgtt ccttgcgtgc agtagtagac ttggaaaaat taggagaacg tagtatcatc
    17221 gtagactgcg atgtgatcca agcggatggc ggtactagaa cagcaagtat cactggggca
    17281 tttgtggcat tgaaattagc cattgaaaaa cttttaagag aaaaagaatt gtcagaagat
    17341 ccaataaaag aacatcttgc tgcagttagc gtaggaatct taccagatgg gacttgcgtc
    17401 acagatttgg attaccaaga agattccgca gcacttgtag acatgaatct tgtaatgaca
    17461 gaatctggaa aattcgtgga gattcaagga actggagaag aagcaacgtt tgatggtgaa
    17521 cagttgaatg aaatgctctt ttttggaaaa aacgcgattg aagatttgat caaagagcaa
    17581 aaacatgctt tgctaacaga gtttgctcaa aatgatgagc ggatagaaga aacaaaaaca
    17641 atcatcattg cgacaagaaa tccaggaaaa gcagaagagt ttcgaaatat gttcaaagaa
    17701 gcgggatatc atgtaaaaac attacttgac tatcctgaat tgccagatgt ggaagaaact
    17761 ggcagcacat ttgaagaaaa tgcccgatta aaagcagaaa cgattgcaca attgcttgat
    17821 cagccagtct tagcagatga ttcaggtttg aaagtggatg ctttaggcgg tatgccagga
    17881 atctattctg cacgttttgc aggggaacaa aaaagtgatg cggggaataa cgccaaatta
    17941 ttatatgaat tgacagatgt gccggatgaa aaaagaacag cacaattcca ttgtacgtta
    18001 gtattcgcgg cacccaaaaa agacagcctt gtagtagaag cggaatggcc cggtcgagta
    18061 gcccgtatac catctggcga aaatggcttt gggtatgatc ctttatttat cccagaaggt
    18121 aagaaacaaa cagcagcaga attatcgagc gaagaaaaaa acaagatcag tcatcgggct
    18181 caagcaatga aaaaattgag tgccgagtgg aaacaatggc tggaaggaga gagatagatg
    18241 cggtatttag tagtaagtga taatcatgga gacagagata tcctcgttga cttagtagat
    18301 cgttatcgtt ctgaagtgga tatctttttc cattgtggtg attcggaatt agaagcttct
    18361 gacccattat gggatacatt ccaagtggtt caaggtaatt gtgattatgg accaggattc
    18421 gaacagaaaa aagtgatcca aactggtcag gatacagtat tcatgactca cggtcattta
    18481 tcgaatgtcc gatttggttt gacacagtta gcaatcgaag ctcgagctgc aactgccgat
    18541 atggtgtttt ttggtcatac ccatcaaata ggctgtgaga tggatcagaa aatcctgttt
    18601 ttgaatcctg gtagcatttc tcagcctaga ggcccgatcc aaatcccatc atatgctctt
    18661 atcgaaagtt tagatgacca aatttctgtt caatactata accgtgccca tcaggcagtt
    18721 gaagagttgg ccttttcttt ttcgagagag cgctgaataa aaaaactcta atttttaata
    18781 aaaataagct ttattttatg aattttgcat ccggtttcgg taaaatagta gtgttgaatg
    18841 aaatagaaat ggaggccatc agatgattgg acctatagta agagaaatgc tgctggaaaa
    18901 tcaggaaacg tttttgattc ctgcagaaaa tgtggcaaat gttatgtatc atcatccatt
    18961 gtcccatggt ttacttgtgt tatcgaaggt cggttattcc aaaattcctg tattaggaaa
    19021 agatgatcac tttgtaggac tagtaagttt atcagatgtg gtcaataaaa tgattgactt
    19081 gcaaacaatc agcatggacc cgttagaagg actaacggtt gctgatgtga cagaaacaga
    19141 tgttccgaca attgatgaaa attgggaact agaagaagta ctccatttgt tagtcgattc
    19201 gcctttttta ccggttgtca cagaagatcg gattttcaaa ggaatcatta caagaaaaga
    19261 gctgcttaaa gccgtaaact tcatggtgca tgaattagaa cgtcgtaata tattgtctcc
    19321 gaaaatcgaa gaattaaaag aacaaatcga tatcgtgagc taaagaacga gaaactaaaa
    19381 gataatcaaa aaataagtag aaaaaacagc gtaccagttt tgactttatc ttgaaaaaga
    19441 ggctgggaca aaagcgttta actccaagaa ataagaagga attcacgaaa attgcttttc
    19501 aaatttttgt gaatttcagc ttatttccga aggagttgct tctgcttccg ccgtttatac
    19561 gtgtttagag cgtgggacaa aagtgttctt tacttttgtc ccacgctctt tttagtgaga
    19621 ttcgttgcat cgtttgtcca aattattttc cgacagcaat tggactgttc ggaatcgtga
    19681 aaccagaagc tgtcaattct ttgacatagg agctcaagaa ctcttctttt aaggcatatt
    19741 gttttccgtt caatgcgtag cagaccgtac gaatcgcaaa attgctgttt ccaagatcca
    19801 ctaatccaaa gatagaaggt tcagtttgga gctcttcttg gtatttttca gctaatgtac
    19861 gatttactcg atcaatgatc tcatagattt tgtcatatcc ttcttcagga acaatccgaa
    19921 tatctaaaag tacttgcata tttgcacgag agagattgct gatcgtggtg atgtttcgat
    19981 tcgggatgaa atggacagta ccatccgttg ctttcagctg aagggtacgg ataccaacag
    20041 atacgacggt tccttcaatc gttaagtttg ctagcttgat gtagtcccca acatccatct
    20101 gttgttccat gataataaaa aatccggtaa taacatcact catgaaccct tgggcaccaa
    20161 gaccgattgc cacacctgca attccggcac cagccaaaag tgatccgatc ggcacaccga
    20221 tagcagataa aattgcatag ataaaaaaga aaccaagggt ataatgaaaa atagtattta
    20281 aaagactatg taaagtcttt aagcggctct cgtttagaat ggctttttta gaatactgat
    20341 taaaagactt atcaatcaga tattttccca cacggtgaag aatccaaaaa aggacgatca
    20401 aaaaaatcag atataaactt ttttggataa aggcagccaa aatggcgtcc caatcaatcc
    20461 cgtcccaaaa ccgctgaaac gcatttactt tgctgacagc ttgatctgtc aatgtctcgc
    20521 cagaagagtc tgtcgtaccg gcagttgcta ataatgaaaa cataaaatcc tcttttctaa
    20581 aatttgattc tttctgtatt gtaccaagaa gttttcaaaa aaggaaatag caaaaataac
    20641 tataatgaag gaaaaaagct gaaaataaaa tgacaaataa tatggataat gataaaatgt
    20701 ggagaaaata acgaaggtgg taagtatatg caaatcgatt ggaacaatct aggtttttct
    20761 tatatcaaaa ctccttggcg ttttattgcc aaatggaaag acggacaatg ggaagaagga
    20821 gaattgacag aagataatta cctgacgatt catgaaggct ctccagcgct tcattatggt
    20881 cagcaatgtt ttgaaggtct aaaagcttat cgtcgaaaag acggcaaggt caatctcttt
    20941 cgacctgaac aaaatagtcg acggatgaat caaagtgcta gacgattgct gatgccaatg
    21001 gtccctgaaa agatgttcgt tgatgctgtc aagcaagtgg taaaagcaaa tgaagatttt
    21061 gtgccgccct atggaacagg cgctacatta tacttgcgcc ctttactgat tggggtcggt
    21121 gaaaacattg gtgtccatcc agctccagaa tatttatttg tcgtcttctg tacgccagta
    21181 ggagcgtatt tcaaaggtgg actaaaacca acgaatttta ttgtatctga ttacgatcgt
    21241 gctgctccta aggggactgg tgcagcaaaa gtcggcggaa attacgcagc gagtttactt
    21301 ccaggaacag aagcaaaaga actggattac tcagattgtg tttatcttga tccagccaca
    21361 catacgaaaa tcgaagaagt aggtgcagca aatttctttg gaattacaaa agacggaaaa
    21421 ttcatcactc cgcaatcgcc ctctatctta ccaagtatca cgaaatattc cttgttgcag
    21481 ttagctgaag aacgactagg actgcaagca attgaaggag atattttcat tgatgatcta
    21541 gatcaatttg cagaagctgg tgcatgtggt acagcggctg ttatttctcc aatcggtggg
    21601 atttatcaca ataacaatct tcatgtattt tatagtgaaa cggaagtagg accagtaaca
    21661 aaacggttgt atgatgaatt gacaggtatt caatttggag acatagaagc accagaaggc
    21721 tggattgaag tcgtggaata atttcaaagt aatgagttga aaaaaacgta aaaagaaaaa
    21781 atcaggaaga gactttaaaa agttttttcc tgatttttat atgttttact ttaaagaatg
    21841 caacaaaagt ttatgaaaga tggacacctt tgtcaacgta gataatatca ccgatgattc
    21901 cgctggaaag tgggctgatt aagaaagcac aagcattacc gacttcttcg atcgtcacac
    21961 cttgctgatc aggcgtacga gattcagata actggatcag tttttgatag tctttaacac
    22021 ctgtgacagc taatgttttg attgccccag ctgagattcc attgacccga attcctttag
    22081 gcgagaactc agctgccaaa taacggatag cagcttcaag agaagctttc gcaataccca
    22141 tcatgttata attcgggatt gcgcgttctg aacctagata agtcatgcta acgatcccgc
    22201 tgtttggtgc aagaaacggt tgtgcgtatt ttgatacagc aattaaagag tagctgctga
    22261 tatcttgagc taattgatag ccgtctctgg aaatatcaga cacatttccg gacaattctt
    22321 ctttgttggc gtaagcaact gcatggacaa ggccgtggat ctccccagca tattcaccga
    22381 tggcattcat tgctttttcg atgctttcgt cgctagaaac atcacattct acagtgaatg
    22441 cttcttcgcc gaccaatttg actaatgatt ttttcattcg ttcattttgg taagtataga
    22501 taatttctgc cccttgtttc aacattgctt cagcacaacc ccaagcaatg ctttttttgt
    22561 tagcgacgcc cataatgacg attttttttc cttctagaaa tgacataatg actctccttt
    22621 gtttctgata aaaatttgat agtcgtaaaa aaatagagaa taatagctaa aataacttct
    22681 tgatttctta attattagtc aaaatgtttt gattgtcaaa ctattttttc ttaaattaaa
    22741 aaaagattgc taaatattta ggaatatgat actttgatag tcaaagtaat gaaaatggca
    22801 attgcctgaa agaaggagtt taattatgtc ttacgtatta tctgctactg aagtaatgga
    22861 attgattcct aatcgttatc ctatttgtta tattgattat gtagatactt tagaaccagg
    22921 aaaaaagatc gttgcgacaa aaaatgtaac aatcaatgaa gattttttcc aaggtcactt
    22981 tcctgacaat ccagtgatgc cgggtgcgtt gattctagaa acactggcac aagcaggttc
    23041 gatacttatc ctaaaatcag aagagtttct aggaaaaaga gcttatatcg gtgggatcaa
    23101 taaggccaaa ttccgtcaaa aagttgttcc aggagatgtg ctgaaatgtc attttgaaat
    23161 caccaaaatg aagggatcag tcggtacagc gttatctgca gcatatgtag atgataaaaa
    23221 agtctgtgag tgcgagttca cgttcatcgt caaccaaaat gcttgactat aaaacctgat
    23281 atagcaaaac aaaaggaagc ttacttgctt tttatgtaaa aagaagacgc ctgaatccta
    23341 gcatacgttg tcatctatgc aggattcaga cgttattttt ttatttttgt tttgcttctt
    23401 gcagtatatt gattttatcg atttttactt cttcttttgg tttgtctgaa ctatctgttt
    23461 cgatatttgc aatagcatcc acgacatcca ttccttcaat cacttggccg aagacagtat
    23521 aatggccatc tagtgaagga taaccgccgt ttttataagc gtcgatgatt gcttgaggat
    23581 agtcgtcttt taaaagacca tcatgcatat catctgtatt ttgaacgata aagaactggc
    23641 tgccgttact gttaggatct tgtgagcgag ccatagataa cgcacctctt atattataaa
    23701 ggtggttgtt gatctctgtt tcaaatcctt ctccccaaat gctttcccct ccagtgccat
    23761 cgcctttagg atcgccacct tggatcatga aatctttgat gacgcgatgg aaagtcagtc
    23821 cgttgtagta accgtctttt gcatgtgtca taaagttttc aaccgctttt ggtgcgtgtt
    23881 cgggaaataa tttgattttg attgtgcctt tattcgtcac catttcgacc agatcttcat
    23941 tttcggcgac atctttcgtc aattgtggta attccaatgc gttcaaatcg acgctactgg
    24001 cagtcgtgct ttccgcagta gatgtacttt ttgaactttc tgtagacgaa ctagaagaag
    24061 tcgtgttgtt tgcagaacaa ccagcagcaa acagcaatgc tgctgctaaa ctaatactta
    24121 aaataacttt atttttcttc ataactgttc ctttccaatt tactattttg ttatctttaa
    24181 tgataacaaa atagagggaa aatacctact taaattattg gaatttatca tttattttaa
    24241 aagaaaaaga aaaggtgtag accatttact ttgtgtttgc tataatgtaa gtggatacaa
    24301 aaacgaaaag atgcaggagg aaatggtaat atgggaaaat taggaatctc gatttatcca
    24361 gaacggtcaa catttgaaaa ggacaaagcg tacttggatt tagctcataa gtatgggtat
    24421 aaacgggttt ttaccagttt gctgcagatc aatgatgata aagaaaaagt tctttcagaa
    24481 tttaaagaag tagttgatta cgcgaatagc ctaggcatgg aagtaatggt tgatatcaat
    24541 ccagcgttat ttgaacagtt ggagatttct tacgatgact tatctttttt ccataagatg
    24601 ggtgcctacg gggtccgctt agatatcgga ttcacaggag cagaagaagc aaaaatgacg
    24661 cgcaatcctt ttggtatcaa aatcgagatc aatatgagtt cgggcacgaa ttacgttgac
    24721 aacatcatga gttattcgcc aaacacggat aatttattag gttctcataa tttttatcct
    24781 catcgatact ctggtttagg atacgaacac tttgtttttt gttcagaaaa attcagaaaa
    24841 tataatttga atacgatggc attcgtcaat tcgcaatctg ctgagtttgg cccttggcct
    24901 acgcaagatg gcttgtgcac gctggaagac caccgagatc ttgaaattgc gacacaagtg
    24961 aagcatttaa tccttacagg attgattgat gatatttcta ttggaaatgc gtacgcaagt
    25021 gaagaagaat tgaaagaaat ggcagaagca tttaacgcgg attatccaac attgaaagta
    25081 gatacggaag aaggtattac agaaaacgaa cgaatctgct tgtttgataa ccttcatagt
    25141 tatcgagggg atcgctcaga atatatcctt cgttcaacga tgactcgtgt atactacaaa
    25201 gataaagatt tcccgcctca taatacgcga gatatgcatc atggggatgt actgattgac
    25261 aatgaagggt acggtcaata caaaggagaa acacaaatcg ccttgaagga tatgaaaaac
    25321 gacggacgtg tcaatgttgt aggaagaatc tccgatgacg aattattcct ccttgacttt
    25381 ttgaaaccat ggtcaagctt taaattaatt gaaaataaaa aatagataat tagttattgc
    25441 agaaataatc cgctagaaat agccagttat ttctgctttt ttattttgtt tttttatagg
//
LOCUS       DANKKG010000027        25228 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000027 DANKKG010000000
VERSION     DANKKG010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.027"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..519
                     /locus_tag="PW004_000940"
     CDS             <1..519
                     /locus_tag="PW004_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725928.1"
                     /translation="VQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESSTTVSDANILANIILDFIFEHDIPFKNGYEILPQNEQYFFYKCLTNRVCCSCGKK
                     NADIHHVDSVGMGNNRKKINNSGRRFMALCRECHTKIHVEGFTTFTTKRKLTAVVLKD
                     SDLKRLGLNIIE"
     gene            527..1042
                     /locus_tag="PW004_000941"
     CDS             527..1042
                     /locus_tag="PW004_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDL2725929.1"
                     /translation="MDIEGYEGVYQVSSLGRVKSNRPNYVINKGYVNKEGFIMKPSDN
                     GKGYQIIFLSGKGFRDRRYVHRLVAFHFLKEAYFEGAEVNHKNGDKSNNTVENLEWLS
                     SDDNKKHARKNGLTNLKGPSKLTDLQALAIKRLYTNKLMSSGEIMKLFNVSRHTVLNI
                     ASGKTFSFLED"
     gene            1147..1977
                     /locus_tag="PW004_000942"
     CDS             1147..1977
                     /locus_tag="PW004_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HDL2725930.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            1994..2845
                     /locus_tag="PW004_000943"
     CDS             1994..2845
                     /locus_tag="PW004_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL2725931.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALEKAKSYTNEVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNTELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            2842..3201
                     /locus_tag="PW004_000944"
     CDS             2842..3201
                     /locus_tag="PW004_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HDL2725932.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQA
                     PLILAKKYRNTFKEERF"
     gene            3216..3377
                     /locus_tag="PW004_000945"
     CDS             3216..3377
                     /locus_tag="PW004_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDL2725933.1"
                     /translation="MTTEEVIQMRIRNIQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            3374..3679
                     /locus_tag="PW004_000946"
     CDS             3374..3679
                     /locus_tag="PW004_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725934.1"
                     /translation="MSVDKKATMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            3679..3996
                     /locus_tag="PW004_000947"
     CDS             3679..3996
                     /locus_tag="PW004_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HDL2725935.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVKKYKEILDKYME
                     DSK"
     gene            3993..4388
                     /locus_tag="PW004_000948"
     CDS             3993..4388
                     /locus_tag="PW004_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725936.1"
                     /translation="MILKYRAWNKKTQSFIDYGDLVLDLRSGKIYAGDIGLVESTIDV
                     TDQIELMQSTGMKDKNGVEIFEGDVVITSKGAIGYVAYLQQEAGFVVVLKKSDYRLGH
                     RNTGESYDVATAHEVIGNIYENSELLEVE"
     gene            4385..4723
                     /locus_tag="PW004_000949"
     CDS             4385..4723
                     /locus_tag="PW004_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725937.1"
                     /translation="MIILATDYIDSLKDENGNVPALLNCGIKSKNRIILLRISADLAK
                     YLYLSKTAVYYTAATRYQYKGKTISQDYYDRLISPDMHGKSKSAIKLFGAIEIYADDF
                     PNLWLKEEAE"
     gene            4720..5088
                     /locus_tag="PW004_000950"
     CDS             4720..5088
                     /locus_tag="PW004_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725938.1"
                     /translation="MNEEIKKSIRYIDRALSQLEPLRIESHKGMSVYPNQYDKMNADL
                     RNAKLALINIKNNKGCQYCKHSEPLNDTSNSNFATAIECEENGYYIATEYDDGDYADN
                     GYSAYIQYCPMCGRKLEVAE"
     gene            5085..5390
                     /locus_tag="PW004_000951"
     CDS             5085..5390
                     /locus_tag="PW004_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725939.1"
                     /translation="MNVQVYLKRNLARFPRSWDTNSHPSEAEEESYLMSANMIEITRD
                     DADEFDKKRSWYSVSNPMYTAVIEDYHISDRFILVDLERPKKRIKQYRRCGFSTKKW"
     gene            5414..5617
                     /locus_tag="PW004_000952"
     CDS             5414..5617
                     /locus_tag="PW004_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HDL2725940.1"
                     /translation="MNNRHRRITKLRKQELNVLKTKFEKEYGVSAEETYKVASQCVAD
                     ASDAIRKFGISILSDDRKWEEKE"
     gene            5614..5910
                     /locus_tag="PW004_000953"
     CDS             5614..5910
                     /locus_tag="PW004_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725941.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMQTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKLLAKSKENLAVVRLVDIELINDK"
     gene            5986..6402
                     /locus_tag="PW004_000954"
     CDS             5986..6402
                     /locus_tag="PW004_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HDL2725942.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLIQYR"
     gene            7310..7654
                     /locus_tag="PW004_000955"
     CDS             7310..7654
                     /locus_tag="PW004_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDL2725943.1"
                     /translation="MTEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            7659..7940
                     /locus_tag="PW004_000956"
     CDS             7659..7940
                     /locus_tag="PW004_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725944.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            8043..8357
                     /locus_tag="PW004_000957"
     CDS             8043..8357
                     /locus_tag="PW004_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HDL2725945.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            8335..10029
                     /locus_tag="PW004_000958"
     CDS             8335..10029
                     /locus_tag="PW004_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDL2725946.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKNR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNANLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            10049..11227
                     /locus_tag="PW004_000959"
     CDS             10049..11227
                     /locus_tag="PW004_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDL2725947.1"
                     /translation="MGIFQKAVGYFTKKATVTLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            11190..11876
                     /locus_tag="PW004_000960"
     CDS             11190..11876
                     /locus_tag="PW004_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HDL2725948.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            11876..13036
                     /locus_tag="PW004_000961"
     CDS             11876..13036
                     /locus_tag="PW004_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDL2725949.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            13046..13921
                     /locus_tag="PW004_000962"
     CDS             13046..13921
                     /locus_tag="PW004_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725950.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSNVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            13918..14229
                     /locus_tag="PW004_000963"
     CDS             13918..14229
                     /locus_tag="PW004_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725951.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            14219..14572
                     /locus_tag="PW004_000964"
     CDS             14219..14572
                     /locus_tag="PW004_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HDL2725952.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            14562..14963
                     /locus_tag="PW004_000965"
     CDS             14562..14963
                     /locus_tag="PW004_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725953.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            14956..15360
                     /locus_tag="PW004_000966"
     CDS             14956..15360
                     /locus_tag="PW004_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725954.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            15372..15980
                     /locus_tag="PW004_000967"
     CDS             15372..15980
                     /locus_tag="PW004_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2725955.1"
                     /translation="MAKTKTVVTTFDNISIKRIAFNFKNAGNAIVTNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            16000..16362
                     /locus_tag="PW004_000968"
     CDS             16000..16362
                     /locus_tag="PW004_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725956.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMSM
                     GKGSMDITVIQGAKAVYVAYRQANMVDYISFDEFIDKWDFDMEVAVAVYSTMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            16365..16547
                     /locus_tag="PW004_000969"
     CDS             16365..16547
                     /locus_tag="PW004_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725957.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            16565..19897
                     /locus_tag="PW004_000970"
     CDS             16565..19897
                     /locus_tag="PW004_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2725958.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNDIDKTSKALAQSKGEFKTYESTMKDLDNEQQHLKSSASLVESEYKKWQ
                     ATAGQSATESEKLAKAQEYVGKQSDIAEQKIEVLKKQLDATQKEFGETSTEALEMKTK
                     LNDAEREFEELSNAAKNVDTSTVDDIGKKLDMGNLMEATDHLSVIGDKLIDVGSKSIE
                     AAGKAQAMQAQFKQVFGSLEGEAQDAVEGMAEEFGMLPNTIKPVFIQYTSMFKGLGYD
                     TKEAMELAGDSTQLAADAAAFYDKSMDDASESLNSFIKGNYEGGEQIGLFANDTQMAA
                     YAVKHNLIPATEGAKKASEESLLAVEKAQSKYADAIKKHGEGSLEAREAALKLKDAQD
                     KINEELGPQTQKWSDLDEATKQAVRVQYAEDMQKLAGATGQASRESDGLENQMTRAKQ
                     ALEDFYASLGEDILPVFIKGLQAGAKALQGLAEWWSKLDGPMKNFILALGGILALLST
                     LAPVITAVVTIVGTFGSTVLLPIIGIIAGVAAVIAIVITAFQNWGAITDWFSDLWKKF
                     TDWLGDTWESIKESASSVWDGVKETWSGFVDWVQEIWQGVSDWFGELWSGLVEGASNI
                     WQGVQETWQAFVDWVSNIWNGVKEVWSIIWADIVGIVQIPWTLITSLIQAGINIIVGI
                     FDVAGQLLGAAWQAVWTPISDFLKNTWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNG
                     IWTSISTTASNIWNSISATASSIWNSIKNTITSLVQAAATVIQNIWSTVSSWLGGIWN
                     SISSTASNIWNSVTSSISNAINAAKSAIQSVWNSISSWISGIWNGIKNTALNLWNGIT
                     STISSKVNDGKNAISSGWSNLTGIVSDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKI
                     SGIFDNLNISIPHIPLPEFIMEGSFNPLKGQIPHLRVKWHAKGGIFTKPTLLGGMNGV
                     GEAGPEAVLPLKRSVLQEIGDRILSSTSVSSRAQTIQPVNNYEFNFTIDGNADEVTMK
                     QTTQQIIDSITKIQNDNASAWR"
     gene            19948..20685
                     /locus_tag="PW004_000971"
     CDS             19948..20685
                     /locus_tag="PW004_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2725959.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            20695..22986
                     /locus_tag="PW004_000972"
     CDS             20695..22986
                     /locus_tag="PW004_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2725960.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYDTAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYINDGDGNSLGIDGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDVDHFIIETSKPNVLVRWNLVAHRL
                     GYEDVRLKEDTAYDSTVLDQKRF"
     gene            23010..25091
                     /locus_tag="PW004_000973"
     CDS             23010..25091
                     /locus_tag="PW004_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725961.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDDQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFLAEIKGRMDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLHVMYSNSIDFGGYDYSGNPNLMSKLKSSDFN
                     VGYHGSLTNVNGMLHFMSDGTGDIDMFTRINTPQLASGKTYTLSAKVRFDEGTTGAIN
                     KLRLVYRTSPGENILLEANNTTITIDDVGKEITIKGTANVNYQITNLDRFYISVSFSN
                     RDKINGGFKLYDIKIEEGSTATPYQPNLLDDPYWVGKTSLGKNIANKGTTFPIKSSAY
                     EIYKGNTEEELIIGQTYTITLKGTKPASQTFVAYNYWNVNFGDLKPVEGLTDVWSLTF
                     TPTKLEPGLPKDLRIFQSPKETAGACRIDWLKIEKGDTRTPNIEQYKYRGIGMRDSNN
                     PKDYVWDLEPSYVENNLATDIKVAEITGKANNYTDGKVSEINSQLTASINEVDTTAKD
                     AQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFVALN
                     GKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYG
                     SAYSFVITYSI"
BASE COUNT         9059 a         3773 c         5420 g         6976 t
ORIGIN      
        1 gtacagcaac gaaacttcat ctatgcgctg ataggcgata ttttcatcga tacaggcatg
       61 ccaacggact tctggaagga attcttctac ttccgtttcg aaggtgtcac agggcgcaaa
      121 ataagcctca aagatgaatc gagcacaacc gtaagtgatg ccaatatctt agcgaatatc
      181 atcctagatt tcatctttga gcatgatatt ccgtttaaaa atggatatga aattttgccc
      241 caaaacgaac agtatttctt ttacaaatgc ctcacgaaca gggtgtgctg tagttgcgga
      301 aagaaaaatg ctgatataca tcacgtagac tctgtcggca tgggaaataa ccgaaaaaaa
      361 ataaacaatt ctggtaggcg ctttatggct ctgtgcagag aatgccatac gaaaattcat
      421 gtcgaaggtt ttactacatt tacaactaaa agaaagctta cggcagttgt tctcaaagac
      481 agtgatttaa aaagattggg gttgaatatc attgaatgaa ttatggatgg atatcgaagg
      541 atacgaggga gtatatcaag ttagtagctt aggaagagtt aaatctaatc gccctaacta
      601 tgtaattaat aagggatacg ttaacaaaga aggatttatt atgaaaccat ctgacaatgg
      661 gaaagggtat caaattatct tcttaagcgg caaaggtttt agagatagaa ggtatgtcca
      721 tcggctagta gcttttcact ttttaaaaga agcgtatttc gaaggtgctg aagtcaatca
      781 taagaacgga gataaatcta acaatactgt tgaaaaccta gaatggctca gttctgatga
      841 taataaaaag catgctagaa aaaatggatt aactaacttg aaaggaccat caaaactaac
      901 tgatttgcaa gctttagcta tcaagcgttt atatacaaac aaattgatga gctccggaga
      961 aataatgaaa ctttttaatg tcagcaggca taccgtgctg aatatagctt cagggaagac
     1021 atttagtttc ttggaagatt aagagaaaca caaaataggt gtgactaatt tcaaaaataa
     1081 atatcaaatc aaagggatca agttaaacca ggaaacaatt aaaaagttaa ggataggagg
     1141 ataaatttgg ctgagataag ttggatcaaa cttaaaacta ctatgtttga cgatgaaaaa
     1201 atacgattaa tccaagctgt tcctgagtcg gatgccatca tcgttatatg gattcgatta
     1261 ctagttttag caggaaagac taacgacgat ggtctgatat atatccagag gaacatgcct
     1321 tataccgaag aaatgcttgc tacattgttt ggcaaaaacg taaatacggt tcgcttagcg
     1381 ctaactacat tggcaaattt caacatgatt gatctaagca gtgatggact aattgccatc
     1441 agtaattggg aaaaacatca aaatatcgag ggtatggata aagtaaggct aaaaaatgct
     1501 gaaagaaacc gtaaatacag ggaaagaaag agacaggaac gtctcaaatt ggaaaatgac
     1561 gttagcgtga cgtcacgtga cggtacagat aaagatatag aagaagataa agatatagat
     1621 aaagaagaaa agaaaggtaa gtattctgac gaacacttac gccttgctaa aaagttgcaa
     1681 agtaatttaa ctgaagattt tccaaaagaa atgaacaaag tagatatcga aaaatgggca
     1741 gacacaatca ggttgatgga agaaagagat aaagcatcta tagaagcgat tgagtatgtg
     1801 atcaattggc tacctacaaa tgaattttgg tttggaaata ttagaagtgc taagaaattg
     1861 agagaaaaat ttgagaagct caaattcgaa atcaaagcag acaagaagaa tcataaaaag
     1921 caaagtcaaa aactacagta cagcaatcct agtgaatatg acgacttgcc aatttaaaaa
     1981 ggagatgcat cacatggaaa gcctagcaaa tgctatggag aaactaataa gaagagtatt
     2041 agtgcaaagc ggaaaatgtc cagaatgtag cgaacctttg tatagttggc gagctaaaaa
     2101 taaggatggt tcagaacgtt gtaaaccaac atgcatgagt tgtggttata aagcgttacg
     2161 tgtgaaagag gatatacaga ccgaacggat atataacgat agcttaaaag cacgagcgtt
     2221 gagttttttt caaaatggtt cggtattaac agataaaact ttgtttaaat gcaaaatgga
     2281 aaattatcac gtagtggatc aagaaacaaa aatcgcttta gaaaaagcaa aaagctatac
     2341 gaatgaggtt ctgctgaacc atcctgcaca tttcattcta tcagggaaat caggaagcgg
     2401 aaaaagccac ttatcaatgg ccacagcttg ggaaatactt gagcgctcaa attatgacaa
     2461 gaaaatactt tttataagct atcaagaatt attagagcaa ataaagtttt cttataacaa
     2521 tactgaactg agaaaagaaa ttgaaggatc gcttatagcc gatattaaaa caactgattt
     2581 ggtggttttt gacgatattg gagctgaatt aggtagtggg gtatcaaata gtaggcagtt
     2641 tacaaacaac acgttaaaca cgctcttaga agccagacag aacaaggcaa cgatcatcac
     2701 aacaaactta tctggtcctg aactaagaga agcctacggt gaaagaattg tttctaggat
     2761 atttaagaat tcagaaggtt atgcgctgaa attccaacaa acagcagaca agcgcataaa
     2821 accagtgaaa ggtagtatcg catgaataaa taccgtaata aaaaaactgt tcatcgaggt
     2881 atcaagtttg attctatcgc agaagcagag tattacgatc tagccttgtg gcaagctgaa
     2941 gcaaatggct ggaaagtaaa acttcaggaa agatttgagc tgatgccgaa atttgaacta
     3001 gacggaaaga agtatcgcaa gatcgagtat attcccgact tcacatttta taaaaacggc
     3061 aaacttgtca aagtcgtaga tgtcaaagga atgcagacaa aagactttaa gatcaaggca
     3121 aagttgttct gtcatcaata tcaagcgccg ttgatattag ctaaaaaata tcggaatacg
     3181 ttcaaggaag agcgttttta acgaggtggt ccatcatgac aacagaagaa gtgattcaaa
     3241 tgcgtattcg aaacattcag cgtgaaattg acgatctaga acgaacaaag gcagtgatgg
     3301 tcaatgaaac ggcgagaaag gcaatcgatt tgcacataga gaacttaaga agggaaattc
     3361 gtagattgga ggaatgagcg tggataagaa agcgacaatg aaacgaattg ctgaattaac
     3421 caagtcagaa tcttggcagg aagacaaaga aatagttgca gaagtccaaa agctcggtaa
     3481 atcaatgtgg actgaaaagc ctaaacggaa aacgccgaga aaaattgcaa tctggcatgg
     3541 tgatcgaatt ctagtaacag gtacagctga acagttatct gaaattactg gattaagcaa
     3601 aaacattatc tgggatagag ctaggagctt atggattgat tcaaaaggac gacagtttag
     3661 gtatgtggag gagagataat ggatctcatt acacaataca gtgacatcat cctcaagaaa
     3721 atcatgatga agattcagaa agacaaaaaa tcaaaagaac gagcggaatt agttaagttg
     3781 gaaatggctg aaacaggagc aggagtgcga agtagcaggc attggaaagc agcagcaaac
     3841 attgaatttt attacaacga aattcaaaaa gggttcgatc agatgcgtga gctggatcgg
     3901 caaacaaatt ggagcaagaa acttcatcaa gatcgtttca agtttgtaaa gaaatataaa
     3961 gagatattag acaaatacat ggaggacagc aaatgatact gaagtacagg gcatggaata
     4021 agaaaactca gtcatttatt gattatggtg atttggtttt agatttgaga agcggaaaaa
     4081 tttacgctgg ggacattggg ttagtggaaa gcaccattga tgtgactgac caaattgaac
     4141 tcatgcaatc cacaggaatg aaagataaga atggtgtgga gatatttgaa ggggatgtag
     4201 taattacatc aaaaggagcg attggatatg ttgcgtatct tcaacaagaa gccggctttg
     4261 tagttgtact aaagaaaagt gattatagat taggccatag gaatactggc gaaagttatg
     4321 atgtagcaac tgctcatgaa gtcatcggaa atatatacga gaatagcgag ttattggagg
     4381 tagagtgatt atcttagcta ctgactacat cgattcttta aaagatgaaa atgggaatgt
     4441 cccagcattg ctaaattgtg gaattaaaag caagaataga atcattttgt tacggatatc
     4501 ggctgattta gcaaaatatc tttatctgag caagacagca gtatattaca ctgcggctac
     4561 acgttatcag tacaagggaa aaacaatcag tcaggattat tatgatcgtt taatcagtcc
     4621 tgacatgcat ggaaaatcaa aatcagcgat caagttattt ggcgcaatag agatatatgc
     4681 cgatgatttt ccaaatttat ggttgaagga ggaagcggaa tgaacgagga aataaaaaaa
     4741 tctattcgtt acattgatag agcgttatcg caactcgaac ccttgcgaat tgaaagtcat
     4801 aaaggaatga gtgtctaccc aaatcagtat gacaaaatga acgctgattt acgtaatgca
     4861 aagctggcct taattaacat taaaaataac aaaggctgcc agtattgcaa acacagcgaa
     4921 ccgttgaatg ataccagcaa ctctaacttt gcaactgcaa ttgagtgtga ggaaaatgga
     4981 tactacattg caactgagta tgacgatggc gattatgcag ataatggcta ttctgcttat
     5041 atccaatatt gccctatgtg cggtcgaaaa ttggaggtag cggaatgaat gttcaagttt
     5101 atttaaaaag aaatttggcc aggtttcctc gaagctggga tacaaattcc cacccatccg
     5161 aggcagaaga agagagctat ctaatgtctg ctaacatgat agaaattaca agagatgatg
     5221 cggatgagtt tgataaaaaa agatcatggt actcggtttc gaatccaatg tatacggctg
     5281 taatagaaga ttatcatata tccgacagat ttattctggt tgatctagaa agacctaaga
     5341 aacgaattaa acagtataga cgttgcgggt tttcaacaaa gaaatggtga aaacaaaaca
     5401 gaggtgactc agaatgaaca acagacatcg cagaataaca aaactaagaa aacaggaact
     5461 gaatgtacta aagacaaagt ttgaaaaaga atacggagtt tctgcagaag aaacatataa
     5521 agtggcaagt cagtgtgttg ctgatgcaag tgatgctatt cgtaagtttg gaatttcgat
     5581 attaagtgat gatcgtaaat gggaggaaaa agaatgaaac taaaagacgg attttacgct
     5641 agtagccacg gtatcggcgg tttaatgctg gatatgcaga caaagaaccc taaaacacgt
     5701 aagaaaccaa aattcaaagt cggtgacatg gttcgctgcg aagcagaagg gttcatctat
     5761 ccatttcgtg gatatgtaga acatgtctat aatcactcag cgatcattcg cattgaaaac
     5821 acgatggaat gcgacaagtt attagcgaaa agcaaagaga atttagcagt ggtgagattg
     5881 gtggatattg aactaatcaa tgacaaataa aaaagccgga tcgctccgac taacataata
     5941 aaacagacaa gtttattata tcacataaag gagcggtttg acttgatgca attgttacga
     6001 gaggtagatt tcaaacagac aagatgtaat gcgagagatg tgctgaagaa ctttcggcgt
     6061 ttggagcgta tggcaggtcg ctctttgata gatattaagt cgccgattat aacggatatg
     6121 ccgaaggcgc cgaagcacgg caataaggca gaggacgcga tcattcagat gatggatata
     6181 gaagcagaga gagatgcgat tctagcggct ttgatggctc ttagtctgat tagtcgtcag
     6241 atactctact acagcttctg cgacgtaaat aagcattcta attatgaaat aggtcaattg
     6301 atacgaggat acggagagaa gaatgtagag aagttgaaat ccatcgcatt gattgaattt
     6361 gctgaagcat ataaaaaagg cgtattaatt cagtatcgtt gattttgtag ggtttttgta
     6421 gggatagtgt agggtttttg agcggtttaa cgtgatatta tggtagtgtc gaaagattag
     6481 gaaacaggac ttcgacaaaa taaaatgtaa gggaggaaat ctccctcatc gtttaattaa
     6541 gcttcgatag acagcagcgg aaatattaag aataaggatg tgaattttaa ctccttctaa
     6601 attgttctta ttatctatca tccgttgctg tctattgtca ttatgtcact gtggcggaaa
     6661 gggtagacgc ttaaaaataa ggtcaatacg tcgagggata gccttaacgt tttatgattt
     6721 gaccatgcaa tgttcgattc attgccagtg acttaaggaa cctacggaaa caattcatct
     6781 tatcggatgc cgatgaattg gctgactagt cgggatgcta ctagcagtta gaaggcataa
     6841 aatactagcg cagacgtgcg ccactctcag gtgtaggtta ggagagaaac attagttggg
     6901 gttattagga atacgataac ctgcttgcga caaagctttg tactgtcgcg ttggtcatga
     6961 acagagacgg tattctgttt cagtattcgt tagcaaccga gggatgtggc agtggtgagg
     7021 tgcaggaagt atcagacttg tttgtgtgca agttactatt acatattaga tcactctttg
     7081 agtggtcttt ttattttgcg taaaggaggc acgacgatgt atagaccaca atacttagaa
     7141 cagaagcatg aagtaatcac tgtgcaaaac ggtaacggtg agatagtacg aaagtataga
     7201 agaccaataa agagcgatac atataaacgg aaggaaagca atgaagttat tccattgtat
     7261 ggcaaaagaa tagctaagta ttaaataaga ttgcgaaagg agatgggaca tgaccgagga
     7321 attctataga tggttattac agttgataag agaagatcgt ttggttaagt tctatcagtc
     7381 tcctaaatgg cgcaggctta gagagaaagc gatgaaacga gatcactatg aatgccaaga
     7441 gtgtagaaga ctaggtaagt atcatagagt agagaacgtt catcatataa aggaagtcaa
     7501 ggatagacct gacttagctt tagatttaga taatcttatt tgtttatgtg ttgaacatca
     7561 taatgaagtt catggcagat atcttacagc gttagataaa caagagaaga agatagaaag
     7621 cttcgctaac ttcgatgcaa gtgaaaggtg gtaagtgcat gatcatcaat gacaatggca
     7681 gagagtatga tacagaaaag attgaagagt attcatctta tactcaggga ttaattaaac
     7741 gtttgatata cgttcgctat gtaggtatta gggatctgtt atcagataac tgttgtagta
     7801 aatacaaagt gaatcaagta agagaagcgt tgaataaaga taataacgtc gaaagaataa
     7861 aaaatgtttt tggatatagt attgaagaga ttaattatta cattgacttc gctgaagctt
     7921 tcattccgat ggtgagataa ccccccctta aaataaatcg caaatttttt gggggtgatg
     7981 aaacggaggg ggctgtcagg aaaagagatt ttttcgaact ttatcatgaa aggagggcta
     8041 aaatgtttaa aaacgaattg tctcaaaatc gctacagaga aaaattacgc cgctctttaa
     8101 taagccaatt ggaaagtcag aaaacaaata ttgagccatt cttagataat gttgatcgtt
     8161 atatcagttt atgggaaacg gcgatatcac tggaagaaga tatatccgag aacggcatta
     8221 gactggagaa tggtaaaaag aatgaatcag tagcgttgct tgtttctgtc aacaaacaaa
     8281 tgggattgat gttggataaa cttgccatta ctcctgaatt ggtaggtgaa gcaaatgaat
     8341 caattcctga gttataagca tattgaaaat tggttcaaag ctatagaaga aggcactatc
     8401 aaggtatgca aagagcaatt attgctaaaa aattatctag aagaaagagt ctttactaga
     8461 gaagatattt acttcgataa gcagatggta gaggattcaa tcaatatacc agcacaatac
     8521 tttccattcg aattaattcc gtgggaaaaa tttctacaat gttttattta tggtgttcga
     8581 tggaaaaaag ataaaacact agtgttcaat agatatcttt cattaatggg acgtggtaat
     8641 ggtaaaactg gttttgcttc ttggaacaac ttctttctac taaccgctaa acacggtatt
     8701 aaaaattatg atattgatat ctatgccaat aatgaaagcc aagcaaagac tagttttgat
     8761 gatgtattta aagtaattaa agatcatcct gatttagata aaaaagtatt taaagctacg
     8821 aaggaagtta ttcaaaatat cgctacaaac agcaaacttc gttataacac ggcaaatgct
     8881 agaacaaaag atgggaagcg accaggtgca aaccgctttg atgaaattca cgaaaatgaa
     8941 gattattcaa tgataaatgt ggctacttct ggtggtggta aaaatcgaga ttatagagaa
     9001 ttttatgata caactaatgg tcatgttcgt ggtggtccgc ttgatgacat tatagaagaa
     9061 tcaaaaatga ttctttctgg agaacttgga attgacaagg atggagcaga attttctagt
     9121 ttgtttccat ttatttgtcg cttggataac gataatgaag ttgatgatcc cgacatgtgg
     9181 gaaaaagctt gtccaactat taattacaat gcaaatttaa aacggaaaat gtttcaagaa
     9241 tactctcaaa tgcaacgtaa tgctggttta agacttacgt tcatgaccaa acgaatgaac
     9301 agacctatgg aagatacacg atttgctgtt gcttcatatg atgatgttct gcatacgaaa
     9361 gaaaaagaat ttcctgaaaa aatggatgaa gtgataggaa cagtcgattt tgctgataga
     9421 cgagattttg ccagcgttgg gttgctagga aaatacgata aagatgttta ttttacacaa
     9481 catactttta tccacgaatc agcccttcga ttacaaaaca tcaaacgaga ggttatagat
     9541 atttctatag atcaaggaaa atcacagatc gttcatggaa aaaatataga agctgattat
     9601 attgtaggtt ggtttcttga aatgagtaat aaatattata ttaaaaaaat cgctatggat
     9661 atgtaccgtg caaaaatatt gaagcccgct ttagaagaag caggttttac tgtggaaatt
     9721 gttcgaagcg gatctgttac acatggtatg ttaaaagatc tggttgatga cctttttatt
     9781 aatcaacgtt tattttttgg tgacgatgcg attatgcgtt ggtattgcat gaatgtatat
     9841 gaagagcata tttctaatgg aaatatacgc tatgaaaaaa tagaacctga aactagaaaa
     9901 acggatggct ttttttcatt ccttcatggt ttgaattttt tagatgatat ttatgattct
     9961 gctcctgtaa cagtcacaaa tagctcagta gaaaatacag gaactggatt tactcctcta
    10021 gtattctaac ttgaaaggag gtgagaaagt ggggattttt caaaaggcgg taggatactt
    10081 cacaaaaaaa gcaacggtta ctttagaaga atacttttgt aaattgcaag ttgattttgt
    10141 gtatcgaaaa tttgcgattg aaacttgtat tgatttgatt gcaaatgcga tgagtaaagc
    10201 ggaattcaag tcatatgaag atggaaaaaa taaaaagaat gatctttact ataggctgaa
    10261 tgtagctcct aataagaaaa ataatgcaac agaatttaga aaaaaactga tcaggagatt
    10321 aatattctac aatgaagtat tgatcgtttc tccgtctaat aattctagcg aaatatttat
    10381 tgcggatagt tgggatgtca cagaatatgc attgaaagat gatgtgtttt ctcaagtgca
    10441 aattaacaac atagtccttg atagagaatt tctagaaagt gatgttatct atataaaata
    10501 cgcagatcaa caaattaggc aactagtcga tgcgtattat caagcgtatg ggaaactcat
    10561 ttctagtgcc atgaatgttt acaagcgctc taacgctcgt agatacgtac tgaaagggaa
    10621 tttattccga ccgcaagaca atacaacaca agatcaaatc aataaaatga tgacatcaca
    10681 atttaaggct tttatggaag ctgataatgc aggtgcggta tttcaattac aaaatgagta
    10741 cacattagaa gatttcagcg gaaacttcca aagcaattca agagatataa aaaacttaat
    10801 agacgacatc tttgagatga cagcagcagc gtttcacgtt ccgaaaaacc tactaaaggg
    10861 agacatgagt gggttatcgg atcaagtgga cgctttttta atgttcgaaa tcataccgat
    10921 tgctgaactt attcaggatg cgtttaacgc tagtctctat gaagtagaag aatacttgtc
    10981 agggaatttt gtacgtgtgg atacaactat gatcaagatt actagcttca aagatttggt
    11041 tgacgctatt gatgtaggca ttagaaatgg agtatttaca atcaacgaag gaagagaacg
    11101 cgttggaaat gatcgctctg ataaggcgat ggcagatgaa atatttataa ctaaaaacaa
    11161 tcaacaagta tcgaaaggag gtgaggcgaa tgacgacaat gaaaacattt ctagcagtaa
    11221 agaatgaagg cgcagtaccg caaattttta ttcagggatt tattggttct agttggttct
    11281 ttgaagggaa tactgacaag ggaatcaaaa atattttgga tagtctaggt gatcaagaag
    11341 aaattgaagt agtaattaat tcaaacggtg gagacgtatt tcaagggatt gctattggga
    11401 acttacttaa gtcaaataaa gcaaaagtta acgttgtgat taacggctta gccgctagtg
    11461 ctgcttcaat tatcgcaatg gctggcgata ctataaaaat ctacaacaat gcacaattga
    11521 tgattcaccg cgcttccaca tacggagaag gaaatgtcga tgacttccgt acgattgctg
    11581 accaactgga atcaattgat aaatcggtaa aggcttcata taaaacacga ttcaatggca
    11641 cagatgaagc attgcaagaa cttcttgaaa aagaatcgtt tatggatgca gaaacagctt
    11701 tgagttatgg attggtcgat gaaattatcg atgcagaaaa tagctcaggt actgaagcta
    11761 aaaaagaaca aagcgttgaa gaaattttga atgacgttaa agaaaaaaga gcagaaaaaa
    11821 ttgctgcatt tacagcagca ttaaataaaa catttggaca aggagatgta aaataatgac
    11881 agttaaaaat ttaaaaggtg taacagctgc aagcgaccaa ttgatgaaag cttttaaaga
    11941 tggtaacgaa gaatctttta gcgcagctat ggtaagctta tctaaggaaa ttcaggataa
    12001 aattttagaa gaagcaacag caaaaaatca agatcaatta gtattaatga accgtggtca
    12061 gcgtgtatta actacacaag aaacaaaatt ctataacgaa gtagtgaaaa acgaaggttt
    12121 tgcaggggtc gaagaattag tgccagctac tgtatttgaa cgtgtatttg aagatttaga
    12181 acaatctcat ccactattgc aaaaaattac ttttgttaac acaactggtg taacagaatg
    12241 gattgtgtca cgtggagtca atccagcatg gtggggtaaa ctttgcgaag ctgttaaaaa
    12301 agttttagat aatggctttg atgtaattaa catgaagcag ttcaagctat caggttatat
    12361 tcctgtatgt aaggcaatgc ttgatttagg tccagtatgg ttagatcgtt atgtccgtac
    12421 tgttttagta gaatcattga gaattgcatt agaacaagca attgttgatg gtactggtaa
    12481 agatatgcca gtcggaatga tgcgtgacat gagcaaacaa actagcggag aatatgctga
    12541 aaaaaaagca gaacctatta cagctttaga tgctgtaact atgggcggtt tgatggcgcg
    12601 actatcaaaa ttcaatatcg aaggtgtgaa tgatccgatt tatcgtaatg tgaatccttc
    12661 tgatgtggtc ctaattgtga atccaactga ttactggtct aaagtattcc cagctaagac
    12721 tgtactaact gctaatggag aatacgtaca agtattgcca gtaccagttt cagatttgca
    12781 gtcaacggct gtgccagaag gaaaagcagt tattggggta gcctcagatt acttcatggg
    12841 tgtaggatct acactaaaaa ttgaagcttc agatgaatac cattttgttg aagacgaacg
    12901 catttatcta gctaaacaat atgcaaacgg tcaacctaaa cgtaacgata gtttcattgt
    12961 attagatatt agcgctttgg gaactactac tacaactaca aaaccaacaa ccacaacaac
    13021 tacaacacaa gcgtaggtga tcagaatgaa gtatattctt tgtcagccgg caatcaatcg
    13081 gtttaaatgg gagcttgaag tttgtttaac taatctgaag aaactaggaa tcaaagatat
    13141 cgtattgctt ttcagcagac acgatgatca gattcctatt ttttttgaga aggaatatgg
    13201 tgttgaagtt catgtgtacg atgatctgcg ggacgacaaa gagtatattc cttcgattaa
    13261 accatattta tggtggaaat atttagaaga agatcattcg cgtgaggacg accgatattt
    13321 ctatatcgat tcgaatgtca ttttcaataa aagaattaat ttgcgcaaat tgccttctaa
    13381 agatgatgtt tggtattgta gcgactgctg tagttatcta agtcttgatt atattagaag
    13441 ctgtgaaaac ggagaaaata ttctaaaaga tatggcaaac attgtaaatg ttacagtaga
    13501 atctttggaa actataaaca ctaattcagg aggcgcacag tgggttatta accgtcctaa
    13561 agcgaattat tggaaaaagg tttatctgga ttctaatcgg ctatatcgct accttagagg
    13621 gcaaaaaaca aatatacaaa tctggacagc cgagatgtgg gcacagcttt ggaacatgat
    13681 gtatttcaat attggtccta aagttcacga ggaattagac ttttgttttg ctactgatcc
    13741 aatagaaaaa gttaaagaag taaaaatctt acacaatgct ggagtaacaa caaacgatga
    13801 agatttattt ttcaaaggga gatacgtgac ttctacgcct tttgatgaag atttatcatt
    13861 tgtaaacaag aaaaaatgct cttacgcata tgttaaagca attaaggcgg tggttagatg
    13921 acgcctgaac aagtgactga agaattgcta atagctgtga aggataatat ttacgttacc
    13981 tggaacgaag aagatgagtc aattaaaaag atgatagcta aaaatgctgt ttatcttcaa
    14041 agtaaagtga gtacaacact ttctttttct cctgaaagct tagaatacgg attgctaatc
    14101 gaaagatgta gatacgactg gaatcgtgct ttagatgagt ttgaacaaaa tttcgctagt
    14161 gagttattag gtttcattca acattatgcg ctacaagaat atattgcagg tgatgtgaat
    14221 ggcgaataat cgtagactcg aagaaacatt caacgatggt tggttaaaga ttttgacgca
    14281 aactaccaaa agaaatgaac taggaaaaaa gattggtgta gaagatacag aaatcacttc
    14341 tttaaaattt agaaatcttt ccatgagaga tagtgatata acagctatgg atgcgatggg
    14401 atcgaaatta actaagaaag taaagactcc atttcatcca atcgccaaga aatttaataa
    14461 agatcaatat tttatcgtaa tcgatagtat gcgttacaac gttatctatg ccgattacga
    14521 taatttttat atctattttt atcttgaaag tgtgggtgaa tatggtgatt gataattcta
    14581 aagaaaaaga acgtttaaat aagcaaattt ctgctatcaa aacttcctta gaagagcatt
    14641 ttggcctcaa actctttcaa gactccgtag gcgaggatga gctacctgat gattttaatt
    14701 acttcattct cgaaacagga gaaatagaaa tgatcactga gccaaaatat agcgtgggtc
    14761 aaaatctata tctaactttc tattcagaaa atagagaaga tttaacagga gactcactag
    14821 atattatttc attgattcaa aatcgttcga ttcgttttca gagaatggat cccaatcatt
    14881 taaaactaga gaaccaagat cgctatatcg atcaattggt atttacgttt agacgattat
    14941 tgaagagtga ttgtcatggc taaaaatagt tgggagctaa aaataaatgg acatgatgaa
    15001 cttcttgtgc ggatggaacg ctattcaagc gagagcgaac gactgattaa cgaagcattg
    15061 aaatcaaaag gttcggctat tgcagtggat aggattacag aaaaaattcc tgtttctgaa
    15121 gcagatttaa gaagagggca ccaacacgca aaaaatagtc gtccacttaa gactcaatac
    15181 attaatttgg gtttcatcat tagacctaca agaaaatttg agtatttaaa atatcctgat
    15241 ttggggatag gtacttctaa aagaaatcag ccggacgaat ttatgagaag aggattaggt
    15301 cttgcacttg atccaattac agaacttctg attcgtcaat tcgataaatt aaataaatag
    15361 ggggaacaac aatggctaaa acaaaaactg tagtaacaac gttcgataac ataagtatca
    15421 aacgaattgc ttttaatttt aagaacgcag gaaatgcaat cgtaacgaat tgtaatggac
    15481 aattagatgg cgaaacagaa atgcaaacgg tggttaaaaa atgtggagcg acagaagtaa
    15541 aatcaaaatc taaaccaatc aatatgacgg taacaattac tgcacatgta ccgatggaag
    15601 tttatcgacg tttcaatggg ttgaaacaag atggacgtat taaaccaggc atttactctt
    15661 acggtcctga ttccgtaggc gaagatttct cacttgctgc agagatcgtg gatgacttcg
    15721 aagaaaatag aaagttagtt ggtatgttag catgcacttc gaatacagga ttaacattct
    15781 ctattgaaaa tggtgcggat gaagtagctg cgttagaact agaaacaaaa gttatgcaag
    15841 atgaatttgg taaattttat catgaagcaa ttgttgcaga acttgaagaa gacttaacag
    15901 atcaatggat gacgaatcta tctgctgatg tgattaaaaa aacttcagtt gtgacaacta
    15961 cggccactca atcacagtaa aaaaaacgga ggtagcgaaa tgaacgaaga ttactcaaaa
    16021 attgaactaa acgatggaac aattttgaat ttagaaccta aactgaatat caagaaatta
    16081 ttgatgatca atagagattt taacacagac gagtttgcaa aaatgtcgat gggaaaaggc
    16141 tctatggata ttacagttat tcaaggtgca aaagccgtat atgtagctta tcgtcaagcg
    16201 aacatggtcg attacatttc attcgatgaa tttatagata aatgggattt tgatatggag
    16261 gttgcagtag ctgtatacag tactatgatg ttcaaacaag cacgtgatgc ctatcaaaaa
    16321 gaattcgaaa aagcaaataa ggaaaaaaag cttcaaaagt aaaaatgcca aagctcttag
    16381 ttgaaacgtg ggtcgatgtc tattcgatgt tgaccgacgt tttttctatg ccttcagatt
    16441 tggttttaag cgatatctgt ttagatgaca ttttgcaaat ggcttacaac aagagcgctt
    16501 atgaaggatg gaagaactat gcaataaatc aatcccagaa aaactaaagg aaaggaggta
    16561 aaaaatggct aaaaagagaa cggaagcaga agtaactttc atagctaacg atgacggatt
    16621 gaaatctacg ttaaaagaaa tcagtgctga attaactaaa aatagagcag aattaaaact
    16681 agaacaagct caattacaac agactggttc tgaatcagac aagttaggaa gtaaattatc
    16741 ttctttagaa aagcagtatg aattacaaag tcaaaaagtt gaagtaacta gtcaacgttt
    16801 agccaatgcc aaaaaatatt atggagaaaa ttccaccgaa gttcagaaac ttgaaagaga
    16861 actgattaat caacaaacag ctcagcaaag gttatctaat gatatagata aaacgagtaa
    16921 ggcattggct caatctaaag gtgaatttaa aacgtatgag tcaactatga aagacttaga
    16981 taatgagcaa caacatctaa agtcaagcgc atcattagta gaatccgaat ataaaaaatg
    17041 gcaagcaact gctggccagt ctgctacaga atctgaaaaa ttagcaaaag cacaagaata
    17101 tgtcggcaaa cagagtgata ttgctgaaca aaaaatcgaa gtgctaaaaa aacaattaga
    17161 tgccacacaa aaagaatttg gagaaacatc taccgaagct ttagaaatga aaactaagct
    17221 taatgatgcg gaaagagaat tcgaagaatt aagtaatgct gctaaaaacg ttgatacctc
    17281 cacagtagat gatatcggca aaaaacttga tatgggtaat ttaatggaag ctactgatca
    17341 cttgtcagtg atcggagata agcttattga tgtaggtagt aagtctattg aagcagctgg
    17401 aaaagcacaa gctatgcagg cccaatttaa acaagtcttt ggctctttag aaggggaagc
    17461 acaggacgcc gttgagggaa tggctgaaga atttggaatg ttaccaaata cgatcaagcc
    17521 tgtttttata caatatacgt caatgtttaa aggacttgga tatgatacca aagaagctat
    17581 ggagttagct ggtgatagta ctcagttagc agcagatgca gcagcttttt acgataagtc
    17641 tatggatgat gctagtgaat ctcttaattc atttataaaa gggaactacg aaggtgggga
    17701 gcaaataggt ttatttgcta atgatactca aatggcagct tatgctgtta agcataattt
    17761 gataccagcg actgaaggag caaaaaaagc cagtgaagag tcattgttag ctgttgaaaa
    17821 agcacaatct aagtatgctg atgctattaa gaaacatggt gaaggatctt tagaagcaag
    17881 agaggctgct ttaaaactta aagatgcgca agataaaata aatgaagaat taggcccaca
    17941 aacgcaaaaa tggtctgatt tagatgaggc taccaagcaa gcagttcgag ttcaatatgc
    18001 tgaagatatg caaaaattag ccggtgctac aggtcaggct agtagggagt ctgatggttt
    18061 agaaaaccaa atgacaagag caaagcaagc gttagaagat ttttatgcct cattgggtga
    18121 agatatactg cctgtgttta tcaaagggtt gcaagcagga gcgaaagctt tgcaaggatt
    18181 agccgaatgg tggagtaaac ttgatgggcc aatgaaaaat ttcattttag ctcttggagg
    18241 aattctagca ttattaagca cattagctcc tgttataacc gcagttgtta cgatagttgg
    18301 cacatttggt tctacagttt tgctgccaat aataggaatt atagcgggcg ttgcagctgt
    18361 aatagcgatt gtaattacag cgtttcaaaa ctggggcgca atcactgact ggtttagtga
    18421 tttatggaaa aaatttaccg attggttagg tgatacctgg gaaagtataa aagaaagtgc
    18481 ctcatcagtt tgggatggag tcaaagaaac ctggtctgga tttgtagatt gggttcaaga
    18541 aatttggcaa ggagtttctg attggtttgg agagttatgg agcggattag ttgaaggagc
    18601 ttccaacatc tggcaaggag tccaagagac ttggcaagca ttcgttgatt gggtttcaaa
    18661 tatttggaac ggagtcaaag aagtatggtc gattatttgg gcagacattg taggaattgt
    18721 tcaaatacca tggacattaa taacgtcatt gattcaagct ggtattaata ttatcgtggg
    18781 tatctttgat gtagctggac agttattagg cgcagcttgg caagctgttt ggacacctat
    18841 ttctgatttc cttaaaaata cttgggatac tatgacacaa tggataagca tcgcttggaa
    18901 tggaattgta actacattcc atactatatt tgatccagta gtggcatggt ggaatggtat
    18961 atggacatct attagtacta cggcttcaaa tatttggaat tcaattagtg caacagcttc
    19021 tagtatttgg aacagtatca agaatacaat cactagcttg gtacaagcag ctgctacagt
    19081 aattcaaaat atttggtcaa ctgtatctag ttggttaggt ggaatttgga attcaatcag
    19141 ctctacagca tcaaatatct ggaatagtgt gactagtagt ataagcaatg ctataaacgc
    19201 agctaaaagt gccattcaaa gtgtttggaa tagtatatct tcgtggatca gcggaatttg
    19261 gaacggtatc aaaaatactg ctttgaatct ttggaatgga attacaagca ctattagctc
    19321 taaagtaaac gatggaaaaa atgcaatttc aagcggttgg tccaatctaa caggtattgt
    19381 ttccgacata ttcaataatg ttaaaagtac aattgctaac atatgggaag gcatcaaaaa
    19441 gactgttagc gctccaattg attggattag agacaaaatc agtggcattt ttgataattt
    19501 gaatatttct ataccacata ttccgttacc ggaatttatc atggaaggca gctttaaccc
    19561 gctaaaaggt caaatccccc atttgcgtgt aaaatggcat gctaaaggag gtatctttac
    19621 taaaccaact ttactaggtg gaatgaacgg tgtcggtgaa gcaggacctg aagcagtttt
    19681 acctttgaaa agatctgttt tgcaagaaat tggtgatcgt atcttgagta gcacatcagt
    19741 ttcatctagg gcacaaacga ttcaacctgt gaataactac gaattcaatt tcacaattga
    19801 tggtaacgca gatgaggtta ctatgaagca aacaactcaa caaatcattg atagcattac
    19861 aaaaattcaa aatgataatg cttcggcatg gcgttaaaca ggagagtatt tctcctgttt
    19921 ttttagtatt aaaaaggatg tgaaaaaatg actgattgta tacattctat aatcgatgga
    19981 tttcctgatt atttgcataa attggcttta gctgaaagac caaccatacc ttctccaaaa
    20041 agacagagag ttgaaacttc tgttttaggt aggttaggtg gcttagtaca agattactcg
    20101 tttgaagata tgtcgtttac attgcactat aactatttag aggatgtgga agaccatcaa
    20161 gcgttcaagc aatcgtttta tatcatgcgt cattggttaa actatgcaaa gaaattagaa
    20221 ttctctgacg atcccaacgt ctattacgtt atccagacta tcgatattgg ggatgcagaa
    20281 aacgatattg ttgaatgggg agagttcgat gtaaatatta ctgcgaaacc attcgcaaga
    20341 gttcaagaag atgtacctat aaccgtagat aaaccacagt catttaactt gctgaataat
    20401 agtttagaag aaagttttcc aaagattatc atcactcctt cagctacttc atgccagttc
    20461 atcttaaatg attatgtgtt tagtttcgaa ggcttagtag caggaactga tgtagtcatt
    20521 gatagtgatt tgatgctttg ctacgaagag caatcggacg gagatatttt agatcggtcc
    20581 aacaaaatga agaccatgca atatccaacg ttgcaagtgg atattaatca ttttaattgt
    20641 actggtttga gcaaaataca aatttatcgt aacgggttaa ggtaggtgaa atagatgatc
    20701 gataatttaa taactattta cgataaaaat gacgcgaata atttagctga acatttatat
    20761 gatacgcaag gtttaggcgc tttgtcagac tggttaacag ctactgttag caataaacta
    20821 aacggagccg agatatttca gggtacttat tcaataaacg gaactaatgc agatttgatt
    20881 gtagaaggac gtattattca gtgttatgta gatgaaaatc gagcaaaaca gcgtctacgg
    20941 atttattatg caaaaacttc tgtaataggc aatacgatag aagtaaaagc tgaacctatt
    21001 ttcaatgata taagaaaatc ggtgttgaat aaatatgaca gcggaacaga aaagatcact
    21061 gctactcagg catggcaaaa cgcaaaagtt ttagcgaaac cagctatccc ttcgcagttt
    21121 tctttctcgt cattagtaga tacgcttgct aatgtgaaga tagaaaaggc gaatttttta
    21181 gaattctttg gtggaaaaga gggatctatt ctagatcgat ttcatggtga gtttctaaaa
    21241 gataataaca cattacgtca tgaaaaaagt ctaggtacgg atcataaaat caaagcgatt
    21301 tatactaaaa acttaactgg tcttgactta gagattgatg ctcaaagtgt tttagttgga
    21361 gtttatccat tcattagcag ctcttcagaa ggagaagacg agatcactct accagaagaa
    21421 gttattttca cggattacgt ggatgattat cctgctggat atgtttcttt tgttgatttt
    21481 aaagacaaag cgactgatgt agccacatta agggaagctg ctaaagactg gttgaaaaca
    21541 aacatagata aacaaaaacc acaagtgagt ggttcgattg aattagtacc attgaggcat
    21601 caaagaggct atgaaaaatt tgttgatcta gaaaaagttt cgatgggtga cggagtagat
    21661 gtgtatcatc cacagttaaa agtgaatatg tcagcgagaa ttgtggaata tacgtttaat
    21721 gttctaacca attcatacga taaattagtt gtaggaaacg tcaaaacaaa cttcttagaa
    21781 aacacagaga ataatgtcag caatttgatt aatgatgcca ttgatcaatt gaaaaatggt
    21841 ggcgaaatca gtgatttaat caatgatatt gtagatcatc aaactgatat gattactggt
    21901 caaaatggtg gttacgtttt attagatcct aaagaagccc ctagtcgtat tttgattatg
    21961 gacacaccag ataagaatac tgcacggaac gttttgcaaa tcaacaacgc tggtattggt
    22021 ttctctaaaa ctggcattaa tggaacgtat gacaccgcat ggacgttaga tggcggattc
    22081 aatgcctcgt ttattacagc tggtgagata gtagggatta ctattagagg tactacatta
    22141 attagtgatg gcactgatta tagaacaagt atagctaatg gcaaaatgac ttggtactca
    22201 aaaaaagtta acaaagatat tatggagcta gaagcacgtg attatgtaag tgctgatgcc
    22261 ggcattgtat catacaccat gaaaactggt ggtggtttca tgattagaaa tccacagggt
    22321 aacttggttt ttagtacgtg ggataatggt aataacagac cgtttttatc ttttggtgcg
    22381 ccaaatttca gatacagtaa tgctagttat ataaatgatg gtgacggtaa ttctttaggt
    22441 attgatggta gtgcaggtaa ttcatgggaa tttaaagtag ctggtaggac tatgaaattt
    22501 actagtgacg gtatgctaac gttgccaggt tgtttttttg gttcatggga agatgggaaa
    22561 cttgctagat ttgaacaatc aacggtacaa gtatataaag attttactgt tagaggtact
    22621 aaaaactcaa ccgtaccaac agaacattat ggacaacgac tattgaacgc ttatgaaact
    22681 ccagaatatt atttcgctga ttatggggaa gccgttacag gtgatgatgg taaagttcgt
    22741 gttgatattg accccatgtt tgctgagaca gtaaatctaa gtcggtatat gacacatgtg
    22801 acacctacag aactagtttt gtgtgctgtt actcatgaag atgttgacca tttcatcatt
    22861 gaaactagta agccaaacgt attggttaga tggaatttag tggcacatcg actagggtat
    22921 gaagatgtta gattaaaaga ggatacagca tatgatagca cagtgcttga ccaaaaacgt
    22981 ttttaaaacg aagacaagga ggtatataaa tggctagcag tttatataat ttggctttag
    23041 atttcagcaa agaattaaac tacaccaaag ctattatggc tcgtcaaggt gataaaggga
    23101 tcacggtgac tgttaaaccg tttttaaatg gcttgcagat ggatacgagc ggcggaacat
    23161 ttactttaaa aggaactacg ccatctaacc gttacgtaga tagtgtcgct actagtgtaa
    23221 ctagtgaaga agtcacattt tctcttgatg gcacatttat gagtgaagca gggtattata
    23281 aacactgcta tgtagaatat agaaaagacg atcaaatttt aacgacgcaa gatatcattt
    23341 ttttctcact aggagtgtct gacatttcgc aaggccaagc cgatgaatat gtttcgcaat
    23401 tagaagaatt gattcgaaag tataacgaaa cttttgatgc ttttctggct gaaattaaag
    23461 gtagaatgga tagcttaaat aaacagatta ctgatttaac tggtcaagct aaaacactac
    23521 aagacaagtt agatgctctg aaagaagaaa tttctaaatt aggtaactta catgtgatgt
    23581 actccaacag catcgacttc gggggctatg attatagtgg gaatccgaat ttaatgtcca
    23641 aactaaaatc gagcgatttt aacgttggtt accacgggtc actaactaat gttaacggaa
    23701 tgttacattt tatgtctgat ggtacagggg acattgatat gtttacgcgt attaatacac
    23761 ctcagcttgc tagtggcaaa acatatactc tgagtgcaaa agttcgattt gatgaaggaa
    23821 ctacaggagc tattaataaa ttacgtttgg tatatcgtac atcaccagga gaaaatatat
    23881 tattggaagc aaataataca actattacaa tagatgatgt agggaaagaa ataacaatca
    23941 aaggcacagc taacgttaat tatcaaatca caaatttaga tcgtttttat attagtgtta
    24001 gtttttctaa cagggataaa ataaatggtg gatttaaatt gtacgacatc aaaatcgaag
    24061 aaggctcaac agccactccg tatcagccaa acttactaga tgacccttac tgggtaggta
    24121 aaacatcgtt gggtaagaat attgcaaata aaggtacgac gttcccaatt aaatctagcg
    24181 cgtacgaaat atataaaggt aacacggaag aagagcttat aataggtcaa acgtatacta
    24241 tcacgcttaa aggaacaaaa cccgcaagtc aaacctttgt agcatataat tattggaacg
    24301 ttaattttgg agatctaaaa ccagttgagg gattgacaga cgtatggtct ctaacattca
    24361 caccaacgaa acttgaaccg ggtttgccta aagaccttcg catttttcag tcgcctaaag
    24421 aaacggccgg cgcatgccga attgactggc tcaagatcga aaaaggcgac acacgaaccc
    24481 cgaatattga gcaatataaa taccgaggaa tcggcatgcg agactcaaac aatccaaaag
    24541 attatgtatg ggatctagaa cctagctatg ttgagaataa cctagctaca gatatcaagg
    24601 ttgctgaaat cacaggtaaa gcaaacaatt ataccgatgg gaaagtatcg gagattaatt
    24661 cgcagttgac tgcttcaatt aatgaagtag acaccacagc taaggatgct caaacaaaag
    24721 cgaatgctaa tgcgactgct atagatgaat tagacaataa gatcgatgaa cgcattaatg
    24781 atacagctac taccacatta acagttacaa acgggaatac cggatcagca aagctttatc
    24841 gtgaaggaaa aacagtttct atatattttg tggctttaaa cggaaaaagc agtggtggaa
    24901 atgattcaac gatactaaca attccagaag gctatcggcc accaattagt tttgagcaac
    24961 tggttggctc gatagaccgt tctactttga acagtgctca actatctatt ggcgcagacg
    25021 gagccattaa atggagaaga aattcaagtt atggatcagc ttattcattt gttatcactt
    25081 attcaattta agggaggaaa tctaatgaaa gtagtttaca aatcaatcaa gccttacgga
    25141 ttcgagcaaa tcattttgaa caatcaagaa aatatccctg aaaactgtac agagattaaa
    25201 ccaccagttc ctaactggag accaagat
//
LOCUS       DANKKG010000028        24794 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000028 DANKKG010000000
VERSION     DANKKG010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24794
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.028"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            189..671
                     /locus_tag="PW004_000974"
     CDS             189..671
                     /locus_tag="PW004_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HDL2725962.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            708..1718
                     /locus_tag="PW004_000975"
     CDS             708..1718
                     /locus_tag="PW004_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HDL2725963.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(1833..2546)
                     /locus_tag="PW004_000976"
     CDS             complement(1833..2546)
                     /locus_tag="PW004_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725964.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(2736..3326)
                     /locus_tag="PW004_000977"
     CDS             complement(2736..3326)
                     /locus_tag="PW004_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HDL2725965.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            3810..4037
                     /locus_tag="PW004_000978"
     CDS             3810..4037
                     /locus_tag="PW004_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HDL2725966.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            4053..5219
                     /locus_tag="PW004_000979"
     CDS             4053..5219
                     /locus_tag="PW004_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HDL2725967.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNAHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            5453..6496
                     /gene="hrcA"
                     /locus_tag="PW004_000980"
     CDS             5453..6496
                     /gene="hrcA"
                     /locus_tag="PW004_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HDL2725968.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            6590..7153
                     /gene="grpE"
                     /locus_tag="PW004_000981"
     CDS             6590..7153
                     /gene="grpE"
                     /locus_tag="PW004_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HDL2725969.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            7204..9033
                     /gene="dnaK"
                     /locus_tag="PW004_000982"
     CDS             7204..9033
                     /gene="dnaK"
                     /locus_tag="PW004_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HDL2725970.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            9184..10350
                     /gene="dnaJ"
                     /locus_tag="PW004_000983"
     CDS             9184..10350
                     /gene="dnaJ"
                     /locus_tag="PW004_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HDL2725971.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(10432..11097)
                     /locus_tag="PW004_000984"
     CDS             complement(10432..11097)
                     /locus_tag="PW004_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2725972.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            11073..11246
                     /locus_tag="PW004_000985"
     CDS             11073..11246
                     /locus_tag="PW004_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725973.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(11291..12028)
                     /locus_tag="PW004_000986"
     CDS             complement(11291..12028)
                     /locus_tag="PW004_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL2725974.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(12030..13532)
                     /locus_tag="PW004_000987"
     CDS             complement(12030..13532)
                     /locus_tag="PW004_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HDL2725975.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13791..15785
                     /gene="uvrB"
                     /locus_tag="PW004_000988"
     CDS             13791..15785
                     /gene="uvrB"
                     /locus_tag="PW004_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HDL2725976.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            15795..18611
                     /gene="uvrA"
                     /locus_tag="PW004_000989"
     CDS             15795..18611
                     /gene="uvrA"
                     /locus_tag="PW004_000989"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDL2725977.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            18874..20139
                     /locus_tag="PW004_000990"
     CDS             18874..20139
                     /locus_tag="PW004_000990"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HDL2725978.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            20143..20874
                     /locus_tag="PW004_000991"
     CDS             20143..20874
                     /locus_tag="PW004_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HDL2725979.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            21009..21893
                     /gene="rapZ"
                     /locus_tag="PW004_000992"
     CDS             21009..21893
                     /gene="rapZ"
                     /locus_tag="PW004_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HDL2725980.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            21890..22888
                     /locus_tag="PW004_000993"
     CDS             21890..22888
                     /locus_tag="PW004_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HDL2725981.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            22922..23857
                     /gene="whiA"
                     /locus_tag="PW004_000994"
     CDS             22922..23857
                     /gene="whiA"
                     /locus_tag="PW004_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HDL2725982.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            24424..>24794
                     /locus_tag="PW004_000995"
     CDS             24424..>24794
                     /locus_tag="PW004_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2725983.1"
                     /translation="MCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIAEFIGSFRG
                     NVTTQPRRSWSIKLLNNSGEL"
BASE COUNT         8201 a         4241 c         5307 g         7045 t
ORIGIN      
        1 ttctttgatt tgattgatga tcggctgaaa tttttccatt ctaaaacgcc tccattaagt
       61 ggtttgattt attttaccag aaaaacaata gttatgtata aaaaacgaac aaaacacaag
      121 taatgttgta accgctatat ttatatgata agatgtctca aataataaaa tagaaaagag
      181 acataaaaat gattgacttg gcaaagaaat tcttacacga aaatatatat tttgagttgt
      241 atcatcatcg agccatttat acaaatgaag atgctttggt aataaagaaa gaacaaggat
      301 ttacaggtac agagaccaaa agcttattct taaaagataa aaaagataat aactacgtat
      361 atctgacttt tactacgaaa caaacggatt tcaaagcatt agggaagatc gtcggtaaaa
      421 ggctttctat tgtttcatca gaacgcatgg aagaacaaac gggacaaaag cagggcgctg
      481 tttctccgtt tggttatgaa aaacagattc cgattattat tgatgaagag ctcttatcaa
      541 atgaaaaact tgtttttgca ccaggtagac cagatcaaac gatggtcatc caaacggttg
      601 acttaaacaa aatcatccag ctacttgaga tgccgatatt tttgatgccg gcaaacgaaa
      661 aaaacaatta accaagtaaa atagtgcata cttggaggtg aagcaacatg ataagttatc
      721 cattattaga aagaaatgca acgattgggg ttactgcacc atcctcaggc gtatcagagg
      781 aacaccattc gttactcgaa tcagctttaa agagaatgga agaaaaatct ttccaagtag
      841 ttactggaaa gacgctttgg acgcaagata aggaaaaatc aggtccagca agaaaacgag
      901 cagatgaatt caatcaaatg atgcaagatg aaaatatcca aatgatcttt ccgacttggg
      961 gaggagaatt attgattgag attctcgatt ttgtcgattt tgatgctcta caaccaaaat
     1021 ggatagtagg ttattcagat attagtacgt tgctacttgc tgtcacatta aaaacaggga
     1081 tagccactgc acatggaact aaccttattg atttaagagg agagtacttg gatgaaacga
     1141 cagcaatgtg ggaaagtgta ctgaaaacaa gagaaggaga atcgattcta cagcaatctt
     1201 ctgaaaagta tcaaaaagtg tggccccatg agcattcatc atcttctgtt ttccatctca
     1261 ctgaaccaac aaattggcaa tcagtcagcg gagatgcagt aaggatgaag ggacgattat
     1321 taggtggttg tattgatacg atcaggcatt taatcggtac tccttatgga gatgtccaat
     1381 cttttaaaag aaagtataat gatggaaagc cgatcttgtg gtttctagaa aactgtgagc
     1441 tatcgtcagt tgatttaaga agatcacttg ttcagatgaa actggcaggc tggtttgatg
     1501 gcagctcggg gatcatgttc ggtagaagtg cggcagagga tttgttagat tataaaatga
     1561 aggatgtata cgaagatctg tcaaaggaat taaaactacc gatcttgtat gatattgatt
     1621 gtggtcatgt accgccacaa ctgacattga ttaatggtgc ctatgcagaa gtagcggtag
     1681 aagatagaaa agcgactgtt ttgcaaacgt ttatttaaaa aattttttcc tgccttggag
     1741 tctatcaaag accaataaat ctaaatagaa aagaaagtga aagaagctga caaaatgctg
     1801 tcagcttctt tttgtataac atattagaaa gtctaattat ctataactgt cacatcgatt
     1861 aactgtttca cgtcattggg atttcctgta ggaatagcta ctacagatgc attatcaatg
     1921 atttcttgag ttgtttctct aatgaacgct ggataagatg tttgatctgc aggcatttgc
     1981 caatgtgaag gaactggata actagtgatt gtcccgtcat ggttacttga atacactaaa
     2041 actccctggt aactgtattt cccagtgagc ataaccacat ctttcggata gactgcactc
     2101 cctgcatcat aaggggcgat aggtgttcct gcaggataat gaataacatg taattcgaaa
     2161 tcacccgatg tatctctaaa agtattacct ctaatagcta gccaaacacg tgcgtactca
     2221 atttgtagat cagaatatcc tgctaacgga tctattgact gagaagactg tgaggaggtt
     2281 gtgtctattg ttgaagaaga aatatttgtc tcaactgtac tactagatgt agaagaagta
     2341 ttgggctctg agactttact gcttgttgaa gatgtattcg tttcagtaga agctgatttc
     2401 tgagtacttg atttttcagc tgaactggtt tgtctgattg aacttttgtc tgtggtcgca
     2461 gatggatcac tattttcttt actaggagac gtacatccta caaggaataa agagaccaac
     2521 aataaaataa aacctaactt tttcatataa aatacctcat tattttttta ataccagtat
     2581 agcataatcc tgaatttttg aatccgtaaa accttgtcta tgaatataaa tagagtgtat
     2641 cttctttgat aaatgtcaga aaaagtgtga caaataatca tcagtaaaat tacttgtcac
     2701 actattgata aggagtattt aattgaagag aatgattagg agaaaggatc tccgtacttt
     2761 ttatagtaat catagaatcc aagtttgtat agacgaatcg ttttttctct tatttcttct
     2821 gtttcaaaat ggactgcttg aagaatcaag tctgtgaatt ctataggagc tgttccattc
     2881 gcagtaatca aattacgatc tttcacagct tgttgttcta taaaatcttc tttattctga
     2941 taattttccg attctaacca aggaaattgc gcatttccgg tgtgtttgta accttgcaat
     3001 aatccgctct gagctaaata atcaactgcc ccacaaatag cagctactgg tacgtctgtt
     3061 tttaacaatt tatcgattat ttgatataaa tgatgatttt ttatgtccca tgaattgcca
     3121 ccgatcataa taaaaaggtc aactttttca ggtatttgtt cgattaagta atcgattttt
     3181 gtttttaatc ctccaatgga agtgacctct gattggatag aagcagtttt gttttgccat
     3241 ttctcatttt ggtttagtgt gctagtaata taagcacctt cccaatcggc atattcctct
     3301 aatagaaacg taatcgcagt tttcattttc aagctctcct ttactatcta taacaaggac
     3361 aacagtttta acctacagac attcattaaa attgggtagc gacagcgaaa acaacaattc
     3421 ttaagtttta gacagagtat acagatagtc aacttaatta aatacgaact aaataaaaat
     3481 gacaggtaat catttttgtt taccagcatt ataacatttt gcatacatgg atgacaaaag
     3541 aaccaattcc cacttgcttg agtacaatat taaaagaaac tggaaggcaa aaagtaatta
     3601 tagaaacgaa aaataaatca tatcgggcac tcagacatga aaacaaccct agagatatat
     3661 aactaggtaa ccagtttaat gaaagaaaaa atcatccaag aagtcgattc ttggattttt
     3721 tagtggattt gttcgccaaa tacgtttagt atgcgtatat gagaggttgt aagcaaagcg
     3781 ttgtggtctg agcacttaag gaggaatctg tgaaaatagc ttttcatatt tttgcagatt
     3841 ttgacttaat gccgaaggtc agctttgcga ttaccgttta tcgagaggct gtaaaagcac
     3901 cgtgtagctc cgatgacaat aagcaagcat ttttagagct aggtttcctt gattctaaat
     3961 atgcggctta tttcaatgag ttggtgcttg aacgcgattt taagaaggtc gaaaacaggc
     4021 acgacttttc gacgtaagga gaaagaattt caatgataga aacaataaat cccgcagaaa
     4081 acaaggtttc tgcgtacatt catatcccat tttgcgagca tatctgctat tactgtgatt
     4141 ttaataaagt ttttttagaa gggcagccag tagatgagta tgtggaaatg ctgctgaatg
     4201 aaatgaaaat aatgctagaa agtcatccga cagcgaattt agaaacactt tacgtagggg
     4261 gcgggacacc aacttcttta tctgctaaac aacttgatcg actgttatca ggtgctagag
     4321 agatcctgcc ttttcaaaat ggtaatgagt ttactgtaga agcgaatcca ggtgatttga
     4381 cccgtgaaaa acttcaagta atgaaaaact atggcgtcaa tcgtttatcc atgggagtgc
     4441 agacatttga taatcgctta ttgaaaaaaa ttggacgtaa acacaccgca gaagatgtgt
     4501 atcaaactat gcaattttta gaagcagaaa attttaccaa tgtaagcatc gatttgattt
     4561 atgcattacc gggacaaaca ctagaaggat atctggatac tttggaaaaa gctttggctt
     4621 tggatctgcc tcactattct ttgtactcat tgatcctcga aaacaaaaca atgttcatga
     4681 actgggtacg acaaggaaga ctcgaactgc ctgatcagga aacggaaacc agaatgtttg
     4741 aagagacgat tgaagcaatg gaaaagcatg gacgtcacca atatgaaata agtaactttg
     4801 ggctctccgg acatgaatct caacataacc tcatgtactg gaacaatgcc cactattttg
     4861 ggtttggagc tggtgctagc ggttatcttg gagatattcg ttaccgcaac aaaggaccaa
     4921 tccaacacta tttacgccca ttacgagcaa atgaacgccc ggtattggaa gaagaaaaat
     4981 tgacacgaaa aaatcaaata gaagaagaaa tgttcttagg actaagaaaa aaaacaggga
     5041 ttactaaaaa acatttctat gatcgctatc aaaaaacaat tgaatcggtg tacggtgatg
     5101 tgctaaggga attagagcaa gaagggttac ttgttaatga agaagaccgt gtttatttga
     5161 caccaagagg gacctttttg gggaacaatg tgtttgagcg ctttttgttt gaaaaatagt
     5221 ttaaagaata tttgtttttt atctgaattt aaataaaaaa agttcaaaaa tacaataaag
     5281 gactattgaa acgttgatat aactggattt acatcaatta tttagaatta gcactcttgt
     5341 gtttagagtg ctaaaaaatg gagtctttct cttgacagga gaagggcttc ttgctatatt
     5401 tataagtgga ttagcactta gaatatatga gtgctaatga gaggtgagga agatgttaac
     5461 aaagcgacaa aacgacattt tacgtctgat catccaacac tatacgagta gtggcgtgcc
     5521 cgttggttcc aaaacattga tgtctgaggg agtcaaggcc agctcagcaa cgattcgcaa
     5581 tgatatgaaa gcattagaag atgaaggatt gctgttgaag actcactctt cttctgggcg
     5641 gattccatct gctttaggct atcgttatta cgtggatcat ttgttaaaac ctgctcgcgt
     5701 agcagaagat gatttgctgc tgattcgtca gtctctaggc agagaatttc atgaaatcaa
     5761 tgagattatt aagcaatctg ctgagatatt atctgagctg acaagctata cgactttttc
     5821 attgggacct gaaataaaag accgccgact gactggcttt cgtatcgtac ccttgaataa
     5881 tcgacagatt attgctatcg tcgtaacaga tatggggaat gtagaaagcc aagtgttcac
     5941 tcttcccgaa tatatgggaa gtcaggaact agaaaaaatg gtacagattg taaacgaccg
     6001 tctagtcgga gatcctctga tgacggtgta ccataaactt cgtacggaga ttccgatgat
     6061 tcttcataaa tatttccaaa cgacagaagg gatgtctcag ttatttgaca ccgttcttgg
     6121 tcatgttttt gaagataagg tttatgtcag tggacaaatg aatctcttgg attatgagcc
     6181 ccgtcaagat cttgatcagt tcaaatcgat gttttctttt atgaaagact cagacggact
     6241 tacacaaatg atcactccga tggatagcaa tattcatatc aagattggtg ctgaattagg
     6301 caatgattta ttgcagaata tgagtatgat tcaagcaagt tacgagatag atggtcatgg
     6361 aagaggtaca atagccttgt tagggcctac tagtatgcct tattctcgga tgttcggtct
     6421 agtcgatgtt tatcgaagag aactagcagc taaacttgct gattactacc gttcgcttga
     6481 cttgtcaaac tcgtaatgtg aataacgaag ctatttgcag ctgatcgttc tctctttctt
     6541 agtaagagca atcaaacagc tgactgaata atgaaaggaa gactgtatcg tgaaagaaaa
     6601 tcaagaagaa ctagataaag aaatgacaga tgctcagcca gaaccggaaa tcgatgtcga
     6661 agctgcagaa gattcgggta tttcagaagc tgaagcggaa gaattcgaaa cagcgaaatt
     6721 aaaagctgag ttagaagaaa tggaagataa atacttgcgg gctcgtgctg aaatcgcaaa
     6781 tatggcaaat cgaggaaaaa atgagcgtga gcaattacaa aaataccgtt cacaagattt
     6841 agccaaaaaa ttacttccat caattgacaa cctggaacgt gcattagcca cagaagtgtc
     6901 agatgatcaa ggggcaggtt tgaaaaaagg cgtggaaatg gtcttggaaa gtttgcgtaa
     6961 cgcactggaa gaagaaggaa ttgaaaaaat ccctgctaaa ggagaagcct ttgatcctaa
     7021 tcttcatcaa gcagtacaaa ctgttccagc gactgaagat acaccagccg atacgattgt
     7081 agaagtacta caagaaggct acaaattaca tgatcgcgtg ttaagaccaa ccatggtcat
     7141 tgttgcacaa taattgtgcg gatataaaac ttacactttc aattaaagaa ggagacgtta
     7201 attatgagta aaattatcgg tattgactta ggtacgacta actctgcagt agcagtattg
     7261 gaaggaaacg aagcaaaaat catcgctaac ccagaaggaa accgtacaac accttctgtt
     7321 gtatcattca aaaatggaga aatccaagta ggggaagttg caaaacgtca agctgtgaca
     7381 aacccaaata cgatttcatc aatcaaacgc catatcggtg aagcaggtta caaagtagat
     7441 gtagaaggta aatcatatac accacaagaa atctcagcaa tgatccttca atatatcaaa
     7501 ggattcgcag aagactattt aggtgaaaaa gtcgaaaaag cagttatcac tgtgccagct
     7561 tactttaatg atgcacaacg tcaagcaaca aaagatgctg gtaagatcgc tggtctagaa
     7621 gtagaacgta tcgtcaatga accaactgca gctgctcttg cctatggttt agataaaaca
     7681 gatcgcgacg aaaaaatctt agtattcgac cttggtggcg gtacttttga cgtatctatc
     7741 cttgaactag gagacggtgt ctttgatgta ttgtcaacag ctggcgacaa ccatctaggt
     7801 ggggacgact ttgataacaa aatcatcgac tacatggtag cagaattcaa aaaagaaaac
     7861 agcattgact tgtctcaaga caaaatggct ttacaacgct tgaaagacgc tgctgaaaaa
     7921 gcgaaaaaag atttatctgg cgtatcaagc acacaaatca gcttgccatt tatcacagca
     7981 ggagaatctg gaccattgca tttggaaatg acattgacac gtgcaaaatt tgatgaatta
     8041 acagctgact tagtagaacg tacaaaagta ccagtacgtc aagcgctaaa agatgcaggt
     8101 ttatcacaat cagaaatcga cgaagttatt ttagtcggtg gatctactcg tatccctgca
     8161 gttgtagaag cagtacgtaa agaaacagga aaagaaccaa acaaatcagt taaccctgat
     8221 gaagtagttg ctatgggtgc tgctatccaa ggtggcgtga tcactggtga tgtgaaagat
     8281 gttgttttac ttgacgttac accattgtca ttaggtatcg aaacaatggg tggagtgttc
     8341 actaaattga ttgaccgcaa tacaacgatt ccaacaagta aatcacaagt attctcaact
     8401 gcggcagata atcaaccagc agtagacatc catgtattac aaggtgaacg cccaatggct
     8461 gctgacaaca aaactctagg aagattccaa ttgacagata ttccagcagc tcctcgtggt
     8521 attccacaaa tcgaagtaac attcgatatt gataaaaacg ggattgtaaa tgtatctgcg
     8581 aaagatctag gtacacaaaa agaacaaaaa atcacgatca aatcttcttc aggtctaaca
     8641 gacgaagaaa tcgaacgtat ggtaaaagat gcagaagcaa atgcagaagc agataaagct
     8701 cgtaaagagg aagttgacct acgtaatgat gtagatgcat tgctattctc tgtggacaaa
     8761 acattgaaag aattagaagg caaagtggac gaagaagaag tgaaaaaagc agaaacagct
     8821 cgcgatgaat tgaaagccgc tgttgaagca aacaacatcg aagaaatgaa aactaaacgc
     8881 gatgcattga acgaaatcgt tcaaaacttg actgttaaac tatacgaaca agcggcacaa
     8941 caacaagctc aagaaaaccc agaagcagct caaggcggag cagatgatgt cgttgatgct
     9001 gattttgaag aagtcgatgg cgacgataaa taatcagttc actgaatttc gagagaaggc
     9061 caaagttgct actttggctt tttcttttga aaaatagttt gaagtatcgg tttttgtttc
     9121 aaacaaaata tgatatgata acaagcgacg tgaaaatgat gagaaccaat aggaggaaag
     9181 cctatggcaa caaaacgtga ttattatgaa gtcttagggc tctcaaaagg agcatcagaa
     9241 gatgaaataa agaaagctta tcgtaagctt tcgaaaaaat accatcctga catcaataaa
     9301 gaacctgatg cggaagaaaa attcaaagaa gtatcagaag catatgagat tttaagtgac
     9361 ccgcaaaaac gcgctgcata tgaccaatac gggcatgcag gcacagatcc aaattacggt
     9421 gctggtggag ctggtggcgg atttggtgga ttcggcggct tctctggtgg cggcttcggt
     9481 ggttttgaag atatcttcga ctctttcttt ggaggaggcg gtcggtcagt cgatccaaat
     9541 gcgcctcgtc aaggtgctga tttgcaatat acggtcgact tgtcttttga agaagcgatt
     9601 tttggtgtag aaaaagaaat caagtataat cgggaagaaa tctgtcatac ttgtggtggg
     9661 aacggtgcaa aaccaggcac acaaccaact acttgtcata aatgtcatgg tgcaggtacg
     9721 attaatgtag aacgccaaac accacttgga cgagtgatga gccgtcaaac ttgtgatgtt
     9781 tgtcatggga cagggaaaga aatcaaagaa ccttgtccaa cctgtcatgg ctcaggacat
     9841 gagaagaaag cacattcagt taaagtgaat gtaccagctg gtgtagaaga aggacaacaa
     9901 atgcgtttag ctggtcaagg agaagctgga gaaaacggcg gaccatttgg tgatttgtat
     9961 gtggtcttcc gtgtagaaga aagtgatatc tttgatcgtg aaggttctga aatttactat
    10021 gaattgcctt taagttttgt acaagcagct ttgggggatg aagtccaagt accaactgtc
    10081 catggagatg tgaaattgaa gatccctgct ggaacacaga caggtacgaa cttccgttta
    10141 cgtggaaaag gcgctccgaa gttacgtggt ggtgcaacag gcgaccaaca tgtcaaagta
    10201 aaactgatca caccgaaaaa tctaaatgaa caacaaagag aagcattacg tgcattcgct
    10261 gaagctggcg gcatcaaagt tgaagaacag caagaagacg gtttcttcga taagtttaaa
    10321 gatgcttttg gcgggaaaaa gcggaagtag tttttctagt aaaaacgcta agtcattaat
    10381 aattaaaaac cagacgaaca atcatatgtt cgcctggttt ttttgtagtt gtcatgtttg
    10441 taccaacaaa aaagccagct ttagaatatg ataatcgtca tagtttctag gggtataacc
    10501 agtgagagaa tggaacttgt tcaatcgata ttgcaaggtg tttttatgaa taaataattc
    10561 ttctgagcaa tttttgatgc ttccgttatt tttactgaag catgccaaag tcttcttgaa
    10621 caaatgtact tcttcttctg tcaggctttt caaaatacgt tttacatata atgttcgttg
    10681 atctgacggg attgctggta aaacgatccc gaggtctaaa tcttcaaaaa agcggatctc
    10741 gttcacagat tgatggaaag ctatccattc gcaaacttgt ttggcatgaa ggaaagattg
    10801 cggtgtctct tgctctgtat aacaaagcga cccaattcca aagtttagct gtaactgata
    10861 cattttatta aactttgtct ggattttatc aagcaatgat agcaatgagg ctctggaaat
    10921 ccgattgact aggataatga tttgttgtct atgtacagtg agcaaagcct gtccgtaata
    10981 agagaagacc ctttcgagca actccaataa aggattgatc cgttcttgac acaattgctg
    11041 attgtcagaa agttgtccca caactacata atatggagaa gaaaaatcaa gctcaattaa
    11101 gtgagggtgg tatcgactat gtacttgata tcatcaatga accgatggta ctttcttatg
    11161 cgatggaaca ggtagagcga ttgcttgttt gtgcaggaaa acaagcgatg cggctgatac
    11221 gattaggtga gaaaataaga agataaaaat acacggtccc ttcatggcga ccgtgcattt
    11281 ttggttttta ttaaatattt aatactttat tcaagaaatc ttttgtccgt tcattttgcg
    11341 gattttcaaa gatttgttga ggcgttccat cttctaaaaa gtttccgtcg tcaatgaaca
    11401 tgacgcgatt ggccacttct ttagcgaacc ccatttcgtg cgtaacgata accatcgtca
    11461 taccttgttc tgctaacttt ttcatgacgt tcaagacatc accgaccatt tcaggatcga
    11521 gtgcagaagt aggctcatca aaaagcatga tgtcagggtt catcgctaga gcacgagcaa
    11581 tagccacacg ctgtttttgt ccaccagaaa gcatttcagg atacatatct tttttatctt
    11641 ctagaccaac tgtctgcagt aatttgattg cttttttctc tgcttcttct ctagattctt
    11701 tcttcaggtc catcggtgct aaaatgatgt tttccaaaat agaaagatga gggaataggt
    11761 taaagtgttg gaataccatt ccgatatgtt gtctcaccaa gttgatatct gtgcttttat
    11821 ccatcaagtg ggcgccgtca ataataattt caccgtcaga aggttcttct aaacgattca
    11881 agcaacgcaa gaatgtactt tttcctgaac ctgaagggcc aatgatacag acgacatctc
    11941 cttcgttgat ggatacattg atgtcgttta atactgtgtt atcgccgtat tttttgacta
    12001 aatgctcaac taagattttt tctgccatct tacttcacct tcttttctaa aacttttgct
    12061 aattttgtca atgctgtgat caaaatcaag tacatgatcg caatgatcaa gtaaacatac
    12121 gtactttgtg ttgtacgtgc aacaatgatc ttaccagttt gcagcaattc gactacaccg
    12181 attgcggaaa tgatcgttgt gtcttttaat gagataacga actgattgac gaatgatggg
    12241 atcatgatgc gtatcgcttg cggcaaaatg attttttgca ttgtccgatt ataagaaaga
    12301 cctaaacttc tggaagcttc catttgtccg acaggaacgg cgttgatccc gcctcgaaca
    12361 atctctgcga tataagcact tgcgttaaga gtcaaggtga taacaccagc aaggaagtca
    12421 ggaatcgtaa atccaacgat accaggcaac ccaaagaaaa taaagaacgc aagaaccatc
    12481 attggaattc ctcggatgat atcgacataa atactagcaa ttgtgcgaag agcgcgtatt
    12541 ggtgctacac tgaataatcc aaaaatcaca ccgattacta tagccaaagc aaaggagatc
    12601 aaagttagga gaattgttct ccaaagtcct ttcaacaatg cttgatagtt gttttggata
    12661 agtccaagaa atgttgattc atcagcagtg gaagactcac tggaagtttc tgaaccatcc
    12721 gctacatatt ctttaataat cttatcgtat tcgcctgttc tttttaattc tttcaatcct
    12781 tcattgaaca tattaagaag ctcaggattt tgtccttttt taacggcaaa tgcatatttt
    12841 ccgccagatt cttttttgat cggtgtttcc aattcttgtc cttgtttgat cgcataaccg
    12901 attaccggat aatcatccat cattgcatcg attgcgccaa cttttaaccc gtcgtacaat
    12961 tgatcagcag catcaaaata tttgatagaa tagccatatt cagattcatg gtcgcttaaa
    13021 aattcagcac tttcggtacc aacttttgct ccgactgttt ttcctttcaa atcttcataa
    13081 gacttgatcg aagtatctcc ttttttgaca gctaattgga tcccgctatc aaagtaagaa
    13141 tcagaaaaat catagctttt ctttcgttca tctgttacag tcattccggc aatcattccg
    13201 tctgcttgtc ctgattccaa agcttgcaaa gcagcagaga aaccacggta gtccattcgg
    13261 atgttaaatc cttgcagttt ggcgatgttt tccatcaatt ttacatcgat gccttcgtac
    13321 tcgccctttg cgttttgaaa ttcaaatgga gcaaaagcag agtcactggc aattacgtat
    13381 acgtcttttt tgggctctat tttttgcatt gtctcgcttg tgcttgactc tgccgttgta
    13441 gtgttgtcag cttgtgcaac atgaccaaac gcaagtgtca caagtcctgt cattaagaaa
    13501 acaaatgagt gaaataaatg ctttcttttc ataatcaacc tcctaaattt ccaatatttt
    13561 attattatag gcgaaaagtg ctgaatcaga caagactgta agctcgaaaa aatgaaaaaa
    13621 atgcgagttt ctaacatttt atgttataaa acataacatg tataataaga taaaaaagtg
    13681 gcgaaagtat gttcggtgtg gtataatttt ttagtaagga atgcctttgc tttccttttc
    13741 tttttattgg tcgtaaaatg aaaaggaatg aatgaaaagg aggacgaaga atgattgaga
    13801 gagatacctc ccgacacttt gagcttgtct cgaaatacca accggctggt gatcagcctg
    13861 aagcgattaa ccaattagtg gatggcgtag tcggcgggaa aaaagcacaa atcttattag
    13921 gtgcgactgg aacagggaaa acatatacga tttccaatct tattgagaaa gtgaataaac
    13981 caacactgat tattgcccat aacaaaacat tggctggtca gctgtacggg gaattcaagg
    14041 aatttttccc taacaatgct gttgaatatt ttgtttctta ttatgattat tatcagcctg
    14101 aagcttatgt gccttcaagc gatacgtata tcgaaaaaga ttcaagtgta aatgatgaga
    14161 tcgacaaatt acgacattcg gctactagtt cattattaga aagaaatgat gtgatcgtca
    14221 ttgcatccgt ttcatgtatt tttggattag gctcgccttt tgagtaccaa aaacaagtag
    14281 tttcgattcg acagggagca gaactagatc gcaatcagtt gattcgtgac ttagtcagta
    14341 tccaattcga aagaaatgat atagattttc agcgcggtcg ttttcgagtg cgcggtgatg
    14401 tcgtggagat ttttccggct tcaagagatg aacgtgcctt acgtgtggag ttttttggag
    14461 atgaggtcga acgtatccga gaagtcaatg cactgacagg tgaagtttta ggagaaacgg
    14521 aacacgtagc aatcttcccg gcgactcact ttgtgacgaa tgatgaacat atggagcatg
    14581 ccgtagcgaa tatcaaagcg gaattagaac agcgtttaac tgtcttgcga aatgaaaaca
    14641 aacttctgga agctcaacgt ttagaacagc gaaccaatta cgatattgaa atgatgttag
    14701 aaatgggcta cacgtcggga attgaaaatt attctcggca tatggacgga cgtaaagaag
    14761 gtgaaccgcc ttatacgcta ttggatttct ttcctgaaga ttttttgatc gtggcggacg
    14821 aatcgcacgt aaccatgcct caaatcagag gaatgtataa tggtgaccga gcgagaaaac
    14881 agatgctagt agattacggt ttccgtttgc catctgcttt agacaatcgg ccacttcggt
    14941 tagaagagtt tgaaaaacat gtcaatcaga tcatttatgt ctctgcgacg ccaggaccat
    15001 atgaacatga acaaacagat accgttatcc aacaaatcat ccgtccgaca gggctgcttg
    15061 acccagtgat cgaagttcgt ccaatcatgg gacaaatcga tgatctagta ggcgaaataa
    15121 atgaacgagt cgaaaaagac cagcgggtgt ttgtaacgac attgacgaaa aaaatggcag
    15181 aagacctcac agattatttc aaagaattag ggatcaaagt caaatatttg catagtgata
    15241 tcaaaacgct ggaacgaaca gaaatcattc gagatcttcg attaggtgaa tttgatgtgt
    15301 tagttggaat caatttgctt cgtgaaggat tagacgtacc tgaagtttcc cttgttgcta
    15361 tcttggatgc ggataaagaa ggctttttac gtagtgaacg ttccttagtc caaacgattg
    15421 gacgagctgc tcggaatgaa gaaggtaaag taatcatgta cgcagacaaa gtaacagatt
    15481 ctatgcgtct agcgatggat gaaacgtcaa gacgtcgtac gattcagcag aagtacaatg
    15541 aagaacatgg gatcgttcca aaaacgatca ttaaagaaat ccgagatttg atttctatta
    15601 cgaaagaatc ggaagatgat acaaaagaag cggtacaagt ctcgtacgaa gaaatgacga
    15661 aggaagaaaa agacacgtta ttgatgaaat tagaaaaaga aatgaaagat gcggcaaaag
    15721 ctttggattt tgaaacagcc gcgaatgtgc gagatatgat tttagaatta aaagcttcaa
    15781 agtaggagga caacatggct aacgataaaa ttacgattca tggtgcacgt gcccataact
    15841 tgaaaaatat agatgtaacg attccacgag ataaattcgt ggtcgttaca ggtttgtccg
    15901 gttctggaaa aagttcccta gcatttgata cgctgtacgc tgaagggcag cggagatacg
    15961 tagagagcct ttctgcttat gctcgtcagt ttttaggaca aatggataag ccggacgtag
    16021 atagcatcga cggattgagt ccggcgattt ctattgatca gaaaacaaca agtaaaaatc
    16081 ctcggtcgac agtaggtact gtgacggaaa tcaatgacta tttacgcttg ttgtttgcac
    16141 gtgtaggaca tccgatttgt cccaacgatc atatcgagat taccagtcaa tcagtagatc
    16201 aaatggcaga tcaagtaatg gctttgcctg agcgcacaaa gatccaagtg ttggcaccga
    16261 tcgtggtaaa gaaaaaagga caacataaaa aaatattcga acgaatccaa aaagagggat
    16321 acgtccgcgt tcgagtcgat ggtgagatct atgatttaag tgaagtgcct gagctggaga
    16381 aaaacaaaaa acatgatatc gcgatcgtca tcgaccggat cattgtgaaa gaagggattc
    16441 gttctcgcct atttgattct ttagaggcgg ccctgcgatt agcagacggc tatgctctgg
    16501 ttgacgtgat cggtgaagaa gaaatgttgt ttagcgaaca ctatgcctgt ccttattgcg
    16561 gatttactgt tggcgaacta gaaccaagac tgttctcatt caatgcgcct tttggagctt
    16621 gtccagactg tgatggttta ggtgtcaaat tagaagtcga tcaagattta gtgattccag
    16681 atagaacgaa gacacttagt caaggtgcta tcgttccatg gaatccaatc agctctcagt
    16741 attatcctca aatgttagcg caggcttgtg aaagtttcgg tatcgattta gatacaccat
    16801 tcgaagaatt accggaagag catcaggaga tcatcttgaa tgggacagat gatcttttcc
    16861 atttccatta tgaaaatgat tttggtggcg ttcgcgatgt agaaacgact ttcgaagggg
    16921 tattgaagaa tatcgagcgt cgttatcatg atacgaacag tgatttcacg cgtgagcaaa
    16981 tgcgtcttta tatgacagaa ttaacttgtc agacatgtaa aggttatcga ttgaatcccc
    17041 aagctctatc tgttcaaatc aatggaacga atatcgggca aacaaatgag ttgtctattc
    17101 aaaaagcagt agatttcttc tcaaacttaa cgttatctga acaagagaaa gtaattgcca
    17161 aaccgatttt aaaagaagta aatgacagac tttcattctt ggaaaatgtg ggactagact
    17221 atctaacttt gagtcgcgct tccggaacat tatctggggg agaagcgcag cgaatcagat
    17281 tggctacaca aattggatcg aatctttctg gagttttgta tattttagat gaaccatcga
    17341 tcggattgca tcaacgcgac aacgatcgac tgattggctc attaaagaaa atgcgtgatc
    17401 ttggcaacac cctcgttgta gtcgaacatg atgaagatac gatgtgggca gcagattatt
    17461 tgattgacgt cggacctggg gccggagaac aaggcggaca aatcgtggct gcaggcactc
    17521 ctgaagaagt agctaatgat gaaaattctt tgacagggaa atatctttca gggaaaaaat
    17581 caatccctgt accaaaagaa cgccgtaaag gtaatgggaa agcaatcaag atcactggtg
    17641 cttcagaaaa caatctgaaa aatattagtg ttgaatttcc attaggagaa tttgtagcag
    17701 tcacaggtgt gtctggttct ggaaagagta cattggtcaa tagtatctta aagaaatcgt
    17761 tagcgcaaaa attaaataag aattctgcta agccaggtaa attcaagaca atttccggct
    17821 acgaaagtat cgaaaagatc atcgatatcg atcaaagccc aatcggccgg acgccgagaa
    17881 gtaatccagc gacttataca agtgtatttg atgatatccg tgggttattt gctcaaacga
    17941 acgaggcaaa aatgcggggt tataagaaag ggcgttttag tttcaacgta aaaggcggtc
    18001 gttgtgaagc ttgtcgcggg gatggaatta ttaagatcga aatgcacttt ttgcctgatg
    18061 tctatgttcc ttgtgaagta tgtcatggta aacgatataa ttctgaaaca ttagaagtgc
    18121 attacaaagg aaaaagcatt gctgatattt tggaaatgac agtagaggat gctgtagaat
    18181 tcttcaagca cattccaaag attcatcgca aactgcaaac gattgttgat gttggcttag
    18241 gttatgtgac gatggggcaa ccagcaacga cattgtccgg tggtgaggca caacggatga
    18301 aacttgccag tgaattgcac aaaatctcta atggaaagaa tttctatata ctagatgaac
    18361 caacgacagg acttcatagc gatgacatcg cccgcttgtt gcatgtatta caaagattag
    18421 tagatgctgg taacacagtt ttagtgattg aacacaatct agatgtaatc aaaacagcag
    18481 attatatcat tgatttagga ccagaaggtg gagaaggtgg aggaacgatc cttgcgactg
    18541 gaacaccaga agaaatcatt aacgtaaaag aaagttatac aggtcactat ttgaaaaaaa
    18601 taatggtata aagaggatag atagctgaga tagttaaaaa aacataaaaa aatgaatttt
    18661 tgcttgtttt attaatatca gtgttctaga atcattacat ggtagttctt tctacactga
    18721 ttggtataga taaaaacttc ctataagttc aatccgttca aaaaagaatt gagcaatcgg
    18781 aggactttcc tttgattgct tttttcttta tggtatgata ttccttgtgt agttggaaaa
    18841 gaaaaacaag agattatgag taagtgagag ataatgatga acagtattga aaatgaagaa
    18901 tttatcccta tattattagg aagcgacatg aacgtttatg gtatggcacg ttcttttaat
    18961 gaagcatacg gcaaaatctg ccaagcatat gcttcggatc agttagcccc tacccgctac
    19021 agtaaaattg tcaatgtaga agttattcca ggatttgata aagaccctgt atttatcgag
    19081 acgatgcttc gtttggcaaa agaaagatat tctgataaga gtaaaaaata tcttttgatt
    19141 gcttgtggtg atggatatgc agaattgatc tcccaacata aacaagagct gtcagaatat
    19201 tttatctgtc catatattga ttattcttta tttgaaaggc tgatcaataa agtaagcttt
    19261 tatgaagttt gtgaagaata tgatcttccg tatcctaaaa cattgatcgt cagagaagaa
    19321 atgttagtaa atggacattt ggaacaggaa ttgccttttg aattcccagt tgctttgaaa
    19381 cctgcaaaca gtgtggagta tctttctgtc caatttgaag gaagaaaaaa agcttttatt
    19441 ttagagacta gagaagagtt cgatttaatt ttaggccgga tatacgaagc cggttataaa
    19501 agtgaaatga ttgtacaaga ttttatcccg ggtgatgata gtaatatgcg tgtgctaaat
    19561 gcgtatgtag atgaagatca tcaagttcgg atgatgtgcc ttggtcatcc actattagaa
    19621 gatcctacac cagcatcgat tggtaattac gttgtgatca tgccagatta taacgaaaaa
    19681 atctatcaaa ccattaaggc atttttggaa aaaatcgaat atacaggctt tgccaatttc
    19741 gatatgaagt atgatcctag agatggagaa tataaattat ttgaaatcaa tttgagacaa
    19801 ggtcgtagca gtttctttgt gacattgaac ggcttgaatc tggctcgttt tgtcacagaa
    19861 gatcgagtgt tcaacaaacc ctttgttgaa acgacctatg gaacgaatca atcagataaa
    19921 gcaagattat ggatgggtgt gcccaaaaaa atctttttag aatatgctcg ggaaaatgaa
    19981 gacaaaaaac tagctgagca aatgatcaaa gaaaatcgtt acggtactac tgtattttat
    20041 gaaaaagacc gatcaatcaa gcgctggttg ttgatgaaat atatgttcca taactatatt
    20101 ccaagattca aaaagtattt tcatgtgaaa gaaggctagt tcatggagaa ttttttcagt
    20161 attttaggcg gaatgggcac gatggcgaca gaaagttttg ttcgcttgat caaccaccga
    20221 acgaaagcta caaaagatca agaatatttg aattatgtct tattcaatca tgcaacagtt
    20281 cctgatcgta cagcttatat tttagatcga tcagaagaaa atccaatgcc gtttctgctg
    20341 gatgatattg agaaacaaaa tttattgcga ccaaacttca ttgtgttgac ttgtaacacc
    20401 gcacactact tttttgaaga gttacaagcg gcaacggata ttcctatctt gcatatgcca
    20461 agagaagcag caaatgaact tgtccgtcag catacgacag ggagagtagc gattctagga
    20521 acagaaggaa gtatgaaagc tggaatctat gaacgcgaag tcaaaaatct tggtttcgaa
    20581 acagtgattc ctgatactgc gcttcaagaa aaaatcaatt acctgatcta tcatgagatc
    20641 aaggaatcgg attatttgaa tcaagagctc tattacgaaa tcttagagga agcggtagag
    20701 cgcctaaact gtgaaaaagt catcctaggt tgcacagaat tatctttgat gcatgaattt
    20761 gctgaagata atcattaccc agtgatcgat gctcaatcta ttttagcgga tcgaacgatc
    20821 gaacgtgcat tagctgaaag aagcgaggca ttggatacag catcggaaaa gtaaaaaaat
    20881 tggatttggg gcaaatatcc taaaagatat ttggtccaaa tctttttgta aaagaaaaag
    20941 aaaactggta atgatacgcc cgggatgata taattttatt atgcacaatg tgagaggggt
    21001 tcgtaaatat gacagataat ttgcaattag tcatcataac aggtatgagt ggtgcaggga
    21061 aaacagtagc tatccaaagt tttgaagata tgggctattt ttgtatcgat aatatgccgc
    21121 ctcgattgat tccaaaattc tgggaattga tcaaagaatc aggtaaagtc acaaaaatgg
    21181 ctttaggagt ggatttgcgt tctcgttcct tctttgaaga aatccaaaac atgctgatcg
    21241 aaatcgaaaa tacgaacttc atcgacactt ctatcttatt tttagatgct tcagacgaag
    21301 aactagtggc tcgctacaaa gaaacaagac gagcacatcc aatggcgatg gacggtttga
    21361 tcactgaagg gatacgtaaa gaacgggcga tactagacga cttgaaagcc caagcttccg
    21421 tgatcattga tacgacaaca ttgacaccac gacaattacg tgaaaaaatc aatgagtcat
    21481 tcaaatcaaa gggagaccaa ggatttcgga tcgaatttgt atcatttggt tttaaatacg
    21541 ggttgccgat cgattcggat attgtgatgg atgtgcgttt tttgcccaac cctcattata
    21601 tcccggaact aagaccattg accggaaaag atcagccagt ctatgattat gtgatgtcct
    21661 ttgatgaaac gcaatcattc taccataaat ttttggatct tctggaaact gtattgcctg
    21721 gatacattaa agaaggaaaa agcagcttga cagttgcaat cggctgtaca ggcggtcaac
    21781 accgttctgt ggcattgact gaacgtttgg caaatgcttt agggaaaaac tataaagtca
    21841 atgtgacaca tcgggacaag gataaaagga aagagacggt gaaccggtcg tgaaaatgaa
    21901 aacctaccgg ataagaaaac caaagatcgt agtggtcggc ggtggcactg gtctgccagt
    21961 tatcctgaaa agtttacgaa atcaaggagc agatatcaca gcagtagtga ctgttgcaga
    22021 tgatggtgga agcagcggtg caatcagaga atcgatcgct atggctcctc ctggtgactt
    22081 gagaaatgtc ttagtggcgt tatcggatat gccacaattc tatgaagata ttttccaata
    22141 ccgtttcaaa aaggaagatc agtttttagc caatcatacg atcggtaatc tgatcattgc
    22201 cgcagtttcc gagatgcggg gaagcactta tgaggcgatt caattattag caaaaatgat
    22261 gcatgtagat ggacatgttt atccttcttc agaaactcct ttgaccttgc atgctgtttt
    22321 taaagacgga acgactgcag taggagaatc taaaatcgcg atcaaccgta aaaccattga
    22381 ccgtgtgtat gtctgcaaca caaatgataa aagtgaagcc aaagcggctc gaaaagtcgt
    22441 ttcagcaatt atggacgccg acatggtcgt tttgggaccc ggaagtttat tcaccagtat
    22501 ccttccaaat ttagtgatac cagaaatcgg cgaagcaatg ctcaagacaa ctgctgaaac
    22561 cgtctatatc tgtaatatca tgacccaaaa aggggaaacg gaacatttca cagatgccga
    22621 tcatatacgt gttttacaca agcatttgaa tcagcctttt atcgatacag tattggtcaa
    22681 tacggaaaaa gtgcctgaag attatatgga cccagagatc tatgatgagt atctagtaca
    22741 agtcgcacac gacttcaacg gattgagaaa tgaaggatgt cgagtgattt ccaccgactt
    22801 tttagaattg aaagatggtg gtgtcttcca cgatggagac aaagtagtag aagaattgtt
    22861 ccgtttagtt ttcggttcaa aatattaagc aatttattgt tggagaaagg aggagaatag
    22921 gatgtcattt gctgcagatg tgaagaaaga actaactggt ttggaagttc atcgagaaca
    22981 tgcgaaagcg gaattagctg ctttgctgcg aatgaacggc tctttaggta ttgtgaacca
    23041 gcagtttatt ttgaacgtcc aaacagaaaa tgctgcaatt gccagacgta tctattctct
    23101 tttgaaagat cattatcagg tacgaagtga gctactcgtt cgtaaaaaaa tgaaattaaa
    23161 aaagaacaat gtctatatcg tccggttgaa gcaaggcaca aaagatgtgt tgatggatct
    23221 tgatatcatg gatggtatga tgttcaatgg acacgtttcg gacgagatta tgggaaattc
    23281 gcaaaaaatg cgttcttact tacgtggtgc ttttatggcc tcaggttcag tcaataatcc
    23341 ggaaacgagt cgttatcatt tggaaatatt ttctatttat gaacagcata accaagacat
    23401 ttgcgatatg ttaaattatt acggtctaca tgctcgaacg cttgaacggc gcaatggcta
    23461 tatttcgtat ttaaaaggag cagaaaaaat tgctgatttc ttaacattga ttggtgcaac
    23521 caactcaatg ctccgatttg aagatgttcg aatcgtacgg gatatgagaa attcggtcaa
    23581 tcgtctagtc aattgtgaga cagcaaatct gaacaagacg attgatgcgg cttctaaaca
    23641 aatcgaaaac atcgaattga tcgaagcaaa ggtcggtcta catgcccttc ctgaaaaact
    23701 tcaggaaatc gcagaacttc ggcttcaaca tccagaggtc agcttgaaag aattagggga
    23761 aatgattccc tcaggtgcaa tatcaaaatc gggaattaat caccgaattc gcaaaataaa
    23821 tgaatttgcg gaaaaactgc gtgaagaagc agtttaatcc aaagaaaaaa ctgatacatg
    23881 aagcaggaga tgcgaatgta tcagtttttt ttaaaagcta tacttgcttt tcttccgtca
    23941 aactataatt ccaactttac tggagtaaca taatgttgaa aggcttctat tgttttttaa
    24001 tacaatcgta taaaaagcgg ggagacaact gaatgaagat tttagtttta tacataaact
    24061 tttgcttaat atagaagtcc tgatatagat aggatcaagt atttatctat aactggagat
    24121 taattaaaaa ctggaaggaa ctgctcgatc aaccttgaga actgtttttc ttacttctgg
    24181 tcaagattct cgtggttggt ggataaggat ggttgataag ttcattggat tagctagtcg
    24241 aaaacaacca ggtgtaatta atgtagaagt tttaacaagg ggatggagtg atttattcag
    24301 tgataatcat aagttcataa atagtaattg ggatgtatgg ttggtatacg ttatacctga
    24361 acttgaacta tcaagatttg gtttagaatt tgagaatcga tgtttataaa gggagggcaa
    24421 aaaatgtgct tttttacaac ttttactatc attatgattg cagtactttt tatttttcta
    24481 gatatagcaa agcgaaatac agcattttta ttatatcggg ttttacttcg agcaggtctc
    24541 ataacattta tatcgatcgt tggatttttt ctattcacag ttattgtatt tatttggaga
    24601 actacagcac caccattgcc agagattaca tatggagaat ttccgtttag tcttgaatat
    24661 gaaatagatg aggaaagaat tttgattgaa gatacggtga ttgcagaatt tataggaagt
    24721 tttagaggga atgtgacaac tcaacctaga cgaagttgga gtataaaact tttaaataat
    24781 agtggagagt tgaa
//
LOCUS       DANKKG010000029        24264 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000029 DANKKG010000000
VERSION     DANKKG010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.029"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            137..994
                     /locus_tag="PW004_000996"
     CDS             137..994
                     /locus_tag="PW004_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HDL2725984.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            1109..2029
                     /gene="pstC"
                     /locus_tag="PW004_000997"
     CDS             1109..2029
                     /gene="pstC"
                     /locus_tag="PW004_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HDL2725985.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            2029..2913
                     /gene="pstA"
                     /locus_tag="PW004_000998"
     CDS             2029..2913
                     /gene="pstA"
                     /locus_tag="PW004_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HDL2725986.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            2928..3734
                     /gene="pstB"
                     /locus_tag="PW004_000999"
     CDS             2928..3734
                     /gene="pstB"
                     /locus_tag="PW004_000999"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HDL2725987.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            3750..4508
                     /locus_tag="PW004_001000"
     CDS             3750..4508
                     /locus_tag="PW004_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HDL2725988.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGINLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            4521..5198
                     /gene="phoU"
                     /locus_tag="PW004_001001"
     CDS             4521..5198
                     /gene="phoU"
                     /locus_tag="PW004_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HDL2725989.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            5436..6467
                     /locus_tag="PW004_001002"
     CDS             5436..6467
                     /locus_tag="PW004_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HDL2725990.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGRLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSISIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            6464..7231
                     /locus_tag="PW004_001003"
     CDS             6464..7231
                     /locus_tag="PW004_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HDL2725991.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            7331..8899
                     /gene="liaX"
                     /locus_tag="PW004_001004"
     CDS             7331..8899
                     /gene="liaX"
                     /locus_tag="PW004_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HDL2725992.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEITQVQL
                     STTTGSIYLKDTDK"
     gene            9196..9516
                     /locus_tag="PW004_001005"
     CDS             9196..9516
                     /locus_tag="PW004_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HDL2725993.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            9516..9866
                     /locus_tag="PW004_001006"
     CDS             9516..9866
                     /locus_tag="PW004_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDL2725994.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            10033..10971
                     /locus_tag="PW004_001007"
     CDS             10033..10971
                     /locus_tag="PW004_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HDL2725995.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            10986..11816
                     /locus_tag="PW004_001008"
     CDS             10986..11816
                     /locus_tag="PW004_001008"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDL2725996.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            11860..12885
                     /locus_tag="PW004_001009"
     CDS             11860..12885
                     /locus_tag="PW004_001009"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDL2725997.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            12900..13838
                     /gene="galU"
                     /locus_tag="PW004_001010"
     CDS             12900..13838
                     /gene="galU"
                     /locus_tag="PW004_001010"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDL2725998.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKKLEAEGSKIDHNPKGITEIKEEKKK"
     gene            13963..14403
                     /locus_tag="PW004_001011"
     CDS             13963..14403
                     /locus_tag="PW004_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HDL2725999.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            14406..15008
                     /locus_tag="PW004_001012"
     CDS             14406..15008
                     /locus_tag="PW004_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDL2726000.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDTKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(15085..16191)
                     /locus_tag="PW004_001013"
     CDS             complement(15085..16191)
                     /locus_tag="PW004_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDL2726001.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLASIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            16401..17402
                     /gene="ccpA"
                     /locus_tag="PW004_001014"
     CDS             16401..17402
                     /gene="ccpA"
                     /locus_tag="PW004_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HDL2726002.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(17738..20083)
                     /locus_tag="PW004_001015"
     CDS             complement(17738..20083)
                     /locus_tag="PW004_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDL2726003.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            20362..21264
                     /locus_tag="PW004_001016"
     CDS             20362..21264
                     /locus_tag="PW004_001016"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HDL2726004.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQIFDPEVFPELLAEEVIL"
     gene            21477..23252
                     /locus_tag="PW004_001017"
     CDS             21477..23252
                     /locus_tag="PW004_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HDL2726005.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSFYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYSSSKKMLPWFLGLAVSFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEAKKLKERPFLLSKQEWLKLSLFLLPLVGFSIVYQWIEYKNETTYSIAAIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALDASPTFHAVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYQKGT
                     IKKKKEFYLMGSGDGKTMNDLPIVFAFLKSGYAYAWRYKDFHQGGNYSFGTQEQVEQA
                     QELLNLPLMDNWIDSSDPRTPDFQLTKTAKVANVMIKIYQVLAIPVLIASIAGILLMI
                     LGTAFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            23509..>24264
                     /locus_tag="PW004_001018"
     CDS             23509..>24264
                     /locus_tag="PW004_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2726006.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEENTLCSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFLNRLTPYALILIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQK"
BASE COUNT         7752 a         4336 c         5089 g         7087 t
ORIGIN      
        1 agcatagaaa cttttaatga gatagttttt tttacaatta tgagaattta ttttgttatt
       61 taatgatttt gattcgtttt catcaagtaa tgaaaatgat aaaataaaca tactttagga
      121 ttaaaggaga tttctaatga aaaaacttaa attattctta ccaataatag gattagccat
      181 tcttttatca ggctgttcaa agtggattga tcgaggagaa tccattacgg ctgttggctc
      241 ttctgcttta cagcctttag tagaaacagt tgctgaaact taccagacag aaaatcctgg
      301 aaaattcatc aatgtacaag ggggcggaag cggaactggt ttgtctcaag tacagtcagg
      361 agcagtcgac atcggaaact ctgatttgtt tgcggaagaa aaatcgggga tcgatgcctc
      421 agcattagtc gatcatcgag tagcagttgt tgggatcact cctattgtga acaaggatat
      481 tggcgtaaaa gacatcagta tggaaaacct gaaaaaacta tttacaggcg agatcaaaaa
      541 ctggagtgaa gtcggtggaa aagatcagcc agtagttata ttgaaccgcg cttcaggaag
      601 cggaacccgt agcacgtttg aaaaatgggt attggacgga gaaacagctg ttcaggcaca
      661 agaacaagat tcaagcggaa tggtgcgcca gatcgttgcc gacacacccg gtgcaatcag
      721 ctatgttgct ttttcttatg tttccgatga tgtagatacg ttgaatgtta acggtgtaga
      781 accgacagat gaaaatgtga caacgaacaa atggacgatt tgggcatatg agcatatgta
      841 cacaagagat catccaaaaa aactgacaaa agaattttta gactatatct tatctgatga
      901 agtacaagat aacatcgttg gagaactagg ttatatccct gtttctcata tgaaagtcga
      961 acgtgattgg caaggaaata tcgttaaaga gtaatcttta cacaaaattt acaaacttag
     1021 ctaaactttt acatgttatt aaaaccaaat tgatgttcaa tttgtacact gagtgagtat
     1081 gttaaacctg attaaggggg agtcatctgt ggaagatgtg aaaaaagtat tattaacaaa
     1141 atcaaaacgt tcaaaaatgg aacagcgtgg aaaattcatc agttttttat gtatcgcgct
     1201 gatcgtgttg gttgtattat ctatttttta ttttgtagct agtaaaggat tggccacctt
     1261 ttttactgat aagatcaatg tatttgattt tctgtttggt acagattgga atccaagcca
     1321 aatcggagca aatgggaaac caatggtagg ggcattgcct atgatcacag gttcgtttat
     1381 tgtgaccttt ttatctgcta tcgttgccac gccatttgcc attggtgcag cagtattcat
     1441 gacggagatc tctcctaaaa acggaacgaa gatcttacag ccggtcatcg agctgcttgt
     1501 aggtattcct tctgtcgttt atggttttat cggtttatca gttatcgtgc cttttgtcag
     1561 aagtattttt ggcgggacag ggtttggtat cttagctggt acatttgtgt tgttcgttat
     1621 gatcttgccg actgtcacaa cgatgactgt tgatgcatta aaggcagtgc cacggcacta
     1681 tcgggaagct tcattagcgc ttggtgccac aagatggcaa acaatctaca aagtcgtgct
     1741 acgagcagcc gtgcccggga ttttaacagc agtcgttttt ggtatggctc gtgcgtttgg
     1801 tgaagcctta gctatccaaa tggtcatcgg taatgcagca ttgatgccaa caggacttac
     1861 tactcctgct tctacattga cgtccatctt aacaatggga atcggaaata caatcatggg
     1921 aacagttgaa aataatgtgt tatggtcatt agcgctgatc ttgctattga tgtcattatt
     1981 ctttaatatc gtgatccgta tgattgggaa gaaaggagag ctgaaataat gaacgcaaaa
     2041 cgctcagata aaattgctac aggtgtttta tatatagttt cggggatcat cgtattgatt
     2101 ttagcagctt tactacttta tatcttagtt cggggaatac cgcatatttc atggtccttc
     2161 ttgaccgaac cttccaaagc ctaccaagaa ggcggtggaa tcgggatcca gctatttaac
     2221 tcgatttatt tgcttttgat cacgatgatc atcagttttc ctatttcttt aggtgcgggt
     2281 atctatcttt cagaatatgc tggaaaaaac tggctaacag atgtgattcg cacgtcgatt
     2341 gaaatcttga gctcgttgcc ttctgtcgtt gtcggtttat tcggtttctt ggttttcgtt
     2401 attcaattcc agtatggttt ctcgattatt tctggtgcat tggctttgac tgtattcaac
     2461 ttgcctcttt tgactagaac agttgaggag tctttacaag cggtccatta cacacaacga
     2521 gaagcaggat tggccttagg tttgtcacgc tgggaaacag tgataaaagt ggtggttcca
     2581 gaagcgacac cagggatttt gactggtgtg atcctaagtt ctggaagaat ttttggtgaa
     2641 gcagctgcat tgatctatac ggctggacaa agtgcacctg cgttagattt tacgaactgg
     2701 aatccattaa gtgtttcaag cccgatcagc attttccgtc aagctgaaac attagctgtc
     2761 catatttgga agatcaatac agaaggcacg atgcctgatg gtgtttctgt ttcggctggc
     2821 gcttctgccg tgttgatcat tgctgtttta ctgttcaact tcggcgctcg tgcgattggc
     2881 aaacgattat accgaaaaat gacttcagcc taaggagaag aagcaaaatg aaaaaatata
     2941 actggaatga aacaaatgtc atcaccatgc caaaagagaa agaagtagca ctatatacgg
     3001 atgatctaca tgtctggtat ggcgacaatg aagcaatcaa aggagtggat ctggaatttg
     3061 aaaaaaataa gatcactgca ctgattggtc cttccggctg cggaaaatct acgtatcttc
     3121 gttcgttgaa tcgaatgaat gatgggatcg ctaatacgaa aatcactggg aaaatcatgt
     3181 atcaaggcgt agatattaat gcgcctcaag tggatgttta tgagatgcgc aaacggattg
     3241 gcatggtttt tcaacgtcct aatccgttca gcaagtcgat ttatgacaat attacatttg
     3301 ctttgaaaca gcacggcgaa aaaaacaaaa agaaattaga tgaaatcgtt gaaacaagct
     3361 tgaaacaagc tgccttgtgg gaccaagtaa aagacagctt ggacaaaagt gccttggctt
     3421 tatctggcgg gcaacagcaa cgtttatgta ttgctcgtgc gatcgcgatg aaacctgata
     3481 tcttgctgct agacgagccg gcaagtgctt tggaccctat ttcgacagga acggttgaag
     3541 aaacacttgt aaacttgaaa gaagactata cgatcatcat cgtgacgcat aacatgcaac
     3601 aagcagctcg tatcagcgac tatactgcct ttttctatct aggaaaagta atggaatatg
     3661 acaaaactaa caagatattt accagaccaa aaattcaggc aaccgaagat tatgtgtccg
     3721 gtcattttgg ttaagacgga ggcctcaaca tgacaaaaga aattatttca tctaaagatc
     3781 ttcatctgta ttatggaaag aaagaagcac taaaagggat caatctaagt ttcaatcaag
     3841 gggaaatcac tgcgatgatc ggtccttctg gttgcgggaa atccacttat ctgcgttctt
     3901 tgaaccggat gaatgacctg attccgaatg taacgatcac cggaagtgtt ttatataaag
     3961 acaaagacat ctatggtcca aaaacagatg ttgttgaact aagaaaagaa atcggcatgg
     4021 tattccagca gccaaaccca tttccttttt ctatctatga aaatgtcatc tatggattga
     4081 aattaaaagg ggaaaaagat aaaaaagtcc ttgatcaagt agttgaagaa agcctgaagg
     4141 cagcgtctgt ttgggatgat gtgaaagaca aattgcataa aagtgcttta tctttatccg
     4201 gtgggcagca gcagcgtgta tgtatcgcac gtgttttggc agttaatccg gaaatcatct
     4261 tattggatga accaacaagt gcgcttgatc ctgtttctac tgggaaaatc gaaagtatgc
     4321 tgttagaatt aagagagcgg tatacgatga tcatcgtgac ccataatatg tctcaagctt
     4381 cacgtatttc agataagacg gcattctttt tagatggtca cttgatcgaa tacaacgata
     4441 cgaagaagat attcatgaat ccagaaaaac aagagacaga agattatatt tcaggtcgat
     4501 ttggataagg aggaaacgga atgctacgta cacaatttga agaagaattg ctgaatctcc
     4561 ataatcagtt ctacgaaatg gggatgatgg tgagcagcgc cgtgcacaag tcagttcgtg
     4621 catacatcaa acacgataaa atcttagccc aagaagttat cgaaaacgat ataaatatca
     4681 ataacatgga aacacgcttg gagaagaaaa gttttgaaat gatcgcattg caacagccag
     4741 ttacaactga tttgcgtatg atcattacgg tcatgaaagc tagctcggat ttagaacgta
     4801 tggccgatca tgcggtgtct gttgctaaat ccacgattcg cgtaaaaggg cagacgagaa
     4861 taccggaaat cgaaaaagag atctccgata tgtccgatta tgtgaaaaaa atggtggaca
     4921 acgtcctagt tgcttatgtc aaaacagacg aaaaagacgc acgtacgatc gccaatatgg
     4981 atcaaagagt caatgactat ttcaatcgaa tctataatgc aacaatcaaa aatatgcagg
     5041 aaaatcctga gacagtcatc agtggaacag actacttgaa tgttgccagt tatctagaac
     5101 ggatcggaga ctatgtgaca aacatctgtg aatggatcgt ctacttggct acagggaaaa
     5161 tcactgagtt aaatacgaac cacaatgaga ttttctaaac tgaagctgtg attatgccgt
     5221 ttgtttagga aaagaggttg tgaaatcagc ggttgtgaaa aagctgttct gcatcaagta
     5281 ataagaacag tcaaacaaaa aatagctcct catatttttc gtttggctgg acttattacc
     5341 gcagatgcaa gcttttgaac accgtttatt cgggaaaggg tttgtgacaa aacatgtcac
     5401 aaaccctttt catcatttca tagaaaggat aaagaatgca aaaaaaaatt agcatcatca
     5461 ctatattttt tatttgtgtg cttacttcat gggttattca gcatcaagct gctttatatg
     5521 aagtacctgt catcgagatc gaaaaagtac gaataaaaga ggatcatcaa ttgattacag
     5581 gaagactgat gaacacggat caaaaaggag aatcgattta tttatcggtt ccttatcaaa
     5641 aaaatcaaat agaaacagca aatataaaag cgagacaaat gtatctagtc caaatgcatg
     5701 gagatgaatg gcagctagta gcaaaaaaaa atgatggctg gcttttcttc ttcacaagta
     5761 tctccattgg cgccatgttg cttattggcg gaaaacaggg tctatttgct ttaggaagtc
     5821 tcatcatcaa tatcgtttta cttttacttt tgttgctcat ctttactaaa acagaaggga
     5881 tttctctatt aaatatatct atcctgttcg tcgttatagg gatgattatt agcatattgg
     5941 ctttagatgg atggaataga agcagtataa tcaaaatttc agctgcagtt acgagtgttt
     6001 ttttagcatt ttttatttgt cttttgacaa tgaatcattg gaaagaccaa ggactgcgct
     6061 atgaggaact gaattatctg acacgaccgt atcgttctgt ttttctttct agcgtactga
     6121 ttggaacagc tggaggaaca ctcgataccg ttatcacagt cattgcgaca ttagaagaat
     6181 tgactaagcg acaaccagaa ataacagcga aacagttatg gctttcagga aggcaagtgg
     6241 gtcgagatgt gattggctcg atgaccaata tccttttgtt cgtctatatc agtgggtcag
     6301 ttccatcgct tcttttatat ttaggaaatc aatggtcgtt caaagaaacg atagaacagc
     6361 atttatcatt ggaattttta cgcgtcattg ctggcagctt aggaatagta ttatcgatcc
     6421 ctatctcggt cctctttttt ctgactgtaa ggaggctgaa acgatgaatg tacttattat
     6481 acttgttggg attttagcaa tcagcgtgct acttattggc ggtacgcaag gtatgtatat
     6541 tcttttaggc ttattcatca atttagggat ctttttcctg ttgttattcg gttatcatca
     6601 gaagtggccg atactggcat tgtcggtcat aggattttta ttgattgctg tggttatctt
     6661 attttttatc aacggttata atctaaaaat gcgtgcagct tttgtgagta tactgatctt
     6721 tttattttgt ttcctactat tgcttcctat cactgatttt ttagcgatcc aaggttttac
     6781 atcggtagaa ctagaagaat tatctggctt agataagact ttagcgattg attttagatt
     6841 acttacgaga tcgttgcttc tgattagtct tagtggtgct gttttagatg ctagtgtagc
     6901 aatttctagt gggacattcg aagtatatca ggcaaatccg caattatctt tttaccaatt
     6961 gcgccattct agtttcgcta ttgctaaaaa aatattggcg tcaacagtaa tgacacttct
     7021 ttttgcattc atgggaagtt ctttagcgtt gatcctctgg tttattgatt tagacattcc
     7081 ttttcagcaa gtgatcaatg aaaaatcatt tgttctagaa tatacgatgg ctattcttac
     7141 cacattgtct gcactacttg ttcttccttt aacctgcgtg actacagcat atctctttac
     7201 aaaaaagaaa gaacaactca agatggagta aaagttacaa cttaagtcct atgacatttt
     7261 tactgaatca gtgcataata tggatgtaag gaaaagcttg caaacaattt tgcttaagga
     7321 ggcaatttcc atgaaagaaa gagaacgtat tttagattta gtgaaaaaag gaatcctttc
     7381 gactgaagaa ggcttagatt tattagaaag catggcaact gaaaaagacg aaaagcaaat
     7441 aaaaaaagaa gcagacaagg taactgctag tcataaagaa aaagatcagg caagtcaatt
     7501 gatcgataaa cttgaaaacg gcgaagaaga aatttctgag ccagttgatc caaaggaaag
     7561 agaaaggcaa gatcaagaaa acttagaaaa gatcttagat gaactagcaa ctgaagcgaa
     7621 taaaacttcg gcgcgtctag atgaagtaaa tgctcaattc gctgacaata aggcagcacg
     7681 caatgagaaa caggaagcat tgatgcagct gaatacaaaa gaagaattag gcgagttgac
     7741 agaagaagaa ttagcacagc gtcagacgtt agaagcagaa atcaaagaat tagaagctag
     7801 tggcgataca ctgttagaag aacaattgaa attagaagca gaattaaaag atatccgtaa
     7861 gaatcaatgg tctgagaaaa aagaaacgtt tactgataag tttgaattgc cagatgattg
     7921 gaaagatcaa gcaacagata cactgaatca agtaggcgaa aaaatgtcag aagctggatc
     7981 acagttaggc aaattcttga agaaaacatt ccaaactgtt tcagaaacag tgaatgacaa
     8041 catggaatgg aaagatgtca gcttacgtgt ccctgggatt gcgacaacga aatttgagca
     8101 tgaattttac tatgaagctc ctgcagcaag tatccttgat atcaaggcag caaacggaaa
     8161 cgtgacgtta aaaacgtggg acagcaacga tgtgaaagtt gaagctaaga tcaaacttta
     8221 cggaaaaatg ggtgctgaac catttgaagc attctctgaa cggagccaga ttgaggttaa
     8281 tgaagatcat atctccttcc aaattccaaa taaacgtgtg cgtgcagacc ttgttttcta
     8341 cttacctaaa cgagtatacg accacgcagc tattaaactt ctaaatggca acatcatgat
     8401 tgaaacattg gaagctaaag atatttatac aaaatctacg aatggtaaca tcatcgtgga
     8461 ccaattaact gcaacgatgc tggaagtaga aggtgtaaac ggcaatatcg atattcgaaa
     8521 cgggaacatc ttagattcta ttatcgaaac agtaaatggt acagtgacat ttggtgcgac
     8581 agcagaaaac ctttctgtgt ctttggtaaa tggtgatgta cgtcttacga ttaaagaaga
     8641 taatttgaaa aaagtagaag caagttcagt gaacggaaat gtcaaagtcg ctttaccaga
     8701 tggcatcggt ttggaaggac atgctaaaac aagtcttgga agtatcaaca gccgtctgtc
     8761 caattacgaa gtagttcgtg agaaaaaaga acggacaaac caaatgttgc aattccgtcg
     8821 tgtttcagat ggtgaaatca cgcaagtgca actttcaacg acaacaggaa gtatctattt
     8881 aaaagataca gataaataaa gaggttgtga aagagatgtt cagccttgag caataagaag
     8941 cgattttgta aaatagcttc ccctatttta caaaattgtg ccttattgcc gataggctat
     9001 ctcttgaaca ccgtttacca agagattatg aacctgccaa ttatcgccga gtcacaagga
     9061 ataatttcag aaaaagctcg tcacgtttta tgaatatggt aatttgtgac cgagaatttt
     9121 gcagaaagac accgattaag aagaaggttg tcacaacgat tttcttcatt taaaatagta
     9181 gaacagaggt gagacatgat gaaaaaaaag ttgacaaaat ccaataaaaa tgttgttttg
     9241 acagggactt tagcaggaat aggagaatac atcgggattg atccgactgt tatccgtgtg
     9301 ctttatgtat tcctgagctt agttgctttt ggaagtccaa tcattttata cattttatta
     9361 gctttgatta tcccatctgc gcgttctgca cgaacaaact atggacataa caatgcatat
     9421 tatgaaaaaa acaattatcg tgaacaaact cgtgaacaaa caaaaaaacg caaagaagcg
     9481 gaaaaaatcg atgatgacga atggagtgac ttttaatgtc gtactttcaa cgtattttag
     9541 ttaatacgtt aacattcata tcattggctg ttctttttcc tgcgatggtc catgtgagca
     9601 gtatttggac ggccgttctt gccagcttcg tattgtctgt gttgaatatg tttgtcaaac
     9661 cgattttgac gatactgtcc ctgcccttta cattagtcac attgggattg tttacgtttg
     9721 ttatcaatgc agcgattctt aaaatgacct ccttttttgt gggagcagaa cattttagtt
     9781 tttcttcatt cggtgcagcg ttgttagtag ctatcttgat gtcgcttgtc aatgtaattg
     9841 tcagtgaaca taacagtaga aaataaaaga aagctgtgcc gaactatcgg cgcagttttt
     9901 ttatttagaa tcaggaatgc gttttctatt acattggtaa ttaaataaaa acgttttttt
     9961 taattggtag tacttttggc tagacaatga taaaatgata gagaacactc taatagaaat
    10021 gaggtagcat gaatgtcaga agttgtaaaa gttagccaat tagtgaaaaa attatctttg
    10081 gaaatcgtga caggcgacga gaaaagctta gaccgtgtca tcgtaactgg ggacatctca
    10141 cgcccaggac tagagttgac tggttatttt aattattatt cccataatcg tttacagcta
    10201 tttggcagta aagaaatcag ttttgcggaa cggatgatcc cagaagagcg tttgatcatc
    10261 atgagaagat tatgtagtga agatatgccc gcatttatta tttcaagagg attaccggct
    10321 ccagaagaaa tgatccaggc agcaaatgaa aatgggatag ctgtactgcg ttctcccatc
    10381 tcaacttctc gtttattagg agaattatcc agttatttag atggtcgcct tgcccctcga
    10441 acaagtgtcc acggtgtttt agtggatgtt tacggattag gtgtactgat tcaaggtgac
    10501 agtgggatag gaaaaagtga aacagcgttg gagttgatca aacgcggtca tcgattgatc
    10561 gcagacgatc gagtagatgt ttatcagcaa gatgaattaa cggtgatcgg agaaccgcct
    10621 aaaatcctgg aacatctaat tgaaatccga ggaatcggga tcattgatgt aatgaatctt
    10681 tttggtgcga gtgcggtacg tggctctatg caagtccagt tggctgttta tctggaagca
    10741 tgggcaaaag ataagaaata cgaccgtctg ggctctgaag acacgaccgt tgaaatcgcg
    10801 gaagtgggca ttccacaaat aaaaatccct gtcaaaacag gacgaaacgt tgcaatcatc
    10861 attgaagtag ccgcgatgaa tttccgtgca cgtacaatgg gctttgacgc aacgaaaaca
    10921 tttgaagaaa gattaagtcg cttgattgaa gaaaactcag gcaacgacta gcaaggagtg
    10981 acaatatgtt agcacaaatc aatcgagttg cttttgagct tgtagggatt cctatctact
    11041 ggtatgcatt gatcatcgta tcaggtatca ttatcgccat gtggcttagc agcagagagg
    11101 ctgttcgggt aggattaaaa gcagatgatg tgacagattt tatgttgtgg ggattgccta
    11161 tctccattat tggtgctcgc ttatattatg ttttatttga tcttccccag tatatagctg
    11221 atccaatcca aatattcaat atccgttcag gaggcttagc tatttatggc ggactgattg
    11281 caggcgcgat cacgctgtat ttttttacta aatatcattt tatttctttg tggacttttt
    11341 tagatatcgc tgcaccaagt gtattattag cacaggcaat cggtcgttgg ggaaatttca
    11401 tgaaccacga agcatatgga ccagacacga cgcgaagctt cttagaaagt ctgcatttac
    11461 cgcaattcat cattgataat atgtatatcg aagggatcta tcgtcaacca acatttcttt
    11521 atgaatcact ttggagtttg attggattta ttttgctttt gtttttacga aagaaaccac
    11581 accttttgaa aaaaggcgaa gtccttttat gttatctaat gtggtatagt ttcgggcgtt
    11641 tctttattga aggattacgt acagacagtc tttacttttt aggtgtgatc cgtatttctc
    11701 aattattatc tgctgtgtta tttgtcggta cattgattct atttatttgg cgcagaaaaa
    11761 aaggaaattt accggattat gaccgctcat taggaaaaaa tcaacaaatt atctgatttt
    11821 tagagcgtta aaaatgaaaa aaatttcagg agtggtaaaa tgaattatag agtagcggtt
    11881 ttaggaccag gctcttgggg tactgctttg gcacaagtcc tcgccgaaaa cggacatgac
    11941 gttcggattt ggggacatcg tgcttcacaa gtagaagaaa tcaacaccca acacacgaat
    12001 caacgttatt taccagatca ttatctaccc gattccatcc aagcattcac caatatggaa
    12061 gatgcagtaa aagacgcaga tgcggtgcta ttcgtcattc caacgaaagc aatacgttct
    12121 gttgcaaaag aactagtgcc taaattgaat acaaagccag tgatcatcca tgcaagtaaa
    12181 ggattagaac agggaacaca taaaaggatc tctgacatct tgttagagga aatcccagca
    12241 gaaaaaagaa aagctgttgt tgttctttct ggaccaagcc atgcagaaga agtagcgaaa
    12301 catgacatca ctacgatcac agctgcaagt acagatgcaa aagcagcaaa atatgtacaa
    12361 gagctgttca tgaataatta ttttcggatc tataccaatc ctgatgtgat cggtgtagag
    12421 atgggggcag ctttgaaaaa tattattgct attggtgctg gcgctattca cggcttaggg
    12481 tttggcgata atgcaaaggc agcaatcatg acgcgcggat tagctgagat cagccgttta
    12541 ggtgtagcaa tgggtgccaa cccgctgacc tttatcggat taagcggagt aggcgatcta
    12601 gtcgttactt gcacaagtgt tcattctagg aactggcgcg caggcaactt gttaggacaa
    12661 gggcagcccc tagaagaggt gctagccaat atgggaatgg tcgttgaagg tgtgaatact
    12721 actcaagcag cgatggaact agcccaaata atggaaatcg aaatgcccat cactcaaaca
    12781 atctatgaag tgctgtatga acaaaaagaa atcaaaacag ccgcaaaaga aatcatgtta
    12841 cgtgatggga aaaaagaaaa tgaattctca ttaacaattt tttgaaggag cgaatgtata
    12901 tgaaagtcaa aaaagcagtt attccagcgg caggattagg aacaagattt ttaccagcaa
    12961 caaaagctat ggcaaaggaa atgttgccaa ttgttgataa accaacaatc cagttcatcg
    13021 ttgaagaagc gttgaaatca ggaatcgaag acatcttgat cgttacagga aaagctaaac
    13081 gaccaatcga agatcacttt gattcaaata ttgaattaga aacaaaccta agtgaaaaag
    13141 gaaagaccga attattgaaa ttagtagaag aaacgacaga tgtcaacttg catttcatcc
    13201 gtcaatctca tccaaagggt ttaggacatg ccgttcttca agcaagagcc tttattggaa
    13261 atgaaccgtt tgtcgttatg ctaggcgatg atatcatgga agatgaagta ccattgacac
    13321 agcaattaat cgatgattac gaacgtactc atgcttctac gatcgccgtt atgcaagttc
    13381 ctcatgatga aacttcaaaa tacggaatta tcaatccagg agaagtattg gaagatgggt
    13441 tatacaatgt caaaaacttt gttgaaaaac cagatccaag caaagcacca agtgatttag
    13501 ctattattgg acgttactta ttgactccag aaatttttga tgtattgtcc gagcaacaac
    13561 caggagccgg aaatgaaatc caactaactg acgcaatcga tacattgaat aaaacacaac
    13621 gcgtatttgc ccgtaaattc acaggtaaac gttacgatgt cggtgacaaa tttggtttta
    13681 tgaaaacaag tattgaatat ggtctaacac atccagaagt aggtgcacct ttacgtgagt
    13741 atatcctgtc tttaggtaaa aaattagaag cagaaggaag caagatcgat cataatccaa
    13801 aaggaataac agaaatcaaa gaagaaaaga aaaaataacc cttttttcta acgagcttca
    13861 gctgctaatt gaataaaaag tagttgaagc tcgtttttat ttgtttaaaa aacacgaaaa
    13921 tgctatcctt atagaaaaga gagacaagaa gggagtttga tcatgtcagg aggagaaatt
    13981 gcaggattga tcgcagcaat cgcctttgcg atacttgtcg tttttatcgt tcgtgtgttg
    14041 ttgcaagttt ctaaaacttt agaaaaagta gatcagactg tagcagaagc aaatacgacg
    14101 atcgaaatcg tcacaaaaga cgtagatctt ttgtctagac aagtagaagg cttgctggtc
    14161 aaaagtaatg aactgctctt ggatatcaat ggaaaagtcg caacgatcga tccattgttc
    14221 acagcagttg ctgatttaag tgaaagtgta tcagaactta atcattctag taaaaacatc
    14281 gcgacgaaag tcggcggaat cggtaaaacg acagctaaag cgacggttgc cggaaaagtc
    14341 ggtgggacag cgatgaaatt ttttaaaaac aaaaaacata ctgaaacgac aggaggaaaa
    14401 taaaaatggc taaaaaaggt ggatttttat taggtgcagt agtcggagga acagccgcag
    14461 caatcgccgc gttattgtta gcaccaaaat ctggtaaaga acttcgggat gaattagctg
    14521 cccaagcgga tgatttgatg gatatggctt cagattacac agatatggcg aaagaaaaat
    14581 cgactgagtt aacaggtatt gcaaaagaaa aagcgcaaga tctttctcaa caagcaggtg
    14641 atctagcagg tagtgtcaaa caaaaagtaa gtgaatcagc agatgaagca ggaagtgaat
    14701 ctgacgatat cttgttaaat cttaaagaac aatcagctga cttatctaat cgtttcaaaa
    14761 aaacggcaga agatatgaaa aaacctgcag atgaattagg aagtattgct aaagatactt
    14821 cagaagatat catgattgat gtaaaagacg ctgctggtaa agcaggagat actgtcaatg
    14881 aaggaatgtc agaagcaaaa ccagttgcag atgacacaaa agaaatgatg aaaatggcaa
    14941 aagaagatgc gaaagaagct aaaaaagaaa taaaagatac aatgacagat tcatctacaa
    15001 atcactaaaa tcactaaaat cactaaaatc acaaatttat agaataaaaa ggtgggagcg
    15061 gactagctct cacctttttt actcttattt ttctaaaact agtaattctt ttggtgtagt
    15121 cgtgaatggc acacagccat cgcttgtcac atatacacag tcttcgatac gtacccctac
    15181 tttttcagga atatagattc ctggttcaag agagaagcac atcccttctt cgattactaa
    15241 gtcatttcct tctacaaggg aagggaattc gtggacggtt gttccgatcc cgtgacctaa
    15301 acggtgattg aaatattcgc cgtagccata gctgttgatc acgtttcgtg cgatttgatc
    15361 aagttcacct gctgtcacac ctggacggac ggcttcttgt gcggcaagct gtgcttcaag
    15421 agtgatgttg tatatttttt cttggaagtc ggttggtttt tgataagcaa ttgtacgagt
    15481 agcatcactg caatagccat tccaaacaac gccaagatcg aataacacaa gttcattcgg
    15541 tgaaatcttc gtgtttcctg gtgtaccatg agggcttgca gcatttgctc ctgcaaggac
    15601 taatgtgtcg aaagacattt gtgaaacacc ttgttttttc aattggtatt cgatttctgc
    15661 aatgatcgct tgttcagtca cgccttcttt cactgcttta aagccaatct caaatgcaac
    15721 atcggcccat ttaccagctt cgatcagttt gtctttttcc tctagagttt tgatcagttg
    15781 tagacgttgg atgatggaag ctagatcagt gtaagaatcc gttcccgtca agatatcaga
    15841 gatagcatcg aagcgctcga cagtcaaggc gttcttttca atccctacat tgccaggctg
    15901 accatagcga gcacgaagtt cttttccgat gatctcccaa gggttttcgc tgtctaaata
    15961 gccacggaca tcatacgccc aactactgtt ttgagcatct tcaacttcca aagcaggagt
    16021 gaaaaggaaa ggatcttgtt caagagggat aaaaagagct agtacacgct catgaggatc
    16081 actttcgtat ccggaaaagt aagcgatgtg gccagggtcg ctgatataag ccaattcgac
    16141 ttggtgttgt gccatccatt gacgtagttc attgattttt acgttattca tttggcaacc
    16201 tactttctaa attaattttg tattcaataa ttgtaccact gatttattaa aagaccaacg
    16261 ttctaaaaac tgaaaaaagt aaaaattttc atgaaaacag agataaaacg gttgcaattc
    16321 ttgagattat ttgatattct attttagaaa tgtaagacgt attttcagaa aacatataaa
    16381 atatgaatag gagaacacaa atggaaaagc aaacaatcac tatctatgat gtagcgagag
    16441 aggcaaatgt ttcgatggct actgtttcac gtgtcgtaaa tggcaatcca aacgtcaaac
    16501 cagcaacaag aaaaaaagtc ttggaagtta tcgatcgctt ggattatcgt ccaaacgcag
    16561 ttgcgcgtgg cttggcaagt aagaaaacaa caacagttgg ggtaatcatc cctgatgtca
    16621 gcaatatgtt ctttgcttct ttagcacgtg gtatcgatga tgtggcaaca atgtacaaat
    16681 acaatattat cttagcaaat tcagatggta acgaccaaaa agaagtcaat gtattgaata
    16741 acttactggc taagcaagtg gacggcgtga tcttcatggg tcaccatatc acagacgaaa
    16801 tccgtggaga attttcacgt tcaaaaactc ctgttgtatt agctggctct attgatccag
    16861 atgaacaagt aggtagcgta aacattgatt atgtatcagc aacaaaagat gcagtaaaca
    16921 aattggcaaa aagcggcaat aaaaaaatcg cttttgtcag tggtgcgttg atcgatccta
    16981 tcaatggaca aaatcgtctg aaaggctata aagaagcatt aagcgaaaat aacctaccat
    17041 atagcgaagg attgatcttt gaagcacaat acaacttcaa agatggtcta tctctagtcg
    17101 atcggatcca taatagcggt gcaacagctg tctatgtttc agatgatgaa ttagccatcg
    17161 gtattttgga tggtttatta gaccgtggcg tgaaagtacc ggaagaattc gaaatcatca
    17221 caagtaacaa ttcactactg acagaagtag ctcgtcctcg tttaacaagt atcacacaac
    17281 cattatacga tttaggggcg gtatctatgc gcctattgac gaaaatgatg aataaagaag
    17341 aagtcgaaga aaaaacgatt atcttgcctt atggaatcga agaaaaagga tctacaaaat
    17401 aataaaagga caaccattca gaggttgtga gcctgccgtt taactctgcg ttacaaggag
    17461 caatttcaaa aaacagcatt tcatgtttga tgaatattgt gactcgtgat tcggagttgg
    17521 caggagaaca ccgtttattc gggaaaaggg gttatgacaa aattttgttt aatccttttt
    17581 ttttgtctta aaaaagagag aaagtggttg aaattacatt tatcgtatat aaaacaatcc
    17641 gtgtatagat gctgcattat gttcaccacc atcaaaaaaa taagggactg tgacaatact
    17701 attgtcacag tcccttatta ctgtgtattc tattaaatca atcatctttc tcatctgatt
    17761 ctttggttgc ttgattcgct cttgcataag ctgaagcggt gttccagtaa tctgtatagt
    17821 tttcatcggt accgccaacg ccaaatttaa aggcgctttt cttcggtcca tttttcgccc
    17881 atagcgattc aactgttttt cctggtgttt ggatggattt tttattgaca gtaacttttc
    17941 caggtgtttg accagtaaat ccagatactt ttttcttcat cacatcagat gataattcga
    18001 atttctcatc agtgccaaaa atgtcaggtt ttgcttggta agcttgattg atcagattgg
    18061 caagataatt agccgaacgt atccttgctt tgctgtccat tccagtattg tcatcgtgcc
    18121 ctgcccagct actgatggta atagacggag tagagacaat tagccatgaa tctcggtatt
    18181 cattggtaga accagttttt ccgacccaat ctgcttttcc tagatttccg tttaagcttg
    18241 agatggcatc tttgaatggt gtagtgattt ttgcattgat cacactgcgc atcatgtcat
    18301 tcatgatcga tgccgttgct tccgaataga tccggacaga gttgctttcg tgtttataaa
    18361 ttacatttcc tgcgttatct gtgatggaat cgatgatata gccttcttca tagacgccgc
    18421 cgtttgctaa agcctgaaat ccattggtct gttgaagcgt cgtgacatct actgtaccta
    18481 aaggagcaga ttcaacgccc caattgtcat ttgccgggta gttcatcttt gccaagtagt
    18541 gctcgtaagc atattgtttc gatccaccgg aattcaacac atcttgatat aagtgataag
    18601 ccgggatatt gttggaccat tctagtgact cacggacggt ttggaaggtg ttcgatcctt
    18661 cgttagtagc attgacgatt ttctcgccag cattttctcc ttcttgccaa gttgttgcat
    18721 aatctgatac acgggattct gatccgataa gccccatgtc gattgcaggt ccataaacaa
    18781 gaactggttt gattgatgag ccagcttgac gttctgtatc gaaggcatgg ttattttgat
    18841 tctgactgta attccgtccg ccaacgaatc cgtagatgcg gccagttctg ttatccatca
    18901 atacatttcc ggtttctact tgactgccag caccatcatc taacatgtat ccataatttt
    18961 ctacgccatt ttgcaatgca gcatagacat ctttatcgat tgtcgaatgg attgtatatc
    19021 ctttattttg gatcgtttgc tgtgcctttt gataataagc gtcactgacc gatgaatcag
    19081 acatatcagc gttgtctttt tcagccaatt gttgtgcaat gattcttgtt gcttcattca
    19141 tcacagtata atacaagaat tctctgtcag tctgttctgc gatttgttgt ggtagaaagt
    19201 cttttgtaag gtcataggct tttgcttctt cgtattgttc tttcgtaatt tggccttcac
    19261 gatacatact gaataaaaca aaatcttttc tggcaaggcc ggcagaaaga tcttctttta
    19321 gcgcacctgt gttcgtgtat ggactatacg tgatcgggct ttggggtaat ccagcgatat
    19381 aagcagcttg cggcagcgtc acatcttttg catctacacc aaagattcct cttgcagctt
    19441 cctggactcc agcaatgttt tgtcccttat tattacgacc aaatggtgag acattcaaat
    19501 acgtagcaat gatctcatct tttgaaaagt atttttctac ttgtgcagaa agaatgatct
    19561 cgtttgcttt acgtttgaat gttgtttcat ctgtaaggat ctgctgtttg actaattgct
    19621 gtgtcaaagt tgatccacca gaagagccaa taccagtagc ttcagatact aatgctcgta
    19681 aaacagcttt aggaacgtaa ccttggtgtt tatcgaagta ttcatcctct gtgctgataa
    19741 ttgcagtttt caataaaggt gaaattttat cagatgaaat agtcgtgcgc atcagatctg
    19801 tctggatttt tgatatctct gtattatctg cataaacaat ctttgacgtt tctgtaatgt
    19861 ttcctagttc tgtctgcagt gcttttttag tcggcaattg tgtgtcttcg acgagatagg
    19921 caaaatagcc agcgcctatt ccaataccta gagctgcgaa catcaaaaga agaacagtaa
    19981 taaaaacagt caatgattga aaaactctaa aaataatctt aggaacaaac catttttctt
    20041 tttttcttct attctgtttt tttcgagtat ttttgcgtgg caaaagggtc acctcaacta
    20101 atggttttat ttcgctaatt atagcaaata atagaacaaa aaaacagaca aggctgtttt
    20161 tggcaggaat ttctttttta aaagagttaa aacgtgagaa aatgatcttt tttataaaag
    20221 agttcttaag cttttcttta gagaaaattt cagttactat ggagaacagg gcaaagaaaa
    20281 atcaatcatt ttctttgtat atcatcttac caatatatag gaattgttag taacaattgc
    20341 ctttcaagga ggaatccatc catgttatta ggctcacacg tcagcatgag cggaaagaaa
    20401 atgctgttag gagcagcaga agaagcagcc agttatggat cttcgacttt tatgatctat
    20461 acaggcgccc ctcaaaacac tagaagaaaa gccatcgaag agatgaatat tgatgccgga
    20521 aaagcgtaca tggcagaaaa cggtctaaca aatatcgtcg ttcatgcccc ttatatcatt
    20581 aatttgggga atacgatcaa accagaaatg tttccttttg ccgtccaatt tctgagagaa
    20641 gagattgttc gtgcggaagc tttaggcgct actcaaatta cacttcaccc aggtgctcat
    20701 gtaggggcag gaacagaagc cggaattgct agaattattg aaggcttgaa tgaagttttg
    20761 acaaaagaac agacggcaca aatcgctttg gagacaatgg caggtaaagg tacggaaatc
    20821 ggtcgaacat ttgaggaatt agcgcaaatc atcgatggag taaaattgaa tgaaaaatta
    20881 tccgttactt ttgacacctg tcatacgaat gatgccggtt ataatattaa agaagacttt
    20941 tcaggtgtaa tggaagagtt tgataagatc atcgggttag atcggcttaa agtactccat
    21001 atcaatgatt ctaaaaatcc gcaaggttcg cataaagacc ggcacgccaa tattggtttt
    21061 ggaacaatcg gattcgacac gctgaataag atcgtccatg atccaatgtt tgacaatatt
    21121 tcgaaaatct tggaaactcc ctatgtcggc aaagataaaa agaaagctta cgcaccatat
    21181 ggaaaagaaa tcgccatgtt caaagcacaa atattcgatc cagaagtgtt tcctgagtta
    21241 ttagcagaag aagtgatact ctaagatttt ctatctgaca gcagcaaaaa aggaccaggc
    21301 acattgcctt gtcctttttt gtttaagaaa caaaggagtg ctttttgcag tctttttcat
    21361 catgcatctt ctcccccctc tttttaaatt tagacaaaca gggtatactg gtagaaaagt
    21421 aaaaaaacga aaagtttcac aagcaagtgg caaaacgtga ttggaaggac gaaaagatgc
    21481 tgaacacttt aaaacccttg atcaaccaac taaaaaagaa acatacatac tttattcttg
    21541 gcatagccct attatttaca attattcgtt tgtatttata tagtaaagca atctatggaa
    21601 cagacatgtc ggcaacctat gatgaccagc tgctgattcg ctatgctcaa aatattttgg
    21661 acggtaaatg gctaggggag tatacgacta caacactttc caaaggaatc tcctacagct
    21721 tatttttagt acttgcacat aaactgcatc tttcttatgc agttttgttc ggcatgttca
    21781 gtatttttgc cagtatattg gttgcactcg cgtttaaacc gatcgtgaag aacaagtatc
    21841 ttcttttaag tttctatttt tattttttgt tttctccgat tagttttaca catgaatatt
    21901 ccactcgaac gtatcgtaat gccctcgtga ttccggctgt tgagatcatt atcgcttgtt
    21961 tactagctat ttattaccgt aaatatagtt ccagcaaaaa aatgctgccg tggtttttag
    22021 ggctagctgt ttcttttcct ttcttttggt tcattcgcga agattccatc tggcttcttc
    22081 cttttactgt agtcgcgtta ggcatcagtt tcgtccagat cttgctagga caagactttt
    22141 ctttcaaaga agcaaagaag ttaaaagaac gaccatttct cctttcgaaa caagaatggc
    22201 tgaaactttc attgttttta ctccctctag tcggtttctc catcgtctat cagtggatcg
    22261 aatataaaaa cgagacaaca tatagtattg ccgccatcaa tgaccgttcg agtggcgaat
    22321 ttggacgttt gatgaaacag ctgatccgaa tagatgacga aacgaactta aataaagaaa
    22381 acagtgagat atgggtctcg catgctgcat taaataaagc attagacgca tctcctacat
    22441 ttcatgctgt ttctactcgt attgacgaat tgtatacgac tcatgcgtgg acccaaggtg
    22501 gaaaagtaaa agaattacca ggagatatca ttttttgggc attgcgagat gtggtaagcg
    22561 aatctggtta ttaccaaaac aatgcaaaag aaaccaatga tttttggaaa aaagtcaatc
    22621 aagaattaga agaagcctat caaaagggaa ctatcaaaaa gaaaaaagaa ttctatctga
    22681 tgggatcagg ggatggaaag acgatgaatg atctgccaat cgtatttgct tttctgaaat
    22741 caggatatgc ctatgcatgg cgatacaaag atttccatca aggggggaat tatagtttcg
    22801 gtacgcaaga acaagtcgaa caggcccaag aattattgaa tcttccacta atggataatt
    22861 ggatcgatag cagtgatccg agaacacctg atttccagtt gacaaaaaca gcaaaagtcg
    22921 cgaatgtcat gataaaaatt taccaagttc tggccattcc cgtgctgatt gcttcaatag
    22981 cagggatatt acttatgata ctaggaaccg catttgcaaa agctgatcgt gcctattata
    23041 gttcctcttt gattgtagtc accggattaa tactgacaca attggttttt cttttcggcg
    23101 tctcgtggtt ttgttcttat gcgccagaaa aaaaggatta ttttttaagt gtctacactg
    23161 gcgcaggcgt gccattaacg caattcatcg tagtaacaag catgctagtt ttcagtaaat
    23221 tgaatctgaa aaaagcttta tcttccaaat aaaaacatac taagattaga ggagaacatc
    23281 attgacgaat gatgttctcc tctattttct atttttagat tatagtgaag agtgaaagaa
    23341 agttcgaaca aagccttgag gactatgagt ccctatcaaa aaccgaactt ctttactgtc
    23401 attttagaat caggtttacg tatgttgggg ccatgagccc gtgtatagga aattggaatc
    23461 gaaatgaagc agtaaagttc tttcaaaact taaaaaggag gaaaacaaat gccttacatt
    23521 cttgcattag atgtttcaat gggacacagc tacagtgtcc tatacaaaga cgacacctgc
    23581 ttatttgaag acgagatcga acacactcaa agaggatttc atgcattact cgaagagatc
    23641 cagcacttgc ctgaacgtcc attgattgtt tttgaagcaa cggggattta ttccagacca
    23701 gtcgaaaagt tctttcaaga tcatcacttt tcttactgtt tgttaaaccc gttagaagcg
    23761 aaaaaacaga tggaagaaaa caccttatgc agttggaaaa cagataaatc ggatgcccat
    23821 cggctcgcac agactcatct taaaaatcaa cgtcagccaa aagaagtaca aagcaatttt
    23881 tacttagaga tgcgcgattt agcccgtttt tatcaagaaa ttgagaagaa aatcactcgt
    23941 ctgcgcatgg atttacacaa ctgccttcag ttgacttttc cagagcttga acagttcttt
    24001 ttaaacaggc tgacccctta cgcattgata ttgatccgtc tttttcccca tccagatttt
    24061 gtattggcat ctactcggac aaaaataaaa aataaactga tcaacgaaac acggaaaaaa
    24121 atctccgcga atcgtgccga gcaaaaggca gatcaaatca tccactatgc acagtgtgcc
    24181 tatccagctg ttgaaaaaga cagtatccac tgtcaaaaaa cgatctatta cgctgaactt
    24241 ctccaagatt tactcgaaca aaaa
//
LOCUS       DANKKG010000030        23830 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000030 DANKKG010000000
VERSION     DANKKG010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23830
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.030"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(54..788)
                     /locus_tag="PW004_001019"
     CDS             complement(54..788)
                     /locus_tag="PW004_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HDL2726007.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1115..1525
                     /locus_tag="PW004_001020"
     CDS             1115..1525
                     /locus_tag="PW004_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HDL2726008.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1518..2219
                     /locus_tag="PW004_001021"
     CDS             1518..2219
                     /locus_tag="PW004_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HDL2726009.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2219..3805
                     /locus_tag="PW004_001022"
     CDS             2219..3805
                     /locus_tag="PW004_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2726010.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3824..4045
                     /locus_tag="PW004_001023"
     CDS             3824..4045
                     /locus_tag="PW004_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HDL2726011.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4042..4803
                     /locus_tag="PW004_001024"
     CDS             4042..4803
                     /locus_tag="PW004_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HDL2726012.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5183..5692
                     /locus_tag="PW004_001025"
     CDS             5183..5692
                     /locus_tag="PW004_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HDL2726013.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5767..6306)
                     /locus_tag="PW004_001026"
     CDS             complement(5767..6306)
                     /locus_tag="PW004_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HDL2726014.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="PW004_001027"
     CDS             complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="PW004_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HDL2726015.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7319..8170
                     /locus_tag="PW004_001028"
     CDS             7319..8170
                     /locus_tag="PW004_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDL2726016.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8198..8833
                     /locus_tag="PW004_001029"
     CDS             8198..8833
                     /locus_tag="PW004_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HDL2726017.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8853..9626
                     /locus_tag="PW004_001030"
     CDS             8853..9626
                     /locus_tag="PW004_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL2726018.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10034..10831
                     /locus_tag="PW004_001031"
     CDS             10034..10831
                     /locus_tag="PW004_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HDL2726019.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10809..11222
                     /locus_tag="PW004_001032"
     CDS             10809..11222
                     /locus_tag="PW004_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HDL2726020.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11206..13851
                     /gene="mutS"
                     /locus_tag="PW004_001033"
     CDS             11206..13851
                     /gene="mutS"
                     /locus_tag="PW004_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDL2726021.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13869..15977
                     /gene="mutL"
                     /locus_tag="PW004_001034"
     CDS             13869..15977
                     /gene="mutL"
                     /locus_tag="PW004_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HDL2726022.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRQTGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15999..16559
                     /gene="maf"
                     /locus_tag="PW004_001035"
     CDS             15999..16559
                     /gene="maf"
                     /locus_tag="PW004_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HDL2726023.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16644..18632)
                     /gene="treP"
                     /locus_tag="PW004_001036"
     CDS             complement(16644..18632)
                     /gene="treP"
                     /locus_tag="PW004_001036"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HDL2726024.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18855..19568
                     /gene="treR"
                     /locus_tag="PW004_001037"
     CDS             18855..19568
                     /gene="treR"
                     /locus_tag="PW004_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HDL2726025.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19645..20091
                     /gene="msrB"
                     /locus_tag="PW004_001038"
     CDS             19645..20091
                     /gene="msrB"
                     /locus_tag="PW004_001038"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HDL2726026.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20261..20863
                     /gene="ruvA"
                     /locus_tag="PW004_001039"
     CDS             20261..20863
                     /gene="ruvA"
                     /locus_tag="PW004_001039"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HDL2726027.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20876..21877
                     /gene="ruvB"
                     /locus_tag="PW004_001040"
     CDS             20876..21877
                     /gene="ruvB"
                     /locus_tag="PW004_001040"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HDL2726028.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21906..22484
                     /locus_tag="PW004_001041"
     CDS             21906..22484
                     /locus_tag="PW004_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2726029.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22536..23018
                     /locus_tag="PW004_001042"
     CDS             22536..23018
                     /locus_tag="PW004_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HDL2726030.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23135..23509
                     /locus_tag="PW004_001043"
     CDS             23135..23509
                     /locus_tag="PW004_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726031.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7781 a         4286 c         5115 g         6648 t
ORIGIN      
        1 taaaaaaact tggtacgcaa tcgctgtacc aagttttttt atttagcttt tgattaagcc
       61 ataaataata atttatataa gaatactgct acgacaccag cacagatagg tgctacaact
      121 ggtacccatg catatcccca ttgagaagaa cctttatgtt tcaatggaag aagtgcgtgt
      181 acaagacgtg gagcaaagtc gcgggctggg tttagtccag gacctgtagg accaccaagt
      241 gaagcaacta atgtccatac aaggaaacca agtgctaagt gagctgtacc aagattatct
      301 tggaagaatg gtgcttttgt aatacctagg gcaccaaaga ataaaacgaa tgaaccaaag
      361 aattcattga cgaaaccatt gaatttagaa ccaacgctgt tgattgttga gaatgtacct
      421 aacacatggt ttacgtcttc tgtttggtcg tagtaaggtt tgtaacaagc tactacgatc
      481 aattgtccaa tcgctgcacc aatcaattgt gcaatgatat aaggaacaac ttcagcccaa
      541 gggaatagac cattagatgc taaagcaatt gtaaatgctg ggttgatgtg gttaccgctg
      601 attccaccaa aaatcattgc tggcatcata accccgaatc catacccgac agcaatcagc
      661 atccagtcac tgccatatcc ttttgtacct tttaaatcta cgtttgctac agcaccgtta
      721 cctagaacga ttagtaatgc tgtaccaatc atttccgccg caagacgggt cattagtgag
      781 taatccataa atacctccaa aagttataaa aatgcaaaaa aaaaagaaac atcaacacga
      841 aaagcatcgg tgcagtgttt cgtgtcggaa ggatttctgc agtcttaaca aatgtgaata
      901 aaaaaatgaa ttccattact tataagaaac cagaccaaca gtaaaactcc aactcttttt
      961 aaaagttctc cacaaatcac caagcttaac tataaatata ccaaaaaaaa aacgtttttt
     1021 ttctcaaaaa ttgtatctta gaaacagcat gttttgaaac tttcagaaaa ggtttgttag
     1081 taaaatgaaa gctatacgta aggagtgttg tttgatgaaa ttttttcaag agtttttcat
     1141 tattattttc ttttctgtat taggagaagg catacggatt tttgctcatt tacctattcc
     1201 tggaagtatc ttaggtattc tttttttatt tttagcattt gaaatgcatc ttgttgatcc
     1261 tgaaaaaatc ggagcaactg gtgatttttt attgaataat ctagccattt tatttgttcc
     1321 tgctggtgta ggtttattgg aatattttga tgatattgct tcgatatggc ctgttttact
     1381 gggagcagtc gtcgtgtgtt ccatcgtcac gatggttgca gcgggaaaaa cggcagaagg
     1441 tgtagaagct ttgctgggct atgtccgtaa gaaaaaagca gaaagggtat ctgtaaacga
     1501 aatggaggag aatcaaattg actgagtttt ttgtgaatcc attatttggc ttaagtttga
     1561 ctttaggagt gtacgtatta tctgacaaat ttttgcggaa atataaactg cctgttttga
     1621 atcctctcgt attatcgatt gttgttatta tcttattttt gcatttttct gggatcagct
     1681 ataaagacta taatatcggg ggcaattttt tgactatgct gattactcct gcgacagtgg
     1741 cacttgccat ccctctttat aaaaacttcc gtttactgaa agagaatttt tatcctgtaa
     1801 tggctgcgat tttagcagga atagtcgcaa attgcttggt tagtgtaggg ttcggttact
     1861 tcttctctct tcataaagag atggtgatct cattactacc taaatctgta acgacggcta
     1921 tttctgtcga tttatctcat acgatgggcg gggtgaatgc cgtgacactt gctattgttg
     1981 tctctacagg tatttttggt tcgctgatcg ccactcatat tttccgccta ttcaagatcg
     2041 aaagtccagt tgctcgcggt gtggcgttag gaagtacttc tcatgctatt ggtacagcaa
     2101 aagcaatcga gattggtgaa atagaaggaa tcatttccgg acttgcgata tgcgtcaacg
     2161 gaatattgac cgttttattg ctaccgttat tctttcagcc ttttgctggc ttgttttgat
     2221 gcgaaaacca ctattttatg ctacaataac caaaacgttc gagcataaaa aaggagggaa
     2281 agacgtgtat ttgtcttccg agtatcttaa acaatttgat aaagatctat attataagat
     2341 tctcctccta gaaagttttg aggatcaagc ttggcatacc gctgcacaat tagcgcaagt
     2401 tgttcagctg gatgcccgca gcgtgtcgaa atacctgaac gagctgtcta aaaactatca
     2461 gcagtttagc ggtaagacac accctttgtt tacaaaaaat caccgttctg gttataactt
     2521 ttatgatacg ctcgattcaa ttgaacatga gcgttttttg atctatctcg tgcaaagtac
     2581 gctgaaattc cagttgctgc acgacatttt ttttgaagaa ttccatacaa tgtaccaatt
     2641 tgcgcaaaag cattacatta gtgaatctac tgctcatcga aaaatcaatg aatggaaaca
     2701 gcaactgcaa acttacggaa ttcggctgca acgaggtaca tatatcgctc aaggcgaaga
     2761 agaaatcatt cgactttact tgcatatgac attctggcag ttgtttcgag ggaaaatctg
     2821 gccatttgaa acgatttctc aaatggacgt aaagaatatg gcagaacata tcatggcttt
     2881 ttttaatgtt cgtctgaacg agatcaaaaa aagacgattg gaatatatgc taggtgcctt
     2941 ttttttaaga aaatcacaga agcattatgt tgtattgaat gagaaaaaaa gacggctgat
     3001 ttctgacaat ctgttgttcc agcgattttg ccaggtcatg gagccggtat ttcctaatta
     3061 ttttcaagta gaagacgagt taggcgcatt atttcttgta ctgatgactc gtgaagaata
     3121 ttatagtgat cctaagatac ggaagaaaat ttttgatttt catcaacaag caaaaacacc
     3181 gccctttaca gccctttcag aagcaaaagc cgctctttct ctttatcagg aagagcaggg
     3241 gctgccagca gaaaatctaa ccttcgaggc agaaaactat ttattcagca gtcatttttt
     3301 cgcctatctt tttcctaatg cgaaagaaac gattgatggc aatagtagtg attttattaa
     3361 tcatttagtt attgaaaaca aggaattgaa acaatggctc gtacattttt ttgaaagtag
     3421 acacaaacat cccaatcatc tggcatttaa aaatcatgcg tttttgatgg ggcggtatct
     3481 gactgtgttt aaaacattag gagcctttac tccacagctt ccaaagatca cgatcctttt
     3541 aatgacagat ttcccattat ttgaagagca actgttagag gagggattac ggacattttt
     3601 ccgtaatgag tatcagctga tttttctgcc aacagattat cgggggagag aagttgatct
     3661 tctgatttcg acaagtaaag tccaccgcaa accatgggcg gatcttgact attttattgt
     3721 gactgaagaa ctaaaattaa ttgattacat ccaactatct caaaaatttg aaatgattca
     3781 aaaacaaaaa ctattaaaac aataacaaag aaggagaaaa aagatgacat ttgaagaaat
     3841 tttaccagaa ttgaaaaaag gtgcaaagat tattcgtaaa ggatggagtg gatttgaact
     3901 ttatgttgtt ctcgtatccg atgatgaatt tgatggctgt ccggtgacgc cgtacttatt
     3961 gatcaaaaca agtgacgaag gtttttccag cttcgctcct acagtttgtg atattttagc
     4021 agatgattgg gcgatcgtag aatgaaaaac cagtatccag atctagctaa acaggttgta
     4081 tttatcacag gagctgcttc tggaattggt gctgcgcaag cccgagcgtt tcttgaacaa
     4141 gatgcttttg tatttggatt agatcaaaag ttcggtaaaa tggaagaaat ttctttgcga
     4201 tttcctgaaa cttttgccta cgcactaggt gatgttcgac aaatgaaaga actccaacaa
     4261 gcggtatcga aatgccaaaa tcattttggt gaagttacga ttttactgaa tacagcgggg
     4321 atattggatg cctacaaacc cttgatggaa actgacgaat cgctgtggga tctgatttat
     4381 gagacgaatg tgaaaagcat gtatcagctg acaaaaatca tccttcctga tatgatccgc
     4441 caaaaaagcg gaacgatcat caatatggct tcgattgctg gcttgattgc tggtggaggt
     4501 gggatcgctt atacaagcgc gaaacatgcg attgtcggat ttaccaaaca actcgctctg
     4561 gattacgcag gacagggtat ccgagtaaaa ggaattgctc caggagccat ccaaacacca
     4621 atgaatgcgg ctgattttga agggaaaggt gagatggcag agtgggtggc aaatgaaaca
     4681 ccagtcaagc gatgggcaca gccggaagaa gtagctgagc tgacgttatt tcttgcctct
     4741 cctcaagcaa gctacataca aggaacgatc atcccaatcg acggcggatg gttgttaaaa
     4801 taaccttgtg tttttttcaa gcttctgtat gataaaacta aagagagaaa caacggttga
     4861 tgataaaaaa agtcgatgac tgttcccgac attcgatgcc gaacagccag ttcaaaaaat
     4921 tctttttaat aatcgttaat attcgaaact aagcggcttt tcacgacctc ttgatataaa
     4981 cgatattcaa aagcttgcaa ctgcggtaac aagtccagcc aaatgaaaaa tataaggagc
     5041 tatttttatt tgaccattct tattacttgt tgcagggcag ctttttcact accttctgcg
     5101 acacttcagt tggaattttc ttcccatcct ttggtcgcgg tcattaccaa gataaaatta
     5161 tcttgcaaag gagaaataga acatggaaaa aaataatacc cgttcagcca aacgctggac
     5221 aacgacagat gtgactcgga tggcgctagt cactggttta tatgtggcag tgacgctggt
     5281 tttgtctgtc tttagctttg gagtgattca gattcgtctc tcggagatgt tcaactatct
     5341 gtccttgtat aacaaaagat acatcgtcgc ggtaacagcg ggagtagctt tggctaatat
     5401 tgcttctcct cttggactga tagatgttgc agtaggaagc gtcagcacgc tgcttgtatt
     5461 gcttatcaac tataaaatca ctagtcgcat caaaaacatg aaaatcaaaa tggccgtgac
     5521 agcatgcgta tttgcttttt ccatgtttac agtagctggt cagctaacga ttttatatca
     5581 actacctttt ttcttgaatt ggtgggtgat tgctttagga gaattggcat caatgattgt
     5641 cggtgggata attatttatt ggatcggtaa aaaagtggat ttgtcaaaat aaagatctgt
     5701 ttaatttaaa atagggcagc atggaagaga ctgaatgttg agtcttcttc catgctgctt
     5761 ttttgattaa tttgtatgaa gaaatcgatt tagccgtttt ttcatcaaga taccgatata
     5821 agctgctcca gttgccttga ttgcttccgg cagtaaaaac ggcagtagtc cactggcaaa
     5881 tgcagttgta aaagtaagat caccacttat tttcagccag atcgttccgc agatcaatgc
     5941 agcaagagag cccattagat tggcaaagat tgcatataag taatgagcat ccgttctttc
     6001 gatcatccaa ccagttatcc acgtttgtaa gagaaatcca atcaaatatc cgccagttgg
     6061 cccaaataaa acagctatac ctccagtcat ccctgaaaat acaggaagac caatcaagcc
     6121 aagtaaaaga taaactccaa cagatatcaa tcctagtttt cttccgagaa aagttgctgt
     6181 taatccaata gcaaatgttt gccctgtcaa aggaaccaaa ccaaaaggaa tggtcatctg
     6241 agaaaggaca gaaataatcg cggcaaattc cgcagcaagt atcaattctt tcaatgttcg
     6301 gttcataacg ccctcttttc gttaacttaa tatttttatt tggttaacga aattgtaaaa
     6361 caaagtgatt actttgtaaa tagaaaatat aaaaaaagac cagctatcca atagatagcg
     6421 gtctttttca agaaagcttt tgaaattata agctttggtt gtagtattca acgacaagag
     6481 cttcgtcgat ttctggatat agttcgtcac gttctggtaa acgagtcaat gaaccttcca
     6541 atttttcagc gtcaaagctt acgaatgctg ggcgtccaac agttgcttct acagcttctt
     6601 ttactgttac gatgttttgt gatttttcac gaactgagat cacttgaccc acttctacgt
     6661 ggtatgaagg gatatcaacg cgtttgccat caactaaaat gtgaccatgg ttaactaatt
     6721 gacgtgcttg acgacgagtc gttgcaagtc ctaaacggta aacaacattg tctaaacgtt
     6781 gttctagtaa gatcatgaag ttaacaccat gtttaccttc tttgattttg ctagctttta
     6841 cgaacaagtt acggaattga cgttcgttca ttccgtacat gtgacgaagt ttttgttttt
     6901 cagttaattg catgccgtat tctgattgtt tgccacggct attaggtcca tgttgacctg
     6961 gtgcgtaagg gcgacgtgct aattcttttc ctgtgcctga caatgagatg cctaagcgac
     7021 gagaaatttt ccatgatggt cctgtataac gagacattaa aatttcctcc aatgaatata
     7081 agtttttgga gtaaaataat ccgttgaaaa attcataatc atatagtttg ttcttcaatc
     7141 ttcacctttg cagccgcggt tacacaatcg aacccgagac ggggcaacaa actagggatg
     7201 agttattatt tttctgctgc attattttac acaaattaca gtatatgata gatcatccac
     7261 gaatgtcaag ataattagta cataaaaacg ctttacaaac ctaggctgtc tttataaaat
     7321 gagagggtat gtgcaaatgg aaaaaagcga aggaagttta aggagaaaat caatgaatcc
     7381 ggtatcacca ttatttgaga aaatcgatgc ttcaattgaa aagaaggcga atctttttga
     7441 aaacagcttt tcacgttacg ctgttcgagc ggttctcgcc tgtttgtttt tgactttagg
     7501 aacggctgtg gcatttgcga tcgcgatgaa gggggaagat atcgcgcatg gtctaggaaa
     7561 gattttatac gcatttatgt tcagctgggg tctggtcatg attctataca tgaacgccga
     7621 attaggaaca tcgaatatgc tttatatgac cgtgggtgtt tttcggcaga gacttgcttt
     7681 aagtaaggca gccaagattc tatttgcctg tatttttttc aatatgattg gcggtatatt
     7741 atgtggattt ctaatttcta tgactacgcc atttcaagat ttaacgttgg acaactttat
     7801 gttagaatca atagcagtaa aactagcaaa accttcagca acgattttgg tggaagggat
     7861 gtttgcgaat atcgttgtca ataccgctgt actgatcagc atgcggatga aagaggatgt
     7921 cggaaaagtg atcacagtcg tattcatcat tttcattttt gcttttttag gatatgaaca
     7981 cgtgatagcg aattttcctg cctttacgct tacttacttt gcttctcacg gacagatgga
     8041 tggaatgaca gtcagcaatg tgttacataa tttgtttttt gctttggcag gaaattatat
     8101 tggtggcggt ttagtaatgg gactgggata tgcttggctg aatcagtcaa aatcatccta
     8161 tgtggactaa aaagtcgttc attaaagggg aagtgccatg gaagttttag tttttccaaa
     8221 aaatgaggaa aaagcgttac aaatggaaaa gtacatgaaa aatctttttt cctttgctgg
     8281 agtacctaag ccggaacgcg ctcttttaca acaaccgtta ctggatgaga gcaaaaattt
     8341 atctatttct gaagttttag cttttgttca ttattattat gaacaaatcg aaagagagta
     8401 tcagtatgta gcgatcgatt tagtccagaa aaacatcaaa cggcttacat ttgaagactt
     8461 agtcgtattg ttgccactag ttcagaaaaa agaatggtgg gacagcatcg actcttggag
     8521 aaaagtatac agcacatggt gtaaagccta cccagagcag cgtgaagaag tctttcaatt
     8581 gtttgaaaaa aaggaagatt tttggctacg tcgaatagca atcacgttgc aattaggtta
     8641 taaacagcaa acagacacag gactattgat ccgagcaata gacgaagatc gtcaaacaaa
     8701 ggaattcttt atccaaaaag caattggttg ggcattacgc gattatagta agaccgatgc
     8761 cgattgggta aaaagacagc tgacaacaga tttgagtaag ttagctgttc gagaggcaag
     8821 taagtattta taagaaagcg agacgacaag agatgagacg caaattattt gcttttgata
     8881 ttgatggaac gttgttagat tcaaacaaac aagctttaga tagcacaaga gaagcattgg
     8941 aaaaattacg caaagccggt catctggtca cgattgctac agggcggagc cgatttcatg
     9001 cgcaagagat cattcgcgat cttgatttta cgaactacat tttatgtaat ggagcggccg
     9061 gttttttaga ccatgaacaa gtatataaga atttactaga tcgcgaccag ttggatgcgt
     9121 tcgttcaaga agcgcatcat gagcagatcg atacagcttt tgtcagctta gacaatatca
     9181 agcgttcttc ttccaatgat gtaacggtga tggaagaagc gatgcattct ttcggtgctg
     9241 tcttgcctga attagatcag ttttttgcag aggaacaaga aatataccaa gcgttagcat
     9301 ttttcgatca aacatatgat gggaagttct cttcatatga taaaattcga tttgtccgct
     9361 ggcatgagaa cagtgtagat gtcgtaccta aaaatggttc taaagcagcg acaattctgc
     9421 acttagcaga acaagtaggc atccctcatg aagacatcat cagctttggc gatggacaaa
     9481 acgaccgtga aatgttgcgc atgtcaggaa ttgggatagc tatggggaat gctgtgccag
     9541 aagtacaagc agaagctaag atggtcactg atacaaacga tcaggatgga atttggaagg
     9601 cattgaaaga gttaagcgtg atttaaaaga ggttgtgaaa aagctgttct gcatcaagta
     9661 ataagcccag ccaaacaaaa atagctcctc ctatttttcg tttttctggg cttattaccg
     9721 cagatgcaag ctttcgaaca ccgtttaagg aagaggtcgt gacagaagtg gtcagtttca
     9781 agaaataaga agggatttac gaaaattgct tctcaaattt ttgtaaaatt acggcttatt
     9841 tctgaagaag tagatgaaac aacgtttatt cgggaaatgg gactgtgaca agatgtttgt
     9901 catagtccca tttttttatt ttatttttca aaactaagaa ataccaggaa agagttcctt
     9961 ttgtttgacc ttttctttct tattgaaaac aactataata tagagtgatg ttaaaagaga
    10021 agaggaattt agtgtgcggg tattgtttat tggtgatgta gtaggttcaa tgggacgaga
    10081 gatgattact gaatatcttc ctagattgaa gaagaagtac cgtccgcaag taacgattgt
    10141 aaatggtgag aatgccgctt ctggacgtgg aatcacagaa aaaatctata aaaaattttt
    10201 gcaagacggc gtagatgtcg tgacgatggg gaatcatacg tgggacaacc gcggaatctt
    10261 tgagtttgta aatgaagcta aaaagatggt tcgtccggca aattttccgg aagggacgcc
    10321 tggacaaggg atggtctttg tcaaagtgaa ccaagtcgaa ctggcagtga tcaacatgca
    10381 ggctcgttct tttatggtgg acttggatga tccttttcgt aaaatgaatg agctggtaga
    10441 agaagccaga aaacgtacgc cgatcatttt tgtagatttt catggagaaa caacaagtga
    10501 aaaacaagca atgggctggt ttttagatgg gaaagtttca gcagtggtcg gtacacatac
    10561 acacgttcag acaaatgatg cgcgaatttt gccaagagga acagcgtatc tgacagacgt
    10621 tgggatgaca ggtccctatg atggtatttt gggtatgcgc agagaacctg taatcgaaaa
    10681 gtttctgact gcattaccaa aacgttttga agttgtggaa caaggccgat cgattttaag
    10741 cggctgtatc ttggaattgg atgatacgac aggtcatgca aaagaaatcc agctgattca
    10801 gatcaacgat gatcgaccgt tcatggagta gaaagagagg cagttttttg cagcaggagc
    10861 cggaaattca aaaagaagta aggaaactga caaaactttt aagagagaat gaaacgatca
    10921 ttcgctacaa agaacttgaa gaaaagatcc agcaaaacca gtatttagct gagttgagag
    10981 aaaaaatcaa acaagcgcaa aaagatgctg ttcattttgc ccactatgac aaaccagcag
    11041 cagaaaaaga agcaatcaaa caagcggatc aattcatgca agaatttgat cagcatccgc
    11101 ttgttgtagc ttatagaaaa cagctgttgg aagcagatga tctgcttcat catttaacaa
    11161 ctatgataca agaagaaatc aatggacaaa tagaggagga aaaacatgcc tcaaaaaact
    11221 aaaaatactc ctatgatgga acaatatcta agcatcaagg cccaatacca agatgctttt
    11281 ttgttctatc gtttaggaga cttttatgaa ctattttacg aagatgcgat caaagcgtca
    11341 cagcttttgg aattaacgtt gacgagcaga aatcgaaatg cagaagaacc tattcctatg
    11401 tgcggtgtac ctcaccatgc agcacaagga tatatcgatg ccttgattga aaaaggctat
    11461 aaagtagcga tttgcgagca ggtagaagat ccaaagacga ctaaagggat ggtcaagcgc
    11521 gaagtgatcc agctagtcac tcctggtaca gtcatggaca gcaaaggatt gtcggcaaaa
    11581 gacaacaatt ttttaacagc tgtagttagt caaggaaatg aatttggctt tgcttatgcc
    11641 gatttaagta cgggagaatt gaagacggct cggttgcatg atgaagaagc tgttttaaat
    11701 gaagcctctg cattgcagac aaaagaactc gtattaggca gtgagatcac tgataccctt
    11761 cgtgaacagc taagcgggcg tttagggcta gtcttttctc aacaaaatga gatggaagaa
    11821 aacgcggaat ttagcttttt gacaagcgaa ctagtcgatc cgctggaaaa agaagcaacc
    11881 ggcaaactat tgacttattt atcggtgaca caaaagcgga gtttagctca tatccaaaaa
    11941 gcagtagaat accaaccaga tcattttttg aaaatggatt attattcaaa attcaaccta
    12001 gaattaagcc aatctattcg gactgggaaa aagcacggca ctcttctatg gttgttagat
    12061 gaaacaaaaa cagcaatggg tggccgttta ctaaaacaat ggctagaccg tccgcttatc
    12121 caggcaaaac aaattaaagc aagacaagaa atggtcgctt ctttgctaga cagctatttt
    12181 gaacgtatcg atttgcagtc agcattaacg aaagtctatg atctggaacg cctagcaggt
    12241 cgagtagctt tcggtaatgt caatgggcga gacttgatcc agctgaaaac ttctttagaa
    12301 caagtgccaa tgatcagagg gttgattgaa ggaatcgatc aaggtcaatg gcaaagtctg
    12361 ctttcagaaa tgcagccgat ggaccatctc gtccaattga tcgaccaagc tatccaagat
    12421 gatccgccgt tacaaataac ggaaggaaac atcatcaaag atggattcaa tgagcagctc
    12481 gatatgtacc gttctgctat gagaaacgga aagaaatggt tggcagaact tgaagcaaaa
    12541 gagcgtcaag aaacaggaat caagaactta aaaatcggat ttaaccgcgt tttcggttat
    12601 tatatagaaa ttacaaaagc aaatcttgga aatgcagaac tggagaagta tgaacgaaaa
    12661 caaactttag caaatgcaga gcgtttcatc acaccagaac taaaagaatt ggagacacaa
    12721 atcctcgaag cagaagaaaa atctgttgat ttagaatatc agttgttttt agctgtgaga
    12781 gaagaagtca aaaaggctat ccagccgcta caagtacttg caaaagcaat cagtgcagta
    12841 gatgtgctac aaagttttgc aacgatcagc gaacgctatc agtatgttcg gccagaactt
    12901 gtcagtgaca agcatcagct tctgatcaaa gacggacgac atcctgtagt agaaaaagta
    12961 ttaggacatc aggaatatat ccctaacagc gtagaaatgg cagaagacga aatgatcttg
    13021 ctgattacag gaccgaatat gtctggtaaa agtacgtata tgcgacagtt ggcattaact
    13081 gttctgatgg cgcaaatggg ctgtttcgtt ccggcaaaac aagcagtttt acctattttt
    13141 gatcggattt ttacccgaat tggtgcaagt gacgacttaa ttgctggaca aagtactttt
    13201 atggtagaga tgatggaagc caatcaagcc ctgcgttatg ctacgccaaa cagtttgatt
    13261 ttgtttgacg aactagggcg aggaacagca acctatgacg gaatggcgtt ggcccaagca
    13321 atcatcgaat atatccatcg tcatgtacaa gcaaaaacac tcttttctac tcattatcat
    13381 gaactgacgg tacttgagaa agaacttccg cagcttaaaa atgtccatgt tggagccgtt
    13441 gaaaaagacg gagaagttgt cttcttgcat aaaatgatgg atggacctgc ggataaaagc
    13501 tacggcatac atgtggcaaa gatcgcagga ttgccttcca atctactaga acgagcagca
    13561 gatatcctgc atacgctaga aaatggcgat aacggacacc atactgttcc agctgaagta
    13621 gaagagaaaa ctaaaacagc caaacaggag caaataaaac cacctgcaga aaaagtaaat
    13681 caagaccata tacaagaccg tgtaaaagaa gaaaaccaac agctttcttt attcagtgaa
    13741 ttatccgaaa atgaaacaga agtcattgaa tcactgaagc aaataaattt actagaaatg
    13801 acgccaatgg atgcactaaa cacattatat gaattacaaa aacaactgta ggaagaaggc
    13861 ggatatcgat ggccaaaatc caagaattat ctgaacgttt agctaatcag atcgctgcgg
    13921 gagaagtagt ggagcgacca gcttccgttg tgaaagaatt agtggaaaat gcaattgacg
    13981 ccggcagtac tcagattgat atcttagttg aagaagcggg actgcggacg atccaagtga
    14041 ttgataacgg agaaggcatt ttggcagacg acgtagagaa tgccttcaag cggcatgcga
    14101 caagtaagat ccataatcgc gacgatcttt ttcgcatacg aacgctgggc tttcgagggg
    14161 aagctttgcc aagtattgcc tctgtttctg aaatgactct ggaaacggcg aatcaggaag
    14221 aaaaacaagg cacctatctg tcgctgaaag gcggagaagt tattgagcat cgaccagcac
    14281 cactgcgtca aggaacgaag attaccgtca gcaatctgtt tttcaatacg ccagcccgct
    14341 tgaaatacgt gaaaacactg cacacagaat tagcgaacat cggggacatc gtcaatcgac
    14401 tggcattgag tcatccatcg attgcttttc gtttagtaca tgatggaaac aaaatgctgg
    14461 caactgctgg aaatggcgat ctgaaacaaa caattgcggg aatctacgga ttatctaccg
    14521 caaaaaaaat gctgaaaatc gaaggaaaag atctagattt tgagttattt ggctatgttt
    14581 cattgcctga ggtgactcgt gccagtagga attacttatc gacgatcatc aatggacgtt
    14641 ttatcaagaa cttttccttg aataaagcaa tcgttgcagg ttatgggtca aaattaatgg
    14701 tcggtcgttt ccctattgct gtattggaaa tcaaaatgga cccgctttta gtagatgtaa
    14761 atgttcatcc gacgaagcag gaagtccggc tgtcgaagga aaaagaacta acagagctaa
    14821 ttagtaacgc cattcaaaaa gcattgtctc aagaaaatct gattccaagt gcagcggata
    14881 atctacggtt taagaaaaaa gtacctgatc agcctaaaac agaacaaatc gaaatcgatt
    14941 tgtcctacgc ttttgaagaa gaaaaaccta aaaagccaag tagtttgaat tttgatcgac
    15001 agacaggtac ctttttcgta gaagaactaa agaacacatt tgttgacaga attgttgata
    15061 agtctgtgga aaatgttgaa aactattctg gtttttctga agaaaacacc acttatccag
    15121 aaaaaacagc gaaaacggtg gataagtttg gtgaaaatgt tgataacttt gtagaaaaaa
    15181 cagtggaaaa atctgaaaag aaagaatttc ccattgtgga taacatggag aaaacaaatg
    15241 aagaacaagc agataaatcc gtagaagagg atgaaaagat ttacgaggaa gaattgacac
    15301 tccatcctga atttgatttc actcaaggaa acgacaaaac attagaacaa gccatcaaga
    15361 aattagaaac ggaaaaacca gtacaacgtt ttcctcagtt ggaatatttt ggacagatgc
    15421 acggaacgta tctttttgcc caaagtagcg atgggttgta catcattgat cagcatgctg
    15481 cccaagagcg cattaagtat gaatatttcc gggaaaaaat tggggaagta tctgatgatc
    15541 tgcaagaatt gcttgttcca tttgtgttag actatccaaa cagtgatgct ttgaaattaa
    15601 aagagcaaaa acacttgcta gaagatgcag ggatttattt agaagaattt ggtcaaaaca
    15661 gctttattgt acgggcccat cctacttggt atcccaatgg agaagaagaa tcgattattc
    15721 gggagatgat cgatatgctg ctgacaactg gaagtgtgag tgtaaaaaaa ttccgtgaag
    15781 caacagcaat catgatgagt tgcaaacgat cgattaaagc caaccattat ttgaatgaag
    15841 cacaagcacg tgtgctgctg aaggatctgg cacgatgcga aaatcctttc aattgtcccc
    15901 atggccgtcc agtattgatt cactttacca attcggatat ggaaaggatg ttcaaacgaa
    15961 tccaagaccc gcattaagtc aaagagaaga gggaaaacat gaagattgta ttagcatccc
    16021 aatcaccaag aagaaaagag ttattggctc atatcgtttc agaattcgag attgaacctg
    16081 ctgatatcga tgaaacgcca tttccagacg aacagcccgt cgattatgtt cgtagaatgg
    16141 ctgaagaaaa agcacgaact gtctgggaac aagatgaaaa agaagaccat ttagtgattg
    16201 ccagtgacac gactgttgtt ctcaatcaag agatcatggg taaaccagaa gatctggctg
    16261 aggctgaatt catgctgaaa aaactaagcg gggaaacaca tactgtatat acagctgttg
    16321 ttttaaaaac aaaggaacga gaagaacgga tactagcaga agcacatgtc actttttatc
    16381 ctttgacaga cgaagagatc aaacgctact tggagacggg ggattatgcg gataaggcag
    16441 gggcatacgg catccaaaat gctgcgagtg tattcgtcaa agaaatttct ggagattatt
    16501 acagtatcgt aggatttccg attggtgctg tcaatcaggc actgaaaaca ttcatttaaa
    16561 caaaaaagcg gactatctga ttttcgattg aaaatccatg atagcccgtt ttttttattg
    16621 aaaagattac ttatctactc tgactaagat tttacttcag ctttaccgac tgcttcgccc
    16681 gggcgaacaa gtcctgattg gtcaagagaa agtcctgcaa ctttttcatt attggtcaaa
    16741 gcaacaataa tgtcagtttt cttgccagct tgttctattt ctggcaaatc catttcagca
    16801 atcagggtct ctggtgtgac aaggtatcct tctttcacga atacattgaa aggaacaccc
    16861 tttagttcaa cagtatctaa tcccatgtga acaagtactt ccagcccctc attagaaaga
    16921 ataccaatgg catgttttgt ttcaaagaca ctcgtcactt taccgtttac tggtgcatag
    16981 actttgccgt ttgacggttc gacagcataa ccgtcaccca tcattttttg tgagaaaact
    17041 gggtcattta cttcagttat ttccttgatg gtccctgcag cgactgcgaa taatgtttct
    17101 tttgttgcaa ctgccgtacc agttgctgaa acaggttcga aagactgtgg cgttgctgtt
    17161 gcgccatttg ctgtttgaag ttgtacatca gcagcatttt caggaacagc aggatcaatc
    17221 ttattcaaaa ttccctgacg tctaaagatg atcgtcaagc caaatggaat gatgacagca
    17281 atgatcattg cgatgatgaa aggtacccat gtttctgctt ggatagctag tattcctggc
    17341 aaaccgccta ctccgatcgc gttagctcga acacccatca agttagcaaa catgccagct
    17401 aatccagagc cgaccattgc agcgacgaaa ggatagacgt atttcaaatt gattccgaac
    17461 atagcaggtt cagttacgcc taagtaacaa gaaatcattg ctggaatcga gatttgttct
    17521 tcttttttat tcccacgatg taagaagatg atggctaaga ctgcagagcc ttgggcgata
    17581 tttgataaag caatcatcgg ccataaattc gttgatccga aatctgcaat cagctgcata
    17641 tcgatggcat tggtcatatg gtggagacca gtaatcacta aaggagcata caggaatccg
    17701 aatacggcac taaacaacca gctgatagaa gacgtcaatc cagtatttac gatatttgag
    17761 atccagcttc cgactgtcca gccgatagga cctaaaataa cgtgtgcagc taaaacagtt
    17821 ggtaacaatg aaaacaatgg aacaaaaatc atcgagatcg actgagggat atatttgcgg
    17881 aagaagattt ctagatacgc taacataaac cctgccagca tggctggaat aacttgcgct
    17941 tgataaccaa tcatctggac ttgagcaaaa ccaaaatccc aaaatggaat atctgctgcg
    18001 gctgtggaag ctacactata agcatttaat aattgcggag aaactaacgt aattcctaaa
    18061 acgattccta atatttgtgt agtacccatc ttttttgcaa tactccaagt gatacctact
    18121 ggaagaaaat ggaaaattgc ttcaccgggt aaccacaaaa aggcattgac accattccaa
    18181 aacttcgagt gttcaacgat tgttccacca agactttcaa actggatacc ttcaagtaca
    18241 tttctgaatc caaggatcaa tccaccgaca atgattgcag gaatcagtgg tgtaaagatt
    18301 tctgctaaaa cggcaatagc acgttgaacg ggatgaaggt tttgttttgc agctgctttt
    18361 ccttgttctt tggaaacccc ttcaactcca gatactgcaa cgaagtcgtt ataaaaagta
    18421 gaaacatcgt ttcctatgat gacttggaac tgtcctgcat tcgtgaacat cccttttacg
    18481 ctaggtatat cttcgatcgc tttttcgtcc gcttttcctg gatcattcag gacaaaacgc
    18541 atacgagtcg cacaatgact aactgcagca atattttctt ttccgccgat atcatgtaac
    18601 agcttctcgg catctgcttg atattttgcc atatcaataa acccctttct cttaacttgt
    18661 ctatgcaagt tgtttcttca tttactatac tttttttcgg agagaattgc aagcgtttta
    18721 ttaaaaagta cgagagaaaa tgatatcgtt ttaaaaagct taaaataagc ttttcttaaa
    18781 aataggaaca ttgaaataca aaaagagctg tagtatagtg aagaaatgat ggaagaacga
    18841 gtgagggaaa aaacatgaat aagttcaatg aaattttttt agatttggaa cagaaaattt
    18901 tagcgaaaga atatcctccg cattcgcttt tacccagtga gaaccaattg atcaaaattt
    18961 atggtgtttc acgagaaaca attagaaaag cgctgaatct tttgactacg gaaggttata
    19021 ttcaaaaaaa acaaggaaaa ggttcgatcg tactggatcg gaatcgcttc gactttccta
    19081 tttctggatt gactagctat aaagagctac aggagaccca acgtattcca agtgaaacga
    19141 tcatccatac gttagaagaa acagaagtga caaaagcatt gaacgagatc actggctggg
    19201 agatcggtgc gcctgtttgg catttgatac gcgaaagaaa aatcaatggc gaagtagtta
    19261 tattagacac cgattatcta ttaaaggaaa tcgttcctat tttaactcca gcacaagcac
    19321 aaggatcgat ctatgaatat tttgaaaatg agctttcttt gacgattgct tacgcgcaaa
    19381 aggagatcac agtagaagaa gtaacggaaa tcatgaaaac agcgatggat cttcacgatg
    19441 acacttatgc agtagtagtc agaagcctcg ttcatctgga agatacccgc tgtttcgagt
    19501 attctgagtc ggttcaccga ttagataaat tccgtttcgt tgagtttgcc cgacgccgaa
    19561 aagtgtaaat agttcaaaag aaagcaaatt gttggcatct gtccactttg gcgctatgat
    19621 aggatcacaa agggggcatt tttaatggat aaaccaataa gaccagaaaa agaagaactg
    19681 aaagaaaagt tatcacccat ggcttatgca gtcacacaag aaaatgcaac agaacgacca
    19741 tttagcggga aatatgatga tttttacgaa aaggggatct atgtagatat cgtcagcggt
    19801 gaaccgttat tcagctctgc agaaaaatat gatgcaggtt gtggctggcc atcattcagc
    19861 aaaccaataa cgaaaagaca agtgcgcgaa aaagccgatt tttctcatgg catgcatcga
    19921 atagaagttc gaagcaaaga agcagattct catctcggac atgtgtttac cgatggacca
    19981 gtagatcaag gtggtctacg ttactgcatc aattctgcct cactgaaatt cattccatat
    20041 gaaaagatgg atgaattagg ttatggggaa ttcaaatcac ttgtagaata atggaaatac
    20101 acaaagagcg gtacaatacg gggatttact cgatcgtccg ctcttttttt attcaagcga
    20161 ttgctatttg aaagaaacgc tccctagact ccttttttca caaggtgaaa tgtgctacaa
    20221 taaagggaac gtatgtgtgc aaaagaaaga ggactagaat atgtatgaat atctgacagg
    20281 tgtggttacc tttatcaacc cctattacat agtagtagaa accaatggta tcggctacca
    20341 gatcgcacta ggaaatcctt accgctacag tagcaaacta aatcaaaaaa taaaaattta
    20401 tgtccatcag gtaatacgtg aagatgcgca tcttctgtat ggatttgatt cgctagaaga
    20461 aaagcaactg tttttgcgat tagtaagtgt ttcaggaatc ggtccgaaaa gtgcccttgc
    20521 aatcatggca agcgaagacc actccggttt gattcaagca gtagaatcca gtgatgtcac
    20581 ttatttgacg aaatttccag gagtagggaa aaagacagca caacaaatga tcttggatct
    20641 aaaaggaaaa ttcggtgagc tgagtatcga tacacctttt agtctatttg atgaagcaaa
    20701 cacaaaagac gctacagctc tatctgaagc aatggaagct ctctcggctc ttggttacag
    20761 tgatcgagaa atcaaacgtg tagaaaaaca attaaaagaa gtagataatc agacaacgga
    20821 tgagtatcta cgccaagcac tcaagctaat gatgaaaaaa taaggaggag accaaatgac
    20881 agatgaatac ttgttatcac cagaatctgg cgaaaatgaa ctgagtttgg aaaaatctct
    20941 tcgaccacag ctgctttctc aatatatcgg acagagaaaa gtcaaacaag aacttcagat
    21001 ttacatcgaa gctgccagaa atcgggaaga acctttagat catgttctgt tgtacgggcc
    21061 tcctggatta ggaaaaacga cgatggccat ggtcattgct aatgagatga ccgtaaatat
    21121 tcgtacgacg agtggccctg ctatcgaacg tccgggtgac ttagtagcaa tcttgaatga
    21181 actggaagca ggggatgtcc tgtttatcga tgagatccat cgattaccta gagtggtaga
    21241 agagatgctt tattcagcta tggaagattt ctatgtagat atcatggtag gacaagggcc
    21301 aaccgcacat ccggttcatt tccctcttcc tccctttact ttgattggcg cgacgacacg
    21361 ggcaggtatg ctttctgcac ctttacgaga tcgatttggc atcatttctc atatggaata
    21421 ttatgacgaa caggatctgc aggagatcgt tttacgttct tcggatattt ttcaaacaga
    21481 gatcattgaa gaaggggcat tggaaattgc tcgccgatca cgaggaacgc ctagaatcgc
    21541 taatcgcttg ctcaaacggg ttcgtgattt tgcgcaagtc caaggagacg ggaaaatcga
    21601 tcgtcctata gcggatcagg cacttacttt gctacaggtc gatcaggctg gattggatta
    21661 tgttgaccag aaattgttgc gaacaatgat cgagttatat ggcggcggac cagtagggtt
    21721 aagtacgctt tcagtaaaca taggagaaga gcgtgagacg gtagaagata tgtatgaacc
    21781 gtatctgatc caaaaaggat ttttaaagcg tacacctaga gggcgaattg ctactgctta
    21841 tgcctatgaa cattttggtt acgactatca tatatgattt ttgcttttag attggaggaa
    21901 aatgaatgag tgatacaaca cgtacgaaac aattgatcaa aaacgtattg attgatttgt
    21961 gccaaacgca atcttttcga aaaatcagcg tccaaaaaat cgcaaacgaa gcaaaaatca
    22021 atcgacaaac attttattat cattttacag ataaatacga tttgcttcgt tgggtctatt
    22081 gtgaggatgc attgcattat ttagatccac aatgtctttc acttgataat tgggaagagc
    22141 aagcattgaa aatgctgaaa gcaattgctg agaagaaaga attttatgca aatactgtgc
    22201 tttctgaccc agaaatttta caaaaagagt tttctcaact gatccaaaaa tcatttatca
    22261 agatttttga acaaatggat gaagaaagac aactttctgc aagagacaaa ctattttatg
    22321 ctcgtttttt ttcttacggt tgcagtggtg tattagtcaa ttggatcacg aatgaatatg
    22381 aagaaacacc gatggaaatc gctatgcagc ttttccgttt agccaaggat acggaattat
    22441 attcttatcg tctttatgca aaagaagagt tggaagaaca gtaatagaaa aattttttgt
    22501 tggaaaagca ataatcgcta aaaggagatg gggaaatgac aaaaatttta tttgtttgtt
    22561 tgggaaatat ttgccgttca ccgatggcag aaggcttgct gagaaaacga atagcattag
    22621 aaggaaggga agcagaattt tttgtggatt ctgctgctac aagcacgtat gaagttggaa
    22681 agacaccgca ccctggaacg aaaaaaatat taaaccaaga aaaagtagat atgacgaata
    22741 tggttgctcg gcaaatcaca ccccacgatt ttgaaacttt tgattggatc attggcatgg
    22801 atcaagaaaa tgtagaagaa ctgaaacgaa gagcaccacg ctcagcgcag gggaaaatcc
    22861 atttgttttt aagtagcatt cctggaaaag aaaaagaaaa tgttcccgac ccatactata
    22921 ccaataactt tgatgagaca tatcacttga tcacagaagg cctgaatcat tggtggaata
    22981 tttggattga tgaaaataaa ataacaaaca atttgtaaag ttaacgcttt ctttttaagg
    23041 cgatgttaga gcatttcctt gtttttttgt aaaatgttag gtaaaataaa attaattaag
    23101 taacactttc ttagctaaag gaggagtgca ttcgatgaac gaagagacag tatcgccaat
    23161 gaagaagctt acgaagttag ttactcatac ttcgtatcaa ctttttgatt cttttgtaaa
    23221 tgacactgaa gaacatgcta agaatgtacg attgcaagga aagtttattc ttgagctgca
    23281 aaaagctgct actgaacata gtctagttat cttacaagta agagcaaatc gtttgagtag
    23341 caagtatgaa acgatctcag gctgggtggc gacaaaaacg gtcgaaaaag accgagtgat
    23401 gatccgccta caaaacgaag agcagcagat ccggatggtt cctgtagaac aaattgaaaa
    23461 aataacaatc ctttcaccag caggggaaca ggaacggtta tcaaaataat ttataccaga
    23521 tagaaaagca cgtatgagct tgtaccatca atcattacta atcaaaaata accgaatgct
    23581 ttaagctgat atcagtaaaa cggaacgttt taggatgagt atcatagcat gacagcattc
    23641 ggttgattag ttgaatcgat tggatggtac aagttttttt gataaaaaaa ggtcctgcta
    23701 gccggggagc tagcaggata aggagttaaa aatgaaaaag tgttgttagg gttgtttgtt
    23761 ggtatgctta tataatagtc attagttgtg aatattttgt gacgaaagtt ttcgaaagtt
    23821 gttaagtttt
//
LOCUS       DANKKG010000031        23743 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000031 DANKKG010000000
VERSION     DANKKG010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23743
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.031"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            12..644
                     /locus_tag="PW004_001044"
     CDS             12..644
                     /locus_tag="PW004_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726032.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(778..903)
                     /locus_tag="PW004_001045"
     CDS             complement(778..903)
                     /locus_tag="PW004_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2726033.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(1037..1930)
                     /locus_tag="PW004_001046"
     CDS             complement(1037..1930)
                     /locus_tag="PW004_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HDL2726034.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEIAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(2112..3038)
                     /locus_tag="PW004_001047"
     CDS             complement(2112..3038)
                     /locus_tag="PW004_001047"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HDL2726035.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(3123..3884)
                     /gene="pflA"
                     /locus_tag="PW004_001048"
     CDS             complement(3123..3884)
                     /gene="pflA"
                     /locus_tag="PW004_001048"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HDL2726036.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(4128..6359)
                     /gene="pflB"
                     /locus_tag="PW004_001049"
     CDS             complement(4128..6359)
                     /gene="pflB"
                     /locus_tag="PW004_001049"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDL2726037.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(6636..9086)
                     /gene="parC"
                     /locus_tag="PW004_001050"
     CDS             complement(6636..9086)
                     /gene="parC"
                     /locus_tag="PW004_001050"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HDL2726038.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(9098..11143)
                     /gene="parE"
                     /locus_tag="PW004_001051"
     CDS             complement(9098..11143)
                     /gene="parE"
                     /locus_tag="PW004_001051"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HDL2726039.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(11309..11743)
                     /locus_tag="PW004_001052"
     CDS             complement(11309..11743)
                     /locus_tag="PW004_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HDL2726040.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            11870..12508
                     /gene="plsY"
                     /locus_tag="PW004_001053"
     CDS             11870..12508
                     /gene="plsY"
                     /locus_tag="PW004_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HDL2726041.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(12640..13515)
                     /locus_tag="PW004_001054"
     CDS             complement(12640..13515)
                     /locus_tag="PW004_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HDL2726042.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(13597..14388)
                     /gene="codY"
                     /locus_tag="PW004_001055"
     CDS             complement(13597..14388)
                     /gene="codY"
                     /locus_tag="PW004_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HDL2726043.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(14406..15806)
                     /gene="hslU"
                     /locus_tag="PW004_001056"
     CDS             complement(14406..15806)
                     /gene="hslU"
                     /locus_tag="PW004_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HDL2726044.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(15820..16368)
                     /gene="hslV"
                     /locus_tag="PW004_001057"
     CDS             complement(15820..16368)
                     /gene="hslV"
                     /locus_tag="PW004_001057"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HDL2726045.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(16405..17310)
                     /gene="xerC"
                     /locus_tag="PW004_001058"
     CDS             complement(16405..17310)
                     /gene="xerC"
                     /locus_tag="PW004_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HDL2726046.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(17508..18884)
                     /gene="trmFO"
                     /locus_tag="PW004_001059"
     CDS             complement(17508..18884)
                     /gene="trmFO"
                     /locus_tag="PW004_001059"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HDL2726047.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(18853..20931)
                     /gene="topA"
                     /locus_tag="PW004_001060"
     CDS             complement(18853..20931)
                     /gene="topA"
                     /locus_tag="PW004_001060"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HDL2726048.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(21053..21910)
                     /gene="dprA"
                     /locus_tag="PW004_001061"
     CDS             complement(21053..21910)
                     /gene="dprA"
                     /locus_tag="PW004_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDL2726049.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(21966..22733)
                     /locus_tag="PW004_001062"
     CDS             complement(21966..22733)
                     /locus_tag="PW004_001062"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HDL2726050.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(22726..23586)
                     /gene="ylqF"
                     /locus_tag="PW004_001063"
     CDS             complement(22726..23586)
                     /gene="ylqF"
                     /locus_tag="PW004_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HDL2726051.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
BASE COUNT         6177 a         5124 c         4081 g         8361 t
ORIGIN      
        1 ttggagatat tatggaaatt caacgtagaa ccactaattt tttacattta ggctcttttt
       61 ttgcaacttt gacgatctta ttttttctat gttattcata ccttaagacc tcacatgcgc
      121 cttctagtgc attaggctgg ttgcttttgg atcattctga tatcacagaa gccgaagaaa
      181 atccgttttt tttgattttg tcttctcttt gcggcttact gttttctgtt tatttcggtg
      241 cctatttttt tcatcgacat cctggtacgt taaacgaagc ttcaagcgta ttgattgcat
      301 ttaaaacgtt ttctttttta gcaattcttt tttattcgtt taatcacaat acgcttttct
      361 tctttatcat gaatggttta gtcgtgattt tatccatttt tacttatttt attttttcac
      421 tcatttttag ccaattgaaa tgtccgctct tctttcgatt agttcctgtc acctgtttta
      481 tttacagctt gttttccttt tttgtccttg ctttaatgcc actgaccaac ccttcgacga
      541 tccaagtcgc aggaaatctg ttggatatgc tcttcatcgt cagtctaggt gtatttatgt
      601 ggcaaagaaa aaacaaacaa aataaaaagc attttgaagc ctgactatta cgacttcata
      661 aatgcttttt tagttttaaa cacttcaatc taaaagaata acccaagatt ggataaatta
      721 ttaatgactc gccattcgtt tcttagtgat tctggattat caaatggact tcctaatcta
      781 atcagctagt tcttcccata aaatgccata cttgactgaa tcataatatt gattatttaa
      841 aaatctaact tttcttatca ctacttcctt tgtcattcca catttctcac catttttttg
      901 cattcctttg tttccagacc atgtagtgaa tctaataggt ggtagatagg caaaatactc
      961 aaataattct tttaaccata aaggaagaga aaggctcctc ctgcaagagt cagtataatt
     1021 tttgcttact gctctcttag gttttactca ctaaaactgt tccatattct ttgatataag
     1081 aaaatgcatg cggtataaat ttcttgctca gctcagaaaa gatttgtcgt ttaagaaagg
     1141 ctatttcctc cattgtcaat gaaggatcca atgttggtaa ttcaatctca aaattgtttt
     1201 tttcgacaat aggtaattct aatgtgaatg attcttcttc ctttatagta ttcgtcacaa
     1261 gtagagactg atggaaaata ctaggccggc gatttttata tttattctta cgtatcctgt
     1321 caacgataac ttctatttga tcaaaaaagt acgattcatt tacgtctcca ataagttcgg
     1381 ctaattcttt gaatgccttc gttacaggaa gctgattctt ctgtacaaaa aagtaattag
     1441 ccaaagtaag cgggaagtgt tctatagagg aaagtgtgat aaaacggtac ttctcatttc
     1501 ctgcagttgt cctcagcaat tctgtctctc ggctgtttct tactccataa aaatacgctt
     1561 ctttccgttc atcttcgaaa caatacgccc aaataacttc tcccatcgct aatttttgtt
     1621 catcaacaga gagtctcttc aattgttcag ctatggtttc agcagccgaa gtggcttgtt
     1681 gcaagtaatc tttaggatca aatattggaa agttcaaatg atatcttcgt tcttccctct
     1741 tgattaatcc tgcatcgatc aataaatcaa gaaaatggga aaatttattt tgcgaaaatt
     1801 cttttttcaa ttctcttaga atcgtttctt gttctcgatg ttcatataga taatcggtca
     1861 atgaagaaaa aagcggatca tcggtcagtt tttgataaaa gttttcctta ttttttgaat
     1921 aatagttcat atatgttctc ttttctatcc gttttatttt acttaaaagg cttttacgta
     1981 aaaaggagga agaacaagag tcataattga ctgttattct cctccatttc ttcggccttt
     2041 agtatgttct atgtttcttt ttatatcaat cgcttatcca ttcattttga atgcttcata
     2101 ctaccatttg attagctaaa tacagccgtc aattgaggta cgacttgttt tttacgtgaa
     2161 acgacacctt taagcaaagc acggttgttg gtcaaagttg tttggaaagc ttcttctact
     2221 ttttcttttc ctttaccagc aacaagtaat tctgaatcac tgtctaagat gtttgtgaca
     2281 actaatacga acagatcgta gccatttgct ttcatttcat cttccattgc tgcttctagc
     2341 tctgcttgtc gatccatcac ttcattaagg tccactgtat tgacttgagc gatacgaacg
     2401 ttggaactgc ccattgggaa actttttgca tccaaatcaa gaagaaccgc agctgattta
     2461 tcacttagat ttgttcctgc tttcaacatt tcaagtccat aagtgtttag atcgacttca
     2521 gcgattgctg caagttcttt tgcagctgct tcatcttcag gcgtacatgt tggtgatttg
     2581 aacaacaatg tatctgaaat gatagctgag agcatgattc ctgcgatttc tttaggaatt
     2641 gttacttggt tttctttata tagttttaaa ataatcgtac ttgtacatcc aactggttct
     2701 gcacgatagt acaatggatt agctgtttca aagtttgcaa tacgatggtg gtctactaca
     2761 gctaaaatat ttacttcgct gatatctgaa atactttgtt ggaattcatt atgatccaca
     2821 agcatgacat ctgcattttc tgctttagct gactctacta ctcgtggtgc agatagctga
     2881 aagtggtcta atgcgtattg tgtttcttca ttaggtgtac ctagagcaac tgcttctgtt
     2941 tctttaccta attcattttg caagtgtgaa aacgcaatgg ctgccccgat tgcatctgta
     3001 tctggatttt ggtgaccaaa aactaaaatt tttgacatgt cattcgctcc tttgtgattt
     3061 ctcctattta ataatagcaa aaaaacggaa agaagcaata cttatcttct ttccgatgcg
     3121 acctatcgcg ttaaatattt tgtatactct tctgtatgaa gcaatcgttt agcattttcg
     3181 acacgttctt ttgtaggtgg ttcgatccct tctaatggat atttcaaacc aagtgtttcc
     3241 cacttgtatt ttcccatcgt atgataagga agaatctcta ctctgtctac atttgacaag
     3301 gtttgaataa agttgtctag tcggatcaaa tattcatcat aatcacttcg ctcaggaact
     3361 agtacatggc gaatccaaac cggtttgttg atttttgaca aatattgagc catttctaaa
     3421 atattttcat tgtcccaatg cgttagtttt ttatgctctt cattatcgat gtgtttgatg
     3481 tcaaataaca gtaaatctgt atacttcatc aattcttgaa aacgtgaaaa gaaaggttct
     3541 tcatacgtaa aaggttttcc gcatgtatcc aatgtagtat gcatcttccg ctcttttgct
     3601 tttttaaata gttcgatcaa aaaatcgatc tgcaatagag gttctccacc actaacagta
     3661 ataccacctt tatctcccca gtaaggtctg aagcgttctg ctttatctaa aagttcatct
     3721 gctgtgtatt ctttccctcc gccaatattc caagtatctg gattgtgaca aaattggcaa
     3781 cgcatgcggc atccttgcat aaacacgaca aagcgcagtc caggtccgtc tactgaaccg
     3841 aaagtttcga tagaatgaac atagccgatt gttttttctt ccatttaagt tcacatcttt
     3901 tctttgattt cattttaagt acaagttagc tgtcgaagcg aagctttctg aacaaaattt
     3961 ttcgcaattg ccgcttcgtt atgtgtaaaa tttacaggga cggaacatac aagtcctgcc
     4021 cctgtaaatc gtttctttca aataagcata ctgattattc gattttgatg cgatggctat
     4081 tcaacgtcct ttcgacgcta agtcgaaaaa cttttttcac gaacttttta cattctgtcg
     4141 tgtgaagttc tcatcaagac atctaattgt tgttctcttg tcaagtcacg gaatttaact
     4201 gcataaccag atacccggat cgtcaagttc ggatatttca ttggattttc cattgcatca
     4261 cgcaataaat cattactgaa tacattgaca ttcaaatgat aagctccttt tgagaagtat
     4321 ccatctaaga cattacgcaa gttttcgatt cttgtatctt gttcttttcc aagacctgaa
     4381 ggattgattg tttgggtatt tgaaattcca tccaatgcac agccgtaatt cattttagct
     4441 gttgagttca ggctagctaa taacccactt tgttccgcat tgtaacttgg atttgctcct
     4501 ggcgcaagtg gttttccagc ttcacggcca tcaggagtat ttcctgtatt tttaccgtaa
     4561 acaacatttg aagtaatcgt caataatgaa gttgttggtt ttgagttgcg gtaagtttta
     4621 tgacgtttga tttgtcccat gaagtattcc aagatccatg aaactaattc atctgcacgg
     4681 tcatcatcat ttccgtattt agggaagtcg ccatccactt caaaatcaac tgctattcct
     4741 ttttcattac ggattgcttt tacttttgca tatttgatcg ctgacaagct gtctgccgca
     4801 tgagagatcc cagcaatacc tgtagcaaat gtgcgttgca agtcagaatc catcaaagcc
     4861 agctgagctg cttcataaga atatttatca tgcatataat gaatgatgtt caacgtattg
     4921 acatatagtt ctgctaacca atccatcata ttcatgaagt tatgtttcac ttcttcaaag
     4981 tctagtattt ctgatgtgat tggacgatat tctggtccaa cttgggcgcc agtcacttca
     5041 tcgactccgc cattgatggc atataatagt gttttagcca agttagcacg agcgccaaag
     5101 aattgcatgt cttttccgac aactgttcct gatacacagc aagcaatcgc acaatcatca
     5161 gaaccccatt gttcacgaag ctgattatca ttttcgaatt gaatcgaaga actttcgata
     5221 gcgattctgc tagcatatgt tctgaatcct tctggaaggt gttcagaata caaaacagtc
     5281 aagtttggtt ctggtgaagg tcccatattt gttaatgtat gaagcagacg gaaatcattc
     5341 tttgtaacta atgaacgacc atctaatccc atacctgcaa tggataaagt agcccagata
     5401 gggaatcctg agaataattg gttatattca ggtgtacgcg cgaatttgac catacgtaat
     5461 ttcattacta agtggtcgat caattcttgt gcttcttttt ctgtgattac tccattatcc
     5521 aaatcacgtt ggatatagat atcaaggaat gctgagatac gtccgattga catcgcagca
     5581 ccattttgtg atttgattgc tgctaaatat ccgaagtata gccattggac agcttctttt
     5641 gcatttgcag ctggtttaga aatatcatag ccatatttcg cagccatttc tttcatttgc
     5701 gctaatgcac gatattgttc agtgatttct tcacgtaaac gaatcacttc ttcactcatt
     5761 gtatgataac cagtcatatc atgatctttt ttcttttgtt ccatcaaata gtcgattcca
     5821 tacaatgcca cacgacgata gtcaccgatg atacgtccgc gaccgtaagc gtctggaagc
     5881 cctgtaatga ttttgttttt acgagcagca cgcatttcag cagtataagc gtcaaataca
     5941 ccttgattat gtgttttgcg ccagtcagtg aaaatatgtg tcaattcatc gtttggtgta
     6001 tagccattgc tttttagcgc attgttagcc atattgattc cgccgaacgg catgaatgct
     6061 tgtttcaaag gtttgtctgt ctgtagaccg acgatttttt ccaaatcttt atccaaatag
     6121 cctgcatcgt gtgaagtaat agttgatacg atttctgtgt ccatatcata gacaccgtta
     6181 cgctcgaatt ggatatcatt taacttttgc aattcatccc ataatttttc tgttgcttct
     6241 gttgggcctt ccaaaaagct gtcatcaccg cgatattcag tgtagttatt ttgaataaag
     6301 tctctagtgt ctacgccttc tttccatttg gtacctttaa atccattcca ttgctccatt
     6361 gtatggacct cctaaatttc tgttttatgt aacagttttt acgtgattag tataacacag
     6421 caccctatct ttgcaagggt tttcttgttt tttatttaac aatttttatg ttttatttac
     6481 atatatttaa atcaacgttg acataccaac atttaagcat tgtgaacact ttcatctttt
     6541 tttgcaaacg tataaggatt tattaaaaat gaatgaaaaa gagacgccac ctgtattaat
     6601 acagatggcg tctcttcatt aaaatactac aacagttacg actcgatgaa aatctcagga
     6661 gcatcgataa cttcgatgac ttctccacct tttttctcat ccatcgcaaa tgatccattc
     6721 gatgttcgat cacctatagg gaaatcagtt gggtcaatcg ttaactgctg ccctttttga
     6781 ttcatcaagg ttatttcttt tgatcttcct tccgacatat agataatacg gtgcggattt
     6841 tttttcagct cacgcatgac cgtcactccc cgcttcgcac gtgaagtttg gctgatttct
     6901 tgagcaagca tgcgtttaac agctcctcgc tgagtaacga ttacgatcgg cgtatcacct
     6961 tctgctaaca caagcagccc gttgataatc ctatctccat ctttcaaatt catagacttc
     7021 actccagctg cctttgctcc aacaacggga acatctgtta aaggatagcg caaacccatt
     7081 ccttgatgcg ttactaagaa aacatctaag cctggctgat cttctttcag atacacagca
     7141 atcagttcat cttcttccgc ctttagcttc atacagctga gcggacggct cttataggtg
     7201 cgccatggtt cgaactctgt cattcgtgtt tgcttgatca atccattctt gctgatgaat
     7261 acaaatgttt tctctgcttg aattttttga tatgggaaaa cagctaaaat cgattcgtct
     7321 acagacagat tggagattgt ttgagaaatg tgctcaccag catctttcca tttcaaatcg
     7381 ggtagttcat ggacagggcg gtagatcaca tttgctttat ttgtgatcag aagaacatgg
     7441 tccaatgtac tcaattcacc tgagtatagc aagtagtcgc cttctcgcat cccgatttct
     7501 tctggtttgg aagcactata agaacgaagg cttgttcgct tcacatagcc ttctcgcgtc
     7561 acactgacaa ttacgtcttc ttgtgccact aagacttgcg tatcaatttt gatttcttcg
     7621 atttcgtctt cgattgtcgt tagacgagcg gaagaataat tttttttcac ttctcgcaat
     7681 tcttttttca taacagagaa aagctctttg tcatttgaaa ggattttatt caactctgtg
     7741 atttgcgcaa tcaagctttc ggattccttt tgaagctctg tgatatccgt gttcgtcaaa
     7801 cggtataatt gcaatgtgac aattgcttct gcttgttctt ctgtaaattg aaagacgtcg
     7861 actaaattct ttttggcgtc ttttttgtct ttactttcac gaatcgttgc gattacttca
     7921 tccagaatcg ataaagcttt catcaatcct tctacgatat gctgtctttt ttgagctttg
     7981 gctaaatcga attgactacg ttttgttatg acttgctttc gatgctcgat ataacttctt
     8041 aaaatgtctt tcagtccaac ttgatgcgga gtcatatggt cgatagcgac catattgaaa
     8101 ttgtaattga tctgtagctc agtgtttttg aacaaataat tcaaaattcc ttgagcattg
     8161 gcgtcttttt tcaattcgac gacaatttgc agacctgttc tatcactttc atctcgtact
     8221 tcagctattc catcgatttt cttattcaaa cggatctcat ccattttttt caccaaggtc
     8281 gctttattta cttcatatgg gatttctgtg atcacgattt gctgtttatt acccttcatt
     8341 gcctctatct ttgttttcga acgaagaata acttttcctc gtcctgtttc ataagctttt
     8401 ttgatttcat cttttccttg caagatccct cctgttggaa agtcaggtcc aggaatatat
     8461 tccatcaatt tttcaaggct cgcttgtgga tgatcgatca tatagattgt gccatcgatc
     8521 acttcagcta agttatgagt cgggatttct gttgcatatc cagcagatat acctgtcgat
     8581 ccattgacga gaaggtttgg gtatcgagct ggaaggactg ttggttcttt ttctgtatca
     8641 tcaaaattcc acacgagatc gactgtttct ttctcgatat ctgctagcat ctctccactt
     8701 aacttggata atctggcttc agtataacgc atcgcagcag gtggatcacc gtccatactc
     8761 ccgttattcc cgtgcatttc gatcagtact tcccgcaact tccagtcttg gctcatgcgg
     8821 accatcgctt cataaatact gatgtcacca tgaggatgat aattccccat gatatttccg
     8881 acagattttg ctgatttacg gaatcctttg tcatacgtat tgccatcttt gttcatggca
     8941 aataatatcc ggcgttgcac tggcttcaat ccatcacgga tatcaggcag tgcacgttct
     9001 tggataatat atttggaata acgtccgaag cggtcaccca tcacttcttc taaagttaat
     9061 tcttgtactt cttgtcgatt ttccatcagg tgacctccta ttctacttca aataactgat
     9121 tgttttcatt tttatctgct cgttcttcat ctaataaatc attagaaact gaaggagaga
     9181 tttcagtatc ctctttttta tctaagatac tgccatcttc ttcgagcgta aattgcacgt
     9241 gattttcgat ccattttctt cgaggttcta ctttgtcacc catcagtgtg gttactcgac
     9301 gttcagcttg cgcagcatca tcgatacgga cgcggatcaa cgtacgggaa gtcggatcca
     9361 tcgttgtttc ccacaattgt tccgcattca tctctccaag tcctttatat cgctgcagca
     9421 tatatccttt gccgactttt ttaatcattg ctgccaattc gtcatctgtc cacgcatatt
     9481 caatgacttg cttctttcct tgtcctttgg atactttata taaaggcggt aacgcgatat
     9541 atactttccc agcttcaatc agcggtttca tataacgata aaagaaagta agcagtaata
     9601 cttgaatatg ggcgccgtct gtatccgcat cggtcatgat gattactttg tcgtaattac
     9661 agtcttcgat agaaaactca ggaccgactc ctgcaccaat cgtatagatc atcgtattga
     9721 tttcttcatt tttcaagata tcttgcattt tagctttttc ggtattgatg acttttcctc
     9781 gtaaaggtag aattgcttgg aactttctgt ctcttccttg tttagcagat ccgccagcag
     9841 aatccccttc gactaaatac aattcatttt tcttagggtt tcgggattgt gcgggtgtca
     9901 attttcctga aagaagagat tcgccttttt tacgcttctt cccatttcgg ctttcttctc
     9961 tggctttacg tgctgcttct ctcgcttctc tggctttgat tgctttacgg accaacatct
    10021 ggctcatttc gctattttcc tgcaaataaa agcccatttg ttcactgatg acgttatcta
    10081 ctacggtacg agcaactggc gtaccaagtt tttcttttgt ttgcccttca aattgcagaa
    10141 gattttcagg tacacggata gaaagtacag cagctaaccc ttctctgaaa tcactacctt
    10201 ccagattctt gtcccgttct ttcagcaaac caacttttct cgcatattca ttataagatt
    10261 tcgtcatcgc cgctttcata ccagcttcat gggttccccc atcttttgtc cgaacgttat
    10321 tgacaaaaga taaaacattt tctgaataac cgtcattgta ttgataagcg acctctactt
    10381 cgataccttc tttttctcct gaaaaataaa caactggtgt caatgtatct ttttcttcat
    10441 tcagataatc aacaaattct ttgattcctt cttcgtaatg aaaaatttcc ttgactggtt
    10501 cttctccacg aagatcagaa agagagattt taacgccttt tagaagaaag gcagattctc
    10561 ttaaccgttc ggctaaaatt tcatatgaaa atttcgttgt actgaatatt gtatcatctg
    10621 gtaaaaaatg gacagtcgtc ccgtttgctt tttttgtttt tcctatcttt ttcaaagtac
    10681 cgtcaggttt accgccgttt ttaaatttct gctgatactc tacgccatct ctaacgatcg
    10741 ttacaattag ccatttcgat aaggcattta ccacactggc tcctacaccg tgcagtccac
    10801 cagaagtttt gtaccctcct tgaccaaact ttccgccagc atgcagcaca gtaaaaatca
    10861 cttcaactgt tggtattccg gaagcatgca ttcctacagg catcccacgt ccgctatctg
    10921 caacagttat actattgtct tcatggatgg tcacatcaat ctcactgcca taacctgata
    10981 atgcttcgtc aactgcatta tcgacgattt catagactaa atgatgcaag cctcggctgt
    11041 cagtggatcc aatgtacatc cctggtcttt ttctaactgc ctctaatcct tccaatactt
    11101 ggatggacga gtcattgtat tcgttgttta ctttttttgc caaggcaaac gctccttcaa
    11161 taatactttg cggcaaaacc gcaccacgat tatcatacta gaaaaggtcg tgaaaaaaaa
    11221 gagggtagac gagaaatcaa ttttaattat ctcctaaccg ctttttcgac attcccctct
    11281 atcgatcatc tcttctttgt ggtcattttt atttttggtt ttctttcaac ttaccaagtt
    11341 caatcttaat acaacgatcc attacgatat ctgtacgtcc tgcttttctt agcatctcag
    11401 ctgcttcttt gttttcaatc cctaattgtg cccagaacac tttagcatca gttttcaaaa
    11461 aatcacctgc tatttcaggt aaaaattcac tccgtctaaa tacatctacg atatcgattg
    11521 gatcatcgat ttctgtcaaa ttcgcataga ctttctcgcc tagaatttct tcaccagcca
    11581 ataatggatt tactggtatg attttatatc cattgtcttg caatacctgt gcaaccatgt
    11641 aacttgttcg ttctggttta ttgctcaagc ctacgacggc aatatttttt gcttcttcca
    11701 aatagttgaa aatcgtttct tgtgttggat tttttacaga catttctgcc accccttgct
    11761 tttcttttta ttccatctat tatacttgta tctttcattt tttcaattgt tcctgttacc
    11821 tattcatgct aaaataaagg atagttttca acttgaaaga gggtaaaaaa tgaaattgat
    11881 cttattactt attttagcat atcttttagg ttctattcct tccggtgtct ggattggcaa
    11941 attgtttttc aaaaaagata tccgacaaca cgggagcggt aatactggaa cgaccaatac
    12001 atttcgtgta ttaggcaaaa cagccgggat tgttgtcctg cttatggata ttttaaaagg
    12061 tacattggct acttgtttgc cgatgatctt ccacctttct gggattaacc ctctttggtt
    12121 tggggtatgc gccattcttg gccatacatt tccgattttt gctgggttca aaggaggaaa
    12181 agctgttgcg acaagtgcag gaatgctttt aggatttaat cctgtatttt ttgtttattc
    12241 ttgtatcatc tttatcgtta gtttattttg tacaagtatg gtgagtttga gcagtatgat
    12301 cagtgcagta ttgatcactt tatctacgat cgtccttcct tatgtagctc ctgtgatttt
    12361 ggctaagccc aattggttac tgacgatcat tgccttttta gtttcttcct ttatctttta
    12421 tcggcataaa gacaatatca agcgtatcag aaaccatact gaaagtcgaa tcccgtttgg
    12481 tttgaatagc agcaagaaaa aatcctgata ctttttcatt ccaaagagat aaaacgggag
    12541 tgtgacaaat cgatgtcaca ctcccttatt acttacagga tagctttttt acacaccttg
    12601 gtaaacggtg ttccctgcta acttctcggt cacaacctct tacttcactg tgatggaata
    12661 ttttgtcttg aagatttcat gggcacctag ttgattgatc cctacttttt ccatcaagtt
    12721 tccactactg ttcaccgtgt cagcgattcc gcaccacggt tcgatacaaa cgaaaggcgc
    12781 ttcttttgga taaggagacc agatccctac ataaggcata tctttatagc ttaaagttac
    12841 gctatgggaa gatttttcac ttcgaatggc gtacgcattt agaccccttg tttcaaaaac
    12901 caaggcatct tgctcaaaaa gttcacgatt caaagccaga ttcgtatttg tctgacctaa
    12961 cgtcttttga tccatatcga tgaatgggcc tttcaatggt aggcgtaatc ttgatttcaa
    13021 tggagaaaat gccagataga aatcttcaaa tgacaatcct tcttcaagag gtacattgaa
    13081 agcaggatgt ccaccgatag aaaagaacat ttctttttca ccggtatttg ccacttgata
    13141 atgaaccgtg atgccgtctc ctcctaattc atagctgatc actaattcaa aatcaaatgg
    13201 atacttttct aatgtctccg gcgtactcct taagatgaaa ctagccattt cttgtccatg
    13261 ttcaatgact tcaaaatcca tatcccgagc aaatccgtgt tgccccattt gatatgtttt
    13321 cccttgataa acatactgat catcttttaa acgtccaaca aaagggaata aaaccggtgc
    13381 atgtctgccc caatacgctg gatcaccttg ccagatatac tctaaaccat gttcttttga
    13441 tttcagactg atcaattccg caccgtgttc agcgatttcg gcgatcagct ggtcattttg
    13501 aataataaca gtcattgcca ttcctccaac ttcgacttac tttttaaaat gggaaggctg
    13561 gaacaaacat ttctatctgc cccagtcccc ttacttctaa taactgtgtt ttgctaactc
    13621 gtctttaaaa cgggaattca atacttttaa ataagttccc ttcatgccta acgaacgaga
    13681 ttcaatgatt ccagctgatt ccaattttcg taaagcattg acgatcacag aacgagtgat
    13741 gccgatacta tcggcaatat tagaagcagt cagacgacct tcatctccat ccaatgcttc
    13801 aaagatagct tgtacagcct tcaattcgct ataagataat gtgttgattg ccatttggac
    13861 agccgttgca ctccgtacat ctgcttcgat gcttcgagac ttctgataaa ggatctgcat
    13921 accgacaacc gtagctccat attctgctaa gactagatcg tcatctgcaa acgattgttc
    13981 catacgagat aagataatcg tacccaaacg ttcgccagct ccaaagatcg gaacgacagt
    14041 cgtcagacca aatggatatt tctctcttga ctctaccgga aatgcagtaa tatcactagt
    14101 aattgggata ttagcttcag ttttcatcaa ctgtgccact gtatcagtat aagattcagg
    14161 aaattgtttc tcaacaaaca ttgttttgat tcgttcattg ttcacatcgt gacgttcatt
    14221 ataacctagt aatactccgt cacttcctat gatataggtg ttgctgtcaa gaataccacc
    14281 tagtgtgaca gccattttat cgtaaggcaa atctgcttgc atgtcaaaag tattcttttg
    14341 ctgcaaaagt gtgtttatct tgcgggtttt cgttagcaag tctgtcattg tcgtcctccg
    14401 attttttata aaatatagcg acttaaatct tcatttgccg caattgagcc aagtttgtca
    14461 tccacatatg cttctgtgat cgttatttct cccatttgca tatctggtgc ttcaaataat
    14521 agatcttcta gtagtttttc caaaatggta tgcagtctgc gtgcgccgat attatctgtg
    14581 tcacggttga catcgaaagc gatttgagcg atccgttcaa ttgcttcttt tgtaaacgta
    14641 actgacacat tttctgtacc gatcaacgcg acgtattgtt ttactaaagc attatttggt
    14701 tcggtcagaa tcttcacaaa atcttcagct gtcaaatcat ctaattctac ccgtatcggg
    14761 aagcgtcctt gaagttctgg aattagatca cttggtttag acaaatggaa agctcctgaa
    14821 gcaatgaata ggacgtgatc tgtttggatg atcccgtatt ttgtgtttac ttgtgaacct
    14881 tcgacaattg gcaatatgtc tctttggacg ccttctctgg atacttctcc agattgctgt
    14941 gatttagaag tgatcttatc gatttcatcg ataaaaataa ttccactttc ttccgctaat
    15001 ttgattgcct cactatggat atcagcatcc ttgactaatt ttgctgattc ttccttgatc
    15061 aataactcta aagcttcttt gactgttacc gtccgttcca cttttttctt cggtgtcaaa
    15121 gcacctagag tttcatttag gtcgatcccc atttgctcca atccattgtt catcattggt
    15181 gcaggtttct tttggtcttc aacttcaatg gttacttcac gactatccag taaacccttt
    15241 tgtagttgtt ccaagactgt acggcgattg attcgaatat cttctgtcac ttcttcttgc
    15301 ggttcagcgg tattttgctg actagcatta aaaagattca tcatttgttc aaattgatta
    15361 gtggattgtt tctgttcttt tttgatacca ggtaccaaga tttttaccaa acgtttttct
    15421 gcattttttt tagcacttgt gtatacacgg ctgtactgct ctttttctac gatttggatc
    15481 gcattttcta ctaaatcccg aaccattgat tctacatctc ttccgacata accgacttca
    15541 gtaaattttg ttgcttcgac tttgacaaat ggtgcattga cgatttttgc caaacgtcgt
    15601 gcgatttctg ttttaccgac ccctgtaggt ccgatcatca gcatattttt tggtgtaata
    15661 tcttgttgca ttttttcatc taactgcaaa cggcgatagc ggttacgcaa agcaacggca
    15721 acagattttt ttgctgcgtg ctgtcctacg atatattcat ctaattcact tacgatttgt
    15781 cttggtgttt gttttaattc atacatatct actcacctct tacagttctt ctacgataat
    15841 attatgatta gtaaagatac agatatctgc ggcaatattt aacgcatttt ctgcaatttc
    15901 tttcgcactc atgtcgtttt gagcatagtt tttcattgct cgggctgcag ccaacgcaaa
    15961 attccctcct gatccgatag ctaagatacc atcatctggt gtgataactt ctcctgtacc
    16021 tgaaacaaga agcatttctt tatcattcat tacgatcagc atcgcttcaa gtttttgcat
    16081 agattgctgt gtccgccatt cttgagctaa ttcgacagct gcgcgttgta gattcccgtt
    16141 atattcgttt agtttgcctt caaatttttc ttctaaagta aacgcatctg caacacttcc
    16201 ggcgaaacct acaacaactt ctccattgta gatacgtcgt actttctttg cagtgccttt
    16261 catgacaaca gattcaccca ttgttacttg gccatctcca gccatagcaa atttcccgtc
    16321 tttttcgacg gcacagatag tggttgaatg aaatggtgat tcgaccatgt ttgtttcctc
    16381 ctaaatttgt tcttttcctt atttttacgc tcttggatgg aaagaacgat agtttttttg
    16441 taaactttct tttgttacat gggcatagat ctgggtagtc gataaattgg catgtcctag
    16501 caattcttgg actgtccgca aatctgcccc attatttaat aaatgagtag caaatgtatg
    16561 gcgcagcatg tgaggatgga tatcactatc caaactgctt tttttgatca tttgattcaa
    16621 gacatattca attccagtag gcgtgattgg ttttccatga tgattcacaa aaacgatatc
    16681 atgctcttcg tgatattttg tcatcaatac ctggcggcct tccctgaaat attcttgcag
    16741 agcatcttga gcaaaagaac ctagaggagc atatctctct ttgttccctt taccatggat
    16801 caacatgacc tctgaatcaa aatcaatgtc tgataccgac aaactgctac actcacttaa
    16861 acggatgcct gacccgtaaa ggacttccag taaagcgcgg ttcctcaaat ccaaaggact
    16921 atctcctgct acgctgtcaa aaagtgcttg catttcattt tcataaaaaa aacgtggcag
    16981 tctcaaattt ttctttttga gatgaacgta agaaaatgga ttttctttta tcacttcttc
    17041 tttgagaaga tactgataaa acgaacgtag gctggctatc ttgcgactga tagaatttct
    17101 gctatagcta ggacgatgat cagctaatgc actcaaatag acacgaaccg taaaatattc
    17161 cgccttcagc agatcagcgt ctccagattc ctccaaaaaa tgtttgaaat ggagaatgtc
    17221 ttcttcatat gcttctttcg ttttttctga atatcctctt tcgacaatca aataacgcaa
    17281 aaatttttgt tcccacgtgg cttctgtcat tttctcacct tccttttgtc ccagagaata
    17341 atttagcaca gcgtttataa aaagacaaat aaattgtcag aatttaacca atatttctaa
    17401 gtttgttcac aattggcgca attcctcaca ttttgtttga gaaattctta agaaaaacgc
    17461 aagaaaaaga gcgagactat ccgtctcacc ctcttcatgg agtttcctta ctcatttggg
    17521 attgcagttt tttctaattc tgtttttatt gcctcattag cttttaaagc tcgttgtgca
    17581 attgcttcat atcttgcttt tttgtctcga atgcgctctg gcagttcagg aaacagacca
    17641 tagttggcat tcatcggttg gaaatgtttt ccttccgcat gagtaatgta gtaagccata
    17701 cttccaatag ctgtttcttg tggaaactcg actggttcca tttcttttgc taagcgtgcg
    17761 gcgttgattc cagcaagtag tccgctcgct gcactttcaa catagccttc tacaccagtc
    17821 atctgacctg caaagaacag atcttcgcgt ttcgttgatt ggtaagtcgg tttcagcaat
    17881 tctggcgaat tcataaagct gtttcggtgc atcacaccat agcgaacgaa ttcagcattt
    17941 tctaacccag gaatcatccg gaaaacacgt ttttgctcgc cccatttcaa gtgagtctga
    18001 aagcccacga tgttgtataa agaagctgca gcattgtctt gacgcagctg gataacagca
    18061 taaggacgtt tgccagtttt aggatcttcc aatccaactg gtttcatagg tccgaaaagc
    18121 atcgttttga atcctcgctg tgccattact tcaattggca tacatccttc aaaatatttt
    18181 tctttctcaa aagatttaag ttctgccact tcggcattga ttagtgcctc atggaaagct
    18241 ttgaattcct cttctgtcat cggacagttc aagtaagctg cttctccttt gtcatagcgg
    18301 gacttcaaat atactttatc catatcgatc gtagctttgt ctacgattgg tgcagctgca
    18361 tcatagaaat aaaaaccatc tgaaccattg aatgctttga tgctttctgc taatggatga
    18421 gaagtcaaag gtcctgttgc tactacggtg attccttccg gtatttcagt aatttcttcg
    18481 tttatcactg ttactaatgg atgctctttg acttttcggg taattgtttc agaaaaagaa
    18541 tcgcggtcaa cagctaaggc accaccagct ggcactgctg tttcatctgc cgaagtgatt
    18601 actaccgaat tcaatcggcg catttcttct ttcaataacc ctactgcatt agccaaactg
    18661 tttcctctta atgaattgga acatactagt tcagcaaaat tacttgtgtg atgcgcctca
    18721 gtcgatttga ctggacgcat ttcatataaa cggacaggga ctcctgcttg tgcgatctgc
    18781 caagcagctt cacttccggc aagccctgct ccaacaacat ttaccatttt caatgaaaga
    18841 acctcttttc tattattttt ggatgtcttc ttcgtaatct ccatttacac agactacttg
    18901 tttaccgcct ttgactttct tctcgaccag atatccgtca cattttggac aatttctgcc
    18961 cactggttta tcccaagaag tgaattcaca ttctgggaaa cgggaacaac catagaatag
    19021 tctgtttttc ttagattttc gttcaatgac ttgtccttca tgacatactg ggcaagtcac
    19081 accgatttct tttacgattg gtttcgtatt ccgacaatca ggaaaattac tgcaagcata
    19141 aaacttccca tatttcccta attttatcac catcggatgt ccacagacat cacaatcgaa
    19201 tcctgctggt tcatctttga tttgaatttt ttcgattttt tcttccgcat tcgtcagttc
    19261 tttttcaaac ggtttataga aacgatcaac cactttgacc cacgcttccg tgccttcttc
    19321 aattttatcc aaatctcctt ccatttctgc tgtgaaatgg atatcgacga tttgagggaa
    19381 gaattcacag attaatgaat tgacaatttc tcctagttca gtcggctcaa atcttttttg
    19441 tgtcaatttt acatagtagc gtctttggat cgtatcaagt gttggtgcat aagtggaagg
    19501 tcgccctacc ccgttttctt ctagtgcacg aatcaacgta gcctcactaa aacgtgctgg
    19561 tggttgagta aaatgttgtt ttggttcaat atcaacagat tggactacat cgccttcttc
    19621 aagttctgga agaatatttt ctttctcttc tttgccgtca tctctacctt ccacatacac
    19681 ttgcatgaat cctttgaatt tgatttttga accgttcgca atgaagatga caccattttg
    19741 ttctaatgtc actttcattg tatctaaaat agctggtgtc atctgactag ccacaaaacg
    19801 tgaccagatc aatgtataaa gtttcaattg atctttatct aaatattgtt tgagttcatc
    19861 tggtgtacga agcacactcg ttggacgcac agcttcatgg gcatcttgtg cgccttgagc
    19921 atttttgatc tttcggtgat catgtctaga aaattctttt ccatacgtat cttcaataaa
    19981 tgctgcggcc tctgcttttg ctgtatcggc aatccgggta gaatcagtac gcatgtatgt
    20041 aatcaagccg actgttcctt gtttacccaa agtgattcct tcatataatt gctgtgctac
    20101 catcatggtt tttcttgttc gaaagtttaa cttacgggca gcttcttgtt gcaggctact
    20161 tgtagtgaaa ggattcgcag ggtttctttt gcgctctttc ttttctactt ttttaacttc
    20221 gaagtctgtt ccatcaattc tagcagtgac ttcttttaca ctttgtgcat ctggtaattt
    20281 ctttttctta ccgtcgattc cccagaaatt tgctttgaat ttcttgcgag cttttttaaa
    20341 attaccgtcg atgctccaat attcttctgg aacaaattct cggatctctt tttcacggtc
    20401 aataataatt tttaaagcga cagattggac acgtcctgca cttaaccctt tcttgacctt
    20461 acgccagaga ataggactga tcgaataacc aacgattctg tctaaggtac gacgagcttg
    20521 ctgtgcatct actaaatcta catcgatcgt tcttggttcc ttaaaagctg ctttgactgc
    20581 gtcttttgtg atttcattga agaccacccg atttttttct ttcagatcca acccaagtag
    20641 gtacgctaaa tgccaagcaa tcgcttctcc ttctctatcc gggtcacttg ccaagtaaac
    20701 tttttgagct ttttttgctg cggctttcag gcttttgatg acatcgcctt ttccgcgtat
    20761 agaaatataa tggggttcat aattgttttc aaaatcgata cccattttac ttttcggtaa
    20821 atctcttata tggccaacac tggccattac tttatagttt cgtccaagat atttctcaat
    20881 tgtcttggct tttgcaggtg attctacaat cactaaatat ttatacgcca ttactgaatg
    20941 gactcctttc cattgtcgtg ttcctactat taaaagagga ttcatacaaa tcgtagctaa
    21001 tcatatccat tcagaaaacg tttgtcaaga aaagcaaatg aaaagtatgg tgttattctt
    21061 taaattctgc caagatatct tcacccgaaa ccgtgcaaac tgcaccgtct tggatcaaat
    21121 gatgacatcc ggtagaatgt tctgttaaaa tatttcctgg aacagaaaaa actgttcttc
    21181 caagatccaa tgcttgctgt gctgttatca gagagccgct ttttttcttg gcttcaaaga
    21241 cacatgttcc tttagacaaa gctgcgataa tccgatttct cattggaaaa caatgctttt
    21301 tcgggccgga ttgaggagga tattcactca aaagaagatg atcatttgca atcacctctt
    21361 gcaagtcacg attttcagca ggatagactt gctctatccc tgttccaatg actgcgattg
    21421 tccgtccacc atgagagatt gctgcttggt gaccgtatgt atctatccct ttcgcgcagc
    21481 cactgacgat cacatatcca gcttctatca ctggttttag taaataataa actgtttttt
    21541 tggctaaagc agaagtgtgt cttccaccaa caacgcttaa catgttacaa gaaagtaaag
    21601 atagatcacc attataaaac agtacaattg gcggataggt caagtgaagc agctgagggg
    21661 gatatttggg atcaaagcaa gtgataaacg actgtttcga ttggattttg ccccagttag
    21721 cgtgaatttc ctgccaactt ttgcgaaaca gctcccggtg tttggttatt ttacctatct
    21781 taatgatgtc ttcaaatccg atttctttat aaggatactg catcatccag ttagcaagtg
    21841 cccattttcc ttgactactg attccttttg tataagcaag tttcaataaa ttttcttcta
    21901 tttgatacat cataaaaccg cctttcgtct ctctttctaa taagatacgt aaaagcggct
    21961 gttttttatt gtaaatattt ttttacaggt gcaaaacttt tacgatggat tggcgtaatt
    22021 ccttgtcgtt caagtccttc taagtgttcc tttgttccat aacctgcatt atgcgagaaa
    22081 ccatatccag gataaagcac gtcatattct ttcatcaatt ggtctcgata tactttagca
    22141 atgatacttg cagcagcaat cgacacagat cgtgcatccc ctttgattat tttttcctgc
    22201 tgaataggca aatccaaggt catcgcatcg atcaacaaat agcttggtac aatggacaat
    22261 ttttctactg cttggatcat cgctaattta cttgcttcat aaatattcac atcatcgatt
    22321 gtctgctgat caacgatccc gatcccgatt gccagggctt tttcttgaac ttgttgataa
    22381 agaagttctc gctttttctc tgataattgt ttggagtcat tcagccctaa tatgatctca
    22441 tttttcggca agataactgc agctgccacg acaggtccag ctaatggtcc tcttcctact
    22501 tcatcaatgc cggcaatcca ttggtgacct tgtgcgtacc cttgcttttc aaagcaaaac
    22561 atttcttcat atttttcctt aattaatgct tcctttttca atcgtcgttc aaaagaagcc
    22621 agtgcttgct gcacgccttt gcgtggatca tttcttaatg attctagtgt aggatcatct
    22681 attctctgta tgcttttcaa tcgttctttg atactttgga tggattcagt catcactttt
    22741 ttgctccatt tcttcgcaac gatccagtgt gtatcgtcca agttttcctt gacgaatatc
    22801 atgcacgact aattcgctgc cacgatcata gtcttcttta tacccacgtt ttgctgtaat
    22861 atccatcagt aattccggtg ccaatttttc ttcttgctcg attgataaac cataacgatc
    22921 gctgattctt cctgaataat atcgggcaaa aaagtctaat ccgtaaatcg ctaaatcatc
    22981 taaatggaca agctggtctt tgatcgcgcc tgtcagcgct agttttttgc cgatttcttc
    23041 atcttcaaat ttgggccata aaatccctgg tgtgtctaac aattccaact cattacctaa
    23101 acgaagccac tgctgacctt ttgttacgcc aggcttattt cctgtctgtg caattttctt
    23161 gcctactagt cgattcatca acgtagattt cccaacattt ggaataccta agaccattgc
    23221 ccgaatagcg cgaggcttga tcccccgagc agcatctctt gcccgttttt ctttcaatgc
    23281 ttctttagcc gcaggtacta gacctttaat tcctttactt tcttgtgccg tgatagctaa
    23341 tgcaagatgg ccttgtttac gaaaatattc ttgccacagc tttgtctgtt gtggatctgc
    23401 cagatccgct ttattcaata aaacaaggcg aggtttttgc tgaatgatct gatctagcat
    23461 tggattacga gaagataaag gtaatcgtgc gtctaccaat tcaaatacga tgtccacata
    23521 tttcattttt tcagaaactt ctcgtcgggc ttttgccata tgtcccggaa accattggat
    23581 ggtcattgtt ctctccattc ctttcatacc ggaatattcc ccggctttac ttgttttttt
    23641 ctttttcaat ataaattcac ttttttatta taaagaaatt ccatctatta gttaagtgaa
    23701 agtgaaccag tcctcattct atcacatctt cacttttttg atg
//
LOCUS       DANKKG010000032        23600 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000032 DANKKG010000000
VERSION     DANKKG010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.032"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            334..1317
                     /locus_tag="PW004_001064"
     CDS             334..1317
                     /locus_tag="PW004_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HDL2726052.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            complement(1685..3823)
                     /locus_tag="PW004_001065"
     CDS             complement(1685..3823)
                     /locus_tag="PW004_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL2726053.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(3862..5448)
                     /locus_tag="PW004_001066"
     CDS             complement(3862..5448)
                     /locus_tag="PW004_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDL2726054.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            complement(5438..6658)
                     /locus_tag="PW004_001067"
     CDS             complement(5438..6658)
                     /locus_tag="PW004_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2726055.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            complement(6670..7476)
                     /locus_tag="PW004_001068"
     CDS             complement(6670..7476)
                     /locus_tag="PW004_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2726056.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            complement(7686..8966)
                     /locus_tag="PW004_001069"
     CDS             complement(7686..8966)
                     /locus_tag="PW004_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HDL2726057.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            complement(9010..11043)
                     /locus_tag="PW004_001070"
     CDS             complement(9010..11043)
                     /locus_tag="PW004_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726058.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            complement(11077..11928)
                     /gene="rfbD"
                     /locus_tag="PW004_001071"
     CDS             complement(11077..11928)
                     /gene="rfbD"
                     /locus_tag="PW004_001071"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HDL2726059.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            complement(12026..13054)
                     /gene="rfbB"
                     /locus_tag="PW004_001072"
     CDS             complement(12026..13054)
                     /gene="rfbB"
                     /locus_tag="PW004_001072"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HDL2726060.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            complement(13076..13648)
                     /gene="rfbC"
                     /locus_tag="PW004_001073"
     CDS             complement(13076..13648)
                     /gene="rfbC"
                     /locus_tag="PW004_001073"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HDL2726061.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            complement(13662..14528)
                     /gene="rfbA"
                     /locus_tag="PW004_001074"
     CDS             complement(13662..14528)
                     /gene="rfbA"
                     /locus_tag="PW004_001074"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HDL2726062.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            complement(14628..15362)
                     /locus_tag="PW004_001075"
     CDS             complement(14628..15362)
                     /locus_tag="PW004_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL2726063.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            complement(15340..16167)
                     /locus_tag="PW004_001076"
     CDS             complement(15340..16167)
                     /locus_tag="PW004_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL2726064.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            complement(16167..16967)
                     /locus_tag="PW004_001077"
     CDS             complement(16167..16967)
                     /locus_tag="PW004_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL2726065.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            complement(16960..18099)
                     /locus_tag="PW004_001078"
     CDS             complement(16960..18099)
                     /locus_tag="PW004_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HDL2726066.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            complement(18304..19497)
                     /locus_tag="PW004_001079"
     CDS             complement(18304..19497)
                     /locus_tag="PW004_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2726067.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDALGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRTNVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVIENDTKEKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQRVINSRPKIKSIEVDGHSGFVFINPKGNPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(19576..19782)
                     /locus_tag="PW004_001080"
     CDS             complement(19576..19782)
                     /locus_tag="PW004_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HDL2726068.1"
                     /translation="MSNTQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEKVIDKLDAI"
     gene            complement(19775..20917)
                     /locus_tag="PW004_001081"
     CDS             complement(19775..20917)
                     /locus_tag="PW004_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDL2726069.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYRNGGAECRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVEFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDISDVIESEGVFYE"
     gene            21429..22064
                     /locus_tag="PW004_001082"
     CDS             21429..22064
                     /locus_tag="PW004_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2726070.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            22406..23041
                     /locus_tag="PW004_001083"
     CDS             22406..23041
                     /locus_tag="PW004_001083"
                     /inference="COORDINATES: protein motif:HMM:NF012655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2726071.1"
                     /translation="MARNKYPEETVKLILDVSTKMFCEKGYDNTSLQDIINETKLSKG
                     AIYHHFDSKEDILKAIFQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTAVFNSNQSV
                     LLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMV
                     LSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            23148..>23600
                     /locus_tag="PW004_001084"
     CDS             23148..>23600
                     /locus_tag="PW004_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014073946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2726072.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLAN"
BASE COUNT         7171 a         4810 c         4066 g         7553 t
ORIGIN      
        1 tagtcacctt taaaaaccta gtttataagc aaattgtaat ggaatttaaa aatgtctaat
       61 aaaaagcttt atacacctct aatcaagaca aggactctaa attacaaatc tccagatgaa
      121 tcgattttca ctaatgttgg taaattaaag aagtcgaagt atttgctgct ttttttatcc
      181 actcataaag tgaaccttta tatttttcag agaatccatc ttcccaaagc gctgtctagt
      241 ccataagaat acctgtatta cgtgaagaat cgaaaaaatt gaaatcgatt tttaatgaca
      301 ttatgcttat agaagaaagt gaggtaatat cttatgaata aacaagagtt gcatgatttt
      361 attggttttc gacaaccaaa tatatgtcaa attgtcgcat ataaggatgg ggtagaagtt
      421 tatacagatg aatggaatgg ttataaaaaa gacgatactt gtcatatcat gtcagcaacg
      481 aaaagtattg tatctttgct gattggcatt gctttagagc aaggattaat caaaagtatc
      541 gatgataaag tattagatta ttttccagag tataaagtga aacgcgggga gaaaactatt
      601 tacgctatta caatcaagca cttgctaaca atgaaagcac cctacaaatg taaaggcgat
      661 ccatggacaa aagtctgttc aagtgaagat tggacaaaaa catcactaga tttattggga
      721 ggaagaaaag gtataaccgg cgaattcaac tatcaaactg tctgtttaca catattaact
      781 ggcttgttgt atcagacaag taagctaaca actgttgatt ttgccaataa gtatctattt
      841 gatcctatag ggataccaaa ccatgtcaat tattatgctg aaacagctga agaacataag
      901 cagtttacaa tggataagtc tcctaaaacc aatgtctggt tttgtgatcc actaaatatc
      961 gggacggcag gatatggttt gtgtttatcg gcaagtgatc tggcaaaaat tggtgtatta
     1021 tgcctaaata aagggatgta taaaggaaaa caaatcgtac cacccatgtg gataaacgag
     1081 atgttcaaac ctagaaccag tgctttagaa gacttacaaa atatgagata tggctattta
     1141 tggtgggtat tagattctga gaacgaaata tattcagcaa ttggtaatag tggaaatgtt
     1201 ctttatattg acgctaagaa caatctggtt atctccatca cttcttattt taaacccatg
     1261 ataattgata gagtggcttt tgtagaggaa taccttaaac catttatttt aaaatagcct
     1321 atattaaaat aaaatatcga ttctttgaca gctagggttc gtgaaacttt ggctatgatt
     1381 ttgtatctat tggcgctctg agcatgaata agagcgtggg acaaaagtaa gaaacacttt
     1441 tgtctcattc tttaaaaacg aataaacggt gggagcagaa gcaacttctt cttaaataag
     1501 ccgaaattca caaaaatttg aaaagcaatt ttcgtgaatt tcttcttatt tctcggagtt
     1561 aaacacttct gtcccaacct cgacttattt ttttgagtcg gcaccttctg cctttttttg
     1621 acaaaaaact ataaaaagcc gttatgtgcg agcgaatcac aacataacag ctttttatta
     1681 tagtttaatc tggtctcaac gaaaagtcgc cgattccttc ttttgttaaa ttatcattgt
     1741 aaaatggatc atgcgcaata tatgctggcc atttatcttg catttttttg atttcaccag
     1801 caaaacgtct ttgtttttct ggagtatctt cataacctct tgatttagat tcgaaatggt
     1861 acaattctgc ttgatgtgca tagacattgt aacgacccag ttcataaact ttcaagcaca
     1921 agtcaacatc gttgaatgct acttctaatg tttcatcaaa cccgttaaca gcattgaagt
     1981 cagctgcctt gaccatcata caagctgcag tgactgctaa gtaattgacg tcaatgacca
     2041 atcggccgaa ataaccacaa tgtgttcgat catagttatt caatgcatgc cctgcaacac
     2101 caccgattcc taataatact cctgcatgct gcgttgtatc atctggataa aacagttttg
     2161 ctcctacaca gccgattcga tcaaactgtg cataagaaac catcaccgtc atccaatctg
     2221 gctcaatcac ttctgtatcg ttattcaaga ataaaaagta tttgccgttt gctttttctg
     2281 ctgctaaatt gttaattcga gaataattga atggaatgtc aatcaattct acgataaagc
     2341 ggtctttcag ctgatgcttg tattctgcaa ataattcttg catttttgga tctgtacttc
     2401 cgttatccgc aataatgatt tcataatttg aataagacgt tttttcgatg atcgaatcca
     2461 cacaagtttt tagatcttca tagccattct tcgttggaat gatcacactg accaaatcct
     2521 cttgcaagac gtcataagca atctcataaa atccagaaat tctgccagga cgtacgcttc
     2581 ctttgatgtt tcttctagac agggcatctg tcaatgcttt cactccggaa tcatagatat
     2641 aacttttggc ttcgccatta cttgcagttg atcctgggat cgtccgccaa tgatacaaga
     2701 ctcggtcaat gtggtaaata tgatctgcag ggatttgttc tgtcacacga aggaccaaat
     2761 cataatcttg agaaccttca tagcctttac ggaaaccgcc aagttcttta acaatgcttg
     2821 ttcgatatac gcctaagtga gagatataat tatttcccat caatgtatca ggcgaccagt
     2881 ctgctttgaa atgcggatca aagcgattgc catctgcatc gattttatct tcgtcactgt
     2941 agatcacatc cagttctgga tgtacgttta gcacttttgc cacttcatat aatgcaaatg
     3001 gcggtaattc atcatcatta tctaagagtg ctataaattc tccttctgct atttccaatg
     3061 cagaattagt tgccagactg atgtgtccat tttcttttcg gaaaacgact ttgattctat
     3121 catcttttgc ttgataggat tccagacact ttcggatagc tggatcagta gaagcatcat
     3181 cactgataca tagttcccaa tgatcatacg tttgatccag taccgaatca atgcattttt
     3241 cgagccattt gatttcaacg ttatagacag gcataagtat ggaaatcaat ggacggtacg
     3301 caaatccttg tgcttctttc ctttgctctt tcaaatctgg tttctcatga cgactgatcc
     3361 actctgcata gtcgatcgat cctggcttca attccaattt gacccgacgg atcgcttttt
     3421 ttacgccatg agtccttgca taatttatcc cttttttcag taatcttttt tttctttccc
     3481 atgaagattc aatgccgtca tcataagggt atttaccgtt taatttcaca gctatgccat
     3541 tttgatgtct cgctgtttgg aagtctaaga tggcttttcc tctcattttc cctgataaac
     3601 gtaccttaaa accgctttgg gtcttaacat caagattata taaatgattg atatccaatc
     3661 ttacgacatg tgttgcttca gcaatcacat tttctttttc tacctttatc gtagggattt
     3721 ctttggtcac ttcatcaatt gcccagccca caataagcaa atcgccagtg gcccgttcac
     3781 gagtgatttg atcgataaat acggcaattt cttttttttg cacgattcgt ttactcctta
     3841 tctttataag ttaatcatta tttacatttc aatgatccat gattggccat tttgagctac
     3901 tgcttccact aagaatgaac gttcttgctc gcaaaggatt cgtaactcaa atcccgcttc
     3961 tgttcctttt gcgagctgtt ctgcttgatt gacttcttct cgtgaaacag gaattgcttc
     4021 gaaaaatggc gattggtcag ctgataactt ataggacaag ggctggcgat ccgaattagc
     4081 gattccccat ccgatgattt ttaatatttt tgtttctgca tcccagattt tctcgtcaat
     4141 acatatagaa agttcttttt cttggatttt tttcgaagtt tctttcttac gtcgtcctag
     4201 tctatgtttt attgtccatt catcactttc caacaatgtg caatagcgat ttgtggttgt
     4261 ttttaacgta tggtctagtc ttgtattttc ctcaagaaga tgatgatttt ctctttctaa
     4321 ttcgtcattt ttctcagaaa gctgctttgt gacttcagcc atgggacgaa tcgtttccaa
     4381 caattctttc attgttggtg tcaattcccc gataaagcga tagtgacaat ggatagtcac
     4441 ttctttccca gaaatatccg ttagaacgaa ctctggtaat tctttcccat caaacaaata
     4501 acaatcattt actgttttga ctacagcatt cgaatcgata aatgttagtt tttctccggc
     4561 agcttctgcg ctgaattcga taaagctagg ttgttgcgcg aagcgaaaca ccattctttc
     4621 tgttgtttct gatacgggga aacttagtgt ctcattttcc cctgtgaaat tattgaatgg
     4681 tatttgttgg attgtttcat cttgtttcgt ttgctgggtt acttctaaca cttttacata
     4741 attagaattt tgtggctctg caatggaact ttgtgctact ggatgtttca tcaatgagaa
     4801 aaagtattgg tagacttcac caaatggtcg cattttgaaa tcatagcgta cagcttccgg
     4861 caattcttca taagttgatt ttaattctgt atcacctaca gctaagtaca aataatcttc
     4921 aatattaatg aataatcccg ctttttcaaa aactttttgg aagccatcat gagtataaaa
     4981 agaattatgg gtttcatcta ggagaccata agaagcccac ggcaattggt tattgaacaa
     5041 atcgatcatg acagaattat gcgccaagtt agggaatgta atcaggatct ctccttcttc
     5101 attcaaaaat tctctgactt tcttcaatgt ttttccagga tctaccaaat gttctaaaac
     5161 atccgcaaac aagacgtaat caaatgtctg tccagcataa tagtttgccc actcaaaact
     5221 ttcgatatca ccataaaatc catcttgagc aaattcgctg acaaaatcga acaattcctt
     5281 atctaactca acaatgctca cttcacattg ttttgctccg attaaatgtt tcgttaatcg
     5341 gccgtttccg ggaccaaact ctaaaacttt acttcctggt ttgatctgtg cggcaatttt
     5401 tcccacactg ctttgttcat ctaggtccat ttcaaaatca tatttcattg ctatgagtcc
     5461 cttcttccca tttatggtct aagaccacga tgccttctcc gatataagaa ccgctgacaa
     5521 acaagttcat gtactttgtc ttatatacca aaggaaccgt tgcattttct tcaaagaccg
     5581 caacgtcaaa ataatattca ccagctaaca aacctaactt ttcatactcc agataaaaga
     5641 tattctctcc ttttttccaa ggaatcttga taccatctag tagagtatta agtccgcata
     5701 cataaaaatt gtcgactgtt cgtagcgcaa ttcctaaaac cggatttttg attgtatcat
     5761 cttttacttc ataagttact ttaacgtata gtttttctcc ttgttccagc atatccaccg
     5821 gatttttgtc tttatccaac aattccgctt tcgtcacgtc gacgatatct ggagcggcga
     5881 accgttctac ttggttttta ttccgatcca ctgttttaac tgattttttc tttaaaaagt
     5941 tttcataatt cgtcgttact tccatcgtat cgccatattc gacgacttct ccgtttttca
     6001 accaatagga acgatcacaa aaacgacgaa tcgaactgat atcatgagaa acgaataaaa
     6061 tcgtctttcc cgagttcttg atttctgtaa atttttccat acattttagt tgaaattcaa
     6121 gatcgcctac tgccaatgct tcatccacaa tcaaaatatc gggattcaca ttgattgcta
     6181 cagcaaaagc tagacgaacg aacatcccgc ttgaataagt ttttacaggt tgataaatat
     6241 ggtctccgat gtcagcaaat tcaatgatat catcgaccat ttcatcgact tcttctcttg
     6301 aaaatcctag aaccattcca tttaaataga tattttcata gccagaatac tcaggattga
     6361 atcctgatcc tagttctagc aaagctgaga tttttccttc gatttccatc gtgccggatg
     6421 ttggtgtcag tactcccgta atgattttca acattgtaga ttttcccgat ccgttttctc
     6481 ccacgaaacc gatcatctca ccttttttta tttctacatt cacgtttttc aacgcataaa
     6541 atacatcatg ataggatttc ttaaaaggat tgagggcttc acgaaatcga tccgaaggtt
     6601 ttgcgtacat attataagat tttgtgatat cttttagtct aattgcatat tctgtcatag
     6661 ctattctcct tacagcacat ctgaaaaatg tggtttcaat ctacggaaca ctgcagagcc
     6721 gattacgaac attactaacg tgaaaaccca gaaatacaaa gtatatgttc catgctccca
     6781 gaaccaagct tcacttaaaa atgactcacg ataaccttga acaacatagt acagcggatt
     6841 taatttcaat atgcttacaa gacctgaatc aaactgatta gggctccata atatcggtaa
     6901 tgtccacatg atcaactgca gcacgatcgt aattaattgc atgatatcag gtaaaaatgg
     6961 ttggatcgat gccgtcagcc aagtcaatcc tgtcaagaaa gcacacatgc aaaataaata
     7021 ataaatcagc tgtaaagaca ttgctgaagg atatatacca tttataagcg cgacagctat
     7081 gccaatcaga atgaagaata agtgggtgta cagattggat aggattttcg tcgtgggcaa
     7141 gatacgaata ttgaatacga ctttctttac taaataagta tattcccgaa aaacaaccgt
     7201 agttgatgca aatgcatctg agaagaagaa ccagggaatc atcccggtaa ctaaatatac
     7261 gataaatggg tattttgcat cgatcatttg tgctttcagt ccaacaccaa ataccagcca
     7321 ataaatcaat acagtaaata gcggattgat aaacgcccat aagattccta aaaacgatcc
     7381 tgcatatctt gatttgaagt cattgatcga aaactgtagc aataattttc tgttctgata
     7441 aatgtccttc ataaataaat acaaatcttt aaacatttgt gttcctccaa gcgattttca
     7501 gtgaattaca gtcaagtgcc attttaccat atttttggta ccgagctgcg ttcttatttc
     7561 tttcttaatt ttctatgata caagattttc tctaacgttt ctattttaca gaagatgcta
     7621 gacaattgca ataaaccaaa aaaggctgcg acggttgtca cagcctctct cgtattttgt
     7681 gcctatcaat gatagcgttg gatcctggaa gaatgagcgc tatctggttg ttcctcataa
     7741 gatgtatcat cataagtcat atcgtcatat gcctcttcca taacatagaa ttcctccgca
     7801 acttctggtt catcatatcg atgaacggcc atagccaaca ggatataaat aatcggaacc
     7861 catgggtatg aagtcaatgt atcctccata aatgactgca tcatcaccgc aaaaaaggcc
     7921 cagataaaag tcatctgccc gttggataaa tctgctttca acgttaagcg accaataaaa
     7981 aggataagca gcaaagcagc aatcccaaat cctatcaatc catagatatt caaaatagaa
     8041 ttataactat tgtggtttcc taaaaattgg tttcctcgtt ggaagataaa tgttttcatt
     8101 cctagtaaaa tttgttcaga tttttctcct agcgtctgat agaatttatg ccaaatatct
     8161 tctcgaccgg taaacaaatt gactttttca gagacagccg ccagataaga aatcggcaaa
     8221 gctaaaagaa tcgtaaccgt gtaagcaata aatggcgcac gaatgacacg catcaacgat
     8281 ttaggtaaaa gatccaatat cgcaaaaaca atcaacgcga ctaatgcacc tctagattgg
     8341 cagacccaaa tcccgagaaa agctgcaaca actgctactg caccgatgat tcgtacatac
     8401 catcgttcaa aagaactggc aaatccactg atcaataaag caagagtcat caaagaactt
     8461 ccgattatat tcgtattgat ccaaatatca gaaagcgaat tgcctggagc ccagatgctt
     8521 tgtccttctg gtacttggat ctctgtaaat acacgatata acgtgattcc gaggaagacc
     8581 gaaaagacaa tcagataaat accgcgatcc cattttgtcc actcgatcca tttaattact
     8641 aaacaaaata aaataatcaa aggaaacagt aagtctccaa ataaagactc atttcttaat
     8701 tcccgaaagt aagaaaagaa aatgaaaagg gcaagcgcaa cacctagata aagaaaatcg
     8761 acgccttttg ctttataaac atttaaaatg agcaaaatag ccacactaaa agcgataagt
     8821 aatgaactat tgttatatat aaacaaagtt gctggaaaac ctaaccctgc tgtccaaaca
     8881 aaaaagacta gcgtcagtaa caaaagcaag aaaccgacac tcattttgtt gacaaatttt
     8941 tgtacgattg cttgaattat ctccactctc ctttctacta acatttcagg attattctca
     9001 gctagcctat tactgtacga ctagctgatt ttcctgagtt tgtgtttgtg gtacatctgg
     9061 ataatccgga tattctactt taaaaatcat ataaccgtct ttatccggtc catatagctg
     9121 cgtgaactgc gcttcatata attggttgta atcagttaat ggtcgtttac tgatcacata
     9181 gtcgatccct aatttttctg cgtcttcaaa attcaacgtg accgaaaaca tatctggacg
     9241 atctaaaatg aactttgtat cttccttagc gataaaagct cttgtatgag cataccgatt
     9301 ataatacttc tcgtattctt ctttcggatc gattttcttc catgcagcta tatctggata
     9361 aaagcgtact gtattgaagg atttcactcc caatgttggg gtgaagttat ataattcatc
     9421 ttcagacaac caaactttac caggatcttg ttgatcgatt ttttggattt cttttgccag
     9481 tgttttttca aaaatcgctc ctgtaccttc tacgactgga ttgacgaaaa agccagatac
     9541 gataactaag gcggcaaaag cgaggtaaaa caagcgtctc cagccaaata atgcaaaagc
     9601 taaaatcact gttcccaaca cagcaactgc gatggtttca aaatccgaaa aataattatc
     9661 cattttcgaa tgcgtaatcg catagaaata agaccatgca tttactgcca acaaaatgaa
     9721 tttataccaa ataggaataa catcttctct ccaaatataa gcgataaacc agatcgataa
     9781 taaacaagcc gcaaaactaa aagtaaggat ggctcttaat ggaggaacat aagacagcag
     9841 agtgatttct gctagccatt ttggcaaccc aacggtaatc caaaagatag aaaacagaca
     9901 aaaaatcatc aaaatacgtc ccagtaactt ttgttcgcta tcttttttgc caaataacac
     9961 aaatggactt gctaggaata cagttggaaa aaagtgatag aacagggcca cttcagagtt
    10021 attcgaatag ttgatatctt caaaaggaat tttccaattt gtaaggaaag aaaaaaattc
    10081 gccaatcgtc cactttcctc cagtacttac ccgttttcct ggatataagg tattcaaaga
    10141 agctaacaat gcgtcttttg acgttagcca gaaatgaacc atgactccgc caataaataa
    10201 aactgcccct ccaatcaaca ctacatccaa cagatcccat gaaactttct tgctatagta
    10261 aataagcagg ccaacaaaat aaaaaatcag caagtacgcc agcatcactt gatgggctgg
    10321 gtaaatcact aaaataaacc caataccgaa aataaccacc aaaagcatca tcaacgttcg
    10381 tagccatttt ttatcatgat tgtagaaata atgataaaaa gcagtcatca gtcccaaagt
    10441 aaagaaaatc aaatcgccca catgttgcat gaaccaccac tggactgctg gagaaaatgt
    10501 cagcgcaaat gcaccgatta gcgatagagc tttttttctc ttcgttaata tcatcagtac
    10561 ttcgaatcca agcaagacca tgacgactaa tttcatcgcc caataaaagg aaagtcctcg
    10621 atctcttccg aggaaaaaga atccccagtt gaatggtttt ccgataaccg taatatcctt
    10681 tacaggagaa ttatacgcaa tgatcatgtt ttgtccgttt aatgaataat cctcgttcac
    10741 caacggaaag tcctcttcag cttgtgagag gtaaaaaggt gtctgcacca gccattcgtc
    10801 tgaccgcact tcccgattct gtccaaaaat cacatcagtt tttttcccat cgtctctttg
    10861 cgacacatat ttatcccaaa caccaagtga acttccgttt aaattaaaag taacgatgac
    10921 cacgatgact aaaacgccaa taagatagcg gagttttaga aacaggtcaa gcaccaaatt
    10981 cgaatctgcc tggttcgctt ttggcttttc ttttcgtcgt gcacttcttg tttgtttttc
    11041 catatcatgc tccaaattct atttgataaa aggaaattac ttattcattt ttttggcagc
    11101 ttccagcatt tgcgcaagtg cttcttgcca agttggtatg acaaatccta actcttctgt
    11161 ctttttcagg ctcatgactg aatactgcgg acgtgttgct ttttgcggaa attcttctga
    11221 cgtaacaggt gcaacttcta catctgtatc tttcaatatt tcttttgcga attcatacca
    11281 agaacagcta ttttcgttcg acaaatgata aataccaaat ggtgcttttt cttcgatgac
    11341 atataccata aattctgcca aagtacgtgt ccaagttggt cgcccaaact ggtcatttac
    11401 tacagtcagt ttgtcgcggg tttccgctag gcgttgcatc gtgaagacga aattgtgtcc
    11461 atagatcccg aaaacccaag aagttcgtat gatataatag tcttctaaaa tctcttctac
    11521 tgctttttcg cctaataatt tcgtacgacc atattcattt agtggattcg tttggtcgtc
    11581 tacttcgtat tctacatttt tgttttttcc atcgaaaaca tagtctgtac tgatatagac
    11641 gaattgagca ttcacagctt ttgctgcaag tgctacattt cttgtaccat caacattgat
    11701 cttctcatct aattcttttg cttcttcttc tgctttatct actgctgtgt aagctgcaca
    11761 atggaaaata acatcaggtt ttaattctgt gatgtaagcc attgttttct cggcatctgt
    11821 gatatccaag tcttttgcat ctgttgaaac atattgcaga tttttttcat ctaataaatg
    11881 gcgcaattct gtacctagtt gcccatttcc tccagttaat aaaaacatat gattatccgc
    11941 tccttgattt ttattttaaa aaagagacag gcgagcgcct gcctcacatg tgtttttcca
    12001 ttaatacagt gacattttct ctgtattatt gtccattttt ggcatatgct gcttcaacgg
    12061 cctctttttc ttttttccac cagtcttcat gttcttcgta ccatttgatc gtgtcagcca
    12121 acccatcacg gaagttcgta aactcaggct tccatcctaa ctcttcccgt aattttgtcg
    12181 aatcgattgc ataacgcaag tcgtgtcctg cacggtcgtt tacatggtcg tacgcatctt
    12241 ttggttgacc catcatttct aagatcaatt ccatcacggt cttattatct tcttcaccgt
    12301 ctgcaccgat cagataggtt tcgccaattc tgcctttcgt caaaatcagc cacactgctg
    12361 aagaatgatc attcgtatgg atccagtcac gaacattctt tccttcgcca tataattttg
    12421 gacgaatacc gcttaagata ttcgttactt ggcgaggaat aaatttttca atatgctgat
    12481 aaggaccata gttgtttgaa cagtttgaaa tggttgcacg taagccaaaa gaacgcaccc
    12541 atgcttttac taacagatct gaaccagctt ttgtagaaga atatggactt gaaggattat
    12601 atggtgtttc cgcagtgaat ttttctcctt cgccttcccc atgtcctggc aggtcttcac
    12661 gtaatggaag atcgccgtaa acctcgtcag tagatacgtg atgatagcgt acatcatact
    12721 tgcggcaagc ttctaaaagt gtatacgtac caatgatatt cgtttgtaca aatgggaaag
    12781 gatcttttaa tgaattatcg ttatgggatt ctgccgcgta atgaacaact gcgtccgctt
    12841 cttttactag acggtccact aattctgcat ctgcaacatc tcctacgacc aattctacgc
    12901 gatcgctagg aagtccagcc aagttctctt tgtttcctgc ataagtcagt ttatccaaaa
    12961 cagtgacatg cacttctgga tgattgttta ccacgtaatg aacgaaatta gagccgatga
    13021 atccggcacc tccggtaaca atgatgtttt tcataagaaa atttctcctt aattcttaga
    13081 tttctccgta aacaaatgga ttttctgctt caaattcttt taatgtcgga tgacggtgat
    13141 ctttttccga cataatcgct cttgatggat caattggcca atcaatcgct aaatctggat
    13201 catcaaatgc gatgccccca tctgcttctg cattatagta attatcacac ttataaagga
    13261 agttcacatt tggtgtcaaa gtaacgaatc catgggcata tcctttaggt acaagcaact
    13321 gtcgatgatt atgttcagat aagataaatc cgtcccattg accataagtc gggctacctt
    13381 tgcggatatc cactactaca tctaaaactg ctccagtgac tacccgaatc aattttgtct
    13441 gtgcagcttc tcctttttgg aaatgaagcc cccgaaggac accagcttca gctgatagtg
    13501 aatggttgtc ttgaacaaag tcatatttca atccatgttc tgcaaatttt gcttttgaat
    13561 aactttctgt gaagaaaccg cgatgatcgc caaagacgtc catttctacg attttgacat
    13621 ctgttaattt tgtatctgtt actttcatct gtaaggactc cttattcagc ggccaaattc
    13681 aataaatatt gaccgtattg attttttttc aatggctgtg caagggccag caattgttcc
    13741 ttatcgatgt agcccatacg gtaagcaatt tcttccaaac aagccacttt taagttctga
    13801 cgtttttcaa ttgtttcgat aaaggtagat gcttcaagca atgattcatg tgttcctgta
    13861 tcaagccaag caaatccgcg tcccataagt tctacggata gttttccttt atccaaatat
    13921 gctttattga catccgtaat ctctagttct ccacgttctg aaggtttgat acctttagcg
    13981 atagaaatta cctcattgtc ataaaaataa aggccagtta cagcgtaatt tgattttggc
    14041 tgagcaggct tttcttcaat cgacaaagct tgcatattct catcaaattc tactacacca
    14101 aagcgttctg gatcattgac atgatagcca aatactgtcg cgcctgtatc ttttgaagct
    14161 gctcgttgca acattttaga cagaccgcca ccatagtaaa tattgtcccc taataccaag
    14221 cagacactat catctccaat aaattcttct ccaatgataa acgcttgtgc caatccatct
    14281 ggactttctt gtactgcata ttcgatctgc agtcctaaat catttccgtt tccaaataat
    14341 tccttgaaac gtggtgtatc ttgtggcgtt gaaataatca gaatttcttt gattcccgcc
    14401 aacatcagag tagacattgg ataataaatc attggtttgt cataaatcgg catcaattgt
    14461 ttcgatgttg cctttgttaa agggtacaga cgtgttccgc tgcccccagc aagaatgatt
    14521 cccttcatgg atatctgctc tcctttttga tttcaaattt cactttgtta ttatctcatc
    14581 aattgttata tttgtcatgg aaatattaag gatttaaaca tcttttatca acgtttaaac
    14641 cattttcgtt tgatcaacgc cgtaatcaga tgaacacgcc aagaaatttt ttgccgtaat
    14701 ttagaatgtg tttcaatctc actcgcgttt aaatcatgtc ttcggtacag agttaacggt
    14761 tctcttagga acccagtctt ctttcctccc agcaaaccaa tccacatatc atgcatcggc
    14821 acttctgacg ggatcggcaa aatatcgggt tttaaagaag accggaaagc cataccagca
    14881 ccaatatagt gacttcttat cacattatta agccatcctt ctttggaatt tcgaaacgaa
    14941 aaatacgaat catggatctc ttgcagctcg ctgtccacaa tgattaaatc agaaactact
    15001 actagctgat ccggattctg ctcaaaatag tcagtgatct ttttcacttt tgtctccaac
    15061 caaacatcat cttggtctgc caaaaagatt agatctcctt tcgtctgctg gatcgcataa
    15121 ccaaaattag cgatcaatcc ttgacgaggc cccgaaaaaa gctggattcg cttatctttt
    15181 tctgcgtatt cagttaatat atcccatgta tcatctactg acccatcatc agaaatgact
    15241 acttcatctt ctgctgaaag ctgagataaa atactatcta actgctctct taaatatctg
    15301 cctccattaa aggtagctat acaaaccgaa cgcacgaatt cacaccttcc aattcttaac
    15361 atatgagcgc aaagtcatcc gccagatatg ccggtttttg acggtcaaga actgtttagc
    15421 tgttcttaac aataattgtc gtcggtgact ggataaatgc ctttgatcat attcatgata
    15481 aaatcgattt tctgcttgta aaatagaacg gtatcgatta cttgaaactt tttcatagtc
    15541 taaaaccgaa agatcatgtt ccattcgtac aggaaggatc tccacagttt tttccaactg
    15601 atccactttc caaaacagcc aatgatccaa aaaatctaaa gaaaaagcag gattaaaccc
    15661 gccaatcttt tttagaaatt tgacagacca ggcaacacct gaattgattg ccatgattcg
    15721 ttcagctgtt gggccttcaa cttctactgc ttgagctgta cgattgatat aacgatctga
    15781 atatagggga gaaacttgtc ggctcccaga aaaaatcatc ggaactgcag caacacattc
    15841 atctgtaaag gttactttac gcaatagttc aaaataatcg tcgactagcc tcgtatcctg
    15901 atccagagtg acgagacaac gtgttccttc atctgcttga gacaacgcaa aattataagc
    15961 caccgctaaa ccaggatttt gcggatcatg atgataaacg atattttctt tttcaaacag
    16021 ctcattttgc tgcctttgag gactattatc gtaaataata agctgaattt gcttttcgct
    16081 aagtgctttg cttaatggtc cataagctgg agattgactg aatagttgct gataaagcac
    16141 aacgactact aatatttctg gtttcatcag aaaaaacgcc acccccactt attgaaaaat
    16201 ttcatcatag aagtcaaaaa cacgcgaaac agttttgcat ttcgatgcgc acccttcccg
    16261 taaaaatgga taactgcata aaagggtgtg tacaataact tgtaacctgc tttttcaaaa
    16321 ctcaaacaca agtcgttgtc ttcaaagtac atgaagaaac gctcatcaaa cccctttatc
    16381 tctttaaatt tatcaatatc agctaccata aaactacctg agatcatccg tacataacta
    16441 ttagcatcag taggcaatcc tcggcattca aacacagcca gtcgtttgtc aaactttttt
    16501 ttgacccatt tgaaaggcac aaagcgcaaa acgtaatcaa aaacagccag ctgctcacgg
    16561 atgaggtgct gagttgttcc atcttcatta agaatcttag gtgctaacat cgcacattcc
    16621 ggatgttctt tcaataacaa gaccaatgct gtaactgtct cttcatccac taaaatatct
    16681 gggttacaaa taatgccata gcgagaatcg ctagctatca gattgtgatt atgtccataa
    16741 ccaaatccgg aattttcatc atgaaaatag atatcgatgt agggatattt cctatactct
    16801 gttagtcttt ttttgtactc gggaagtgaa ttgttgtcaa aaattcggat ttttactgct
    16861 gtttcatctt ttacagttga aataaaatta tctagtgtcc gaaaaatttt atcactgttt
    16921 gccgtcacaa tactaataag gatcggctgg tttaatttgt tattcatcta tatgacggtt
    16981 ccatttcttc aaatatttct gttttacttg cgtacggtac tctttattcc ctaaaaatcg
    17041 caatgttttt aaaataaact gatgtttctc attgatcaga ccaatcaatt caaataataa
    17101 aattgcagca aacaacatgg caataatcaa tatgatcgtt ccgattcttc cagtataatt
    17161 catgaccata gctgtgaaag aaaacatcaa agccatcgca taaatcgtca ggacagcacc
    17221 tttatgagta aagcctaaac tgattagtcg gtgatgaaga tgttttttat ctgctgtaga
    17281 aaaagaaaca cggtttgctt ttctcctaat gatagcaaaa aacgtgtctg ttaccggcac
    17341 tcccatgacg atcagcaaag aaatcgatga aacaaaagtc acgttcttca gcccttgtag
    17401 tgaaaggacg gccatcataa accctaggaa tagagctcct gtatcgccta aataaatctt
    17461 tgccggataa aaattgtaag gaaaaaaacc gataatactt gctacaagac agaaaataac
    17521 aactggaata tacactgtag aagcatggag gaaaaaatat ccaatgattc ctatcgtaat
    17581 taaaccgatc aatgaaatcc ctgccgccag cccatccaaa ccgtcaatca gattgaccgc
    17641 attcgtgata ccgaagatcc aaaaaatcgt tacgggaaaa ctaagccagc ctagttggat
    17701 atatccaata aaaggaagcg tgaaggaatc aatacgtata tctgctacaa aataaataat
    17761 aagagaagca atcaagatcc ctatactttt ttgtttaggt gtcagctcat atttatcatc
    17821 gagcactcct gtgactatga ttactaaaga agcaatcaag atcggtacaa tatacgattg
    17881 agggataatt ttacggaata agaataaagt cgatataaca aatgaaataa agataggcag
    17941 tccgcccgct gaaggcattg gaactttatt tattcttctt tcatttggtt tgtcgaccat
    18001 acctgtttgt actgaaatga ttttgaatac aggtgttaat atcagtgata ataaaattgt
    18061 cagaaaaaat cttataacca tactaaaaga aacaagcata tgggcaccta ctttaattaa
    18121 gagtcataat tattataaat tatatatgtc tttttgacta tctttttata tcttctcaaa
    18181 tgtccgcaaa gccttatttt atcggctcta cgggcatttt gcttttgtgg taaacctcgc
    18241 atatctaggt ctatcttctt atattttccc tgtcaatcgt ggttaaaatc gtggtaaata
    18301 cttctatgcg attagacgct gaacctctga ttttgcggta tctatggacg catgagcgta
    18361 ccagttcatt gtgatactga tgtttgaatg ccccatgata tactgtaaat ctttcgggtt
    18421 catgttcttg cttgccagcc ttgtgcagaa tgtatggcgt agcgtatgcg gtgtaatatg
    18481 tggcaaggga ttgtccttgt ggtgcttgtt gtatttcttt accatacgga taaataaggc
    18541 gttataatca atggcaactt ttggattacc tttcggattg ataaacacaa aaccgctgtg
    18601 tccgtctacc tctatcgact ttatctttgg tcggctgttg ataacccgtt gaaatgcctg
    18661 tatcgtttct ttgcttaatg gcacttgcct tatcccgctt ttcgtcttag gcgtttcaat
    18721 ataatagccc tgttccttac tctttagtaa ctggtggtcg ataatcacaa cttcattctt
    18781 gaaatcaatg tcggctactg tcagtccgca cagttccgag atacgaagtc ctgtctttaa
    18841 cagtatcagc acatcatcat aatgcttgtg atacacgttg tccgtcttaa taaatgacaa
    18901 taaggcttgc tcctgttcct ctgtcaatgc gactttctct ttggtatcat tttctatgac
    18961 ttcacttagc ttgaaatcaa aagggttttt cctcacataa tcatcttgta tggctatgta
    19021 gaatgacgct tttaacgagc gtttatggtt gttaatggta ttgtaggaaa agcctttgtc
    19081 tttcatgcgt aacgcccatt ctttagcgtc agagggtttt atcgtatcaa tgctcctagc
    19141 acctaacttg tcctctttta ataaccgcat gagttgttcc cgttgtttct gtgtgctttt
    19201 cttcacattt gtcctctgtg cgttctgttt ggcatagagt tggcaaagcg tcattttctt
    19261 gcctgtgctg tcgataccgt cctcaatatc ccgtcttatc tgctgttcca gttctcgaag
    19321 tgagggtttt tcccgttttc cctttggtgt cgggtctgtg ggtgtcaatc tccaagcata
    19381 cacatatttt gtgtttccca atgcgtctac atatttatat aagtattttc cgtctgttcg
    19441 ttggctctct ccagtatgca ggatacgacc tttgttatcc cgtctttttt ctttcatggt
    19501 gtctgctcct ttccttgttg gaaagagcct tgatatgact tgtgttcatc ataacacata
    19561 caaggctcat ttgcattaga ttgcgtccaa tttatcaata accttttcaa actgtcggcg
    19621 tttaatctgt atgcggttgc cattcataat gagccagcca gcgtccttgt tttcctctgc
    19681 caatcgtctt aacttgtttt ctccaatgcg gaaatacttt gacgcttctt caatggtaag
    19741 ggtgtatttt tcccatacgg gaatatcctg tgtattactc ataaaacacc ccctcacttt
    19801 cgattacgtc tgatatgtcc gtacacatct gttccacaag catttcctgt ttttcagaaa
    19861 ggtgggtaca ttcatcaatc attttcatgt agtttgaacc tgtcagcctg tcgatttcct
    19921 ctatcatcac gattgacggc atgacctgtc tttccagcca ttgcagagtg cgttccatat
    19981 tgacaggtac agggttcatt tcaaaaggta aactgtcatg ggaaatgaat atctcccaca
    20041 atggatagtc aggaaattcc acaaaatcgg taatgagata aaacaccagc ccatgagggt
    20101 tatacgtcaa taacagttct tcaactgcct gtaccgcctt tttgtcacgc agacgaattt
    20161 caaaacggtt gataatatcg gtatgtttct ttttcgttgc ctgttccttt tgtttttcat
    20221 acaggcagaa atattttgtg cttgcctttg agccgatata aagggtactt gctaaattac
    20281 ggttttttcc gcttaatttt ccaccttgta cattttcata gtttttacag cgacattccg
    20341 caccaccatt tctgtatttt tcagatagca caggaatatc cagcagaccg cacatatcat
    20401 tgattgccaa gtcaaaacgt cggataacac cgccacagtc aagacagcgg tttaagaatg
    20461 aataccagtc cctgttctgt gcctgtaaga catattccat gttgcgtgag cctgcccctt
    20521 ttaattctaa aaatacgccc atgtgttcat catcagaagc catgaccttg atttccccgt
    20581 aagcataatg ctccttatag ccataatacc catagtcata atggataaaa taatctgctt
    20641 tcatgccaag cacctttcgg ataacttcca atgcgtcagt cgttggaaaa cggacagaca
    20701 gataatcaac gagcagataa aaaggcttat cgcagatata gtaattcaga caacgcagga
    20761 taatgttctg tgtttccttg ctggcgttag cttttccgtt ctcaaagcgg ttgatactat
    20821 atcttgatac atgggtaaga cttgcaagtt ctccttgtga aagaccgctg gcaagacgtt
    20881 tgttcttcat ttcaagtgca aattctaaat tgttcattgt attcctccaa tcccacaata
    20941 gccttgaaat acctgtaaac cacctttcaa aaagtgtgat atgtactatc ttcttaaaac
    21001 ctctcattac tactcacagg tttgggggtg tatggttttt gacttgtctt ttgtgaaata
    21061 gtgcccccct gttagataac gggggcacag acaaggctcg caggctcgcc agccgacaaa
    21121 gcatttcttc cccttgacac aaggtgtcat ggcggtaagc ctgagaggct tccgccaccg
    21181 tgtcagaaat actttgtcag caagttctgt tttgcctttt ctaacaagtt ccatttcaac
    21241 cgatattcat atctacggat ttaatcagat aggtatttct ttttgaatgt ttttatattt
    21301 cattctattg tgcttattcg attacaagaa catagtaatt cttatatgct tatttgtcaa
    21361 tatctttttt tgcctttaca aatcatatta agcatgatag acttataata gcgaagatat
    21421 aacgagatat ggagggttat gcaatgaaaa taagccattt aggaaataat atacagacca
    21481 taagaaaatt taggggaatg aaacaacagg aacttgcgga caaaatcggt atcaatatgc
    21541 agagcctttc caagattgaa agagggttga actatcctgc ctatgaaacg ctggaaaaga
    21601 taatggaagt gctggacgtt acgcccaatg aattgttgtc gggagaatgg aagtatgtca
    21661 atcaatctga aaaggaagtc tgtcagtttt taagagccga agaacgtcta aatgcagaac
    21721 tgaaacatgg acactatgat aacttctttg acagcgagga agaatggctg gaatatgagt
    21781 tagaaaagtt acgggaatat attacagact atatcaacgg gaaaagcatt gaagcgtccg
    21841 acctttaccc aatcaaagaa tttatccagc atttgaagtt tcaaaaatta ttagaccgct
    21901 atgatgattt atacagtatg gatatgtttg gggaaagcat agagggacat aagtataaga
    21961 caccttatca agtcgtgaaa atgataaacc cgaactcaaa agaagatatg gaactgttac
    22021 gagaagtatt aaggaataac aattttgatg atgaggacga gtaacttttt tcctcttgcg
    22081 agaagataca gcaaacaaaa agcccgatca agagtgcaaa gcaccaccaa tggtggcaaa
    22141 gctcttgaca gggctttttc ttttctgttg tgggcaatca agaggaagaa agggataaaa
    22201 tctgatttgt ggtaaatcag gcaaaggcaa cgcaggtatc acagtatgaa ttgacaccgc
    22261 ataaggaaag caactggaag attgtagaat aaatgaaaaa ggcttgcatt ctgcattttt
    22321 ataaaattgc aagtctttat attgacaagc ataaaatact aagatatact ttaatcagac
    22381 caacggtttg tattggaggt aagttatggc acgaaataaa tatccagaag aaacagtaaa
    22441 actcatttta gatgtttcta caaaaatgtt ttgtgaaaaa ggatatgata acacatcatt
    22501 gcaagatatt atcaatgaaa caaaactttc taaaggtgct atttatcatc atttcgattc
    22561 aaaggaagat attttaaaag caatttttca aagaattgga aatgaaaatg cagtaatatt
    22621 tgctaaaatt agagataacg atagtttaaa cggtttagaa aagttaagag aaatatttaa
    22681 gacagcggta ttcaattcta accaaagcgt tttattgaca gtttctcctc aactattaaa
    22741 caatcctcgt tttcttgcta tgcaaataga acaaatgtat gaaattgtag tacctgaatt
    22801 tgttgaaccc gttttagaac aaggtatcaa ggataagtct ttggagattg aaaattcaca
    22861 tgaaattgca gaagttatca tggtattaag taatgtatgg ctcaacccat tagttgaaat
    22921 gacggataaa gagggtatgg aaaaacgttg caatacattc aatgcccttt taaatggaat
    22981 aggcatagac acattattag acgaagaaac aatttctgca tttatacagt tttgtaaata
    23041 actgtatttg ttatccctat acgatagact gctctataaa agagcagttt atttaaacca
    23101 ttatataaac cgacggtctg tttgattaaa taaaaaggag agattttatg caagcagaaa
    23161 aaaaactttt taatcgtgat tttatacttg ttgttatagg acagataatt tctttatttg
    23221 gaaacgctat cctacgattt gcattaccgt actatttgtt aaaagaaact ggttcatcag
    23281 ctttgtttgg tattgctaca gcctgctctt ttttacctat ggttattttg tcgctggtag
    23341 gtggtattat tgctgacagg acaaataaaa gaaacattat ggtcatacta gattttgtaa
    23401 cagcaatcat aattttagta ttttatttgt cattgggaaa aattcctatc atacctttgt
    23461 ttattgttgc tttgatgtta ctttatggta tttctggtgc atatcaacca gcggtacaag
    23521 caagtattcc tctattggta tcatcagata aacttaccgt cggtaatgct ataataaatc
    23581 aagtaaatac tttagcaaac
//
LOCUS       DANKKG010000033        23568 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000033 DANKKG010000000
VERSION     DANKKG010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.033"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..1119
                     /gene="dnaN"
                     /locus_tag="PW004_001085"
     CDS             <1..1119
                     /gene="dnaN"
                     /locus_tag="PW004_001085"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HDL2726073.1"
                     /translation="LNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTLTGSN
                     ADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQVAIT
                     SGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPILTGV
                     HFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEEIVEI
                     SIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERASLLS
                     HEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKAALRA
                     FGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            1348..1590
                     /gene="yaaA"
                     /locus_tag="PW004_001086"
     CDS             1348..1590
                     /gene="yaaA"
                     /locus_tag="PW004_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HDL2726074.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            1577..2701
                     /gene="recF"
                     /locus_tag="PW004_001087"
     CDS             1577..2701
                     /gene="recF"
                     /locus_tag="PW004_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HDL2726075.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDSRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            2698..4644
                     /gene="gyrB"
                     /locus_tag="PW004_001088"
     CDS             2698..4644
                     /gene="gyrB"
                     /locus_tag="PW004_001088"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HDL2726076.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            4664..7135
                     /gene="gyrA"
                     /locus_tag="PW004_001089"
     CDS             4664..7135
                     /gene="gyrA"
                     /locus_tag="PW004_001089"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HDL2726077.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            7371..7670
                     /locus_tag="PW004_001090"
     CDS             7371..7670
                     /locus_tag="PW004_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HDL2726078.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            7720..8232
                     /gene="ssb"
                     /locus_tag="PW004_001091"
     CDS             7720..8232
                     /gene="ssb"
                     /locus_tag="PW004_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL2726079.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            8258..8494
                     /locus_tag="PW004_001092"
     CDS             8258..8494
                     /locus_tag="PW004_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HDL2726080.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            8637..10610
                     /locus_tag="PW004_001093"
     CDS             8637..10610
                     /locus_tag="PW004_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HDL2726081.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            10616..11068
                     /gene="rplI"
                     /locus_tag="PW004_001094"
     CDS             10616..11068
                     /gene="rplI"
                     /locus_tag="PW004_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HDL2726082.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            11342..12709
                     /gene="dnaB"
                     /locus_tag="PW004_001095"
     CDS             11342..12709
                     /gene="dnaB"
                     /locus_tag="PW004_001095"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDL2726083.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            13489..14781
                     /locus_tag="PW004_001096"
     CDS             13489..14781
                     /locus_tag="PW004_001096"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HDL2726084.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
     gene            15568..16431
                     /locus_tag="PW004_001097"
     CDS             15568..16431
                     /locus_tag="PW004_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDL2726085.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(16560..18182)
                     /locus_tag="PW004_001098"
     CDS             complement(16560..18182)
                     /locus_tag="PW004_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2726086.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            18431..18952
                     /locus_tag="PW004_001099"
     CDS             18431..18952
                     /locus_tag="PW004_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HDL2726087.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(19138..20868)
                     /gene="spxB"
                     /locus_tag="PW004_001100"
     CDS             complement(19138..20868)
                     /gene="spxB"
                     /locus_tag="PW004_001100"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HDL2726088.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            21033..21851
                     /locus_tag="PW004_001101"
     CDS             21033..21851
                     /locus_tag="PW004_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HDL2726089.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKHHFAVFGVMSGFIIMMILDVALG"
     gene            complement(21954..22151)
                     /locus_tag="PW004_001102"
     CDS             complement(21954..22151)
                     /locus_tag="PW004_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726090.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            22320..22898
                     /locus_tag="PW004_001103"
     CDS             22320..22898
                     /locus_tag="PW004_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HDL2726091.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            22993..23130
                     /locus_tag="PW004_001104"
     CDS             22993..23130
                     /locus_tag="PW004_001104"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726092.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         7570 a         4239 c         4924 g         6835 t
ORIGIN      
        1 ttaaaccgag ctagctttat gcaggaattg caaactgttc aacgagctat ttcaagcaaa
       61 accacgatcc ctattttgac aggtgtaaaa atcacactga cacaagaagg tttgactttg
      121 acggggagca acgctgatat atcaattgaa acttttttgt ctgttgaaaa cgaaaaagca
      181 aatatgcaaa tcgaatctac tggttccatt gttttacaag cacgtttctt tagcgaaatc
      241 attcggagac ttcctgaaga aacatttact ttagaagttt tagaaaataa acaagtagcg
      301 attacttctg gaaaagcgaa ttttatcgta aatggattag atgcagataa ctatcctcat
      361 cttcctgttg tcgaaagcca taaccagatg aaattacctg tacacgtatt gactaaacta
      421 atcaacgaaa cagtttttgc tgtctcacaa catgagagtc gtccaatctt gacaggtgtg
      481 catttcattt tatctgataa ttctttattg gctgtagcta ctgattctca ccgtctaagt
      541 caacgcgtga ttccagtaga acaagcggct gatcattttg atattgttat tcctggaaaa
      601 agtttgatcg aattatctcg ctcattaaca aatgaagaag aaatcgtcga aatcagcatt
      661 atggaaaacc aagtgttgtt caaaacagaa acgatgtatt tctattcccg tttgctagaa
      721 ggaaactatc ctgataccaa tcgtttgatt ccttcaagtt ttaatacaga ggttgaattt
      781 tctgttccta gctttttagc ggcgatcgaa cgggcttctt tactttctca tgaaggccgt
      841 aacaacatcg ttcgtttatc tattcgtcca gatgcggttg ttttatacgg aaattcgcca
      901 gaaatcggga aagtagaaga aagcttgagt tatacagcaa gcagtggcga tccattagat
      961 atttctttca atccggatta tatgaaagca gcgttgcgtg catttggaga tatgagcatc
     1021 aaagtgaaat ttatctctgc tattcgtcca tttacattgg aaccaacgga agatggcgtt
     1081 caatttattc agctgattac tcctgtacgt acaaattaac aatgattttc attgttcttg
     1141 aaaatttaat tgaaaagaaa tagaaaaaat aaaattttaa acagaaggcc ctaaaatctc
     1201 ttttaaggct ttctgtttat ttttgtttaa atcacctttt ttagattaaa tcgtcttata
     1261 tggaaaaata tatggataaa tttgttttat tgcgaaaaaa aaggtataat agagtatact
     1321 tttaataatg aaaatgaggg atgggcattg aaacaacaag tcattctaaa tacggaattc
     1381 atgacgttgg gccaaatgtt gaaagaagtc acagttatcg gtagtggcgg acaagcaaaa
     1441 tggtatttgg cagaaaacac tgtgttagtc gatggcgaac cagaaaatcg tcgtggacgc
     1501 aaactttatc ccggcatgat ggttgaagtt ccagaagtag gaactttttt tatggtaaaa
     1561 aaaggaaacg acgataatga ggctgaataa gctgtattta aaaaattatc gaaattacga
     1621 agagctgaac atcgaatttt ccaagaacct cgtcatcttt ttaggagaaa atgcgcaagg
     1681 aaaaacgaat cttttggaaa gtatctacgt attagctatg acgcgaagtc atcggacaag
     1741 tagtgagcag gaattgattg gctggactga cgatcaggcg atgattcaag gagagatcac
     1801 aaaaggttca tccactcttc cattagaaat cttattatct aaaaaaggcc gaaaaaccaa
     1861 gatcaaccat atcgaacaaa aaaagttaag tacttacgtg ggacaaatga atgtcatttt
     1921 atttgcacca gaagatcttt ctttagtaaa aggcagcccg cagatcaggc ggaagttttt
     1981 agatatggaa ttaggccaga tcaatccaat ctatctttat gatcttgtcc aataccaagc
     2041 aattttgaaa caaagaaacc agtatttaaa gcaattgaat gaaaaaaaac aaactgattt
     2101 gctttatttg gatattttgt ccgaacaact agcagcgttt ggcagcaaag tattgaaagc
     2161 aagagcacag ttcgtcaagc gattggagtt ttgggctaat tctttgcatc agcaaatcac
     2221 ccatcaaaaa gagcagctgg aaatcgagta tctcaccgca gtcgattctt tagaaacaca
     2281 tacgcaagag caaatccaag aacaatttct cgcacttttg aaccaaaata aaaagaaaga
     2341 cctttttcgg ggaacaacga cagttggtcc tcatcgtgac gatctttcct tttttattaa
     2401 tcagaaaaat gtgcagactt atggatcgca aggacagcaa agaacaactg cactaagtgt
     2461 caaattagca gaaatcgatt tgattaaaga agagacgggg gaatatccaa tccttttgtt
     2521 agatgatgtg atgagcgagc tggatgatag tcgtcaactc catctgctag aaacaatcga
     2581 aggaaaagtt caaacgttct taacaacaac gacacttgac catgtaaaaa ataaaatgac
     2641 tgttgaacct gagatttttt atgttcaaca agggaaacta aaaggagaag aaactgaatg
     2701 acagaagaaa gaagtttagt agaacgcgct aaagagtatg atgccagtca gattcaagta
     2761 cttgaaggac ttgaagccgt tcgtaaacga cctggcatgt atatcggttc gactagttct
     2821 gagggtctgc atcaccttgt ttgggaaatt gtcgataatt cgatcgatga agtattagca
     2881 ggttttgcaa cgaaaatcca tgtgattatt gaaaaagaca acagtatcac cgttattgac
     2941 gacggtcggg ggatacctgt agatatccag gcaaaaactg gacgtcctgc agttgaaacg
     3001 gtctttactg ttttacatgc tggaggaaaa ttcggcggtg gtgggtacaa agtttctggt
     3061 ggacttcacg gagtaggatc ttctgttgtt aacgcgcttt ctacacagct tgatgtcaaa
     3121 gtatacaaag aaggaaatgt gtactatcaa gaatatcgtc gtggagctgt agtggacgac
     3181 ttaaagatta tcgaacaaac ggatcgccat ggaacaacgg ttcactttac gccagaccct
     3241 gaaattttta cagagacaac tgagtttgat ttttcaaaat tggctacccg gatccgtgaa
     3301 cttgctttct tgaaccgcgg gatgcgtata tcgattgaag acaaacgaga agaagaacca
     3361 gtcatcaatg aataccatta tgacggcggg attaaaagtt atgtcgaata cttgaatgca
     3421 aacaaaaccg tgatttttcc agaacctgta tatttggaag gtgaacagca agatatcgca
     3481 gttgaagtct ctatgcaata tacagacggc taccattcca atatcatgag ttttgccaac
     3541 aatatccata cgtatgaagg cggaacacat gaatcaggat tcaagactgc tttaactcgt
     3601 gtgataaatg attatgcccg aaaacaaaag ctgatgaaag aaaatgacga caacttgacc
     3661 ggtgaagacg tgcgagaagg attgacagct gttatttcta tcaagcatcc tgaccctcaa
     3721 ttcgaaggac aaacaaaaac aaaattgggc aattccgaag tacggacagt cactgatcgg
     3781 ttatttagcg aacatttcat gaaattcttg ctggaaaacc caagtgtggg ccgtcaaatt
     3841 gttgaaaaag gattactagc ttccagagct cgtctagcag cgaaacgtgc gcgtgaagta
     3901 acgagacgta aaggtgcttt agagattagt aatctgccag gaaaattagc ggactgctct
     3961 agcaacgatc ctgaaaaatg cgaattgttc atcgtcgaag gagattcggc cggcggatca
     4021 gctaagcaag gtcgtaatcg agagtttcaa gccatcttgc ctattcgtgg gaaaatcttg
     4081 aatgttgaaa aagcaagcat ggacaagatc ttggcaaatg aagagatccg gtctttattt
     4141 actgctatgg gtacagggtt tggagaagat tttgatgtat cgaaagctcg ctaccacaaa
     4201 ttagttatca tgactgatgc cgatgtcgac ggcgcgcata tccgtacatt gctgctgact
     4261 cttttttatc gttacatgcg tcctattgtt gaagctggat acgtctatat tgctcagcca
     4321 cctttatacg gggtaaaaca agggaagaat attacgtatg tacaaccagg aaaaaatgca
     4381 gaagaagagt tgaaacaagt cgttgcttca cttcctgcct caccaaaacc aagcgttcag
     4441 cgttataaag gacttggaga aatggatgat catcaactat gggaaacaac tatggaccca
     4501 agcaatcgga tgatggcccg cgtcagtgtg gacgatgcta ttgcagcaga tcagatattt
     4561 gaaatgttga tgggggatcg agtagaaccc cgtcgtgcat tcatcgagga aaatgcacac
     4621 tatgtgaaga acttagatat ttaatcatga aggaggaaaa agaatgagtg aagaaatcag
     4681 agaaaacatc catgatgtca atctaaccag cgaaatgaaa gactccttca tcgattatgc
     4741 gatgagtgtt attgtcgcac gtgcacttcc tgatgtcaga gatggtctga aacccgttca
     4801 tcgccgtatt ttatacggca tgaacgaatt aggtgtaaca ccggataaag cccataaaaa
     4861 atctgccaga atcgtcggag atgtcatggg taaataccat cctcatggag actatgcgat
     4921 ctatgagtca atggtacgga tggcacagcc attcagctac cgttatatgc tagtggatgg
     4981 tcatggaaac tttggttctg ttgacggtga cggtgccgcc gctatgcgtt ataccgaagc
     5041 acggatgagc aaaatcgcta cagaaatgct gcgtgatatc aataagaaca ctgttgattt
     5101 ccaaagcaac tatgatgata cagaaaaaga accagttgta ctacctgctc gcttcccgaa
     5161 tcttttagtg aacggaacaa cggggatcgc cgtaggtatg gccacgaata tcccacctca
     5221 taacttgtct gaggtagtag cggctatcga tttgctgatg gataatcctg atgtgacgac
     5281 caatgaattg atggaagtat tacctggacc agattttcca acaggtggtt tggttatggg
     5341 aaaatcagga atccgaagag cgtatgaaac aggtaagggt tctattaccg tacgtgcaaa
     5401 agttgagatt actgagatgc caaatggtaa agaacggatc atcgtgacag aattgcctta
     5461 tatggtcaat aaagcaaaat tgatcgaacg gatttctgaa ctgcacagag acaagcgtat
     5521 cgaagggatc acagatttgc gggacgaatc ttcccgtgaa ggtatgcgga tcgtcattga
     5581 catccgtcgt gacgccagcg catctgttat cttgaataac ttatataaat tgacatcctt
     5641 gcaaaattca tttggattca atatgttggc gatcgaaaaa ggtgtgccga aagttttgag
     5701 cttgaaacaa attcttgaaa actatatcga acatcagcgc gaagtcatca caagacggac
     5761 agtctttgaa aaagaaaaag cagaagcaag ggcacacatt ttagaaggtt tgcgtattgc
     5821 tttggatcat atcgatgaaa tcattgcgat tatccgcggg tctaaatcag atgacgaagc
     5881 aaaagcgacg atgatcgaac ggttcgattt atccgatcgc cagtctcaag ctattttaga
     5941 tatgcgttta cgccgattga ccggtttgga acgggaaaaa atcgaaaatg aataccaaga
     6001 actcttaaaa ctgattgaag atttaactga cattctggca agaccagaac gtgttacaga
     6061 aatcatcaag aatgaattag cagacttgaa tcaacgattt ggtgataaac gacgcacaga
     6121 attactagtc ggtgaggtct taagtctgga agatgaagat ttaatcgaag aagaagaaat
     6181 cgttattaca ttgacaaaca atggttacat caaacgtttg gcaaataatg aattcagagc
     6241 tcaacgaagg ggtggacgtg gtgtccaagg aatgggtatc cacaatgacg acttcgtcaa
     6301 aaatctggtt tcttgttcta cccatgatac attgttattc tttacgaata acggaaaagt
     6361 gtatcgggca aaaggatacg aaattccaga atacgggcgc acagccaaag gaataccaat
     6421 tatcaattta ctaggtatcg attcgagtga aagcatccag gcaatcattg cggtgacggg
     6481 agaagcagaa gaaggccact atctcttctt tacgacgcgt caaggtacag taaaacgtac
     6541 ttctgtcact gcttttgcta acatccgaag taatggtctg atcgcaattg gtttgaaaga
     6601 agacgatgag ctcgtcaatg tgctactgac agatggtcaa tctaatatca ttattggaac
     6661 acataacggc tactctgtca ctttcaaaga agaagcagtc cgcgatatgg gacgtacagc
     6721 ttccggggtt agaggaatac gcctgcgtga ggaagattat gtcgttgggg cagccttaat
     6781 gaaggaagat caagaagtat tagtgatcac agaaaaaggt tacggaaaac ggacaaaagt
     6841 atcggaatat ccagtgaaag gccgcggagg taaagggatc aaaacggcga acatcactga
     6901 aaaaaatggt ccactagctg gattgaccac tgtttctgga gatgaagaca tcatgctgat
     6961 tacagataaa ggtgtcatta tccgtttcaa cgtatcgact gtttctcaaa ctggacgttc
     7021 aacattaggc gtacgtttga tgaagatgga agctgatacg aaagttgtca cgatggcagc
     7081 agtcgaacca gaagctgcag aacaaacagt tgaagaacaa ggatcagaag aatagccatc
     7141 aagactaatt gtgtaagcta gcaagaatga ccgattaaaa aatctggtta ttcctgctgg
     7201 cttttttgat tagttgaatt tctcttaaaa tattcgaaac tgttcattct gtattgcttt
     7261 tgaccaaaat agagtgtata atcagagctt gtgagtagtc taattaatta gataactctc
     7321 cttgctctta ggaacctaag agccgaaagt ccacagggag gtgaatatca atggaaaaca
     7381 cgaaatatga aatcatgtac attattcgtc caaacattga tgaagaagca aaaactgctt
     7441 taatcgaacg tttcgatgca atcttgaaag ataacggagc agaagttatc gaatcaaaag
     7501 attgggaaaa acgccgtttc gcttatgaaa tgaacggtta ccgtgaaggt atctatcaca
     7561 tcgttaaagt atcttctcca tcatcagcaa atgctgtcaa tgaatttgac cgtcttgcta
     7621 aaatcaacga cgatatcatt cgtcacatga ttgttaaaga agaagcttaa ttgttgtttc
     7681 atgtgaaaca atagaaaacg atgagaggag atgactattt tgattaataa tgttgtatta
     7741 gttggacgtc tgacgaaaga tccagatttg cgttacacag caagtggtac ggcagtcgca
     7801 acatttactt tggctgtgaa tcgtaatttt acaaaccaaa gcggcaatcg cgaggctgat
     7861 tttatcaact gtgtgatttg gcgcaaatca gcagaaactt tagcaaacta tgctcgtaaa
     7921 ggaacacttt taggtgttac tggacgtatc caaactcgtt cttatgataa tcagcaagga
     7981 caacgtgtat acgtgacaga agtagtagct gacaacttcc aattgctgga aagtcgttct
     8041 gcatctgaaa atcgccaaca aagcggcgga ttccaaagtt ctggtcaatc agctggcgga
     8101 tttggcggaa acaataactc taatcaaaca tctcaatcat ccaacggtat gcctgatttc
     8161 gaccgtgata cttctgaccc attcggctca agttcaacga tcgacatttc agacgatgat
     8221 ttaccattct aaacgaattt gataggaggg aaatagaatg gcacaacaaa gaagaggcgg
     8281 acgtaaacgt cgtaaagtag actacattgc tgctaaccat attgaatata tcgattataa
     8341 agatatcgaa ttactaaaaa gatttatttc agaacgcggt aaaatcttgc cacgtcgtgt
     8401 aactggtaca ggcgctaaaa accaacgtaa aattactgtt gcaatcaaac gcgctcgtat
     8461 catgggcttg ctacctttcg ttagcgaaga ctaataaatc ctaggaattg aatgactgat
     8521 ccttttgatc agctattcaa ttcctttttt tctgtaaaaa taacctctat tcgtttagtc
     8581 gctcgaatga cgaaagttat ggtaaaatca aattagtcat atatgggagg aaaattatgg
     8641 aaaataaaaa caatcattta tccccgaaac ctctcttcct tatcctgttt atattaatcg
     8701 aagtattggt agtcttaatt ttaccatttc gttttgttag cgcttcggtc attctattga
     8761 tgaatttatg gggtgttcgc tattggttga aaatgaaacg ttggcttatt ttaaataaac
     8821 gtgaaaaaat ccagcaggct tcgactgtag ctgaaaccaa tctagcttat gtctcagaag
     8881 tcatgcctgt aggagtgatt gcttatcagc catcaagcca aaaaatcgaa tggctcaatc
     8941 cattagcatt gaaaatcaaa gatcaaagtg gattaacaga ggaagaattg ctagatactt
     9001 tttttactgc aaaagaaaaa aagaaaaacc aagtctatct agcagataaa acatttcagt
     9061 ttgatatgga tacagtaaag ggtgttgttt attttaatga tattacccaa aaccagttgt
     9121 taaaactcca tcagacgaac agtcagccgg tgattggtat tttatctatt gataactatg
     9181 cagatgccat tgataaattg gatgacaaag agatttctta tttgaactct ttcgtcacta
     9241 cactagtttc tgactggatg aatgaatacc atgtcttttt caaaagaatc aacgcagaac
     9301 ggtttttctt cgtagcacag atgatcgatc tgaaaaagat gatggataaa aaatttgatt
     9361 tgctggatcg ctttcgagca gaagctgaaa aacagagctc gcctatcacc cttagtatgg
     9421 ggattgctta tggctcaaga accctcgatg aaatcggtgg agaagctcag aataatttag
     9481 atgttgcttt agtccgtgga ggcgaccaag ttgtcgtgaa agaggtaaaa gaaggagcaa
     9541 aacctgttta ttatgggggg aaatctgcta agaatgtcaa acgcacgcgt gttcgttctc
     9601 gagcgatgag taccgctttg cgtcggattt ttgatgaaac aggcgatgtg tttatcatgg
     9661 ggcacaagtt tccggatatg gatgctttag gctctgcttt tggtgtcagt tgtctggcac
     9721 gcttcaacca gaaaaaagct tacatcgtta tcaacgagaa cgagatcatt ccagatgtag
     9781 aacggtgtct tgaagaaatc cataagcatc ctgaattaga aatgcaacta atctctccag
     9841 aacgtgccat agagctgaaa aaagaagagg acctcctcgt gatggtggat taccataaac
     9901 cttctctatc gatctctcag ggcctatatg agcaatttga taagattgtg atcattgatc
     9961 accatcgtcg tggggaagag tttccaagta aacctctgct gacttatatc gaatcttcag
    10021 cttcttcagc ttcagagcta gtttctgagc tgatccaata ccaaagcagc aaaacaaatc
    10081 gcttagatcg ttttgaggcc acattattgc ttgcagggat ggttgtagat acaaatagtt
    10141 tcaatgtccg tacaactgca cgaacttttg acgttgccag ctatttaagg tcttgtggag
    10201 cagacgcatc cttagtacaa tatttattga gctcggattt agcgacatac ttggagatca
    10261 gcaatttgat tgctggaagc gactatgtga cttctgatat tgttgtggcg gcagggacag
    10321 acgaaaaaga gtataatagt gttatagcag caaaaacagc agatacattg ctttcgatga
    10381 tcaatatcaa tgcagctttc gtcattacaa agcggacaga cggcttgatt gggatcagtg
    10441 ccagaagtac cggcacgatc aacgtccaaa tcatcatgga atccttaggt ggcggaggac
    10501 acttcacaaa cgcagctact caactggaaa atacgacagt tgaggaaaca aaacagaagc
    10561 tatttgctgc aatcaaagaa aatatgaatc aaatatatga taaggagtaa tccaaatgaa
    10621 agtaattttt ttacaagatg taaaaggaaa aggtaaaaaa ggggacatca aagaagttcc
    10681 agcaggttat gcacaaaatt atctaatcaa aaataacttg gcaaaagaag caacgaaaag
    10741 cagtatcagt cagttagcag gacaaaagaa agcacaagaa aaacacgaag ctgaaatctt
    10801 ggaagaagct aaaaagttaa aagaattctt ggaaaaagaa gatactgtag tcgaattgaa
    10861 ggcgaaagca ggggaagaca gccgtttgtt tggttcgatc ccttcaaaac aaattgctga
    10921 agggttaaac aaacaataca aaatcaaatt agataaacga aaaatcgaat tagaaaatcc
    10981 gatccgttca ctaggctaca caaacgtacc tgttaagctt catcatgagg tgacggctac
    11041 gatcaaagtt catgtcgtac aagaataaca aatcttgata gcgatgttca gctggtaagt
    11101 ataaccagaa ggaaaaaaga caaaccgaaa tttatctgag aaaacagctg cagcaatgaa
    11161 agcaaaagct gcaaatcgat ttcagattga tttcggtttc ttttttggga acagcaaaat
    11221 agcataaaga aaacgagtat aataaaagag aactattttt ttattacact ttcggtacga
    11281 atgatgactt ttctctcatt ttcttgtaaa atgaagcaat tgaagacaag gagcgatacg
    11341 tatgaatgaa gtgtggcagg atcgaatccc accgcaaaat attgaagctg aacaagcggt
    11401 tttaggctct gtttttcttg acgcagaaac gatcattgat gccatggagt acatagagcc
    11461 aaaagacttt tatcgtcgtg gccatcaaat tatttttgag acaatgatcg agttaaatga
    11521 tcgtaatgaa gcgattgacg tggttactgt caaagaccgg ctggaacaag ccaatttgtt
    11581 agaagatgcc ggtggtttaa gttatctctc agacttggca ttagcagttc cgacagcagc
    11641 aaatatcgtc tactatgcga aaatcgttga agaaaaatca ttgctgcgta cattgatcca
    11701 gacagcaaca ggaatcgtta cgaaaggatt cgagcaagga gaagaagttc aaacaatttt
    11761 agatgatgcc gaacgcagta tcttggaggt ttcggaaaaa agaaatcgaa gcggcttttt
    11821 atctattgct gaagtactga actccacgat tgaaaatatc gatcgactag ctcaaaacaa
    11881 tgaagaaatc acaggtctac caactggcta tcaaggtctg gataaaatga cagcaggttt
    11941 ccagcccgaa gaactaatca ttcttgctgc acgtccagcg gtaggtaaaa cagcgtttgc
    12001 attgaatatt gctcaaaatg taggaacaaa aacagatcgt tctgttgcga tatttagttt
    12061 ggaaatgggg gcagaatctc ttgtaaatcg gatgctttgt gcagaaggtt cgattgaggc
    12121 cagccattta cgtacaggtc agctttcgga agaagaatgg tcgaatctga tcgttgcgat
    12181 ggggagtctt tctcgagcaa atatttttat cgatgataca ccagggatca aaatctctga
    12241 aatccgtgcg aaatgtcgga aactagcgca agaaaaaggc aatctaggct tgatattgat
    12301 cgattatttg cagttgattg aaggtaacgg taaagagaac cgtcaacaag aagtatcaga
    12361 tatctctcgc caactgaaaa aactggcgaa agagctgaaa gtacctgtca ttgctctttc
    12421 acagttatct cgtggagtag aacagcgaca agacaaacga ccagtgctaa gtgatattcg
    12481 tgaatccgga tcaattgaac aagatgcgga catcgtagcc ttcttgtatc gagatgatta
    12541 ttatgaaaga gaaggcggag atgacgatga tggaaacgag ccaaaaaatg acaacgtcat
    12601 cgaagtcatc atcgagaaga accgaagcgg tgcacgagga acagttgaac tactgtttat
    12661 caaagaatac aataagtttt cgtcgctttc gccaagagaa gaattttaaa gaagaggttg
    12721 tgagccttga gcaatcccga gagctctcgt aagaagatgc aaggagtaac cgcaggaacg
    12781 actagagtac ttaatttaaa cagatctcca aagggattgt gacaaaagtc ttgtcacagt
    12841 ccctttttcc gaataaacgg tgttcaatca gctgaccctt ggtattagct cagcaaacgt
    12901 caaaatatga ggaactattt tgacgtttgt ttctctaata ctcaggtcaa aacgctgatc
    12961 ttacgacctc ttttccttaa acggtgttct cctgccaact cctcggtcac aagtcacaat
    13021 atcatcaaac atgagaagca gttttttgaa attgctcctt gtgactcaga gttaaacggc
    13081 aggctcacaa cctctttctt aaacggtgtt caaaagcttg catctgcggt aatacgccca
    13141 atcaaacgaa aaatatgaga agctattttt gtttggctgt tcttattact tgatgcagga
    13201 cagctttttc acaacctctg attcacggtg ttccaaaagt agcttcttcg aaaaataagt
    13261 cgaaatttca caaaaatttg agaagcaatt ttcgtacatt ccttcttatt tcttgaagct
    13321 gaccacttct gttacaacct cttttttaat aaccattcta aaaagataaa gttcgttttt
    13381 taagcgtttt tataaaataa aaataaaaat cattcgattt tatttgaaat atgtatctat
    13441 tattgataca atggttcagg attaaataat atagaacgag gtgttcacat gtcatctgta
    13501 gtagtagtag gtacgcaatg gggcgatgaa gggaaaggaa aaatcacaga ctttctcagt
    13561 gaaaatgctg aagtgattgc acggtaccaa ggtggcgaca atgcagggca cacaattaag
    13621 tttaacggtg tgacttataa actgcatttg attccttctg ggatttttta taaagacaag
    13681 atcagtgtga tcggaaacgg cgttgtagtc aatcctaaat cattggttaa agaattagct
    13741 tatttacatg aaaaaggtgt agaaacaagt aatttgcgta tttctgaccg cgcgcacgta
    13801 atcttgcctt atcatatcaa attggaccaa ttacaagaag atgctaaggg cgaaaataag
    13861 attggtacaa cgattaaagg aatcggacct gcctatatgg ataaagcagc tcgtgtaggt
    13921 atccgtattg cagacttatt agataaagaa atttttgaag aacgtcttcg tttgaatcta
    13981 gatgacaaaa accgtcaatt tgtaaaaatg tttgattcag aaccacttga ttttgacgat
    14041 atttttgaag aatattatga atacggacaa caaatcaaac aatacgtcac agatacttcc
    14101 gttatcttga atgatgcttt ggatgcaggc aaacgcgtgt tgtttgaagg cgcacaaggt
    14161 gtaatgctgg atatcgacca gggaacttat ccttttgtta cttcttctaa cccagtagcc
    14221 ggtggggtaa caatcggaag cggtgtaggg ccttcaaaaa tcaataaagt tgtcggcgtc
    14281 tgcaaagcct atacgtcacg tgtcggtgat ggaccatttc caactgaatt atttgatgaa
    14341 acaggagaac aaatccgcga agtaggacga gaatacggta cgacaactgg tcgtccgcgt
    14401 cgtgtaggct ggtttgattc tgtagttatg cgccattcta aacgagtttc aggtattacc
    14461 aacctatctt taaactcgat cgatgtatta agtggattgg aaacagtaaa aatttgtacg
    14521 gcttatgaat tagatggcga actaatctac cactatccag caagcttgaa agagttgaac
    14581 cgctgcaaac caatctatga agaattgcca ggctggtcag aagacattac aggatgcaaa
    14641 acacttgcag aactgccaga aaacgcacgt aactatgtac gtcgtatctc agagcttgtt
    14701 ggtgtacgta tctcaacatt ttctgtcgga cctgatagaa cacaaacaaa catcttagaa
    14761 agcgtctggg cgcaaatcta aggataaaaa cgacgactga atcaggacgt tcttaccaga
    14821 gcgagtcgaa gtgtttatct atacgataat aataacttga agaggtagtg aaagagacgt
    14881 ttagccttat tgccgatagg ctgtctcttg aacactgttt attcagaata agggactgtg
    14941 acaaaaaccg tgtcacagtc cctttttccg aacaaacggt gttcaaaagc ttgcatctac
    15001 ggcactaagc ccaatcagat gaaaaatatg gagtgctatt tttatctgat tgttcttact
    15061 gcttgatgca gagcagcttt ttcacaacct ctccactaaa cggtgttctc ctgccaactc
    15121 ctcggtcaca agtcacaata ttcaacaaac atgagaagct gttttttgaa attgttcctt
    15181 gtgactcgga gctaaacggc aggctcacaa cctcttggta aacggtgttc aatcagctga
    15241 ccctcggtat tagctcaaaa aacggcaaaa tatgaggaac tattttttcc gtttttttct
    15301 ctaatactca ggtcaaaacg ctgatttcac aacctctgat tcacggtgtt ccagaagtag
    15361 cttctacgga aataagccga aatttcacaa aaatttgaga agcaattttc gtaaattcct
    15421 tcttatttct tgaagctgac cacttctgtc acaacctctt attttgatta gaacttgcga
    15481 aaataaaccg gttaaagtgt gtctagcgca agagatttgc tataatagac gataagggta
    15541 aaatgtaacg agaaggggtt gaaaaatgtg tatcaaattg taacagattc ttgttgtgat
    15601 ctgccatacc atgtattagc aaaagaaaaa gttgattttt tatcaatgaa tctccaattg
    15661 aatgatcaag agttagtaga tgacctcgga aagacattca attatgaccg ctacatgaat
    15721 cagctgaaaa atggtgcgat gcctacgaca tcacaaatca atgttggccg atatttagaa
    15781 tttttccgtt cttatgcgga aaaacagatt cctgttttat atgtggcttt ttccagtgga
    15841 ttaagcggct cttacagtag tgcgcttcaa gcggttgaat tgctgaagga agaatatgaa
    15901 gaccctgaga tctatgtttt tgatacaaaa gctgcaagta ttggggaagg gcttttagta
    15961 ttggaagctg taagattgaa aaaagaaggt aaaagcatcg aagaaaccct agagtggctg
    16021 gaaagtaatt atttgaaagt ccattcatgg gtaacagtag acgatctgaa atatctagaa
    16081 cgaggcggta ggatttccaa agcaagtgct actcttggca gtctattgag tgtaaagcct
    16141 attattacgg tggataaaga aggaaggctg acgaatgttg acaaagttcg aggaagaaac
    16201 aagtcgatcc agacaatcgt caatgagacg gtaaaaacaa tcgttgcacc acttgatcaa
    16261 atcgtttatg tggcttacgc tggggatcta gagagcgcgg agaaggcaaa acggcttttg
    16321 gaagagcaga taaagatgaa agatgtgaag ctttatccct taggaccaac gatcgccagt
    16381 cacacaggct atggctgtat cgcgattttt tcgatgggaa tcagtcgata aaagcagtaa
    16441 ctcattctgt aaaaaagccc agaaattgat tgtaacaaaa acagcgactc ttaaaagata
    16501 gatgagcatc ctatctctta gaggtcgctg tttttttatt ttttaagcta aataggccat
    16561 tatgcccata actcgttcaa ttgtttttga atatctgcat tttccaaaaa ttcatcatat
    16621 gtcgtatcag ctcgatcgat cacaccttta tccgaaacgg caatgatgcg gtttgccaat
    16681 gtttggataa attgatggtc atgtgacgca aataagattg agccagtaaa tgccatcaag
    16741 ccgtcattca atgctgtgat tgattctaag tctaaatggt tcgttggatc atctaataca
    16801 agtacgttag ctttcgaaag catcattttt gaaagcatgc aacggacttt ctctccacca
    16861 gataaaacat tcactggttt caatacttct tcaccagaaa acaacattct tcctaagaag
    16921 cttcttaaga aggtgttatc atcttcttct ttacttgcat attgacgcaa ccagtctaaa
    16981 atcgttagat cagtatcgaa ttctttcgtt gtatcctttg gtaaataaga attacttgtt
    17041 gtcacacccc aacgtactgt tccagtatct ggcgtaattt cacccatgat aactttgaat
    17101 agcgttgttg ttgttatatc attttctgcg acgaaagcga ccttgtcttc tttgctcaaa
    17161 ttaaaagata tatcatctaa aattttcttt ccatcaatcg ttacacttac attttctacc
    17221 tgcaataagt cgtttccaat ctcacgttca ggtttgaatc caacgaatgg ataacgtcta
    17281 gaagaaggtt ggatatcgtc taaagtaatt ttttcaagca tttttttacg tgaagttgct
    17341 tgttttgatt ttgatgcatt cgcactaaag cgggcaataa attcttggag ttctttgatt
    17401 tgttcttctt ttttggcatt tgcgtttgct tgaagtttag ccgctagttg actagattcc
    17461 aaccaaaaat cataatttcc tacataaagt ttgattttac caaaatctag atccgccata
    17521 tgtgtacata ctttgttcaa aaagtgacgg tcatgggata caacgatcac agtattttca
    17581 aaattgatca aaaattcttc cagccaattg atcgattgga tatccagacc gtttgttggt
    17641 tcgtctagca aaagaacatc aggtttacca aatagtgttt gtgctaataa cacttttacc
    17701 ttttgtccac cagttaattc actcatttgc tgatggtgca attcttcagg gatgttcaga
    17761 ccttgcagta aaacagctgc ttctggttca gcttcccatc cattaagctc agcaaattct
    17821 ccttctaatt cagctgctcg aataccatct tcgtcagtga aatcctcttt catgtaaatc
    17881 gcatcttttt ctttcagcac ttcatacaga cgcttgtgtc ccatgatgac tgtttcaagt
    17941 acagtatagt cttcatagtc gtaatggttc tgtttcaata cggccatccg ttcatctggt
    18001 cccatagtaa cggaacctgt ggaaggctgc agatcccctg ataacacttt aaggaacgtt
    18061 gatttacctg caccatttgc accaatcaag ccatagcagt ttccgggggt aaatttaata
    18121 ttcacatcat caaatagttt gcggtctgaa aagtgcaaac tcacatcatt tacagtaatc
    18181 aatttttttc ctcactttta tcattattag ttttgacaag tcttatagaa ttatataggg
    18241 gaaaggtaaa agattcaagg caatcgaaca ttctttgtgt ttcacgtgaa acagaatgaa
    18301 aaaaataaaa ataaaagcgg agcgaaaata gctactatag gatttattag tttgcaacag
    18361 cataattttt gacgttattt ataagaggga gtactataaa actgtaaaag aaatgaaaga
    18421 aggattgaaa atggatgaaa aaagaaccat cgactggggc tcattgattt tgggaatttt
    18481 atttgtactt gtctcattgt tatcatttca agaccctgtc ggaaatttag ttgcaattgt
    18541 agtagtcttt gcgatttttg cctttattaa agggatattt gaactgtttg tacggaacag
    18601 attaaaagag ctaacaggat ataaaggaaa gatgccctta ataatcggga tcatcgatat
    18661 tctagttggc gtctttttct tattcaatat tggtgcagga gtcgcagcat tgccttttgt
    18721 ttttgcagta tggtttattg ctgattcagt tttagcgtta tttactgctg acttggcaag
    18781 aggagtaagt gaaggatatt attggtttac gatcatcgtc aatattttag gaattttatt
    18841 aggaattttc ttattgttta atccaatttc ttctgcgttg acattaagct ttttagttgg
    18901 attctatttt atgttatttg gtatcacaca tatcgtctat gcatttagat aagtaaaatc
    18961 aaacaaataa aaaagccggc ttccgattac agttttactg ttgtaattgg aagccggctt
    19021 ttttatcatg gacaaatatg cacgaggtga tttttaggtg tcgagacaaa tttatgcaaa
    19081 aatgtgagaa gctatataca caaattttat cttaatactc gaagcatagc gcagttgtca
    19141 caacctcttt tcatataatt cattcaatac tggcaaatca tggacttcat atttttcttt
    19201 gaatgcttgg atttcttctt cagaatattt tcttggatct aaatgcagat cttctactgg
    19261 caatgggcgc ttgttattga tcttcacatc gattactaca ggacgatctg attttgcagc
    19321 tgcatcaaag gcaaatcgaa gttgcgagta ttctgtgact gtaaagctat ttgccccgag
    19381 cgcttctcca actttaccga aatctgcatc ttctaaaaat acaccaaatt tcttttgttc
    19441 cgtatcttct tgttctgctt cgatgaaacc aaaagaatca ttcgaaaaga caacattaat
    19501 aattggaagc tgatatttga cttgtgtaat gatatcttgc atgaccatag caaacccgcc
    19561 atcaccactt aaggtaaata cttgtttttc tgggtagctt agttgtgcgg caattcctcc
    19621 tggcacacca ttacccatag ttgcgaacca tccagatgtc gtatgttttt gtttaccgtt
    19681 catattcaat aaacgaatag aatggatagt cgtgttaccg acatctgtga cgaaaatcgc
    19741 atcgtcttca gcaatacgat tgatttcttt atagacagcc tctggacgaa tcggttcttc
    19801 ctctcgatct tcaaaagatt tcaaccaatt tacccatttt tctttatttt cttgatttgc
    19861 taggtaccag ctatcagcag ggcgagcatc ccctaactcg acaagtcttt gcaaagctgt
    19921 gtttgcatca ccgaggatcg acaaagatgt ttcatggcgt cgtccaaatt ttgacgcatc
    19981 gatatcgatt tggataaatt cagcagttgg attaaaaaat gcacgtccaa atgggaagtc
    20041 gctaccgaca aataagatca gatcggcttc tgccaatgct tcatttccgg gtttagtagc
    20101 gacacgtcca gcaaaaccaa gaaagttttc ataatcatca ggaataattc cttttgctag
    20161 aacggaagcg acaacaggaa ctgaaaaatg ttctgagaat tctttgattt gcggaaatcc
    20221 gtttcgcgtt ccttgaccaa cataaagaac tggttttttt gctttttcaa gataaggcaa
    20281 agcagctagc aaatcttttt cttctggtaa tatcacactt gttttgtgag tgtgagcggt
    20341 ggagaaaggt tgttcgtcaa tttcttcaaa accaagatca acagggatag tcacgatagc
    20401 cacgccttta tgctcatagg cggctttaat ggcttcatct actacatgcg gaagactttg
    20461 tggtgtcatg actgtacgat tgtatacgct aacatctgcg aaaataggat tttcatttag
    20521 ttcctggaaa gagttatagt tcatggatgt agaagcaact tgtcctaaga acgctactag
    20581 aggcacatgg tccatctgtg catcatacaa accattgatc aaatgtgtcg caccaggtcc
    20641 tgcagagccg aagacagcgc cgactttccc cgttaatttt gcatctgcag cggctgcgag
    20701 tgcgccgact tcttcatgac gaacttgaat gtagttgatt tcggtttttt cttgatatag
    20761 cgcatccata attgaattga aagatcctcc gggaatgccg taaatatggt cgatacccca
    20821 gctttcaaga acttttacca ttgcgattcc tgcattgatt tttgacatgg tgtttcactc
    20881 ctttagcaat gatgatacat aaattttttg tatctagagt atagaacttt ttatcgaaaa
    20941 tcccaactaa ttcgtctttt cacaaaaatg tagtcatgtt tttgggaata tgctagagta
    21001 gtacatagat agattcaaga gaggggaatg gcatggttga atggctattg agacttgaac
    21061 cttggcagca agcacttgct gggacagctt ttacttattt tatgacagct cttggtgccg
    21121 ggttggtttt tttctttaaa gaaatcaaaa aagaagtgct aaacttgatg ttaggatttg
    21181 cttctggtgt catgattgct gcaagttttt ggtctttgtt agatccggct attacaaaag
    21241 cagaagaaaa tggagatatt gcttggctag ttgtaagtat tgggtttggt ttaggcggtg
    21301 tgtttttata tatggcagat aaaacactgc cccatatgca ttttggacca caacatgaag
    21361 cagaaggatt gcctacccat ttaaaacgga cgatcttgct tgttttttct attacattgc
    21421 ataacattcc tgaaggttta gccgtggggg tagcattcgg tgcagcagca actgcggata
    21481 atccgacagc cgctatatta gccgctgttt cggtggcgct aggtattggt atccaaaact
    21541 tcccagaagg agcagctgta tcaatcccgt tgcgacaaga aggtctgagt cgtaaaaagg
    21601 cctttgttta cggacaagct tctggaatcg ttgaaccaat tgctggagtg attggagcgc
    21661 tactagtaac gaaagtggag ctgctgcttc cttatgcact tgcttttgcc gcaggagcaa
    21721 tgatttatgt cgtggtggaa gaattgatcc ctgaagcaca gcagacattg tccagcaaac
    21781 atcattttgc agtttttggc gtcatgagtg gatttatcat tatgatgatt ttggatgtgg
    21841 cattaggata aataaaaaaa atatatatga tatatcaaat agggccgcga acttatatcg
    21901 cgaccctatt tttttagtaa gttatatcat aatgaagtga atggtatcat acattaataa
    21961 tagttttttc tatgtgtatt tcctttgatc cagcgaacaa gccagatgat aagtgcgatt
    22021 gggattagaa ctttgaccgc aaaccataaa agcccgctta ggacactgac aaaaagactg
    22081 atgacgaagc tagctgcaat tagcactaaa agaaccatga agatatttaa gaacatattt
    22141 ttttgacgca tacaaaaccc tcctcttgtt cattctttac tatagaaaaa gtataacagg
    22201 gattgtttaa aaacacctcc aactgaagac tgatttttac ttcatctact taaatgatca
    22261 aacaattgct ttttctctgg tttatcatct aaaatagagc ggaaaaggag gagaatatca
    22321 tgcctaaaaa tcgtcttgaa gcatttacag atgctgtaat cgcgattatc atgactattt
    22381 tagttttaga acttcatgaa cctaacggag acacgtggga ggcgctcgct gcattaggtt
    22441 accgattttt tatctatatt gtcagtttta tcattttagc gatttattgg aacaatcatc
    22501 atcatttatt tcaagttgta cataagataa atggacgggt cttatgggcg aacagtttct
    22561 ttgtttttgc gctttcgctt tttccgtttg caacttcatg gatcagtgag tacatcaata
    22621 gcctggctcc agaaatgact tatggttttg ttaccttgac cgctaatatt gcgtattata
    22681 tcttggcaaa agaacttgtc aaagcaaata aaggccacga gaaagcattg aaggcgatgt
    22741 tgaataatta caagaaatcg tatctatcga ttggtttgaa tgtgttagga attgtcttag
    22801 ggtatttagt agcgccagta gccgtgctga tcgcaaacat cgtgattcta gctgcttgga
    22861 tcatcccaag tcgtaccatc gaatcacaat atcggtaaaa tgatccgtgt ttaaaattta
    22921 agagaaaaat atagaaaaaa taaataaacg atcaaatatc cgagtgatac tagtattcaa
    22981 agagggatgc agatggatca aaaaagaatt catgtaggac tgattactag ttttgtcacg
    23041 atgcttattt atttggatca aggtcatttg acttggacag tagttggtgt cgtaggattt
    23101 ttgacattat tcacagcaat aaaaaaataa acatctttat gtaatctgat gttctatcta
    23161 tttaagaaaa gcatgtttac agcagtatga ttctgttgta aacgtgtttt tatttgtgga
    23221 aaatttaatt tttagaaaag tgtatcgaaa aatagatagt tagtttttcc acagtggata
    23281 acttcgtaag agataaaaat aaaaaattag tcaaagaagg gcgtatagat ttcttttgat
    23341 aggagatata caacagaaat gaataaatat taacgtgttt tcaaacagta taaaacggtg
    23401 tggtttttgg aataaaaaaa taacatatct cattttacac ttttgttatt ttatacttaa
    23461 aaaataacga cacagaaaat accgtgtctc agatttctct actacttcta aaaactactg
    23521 tttttttcct ttgaaatgcc ttcaaaaagt aggatcaggc agaatata
//
LOCUS       DANKKG010000034        23406 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000034 DANKKG010000000
VERSION     DANKKG010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.034"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(<1..161)
                     /locus_tag="PW004_001105"
     CDS             complement(<1..161)
                     /locus_tag="PW004_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HDL2726093.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYF"
     gene            complement(206..538)
                     /locus_tag="PW004_001106"
     CDS             complement(206..538)
                     /locus_tag="PW004_001106"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726094.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(552..809)
                     /locus_tag="PW004_001107"
     CDS             complement(552..809)
                     /locus_tag="PW004_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726095.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            complement(1004..1546)
                     /locus_tag="PW004_001108"
     CDS             complement(1004..1546)
                     /locus_tag="PW004_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726096.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            complement(1567..2658)
                     /locus_tag="PW004_001109"
     CDS             complement(1567..2658)
                     /locus_tag="PW004_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDL2726097.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(2666..4876)
                     /locus_tag="PW004_001110"
     CDS             complement(2666..4876)
                     /locus_tag="PW004_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726098.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(4892..7420)
                     /locus_tag="PW004_001111"
     CDS             complement(4892..7420)
                     /locus_tag="PW004_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL2726099.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            complement(7472..7882)
                     /locus_tag="PW004_001112"
     CDS             complement(7472..7882)
                     /locus_tag="PW004_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726100.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            complement(7895..8113)
                     /locus_tag="PW004_001113"
     CDS             complement(7895..8113)
                     /locus_tag="PW004_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726101.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            complement(8110..9102)
                     /locus_tag="PW004_001114"
     CDS             complement(8110..9102)
                     /locus_tag="PW004_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL2726102.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            complement(9181..10092)
                     /locus_tag="PW004_001115"
     CDS             complement(9181..10092)
                     /locus_tag="PW004_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL2726103.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            complement(10111..10455)
                     /locus_tag="PW004_001116"
     CDS             complement(10111..10455)
                     /locus_tag="PW004_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726104.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            complement(10475..11031)
                     /locus_tag="PW004_001117"
                     /pseudo
     CDS             complement(10475..11031)
                     /locus_tag="PW004_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(11112..11612)
                     /locus_tag="PW004_001118"
     CDS             complement(11112..11612)
                     /locus_tag="PW004_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726105.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            complement(11613..12158)
                     /locus_tag="PW004_001119"
     CDS             complement(11613..12158)
                     /locus_tag="PW004_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726106.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGAIV"
     gene            complement(12155..13411)
                     /locus_tag="PW004_001120"
     CDS             complement(12155..13411)
                     /locus_tag="PW004_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDL2726107.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            complement(13772..14083)
                     /locus_tag="PW004_001121"
     CDS             complement(13772..14083)
                     /locus_tag="PW004_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726108.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            complement(14106..15416)
                     /locus_tag="PW004_001122"
     CDS             complement(14106..15416)
                     /locus_tag="PW004_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HDL2726109.1"
                     /translation="MYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLV
                     RMLIDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGH
                     LLSEVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPN
                     MLIAGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEE
                     MIICLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDME
                     ARNRVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYL
                     MTFGEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYD
                     FTVKEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            complement(16030..16482)
                     /locus_tag="PW004_001123"
     CDS             complement(16030..16482)
                     /locus_tag="PW004_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726110.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            complement(16484..16849)
                     /locus_tag="PW004_001124"
     CDS             complement(16484..16849)
                     /locus_tag="PW004_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726111.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            complement(16948..21231)
                     /locus_tag="PW004_001125"
     CDS             complement(16948..21231)
                     /locus_tag="PW004_001125"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019941.1,HMM:NF037291.1,HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HDL2726112.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            complement(21378..21617)
                     /locus_tag="PW004_001126"
     CDS             complement(21378..21617)
                     /locus_tag="PW004_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726113.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            complement(21707..21979)
                     /locus_tag="PW004_001127"
     CDS             complement(21707..21979)
                     /locus_tag="PW004_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726114.1"
                     /translation="MSDGFEQVSIIKNQVREILRKKGYLVDSYFEGDYETWVGVYARP
                     ENKPTYLDPTTSEDAYLQNRYRVDGFKQDFAEWFEWEIENGEVKEE"
     gene            complement(22062..22313)
                     /locus_tag="PW004_001128"
     CDS             complement(22062..22313)
                     /locus_tag="PW004_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726115.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
BASE COUNT         6893 a         5053 c         3435 g         8025 t
ORIGIN      
        1 tcaaaataaa aagttaattc atctactaat ttattcttta tttctaaatc aactgtattc
       61 actttttctg tggcatacac acttgttcca gtactaacaa aagtacttaa acctaataag
      121 aaaacaccta aaacaccaaa tattttaatt attcttttca taatttctcc tccataaaaa
      181 ataagttact ttttaaatat cttgtttatg aattcattcc ccaaaggcct tttaagattg
      241 atacagcagt ccgttcactt cttttagact tgtcatacaa gtcttttaaa tgatcccata
      301 tcagactagt ttgtctatct agcttcaatc cattaaacgc taatgaacgg ttcatttttt
      361 ctattacttt agataaagct acagaaattt ttataccttc acaacgcgca tcttggattt
      421 caataatagt atcatcaatt agtttttgaa tttcttgcct atcaacatat tcaatcttag
      481 aaagagaatc ttttaaagct actaatgatt caacgatttc ttttttctct tcagacattt
      541 tcttcctcct tctagtgacc cgtaaagcca tgtagagcag agtccattga accagtaatt
      601 acactatcta caacgccatt ccaatttact ctacattttc catttttcag atttgtcggt
      661 ccatacggat agcctttttg acattcaatg ttcggattat accagatagg attataccat
      721 tcagccgcag aagcagattc agtcgataca aacaatccca caccaacaaa tccaatacac
      781 atcacactat agccaattag ttttttcatc tcgtatccat ctcctaaaaa atagaatagt
      841 tcttatagtt attcataaga catatttttt ccgtttttgt tttatccttt ctgtcctata
      901 acaacttttt tcaagtaaag aatgacatag tttttcttaa aaaaacagtc tgtttttttc
      961 tatttcttcg gtgtataata aatatgagtg ttcatttttt attttaatac gcactccgta
     1021 cataataaat cttgatcgca tcaatctttt gagcttcttg atctactttc aattgaagta
     1081 atacaaattg agtaacctta ttcattgttt cttctccaaa taacaaatat tgatccggat
     1141 tctctatatc ttgtgtcacc tggtaaagtt tcccagttgt tttcatttgt acatgcgggt
     1201 ctacatcaat actattgtct ttaatacaac gatctgtcat atagtccctc acagcttgat
     1261 tgcgtttata aacatctgtg aaattactcc attttctagc aaaatctttt aagaaatttt
     1321 ttgtcatttc atctatttgc atttgttgcg ctgtctcgtc ttctaatgaa ccaatttgac
     1381 tgttactttc agtactttga agagttccac ttgtttcttt tactgcttcg attgtttctt
     1441 ctgtcgttct caattgtgtt ttttgttttt cagaagaaaa ccaatgacaa ccagtaagac
     1501 ctatcaaaca taaccaacta aatactaaaa ttatcccttt tttcacgttt ctagtcactg
     1561 cctttcttat ttcaccttac gaaccagact acgaattttt gtttgattgt acgtgcgttg
     1621 ataccgagcc ataatccgtc cttgctcggc attctgttcc acactagaga aatgttgtcc
     1681 gccatatgaa acatctgtta taattccggt gtgaccatag attccaggag acaatgcacc
     1741 acctgcttgc caattcacca catctcctgc ttgtaaatca cttggttttg ggttcatgat
     1801 tacttgccaa ccataggttg tccagttata atcttcccct attaacatcg caaacatgtg
     1861 tccagaaccc attaactgtg ggccacctaa ttgagataca taccatgccg taccaccata
     1921 acattcccca ttatttacgg atcgtccaat gatccgatcc aaaaccttcg cattgccttt
     1981 actttttcct gcaataagcg aagttttttt tgttggtagg ctattccaat gcttgatcgc
     2041 aaaagtatca tattccgtta agttatagga cgttatgata ctcattgctc ctcgataata
     2101 cggactccct tcggtaatac ctgctgccgc atagcctcca cgacctagaa tcaataaaca
     2161 acttccagga tctttattgc ccacacattg attaaagttg ctttggctcg ccaccataaa
     2221 gccataatag tcataacaag cttttaagtt cggaaacttc atatagaaac caccttcaga
     2281 accaccaatt ccacgtgcag aaccttgtgg gtagggacaa ccaggaaacc aggtaatccc
     2341 aaaaaagttc gtatcgtttc ttccagattg tgactgtcca taagctgatt catagcctaa
     2401 ttgagaaaga atcacactcg gtaataactg atactttaca gctgcaccaa ctgctgcttg
     2461 cgcatgttca tctgtgattg gaccacgtcc tttaatctct gaaaaaatcg gtaatccttc
     2521 ggtatattcc ccattaaaag cgacagctag acctgtagct tctcctccat gctcagaaag
     2581 ttctcctgaa aaaaagaatg gcaatagaaa aaagggaaga ataaagaaaa gaagacaacc
     2641 tagacaccct ttcttcattt cttcctcatc ctttcttcga taatggtttt tggactgttc
     2701 gtccttttgg taaagtaaaa tcagttcctg gttttgcgtc caaaatcggc agtgttttag
     2761 gatccttttc tattttttcc gattgctctg ttggcttatc taaagaagaa aatgcaggtt
     2821 gtaacgattt tggtgcggaa atcgacggtt tactttcaga cgaacgttgc cccggttgtt
     2881 gcacagaacg atttcctgat agagtggcta ccgacgaatt aggaacagag tattggttca
     2941 ataactgctg cattgctgga taactcccat ttttcgcctg atttttcact tggcgaatct
     3001 gttgtgaaag agaaccagca gctgttaatt gtggcacggg tacttttcca gaaggcaacg
     3061 gaatcgattg accaacaaaa gcgatacctt tatttaacaa tggttttctt tgattattcc
     3121 cttgttgctt actcaattgt gccaatgatt gtacctgttg ttttggtctt actgaattca
     3181 tggacgaatt tggaatcaaa ccatttgaaa gaccagaatt ccactctgtt ttcgatttgg
     3241 tagaagacat ttcaggctct accccatgat aacgaacatg acgaccacta taaggcaagg
     3301 cttgatttga acgcccacct agttcaaagg gattaaccac ttgcctccct tttaccgatt
     3361 cttgtggtgt acgtttttgt tgaaactttt gaaatccatg tatcgctgca gaagcgagtg
     3421 gattcgttgc tcgatacaca ttccctgcta atcctgcacc tttgattgcc aaattttgtc
     3481 cagcagttga tagttgacca ggcagttgtt tcatttgaga caaagaatga cttgctatgt
     3541 ttttcgtgtt gtttatagtt tgatctgcag ctcctaaccc tagtttcttt aataaatctt
     3601 tccgttttac aatgagtaaa attgatagga gtgctttgac aaacatattt cctaaaaaac
     3661 caaaaacagt attcattgga attaaatcgt ctactatgtt atagatcaaa aatgccacca
     3721 aaataatcac actgtaaaaa gatttttgaa acatcaagcc caaaaaggac tttaaggtat
     3781 tcatgagcat ataatcaaat actggaacaa aagaagcgag taatgaaaaa ggaatcacaa
     3841 gtaataaaca caacaccaac atttgaaaga taaaacgaat catgccaatc actacaagtg
     3901 gtaatccaat acttagatta agcaatggca ccatagaacc aacgactgct ttataaacag
     3961 ctttagttgg ttgaatatag acacgataag ccttcttttt ccctctatct tctcctgcag
     4021 cagtggtcac ttttgtctca atatcagata attcttcact tgacgcttcc ttggctagaa
     4081 attcttgtgg ttcaatctct gcttgataca attcttctaa atttgttgtc ccataattta
     4141 acaatatata cggatcaaca acagccgctt tataataaag ttggcgcact ttttgaatgg
     4201 cttccttttg tgtcgttggc actaaaacac cattgacatc ttttgtcgcc aattcatcta
     4261 gactatttgt agcagaaaaa ataaagcctt ctacctccac ggacaaatca tttaaccctt
     4321 tggtaaaagc ttctccttta ctaggtacgt tactaccata gccaaaccaa accattgaaa
     4381 aaatgacaac gaagagaaat cgaataaaca ccatttttgc gtaatgtttc cctctaaata
     4441 tacgaagagt aaatagatat aacacataca agacgacaat ccccgcacca ataggatcgt
     4501 atatttttga ataaatagta tccgaaaaag tgaaaaactg gtgaattaac tcgttcattg
     4561 cagctccttc atagagcttc tcaatgacaa aatcaaggcc ttgccaaatc aatttactta
     4621 aactaaagaa catattcgtc atcattttta gaaaatctgt gccatttgcc ccaaataaca
     4681 ttttgcctaa tgaaaattca ctatcagata aataagaaga aaaggtatca atatgaaatt
     4741 tcccaatctc atagatcgat tgatccacct gcttttctgc cgcatacacc aatccaccaa
     4801 caaaaagcag tccgaatcca atcgttaaaa aaaacaaaac gcaccatttc ttttccttat
     4861 gctttatata agccattgtt tcacctcatt tctattgcat gattaacgta tcctgttctt
     4921 ctacttgaga gtgacttgtt ttttccgttg tttgtaatgc agcggtcatt tcttcaaaca
     4981 aactgtgtac attgagtttt cctacacgtc catagggatc taacatccaa cactgtcctt
     5041 taatcatgtg gctcatttca gctaagttcg cttcatttac ttccaaacgt aaatgattca
     5101 aaatcttttc tcgctcactg tcttcatcaa aagcaaagac gacaccaaaa ttcccttttt
     5161 ctttatctga acctaagtca ccaataaatt gtgtatctaa cactaattga ttcatctggg
     5221 aacgccccac acgtgccatt gctgcaataa tactacgtcc tgcgttcgaa ttattaaata
     5281 cccatgcttc tgtaaaataa atcgctgttt tcttactact atctcgtttt ccaaacattt
     5341 cacaaaaacg acctaaacta atcatcaaac acaaagactt tcgatccgca tctgtatata
     5401 gttctggtcg gacattgtca tttggtaatt tcaaatcttg gatctctaaa atcgttgttt
     5461 tggcattaaa gtctaatccc gggttttctc cataagaaaa tcctaaacgt aaaatcgaat
     5521 cttctaccgt caagcgtaaa aactcaccat attctcgaac ctctttttgt tccatttgtt
     5581 ctaaatagtc gaccacatgc atcaagccaa cttgttgtcc ggcttctttt tgaactaaaa
     5641 cttcttggat tccctttaat accgcccctt ttaaaaggtg attggtacct aacggactta
     5701 actcatcaat catatcctgg gctacttgtt tcgcatctac tcgggataaa caaacaattg
     5761 gatctaatac accataatta cggggatttg tagcatctaa cgtgacataa ctcatttttt
     5821 gcaaatgttc tacaaacaaa gggtaatgtt tctgataata cgggtcttga atgagtttcg
     5881 taaaccattt gcgcttttct cctttgggat ccacaaataa actttgtaca ttcataaacg
     5941 aactatacaa aaagagcaag ccaactaaaa aggtcttccc tttaccggtt tttccagtta
     6001 ctgctacatg aggcgcatca aacaacgcac ctttgattcc ttccgcaatg gctagaagat
     6061 tcaaaaagac aaaacgattt gacgcataaa ttaattcttc taatggtgtt ttttctcctc
     6121 gagaaccacc atcccctaat gtatcttgcc gaccaatata ccagccggat tccataccta
     6181 attctgatac tgctgaaaac aaccattcgc caactgcttc tacagttgtt cggtgcttcc
     6241 aatgtaactg ccgtcccaat ggttgcccta ataaacattt atagaacaat tctcgttgat
     6301 cattttttgc acggtggata tggataatcg tatgaagttg ttgaataacc gttgcactat
     6361 tttgacgaac ttcttcaacc gtttgaccgt atacaataaa acatgcgatc caatcgacga
     6421 caggttgccc attatctact cgttgcttta aatgttttac taatcctcga acagttaata
     6481 agcgttcact aggttcttca tcattttgaa ccaattcctt atgctctgtt ttgaaccgtt
     6541 ccgatagcca attcagtttt aaaccaatac ctccctgtcc tttcaagcgt ggatagtgaa
     6601 ttttcattcg caattctgct gggaaagaaa gatcctgagc aaattgaaat agatcacaat
     6661 ttctcatatt ttcaggtgtt tccccaatag gcaacaatgc taaataacta ctccctaact
     6721 catcttctaa ttgaagaagg cctttatgtt ccttcggtat acgtacatac gttccttgat
     6781 tgtctttcag tacttcccaa ttattttgtt cttctacttt gtgagccatt cctcgaatat
     6841 attgcagacg attgagatac aacagctctt tttttactaa gggacgaata gaaaagctat
     6901 aaagtaattg tgtcacttct tttacataag gatcactttc agaaaacaaa gtttccaaat
     6961 catctacttc taatccaaat aagcgtgcta aacttcctgc taaatctaac cagattagat
     7021 ttattacatc atgaaaactc tccaccgttg ctttttcttt gactttgatt cctaaaataa
     7081 atgaaggacg atagacttga ccttctcgtt ccaacaatgt cactgtttct tttgcataat
     7141 acgcaccaat tgcttgacta tcgggagaaa atccctttcc taatgtgtca aacttcgtcc
     7201 gtaaattcat tgttcgtgga tacatcacca actccacatc ttcataatct gctaaaagca
     7261 acagtaaacg agccatcatt tcttttattt cctgtgtctt ttttggtgtg ttttttgaaa
     7321 ttgttcgtgg aaaaacatga tagtaggccc agatacaatc ctctccattt aacaaacaat
     7381 ttttcttgaa tgcttttact ggttctttta caagcaccaa tgaatttcac ctcccaataa
     7441 aaaaggaaca accagttgat tgtccctttt tctaatcctc taaataaaca tacttattca
     7501 caaaaagtaa ttcttccaat gcattgtttg ataccttgtc ccagttataa tacacacaat
     7561 caccattcat catacgaatc atataacaaa accaatccca taaataaaaa tgcacttttt
     7621 ttccatcagg ttttagtttt gccagtgcta acacacataa ataggggagt cctatataaa
     7681 aaacaactcc tgctggtgga aaaaaagaaa tgacccaacc aataaatgaa cgtaaaaata
     7741 aatattcaaa gattacgacc aacaaaaaag ttgctgcatt gactaatgca actggaaatg
     7801 gaagacgcca attgcttgta atctgttgta cgcgataagg ttccgcataa acaatcgaat
     7861 aatcataagg ttcccaatcc acttcactat cctcctaatc cataaataat ttttcaatga
     7921 ttttaccaaa cgcatcaaat acacgtgttg gatcatttaa gaagaaataa actgcggcac
     7981 ctgctgcgat agatattaat gcatttccta cttttgattt cataaaattt cgcaatccca
     8041 gataaacaac caccattatt aaaaaatagc caccttgcgt taatataaaa tctacaattc
     8101 ccttcagtgt catttccgtt cttctccttt cctatccaac caattatgtg ttaatttttc
     8161 aacataatac tgatttgttt ttttcgtaat gacaatcgtc atttgttctt tttgtaaaag
     8221 tgacgtatca ccatctcgaa agacgaccgt tgcttttaca ataaaaggtt ttttcgtttc
     8281 tttataacgc aatactttca tctctacttg atcaacttta gctgtttgat tgagtccttc
     8341 aggttctttc atcatgtatg acatatcttt tgcattgttt ttcgcatact tatctaagaa
     8401 atcttccaca aatccttcaa catctttcgc ttccgacgca tcaaccgttt cgtataatgt
     8461 gtcatcataa gtaatcattc gttgtttttc tgtattcttt ggtagtgccg taaaataagg
     8521 ataagcaata atacgacagc ccttgtttga aatagaaaag ggaagattga gtacttgtgt
     8581 gattgtcaca cttgtttctt tcttactctc tggattcatc gagcgaatat cataagttac
     8641 tttataacta gcagttggaa tggtttcaat ttcttttaat ccaataaacg aagcagcagt
     8701 taattttcga acacctgaaa cacttcctgg ttcttgaata aagttttgtg cataaaactc
     8761 tttttttaat gtttcttgac gttgttgttc ttgttcatga ttatcgaatg tattcatata
     8821 caaagcaaca aaacgattca taaaggattc aatttcaggt gtgtacgtta attctgaccg
     8881 actttgtact tctttctttt gtaattgttg gatttcatct ttcaaaaacg acacttgaac
     8941 cttcgttttc atggattgaa aaaatgccaa gccccctgaa aaacaaagaa ataaaaccaa
     9001 acaccaaatc catttcactg ctttttttga atggattttc ttttgcttcg gtactttctc
     9061 tgtataaact ttttctttcc gccgtagttg tcgttttttc ataagctact ccttctagta
     9121 tgtttttact agtacgtacg attgacaacg ataagcttgt ttttctgaaa aatagtccat
     9181 tcaattaccc tcattttcta ttccttttct ctttctttta tatccaacaa atccagcaat
     9241 acttgtcaac actcctccta gaacagaaag tgcaacatgt atttcttcac cagtctttgg
     9301 taagactttt gtttttccat ctgctgtttt gatttcaata ttgccacttg gaagtttctt
     9361 tacaggaatc gacaaatcct ttaccaaatt cccttcacta tcttggatta atggaatacc
     9421 atcttttaca ccaacaattg tttgatcacc tgctactttt aatggggtgg ttggtgttgg
     9481 aatatgggtc aattcctcta cattactagt gacaatcggt acactcattc cttgagaagt
     9541 attcgtcgta atttcaccgc ttggggttac cgatactgtg acttcttttg ctggaacaat
     9601 aggtttattt tcttttggaa ttgcttctga tttttcaaca ggttgtttcg gttcttcttt
     9661 ttgttcttca ctggtagacg acatcgttgg ctgttccgta cttgacgaat cagtattctt
     9721 ttcagaagaa ctggttgttc ctgattgttc cggagttgtg actatacttg aatccgttgt
     9781 tgttgaacta tgcacacctt cttcttcgat cgaatcagtc ggtaaagttg attcaggaaa
     9841 ctctggttct ggtgtcactg gctctttttc aggtttctct gactgcaatt gttcaggttc
     9901 tgctggtttt tccgttgttc ctccatttag ctctgtctca acatctggta atccttcccc
     9961 tggcaatgtt tctacaattt ctgacttagg tgtttctaca gtttcatctg cataaaccaa
    10021 tgaactccct aattgaaacg aaaataatga aattcctagt gttgttacga ctgctaatgt
    10081 tgtttttttc atgattattc ctccaatttt ttatgcaatt cgtgttttgt tcatagacgc
    10141 cgcactaata ttatctgaac gataaaagaa ctcccgtccg ttttcttcta acccaacata
    10201 tggattattt ttgaataatt gaatcaatag ttcactggtg ttgtattttg gatagtttgc
    10261 aaaaaaataa tactgtgctt gtcgcccatc cgaaaattca acaagtaatt cataacgata
    10321 ccgcttcaat ccttttcgtt gttttaaagg atctctatac caacttgtcc atcgttccat
    10381 tcgtggatag agatagacac gatacacata gaacaatgcc ataccacata ccaccaaaaa
    10441 taataatcgc cacataaaaa ttcccccttt tcttctacct ttcttgaatc atcgaacatt
    10501 cataccctct atagagattt gaaaaatcca tcatcaattg ttcatataaa cgttgttgca
    10561 aatagcgatt gtttcgaata cataattctt catagccatc aatcacttga tcttcttttt
    10621 catagcatct tgtagctcta tgacgctcag cttgcatgac ataaccaaac tctgcaccat
    10681 atcgttctac tgttaataac tgttgactac gcaataatgc aatataacaa tcacaaaaat
    10741 gttcaaccga catttgattt ttctcacgtt tgtgaaatag gtattcttta gaaagcacat
    10801 tattcgttaa acaatctgat aaatagcgat aatctggcgg tcggttattt tcatctgtga
    10861 atttttccca taataaactg atttttattc gttcagcggc tagatagtct tgttcgcgtg
    10921 gataaaattc atcaatcgca caaagttttt tatcacctga ataaacaaat ccatataatg
    10981 cgaaacgaac tcttcctttt caattggctc tcctaaaaat tccagtgaca taccatcttc
    11041 tccttcctaa aaaaaataaa aaagcagaaa ccgatttggc ttctactttt ctttcctttt
    11101 atagctcttt tttacggaag aatccgcaaa tgacattttt tcaaaaaatc aatcggcaca
    11161 agttcacgaa aacttggagc aattttggat agaatcactt ccccatcttg taaaatacaa
    11221 tcataaaaat catcagttac ttgatactta cgcttcttac aataattgat aaaatagtag
    11281 cgatcatttt ctatttccat tcgttgttct actaagcgca tacgaacatc atcatagaaa
    11341 taagccacaa atcgcccttc aagttcatgt ttttcaatgg gatactcttc tggtaacaat
    11401 ctattttttc gtcgtttata cattgctgga taccttagaa gaagttcatc taaacgtctg
    11461 tcagtatatg gtaactgacg aatcaacgat tctaatcgtt ctttatacag atagaataat
    11521 tggcgcaagc aatagtgtac cgcctctgga ttttctaata ctgttggatc acgtgaccaa
    11581 tacgcaccaa cataaatcat aatttttttc atttagacaa tcgcaccagc aatactaatg
    11641 accgatccac aataggtaga acaagtggca tagcgaccaa tttcctctaa ttctatctca
    11701 atctcttcta aagaaagata tgtctgttct tctgttggct ggcgactcat ttcccaagtg
    11761 acagtcacca atgaaatttc ttccataaga ggtttttcgc tgctaagtgc tgccacacag
    11821 ttttcatata tcttcatttt atcgcagcct tttttaaccg ctaaaatcgc aggtattggc
    11881 aatgaaaatc gataaaagcg aatgaattct agccttcctt tccttcggct aaattccaac
    11941 tcttcgagtt cttgttcgta tcccttacga acttctaata agcgtgcaat ttcttgccag
    12001 ttctcttttt cctgataacg tccagctttt accgcttgtt caatataagg aactcgaaaa
    12061 cgaaaataag acaaatcaac catcttttca tttaatgctt cgattgcagc ctttaaatcc
    12121 aacaggcgca gccattctgt tgtcgtatat gcattcatgc aattttccct cctgacgttc
    12181 ccatccattc tagatagttt tgttgctcgt catttagttc ggcattatca atcccttctt
    12241 gtacaaagtc tcttcctaaa atacgatctg cttcttgtaa caactttaat gtcggagcta
    12301 cttgtttcaa aaaccagcgt tctgaacgat ctaatgagaa tttttctggc tttactgtaa
    12361 aatccaattc ttccatcgaa cgaaataatt ttgcccatcg tttatcaaca atccgtgcat
    12421 cactttccag tctatcccaa acaataagtt ttgcattaat taaaccaacg ccaatctctc
    12481 ctaaacgttt cggtgaatca cctaaaataa atgcttctac tgcttgtgtt gctttctcat
    12541 tggctaaccg taactcatat cgattataaa taccatattt cgttaatgct tctaattctg
    12601 aaattttttc tcggttcgca atttcaaaac gtttctcata aaaattaaaa aagagaggac
    12661 tctttcgcga tccaaaatac aaagataatc cttcacttcg aaaatagcca tcatctaaaa
    12721 aacaaccgcc aatattctgg aatctcttaa agtccatttc taataaacct gctttttgtt
    12781 tttctagtaa ttcaaagaga tcgaaatgtt cttccgtttc caatccccaa agttcatcta
    12841 acgcaatatc gaatcggcta cattgcacac gtgtataacc agcttccaac tttccttgat
    12901 ttttccaaat ggaatatagc catgttcgcc aatctgtttg ttgttcacgc atgacttcct
    12961 catattcccg gcaaccttgt cctgaaaatt gcacaagtac attttctgtt acagttcgtt
    13021 ttgaatgcca gaacacccag atattcccac gaacagaaag acagtcaaaa agatacaagc
    13081 ccgttggacg tgtttcaaat tctgtcagat ccattcgtaa tacacgttga atcactgttt
    13141 tgtagtctgt cccaaaaaag gaaagacgca aataatcaat cattgctcga agatctattc
    13201 cgcttttcat atacaatcga aagcgttcct tgattcgttg ttcaaaatcc tctgggattt
    13261 ttcttttccc ttgttccatc tttctaatgt aatcttttga gcagcccatc aattgcgcaa
    13321 atcttgcttg tgatagcggc tttttatctc cacttatatt atgaattgca cttaacttct
    13381 ttctgtagct ttttagctca atcggtgtca ttttctcgct ccttttataa aataacaaaa
    13441 tcgatttttt ttaacccttt agaaacaccg atatatcaac tagtccccct cgttttttgg
    13501 gaaaagtagt acaactgctg accttagata atcacttgat acaaaagggt ttataaaact
    13561 tttttctatg tgttatgtta tttctgcccc ccatattata tatcgggggg ctttacacaa
    13621 agaggccgtg cgcaagcgca cgacctcttt gtgagctgca cacgctgcta gcgagtgcgc
    13681 attataggcc catgcttcgc agccttaatg cgcaccgcca ggcagagtgc ggctaccaga
    13741 ctacgtctag agccctcccg atagataata actacaaaat taaatgatct aattttgtta
    13801 tttcactata tttttcagga gaaaaaacgc gataaattga caaagttaaa tcctcttcta
    13861 aatctactcg ataatgatac aatcgagtca atcgttctat atcttttttc gttagcaata
    13921 cttgattaat aacttttcct gtaaaaacac gttctttgcg caaaaagcgg taatcagtta
    13981 cttgttcaat acgattcaac caatcataaa acgttctgga agaaagagaa ggattaatca
    14041 ttcgtattgc tgtagctact ctataataac aactctctgc catttaactt cacctcctta
    14101 gttgattaca aaatatgcgc atcagaaaaa cttgttcctt ttggatcttt tgctaaatac
    14161 atttggcgct ctagttgttc ttgtaacaac tgattatcat ctcccatcgt ttgatcttga
    14221 ctatcctctt ttactgtaaa atcatagaca atcatttcag taaatttctc aaattctttg
    14281 ataaagtgat aatctttgta aacaaatggc gcaaaaaact ctacaggtac atcaaatcct
    14341 ggaaaagcat atccacgacc aggttgatct ttgttgtaaa atggcttatt tttaaaatct
    14401 tctccaaaag tcattaaata accaatggga gacaatcgac caacagaaat acggaatgtt
    14461 aaattatccc gaacaccact tggaaaatat tctgcatctg gtcgttgcat tgctaaaata
    14521 atgtacatcc ctgcttgacg accctttaat actaatggct gaatcaattt atcgactcta
    14581 tttctcgctt ccatatccaa cgaaccataa aaagctgccc attcatcaaa aaagataaaa
    14641 tgcggtggta acccataata agcataattt ttccctggtt catatttagg atctttcatg
    14701 gttttcatat aggcataccg ttgattcatt tcttttaatg catttcgtaa acaaataatc
    14761 atttcttcat ttattttgta aaacacatgt cctttgaata catcgacgtc acctaatgct
    14821 gctaaatctg ccgcttttcc atcgcaaata tctactgtcc caacacttaa acaagcttga
    14881 attaaagaat acaataaata ggttttccca ccaccgattg caccagcaat caacatattt
    14941 ggtgattgat catataccca atccaaagtt ttcattaaat gaatacgtcc attttctgct
    15001 atacattgat caataggaat ccgaccagaa tctgaaagga atacataatt gattcgggct
    15061 ttttctcgtt cttcacgaat taaatcacgc aaaaatactt cagataacaa atgacccatt
    15121 tttaaaaatc gttcgtgaaa acgcccacca tctaaaggaa aagaaaccac taattcttgt
    15181 ttcttttgat aaaaataaac ttttggtaat ctcatttttt cccttgtttg tttctcacta
    15241 acttgtttct ttgtataaac aaaccgattg tcaattaaca ttcgaacaag taactgacgc
    15301 caataaacag aggcaaaata tccttctttt agtcgcgacc aatgcatcaa ccctaaaatt
    15361 aacccactaa ccagcaacaa atacaacaca atacttattc caggaataag atacatttgc
    15421 caattcgaaa acgtacttaa acgtggataa aatactttcc aactaaacca acctaacaga
    15481 atcaaaaatg gaaacaaaaa gaaaccataa tagcaaaacc ataaataacg attgaaatag
    15541 cgaattcgcc gaccacgata cttgaaaaaa tgagctttca attgtcacat tcctcctttc
    15601 tgtttttact tttcctgtgc taaaaacttt tgacaaaaag caactaatac aggaatatca
    15661 catttagtaa aaaaacataa tcgaaaaaga tttaatcgat ttttataagg gtgtgcacac
    15721 atattacata ttcgtactat acgaatccaa atcacttcct gatgatcttg tttccactgg
    15781 tacggtaatg aaaaacgaac aagatcacgt aaaaacatac actgcccaat aaccgcacaa
    15841 atatatgcat ttgtttgtac aaaaatcgtt ggattgtctc caaaatactc aatcaattgt
    15901 tggatatttt gataacaagc acaatctctc aaagtatttg attggtttta tctgaattca
    15961 aaatctcccc ttccttctat cttagataaa aagaatagga agactccttc ctattctttc
    16021 aaaaaaacct tattttttct ctttttcctt agaatcaaca cttggttgtt tttgatctaa
    16081 acctttttct tgtgattgtt tcatattagg attatttgtt ggagcttcta ctttctctaa
    16141 tcctttcgca taaatcacta aatctgaata ttcacgattg ccatcttgtg ctggaatata
    16201 taccacatga tattctggat caattaattt cacaggatca cgtcgtttaa aaccagtcaa
    16261 atctacatct gctggaaatt taactcgtcg tgcaatcttt ggttcatctg gatttccaaa
    16321 aactaattgt gctcgctgtg atcgaatttc tcccgtataa tcgcctgttt cattatcaaa
    16381 tacacgatca gactcttcta atgcgccaaa gcgtacggct cctaaacaag tttcatctaa
    16441 tgcttctgta atgtcataat tatttggaaa ttttaatccc atcttacaaa acctccccaa
    16501 tttttacacc agttaaaaag acaccaacat cttgatagcc agttgttcga cgctctgttt
    16561 gtcccatatc atttgtttca cgactattca ctcgttcttt acgaaaaaca aaataagctt
    16621 cttcaaatga aatacgcacc tctttttctg gtaatcgtaa tcttttatcc tcttcagctg
    16681 tgagataaac aaaaaaagca ggctgttctt ctcgcgaaca ctctgcttgt aattgaacat
    16741 atcgcccaga cacaccatta atttcctttg gtggattagg ttcccgtgaa cccaataaaa
    16801 atacttgacc taaaaactct ttcttcatac gcaatgcttt ttctgccata aagtttttcc
    16861 tcctcttaaa aaccaaaata aattctaaat atttctacat tacaatcaaa caagcggtta
    16921 aaaatcctaa ccgcttgttc ctcatgacta atctttggct ttacgtactt tataaagtgt
    16981 tatacctaaa gcaatgacta atactactaa tccaagaacc ataagtatac tacttgtttg
    17041 ttcacctgtt tttggcaaca ttggtacaac agtagctggt ttaacccgtt gttctggttt
    17101 cactggttgc ggtggttttg gcgcttccac aatttgaacc gtttgatctt tgtttgttag
    17161 atctacattg tgtttcgcca cttcttcaaa cgtttctttc tcatcttccg ttttcgcttg
    17221 gagccattcc gcaaaactca agccttttcc tgcaagattg cgaccgtcca ctaagaattc
    17281 aaataagaat tccatcattt ctttatcggc cgcaaagact ttttccgcat gagtgccttc
    17341 aaatggtgtg ccgtctggtg ttaagtagcc ttgaacaatt aagcgataga tttgaccaat
    17401 cgcaagaccg tctactttga cccattcaag cacagtttga tttggcaagg ccaagatctc
    17461 ttttccgcca tttttattcg tcgctttcgt cgtaatggtt ggattactga ttttgatggt
    17521 ttggtcattg tcggtaatat ctgcgtgaga agccaataat acgccttctc taaacagatc
    17581 ttcaaagacc acgacttctt tgcctcgcaa cgcacgtgcg tctagaataa agtctagatt
    17641 gactgtacca tccgttgttt ctgccacaaa cgttaaggta cttgtgactt cttttccatc
    17701 tactagcact ggtttttccg ttactttgtc cattagaaca ccgttgactg tgtatgtttt
    17761 tccaacgacc aaatcgtggt atttcacagc gtctgtaatg gtgactttcg ttaatggatt
    17821 aacctctttc aagccagtgg gtttatgtgt agccgttgta cgtacacttg ggttagttac
    17881 tcgtaccgtt tgccctttgt cgttgatgtc tttatggttt gctagttctt catcatttct
    17941 aaaaagactt tcaaacacga cgatttcacg accatttata cctgtcaaat caaaggtaaa
    18001 tggtacgatc accgtttcat tttctgcttt ggcaataaag ccaacactcg tttctaccgt
    18061 atttccatgc acaagcaagg gttcaccggt tgctttatcc attaagatcc ctttgaccaa
    18121 gtatgctttt cctggaagaa tctcggtaat ttcaacggta tcgtttaagg taaacaatcc
    18181 tgttgggtct gcgactgtca atccgtcttc aaagacagcc gtggtatgaa cggttggatt
    18241 attcactttc acactttggg cttcatcgtt gatatccgca tgtgctgcaa cttcaatgcc
    18301 atcacggaaa agtttttcaa aaacaacgat cgtttgacct ttcagaacac tagcattaac
    18361 cacaaattca aggtcttttg tgccattgac tttgtctgca ataaaggcca aggtacttgt
    18421 gatttctttg ccatcttgta aaattggcgc attcgttgcc ttgttcatca atactcctga
    18481 aatggtgtat tcttttccga caaacaagtt ggtataagaa acactgtctt taatgacgat
    18541 cgtttctaat ggatcaacca ctttttcacc atcggcttta tttgacgctt gtgtttctag
    18601 ttttggtgga atcaagtaat tacgtacaga aaccactaac gtttccccat catctggcgc
    18661 attttgtttg aatgcaaccg tgaatgcttg tttatctgta tttggttgga aactttctaa
    18721 tggggcattt tccaagaagt aatagtcatc ggcaactaga cctttcacta aaatttcccc
    18781 gttttgatca gtggtatatt caccaattgc ttgattttta ccgttataca aggtaaagtt
    18841 cacacctgcg agcggcgttt tcttcttgtt ccattcttca tacttgaata attttacact
    18901 tttccgtgct tgacggttcg caaaatggat cactttgatc ggttctgtat cattggtaat
    18961 cgagaacact aatgggtttt gattcaaaac gtaatttacg ggggctttga tttcttccaa
    19021 acgatagcga tcttttccat aagtaagtgt accgttagtt aggaaaaagc ctttttcatt
    19081 ggttttaaag atatccgttg tttctgtatc atttggaatc tccattgtga cataacgatt
    19141 ggctaaagta tcaaaaatct tgaattctgt attagcaagt ggaatcactt ttcctgtttc
    19201 ttgatccact ttcaccactt taagacgttg ttcaattact ttatcttcta aaacataatg
    19261 gaaagtttgc ccattttcag aaattgtgac ttcaagtggt tcaaatagtt tatacccttc
    19321 ttttcctttg gtttcagaaa gaatgtacgt atcaaagaca acggtatttt taccaaaagt
    19381 agcataacca tcattgtctg tcacaacggt tttcaccact ttgccagttg tttttgacgt
    19441 caaagtgatt tcaatgccag ctaattttgg tttgttatct ggattcgttg aatcgactaa
    19501 atctttatta gcaactttca ctaaatcaat ggttcctttg atcacttgat cttttgcggt
    19561 cgcattgaca gtttttccag gttcaacaac aatttcaatt gcttcatcag aaagcacata
    19621 accttttggc gctttgattt cttttaaaac atatttgcca ggatcaagat ctttaatttc
    19681 tgcctgtact ttgccgttaa tttttttcaa ggtaatttct ttaatctttt tcccatctgc
    19741 ttgatacaaa ccatagaccg cattttctaa agaagctgca ccttgtgctt tcacaccagt
    19801 ttccgcatct tctttaaaca cacgaatgtt tccttgtttt agaatttgta aattaataac
    19861 agacgcatta ggttctgttg gatgtaacac accaattgtt tgagctcctg gacgacgata
    19921 ggctaatggc gcaccttgaa ctttaatttt attcaattga atttttgctt tatccggtgc
    19981 ctcttttgtg actgtaatcc atacttcatt gccgtgaata gatacattga caccttttgg
    20041 tttttgaatg tctttcacat atttcaaaaa atcattttta ttctttactt tcaacgtttc
    20101 gccaattttt accgtatgtg tttgattatt aaaagttgct ttttcaccaa agttattaat
    20161 caaacgttga atttctgctt ttcgacgatc ataatcaaca cggatatttt tgattgggat
    20221 ttttgtgccc aaagcttccc aaatcataaa ttgtgccgca atgtattgtt catcggattt
    20281 gtctttattt gtgacatatc ctaaataaga gatcaagctt gtttttttcc gtacatttga
    20341 atttttcaaa tagtttttca aagcagtagc tgtataaact tgaccttctg cggctgagac
    20401 accaggctca atacaaaacg ccacttgtcc attaatctca aaacgaacca tataatttaa
    20461 agtgaagttt agattgttcg gactagaagt aacaccaata ttttcgcccc aatcaatttt
    20521 ccgaccacct aaaacagctg cattggttgc acggcgaaca cgaaccgaac gattggttgg
    20581 tactgttact aacagtgtag gtgtacctgt ttttggaatg ttctgctctt ttttcttttg
    20641 attttcaata ctagccgtag attctttgtt tggtttcgtt ggttgatttt gactagctgc
    20701 ggttgaagag attgtttttt caacgttttc ctcatttttt ccttctttgt cagcagtagc
    20761 cgctggttgt tccactgttt cttctattcg tttcttttct tccgcttgtt tctcagaaga
    20821 ctctggctgc acagaaggcg ttgcttcggc agctttttct ccttctagag tggacgtttc
    20881 tgaagcaggg gttgtaattg aggcagtttc ttcaactggt tgtgaagtac caggactatt
    20941 ccctgggtgt tgtgtttccc caatcgtctg atcgagagtc gttttggaat tgaccgttgg
    21001 cgcttctgtc acaggggcct gttcaacagt ggcttcacca gctgggaccc cggcttctgc
    21061 tggttggact tctgtcactt cagaagcgac ttgggcaatg gcttccatcg taggttgtat
    21121 agccgtcgtt tcttctgcat gtgcttgagc agttcccacc aagaaacaga attgagaaaa
    21181 agccaccaat gcgagtgctt tcaaccaatt cattttcgtt tttgcataca caagaattcc
    21241 tccttttcct gagaatttta tataataaaa caaaacgccg ttaccaaaag tgataacgac
    21301 gtgaactgtc tcttctattt ttatctggcg ctcttatctg aacacatttc atcaatgatt
    21361 ttaccctccc actataatta cagaacaaaa aaagaacctg cttcgtggaa atcaactgtc
    21421 catttcccaa ttggaatacc agcttttttg gcaacttcac catcaccaaa tctcatgcca
    21481 taccaattac taccttcatc cttaatttct ttccaaccat atgacatagc ttcatctaat
    21541 gaatcaaaaa tcatgacact attgccaatt gttacaggat ctggcttaag atcttctgga
    21601 agagaaatat caatcatacc ataatgatcc gattcctcag tattattgtc attactagct
    21661 actggtttgg ttgttctact ggcttatttt gatacaacaa gtactcttat tcttctttta
    21721 cttctccgtt ttcaatttcc cattcaaacc actccgcaaa gtcttgctta aaaccatcta
    21781 ctcgatacct attttgtaaa taggcatctt ctgacgtcgt aggatctaaa taggtaggtt
    21841 tattctctgg acgagcatag acacctaccc atgtttcgta atctccttca aaataactat
    21901 ctaccaaata gccttttttt ctaagaatct cccgaacttg atttttaatt attgatactt
    21961 gttcaaaacc atcactcatt ctatcacctc aaataactct agattctcat aaatattccc
    22021 gacaatttca atcctatcaa tgatctctaa caaatcgcta cttatgcttc cccattcgta
    22081 taagaatttc ccttccgcaa aataaatttt ccataaactt cttcatgatc gtcccaacca
    22141 atatcactct cgaaaaattc cacgttgttc ttatctttta gctcagtaaa tgccataagt
    22201 tcaacatgtt ttccaacctc gaaacaatca caccacccat caaccgttac acgtgtgcct
    22261 tcaataccta agaaactttg tcctcgtgta ttaaacacta aaactttccg cattttttgc
    22321 tcattaatta atcacgcgcg aaatctagga atcacaactt attcgcttct tctttataaa
    22381 taaaaaaact ccaataatcg gaaattacta tcttttcctc attccttttt taaatcttca
    22441 tacttcttct tatctttgtc ctaacctaat aacacaaatt ttgaagaaaa actcagtaaa
    22501 acctgattct ttttttatct tcacaacaac caactttttc ttcagatcct tttactagaa
    22561 cttgtataca aaaattgttg aatgctgata taatttgcgg ctacttgaaa aaacgataga
    22621 tgtaaaaaga taaatagaat cattttttat tctatatcag tctatgattc attgcttatc
    22681 aatcacccaa cccattcgat gccgtttttt ttagagaaat ttaaaaaaga ccgtacgaat
    22741 ctcgcattat ccgattactt ttccagaaaa gatccccaat tctgtataaa taaccctatc
    22801 ttcttgttgg tttcattttc tcatataaga attcagtcaa tctacccact atgaccatta
    22861 ctacgtccaa cacaactgac atattaacca cgtaagtatt ccctcacaga tctccgaatc
    22921 tccatggtat ggattcccat cctgacgctg cagaggacaa catatttcat gtggctctgt
    22981 acccttttga tttggaaaca ctacaaatca atactcatct ataacttatc aagcgaactt
    23041 tcgctaggct tctagtgatt actaaattgt tggataacat acatagccca ttttatctag
    23101 ctgttattca taacagttag ttcttgtatt acttcttccg cttgtgttgc cattgagctt
    23161 gtcaaaaagt tacttagaaa aaagctgcca gtaaagcaac tttcaacaaa ccctttttgt
    23221 ttcttatcat atcaagaaac ctccttttat aaattttttc tataataaaa aacgtcgata
    23281 acttaataag tatcgacgta aactattttt atatcccgta aatcaaaaat tctaaagaaa
    23341 gatttcctat ataagcatat tggataattc gattagtatt gggtatatct ctaccattct
    23401 catacc
//
LOCUS       DANKKG010000035        22423 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000035 DANKKG010000000
VERSION     DANKKG010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.035"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            6..305
                     /locus_tag="PW004_001129"
     CDS             6..305
                     /locus_tag="PW004_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2726116.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="PW004_001130"
     CDS             378..1739
                     /locus_tag="PW004_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDL2726117.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="PW004_001131"
     CDS             1761..2021
                     /locus_tag="PW004_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726118.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="PW004_001132"
     CDS             2035..2787
                     /locus_tag="PW004_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HDL2726119.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="PW004_001133"
     CDS             complement(2881..3486)
                     /locus_tag="PW004_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL2726120.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="PW004_001134"
     CDS             complement(3539..4513)
                     /locus_tag="PW004_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HDL2726121.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="PW004_001135"
     CDS             complement(4891..5610)
                     /locus_tag="PW004_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2726122.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="PW004_001136"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="PW004_001136"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HDL2726123.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="PW004_001137"
     CDS             7161..8558
                     /locus_tag="PW004_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL2726124.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="PW004_001138"
     CDS             8639..9079
                     /locus_tag="PW004_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL2726125.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="PW004_001139"
     CDS             9227..9553
                     /locus_tag="PW004_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDL2726126.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="PW004_001140"
     CDS             9554..10303
                     /locus_tag="PW004_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726127.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="PW004_001141"
     CDS             complement(10383..10838)
                     /locus_tag="PW004_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HDL2726128.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="PW004_001142"
     CDS             complement(11265..11540)
                     /locus_tag="PW004_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726129.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="PW004_001143"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="PW004_001143"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HDL2726130.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(12978..14429)
                     /gene="cls"
                     /locus_tag="PW004_001144"
     CDS             complement(12978..14429)
                     /gene="cls"
                     /locus_tag="PW004_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDL2726131.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            14770..17436
                     /locus_tag="PW004_001145"
     CDS             14770..17436
                     /locus_tag="PW004_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDL2726132.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            17800..18552
                     /locus_tag="PW004_001146"
     CDS             17800..18552
                     /locus_tag="PW004_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL2726133.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(18574..19728)
                     /locus_tag="PW004_001147"
     CDS             complement(18574..19728)
                     /locus_tag="PW004_001147"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HDL2726134.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            19913..22354
                     /locus_tag="PW004_001148"
     CDS             19913..22354
                     /locus_tag="PW004_001148"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HDL2726135.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT         7198 a         3933 c         4673 g         6619 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaag
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag ttgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg
    12721 tttttaactt ggttggactt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    12781 aggtcgtgag cctgccgtcc agctccgagt cacaaggaac aagttcaaaa aacagcttct
    12841 catgtttgtt gaatattgtg acttgtgacc gaggagttgg caggagaaca ccgtttgttc
    12901 ggaagaaggg actgtgacaa gatttttgtc acagtccctt taatcttagt atatttttct
    12961 ctacttgaat aaaaaaatta taggattgga gaaaataatc gagaaatttg ttgcttgaat
    13021 aatagccagt tagacatttc cttgactgtt tcagttgtca tggatgaaca atgtgtctca
    13081 tcttctttga actgagtagc aagttcttct aaaaactcag gatcgtaaat gacagcactt
    13141 gtttcaaaat tcaatcggta gctacgaata tcttgatttg ctgaacctac catgcagatt
    13201 tcatcatcca ttatcaatgt ttttgcatgg aggaagccgt tctcatagac aaggatttgt
    13261 acattttcct tgatcagctg ccgagcataa tattgtgttg ctcgataaat aaaaggatga
    13321 tccggtttgt tgggaatcat gattttcaca tctacaccag aggcagttgc gatttttaaa
    13381 gcagcaatga cactttcatc aggaacaagg taaggtgtct gtatccaaac acgtttttta
    13441 gcagaagtaa tcaatttgat aaatgaaagc ttgatttgtt ccctttcgtt attcggtccg
    13501 cttgaaacaa gctggatgga tgtatcagca agcttagatt catctcgatc tgctttttta
    13561 aagaaataat tcaaatgata gccgaccttt ttttcttcag gtaccgagac gttccagtct
    13621 gttaagaagc gaagctggag caaagaagaa gcggcaccga aaatccgtat atgcgtatcg
    13681 cgccaatagc cgaatttttt tgttgtattt acatattgat tggcaatatt gaaaccaccg
    13741 gtataactaa tctttccatc gatcacaacg atttttcggt gatcatggta attcaatcgg
    13801 aaacgaagta atgccctttg tgaggtaaca aatgtatgga catgtccacc atttttgatt
    13861 agacgattga aatcttttgc ttttgtgcca tgagagccaa atgcatcata taatattcga
    13921 acttccacgc cttctgcggc tttttcttct aaaacatgta agacttgctg gccgatatta
    13981 tctgtcacaa atgcgtaata ttcgatatgg atcgaatgtt tggctttttt tagatcttga
    14041 agcagtgaat ccaatttctc ttgtccgtct gtgagaagag tgacagaatt cattcttgtc
    14101 agcggcatac gatttaatga agagaagaag tcaacaaatt gctgtttgtc tttatcgccc
    14161 atgtcaggat cgtaatgttc aatggtatct cctctaaaag actgaaagtt ttctaattct
    14221 ttcaagtcac tttgtcggag atagaatttt ttcttatccg ttaatccacg tccgaaaaat
    14281 aaatataata caaagcccac cccaggaagg gcaaacaata ctaagagcca tgcccaaatg
    14341 gctgctacat ctcggggttt gatcaaaata gccaccaaag caataagtgc atttaataga
    14401 taaagggcgg ttataatact agataccaca aaaatgttcc tccttacata caataacatt
    14461 agtatagcat gatagaagaa agaagcctac caaggtgaat atacttttca ctcctttcat
    14521 gaaaatgtag gtaaatcggg ctaaagtagt tgctttttgt aggttaataa ggtaggatat
    14581 tagtacagtt gaatatctcc ttaggggagt aaccggcaac gtttgttgcg ataatatcaa
    14641 cagtaagtta accaaatggt tttctggtat tgtatgaaat ggtgagacct aagacaaatg
    14701 cgtatttgtc ttaggtctct tttttttcag acaggactag ggccaagatc aaaaaatggg
    14761 ggatgaaatt tggaaaacaa ggaacaagca caacaagcgg tctataaaga aacgatcgac
    14821 tcgttgatgc agcaaacgca gagctcaacc gatggattga gcaccacgca agcgaaggaa
    14881 aaattagaaa gagatggctt gaaccagcta aaagaaaagc cgaagaagcc aacttggaaa
    14941 ttgttcttag aaactttcaa agatgcaatg gttatcgtat tgctgattgt cgcactgatt
    15001 cagatggtca tgggtgacta catcgaattc ttagttattt ttgcagtatt gatgttgaat
    15061 tctataatca gtgttgtcca aacaaaaaaa gcagaaggtt ctttagaagc gttgaaaaac
    15121 ctttcagcgc ccgatgctcg agtgattcgt gatggtaagg aaaaaacgat tccggcaaac
    15181 gaacttgtgg taggagatat cgtgttgtta gacgcaggcg attatgtacc tgctgatgga
    15241 cgtttactag atgcgggatc attaaaagta gatgaaggta tgctgacagg agagtcgact
    15301 tctgcagaaa aagaagtaac ggatatcaca agtacggtac caatcggaga tcgtacaaat
    15361 atggtattca gtggaacgat cgtaacttac ggacgcggta aattcttagt gacttcaact
    15421 gggaataata cggagatcgg acaagttgct ggccttttgg aatcaacaac tgagcaagtc
    15481 actccgttac aacgaggtct ggataaattc agtaaaaaac taagtttggc tattttggca
    15541 ttgtctttat tgattttagg tatccaactg tttcgtattt atcttggaga aggtacagga
    15601 gatatgactg cagatatcgt cagcgcagtg atgtttgccg tagccgtagc cgtagctgct
    15661 attccagaag cattacagtc tatcgtaaca atcgtgttat ctttgggaac gaataaaatg
    15721 gcaaaacgtc atgccattat tcggaagctt ccagcagttg aaacacttgg ttcgacaagt
    15781 atcatctgta cagataagac tggtacattg acacaaaaca aaatgacagt tgttgactac
    15841 tttttaggta atggcgatac tggggatttc acaaatgacc cttcaaaatg gacagctgac
    15901 gaacgtcgct tgatcgaaat cagtgtttta gcgaatgatg catcgatcag tgaagacggt
    15961 actaaattgg gcgaccctac ggaagtagcg tttatcgact ttagtgaaaa attaaaccag
    16021 ccttatcagg aaatccgaaa caactaccca agacaagcag aacttccttt tgactcagat
    16081 cgaaaattaa tgtctactgg acacacaatt gataacaaaa attacttgtt tgttaaaggc
    16141 ggaccagata tcgtcttcca acgcagtaaa aaagtattgc taaatggtga agtcgttccg
    16201 atgacggatg aattgatgaa aaaattccaa gagcaaaatg aagctttttc aacacgcgca
    16261 ttacgtgtac tagcttttgc ttaccgtcca cttgataatt ttgatttatc atttgaagat
    16321 gaagacgatt tgatccttgt tggtttgctt gctatgatcg atcctccacg tgctgaagtg
    16381 actcaggcgg taacagaagc aagaagtgca ggaatcaaaa caatcatgat tactggtgac
    16441 cacaaaacaa cagctgttgc tatcgcaaaa caaatcggga tttttgaaga aggaaacctg
    16501 gctttgaccg ggacggaact agatgaattg acagaagatc aactaaacga agttttagaa
    16561 aaagtcactg tttatgcacg tgtttcacct gagaataaaa ttcgtatcgt tcgtgcttgg
    16621 caaaataaag gtcatatctc agctatgaca ggtgatggtg taaatgacgc accagcattg
    16681 aaacaagcag acatcggaat tgctatggga acgggtacag atgtggcaaa agatgcagca
    16741 gcgatggtat tgacggatga taactttgct tctattgtca gcgcagtaga agtaggacgt
    16801 aatgtgtttg ataatatcaa gaaagctatt tcttatctat ttgcaggaaa tttaggtgcg
    16861 atcattgcga tcatctttgc cttagttgct gactggtcca atccatttac agcattacaa
    16921 ctgttgttca tcaatttagt caatgattca ctgccagcaa ttgcattagg tacagaaaaa
    16981 gctgaacctg ctacgatgaa ccgcccgcca agagatccaa attcaggaat ctttacggac
    17041 aaaacattga tttctgtaac gtatcgtggt gtgctgattg ggattgctgt tatctttgct
    17101 caatggcttg gcatgcagca ttcacctgaa ttcggtgtag ccatgtcatt ttcaacattg
    17161 atttggtgtc ggacattgca aacattccca gcacgttcaa acacacaaac agctatccaa
    17221 gcagggttct tctctaacag aagtgtctta ctagctgtag ttgtatgtag cgcgctttac
    17281 tgcttaacaa tgatcccagg actgaaagaa gtcttctcta tcccatcagc atttggattg
    17341 acagaagtct ttacatgtat gggaatcgca cttgtgacaa ttgttttgat ggaaatcact
    17401 aaattgatca ttgtacgagt acaaggaaat aaataaaacg aatcaacgag gttgtgagcc
    17461 tgccgtttaa ttctgagtca caaggtgcaa tttcaacaaa cagcttctca tgtttgttga
    17521 atattgtgac ttgtgaccga agagttggca ggagaacacc gtttaaggaa gaagttgtaa
    17581 aatcagcatt ttgagctaat actgagggtc atctgattgg acactgttta ttcggaaaga
    17641 gggactttga cacgactttg gtcacagtcc ctttttttat ttatgataga cggaaagcca
    17701 atcattttat tattcatcaa aagagataga atagataaaa gaggagaatg atttattccg
    17761 aaaagtgaaa acatataaca aaaaattaga ggaaatcaaa tgaatgaaaa agaatgggat
    17821 gaatttgctg aagaatatta tgaaatccag caagaatcgc aagtaactat tgctgaagat
    17881 gtaaaaaatt atctgaaaac ggagaaagtc ctgcctgctg aaacagcagc agacgttgct
    17941 ggaggatctg gcagatttct ttccttagca aaagaaatta atcgctatga tttgatcgat
    18001 ttttcttcag gaatgctaga aaatgcaaaa aaagaagcag aaaaaaatga gttgccaaat
    18061 gtttccttta tcaaacagtc gttagaagaa tttatccaat caaatgagac atatgaaatg
    18121 gtttttacag cagcaaatcc agcattggta catcttattc aattagaaaa attgatgaaa
    18181 aagtcaagca aatggtgcgt gataatcaga gtagtggaat caatcgatga tgttttttat
    18241 cctatcgaaa agcaactagg aatgatagaa gaagatccta atacatcgcc agttgtaatg
    18301 gatcaatttg aaacatactt aattgatgaa ggattcaaag tgaagaaaaa agattttact
    18361 tattttacac aggaatcaat tactgaagaa ttgatgaagc tatactatga cgcgtacgca
    18421 aaagatcctg ttgtgaccag gtacattcgc aaactattca gtgaaaaacg agaaatcctt
    18481 agtacgacta agctgaccta tagactgctc ctttggtccg cagataaaaa aagaaccttt
    18541 cacgactcgt gaagggttct ttttatgatt ttattatatt ttgtagtaac gaatatcatt
    18601 ttcgatcttt gtaatcgaaa aaggtacatc tgaagtatat tctgcacatt ctccgtgttc
    18661 aggaagcgtc tcactgaaaa tcgtttcata ttcgtcaaca gtgatccgtg ttcgagaatc
    18721 tagcatctct tgatgagatt gtgcgaagag atattcttga tagttttctt ccagataacc
    18781 tgtaaaaaat tcacttactg caccagaacc gtagctgaat agaccgatgc gatctcctgg
    18841 ctgtagtgat ttcgaatttt ccagtagaga agttagcccc aggtataatg aaccagtgta
    18901 aagattaccg attcgtcggc tgtaacggat gctttcttca tagcgagcca taagacgttc
    18961 ctgattttct tcgtctgttt ggtctaatac gctttgcaat gccttttttc ccatcttagt
    19021 atacggaata tggaaagcaa tcgcctgata atcttcgagt cctcgacccg acaattcttt
    19081 atgtcgattc caaacttttt ggaatgattc gatatacgta gaattagata aaggaccatc
    19141 aacaacagga aattcgctat aatctggacg ccagaaatca tagatatctt ctgtcagaaa
    19201 tacgctgtcg tcttcaatcg ataaaatacg cgggttttga gtgatcatca tcgcaacagc
    19261 accgacacct tgcgtcactt caccaccgct tgccaagctg taacgagcaa tatcacttgc
    19321 tatgactagt acttttcgtt ctggatgatt tttgacatat tctttcgcca tgtgcaaagc
    19381 tgcggtgcca ccgtagcaag cctcttttac ttcaaaagaa cgagcaaaag attggatttt
    19441 cagtaaatgg tggataatta ccgcactcgc ttttgaatga tcaatcccgg attctgttcc
    19501 gacgatgacc atgtcgatta gctcacggtc tttttctgtt acgattttgc tggcagcatt
    19561 tgctcctagt gtaatgatgt cttcatttgc acgattgact gccatttggt cttgaccaat
    19621 ccctatatgg tattttgcag gatcatcccc acggctttct gccaactctg tcatatctaa
    19681 atataaatta ggaataaaaa aggaaagacg atcaatccct attttcattt caacacctct
    19741 ttacttatga taaaaaactt actttactaa ggtatcataa aaagattaaa agaaatataa
    19801 aaaagagaat atttaaggat tgttaaaatt taatcttaga aaaaaataac tggaacaaga
    19861 gctatttgtt tttacagcaa agaatggtat atttttgtag gaggtggctt acttgaaaga
    19921 agtcgttatg atagatgctg caagaacgcc gataggaaag tatcgcggaa gtttaagtcc
    19981 atttactgca gttgaattag gcacacttgt gactaaagga ttattagata aaacaaaact
    20041 gaaaaaagat aaaatcgatc aagtaatatt tggaaatgtc ctgcaagctg gtaatggtca
    20101 aaatgttgcc agacagattg ctttgaacag cggtctccct gtagatgtcc cagcaatgac
    20161 gatcaatgaa gtttgcggat ctggtatgaa agcagtgatc ttagcacgcc agctgatcca
    20221 attaggtgaa gcagaacttg tgatagccgg cggaacagag agtatgtctc aagcacctat
    20281 gctgaaacag tatcagtcag aaacaaatga atatggtgaa ccaatttcca gtatggtcaa
    20341 cgacggattg actgacgcat tttcaaatgc acatatggga ttaaccgcag agaatgttgc
    20401 aacacaattt tctgtgagca gagaagagca ggatcgctat gccttgtcgt cccagttgaa
    20461 agcagcacat gctgtcgaag ccggtgtatt ttctgaggag atcatcccag tcaagatttc
    20521 tgatgaagac gtgttatctg aggatgaagc agttcgtgga aatagtacat tggaaaaact
    20581 gggcacgtta cgtacagtat tctcagaaga aggaactgta acagcaggaa atgcttcccc
    20641 gttgaatgac ggtgcctctg tggtgatcct tgcatccaaa gaatacgcag aaaataataa
    20701 tctgccttat ttagcaacca tcaaagaagt agcggaggtc ggtattgacc caagtatcat
    20761 gggaatcgct ccaatcaaag ccattcaaaa attgacagat agatcaggaa tgaatttatc
    20821 aacaatcgat ctttttgaga tcaatgaagc atttgcagct tcttctatcg tagttagcca
    20881 agaactgcag ttagatgaag aaaaggtcaa tatatatgga ggagcaatcg cattaggcca
    20941 tccaattggt gcaagcggtg cacgtatctt gactacatta gcttacggat tgcttcgtga
    21001 acagaagaga tacggtattg cttcattatg tattggtgga ggtttaggct tggcagtctt
    21061 gttagaagcg aatatggagc aaacacataa ggatgtgcaa aaaaaaaagt tttatcagct
    21121 tacgccatct gaacggcgca gtcaattaat agaaaaaaat gtattgacac aagaaactgc
    21181 gttgattttt caggaacaaa cactttctga agagctctcc gatcacatga tcgaaaacca
    21241 agtgagtgaa gtggaaatcc caatggggat cgcgcagaat tttcaaatca atggcaagaa
    21301 aaaatggatt ccgatggcaa cagaggaacc atctgtcatt gcagctgcaa gtaatggtgc
    21361 aaaaatctgt ggaaatatct gcgcagaaac acctcagcgg ctgatgcgag ggcaaatcgt
    21421 tctatcagga aaatcggagt atcaagcagt gatcaatgca gttaatcatc gaaaagaaga
    21481 gctgattctt tgtgcaaatg aaagttatcc ttcaatcgtg aaacgcgggg gcggtgttca
    21541 agatatctct actcgggaat tcatggggag tttccatgca tacttatcta ttgatttttt
    21601 agtggatgta aaagatgcaa tgggcgctaa catgatcaac tcgattcttg agagtgtggc
    21661 aaacaagtta cgggagtggt tccctgaaga agagattcta ttcagtattt taagtaattt
    21721 tgctacagaa tcattggctt ctgcctgttg tgaaatccca ttcgaacgct tagggagaaa
    21781 caaagaaatc ggtgaacaaa ttgccaaaaa aatccaacag gcaggggaat atgcgaagct
    21841 tgacccttac cgtgcagcaa cgcacaacaa agggatcatg aatggtatag aagctgtagt
    21901 ggcagctaca ggaaatgata cacgtgcggt tagcgcttcg atccacgcct acgcggcaag
    21961 aaatggatta taccaaggat taacagactg gcagatcaaa ggggataaac tagttggaaa
    22021 actaacggtg cctttggctg ttgccacagt aggcggagct tccaatatcc tgccaaaagc
    22081 aaaagcatct ttagctatgt tagatattga ttcagccaag gaactagcac aagtaattgc
    22141 tgcagtaggg ttagctcaaa acctggctgc tttgcgtgca ttagttacag aaggaatcca
    22201 aaaaggccac atgggacttc aagctcgttc attagcgatc tcaattgggg ctattggtga
    22261 ggaaatcgag caagttgcga aaaaattacg tgaagcagaa aagatgaacc agcagacagc
    22321 tatacaaata ttggaaaaaa tccgggaaaa ataaaaaagc aatatcttta gaagaataaa
    22381 gcaagattta cagttatttt atttactgca gatcttgctt ttt
//
LOCUS       DANKKG010000036        22339 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000036 DANKKG010000000
VERSION     DANKKG010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.036"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(44..1936)
                     /locus_tag="PW004_001149"
     CDS             complement(44..1936)
                     /locus_tag="PW004_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2726136.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(1933..3660)
                     /locus_tag="PW004_001150"
     CDS             complement(1933..3660)
                     /locus_tag="PW004_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2726137.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3676..4326)
                     /locus_tag="PW004_001151"
     CDS             complement(3676..4326)
                     /locus_tag="PW004_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HDL2726138.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4530..4826
                     /locus_tag="PW004_001152"
     CDS             4530..4826
                     /locus_tag="PW004_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDL2726139.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5018..5638
                     /locus_tag="PW004_001153"
     CDS             5018..5638
                     /locus_tag="PW004_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HDL2726140.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5730..6896)
                     /locus_tag="PW004_001154"
     CDS             complement(5730..6896)
                     /locus_tag="PW004_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL2726141.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(6908..7261)
                     /locus_tag="PW004_001155"
     CDS             complement(6908..7261)
                     /locus_tag="PW004_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDL2726142.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7389..8411
                     /locus_tag="PW004_001156"
     CDS             7389..8411
                     /locus_tag="PW004_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HDL2726143.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8430..8591
                     /locus_tag="PW004_001157"
     CDS             8430..8591
                     /locus_tag="PW004_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726144.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(8850..9284)
                     /locus_tag="PW004_001158"
     CDS             complement(8850..9284)
                     /locus_tag="PW004_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726145.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9394..11253)
                     /locus_tag="PW004_001159"
     CDS             complement(9394..11253)
                     /locus_tag="PW004_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDL2726146.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(11371..12531)
                     /locus_tag="PW004_001160"
     CDS             complement(11371..12531)
                     /locus_tag="PW004_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HDL2726147.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(12838..14262)
                     /gene="araA"
                     /locus_tag="PW004_001161"
     CDS             complement(12838..14262)
                     /gene="araA"
                     /locus_tag="PW004_001161"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HDL2726148.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14278..14976)
                     /locus_tag="PW004_001162"
     CDS             complement(14278..14976)
                     /locus_tag="PW004_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDL2726149.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(14933..16594)
                     /locus_tag="PW004_001163"
     CDS             complement(14933..16594)
                     /locus_tag="PW004_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HDL2726150.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            16812..18212
                     /locus_tag="PW004_001164"
     CDS             16812..18212
                     /locus_tag="PW004_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HDL2726151.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18464..19543)
                     /locus_tag="PW004_001165"
     CDS             complement(18464..19543)
                     /locus_tag="PW004_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2726152.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19629..20588)
                     /locus_tag="PW004_001166"
     CDS             complement(19629..20588)
                     /locus_tag="PW004_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HDL2726153.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20600..22111)
                     /locus_tag="PW004_001167"
     CDS             complement(20600..22111)
                     /locus_tag="PW004_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HDL2726154.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22133..>22339)
                     /locus_tag="PW004_001168"
     CDS             complement(22133..>22339)
                     /locus_tag="PW004_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726155.1"
                     /translation="SENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIFLFP
                     PFIFIFAGGVWKLVSIILTIGNER"
BASE COUNT         6354 a         4735 c         3942 g         7308 t
ORIGIN      
        1 cttttgtcac agcccctaaa aaaaataata aatttgtact attctatgaa gcttcttctt
       61 cagaaaactg actgttataa agatctgcat agaatccgtc tttttccatc aatgtatcgt
      121 ggtttcctgt ttctacgatc gaaccatgat tcatcacaat aatgttgtct gcatcgcgta
      181 ttgtagacaa gcggtgagca acaacgaaac ttgtccggtt ttccaatagg cggttcatcg
      241 ctttttgaat cagtatttct gttcgtgtat ctacactaga tgttgcttca tcaaggatca
      301 ggacatctgg atttgctaag aatgcccgcg caattgtgat tagctgacgc tgcccttgcg
      361 aaatattgct tgcatcctcg tttaatacag tgttatatcc ttctggcaat ttacgaacaa
      421 aatcgtctac gtgagcagct ttagcagcct caatgatttg ttcgtctgtt gcatcgtcgt
      481 tcccatattt gatattgtca tagatacttc ctgtaaacag ccaagtatct tgcagtacca
      541 ttgaaaactg tgcgcgcaat tcatcacgtg aaagatcacg ggtatctaca ccatcgtaac
      601 gaatactacc tccagaaaca tcgtagaacc gttcaagaag gttgatcagt gtcgtcttcc
      661 ctgcaccagt cggacctaca atagcgacca tctcaccagg tttgacatcc aagttaaaat
      721 cagtcattag caagttatct ggtgtgtagc cgaattgaac attttcaaaa cgaactttgt
      781 atggtgtatc tttttcttct ggaaggccag atttttcatt ggacatctct tcttcatcca
      841 acacttcaaa tacccgttcg gctgaagcaa ctgttgattg gatagtgttc atcaaacttg
      901 caagttgagt gattggttga gagaactgtg ttgtatattg caagaacgct tgaacatccc
      961 ctaatgtcat gttcccattt gcaactttta ctccgcctag tacagcgacg aacacatagc
     1021 ctagattttt aacgaaattc atcaacggca tgatgattgc cgaaataaat tgagctttcc
     1081 aaccagcggc atacagattc tgattttctt tttcaaatac ttcctggtct ttttcagtat
     1141 gattgaatgt tttaacaatg gtatgaccac catatgtttc ttctacttgg ttattcaata
     1201 aacctaaact tttttgctga tctgcgaaaa acacttgtga tttcggcgct acgatcatca
     1261 cgatgatcaa gcttaaaggg atcatcaata acgcgactaa cgtcaattgc cagctgattg
     1321 aaagcatcat cacgatgacc ccaatcaatg tgactgcact agttacaaat tgagtcaaat
     1381 tttgctgcag cgtgcttgcg atgttatcca tatcattgat tgctcgagac atgatatccc
     1441 catttgtatg tgtatcataa taggaaatcg gtacacgatt cattttggct tccaaatcat
     1501 gacgtaattc ataaactgtt cgttgggaaa cacgtgtcat aataaattgc tgtaaaaagt
     1561 tgaatacagc agaaatcaaa tacatcacga ttactgtcgc aatgatctgc gcgattttat
     1621 caaagtctat cggaaatttt gttacttgtt ggccttgttg catctgttga gcgccagcca
     1681 ttacaccttt gaatatttct gtggttgctt tccccaagac ctttggtgtt tggatttgga
     1741 agatcacggc tgctattgct agtacaaata cagcgatgat cgcaaccata cgtttagaca
     1801 tatagccaaa caatcggcgt accgttcccc agaaattttt aggcttttca ccttttacac
     1861 cgagatttct gccaggtcca ccatgtcctg gaccgcctgc tggtccatga gagggacgtg
     1921 tgtttgtttt actcatgcta agtcctcctc tcttagttgt gaatgaacga tttcttgata
     1981 agtttcgttt gttgctaata gttcttcatg tgttcctttg ccaaccagtt ttccttcatc
     2041 tagcacaagg atcatatctg catccattac tgtacttact cgttgagcaa ctaaaacggt
     2101 gatactttct ttcatattcg ttttcaatgc ttggcgaaga ttggcatccg ttttgaaatc
     2161 caacgctgag aaggaatcat caaacacata gatatctgct gttttgacta atgcccgtgc
     2221 aattgccaga cgctgtcttt gtccaccaga gaagtttcct cctccttgtt ccacatggct
     2281 atctaagcca tcttccatct ctgaaacaaa atctttagct tgtgcgattt ctaatgcttt
     2341 ccaaatcatt tcatctgttg catccggctt accatactgc atattgtctc ggatcgttcc
     2401 agagaacaga actgcttttt gaggaacaaa gcccattaat tctcgcaaat tatattgagt
     2461 catgtcacta atgtccgttc catttatttg gatcgaacca gattcgatgt catataatcg
     2521 agggatcaaa ttgactaagg tcgttttacc tgaaccagtc ccccctatga ttgcgacgat
     2581 ctcgcccgac ttggcttgga aatcaatatc ttccaatgca agtttttctg ctccatgata
     2641 tcgataattg acatgattga aggccaatgt tgcgtttttt ccagcaaaag aaactgtttt
     2701 aggattttca gggtcatgga taccgatttt ttcatccaac acttcattaa tacggtcagc
     2761 tgaagcttgt gcacgaggca ccatgataaa aatagcggaa agcatcataa agctgatcaa
     2821 gatctgcatc gcataagtca taaatgcaat cagattaccg acttccaaag tcatatctgc
     2881 tatgtagtgt ccaccaaacc aagtgattgc tacgtttgta ccgctcatga tcaatgtcat
     2941 cattggcatc atcaatgcga cgatcgtatt aactttgatt gccgtatttg tataatcttt
     3001 attcgctaaa tcaaaacgat tttcttcaaa gcgtgtctta tcgaatgctc gaatcactct
     3061 gacccctgtc aatccttctc gaaataccag gtttaaacgg tcagttttct tttgcatact
     3121 tttgaataat ggaacagcaa agtacatgat tccgccgatc aaaataatca ttactgggat
     3181 gacaactaag aagatttttg tcaactgatg atctttttga taagcaagta tgctcgcgcc
     3241 aatcaacgtg attggtgcat tgatcatcat acgtaaaaac atttgtgtaa ccatttggat
     3301 ttgagtcaca tcattggtcg ttcttgtgat caaagaggcc gttccatatt tatcaaacgc
     3361 atcttttgtc agactctctg attttttata gacatccgta cgcaattgct tacccagttt
     3421 ctgtgattca cgagtagcaa aaaatgtatt tgcgatagca gctaagatac tgatcaaaga
     3481 aaagccgatc atcacaaaac ctgtatgcca aatataatcg acatctcctt gcgctacccc
     3541 tttatcaata atattggagg taagtgtcgg caaatataaa tctgccagta cttggatcac
     3601 catgaaaact gcagctgcaa ttgctgaagt caaggagatc cgttttacga tttttagcat
     3661 ctagttccct tctttctatt tatatactcc gtttttcagc caagaaagtt tcaaattgaa
     3721 ttcactgatt gcttgctcta ctgagtaatc ttcagatgtt gaaaataagc gatacgaatg
     3781 cgcaattgtt gtaaatacag tattcatcac taattggatc agtaattcca attcatgatc
     3841 attttttact ttcagcttcg tccgatcgat caattcaagc aactcttttg catcttgttt
     3901 gtgttcttta tgtggatcgt gaggcgtttt ttggcgactc gtatgaggag cgacttttct
     3961 tgaggcacgg taatccatgt tcataaacaa gttacgataa aaagaagcat tctcgccttc
     4021 caaaatctca taaatcattt tagaaaaata ggattcgaat cctttgaata gatcaccatc
     4081 ggattctttc attagttgta acatatcttt agagctgtca cgtctcattg tctcaaagta
     4141 ataaaaataa agatcctctt tatcttcaaa atactgatag aaactgcctc tagggatatc
     4201 cgctaatttg acgatattag caatcgaagc ttcttttaag ggtactctag aaaactcgat
     4261 tctcgcagcc tcgattagtc ttttttgttt ttctttaggc aaatggaaaa atgtctgtgt
     4321 cggcatatgg attctccttt ctttcaagat gacacattgt catatgacgc tatgtcatta
     4381 taacacctcc tacctgtttt gcaagacatt tgcatagttt ttttcattat catccgatac
     4441 attaaaaaag tgataagagg aatccttcca tttgaattaa tgagagttgc ccattaaaat
     4501 agccacatac taaacaaagg atgagataaa tggcccaatc aattagtgtc caagacttca
     4561 ataaattgcc tctcgatgct gacagcatcg ttttagatac aagaaatacc ttagattaca
     4621 caaaaaacca cttaagccac tcgctttcta tccccgctgc tatcttgcca agacaattaa
     4681 aaaatctaga tccaaagaaa ctttattata ttctaagcta tacaggcagg cgatcagaag
     4741 cattagccga tcaacttact gctaatggtt ttcgtgctgt ttatatcatt ggtgggatgg
     4801 aagctcttca gtcgagtgtt gcttgaatat tgcttgttag attcaaaaga ggctgtgaca
     4861 aaaaatcgtg tcacagcctc ttttataccc tgtctttttt tagttattta ttcttcaacc
     4921 ttatcaatat gacttgatag ccttttcttt ttcgagtaaa ctgattttgt tagtcatagt
     4981 tataacaatc atagcaataa aaggagaagg taagcatatg gcatctgttt tagtagtcaa
     5041 aggtcatccc ctaactgcag aagaatcgcg cacagttaag gcattgacgt cttttttaac
     5101 aagttataaa gaaaatcatc cagatgatga agtgactgtt ttggaacttt atcgcgatga
     5161 tattccggaa atcgatgaag aattattatc aggttgggaa gcattacgtg ctggtgcaga
     5221 attcacttcc ttatcagaaa gtcaacaaca aaaaattgct cgtttcaatg aattgactga
     5281 tcagttcctt gctgctgata aaatcgttat cgcaaacgcc ttatggaatc taaatatccc
     5341 aacaaaatta aaagcttggt tcgataccgt caatgtagct ggaaaaacgt tccgctatac
     5401 agaaaacggg ccagaaggat tagtgacagg taaaaaagca ctgcacatcc aatctaatgg
     5461 cggtgtatac aacggacaag actttgcctc tcaatatgta aaaggtattt taaactttgt
     5521 aggcatcgat caagtagatc agctattcat cgaaggaatc gattatgacc ctgaccgtgc
     5581 agatgagttg atgcaaaatg cattagataa agctgctgct ttaggtaaat cattttaaaa
     5641 attgagataa atgcatacaa aagagacaga ggaaaaaatc ttcaaagcag catttgtgcc
     5701 ttggatattt tcccatctgt ctctttttgt tattctttct gctttttcaa cggattttct
     5761 tcataaagag aagagattgt tgtgtaccat tcaaaaacat gagtaatcac tactttagct
     5821 gctgcataaa caggaatccc caagatcaca ccgatgattc cgaacaattt cccagaagtc
     5881 agcagaacta ataaaatcgt tactggatgg attgctaact gattccctag tactaatgga
     5941 gagatcaagc gtccttctat tgtctgctct actgcaaaaa cgatcagtac tttgatgatc
     6001 atgaatggac ctgctacaat ggctaaaaag atagccggaa tcatcgctaa aaatgatcct
     6061 aagtaaggga tcaggtttaa aaaacccgca ataatcccca acgtgacagc atactctaaa
     6121 ccaatgaccg caaaaccaat cataaacatg attgccacag caaaagccac agttagttgt
     6181 cctcttatat aagaagaaac ttgactattc atttcactca gcacattcaa cgtcggtttt
     6241 tgcatgcgtg tcggtaaaaa tttgacaaaa taaggtgcta attgtttacc gtctttcaat
     6301 agataaaata agataaacgg catcgtcagc agagccacta aaatagtcgc taccgcgccg
     6361 acaaaacttc caagactctg aactgtactc ttagatatat cttggatcat atcgcctgcc
     6421 gaattcatga acttatcacc tgctgtttcc aactgttcgc gaaattggtt gaacagcggg
     6481 tcccgcaaaa tctcggttaa tttattatca atcgtatcta catattgagg gaagttactg
     6541 atgaagctca ctgtttgttc ctgaatctta gggatgatca ctacgctccc ccatgcgatt
     6601 agtgcaacaa ccaaaacaaa cagcccaatg atactgtaaa gccttggtac ttttcgcttt
     6661 tcaaagtaat caactaccgg attcatcaag taatacaaga ttcctgctaa aatgataggc
     6721 agacctacga ttgctaaaaa ttgccagatt ggtgcaaaca aatatgaaac ttttgtaaat
     6781 aaaaagacga tcagcaatat taacaataca acaagtaagg ctgtgactac ttgattattt
     6841 aaaaaccagc gccaaaacca cgataccggt ctgttcttct tattttttcc ttccataatt
     6901 cctcctgtta gttataaaaa atttggcttc ccaaaccaat agcaggcagt ctatgcggat
     6961 gaagatagat tccatttgca atttgtcctt cttctggaca agcatcaaaa atcaatgata
     7021 cgtgagctac tgtctttaaa ttcccattgg ccatcggacc gacataatcg atcgtatata
     7081 cctgatcatc aaaagcaatt gttccgcctg ctttcaaaga aaaaggttcg tcttttgata
     7141 cttcttgaat caccgaatat tttcgtaagg cagctgttgc tgttttatcg aacaaaatca
     7201 gcatgggttc ttccttgttt attgcttctg tcccaatttc tgtaatcttt gcctgaatca
     7261 ttgcactctc tcctttaatt gctttcaaaa acagtataac acattctcat tctttagccg
     7321 tgtggaataa ttatgtgata gaataaaaac gaaaactatt tcattacaat taggaggaat
     7381 cagctcatat gatcgaaaag ataattttag gaacctatac gcgtcgcgaa agtaaaggaa
     7441 tctatacgat cgaccttgat acagaaaaag aagaattagc caacctttca ttagctgttg
     7501 aagaaaatag ccctacctat gtagcaaaaa gtaaagctgg caatctttac acagttacta
     7561 gtgtggatgg attaggtggt gccggtgcgt acgataagga ttttcatttt ttgaatgcgg
     7621 taacagagtc tggcgctcct ttgtgttatg tagcggttga tgaagcacgt caattactat
     7681 acggggcgaa ctatcataaa ggtgaagtaa atgtctacca tatattagga gatggcgaac
     7741 tgaaagcagc agattcaata ttccatcaag aagcgacagg tcctcataaa aaccaagatc
     7801 atgcgcatgt ccactatacg gatctgactc ctgaccaacg attagtcgta tgtgatcttg
     7861 gtacagatcg tgtgtatact tatgatgtgt ccgaaaacgg caaattgaat cttgtaacta
     7921 ccttcactgc agagccagga acaggccctc gccatcttgt ctttcatcca aatggacaat
     7981 tcgcttattt atttggtgaa ttagacagca cagtccgcgt gctttcttat aataaagaaa
     8041 caggtgcttt tgaagaatta cagaaaatat cgaccttgcc agaagaattc gacggtgaaa
     8101 atggaggggc agctatccgc atttcaaatg atggcaagtt cctttatgct tctaaccgag
     8161 gacataactc gattgctgta ttcgcagttt ttgaagatgg ttcccacatt caaaacaagc
     8221 aaatcatctc aacagaaggg gattttcccc gcgatttcgc actagatcca agcaataatt
     8281 ttgtcgtgtg tgccaatcag aacaccgata atctgacatt atatcgccgt catcctgaaa
     8341 caggattact ggaattactg aaaaaagatg tctatgtacc agaatgtgtc tgtgtttatt
     8401 ttgatcgtta gtaagaaaag gagaatgaaa tgaatcagca agacgtatta gacagactac
     8461 gggaagaact aaatattcct ttctttgatg ggaaaatcga agataaagag tatagcgaag
     8521 aggaatatca gaaactcaaa gccgatttac agaactactt tgaacaatat gtacagaatg
     8581 tagaaaacta atcagctttc catactatct gaaaagaaca tcaggacaag acttttccgt
     8641 ctatcctgat gttcttttct acaaataaaa aaccgccgag atggttagac tcgacggaaa
     8701 aggagtattt actcgatttt gagtaagtac aaggttgttg ttggttatag gtatgtcatt
     8761 atcataaagg attttgatca ctctatcata aaggtaatat tacatttata ttagataact
     8821 ctagttttta ttgttatcaa agatttcttt tattttttca acaacaaatt agtcattttt
     8881 tggatggcag ctgtcaattg gtctgtcgct tggtcttgtt ttgcttcaga tgtattgaat
     8941 agatcttcat ctacccctga ttcaagatcg atactttgga tcatttcttc acagcctgct
     9001 acatatgaaa gataagcttt ttcaaaattt ttatgaatgc ccattgcttt tgctggtgga
     9061 cgcaaagtac tgactttcat aagcattgcc cgatattttt ttgttccttc ttcaaataac
     9121 aggatgatct cttcaagagt cgatttgtcc agttcacttg tttttccttc atcaattgcg
     9181 tttctgatca cttcatattt cggatgcatt ccttctccga ttgcttctgt ttccttgacg
     9241 atttgatcga tggactgcac ataatagcca atattcggta acatgttcgt tttcctactt
     9301 tctccattgt tgttattatt tattctactt gtttttatcc tcaaatgaca agtacttgaa
     9361 aaaatttaat ggtttctata cagctcgcca ttcttattcg tgctcaaaga aaccatctgc
     9421 taaactatat tgtttccctg cgacaatgtc atattggatc aattgatatt cgtcgtaaat
     9481 ctcttgttgc gagcgattga attgcgcttc tttataccac tgtccgtttt gatagtacag
     9541 ttctcgttca aacgccggaa gttcctgttc taatgataaa agcagctgat aaaagccatt
     9601 tgttgtctga ttcgtctgtt ccattagatt cgctgcaaaa taaaacggac tggtgattgc
     9661 atcgtgagtc gtttgctttt ctaattctcc tttactgtcg aacataagaa attgtgtttc
     9721 atgcaatgtt tgtttgtcat tttttgcttg gatcgtatct gaatagatcc ctggaagatg
     9781 atccccccaa aaaacaacta aagtccttct agaaagattc ttcaattctt ccgtgaactg
     9841 cttcagtgcc gtgctgctat aggaaatatc ttgcagataa ttctctaagt caagtgtccc
     9901 acttccatct gaagtttttg caggatagtc tagattatca tactttccat cgtagggcat
     9961 atgtgtctgc atcgtgacta aatgaatgaa ctgaggtgtt ttttcttcct tgagaagtgt
    10021 cattacttct ttgtaagctg actcatcaga aatatatgga ttattctcta tcgtatccgt
    10081 ataggtcatc gtacgctcgc tgatgaactg gtcaaatcct aatgtctgat aaacatcctc
    10141 tcttttatac atcgaagtat tgtatggatg gatcgctgtc gtctggtatg attgtgcgtt
    10201 taacgctgaa acgatagaag gaagctgatc catttttggt acaagcatcg tgtaaggggt
    10261 ggtgagttgc gcattaaata acgccataga aaaaccagtc aatgcttcaa attcgatatt
    10321 tgctgttcca ccgccatagt tttgagaaag catattgcca ctatatgttt gatcagctac
    10381 ttcataataa tctgccaatg gctcaccaga tacttctacg ccgtttaatc gactaggatc
    10441 tgagaaactt tcactcatca cgaacacgat gttcggcgac tcttcctcta cagttttatt
    10501 tttttcatcg gctagcttct ggtatttttc agtgatttct ttgatctttt ctttcgaata
    10561 tccttcaggt tcatccattg gttctacctt cagattgtac aaaaacccac cgataaaacc
    10621 agtgttatag tagttcattt tctggctgta aggtatccat agtgccgttt tattatatgc
    10681 ttttcgcaac aaattatcgg gattgttgaa attactgata taccctagca gtccaattgc
    10741 cacaagcagt gtcgttaatc ggatcaattg gaatcgcctt ccttttttca ggcttcgaaa
    10801 catgtaccag caaaacaatc ctatcacggt tcctgcggca gctaagatca ctgtaaacag
    10861 catagtgccg gtcatatctt tcagcaatcc gatttcagtg atcatcttca aatcgtctgg
    10921 ataaatcggt tcttgccgat aactcatttt catataattt gcaaaaccta atacaccgat
    10981 ggctacgaca tacgtaaggg aacctaaagg gacagatcct gctagtgcaa tcagaaaaat
    11041 aagaataatc agcaagacga gacaggctaa gaagaatttt tccgtatgcc aagaaaaagc
    11101 aaatttcaac gcaaggtcta cggatagatc attttgacac cactgcaaaa ataaattact
    11161 aaaaacaaca agaaaaaaga tactgatgat tgctgctgtt ccttttaaaa tctttttctg
    11221 tttggtcgaa agtctgctta tatatctttt caaatgcttg ctccttcatt tctatcaata
    11281 aaagaatgtt gcttctctta ttttagttgt acgaaattcg aaagtaaaga acggaaagaa
    11341 gaattaatga aatccaaata ttctctttct ttatggcctt tctggtttct ttaataagcg
    11401 gacctttcgt actccagaaa ttttttctag ctgttgtttc acttgattga gctgttcaaa
    11461 atcagtactg tctatgtcaa tcaatatgta gacatgctca ttcttatggt tacgcgatat
    11521 atctgcgatc gctagctcgt tctcattcag cagagaaaat atctctgcta atatattggt
    11581 tttcttttga taaaatatag tgaatcgaaa cggcgcttgg aaaaacattc gtgcggatgg
    11641 ataattgacc gcttctctaa ccgtaccgaa caaaagataa tcttttaagg cttcaactgc
    11701 caaacgtccg ctgtctgcca aagcttcctg agtcgatccc cctagatgag gcaacataag
    11761 tatacggtca ttttctaaga actcttcttc tggaaaatca gtcaagtatt tagccagatg
    11821 ttgtttttcc aatgcttgca taacagcata tttatcaaca atttcacttc ttcctacatt
    11881 gataagtaca gcatcttttt tcattttttc tatattcgcc tgatcgatca atccttttgt
    11941 atcttctgtc aacggcagca tgactacgat gtaatccgag gtagaaagaa ctgtttctaa
    12001 atctgcttgc cgaacataat ccaactgggc atcgcggata gaataaccca agacatccat
    12061 ccctaagcta tagcaactta gcgctacttt tgtgcctatc gctccaagac caagaagacc
    12121 gatggttttg ccttgcaatt ctcgtcccac ataagcactt cttttgtttt ctgcctgttc
    12181 taaaatatta ggacctgtta gtgtttgtac catacgactt gcttcgatta tcggacggga
    12241 agataagagc aaacagcaca aaacaagctc ttttactgca ttagcattta cacctggtgt
    12301 attcatgacg atcgtcccgt tttcagaagc tttttcaacg ttgatcgtgt tcaccccaac
    12361 accagctctt gaaattgcta gcaaatctgg tgtaagccat tcatctttca ctttcgtact
    12421 acgaataact attccttcgg ctggcaaatc atctattatt ttgaaatatt ccccttgtaa
    12481 cttttctatc tcttgttctg gaatagcatc aatcaaatga acgtgataca taaaaccccc
    12541 tccttactaa caagtatctc gaaatttctg caattagaca actgtttacc atttgacttc
    12601 ctagaagcaa aaagagcagt tagagcagag taggctgagt gataaaaata aggctatgac
    12661 aagttactgc cataagcctt attttcgaga gcaaaatatt gagggtgaga tatcttgata
    12721 aatggtgttc cgttagctga cattgataat aagactaaat ttcaaaaaag ttgaaaagca
    12781 aatttcgtca atctcttctt atttcttaaa actaagtggc tttttcacaa cctcttttta
    12841 tctaataacc actgtttcca actcaactaa ttttgcccac gtttcaattt gatctgttgt
    12901 tagatttagc gagacaacgg tatgatgacc gccacctgcc tgaatccatg aatggacacc
    12961 ttcatggaaa ttcggtttag ttttccataa cacacgtgca acaggtaaat taggtgcttc
    13021 agtcgttggt tcaaaggctt ctacttcatt gatcagcagt ttataatgcg taccgaaatc
    13081 tgccatcgat acgactaccc cttctcctgc tttcccatca aatacaagac gcgccggatc
    13141 ttctctatct cccattgata aaggagaaac aacgattctt ggtttcgtcg cagcaagcgt
    13201 cggatctact tccatcatat gggattccaa gattgcttct tggccactag ctagctcgta
    13261 agtataatct tccatgaatc ctgtatcttt ccctcgagcc atgattttca gcaaccgatc
    13321 aatcgctgca gttttccagt cgccttcccc agcaaaaccg tatccttctg ccatcaaacg
    13381 ttgtacagct agtccaggta gttgtttcat tccatacagg tcttcaaaat tagtcgtaaa
    13441 agcgttatag cctccttctt ctaagaaacg gcgaataccg atttcctgct gagcttgtac
    13501 ttttacatgt tcctcccatg tcttttcttc ataatcacca tagtcgaagg tatacaattc
    13561 cttgtactct tcaaataatt gatcgacttc ttcgtctgat acgcgatcga ttacttgtac
    13621 aagatctccg ataccaaaat aatcaacagt ccaaccaaat tgaatctgtg cttccacttt
    13681 gtctccttct gtgacaccca cgtttctcat gttgtcacca aaacgagcca cttttattcc
    13741 aaaactttca ttataggcta ctgcaacatc catccaatca gcaatctctt tttggatctc
    13801 aggtcgtttc cagtagccaa cgacgatttt attttgttta tttaatcgag cattgatgaa
    13861 cccatattcg cggtcaccat gagcggattg atttaaattc atgaaatcca tatcgatcgt
    13921 tttccaagga atgctttcgt tatattgcgt agctaaatga agcaatggtt tttgtagaag
    13981 ctttgtcccg cggatccaca tttttgctgg tgagaaagta tgcatccaag tgatcacacc
    14041 tgctacttct tctcgataat tgacctcttt catgattttt gtgatcgtat cagcagtaac
    14101 tgctaattct tgaaggcgga ttggataagg caattggcca ttttcattca gttcatcgac
    14161 catttcttgt gcatgtttct ttacttctcg cagtgcttct tccccatata aatgctgtga
    14221 tcctacaaca aaccaaaatt ctttttctcc aatattcaac ataattagcc actcctttta
    14281 tttgttttgt ccgtaatacg cattttttcc gtgttttcgt aaataatgtt tatctaaaat
    14341 ccgttgaggc agctcttctg aaaatgaatt gagctgtctg gtaaataaat tcatcttgca
    14401 tacttcatct agtacgactg cgttcattac cgcactttgc gcatctttcc cccaagtaaa
    14461 tggaccgtgt ccgtgtaaaa gtacccctgg aattgctaag ggatcaattt tgcgttcttt
    14521 gaatgtttct atgataactt ttcctgtttc atattcgtac ccgctgtcga tttcctgttg
    14581 tgtcaaaaat cgagcacaag gtaccgatcc ataaaaagta tctgcatgcg tcgtccccat
    14641 cgcaggaaca tccaatcctg cttgtgccca gatcgtcgcc catgtcgagt gagtatggac
    14701 gattcctccg atttgaggaa atgaacgata taaaacagca tgagtaggcg tatccgaaga
    14761 gggattgagt ttaccttcaa tgatatgatt gtccaagtcc acaacgacca tgtcgtctgc
    14821 tgtcatctct tcataactga ctccgctagg tttgataacg aaatatcgtg tttcaggatc
    14881 aaaagcgctc acatttcccc atgtatattt gacgagtccg tgtttaggca agtcaagatt
    14941 tgcttggtat acttcttctt tcagttgttc cagcattttc tatcactcct ttttcttgca
    15001 attgatgaat cgctgttttt tcaatgtcga tcccctcacg gtaacgtctc ataaatatct
    15061 gatagcctgc tatttcttct gctgaaggtg tgatggtgat tccttggtct tgtccaaaca
    15121 cgtcttctgc caagaagttt tctaaagact tcccatgtcg atccatcata taggctgcta
    15181 ataacgcgat tccccacgct ccaccttctc cggctgtgtc catcactgta actggtgcct
    15241 ccatagcaga tgctagaatc cgctgtccaa cttctggtgt tttaaaaatc cccccatgcc
    15301 ccactaaacg atcaatttgg acattctcag atttcaatat atccattccg attctcattg
    15361 caccaaaagc actggctaaa tggatccgca tgaaattcgc taaatcaaaa cggctacctg
    15421 gtgtacgtac aaataatggc cttccttcat ttactcctgt aatgttttca ccggaaaaat
    15481 aaccataaga aagtaatccc ccgcaatccg tatcgccttc tagcgctttg ttgaataata
    15541 ccgtgaataa tttatctctg cttatctcca tacccaaaga attggtaaat tcttcaaaaa
    15601 tactgaccca agcattgata tccgaagagc agttattcgt atgaaccatg cctactagtt
    15661 ctcctgaagg tgtagtgacc atgtcgattt ccggatggac attttctagt tctttttcta
    15721 atacgatcat agcgaaggcg gaagtaccag ccgaaatgtt ccctgtccct ttggctacac
    15781 tgttcgtagc gaccataccg gttcctgcat ccccttctgg cggacaaacc gggattcctg
    15841 gtctcaaatt tcccgtaggg tcgatcagat aagcccctga ttcgcttaac ttgccagctt
    15901 cttctcctgc tactagaact ttaggcaaaa tgtgatccaa gtcaatcgct acgtgcgctt
    15961 tttcgattaa ctgaccaaaa atatttttca tttcttgatg gtaggttttc gttgaaggat
    16021 cgataggaaa catgcctgat gcatctccta cgcctagtac tttctcacca gtaagctgcc
    16081 aatggacata acctgcaagc gttgtcaaaa aattaatttc tttcacatgt tcttctttat
    16141 tcaaaataga ttgatacaaa tgagcgatac tccatctctg cggaatattg tattgaaatt
    16201 tttccgttaa tattcgttct gcttcttcag tgattgtatt tcgccaagta cgaaatggta
    16261 ctagtaactg ttccttgcta tcaaatgcta gataaccgtg catcattgca ctgaacccaa
    16321 tcgacccgat tgtcgtaaga ttctcgccat agatttcgaa tacttctgct gccatttttc
    16381 ggtaactgac gcgcagtcct ttccaaatat cttctaatga ataggtccag ataccatttt
    16441 ctagtttgtt ttcccactca taactaccag cggcaatagg agtaaaagat gaatcaatca
    16501 atacagcttt gatcctagtt gatccaaact caatccctaa agatgtacga ccatctatga
    16561 catctaacat actttgcgca tttacttcct tcataatgat ttaactccct ccttctcgtc
    16621 aaacctgatc aaaccgtctt tttagtagcg cttacaatga ttattgtata cttttgtacg
    16681 tacatgtcaa gcagttattt acaagaatac aataaaaaac acttgttttg tacgtacata
    16741 agttataaaa tgaaattgct tacaaaatct gatcaaccgc cttctcgaaa ttctagaagg
    16801 agagaatact tatgaaaaaa gaaaaaaaag tttcctcatc gtttatttat ttttttggcg
    16861 catttggcgg cattctattt ggatatgata ttggtgttat gaccggcgcc ttaccttttt
    16921 tacagcatga ttggggtttg gcaggtaaag cttctttgat aggatggatc acgtcttcgg
    16981 tcatgttagg tgcgatcctt ggcggctctt tatctggtct tctttctgat aagcttggac
    17041 gacgaaaaat gattcttctt tctgcactta tttttatggc tggttctgtc ctttctgcat
    17101 ctgcccctca caatggttct tattttctga ttgcagcaag gattcttcta ggtttagctg
    17161 ttggcgctgc atctgcccta gttcctgctt atatgtctga aatggctcct gctagactaa
    17221 ggggaagatt gtcagggatc aatcaagtga tgattgcttc tggcatgctc ctttcctatg
    17281 ttgcagatta tttgctaaaa gggttgcctg aaacgatggc ttggcgcgtg atgttagggc
    17341 tcgctgctgt ccccgcgctg attttatttt tcggtgtttt agctctacca gagtcaccgc
    17401 gttttcttat gcaatctggc cgcttggaag aggcaaaaag agtattgaac tatattcgga
    17461 caccaaaaga agctgaacag gaattcgaac aaatccagct gaatgtcaaa caagaaaaga
    17521 caaccggaac ttcttggcat actttgtttt tagaaaaata tcgttcctta gtcattgccg
    17581 gtattggtgt agcagtattc cagcaatttc aaggagctaa tgctattttt tactatattc
    17641 cgctgatcgt agaaaaagcg actgggcatg cagcgagcga tgcattgatg tggccgatca
    17701 tccaaggaat cattttagtt gctggttctt tgctcttttt ggttattgca gacaagttca
    17761 atcggcgaac tttattgaaa ataggcggta gcgtgatggg actttctttc atcctaccag
    17821 ctgttttagg aactgtactg gatgctcata caaattcgtt gttgattctc ttgtttttat
    17881 gtatttatgt cgcattttat tcttgcacat gggctccact gacatgggta atcgtaggcg
    17941 aaatattccc actagctgtc cgcgggcgag catctggcct agcctcttct tttaattgga
    18001 ttggttcttt cctagttggc ttgctttttc ctgtgatgac tgcttctatg tctcaagaaa
    18061 tcgttttcgg catttttggg atcatctgct ttttaggggt tttatttatt caagagatcg
    18121 taccagaaac tcgtgggaaa tctttagagg aaatcgaaca aagtgcatct aaaaaaactt
    18181 atcctaaacg tatttctatc catcattctt aaagaggttg tgacagaagt ggtcagcttc
    18241 aagaaataag aaggaaccgc ttagtttcca gtcacaagga acaatttcaa aaaacagctt
    18301 ctcatgtttg atgaatattg tgacttgtga ccagttggta ggagaacacc gtttagtgga
    18361 gaggtggtga aagaaacgtt cagccttgag cgatgacaaa agctctcaca aacattgcga
    18421 cgagtaaccg taggagcaat aagaggcggt aaaaacccgt ctcttatttt tcgttttctt
    18481 tcactgtttc ccgttcaact agtacaggtt catagagaat atttggctgt gcctgtcctt
    18541 tttcaatcgt tgtgacgatc caccgtgcgg catcttgtcc caacttttct tttggatgcg
    18601 tcaatgttgt caatttttta ttccctaaat ggcttaaaga agaatcgtct gttcctacga
    18661 tcgataggtc atctggaata tgatagccat tttctgtcaa cttatctagc attaaactag
    18721 ctatttgatc gttatagcaa atgatgccat ctaaagcttg ttcttctttc aaccgttgta
    18781 ctgttttttc agcgattgtt tctcgtgttt ccgtggtgaa agtaatgata tcttccgtca
    18841 aaaaacgcac attatactgt tcacacgcgc tgataaaacc tttcatcctg tattttcctt
    18901 gaagatcgtc gatttttgtg atgaacaaca gacgctgatg tccttttttt atcaaatatt
    18961 ctgctgccat aaaaccgact ttcacatcat ctaaacaaat ataagaggca tttaactctt
    19021 cataagcagc attgatcatc accatcggaa tgccttgttc tctcaaagca acataaagtg
    19081 ctaagtttgg gttatactga ttacttttcg taggctcaac gatcaaacca tctaccccat
    19141 aagcaatcat tttcttcaaa cactctttct ccaattcgtg atcgttattc gtactggcta
    19201 aaagcaatga atacccagat tctcgtaatt cattttcgat tcctcggata atcgacggaa
    19261 aaatataatc agagagatat gtggtgatga caccgatcgt ttttgttctg ttcaatggtg
    19321 ttttttcttt cttatagctg tcatctacat atgtgcccga ccctttttct ttacgcagat
    19381 agccctcatt taccaacaca gcgattgctt gccgaatcgt atgtctgctg acgtcatata
    19441 tttcttgcag cttgaattct gagggaatgg ttgtatttac ttcgtattcc tcacgaagga
    19501 tcttttgttt tatctcttct gcaatatctt gatattttgc catttgcctt gccccttttt
    19561 atttgtacgt acatatatca taacgagtct gcaaggttta ttcaagcaaa aatcaaggtt
    19621 tcttgtgctc ataaggcata cggaacaggt tcaccaaatt ctggaaatcc ttgctcattc
    19681 cacgtaaagc attgcgcgtt cgcgtgccga ttaggattgt ctaacggatc ccctttttgg
    19741 ttcttctctg gtcgagcatg atagatcaaa atatcaaagc acccgtcttc tgaaaccgta
    19801 aacgagttgt gaccaggacc aaattgctta tttttttcag aagaaacgaa tactggctcc
    19861 gataatttat gccatgattc ttttaataaa agattcttgt gctcatcagc ccacagcagg
    19921 cccatggcat agttttcatc tgttgcacta ccagaatacg taatgaatat tttcccattg
    19981 cgagtaagga ctgctggacc ttcatttact gaaaaacccc gtttttccca ttcatactca
    20041 ggtatagaaa ggaggatctg cttccccttt aacgtccaag gattatccat ttcggataga
    20101 taaagattag aattcccagt aatttcagga tcttgttggg cccacacata atatagcttt
    20161 ccttgagaag agaaagttgt tccgtctaaa ctgaaagttt cccttttcgt ttttatttgt
    20221 cccttttcta tccaatctgt attcattgga tctatatgct gattttcaag tacgaacatg
    20281 cggtgatgat gagattcatc acgtatagtt ttatgattgc ttgcagcaaa gtaaatatac
    20341 cattttccat tgatataatg gacttcaggt gcccagatca attcactcat cggtccttct
    20401 tcatgagcct gccatacaat caggcttgca gcatgttcta attcattcag tgactttgcc
    20461 cttcttaatt caatcacttg gtagcctgga accgatcctg taaaataata ataaccatct
    20521 gtatgtttat atacccaagg atctgcccgt tgccgaacga taggattatt ataaagtgtc
    20581 ttcatcataa caaatttcct cacattttct ttattctaat tacattccaa gagtattttt
    20641 ctatccctac atttaattcg tcatgttttt ctattgaaaa atagtttatt tcaggtttta
    20701 ttttttctgg ttcttcacgt gtatttttgt ccaacagatc gctactggtt aatgtgtagt
    20761 gctctccttg atctagcaaa ttgaaatcat gttcaatcgt tatcttttgt tgttcatttg
    20821 tgcgattgat caagaaaata gtcagctgat tgccgttatc tacgatcaca ctgtccacat
    20881 aaggaacatt ttttatcttt tcacaatcat aaacagggca aatgatttgt tgtttcaata
    20941 cttgaccttt agcatatttc gataagtgtt gcatcacata ataagttgga ttcctccatg
    21001 cttgtccttt agcatctgtc agtattaaag gtattgtgtt tactaacaat gattgacaac
    21061 taatctttac gcgatctgcg tgtcgtaaaa tagccagccc taatgaacca aataataatg
    21121 tatcttccat agtaaagtaa caccaatcga tgggactacc gacttcaaaa tccctatgtc
    21181 ttttttccgg ttgggcatgt acattccatt catcaaacgc tagatacatt gttttctcac
    21241 ttctcaaata tccttttaca taatcacaag tagcaacgat ttcttcaatt tgacgatcga
    21301 atcgataagc ttttgcaagg tatgtcgctg tatcatcacg ctcattttta ggaggtttag
    21361 tcaagtcttc ttcatcatac ttatcaatat agttatgcaa agctaaataa tctacattct
    21421 cataagcttc ttctaatgcg atacgatccc attcagggta cgatggtaaa cgtggcgtag
    21481 agcttccaac taagattgtt tcaattgaat catccactaa cttcatgact tttgaggctt
    21541 catttgctaa cctaccatat tcataagcac tcttagcccc cacttgccat ggaccgtcta
    21601 gctcattccc taaacaccat aatttaacgc caaaaggctt tttttgccca ttttttattc
    21661 ttaaatcact ccagaaggtt ccttttggaa aattacaata ttctactaaa ttttttgctt
    21721 catcaattcc ttttgttcct aaattcaccg tgaatatcgg tttcgcttgt acatgactga
    21781 tccatttaaa aaattcgtgg agtccaaaag aatttggttc tatacctcgc catgctaaat
    21841 caattttttt tggtcgttgt tctttcctac ctacagtatc cacccaatca taaccagatg
    21901 taaagtttcc tccaggatag cgaatgacac ctaattctaa ttcctttaca taagagagga
    21961 catcttgtct aaacccgtca ccatcagctg tttcatggcc cggttcataa atccctgtat
    22021 agacgacact tcccatatgc tcaacaaatg agccaaacag ccgagaactg atctctccta
    22081 ccgtaaaatc ctctctgatt tttactttca cttcttcgtc caacagcttt ccctaccttt
    22141 catttcctat agtcaagata atagatacaa gtttccatac gccaccagca aagataaaaa
    22201 taaaaggagg gaaaagaaat atgagcagta caaatcctaa taacaatacg caacaaagaa
    22261 tatttctcaa aaaatcaaaa aatactttcc atgcactttg ttcaaaaatt tgtaaaaatg
    22321 tgatatcttt gttttcaga
//
LOCUS       DANKKG010000037        21694 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000037 DANKKG010000000
VERSION     DANKKG010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.037"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(27..602)
                     /locus_tag="PW004_001169"
     CDS             complement(27..602)
                     /locus_tag="PW004_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL2726156.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(607..1581)
                     /locus_tag="PW004_001170"
     CDS             complement(607..1581)
                     /locus_tag="PW004_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleosidase"
                     /protein_id="HDL2726157.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIASALKVNPNIGKHMERFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEEAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(1605..1649)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1864..2832
                     /locus_tag="PW004_001171"
     CDS             1864..2832
                     /locus_tag="PW004_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL2726158.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     gene            2929..3222
                     /locus_tag="PW004_001172"
     CDS             2929..3222
                     /locus_tag="PW004_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDL2726159.1"
                     /translation="MRSAAYGNQRRKNRLALFNDEQQEIGEMTWSDAGPDIMIVDHTF
                     VDPAYRGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            3340..3576
                     /gene="secG"
                     /locus_tag="PW004_001173"
     CDS             3340..3576
                     /gene="secG"
                     /locus_tag="PW004_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HDL2726160.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            3679..4434
                     /locus_tag="PW004_001174"
     CDS             3679..4434
                     /locus_tag="PW004_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL2726161.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQVAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            4437..6794
                     /gene="rnr"
                     /locus_tag="PW004_001175"
     CDS             4437..6794
                     /gene="rnr"
                     /locus_tag="PW004_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HDL2726162.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            6816..7280
                     /gene="smpB"
                     /locus_tag="PW004_001176"
     CDS             6816..7280
                     /gene="smpB"
                     /locus_tag="PW004_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HDL2726163.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            7582..8301
                     /gene="atpB"
                     /locus_tag="PW004_001177"
     CDS             7582..8301
                     /gene="atpB"
                     /locus_tag="PW004_001177"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HDL2726164.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            8354..8569
                     /gene="atpE"
                     /locus_tag="PW004_001178"
     CDS             8354..8569
                     /gene="atpE"
                     /locus_tag="PW004_001178"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HDL2726165.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            8660..9184
                     /gene="atpF"
                     /locus_tag="PW004_001179"
     CDS             8660..9184
                     /gene="atpF"
                     /locus_tag="PW004_001179"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HDL2726166.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            9171..9713
                     /locus_tag="PW004_001180"
     CDS             9171..9713
                     /locus_tag="PW004_001180"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HDL2726167.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            9736..11292
                     /locus_tag="PW004_001181"
     CDS             9736..11292
                     /locus_tag="PW004_001181"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HDL2726168.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            11306..12208
                     /locus_tag="PW004_001182"
     CDS             11306..12208
                     /locus_tag="PW004_001182"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HDL2726169.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            12231..13637
                     /gene="atpD"
                     /locus_tag="PW004_001183"
     CDS             12231..13637
                     /gene="atpD"
                     /locus_tag="PW004_001183"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HDL2726170.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            13651..14073
                     /locus_tag="PW004_001184"
     CDS             13651..14073
                     /locus_tag="PW004_001184"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HDL2726171.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            14542..14772
                     /locus_tag="PW004_001185"
     CDS             14542..14772
                     /locus_tag="PW004_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HDL2726172.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            14869..16170
                     /gene="murA"
                     /locus_tag="PW004_001186"
     CDS             14869..16170
                     /gene="murA"
                     /locus_tag="PW004_001186"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDL2726173.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            16183..16359
                     /locus_tag="PW004_001187"
     CDS             16183..16359
                     /locus_tag="PW004_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDL2726174.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            16494..16790
                     /gene="yidD"
                     /locus_tag="PW004_001188"
     CDS             16494..16790
                     /gene="yidD"
                     /locus_tag="PW004_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HDL2726175.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            16854..17321
                     /locus_tag="PW004_001189"
     CDS             16854..17321
                     /locus_tag="PW004_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2726176.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            17406..18290
                     /locus_tag="PW004_001190"
     CDS             17406..18290
                     /locus_tag="PW004_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL2726177.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLIGAAANYLAVRQGEN"
     gene            18404..18640
                     /locus_tag="PW004_001191"
     CDS             18404..18640
                     /locus_tag="PW004_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HDL2726178.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            18728..18808
                     /locus_tag="PW004_001192"
     tRNA            18728..18808
                     /locus_tag="PW004_001192"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18762..18764,aa:Tyr,seq:gta)
     gene            18815..18886
                     /locus_tag="PW004_001193"
     tRNA            18815..18886
                     /locus_tag="PW004_001193"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18847..18849,aa:Gln,seq:ttg)
     gene            19012..19182
                     /locus_tag="PW004_001194"
     CDS             19012..19182
                     /locus_tag="PW004_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726179.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            19402..20109
                     /locus_tag="PW004_001195"
     CDS             19402..20109
                     /locus_tag="PW004_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2726180.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRRDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(20168..20812)
                     /locus_tag="PW004_001196"
     CDS             complement(20168..20812)
                     /locus_tag="PW004_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HDL2726181.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKNFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSANHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            20939..21226
                     /locus_tag="PW004_001197"
     CDS             20939..21226
                     /locus_tag="PW004_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726182.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            21334..21654
                     /locus_tag="PW004_001198"
     CDS             21334..21654
                     /locus_tag="PW004_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2726183.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIA
                     LIGE"
BASE COUNT         7180 a         3772 c         4676 g         6066 t
ORIGIN      
        1 tattttgcta atccagctct gtttattcag cctatttttc tttttgaata tatcaagcgt
       61 tgtggcaaca tagtgatgac taatgaaaca acgacgatag acaacaaacg atcaaggtag
      121 tctgtacctg cctgtaccaa gatcacactt gttgtctgac ttaaacctaa cccatgtaat
      181 agctgaacga tcatactgga tcctgaggag gtgatcccgc caaataactg aacggtaatg
      241 atcgaagaaa cgatcgttcc aggtaccgac aaaatcaaag cgagtaataa cagtttcaag
      301 ctgtttttat ctttgccgag atatcttggc aaaatcaatc cagcaagcaa gcctgtgaga
      361 atttggattg gcgaataata cagagaaaaa atatctgtag tcactccact taaaataccg
      421 ctggacaatc ctgtcagcat tccaggtaca ggcccaagca atgcaccagc aaaaatcgtt
      481 ccgatagaat cgagatagat tggcaaacga agccataaag caatcgttcc tccaatataa
      541 ttgatcccaa tacacaaagc catcactgta agtacgcgag ttgccatttt gtttttattc
      601 attttgttag cccatcttta acctttccaa atctttcata atcgtttctt cttgtgcatt
      661 taaaagaacg gttaaaaact ttcggaaaaa aagacgagta tctacttttg tcataatttt
      721 actgttagca ggtttctgcc aaaattcata acgatcaacc aatgtttgtc cacgacttat
      781 tccttgagtt tccacagcag tgaagctttt gaaaccttca cagatggcct cctcgataaa
      841 ataagcaaca gctaacggat cattgatgac acatcctaaa attctttctt gtttccagtg
      901 gaagtcaaaa taaaaacgag tgatcgcttt tagatactct ccctcttcag gattcatttg
      961 acagcagtat tcgaggatag tcggtgtcaa gacgatttct cttgtcacat ctaacccaac
     1021 catttctatc gtttgtttga gattctcgaa tacataagaa gctgcttcag gatcacacca
     1081 gtaattgtat tcagcaactg gcgaacagtt accatggctt ttgtaagttc cacccatcga
     1141 gacaaaacgt tccatatgtt tcccaatatt tgggttgact ttcaaagcag aagcgatatt
     1201 ggtcaaagga cccaaagcaa tgactgatgt atttgtcttt tctttgaaat aatcagccaa
     1261 aaaatcaacg gcatggagag gttctgcctg ttttctcaag atcatcggga aatttgtttc
     1321 ccctaaacca tccataccat gagtgtcttg tgcactgatg aaaggttgct tcaaaggttt
     1381 ttccattcct tgataaacag gaatgtctaa acgatcacag cgttccaggc atttcagcac
     1441 attctgcgta cctatatctg cagggacatt cccacaaact acggtaatag caactacttc
     1501 gatatcaggg gatttgacag ctaatgataa agctaaagtg tcgtcgatcc ctggatcgca
     1561 atcaataatt acttttcgca ttatttccaa tctcctttct ttagagatac ttagtttttt
     1621 attctgggaa gtttccgaac cagtccaaga cgagcgtctt gattataatg cacgaaatta
     1681 gtatactggc ttttattgcg aatgtaaatg ctctcttaca agtaaatcta ttgaggagaa
     1741 ctttaagtaa atgatacaat gaaaaagaaa tggaatttac gtgtgccgaa aataaagaat
     1801 cctatggatc ggtacattgg ataatagata gattatagtt tggcagaaag cggcgggtgt
     1861 acaatggtta ttttttattt cattcttatt ctgataatta tagggatcag ttaccatttt
     1921 ctttcttatt tttatgcaaa gaaattagta agagcgggag tacagagagg atatgactgg
     1981 tatgaagaca ctggtcatcg tttgatggac cctgctgcag tcacgtcagt agaaaaaaaa
     2041 cgacaaaaat tagaagaaga gtgtgaaagc ttttgggagc agggaacacc caaaacaatc
     2101 aaaagttatg acgggttgaa actagctggt cagatgttca tccatccaaa taagcagaat
     2161 aaatgggtca tttgcgtaca tgattaccga agcacaggga aaagagacat gtctcatata
     2221 ggtaaaagat atgcagagaa aggattcaac gttcttgtac ctgatttacg tgcgcacgga
     2281 gaaagtgaag gagaaatcat cggaatggga tggctagatc ggctagattt aattgcctgg
     2341 atccaactca tcttagatga acagccgaat gcgtccatta ttttacacgg cggatcaatg
     2401 ggagcttcta cgatcatgat ggcaagtggt gaaaaacttc catcagcggt caaaggattt
     2461 attttagaca gcggatatgt ttctgtttat gcagaattcc gatatatgtt aagtaaaatc
     2521 actgtctttc ctaaaaaaat ggtcatgaga tatgcgaatc attatgccca gaaatacgcc
     2581 ggttactcct taaagcaagc atcagctacg agacagcttg gcagcaatca tctgccttta
     2641 ttagtcatcc atggtgaaag agatcatttt gtccctacag aagcagcgta cacgattcaa
     2701 aatgctacgg caggggataa agcactgctg cttgttccgg aagcagaaca cttggaagca
     2761 tcgatgaaag atccgaaaac ctattggaca gtgatttttt catttattga acaaagaata
     2821 acgatgaatt aattttctct tcttttttgt aagtaattcg aggataaata attgtttata
     2881 gatcaggaga ctgctaaaat aaggtaggaa taaaaaaata tcagttattt gaggagtgcg
     2941 gcatatggaa atcaaagaag aaaaaatcgt cttgctttat tcaatgatga acaacaagag
     3001 atcggagaaa tgacatggtc agatgcgggc ccagacatca tgatcgttga ccatacattt
     3061 gtagatccag catatcgtgg tcaaaagtta gctgaaaagc tagtgtatac tggagtagaa
     3121 ttagcaagac gagacgggaa aaagatcatt cctttatgcc cttatgcaaa aaaagagttt
     3181 gaaaagaaac cagaatatca agacgtctta cgtcagtcat aagaataaca gctgaaagtt
     3241 ttgaactttc agctgttatt ccatttatat agagttgtca cggactttta tctatgataa
     3301 agttaaagag acgatatctt ttgggaagga ggctgcgata tgtataattt aattttggga
     3361 atcgtgatcg ttttatcgat tgttatggtc attgcgatta tgatgcagcc aagcaagcaa
     3421 aatagtgcag cgagtgcatt taccggcggt gcggatcaac tattcggcaa acaaaaagct
     3481 cgcggctttg aagctgtcat gcagcgatca actgcagtta tgggagctat ctggatgatc
     3541 ttattatttg tcctagcata tttatcttcg aagtagttga ataagcctct cgcaaaacgg
     3601 cgggcggctt tttttattgc aaagagaacg cttaagaaga agttatgaaa gaaactatgg
     3661 taaaatgagg cgaaagaggt gagacgagtg agacaattac caaaacccct ttatgcaaaa
     3721 catggaaagc gagcggtttt gctgcttcac gcttactcag gaagtccaaa cgatgttcga
     3781 atgctcgcac gatttttgga aaaagcagat tatacagttt atgcgccgct ttttaaagga
     3841 cacggaacaa tggagccata tgatatcttg caagaaaaag cagaaagctg gtgggcagac
     3901 actaaaaaag ccatccattt tttacagtct gagcagtttt ctgatattgc cgtattagga
     3961 ttatcaatgg gcggcatctt tgctgtacga gcactagaag aagaatcagt gattggcggc
     4021 ggttttttct gttcgccttt gtctcctgta aaaacaaacg tgcctgaaaa ctttgaaaaa
     4081 tacgtacgtc aagtgctgaa gacagcaggt aaatcagaga aagaaatcaa tgaaaaagca
     4141 gttgcttacc gctcgttagc tgaacaacaa ttaatggata tacaagatca ggtagcaatc
     4201 gtagaaagta ggttgtcaga tatccaacag ccgatatttt tagctcaagc tgggaaagat
     4261 gaaatgatcg accctaatgg cgtatttgaa actgcacgca agttgagccg tcagcgagtg
     4321 acacttcaat ggtatcctga aagtggtcat gtgattacag ttggtacagc aagacgcgaa
     4381 ttagaaaaag acgtcttgga atttttagaa aaattacctt ggaatgagga ataaatatga
     4441 caaaagaaac gataaaagag aaaattctct ttttcatgga aagcagtcgc aaacgcagtt
     4501 tttctatgga agaaatcgct gaaggtttag ggttccaaaa aagcgatgat tttaaattac
     4561 tagtacaaac gattgctcaa atggaaagag aacagagtat agttttcaat aaaaaaggaa
     4621 aagtgaaact tcctttaaaa ccaatcctga tcgaaggaac atttcgagca aatgaacgag
     4681 gatttggatt tgtaacgatt gatcccgaag aagatgacat ctatatccca aaagaagcga
     4741 ctggttttgc aatggatgga gacaccgtag caatcgatat catgaagcga gcaaatacag
     4801 atatggatcg cggagcagag gggaaagtag tcgagattcg agaccgggcg actactcagt
     4861 tagcagggga attcgttgct tattcagaag aagaaataca agaaacagat ctttacggcg
     4921 ttgttatccc gaaagataga aagctcaacc aatttaaagt atatgcagca gcagagggag
     4981 ttcgtccagt tgacggaagt attgtgctga tcgaactgac ccattaccct gaaaaagggt
     5041 atgcgactag tttagaaggg ataatcaagc aagtgattgg tcataagaat gaccctggca
     5101 tggatatcct ctcaattgtc gtggctaatg gtatccctac gaaatttcct gatgatgtat
     5161 tgtctgaagc agatgaagtt ccagatcaaa tcagcgaaaa ggatataact ggacgtagag
     5221 atttaagaaa tcagctcatt gttacgattg atggagaaga tgcaaaagat ctagatgatg
     5281 cagttacggt taaaaaatta gataacggta attattttct tggagtacac attgcagatg
     5341 tttcttacta tgtgacacaa ggcagtcaat tggacagaga agcatatgaa agagggacaa
     5401 gtgtatattt gactgatcga gtaattccga tgatcccgca acgtttatcg aatggtatct
     5461 gttcactgaa tccccatgtt ccaaggctga ccatgagttg cgaaatggag attgatccaa
     5521 atggacaagt agtctctcat gatattttcc caagtgtgat ccaaacaaca gaacggatga
     5581 cctacacagc tgtcaatcaa atcttagaag atcaagacga agaaacgatg caacgctatg
     5641 ctcagctcgt acctatgttc caagaaatgg gtgaattgca ccgaatcctt gaaacgatgc
     5701 gaatcaaacg tggcgcgatt tcttttgagg atcgcgaagc gaaaatcctt gttgatgctg
     5761 aaggacaccc aaatgacatc cagctaagaa gcagaggaat tggtgaacgc ttgatcgaat
     5821 ctttcatgct tgcggccaat gaaacggtag ctgaacatta taataaatta aatctgccgt
     5881 ttatttaccg aatccatgaa cagccaaaag aagaaaaaat gcagcgcttc tttgattttg
     5941 cttcagcttt gggcattctg gtccgaggaa cgaaaggaac gatcacacca aaagatttac
     6001 agcatgtgat cgaaggagta gaagacaaac cagaagctgc agttatcaat actatgttgc
     6061 taagaagcat gcaacaagca cgctattcag aagacaattt tgggcattat ggcttagcgg
     6121 cagattacta cacacacttt acatctccta ttcgacgcta tcccgatcta atcgtccatc
     6181 gtctgatccg ttcttacagt gaagataaaa gtgaaagcaa tcaaagttcg tgggctgcgg
     6241 aacttcctga aattgcagat catagttcta agatggaacg tcgtgcagta gaaacagaac
     6301 gtgaagtaga ttccatgaag aaagcagaat tcatgatgga caaagtggga gaagaattcg
     6361 atgggatcat cagctctgtt gtgaaattcg gcctgtttat cgaactgccg aatacagtag
     6421 agggattgat ccatatcaat gaattgaaac aggactactt ccattttgtt gaaaatcatt
     6481 tagctcttgt aggtgaaaga actggtctga cttttaaaat cggacagaaa gtgcgagtaa
     6541 aagtagtcaa agctgatcca gatgaacgag cagtagattt tgaacttgtt tccgcagaag
     6601 aggttacagc gttggaacgg ccaaaaacac gaaatggaaa atcaggaaaa cgcgaaggaa
     6661 aacaagcagg aaaacgccca gaaagaaatg gcaagaagaa caaacgatat agcgatagta
     6721 agcaaacgtt cggtaagaaa aaagggaaga agccatttta caaaggtgtt aaaaagaaga
     6781 aaaagaaaaa ataaaatgag ttggaggaaa cagctatgcc aaaaggcgag ggaaaattaa
     6841 ttgcacaaaa caagaaagct cgccatgatt attcgatcat cgacacgatg gaagcaggga
     6901 tggtcttgca aggaaccgag atcaagtcga tacgaaacag ccggatcaat ctaaaagatg
     6961 gatttattcg cgtccgcaac ggagaagctt tcttgcataa tgttcatatc agtccttatg
     7021 aacaaggaaa tatttttaat catgatccgt tgcgcacgag aaagttatta ttacacaaaa
     7081 aacaaatcat ccggcttgaa aatgaattga aaaatactgg aatcactgtt gttcctttaa
     7141 aagtctatat tcgtaacggc tatgccaagg tattgattgg tctggcgaaa gggaaaaaat
     7201 cttatgataa acgggaagat ttgaaacgaa aagatatcga tcgacaaatt gatcgaacat
     7261 taaaaaattt ctctagataa tttcattatc ctttttcgca tattcggtgc ttttgctttt
     7321 acttaatgat aaaatttgtt agcatttaat ctgataaaac atgattatca taggtttttt
     7381 cttagatcag gagcaccgtg tataaagaaa aattcgcttc gtttttgact gaaataaaaa
     7441 agtgtctaat tttcatgaaa atgttttgaa ttttctgttt catggtggta tgataccgtg
     7501 tggttagaaa ccgattggaa aaaaccagat acgcacatga attgatttta tgtgaatttc
     7561 ggggaaaaga ggtgaaatgg attggatgaa cgctcgctaa cgttccacat tggaccaatt
     7621 tggtttgatg gtacagtttg tataatggtg ttactgacct gtttgattgt tttcttctta
     7681 gcttactttt ttacaagaaa tcttaagttg aaaccaacag gcaaacaaaa tgcgatagaa
     7741 tgggtcattg attttagtcg aggaatcgta acagataatc tgcctagaaa agaattgagc
     7801 aatttccatt tgttggcatt tacattgttt ttatttgttt ttgttgcaaa caacatcggt
     7861 ttagtaacaa aaatcgtttt acctaacgaa acaacacttt ggaaaagccc gacagcagac
     7921 ccgtttgtta ctttaacttt ggcatttata atgatcttac ttactcactt attcggggtg
     7981 aaaaagttag gcttcaaagg atatttcgta aattcgttct tgaaacccta tagttttatg
     8041 tttccgatga aactgattga agaatttaca aacttattaa cacttgcttt acgtctttat
     8101 gggaatattt atgcggggga agttttgcta accttgattg caaaaatgat gacaagctta
     8161 ggctggttct cattaccatt agcaattcca ttagagatgg tctggatcgc gttctccttg
     8221 tttattggga gcatccaagc atttgtcttt gtcactttgt caatggttta tatgagccat
     8281 aagattgaag tagaagaata atactcggtt aaaaaacaaa ctactattta tctattttag
     8341 gaggaaaaca attatgaatt atatcgcagc agcaatcgca atcatgggag cagcaatcgg
     8401 agccggttat ggtaacggtc aagttatctc taaaacaatc gaatcaatgg ctcgccaacc
     8461 tgaaatgtct ggtcaattcc gtacaacaat gtttatcggg gtagctctag ttgaagctgt
     8521 tcctatctta ggggtagtta tcgccttgat tctagttttt gccgtttaat tgaatcagta
     8581 ttaaggcaga aacaacatcg tttgttactg cttttttaca tggaaactag aaatactcca
     8641 aagaaaggaa ggctcgccta tgctgaatca attggcaatt gcagaagttg gcaatgcgat
     8701 gttaggtaat atcatcgttg tcagcggctc atttttgata ctactggctc tcttgaagca
     8761 ctttgcttgg ggaccaatca gcgatatttt gaaaaaacgt gaagacaaga tcgccaatga
     8821 tttagattct gcagaacaat ctcgcatcaa ctcagcgaaa atggaacaag aacgcgaaca
     8881 acaattgtta gcctctcgtt ctgatgcagc tgatatcatc aaaaatgcga aagaaagtgg
     8941 agaattaagc cgccaaaata ttttgaagga tgctcaagaa gaagcagctc gtctaaaaag
     9001 caaagcccaa gctgatatca ctgtagaacg tgattcagcg ctgaactctg taaaagacga
     9061 cgttgcagaa ctctctcttc aaatcgcgga aaaaatcttg aacaaagaat tatctccaga
     9121 aatgcatgaa tcattaatca atcaatacat tgaaggtcta ggttcttcaa atgaaactag
     9181 ataaatatac agtaggaaga cgttacggga aagctttatt tgaactggcg atcgattcaa
     9241 atcaagcaga agaaatctat caagatctcg taagtttacg tcaaatctat gaacaagtac
     9301 ctggattagg gaatatgtta agtgatgttc gtctagaacc tcatgaaaaa cgagaaatca
     9361 tggacaagct tgtcagcggt tatgatggga tcgttaaaaa ctttttagaa gttgtttatg
     9421 agtataaccg aatggatgac cttttgttca tgatcgaaga atatgaacgt cgatatgatg
     9481 aacatcaagg gttactattg ggaagtgtca caacagcagt cccactttca gacgaacaac
     9541 gaatacaatt agaaaagaat gttgcaaaaa caatggacta tcaatcagtt gaattaaaac
     9601 aaatcgttga ttcttctatt attggtggcg cgattgttga agccaaccat cgagtaatcg
     9661 acggaagcat tcgcacgcaa ttagaaaaaa tgcgtaatca attaaacaga tagtacaaag
     9721 aggtaggtga atcgaatggc catcaaagca gaagaaatta gtgccttgat taaagaacaa
     9781 attgaaaatt atcaaaacgt gctttcagtc gaagaaatcg gtaccgtaac ttatgtaggt
     9841 gacgggatcg ctcgtgcaca tggtttagaa aacgcgatga gtggtgagtt gcttgaattt
     9901 tcaaacggct catacggaat ggcgcaaaac ttagagtcaa atgatgtagg tatcattatc
     9961 cttggcgatt tcgagtccat tcgtgaagga gataaagtaa aacgaacagg taagatcatg
    10021 gaagttccag ttggagaggc cttgatcggt cgggtagtca atccgctagg tcaaccaatc
    10081 gacggactag gtgaaatcgt tactgacaaa gctcgtccag tagaagcgat ggcaccaggt
    10141 gttatgcaac gtaaatctgt taacgaacca atgcaaacag ggctaaaagc gatcgacgcc
    10201 cttgtgccaa tcggacgcgg acaacgtgaa ttagtcatcg gtgaccgtaa aacagggaaa
    10261 acttctattg cgatcgatac gatcatcaac caaaaaggtc aagatatgat ctgtatctat
    10321 gtagcaatcg gacaaaaaga ttctacagtt cgtacacaag ttgaaacatt gaaaaaatat
    10381 ggcgcaatgg attacacaat cgttgtgaat gccggtgcgt ctcaaccagc accattgctt
    10441 tatatcgcac catatgctgg tactgcaatg ggtgaagaat tcatgtacaa cggtaaacat
    10501 gtattgatca tttttgatga tctttcaaaa caagctgttg cttatcgtga actttccttg
    10561 ctacttcgcc gtccaccagg tcgtgaagct tatcctgggg atgttttcta cttgcactca
    10621 cgtttattag aacgtgcagc aaaattaagt gatgatttag gtggcggttc aatgactgcc
    10681 ttaccatttg ttgaaacaca agctggagat atctctgctt atatcccaac aaacgttatt
    10741 tcgatcactg atggacaaat cttcttagaa agcgacttgt tctacgcagg tactcgtcca
    10801 gcggttgatg ccggactatc tgtatctcgt gtaggaggtt ctgcgcaaat caaagcaatg
    10861 aaaaaagttg ctgggacttt acgcctagac ttagcaagtt accgtgaatt agaagcattt
    10921 acacaattcg gttctgactt ggatgcagca acacaagcaa aactaaatcg tggacgccgt
    10981 acagtagaaa tcttgaaaca aaaattgcat gcaccgctag ctgttgaaaa acaagtagtg
    11041 attttgtatg ctttgacaca tggattctta gatagtatcc ctgtagacag tattttagac
    11101 ttcgaacatg aattattcga atatctggat accaatcatt cagatatctt tgaaacaatc
    11161 cgtacaacaa aagatcttcc agaagaagaa gcgttgaaca gtgcgattca ggaatataaa
    11221 gacatgttct tagctacaaa aggaagtaat tcctctacag aagataaatt gaaatccatt
    11281 caaaatgcat agtgaggtga gatgaatggg tgcttcatta aatgaaatca agacacgtat
    11341 cgcttcaacg aaaaaaacga gccaaatcac gcgggcaatg cagatggtgt ctgcttctaa
    11401 gttgacaaaa tctgaagctt cttcacagaa gtttcaggtt tatgcaacaa aagtaagaga
    11461 aatcgttacg catttgactg ctactcagtt aaatgatatt gcctcagaaa atcctagagg
    11521 ggatatcaat tacaacagca tgctgctcag ccgtcctgtg aagaaaactg gttatatcgt
    11581 catcactgca gatggcggtt tagtcggtgg atataacagc tcgatattga aacaaacgat
    11641 gtctatttta gaagaagacc accaatcacc cgatgattac gtgatgatcg caattggcgg
    11701 aactggtgca gacttcttta aagcacgcgg tatcaatcta gcatatgagc tgcgcaatct
    11761 atctgatcag ccaagctttg atgaagtccg caaaattgta aatatggcaa caactatgtt
    11821 ccaaaacgaa gtattcgatg aattatacgt atgttataac catcatatca attcgctaac
    11881 tagtcagttt cgtgtagaaa aaatgctgcc aatctcagac ttagatccag aagaagcaac
    11941 gactttcgaa caagaatata tttttgaacc ttcaaaagaa gaaatcttgt ctcagttatt
    12001 gcctcagtat gctgaaagtt tgatctacgg tgcgatcgtt gacgccaaaa cagcagaaca
    12061 tgctgctggt atgacagcaa tgaaaacagc aactgataat gctgctaata tcattgatga
    12121 gctgacggtt tcttataacc gtgcaagaca aggggctatt acccaagaaa ttaccgaaat
    12181 tgttgccgga gcttcggcac tagaatagta ttgagattgg aggaaaaaag atgagttcag
    12241 gcaagattgt tcaagtaatc ggtcccgttg tcgacgtgga attttcttta gaccaatcct
    12301 taccagatat caacaacgca ttagttgttt ataaaaatga cgaaaataaa tcaaaagttg
    12361 ttcttgaagc agcgttagaa ttaggggacg gagtgatccg gaccattgcg atggaatcaa
    12421 cagatggttt acaaagagga atggaagtca ttgatacagg caaagcaatc tctgttcctg
    12481 taggtaaaga aacattaggt cgcgtattca acgtactagg agatacgatc gatttagaaa
    12541 cacctttccc agaagatgcg gaaagaagcg aaattcataa aaaagcacca gcctttgacg
    12601 aattaagtac aagtacagaa attttggaaa cagggatcaa agttatcgat ttgcttgccc
    12661 catatttaaa aggtgggaaa gtcggactat tcggtggtgc cggtgttggt aaaaccgtac
    12721 tgatccaaga actgatccat aatatcgccc aagaacatgg tggtatttct gtatttaccg
    12781 gtgtaggtga acgtactcgt gaaggtaatg acttgtatta tgaaatgaaa gattcaggag
    12841 ttatcgaaaa aacagccatg gtgttcggac aaatgaacga accaccaggt gcacgtatgc
    12901 gtgttgcttt gactggtttg acgattgcgg aatatttccg tgatgtagaa ggtcaagatg
    12961 tactgttgtt tatcgacaac attttccgtt tcactcaagc tggatctgaa gtatcagcct
    13021 tgttaggacg gatgccttct gcggttggtt atcaaccaac attggcaaca gaaatgggtc
    13081 aattgcaaga acgtatcaca tctacgaaaa aaggttcgat cacatcgatc caagcaatct
    13141 atgtgccagc cgatgactac actgacccgg cgccggcaac agcattcgcc catttggatg
    13201 cgacaaccaa cttggaacgt aaattaacag aacaagggat ttatccagcg gtagatccat
    13261 tggcttcttc ttcaagtgct ttagcacctg aaatcgtggg agaagaacat tataaagtag
    13321 caacagaagt ccaacacgta ttgcaacgat atcgtgaatt acaagatatc attgctatct
    13381 taggtatgga tgaattatcc gatcaagaaa aagtgttagt ttctcgtgcc cgcagagtcc
    13441 aattcttctt gtcacaaaac tttaacgtgg ctgaacaatt tactggtctt ccaggttcat
    13501 atgtccctgt agaagaaaca gtgaaaggat tccgcgaaat cttggaaggc aaatacgatg
    13561 atcttcctga agaagctttc cgaagtgttg gccgaatcga agacgtcgta gaaaaagcga
    13621 aaacactagg ctactagaaa ggggtgcccc atggatcaat atttaacagt aaatgtggta
    13681 actcctgccg gtttggtcta tgatcaccat gcagctatcg tcgttgcacg tacgacagct
    13741 ggtgagcttg ggattttgcc taaacatgca ccaatcatcg ttccattgac gattgatgaa
    13801 gttcgtgtaa aacgaacaga ttcagatacc cacgtcgatt ggatcgctgt taatggcgga
    13861 atcatggaag ttcgtgataa tgttgtttca atcgttgctg atagtgcgga acgtgaacgt
    13921 gatatcgatg tatctcgtgc ggaacgtgca aaacaacgcg cagaacgcca aatcgaagaa
    13981 gcaaaagcaa aacaagatac gaacgaattg aaacgggcaa ctgttgcgtt gcaccgagca
    14041 atcaatcgta tcaaagtatc taagcactca taaaatgaga tgcggatgtt tatccgtgtt
    14101 taagaacagt aaaaagaggt tatgacagaa gtggtcagct tcaagacata agaaggaatt
    14161 tacgaaaatt gcttctcaaa tttttgtgaa atctcagctt atttttcgaa gaagctactt
    14221 ctggaacacc gtatatcaga ggttgtgaaa tcagcgtttt gtcctgagta ttagagaaac
    14281 aaatggaaaa tagttcctca tattttgccg ttttttgagc taataccgag ggtcagatga
    14341 ttgaacaccg tttatttgga ataagggact gcgacaagac ttttgtctca gtcccttttt
    14401 ttgcatattt ttagcattta tttcctaaaa aatgtaaggg gattcctata tttttaagta
    14461 tacttgtttt tccttttata cattttatgg tattatcatt ttattagtta ttataaaaca
    14521 atcgttagaa aggacgtcat tatgcaagta tacggaattg atgcggtgct tcgaatcgtg
    14581 agtcatttgg cgtttatcta cttagccttc tggtctttac gttcgttacg cattgaaact
    14641 tttttcagac caatgcataa tacgcaaatt agaatggcaa tcgtactttt ttcaattttt
    14701 ttagggtaca ctgcaagtaa ttttttctta gaggttatcg ctttatgccg aaatcttttc
    14761 gttagcctgt aaattttaaa aaaaggtact attttaaaag caaggatgtt ataatagaaa
    14821 agattttgag taagtgtatg ctcattggaa ttttttggag ggaacaacat ggaagagatc
    14881 atcgtaagag gtggcaatca attaaatgga acagtaagaa ttgaaggagc taaaaatgct
    14941 gttttgccta ttttagctgc tagtttgtta gcagaagaag gtattacgac attagacaac
    15001 gttccgattc tttctgatgt cttcactatg aatcaagtga ttcgtcactt aaacgtagat
    15061 gtagattttg atgaacaaaa gaatcaagta acaatcgacg cttcaagaca attagaaatc
    15121 gaagcgcctt atgagtatgt tagtcaaatg cgtgcttcaa tcgttgtgat gggtccgcta
    15181 cttgccagaa atggacatgc aaaagtagct atgcctggtg gatgtgcgat tggtaaacga
    15241 ccaatcgatt tgcatttaaa aggatttcaa gctttaggag caaaaattat tcaaaaaaac
    15301 ggctatatcg aagcaattgc ggatgaattg atcggtaaca cgatttattt agatttccct
    15361 agtgtaggtg ctactcaaaa tatcatgatg gctgccgtaa aagcaaaagg aacaaccatc
    15421 attgaaaacg tagctcgcga accagaaatc gttgatttag caaacatttt gaataaaatg
    15481 ggcgctcaag tctatggagc tggtactgaa acaatgcgta ttgaaggcgt ggatcattta
    15541 catgcagtca accactcaat cgtacaagat cgtatcgaag caggtacctt tatggttgct
    15601 gctgcaatga cacaaggcaa tgtgctgatt gcagatgcta tctctgaaca taaccgccca
    15661 ttgatttcta aattgatcga aatgggtgca gaaatcattg aagaagaagg cggagtccgt
    15721 gtcatcggac caaaacatat cttacctaca gatgtaaaaa cgatgcctca tcctggtttc
    15781 cctacagata tgcaagctca aatgacagct atccaattag ttgcagaagg tacaagtgtg
    15841 gtgactgaaa cagtgtttga aaatcgtttc caacatttag aagagatgcg ccgtatgaac
    15901 gctcatgtca aaatcgacgg aaatgtagca atcatggatg gaaaccatga attgcaaggt
    15961 gcagaagtat atgcaacaga cttgcgggca gcagcagcat tagttttagc tggcttgaaa
    16021 gcaaatggaa tcacacgtgt gcgtaacttg aattatctag accgtggata ttataatttc
    16081 catatcaaac tacagcaatt aggtgcagac gtcgaacgtg tagacatgga tcaaacttct
    16141 gctgaaaaaa cagcacaaac gattgcttaa ggaagtggct gaatgagtaa ccaacgatat
    16201 atcttattaa ctttgataaa gattttagtg gtgatcttat tgcttatcct tctttttgtg
    16261 gccggaacga tgatcggata tggtgtcatc ggtggcggca atccatttaa agtattccag
    16321 ccaagtttgt ggattcatat tcgagacttt tttcattgat aaataagaat cacagtggtt
    16381 ctagtcgata tgaagtgagc catgaagcag aacatgataa aagaaaacag aagggaacgt
    16441 tgacgttagt cgcgatccct ttttttagta aaagaaaaga aggaatctaa ttgatgaaga
    16501 agatattgat ttatttagtt agaggatatc agcgctttat ttcgcctgca tttccaccta
    16561 gctgccgtta ttacccaacc tgttccaatt atatgataaa agcaattcaa acccatggtg
    16621 cgctaaaagg gacggtaatg gggactgcac ggatcttacg ctgtcatcct tttgtcaaag
    16681 gcggtatcga ttatgtacca ttgacattcc gattaacgcg aaataagaaa gataaaaaag
    16741 aaccagatta taaaaaggtt gtgaaaaata cgggtagcac tgagcgataa agaaaaagag
    16801 gaaatttatt gtcaaagtaa caactccgca actacttata tgggggacaa aaaatgatcg
    16861 aagaactaaa caagaaagat ttaccatggt ccttgttatt agatgcagat cctgataaag
    16921 aaaaagttca agtatatgtt tccagaggga gtggctggat atggaaagaa aaagaaaaaa
    16981 caataggtgt actgatctat gtggcaaggg aaaaagaata cgaaatcgtc aatgtagcag
    17041 taaccccaac gtatcaagga aaggggattg gcggaaaatt acttgagaca gcttttcaga
    17101 agctttcaca gctagcttca aaccaaactc ggatcattat acgtactggc actactagta
    17161 gcgctgcgtt acacctctat caaaaaatgg gctatgtgga gattggtcga gtcaaagact
    17221 attttatcca tcattactct gagcccattt ttgaaaatgg agatcaatta agggatcaag
    17281 tgattttagc aattcactta cctaaaaata gctttaacta ataaactttc aattcgagaa
    17341 aatgctttca aatgctaaat agatacatta caatagcttt agcaaacaaa aaggagaata
    17401 tttttatggg aatattagtt tttgattttg gtggatcggc tgtcaaatat ggttgttggg
    17461 atggcaaaga gattaaaggt aagggacaat ttgcaacgcc tgaatcttgg gaagaaatga
    17521 aaacgcaatt atttcaagtt tataaaaaaa tactcgtttc ttttgaagtc gaaggaattg
    17581 gaatcagctc tcctggagta gtgaataatg agaaacaggt gattgaggga atcagtgcga
    17641 ttccttatat tcatggattt aatattttcc gagatttgga aactctgttt caactcccag
    17701 taacgattga aaatgacgcc aactgtgccg gaatggctga attttatgaa ggtgctgccc
    17761 gtgattatca agatgttgct tttgttgtgg caggaactgg gattggcggg gcgttgttca
    17821 cgaaaggaca aataaataaa ggggcacatc tttacggtgg agaatttggg ttgatgttct
    17881 tagatggcga taagacattc agtaaacttg gtacagcggt acagatggct tggcgttatt
    17941 gtgaacgcaa aggtctcgat aaaaatgctt ataccggaaa agatgtcttt gaattagcag
    18001 aaactggaga tgcaatagca aaagaagaag ttgagagttt ttatacgtat cttacaaagg
    18061 gattattcag tattcaattt tcgtttgatc cagaagtgat cgtattaggc ggcggggttt
    18121 ctgcaaaaga agggctcatt gatgagatca aaagccgaat gaagaagctg acagaacaat
    18181 tcgatctgca tgatttcgaa ccgcagattt tattatgtga gtacagaaac gatgctaatc
    18241 ttataggtgc agcagcaaac tatctcgctg tacgtcaagg agagaattaa gagctttaga
    18301 gaaggtgcag cttgcataaa agaagagaat gaaatgaata ggagattttt acctaaggaa
    18361 tgttttattt gctgcagtat gataaaatat ctgagaggtg aaaatgatgt caaaaaaaaa
    18421 taaagatatt gaagtgcgta tagaagaagt gaaaaaagat atcaaaggta agaattatga
    18481 agtcactcag ttatttatag ggaaaaaaat gattggtgag atcttagcct atgggccaaa
    18541 agaatatgaa attttctttg gtgaagaaga ttttggaaaa gaaaaaagtc ttgaaaatgc
    18601 gatagaaaca gtgattcgac actggaatct gcacgaataa aaaaatatca aaaaaacttt
    18661 caataaaggg ttgcaaaaat ctggaaaatt aggtattata actaagtcag gttgatacaa
    18721 acctttcgga ggagtagcga agtggctaaa cgcgacggac tgtaaatccg ttccttaggg
    18781 ttcagtggtt cgaatccact ctcctccatt tcattggggt atagccaagc ggtaaggcaa
    18841 gggactttga ctccctcatg cgttggttcg aatccagcta ccccagtata ccacaatcgt
    18901 aagctctcgt tagttcgaga gctctttttt tttgctttgc taccttgttc cttgaagatt
    18961 ctgctgaatt aagatagagt taagttaact acaagaattg gagggatcca catgaaatgg
    19021 aaagaaaaag tagacgaagc agctgaaaag ttatatgatt tggtcaaatc agaaaaatac
    19081 aatattgaag tgaatattcc gaaaaaaggc ggaaaagcgg tccaggtaaa atcaaaacgc
    19141 ccaacgaatc atacaaaaaa atggatggct aaaaaccgct aataataaca aaagatccat
    19201 aagtcggcat tttcatcgat tttagggtct tttgttattt tctaggttga acatatctaa
    19261 aaaattgttt ctgacaatag gagtagggag aacgatgtgt gtctaaatcc tctgttgatg
    19321 gatagaaacc tttatctttt tcagttgtga atcaggtata taccagttat aatagaagaa
    19381 acgaaaggac gggtgtacgt tatgtcgaat caattacctg tatatataca gattcatgac
    19441 caattaaaaa atgaaatcga acaaggtata tggaaagtcg gagaccgtct tccttcagaa
    19501 agagaattag cagtgaaatt caatgtcagc cgtatgacgt tgagacaagc aattcaaaac
    19561 ttggcagatg aaggaatctt agaaagaaag atcggttcgg gtacgtatgt tgcgcgagaa
    19621 aaagtacaag aaaaaatgtc tggtgcaaca agttttacag aaattatgga atcacaaaat
    19681 cgagtaccgt ctagtcggac aatctcttat ttcttgactt cgccaagctc aagtgaaatg
    19741 gaaaaactaa acttgagtaa agatgacacg atcttgcgaa tggaacgtat tcgtttcgca
    19801 gatgatgtcc ctatttgttt tgaagttgcc agtgttccac aaaagctgat cgatggctac
    19861 agcaaagcag agattactga atcgttgtat cgcacgctag aagaaaaagg cgggaaaaag
    19921 atcggtccag cgaatcaaac gattactgct gtgttggctt ctgagaaaat tgctgactat
    19981 ttagaggtaa aacgaagaga tgcagttctt cgtttgcgcc agatttctta ttttgaagac
    20041 ggtacgccgt ttgagtatgt gcgtacgcaa tacgtaggaa atcgttttga gttttatttg
    20101 gaaaaataaa aaacaggtga tccctgattc cttattagaa ataaaagaga atcagaatca
    20161 cctgttttta tatatgatca gttatcagtt gctcgtgata agaaacagaa ccctcttcaa
    20221 attggacttg tccgttcagc agctcaatga tggattcttt aaagtggtta gcactttttt
    20281 catctacagc acagatgacg ctaacttgat cagtatattg gatttctttt ataaaaaaat
    20341 gttggagttc aagataattc tgtaattttc ctagttgcgg atacgtgatc atcacagaga
    20401 tctcctgttg caaggtacct tctacgattc caatgtcaga caatgcttga gagaccgcat
    20461 gagtgtatgc tcgtattaat ccgcctgtgc cgagtttcgt tcctccaaaa tatcgagtga
    20521 ctactgcagc tacattgatc agttgatttt ttttgaggac ttccaacatc ggtacgcccg
    20581 ccgttccact tggttctcca tcatcgctgc ttcgctggat ttcatttttt tctccaatga
    20641 tgaaggcgct gcagttatgg ttcgctttcc aatgttcttt ttttatttct tgaatgaaat
    20701 tttttgcttg gtcctctgat tctattcgtc taagagaaca gataaagcgt gattttttga
    20761 tctcaatctc actttgtcca ttttctttta ttgtacgata gcgctcgatc atcttgtcac
    20821 ctcttttttt cattttcttt attcagtata aataaagttc taggttgttt caacactgta
    20881 acggttgcgc aattgctgta gtatgcgata ataaagacaa aggcaggtgg atctggggat
    20941 gcaggaacga atgaaaaagt acgatgcaat tacgcattat ttgaagaaca atggcggctc
    21001 tcaagtaacg ttgaccttta cccaatttga cgaactgctg tttcccagta acggacttcc
    21061 caaaacagca agggaaagta cagattggtg ggcaaatgat tataaacacc ctgaaaaagg
    21121 tgcctatggc tggataaatg caggctatga agtagttgtc atcaatttgg acaaagaata
    21181 tgtcgtgttc aataaattag tgaaatctag ttggctattt gattaaataa accgcgttta
    21241 gaaaacgttg acaaatttct ttctttttat ataataaagt aacagacgcg aagaaaacgt
    21301 ttgcgtagtt tgttttaaaa aggagagaaa aatatggcta aaaaaacaat catgcttgta
    21361 tgttcggctg gtatgagcac aagtttacta gtaacaaaaa tgcaaaaagc tgctgaagca
    21421 aaaggaatcg actctgatat tttcgctgtg tccgcttctg acgcagataa caatcttgca
    21481 aataaagacg tggatgtgtt gttattaggc ccacaagttc gctttatgaa agctgatttt
    21541 gagaaacgtc tggaaccaaa aggcattcct ttagatgtca tcaatatggc tgattatggt
    21601 atgatgaatg gcgaaaaagt gttagatcaa gcaatcgcgc tcataggtga ataaatagaa
    21661 cagtataact atcgaacaag cgatccaaaa aata
//
LOCUS       DANKKG010000038        21555 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000038 DANKKG010000000
VERSION     DANKKG010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.038"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            110..1534
                     /locus_tag="PW004_001199"
     CDS             110..1534
                     /locus_tag="PW004_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDL2726184.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            1995..3104
                     /gene="pdhA"
                     /locus_tag="PW004_001200"
     CDS             1995..3104
                     /gene="pdhA"
                     /locus_tag="PW004_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HDL2726185.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            3107..4084
                     /locus_tag="PW004_001201"
     CDS             3107..4084
                     /locus_tag="PW004_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HDL2726186.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            4109..5752
                     /locus_tag="PW004_001202"
     CDS             4109..5752
                     /locus_tag="PW004_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HDL2726187.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            5760..7166
                     /gene="lpdA"
                     /locus_tag="PW004_001203"
     CDS             5760..7166
                     /gene="lpdA"
                     /locus_tag="PW004_001203"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDL2726188.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            7328..7603
                     /locus_tag="PW004_001204"
     CDS             7328..7603
                     /locus_tag="PW004_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HDL2726189.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            7609..8376
                     /locus_tag="PW004_001205"
     CDS             7609..8376
                     /locus_tag="PW004_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HDL2726190.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            8544..10373
                     /gene="typA"
                     /locus_tag="PW004_001206"
     CDS             8544..10373
                     /gene="typA"
                     /locus_tag="PW004_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HDL2726191.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            10634..12406
                     /gene="recQ"
                     /locus_tag="PW004_001207"
     CDS             10634..12406
                     /gene="recQ"
                     /locus_tag="PW004_001207"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HDL2726192.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            12520..12822
                     /locus_tag="PW004_001208"
     CDS             12520..12822
                     /locus_tag="PW004_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HDL2726193.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            12825..13988
                     /locus_tag="PW004_001209"
     CDS             12825..13988
                     /locus_tag="PW004_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HDL2726194.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            14014..17442
                     /locus_tag="PW004_001210"
     CDS             14014..17442
                     /locus_tag="PW004_001210"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HDL2726195.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            17463..18587
                     /locus_tag="PW004_001211"
     CDS             17463..18587
                     /locus_tag="PW004_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726196.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            18577..19014
                     /locus_tag="PW004_001212"
     CDS             18577..19014
                     /locus_tag="PW004_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HDL2726197.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            18998..19336
                     /locus_tag="PW004_001213"
     CDS             18998..19336
                     /locus_tag="PW004_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HDL2726198.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            19455..20015
                     /gene="rsmD"
                     /locus_tag="PW004_001214"
     CDS             19455..20015
                     /gene="rsmD"
                     /locus_tag="PW004_001214"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HDL2726199.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            20012..20503
                     /gene="coaD"
                     /locus_tag="PW004_001215"
     CDS             20012..20503
                     /gene="coaD"
                     /locus_tag="PW004_001215"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HDL2726200.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            20493..21536
                     /locus_tag="PW004_001216"
     CDS             20493..21536
                     /locus_tag="PW004_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HDL2726201.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
BASE COUNT         7313 a         3739 c         4735 g         5768 t
ORIGIN      
        1 tgataagatt tttgtcacag tcccttatgt tttgcaaaaa aagtttttct tttttctcgt
       61 aaattcggta aaatcaaaag aaaaaataga caatagaaaa gagaagctta tgtgggaaaa
      121 agtagagaaa aaacaaggac cgatttatcg tcaaattatg gaacagatta tgagagaaat
      181 cgaaaacgga aggatcaatc ctggaagtca acttgaatca gaacgaaaac tagggcaact
      241 ttttggtgtg aatcgttcca ccgttgtcca tgcattggaa gagcttaggg agcttggggt
      301 actaacgagc aaacgtggca gcggatggtt tgttaatcag actgaatggg ggaaattcgc
      361 tgttccaaga atcgattggc ggcaaatgat ctctcctcgt tatgagcaga cggactggta
      421 tgagcaaaaa ataagagaag caaagcagat ccaaaaaaca tcgtttcttg atttatacgc
      481 tagcgagatg ccagatgagc ttttgccgaa ttttgagttt ccttcttatt ctcttgaaga
      541 aatcttagcg gaagaaaaag aaatggatct tttaggctat actccgttac gtaacaaaat
      601 caaacggtat ttggaaagta aatttgaatt ggcattttcc cccaaccagc ttcttgtgac
      661 tggtggcggt cagcaagcaa tctttttgat tttacaaacg attctttcag ctggagaggg
      721 gatagctgta gagtcacctt ctttttttta ccgattacct cttttcaaag cttcaggaac
      781 gcgtttattt gggataccga tggatgaaga agggatcgac ttagaaaaac tgaaagaatc
      841 gatccataaa aataaattga aagcagtgct tatcaacccg aattttcaaa accctacagg
      901 aaaagtgatg agtcaaaaac gacgagaaaa gctagtagaa ttgtgtcgac agtatcggct
      961 tccaattatt gaagacgatg tatttacgga tctttcattt ctaaaccaag aagaagtcct
     1021 tcctatacga acgattgatc cggataatgt gttatacgtc ggttcgttat ctagggtttt
     1081 aggaaaaaca acgaaaatcg gttgggtgat aggacctgag acttttatct cgcaactagc
     1141 tcaagcgcaa gaaatgatgg agttttcgat gaatatcctt acacaaatca cagtggctaa
     1201 tgtatttaat gaagcaattg atttgaaaat gcaagatgta agaaaaaagt tgaaagaaaa
     1261 aagccgactg ataagcgctt ggggagattc acaatcgttt ttcaagcttc attcaatttt
     1321 aggtggttac tatgcctgga ttacttggga tgggaaaaaa ctagaccccg aactagcaga
     1381 aaaagtagta gatatgggtc ttggaattgc accaagtcta ctttttgggc aagatataaa
     1441 tggaatcaga atcaattaca gtaggctgga tgaaaaccaa ataccgttgt tttattcaaa
     1501 gatgaaaatt ctagaaaagt ggttaacaga ataatttcac aaagtaaata gattaaagaa
     1561 tataacagct tggatttaaa tttcaagctg tttctttttt ttcggaaaaa gaatacgata
     1621 taaagaaact aagaaaaaac cattatttgt acgtccgttc acgatgtgta aataaaataa
     1681 atgtttgcaa caaaactgta taacaccaac gtttttaaat agaaacagat ggttgtaagt
     1741 ttgattagaa aatagaaaaa atgcagatga aatataaaac gctttcctta ttgtgatttt
     1801 attcactaat gctgttaata tgcggattgg cataatttcc tttcatcgtg aaaatttgtg
     1861 cttttagctt gtatttctca taaatccatg ataatttatt aatgaagacg gttaaatagt
     1921 gatacagttt tattcactgt ttcaatcgca gaatgaagag attttcttat agatgagaga
     1981 ggaatgtgtg acaaatggca aacaaaaaag caccgatcga cttccaagca ctgctagatg
     2041 aagtcaatgc agatttccca gtatatcaag cacttgatca agatggaaac gtagttaaca
     2101 aagatttagt acctgattta tccgatgatg aactcgttga attaatgact catatggtat
     2161 ggtctcgtgt attagaccaa cgttcaacag cgctgaaccg ccaaggacgc ctaggctttt
     2221 ttgcaccaac tgctggacaa gaggcaagtc agctggctag tgcttacgca tttgataaag
     2281 aagatgttct tcttccaggt tatcgggatg tacctcaact agtaaaacat ggtttacctt
     2341 tatctcaagc ttttttatgg tctcgaggac atgctgcagg taattcttat ccggaagaat
     2401 tgaaagctct accaccacaa attatcatcg gtgcacaata tgtacaagca gccggtgttg
     2461 cattaggttt gaagaaacga aacaagaaaa atgtcgtctt cacttatact ggtgatggcg
     2521 gttcatcaca aggtgatttt tacgaagcaa tcaatttcgc tggcgcttac catgcaaatg
     2581 ccgtatttta tatccaaaac aacgggtatg cgatctctac acctcgtgaa aaacaaactg
     2641 cagcaaaaac acttgcacaa aaagctgtcg cagctgggat tcctggtatt caagtcgatg
     2701 gaatggatcc gcttgcagta tatactgtat ctaaaatggc acgggaatgg gcgatcagtg
     2761 gaaatggacc agtcctaatc gaaacactga cttatcgtta tggaccacat acactatctg
     2821 gtgacgatcc aacccgctac cgtacaaaag acacagatga cgattggcag aaaaaagatc
     2881 cattgatccg cttccgtaaa tatttgacgg aaaaaggact ttggtctgaa gaaaaagaag
     2941 aacaagtcat cgaagcaact aaagaagaaa tcaaagcagc tattgctgaa gccgacaaag
     3001 taccaaaaca aaaggtttca gatttcttga aaaacatgtt cgaagtatct ccacaatcaa
     3061 tcaaagaaca aattgcaatt tatgaagcga aggagtcgaa ataatcatgg cacaaaaaac
     3121 aatgatccaa gcaatcacag atgccttagc acttgaacta gaaaatgacg agaatgtcgt
     3181 tgtttttggt gaagatgttg ggaaaaacgg aggagttttc cgggcaactg aaggattgca
     3241 ggaaaagttc ggagaagacc gagtattcga tactccttta gctgaatctg gaatcgcagg
     3301 attgagtttc ggtcttgcgt tagaaggatt tcgtccagtt cctgaaatcc aattctttgg
     3361 ttttattttc gaagcaatgg acgaagtagt agctcaaatg gctcgtacaa gatatcgaat
     3421 gagcggcact agaaatctac ccatcacaat tcgttcgcca tttggtggcg gtgttcatac
     3481 accagaattg cattcggata atttagaagg tttgatcgct cagtctcctg gtattcgagt
     3541 agtgattcct tccaaccctt atgatgcaaa aggactattg atttcttcta ttagaagtaa
     3601 cgatccggtt gttttcctag aacacatgaa actatatcgc tcattccgag aagaagtgcc
     3661 ggatgaagct tacgaagttc ctttggataa agcagcagtt actcgtgagg gaacagatgt
     3721 atctattatc acatacggag caatggtgcg tgaagcaatc aaagcagcag acaatcttgc
     3781 aaaagaaaat atatctgttg aaatcattga tcttcgtaca gttgcgccat tagatgtaga
     3841 aacgatcatt caatccgtgg aaaaaactgg tcgcgtagtt gttgtccaag aagcacaacg
     3901 acaagcgggc gttgccgcgc aagtagtttc tgagatctca gaacgcgcta tcctttcatt
     3961 agaagcccca atcggacgag tttctgcacc tgatacagtc ttcccatttg gacaagcaga
     4021 aaatgtctgg ttgccaaatg caaaagatat cgaagacaaa gtaaaagaaa tcgcggaatt
     4081 ttaagaaaag aagggacgaa acaaagtcat ggcttatcaa tttaaattgc cggatatcgg
     4141 tgaaggaatc gcagaaggtg aaatcgtcaa atggttcgtc aaaccaggag atacgatcaa
     4201 tgaagatgat acattactag aagttcaaaa tgacaaatca gtagaagaaa tcccgtcacc
     4261 tgttacagga acggttaaaa atgtgattgt tccagaaggt accgtagcaa atgttgggga
     4321 tgtattagta gaaatcgatg caccaggaca cgaagacaat gaaggggaca gtggtgtagc
     4381 cgcagaatcc caaactccag ctaaaccggc tgctgaaccc actgttgata cagaatcagc
     4441 tggatcgtca agcgaaggag tcttccaatt taagttaccg gatatcggtg aaggaatcgc
     4501 agaaggtgaa atcgtcaaat ggttcgtcaa accaggagat acgatcaatg aagatgatac
     4561 attactagaa gttcaaaatg acaaatcagt agaagaaatt ccgtcacctg tcacagggac
     4621 agttaaaaac gtgattgttc cagaaggcac tgtagcaaat gttggggatg tattagtaga
     4681 aatcgatgct cctggacaca atagtgcacc gtcaactagt gcgccatcag ctgaagcacc
     4741 aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt gaagctgccg atccaaataa
     4801 acgtgtattg gctatgccat ctgttcgtca atttgcccgt gaaaaagatg tagatatctc
     4861 acaagtaact gcaactggaa aaggtggcag agtaacaaaa gaggacatcg aaaacttctt
     4921 ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg ccagaagcgg cagcaccgaa
     4981 agaagcagct cctgcagcag aaagcaaacc agctgcacca gcaaaaccat tcaagtccaa
     5041 cttaggagac ttggaagaac gtgtagcaat gacgccaaca cgtaaagcta tcgctaaagc
     5101 aatggtcaac agtaagcata cagctccaca cgtgacattg catgatgaag tagaagtgtc
     5161 taaactatgg gataatcgga aacgatttaa agaagttgct gctgcaaatg gtacgaaact
     5221 tactttcttg ccatatgtcg tgaaagcttt aacagcgact gttaagaaat acccagtatt
     5281 gaatgcatct atcgacgatg caaaccaaga aatcgtttat aaacattact acaatatcgg
     5341 tattgcaact gatacggatc acggactata tgtaccaaat gtgaaagatg cagaccgcaa
     5401 aggaatgttt gctattgcag acgaaatcaa tgagaaagca aaattagctc atgacggcaa
     5461 actatctgca gaagatatgc gcaatggaac aattacgatc agcaatatcg gatctgtagg
     5521 tggcggttgg tttacaccag tcatcaacta tccagaagta gctattttag gtgtaggaac
     5581 gattgcacaa caaccaatcg taaatgctga aggcgaaatc gttgtcggtc gtgtgatgaa
     5641 actgtcatta agtttcgatc accgtatcgt agatggagca actgcgcaac aagcaatgaa
     5701 taatatcaaa cgtcttttag ctgatccaga gctattaatg atggaaggat gataaacaaa
     5761 tggtagttgg agattttgct gtagaattag atacagttgt aattggtgcg ggacctggtg
     5821 gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa agtagcaatc attgaaagag
     5881 agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc ttcaaaagca ctgatagctg
     5941 caggacacca ttatcaagag tcactagatt ctgaaatgtt tggagtaact agtgaaaacg
     6001 tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa caaagttgtc catacgttga
     6061 cttctggtgt tggtttccta ttaaaaaaac ataaggttga aacaatcgaa ggagaagcgt
     6121 tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga ttcagctcaa acttattcgt
     6181 tcaataacgc aatcatcgca acaggttccc gtccaatcga gattccagga tttaaattcg
     6241 gaggacgtgt gttagattct actggcggat tagcattgaa agaagtacct aagaaatttg
     6301 tgatcatcgg tggcggtgtg attggtgccg aattgggcgg agcgtacgca aacttaggag
     6361 cagaagtaac gattcttgaa ggctcaccac aaattttacc aacttacgaa aaagatctag
     6421 taaaattagt agaagatgat ttcaagaaaa aaggcgtaac agtcgtaacg aatgcaatgg
     6481 cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa atatgcagtg gacgggaaag
     6541 aagaatccgt tactgctgat tatgtgatgg tcactgtagg tcgtcgtccg aatacagatg
     6601 atatgggctt agaacaagcg ggtgtcgaag taggagaacg aggattgatc acggtggata
     6661 aacaaggacg tacgaacgta cctaacatct atgccatcgg tgatatcgtt ccaggggcgg
     6721 cattagcaca caaagccagt tatgaagcaa aaatcgctgc tgaagcaatc tctggcaaaa
     6781 aagtagctgt agattataaa gcaatgccgg cagtagcctt cactgatcct gaattggctt
     6841 ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat cgaagcaaaa gcatataaat
     6901 tcccattttc agggaacggt cgggcattat cgcttggtaa aacagaagga ttcatccgtc
     6961 tagttacgac gatcgaagat aacgtgctta ttggcgcgca aatcggtgga gtcggcgcaa
     7021 gtgatatggt ctctgaatta gctttagcaa tcgaatcagg aatgaatgct gaagatatcg
     7081 ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt catggatgct tcggaattag
     7141 cattaggttt accaatccat atttgatttg aataatacag gcgaaacgca actgaaaaat
     7201 cagttgcgtt ttgtttttta gaattatcaa taatattatt atgtaaactt aaaaatatga
     7261 caacttcttg gtttattacc acttcctatg ctacactgaa gaggaatatg aaataggaga
     7321 gttgaagatg caagaatatc agtatccact tgatttagat tggacaacag acgaaatggt
     7381 cattgtaatg aagatgtggg aagatctgga acgtgcgaat gaaaaaggga taaacaatga
     7441 agcttttttg aagacttatc agcagtttaa aactgttgtc aaatccattg gtgaggaaaa
     7501 acgattggga cgggaatttg aaaaagcttc cggttattct ttatatcgta cagtgcagca
     7561 agcaaaaaaa aatccaaaca aaactttgag aatgaacggg tgagataaat ggaacagatg
     7621 attgatcata tcaagtcctg gcttgaagaa gctggtgaga aaatcaaatc ttctcaacaa
     7681 acattaacaa tctcgcaaaa atcaaatcga acggatctag taacaaatgt cgatcgaaac
     7741 atccagaatt ttctaatcga aaaaatccat tcctttgatc cagaggcgaa aatattggca
     7801 gaagaagaag ggtgtaatca attatcggat ctcgatggaa gagtctttat catcgatcct
     7861 attgatggca ccttgaattt tgtgattgaa ggggaaaatt tttgtattat gctcgctgtt
     7921 tatgaatccg gcgttggaaa gcttggtttc atttatgacg tgatgagaaa tgaacttttt
     7981 tggggtggaa aaggaatagg cgtgtataaa aatgatgagc cattgcctag accagcaaat
     8041 aaatccttat cggaaggctt acttggtgtg aatagttatc ttttcggtca taataaatat
     8101 ggaatccgag aaattgcgga aagcagcatg gggacacgta tgtacggatg cgctggtctt
     8161 gaactagtgg caatcttgaa aggaaaccac attgcttata tttcaaacct tagcccttgg
     8221 gattatgcag cgggaaatgt cttattggaa gagtttggta tggtaacaag cggtcttttt
     8281 ggtactcctt tgacatttga aaaaagggaa tactatttag cagggacttc ttgtgcatat
     8341 gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa aaggtagaaa tcattcacaa
     8401 aaatgataaa aacgagcaaa agattaattt tctcgaaaaa atgaaaacat tttactatat
     8461 ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa tttgccctgt cataaagaac
     8521 caataaaaaa ggagttagtt acattgaatt atagaaacga tatccgtaac gtcgcaatta
     8581 ttgcccacgt tgaccacgga aaaacaacat tagttgatga actattaaaa caatcagata
     8641 cattagacgc acatacgcaa ttgcaagaac gtgcgatgga ctcaaatgcg ctagaaaaag
     8701 aacgtggtat cacgatttta gccaaaaaca cagctgtaga ctataaaggt attcgcgtaa
     8761 acatcatgga tactccagga cacgcggact tcggtggcga agtagaacgg atcatgaaaa
     8821 tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg aacaatgcct caaacacgtt
     8881 ttgtattgaa aaaagcactg gaacaacata tcacaccaat cgttgtcgta aacaagattg
     8941 acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt tcttgaacta ttcatcgaat
     9001 taggagcaga tgatgatcag ttagactttc ctgtgattta tgcatctgca ctaaatggta
     9061 cgtcaagctt gtcagatgat ccagcagatc aagaaccaac catggcacct atttttgata
     9121 caattattga aaaaatccct gctcctgtcg ataacagcga cgaaccatta caattccaag
     9181 tttcattact tgactacaac gattatgtag gtcgtatcgg aatcggacgt gtatttagag
     9241 gaacaatcaa agtaggagac caagtagcat taattaaatt agacggaaca gttaagaaat
     9301 tccgtgtaac gaaattattt ggtttctttg gactaaaacg tctagagatt caagaagcaa
     9361 aagctggaga cttgattgct gtctcaggta tggaagatat ctttgtcggt gaaacagtta
     9421 ctcctgtgga tcatcaagat gcgttaccta tccttcacat tgacgaacca acattgcaaa
     9481 tgactttctt agttaataat tcaccatttg ccggtcgaga aggtaaattc gtaaccgctc
     9541 gtaagattga agaacgtttg atggcagaac ttcaaacaga tgtatcctta cgagttgaac
     9601 caacaaactc tccagatgca tggacagttt ctggtcgtgg agaattgcat ttgtctatct
     9661 tgattgaaaa catgcgccgt gagggatacg aattgcaagt atctcgtcca gaagttattg
     9721 aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg cgtgcaaatc gatacaccag
     9781 aagaatatat gggtagtgtt atcgagtcct tgagcttacg taaaggtgaa atgcaagata
     9841 tggtccatac aggaaacgga caaatccgtt tgacattctt aacacctgcc cgcggtttga
     9901 ttggttattc aactgaattc ctttcaatga cacgtggtta cggaatcatg aaccatactt
     9961 ttgaccaata tctgccaatg cttcctggtc aaattggcgg acgtcaccaa ggggcgcttg
    10021 tttcgatcga tacaggaaaa gcaacgactt attctatcat gagtatcgaa gaacgcggta
    10081 cagtcttcgt agaaccaggt acagaagtat atgaaggaat gattatcggg gaaaacagcc
    10141 gtgataatga tttgactgtc aacatcacga aagcaaaaca aatgacaaac gtacggtctg
    10201 ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca attgacgttg gaagaatctt
    10261 tggaattctt gaatgatgat gaatattgcg aagtaacacc agaaagcatt cgcttgagaa
    10321 aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa aaagaaaaaa taatctgtaa
    10381 aaggatgaag agagaaacca gcttgttata aaaaatagct ggtttctttt tttgtccaac
    10441 tcttttctgt ggagaagtca tctttgtcaa tggacataaa ttcagaaaat acgccgaaag
    10501 acgtttaaaa gacttttaaa aaacttattt ctagagagtt tttgtttcat aataagataa
    10561 tgtttcacga aaggagatca acttgtcctt cgaagaaaat cgaagtaaaa tggcaagaga
    10621 aaggagcggg aagatgaacg acttattaaa acaatacttt ggctatgatg agtttagacc
    10681 tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa aatgtattag gaatcatgcc
    10741 taccggaagt gggaaatcaa tctgttacca gcttccagct ttactattag atgggcttac
    10801 tgtggttgtg tcccctttaa tctctttgat gaaggatcag gtggacgcgg cgaatcaatt
    10861 aggtataccc gccactttta tcaatagctc gttagatggt tacgaaacag ctagacgctt
    10921 ccaagaaatc gatagacagc agtaccgttt actttatatc gctcctgaac gttttatcat
    10981 gccggatttt atccaagcaa tgaagcgatg gaacgtccgt atgatcgcta ttgacgaggc
    11041 acattgtatc tctcagtggg ggcatgattt ccgcccaagt tatttacaga tggcgaacca
    11101 gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg acagctacag cgactgtgca
    11161 agtagctgct gacataaaaa gacttttaaa aattccagaa aacaaccata ttcagactgg
    11221 ttttgaacga gaaaatctac gatttcaagt aattaaagat caaaaaaaag aacaatatct
    11281 aatcgaatat ttaaagatca acaaaaatca atctggtatc atttatgcag caacgagaaa
    11341 agaagtagac cgtctctatc atttgcttaa aaaattcgat ttttccgtgg gtagatatca
    11401 tggtggtcta aatgagaatg agcgaacaga aatgcaagaa gcctttttat acgatcgctt
    11461 gcagctgatt gtagcgacaa atgcatttgg tatgggtata aataaaagca atgtccgttt
    11521 tgttatccat tatcaaattc ctggctctct agaagcatac tatcaggaag ctggtcgggc
    11581 gggaagagat ggtctttcca gtgaagccat cctgcttttc tctcctcaag atatccaagt
    11641 ccaaaagttt tttatccagc agtcccaaag agaagaaggg caaaagcaaa aagaatatga
    11701 aaaattaaaa gcgatgacag aatacgtgta tattgagtcc tgtcttcagc aatacatcct
    11761 aaactatttt ggcgagacaa gcagtccgtg caaccgttgt ggtaactgtt tagatgacag
    11821 agaaatagta gaagtaacga cagaagcaca gatggttctt tcttgcttaa aacgtatggg
    11881 tgagaactat ggtaaacaaa tgctgatgaa agtcttagca ggatcgaaag aacaaaagct
    11941 gcaagcttta ggttttggac atttatctac ctatggactg atgaaaaacc aatcgcaaaa
    12001 agaaacgatg cagttaattg aatatttgat atcgaatggc tatcttctga ccatcaatgg
    12061 tgagtatcct gttttgaaag taacagaaag agggatacaa gtattgaaag gacaagaatc
    12121 tgtttatcga aaggaaccca agaaagtcca gcagctatcg gatgaagaaa ccgatacact
    12181 atttgaagtg ctgcgggaac tgcgaacgga tctagcatca gaagcaggcg ttcccccgta
    12241 tgtcgtattt tcagattcca cattgaaaga aatgagccga atacgtccat caagcagatt
    12301 ggagatgctt cagataaaag gagttggcca aagcaagctt gataaatatg gcgaagcctt
    12361 tttatctcgg ataaaaaatg cggactctaa cgttctggat aagtagaaag aacgaatacg
    12421 taaaaatgaa taaaaacaac tggttaaatc tgtttttttt gctccttatg aaaatcaaat
    12481 atgttataat taaggaatta gaatgggagg taagcacaga tggattcttt gtcgaaaaaa
    12541 cttgcgatcc aagtattaaa tgatgaagca gaacggatca aacgattaat ccgaaatcaa
    12601 aacaacagtc tctgtatctc acaatgcaaa gccttcgaag aagtggtaga tacacagatg
    12661 tacggctttt cccggcaagt cggttttgct gtccgtatag gagtaatcgg aaagaacgag
    12721 ggccagctga tgttaagtga gctagaacga gaattgaatc agttatatag tgaggtttac
    12781 caagaaattt atgataaaaa agaagttggc aaggaggaat agacttgcct aagaaagtga
    12841 aaaaaaggca tctattagac tatagcatct tgattcccta tttaatttta tgtgtattgg
    12901 gattgatcat ggtatatagt tctacgtcgt atctattatt agaaaacgga caaaatccgt
    12961 ccgcatcagt cattaaccag agtatttttt gggtgcttag cttgattgct atcgcactac
    13021 tttataaaat gaaaacagat gttttgaaaa accagcgttt gatcatggca gccatcgcag
    13081 ttttgacgat tttgctgctg attgttgtgt tttttggtaa ggagatcaat ggggcgaaag
    13141 gttggctgca aattgcagga ttctcgatcc aacctgccga atacctaaaa atcatttcta
    13201 tctggtacct gtctctgact ttatctaaaa gacaaaacag tgtacaaaaa gattttcttg
    13261 ggactgtgaa acgtccactt gcaatggtta tcggacttac tgcattagtc gctattcttc
    13321 ccgattttgg taatgcggca gttatctttt tgattatttt ggttctgctt ttagccagcg
    13381 gagtcaatta tgtgtatact ttgattgtag gagtaggagg cttttgtcta agtactttta
    13441 cgatctggct gatcaatatc acaaacggaa agatcttacc cggacgttta cagtatatct
    13501 ataatcgatt tgctatttac caaaatcctt tctcagacga attaaataaa gggcatcaac
    13561 tagtcaacgg ctactatgcc atgttcaacg gaggactatt tggcagggga ttaggtaaca
    13621 gtatccagaa aaaaggattt ttacaagaag cacaaaccga ttttatttat gcaatcgtag
    13681 tggaagaact gggagtgatt atggggattt tgatattagc gttgctattc tttatgattg
    13741 tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt caattcgctt ttgtgcattg
    13801 ggatcggcgc aatgtttttg atccaagtat ttgtcaatct tggaggaatt actggcgtta
    13861 ttcctttgac agggatcacg ttcccatttt taagtcaagg aggctccagt ttgctgatgc
    13921 tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga tgaaaaacgc aagagcttag
    13981 gtttataata aaaaaagatg taggagtggt tcgatgaaaa aagttttagt tgctaacagg
    14041 ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat taggaattaa aactgttgga
    14101 atctatgcag aagaagatga atattccgta caccgtttca aagcggatga agcatattta
    14161 gtaggacaag gaaaaaaacc gattgatgcg tatctagata ttgaagggat catttctatc
    14221 gcgaaagaat gcggagctga cgctattcat ccgggttacg gtcttttgtc ggaaaacttg
    14281 aattttgcgc aacggtgcga agaagaaggc atcatttttg ttggacccaa actgcatcat
    14341 ttagatattt ttggagataa aatcaaagca aaagctgcag ctatcgaagc ggggattgct
    14401 tcgattccag gcacagatgg accaatcgct tctatagacg atgccttgga gtttgcgaaa
    14461 caatacggtt atccgattat gatcaaggca gctctaggcg gcggcggacg cggcatgcgt
    14521 gtagctcacg atgaaaagag tgcaagagag ggctacgaac gtgcgaaaag cgaagcaaaa
    14581 gcagcctttg gtagtgatga agtgtatgtt gaaaaataca tcgctaatcc taagcatatc
    14641 gaagtacaga ttctcgggga tacgcatgga aacgtcatcc atctttttga aagagattgt
    14701 tctgttcagc gccgtcacca aaaagtagta gaagtagcgc cttgtgtctc tatgaatgaa
    14761 cagcaacgtc aaaaaatctg ccaggcagct gtccaattga tgaaatatgt cgggtatgtc
    14821 aacgcaggta cagtagaatt tcttgttgaa ggagatgatt tttactttat cgaagtcaac
    14881 cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta cagatattga tatcgtaaca
    14941 acgcaactgt tgattgctca aggtttggat ctgcataaag aaatcggatt gcctcagcaa
    15001 gaaggaatta aattaaatgg ttcagccatt caatgccgaa tcactacaga agacccgttg
    15061 aataactttt taccagacac agggaaaatc gatacgtatc gttcacctgg tggttttggc
    15121 gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag tgacaccgta ttttgattca
    15181 ttgctagtaa aagtatgtac gcatggtgct acctttgaaa cagccattca gaaaatggaa
    15241 cgttgcttga aagaatttag aatccgtgga gtgaaaacaa acattccatt tatgctcaat
    15301 gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa caaccttcat tgatagtaca
    15361 gcaacattat ttgaatttcc tcgactacga gaccgcggaa acaaaacgat gaaatatatc
    15421 ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg gcgaaaaacc attttatgaa
    15481 gaaccacgta tgcctaaaga tctaatcaca agaactgatt acgtaacggc taagaatgta
    15541 ttagatgcga agggtgccga tgctttggtc gaatggatca aaggacagga aaatttgttg
    15601 ttgacggata cgacatttag agacgcccat caaagtctgc tagcaactcg tgttcggaca
    15661 aaggatttca agcagattgc acgtttgact ggagaaggtc ttcctgaact cttttcaagt
    15721 gagatgtggg ggggagcaac atttgatgtg gcttaccgct ttttgaatga agatccttgg
    15781 caacggttga gaaaaattcg ttctttaatg cctaatacgt tgctgcaaat gcttttcaga
    15841 ggttcaaatg ccgttgggta ttctaattat cctgacaatg tattggtgga atttgtcaaa
    15901 gaagcagcag cgcaaggaat cgatgtattt cgaatctttg atagtttgaa ctggactcca
    15961 caaatggaaa aaagtatcca agctgtacgg gatactggta aaatcgcaga ggcagctatt
    16021 tgctatactg gcgatatcaa cgatccaagt cgggcaaaat acaatgttca gtattataaa
    16081 gacatggcaa aggaactaga acaactgggt gcgcatatca ttgcaatcaa agatatggct
    16141 gggctgctta aaccgcaggc agcttatcgt ttgattagtg aattgaaaga aacgacagat
    16201 ctgccaatcc atctgcatac tcatgatacc agcgggaatg gaatcatcac ttattctgct
    16261 gcttcaaaag caggagtaga catcgttgac gtggcaatga gtgcaatgag tgggaatact
    16321 agtcagccta gcatgagcag cttgtattat gcactggtca atggtccacg gctgccagaa
    16381 atcacgattg aaaatgcaca gaaattaaac cattattggg aagatgtgcg catgtattac
    16441 aaaccgtttg aaaacggctt gaatgctcct gaaacagaag tatatatgca tgaaatgcca
    16501 ggaggacagt attcgaatct acagcaacag gcaaaagctg tcggattagg tcatcgctgg
    16561 gatgaaatca aacaaatgta tcatactgtc aacttgatgt ttggcgatat cgttaaagta
    16621 actccatcat ctaaagtagt tggagatatg gcactattta tggtccaaaa tgatcttaca
    16681 gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc ctgaatctgt tgtgactttc
    16741 ttccaaggag aactagggca acccgttggc ggatttccta aaaaattgca aaagattatt
    16801 cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt tggctgaatc agtcgacttc
    16861 aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg aaccaaaaca agacgaagta
    16921 ttaagctatt tgatgtatcc gcaagtcttt ttggactatc aaaaagcata taatcaattt
    16981 ggcgatgtta cattactgga cacaccaaca tttttccaag gtatacgttt gggtgaaaca
    17041 atcaatgtac aaatcgaacg cggaaaaacg cttatcattc gtttagatga aatcggagaa
    17101 cccgatatcg aagggaatcg cgtactgttc ttcaatttga atggacaaag acgtgaaatc
    17161 actatcaaag ataattcgat catcagtacg gtccaaaccc gattgaaagc tgaaccgaca
    17221 aaccgagaac aaattggagc aacgatgtct ggttctgttt tggatgtatt agtgaaaaaa
    17281 ggcgataacg ttaaaaaagg tgatacgttg atgattactg aagcgatgaa aatggaaacg
    17341 gccatcgaag cacgttttga cggagaagtc gcacatgtat atgtgtcttc aggagacact
    17401 atttcgtctg gtgacctttt gattgaagtg actgagaaat aataagcgaa aggtggggaa
    17461 acatgaaaag actgggtttg ttttggctga ttctgctatt agttattgtg gcattctaca
    17521 tggaaccagt cttgtttccg ccaaaacttg aagtgaagga aaactctgat caacaaatat
    17581 caaaccaaat gacaacgtgg aaataccagg agcttaaagc aactggtttt tctacttata
    17641 tcggaaaacc gatcgatcag ctggaaaagg attttggcca agcctatgat cgattaacta
    17701 gcggatttgg ttttgaaaca agatattatg aaaataaagc tagtcatctc acatttgaag
    17761 cgaacatcca agacagcaga gtcacaactg ttaaagtact gcaaacaggg cctgctgata
    17821 tcgcgccgtt ttatttaggg atgacgatgc aagatcttac gaaaatcact actatctata
    17881 ctaatttcac ttttgagtac catgatacag aagttggaat cgaactaatg gaagaggata
    17941 tgaattatcg tccgctaatt gcttttgata acgacacttt tgctatttta ttttttgatc
    18001 aaacaacaaa cggcctattt tcagttgtgt atttagataa agatagtctt ttgaaactat
    18061 tgccatatca agtgtttggt gaaggccttc cacattatca agcagataaa tcggctgatt
    18121 gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac tttattaaat atgcttagaa
    18181 gagaagacga acttcccata tacaatcaaa cacttgcctt tcaaaaccac agtcagctgc
    18241 tactacatga ttttttgaca aggcctgagg aaatattgtc agaggaacgt atggctgatt
    18301 ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt ctcactgacg aatgatgaat
    18361 tggagaagtt aatcagtaaa caaaaattag aaaatactag tgggctattt attcatccgg
    18421 cgattgacgc tactttttcg tttttatatt ggtacagcga tccttacaac catgaccgtt
    18481 tcatggaccc gggaacggat tctgtaggca ttgctttttc taaagaaaat atgttagtct
    18541 tattgcaaga agaaaacgga gaaagtagtg atagcagtga tcgttaacga agagctgttt
    18601 caactggagg atcaatgcct ccaagtcgct gaaatgataa aaaagagtaa aacgatggct
    18661 tcttatcttg aacataaaca gtcaatggaa gaatctgcaa aagtttgcca gttgaaagaa
    18721 gcgttcttgt ctaagaaaga agcatttgaa aatgtagcgg catatggtga gtatgcacct
    18781 gattatcgtg aaaaaaaacg agctgttcga aaagcaaaaa gacagcttga tatggatgag
    18841 catgttgcag ctttccgata tagtgaaaca cagctgcaaa gtctgttgga tcaaattgtc
    18901 caagtacttg ccgaagaagt ttctaatgaa atcaaagtag atgcaggtag cccgtttttt
    18961 gaaacgaaaa aacactttgg atgcggaggt aattgtcatg caattagaca atagtgaatt
    19021 tgaaataaca aaacgacgcg ggctgatcgt atgggtctac agcttaaaac aattaaagaa
    19081 tctgaaaaag ttcggcttgt tgcattatgt ctcaagaaga atgaaatatg tcgtgcttta
    19141 cttaaatgaa gagacattgg aagaaacaga agaacgaatc cgtaaattac attttgttcg
    19201 aaacgtagaa cgttcttacc gaccggatat cgaaatgaat tttgccgaaa aaataggaaa
    19261 caaaacagta ttaaaagacg aaggtttcga aattgaagag ctaaatacag aaatccgatt
    19321 agctgaacat ctttaaaaat ctcttatgag cagtttttaa ataaaaaata acttggataa
    19381 cgatcgattt ttgatcattg tccaagtatt tttttggtat actagtacca gtaccatttc
    19441 taaggagtga gtagatgcgg ataatatcag gaaattatgg cgggcgccgt ttaaaaagtt
    19501 tagcaggggc gaacaccaga ccaactacag ataaagtaaa agaatccata ttcaatatga
    19561 ttggaccata ttttgatgga gagacagtat tggatctgtt tgcaggaagc ggtggtttag
    19621 ctatcgaagc agtatctaga ggctgcagtc atgcggtatg cgtagacaaa aattaccaag
    19681 cgctaaaaat catcaaagaa aacatcgaaa ttacgaaaga accagagaaa tttaccacaa
    19741 taaaacttga tgcggacaaa gcaatcgaac ggctagcagc ggagaaacaa caattcgact
    19801 acttgtttct tgatccgcca tatgcaaaac aaaaaataat cgatcaaatc cataaaatgg
    19861 aagaactggg acttttttct aaaaccgcag ttatcgtatg cgaaacggat aaaacagtcg
    19921 aattgccaga aactgtggct ggattccatc aaataagaaa acagatttat ggcataacgg
    19981 ccgtaactat ctatagaaaa gagggaaatc aatgaaacgt gcattattcc caggaagttt
    20041 tgatcctttt acaaaaggtc acttagatac ggtggaacgt gcggctaaat tattcgatga
    20101 ggtagtaatt ggtgttttta tcaatacttc aaagaaaagt ttgtttcccc cagaagaaag
    20161 aatgacattg atcacaaaag ctgtttctca tcttcctaat gtaaaagtca tgcatcaaga
    20221 aaatcaattg acggtagaaa cagcaaaaga gattggtgca gatgcgttga tcagaggcat
    20281 acgaagtatc aaagactttg aatatgaacg agagattgct cagatgaatc atcatctata
    20341 tgatgaattg gaaactgtct tcttattagc aaaaccagaa tatagtcacg tgagttccag
    20401 tattttaaaa gaagtactgc actttggcgg agatgtgagc agctacttac cgccagtgat
    20461 caacgaagct ttagctggaa agcgtgaatc aaatgaaagc taaacaatgg ttgaaaacag
    20521 tactgattat catatttgga atgttgatca ttggatttgt agtagttccc ttgccgtatt
    20581 atgtagaatc accgggagcc acgatcgatc tgaaagaact aattacggtc aatgataagg
    20641 aagataaagg ttctgggtct ttctctctta cctctgttgg tatcaggcaa gctacaggtt
    20701 tcttagcttt aaaagctaaa atgacccctt ttgaagaatt gatcagtgaa gaagaattga
    20761 ctggtggggc gacaaatgaa gaatataatc aaatgcaaca gtattatatg gattcttccc
    20821 aaaatgcagc aatcgaacaa gcgttaaaac ttgcagacaa gccttataaa atggaattca
    20881 aaggcgtata tgtcatgagt gtagagaaaa catcgaattt ctttggcaag ctttcagttg
    20941 gagatacagt aacaaaagta gacgggaaaa gctttcaaag tacagaagca tttatggatt
    21001 atgtaaaaag ccaaaaagtt ggacaaacaa tcgaaatcag ttatttgcat aatggtgaag
    21061 aaaaaacagc ttctggtgat ctgatcgagt taccaacaga taaaaaggca ggaataggta
    21121 tcacgcttac tgaccatacc gagatcgaat cagatacaga tgttcgtatt aattctggct
    21181 ctatcggtgg gccatcagcg ggattgatgt tcactttaga aatctatgaa caagtcactg
    21241 gtaaaaactt gcgacatggt aagcaaattg caggaactgg aacgatcaac agtgaaggag
    21301 aagttggccg aatcggagga atcgataaaa aagtggcaag tgcggataaa gctggcgtag
    21361 agattttctt tgcaccagat gatacgattt cagacgatgt taagaaagaa tatcctgaaa
    21421 taaaaaccaa ctacgaagaa gcaaaagctg ccgctaaaaa attgaattcc tcaatgaaaa
    21481 tcgtaccagt aaaaacagta caagaagcgc ttgactatct agagaaaatg aaatgaaact
    21541 aaataattaa agagg
//
LOCUS       DANKKG010000039        21090 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000039 DANKKG010000000
VERSION     DANKKG010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.039"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(132..509)
                     /locus_tag="PW004_001217"
     CDS             complement(132..509)
                     /locus_tag="PW004_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726202.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            765..1493
                     /locus_tag="PW004_001218"
     CDS             765..1493
                     /locus_tag="PW004_001218"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDL2726203.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1493..1747
                     /gene="purS"
                     /locus_tag="PW004_001219"
     CDS             1493..1747
                     /gene="purS"
                     /locus_tag="PW004_001219"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HDL2726204.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1749..2420
                     /gene="purQ"
                     /locus_tag="PW004_001220"
     CDS             1749..2420
                     /gene="purQ"
                     /locus_tag="PW004_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HDL2726205.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2421..4643
                     /gene="purL"
                     /locus_tag="PW004_001221"
     CDS             2421..4643
                     /gene="purL"
                     /locus_tag="PW004_001221"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HDL2726206.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4628..6067
                     /locus_tag="PW004_001222"
     CDS             4628..6067
                     /locus_tag="PW004_001222"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDL2726207.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6090..7142
                     /locus_tag="PW004_001223"
     CDS             6090..7142
                     /locus_tag="PW004_001223"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HDL2726208.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            7139..7717
                     /gene="purN"
                     /locus_tag="PW004_001224"
     CDS             7139..7717
                     /gene="purN"
                     /locus_tag="PW004_001224"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HDL2726209.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7714..9249
                     /gene="purH"
                     /locus_tag="PW004_001225"
     CDS             7714..9249
                     /gene="purH"
                     /locus_tag="PW004_001225"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HDL2726210.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9265..10539
                     /gene="purD"
                     /locus_tag="PW004_001226"
     CDS             9265..10539
                     /gene="purD"
                     /locus_tag="PW004_001226"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HDL2726211.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10661..11575
                     /locus_tag="PW004_001227"
     CDS             10661..11575
                     /locus_tag="PW004_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL2726212.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11708..13273)
                     /locus_tag="PW004_001228"
     CDS             complement(11708..13273)
                     /locus_tag="PW004_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDL2726213.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13266..14717)
                     /locus_tag="PW004_001229"
     CDS             complement(13266..14717)
                     /locus_tag="PW004_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL2726214.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14731..15747)
                     /locus_tag="PW004_001230"
     CDS             complement(14731..15747)
                     /locus_tag="PW004_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2726215.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15967..17055
                     /locus_tag="PW004_001231"
     CDS             15967..17055
                     /locus_tag="PW004_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HDL2726216.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17109..18635
                     /locus_tag="PW004_001232"
     CDS             17109..18635
                     /locus_tag="PW004_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDL2726217.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18637..19842
                     /locus_tag="PW004_001233"
     CDS             18637..19842
                     /locus_tag="PW004_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2726218.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20099..21076
                     /gene="guaC"
                     /locus_tag="PW004_001234"
     CDS             20099..21076
                     /gene="guaC"
                     /locus_tag="PW004_001234"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HDL2726219.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
BASE COUNT         6757 a         3578 c         4792 g         5963 t
ORIGIN      
        1 caaaaaagaa gcagatccgt tagcggttct gcttctttta gtttattata ttttcttatc
       61 aataatagga acctgatttt aaaagcggct acctctttac cgtgcctctt tacatacaaa
      121 aaatagagga attaagcgta aatggcttgg ctattataac taaaatgctg gttatggaga
      181 tgttgcagca agttttcgag aaaagcgtat tccatctctt gtccattgaa taagatctgg
      241 atacgataat gatttttgaa acgcttctgt tcatgaatct gactgattga aagttcgcct
      301 ttgtgttctg tctgataaag aaaagtcctt ttacggcatt cagcacagcg cttggttgct
      361 cggcggattt ttcggaatgt ataaaaacta gtatctttat tgcataaata aacgtctttg
      421 atcgacagca aatcattata tagacaggaa cgttcttcag ctgttaattc tgataaaaaa
      481 agctgacctt gttttattgc atcttccatc ggcaagtgcc tcctcgaacc cattatccaa
      541 atttttagtt aattctaata taccatcatt aagtataaat gtaaacgctt gttgatggta
      601 taagattttc gtttctgata aaacacgaac attaaaagaa tctaatgcct taaatgtaca
      661 tatttttgtt gatttataaa aagatgattg ctaaaattta aagtgaaaaa acgaacaaaa
      721 aacgatgtat ttgcttttaa tgtaaataaa aaggagagac gtacatggag aagaaagaac
      781 ttttgtatga aggaaaagcc aaaaaaatat atcttacttc tgatgagaac ttgatttgga
      841 tagaatattt agatcaagca actgcgttga atggcgcgag gaaagaccag ataagtggaa
      901 aaggagaatt gaataatcaa atcacaagtg caattttccg ttatctgact caaagaggaa
      961 taaaaaatca ttttattgaa caattgtcta aaaccgaaca attagcaaaa aaagtacaaa
     1021 ttatccctct ggaagtggtt atccgaaata ttgcagcagg aagtttctca aaacgtctag
     1081 gtgttcccga aggtaaagaa ttcatcaaac caatcattga attctactat aaggatgacc
     1141 agttggatga tccatttatc aatgaagagc atatctattt cttacaactt gcaaagccgg
     1201 aagaaatcag tgagctgaaa gcccaagcgt tggctattaa tgaaattttg accgaattat
     1261 ttgctaaggt cggtttggtt ttagtagatt tcaagctaga atttggccgg tcgaatggac
     1321 aaattttgct tgcggatgaa gtaacaccgg atacttgccg attatgggat gaacgtacga
     1381 atgaacatat ggacaaagac gtttaccgca gagatctagg tgatttgatc ccagtctatc
     1441 aagaagtata taaccgtcta tcggaactaa ctgaagaaaa ggagcgagta tgatgtattt
     1501 cgtaaaagta tatgtaacct ataaagattc agtgttagat cctcaagggg aggcagtaaa
     1561 aaaagcagtc catcgtatgg gatatgaaac aatcgaagat atccgaatag gaaaatactt
     1621 tgagatacaa cttgctgatg cagaagaacc aatcgataca gtgattgaaa cgatttgtga
     1681 caagctgctt gcaaatgtaa atatggaaac ataccgatat gaaatcaaaa aaatggagga
     1741 agtataacat gaaatttgcg gtcatcgttt ttccaggttc aaattgtgac gcagatatgt
     1801 tatgggcagt tcgcgagatc atgggcgctg atgccgaatt tgtacggcat gatgagtcct
     1861 ctttagaagg atttgatggg gttttactgc cgggaggatt ctcctatggt gactatttga
     1921 gatgcggtgc gattgctcgg ttttctacaa tcatggaaga agtgattcgt tttgctgaag
     1981 aagggaaacc agtattcggt acatgcaatg gattccagat attgacagaa gcaggacttt
     2041 tgccaggtgt actgagacga aatgagtctt tgcattttat ttgcaaaact gtaccattga
     2101 aagtcaaccc caagacggcc tttacttcag agtatgaagc cgacgaaata atctatcttc
     2161 cagttgctca tggggaagga aattatttct gtgacgaaga aacacttcaa gaactaaaaa
     2221 aaaataatca gattgccttt acttataatg acaacatcaa cggcagcgtg gaaaacattg
     2281 cagggattac gaacaaagca gggaatgtgt taggaatgat gcctcatcca gaacgtgcag
     2341 tagaagactt gctaggatca gaagatggga aacggttctt tgcttccatt ttaaaaaact
     2401 tcggaaaggt gaccgtatag atgaaaacat tgaaagaacc gacagctgaa gagatcaaga
     2461 aaaaaagaat ctatgccgaa tggggattga ctgatgaaga atatcgaatg atcgaagaag
     2521 atatcttagg aagaatgcct aattatacgg aaactggact gttttctgtt atgtggagcg
     2581 aacattgttc ctataaaaat tctaaaccgg ttctacgcaa atttcctact agtggacctc
     2641 atgtgttgca aggccctgga gaaggcgcag gcatcgtcga catcggtgat ggactagcag
     2701 ttgtcttcaa agcagaaagt cacaatcatc cttcagccgt ggagccttat gaaggagctg
     2761 cgacaggagt tggcggaatc atcagagata ttttcagcat gggggctcgt ccaattgcgt
     2821 tgctagattc attgaggttc ggcgaattag ataatccaag aacgaaatat ttactagaag
     2881 aagtagttgc aggtatcggc ggatacggca attgtatcgg cataccgact gtaggcggcg
     2941 aaattgcatt tgatccttgt tatgaaggaa acccattagt caatgcaatg tgcgtaggtt
     3001 tgattgaaca caaacatatc cagaaagggc aagccgctgg cgtcggtaat tcgatcatgt
     3061 atgtaggtgc aaaaacaggt cgcgacggta tccacggagc aacttttgcc tcagaagaat
     3121 ttaatcaaga agaagaacag caacgttctg ctgtacaagt aggcgatcct tttatggaaa
     3181 agctgctgct tgaagcgtgt ttggaattaa tcttgcatca ttcggattct ctaataggta
     3241 tacaggatat gggagcagca ggacttgttt catctagtgc ggaaatggct tcaaaagctg
     3301 gctcaggact tatattgaca ttagatgaag tcccacaaag agaaactggg atgacacctt
     3361 atgaaatgat gttatcggaa tcacaagaac ggatgttgat ttgtgttaaa tcaggagagg
     3421 aagaaaaaat ccaagaacta tttcagaagt atgatctaga tgcagtcact atcggtaaag
     3481 taacagacga cgggcaatat cggctatatc atcaaggaga agaagtagca aatctgccag
     3541 tcgatgcatt agctgaagat gcaccagttt atcataaaga aatgaaagaa ccagctcgaa
     3601 tcgtagaatt tcagcagtta gctccttatc agccagttat cgaagaacct ggacaagtat
     3661 tgcttgatct gctgcagcaa ccgacgattg cttcaaaacg ttcaatctac gaaacttatg
     3721 attcacaagt acaaaccaat acagttgtcc gtccaggaag cgatgctgct gtgatgaggg
     3781 ttagagggac gaataaagcg ttagctatga ccacagactg caacgcgcgc tatctgtatt
     3841 tggatccaaa aatcggagga caaatagcag tggcagaagc tgctagaaat attattgcta
     3901 gcggtggtaa gcccttggcg atcaccgact gcctgaacta tggttctcct gataaaccag
     3961 aagttttttg ggaattatcg acttcagctg acggaattgc tgctgcgtgt gaaacgttag
     4021 gaactcctgt tatttcagga aatgtctctt tatataatga aacggatggt caagcaatct
     4081 atccaacacc aatgattggt atggtcggat tgattgagga tcataaacat atcactactc
     4141 aggaattcaa aaaatcaggg gatctaatct atatattagg taagacgttt gcagattttg
     4201 atggcagtga attacaaaag atgcagcttg gaagaatcga aggtgtgatc cgaaactttg
     4261 atctatccat tgaaaagaga aaccaagagt tagtattgac agccatccaa aacggattga
     4321 ttgaaagtgc tcatgactgt tcagaaggtg gcttggcaat tgcactggca gaatcagctt
     4381 tcaaacatca gctagggata tctgtacagt ttgaattgtc ctctgctcaa ttatttgcag
     4441 aaactcaatc aagatttgtg ttgacagtcg caccggaaaa taagacacga ttcgaagaaa
     4501 tgatgggaga tgctgctgtt ttagcaggta aggtgacgga tgaagcgata atcgaaattt
     4561 cagcaactga cggtcagatc aaaatagaaa ctgcagtagc aagaaagtgc tgggaggatg
     4621 caatcgtatg tcttatgaag tgaagagttt aaatgaagaa tgcgggatct tcggtgtttg
     4681 gggtcatcca gacgccgcta gtgtggcata cttcgggctc catagtcttc agcatcgagg
     4741 acaagaaggt gcaggtatcg tgtccaatga tcgaggaaag cttaaggggc atcgagattt
     4801 ggggctgctt tctgaggtat tcaaagatca aagaaaattg cagcagttaa caggagaagc
     4861 tgcaatcgga catgtacgtt atgcaacagc aggcaatgga agtgtggaca atattcagcc
     4921 ttttctattc aaatttcatg atcagtcgat cggcttagcg cataatggta acttgatcaa
     4981 tgctcgttcc cttagaaaag agttagagga aaatggggcg atcttccatt ctaactccga
     5041 tacggagata ttgatgcatt tgatccgacg aagtgaagaa acagattttt taaataaatt
     5101 aaaaagtgct ttgaatgaag tcaaaggtgg ctttgcctat ttattaatga cagaaacggc
     5161 aatgattgcc gctttggacc caaatggttt tcgtccatta gcaatcggac aaatgaaaaa
     5221 cggagcatac gtgattgcct cggaaacttg tgcattagaa gttattgggg cacgttttat
     5281 aagagatgtc cagcctggtg agatagtgat catcaatgac gaaggaatcc agattgattg
     5341 ctttacgaag gacacgcagc tttctatttg ttcgatggaa tatatctatt ttgccagacc
     5401 agattcaaac atcgcaggga tcaatgtgca tactgcacgg aaaaatatgg gacggaattt
     5461 agcgaaagaa tcgccagtaa aagcagatat ggtcgttggc gtacctaatt cttctttgtc
     5521 agccgcaagc ggttatgcgg aagctagtgg aatcccttat gaaatcggat tagtaaaaaa
     5581 tcaatacatt gcccgaacat ttatccagcc aacgcctgag ctaagagagc aaggtgttcg
     5641 aatgaaatta tctgctgtac gaggagtagt ggaaggtaaa aaggtgatta tggtcgatga
     5701 ttcaattgtt cggggtacga caagcagacg gatcgtccat cttttgaaag aagcaggggc
     5761 aaaagaagtc catgtgagga ttgcttctcc tcctttgaaa tatccatgtt tttacgggat
     5821 tgatattcaa acgcgtaaag aactcattgc ggcaaatcac acgattgatg aaatcaaaga
     5881 atgtatcggt gcagattctt taggtttttt aagtgaagag ggtctgatcg aatcgatcgg
     5941 tctggaaaaa gatgctccgt attcaggatt gtgtatggct tatttcaacg gagattatcc
     6001 gacgccgtta tatgactatg aagaaaaata tcaggaatca ctaaaagaaa aagtatcgtt
     6061 cttttaaaag cagaccaaga agggagtata tgaattcaat ggcaaatgca tacgcaaaag
     6121 ctggcgtaga tgtcgaagct ggatatgaag tagtagaacg gatcaagaag catgtcaaaa
     6181 aaacggaacg tttaggtatg atgggcgcta taggaggatt cggcggttgt tttgatttat
     6241 cggtctttca attaaaagag ccagtccttg tctcggggac cgatggcgtg gggacgaaat
     6301 tagcagtggc aatcgaagca gacaaacacg atacaatcgg gatcgactgc gtagcgatgt
     6361 gtgtcaatga tatcttagca caaggcgcag agccgttata tttcttagac tacttagcaa
     6421 cggggaaaaa tgatccgtta cgtttggaaa atgtagtaaa aggtgtagca gatggatgtg
     6481 ttcaaggaaa tatggcgctg attggcggag aaacagctga aatgcctggt atgtactcag
     6541 gtaatgatta tgatctagcc ggatttgctg tagggatagc tgaaaaatct caattattga
     6601 caggagaaac gatcaaccca ggagatgtgt tgcttggaat agcatctagc gggattcatt
     6661 caaatgggta ctctcttgtt cgcaaaattt tctttgagca gcagcagttt tctttatcat
     6721 caaaactgcc agaattgaaa aaaacacttg gcgaagaatt gcttgagcca acgaaaatct
     6781 atgtttcttc cttgcttccg ctgatcaaag aaaagaaaat aaaaggagct gcacatatca
     6841 ctggtggagg gtttgtagaa aatatcccgc gaatgttacc agaatctatg gctgcacaaa
     6901 tcaaacttgg ctcatggccg attcttccaa tattcggctg tttacaaaaa tatggtgaaa
     6961 ttcctgagat ggagatgtat gaaatcttca atatgggcat cggtatggtc ctggcagtcc
     7021 aagaatcgaa agtaaaagaa atagtacacg aaattgagca agctggcgaa aaagtttatg
     7081 aaatcgggaa agtgatcgcc aaagaaaaac atcgtgtggt gtttatagga gaagaacgat
     7141 gagaatcgct gtttttgctt ctggaaacgg aagcaacttt caagcgctag ccgattatct
     7201 atcgaaaaaa ggattggaat cttcgattga ctggttgttt tgtgatcagc ctgaagcata
     7261 tgttttgaaa cgtgcaactg cgttgagtgt accggcagat tgtttttcac ctaaagaatt
     7321 tgattcaaaa aaagagtatg aagaagcaat tttgcataaa ttgaaagaaa aaaagatcga
     7381 tttgatcgta ttagctggat atatgcgaat cattggaccg gtattgctga aaaattatga
     7441 taaaagaatc atcaatatcc atccgtctct tttacctgcc ttccctggtt tgcatggcat
     7501 acgtgatgcc tttgaagctg gagtaaaaga aacgggtgtc acaattcatt atatcgacca
     7561 aggtgtagat actggaccaa tcatccggca ggaaaaagtg agaattgaac aggaggacac
     7621 gttcgattct ttagaagaaa aaatccatag agtagagcat cgaatctatc cagaagtgat
     7681 tagtgaaatt attgaaaatg gaggctatct gaaatgacaa gagcattgat cagtgtatca
     7741 gataaaactg ggattgtagc attcgcccaa ggtttgattg aacaaggagt agaaatcatt
     7801 tcaacaggag gaacaaagca tgcgttggat aaagctggga tcaaaacgct ttcgatcgaa
     7861 gaagtgacag ggtttcctga aatgatggat ggccgtgtca aaacacttca tcctaaaatc
     7921 catggtggtc ttttaggaag acgcgatctc cctagccata tggaggcaat ggaaacggaa
     7981 aatatccaac caatcgactt tgtttgtgtc aatttatatc ctttcaaaga aacgattatg
     8041 aaaccagatg tcgaaatagc agaagctatt gaaaacattg atatcggtgg tcctagtatg
     8101 ctccgtagtg cagcaaaaaa ccatgaattt gtcacctctg ttgttgatcc atctgactat
     8161 gaagaagtac tttcagaatt acgtcagtat gggcatactt cactttcttt cagacaaaaa
     8221 ctagcggcaa aagtctttcg tcatactgcg gcatatgatg cactgatcgc tcaatactta
     8281 acggattggg tggaagaaaa agaaccggaa aaattgacac ttacgtatga acgaaaacaa
     8341 acgcttcgtt acggtgaaaa tagccatcaa gccgctgcct tttatcagtc cgctcttctg
     8401 gaaaaatatt cgatcgcagc agcaaaacaa ctccatggga aagaactttc ttataataat
     8461 atccgagatg cagatgctgc gctacggatc atgaaggaat atgacgagcc aacggtagtc
     8521 gccttgaaac acatgaatcc ttgtggtatt gggacagcat ctacgatttt agaagcgtgg
     8581 gaagcagcgt atgaagcaga tcctgtttca atctttggtg ggatcatcgt tttgaatcga
     8641 gaagtagatc tgccaacagc agaacagatg cataagctgt ttctagaaat catcattgcc
     8701 ccttcttata cacaagaagc tttggaatta ctgcaaaaaa agaaaaacat ccgattactg
     8761 acagtagact ttgcgcagga agaaaatcaa aatcgagaag aaacggtctc agtcttaggt
     8821 ggacttcttg tacaagatca agatctggca atagaaaatg aagaagagtg gaaagtcgtg
     8881 acaaaacgta agccgactcc agcagaagca aaagcattag cgtttgcttg gagagctgtc
     8941 aaacatgtga aatctaatgc gatcgtactg gcaaacgaac gtcagacagt aggaatcgga
     9001 gccgggcaga tgaatcgagt cggttcagta aaaattgcga ttgaacaagc gcaagagaaa
     9061 atggaaggtg cagttttagc tagcgatgct tattttccaa tggatgattc ggttgaatat
     9121 gcagccaaac atggtataaa agcaatcatt cagccaggag gaagcatcaa agaccaagat
     9181 tctattgata tggcgaataa atacgatcta gcaatggtct ttactgatgt tcggcatttt
     9241 agacattaga gaggagtcgg ataaatgaac attttggtga tcggtgcagg tggtcgtgaa
     9301 catgcaataa gtaaaaaact tttagccagt ccgaaggtgg atacggtgta ttgtgcgcca
     9361 ggaaatccgg ggatgacaaa agatggtatc aattgtgtcg ctatcagtga gcgagatcat
     9421 tcagcgttaa tccattttgt taagaaagag gctattgatt ggagtatcgt tggtcctgaa
     9481 gttcctttgt taaatgggat cgtcgatgat tttatggcgg aaggattgaa aatcttcggt
     9541 ccgacaaaag cagcagctct gatcgaaggt tcgaaaagtt ttgctaaaca attaatggat
     9601 acttatcaaa tcccgacagc taaatccaga agttttgaat gttacgaaaa agcagcagcg
     9661 tatatcaaag aaaatggtgc gccaatcgta gtgaaagcgg acggcttagc agctgggaaa
     9721 ggtgtagtcg tggctaccac agtgaaggaa gcgctggatg cggcagaaca aatgttgaac
     9781 cagcatcgat ttggggatag tagcaaaaaa atcgtcgtag aagagttttt ggcaggagaa
     9841 gaattctccc ttttggcgtt tgtccgtaat accaaggtgt atccgatggt catttcgcaa
     9901 gatcataagc gggcatttga taacgataaa gggccaaata caggcggaat gggtgcttat
     9961 tcaccagtac cgcaaatttc aaaaaagatg attgaaaaag ctgtggaaga agttttaaaa
    10021 ccagcggctt tgggaatgaa agaactagga cgctctttca cgggtgtttt atatgcgggt
    10081 ttgattgcta cgaaggaagg accaaaagtg atcgaattca atgcgcgttt cggtgatcct
    10141 gaaacgcaag tcgttctttc tcggttgaag agtgactttg cgcaagtaat cgatgattta
    10201 ttagagaatc gtacagtaga gttaaagtgg cagcaagaag gatacaatgt aggcgttgtg
    10261 gttgcagctg ccggttaccc cgaagaatat gagacaggaa tggttctacc agattttctg
    10321 gaagaagagc tttctgttta ctatgctgga gtttcctcaa ataacggcaa actagttgga
    10381 gcaggaggac gtctttattt agtagaagca tacggtgaga cgatcaaaga agcacagaaa
    10441 aaaatttaca ccgcgttaat ggataaaacg acagatggaa cattttatcg tacagatatt
    10501 ggagaaaaag ctgccaatag cgaactgatc gtgaagtaaa tcagctgctt ccatttataa
    10561 aaaatacaac aaacataatc ggaagcgccg attttagtga agccttttca taaagagtgt
    10621 ggtacacttt tgttgtgtat ataaataagg aggagttcag atgaaaaaaa ttcaattagg
    10681 tacttctgat cagaaagcag ctgcagtgat cttaggctgt atgcgtatca acaatgcgga
    10741 agatccagta aaagtgattg agacagctta tgaaaacggc attgattttt ttgaccatgc
    10801 agatatttat ggaaacggtg aatgtgaaga aatctttgca gatgctctag caaaaacatc
    10861 aatcaaacga gaagatcttt ttattcaaac aaaatgtgga atcgttccag gtgtgatgtt
    10921 tgatttttct aaaaaacata tcattgaatc tgtagaaaaa agtttgaccc gtctgaaaat
    10981 ggattatgtg gatgctttac ttcttcatcg tccagataca ttggtagaac cagaggaagt
    11041 agctgaagcg tttgatgaat tggaacggtc aggaaaagtt cgttattttg gcgtcagcaa
    11101 tcaaaaacca atgcaaatcg aattgctgaa aaaatatgtt cgtcaaccat tagtagctaa
    11161 tcagctgcaa tttggcttga aacatactgg aatgatcgat caagggattc atgtcaatat
    11221 gacagatgaa gcaagtatcg atcatgacgg tagtgtactt gattattccc gattgcatga
    11281 tatgacgatc caagcatggt caccatacca gtatggattt tttgaaggtg tatttatcgg
    11341 gaacgaaaaa ttccctgaat taaaccaaac attagaagaa ctagcagcta aatatcaaac
    11401 gacaccaact ggcttggctt ctgcttggat tttacgccat ccagccaata tgcaagtgat
    11461 cgccggtacg atgaacgtag ggcgaatcaa ggaaatcgct caagcttcag aaattaatat
    11521 gtctcgggaa gattggtacg aattgtaccg tgctgctgga aacattttac cttaaagatt
    11581 gtcataagca ggtcaaaagg gctataaatt aaaaagacag aataacttga aagctgtttt
    11641 cagcttcgag atattctgtc tttttttatt gcaaatggaa agagtatcat tgtacggatt
    11701 tcgtttatta gttatttttg tttttaagga aaagctgtgt ccgatattct tttggagaaa
    11761 tcccttgttt tttcttaaat aaataactga aatagttagg gtcattataa ccaatttcaa
    11821 aagcaatatc tgaaatacga tagtctgatt gctgcaataa tttttttgct aattcgattc
    11881 tttgttctgt cagataatcg atgaatgttt ggttaaacga ttgagaaaaa atcgtactga
    11941 aatgagaggg acttaatgca acttgttgag cgacccaagt taaggacata tctggatcgg
    12001 taaaattttc tttgataaaa tccaatgcat ggttcaaaat tggctgatat ttgttccttg
    12061 aaggctgtga gggcagagca gttaagaaag aaatgaacat ataaactgtc gaacggtaaa
    12121 gcgagtcgtc ggcgctgatt gctaagcgtt gactggtgga tgtgcaatga accatttctt
    12181 gcgggatttt cttttctttc aggcagacat gctggagtag cttagtcaag acaaataaac
    12241 gatataatcg tgttcgagtt ggtgtgcctt gtggtttcgt caagtgttcg atgtacttag
    12301 atacttcgtt tttcgataat gaagacaaat caagtgagag gtcaaagtaa aagacagaat
    12361 caactggaag tttcgcttct tgaatcaaat cctcatagct gataatttgt tctttaaggg
    12421 aactggcggt ttcaagtatc atctgcgtga tctcaaaaga tgcaggtaat tcgctgattc
    12481 tttctgcgac aggaccaatg gcgacagaaa aattattttt ttgtcccttt tctaattcat
    12541 ggaccaacgt ttgggctacc tgataactgg tctcgatcac attttcttca agagaatcaa
    12601 ctattaaaaa tttgatgaat cgagaggaaa cagcagagaa gatcacttgt tgattctccc
    12661 taaataaaaa cttcaaataa tcactgagat gagcgtaatc ttcaaagtct gtactgactt
    12721 ggtttgctgc taataatacg gtcatttttt tccctgctaa agagcgattg aaagattcag
    12781 caagtttcaa tgtttcactg atcgtaagtg agcctttgaa caaaccgttc aagaaatgat
    12841 tttttttcaa ttcgaaaaca agatcagaag aagtgtgcgt ttctttttgc tgcaaatcta
    12901 aagcagttac tgctttttct attgcttgtt tcaattcgct acttttaatc ggtttaagca
    12961 gataatcatc tgcctgtacc tggattgctc ctttcatata atcgaaatcg tcgaatccac
    13021 taataaatat gagacgagtc caaggaagta gtttccttgc ttcttttgaa aaagtcaacc
    13081 cgtccatgaa aggcatttgg atatcagtca gaatgatgtc gggctgtaag tcgataattg
    13141 aagacagtgc catctcacca tctccagctt cgccgacata agtgattgga taataagaac
    13201 caaggacagt tatttgttcc cgcaagttat tgcgaatgag gtattcgtct tcgatgataa
    13261 aaactttata catgtttgtt tcctccaatt gttttgggag atagagggat catcagtgtg
    13321 acagttgttc cttctcggta tttgctgtca atcgtgatag gtttttgatc atttccgtaa
    13381 taaagaagga gacgcttgtt tacgttgtaa agtccgtagc cgatactgaa atctgaatcg
    13441 atccctttgg ctaactcttt acgtatttct gccagttttt ctgcagggat gccaatcccg
    13501 ttgtctataa ctttgaattg gatcatctca tcaattttgc atgtggtaat acgaataaat
    13561 cctttgtgac ggacgtactt cgttccatgg tatacagcat tttcaatcaa tggctgcaag
    13621 atcattttta aaatagaaac aggaaaaagt gtttctggta tatcgatctt atagtcaagc
    13681 atatggccgt atcttatttt taagatgact agataatcat tgatatggct aatttctttt
    13741 tcaagaggaa tccagtcttg tcctttgctt aaagacgtac gaaaaaagcc ggaaagtgca
    13801 tgggtcatag tcagagcttc ttcaaagcag tcttgttcga tcaaagaaat aatggcatcc
    13861 aaactgttat aaataaaatg gggagtaatc tgagcttgca atacacgaac ttcactttgt
    13921 gctaaatggt attgtttcaa agcgttctcc cgtaaagact ggtttagatc ttccgccatt
    13981 ttattcatgc tagcggtaag tgtttgtaat tccagtgttt ccggcagggt taaacgaaag
    14041 ccaaggttcc ctgaagaaag ttgttctgac atctcgatca gctgattgat cggttcttgg
    14101 acctttctct ctaaaaagcg acgattcttt ttcgcataga aaaaaatggt actaagaact
    14161 ccaagttcca ctattgttaa tagaatgagc gagcggacaa gtgtcgtatt ttttttgctt
    14221 gctaagtcga tctccacacg aacaaaatct tgtaagatat ctgacagcag ttgagtgaca
    14281 gaatctacct gattgatgat ctcttcattg gtatccataa aatcttttgc caagatatta
    14341 ttgatcagct cgttttgata atcttccagt gtatcgatcg tacgtagcga tacatctaac
    14401 gtactaattt ctttaagtgt atttgtatta tttttgatct ctcgaatatc attcctaagt
    14461 tcttctagga tattttcctc attgaactca tctacggtta cttggccaga aatcaagtcc
    14521 cacatacttt ccaaaacttt ttgatcaacc ttcaaagcaa ttgcatctgc tttttggata
    14581 tttgcgatcg tctgttgata aatcaaaagc tgttgcgaat ataacagtat gctcagtagc
    14641 aaagggataa cagcaaatag aagcatggct ttgtttgctt gggcaaaaat ctgttttatg
    14701 cttaaatttt ttgtcatgat acttctccag tcaataattt ctggttggga tgttagctat
    14761 attctgctgg gaaaagacag tctcggaaat aaaaatatct ttaggtaatg tttttcctgt
    14821 ttgaatattt ccaaaataac gagtaacggt attggctaca taccagccga catcaggttt
    14881 acactcgact acacaattga cgattcctga gcgtagcttc tcgatcatat cttcttgtcc
    14941 atcaatagtt acaatgatca aatcttctcc aggtttgatt gatgtttctt ttatcgcagc
    15001 taaagcaccg tgagtcattt catcactatg gctgtataat acatcgattt cggataaatc
    15061 atttgtttct aaatatcgtt tcatctggtc ttttcctttc atccgaataa aatcaccgac
    15121 aattcggtga acaatagtca tcggaggttt atggtcgatg gttccatctt tttttagggt
    15181 tggtttacga ttgattgatt ccaaaaatcc ttcggagcgt agcttggtcg gtgtggagtt
    15241 ctctgaacca gcaagttcta ggattctgat ttcctgtttc tttgaatttg tgaagtgatt
    15301 gctgacaaat aaacccgccc gatttccttc tgctttgaaa ctggggccaa tgtgggtgac
    15361 gaaaagctcg ggatcattcg tttgaatatg tctgtcgaca agtatgacag ggatccctgc
    15421 tcgctttgct tcttttaata cagagtccca cccggattct tgcaaaggag taaaaatgat
    15481 catatccact ttataggcaa tgaacgtccg tatatcttgg atttgccgtt cctgattcat
    15541 gtagccattt cggtagagaa cttggtaatc ttctttttca agcgcagtga taattgattc
    15601 agtatgcttt ttccgccagc tactctccgt tcctgattgc gaaaaaccaa cgactagtgg
    15661 gtcaggtgct tcctttgtat taggtgtaca ccccatcatt tggtaagcga ttacaacaag
    15721 ggctaaaaaa aataaaacac gtttcacttt aaatcactcc atctggattt gttagtacta
    15781 tcttatcaga taaaagggaa aatcaaagtc aaaaggaatc aatcgaaaaa aatccaagaa
    15841 acttaaaaaa atccaagaca aaaaaagtac acctaaaaaa tttaaagtaa atcccataaa
    15901 aaaatcaatg ttaacgcttt ctttattagc tatactcggt gtatcaattg aaatggggga
    15961 taaaaaatga aaaacaaatt tagaaagatt gttgggacta tcgctatttt gaccgcaagc
    16021 attctattaa gtgcgtgtgg caattcagga tctgcagatg atagtacggg gtatgtagga
    16081 atcgctatgc cgaccaaatc agcagaaaga tggatcgctg atgggaacaa catggtctcg
    16141 gaattagaaa aactgggtta taaaacagat cttcaatatg gagaagataa agtagaaaat
    16201 caagtagcac aaatagaaaa tatgattaca aaaggggtag atacacttgt gattgcttct
    16261 atcgatggtt cggctttaac tgatgtgtta gctaaagcaa aagaagcaga tatcaaggtg
    16321 atcgcctatg atcgtttact gatgaattca gaaaatgtcg attattacgc gacttttgat
    16381 aattttggag taggtgtatc gcaagcagct tatattgaag agcacttagg attgaaagaa
    16441 ggaaaaggac cattcacgat tgaattattt ggtggctcgc ctgacgacaa caacgcacta
    16501 atcaattaca acggagtgat gtctgtttta caaccatata tggacaacgg tcagttggtc
    16561 gtgccatctg gacagacgag tttcagtcaa atcgctacgc ttcgctggga tggttctacg
    16621 gcgcaagcaa ggatggataa tctgttaagc gcaaattata ctgaccaaac attggatgct
    16681 gttttgtcgc catatgatcc aataagttta ggaatcattt cgtcattaaa aggcgtgggg
    16741 tatgggagtg aaagtaaacc tttacctgtg attactggac aagatgcgac agttgctggc
    16801 gttaaatcga tcattgccgg ggaacaaaca caaacgatct ttaaagacac acgtatctta
    16861 gcaaaaaata cgattgaaat gatcaaagca atcagtgatg gagaagaggt tccggtcaat
    16921 gatactgaaa cttatgataa tggggtgaaa actgtcccta cgtatttagc aaatacagta
    16981 tctgtcgata aagataatta tcaagcagag ctgatcgaca ccgattatta taaagaatcc
    17041 gatctgaaaa attaagagga accaaacaag gaactaaaga gatctaagtc agagagagga
    17101 taagaattat ggcggattgt attttaaaaa tggcagatat caccaaaaaa ttttctggaa
    17161 tccgagcgtt agattgcgtg aatctgatga ttgaacgtgg ggaaatccat gcattgtgcg
    17221 gagaaaacgg agcgggaaaa tcaacattga tgaatgtctt gagcggcttg tatccatatg
    17281 gctcctatga cggggaaatc ttttatgatg gaaagctgtg tcaatttaaa gatttgaaag
    17341 atagtgaaga aaaaggcatc gtcatcattc atcaagaatt agcactaagt ccttatcttt
    17401 caataaaaga gaatattttt ctcggaaatg aacaagcaga aagaggcgtg attgattggg
    17461 aactgacaga aaagaaaaca tatgaactgc tgaataaagt cggtttgtct atcgacccga
    17521 atcttctagt atcccagatt ggcgtagggc aacagcaact agttgaaata gctaaagcat
    17581 tatcaaaatc cgtccgtctg ttgattttgg atgagcctac cgctgcattg aatgaagaag
    17641 aaagcgcgaa tttgctgaga ttgatccgag agttgaaaga gcaaggtgta acctccatca
    17701 ttatttcaca taagctgaat gaaatcgtgg atatcgcaga tcgaatcacc attttacggg
    17761 atggacagac gattgagaca ctagaaaatg aaatgatcag cgaagagcat attattcgcg
    17821 gaatggtggg cagagattta gttgatcgtt atcctgaaag aaaaccggat attggagaag
    17881 tttattttaa agtagaagac tggacggtct atcaccctct tgatcacgag cgaatcataa
    17941 acgaccacat caacttgcat ttgaaaaaag gagagattgt aggaatagct ggactgatgg
    18001 gagcgggcag aactgaattc gctatgagtc tatttggaca ttcttatgga agcaagatat
    18061 ccggacgtat attaaaggaa ggggcagaga tatcagttaa gaccgttcct tcagccattg
    18121 caaacggact cgcttatgtc tcggaggacc gaaaatcttt aggactgaat ctgctgatgg
    18181 atattcggga aaacgtgacg ctgagcagtt tggataaaat aagtcaaaaa ggagtattag
    18241 ataaagaaaa agaagtaact gaggcagaaa atttccggaa aaagatgcgt ataaaaacaa
    18301 actccatcca tcaaattgtc agtagtctta gtggtgggaa tcaacaaaaa gtcgttttat
    18361 ccaagtggct gatgacacaa cccgatattc tttttttgga tgagccaacc cgtgggatag
    18421 atgttggggc aaaatatgag atctacacga ttatcgaaga aatggcagca aatggcaaag
    18481 ctgtctgtct tatctcttct gaacttcctg aaatattagg gatgtgtgat cgaatctata
    18541 cgatgaacga aggacgtatt acaggagaag tctcgcgaga agatgcagat caggaagtct
    18601 tgatgaaatt aatgacgaaa gaagaggtgg gttaagttgg aacaggcaaa aaatcttacg
    18661 gataaaaaag agaaaaaact aaatttgaaa agtagagcag ctgatctttt tggtcgctac
    18721 agcatggtga tcattttagc gggactactt attgcgtttc aattgatgac ggatggtatt
    18781 ttctggcgtc cattgaatat aactaacctt gttctgcaaa atagccatat attagtacta
    18841 gctgcaggga tgttattagt tgtgctctta ggtcatgtgg atttgtcggt agggtcagtg
    18901 atggcgtttg ttggtgcaat tgcgggcatg atgatggtca atcgccaaat gaacccgtgg
    18961 atgacagtta tcgcatgtct tgcagtaggc gggctgatcg gcgcatggca aggtttttgg
    19021 gtagcttatg cagggatacc ggcgtttatc gtgactttag caggattgct gatgtttcgt
    19081 ggactgacac aagttgttct aggaggccaa tctctagcac cttttccagc tgttttccaa
    19141 aaaatgtcta cgggttattt accagactta agcgaaggaa gcagccttca cttcttgaca
    19201 ttgattttag gaatgatcat cacgatcgtg ttagttgttg gtcaatggcg aacaagaaat
    19261 cgtcggaaaa atcatttatt tgaagtggaa tctccaacag ctttcttggt aaaggcggta
    19321 tttacagcag tcgttgtact aggaataact tatatctttg cctcttatca agggtatccg
    19381 attattttga tcattcttgg tttgatcgtt gcagcatatg ggtttttgac aaataaaaca
    19441 gtagcaggtc gacagattta tgcgacgggc ggaaatcgaa aagcggctga actatcaggg
    19501 atcaaaacaa agcgaatcac gttctgggta ttcgtcaaca tgggagtcat ggcagcttta
    19561 gctggattag ttttagctgc tcgattgaat gcggcaacac ctcaagcagg tacatcgatg
    19621 gaactggatg caatggcggc agtatatttt ggtggtgctt cgacttccgg cggcattgga
    19681 acaatcatgg gtgcgattgt cggtggcttg gtcatgggtg tattgaacaa tggcatgtcg
    19741 attttaggcg tcggggtaga ctggcagcag gcaatcaaag gattgatctt gcttttagcc
    19801 gttgtgctag acttgtataa taagaaaaaa cgaacaacgt aggagaacgc cggttattca
    19861 gaaaaaagac tgtgacaaga catttgtcac agtctttttt tcgctaaaag cgaacattca
    19921 ttgtttcgtt tgtatagact aaaaataata tatgtttttt taccattcgt ataaattctc
    19981 tttttgctct tgattttttt gcttaattga gttaaattac atagggcgct aaaaaaagcc
    20041 ttgattaaat taataatgtt cgtgttttgc tggatgtgaa catcaggagg ataatgtaat
    20101 gaaagtattt gattacgaag atattcaatt aattcctaat aaatgtatcg taaatagccg
    20161 ttctgaatgt gatacaacgg tcacattagg taaacataca ttcaaaatgc cagtagtgcc
    20221 tgccaacatg cagacgatta ttgatgaaac aatcgcagaa ttcttagcgg aaaatggtta
    20281 tttctatatc atgcatcggt ttgatgaagc agctcgaatt ccgtttatca aaaaaatgaa
    20341 aaagcgtgga ttgattacct cgatcagtgt tggcgtcaag aaagaggaat attcctttat
    20401 agagaagttg gcagaagaat cgctaaatcc tgattatatc actatcgaca tcgcgcatgg
    20461 acatgctaac tcagttattg atatgattca gcatattaaa aaatatttgc ctgaaacttt
    20521 tgtgattgcc ggtaatgttg gtacaccgga agccgtccgt gaattggaaa atgcgggcgc
    20581 agatgcaaca aaagtcggga ttggtccagg aaaagtatgt attacaaaaa tcaagacagg
    20641 ctttgggact ggaggctggc aactggcagc tcttagatgg tgtgcgaaag cagctcgcaa
    20701 gccgatcatc gctgacggtg ggatcagaac acatggcgat attgcaaaat ctgtccgttt
    20761 tggtgcgaca atggtcatga ttggttcatt gtttgccggt cacgaggaat ctcctggcga
    20821 aacaaaagtc gaaaatggaa tcgtgtataa agaatacttt ggttctgctt cagaattcca
    20881 aaaaggcgaa aaacgaaatg ttgaaggaaa gaaaatctgg atccaacaca aaggatcttt
    20941 gaaagataca ctagtggaaa tgcaacaaga ccttcaatca tctatttctt atgctggagg
    21001 aagagatctg gaagccattc gaaaagttga ctatgtcatc gtgaaaaatt cgatttttaa
    21061 tggagacaca atctaaaaaa taaataaaat
//
LOCUS       DANKKG010000040        20974 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000040 DANKKG010000000
VERSION     DANKKG010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20974
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.040"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(372..1958)
                     /locus_tag="PW004_001235"
     CDS             complement(372..1958)
                     /locus_tag="PW004_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HDL2726220.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFVVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADQIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETIEEIE"
     gene            2209..2433
                     /locus_tag="PW004_001236"
     CDS             2209..2433
                     /locus_tag="PW004_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HDL2726221.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            2575..4326
                     /locus_tag="PW004_001237"
     CDS             2575..4326
                     /locus_tag="PW004_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2726222.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLAPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAALLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGCSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQKLEAKIEGGEA"
     gene            4326..6110
                     /locus_tag="PW004_001238"
     CDS             4326..6110
                     /locus_tag="PW004_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2726223.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPTITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLTKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            6568..7419
                     /locus_tag="PW004_001239"
     CDS             6568..7419
                     /locus_tag="PW004_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL2726224.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELYQVIQKKYKDTFGCVEKIADFLAEIFNYQMSTDEKMYLTIHIARL
                     VHENRCK"
     gene            7516..9402
                     /locus_tag="PW004_001240"
     CDS             7516..9402
                     /locus_tag="PW004_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2726225.1"
                     /translation="MGKYQELASKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGVVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAAGGMIRGLTALLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPAFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNPGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEAGETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVTNYTDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(9443..10345)
                     /locus_tag="PW004_001241"
     CDS             complement(9443..10345)
                     /locus_tag="PW004_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL2726226.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            10575..11978
                     /locus_tag="PW004_001242"
     CDS             10575..11978
                     /locus_tag="PW004_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDL2726227.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMNELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            11975..13411
                     /locus_tag="PW004_001243"
     CDS             11975..13411
                     /locus_tag="PW004_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDL2726228.1"
                     /translation="MMGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFP
                     VITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGD
                     EKEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENL
                     CRVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASA
                     IATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPA
                     YALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFAS
                     VKNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDY
                     RIDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGN
                     GTLARSKKKSFNWYKKVIDSNGENLSND"
     gene            13556..14482
                     /gene="trxB"
                     /locus_tag="PW004_001244"
     CDS             13556..14482
                     /gene="trxB"
                     /locus_tag="PW004_001244"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDL2726229.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            14695..16422
                     /locus_tag="PW004_001245"
     CDS             14695..16422
                     /locus_tag="PW004_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HDL2726230.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     TIIQKMTEE"
     gene            16438..16788
                     /locus_tag="PW004_001246"
     CDS             16438..16788
                     /locus_tag="PW004_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDL2726231.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAIIQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(16872..17732)
                     /locus_tag="PW004_001247"
     CDS             complement(16872..17732)
                     /locus_tag="PW004_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HDL2726232.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPSVEFNLFRRT
                     FVTDEKTLMQALQDALEEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            17824..18252
                     /locus_tag="PW004_001248"
     CDS             17824..18252
                     /locus_tag="PW004_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDL2726233.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            18414..18590
                     /locus_tag="PW004_001249"
     CDS             18414..18590
                     /locus_tag="PW004_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HDL2726234.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            18617..19063
                     /locus_tag="PW004_001250"
     CDS             18617..19063
                     /locus_tag="PW004_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HDL2726235.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(19219..20598)
                     /gene="brnQ"
                     /locus_tag="PW004_001251"
     CDS             complement(19219..20598)
                     /gene="brnQ"
                     /locus_tag="PW004_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDL2726236.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
BASE COUNT         6801 a         3736 c         4503 g         5934 t
ORIGIN      
        1 ttgattttgt cgctttcgtt tatgagctat ttttcttaca ccttttactt gactaatcgt
       61 aggaaaggga actgtgacaa aagtcttgtc gcagttcctt tttccgaata aacggtgctc
      121 aaaagcttgc atctgcggta ataagtccaa ccaagttaaa aacataaaaa gctgttttta
      181 cttggctgtt cttattactt gatgcaaggc agctttttca caacctctga tacacggtgt
      241 tccagaagta acttcttcgg aaataagccg aaatttcaca aaaatttgaa aagcaatttt
      301 cttaaattcc ttcttatttc ttgaagctgc ccacttctga cacaacctcc tttgtgtaaa
      361 agaatcagtt tctactcgat ttcttctatc gtttcctgca aacgctgttc atattttttg
      421 tttcctttat ataaatcacc aatcgtccat gccaacagga gaaggacaag aacaatgatt
      481 acataagcga tttgatcagc caatgctagt tgagctgagg tggcttgctc gccgaagaaa
      541 ctttcgattt gacctggtaa tcctcgtaaa ttcaagtaag tcagactaat cacagagaac
      601 cagccaagta gttttacaac aaatgaattt ttgaatcggc tccccatctc cgcttggcta
      661 ttggtcatca tcagtaaagg aatcatcgag aatggcaatg caaaagccag aaaaacttgg
      721 gattcattca tcaagttatt caaagcggta tgttcttcga tcgttccttt tgtacttgta
      781 aacaaaacac aaatcaatac aggaatcaca gaaagcaatc gtgtgactag tcttcttagc
      841 caaattggga tacgcatatg gatgaagcct tccatgatga cttgaccggt caacgtacca
      901 gtaatagtag agttctggcc agaagcaaga agtgctacag caaataaagt agacaagata
      961 ccagattttg ctacagcaat caagatacca ttgctcatag ctgatggatc agataaagct
     1021 tcatacagac caaaaaagga aggatcttgg acagaaccgg ttttgaatac agccacaccc
     1081 atgatcaata gcagtgcatt gactagaaaa gccattgaca actggatatt agaatcccaa
     1141 gtagaaaagc gtaccgcttg tgctacatct tcttcgtctt ggtgatcgat cttccgagtt
     1201 tgagaaactg ctgaatgaag atataagtta tgcggcatga ctgttgcacc aataatccct
     1261 aaagctccag tcagcggagt ttcgccgccg atgaccggtg agtctgagaa tgtgtgactg
     1321 ttagggataa atccggcaaa caaactgtgc caatcagggt tagacaagcc aacttgatag
     1381 acgaaaacga ataaaatgac gaaaatcaag cagacaacca aagcttcgat tttacgaaac
     1441 ccgacttttg tcagcaataa aaggacgaaa acatctaaca cagtgataaa tacggcaatt
     1501 accaatggaa tatcaaaaag caggtagagg gcgattgctg caccaatgac ttctgcgatg
     1561 tctgttgcca taatggctaa ttcagtcaag atccataaaa tgacacccaa tgtcttactt
     1621 gtccgtgctc ggattgcttg ggccaaatcc atttgggtca cgattcctaa ttttgctgcc
     1681 atatattgta aaagcatagc aatcagacta gacatcaaaa taactgacat caataaatat
     1741 tggaaatttt gtccaccagt gatcgaagtg gaccagttac ctggatccat ataaccaact
     1801 gctactaatg cacctggtcc agagtaagca agaagtgttt taaaaaaact ttttcccttg
     1861 ggtacatcca ctgttccatt tacttcagct aaagataaac catttgtatg ttcaataagt
     1921 cgatgcttat gaaattgttt gttggattga ttattcaaga aaaaaccacc tttcctattt
     1981 atctacgatt acattctaca cctcagaaag aaaaaattaa agaaaaagat ttggtatgcc
     2041 taattttttt tcgtggcgtg tcttgctggt taatgaaaga aattttgttc ttctctaaaa
     2101 aaaccactat tttttgcatg aaactaaaga atacgccttt cttttttcaa taaactttag
     2161 taaaataaat cattgagagc aattcggaaa tagaaaggag agatttatat ggtatcaaca
     2221 ggtattgtcg tacttattgc tattattgct ttgctaatcg gcgcagtagg tggctttttc
     2281 ctagcgcgta aatatatgaa agactattta gaaaaaaatc ctcccgttaa tgaggacatg
     2341 ttgcgatcaa tgatgatgtc aatggggcaa aaaccatctg agaagaaaat tcgccagatg
     2401 atgcagcaaa tgaagaacca agggaaaaaa taggttctga cccaaagact ttttgcgggc
     2461 tgattttttc agtccgtttt ttatttcgta atttgttaga ctattcagac tattcagcaa
     2521 aaaagacaag cacagacaat cctatgctct tgataaagac aaaggaggag taaaatgtcg
     2581 atttttagaa aattaggttg gttttttcga caggaaaaga aacattattt aattgggatt
     2641 ttctccctat tcgcagtggc cttggtacag ttagctcctc caaaagtgat tgggatcatc
     2701 attgatgaga tcgccgataa aaatttatca ataaagatca tttcctggtg gatcttatta
     2761 ttgatccttg cagctctctt acaatatctt tttcgttata tctggcggac gaatatttgg
     2821 ggaagtgcag ctcggttgga gaaagaactt cggcagcagc tattcaaaca ttttacagaa
     2881 atggacaatc tcttttacca aaaatatcgg actggagatt tgatggctca tgcaacgaat
     2941 gatttgaatg caatccaaaa tgtagcagga gctggtatct tgacctttgc agattcagtg
     3001 atcacgggcg gaacaacgat cattgcgatg attttgttta ttgattggcg cttaacactg
     3061 attgcgctga ttcctctgcc tttactagcc gttacttcac gagtgctggg gtcaaaactc
     3121 cacgatgctt ttcgtgattc gcaagaagct ttttctagta tcaatgataa gacacaagaa
     3181 agtattactg ggatcaaagt cattaaaaca tttggacaag aaaaagaaga tctcgcggat
     3241 tttgaagaaa agattgaaga tgcaattatt aaaaacaaac gagtcaattt tttagactcc
     3301 ttatttgatc cattcattac cttgattatc ggtctgtctt atgttgcaac aattatttta
     3361 ggtggaaagt atgtcttgga aggcacgatc tcaatcgggc agatggtctc atttattagc
     3421 tatatcggta tgcttgtttg gccgatgttt gcgattggtc gattgttcaa cgtattggaa
     3481 agaggaaatg cttcttatga tcgcgtcaat gaacttttgc atgaaaagac gcatatcgtg
     3541 gaacaactgg gtgcagtgca gactccggca aaaggcgagc tgaatgtagc tatttcctct
     3601 tttaactatc cgaaagaaga atcaccagca ttagaacaaa tcaaatttgc gatccaagaa
     3661 ggagaaacat taggtattgt tgggaaaact ggtgcgggaa aaacgacaat catcaaatta
     3721 ctgatgcgtg aatacgatga ttatcaagga aaaattcaat ttggcggatg ttcgatcaaa
     3781 gaatattcat tgaatgcttt attaggatca atcggctatg tgcctcaaga tcacttcttg
     3841 ttttcgatga ccattcgaga taatatccgt ttttctaatc caaaactttc agaagaagaa
     3901 gtagagcatg cggcttcttt agcatttatc aacaatgaaa tcaaggaatt accaaaaggt
     3961 tatgatacct tagttggaga acgtggagtc tctttatctg gcggacagaa acaaaggatt
     4021 tcgattgctc gagcattgat caaaaatcct gaattgctga tcttagatga tgcactttct
     4081 gcagtagatg ccaaaacaga agaagctatc ttgcataatt taaaagaaat aagaaaagag
     4141 aaaacaacca tcattgcagc tcaccgttta agcagcgtga tgcatgcaaa agaaatcatt
     4201 gttgtcgatc aagggaaaat cattgaacga ggaacccatc atgagctgct tgcattaggc
     4261 ggctggtatg aacgaatgtg ggagaaacag aaattagaag cgaagataga aggaggcgag
     4321 gcctgatgga tgaaaattat caatcggaat ggacaaaatc gattccactt aaagaacaga
     4381 agaagatcat caaacgactg atgaagttcg ccaaaccttt tagaagaacg ttcatcgttg
     4441 ctatcttatt tgcgtttgcc ctgtctgtga tcaatgtcct attgccaagg atcatccaaa
     4501 cttttatgga tgatcatctg gctaaacagt cggctacgac acaagttatt ttgttttttg
     4561 ctggtatgta cttgtttgga gtcatagtca aaagtatcat ttggtttttc caatggtatt
     4621 tgtattccat ggcttcattg aagacctatc agtatgttcg ggtaagatta tttgaaaagc
     4681 tgcatacact aggtatgcgt tattttgatc agacaccggc aggatcgact gtttctcgag
     4741 tgacaaatga cacagaaacg ctatttgaat tttggtatgt ctttttgatg gtattgactg
     4801 ggatctttgc agtcgtttct tccttcattg ccatgtttca aatcaatgga gaaattgcct
     4861 tgtggacttt ggtgttcttg ccgattttag gaatcgtcat ctggtattac cagaaattca
     4921 gttctcgtat ttaccggaat atgcgggaaa aattaagtca gctgaacacg aaattgaatg
     4981 aatcgatttc cggcatgcgt attatccagc agtttcgcca agaagctcgt ttagctaaag
     5041 aatttgaaca agtgaacaac gagtatttag acacgcgcta tgcgatgatc aaaacaaatt
     5101 cgattctgtt aagtccgatc atcaattttc tatatgcatt ggcgattgcg ttggcgttga
     5161 ctttgtttgg gatcgatgcg ctgcattctc ccgtagaagt tggactgatc tatgcattta
     5221 cgacctatgt ccagggattt tttaatccaa tgtctcaaat gatggacttt ttgagcacct
     5281 ttacagatgg aatggttgcc ggtagtcgta ttttgaaaat catggataca gaagaatatg
     5341 caccgcaaca aaatcctgga gcagatcaag cgattacttc aggtaagatc gagtttcgca
     5401 atgtaagctt ttcttatgat ggagagcaca atgtattgaa caatatctct tttgtcgcta
     5461 atccgggaga gaccgttgca ttagtggggc acaccggaag cggaaaaagc tcgatcatca
     5521 acgtattgat gcggttttac gaattttatg aaggccagat cttgattgat gatcatgaca
     5581 ttcgagaata tccaatggaa gagttacgta agaaaatggg attggtcttg caagatgcct
     5641 tcttgtttta tggagatatc gcaggaaata tccggatgat gaatccaact atcacagacg
     5701 aacagattcg ccatgcagct gagtttgttc aagcaaacaa gttcatcgat gaattaccaa
     5761 aaggatatca ctcaaaagtg atcgaacacg gtgccagcta ttctagcgga caacgacagc
     5821 tgatcacttt tgcgcgaaca atcgtgacag atcctaaaat actagtattg gatgaagcga
     5881 cagcaaatat cgatactgaa acagaaggat tgatccaaga aggattgaca aaaatgcgac
     5941 aaggacggac aacgattgcg attgcccatc ggctgtccac gattcgggat gcagaattga
     6001 ttttagtttt agacaaaggt catatagtag aacgtgggaa tcatgaaaca cttctagcgc
     6061 aaaatggttt gtatgcagat atgtacaaac tgcaaagcag tggtgtgtaa aaagtggttg
     6121 tgacagggct ccgttcagct ttaggtgata agaaaaaaat tgtaaaaaag gctttccaga
     6181 tttttgcata aatttggctt atcaccgata agctggtact ggaacaccgt atattagaag
     6241 ttgggacagt agcatagtag cattttgacc taagcgctag agtgaaaata aaacagagct
     6301 tcccagattc ttcatctgtg agcgttagtg tcgtaattac cagatttaaa aaaaatcacg
     6361 acagctagtc agaagtgccc aatagccgct aagaaagcgt tgacaaaagt gcttgctttc
     6421 gataatataa agaaaacgat acaacttgga tagtgattgc ctgatgcaag ctaaacctga
     6481 tgagtctccc atggttccat ggacggcttt ttgggttttt ctttttgttt tggatacagc
     6541 cctatatatc atggaaagga gtaaaacatg ctgattgaga aagtactgaa taacaacgtc
     6601 gtaataacca gggatgaaag aaatcaggaa atgatcgtga tgggaaaagg tttggcattc
     6661 aaaaagaaag tgggtgaccc gatttttgac tcatgcatcg acaaaaaatt tcagttgaca
     6721 aatgacggac tttcacaaca gttccaagaa ttggcacaag cgattcctct tgagtactta
     6781 gaaatttctt gtgaaatcat tgaatataca aaagaggctc tacaagtcca gttgaatgat
     6841 tcggtgtata ttgcattgac ggatcatttg tatacggcta tcgaacgtca caaaatggga
     6901 atatttgtac caaatgtatt gctgtatgat atcaaacaac tatttcctaa agagtatgcc
     6961 atcgggaaaa aaacagtgaa gaaaatctcg gataaatacc acatcaagtt atcagaagat
     7021 gaagccggat ttatcgcatt gcatttagtc aatgcacaag gcattcaaag ccaacaagac
     7081 aatacctacc tgtttactac aacaatacag gatattctaa atatcgtccg ctattactat
     7141 aagattacat ttgatgaaga ttcagtccat ttttttcgat tcgtcacaca cctacgtttt
     7201 tttctgacac gcgtcctaga tggagaaaaa tcagaagtgg gagaagtttc tgaggaattg
     7261 taccaggtta ttcaaaaaaa atataaggat acgtttggtt gcgttgagaa aatagcagac
     7321 tttttagccg agatattcaa ctaccagatg tccacggacg aaaaaatgta tttaacgatc
     7381 catattgcgc gtttagtgca tgagaatcgt tgtaaataaa taaaaaaagg aggataaatt
     7441 aacaagaacg catgaaataa gggtagtgac attcagttgg ctaaaccttc cactaataaa
     7501 ttggagggaa tagaaatggg aaaatatcaa gaattagcga gcaaaattgt agaaaatgtc
     7561 ggcgggaagg aaaatatcaa tggtttgaca cattgtatca caaggcttcg tttcaaatta
     7621 aaaaacgaag aaaaagcaaa tgatgagatc ttgaaaaata tggacggtgt tgtgacggta
     7681 atgcgagcgg gaggacagta ccaagttgtc atcgggaatc atgtgccagt cgtgtttgaa
     7741 gaggttataa aagcaggaaa ccttactttt gatgaggctg tttcaacaga aaaaatgaga
     7801 ccgtttgata tgcttattga tggaatatcg ggctgtttcc agccattcct tgctattcta
     7861 gctgctggtg ggatgattcg tggactgaca gcattacttg tcttcttagg cgcatttgac
     7921 cgcggttctg gcacatttgt tatgtttgat aatatcggtg attctgtatt ccaatttatg
     7981 cctgttatta tcggtttaac agctgcccgt aaatttaaag taaatgaatt cgtagggatg
     8041 ttgcttgggg cagcattaat gaatcctagc ttgtcgctgg aagctttgag tggtgcagca
     8101 gaagcaccac tgactaccat attttctggc actatttttg aagcacctat ttatcaaaca
     8161 gtattcggca ttccctggat tgcgcgtaac tacgcttctt ctgtcattcc cattatcttt
     8221 attgtgttgt tagctagcca aattcagaaa ccaattaaaa agttagtacc tgagatgatc
     8281 gcgaatttct tcgttccatt cttcaccgtt cttattacaa tgcccttagg ttttttactc
     8341 gttggaccag cattcacatt cgcaactgat attttaatgg ctggatttga aacactttta
     8401 gcactttcgc cagttattta tggagcaatc gtaggcttct tctggcaaat tctagtgatg
     8461 tttggtctcc attgggcaat cgtaccaatg ggattgatgc aattttctgt gaatggttgg
     8521 caaaatatca tgacacctgt tgcagtagtg tcttttggac aaactgcagc acttactgca
     8581 ctctacttta agcttagaaa cccaaaagac aaagcaatcg caataccagc cattgtttct
     8641 ggtattgtgg ggattacaga gccagctatt tatgggttta cattgccacg taaaaaaata
     8701 tttattttta cctgtgtcgg tggtgcgtta gggggcgcgt atagtggatt aatgaattta
     8761 acttcttgga atccaggtgg attaggcatc tttacaattc ccaactatat tagaccagat
     8821 ggtgatttaa cggatgtgat taatgtattg attggaatcg ccattgcgat ggtggtatcc
     8881 tttgcgctga cttttttctt ttggaaggag gaagcaggtg aaacagatga tagacagaaa
     8941 agatcaggta aagaaattgt aaaaacaccg atacaaggac aaatcgctcc actgaatgca
     9001 gcaaaggatg cagcttttgc acaaggaaca ctaggtcgag gaatcttgat ctaccctgaa
     9061 aaaggagaag tacgtgcccc ttttgacgga acagtcatga ccctgttccc gacgaaacat
     9121 gcaatcggaa tggtttctga agcaggtctg gaattgctga ttcatgtcgg attggatact
     9181 gttcagttgg aagggaaata ttttgaaagc ttggtgcagc aaggagaaag agtgaaacaa
     9241 ggcgatctgt tgctccgctt cgacaaaaag aaaatcgaag aggaaggata cagtttagaa
     9301 acacctgtga tcgtcaccaa ttacacggat tatttggata ttttggaaaa tcaaggggag
     9361 acggtcgggc cagacgatgc acttatcacc gcactgacct gaacggttag gtgaaagaat
     9421 ggactaggct taattattct ttttagtcca cttctttatt ttcaaaattg aaaggctctt
     9481 tcatgatggc tgagctgata gggcgtttat caaatttttg tcctacagaa attagatgga
     9541 ctaaattctg ttgataattt tctgcaaaaa cacaactata taacacgtct agaagataac
     9601 aaatcgatgt attgatcgta aaattaccaa ttttcgaata cagtcgttcg cgtgtcgtaa
     9661 gtcgcaaagc ggctgtactc attgaagata gcgttgaatc accgatactg gtcaaagcta
     9721 aaattggaac atgattttgt ttcaatattt ctgcggtacg aaggatcaca ttgttttcac
     9781 ccgtataaga aatcagaatc ccgcacatat cactcgtgca attgtacgct tcatagaacg
     9841 attcaccaaa agtggtgaca atcgtgacat ccattttgat gcgcttcatt ttcagcatga
     9901 aatcttgaga aatcaaagta ttcgagttac tggcgaaaat cacgattttt tttgcttgaa
     9961 gcattagttg tttggctttt tgcagttggt catgatgaat gagagaaagt gtatcttgaa
    10021 tcgtcatctg ttctaaagaa gccattttat ttgcgatcgt cataataccg tctgtggtgg
    10081 aaaaaggaag attcgcatct acgtcttgaa aattcccttc aagataggct gtttcagcta
    10141 aaaaagcatt acgaaaactg acccagccat caaaacccat tttttttgct acacggatga
    10201 gtgtggacgg gtggacataa catcgctcag caatctcttg gatggtcatt tcgccaagtg
    10261 ctggagaatc gcttaagaga aactgaatca cttcattttc tgaaggagaa aaggagattt
    10321 ctttgatttt ttcacgtaat aacatgtaga taccttcttc ttgttttttt acgtaaatta
    10381 taactttttt gaaaatgatt tgtaaacgga tttatgttat atagcttaat aaagacattt
    10441 tttgacaaga tatttacaaa aatccagtag aaaacactaa cttttgtaaa tgttactgga
    10501 aagaaacgaa tttctattgt ttgttttgtt tacaaaaaag ataataaaag tgtaaaaagt
    10561 aaaggagtga gaacatggca aaaggaatca aaatcgctac gattggtgga ggcagcagtt
    10621 acacccccga gttgatggaa ggatttatca aaagatatga ggaattaccg atacgagaaa
    10681 tctggctcgt agatgtagaa gaaggaaaag aaaagcttga gatcgtcagt cagatgtctc
    10741 agcgtatgtg ggatgcttct ccgtatgaag tcaaaatcta tgcaactctg gatcgggaag
    10801 aagcattgaa agatgcggat tttgttacga ctcagtttcg tgtcggtcaa ttagatgcac
    10861 gaatcaaaga cgagcggatc cctagttatt atgggatgat tgggcaagaa acaaatggtg
    10921 ctggcgggat attcaaagct tttcgtaccg ttccagtggt tttggcaatc gttgaagaca
    10981 tgaagcgttt atgtccaaat gcttggctga tcaatttcgc gaacccaagc gggatggtga
    11041 cagaagcagt tatcagatat ggcaaatggg ataaagtgat aggattatgc aatgtgccag
    11101 taatggcgat gatgacagaa ccaacaatgc ttggcaagaa aaaagaagag ctgatctaca
    11161 agtttgccgg tctaaatcac ttccattggc acaaagtagc agataataaa ggaaatgacc
    11221 gcacggcaga attgattgat cgtttatacg atgaagacaa tggattgccg aaaaatatct
    11281 ttgatatccc gtttttcaaa gaacaattaa aacaaatgaa catgatccct tgtggctacc
    11341 accgctatta ctaccgtgaa gaagaaatgc tggaacatgc tttagaagaa tatcaaacga
    11401 ttggtacaag agcgcagcaa gtgaaagaaa cagaagcaga gctgtttgaa ttatacaaag
    11461 atccagaatt ggatcataaa cctgaacaat tgcaaaaacg aggtggtgct tattattcag
    11521 atgctgcctg tgaaacgatt gcttcgatct atggaaataa agaaacacag atcgtggtat
    11581 ctacaaagaa tgatggagct gttcctgatc tgccggcaga ttgtgtagta gaagtcactg
    11641 cctatgttgg cgcacaaggc gcacgaaatg tagcgtttgg ttcccttcct gctgctgaaa
    11701 gaggctggtt gcaagtaatg aagaatatgg aattgctgac gatcgaagca gctgtgactg
    11761 gtgattatgg aacagcttta caagcgttta cgatcaatcc gcttatacga tcaggaaaga
    11821 cagcaaaacg gatcatgaat gagttgttta ttgcacatca gcagtattta ccgcaattca
    11881 aggaaacaat cagccggtta aaagcagaag gaatcgaagt ccaagatgaa ctggcaagag
    11941 aattatcaag caatacacat gtaatggagg aagtatgatg ggattcagaa aagatttttt
    12001 atggggcgga gctacagcag ccaatcaatg cgagggcggt tatgatgaag gcggacgtgg
    12061 gttagcgaac gtagatgttg tgccaatcgg agaagaccgt tttcctgtca ttactggaaa
    12121 aaagaaaatg tttgaatttg atgaaaatca tttttatcca gcaaaaattg cgatcgatat
    12181 gtatcatcat tataaggaag atattgcttt atttgcagaa atggggttca agacttatcg
    12241 gatgtccatt gcttggagtc gtatttttcc aatgggcgat gaaaaagagc caaatgaatc
    12301 tggtttgaaa ttttatgagg atgtctttaa agaatgtcaa aagtacggga tcgagccgct
    12361 tgtgacgatt acacattttg attgtccgat gcatttgatc aaagaatatg gtgcgtggcg
    12421 ttccagagaa ctcgttggtt tttatgaaaa tctgtgccga gtcatcttca atcgctataa
    12481 aggacttgtc aaatattggt tgacattcaa tgagatcaat atgatccttc atgcaccatt
    12541 catgggcgca gggctgtatt ttgaagaagg agaaaatgaa gaacaaatca aatatcaagc
    12601 ggcacatcat gagttggttg caagtgccat cgcaacgaag attgctcatg aagtagatcc
    12661 ggagaacaaa gtaggctgta tgctggcagc aggagcatat tatccataca cttgcaatcc
    12721 tgaagatgtt tgggcttcta gaaaatcaga tcgagaaaat tatttcttca ttgatgtcca
    12781 gtcacgagga gaatatcctg cttatgcatt gaaagaaatg gaacgaaatg gcattcagat
    12841 caaaatggaa cctggggatg ccgcattgct gaaagaacat actgttgatt ttatctcctt
    12901 ttcttattat tcttctcgag tttcgactac tgacaacgaa ctcttggaac agactgcagg
    12961 aaatatcttt gcttctgtca aaaatccata tctggaagca agtgaatggg gttggcaaat
    13021 tgatccatta ggcttgagaa taacgatgaa tgatatttat gatcgttatc aaaaaccact
    13081 atttattgta gaaaacggct taggtgcagt cgatacgccc gatgagaatg gttatgtgga
    13141 agatgactat cgaatcgact atctggcagc acatatccag gcaatgaaag atgcagtaga
    13201 attagatgga gtcgatctat taggctatac gacttgggga tgtattgatt tagtatcagc
    13261 tggtacaggt gagatgaaga aaaggtacgg attcatttat gtcgatcgtg acaacgaagg
    13321 aaatggcaca ttagcccgtt cgaagaagaa atcattcaac tggtataaaa aagtaattga
    13381 ttcaaatgga gaaaatcttt cgaatgattg atcaatgatt tataaaagtc tattgttttt
    13441 cagcggattt ttcgagtata ataaagctat tcgagaaacg caagcagaac aatagacttt
    13501 ttttcgcatt ggctgtaaag tatgcttact tgattaagta aaggagaatc tacatatgta
    13561 tgatgtaatc gttattggcg caggtcctgc tgggatgact gctgctttat atgcttcacg
    13621 ttcaaactta tccgtattga tgatcgaaca aggcgcccct ggcggccaaa tgaacaatac
    13681 ggcagaaatc gaaaactatc ctggtttcga ttcaataatg ggtcctgatc tagcttataa
    13741 gatgtatgaa ggtgtttcaa gatttgggac agaaaatgct tatggaatcg tgcaagatat
    13801 caaggatcac ggcagccaca aagaagttat ttgtgaagat aaaacttacg aaggaaaaac
    13861 agtcattatc gcaacaggct gtatccaccg taaattaggc gtacctggcg aggaagaatt
    13921 tgctggacgt ggcgtttctt actgtgcagt ttgtgatggt gcgtttttcc gtaacaaacg
    13981 tctagtagtg atcggaggag gagactcagc agtagaagaa gccatctatt tgacacaatt
    14041 tgcttctgaa gtagtcatcg tccaccgtag agatgaatta cgtgcacaaa agatcatcca
    14101 agaccgtgca tttgcgaatg aaaaaatttc ctttatgtgg gatacagtcg ttgacgaaat
    14161 cgttgggaat gaaatggtag tgacaggtgt gaaagcacgt aatgtcaaaa ccggcaatga
    14221 gtttgatttg gcagcagacg gagtattcat ctatgttgga ctggatccat taactgcacc
    14281 atttagagaa gcagggttga cgaacgaagc tggctggatt ccaacagaca acgaaatgaa
    14341 gacgaatata ccaggcgttt ttgccatcgg agatgttcgt gaaaaagatt tgcgacaaat
    14401 cactactgct gttggtgatg gtgggatcgc tggacaacaa gtgtataaat acttggaatc
    14461 tttagaagaa acaactgcat aagtttaaaa agagtggaag aacggtttaa ttgttctttt
    14521 actctttttt ttcgcgtgaa atctactgaa tccgatttat gcgaatactt gcaaccgtgc
    14581 ttgataaaag aatcctttct tgatttaagc gttttttggt cctcatactt tcatatctaa
    14641 taagtgaatg ctatacttat taagaaccat atttcagaaa gaggtgttat tttcatgtct
    14701 tgggaacaag tgtatgaaca atgggtacat gaagaaaatt tagaagaaaa cttaaaaaaa
    14761 caattaaatg atctgggtga agatcctgaa aaacttgaag atgcttttta tgcaccgttg
    14821 gaattcggta ctgcaggcat gcggggaatc ttagggcctg ggatcaatcg aatgaacatc
    14881 tatacggtaa gacaagcaac agaaggactt gcacgtttca tggatacaca aggcgaagaa
    14941 acaaaacgcc gaggtgtcgc tatcgcttat gactcacgcc atcaatcacc agaatttgcc
    15001 atggaagcag ccaaaacatt agcacatcac aatatcccat cttatgtatt cgaaagtcta
    15061 cgtccaacac cagagctttc ttttgcagtc cgtcatttga agacgtttgc gggaatcatg
    15121 gtaaccgcat cccacaatcc agcagcttac aacggatata aagtctacgg agaagatggg
    15181 ggacaaatgc ctcctgcaga cgcagatgca ttgacgaaat atgtccgtga agtcagcaat
    15241 ccgttaaaag ttgaagtatt atctgacgaa gaagctgaac atagcggatt gatcacgatc
    15301 atcggagaag aagtggatgc cgcttatcta gaagaaatca aagcagtaac gattgatcat
    15361 gaattggtgg aaacaatggg gaaagatttg aaacttgtgt acacaccact tcacggaaca
    15421 ggaaaaatgt tgggcgaacg tgcgttgaaa caagctggat tcgaacaatt cgtactagtt
    15481 ccagaacaag ctgtcgcaga cccagatttc actacagtca aatcaccaaa tccagaagaa
    15541 cactctgctt ttgagtatgc gatacgactt ggcgagaaag aaggcgctga tttgctgatt
    15601 gcaacagatc ctgatgcaga ccgtttaggt gcagccgttc gtttgccaaa tggctcctat
    15661 gaagtattga ccggaaacca aatcggagca ctgtttgctc aatatatttt ggaagcacac
    15721 caatcgcgag gcactttacc tgaaaacgca gcggtattga aatcgatcgt ttctagcgaa
    15781 ttacctacag caattgcgga acactatggg gctaccatgt tcaatgtatt gacaggattc
    15841 aaattcattg ctgaaaaaat tcaacaattt gaagaagatc attcccatac ttttatgttc
    15901 ggattcgaag aaagttacgg ttatttagtt aaacctttcg ttcgcgacaa agacgcgatc
    15961 caagcattgc tcttattagc agaagtagca gcttactata aaaaacaagg caaaacgttg
    16021 tatgatggct tgcaaacgat ttttgaaacc tatggctact acgcagaaaa aacgatttct
    16081 gtgacaatga gcggacaaga aggttctgcg aaaatctctg ctttgatgga aaaattccgt
    16141 gaagaagcac caacagaatt tgccggagca aaagtcatcc aaacagaaga cttcaaagag
    16201 ttgacgagaa cagaccgtga aggaaacgta gaaaaactaa cgacaccgcc atctgacgta
    16261 ttgaagtata cgctggaaga aaatggctgg atcgctatcc gtccttcagg aactgagccg
    16321 aaaatcaaat tttatatcgg tgtaaaagga aactcttcag aagacgcagc tgaaaaagta
    16381 gccgcttatg aaacgattat ccaaaagatg acagaagaat aaaaaaactt gagactgatg
    16441 gaaaaagtgg taaactttag aagtcagtct tttttcaagt tggaggtgaa tggattgcca
    16501 gacacgcaaa aaacaaaagt agagaaagtc agccaactat tcaaaatgtt gagtgacccc
    16561 actcgtctca aaattctgct gtatctgaaa gacggggaac aaaatgtgac agcaatcatc
    16621 caagcagtgg aaatggaaca atctgctgta tcgcatcaac tgcgtttatt aagagaaaac
    16681 catgtcgtca agtcccatcg tgagggaaaa gcgattttgt atagtcttga tgattctcac
    16741 gtactggata ttttgaacca gacactcaaa catgtggaac atcaataacg ataggtgcaa
    16801 atagagacaa actggactgt gtcaacgaat cttcttcgct gacacagtcc agttttttta
    16861 aagaaaaatt gttagtctgt ttcttcggtg gtttcagttc cataataact atggtcttct
    16921 aggtctaaag cattcaaaat aagcgaagct gtttcttcaa tagataaaga agcgacattg
    16981 attactacgc agcccaattt ttcataaaga tcattcgcaa aatcgagttc agctcgtatt
    17041 ttttcgatat cggaataagc agtatcagga tttaatccgt aagagcgcat ccgttcttta
    17101 cggatattgt ttaaaatatc tgggttattc gtcagtccca cgattttttt aggatcgatc
    17161 tcccaaagct gtttgggaat atgtgcttgt gggatcaaag gtagattggc aacttttaaa
    17221 tttttgttag ctaaaaacaa gctcaaaggc gttttggaag ttcgggatac gcctagcaaa
    17281 acgacatctg cttctagaaa cccacgagga tctttcccgt catcgtattt tacagcaaat
    17341 tccattgctt tgatccgttt gaaatagttc tcattcaata aatgaagggc gccaggttgc
    17401 cgaatgggct tgataccagt taagcgctcg atttctgata tcggcggagt catcacatca
    17461 aaactaaata attgatgttc ttcacaaaaa gaacgcgtca tctcgatcaa ttcttgattg
    17521 atcaatgtgt gcaggacaag agctttttct tctagtgcat cttgcagtgc ctgcatcaat
    17581 gttttctcat ctgtcacaaa tgttctgcgg aataaattga actcaacgct aggatattgg
    17641 gccatagtgg cttgcgctag ttttgtcgca gtttctccag cagagtcgga gatgacaaaa
    17701 aaagtaacga tttctgtaga atcggaagac atggataaaa cctcacttgc atttttttta
    17761 tgtttattat accatactct agaatgtgta cttcgtaatc attttgattt ggaggaataa
    17821 gtcatgaaaa aacagacgtt gatcgaacaa tcactagaaa cattgaaaaa taacggtttt
    17881 aaatatacta aaaaaagaga agcattgtta acttatttag tgaagcgcaa tcgctacgta
    17941 tccgctaaag aagtttttga ctatatgaat caggaattca aaggagtaag ttacgatacg
    18001 atttatcgaa atctccatga ttttgcagaa atcgatttac tggaagaaac cgatttaaat
    18061 ggagagatga agtttcgttt tcattgctgt caaggggaaa tcggccatca ccatcacttt
    18121 atctgtacgg tgtgcggaac aacgaaagaa atccatatgt gtccaatgaa ttatttcgaa
    18181 gaacagctcg gtggatgtac gatcgaaggg catagattcg agattttcgg aagatgtgaa
    18241 aattgtcaat aaggatttaa ataaaataac atttgataat tttaaaaaaa agcaagattt
    18301 ccttgacggt caaaacagca ttagatataa tatttacagg acagtcttgt tttttgagat
    18361 atatttcaat aacaattacg gttacttttt aagctcggag ggagggaatt aacatgtcaa
    18421 aaacagtcgt tcgcaaaaac gaatcacttg acgatgctct tcgtcgcttc aaacgttccg
    18481 tgtcaaaagc gggtacttta caagaatctc gtaaacgtga attctacgaa aaaccaagtg
    18541 taagacgcaa gaaaaaatct gaagcagcta gaaaacgcaa aaaattctag ttctgtgatg
    18601 aattggagtt ggtagtgtgt cactactaag tagattaaac gatgacatga aaacagcgat
    18661 gaaagcgaaa gataaggaat ctttacaagt catccgaatg atcaagtcat ctatacaaaa
    18721 cgagcagatt aaagaaggtc acgacttgac agaagaagaa gaattaacgg tgttgtctcg
    18781 cgaaatgaaa cagcgacgtg attctttgca tgagtttgaa gaagcaggac gtgacgattt
    18841 agctgaaaaa gtaaaaagtg aaattgtgat agtcgaaaaa tacatgccgg aacaactttc
    18901 cgacgaagaa attcgacaac ttgttcaaga agcaatcacg caaactggtg cttcatcaat
    18961 gaaagaattt ggcaaagtca tgggtgcgat catgcctaaa gtcaaaggaa aagcagatgg
    19021 aaaccaagta aatgccattg tgaaagaact attacaatct taattttatc atttaaactg
    19081 cggacaatca ttgtccgcag tttttttgtg ctcgtaaaaa agagatctaa aaaatagcta
    19141 gacgaaagaa taggattctt tcatctagct gtttttttcc tgtttaatat ttcttatgtt
    19201 aaattgtgtt tgatctggtt attcttctgg tagttcattt gcttcttgtt tataccctga
    19261 taggcgatag gcgtaataga agagtaagct gccagcatac ccaagtaacg ttgggaccat
    19321 ccagccaagt cccaatgcag caaaaggaac gtgatgatga taaaattcga gcatgcttgc
    19381 tacaactctt tgttgactca ctaaagcagg cgaatttgcc agcatatcca atacggcggg
    19441 aacagcagca aacaacatcg ttacttgata gacaaatggt gtcttttgga aaaattttgc
    19501 tgttaaggaa agcaaaatca acgcaagcga caatggatac aaaagcatca atactggtaa
    19561 agaccattgg atgatattgt ccaaaccagc atttgcaacg acaaaagaga cgaaagtcgt
    19621 taaccgcagc catgtcatat aactgacttt agggaacagt ttatggaaat cttgtgcaaa
    19681 agaaacaacg agtcccatag cggtagtgaa gacacctaat gtgaccatca ctcctaagaa
    19741 gctagcgccg aagttaccta aataaaaatt agtgatctga gaaagtgcag ttccgccatt
    19801 ttctgataaa gacatcttat tcaaactcat cgcaccgaga agaacaagac caaaatagat
    19861 catgacttct agcgcaacgc tcaggctacc agcttttgct gttaattctg tgactttttt
    19921 atctttgaaa ccaaggtcac ggactgcatg gacaaaagtg acacttaagg caagcaaggc
    19981 tactgcatca accgtgttat aaccttctag gacaccattt gttaaagcgt ttgtttggta
    20041 agtatctgca gcagattgat ttagatttcc cattgggtgg aataaagaaa cgacaaaaat
    20101 gattgctaat aatgcaagaa acaaggagtt tagatacttt ccgacgtatt tgaccaaccg
    20161 tgtgggatgg acagtaagga aataagcaag taaaaagaaa agagcagaga ataaaaacat
    20221 acctaatgaa ttatattttt caggcaaaaa tggtttagca gccatttcaa aagcagtagc
    20281 agctgtccgt ggtgtaccga aaaatggtcc gatcgtcaaa tgaacaagga ttaagaacaa
    20341 ggcagcatac cattttccga caggtttagc taagtcatac agaccatcac tttttgtcac
    20401 tactacagct aagatagcta gtaatgggaa aagagcgcct gagatggcaa atcctacgcc
    20461 agcactgacc cagtttgcgc cagccatttg acctaagtgg acaggaaaga ttagattacc
    20521 tgacccgaaa aacatcccga agataagtga actgaccatc aatagttgtt tccatgaata
    20581 agtttttgtt tcttccataa atttttcctc caataattat cattttgatt gacgatataa
    20641 acgttgcagc aattagtaat gccactaata tcagattcca tgaaaaccac caccttaaaa
    20701 tataaaaaaa agcccttttc cacttgggaa aagggctcta gattctagaa cggtaccacc
    20761 ttttttcgct taaaaaagct tcttcattca cggacgcttt ttgataaagt tatccgctag
    20821 cctgataatg gaggcagcca caatgatcta cgttttcgac attgactcgg aaatgatgtt
    20881 cgtctattcg atttattgtc atttctcact tacatgactc actgggaata aatcgttgaa
    20941 tagcctactg ttttcctcga cgctttgctg tatg
//
LOCUS       DANKKG010000041        20328 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000041 DANKKG010000000
VERSION     DANKKG010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.041"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(28..1764)
                     /locus_tag="PW004_001252"
     CDS             complement(28..1764)
                     /locus_tag="PW004_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HDL2726237.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(1761..2465)
                     /locus_tag="PW004_001253"
     CDS             complement(1761..2465)
                     /locus_tag="PW004_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2726238.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            2596..3099
                     /locus_tag="PW004_001254"
     CDS             2596..3099
                     /locus_tag="PW004_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HDL2726239.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            3059..3397
                     /locus_tag="PW004_001255"
     CDS             3059..3397
                     /locus_tag="PW004_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HDL2726240.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            3418..4836
                     /gene="ffh"
                     /locus_tag="PW004_001256"
     CDS             3418..4836
                     /gene="ffh"
                     /locus_tag="PW004_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HDL2726241.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            4948..5526
                     /locus_tag="PW004_001257"
     CDS             4948..5526
                     /locus_tag="PW004_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HDL2726242.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            5530..6051
                     /locus_tag="PW004_001258"
     CDS             5530..6051
                     /locus_tag="PW004_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDL2726243.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(6130..6684)
                     /locus_tag="PW004_001259"
     CDS             complement(6130..6684)
                     /locus_tag="PW004_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL2726244.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            6937..7212
                     /gene="rpsP"
                     /locus_tag="PW004_001260"
     CDS             6937..7212
                     /gene="rpsP"
                     /locus_tag="PW004_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HDL2726245.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            7224..7475
                     /locus_tag="PW004_001261"
     CDS             7224..7475
                     /locus_tag="PW004_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HDL2726246.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            7600..8391
                     /locus_tag="PW004_001262"
     CDS             7600..8391
                     /locus_tag="PW004_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726247.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            8561..9082
                     /gene="rimM"
                     /locus_tag="PW004_001263"
     CDS             8561..9082
                     /gene="rimM"
                     /locus_tag="PW004_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HDL2726248.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            9072..9833
                     /gene="trmD"
                     /locus_tag="PW004_001264"
     CDS             9072..9833
                     /gene="trmD"
                     /locus_tag="PW004_001264"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HDL2726249.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            9969..10316
                     /gene="rplS"
                     /locus_tag="PW004_001265"
     CDS             9969..10316
                     /gene="rplS"
                     /locus_tag="PW004_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HDL2726250.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            10689..11351
                     /locus_tag="PW004_001266"
     CDS             10689..11351
                     /locus_tag="PW004_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726251.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            11430..14657
                     /gene="fss3"
                     /locus_tag="PW004_001267"
     CDS             11430..14657
                     /gene="fss3"
                     /locus_tag="PW004_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HDL2726252.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITGAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            14771..15085
                     /locus_tag="PW004_001268"
     CDS             14771..15085
                     /locus_tag="PW004_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDL2726253.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            15098..15472
                     /locus_tag="PW004_001269"
     CDS             15098..15472
                     /locus_tag="PW004_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDL2726254.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            15482..15826
                     /locus_tag="PW004_001270"
     CDS             15482..15826
                     /locus_tag="PW004_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HDL2726255.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDVEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            15897..17249
                     /locus_tag="PW004_001271"
     CDS             15897..17249
                     /locus_tag="PW004_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HDL2726256.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            17309..18451
                     /locus_tag="PW004_001272"
     CDS             17309..18451
                     /locus_tag="PW004_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HDL2726257.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            18476..18730
                     /locus_tag="PW004_001273"
     CDS             18476..18730
                     /locus_tag="PW004_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726258.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            18986..20170
                     /locus_tag="PW004_001274"
     CDS             18986..20170
                     /locus_tag="PW004_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDL2726259.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            20167..20304
                     /locus_tag="PW004_001275"
     CDS             20167..20304
                     /locus_tag="PW004_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HDL2726260.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
BASE COUNT         6758 a         3617 c         4403 g         5550 t
ORIGIN      
        1 ttaaaacgca cgagcagttg ctctttttta cattttcgga atggtaacgg tgaaagtcga
       61 acctgttcca agatgactgt ctacagtaat cgtaccatta aggagttgca cgtagtcttt
      121 tacaatcgct aaccctaatc cggtcccgcc tgaatgtcgg cttcgcgcct tgtctacgcg
      181 gtaaaaacgc tcaaagatcc gttcctgatc ctcaatgtcg atacctatac caaagtcttg
      241 gacagaaaac gatagatcat ctgttgcctg atagcggata ataatctcgc tgtcgctttt
      301 tgaatattgg acagcatttt caattagatt tttgataatt ggataaagga tatctgtttt
      361 agtagtaaat ggttcgttct ttccaatcaa tcgaatcgtc aaccgtttct tttcgatgat
      421 tggctgataa ttttgaacga tttgctggaa ataaggttct gtataaattg tctggatctc
      481 gtaagaattc tcgccatctt ttgacagctg gatgatttcc cgaatcagtt tatctaaccg
      541 gatcgcatct ttttgcatga tctcaagaaa agaagtcaga gtttgcgggt catcttttgc
      601 accatccagt aaagtctcgg taaaaccaat cagtgaagtc acaggagttt ttaattcgtg
      661 agagacgttg ccaacgaaat ccttttgtag cttttctaaa cgtcgcactt tcgttaagtc
      721 atacgccacc cctagaatct gtccggtacc ttctgaatta gtaaagtagc gcaaactcaa
      781 gtctaatgtc tgccgacctt ctgtaagggt gatctcctga tggatcagcg gatgctcagg
      841 agtcacttga tgaatcagct gaatgagttt tggctcttga atgacttctg tgtaccgctg
      901 ttcaggttga tagcttacca cgtttaaatg tagctgcatc tttggattaa gcagtagtaa
      961 acgagaatca gcatcatcaa tcaggaatac gccaatcatc agttcgttca gtaacgtata
     1021 taattgctct tctgtggtag tacacgcccg atacatctta ctcatttgtt cgctcaacgc
     1081 attaatcgtc tgatacaatt cttcccattg atcgggtgtt tgcatgatta cttccgcctg
     1141 attaggattt tgcaccattt tttttagtac gggcagcacg gttttcaatg gctggttttt
     1201 ttgatagatc agataataga tcattcctgc aagaacaagg aagaaagaca gaaagaagaa
     1261 aaagacccaa cggcgaaaac tctcagttct cggtaaaaaa ccgcttgtcg gttctgcgat
     1321 acgtaaaatg gcttctaact ggccactttt tttgactggt aatgcgacat ataaaagatc
     1381 ctcattcaaa gtagtacttt tgcgcaaagc gctacctagg ttcccaccat tcaatacggc
     1441 tttgatttcg ggacgggagt ttcgctgttc attcaatgtt tcattgtttg tgtcaaataa
     1501 gatctttcca gtagcatcca ttaaagtaat ccgttcgttc gactgatgga cgaattcttc
     1561 caaagcagtt ttgttttgcg ctgcttggag gttttcgaca tctaattgcc ggatcagcag
     1621 ttgtgctttt tttgtcaggt agctttcttg ctgttccacg atctgctgtt gaaaataatg
     1681 ggaaatcaat tgccagccac caaaaaacaa actaactaaa agaataagaa agaagagacg
     1741 ttcataaatt tttttccatt tcatcgtttg ggttcctgaa acttatagcc aaatcctcta
     1801 atagtcacca agtactttgg gtgttttgga tctcgctcga ttttttctcg caaatgacta
     1861 acgtgtacat ctacgattcg gctttgtcct gcgaagtcaa aattccaaat gcggtctagc
     1921 aatgtgtctc ggtcgatcac acggtctttt cgtttcatga aataaacaag taattcaaat
     1981 tctttcggtg ttagttcaat cagatggtcg tctacggtca cttgataatt ggtcaaatca
     2041 gcagtgattt gtccaatcga tagatattca ggctcagctt catcttttga ttgcttacga
     2101 ggttcaattc gtctgaatat tgctttcata cgggctaata cttcgcgagg actaaatggt
     2161 ttggtcaaat agtcgtcagc acctatttct aggccaatga tccgatccac ttggtcatct
     2221 ttagccgtca gcatcaaaat cggtgtttca tttttttctt gacgcagccg ctgagtgatc
     2281 tccatgccat ccatcgttgg aagcatcaca tctaaaatga tgaaatcaaa tgattgttct
     2341 aatgctaatt ctaatccttc gcctccatct gtggcactgg ttaccttgta gccttctttt
     2401 tccaaattga acgtcaagag cgttacaata gaaggttcat catcaacaac tagtattttt
     2461 ttcattcaaa tctcctttaa ataggtttca ttctgtttat attttagcat agaatcagta
     2521 caaagggcta ttttacaaaa gaaataaatg gtataatagg aaggcacttt gctgaataga
     2581 aaggaagagc agtcaatgat tggaatcagt gcatgtttag gcggtctggc ctgtcgctat
     2641 gatggacaga aaaaagaagt accagaattg atggaactag ttgaaactgg agaagctgtc
     2701 atggtctgtc cagaagtgat cggcggatta ccaattccta gagatcccgc tgaaatcatt
     2761 ggtggaaatg gattcgatgt gtggaaagat caagcaaaag tatggacgac aagcggcgaa
     2821 gatgtaacgg aagcgtacaa aaatggcgcg atcaaagcct atcaaaaatt gcaggaaaaa
     2881 cacattgatc tagtcatcat gaaggaaaaa agcccttcat gcggaaccca ttcgatttat
     2941 gacggaagtt tttccggaat aaaaaaaaat ggtgccggag tggctactgc ttattttatt
     3001 cagcaaaaaa tgactgtgct ctctgatgaa gaatggctga aaactagagg tgagcagaat
     3061 ggaaatcgaa aagaccaatc gaatgaacgc gctctttgaa ttttattcga ccttattgac
     3121 agaaaaacaa atgaactaca tggaattata ttatgcagat gatttttcat taggtgagat
     3181 cgcggaagaa tacgaagtaa gccgacaagc agtatatgac aatatcaagc ggacaagcaa
     3241 gatcttggag gattatgaaa aaaaactcca tcttttttct aattatattg ttcgagaaca
     3301 ggtattggaa aatatgactg catatattac agataagtac ccggaagata aaaaactact
     3361 tgaatatgta cagcaaattc aagcaatcga agaataaata aaaggaatga agaaaatatg
     3421 gcatttgaaa gcttaactga acgtctccag caggcgatgg gaaaaataag aaaaaaagga
     3481 aaagtatcag aagctgacgt aaaagaaatg atgcgggaaa tccgccttgc cttactagaa
     3541 gccgatgtta acttgcaagt agtcaaagat tttactaagc gcgtacgcga acgtgcggta
     3601 ggggcagaag tgctggaaag tttatcacct gcacaacaaa tcgtaaaaat cgttgatgaa
     3661 gaattgacga tcacacttgg ttctgaaaca gcagagttaa ataaatcacc aaaaatacca
     3721 actgttatca tgatggctgg tcttcaaggg gcaggtaaaa cgacctttac tggtaaatta
     3781 gccaattatt taaagaaaaa tgaaaatgct cgtccgctcc taatcgcagg ggacgtatat
     3841 cgaccagctg cgatcgacca attgaaagta ttaggtcaac agctagacgt tcctgtattt
     3901 gatatgggaa ctgaagtcag cccggtagag atcgtccgtc aagggatgga gttagcaaaa
     3961 gaaaagaaaa acgactatgt attgatcgat acggcaggac gtctgcatat cgatgaaaca
     4021 ttgatggatg agttgaaaca aatcaaagaa ttgacgcaac caaatgagat tttgcttgtt
     4081 gtcgatgcca tgactggtca agatgccgtc aacgtagctg atagcttcaa ccagcaatta
     4141 ggtattacag gggtcgtcat cactaaactt gatggggata ctcgaggcgg ggcagcacta
     4201 tcgatccgct cagtgacagg cgcaccaatc aaattcatcg gttctggtga aaaattgact
     4261 gacttagaag tattccaccc agatcgtatg gctagccgta tccttggtat gggggatatg
     4321 ctgacattga ttgaaaaagc gcagcaagac tacgacgaga aaaaagcgga agaacttgca
     4381 cagaaaatgc gggaaaattc attcgatttc aatgacttta ttgaacagct tgatcaagtg
     4441 atgggtatgg gcccactgga agatctaatc aaaatgatcc caggaatgaa tcaagtccct
     4501 ggaatcgaaa acgtcaaggt agatccaaaa gatgttgaac gcaaaaaagc gatggtttat
     4561 tctatgacgc cagcagaaag agcaaatcct gatctgttga acccaagccg tcgtcgccga
     4621 attgctgccg gttctggaaa cagtgttgtt gaagtgaatc gaatgatcaa acaattcaag
     4681 gaatcaagaa aaatgatgca gcaaatgtcc aaaggggata tgaacatccc aggtatggac
     4741 caaatgtttg gttcaggcgt caaaggaaaa ctaggcaaaa tggcgatgaa tcgaatgatc
     4801 aagaaaaata agaagaaaaa gaaaaaacgg aaataagatg aaaaaaacag cttcggtaca
     4861 gactgaaaat gtctactgcc ggagcttttc tatcagcaat ttttattctt tactaataca
     4921 attactctaa acgaagaggt ggcacagatg agatgtccgt ggagtgaaca aacagaaagt
     4981 atgcggcaat atcatgatac tgtctggggt gttcctgaat atgatgacca aaagctgttc
     5041 cgaaaattga tgcttgatat taatcaagcc ggattgagct ggcagacgat tttaaataaa
     5101 agtgaagcat tcgatgaagc atatgatcga ttcgatatca aaaaagtcgc aaattatgga
     5161 gaaacaaaaa tcagtgaact aatggaaaac aaaggaatta ttcgtaaccg aagaaaaatc
     5221 gaagcagccg tccaaaacgc caaagcagtc ttgaaaattc aagaagaaat cggcagtttt
     5281 tcagagtatc tttggtcgtt ttctaatgga aaaatcgttg attcttctta tcattcacaa
     5341 gaagaagtcc caacaacaag tcctctttca gatgaattag caaaagatct aaagaaaaga
     5401 ggatttaaat ttatcggaag cacaacaatc tatgcttttt tagaagctgt cgggatcatc
     5461 aatgatcacc ttgacagttg tttccgcaaa gaagaggtcg tacgtatcgg cagaaagggc
     5521 gaataaagca tgcgaaaaat catattttat ggtgctataa gtttagatgg ctatttggca
     5581 acgaaaacgg atgatcttca atggcttttt gacactccaa caggtaagca aactacttat
     5641 gaagcttttt atgaaacaat cgatacgacc atcatgggca gaaaaactta tcaggaagca
     5701 aaaaaatatt tggagaccga agcaatttat ccagagaaaa agaatttcgt tttttccacc
     5761 cacgcacaat tacaattgcc agatgctgag gtcgtaaacg aagatcctgt tgcttttcta
     5821 aaaaatctac aaatgacaga aggaaaagat atctgggttg tgggtggcgg tcgattgatc
     5881 aaaccgctat tggaacatca aatgattgat gaatggtata tccagattac ccctgtatta
     5941 ttaggggacg ggattccttt attccaaaaa ggaacatatg aagcgcgttt tgaaatgcta
     6001 gacacgacac gattcggtga atttatcgaa ttgcattatg tacgaaagta gaagtaaata
     6061 aaataaaaaa ggcaactata tgcagaaatg tcctgtacat agttgccttt tttattaccg
     6121 ttatgctttt tactctgtta atttgttaga aaaatctaga tacgcatcta aaatcgtttg
     6181 gaagaaactt aatgttcctt caacgatatt accattttca tcaattagat tcgcaacgtt
     6241 tccgatatat gcttccggtt gctgcaaggt tggcacattc aaaaagacaa gtgattggcg
     6301 tagatggtgg tttgcgccaa agccgccgat cccacctggt gatgcagtaa cgactaaacc
     6361 tggtttatga tcccaaacac tgtgtccata aggtcttgag cctacatcta aagcattttt
     6421 caatactgct ggcacagaac ggttgtattc aggagtaacg aaaacgacac cgtctagtcc
     6481 cctcacttcc tcacgaaaac gagtccattc agcaggagcc tgttctggag tttcgagatc
     6541 ctcattgtaa aatggcagat catcgatttt tacaaataca ctttcgtacc cttcaggaag
     6601 caatttcgca aaagcttctg ctactttttt gttgtaagaa tcttttctta aactaccaat
     6661 aaaaaatcca attttttttg tcatacgatt tttcctcctt caaactatct ataataatct
     6721 tacaaaagat aagtaactgg agcaaataaa aacacttact gacaaaagct acatagaaga
     6781 tagattactt atttttaaca aaaaagaagt ctaaaatctt ctattttcta aaaaaaatcg
     6841 aatttaatca agctgtaaag aaaaaaagct ttacagggta aaaaaaaact gttacactac
     6901 atcttgtgaa taaaacaatg gaggtggaaa taaataatgg cagttaaaat ccgtttaaaa
     6961 cgtatgggtt ctaaaaagag tccgttttac cgtatcgtcg tagctgattc acgttctcct
     7021 cgtgatggac gtttcatcga aactgtaggt acttacaatc ctttgaaaga ccctgcagaa
     7081 gtagttttaa aagaagactt agttcttgac tggttatcaa aaggtgcaca accttctgac
     7141 actgtacgta acatcctttc aaaagaaggc gttatgcaaa aacaccacga agctaaattc
     7201 tcaaagaaat aaggtgaccg gatatgaaag atttaagcga cttagtctta acaatcgttc
     7261 gtccgctagt cacttatcct gatcaagtca agttagacgt cgtagagtcg aaagactttt
     7321 acgagtacaa tctaacagtg gctcctgaag atattggccg tatcattgga aaacaagggc
     7381 gtgttgctaa agcaatccgc acaatcgttt acggtgttcg gatcaatgca ccgaaaaaag
     7441 tgcgtttgaa tatcatcgat gataaggaaa agtaaataaa cgtgctgtac tcgaattttc
     7501 gagtgcagct tttttgtttt ttttattaat tgaagaaatg attatttggc aaaacaagca
     7561 agaaagcgta tccttatagg aggagggagt gaacagccaa tgagaaagaa aatgttattt
     7621 attccatttg ctctattgtt aagcgcttgt agttcggtta ctcaagaatc agacaataca
     7681 catctatcaa gcagtatatc tgaaactgtt accagtccag aaaaaagtag taccaaaacg
     7741 gcttccacca ctgaacaaac aatggctacc tcaaataatg agaaaaagac tgctttagac
     7801 cagttgaaag aacaacagcc aaatgttccc atgcctctag atgtaccagt ctcttcaggc
     7861 tatctcaata tcgcagcgac acatacaaag caaggttatt ctattttata ttacagaaca
     7921 gatcgtcccc tcgggttgaa tgctgacgaa ttgaatcaag aaacgcctat tgcaacctat
     7981 ctatatcaat atgggtttgc ttctagtcaa gaaacaatcc aagttttgca gccatttgag
     8041 atcgacacaa atggtcagca agtggactta ggttctcgaa tcacagggta tcagcaaggt
     8101 gcagcaggtt caagttttct tgaatggcaa gaaggaaact ggcgtattcg aatccgtggg
     8161 aataatatag aaggccaaga tcccttacta ttagctaaag aaatcgtagc ttatttggaa
     8221 gaaaacagtc taccagcacc tgaacaattt ggtaaaatta ctgttgatat gggtgatacg
     8281 acgaatcgag ccgtagaagt aagctggcaa gaaccgaaaa atgcctatac gatcactcat
     8341 caagatccta tgtctgcgtt gaagatggca gtttctatga agaggttgtg aaatcagcgc
     8401 gctgatctga gcccatcatt taactccaag tcacaggaag taatcaaaaa tagcacgccg
     8461 catttttgca taattttggc ttaatacctt aaaagaaaac tagaattgcc tcttcacaca
     8521 tgctaaaata aaacagacaa aaaagaaatg gggatataag ttgactgaat acttgaatgt
     8581 aggaaaaatc gtaaacacac aaggtatcaa aggcgaagtc cgtgttatct caacaacgga
     8641 tttcccagaa gaacgttaca aaaaaggaac ggtgctaaca ctattccaag acggaaaagc
     8701 acctgtggaa ttaacagtca aaagtcatcg caaacataaa aattttgact tattgagttt
     8761 tgaaggacat ccttctatca atgatgtgga aaaatatcgg gatggcatat taaaagtgtc
     8821 aaaagacaac tctgtagatc tagctgaaaa tgaattctat taccatgaga tcatcggtct
     8881 gcatgtatta gaagatggaa ctgagttagg taaagtaaaa gaaatcttgt caccaggtgc
     8941 caatgatgtt tgggtcgttc aacgtccgaa aaaagctgat atattgcttc catatatcgc
     9001 ttctgtcgtc aaagaagtcg atttagaagc tggagtggtc cgtgtagaaa ttccagaagg
     9061 actgattgac gatgaagatt gatgtattga ctttatttcc aagaatgttt gaaggtccta
     9121 tgggggaatc catcatcggt aaggctgtag ataaaggatt attagatatc aatatctcta
     9181 acttccgcga ttattcagat aacaaacatc agacagtaga tgattatcca tacggcggtg
     9241 gagcaggtat gctgctcaaa gtccagccca tctatgataa catccaagcc attgaaaaag
     9301 ctgcaccgga tgtcaaaaaa agagtaattc ttcttgaccc agctggtaaa cgattcgatc
     9361 agaaaatggc agaagaattt tcacaagaag aacacttagt attcatttgt ggtcattatg
     9421 agggatatga tgaacgtatc cgcacattag tgacagatga agtttcctta ggagattatg
     9481 tccttactgg gggagaatta ggtgcgatgg tcatgatcga tgcaacagtt cggcttttgc
     9541 cagatgtgtt agggaatcag acttcagctc aaacggattc ttattcaacc ggtttattgg
     9601 aacatcccca atatactcgc ccggcagaat ataaagggat gaaagtacca gaagtattga
     9661 caaatggaaa tcataaattg attgaagaat ggcaattaaa agaatcacta agaaggacct
     9721 atcaacgtcg gcctgatctt ttggaaacac ttgaatggac acctcaaatg acgaaatttt
     9781 tagaagaaat caagaaagaa gaacaagaaa aaacagcaga tgaagccaag tgactttttc
     9841 taaaatcttc tttacagtag gttgataata tgttagtatt ttatggtgag tgcaaaagca
     9901 cttaactatt acgatattcc gctgtgagaa aagctcataa gaatatttgg aataaggaga
     9961 agaaaaccat gaatccatta atcgaagaat taacaaaaga acaattacgt tctgacatcc
    10021 cagcgttccg ccctggtgac actgtgcgtg ttcacgcaaa agttgttgaa ggtactcgtg
    10081 aacgtatcca gttatttgaa ggtgttgtta tcaaacgccg cggtgctgga atcagcgaaa
    10141 cttatacagt acgtaaagtt tctaacggag tgggcgttga acgtacattc ccattgcata
    10201 caccacgtgt tgcaaaaatc gaagtagttc gctacggtaa agtacgtcgt gcaaaattgt
    10261 actacctacg tgcattacac ggaaaagctg ctcgtatcaa agaaatcaga agataaacac
    10321 ggaaaacttc cttggaaatc tgaaatttgt gaaaagtcct tgtatatcaa gggctttttc
    10381 ttttttctct atcacagttt taagccattt ttggctgttt tggaccattt ttcaatcata
    10441 ttcccttaaa aattgtccta gattgtccga cattgactat cagtgtcgtc aaaatagggc
    10501 aaaatttagt agtcagcttg acgacaaata taatacgaaa aataccaaaa catttaccga
    10561 atttagcaac ttttgaaccc aagtttctca aagggtcagc tattagcaaa aaagacaaaa
    10621 catgtacttt tctactaaaa agttgatgtg ataggcttct atctaaataa ctattaggag
    10681 tttttatcgt gaagcaaaat gatggacgta tcatttggaa aaatcaaagt gagttaaaac
    10741 tgattttaac aattaatgaa ttcatcgaaa aacacggaat tacatctagc cgtcaatacc
    10801 agaaaaagct atcagaaaat ccaaattctg caccaagtat gtggttcatt aacaaaaaat
    10861 atggatcatg ggagaattta ttgattagta taggacgaga aaatactggg tatggaaaat
    10921 gggctagaat gtcagaacaa gaattattgg aaatagtaga agcctttatt aagtgcgaga
    10981 agataacctc tcaacgaatg tacgaacaaa aatcagtagg gaagaatatt ccttcactta
    11041 gtaccattaa aaagatgtta ggagatatac gtccactttt taaagaaaaa aatgatggat
    11101 ctcgatttac tgatttcgaa ctgctgttag aactaaaaaa tgaaataatc cgattaaaac
    11161 tacaggatga tttatcaatg acaaagtttc gaaaactagt tcaatcccca aaactaccat
    11221 cagttgatac aattatgaaa agaacgaaca aaaattggga ggaattgatg gcagagattg
    11281 gatttgatta tcgaagaatc aaaatttata agcagagaaa caacctatcc aagacaaaga
    11341 aaactaaata gtatcactct aaatcagacg ttcaaaatta aaatttggac gtctttttta
    11401 ttacccaaaa acgaaaggag atttatttta tgaaaaagcc aagatttaag aattggcgtt
    11461 tgctcgcaac actgagtcta ttatgccaaa caattggtgg atcgcttggc ccaacgattg
    11521 cctttgctga tgaaattact catccacaaa cggtaactgt ggaattggat ttggctcacc
    11581 agtatgctgt cgaaggtaca tttagcgatg gtcgtcccat gtcagaagtt accgttccgc
    11641 actatgcagt ttacaatggt gtgaagcaag atattttctg tattgagcca ggggtgccga
    11701 tttacaatga gttcactcct ggttatgaga aaaatccatt gcctgatatg tcggagaaag
    11761 cgaaattagt ttccgtttta tggaaggaag ctggtacgga tgtggataca catattgtgg
    11821 cccagaagat gatttggcaa gaagttaatg gctacacgct tcactcaatt aaacgttctg
    11881 atggtagtgc agtaaatatc gcagccattg agtcaaaaat caatcaagcc attgcggatt
    11941 atcaaaagaa accaagcttc cataactcca cagtgaaaac cgtgttaggt caatcgacta
    12001 ctgtgacaga tacgaacggc ttgaatttat cggagtttga tgaagttgtc gagaatatcg
    12061 caaatattga ttatcgtgtg aacggcaatc aactagtgat tactccaaat gccaattcaa
    12121 aagagagtgg tgaattaacc ctaaagaagt cagctaatac aggaacaccg gtagcttata
    12181 aaatggctgg tcaacaaact ttgatggcgg gggcaattga taagcccaat acctacacaa
    12241 tcaaaattga tgtggaaact gagggttctt tgaagattaa aaaagtcgat aaagaaacag
    12301 gaaatgttgt accgggaacc gttttccatt tagactttgg aaaaacatta cctgcaaaag
    12361 acgtgaccac cgataaagaa ggcattgcga cattggatga gattcctcat ggcgcgaaag
    12421 taaccattac tgaaaaatca gtgccagctc cttatacgat tgacactaca ccaatcaccg
    12481 caaccattaa atctggtgag actatttctg tgacttcaaa aaatactcgc gaaaaaggtc
    12541 aaatcatttt gaataaaatg ggggtggaaa caggaactga tctttggaac gacaaatact
    12601 cattagccgg aaatacgttt gccattcgca aagacagtcc aaccggtgaa attgtccaag
    12661 aaatgaccac ggatgaaaaa ggtcatgcgg aaacaccaaa agagattgcc aatgcgttgg
    12721 aattgggaac ctattatgtg accgaaacca aagctagtaa tggctttgtg aataccttta
    12781 aaccagtaaa agtcgaattg aactatgtca atcaaaccgt ggctcttgtt accagtaacg
    12841 taaaagggca aaaccaagaa atcactggtg aaaccacttt gacaaaagaa gacaaagata
    12901 ccggtaatga aactcaaggt aaagctgagt ttaaaggagc tgaatatact ctctttactg
    12961 caaaagatgg tcaagcggtc aaatggagtg aggcttttaa accagagtta gtgaagggaa
    13021 cgaaagcttc tgatgaaacg gtgactttga ctttagatga aaagaatcaa gttgctgtta
    13081 aacacttagc gattaatgag tattactggc aagaaaccaa agcacctgaa ggatatacct
    13141 tggatgaaac aaagtaccct gtttccatca aaaaggtgga tgataacgaa aaaaatgccg
    13201 tgattacccg agatgttacg gcaaaagaac aagtcattcg ctttggcttt gatttcttta
    13261 aatttgctgg atcagctgct gggactgccg aaactggctt caatgatctg acctttaaag
    13321 tatcgccatt ggaaggaacc agtgagatta caggtgccga agatgaagca attacagctt
    13381 ataacgagca actaggtttt gatggctatg gcaagtttga aaatcttcct tatggggatt
    13441 atttacttga agaagtagag gctccagaag gatttcaaaa aattacgcca ttagaaatcc
    13501 gttctacttt taaagaaaat aaagcggatt acactaagag tgaatacatt tttacgatta
    13561 ccgaaaaagg acaaaaacaa ccaattaaaa cggtgaccgt tccttacgag aaactgacga
    13621 ataaagcatt ttctgttagc ttgaatcgtt tgatgctcta tgatttacct gaagaagaag
    13681 atagcttaac ttctcttggg acttggaaag acggaggcaa agaattgaca gaccttgatt
    13741 ctaccgagct agttgataaa ttgagctata acttgcatga aatcaaagaa gactggtatg
    13801 tggtagcgca agccattgat gtggaagcga caaaagctgc ccaagaaaaa gatgaaaaag
    13861 ccaaaccagt ggtaattgcc gaaacaaccg caacgttggc gaataaagag aaaacaggaa
    13921 cttggaaaat tttgcataaa ttaaccgcag aacaagtttt ggataaaacc atcgtcttat
    13981 tcaactatgt gtatgaaaac aagaaaccct atgaagcagg tgatgatcca gtagcgaaag
    14041 atgctagctt gaacaatcaa gcccaaactg tcaattgtac ggtggaacgc catgtttcca
    14101 tccaaacaaa agcccaccta gaagatggtt cgcaaacctt tactcatggg gatgtggtgg
    14161 atatgttcga tgatgtgtcg attacccatg atgtactgga tggctcaaaa gaagcttttg
    14221 aaacaattct gtatgcatta cttccggata atacgaccaa agaaatttgg aaatctggca
    14281 aaattgatta tgaagtgaat gacaaagaat tcactaaaac cgtacttgcg gaaaaagtgg
    14341 acacaagtaa atatccagaa ggtacaagtt ttaccttcaa agaaattaac tatggcaaag
    14401 acggaaacat caatgggaaa cacaatgagg atctaaaaga aaaaactcaa actttaacac
    14461 ctaaaaaagt tccgacaaca ccaagtgcac cagaacaacc ggaaatacca actgttccaa
    14521 gtgactctca agaatctagt cctacagtaa agacattccc acaaacaggg gagaaaaatt
    14581 ctaatgttct gctgtttatt gggtttacct tgatctttgc gactgctggg tattatttct
    14641 ggaatcgccg aaactaaggt gatgtgatga aacaactaaa taggaaaaac caacgtgcgc
    14701 gaccgcctcc taaaaaaagt ggtcgcgcta tttttgtatc caaaactaaa aatgaaaaga
    14761 ggaaaagaag atggaattga aatttgttgt gccagatatg gccgaaacct ttggaaagat
    14821 aaggtatgcc ggcgaaggcg aagttttaac tgaaggatat gggcggaata ctacagtaat
    14881 tggtcgtagt tatcatttgt attccagtaa gcaacgagcc gatgatattg aagtggtggt
    14941 agctgcggaa gccggcgaaa aggattttga tcaagatcaa ccactaaaag ccgtgaatcc
    15001 ccatttggtt gccaaaggct atgagattga gaatcgtggg tttaccgact acgtgttgta
    15061 tgtcgatgat ttagtgaaag cataggagga aaagaagatg agattaacag aaggtattgt
    15121 tgtagattca ggactaacgt ttgggaaatt acgattttcc gcattacgcc gagaagtacg
    15181 gaagcaaaat gaggatggaa cggttagtaa cgaggtaaaa gaacggactt ataatttgaa
    15241 atcctctgcg caaggtcgaa tgatccaagt tagtatccca gcaaatgtac ccttacgcga
    15301 gtttgcctat gacgcagagg tagagttggt caatccaatc atggatacgg tcgccaatta
    15361 tgttttccga gaaggaacta ctgtcaattg gttcattaag gccgatgatt tagtcttgaa
    15421 acgacaacca gatcagggaa attctacaaa ccaaaatgaa ggaaagaaat aggtgaagcg
    15481 tatggaaatt aatgttgaac atattttaga ttgtctcgat caatatggaa aaggtgagct
    15541 aacggaggaa caactcacta aagcattaac ctatgatgaa aaaatgtttc tgattatgca
    15601 tcaagggtta ctggaagtag gtaacgatgt cgaagaagat tttgatgtac tgaattggtt
    15661 aggggaagaa gagtccttct ttatggttat cgaagtaaat gaaagtctat gtcgagaagc
    15721 cgaaaccgta ttggaagaaa ttggtgtgga aatgccggat gccattgaag tttttttaaa
    15781 gcaactagtg gaaacgaaac aactccctat agcagtaaat gactagcata actgcttgaa
    15841 attggatcaa tgaaatccta tgaagctttt ttcgattgga ggtggaaacc ataaaaatgt
    15901 acaaagggca tcgtatacgg gcaggagatc agcacttggt ttatcacttt gttcttggct
    15961 ggctactcgc tctgttcatc ggttggatga gcgtctttta ttttcaagag cttagacaat
    16021 ttgatatttc taagctatca ctttctacta tagaaatcgt tcgttctata aaagatttaa
    16081 tctatttgct aggaagtctc gttctttcag gaagcacgat gttgctttat atacactttt
    16141 ttcaggatca ttggcgcagt ttgtggcatc gacaaaagct agcccggatg attctagaaa
    16201 atcattggta cgaagtaaaa caaactcaga gtgaaggatt tttcaaagac ctcaatagta
    16261 gtcgaaccaa agagacaatc agttacttcc ccaaaatcta ttaccgtatg aaggatggtt
    16321 tactttctat tcgcgttcaa atttcactgg gaaaatatca agatcagctt ttaaagctgg
    16381 aaaagaagtt agaaagtggt ctatattgtg aactagtaga aaaggaactc aaagactcct
    16441 atgtggagta cactttactg tatgatataa ttgccaatcg aattgggata gacgaagtag
    16501 tggcagaaag tggggctttg cgattgatga aaaatcaagt atgggcttat gattctttac
    16561 ctcatatgtt aatcgctggc ggcacaggtg gtgggaagac ttacttttta ctcactatca
    16621 tcgaggcttt attaaaatct gatgccgagc tttttgttct tgatcccaaa aatgcggact
    16681 tagccgattt aggtacggta atgcctcatg tctattccca aaaggaggaa atttctgctt
    16741 gtgtggaaga tttctatgag cgcatgatga ctcgtagcaa ggcaatgaaa gaaatgccca
    16801 actacaaaac aggagagaat tatgcgtttc ttggacttcc accaaacttt ttaatctttg
    16861 atgaatacgt ggtttatatg gaaatgttaa cgacgaagga aagtgcggtg attttgaata
    16921 agctcaaaca aatcgtgatg ttaggtcgtc aatctggttt ctttctgatt ctagcttgtc
    16981 aaagaccgga tgcgaaatat ttaggagacg ggattcgcga tcaatttaat ttccgggtgg
    17041 ctttgggtcg tatgagtgaa ctgggatatt ccatgatgtt tggcgaggtt gataaaaaat
    17101 tctttatgaa gcgtatcaaa ggtcgaggct atgtagatac gggagggagt gtgattagtg
    17161 agttttatac gccacttgta ccaaaaggat atgatttctt aagggaaatt agcgacattg
    17221 gtggattaaa cgttaatacg gaaggataaa ataatagtat gtagcctagg agtgtcaaga
    17281 atcctgggct ttttttacga ggtgatttat gacattttta gatttattcg ctgggattgg
    17341 tggcttttgc ttaggtatgg aacaagcggg tcaccagtgt atcggctttt gtgaaattga
    17401 tgattttgct cgccagagtt ataaggcaat ccatgataca agtaaggagg tggaaatgca
    17461 tgacatcaca agtgtatcag acgagtttat tcaatctctc ggaccagtgg atatcctttg
    17521 cggtggattt ccgtgccaag ctttttcaat tgcgggaaag cggcaaggat tttccgatac
    17581 tcgaggtact ttattctttg aaatcgctcg gttcgccgct attctccaac ctaagttttt
    17641 attccttgag aacgtccggg gattactcaa tcacgaagga ggggctacgt tcgagacgat
    17701 cctccgaacg ctggatggat tggggtatga tgtggaatgg caagtgctta actcaaaggc
    17761 ctacgttccc caaaaccgtg agcggatctt ccttatcgga cattctcgag acgcatgtac
    17821 cgaacaagta tttcctatca ttggatcgtc tccaacatct gatcaaaaca tcagaaactt
    17881 attaaatatc aacccttcca atcgaggaat ggggggacaa gtctatgggt cagatgacgt
    17941 agcgcctacc ttgaccggtg atgaaggaat caaaatcgca ttgccggtag atgatggaat
    18001 ttctgtagcg ggtatgttac tgggaaattt tgaacaaggc aatcgggttt atgagataac
    18061 tggaacagcg cctactttgt caacgaaaca aggcggaacg aagatcatgc ttcggaaaaa
    18121 cgaaacagaa tatcaagagg tgaagccagg gaacagtgtg aacctggctt ttcctaattc
    18181 cacaagtcgt cgaggccgtc tgggaaaaca aagtgtgcat accttattaa caggagacca
    18241 acaagcagtc gtcacggatc agtatcaaat tcgaaagtta acacctaggg agtgctggcg
    18301 gcttcagggc tttccggact gggcttttga tcgagcttct caagtcaatt cagatagtca
    18361 gttgtataag caggctggaa attctgtaac agttccggtc atttttgata ttgctagaag
    18421 attaaaggag gtaaaaacag atgatttatg atgagttgat tggggaatct actgggtgat
    18481 tggcaagatt cagtcagatc cggaattgga agaagaactg taccggctca attttgatat
    18541 tcgcaaaaac ggggtgaaag tccctgatga tccgtatgta attgatgagg aaacggatgc
    18601 tcgaatggag ctgaatcaag tcatcagtga attggaacga attgcggact ttgctaaaga
    18661 acctgatatc cgccaatact tgtttgagat aaaagcagag ttggaaattg agggtattac
    18721 cgcggagtaa cggccaaata aaccactgat aggcactgac cgcgggggat ttgcgaagca
    18781 gaaatcctcc gtggtgctgt gcctgtttat ggacggcgtt agccggccat gatgtctcac
    18841 cccccgtatc taacaggggg gtacaaaaac acaaaaaata agcaagcaac gataaggaat
    18901 ggctgtccta tcaaggataa taagcaattt gtgtagatgt taactgattt gaaaaagtta
    18961 acaccttaaa tttggaggtg agaacttggc acaaaggaat ttagattatc ggctattaaa
    19021 agaccggcgt aatgaatatg gcgtttcaca aaataaatta gctacgactt gtggacttag
    19081 tcgcccgtac ttaaatcaga tagaaaatgg tggcgtaacc gcatcgacaa aaaccatgag
    19141 gaaaattttt gaccaactgg aaagtttcaa tcccgagtta cctttaacgt tactgtttga
    19201 ctatgtaagg attcgctttc ccacaacgga tgcgaggaaa atcattcaag agattctcca
    19261 tttgaaattt gattatatgc tccacgaaga ttacgccttt tactcctatc aagaacaata
    19321 tgttatggga gatattgtgg tgatgttgtc acatgaagaa gataaaggcg tccttttaga
    19381 attaaagggt cgtggttgtc ggcagtttga aactttttta ctcgcccaaa agcgtagctg
    19441 gtatgacttt ttcgaagatt gtctaaaagc cggtggcgtg atgaaacgct tagacctggc
    19501 aatcaatgat cgagttggcc tgttagatat tcctgattta acgaaaaaat gtcagaagga
    19561 agaatgtatc tccttgtttc gtaccttcaa aagctatcgt tccggggaac tgttgaaacc
    19621 tgatgataaa gatggtatgg gcaatacttt gtatattggc agtcttaaga gtgaggtcta
    19681 tttctgtttg tacgaaaaag actatgagca atatatcaag ttagggattc ctttagacca
    19741 gaccgaaacg aaaaatcgtt ttgaaatccg actgaaaaat gatcgagctt atcatgcaat
    19801 tcaagatttg ttaaaaggtc gcagtattga aagtaccaca ttttccatta ttaaccggta
    19861 cttgcgattt gcggataagg tggaaggcaa aaggcgaact aactggccct tgaatgaaca
    19921 atgggggcgc ttcattggac ggaatcagaa ggaaattcaa ttaacttctg aaccgaagcc
    19981 ttataccatt gaacgaacgt tgaactggct tggacgacaa gtcgccccta cgtggaagat
    20041 ggccaaggag ttggatcggt tgaatcagac aacgtacatt caggatatgg taaataatac
    20101 tcgactatca gaccggcaca aaaagatttt ggagcaacaa agtatggcaa ttgaaaattt
    20161 gattatatga aaagaggaat cgtgatggaa attgtgaaag atattttatt ggtgtttggt
    20221 ggcagtatgc ttggtgttac tgtgatgtgc ttgatgcatg caagtgcagc ggcggaccga
    20281 gcaatggaaa agaaaggaaa gtgatgaaat gaatttcggt caaaattt
//
LOCUS       DANKKG010000042        20217 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000042 DANKKG010000000
VERSION     DANKKG010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.042"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            133..636
                     /locus_tag="PW004_001276"
     CDS             133..636
                     /locus_tag="PW004_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDL2726261.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKEQIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCNLAEELEGTPIGEAASEIQHAFFNSFEEMVEH
                     VDDIVYYPDCDDMSDVAHYLIVEAGDFGEVPEQLKPYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPE"
     gene            763..1800
                     /locus_tag="PW004_001277"
     CDS             763..1800
                     /locus_tag="PW004_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726262.1"
                     /translation="MANIQTSFIDFHNSIRLDVEDNTLLKDYKDQVIDGLKDYLPDDV
                     KFETFLQGSYSVYTGIKSCDEKIDFDIDIAVAFEIDHTVYEDPREPKLWVKEALVEIF
                     PNAQVNLKVPCVTATFTGKKTKKNVHVDVAVYAKEDENYFLAKAKEFSAPENRCWEEA
                     DPKVLKEKINSHVADSDDRKQFRRCIRYLKRWKDNNFNQEYKPTGIGLTINVMDTFLV
                     NKSTDFLTRKVQYNDMECMKQIVSSLKDSFVYEYSETDGWHYRLHAYLPVKPNSDTYS
                     KMTVNQMSDFKNKLSKLYDDLIFAIDTEDEYEATKRLNNQFGEDFLIISEEEVTEKNL
                     RNAFVTDYPSA"
     gene            1815..3512
                     /locus_tag="PW004_001278"
     CDS             1815..3512
                     /locus_tag="PW004_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HDL2726263.1"
                     /translation="MDYTDILKEKLTDVVRLFDGNYYGDFSFATIGKLRLKIEIPKHF
                     PLVLPRIYIHNFDHLGLYLPHVEKDGLVCFSSDINYLVDEENSELVLLQCVNKTIKTI
                     EEGLKGLNKNDFRKEFVAFWDKQENQISLFYLCEPALFIKSVDTIMYGKSLVALDKEN
                     ELQLNAASRLFNGEKEDTHELDAYYVPIRNKNNIMPPKNNKAWSKKDILQIVKKNVTG
                     ANKRFFLKLEKRKNRHNVLLILDIPIEEKSNNVIIGFLFTKQSGSIKDYKGTPRTVLV
                     QRHDELYLVNRTSGENYGLGANIAIVGVGSVGSKIAENLGPLGVRKMTLIDNDCFQAE
                     NLYRNSLGADSLKNARSQNNFFKVDLMQEELEKKIPYLCVEVESMNVLDLISMQPGFF
                     KEYDYVFVCIGDTMASLALNNYFSKSSQKVFYSWLEPYGIGGHTLFINYSLNQKGCFN
                     CLYTDSNSKNRCSNRASLVKEGQIFERTLASCRSKFIPYNAIASTECAINAIKLFHKV
                     NQNNMTNSTLFSWIGDGSTFENLGYQYSDRNTFLNGVNKFEERFFSKYCKVCGELDD"
     gene            3505..3966
                     /locus_tag="PW004_001279"
     CDS             3505..3966
                     /locus_tag="PW004_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HDL2726264.1"
                     /translation="MIKFSCDRIVIEVRDEVLKIFEQYRQGKDDLPEAGGVLHGKRLK
                     NGSIIITDITTPQIGDIQKRLLFKKNREIHQQLSNEKWIESNKFSIALGEWHTHPEAV
                     PTPSNVDKKSWRLNVSKQHDDRVYVFIIVGFSDLRMWLLSKKSDIRQAIKL"
     gene            3979..4839
                     /locus_tag="PW004_001280"
     CDS             3979..4839
                     /locus_tag="PW004_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726265.1"
                     /translation="MKSQKNIWWWGFLVGKVMIPALLLISLPMITFFFTNKKMSKEAV
                     IFFFGDQKATFIETLVTSLQLNLNYIFSIIIVISLLNFFKKRNSGKVFNSNGNVYYNY
                     FYFVFWVAATLLGYDKIQIAGIPIHMQYKLVLSGIFSEVLPDIYDDHYDSDGTCKVSI
                     EKENFDDIDGYDSVNLLIIDTYDIKMSELSMENQTYPTIIVRGNSIDGVRKVNRSLIL
                     EIKKTMDEIQKSDFKKVFVASTSNPKNSINIINSSFRFFGRSRRFKLYVLQKDYASNG
                     KYSKKYRIFI"
     gene            4877..5266
                     /locus_tag="PW004_001281"
     CDS             4877..5266
                     /locus_tag="PW004_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL2726266.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLMFV
                     IVFAHVPPLSMIEGAFLKYLGIPIAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            5250..7700
                     /locus_tag="PW004_001282"
     CDS             5250..7700
                     /locus_tag="PW004_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL2726267.1"
                     /translation="MMYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKYQVHD
                     NFRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDLQTDALVSM
                     IGDSQIDYRFFIGFKLISTDEEVNLKNLRKSFFSGLQEFVYGVNHHLMGDFVSLSNEE
                     IRRYTKLEKLMESKLARRFKVKRVTPSDLTYLIEHIYGEKGTPFEEYEFQLPKKKLKS
                     ETLVKRYDLLRPTRCLIEEKPRYLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQ
                     QQQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSV
                     DELETTLDQSKESMYKLSYVVRVSAKSVDELKRRCDEVLDFYDDTNVKLVRPFGDMMG
                     LHKEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKP
                     ALASQGVKGSVTNALASAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWK
                     ETLPEIADEINILNLTSKPENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEK
                     FPVLRKAIRTVTQSDQRGLLQVIVELRKEDSPVAENIADHIESFTDYDFAHLLFSDGS
                     ITQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNRE
                     IFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGL
                     KFAFRSTDIHEIKKTLEFFGLDKEDESNQKCMRDLENGQCLMQDLYGRVGVVQVHPVF
                     EELFHAFDTRPPVQERME"
     gene            7705..9813
                     /locus_tag="PW004_001283"
     CDS             7705..9813
                     /locus_tag="PW004_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDL2726268.1"
                     /translation="MKKKLFRIFGIVLISLVTLIILLSLVGTVAEATGLVDDTVGAGN
                     LYSQYSLGNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEVGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMNMFNLQSNDSQSMGRRVMRKPQMLMNRTLR
                     QLNRNVGRTLAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEIPSDLNKEK
                     PIPNNKKQSRMNLAGRKVGKVLDTQALVKDKTKQVKDGVRNTPTNLKYNFHKGIEKTK
                     KAPEEFKRGLVQEKANRGELREKQQQRRDEKIAEKRKVLNEIGNPHGKRRENVPIKAK
                     VQSQKDRAPKRIVRANSNPEIKRKFASQEIISKGINQSSSKKNSFQQVKRRSTLSKRT
                     NRKVQKSLNPHPKPRSGEKK"
     gene            9810..10814
                     /locus_tag="PW004_001284"
     CDS             9810..10814
                     /locus_tag="PW004_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HDL2726269.1"
                     /translation="MNVKKLSAISLTFLTISFMGILMCVGLLFGEDSEGSSSSGAPPG
                     GSSVSEEVLKHRAMVEKYAKESDITEYVPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNFGGGYLDYVAKNGKKHSFALA
                     ESFSKEKSCGQKIDYPNPIANKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSRPETGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTESYWQQHLIGAGRYK
                     "
     gene            10833..11744
                     /locus_tag="PW004_001285"
     CDS             10833..11744
                     /locus_tag="PW004_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL2726270.1"
                     /translation="MKIKIERNQKEKSLKKKKERVVSVGKHRKMVLALWLLLSCSLTF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGIENFTKDFSKEYFSWKNNKEAIEKRMNNL
                     EQYLTEEGFALSQDMVRADIPTNSEVQSVKVLDVEKSSEEFVVSFLVEQKITEGKKAQ
                     SISSAYRVTIFEDENRNYIVTSLPTMIAKPDRAKYKTKQVENDSKIDAKTTEEIAEFL
                     ETFFKLYPSASEKELEYYVENDAMRPINIDLKFSKLEDPVYLQNGKKILVSTKIKYLD
                     NVSKTTSYFQYKLILQKNGNWKIIDVK"
     gene            11931..12218
                     /locus_tag="PW004_001286"
                     /pseudo
     CDS             11931..12218
                     /locus_tag="PW004_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(12406..12621)
                     /locus_tag="PW004_001287"
     CDS             complement(12406..12621)
                     /locus_tag="PW004_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726271.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(12880..13047)
                     /locus_tag="PW004_001288"
     CDS             complement(12880..13047)
                     /locus_tag="PW004_001288"
                     /inference="COORDINATES: protein motif:HMM:NF018933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2726272.1"
                     /translation="MGSECGHTDGKKSFTIREWTCPICRTHHDRNVNVSINILIKGYV
                     YKHCLRKHQEP"
     gene            complement(13392..14831)
                     /locus_tag="PW004_001289"
                     /pseudo
     CDS             complement(13392..14831)
                     /locus_tag="PW004_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(15379..16050)
                     /locus_tag="PW004_001290"
     CDS             complement(15379..16050)
                     /locus_tag="PW004_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HDL2726273.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRYLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCQCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEKWIAYITLK
                     KRGTI"
     gene            complement(16399..17850)
                     /locus_tag="PW004_001291"
     CDS             complement(16399..17850)
                     /locus_tag="PW004_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HDL2726274.1"
                     /translation="MKKSLKLASAVILMSQLFIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTLSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSAESSTTSSSTSEITSSKVTRPNHIESSKKEAPKTEGQTKNFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            complement(17962..19305)
                     /locus_tag="PW004_001292"
     CDS             complement(17962..19305)
                     /locus_tag="PW004_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL2726275.1"
                     /translation="MKKIVIIGSNHSGLAAARFLNLCDSNYEVILIDKKSEISYLECG
                     TSLFIKREVSNLEDLFYQNRRELNSKIKKLFLETAVDKIDIQNKKIICVKKDGTRFSQ
                     EYDKLILATGSKQIMLDVPGNNLANIFSIKNFPNAKEIFKALDDNTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGKKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHIDNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPVFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            complement(19439..>20217)
                     /locus_tag="PW004_001293"
     CDS             complement(19439..>20217)
                     /locus_tag="PW004_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL2726276.1"
                     /translation="IEVTYPDGTKDTVKVPVEVTDNRSDADKYNPEGQKVTTDLNKEP
                     DASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGIVVVTYPDGSKEEVEVTISVEDKK
                     APNKPQVDPITEGDQIVTGKTEPNAEVTVTLPNGSQYHGTADKNGNFTVKVPKLEAGT
                     KVIVTATDESGNTSEPTNVVVSSNEKDSEKAVSKDNKTDNQGSKQNKNRGKSSPQKQS
                     SKAYPKTGEIDSNIFTISGGLILLGTLGLLGYKNRKKENE"
BASE COUNT         6518 a         3434 c         3759 g         6506 t
ORIGIN      
        1 gagttttcca aactgattgg cttcttggtg attgctttga ttgcggtagg tctggtcttt
       61 aatgcttctg gtgtgaagga tgtcctactc aatttgttta accgaattgt cggtgcctga
      121 gggggaatca caatggaaca gatgcgtgtt tatattgcga accttggaaa atacaatgaa
      181 ggtgagttgg tgggggcatg gtttacccca cctgtcgatt ttgatgaagt gaaagaacag
      241 attggattaa atgacgaata tgaagagtac gcgattcatg attatgaact gccttttgag
      301 attgatgagt atacgccaat tgaagagatt aatcggttgt gtaacttagc ggaggaacta
      361 gaaggaacgc caattggtga agctgcttct gagattcaac acgctttctt caattctttc
      421 gaggaaatgg tagagcatgt ggatgatatt gtctattatc cagattgcga tgatatgagt
      481 gatgtggcgc attacttgat tgtagaagct ggtgattttg gcgaagttcc agaacaattg
      541 aagccgtata ttgattttga tgcttgcggt cgagatttgg agattggagg aaattatttg
      601 gttactagtc gtggagtttt tgagtatcca gaatgaatca aacactatat atagtgtttg
      661 attagtatat gtaagcaaaa aaacacaata tatagagtgc aaactgaagg gtaatgtgat
      721 ataatatttg ccgaagaaat atttttgaaa tgaggtaata ttatggcaaa tattcaaaca
      781 agttttattg atttccataa ttcaattaga ttggacgtag aagacaatac gctacttaag
      841 gactataaag accaagtaat tgatggatta aaagattact tacctgatga tgtgaagttt
      901 gaaacttttt tacagggaag ctattctgtt tataccggaa taaaaagctg tgatgaaaaa
      961 attgattttg acatcgatat tgcagttgct ttcgaaattg atcatactgt ttatgaagat
     1021 ccaagggagc ctaaactgtg ggtaaaagag gcgctagttg aaatttttcc taatgctcaa
     1081 gttaatttaa aagtaccttg tgtaactgct acatttactg gaaaaaagac aaaaaaaaat
     1141 gtgcacgttg atgttgcggt ttatgctaaa gaagatgaaa attatttctt agcaaaagca
     1201 aaagaattca gtgctcccga aaaccgatgc tgggaggaag cggatcccaa agtattaaaa
     1261 gagaaaatta atagtcatgt agcagattca gatgatcgaa aacaatttcg aagatgtatt
     1321 cgctatttaa aaagatggaa agataacaat tttaaccaag aatataaacc aacaggaatt
     1381 ggacttacaa ttaatgtgat ggatacgttc ctagttaata agtccactga ttttttaact
     1441 agaaaagttc agtataatga tatggaatgt atgaagcaga ttgtttcttc gctgaaagat
     1501 tcatttgtgt atgagtacag tgaaacagat ggttggcatt atcgtctaca tgcatatctt
     1561 ccagtgaagc ctaacagtga cacctattca aaaatgacag tgaatcagat gagtgatttt
     1621 aaaaacaaac tttctaaatt atatgatgat ttgattttcg ctatcgatac tgaagatgag
     1681 tatgaagcta caaaacgatt aaataatcaa tttggggagg attttttaat tatttcagaa
     1741 gaagaagtta cagaaaaaaa cttaagaaat gcatttgtta cagactatcc gagtgcttag
     1801 gttggtgtta aagattggat tatactgata ttttaaaaga aaagctaaca gatgttgtgc
     1861 gtttattcga tggtaattac tatggggatt tttcatttgc tacaattgga aagttgcgtt
     1921 taaaaattga aataccaaaa cattttccat tggtacttcc taggatttac attcataatt
     1981 ttgatcattt aggactttac ctaccacatg tagaaaaaga tgggttggta tgtttttctt
     2041 cagatataaa ttatttagta gatgaagaaa attcagaatt ggttttattg cagtgtgtta
     2101 ataaaactat aaaaacaatt gaggaaggtt tgaaagggtt aaataaaaat gattttcgaa
     2161 aagagtttgt tgctttttgg gacaaacaag agaatcaaat ttcgttattc tacctttgtg
     2221 aaccagcgtt gtttatcaag tcagttgata caataatgta cggtaagtct ttggttgctt
     2281 tggataaaga gaacgaatta caattaaatg cagcgagtag attgtttaat ggagagaaag
     2341 aagatactca tgaattagat gcatactatg ttccaattag aaacaagaat aatattatgc
     2401 ccccgaagaa taataaagca tggtcaaaga aggatatttt acaaatagta aaaaaaaatg
     2461 taacaggtgc aaataaaaga ttttttttaa aacttgaaaa aagaaagaat agacataatg
     2521 ttttacttat tctagatatc ccaattgaag aaaagtcaaa taatgtcata attggatttc
     2581 tttttacaaa acaatctgga tctataaaag attataaggg tacgcctagg actgtattag
     2641 ttcagcgtca tgatgaatta tatttggtga atagaacgag cggagaaaac tatggattgg
     2701 gtgctaatat agctattgtt ggggtagggt cagttggcag taaaattgct gaaaatcttg
     2761 gtccattagg tgtgagaaag atgacactca tagacaatga ttgttttcaa gctgaaaatt
     2821 tgtatcgaaa ttcacttggt gcagatagtt taaaaaatgc ccgatctcag aataattttt
     2881 ttaaagttga tttaatgcag gaggagctcg aaaaaaaaat cccatacctt tgtgttgaag
     2941 tggagtcaat gaacgtttta gacttaatta gtatgcaacc tggttttttc aaggaatatg
     3001 actatgtttt tgtttgcata ggagatacaa tggcaagcct ggctttaaat aattattttt
     3061 ccaaaagtag tcaaaaagtt ttctattctt ggctggagcc atatggcatt gggggacaca
     3121 ctctatttat aaattattct ctaaatcaaa aaggttgttt caattgtctt tatacagatt
     3181 caaactctaa aaataggtgt tcaaatagag cttctctggt aaaagaagga caaatttttg
     3241 aaaggacttt agcgagttgc cgctcaaaat ttataccata taatgctatt gcctcgacgg
     3301 aatgtgcaat taatgcaata aaacttttcc ataaagtcaa tcaaaataat atgaccaata
     3361 gtactctttt ttcatggata ggagatggga gtacttttga gaatttagga tatcaatata
     3421 gcgacagaaa tacttttctt aatggagtca ataagtttga agaaagattc ttttctaaat
     3481 attgcaaagt atgtggggag ttagatgatt aaattttcgt gtgaccgtat tgtcattgaa
     3541 gtaagggatg aagttcttaa gatatttgaa caatatcggc agggaaagga tgacttgcct
     3601 gaagctggag gagttcttca tggaaaaaga cttaagaatg ggagcataat aattaccgat
     3661 ataacaacac ctcaaatagg agatatacaa aaaagattac tctttaaaaa aaaccgagaa
     3721 attcatcaac agctttctaa tgagaaatgg atagaatcaa ataagttttc tattgcattg
     3781 ggagaatggc atactcatcc tgaagctgta ccaactccat caaatgttga taagaaaagc
     3841 tggagactga acgtgagcaa gcaacatgat gatagagttt atgtttttat tatcgttgga
     3901 ttttctgatt tgagaatgtg gttactgtca aaaaaatcag atattaggca ggctataaaa
     3961 ctataaagga gtgattaggt gaaatcacaa aagaatattt ggtggtgggg gtttttagta
     4021 ggaaaggtaa tgataccagc ccttctctta atttctctac cgatgattac ttttttcttt
     4081 accaataaaa aaatgagtaa agaagccgtg atttttttct ttggagacca aaaagctacc
     4141 tttattgaaa cgttagtaac atctcttcag cttaatttga attacatttt ttcaattatt
     4201 attgttatat cgttattgaa tttttttaaa aaaagaaatt caggaaaggt gtttaactct
     4261 aatggaaatg tgtactacaa ctatttttat tttgtatttt gggtagcagc aactttactt
     4321 ggttatgata aaattcagat tgcaggaatt cctatacaca tgcaatataa gttggttttg
     4381 agtggaattt tttctgaggt gttgccagat atatacgatg atcattacga cagtgatgga
     4441 acatgtaaag taagcattga aaaagaaaat ttcgatgaca tcgatggata tgactctgtg
     4501 aacttactga ttattgatac ttacgatata aaaatgagtg aattatctat ggaaaatcaa
     4561 acatatccta cgataattgt tagagggaat tcaatagatg gagttcgtaa agttaataga
     4621 tctttaatct tggaaataaa aaaaacaatg gatgaaattc aaaaaagtga ctttaaaaaa
     4681 gtatttgttg cttcaacatc taatccaaaa aatagtatca atattattaa ttcatcgttt
     4741 agatttttcg gtcgatcacg taggtttaaa ctttatgttc ttcaaaagga ttatgcaagc
     4801 aatggaaagt atagtaagaa atatagaatt tttatttaag aaggaatttt tccttctttt
     4861 tattttggag gaaactatga aaaaaataaa aagctacacc agtatttggg cagttgaaaa
     4921 agtcatttat gcgattaatg actttcagct gccttttcct gtgaccttta accagatggc
     4981 ttggtttgtt ttatccttga tgtttgtcat tgtatttgcc catgtgcctc cgctatccat
     5041 gatagagggg gcttttttaa aatacttggg cattccgata gcggtcactt ggttcatgtc
     5101 gcaaaagacc tttgacggta agaaaccatt gggattttta cgatccttta tcagctatca
     5161 cttacggttt aaggtcactt ttgcaggaaa gaaagttaaa gaacagaaga aacgatggga
     5221 cgaaccaatc accttggtaa ggagtgtgaa ttatgtaccc gattaagtat attgaaaata
     5281 atcttgtctt taaccaagaa ggcgagtgtt ttgcttacta tgaattagtg ccttacaatt
     5341 actccttttt gtcacctgaa cagaagtatc aagtacatga taactttcgc caattaattg
     5401 cgcaaaatcg tgaaggtaag atccatgcct tacaaattgc cacagaaagt agtattcgtg
     5461 ccacacaaga acgttcgaaa aaggaaatta ccggtagact caaagaagta gcaaagcaac
     5521 gaattgattt acagacagat gcattagtat ccatgattgg cgatagtcag attgattatc
     5581 gcttctttat tggctttaaa ctgatttcga cagatgaaga ggtcaatcta aagaatctca
     5641 ggaaatcttt cttttctgga cttcaagaat ttgtctatgg agtgaatcat catttgatgg
     5701 gtgattttgt ttccttatcc aatgaagaaa ttcgtcgcta taccaagctt gaaaaactaa
     5761 tggaaagtaa attggctcga agatttaaag tcaaacgagt aacgccgagt gacttgacgt
     5821 atttaattga acatatctat ggtgaaaaag gcacaccgtt tgaagaatat gaattccagc
     5881 taccaaagaa aaagctgaaa tcagaaacgc tggtgaaacg ttacgactta ttacgtccca
     5941 ctcgttgttt gattgaagaa aagcctcggt acttacggat ggaacatgaa aatcatgagt
     6001 cttatgtagc gtacttaaca attaatacca ttgtagggga aatggagttt ccttcctcgg
     6061 aacttttcta ttaccagcaa caacaattta cgtttccgat tgatacttca atgaatgtgg
     6121 aaattgtgac gaataaaaag gccctagcta ccgttcgtaa taagaaaaag gaattaaaag
     6181 atttagacaa ccatgcctat caatctgata atgaaacgaa ctccaatgta ttagatgctt
     6241 tggattccgt ggatgaattg gaaaccacct tggatcagtc gaaagaatcc atgtataaac
     6301 taagctatgt ggttcgagtg agtgctaaat cagtggatga attaaaacgc cgctgtgatg
     6361 aagtgctcga tttttatgac gataccaatg taaaactagt ccggcccttt ggcgatatga
     6421 tgggtctaca caaagaattc ttaccctcaa gtaagcgata catgaatgac tatattcaat
     6481 atgttacgtc tgactttttg gcgggacttg gttttggggc tactcaaatg ctaggagaat
     6541 tagaaggcat ttactttggc tataacgtag atacggggcg caatgtttac ctgaaaccgg
     6601 cattggcatc acaaggggtc aaaggctcag taaccaatgc tttggcatct gctttccttg
     6661 gttcgttagg tggggggaaa tcttttagca acaatctctt agtctattat gccgtccttt
     6721 ttggtgggca agctgtgatt gtcgatccaa aaggagaaag aggcggttgg aaagaaacgt
     6781 tgccagaaat tgcggatgaa attaatattc ttaacttgac cagtaagcca gaaaatcaag
     6841 gcttacttga tccctatgtg attatgaaga aaaagaaaga ctcagaaagt ttggcgattg
     6901 atatcctgac ctttttaaca gggatttcca gtcgggatgg ggagaagttc cctgtgttac
     6961 gcaaagccat tcgaacggtt acccaaagtg atcagcgagg attattacaa gtaatcgttg
     7021 aattgcgtaa agaggactca ccagtcgcgg aaaatatcgc ggatcacatc gagtccttta
     7081 ccgattatga ttttgcccat ttgcttttct cagatggttc gattacccag tcgattagtt
     7141 tagataagca gttaaatatc attcaagtag cggatttggt tttaccagat gcagaaacca
     7201 cttttgaaga atataccacc atggaattgc tgagtgtttc catgctaatt gttatttcca
     7261 cttttgcctt ggactttatc catagcaacc gagaaatttt caagattgtg gacctggatg
     7321 aggcttggag ttttcttcaa gtagctcaag gaaaaacgtt atcgaataaa ttagtgcgtg
     7381 ctggtcgggc catgaatgcc ggtgtttact tcgtcacgca aaacagtgat gacttgttgg
     7441 atgaacgttt gaagaataat attgggctaa agtttgcatt tcgcagtacc gatattcatg
     7501 aaatcaagaa aaccttggaa ttctttggat tagataaaga agatgaaagt aatcaaaaat
     7561 gcatgcgaga tttggaaaat gggcaatgct tgatgcagga tttgtatggt cgagtaggtg
     7621 tggtccaagt tcatccagtc tttgaagaac ttttccatgc ttttgatacc cgtccgccag
     7681 ttcaagagag aatggagtga ggagatgaaa aagaaacttt ttcgtatctt tggaattgtt
     7741 ttaatcagtt tggtcactct gataatactt ttgagcttag ttggaacggt agctgaggca
     7801 acaggtctgg ttgatgatac agttggagcc ggaaatcttt attcccaata ttcacttggt
     7861 aactatcagt tggatttctt tgtggatagt tcgtgggatt ggctgccttg gaattggggt
     7921 gatggcctag ggaagagcgt aatgtatgga ctttatgcca ttactaattt tatttggaca
     7981 gttagtctct acttatcaaa tgccacgggc tacgtggttc aagaagccta taagctggat
     8041 ttcatttcag atacggctga aagtattggg aagaatatcc aaaccttagc cggcattacc
     8101 gaaaatggac tccagacctc gggcttttac tttgggtttc tattattgat gattttagct
     8161 ctaggagttt atgtggcgta tacaggatta ttaaaacgag aaactaccaa agcagtccgg
     8221 gctgtcatta attttgtgat gatttttcta ctttcgggtt cgttcattgc gtatgcgccc
     8281 acctatatca caaaaatcaa tgattttagc tcagatgtca gcgaagcggc tcttagcctt
     8341 ggaaccgaga ttgtcgtgcc gaattcagaa agccaaggaa aggacagtgt ggatttaatc
     8401 cgagatagct tgttttccat tcaagtccaa caaccgtggt tactcttgca atttgatgat
     8461 tccaatatag aagaagttgg cgaagatcgt gttaacaaaa tcctgtctgt aagtccagat
     8521 gaaaataaag gcaaggatcg ggaagaagcg gtcaaagcgg agattgaaga taatgacaat
     8581 gcgaatctca gtattaccaa gaccatgaac cgcttaggga ccgtggtctt tttggtgttg
     8641 ttcaatattg gcatatcctt ctttgtcttt ctcctaacag ggattatgtt gttctcgcaa
     8701 attctcttta ttatctttgc gatgtttttg ccgattagtt tcttattgtc catgttgccg
     8761 acttatgaga gtttgggcaa gaaggcgatt attcgcttgt tcaatacgat tatgatgcga
     8821 gcaggagtta ctttggtgat tacgactgca tttagcatct ccacgatgtt tttcaatatt
     8881 tcggcaacct atccattttt catggttgct tttctgcaaa ttgtgacctt tgccggtatc
     8941 tatttcaaat tgggcgacat tatgaatatg ttcaatctcc agagtaatga tagccaatca
     9001 atgggaagac gggtcatgcg aaaaccgcaa atgttaatga atcgaacatt gcggcaactc
     9061 aatcgaaatg tgggacggac tttggctttt ggtggtggtg cagcagttgg aaataagttg
     9121 gcaaaggaac aatcaaaatc caaatttaag ccatccggtt cttctctacg aaagaattca
     9181 cgattaccga acgatcacga gataccatct gacttgaata aggaaaagcc aattccaaat
     9241 aataagaaac aatcccgaat gaatttggcc gggagaaaag tcggcaaggt tctggatacc
     9301 caagctttgg tgaaagataa gaccaaacaa gtaaaggatg gagtacggaa tacgccaacc
     9361 aacttgaaat acaacttcca taaaggaatt gaaaaaacga aaaaagcacc ggaagagttt
     9421 aagcgaggac tggtccaaga aaaagcaaat cgaggagagt tgcgagaaaa acaacaacaa
     9481 cgtagagacg aaaaaatagc tgaaaagcgc aaagtattga acgaaatagg gaatcctcat
     9541 gggaaaagaa gggaaaatgt tccgataaaa gcaaaggtcc aatctcaaaa ggatagagca
     9601 ccaaagcgga ttgtgagagc aaattcgaat cccgaaatca aacgaaaatt cgctagccaa
     9661 gaaatcattt caaaaggaat aaaccaatcc tcaagtaaga aaaattcttt tcaacaagtg
     9721 aaacggcggt ctactctgtc caaacgaacc aatcgaaaag tacaaaaaag tttgaaccct
     9781 catcccaaac caaggtcagg tgagaaaaaa tgaacgtaaa gaaattaagt gccatttcgt
     9841 taactttcct aacaatctct tttatgggta tcttgatgtg tgtgggctta ttgtttggtg
     9901 aagacagtga aggaagtagt agttcaggtg ctcctcctgg ggggagtagt gtctcagaag
     9961 aggttttgaa acaccgtgca atggtggaaa aatatgccaa agaatcggat ataacagagt
    10021 atgttccgat tcttttagcg attattcaag tggaatctgg tggcacaatg gaagatgtga
    10081 tgcaaagttc tgagtcagcc ggtttaccac cgaattcatt aagtaccgag gcatccatca
    10141 aacaaggatg tctctatttt gcgtctttgg ttaagcgttc caaagaatta ggttgtgatc
    10201 aagatagtat cattcaggcc tataactttg gcggtggtta tttggactat gtggcaaaaa
    10261 acgggaagaa gcatagtttt gctttagctg aatctttttc aaaggaaaaa tcttgcggtc
    10321 aaaaaatcga ttaccctaat cccatcgcga ataaggaaaa tggtggatgg cgctataact
    10381 atggcaatat gttttattgc ttattggtga agcaatacct aactacaaca cagtttgacg
    10441 ataaaacagt ccaagggatt tttgacgagg cgtttaagta tgaaggaacg gcttatgtat
    10501 ttggtgggtc aagacccgaa acaggatttg attgtagtgg cttgactcaa tggtgttatg
    10561 ccaaagttgg tctgaaactt cctcgggtgg ctcaagatca gtatgatgcc atgacccata
    10621 ttgacctaaa agatgccaaa ccaggggatt tggttttctt tcattcgact tatgatgccg
    10681 gtacttatgt gacccatgtc ggtatttatg caggggagaa tcgcatgttt catgcaggag
    10741 atccagtagg ctggacgaat ctaacagaaa gctattggca gcagcatctt atcggtgctg
    10801 gacggtacaa ataagagaaa agaggaaaaa gaatgaagat aaaaattgaa cggaatcaaa
    10861 aggaaaagag cctaaaaaag aaaaaagaac gagtcgtatc cgtgggtaag catcgaaaaa
    10921 tggtgcttgc cctttggctt ttattatctt gtagtcttac ttttggcgtt tataaaaatt
    10981 tcaccgccat tgatcaacat acaactcatg aaaaagtggt tattaaagag aaagtagtga
    11041 atacttctgg tattgaaaac tttacgaagg atttttcgaa agaatatttt tcgtggaaaa
    11101 ataacaaaga agcaattgaa aagagaatga acaacctcga acagtacctt acagaagaag
    11161 gtttcgcttt aagtcaagat atggttcgag cagatattcc cactaattcc gaagttcagt
    11221 cagtaaaagt cttagatgtt gaaaagagct cggaggaatt tgtcgtttca tttttagtgg
    11281 agcaaaagat tacagaggga aaaaaggcac aatcgatttc ttctgcatac cgagtgacga
    11341 tctttgaaga tgaaaacagg aattatatag taacgagttt gccaactatg attgcaaaac
    11401 ctgatagggc aaaatacaag acaaaacaag tagaaaatga ttcgaaaatt gatgccaaaa
    11461 ctacagaaga aatcgctgag tttttggaaa cgttctttaa attatatcca tcagcttctg
    11521 aaaaagaatt ggagtattat gttgaaaatg atgcgatgcg accaatcaat attgacttaa
    11581 agttttcgaa gttagaggat ccagtttacc tacaaaacgg aaaaaaaatt ctagtgagta
    11641 caaagataaa ataccttgat aatgtatcaa aaaccactag ctactttcag tacaaattaa
    11701 tattacaaaa aaatggaaac tggaaaatca ttgatgttaa ataataatga ctcaatctaa
    11761 atttatgaga agttataagt agtttaactt atttcaggca gaagtcgccc atcctctata
    11821 ggtgggtgat gaatgccgtt cggtaactcg aaaaccgaca attaagacat agtgcctttt
    11881 tattttaatc catgtcataa tcagtctata taagaaatgg ttgatatacc atggaattag
    11941 atacaaataa tcattcatta tttcttcttt gttaccacct tattttagta gtgaaacatt
    12001 gtaaacatct cattgatgat gtaatgttag cttatgcaaa aaactatctt tgaaagaata
    12061 tcagaatcac atcacattac cttagttgag tgaaatcgtg ataaagacta ctttcatatt
    12121 atgctcaaag cacaacctaa aaccgaatta acaaaattca gcaatgccta agctcatgtc
    12181 tgataaaatg agactttcta agggtcaaaa atttcctttg aatacgatca ttagcttaga
    12241 taaaagtaaa aaccttagaa ctacggggat agcttggtta ataagagata tcgctgttaa
    12301 taaagacata agcttgcaag tatgctctat tcccaagaat ctcccacttc aagcactaag
    12361 gatcgttaaa ttcagctaag tggcaagtag ttcactagac gataattatc tccatatttc
    12421 aatctcagct tttccatttt tcatagcaaa ttccgttatt tttatagcat ctaaaagatt
    12481 cagcttttcc actgatcttt caccactttt cagctttttt attgtgctta aattgatacc
    12541 agtacctttt gatattgaat tggaagtaaa atcattaagg gtttttttta ttaaattcat
    12601 atccgcaatt ccagcgttca taagaatgaa gattctcctc tcatagacta ctttgaaaat
    12661 ggtactacag tggctgttta tatatttcag taggttactg tagtggtcat tttattataa
    12721 ttagaaaagg aacctaaatc aaaaatgcta aagaaaattt tcatagtatt aaacctatca
    12781 tgactaaagc cacggaattc ttgataaaag agtatacttg caagcctatg tctttactaa
    12841 cagcgatatc tcttattaac caatctatcc ccgttgttcc taaggttctt gatgttttct
    12901 aaggcaatgc ttgtatacat agcccttgat caaaatattg atactaacgt tgacgtttcg
    12961 atcatgatgt gtacggcaaa taggacaagt ccattctcga attgtgaatg acttcttacc
    13021 atctgtgtgt ccacattctg aaccaatttg actagacgga aatcatttat ctatcttgat
    13081 gatttttttg acagaagtat cgaaaatctt tacataatct gggtgttgca ttcttatgca
    13141 gatgagagcg ttagctccgt attcataacc agttgcctct aaaacgtagt ccatcggtgg
    13201 atacctagct tgcggttgcc agtggctttc gcagtcttct tgtaatcaaa tcgagcatct
    13261 aattgagtac tagtattttg aagataatac ttgagttttt cggttttcag aagttcactt
    13321 catcttccat catccatgga aatcccactc acccatacgc aggcctccaa aaattcttca
    13381 acaatggttt ttcagacatt tttctgaata cctccttgtt ttttgatacg ccagatagtc
    13441 aggaaaaagg caacaaaaca caaaacaaag ctgcctaaaa aggtaatctt cattccatat
    13501 aaaaagacat ccggccgatc tgcgatatag ttagttactc gttggcccat cttagcagac
    13561 atcgcttgat ataagaccgt cgtagaaagc gaaatcccga tgaccatccc caaatttcgg
    13621 gcaaaggaat tcatggagcc tgccagtccc aaattttcga tagtgacaga actcataacc
    13681 atcgtattat ttggggactg gaacaaggca ttgccggcac ccatgatggc agtagcgaca
    13741 atgtacagcc acaaaggaga gtccactgac aaaaacatat acatgacttg ggttaccgac
    13801 aataaaccca agcctactaa tacaaaaatc tccgggccaa cccggtcggt caggtatccg
    13861 cttaaaggcg ccccgacgac catcatcaag ggaaagacca tcatcaacag tccggcatag
    13921 ctaggactca acccgcgagc attttgcaga taaaaaggaa tcactacatt aacaaagaag
    13981 ttggaagaaa aaatcaacaa ggctgtaatc aagctcatgg taaaaatctt gttcttaaaa
    14041 ataccgaaag aaatcaatgg agaggctgct ttcttttcaa cttttaaaaa gctgacaaag
    14101 gcaatcactg aaattacgaa cagtacaagg gggactggat ggaaaaagcc actttcttgc
    14161 cccaaaaaga ttgttccaaa aaggctgata atcgtagccg caatggcagc aaagccccct
    14221 ccatcgattg tatggccgct catggtgata tcttttggta agaatttttc agcaatcaac
    14281 atcgttacga ttcccaccgg tacattgatc caaaaaatgt acgcccaaga aaattgggac
    14341 aaaatcaagc cgccaagacc tggtccagca atcgagccta atgaaacaag cgcaccgatt
    14401 gctcccaatg cccgaccacg ttcatgtatg ggaaagactt ccgtgatgat tccggcattt
    14461 gtagccatag tcatactagc accaatggct tgtaccaccc gtgcgaacaa ttaaatacct
    14521 agcgaatgat tgaacccaca caacagcgag cccaagacaa agatcagcgt accaatccga
    14581 aatactttga ttttcccaca gctatcgcca attttaccaa acagcaacaa acaagaacag
    14641 acaatcatca gataaacgga cactacccat tccgcttgat tcattggtat gcccatatca
    14701 tctgagatgg taggtagtgc aatattgaca atactgccat ccaatgtgga catgaaggta
    14761 aacatagata tagcaatcaa aatccaccag cgattttttt ggacttttgc atcttcttga
    14821 taactatgca taataggatt tcctttctaa tgatcgtgta gctaagaaca atataaccta
    14881 tgtctactgc ccaacaactt ctcgcgactc tagccatagg aagcttgagt aatttcgatt
    14941 tgttattctc atgtaaccat gtaagtttga gatgctttat ttgaacttac tctatagtaa
    15001 ctagtgataa ttcgctttac tttcttctag caatttctaa aagtcgactt cattgttctt
    15061 cagattaata gtgtgagcta tctcgtgaga cgtaagaaaa tttccattct actttttccc
    15121 aattttttca tatttatggt gttgcaccta tttgttttcc aatttgcttt tcaaatacat
    15181 tgctatgata ttcaattcca taggattata ctaaatgtca atagtatgaa taatatatta
    15241 tagatgttta tatatcactg ttaattatta tccacaataa ttaatgatta cctcgatatt
    15301 tgtcaatcaa aggttgaaac aatttatttt cagcatcttt tctaacttga gctgcactct
    15361 taatagaatc atacgatcct aaatagtacc tctttttttt aaagttatat acgcaatcca
    15421 tttctcttta gaacgataaa ctcccttaac accacttgta ttatttttcg gtaattttga
    15481 cgttagacac tctattactg tcccttcaac acatgtcctt gtttcttgcc tttttaaaca
    15541 tccacaactt tgagtatgtc caaaaaataa gttactataa cttatctcac acatactgcc
    15601 acactcacat tgacaaaccc attttgtttt ccatgaacag ttattgtcca aaggatactt
    15661 tgcagtaagt aatccgaatt ttttattttc aatgtgatgc ttttttgatt tcaaacatcc
    15721 acagcttttt acatcacctt ttagaagtcg ctttgccgat actaactttt ttctcccaca
    15781 cagacattga cattcccaaa caacagttcc ttcacttcgc agtttagttg gcttgattgc
    15841 aactaactta ccaaaagttt tatttgtcaa atttttatat ttatgcctat ttttataatt
    15901 tccacatcca caacttttgg ttctacctga tagtaagttt gatcgatata caatcgtttc
    15961 attcccacag tcacataggt aacgatattt tttatatcca ttatgagtaa tctcctcaac
    16021 aacagtaagg tgaccatatc tttccttcac tgcacctacc ctcccaagaa agaagtacca
    16081 tataatttca attaactttt aataaatcag aaaaaaatat tttcaatttt tcttagtctg
    16141 attttatgac taatttataa tacaagactg aacgatacgt acacgaaatc ctcaagatag
    16201 ataaattgtt ttcatctagt aaaacctgct caaaatatga gcataaagac ggcaataata
    16261 cactcgatat tcgataatgg acttgtttta tttgtcaaac tcatcgtaat cgagatatca
    16321 attccagtct caatatcctg atcaaaggtc taagaatcca accattttct tgttactttg
    16381 tcagattgat tacctttttt agtctccgaa taataaggtt ttcggatcaa ctaacatgct
    16441 ctgagaaaga ctactatctt tgcaaatttc caaatgaagg tgtgggccag tactattccc
    16501 agtacttcct acatagccaa ttatttgtcc ttgcaaaaca ttatctccag tttttactag
    16561 gtattgttgc tggtgagcat ataacgcggt tgatccatct tcgtgttgta tcaccacgta
    16621 atttccccaa gagtagtgaa actctgcttt aacaacctta ccagctttaa ctgcatgaat
    16681 cggctcaccc tgtgtagctg ccatatcaag tcctcggtga aattcatttc cacgatttcc
    16741 aaacaaactg gttattgaat aattatgaat aggcacttgg tatccagtct tactcatttg
    16801 tggaccttta acttctttat catagttggt taaatcatag gttttaatta attcatttag
    16861 cttcaaatag tattgtgtat ctgttgcata ccgtcctgta aggaatttag ttgcttctcg
    16921 atatgtttta ctattagctt ttaatgctcc tgcataaaaa tctttatttc cactaattcc
    16981 cttagttaat aatttggcat aatcttcaaa actttcctca tagttttcat acactctaaa
    17041 ttttgattgg gtcgtataaa gcgttccatt acccaaatct tcgtttgtta ccataatgac
    17101 aaagtttcca ttataagttc cttttatccc aaataaattg taattcggtg cctgagctaa
    17161 ctggctttga ccactggcag attctaagat cgcttgtgca atcataacag aagcatataa
    17221 atcatatttc tgaccaattt ttctcgcaga ctcaccaatt ttcataataa aattttctac
    17281 agattcattt ttgtcaaaat gaatttcttc attaccagat agtattggtt gtgattggaa
    17341 agattctgtt gattctattt gctgtggtgt cgaaaaattt tttgtttgtc cttccgtttt
    17401 tggcgcttcc ttttttgaag actctatatg gtttggtctt gtcactttcg aagaagttat
    17461 ttcacttgta ctacttgaag ttgttgaaga ttcggcactt gaaatcgtac tactttcact
    17521 tgacgaagtt gttgaagact cggcatttga agtcgtacta ctttcacttg acgaagttgt
    17581 tgaagactcg gcatttgaag tcgtactact ttcacttgat gaagttgttg aagactcagc
    17641 atttgaagtc gtactacttt cacttgataa agttgtcaaa agttcactac tctctgctgt
    17701 tacatcggaa ttactcattt cagtcgtctc ggttctttct tttgcaattt tgtatgttga
    17761 attttcgctt gccaacacat ggaaaggtgt tgctataaag agttgggaca ttaatataac
    17821 tgctgatgca agttttaaag attttttcat ttgatttacc tctctttttt ctctttaata
    17881 gtgttttcta ttaaaaatta tgataaatat tttcaatgaa ttgagtaata cagtatattt
    17941 cttgatttca tttagtaacg attatgtttc aatgtttaat cctgttactg cctttaaaat
    18001 aaaattatag ggttggctaa aactaggatg aaataaataa tctaacattt tcaatttgtc
    18061 taatgtaatt tgttcttcta tagcaagaga aaacattgat aatagtccag ataaatcttc
    18121 ttgactacaa agttgtacac ctactattct ctttgtattt ttttcaaagc taattcttaa
    18181 ttttacacga ctattttttc tcaaaaaaac aggtctaatg aaatcttcta tttctatata
    18241 atctatagac tcacttaata atcgtgctgt ttttaaattc aaacctgctg cgtaaaaatt
    18301 gtaattaaat atagaaaagc catttgtaaa ttgggaacca gaaggtgtaa aatcttgatt
    18361 gtcaatatgt tgagttgcaa tataagctgt tcttagagca ttagaaatag aaaaatcaac
    18421 actacttcta ttaatggacg taaaaaaagt tgtagcacaa tccccaatag catatatatc
    18481 ttttatattt gtttgctggt attcattagt tagatatgct ccactaacat ctctcaagag
    18541 ttcctttttg ccaattggac tattagatag aaatcctata gcttgtacga ccatatccat
    18601 agagtattgg gtaccacttg tagtgactat tttttcaatc ttttttcttc ctaaatattt
    18661 tttaggactt tctgatagac aaagatgtat tccatttttt gatagtattt cttcaacttt
    18721 atctgaaaaa ctacggtcgt aataagtcga caacactcta tctgcaacat cgaaaagata
    18781 tacttctttc cctctttttc ttatggcttc agcaatttca actccaatat atcctgctcc
    18841 aataattcct acttttttaa tagtattatc atctaaagct ttaaaaatct ctttagcatt
    18901 tggaaaattc tttatcgaaa aaatattagc taaattattc cccggtacat ctaacataat
    18961 ttgctttgaa cctgtagcta gtataagttt gtcatattct tgggaaaatc ttgtgccatc
    19021 tttttttaca caaataatct ttttattttg aatatcaatt ttgtcaactg ctgtctccaa
    19081 aaacaacttt tttatcttag aatttagttc tcttctattt tgataaaaaa gatcttctaa
    19141 attgctaact tctcttttta tgaacaacga tgtcccacat tctaagtaac taatttctga
    19201 ttttttatct atcagaataa cttcataatt tgaatcacat aagtttagaa atcgagctgc
    19261 agctagcccc gaatggtttg atccaataat tactattttt ttcacttaat cacattcttt
    19321 tcccttctat gaaaagtatc ttcattttta actaacaacc tatctcccaa aataaaaaac
    19381 aaaaagagga agaaaaagat tcttcctctt ttcagagaag atttaagaaa ttgattttct
    19441 attcattctc tttttttcga tttttatacc ctaataaccc taaagttcct aacaggatta
    19501 aaccaccact aatagtaaag atattactat caatctctcc cgtcttagga taagcttttg
    19561 aggactgctt ttgaggactg cttttgcctc tatttttgtt ttgcttgcta ccttgattgt
    19621 cagtcttatt atctttacta acggcttttt cactgtcttt ttcgtttgaa gaaacaacta
    19681 cattagtagg ttcactagta ttaccagatt catcagttgc agttactatc acttttgttc
    19741 ctgcctctaa tttaggaact ttaactgtaa aattaccatt tttatcagct gtcccatggt
    19801 attgactccc gtttggtaat gtaactgtca cctctgcatt tggttcagtt ttaccagtaa
    19861 caatttggtc accctctgta ataggatcaa cttgaggttt attcggtgct tttttatctt
    19921 ctacagaaat agtaacctct acttcttctt tagatccatc tggatatgtt actactacaa
    19981 tccctttttt gtttccagct gtactaacat ctactttctc tttccaagta taagtagttc
    20041 cttttggtag atcttctttg ttcttaattc cctcagatgc gtctggctct ttatttaagt
    20101 ctgtagtcac tttttgtcct tcaggattgt atttatcggc atcggaacga ttgtctgtca
    20161 cttctactgg aactttcact gtatctttcg taccatctgg atacgttact tcgataa
//
LOCUS       DANKKG010000043        19509 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000043 DANKKG010000000
VERSION     DANKKG010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.043"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(460..1089)
                     /gene="udk"
                     /locus_tag="PW004_001294"
     CDS             complement(460..1089)
                     /gene="udk"
                     /locus_tag="PW004_001294"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HDL2726277.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1173..1868)
                     /locus_tag="PW004_001295"
     CDS             complement(1173..1868)
                     /locus_tag="PW004_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HDL2726278.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1881..2597)
                     /locus_tag="PW004_001296"
     CDS             complement(1881..2597)
                     /locus_tag="PW004_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HDL2726279.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2927..3532
                     /locus_tag="PW004_001297"
     CDS             2927..3532
                     /locus_tag="PW004_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HDL2726280.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            3857..4180
                     /locus_tag="PW004_001298"
     CDS             3857..4180
                     /locus_tag="PW004_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HDL2726281.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4362..5732
                     /locus_tag="PW004_001299"
     CDS             4362..5732
                     /locus_tag="PW004_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDL2726282.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5862..7163)
                     /locus_tag="PW004_001300"
     CDS             complement(5862..7163)
                     /locus_tag="PW004_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDL2726283.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(7393..9351)
                     /locus_tag="PW004_001301"
     CDS             complement(7393..9351)
                     /locus_tag="PW004_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HDL2726284.1"
                     /translation="MAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9528..9794)
                     /locus_tag="PW004_001302"
     CDS             complement(9528..9794)
                     /locus_tag="PW004_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDL2726285.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10304..12430)
                     /gene="pnp"
                     /locus_tag="PW004_001303"
     CDS             complement(10304..12430)
                     /gene="pnp"
                     /locus_tag="PW004_001303"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HDL2726286.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12618..12887)
                     /gene="rpsO"
                     /locus_tag="PW004_001304"
     CDS             complement(12618..12887)
                     /gene="rpsO"
                     /locus_tag="PW004_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HDL2726287.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13138..13701
                     /locus_tag="PW004_001305"
     CDS             13138..13701
                     /locus_tag="PW004_001305"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDL2726288.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(13954..14475)
                     /locus_tag="PW004_001306"
     CDS             complement(13954..14475)
                     /locus_tag="PW004_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HDL2726289.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14478..15320)
                     /locus_tag="PW004_001307"
     CDS             complement(14478..15320)
                     /locus_tag="PW004_001307"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HDL2726290.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15313..16038)
                     /locus_tag="PW004_001308"
     CDS             complement(15313..16038)
                     /locus_tag="PW004_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HDL2726291.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16214..16510
                     /locus_tag="PW004_001309"
     CDS             16214..16510
                     /locus_tag="PW004_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726292.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17078..18670
                     /locus_tag="PW004_001310"
     CDS             17078..18670
                     /locus_tag="PW004_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL2726293.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18683..19423
                     /locus_tag="PW004_001311"
     CDS             18683..19423
                     /locus_tag="PW004_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL2726294.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
BASE COUNT         5582 a         4002 c         3702 g         6223 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaaa cagttggctg gtgactgttc aaaaagcaga atccttgctt
      421 ttcaaacggt cgcagtccgt tcaactgttt cgctgcgcac tatttatggt ttaagaaact
      481 agctactttt gttgtaatca aatcaatagc aacgtggttt tctccgcctt ctggaacaat
      541 gatatccgca tagcgcttcg ttggttcgat aaattgatga tacatcggtt taacaactgt
      601 caaatattgt tcgatcacgg agtccaatgt gcgtccccgt tcttccatat cccgtttgat
      661 acgacgaatg atacgaatgt catcatctgt atctacgtat actttgatat ccatcaagtc
      721 acgcagacgt tcgtcttcta aaatcaggat tccttctaga atgatcactt cttttggttc
      781 ctgaatgatc gttgcctgac ttcttgtatg cgctacatag tcatacacgg gtttttcaat
      841 ggctttatag ttcagtaagt cgcctacatg ctgaatcagc agatcattat caaaggcaaa
      901 tgggtgatcg taatttgtgt tcaatcgttc ttcaaaactc aaatggctct gatccttgta
      961 ataagaatct tgttcaagca tcatgatgga atggtccggg aagttgttga aaatcgcacg
     1021 gctgacactt gtctttccgc ttccggatcc acccgtcaca ccaatgataa ttggcttgct
     1081 ttttgtcatt gactaaaacc tctttcgtat ccgttttttc attctaatct attatagcga
     1141 aaaaacacga aaatgctata tttttctgaa aattaatcct tttcaatttc caataatgaa
     1201 taaagttcct ctaatgaatc gattcgataa gtaggctgga tttcttcgat ttctggtaat
     1261 ttatttctat tgaaccaaat cgtatcgatc cctgccatat ttcctccttt gatatcagaa
     1321 gtcaaagaat caccaatgat cacagtttgc tttttatcaa aattaggaat gtgggaaaag
     1381 acatgatcaa aaaattcttt catcggcttt tgcgctccga cagcttctga gacaaagata
     1441 tccttgaagt aatcaagcat tcctgcatct gttaatcgcc gatgttgcgt gtgcgacaca
     1501 ccgttcgtca caatataaag atcagcatgc ggatggacct ttttcaggat ttctagactt
     1561 cctggaacaa gatcatgacc ttggttcaag tagtggcggt agcgtttttc catttgatca
     1621 ctatctactg ttttcccaaa ttgctggaaa agtaacccaa aacgactgcc tattatttct
     1681 tctcgtgata gtctgccttt ttcaaactcc tgccataaat gatgattcaa agccttatag
     1741 ctggtttcga tttctggtgt caatgtgatt ccttgttctg caaacaaagc acttaaagca
     1801 ttggcttcag ttaattgaaa atctaacaat gtatcatcta catcgaacaa taaagtttta
     1861 tatttcatct ttttgacctc ctatttaaaa taaatactta actttttcat ctctgctaat
     1921 gtcaacgatc gatactcgcc tactcctagc tgttcatcta attccaatgg tcccatgctt
     1981 agccgtttca atgcggtcac tttctttcct tgggctaaga acatcttttt gacttgatgg
     2041 aattttcctt cttggatcgt caataaaacc gtactttggt gcttgtctgc tttgagtatt
     2101 tttaaatcag ccggcttaca ttgataccca tcagcgaata cgattccatt ttcaaatgct
     2161 gttacgtctg ccggtgtcac cctttcgttc acaactactt gataagtctt tgaaaccttt
     2221 ttttctggaa gtagcatttc atatcccaat tgtccattcg aagtgatcag caatagtcct
     2281 gttgtatctc tatctagtct tcccactgga tataatggtt ggaaacgatc ggcacttgca
     2341 atcagatcga ttactgtcgt cttttctgga tctgatacag ctgtaacagc tttttcaggt
     2401 ttattgagaa gataatagga ctcatttgtt tctaagtgtt cacctgctac ttcgatttga
     2461 tgcagtaaac tatctacatt tcgattttgt cgaaattcga ctgtgccatc tatcatcact
     2521 tgcttcaaag caaacagccg cttcatttcc ttgcgtgtcg tttctaattt ttcttctatt
     2581 aatttatcta aacgcatgct gtttcctttc ttattactac taattaatta taccttgaat
     2641 attcttgctt gcaaatacat catcatagtt attccttgac ttaattaaca gttcacgtta
     2701 cggttaaata gtaaagtgaa ttggatttct catacacagc gtaatgacct ggtggagaaa
     2761 agttgaatcc tgactttcta aggggagatg cgcgcatctc tccttttgcg cttttttaag
     2821 cgccctgcct tcgatgatgt gcacgagatt agcctttaga attcacttaa ataaaatctg
     2881 tccgttacca aagcagacag tcacatttta aggaggaatt ttggttatgt cagtatcatt
     2941 agaagtaagt aaaagagaag tacgtccccg ttcattaaga aacaaacttc gtcacgaagg
     3001 gaaagttcca gctgttgttt atgggtataa agtagaaagt acccctatct cattcgatgg
     3061 tttgacacta agcaaattgt tgcgtgaaca tggagcgaac acagtcatca cgatggacat
     3121 cgatggacaa aaagtcaata cattggttca caagatccaa acgaacactt ttaccaatca
     3181 tttcgaacat gcagaatttc tatctgtaaa catgtcggaa gaaacagaag tcgaaacaga
     3241 agtcgttctt gttggcgaat cagtgggtgc taaatctggt ggagtgttag ctcaaaactt
     3301 gtatacagta gtcgtatctg ctacaccaga caaattgcct gaacgaattg aagttgacgt
     3361 gacaaacttg gaattaggtg attctattac agtagccgat cttccaaaaa acgacgatta
     3421 caaattcgtg accgacagtg aagagcaaat cgctgctgta gtcgctcaaa gtgaagaaga
     3481 aagcacaggt gaaggtggcg aaccagaagt gatcggttca acagaagaat aatactgaaa
     3541 caatcaggtg gtgttagaag cggcgggtca gcagctttta acacgccttt tttatttcta
     3601 atcgtttatt ttcatatggg gcttccactt ttgtaaatgg ctttacaaac gaaaaatcct
     3661 aatttaccaa gcttttcctc gcatactttt atcgtacctt tataaaaatt tatactttga
     3721 aaaaaagtat tcaattattg ttgcattttt cctgattctt tattagaatg aaccctcgtt
     3781 gaagtaagta tttctagttt atcctaggat taattataaa aataaaacat tcgttgattc
     3841 gaaggaggtt aaacagatgt ttgaaagttt tgatagcagc cgcaaccgtt acgcttcagt
     3901 tggtgtcgta tcaagtttgc cagatgaatt gattgacagt atatggttta ttattgacct
     3961 agatcttaag ggagtcattc cattagaaaa tatattaacc tttgatttga tcgaaaatcg
     4021 tggaaaagtg acgatgcatt tttctcaagc aggaagcaat gtggaaatgg atattgattt
     4081 gccgtttgcc tattcgaacc aatttcctgc tcaagtatac gcctatgatg acggtacgag
     4141 agaaacgatc cttttaccaa gtgagattag acaatattaa ggtgatagat aaacgtggat
     4201 gtcccttatt ttacagacca ggccaagttg ttgattcttt gactcaattc ttggcttctt
     4261 tttatggagt ttcataaatt atcattgatt tctaattggt attctcagga taaacgctat
     4321 atactactaa ctaaatttta aagatataag gagtttaaaa gatgcgcgat ttaacaaaag
     4381 gaagccccgc taaactgatt ttgatgttca ctgttccttt actgatcgga aatgtttttc
     4441 agcagttcta caatatgatc gatatgatca tcgtgggtca gacgctaggt aaaaatgcgc
     4501 tggcagcggt tggtgcaact ggaagcctga catttttgat cattggcttt gcccaaggtt
     4561 taacagctgg tttagccatt attaccgcac aacgttacgg tgcaaaagat tatcggggtc
     4621 tgaagaaaag ttttgctgct agtgttgtca ttagtcttgt cgtcactgtg attttaactg
     4681 tcctaagtct cctgtttatt cgtccgatgc ttcaattgat gcaaactcct cctgaaatac
     4741 ttgatcaagc acaaacattt atttcaatta ttttattagg gatctttgca tcagtcagtt
     4801 tcaatttgct ttctaatgtc attcgcgcac ttggtgacag ccgcacacca ctattctttc
     4861 tgatcattgc ggtcatcatc aacgtcgtac ttgatttgat ctttattatc tattttggca
     4921 tgggtgtgga aggtgcagct attgctacag taatcgcaca agtttcttcc agcgtactgt
     4981 gtctagtgta tatcaagaaa aaaatgcctt tgttacagtt acgcaaaaaa gatttcaaat
     5041 ttgaaaaaga agagtttgct gtacacctaa atgctgcttt gccaatggcg tttcaatctt
     5101 cgatcatcgc tgttggtgct atcgtgttac aagcagcact aaatagtcta gggacagatg
     5161 tcgtagctgc tcaagccgct gctggtcgta tcgaccaatt tgcaaatcag ccgatgatgt
     5221 catttggtat tgcaatggca actttcactg cacaaaacta tggtgcgaaa gaatatggcc
     5281 gaatcttgca aggggtacgt caaactttac tgatgagtgt cggatttagt ctcttagcag
     5341 gtgctatcgt cattttcttc ggtcatttct ttgttcaatt gtttgttagc ccatcagaaa
     5401 cacgtgtatt tgaattagca caaacctact tcaatatcaa tgggtctctt tactggatct
     5461 tagcagtcct gttcatttta cgctacacac ttcaagggct tggacaaagc aagatcccta
     5521 cgatcgccgg tatgatggaa ttgcttatgc gttcttttgc agctatcgtt cttacaggaa
     5581 tgtggggata cccaggagca gcagcagctt ctcctttagc ttggatcggt tctgttgccg
     5641 tgttgcttta ctcttatatg cgttcgatga aacaattgaa acacatgggt gaagaggaac
     5701 atttcacatt agaacatgca gaagcaagat agccttgatt aaaagaaatc gttgcggcta
     5761 aaaatgaaaa cttttattga aaatatcttc ttcgtttacc gaaaacaggt tttttagata
     5821 acaaaaaggc tgtgacaaaa aatgtcacag ccttttttct tttattctat ttttctttcc
     5881 gtggactcta ctttgaaata atcgatatgt gcatgctggt attctaatac cgtgaaacgg
     5941 aatgatgcaa tctcaatgat tgttcctgct tttatttctt tatatcggct agaagcaaag
     6001 cccgcaagtg tagtaaaatt cgtttcttca aactctggaa tctctacatg aaaataacgc
     6061 tggaaatcat ctaatgttag tttgccggag atgatataag agccatctga ctgttccgct
     6121 atcaatgctt cgtccaaatc tgtctcatct cggatttccc cgacgatttc ttctaaaata
     6181 tcttcaatcg tcactaagcc gcttgtccct ccgtattcat cttttaagat agcaatatgc
     6241 tttccctttt ttcgcatgat cgtcaataat ttttttatag ggatcgtttc agttgttacg
     6301 atcggctctt caatcaactg ttctactgtc agctggtcat ctttataaga ttgcttgatc
     6361 aaatctggca aggtgacata acctaaaaca ttgtcttttg attctaagat aaccggatag
     6421 cgagtatgtc cttgttcaat cgaagctttt atcgcttctt tgacggtttc tgaagcttcg
     6481 aatacttgca tggacgtacg gtctacttgt atatcttttg ccaaggtatc atcaaattca
     6541 aacacgtttt ccaaataatg atattcttct ttggtcaact ctcctttttc ataagaattc
     6601 aatgcgacta atgtcagctc ttcttgagac aatgtctctt ctccttcaga tacgagacgc
     6661 acacctaata gcttccctag ccctgcagct gaatgattca atacccaaat aaaaggaaac
     6721 attactttat aaaaataatg aagtggttta actacgaaca agacgacctt ctccgtattg
     6781 acgatactat agctcttagg aagcagttcg ccaataacca catcgatata cgtaactaaa
     6841 ataaatccca gcgccacaga gattgtactg ctaatagccg aactaaaagg tagtttaccg
     6901 atcactggtt ctagtaaagc cgcgacagat ccttcaccga cccacccgat gatcaaaccg
     6961 gttagcgtaa ttcctaattg actagctgat aaatagttat ctaaatggtg tgtaacatga
     7021 agagctagct tggcgttctt tacattttcc ttttctagtt gttccaatct tgatcgtctg
     7081 attttcacta aagcaaattc acttgctaca aaaaatgaag taattgcaat catcaaaaat
     7141 aaaataatga aattgataac catattctcc cgcctttctc tttttctatt gtaccctttt
     7201 tctacaaaaa gattgaaaat aatgcgtatt catatttagg aataaaaaag gagatgcggc
     7261 atttttcagc cacatctcct tatcacttca aaatcattgc gttacttgtt gctaaacagc
     7321 tttttcatga cctctgataa acggtgttca agtagctgac attcgacatt agttcaggtc
     7381 aaagcgctac tttcacaacc gctctttcaa ccatgttttc cgagtatttc caatgataag
     7441 cggaacagtt tcatgtttta cttcgctgta ctctagccca aaccatgttt ctggcgaggt
     7501 agcgattttt ttgattgcca gttttgcctg catgatttga cgttgccatt ttcctagagt
     7561 tgtcacattg gaaactactt catcaatcag gacaaattgg aaatcgccaa cttctcgccc
     7621 tggtgtcaga gagtatcttt gtggctgttt tggcaaacgg ccttgtttca tcaaatcatg
     7681 gatgacttgg cgaacatata cgttgatttc ctgttggacc ctgaaaccta agaacagatt
     7741 cacttggaca ataaaatcag tgtccatcat atctacttga tattctttcg tatatggctc
     7801 atccgtcact tccacatgaa caaaccaata gactcgtgct cttttaggcc gtttatctaa
     7861 aatcgaatag atgaactgtc ggccaacttt atcttcagcc atatcaggaa ccaagaagac
     7921 cacatttgtc tgatagattg gtaaagaagt atcatcacgt aattctctaa gctgcgggat
     7981 ataatctctc aaagacacat cctcggttga cgattcgcga ataatgttcc cttgttccca
     8041 aataaacatc acgactaaga tgatcaaacc aatcagaacg gctacaaaac cgcctcggaa
     8101 gaatttgaca acacttgaga taaagaaaac gacttctatc gcaccgaaga aaaaggtaac
     8161 taaatatgca agccaacgag aatgcccatt ttggataaga taaaagtaaa gcagtgctgt
     8221 tgtcatcaac atcgtcacag tgatcgatag accgtaagca gcttccatat gatgagacgt
     8281 tcggaatgtg acaaccacta gagaacaggc gatccataaa atcagattga ctgctgggat
     8341 atacatttgc ccgatactgt tcccaggata catgatcttt aaccgaggca gcagtttcaa
     8401 cttgattgct tctgaaacta gcgtaaacga acctgtaatc aatgcttgcg aagcaatgac
     8461 tgcagcgact gttgcaaaag caacgccgat cacgatccaa ctttgaggca tcatttggaa
     8521 gaatggattc aggttcttga tttgctgtgc ttcagggttt tgataaacat tcaacaacca
     8581 cgcagcctgc ccaaaataat tgataaccaa acaaattttg acgtaaggcc agctcagacg
     8641 aatatttcgt ttgccgacat gtccaagatc ggaataaagc gcctctgcac ctgtcgtagc
     8701 caggaaaata tttccaagaa tgaacaagcc taatttgtta tctgcactaa aaagcagctg
     8761 gatagcataa taaggattca gtgcacggat aactgtccag tcttgactga agttcattaa
     8821 tcccattacg cctaaaaaag taaaccaagc gaacatgatt ggtccgaatg cttttccgac
     8881 aatatctgta ccaaagcgtt ggaccatgaa taaaacaaaa ataataacta aagtgataat
     8941 gacgatcacg ttctggttat taccgaaccg gtcaaaaaac tgcggaattc ctcgcaagcc
     9001 ttcaatcgca gtcgttaccg tcacagcagg ggtcaatacc ccatctgcta gtaaggcagc
     9061 tcctccgatc attgcaggaa tgatcaaata tttcccacct tttcggacaa gtgtgtagag
     9121 tgagaaaatt ccaccttcac catggttatc tgcattcaat gcaatcacga catatttgat
     9181 tgttgtcagt agagtcaatg tccaaaaaat caatgagact gatccgataa caaaatcagg
     9241 attgattcct gacaatccgc cattatcttc aacaatcgat ttcataacgt aaagcggact
     9301 tgtcccgatg tctccgtaga cgactcccat cgctacaagc aggccagcca ttgatatctt
     9361 tttggtattg tgcgttgttg ccacgttttt ccctcatttc ttgttatatg gatatccttt
     9421 aggtaagctt gaatgataga tgatattctt atccatgaca cccgctttcc taaattgctt
     9481 ctaattatcc aagtaacaca ctgaagtgtc aacttatttg aataaaatta ttctttcttt
     9541 ggacgttctc tgatacgtct tgccacaaat gaataaagag caaggcaaaa aattgcaccc
     9601 aataatgctg gtatcaaata aaaaccgccg agttccggtc ccagtggtcc tagtaaaaat
     9661 tcgccgagcc aactacctaa aaaaccgata atgatatttc cagatacgcc tcccgggact
     9721 tcctttccta aaattgcgcc agctatcaaa cctaatatac cgccgataaa taacgaccat
     9781 aaaaaattca gcatctcctt tccctccttt atccatattt ctcactttta ttttacgctt
     9841 tattcagaag gactccaaaa atatgcgcca agaaactcca gaaaaaaaga agttgtaaca
     9901 aaagtgttca gcttatttcc gtagaagctg cttctggaac accgtgaatc agaggttgtg
     9961 aaatcagcgg tttgacctga gtattagaga aacaaacgga aaaatagttc ctcatatttt
    10021 gccgtttttt gagctaatat cgagggacag ctgattgaac atcgtttatt ggagaggttg
    10081 taaaaaagct gttctgcatc aagtaataag aacagccaag taaaaacagc tcctaatgtt
    10141 tttaacttgg ctggacttat taccgcagat gcaagctttt gaacaccgtt tattcgaaaa
    10201 aaaaggactg tgacacggct tttgtcacag tccccttttt tcgaataatt attgtccccc
    10261 tgtcaccttc acgatcacaa gtacattatt cctatgaatt aaattatttt tcttcatttt
    10321 tcgttgaacc atcttcgttc aacattgctt ttcttgacag atttacgcgt ccttgtttat
    10381 cgatttcagt tactttcaca aggatctcat cacctagttt aacaacatct tcgactttat
    10441 tgacacgatc gtttgctaat tgggaaatat ggacaagtcc atcttttcct ttgatcaagt
    10501 tgacgaatgc accgaatttt tcgatacgga caactttccc tagataaact tcaccaactt
    10561 tgacttcttt ggtcaaatct tcgatgatct tgatagcttt tttgatcatt tctgcatcag
    10621 ctgaagcaat acttactttt ccatcttgat cgatatcgat tttcacacca gtttcatcga
    10681 tgattccgtt gatcgtatcg ccgcctttac cgataacatc tttgatttta gcaggttcga
    10741 tctggatcat ttcgattttt ggtgcatatt ggcttaattc ttcacgtgga gcagcaatcg
    10801 ttgaagtcaa ttcttctaag atttccatac gtgcttgttt tgcttgagtt aatgcttcag
    10861 ttaggatttg ttctgtgatt ccttgaatct tgatatccat ctgtaaagca gtgataccat
    10921 cttttgtacc ggcaacttta aagtccatat cgccaagatg atcttccagc ccttggatat
    10981 cagttaggat cgtatagttt tcaccatcag aaacaagtcc catcgcaata ccggcgactg
    11041 gcgctttgat tggtacacct gcatccatca atgctaatgt gccagcacag atacttgctt
    11101 gggaagaaga accatttgat tccaatactt ctgctactaa acggatcgta taaggaaatt
    11161 cttcttctga agggattact tgtgctaacg cacgttcacc taatgcgcca tgtccgattt
    11221 cccgacgccc tggagagcct gcacgtcctg tagaaccaac agaaaattga gggaaattgt
    11281 agtggtggat gaatcgtttg ctgacttcca cccctaaacc atcaatgatt tggtgctctc
    11341 ctagaggtgc gagtgtacaa gctgataaag cttgtgtttg tcctcgtgtg aacaaacctg
    11401 atccatgtac acgtggcaat aagctgacct ctgatgacaa tggacggatt tcatccaatt
    11461 tacgcccgtc aggacggatc ttatcgatgg tgatcaattc acgtacgaca tctttttcaa
    11521 gatcttctgt gatttgttta acttctttca acaagtgatc ttcatcttca tgacctgcaa
    11581 atttctcagc ataagcttcg cggatatcga tcttcacctg ctcgatatta tcttcgcgag
    11641 caagtttttc ttcagtcatt actgctgctt tcattgtagc gtaagaagca tcaaagattt
    11701 cttttttcaa tacttcatca acttgcagta aagtcacttc cattttttct ttccctactg
    11761 cttgaacgat ttcttcttgg aaagcaacta actctttgat agcgtcaaaa ccaaacagca
    11821 atgcaccaag catgtcttct tctgaaactt ctttcgcgcc gctttcaacc atattgatgg
    11881 cttgtttcgt tccggcaaca gtcaattcga tatctgtttg ttctgcttgt tctactgttg
    11941 gattcaagac gtactcgcca tttacacgac caacttctac gcctgcgatt gggccgtcaa
    12001 atgggatatc tgagattgat agtgctaaag aagaacctag cattgctgcc atagctggcg
    12061 agcagtctgt ttctacactc atcacgatgt tcgttacttg gacttcatta cggaagcctt
    12121 ctgcaaacat cgggcgaatt ggacggtcga tcaaacgggc agtcaatgtt gcttccgtac
    12181 ttggacggcc ttctcgtttg atgaaacctc cagggatttt ccctgctgca tacatttttt
    12241 cttcataatt gatcgttaat gggaaaaagt ctgtatcttt tgcttcttta gaggccacag
    12301 ctgcacttaa gactactgta tctccataac ggactaaaac ggctccgttt gcttgttttg
    12361 ctagctgacc gacttcgatt tgtaagggac ggccgcccca tgtcgttttg aatacttgtt
    12421 tttctgacat aacttctcct tttccttctc gtgaaagcaa gacgctacta aacaaatgaa
    12481 aactgccgaa agccgtcttt tgagcagctt tcatttggtt agtaggagtc catctagctt
    12541 gatcacggaa ttttacttaa ttgatatacg agtaaaaaaa gtgagattca tgtgaatccc
    12601 actctttttg actaaaatta acgacgcaag cctaaacgtt tgatcaattc gcggtaacgt
    12661 tgaacgtcag ttttacgtaa gtaagctaac aagttacggc ggtgaccaac ttttttcatt
    12721 aggccgcggt atgaatgatg gtcttttttg tgcacgcgtg catgttcatt caagtggttg
    12781 atttcttcag ttaatacagc gatttgtact tctggagaac cagtatcccc ttcatggcga
    12841 gcgtattcgt tgatgatttc attcttgcgt tcttttgaaa ttgccatttc tttcacctct
    12901 ttcgattatt cccctgcgac tgagtaaaac gttggtgatt cgcttaacca agtggcaggt
    12961 catgtgccta gagcacacag ctatacttta cctgaaaccg ggttgaaaat caagtagtta
    13021 atcattaaga aaaggtagtt ttcaaacaat cttactacta cttttccatt tttgtggtaa
    13081 actataatgc gaaaatagac ttgaaacaca gtcttttaag aaaggggtac atattccatg
    13141 attacaatgg atgatatcat tcgtgaagga aatccgacct tacgcgaagt tgcaaaagaa
    13201 gtttctcttc cattaagcga agaagatatc tctttaggaa aagagatgct tgagttctta
    13261 aaaaacagcc aagatccaat caaagctgaa gaactacact tacgaggcgg tgttggctta
    13321 gctgctcctc aattagatat ttctaaaaga atcattgctg ttcacgtacc aagtcctgac
    13381 ccagaggcag atggaccttc tctaagtact gttatgtata atcctaaaat tttgagccat
    13441 tctgttcaag atgcttgttt aggggaaggt gaaggctgtt tatctgttga tcgagaagta
    13501 ccaggatatg ttgttcgcca tgccaaaatc actgtgtctt attatgatat gaatggtgaa
    13561 aaacacaaga ttcgtttgaa gaattatgaa tcgatcgtcg ttcaacatga aatcgaccat
    13621 atcaacggtg tgatgttcta tgaccacatc aacgatcaaa atccatttgc actaaaagaa
    13681 ggcgtattgg tgatcgagta gagaattggc aggttccttc gatactatgc tacaagaaag
    13741 ctttataaaa aaggggagtg ctcaaaagct gcacttccct tttttatgtt tctccactaa
    13801 tcgaaattta gatagacatc tagaaatcgc aattccaaaa aattctgtgt attggatacg
    13861 catcttttct tattttttct tatttcttcc atatgggtgt ttatctcaga gatgatagaa
    13921 tagtttctag gataaaaagt agcagctacg atgttatcct tcgtagagga ttcctgcttc
    13981 agctagtttt tgtttgatgt ttttcagcat atgcttttct gggtagcgga tcgtatgcag
    14041 atggatgccg cctgttaatt cggataacaa agtagcatta cttttttgat aacgtctcat
    14101 aaaggaatcg atatcttgtg gcgttttgat gtgcaaagtt cctgctaact caccgtaaag
    14161 gggatgttcc acaattacat caatgatttc accaccattt tcaacgatta gtctcagttc
    14221 ttcttctgtt tgatcttttc catgctggac agcgatttta ctgatgtgcc cagtattggt
    14281 ttcttctgtt tcgaggatat aacctcttgc tgttgccacg atacttttac ctgcagctcg
    14341 tagtaaagca atatcgccta caatgatctg acggctgact ccaaaagttt tcgcaaactt
    14401 gctagcactc actggttttt ctgtatttgc caaggtttgc atgatttgtt ttcgccgtgt
    14461 ctctccttcg atcacactta ttcctccttc attttgtttt ggtattcgcg cgagtgtttc
    14521 atcaaatcaa catacgtgtc tgttattgtt tccttatatc ccttattttt cagcagacta
    14581 acaactttat ccaatacttg ctgctctctt tttgcatcat aaactgcttg gttttcttct
    14641 tttttcactt gtcctatttt agttaccgta tccattcttt tttctaataa agcaaccaac
    14701 tctcgatcga tcatatcgat cttatgacgt aatgtttcaa gagactcttc tgttagttct
    14761 tcctctactg tcttgatgat acgagcagga tttcctgcaa tcactacatt gtcgggaaaa
    14821 gatttggtca caaccgaacc tgcgccgact actacgttgt cccctagagt gacacccggc
    14881 gtgacgataa ctcctccgcc taaccaaaca ttattaccga ttttaatggg ttttgcgtac
    14941 tccagaccgc tgttacgttt cactggatgt agtggatgcg tcgccgtgta aagctggaca
    15001 ttcggagcga acatgcagtt atcaccgatt tcaatcgtgc tgacatctaa aaatgtacaa
    15061 ttgaaatttg cataaaaatt ctccccaaca aatatattat atccgtaatc aaaattgata
    15121 actggctcca tatagatttt tttccctgta cggccaaaag tctctttcaa caattgggat
    15181 cgcagttccg ctgattctgc ttggttgatg atttggcttt gacttcttgc aaacatacgg
    15241 tcggcaacca gctcaggatc attggcaaaa taaagctcgc ctgcaatcat tttttctttt
    15301 tcggttttga attcagtcat aatctgctcc taattcttgt atttttttga aaacaaccgc
    15361 tccgtcttct tgaataaacg tataaggctc cgctaaataa acaggcgttt gattgattac
    15421 taagattttg gctgacggct ggcgaaactg taccagatca caaaacggat gaacttgaaa
    15481 actcgttcca acaatgacga tcagctctgc ttggctgacg gcataagccg cctgctccat
    15541 gactccatct tgaaaccctt caccatataa cacgatgtct gggcgtattt gtcctccgca
    15601 tgcttgatgt ttgtcgctgt gaagatattc tttccagtct accgtttgtt gacattttcg
    15661 acaatgacat tgatacagac tcccatgaaa attgacgagt tgcgggcttt tagcctttgc
    15721 gtgcaaaccg tcgatgtttt gggaaacaac ccaaacattt ttttctttcg ctagatgtgc
    15781 tatttccaaa tgaatgatat tgggttttgc ttcaggatga taaatctttt tgacaaattg
    15841 atagaattta tccggttcat tcaccattgc ttgatgactc agcaagtatt ccggctgctc
    15901 catcccgtgg tagattcctg acaaagatcg atagtctggc actccagaag gtgtggatac
    15961 ccctgcaccg gtcaaaaatg taatttgctg ataagctaat atctcttttg ctgcttcgtc
    16021 tactgttaat tgtttcatgt tttgtcacct cactttccat tatagcccat actcgaacaa
    16081 atttattgtt gaaaagtcag aaaaatggga ataaaaaagt gttatgggca tttgagcaat
    16141 ttatcacaat gttcactgac aaaagaatga aaacgtgtta caatactgct gtataaaagt
    16201 gaggtgaatc gatatgttac cattctttat ttattggggc gttattctgg cttgtatcgc
    16261 ctggttagcg ttaagcgttt atttctctgt tttttatctt gtacgcaaag aaaatggaaa
    16321 tctttgggct tttgctttat tcaacgtgat tgccgcaatt gttttagcca ttacattagc
    16381 cgtatatcga acatggggct ggggaatcac ccaatactca agtttgattt atctaatatt
    16441 agccatttat ggcgtcgttg ttcttttgca agcgatccta ggacgtgagc caaagaaagc
    16501 ggcagcttaa atgatcgaga tggaactgga cacaggaaat tttctgttct agttcttttt
    16561 ttattgtatt acatacattt ttgtgtataa ttatactatc gtttgcaatg gatagatgat
    16621 tcacattttg attgtttttt cggtaaaagt aaagatttct atatttatca gtattttttc
    16681 aaaaaccagc agttatagat tgacaaagcc tctcgagtat cgtaaacttt ttttgtataa
    16741 ataactgaat acgagatgcc tcaatcaatg aggtagaggt cgcgagaatc attaaccctg
    16801 tggagtgaag caaacatgga gaagcaggaa tagggaggat cgccgaaatg tgcacttttt
    16861 tgcttggagt gtatgttggg acgcaagtta agagcttgcg gactgtctta gcagtgatgc
    16921 taagttgcgc tatgttttgt gagaggttta tcaccttatg gaattttagc ggccttttta
    16981 tatctataaa tcggtcgctt tttatttttt cagataaagc tttaccgcat ttcatctaga
    17041 ccgctcgtgt atcatataat cagaaaggga gaaaaaaatg aaaaaaaata tggtttcagt
    17101 tgccgtacta tttgcttttg tcttctcact attcagtgtg ggatctctgg caaatgcaga
    17161 tgaaaaaaca ccgagcgatg agtttcgcgt aggtatggag gctgggtatg cacccttcaa
    17221 ctggtctcaa caaacagatg agaataatgc cttgccaatt caaggccaaa atagttacgc
    17281 cggcggttat gatgttcaaa tcgctaaaaa agtagcagat ggtttaggga aaaaattagt
    17341 catcgtccaa acgaaatggg atggtctggc tcctgccctt caatcaggca agatcgacgc
    17401 gatcatcgcc ggaatgagcc ctactgctga acgacgaaaa gaaattgatt ttaccgatcc
    17461 ttattacgaa tcacaattag tagtagtagt tcaaaaagat agcaaatatg caaaagcaaa
    17521 aagtttagca gatctttctg gagcaaagat cactgctcag ttaaatacat tccactatac
    17581 tgtgatcgat cagataccag atgtgcagaa acaacaagca atggataatt tctcagcaat
    17641 gagaacagct ttagcttctg gaatgatcga tggttatgtc agcgaacgtc cggaaggtgt
    17701 aacggcaact agtgtgaaca aagacttgga aatgcttgaa ttcagcggtg cagatggctt
    17761 ccagacaaac ccggaagatg ttcagatttc tgttggtatg cgtaaaggcg acccggatat
    17821 ccaaaaagta aaccagattc tttctggcat ctctaacgat gaacgaacca aaattatgga
    17881 ccaagcagtc aaagatcagc ctgctgcaac tgatagtgaa gaaaaacctg gcatcatttc
    17941 tgatttcact acgatttgga aacaatacgg caatatgttc ttacgaggtg ctggacttac
    18001 cttgttcatc gcattgatcg ggacagttgt cggtactaca cttggattac tgattggtgt
    18061 cttccgttcg gtacctgaat caggaaatgc actgactcgc ttcttccaaa aaatcggaaa
    18121 tgcacttctt tctatctatg tcgaagtatt ccgcggaaca ccgatgatgg tacaagcaat
    18181 ggtcatcttc tacggtctgg cactcgcttt tggcatttca ttgaaccgaa cgattgccgc
    18241 tttgtttatt gtctctctca atactggagc ttacatgaca gagatcgttc gtggggggat
    18301 ttttgcagta gatgaagggc agttcgaagc tgcgcaagcc attggtatga cccatggcca
    18361 aacgatgaga aaagttgtta ttccacaagt tttacggaat atcttgccag caacaggaaa
    18421 tgagtttgtt atcaatatca aagatacagc agttttaagt gtaatcggcg tagctgattt
    18481 gttcttccaa ggaaactctg cttccggtgc gaatttccaa ttcttccaaa catttacgat
    18541 cgtcgggatc atctacttag tcatgacctt tacgatcacc agaatcctgc gggcggtaga
    18601 aaagaaaatc gatggtcctt cagcttatac caagcttgaa gaaatcgaca attcaaattt
    18661 gcaggaataa ggaggaagaa tcatgaatga aaaaattatt gaaatcaaac atctaagaaa
    18721 aagcttcggt gaaaatgaag tattgaaaga tatcagcgta acagtcaata aaggtgaagt
    18781 tgtgacgatc atcggttctt ccggttctgg taaatctact ttcttgcgct gtatcaattt
    18841 actggaaaaa ccaacaggcg gcgagatcat ttataatgga gaaaatgtat tagctcctaa
    18901 atataattta cctaaatatc gaacgaatct aggtatggtt ttccaatcct tcaacctgtt
    18961 caacaatatg aatgtcttgg aaaactgtat gtctggtcaa acaactgtcc tgaaacgaga
    19021 taaagaaact gccaaaaaag tagcaatcga aaatctggaa aaagttggta tggatcgtta
    19081 tatcgaagcc aaaccagcac aattatctgg tggacagaaa cagcgtgtcg caattgctcg
    19141 ggcattatca atgaatccag atgtattgct attcgacgaa cctacttcgg cgttagaccc
    19201 tgaaatggtt ggcgaagtgc ttaatacgat caaagactta gcacatactg gcttgacaat
    19261 gcttatcgtg acgcatgaga tggaatttgc aaaagatgta tctgaccgtg tcatttttat
    19321 ggataaaggt gtgattgcag aagaaggtac accagaagat atctttgtcc atccgaaaga
    19381 agaacgtaca aaagaattcc tttcacgtat tttaaatgct taggatcaat agctgattgg
    19441 gacaaagagc tgcggtgttt accgcagttc ttttttatga attactcata aaaggtaaat
    19501 ttctgaaaa
//
LOCUS       DANKKG010000044        19443 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000044 DANKKG010000000
VERSION     DANKKG010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19443
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.044"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            98..742
                     /locus_tag="PW004_001312"
     CDS             98..742
                     /locus_tag="PW004_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDL2726295.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(798..1301)
                     /locus_tag="PW004_001313"
     CDS             complement(798..1301)
                     /locus_tag="PW004_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HDL2726296.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(1314..1360)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1898..2863
                     /locus_tag="PW004_001314"
     CDS             1898..2863
                     /locus_tag="PW004_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HDL2726297.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(2962..4134)
                     /locus_tag="PW004_001315"
     CDS             complement(2962..4134)
                     /locus_tag="PW004_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2726298.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(4121..5266)
                     /locus_tag="PW004_001316"
     CDS             complement(4121..5266)
                     /locus_tag="PW004_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2726299.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(5280..6212)
                     /locus_tag="PW004_001317"
     CDS             complement(5280..6212)
                     /locus_tag="PW004_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2726300.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            6329..7408
                     /locus_tag="PW004_001318"
     CDS             6329..7408
                     /locus_tag="PW004_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDL2726301.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            7405..8023
                     /locus_tag="PW004_001319"
                     /pseudo
     CDS             7405..8023
                     /locus_tag="PW004_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(8133..8360)
                     /locus_tag="PW004_001320"
     CDS             complement(8133..8360)
                     /locus_tag="PW004_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL2726302.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(8463..11051)
                     /locus_tag="PW004_001321"
     CDS             complement(8463..11051)
                     /locus_tag="PW004_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HDL2726303.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(11071..11730)
                     /locus_tag="PW004_001322"
     CDS             complement(11071..11730)
                     /locus_tag="PW004_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDL2726304.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(11779..12282)
                     /gene="trmL"
                     /locus_tag="PW004_001323"
     CDS             complement(11779..12282)
                     /gene="trmL"
                     /locus_tag="PW004_001323"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HDL2726305.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(12503..13369)
                     /locus_tag="PW004_001324"
     CDS             complement(12503..13369)
                     /locus_tag="PW004_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDL2726306.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            13478..14119
                     /locus_tag="PW004_001325"
     CDS             13478..14119
                     /locus_tag="PW004_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HDL2726307.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(14181..15542)
                     /gene="mgtE"
                     /locus_tag="PW004_001326"
     CDS             complement(14181..15542)
                     /gene="mgtE"
                     /locus_tag="PW004_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HDL2726308.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(15560..16459)
                     /locus_tag="PW004_001327"
     CDS             complement(15560..16459)
                     /locus_tag="PW004_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDL2726309.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(16461..17258)
                     /locus_tag="PW004_001328"
     CDS             complement(16461..17258)
                     /locus_tag="PW004_001328"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HDL2726310.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(17236..17940)
                     /locus_tag="PW004_001329"
     CDS             complement(17236..17940)
                     /locus_tag="PW004_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HDL2726311.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            18071..18646
                     /locus_tag="PW004_001330"
     CDS             18071..18646
                     /locus_tag="PW004_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HDL2726312.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            18764..19441
                     /locus_tag="PW004_001331"
     CDS             18764..19441
                     /locus_tag="PW004_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HDL2726313.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         5840 a         4118 c         3462 g         6023 t
ORIGIN      
        1 ttgtcaattt gtgaaataaa ctatctattg ttttggataa aattatgata tgattaattt
       61 gaaattaagc taaaatgaga caattggagg tatagcgatg ctattaaaaa cacttgtata
      121 taagaaaaaa gatctgacaa ccgttacaga gactagtaca ttagaagaag cattaaaaat
      181 tttagaaact tccggatacc gttgtgtgcc gatcttagat tcttcaggta atattttccg
      241 tggaaacatt tacaaaatgc atatctatcg tcataaagca aatggcggtg atatgacgct
      301 tcctgtcact catcttttga aaaacgctac aaagttcatt tacctggata cttctttctt
      361 caaagtattc tttacgatca aagagcttcc ttatattgct gtccttgaca gtgaaaatca
      421 tttttatggt attttgacac atagtacgtt gcttaatatg ctctctcaat cttggagcgt
      481 agaacaagga agctatgtac tgacaattgc ttccacagga aagcaaggag atttggctgc
      541 tattgcaaaa atcatttcaa aacacagcag cattgccagc tgtattactc ttgatatcga
      601 aaaagacgaa tacattcgac gtacattgat cacgctaccg gctaacactg aagcgaaagt
      661 atgtgaaacg atcgtcaacc acttagagaa aaagaattat aaagtggtag aaattgaaaa
      721 tttgcaaaac caagaagcat aatcaaaaag cgaaaaaaga aaacagctaa tctgctttct
      781 tttttcgctt tttataatta gacacgcttt tcaaaatcaa gcgcgcgatc taaataatac
      841 atcaatggtg ctgaaacagc catgacgata aactcactta atgcaagcgt gccataggtt
      901 gtccaaaatg cttggccacc tgttggaact aacatataag caatcagtcc catgctgatg
      961 gtaaagaaga tcgtgttcaa tactaaacgc atctttacat ttttcacttt gttttgcaat
     1021 aaagctgtca ggcctaaaga aagcaatgtt tggccgccac caaaaaccac atccatgata
     1081 ccaaaaccaa agaaggcatt atagacgatg accccgccta aaactcccca aagcaattta
     1141 cggttaaaga caactaaatg attcaaactt tctgaaatac gaaactggat cggccctgag
     1201 gatacaggcg caactaaaat agttaacgct agataaagag ccatgatgat gccatttaca
     1261 acgattacgg ataaccgttg tctgcttttc gttttttgca ttttctctcc tcttttctcc
     1321 tagttttttt aacgcgggat ggttgatgaa ccgcggttat gtgaaacaaa caataattat
     1381 aacagacttt tcatttattt caatccgtct tatctgcttt taaaaaagca aaagcttaaa
     1441 caatgtaaac gtttgctaaa aatcgtggga tatggtataa agtaagtgta aggggtcata
     1501 agaattccca gtaaagcaga cgaagtagga tctgcaagaa cagaattgtt gtgaaactta
     1561 aataaataaa aaaggatgtg ggaatttgag caacctctta ctagagaacg gtctagcaag
     1621 cagctgaaga tctaagtgga catgccgaac cttggaactt ctccttacgt aagtaatgga
     1681 tcatgcgctt gctaggcttt tttgtgtttt tcggtcttcg gacctttttc attttcctag
     1741 acttctgcta taataaccaa gaattaattt tgtaacagat ttttttgaaa aaacaagttt
     1801 ctttcattca aaaaatagaa aaagcttgct aattattggt taaccagcaa acaggagtat
     1861 taaaatatct tgttttattg aatagcagga ggaaatcatg aaattccatt atcatatatt
     1921 gatcaatcca tcggcaggca gcggaaatgg ctataaagta gcagaaagaa ttttaccggt
     1981 attaaaaaat aagcatattg actatacact tcattatagt gaatacaaag gacacgatgc
     2041 tgagattgct gaaactttgg caaaagaaac attgattcct tggatagaag aagaacagaa
     2101 aacaatcgat actttccccc ttttgatcat cgtaggaggt gacggcacct tacatcaggt
     2161 acttgatact ttctatcaga tggaagtaga attcccagtt gcttatattc cagcaggttc
     2221 tggaaatgat tttgctcgag gggcaggcct tccaaaaaat cccaaaaaag gacttcagct
     2281 gattttagca gctcaatcac cagaaaaagt ccacataatg gcttatgaag aaaaaataag
     2341 tgaaaaaaaa ggaatagctg ttaataattt cggtatagga ttagatgcag ctatcgtcca
     2401 tgcgacaaat cattcttcca caaaaaagcg attgaacaaa tacaacttag gatcattttc
     2461 gtatttattc tctattctcc gtgctttatt cactcaaaaa ggatttccaa ttctggtaga
     2521 tgcaggagga aaacaattaa gtttttcaaa cgcattttta tgtacagcaa cgaagcatcc
     2581 tttttttggc ggaggaatcg cgattgttcc aactgctgat gcgtctaaac cagtcattga
     2641 ttttgtcgtg gtcgaacgaa tcaatttctt taaaatcttg tggctcattc ttttacttat
     2701 acagaaaaaa caattgaaat cgaaatattt ccatcactac acaacaaaca gattacgtat
     2761 cgtttcaaca acaccgcaac atggtcaaga agatggagaa gaaatggaaa aacgaccttt
     2821 tgatatcaca ataaccacaa ctaaacagtt gttttggtgt taataaaaga ggttttgtca
     2881 gaagctacat ctggaacacc gtttattcgg aaaaaaggac tgtgacacga cttttgtcac
     2941 agtccttttt ttactatgca gttaattctt tgcccaattg ggttgccatc gatagtttct
     3001 ttctttaccc aaaataaatt ggatcattga aaacaccgca gcaaaagcaa ctaagacaca
     3061 accattgaac caaaaagatt ctgcaaatgt ttgattgtaa ctgagtgttt cactgatcaa
     3121 gttattattt tgaacaaacc aatatgttgg tgtgaatgcc gctattttgc taaccatttc
     3181 tggcaagtat tctgctggta caaacacccc accgataaag caactgcctg tgataaaaat
     3241 atctttgatc ccaccgctgg tttctgagtt ttttaccaat gaagtgatac aagccgaaaa
     3301 gctaatgatc ggcaataaaa acaagaaagt atttaaaata tgataccata ccgttatatc
     3361 ccacgaggtg tggatcacta acagaaccat tagaagtaag aagccactga acaacagcag
     3421 cgagtaacta atcaaactaa aggttacttt gcgattcaag tttcgtttgg taatgggcgc
     3481 actctggttt cgtttactga tctctttgcg attaaacgct agattgatga cgctgaaacc
     3541 gctaaaaatg gatagaaaca acccataaga caagaaatta aaggttgtgc ccgtcaacag
     3601 ctttttcatc cgctgactat atcccgcact tagctcaact tctccagtaa ccgagagtgc
     3661 catttgtgtc tgttccatag ctgcatcgat tgttttatct tcttcttgaa acagaatcag
     3721 tgtttgcaaa aaggtgttca cttgctgtgt aacaactgtt ttagaaaagg catctgggcg
     3781 actctgtatc ttgatcgtcg ccatctttcc tacttcgact tgttcggtaa agtctgcagg
     3841 cagatgtaag ataatgttta cacgattaaa ataaagagca tcatcgattt ctttttgtga
     3901 ggttccatcc aatgtaacga ctgtttcctt gtcattcaaa tatttacgaa ggccatcgac
     3961 tacaggcgac tcttcttcac taatcaaggc gattttcgca tcttgcagag aggtattttc
     4021 aacatcaata ttttgcgcaa aaaagaatgt ggcaacaaca gtgataatta cacctatcag
     4081 taaattcgat ttatttgctt taagcaatgt ccaaatcgtt ttataaagcg tcataacgca
     4141 cccctctttc catccaagca tttcctaacg caaaaaggaa aataaatccg atcaaccata
     4201 gaagattggt ataaaaacta gccaaatcag tgtagaaata aagtttatac aaactttcgc
     4261 tgatcaagtt gacgagattt aattgaccaa caatcggaat atgaagatca atccaatact
     4321 tcacttgttc cgaccccatc ataccagcaa aaaagctcat cgcaatcgtc actgaaatca
     4381 agatgctatc cttttgcacc agattcattt ttggtatcaa attaccaacc aatgtaccta
     4441 acaaaatcgc acacaacgac cccagagcgg taacaatcat caaccattgc caacgttcac
     4501 caaagtctac ttgatagata aaacgaaaga cagctaacat aatataaatc tctgtcaaaa
     4561 caatcgtaaa gcttgctgcc aaattagcga ttgaaactaa cgattgcttt ctaggaatca
     4621 aggacaagcg aataccattt gacgattgat ttgcttgctg atcctgtaca ttgcggaccc
     4681 cccacatcgt cccgtagaga atcgccattc caatcagagt gaagaaataa aaacttttga
     4741 aactcatatt acgacctgca tgaacttctt gaaaagatac ttcttgttca aaaacagtca
     4801 tgatgggcgc gagatcaacg ttctccactt ctgccacttt actcgaaatt ataccaatac
     4861 gctgtaaaaa ttgatccatg acgtttttca tcaacgtctg actcattcct gcttgaccca
     4921 catggagcga aacagatgaa ccatcaatga gataaaaacc agccaaatca tctgcattta
     4981 gcttttcatt cgcttctact tccgatagtt cctgtacttc aaataatggt ttatcattga
     5041 cttcgacttg ttctaagact gtggtaaaat tttccgaaag cgctgtttga tccgctttga
     5101 ttacaattcc agtattaatc gtttcaagat tgccaccttg atcaatctcg ccaaaggccg
     5161 ccataaacat caatcctaaa agaattggaa aggccaatgt ccaaaacaac atggatttat
     5221 ttcgcagcag tatttttgtg cgatatttat aaaaatgcca aaacattatt tcccctcctc
     5281 tagtctctca aatccttgcc agtaatctct aaaaagacat cattcaagga cgcttctttg
     5341 gttgctattt ttccgtaact gatcttttct tcttgtaaga ttgtaagaat cggcaacaat
     5401 actgtattta gttcagtagt tgttacttgg agctgacctt ttttataagc gattccatca
     5461 gaagctattg ttcgctgaat cgctgacaac acattttctg ggagtaatgg gatgtcgatc
     5521 gtcaaacgat ccgccgtccg taccatatct tttagagatt ctttggttcc tttagcaatc
     5581 acctgtcctt gatccatgat cacgatctgg tcgcaaagaa tttctacttc ttccatgtaa
     5641 tgtgttgtat agacaatggt tgctccttgt cgattcagct ccttgatact ttctaaaatt
     5701 ttattccgac tttgggggtc tacagctact gtcggctcat ctaaaaaaat taaccgcggc
     5761 ttgtgggcaa tcccacatgc gatgttcaac cgacgtttca agccaccact aagttctcct
     5821 ggacgaaacg aaacatattt ttctaatccc actaattgaa tcacctcttc aatcaaggct
     5881 ttccttttct ctttttcgga aacatataaa ccgcaaaaat aatcgatatt ttcttgcaca
     5941 cttagttcgt caaagaccgc aacgtcttga ggaacgaccc caatcattgc cttgacagca
     6001 taattttcag gagtcattgt tgtcccgaac aatttaatgg ttcctttgtc ataatttaac
     6061 aaagaaagca tacagttgat ggcagtcgat ttcccactgc cgtttggacc taacagacct
     6121 aagacttctc cttcttgtac ttgcagatca aaatgattca atgctacaaa atctccatag
     6181 cgtttgacta atttatcaat ttcgataatc atcatgaaaa actccttcca aatttcttgt
     6241 tgatctgact ataataaagt gcaagaaaag aaactagtga cgattggcta aaccacacat
     6301 gacatttgtc acaatgaagg agtctgctat gcataaatct cggctactca acgaaactag
     6361 ctttctactg gttatctcat tgcttttgct ggtaagtcaa ggattcgttg ctatgcaagt
     6421 tatctattta ttgatcgccg ttttattcac tggtgtcagt tatttactgc ctacaaagcc
     6481 aaattggatt ttccaagtta ttctatttct tttatgtgcc tttttacctg atcttgtcct
     6541 gtttctgcca gcgacgatgc gagtgatcgc ccctgaagaa agccaccttg attacttgtt
     6601 tttgttaggt tttttgttga tttcgacggt tggtaatcat ccccccatca aggtcgcctt
     6661 tgtcgtaatt ttgctgtttt tttcctttta cttacgaaaa aaagatcaac gaacgaccga
     6721 tcttgagaaa aagtacattg tctccaaaga tactcattgg gaaaaacaac aagagttatc
     6781 ggaacaaaat aaagcattgg ccctgtcaca agaaacacaa ttagccttgc aaattgtgga
     6841 agaacggaac cgaattgccc gagacatcca tgacaatgtc ggtcatctgc tgtcaagtgc
     6901 gattttacaa attggtgcgc ttgaaatgat caatcaagaa ccaaaactaa aagaaccatt
     6961 agaaaattta agtgccaccg ttcacactgg gatggatcgg attcgtcaaa gtgtccatga
     7021 cctccatgag acatcgatct cttttcaaca ctccttagag tttctgatcg ctgattttcc
     7081 ttcttctgtt acgattgaag gaaacttgtt tgaaggattg aatgaaacgc agcaaaactg
     7141 cttcttaatg gtcatcaaag aagccttaac caacagtatc aaacatagtc atgccaaaac
     7201 cgttgtttta cattttcgaa cgttgccggc cttttatcgc ttgcaaatca cgaatgatgg
     7261 caccagccca caaaaaaatt ctgctcccgg tattggtttg aaaacgatgc gtcaacgtgt
     7321 tcaagaacaa aagggacaag tccatatttt ccaagaaaac cagcaatttc agttaacgat
     7381 cattttacca agggagggac accaatgata acagtagtca ttattgatga tgatccattt
     7441 gtgacgaaat cgttacaaac cattttagaa agcacgaaag agatccgtgt gttaggcatt
     7501 ggtcattgtg ccaaggatgc gcgaaaccca tcgtcccgat gttttgctga cggacatccg
     7561 catgcctaaa caaacgggca tcgatgctgc caaagaaatt ttggctaaat ttcccaaagc
     7621 catccttttg cttttgacga cattcaaaga tgaagaatac atccgcgaag cattttccat
     7681 cggtgtcaaa gggtatttaa tcaaacagaa cttgcaagcg attattcctt ctgtcaaatc
     7741 cgcttataat ggacaggccg tatttggtaa tgaaatcatc gagactttca ctcaaatgat
     7801 gaagaataaa ccgacgattg atagtcatgc ttatgcttct tttagtgaac gtgagcttgc
     7861 aatcatcaaa gaagtagctg ccgggaaaaa caataaagaa attgcagatg ccttgtactt
     7921 gagtgacggc actgttcgca attacatcag ccaattacta gaaaagctag atttacggga
     7981 tcgcacgcaa ttggctatct atttttatca gcatttgcaa taagtcttat ctaacacaaa
     8041 aaaacctgaa tgatgctcct tcgcatcatt caggtttttt tcacatcttc tattcaattg
     8101 tcccgcaaac catttcctca cgattgaaag aatcaaggat tcgcagggct gccaggattc
     8161 gttggattgt tcggcgtgtt aggcggaaaa ataggcgcct tgtctaacgt tttttctagt
     8221 tttgattctt cacgatcgtc tcgttctaca tcaggacttt cattcaattt gaattctttt
     8281 tccatctcat ctaacttttt aaagtcattg ccagattctt ttgctaattt cgctaattta
     8341 tcttcggttt ctttagccat aaagtaacgc cccccttttg atttcagctt agcatatcac
     8401 actgaaaaaa caaaagaaaa cgctaaccaa cgatcatttt tagctatttc tcatctttaa
     8461 aatcaagcat tttttgccgg tataaaatcg tagggagaac tgttttccat accaatcatc
     8521 ggatctacta actggttatc gatcacttct gctgtaagaa cgaaatcttt agaaacagca
     8581 ggttctacct cttctttctt ttcttctcca acaaatgaag aaaacagatc tggttccact
     8641 gtttccatta cttggttttt cgtttcattc gtcccactgt catcttctac aggtagcttt
     8701 tcttttacaa cccgtggctc ttttgtatag ttcccatctt caaatggatc atttccgacc
     8761 aaagaatctt cataagctgc tagtttggtc cggagagtga tattcatttt atcattatcc
     8821 atgatccttg atttcaacgt agtcatccgg cttgctgatt cattcgctac acaagtgaca
     8881 taggcttgct cttcccctaa caaaatcaac agttccttac tgcgagtcac tgccgtgtag
     8941 agcaaattac gctgaagcat ccgctgatat tgatggacca tgggcaaaat aaccatcttg
     9001 aattcactgc cttgtgattt atggattgag caacaataag aaagagtgat tttattccat
     9061 tcgtttcttt tataagtgac ctcatttgca tcaaactgga taactaattc atccacttta
     9121 tcttcagaat cttttgccaa gatgataccc gtgatttctc ccatatctcc attgaataca
     9181 ttcaattcgg gagtattgac taattggagg actttgtctc ctatccgata aacggtgtcg
     9241 ttccaatgta cttccttttt tccaggtttc cctggattga atatctcctg catcatttta
     9301 tttaacgcat cgatccctgc aggtccccga tacattggtg ctaatacttg gatatcttgt
     9361 gcacggaatc ccttattttt cgctttttcc acaattttag agatcaaggg ttcaatttga
     9421 taagcatcac ttcggaaata ggaacgatct ttttgattct ttgtgaaatc ttttggcaga
     9481 cgtccttctt tgatctcatg ggcaagagga ataatactag aaccatcacc ttgccgataa
     9541 atctgattga gttccatttt gggaatttgt tcgatttcca aaagatcatg gagtacttga
     9601 ccaggaccga cagagggaag ctgatctttg tctcctacta agataacttg catattcgtc
     9661 ggaatcgctt tgaataatgt gttagccagc catgtatcga ccatagacat ctcatcaaca
     9721 atcaaaagtc cgccttctaa ttctttcgta ctgactgaag gtgttttttc ccgtccattc
     9781 agccccagaa gacgatggat cgtactggca ggcagacctg tcgtttcgtt cattcgttta
     9841 gctgctcggc cagttggtgc tgcaagtaga ataggaaaaa tttcttgggt atattttgaa
     9901 ggatccaatt ccagtccgtt caactctgca aaaagattga cgattccgtt gatcacagta
     9961 gttttccccg tacctggtcc tcctgttaaa ataaatagag gggaacggat tgcttcagcg
    10021 atagcttgtt cttgcgaagg tccatactgt atgccgaata ttttttcgat acgccgaata
    10081 ttcttctgca cttctttttc aggataatgg atttcttttt tacgactgag aaggcgctgg
    10141 atcgaattac cgatccccca ttcagaaaag tacaaactgt tttcgtaaag attagtctct
    10201 tcttgttgta tctttccttc ttcaacaagt tggataatta cagtagcaac ctgatcaggc
    10261 tggatttcaa ttgggcgact tgtttctaaa atacggatcg tttgctgaag caatgtctcc
    10321 gccggtacgt atgtatctcc tgtttgaact gcatgctgaa aaatctgatg caagatagct
    10381 gctcgaattc ttctcggtga atctgctgca ataccgattt gttcggctag attatctgca
    10441 cgtttgaacc cgattccttc tatgtcttcg actagctggt acggattttc ttcgatgata
    10501 tccaaagact cgttcttata ggcctggtag atactgaaag ctaactgact accgaacccg
    10561 taacgattca gtcccatgat cacttgatcc atcccgtggt ttagccgaat cgtctcagca
    10621 agcatttctc tttttggctt cgtcaaaccg gagatttgtt ttaataccga aggatcagca
    10681 atgatttttt cgatagcatc ttctcccagc aattctacaa ttttttctgc tgttttcttc
    10741 ccgattcctg ggaatttttc gctggaaaga tacgctacga tcccatttgc agttgttggc
    10801 ttttcttgct catatctttc gagtttaagc tgccttccgt atttaggatg atccactaat
    10861 tctccataaa aacgataagt ctcattttct tgaatatcgc cgaaacttcc ggtgactaca
    10921 atctcttttt cacggtaatc agctgtggtt tcactgatgt gtaccaaaag cactttgtaa
    10981 aaattactag gattttgaaa aaaaatcgcg gcaactgtac caacgaaata aggcatttct
    11041 tctggctgca tcggaagccg cctccttctt ctatagtaaa ggttctggtg ctgtttcagt
    11101 tcctaaaaaa tctgtttggt tccatacttt cagttcatat ggcaataatc tgcaaggctc
    11161 ttcagtctct aaaatcgtca aactactatt gaacaatcca cccatttcac gcaactcact
    11221 tagctctttt cctgtcatcc attgaatggc tgcagttaaa gaagctccat gtcctacgaa
    11281 taaaagtggg ccatctttat gtcgggcaac cgcatctgct acaacagttt cgatacgtgt
    11341 gattgccgat tcaatcgttt ctccgtgaaa aggagaagga tcatacagat ctaaatgatt
    11401 acgcaaattc ggcagattat ccggatattt ctggtacatt tcatcgatcg actgaccttc
    11461 taaagtaccc agccctagtt ctcttagctt atctgtataa aaaatctcta ccggacgagc
    11521 aagctctttg ttgatacctt ctgctgtatc acgtgctcgc tttgctgtac tggaataaat
    11581 tgctttaaaa ggaacatatt ttaccttttt cccaaatgct ttgatttctt cgtaactttg
    11641 tggcaaaagc ggagaatcgc cattgctccc ttgaaaacgt ctttcctgat tccattgtgt
    11701 cttcccatgt cgggtaaaat atagttccat acgcagcaaa ctcctgtctt tcttttattt
    11761 tactctataa taaactgtct aatccagctt gtcattttca tagtgatgcg tcaattctaa
    11821 attagggaat ccttgctggc gaagtgcttc atacactatc attgcagctg tattcgataa
    11881 attcaatgag cggacatgct catcattcat agggatgcgt aaacactttt cttcgttttc
    11941 acgcataaat gtttctggca aaccggttgt ttcttttcca aataaaaaga agtgctcttc
    12001 tccatcagca aaattttcgt cactatatac tttatgggca aattttgaga tcaaatgtag
    12061 tttacgatta cctaaaaatg ttaaaaaagc ttctaaatta ctgtggtaag taatatttac
    12121 atcattccaa taatccaacc ctgcccgttt tagatgtttg tcatcagttg aaaaacctag
    12181 tggctcgatt aaatgaagcg gagtattcgt ggctgcacac gtacgggcaa tatttccagt
    12241 gtttgcaggg attaatggtt caaataaaac gatatgattc atcaggatca ttcctctcat
    12301 atttatgttc gtttctattt tctgacaatt tgatttttgc ataaaaaaaa acgtatcaac
    12361 tcggtgtcag tcactgcgag tcgttacgtt agtgaagctc ttctcactaa cttttatcag
    12421 aacaggaacc tgataaaaac caatgaaaaa acaacttgtt gctaatataa caccctcaac
    12481 ttttttttgc aacctctact cattaaaaac tgctagaaaa acgttttctc ttagttcctt
    12541 ttaataattc tatgtcaatt gtaatcttct tcaaaggatt cttatataag tctgctttga
    12601 ttcgctcttc tacctgccat tctaaaggtg acatggccaa aagatctttt tgattttcct
    12661 ctggtatctc aaattcatag gttacacgct gacgttcagt taatttcatt gcttcagcaa
    12721 acttggctac tacacgttca ttggaataac tctgtttttc aggttgattc ggataaaaag
    12781 cctgacgtag ttcctttaaa taattttctt gtggcaccac cttcaacact tctccatctt
    12841 ctgagagtac acgttggaat tcttggtaat gagaaggcga aaagatattt aaaaccgcgt
    12901 tgaatgtttg atcagcaaac ggcaaattgg tcaaatcagc tacacaccaa aatgcttcgg
    12961 cagattgttc tgtagctgcg tagactcctt cttttgaaat atcaaatcca acagcatttt
    13021 gaatcttttt caattttgaa agttcattca agaagctacc ttctccgcag ccaacatcta
    13081 atagcgagac ttcttcaggt aaatgctgcg caatagtctc aataacgggc tgatacatgc
    13141 cagattggat cattctacgc cttggaacga acattccttg atcgtattct gttttaaccg
    13201 catgggataa aaaataaagt gttccttttt tggaaaggtc aaaacggtga ttatttttgc
    13261 atatcaagcc attatcagac ttttttactt cttcttggca aaccggacag cggaacagct
    13321 gatgattttc tgccaaaaac tggcgtcctc ggtcaatttt ttttaacata gttccccctc
    13381 atttctgttt tatcttaccg ttagatgctc actttggcaa ctgctaaaaa tatgatacaa
    13441 tgcctagaag aaagtgaaag ataaaggaga agattagatg atcaaagaat tttgcgctga
    13501 aaactacaca tctattcctc tagcaattgc tagaggagcc gatcggatcg aattgtgtga
    13561 caacttagcg gtcggtggca caaccccgag tactggtgtg atcgaagaaa cgttaagtta
    13621 tgctggggaa aaagatatcc ccgtaatgac gattattcgt cctagagggg gcgacttcgt
    13681 ctataacgat atcgaattga aaatcatgca tactgatttg attgaagcaa agaaattagg
    13741 gacggatggc gtagtatttg gttgtcttac tccttcaggc tggctagacg aagaggcctt
    13801 agaactattg atcgaaacag cagaagggtt gcagatcacc ttccatatgg cttttgacag
    13861 tattcctgaa gatcggcaat tcgaagcaat cgattggctg gtagaacatg gtgttgaccg
    13921 tatcttgact catggcggtc ctgctgacaa aactattgta gaaaatttcc cacggcttaa
    13981 agaattgatt gcctatacag atggccgctt aatcattttg cctggtggtg gtgtcactgc
    14041 agagaatgca gcacatgtag ctaaagaatt gaacgtaagc gaagtacacg gaaccaaaat
    14101 cgtggatctt cagttataat cttataaaca aaaaaacatt tgcaacaggc ataatgctta
    14161 ttgcaaatgt ttttgttttt ttacataaaa tagcccataa ataaagaagc aatattgaaa
    14221 taaatcagta cactcgtcaa atcgctcaaa gtcgtgataa agggaccgct tgctactgct
    14281 ggatcaaatc ctagacgatc cattagcatc gggattaaac tacctgcaag attcgccact
    14341 gtgattgcaa atagcatagc catccctatg acaaacccca agataaaatt atgtttccaa
    14401 aaacctacga ttagaaaaat cgtgatcccc gtaacagctc ctgtgacaac gccggtcaag
    14461 acttcagcca aaacaagtcg aacaaatcca tgatctttct catcagaaat cgcaagtcga
    14521 cgaacagcca cagctagcga ctgggttcca gcattaccgg cagttcctgt aatcagcgaa
    14581 atgaagactg ctaaaatact cgcttcactt accaagtctt catagtgact gattaaagaa
    14641 gcggtcgaca tacctaaaaa caacagagtg atcagccaag gcaaacgtcg agacgctgcc
    14701 tttactggat tttcattaat ttcttccaca ttgaccccgg caagtcctga ataatcgcta
    14761 gcagcttcat catcaataac gtcgatgata tcatcgactg tgacgattcc taacaaatga
    14821 tcatcataat cggtaacagg aagagctaag aagtcataat ctctaaatgt ttgtgccaca
    14881 tcttcttggt cgtcgcctac atgaactgaa agcactcgtt cgctcatcag atcagaaatc
    14941 attgcgtcgt catcattcac gattaggtcg cgcagagaaa taacccctac tagctgacct
    15001 tcttgattaa cgacatagat gtaatagatt gtttcagcta catctgcttc gttcttcagc
    15061 acgtacatcg cagagcgaac agtttgattg gctacgatcg aaacaaattc agttgtcatg
    15121 atcgcgccag ccgtttcatc ctcgtaatgg agaagttcct tgatttcact ggcatcgtct
    15181 gccgacatca aacttaaata tttagcaatt tgctttttat ccagcgtatt aagcaagtca
    15241 acagcattat ctgtatacat ctcagaaagc atatcagctg catagctagg acgcatttca
    15301 tctaggtatt ctttcatatg ttcgttgtct tcttcaatca catcgaacat atccgctaat
    15361 tctttaggcg acagatagga atagatcagc tgcctgttct cttctgtaag agattggtag
    15421 aattgcccct gctcatagat atgcatctct aaaaaactgt ttctgaatgc ctgaatatta
    15481 ttttccctta aatcttgagt caactgggca aatctttcct ctaattcttg attttcatcc
    15541 aaaccaatct cacctcgcct tattcttcaa accgtctcac aactgcctcc atgtctgcgg
    15601 ctaaaggctg cttgaactcc agcaactctt ctgtaaatgg atggtaaaaa cgcagatcat
    15661 aacaatgaag ggcttgtctt tttattccca gatccattcg cccgccatac atatcatcac
    15721 cgagcagggc acagccaatt gcggaaaaat gaactcttat ttgatgtgtc cttcctgtat
    15781 gcagctggat atcaactaac gcgatttctg ttagtcgctt ctctatccag tactcagtct
    15841 gtgccgaacg tccttcatca gagaccattc gcttcaatag cgaagaataa tctcgggcga
    15901 tcggcaaatc gatatgtcca tgtgccgata aatctgaaag ccttccactg actaaggcct
    15961 gatatttttt cacaaacttc ttttcacgaa gttgcatatc taattttgcg tgcgcaaatc
    16021 catgctttgc aaaaagcatc aagccagatg tatcccgatc tagtcgcgtc accacatgga
    16081 ttacttggtt ttcgtaacct tgttttttat aataagcctt tacacgatta gccattgtcc
    16141 catttggatg gtattgggca ggaatagacg aaacgcctgc tggtttattt actaccagaa
    16201 cgtgttcatc ttcatacacg atatcgatcg gtgtttcatc caaaagtacg gtttcatgtt
    16261 ctccttcagc aggaatcaca atggttacct tatcatcctt agctagagaa aacaagacat
    16321 tttctacttg atcattaact ttgatttgac cgccttgaaa ttttattttc gctaacagac
    16381 cctttgagat ccctttttct tttaaaaaat attttacttg ctgagaatga tctttctcat
    16441 aatcccatgt aaactgcata tcaatcctca ctaataaagg catctttcac acgatgccaa
    16501 aaatgcatat gtcgataaga agcaaaatgg atccgttctt ctgctattcg ataataaatt
    16561 gagcggatat ttgcttttgc gacattcaat tgatcaaccg ttacaagata atcttctgta
    16621 ttttccaatt ttatttctag ccattcatca tgggcaatca cgatcggaga acccaaagta
    16681 cgaaacactc gattattcag tgaagcaatc tctgccaact gaaaggcatt gatactagga
    16741 tggatcacag caccgccgac acttttatta tatgctgtcg aaccagtagg cgtagaaatc
    16801 gtcaatccat ctcctctaaa actttcaaac agctcgtttt tgatgtatac atcggctacc
    16861 atcgtacgat tggcccgttt aatcgttgat tcattcagtg ctaaaaaatg ctggtccggt
    16921 gtttcatcca aataactgat ccgaacatcc agcaagggat aactaacact ttgttcccgg
    16981 ttcgttcgta agctgtctac tagttcttcc agctcgtaat cccgccaatc cgtgtaaaac
    17041 cctaaatgac ctgtatgcac gcccaagaaa cgcacatcat tcagacaatg actgtagtga
    17101 tggaacgccg acaataatgt gccgtctccg ccgactgaga tgaccaattc tggctcatga
    17161 tcatctcgtt gaatccctgc ttgttctaaa agtgcgttca aacgttctgt cacttctata
    17221 gtctttgctt ctttattatg aacgatcgct actttcatcc cctgcctcct ctctttcgct
    17281 ttctttcttc aaaagcgttc gatagtagac atcatctgat tttcctcttc catgggagaa
    17341 taaccgctgt gcttcttgga tttcttcacg gatactggac atctcttcat ccaactgata
    17401 ggcagcttct gccgcacgct gaagtcgttc actcatttct tctggaaaaa cgccttgata
    17461 tttataattc agcgaatgct caatcgttgc ccagaaattc atcgcaagag tccgaatctg
    17521 gatttccgcc aaaattttct tctcaccgct gatcagctgt acaggatact cgatcaccag
    17581 atgataagaa cggtagccac ttgctttttt atttgtgatg taatctcgtt cttggagaac
    17641 tttcatgtct tttcttttac ggatgatctc aacaacttga tggatatctt ctacaaactg
    17701 acacatgatc cttaacccgg caatatcaga catttcttct tctaaacgtc ccattggaat
    17761 atgccgtaat tgtgattttg ttacaatact atcgactggc ttgactctcc cagtgacaaa
    17821 ttcaatcggg acatgtcgat tttgttcacg gaactgtttg cggatccccc gcagtttcac
    17881 tttcaattct gatacagttt gttcatatgg tgctaaaaat gcttcccaat ctcgttccat
    17941 agctgatacc tctacttcat taacattcat tgtttattct accatatatg caaagttttt
    18001 tcgtactctt gatttatgca ttgaccaaaa aaagaaaatt aggcaaaata gacaaaaagg
    18061 agtcgatcaa atgacacaaa tgcttgaaat cgaatataaa tctatgctga caaagcaaga
    18121 gtacgagata ttcatcgctc actatcaatt aacagataaa gatttccgtg tacagactaa
    18181 tatttatttc gataccgttg atgaacagct gaagaaacaa aattgtggcc tacggatccg
    18241 tttcgttggt catcaagctg aatatacatt aaaaacacct gctgaaaaag ggcgtctcga
    18301 aacaacggat acctttataa cagaagaagc agaagcattt atcttcgaaa aacgtctgcc
    18361 tcgttcaggc gcagtttttc aaaaactttc tgatttgaat atcgatcctg cttcattaat
    18421 ccaaaccgga aagcttacaa caaaacgagc tgaattccct attgaagaag gacttttagc
    18481 tattgatgaa agctggggag acaatctaca cgattttgaa ctggaactag aggtagggga
    18541 tgcatctgtg ggaaaaattg cttttataaa ttttttaaaa cgtttttctt taccttatcg
    18601 tcctgctaag aacaagatcc aacgtatgct tgaagctaaa aattagaaac ctttcatttt
    18661 cacaaggcac ttgagttcat ttttttactt ttttctgtta agttatagga tgtgaaagaa
    18721 tctagtcact agttgtatag aatctatttt gggggagatt catatgatcg aaatctattt
    18781 atttgttaat cctttagggg aatactgttt cgaaatggaa caacagttat tacaatttat
    18841 cgaagaagag tacgggacac catcaaaaga aaagatgcaa tttcgctttc ttcctttggt
    18901 caatttgcag acaattgggg atgtcatgca acaaaaagga atttctcaga acaatcttga
    18961 ggaacgtaat cacttattct caatgactta ttctgcagca ttggactgca aagcggcgca
    19021 gtttcaagga aagaaaaaag gccgccgttt tttgatcaaa ttgcaagaag cagtcggctg
    19081 taatgacata ccatattctc aagaacttgc tgaatccata tttgaagaaa tcggcggcga
    19141 tttgaatatg tttaaagaag atcgtcagtc tgacttcgtt aaagaagtat tcttttctga
    19201 tcaaaaaatt gctcgggaaa tgggcatcac aaaacaccct tctgcagtcg tttacaatta
    19261 tgcctgcgac cgtgattttg gtgttttagt agaagtaaaa ggtacaagta gcctttggag
    19321 aatcacagag ctgtgtaaaa caacagataa ctcatatcaa gtttttaatg gtgaacattt
    19381 acatacaaat gattctcagt tccattcaag aactagccat ttacacttgc tttctaatta
    19441 aaa
//
LOCUS       DANKKG010000045        19024 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000045 DANKKG010000000
VERSION     DANKKG010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.045"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            68..763
                     /locus_tag="PW004_001332"
     CDS             68..763
                     /locus_tag="PW004_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDL2726314.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            complement(1205..2002)
                     /locus_tag="PW004_001333"
     CDS             complement(1205..2002)
                     /locus_tag="PW004_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HDL2726315.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(1999..3183)
                     /locus_tag="PW004_001334"
     CDS             complement(1999..3183)
                     /locus_tag="PW004_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HDL2726316.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(3208..4455)
                     /locus_tag="PW004_001335"
     CDS             complement(3208..4455)
                     /locus_tag="PW004_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HDL2726317.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            4670..6415
                     /gene="dnaX"
                     /locus_tag="PW004_001336"
     CDS             4670..6415
                     /gene="dnaX"
                     /locus_tag="PW004_001336"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HDL2726318.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            6435..6749
                     /locus_tag="PW004_001337"
     CDS             6435..6749
                     /locus_tag="PW004_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HDL2726319.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            6774..7370
                     /gene="recR"
                     /locus_tag="PW004_001338"
     CDS             6774..7370
                     /gene="recR"
                     /locus_tag="PW004_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HDL2726320.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            7434..8078
                     /locus_tag="PW004_001339"
     CDS             7434..8078
                     /locus_tag="PW004_001339"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HDL2726321.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            8093..8422
                     /locus_tag="PW004_001340"
     CDS             8093..8422
                     /locus_tag="PW004_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HDL2726322.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            8436..9374
                     /gene="holB"
                     /locus_tag="PW004_001341"
     CDS             8436..9374
                     /gene="holB"
                     /locus_tag="PW004_001341"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HDL2726323.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            9410..10234
                     /locus_tag="PW004_001342"
     CDS             9410..10234
                     /locus_tag="PW004_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HDL2726324.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            10227..10574
                     /locus_tag="PW004_001343"
     CDS             10227..10574
                     /locus_tag="PW004_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HDL2726325.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            10643..11515
                     /gene="rsmI"
                     /locus_tag="PW004_001344"
     CDS             10643..11515
                     /gene="rsmI"
                     /locus_tag="PW004_001344"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HDL2726326.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            11624..12745
                     /gene="dinB"
                     /locus_tag="PW004_001345"
     CDS             11624..12745
                     /gene="dinB"
                     /locus_tag="PW004_001345"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDL2726327.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(12799..13401)
                     /gene="nrdG"
                     /locus_tag="PW004_001346"
     CDS             complement(12799..13401)
                     /gene="nrdG"
                     /locus_tag="PW004_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HDL2726328.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(13716..15905)
                     /gene="nrdD"
                     /locus_tag="PW004_001347"
     CDS             complement(13716..15905)
                     /gene="nrdD"
                     /locus_tag="PW004_001347"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HDL2726329.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            16204..16998
                     /locus_tag="PW004_001348"
     CDS             16204..16998
                     /locus_tag="PW004_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2726330.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            16988..>19024
                     /locus_tag="PW004_001349"
     CDS             16988..>19024
                     /locus_tag="PW004_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2726331.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIE"
BASE COUNT         5949 a         3469 c         3956 g         5650 t
ORIGIN      
        1 ggtacttaac aaatgttaat atcattagta aaataccttt ggactttaaa aagttttaaa
       61 gtactgtatg atctttttag atgaaagttt ggatggggga gcatcattgg atagtagagt
      121 cttaaaggaa tatttggata aaaatgattt tccgattata cgcaaaggat atcataaata
      181 tttgacattc gaagggatgg aagattccca tacgtatatc ttgaaagagg gaacggtaaa
      241 agcaagtgtt atttcaaatg atgggagaga atttaacctt cgctatatca aagatcttga
      301 aatcgtctct cttttaaaag acgaatattc acaattcatc gattcgccgt ataatattcg
      361 agttgaatca gaatatgcag agttttatcg aatcaatcgt gtgcgctttt ggcaaaatat
      421 caaccaagac ccaaagctac aacactatgt aaaagagtat taccgctatc gtctgcttta
      481 ttcgatgaaa aaaatgcagc aaatgctatt aaacggaaag ctgggagctg tttgtacgca
      541 actttacgaa ttatatgatt tattcggtgt gcggacagaa gaagggatgc tgatcgattt
      601 ttcgatcacg aatgaagaaa tcgccaaatt ttgtggcatc tcaactgcta gcagtgtaaa
      661 cagaatttta aaacaactga aaaaagatgg tattatcacc gttaaagacc atcatatcgt
      721 tattttgaat atggacttgt tagaagacaa tattatcttt tgatgaacaa agaggttgtg
      781 aaatcagcgt tttgacctga gtattagaga aacaaacgga aaaatagttc ctcatatttt
      841 gccgtttttt gagctaatac cgagggtcag ctgattgaac accgtttacc aagaggttgt
      901 gagcctgccg tttaactctg agtcacaagg aacaatttca aaaaacagct tttcatgttt
      961 gatgaatatt gtgacttgtg accgaggagt tggtaggaga acaccgttta ccaagaggtt
     1021 gtgaaaaagc tgttctgcat caagtaataa gaagagccaa gtaaaaacag tctcttatgt
     1081 ttttaacttg gctggactta tcaccgtaga tgtaagcttt tgaacgccgt ttatttgcaa
     1141 caaaaggggc tgtgacacga tttttgtcac agcccttttt ttgcgaacat tcatttagca
     1201 atttttagaa acttctgccg ccaccgccaa aagtcccgcc accgctagaa tgggtagtac
     1261 ttccgccacc gccgcttcca ggaggtgggc ttttggggat tcttcttgtc gtgacaaaag
     1321 aatttgtcag acgatcggtt ttttcagtta atttgatact ggatttttct cggaatggat
     1381 atttgtagat gcctgatttc aattgataac gcgaaacagt cacaaaataa aagacaagac
     1441 taaggataag agccaaaaca acagcaatga tgatttctac tgtcgtcaaa accttatagc
     1501 gtgtaatttt gccagtttct tcgtctacac gataatgtcc tcctggaact ccgtcttcca
     1561 catatgttgc tgatttagac aggtaagctt ttgcagcatt gtaataggca gcatttgtca
     1621 tctgatcata aacatcatct aagatcgaat cgattctgct atcagtcaaa taatcgatca
     1681 tattaccaga agtcgaaatg tagatttcac gctgtcccat atctaatagc aatactgagc
     1741 cattttgatc atttccgact gcgtctctta agtaattatc agcaaagtct cttggttctt
     1801 ctgaattaga tgtagtcgtt acgatcaaga ctcgtccttt gattttttca ttcagctttt
     1861 ctgcctgttg ttccaaagat tggatttgat ctgttgtgaa taacccagcc tcatcattta
     1921 ccgtagctgt atctgcatga gtagccaccg gccagccaac acttactagc aaaagaaaga
     1981 ataaaaaaaa ttttttcatc atatcaagta acctccgatc agaaagagga tcgctagccc
     2041 agcgaaaatc ccaagagcgg tcagtcccaa ttttttctgg ctgatcggca ggacaccgct
     2101 gacctttcct gtttgtccat tcattgcgta gtagtagact tttttacttg agtcattgct
     2161 tcgataagta acaagccata ccggcagtaa tacgtaatta tttacttcgt ttgtgattga
     2221 agcagaagtt cgcacaccgg tcaatgttgt atatccattt gcggtatctt ttaacagctt
     2281 ctctgaatag tcttttaatt cagattgaat ctgtgatttg attgcctcat attcgatatc
     2341 tcttttttct gcttggaacc cggctaaata ctggctttta aaatcaattg ctttatctaa
     2401 cgggaaaggt tgcacgcctt cgaccatctt ttgttgcgta ttttttgata aggcattttt
     2461 tactagttct ttaaaagaaa gcttgcctgc tcttgaaaca gcaaattgct tcgtttctgt
     2521 gtattcgatg tcacctatcc gccatatgcg gataactgta ccattcgcct gcatggcacc
     2581 atctacttca gcatctgtag cccaataagg gaaatacaca cctgtcaact tatcgatttg
     2641 atccttattg aaaaaagctt tgggaacaaa ccattttttc ttggtccaag cgagaaatct
     2701 gttgattgct tcttctttct cgacagcaaa aggaagtact ttatttggca agaattctcc
     2761 gcttaaacgt cccgatagta cgacgggatt atggcagtag taacaataag tcgcagcagt
     2821 tgtcgcatca gtcacgatct ctgcaccaca gttgggacaa agaaaaagat ctatcgctgc
     2881 ggtattatct gcagattcgt ccgctgtatc tgaaaaaccg cctgcctcgt caaccgcttt
     2941 gcgctcggct tcattcatat cggcaagctg ttcttgggct gtaaaagtaa aatcttcggt
     3001 aggtgaaggt tcttctttgc tttcttcgcc agccatacgc gcttcttttt gcttttgctc
     3061 atattggctg acttcttctt ctgtatagat attcaggcag tattcgcaat gaaatttttg
     3121 gtctttggga tcaaaagtca acggaccgcc gcaattagga catttatgtg taagaacatc
     3181 catcagctgc acacctttct aaataaatta attaacgggg attcctttaa acggtttacc
     3241 acagtttgga cagaatttag ggatgccgtt ggaaagatcc acgatctcat tgcattcact
     3301 gcatcgcatt tgtagcttag gctgaacgtt ttctacaggt ttggcagctc cgcagttgga
     3361 acagaatttt ccggaatttt ctgtcccgca aactggacat gtccaagtat ccgcatttcc
     3421 ttgtgcagtt tgttgatttt gtttttctgc ttgttgtttg atttgttctt ggttattttg
     3481 agctgcttga gaaagataag aaccatttgc attcattccc atacctacac ccataaagcc
     3541 ggtcattgct cctgcttcat tttttccggc tgcttccata ccacgtgcaa tggaaccttg
     3601 cacgtagcct tcacgtacgc taggatcacc tagcatcgcc ccttcgttac gcatattgat
     3661 cagtttcaca gaatcatcgg tgtaagaaat gctggctaca gccacagaaa caatctccat
     3721 cccacgtaat tcacgccaag aatcatccaa tgcagtatcc atgtatttgc ttaattccaa
     3781 actcttagat ggtacataag aaatccgttg accatctgca gacatctgat tgattgctga
     3841 ttgtagcgct gttaggaatt cagctaaata ctgctcattg atatcattga tttccacttg
     3901 tgttttgttt ttagggattg cgtttgtata aaataaaatc gggtcaacga catgaataga
     3961 ataagtgcca tgtgcccgta agaataattc tgcgttatag aaattgtcaa agtagttcaa
     4021 cggcgcgctt gtaccgaatt tgattccctt tatttcttgg agattgatat aaaaaacttg
     4081 ttgtttctgc ggtgtgacgc cgccaaattt gaatcggtca aaagtttcag caattgcttc
     4141 tttcagtgtt ccattgaaca tggaaggagc tgcgtcattt ttaatggtgt aataaccttc
     4201 ctcagcggta taatcgatga tttttccacc atcgaccaaa agcatcatca tgtttggata
     4261 gacatggacg actgttccgt ctgttaaaac atcttctgtt cctttacgat tggagccacg
     4321 tttgtcatct ttgcggactt tgacaccttt tgtcatgacc gttgtatcac tcatattgtc
     4381 tggttcgatg acttctagcc attgatctgc cagaccgccg ccaaccatac ttgttgctgc
     4441 tttgataaga cccataaaaa attcctcctc tgattcagct agtgccaaac aattattgtt
     4501 tatttattat accataggaa gatgtgaaac ttctcggact agagacggaa ttgaaatgga
     4561 aaattgcgta ttgtttaaat acctgaaaaa gccgagaagg atagtagttt ttttacctaa
     4621 tatcacgtat aataagagat agtgaatttt ttaaaaaagg gggatcctta tggcatatca
     4681 agcactttat cgagtctggc gttcgcagcg tttcgaagac atcgttggac aaaaagctgt
     4741 gacacagaca ctaaaaaatg ctattgtttc ccacaaaacc tcacatgcct acttgtttac
     4801 aggacccaga ggaaccggaa agacgagtgc agcaaagatt ttcgcaaaag caatcaactg
     4861 tccgaacagt aaagacggtg agccatgcaa cgaatgcgaa atgtgtcgat caattactgc
     4921 tggtacgcaa gaagatgtga tcgaaatcga tgctgccagc aataacggag tcgaagaaat
     4981 ccgatttatc cgtgatcggg caaattatgc gcctacaaag gcagcgtaca aagtgtacat
     5041 tattgatgag gtacatatgc tttcaacagg agctttcaat gccttgttga aaacattgga
     5101 agagccaaaa gaaagtgtca tattcatctt agcaacgacc gaacctcaca aaataccagc
     5161 tacgatcatt tcacggactc agcgatttga ttttaaacgg atcaatacgg cagatattgt
     5221 cgaacatctt gaatttatct tgaaagaatc gactatcagc tatgaaccag ctgcattgaa
     5281 tgtgattgcc cgttctgcag aaggcgggat gcgggatgca ttgagtattc ttgatcaggc
     5341 aatctcgttt agtgaaggaa caattacttt agatgatgcc atgcaagtga caggtagctt
     5401 aactgacgaa atgatggaca gttacttgag tgcttgcgtg aaaaaagaag taccagaagc
     5461 attggaaaca ttgaaccaaa ttctcgcagc cggcaaagaa tcacgtcgct ttctagagga
     5521 tatgttacaa tactgccgtg acttgctgat gtatcagcaa gcacctaacc tagttgagga
     5581 acaatcgagt acgatcagaa gtacatttac agaattggca caaactgttt cagcggatca
     5641 gctgttttca atgataaaga ttttgagcga tactcaaaat gaaattcgtt ttaccaatag
     5701 tgccgctatt tatttggaag tcgctacagt caaacttgca cagctaccgt ctgcttctgt
     5761 tccttcagca gatgtagcat ttgcagatga tcagatagta aaacagctac aacaagagat
     5821 taaggaacta agaaacgaaa tcaaggaact acgcagtaat acaggaattg agaatacaaa
     5881 agtacagccg gtacagcaaa caaatcataa aaaacagaca agtacctatc gtgtaccgac
     5941 ggaacgagta tataaagttc tgcgggaagc gacaagacag catctgaacc aagtaaaaga
     6001 agtatgggaa gacttgctga tgagtctatc tgtcactcag cgagcaatgc ttaaagcaag
     6061 cgaaccagtt gcagctggac ctacaggact agtgattgcg tttgattatg agattgtttg
     6121 tcaaagagca gcaaatgacg aagaattgca gctttcggta cacaacaact taagccgaat
     6181 gatcaaagat tatgcgcctg atcctgttta tattacaaga gaaagctggc cagaacttcg
     6241 aagaaattat ttgacgcagg cacaagaaga gggaacagaa aactcttttg cttctgaaga
     6301 ctcagaagga gaaatcgagt tgcttcctca agaacaagaa gtaccagaag aaacacttgt
     6361 agtaaaagca gaggaactgt ttggctccga agctgttaca gtggtagatg attaataaaa
     6421 aggagtagat aattatgatg cgtggaatgg gcaatatgca aggcatgatg aaacaagtac
     6481 aaaaaatgca aaaagaaatg gcagaagcac aagagctgct aaataaaaca gagtttacag
     6541 gtaaagccac caatgaattg gtcaaagcgg tttttacagg cgatcgtcgc atgaaagata
     6601 tccaaatcaa tgaagaagtc gttgatcctg atgatatcga gatgcttcag gatcttgtcg
     6661 taatggcagt caatgatgcg atcgcacaaa ttgatgtgga aactgagaaa aaattaggga
     6721 aatacgcaaa aggtttgcca ggattttagt tttaaactga aggagtaatg aatatgcaat
     6781 atccagaacc aattgcgaaa ctgatcgaca gctacatgaa acttccagga atcggtcaga
     6841 aaacagcgac aagactcgcc ttttatacga tcgatatgaa agatgaagta gtaaatgaat
     6901 ttgctaaatc tttgttaagt gtaaaacgcg atttgcattt ttgtagtatc tgcggaaaca
     6961 tcacagaaga agatccctgc gaaatatgta gaaatccttc acgtgatcga agcgttatcc
     7021 tagtggtcga agagccgaaa gatgtaatgg caatggaaaa aatgcgagaa tatcacggac
     7081 tttatcatgt tctccatggt gtgttatcac cgatggaagg gacaggtcca gaagacatca
     7141 atatttcttc tctgttgcag cggctgcatg atgcagatgt aaaagaagtg attatcgcaa
     7201 caaatgcaac aacagaggga gaagcaacag ctatgtatct ctcacgtctg atcaaacccg
     7261 caggtatcaa agtgactcgt ctagcacatg gtctgtctgt aggctctgat atcgagtatg
     7321 cagatgaagt tactttattg aaagctgtcg aaggaagacg tgagttataa atttctaatt
     7381 aagtgttttt tcagatacaa tataaaagca gcacaagaga tgggggcaac aatgtgaacg
     7441 gactatttat tacaatcgag gggcctgatg gtgcaggaaa aaccagcatc ttgaatgaac
     7501 tttaccctct tttgaaagaa gtagcaaaaa cagaaatcat tcaaacaaga gaaccaggag
     7561 gcattccgat tgctgaagag atccgagcag tgatccttga tccaaaaaat gatcggatgg
     7621 atgaacgtac ggaagcactg ctttatgcag cagcaagaag acaacatcta gtcgaaaagg
     7681 ttctgcctgc tctggcaaaa gggaaaatcg ttttatgcga tagatttgtc gacagttctc
     7741 ttgcttatca aggtgcaggc cgacgcatcg gcgttaccga gattgctcgg ttgaatgagt
     7801 ttgctacaga aggtactacg ccggatttta ccctttatct tgacgtagat tcagacaccg
     7861 gattaagaag aatcaagaaa aacagacaaa atcaaatcga ccgtttagat tcggaaggtt
     7921 tggaattcca ccagcgtgta cgtcatgctt acttaaagct ggcagaagag aatccggaaa
     7981 gaatccacaa agttgatgca agaaaaagct ttgaagaagt gctgcaaaca agttatcata
     8041 caattattga acagtatccg caattttttg aaaactagga aggtgaacgt ttatgaaaat
     8101 tattttagcg attatccaag acaaagacag caaccgttta tcaaatgaat tgattgatgc
     8161 aaatatccgt gccacaaaac tatcatcgac aggcggcttc cttaaggctg gaaacagtac
     8221 gtttatcgtg ggtattgaag atgaccgtgt agaagaagca ttggaaatca tcaaaaaaac
     8281 gtgtgaatca agaaagcaat ttgtatctac acctgttaca ctggatattt cgatggatgg
     8341 gggcgtccct tatccagtag aagtagaagt aggcggtgcc acagtcttcg tcttacctgt
     8401 tgaaggattc catcaatttt aggaggagct gagctatgaa tcaagaaata aacttggctc
     8461 aaatgcaacc gatcgtgtat cagcagcttc aacggagttt tgagcatggg cgtatcgctc
     8521 atgcttatct ttttgaaggg gagaaaggaa caggaaaaca tgaagtaggt atatggcttg
     8581 cacagcacct gttctgtacc caaatgaaag acaatctgcc ttgtggaaaa tgcaacaatt
     8641 gtcagcggat cgaaaagaaa gaacatccag atgttttggt tattgaaccg gaaggacaaa
     8701 ccatcaaagt cgatcagatc agacgtttgc agacggaatt cagtcgcagc ggttacgaat
     8761 caagaaaaaa agtttttttg atcaaagaag ctgaaaaaat gaatagcagt gcggcaaaca
     8821 gtttgttgaa attcttggaa gagccccctg gagatttttt agcggtgtta gaaacagatg
     8881 cagtaggaag aattctccct actattcaat ctcgttgtca agttctgcat tttcaagaac
     8941 tttcgaaaga agcactgatc catcgattgc aagaagagca gattccattg gaaaaagcaa
     9001 aacttttggc tttcttaaca aacagtttag gaaaagcagt tgaaatctct caagatgaat
     9061 ggtttaatga tgctaaggac ttgatttatc aatggttcgt ttacttgcaa aagcaagata
     9121 ctcaagcctt tatctatgtc caaaaaaaat tggtaaaatc atttaaagaa aaaacacaac
     9181 agtttacagg attatctgtc ttgatgtttt attatcagga agcacagcgt caagcagtag
     9241 caaacggcga atatggcaaa gtgaaacaga taaaccagat aattgaacgg atattgatcg
     9301 cagagcagaa actacgcagc aatgtcagtt ttcaggctgt tgcggaacag ttcgttttgc
     9361 atacgatttt ttagcttgcc gcaaaggatg atgatcaagg aggatggaaa tggtagaagt
     9421 agtaggtgtc cgatatagag atgcaggtca tatctattat tttgcaccag gagactctga
     9481 atatgtttat agtgacaagg tattagtcga atctcagcaa tccttacata ttgctactgt
     9541 tgtccttcct aaaaaagaga ttgatcccaa tgaccttcct gaagctatcc gtccgatctt
     9601 gcataaagcg tcacaagctg aattagaaaa agacaaaaaa aacaaaaaag atgcttatga
     9661 tgcattgaaa atcgcaaaag aaaaagtgca gttgcgtgag ttggaaatga aaatggtcaa
     9721 agccgaatac acatttgatc gaaataaatt gattttttct ttcacagcgg acgggcgaat
     9781 tgactttaga gaattggtaa aagatctggc cgctgtattt cgtactcgta ttgagcttcg
     9841 acaaattggt gtccgcgatg aagcaaagat aataggcgga attggtccat gtgggcgtcc
     9901 attatgctgt tcttcttttt taggcgattt cgtgcctgta tcaatcaaaa tggcaaaaga
     9961 tcaaagttta tctttgaatc caacaaaaat ttccggttta tgcggacgct tgatgtgctg
    10021 tctgcaatat gagagcgaag aatacgagac agcgaaaaaa gaactccctg actttggaaa
    10081 agtaattgct acacctgatg gtaaagggaa agtcgtaggg ttgaatctgc ttagccgtat
    10141 cgttagtatt cggctgactg gacgggaaaa tcctgtcgaa tatgaatggg atgaattgaa
    10201 agaatttatc caagtaggtg agatgaatgg ataagagatc attatacgac ggattgaatc
    10261 aattagaatc agagttgaga aatacattaa cagaacttgt tgaaatgaag catgctttgc
    10321 atgagatcgt agaaaaaaat acaactttgg agatggaaaa tcaacgtctg cgggaacacc
    10381 tgcgggaatt gaaccaagtt gcacaagctc cagctgaaaa tgtgaagcag gaactttcta
    10441 agtctcgtat gaatcttgaa aaaatttatg aagaaggatt ccatgtctgc tacgatttat
    10501 atggatcaag aagggacaat gacgagcctt gtgcgttttg tctagaagtg atctatcgcg
    10561 aaagcggtcg atgaccaata acaagaggag actggagcgg agaaatactg ctccagtctt
    10621 tttattaagg agattgtgac agatgtataa tcaaaaaagt ttcaagggaa atccggtata
    10681 tggggattta tatcttgtac cgacgccaat tggaaattta gaagatatga gctacagaag
    10741 tgttcgtatg ctgcaagaat ccgatcttat cgcaagtgag gatacgcgta atacacaaaa
    10801 gttattgaat cattttgaaa tccatacacc ccaaaaaagt ttacatgaac ataattataa
    10861 agaacgtgtc cctcaattga ttgctttgtt aaaagaagga aaaacgattg ctcaagtcag
    10921 tgacgcagga atgccttcca tcagcgatcc tggacatgag ctagttttag cctgcataaa
    10981 agaagggatt tctgtgatct ctatcccagg acctacagct gggatgacgg ctttgattgc
    11041 ttcagggcta gtgcctcaac catttttatt ttatggcttc cttccacgta agaaaaaaga
    11101 acagaaagaa acattgaaaa agctaaaaga acagacagtt acgctgattt tttatgagtc
    11161 accttatcgg atcactgcta cattgacgaa tttcttagaa atttttggaa atcgccaagt
    11221 cgttttgtgt cgggaattga caaaggtcca tgaagaatac cttagaggaa gtacggaaga
    11281 gctactcgct tatatcgaag aacatccagt aaagggtgaa tgttgtctgc ttgtagaagg
    11341 gaatttgttc gaaatgccag tgacggaagt tgatgaagag ttgggaacgt taaaggaaca
    11401 agtagaagca aagatcgctt caggcgaaaa accaaatgct gctatcaaag cagtagcgct
    11461 gaaaaatggg ctgaaaaaac aagaagtcta ccgtcagtac catgaattag actgagatag
    11521 tataagttgt ctataatgaa agactaatga ggaaatcagc tactaacttt tcaaatctct
    11581 tgaacgcgaa cgaatgttct tgtataatga ataagaggtg aggatgatgg agctgcaatt
    11641 tcctttacat aatgatacat caagaaaaat cattcatatt gatatggatg cttttttcgc
    11701 ttcggttgaa gaacgagatc atcctgagtt tcgcggacat ccattagtaa ttgccagaca
    11761 ccccagtgac actggtgggc gtggagtagt gactacagca aattatgaag caagaaaatt
    11821 cggaattcat tcagcaatga gtgcacaaaa agcgtatgag ctttgtccgc aagccatctt
    11881 taagcctggc gaccatcata aatacagcga tatttccaaa caagtacggg aagtattcta
    11941 tcgttataca gatattgtgg aaccagttag tatcgatgaa gcgtatcttg atgtgaccga
    12001 aaataaaatc aattgcaagt cagccatcaa aatcgcccga atgatccaag cagatatttg
    12061 ggagagtgtc cagctaacat gttctgcggg agtaagttac aataagtttt tggccaaatt
    12121 agcatcagac tatcaaaaac ctagaggaat cacggtagtt gcccctcaag acgctgtgcc
    12181 atttttaaaa gctttgccaa ttgaaaaatt tcatggggta gggaagaaaa ctgtacctag
    12241 aatgcatgag ctagggattt atacaggaaa agacctatat gaatgcacag aaatgatgtt
    12301 gattcgaaat tttggaaaaa tgggctattc tctttatcgt aaagtcagag gaatccatga
    12361 ttctccagta aacgttacaa gagagcgaaa atctgtggga aaagagcata cttatgggca
    12421 gccgttgcaa acggaagaag cagtgctgac tcagttgcgc caactagctg aaaaagtaga
    12481 agaagcactg agaagagtcc aaaaacatgg gaaaacagta gttttgaagg tcaggtacac
    12541 ggactattct actgtgacca aaagagtgac attgccagaa tatatctata aaaaagaagc
    12601 actcttttat caagcaagcc ttatatggga ggaaatcttg ggagtagaaa aaggaatccg
    12661 attgttaggg attactttga cgaatctgga cccgatgact tatgaaaaca tcgtccttcc
    12721 attatgggaa aatcaagaaa tataaaaaca gctttgagag ttgaaaccgg aaatccgttt
    12781 tctctcccaa agctgttttt atatcaagtc ttgctttttg atttgttcat aggtttctgt
    12841 tgcatccgta catttttccc aaatcacaac ttgattggat tctaaagatt tttggacgtc
    12901 aatgatccgc tggttgctac ttccgcgaaa ttgtagtttt aggtcacgtt tgcttaattc
    12961 aaagcgtccg tcaacaagga tatcgatttg cgaaaggagt tctaatttat cttctgaatc
    13021 ttgtagcaat tcatcaaagg tatagcctgt ccatgaccag atatcttttg tcgtaccgaa
    13081 ctcttttcgg atacgtcgta cgacttgcag acaaacatct gtattcaaaa aaggttcgcc
    13141 accaagtaaa gttaatcctt gcacgtaatc gttgcgcaag tcagcgatga tttgttcttc
    13201 taattcaggt gtgaatggtt tgccatatcg aaaattttgt gcagctttgt tgaagcagcc
    13261 ttcgcaggca aataagcagc cactaacgta aagactgttg cggacacctt caccatcgac
    13321 aaaattaaac ggtttgtagt cagcaatata gttttggctg tagtcttttg ctaaccattc
    13381 ttttggtttt ggatttctca taaaaaaact tctttcattt aaggttcaaa gatatagcta
    13441 ttgaagataa aatctactcg accacataaa taagaacaaa ctgacctaaa tccattgttc
    13501 aagaaagctt gttcttattt tgatagaggg cgttcaaaga tacaaagcca tcgaaagagg
    13561 gtggtgaaaa aagacttgtc atagcctctt attctgaata aacggtgttc aaaagcttgc
    13621 atctgcagta ataagcccaa ctaaacgaaa aatatgagga actatttttg tttagctatt
    13681 cttattactt gatgcaggac agctttttca caacctcatt tcatgtgttt cacacgtgag
    13741 gaaatttctt tatggcggcc atgtaccatc ggacgagctt gcggatttcc taaatagcca
    13801 caagtacgtt tgactacatc acatgttttt ggatcatgat ttccacattc tggacattcg
    13861 aaccctcgtt ctgtcggttt gaaatcacct tcgaaaccac attgatagca atgatcgatt
    13921 ggcgtattcg taccaagata cccgattttg tcatatgcaa aatcccatac agcttctaaa
    13981 gctttcggat tttgttggag catagggtat tcacagtagt ggatgaatcc acctgaacaa
    14041 tattttggat aatccttctc aaattccaat ttttcaaatg gcgttgggtt tttacgcaca
    14101 tcatagtgga aactgtttgt ataatattct ttatctgtga tattttcgat gatgccgaat
    14161 ttttctgtat ccaaacggca gaaacggtca gttagacttt cacttggagt tgaatagaca
    14221 ctgaaatgat aaccatattc atccccccat gcatctgcat tagcttttaa ttctttgacg
    14281 atatcaagtg tgaaagcttt tgcttctggg tttgtttccc attcgccacc gaagaaagct
    14341 gaagcaactt catacaatcc aatgtagcct aacgatacag tagctcgacg gtttttgaat
    14401 aattcattga cagaatcggt acgtgaaaga cgtttcccga aagcaccata catatataaa
    14461 attggcgcat ttgctggaat ggcttcttta caacgttcta cacgatagag aagcgcatct
    14521 ttcatgattt ctaagcggtc agacagtaac tgccagaatt tattctgatc tccttttgat
    14581 tcgatcgcga ttcttggcaa attcaatgta acaacaccta aattcattcg tccgacattt
    14641 acttcttgac cattttcgtc tttccaccct tgcaagaatg aacgacagcc cataggtact
    14701 ttgaaacttc cagttaattc aacgatttta tcgtaattca gaatatcagg atacattctt
    14761 ttcgttgcac attccaatgc tagttgtttg atgtcgtaat taggatcttc agggtttaag
    14821 ttcacacctt ttttcaaagt gaagatcagt ttagggaaga tagctgtccg cttttcactc
    14881 cctaatccat tgattcgaat ctgtaaaatt gctttttgaa tttctctttc aaaccagttt
    14941 tctcctagtc caaatcctaa tgaagtgaaa ggagtttgtc catttgaagt aaataaggta
    15001 ttgatttcat attccaaact ttgcatagca tcgaagatat cttttttggt ttttgctttt
    15061 gcatattctt tttggcgttc tggcgaatcg atccattctt ccgcgtcttt tagatgtttt
    15121 ttatagttca attctgcaaa aggagcaagc aattcatctg ttcggtcggc agaacagcca
    15181 ccatattgac ttgacgctac attggcaata atctgtgaga tttgtgctgt ggcggtctgg
    15241 atagattttg gcgattcgac ttcggcattg ccgattttga acccattgtt cagcatgcct
    15301 ttgaaatcaa tcaagcagca attcgtcatc ggtgtataag gatgataatc caaatcatgg
    15361 taatggatat cgcctttttg atgcgcattt gctatatgtg gtggcaacat tttcagtccg
    15421 attgattttc caacgatccc ggctgtcaga tctcgctgtg tattgaaaac attactgtct
    15481 ttatttgcgt tttcgttcac gacagtttgg tccttgttga ttagcttttc aatcgaaaag
    15541 ttgatatctg ttgctttact gcgagcaaaa tctcgtttgg tacgataatt gatgtactct
    15601 tctgctaatt catattcgtt tttttctagt aatatatgtt ccacgatgtt ttgaatctca
    15661 tagattttga cattttcagc aaaccggctt ttgatttctt ggacaaccaa gtctgcaatc
    15721 atttgaattt tttcataagt aagtggtgat aatgagccgt gaattttctt ttctgctttg
    15781 atcagtgctt cattgatctt ttgttcatcg aaatcaacat ggcggccatc gcgtttgatg
    15841 actttcatct gggatgtctc tggctgctga gtaacgacag ttttttcttc ataatttact
    15901 ttcattatct tcaactcctg ctaaattgtt cttgtctatc ataaaacaaa atcggggaag
    15961 gagcaactaa atatcggcta tatatagtat tcaatgtgct gtataattgg atttctatac
    16021 tatatgtagt gtatatttta ttgtgaaaaa aggtgaaaac ttgtagctgg tggatttgtt
    16081 ttttgcatat ttaattttgc tgaataaaat aattgaataa aattttttat tcagtccaag
    16141 taaaagtgac aaaaacataa gagcaaatta ttcgtagata tgctactatg caaataaata
    16201 gaaatgaaaa atgaaagagg aggagaagaa ttggaaaaga tgatcgaagt gaaacatcta
    16261 agtaaaagct acggcagcaa actttctccc gtcaaagcct tagataatat ctcattcaca
    16321 gtagaagaag gcgagtttgt tgggattatg ggcccaagtg gtgcaggaaa gacaacgctg
    16381 atgaatatct tgtccacgat caatttgcct acgatgggca gtgtctcgat ccaaggaaaa
    16441 gaaatcacaa aaatgaaaaa cagtgagctc agtgactttc gtcgaaaaaa actaggattc
    16501 atttttcaag agttcaattt gatcgatacc ctaaatgcaa aagacaatat cctattgccc
    16561 ctagcagttg aacggatgac taaagaggaa atggaaaaga gagtaagaca tgtagctcaa
    16621 ttgttgaata tagaagaact gctcaaacgg tatcccgatg aactttctgt cggacagaga
    16681 caacgaattg cagctgctag agcacttgtt gttcagccac aagtgatttt tgcagatgaa
    16741 ccaactggct ctttggactc gaagtcggct acagaattgt tgaactactt gaagaccatg
    16801 aatcaaaaag aaaaagcaac gatactgtta gtcacccatg atccatatac agcaagctac
    16861 tgcgatagaa ttttatttat taaagatggt gtgatttttt ctgaagttgt cagaagaggg
    16921 acaagaagag agttctttga gaaagtaata gacatgcagg cgacgattgg cggaggagga
    16981 aaaatgaatg ctgtataaac tagccgttaa aagttttttt cggcagtcac gaggctatct
    17041 tgtttatttt ttcagtttga ctttatccgt aatgatctat tattccttca gcgcaatgac
    17101 ctacgatcaa tctttagtca gaagagcaag tcaagatact tcgatagatg gtggattgag
    17161 ttttggtgga tttatcatta ctgttgtttt gctttttttc gtattcagtg ccaaccgatt
    17221 tttcttgaat agaaggcaaa aggaaatagg catctatcag ctttttggta tgaataaact
    17281 gcagatatca ggtatatatg tgttagaaat catgataatt ggattgttcg cctgtatttc
    17341 tgggatatta ctcggtatta ttttttccaa actcttttca atgattttgg ttcgaatgat
    17401 ggatatggat cttactagtc cattttttat ctccattcca tcagtagtag atactttatt
    17461 tgcttttttc atcatccttt tagcagtatc tgtatattca atatggaaaa tatggcgcta
    17521 tccaatgatt cgaagttttg gagaaaaaga acaagcagat agttcaacaa tgcgcttcag
    17581 gacaaggcac cgattactcg cagtcatcgg gttactactt ttaacgagcg gatattttgg
    17641 agctagtcat tttagggaaa tcacttcatg gatgatcagt tatcattatc ttgatacatt
    17701 agttttcttt tttccttttt taattttttt cgtttgtgtg gtcggtactt atttgttttt
    17761 ctgttataca tttcggttat ttttgatcat attgagtaaa tggaagattt attactatgg
    17821 tatcaatttt ttgataatgg gtaacacgca agttcatttg ctcaaaagtt ggcgtacaaa
    17881 ttctctgatt acagtcattc tcggaatttc tttaatgaca attggcggca cggctagtat
    17941 ctctactatt ttatcctacc aggagaaagt ttcctccccg atggactatc agtttgatgt
    18001 tgagacagag aaaaaaatca agccgattct agcagaggaa gggcagaaga taactaaaga
    18061 aatcaccttg cattataaag tcattgggag tatctatagt caaaatctcg aagaacctat
    18121 gtttcaaact gctaatctga tcacggaaac acaatatcaa gccttcagaa aagttaatcc
    18181 agatttgccg aacatttctt tgaaatcacc tggtcatact gttttgcttg atcaaatgca
    18241 aactcttttt cggaatattt cattatatgg atctagtatt ggactgccag ataatcaaga
    18301 attgaagatt caacaaatgc ttccaagtgt attaggtgac gaaacaatgg tatatctaag
    18361 tcctgtactg attgtagatg ataccgtttt tgaaaaagca aaaggtgtgg attatcaagt
    18421 agtcaatgta gatgtatcag gtggaaataa tgaaaaaata gatgaacgac tgagcaatga
    18481 cctgtcgata aagtggctca atgctgttta ttattcttat gatatcgtaa atggaagtct
    18541 acaaggagaa attagcacaa agaaacttcc ggatgaccaa atgaaggaag cacaatttag
    18601 ccaagaaagc tctcgtttga atttctcttc cagctattcg aaattgcgtg cagttaatcg
    18661 gcgaatcggt atcaatattt ttgtcacact atttgtcggg atgatcgtga ttattgctac
    18721 tggtagtatt ttgacagtcc ggcaattagc agaagcagag gaagaaaaag aaaattatca
    18781 cctattgact aagctgggga ttccacaaag aaggatcaga cggatgatct tcgagcaaaa
    18841 cgcacttacc ttctttccac ccatgatatt agggatcact cacgcagtat ttgcaatcaa
    18901 tgtcttcact cagtatataa aaactgccga ttactggttg gcttatcttg tgagtggctt
    18961 attgatcctt atctatcttt tgctttatgt cattacgagt cagttatatt gtcggattat
    19021 cgag
//
LOCUS       DANKKG010000046        18973 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000046 DANKKG010000000
VERSION     DANKKG010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18973
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.046"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(45..2189)
                     /locus_tag="PW004_001350"
     CDS             complement(45..2189)
                     /locus_tag="PW004_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDL2726332.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(2546..3919)
                     /locus_tag="PW004_001351"
     CDS             complement(2546..3919)
                     /locus_tag="PW004_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2726333.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(3919..4314)
                     /gene="rbsD"
                     /locus_tag="PW004_001352"
     CDS             complement(3919..4314)
                     /gene="rbsD"
                     /locus_tag="PW004_001352"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HDL2726334.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(4342..5259)
                     /gene="rbsK"
                     /locus_tag="PW004_001353"
     CDS             complement(4342..5259)
                     /gene="rbsK"
                     /locus_tag="PW004_001353"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDL2726335.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(5367..6380)
                     /locus_tag="PW004_001354"
     CDS             complement(5367..6380)
                     /locus_tag="PW004_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDL2726336.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(6461..7060)
                     /locus_tag="PW004_001355"
     CDS             complement(6461..7060)
                     /locus_tag="PW004_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL2726337.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            7246..7947
                     /locus_tag="PW004_001356"
     CDS             7246..7947
                     /locus_tag="PW004_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2726338.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            7957..10668
                     /locus_tag="PW004_001357"
     CDS             7957..10668
                     /locus_tag="PW004_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2726339.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            11119..12747
                     /gene="treC"
                     /locus_tag="PW004_001358"
     CDS             11119..12747
                     /gene="treC"
                     /locus_tag="PW004_001358"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HDL2726340.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(12807..13988)
                     /locus_tag="PW004_001359"
     CDS             complement(12807..13988)
                     /locus_tag="PW004_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL2726341.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            14240..15763
                     /locus_tag="PW004_001360"
     CDS             14240..15763
                     /locus_tag="PW004_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HDL2726342.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(15832..16560)
                     /locus_tag="PW004_001361"
     CDS             complement(15832..16560)
                     /locus_tag="PW004_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2726343.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(16573..17853)
                     /locus_tag="PW004_001362"
     CDS             complement(16573..17853)
                     /locus_tag="PW004_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDL2726344.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(17994..18596)
                     /locus_tag="PW004_001363"
     CDS             complement(17994..18596)
                     /locus_tag="PW004_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HDL2726345.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
BASE COUNT         6133 a         3478 c         3719 g         5643 t
ORIGIN      
        1 taaaaaggga gtgtgacagt gtcacatccc ctttttaatt tattctattt ctttaaaggc
       61 agatcaagcc agaatgattt gtctaaactt aacaaggtta gtacaggaac agttgcagct
      121 ttaactgtac gagtagttgt ttctggtatc tgtcttgccg taaaattgtt ccacaaatcc
      181 cagattgaag cgacacggaa gatggaagaa gcgttgttga cgcgttttat cgttgaaaat
      241 tctaatcctg tgattggcac gccatctgtt gtccatttga ttggcacatc gaatcgctta
      301 gacatattga ggatttcagc gtttgcttct tcagttgttg ctgcttcttt tttcggttca
      361 tcatattttg tcaggtcgta cgcttcgatg atcgcgttca atttttgtga atattggcga
      421 tctgtagcat aacgtccttc caaatatttg gttgcttctt tgtagtcttt tgtttccgac
      481 ttccaagttg gtttgtagaa atcaggattg ccagtcagtc catttttgat caagtcggca
      541 tagtcttcca aactttcctt gtaagaagga tatttacgga attgtgcgcg gatttggtag
      601 ctttgtccgg aactatcttg ttcaagtgta ttgaaggtca cgctgcttcc tttatatgat
      661 cctttgatac caaaaaggtt atgatttggt tcgcttgata ataagctgtt tccagatcca
      721 ctttcaagga tagcttgagc catcatgact gatgcgtaga ttccttcttt ttgcccgatt
      781 tcctgtgcat catctgcaat ctttttcaag aactgatcag ttgtttggtt ttgactgaaa
      841 tggaacgagc catctaatcc atctaaaata gatgagccgc ctgtcagcga acctgaagca
      901 gcaggtaaac ctgctaagtt tactcggaca gcaaaatcag gcgtgaaatc tgaaatagaa
      961 gttactcgaa ctttatctcc tggttgaggc gcatggataa attgtccgtt cccaatatag
     1021 atacctacat ggtgagtaac accggaagaa ggtccccaga agacgaggtc gccaggttgg
     1081 gcctgactta ctggaatctg cgtacctgca tattgctgtt cgcctgtcca gccaccgata
     1141 taatgaccgg ttgctttcat atatacgtaa taagtcaaac cagaacagtc aaaagtattt
     1201 ggaccttttg ctccccatac atatggtttt ccaagatgct tgaatgcttc tcgaacgatt
     1261 gcttgttgga cattcgttgt atttgtaggc aatacagcat caattggtga ttttacaaca
     1321 aaatcagaag aagcagaaga tggattctct ataccgcttt gatttgctgt atttggacga
     1381 gatgaagcat tgttcgttcc gccattcgat gaaccattgt ttggatggtt tggttctctc
     1441 tgagtatttc cattgttatt cgatccagat gggtttgttt gattcgaaga attcccgctg
     1501 ttcgaattat tgttattatt gtttgaatta tttgaaccat tattattgtt gttattaccc
     1561 acatctggtt tgtttggcgt ttccggatct ggtgtactag ggacttcttc actaggatca
     1621 gttggtttct ctgttgaagt atccggtcca gctttaaatt ctgcagtaat agataaattg
     1681 ttcgtaccta ctgtaaaggt ctttgcatca taatttactt ctttggcacc attaacagcg
     1741 actgaagcaa tttgttcacc ttcagctggc gtaatagtgt aagttacctt cgtttccgct
     1801 aataaacctt ctaccgttcc actttccgta atatccaaag gttgctgttc aggggaatcc
     1861 gctgtcgaag cagtcagttg gatcttacca atcaaagaag gagcaattgt aagggaatat
     1921 gtctcggctg gcttttcatt ttctgatgga tcagccggat caatttcagt tggctcggtc
     1981 gatcctgttg ttgagttagt tgttccagtt gggtcttctg taaaagaatc cgttgtttca
     2041 ctagtagctg agctggatgt agtcgtatct gttgtgactg tttgatcagc tatttgatta
     2101 tctacggcaa atacggtatt ggtcgacaaa acagacggag caatcagcca agtagcagct
     2161 agcaaacgta tcgttttttt atttttcata ataaactagt ccttttcctt tcattgattt
     2221 tctgtcttat tcagcattca ttagacattt tttggaacag ataatcgtgt cgaaaagctt
     2281 tttcttttca taaatctgtc tgtgtattct aaataaaaca atttgtaaat aagcaacgag
     2341 cattcattcg tttttctttg acgttcattt tattgcatag accacctttg tttggggata
     2401 aacaagtggg gataaaaatg gttatgcata tgtaaattat accaaagttt ggaaagtatc
     2461 acaaaaattt ataaaaataa tgatcctaaa aagaaaaaga ctgaaatatg tgataaacat
     2521 ctcatacttc agtctttttc attaactatt cataagacat taggtcttca tcaaacccgc
     2581 taacgagagt agtgatcatt gctacgacta gactaacagc cagtcctgct aagtaccaga
     2641 aaaaagtacc ggaaatgaat gcaggcaatg tggtcagact accaaataca aaagcgatcg
     2701 ctttcacttg ggcaaatccc attacagctc caccagcagc tgcaccaatg caagcagcaa
     2761 caaatggttt tttcaatggg atcgcaattc cgtaaacagt tggttcggtc actcccgcaa
     2821 gtaaaatcga agcaaaagca gaaccggaga tcgaacgggt ttttgaacgt ttacttcgca
     2881 gaaatacacc taaagctgca ccagccattc caaaattggc tgctcctgtc aacggaacga
     2941 ttcggtcgaa cccataagca gagatattac tgatcatgac agggtttaca gcccattgga
     3001 taccaaatat taccaaaata ttccaaccac cagcgatgat tgcgccactg atcggtccgt
     3061 taatatccat catccaagta attgcggcag ctaaaccttc gccaaggtaa acacccgcag
     3121 gcccgaatac cattaacgtc aaaggaaccg tgataactaa tgaaagtaaa ggtacaaata
     3181 ctaattgcat attttcgcga atatattttt tgaggaattt ttcaacaaaa gaataaaaga
     3241 aaatacctaa aatcgccggc atgacagtag acgtatactg catcagtact acaggaatcc
     3301 ccagaaaatc agtagctgct ttgccactgt ctgaaagtaa agaggtcaga ttcggttcaa
     3361 tcagtgctgc agcaatgaca gccgcaatat atccattcac tttaaacgtt ttggcagcag
     3421 aaaaagcaag aaagattggc agaaaataaa agacggcatt cgacgcagca gacaaaatat
     3481 gataagttcc gcttgtttca ctaagccagc caatcgtagt cgctaaaaca accagacctt
     3541 ttaatatacc agaaccggca aacagcggaa tgatcggcgt aaagatttcc gatatcaaac
     3601 caaatagttt ttcgatcgga tttagctttt ctttcggagc tgaatgatcg atttgacgcg
     3661 tcaattcctt gtaagcagga gccaatccct gatccatgat tatttctgca tatctatcga
     3721 gtaaaatgaa atacttaatg gaagggattt gctgttttaa tttttctgca ttggctactg
     3781 gctgtttttt caatttgata cgaagccgag tcgcacatgg aaggacagaa tcaatattgt
     3841 cttttcctcc caactgttca atcagcatat gaatctgctc ggacagtacc atctgttcag
     3901 gattcattgt cgtttccatt agaagaccac tccactcact aatataatat tggcgtaagg
     3961 tgtcatttcg cctgtacgaa taaatgcatg acagttattc aactcttttt tcatttcttc
     4021 atgtgggata aagctaatcg gtgtatctgg taacaataat ttgatttttt ccaatatttc
     4081 cgggttcatt gtctttattt cttccgccaa ttcaatcgat tgtacctcaa gttcttctaa
     4141 aacgttttct aaaacagata aaaaacttgg aagaccgttt gagacagcca aatctatttt
     4201 ttcagtacca aaggggacag gcatgcctgc atccccgata cttaatttat caaagtgccc
     4261 catttgtgcg attactcgtg aaatatctga attgattact tttccttttt tcatgtttgt
     4321 ttcttcctct cattgttaat tttagactgc tgattgttct aaagcttcgc ggtaagggat
     4381 agaaggttgc gcaccataag actgcacagc aacagaagag gctagatttc cataaaggat
     4441 cgcttctttc aagttactaa ggtctggctg aagtgtgctg cttaatgcgc caataaatgt
     4501 atcacctgcg gctgttgtgt caacggcatc cactttaaaa gcagggacga taccgtgttc
     4561 tttttctgta tgatagaaag cgccggcact gcctagtgta atgattactg ttccaatacc
     4621 taattcatgg agtttttcgg ctgctgcgac caaactgtta tgatctgtca ctctgattcc
     4681 tgtaatgatt tctgtttctg tttcatttgg tataatgata tctgtctttt caagtagttc
     4741 agtaggaatc gtttcacgag ctggtgcagg attcaggatc gttgtttttc ctgcgtcttt
     4801 tgcaatagaa aaagctagga tcgtcgcatc taagctactt tcaaactgag caataataaa
     4861 gtcactgttt gtaatcagtt gcttgttttt taggacttct tgttcatgga aagcattatt
     4921 tgcccctgca tggatcaaga tactattttc tcctgctgcg tctaccataa tcattgcttg
     4981 tccggttgtg gcgccttgga tttcagctat cccagatgta tcgatgtttt cttttgtcag
     5041 tagatctaat aactgttgtc cttcgctgtc agccccaacg cctccaataa aagatgtccg
     5101 tgcgcctgaa cgtttggctg ccactgcttg gttggcacct ttacctccgc cagcgtgaaa
     5161 aatctcatgc gcatgcatcg tttcgcctgg cttgggcatg ttggttaaac gaagggtagt
     5221 gtccatatta atacttccaa ttactgtaac cgtgttcatt ttacaaacct ccaactatct
     5281 catttatttt gatgggaaaa ttatttttta aaacgtttta gtaaaacgtt ttaatagttc
     5341 gataaaaata tagcacacct tgaaagctat ttcaagggtg cagttgaaaa tcttttttct
     5401 aatcgtacag gtaatctttt ttcttcccat tctttttctg ggaattggat ccgatctaac
     5461 aataacttgg cagatgtttg tcccaattcg aagattggtt gtgcgatcgt tgtcagtttc
     5521 ggcttgatat actcgcacat atctatatta tcatagccaa taatgctgta gtcttcaggg
     5581 atgctttttc cagcttcctc taatccgcga tataatccaa aagctaattc atcatttaaa
     5641 gcaaacacac cagttgcagc agaatcaaga agttctgctg tgatctggta gccaccttgt
     5701 tttgagaact gtgtaggtaa gaggatcagt tggtcgtgag ggatttggta aacatcaaga
     5761 gcagatttaa acccctcaat ccgagcgtgg acattttccg gtggattttc aggatagaca
     5821 agggcgatcg tttgatgtcc tagactaagc aagtgcatgc ccgctaggta accaccgcga
     5881 aaatcgtctg ttcgtacggc atcactaaat ccttcagatt ttttctgatc caaaacaatg
     5941 aagggccggc cttgtttttt caaattttcg ttgattgcat ctgtagagac agcagaagtc
     6001 gcgataatga atccatccac acctcgacgt gtgagctcgg ctagatattc gatttctttt
     6061 tgatgatcag aatcagcatt acataatatc gtgacgaaat tctgcttata caagatatcc
     6121 tcaattcctc gcatcagtgt actgaagaat ggattcgtga tatcaggaac gagtacacca
     6181 attgttttcg tttccttggt aatcatttgt ctagcaaaat agtcaggctg atagcctaat
     6241 tcttctttgg cagctaaaac tttctgaaca gttttcggac tgaatttctc cgtatttcca
     6301 tttaatatca aggagacagt tgtgattgaa acgccggaaa gtttagcgac ttctttgatt
     6361 gtgatctttt ttttcaccat gttcatactc ctatctaaat cctgtggtta ttttcccatg
     6421 aaatcaaaaa gaagtaaatg aaaatcacgt gtgccagtac ttatttgctt tgttggatag
     6481 cttcgattat ttgtttcgtt gagcgtactc gtgcagtttt cggaaatatc ttttttatga
     6541 acaattgatg ggattctttg tcacgatcac tgcaagcatc ttcagcaagc acgatatggt
     6601 acccgtgttg aaaggcatct aatgcagtag catatacacc gtttgttgtc gctacaccgc
     6661 ttaaaatgat agtttcgatt ccacgccggc gaagttgaag atcaagagaa gtaccaaaaa
     6721 aagcacttgg attgtatttt gtgatagtca acatattatc tgtatctttc aaagaatcct
     6781 tcaaaagtaa atcagtgaat tctggaggta cagaagcagt cattccaccc atatcagttt
     6841 gttgactcaa ataagggaaa gtggagatgt ctacgttcac taaaactttc aaggcagcgg
     6901 tacgtttcaa tgttcttact aacaaatcat tgtttgtcag aatagatgct gcagtgtgtg
     6961 gagagagtgt accgttattt acgatctctt tttgcatgtc gatcgcgaca aaagcagttt
     7021 ttgataaatc aaaaatcata ttttccttta aatcattcat gatgttcctt ctttcaaata
     7081 acaatgtttt ttatgtaagt aaatcataaa tcgtttattt gtcaactaaa ttaaaggatt
     7141 cctttcttta gtctagagaa attcagtggt aaaataaata agtgtgagaa aacgcattcg
     7201 atgcatagat atagcgagaa atatcgtgaa aatggaggaa tcagagtgaa gtatgtcgaa
     7261 gtaaaaaatg aatacaaacg atacaaaatg ggagaaacga ccattacagc aaatgacgat
     7321 atttcttttt caattgaaaa aggcgaatta gtgattatac ttggtccaag tggagcagga
     7381 aagtcaacga ttcttaatat tttaggtggg atggatacac ctgatgaagg tcagatcatt
     7441 attgatgaca cagatattgc gcaattttca gataaacagc ttactgctta ccgacgaaca
     7501 gatgtagggt tcgttttcca attctacaat ctcgttccta atctaacagc aaaagaaaat
     7561 gtggaattgg caacggaagt ttctcctgat gcgcttgatc ctgtagaggt attgaggcaa
     7621 gtgggcctag ctcatcgatt gaataacttt ccgtcccaat tatcaggagg cgaacagcag
     7681 agggtttcaa ttgctcgtgc actagctaaa aatcccaaac ttcttttatg tgatgaaccg
     7741 acaggcgcgc tggattttga aacgggcaag caagtattga aattattgca aaacgctagc
     7801 agacaacacg gcaataccgt tttgatcatt acacataatt ctgctttagc gcccatcgct
     7861 gatagagtca ttcacattaa tgatgctaaa gtaaggtctg ttgaattaaa tgatcatcct
     7921 tcatccattg atgagattgt atggtagggg gattttatga aaacaagaac atatcttaaa
     7981 gcaggttttc gcgaaattcg gcaatctaaa ggacgattca ttgcaatcat ccttattgtc
     8041 atgctaggaa cacttttatt tgtaggtgtc aaaactgctg gacctgtcat gcaaaaaacg
     8101 atggatcact atgttggtac agcaggactt tctgatttgc aaatcgtttc tactggtggc
     8161 ttgacacaaa aagatattgc tcaagcagaa aagatcccag atgcaaaagt agaaacagga
     8221 aaacagatct attattccaa tactggcaaa aacgaagtga ttcaaatatt ttcttataac
     8281 aaaaataaca agcagaacaa gctacaatta actgatggaa aactcccgga aaaaccaaac
     8341 cagcttgtat tggatgaaaa agcggaagat gaagggtata aaatcggtga tatttatcag
     8401 attgattcag atgaattgaa agaaaaagag tataccatca cgggctttgt ccgttctcca
     8461 ttgttcatca acaatttgga gagaggatac gcgaacgtcg gaaatggtag cgtcgattat
     8521 tttgtctatt tacctgaatc cgtttttaaa acagatattt attctgtctt atatcttgat
     8581 tttacaaacg taaagaattt gaatacgtac agcaaagctt acaaagataa aatggagcaa
     8641 aatcaagaaa aggcagaaaa gtatttacaa gatcgacccg aagaacgctt agaagagctg
     8701 aaaaaatcgg ccaacgaaga attagaaccg gcacagcaaa aaatagcgga tggcaaagca
     8761 caaacaacgc aagcaagaac acggttagaa gatgctaaaa aacagttagc gcaacagtca
     8821 gcaactatcc agcagcttcc tgaagcgcaa cgaacaatcg ctgttcaaac attatctcaa
     8881 caagaagctc aacttgctga acaagaaagg caattagttg agaaagaacg tgaactggcc
     8941 tctgcagagg aagaattgaa tcgaaatgca gaaaaaattg aaaatttaac taaaccatca
     9001 tacctataca atgaacgttc agaaaatgtc ggttttcaag aatttggtga tctgtctgat
     9061 cgtatcgctg cgattgccaa tgtttttcct gttttctttt tctttattgc cgcattgatc
     9121 acctttacga caatgacacg tatggtagaa gaaaaccgca gggaaatcgg gacattaaag
     9181 gcgttaggtt atacgaaagt tgaaatcgca aaaaaatatg caatctatgc aagtctagcc
     9241 tcagggatag gtattgtttt aggaacgata ttaggaacga atcttttgcc aaggatcatt
     9301 tttgagctgt ctaacgagcg gtatgatatt gggagtgcgc ttatttttta tgactggccg
     9361 ccgattttac aagcagctgc cgcattcttc attgccgcat ttggcgctgc aatgatcgtt
     9421 ttattcaaag atttaagaga acggcctgct gcattgcttc aaccgaaagc accgaagcca
     9481 gggaaacgca tttttcttga atacatcact ccgctgtggt cacgccttag ctttaatcaa
     9541 aaaatcagtt accgaaatct cttccgatat aagtcaagaa tgcttatggc gattatcgga
     9601 attgctggat gtgccgggct gatggttgca ggcgtgggat tgaaagattc tctaagttct
     9661 gtttcagcca aacaatttgg accgatcatt gactatcaag caatcgttac attggattca
     9721 tccacggaag atagtcgtca aaaagcagcg gatatgctgc agaagcaaga aaaagtaaat
     9781 gagatcctag ctattcatac tcaaaccgta gaattgagaa aaaaaggaca tgcttcgcaa
     9841 agtctgacag tgatcgtacc aagtaaaaca gctgatttgg atcgttttat ccatttaact
     9901 gatcttgaag agaacccacg ctccttgcct aaaacaggaa tcgctattac gcaaaaagta
     9961 gcagaactat ttgacttatc tacaggagat catatttcgc tttatgatga agatcaaaaa
    10021 gaatggaaag tcgaagtaaa agaaatcgtg aaaaactatc taggtagctt tatttatatg
    10081 tcccctgaat attatgcttc agttactgat caatcgatga tggataatgc atttttgctg
    10141 cgtacagata agatgacaac aaaagaagaa gaaatgcttt ccgagaaact gcttgccaca
    10201 gacgctgtga caaatacgtc tttcatgtcc aaacaaatcg aaacgcaaga agaatcaatg
    10261 gcgaacttag actcggttgt cttaatcttt gtcgtattat caggactgct ggcttttatc
    10321 gtcttgtata atctaactaa tatcaatatt tccgagcgtg tacgcgaatt atctacaata
    10381 aaagtccttg gattttttga taaagaagtc acgatgtaca ttgttcgaga aaatatcatc
    10441 tttactcttt taggaatcat cggaggattt ggtgtaggat atatattgac tgacttcatc
    10501 ttgcaacaag cgtctatgga gaatgtcatc tttccattag tgatcacatg gtgggcttat
    10561 gtcttatcag caggattaac tatcgtattt acggttattg tgatgattgt tacccatttc
    10621 aaattaaagc atatcgatat gattgatgcg ttgaaatcga atgaataaaa acagaggttg
    10681 tgaaatcagc ggtttgacct gagtattaga gaaacaaacg gcaaaatagt ttttcatatt
    10741 ttgccgtttt ttgagctaat accgaaggac agctgattga acaccatgaa tcagaggttg
    10801 taaaaaagcc gtcctgcatc gagcaaaaag aatactcaaa taaaattagc tcctcatgtt
    10861 ttacatttga gaagactgat tgccgtagat aaaagctttt gaacaccatt tatagaagaa
    10921 acaagaatat tttgctattt taaacaagag gatagacaaa tacatgtctg atctcttgtt
    10981 ttttattggg caacagacct cttagaatgt ataaagataa attttacaaa cgctttcggt
    11041 tcagttataa taataaatga taacttgtat atataagttg tggtaggtaa taaatcagtt
    11101 gaatggaggt aaatatccat gacatttaaa gacaaagtca tttaccaaat ctatcccaaa
    11161 tcttacaaag acagtaatgg tgatggaatt ggagatttaa aaggaatcaa agaaaaactc
    11221 ccgtatctga atgatctagg catcgacatg atttggctaa accctattta tccaagccct
    11281 caaaaagaca acggttatga tatctctgat tacacagcca tcgatcctat ctttggtacg
    11341 atggaggaat tcgaacagct aatagccgaa gcaaagaaat accggataga aatcatgctc
    11401 gatatggtgc tgaatcacgt ttcgacagaa catgaatggt tcaaaaaagc actagcaggg
    11461 gaccagcatt atcaggactt cttcatttta agagaagagc caacggattg ggtatcaaaa
    11521 tttggtggaa gtgcttggtc gccatttgga gatacgggga aatattattt gcacttatat
    11581 gatcggacac aagctgactt gaactggcgg aaccaagcag taagagagga actattcaaa
    11641 gttgttcgtt tttggatgga aaaaggggta aaaggattcc gtttcgatgt tatcaatgtt
    11701 atcggtaaag acgaacaatt gaaaaataat aaagaaaatg atggcaaacc tgaatatacg
    11761 gacaaaccaa tcacccatac gtatctaaaa gaattgaacc aagcaacgtt tgggacggat
    11821 tccactatca tcacagtcgg tgaaatgagc tctacgacga tcgaaaattg tatcttgtat
    11881 acagaaccta gtagagaaga attatcaatg gtctttaatt tccatcattt gaaagtcgat
    11941 tatgaaaatg ggaataaatg gtctactcct ccatttgatt ttgcagaatt aaagcgacta
    12001 tttcatacat ggggagaaaa gatgagtgag ggtaacggct ggaatgctct attttttaat
    12061 aatcatgatc agccacgtgc gctcaatcga tttgtagcta tcgatcagta tcgaaaacaa
    12121 ggagcagaga tgctagcaac catgatccat ttgaaccgtg ggacgcccta tatttatatg
    12181 ggagaagaaa tcggcatgat cgacccagac tattcatcga tttccgacta tattgacgta
    12241 gaaagtttga atgcttataa gttactcctt gaacaaggat tcacagaaaa agaagcgttt
    12301 cgtcggatca aagcaaaatc tcgcgataac tctcgaacac ccatgcaatg gtcagacaca
    12361 gaacaagcag gatttactac agggaaacct tggttaaaag ttggaggcaa gctagaagag
    12421 attaatgtag aaaaagaact atcttctgaa gattctattt tgtcctatta caagaaattg
    12481 attcggctga gaaaaacata tccaatcgtt gcacaaggag attatcatgc ttatgaagca
    12541 aatcatccgg aagtttatgg ctttcttcgc caatttgaag gacagcagct gcttgtatta
    12601 acaaatttct atgataaaga tacaaggatc gccatcccag aaggattcac agaagccaat
    12661 attcttatcg ataattattc cgatactgtt cttcatcaaa gtctggagct aaaaccatac
    12721 caggcaattg ctttgctgaa aaattaaaac aaaaacacaa aacgtcgttc ctacgagcta
    12781 ttctcgtaag atcgacgttt tgtgttttat ttaaaataat cttcttcaga aaatgaagag
    12841 ttttcatact ctcctttatc gccataaaat ttattgtagc gttgtttgaa tttacggaaa
    12901 ataaaagtaa cgagtaattt gactaacgta tagataggaa taccaaaaat aacgcccaca
    12961 attcccatta gatcacccat gacaagtaaa acgattaaga tcgtaatcgg atggatctgt
    13021 aaacgatcac ccattactcg aggaatcacc aagtcaccgt ggagaagttg tacggcaaac
    13081 cagacaatga caaatttgac agccataaaa gtagagtctt ggaatgcaat gatcaaacct
    13141 ggaaagaagg cgataaacgg cccaatatat ggaatgatac ataacaaccc agcagaaata
    13201 gctaaaacac tagcaaattc taatccgatc agtaagaaac aaacccagta gactgctcca
    13261 aggataaatg aagcaatgat ttgccccttt agaaatgaac ccaattgttg attagcaatc
    13321 tttgtcaaat ttttaaaatc tgtacgaaat gctggtggga caatcgctaa aactgactga
    13381 taaaattttt gaggatctct caaaaggaaa aaagcgacaa ttggaccagt aaataaagta
    13441 ataaaaatag ctgtaacagt tgaaaaaatg ttgctgatcc gttgtgcgcc actttcccaa
    13501 taatttccaa taaatccagc tatatcagac gtcacgtcgt caatagaagc gaaaaattga
    13561 ttaaatgaat cggcaaaagg tgttttctcc aaaaattgtc ggagactctc ttgaaaatct
    13621 tgaaacaaat ctggcaaact actgatcaat gtctttgcct gttcttcaag aaaaggaata
    13681 agccatatgc ttccaagccc caataaaaat aaaataatca cataaacgat aatgactgac
    13741 aagttcctcg aaaggtgctt gctcaaacgt ttgatgactg gttccaaaag ataatacaag
    13801 atcagtgcaa acaataatgg gggcaagata gtagcgataa tgattccgac aggcttgaat
    13861 ataaacgaga tctgatggaa aaaataaatg atcagcgcaa gtaagataac tattcctaaa
    13921 acatagtaag aggtttttcc tcctaaaaag cggatcaaac gattcgttgt atctgtttct
    13981 tttttcactc tttttcctcc tttatctata acttcattat agacaaaagc gggtataaaa
    14041 ggaaaatgat agaggttgat tgcaaaaata tcgtcaatca ttcatataaa ctttctcatc
    14101 aattaaaata agaaaacgct tgacagttct tttaaccata tgataaatta aatcctgttg
    14161 ctaaaacgta actattacgg tttgcgaaaa gcgaatcgga acattcaaat gaaagaagat
    14221 gagaagagga gtgtcataaa tgaaacgatt gataggtata ttggccatgc tggttttagc
    14281 aggtatgtta actgcttgcg gggcaagtgg gaaagccgag gacagtaaag aaaaattgag
    14341 cgtgatgacg accttttatc ccatgtatga ttttacaaaa gcaatcgtcg gggatgaagg
    14401 agaagtagag ttattgattc cagcagggac tgattcccac gattatgaac cttctgctaa
    14461 agacatggca aagatccaag atacagatat ctttgtttat aatgatgaaa acatggaaac
    14521 atgggtcccc gcaatccaaa aaacattaca agaaggcaat gtccatacga taaaagcgac
    14581 agaaggtatg cttttacttc cgggaagtga agaaggacat gatcatgacc atgaacacgg
    14641 ggaagaaggc catacacatg agctagaccc acatgtatgg ctagctccaa gtttagcaat
    14701 caagcaggta gcaaacatac gtgatcagct gattgaagct tacccagaaa aacaggaagt
    14761 atggacaaaa aatgcagcag cctataccga gaaacttcaa gcactccatc agctttatca
    14821 agaaaccttt aagcaagcaa aacaaagatc ttttgtcacg caacatgctg cattcaacta
    14881 tctagcgtta gaatatggat tgaatcaagt atcgattgct ggtctttctt ctagtgaaga
    14941 accgtctgcg gcgagaattg ctgaactgaa acactttgtt aaagaacatg gaatcaacta
    15001 tatttatttt gaagaaaacg ccaaagatag tattgccaga acgttagcaa acgaagcagg
    15061 tgtatcttta gaagtattga atcctttgga aggattgacg aatgaacaaa tagaaaatgg
    15121 agaaaactat ctttctatca tggaagcaaa cctcgaagcg ttgaaaaaaa cgacagagac
    15181 agaaaacccg ctagaagata ctttgacccc caaaaaagaa aagagcgtct acaacggtta
    15241 ttttgaagac gaggatgtac aagatcgctc tctttcagat tgggcaggaa cttggcaaac
    15301 agtagatacg tatgtagaag atgggacttt tgatcctgtt tttgaatata aagaaaaatt
    15361 gaatcaggat aagacagcac aagaatataa agaatattat acgaagggct atcaaacaga
    15421 tattgcttca atcaagatca gtgagaaaga gatggcattt acgtttaaga atggaacaac
    15481 gaaacgttca aattatcgtt atgtagggaa agaaatcttg acctacgagg caggaaaccg
    15541 tggcgtaagg tatctattcg aagcagaaga taaggaaagt ggcgctttta gatatattca
    15601 attcagtgat cacacgattg caccggctga ctccgaacat tttcatttat atgcaggaaa
    15661 tgaaagtcaa gaagtattgc tgaaagaaat ggagaattgg ccaacatttt atccagatac
    15721 aatggatgga cgtgaaattg cacaggaaat gcttttgcac tgataggcaa aaaagaaccg
    15781 aagggtttgg atcggctggt catttagtcg gtccaaacta ttcggttctt tttatgaaat
    15841 caatttcttt aatccacgga tagaggctgg gatggtctcg ccgtttgcca acgtgacttc
    15901 tctggttttt ttattaatcg accgcacatt ttgtgtattg ataacatagg atttatgaca
    15961 acggataaaa ttccgatgca ttttctcgat ttctttgatc ttctcataga attgtaattg
    16021 ggcatgagtc aaatggactt ccagtttatg tggcgctcct agcgaagtag aaaaatattg
    16081 gatgtctttg atcggtaaaa atttgatctc atcattaaac tctattttga agatatcttt
    16141 ttggatttgg gacatttgaa aatcttcgaa aattttgctc aggatatgct cgacattttc
    16201 ttttaaatca taaagatctt ctttgacgat ataatcagtc ggttcgatgt tgctttttaa
    16261 gattttcatc gacatttccg tatgggaagt cacatagatt atttttccta aaggatctaa
    16321 ttcacggatc aatttaccga tcgctacacc atccatttca ctgtcttcta gttcgatatc
    16381 taaaaagtaa ataccaaaag aagaagttct gatcttggct gcattgagaa gttcttccgg
    16441 atcagaggta gaaatatgga cataactgtc taagttttca atcatgattc tatttttgat
    16501 caccgttgat aaaacttcta attgttttgg atcgtcttca caaatataaa tgggaatcat
    16561 atcattactc ctttatttta tttgtatata aatcgtttga gaaaaacgct ggttttcaat
    16621 tgatgtttca aggaaaagat aaggattggt ctttgtcaat tcatccaaga taaacaagcc
    16681 aaggccttcg ttttttggat ttgtttttgt tgagaaccct ttttgtttca actggtttaa
    16741 cggtaatgta tttgttgttg tattgatcac acgaatcatc acggaatgat tcttcttgag
    16801 tatggtcaat tcgatttttt tcttctttgc agcactagct ccttcaatcg cattgtccaa
    16861 gataatacca agcatacgta caaggtttac cgtatgtttc gtatccagtt cgattggctc
    16921 aggaaaatga agctggacga tcaacccttt ttcctgtgcc atcatgagtt ttaatgacag
    16981 taaactacgg atctcaggaa ctttcatatt atccaacata cttagattgg ccggaaaaag
    17041 gttcatcatc tggcgtgtag gtaatatcgt ttgatcataa taagaccgta atccttctaa
    17101 gtcgccttct gaaatatagc tgtgaagaga aaaaagcaga ttttggtaat catgtttgaa
    17161 ttctctcaag tcttgatagt ttttttctag atctttgata taatcctgtt gcatcctgac
    17221 ttgttgctct aataactgat cagctaattt cttattagag attttccaat aataaaccaa
    17281 gatgatcagg ccaacacaca gatatagaat gaaaaaactc gtattcaaag ctaagacatc
    17341 aggtgtttca ccagaaaaac gggtataaaa aataatgata tagtatgtaa ataatatcaa
    17401 tgtcccgatc gtaccgatca tacggcggtc cacagtgtga aaccaagtct ctaacgattt
    17461 ttttaaacaa aaagctaata aagcagataa aaaagcggat aagacaatgt gaaaatactg
    17521 caatgaatct cctggttcaa taaccgaaga tttcaaaaca aaaaaatcgc cgatgcttgt
    17581 taagtgatct cctaaaagcg acgtcaataa cccaaaagga agcgcgtaaa ccaattgttg
    17641 acgcgaagtc gaaaagaaga aaaaagtaag catcagcatt cctatagcaa ttagagcagt
    17701 aaaaatccct aaaatcgaat agacccatcc aatcaaaata caagaaatca gaatgactcc
    17761 cgcaaacaga tatttgtatc ggatgtgatg gtcaatcaat aagatacaaa ttgtgtaaat
    17821 caaatattgc aacgctatgg accataaaaa cataaagtac agctcccatt gatttttacc
    17881 aaattatagc acaattggcg tattattttt ttaatttttc agggattttg aattcagaac
    17941 tcataccaaa gcagcagcca gatacaaaaa agtcagataa gttagttaat tgtttaatga
    18001 ttgttttttt catgaaagat tcctccaaaa ttcatcaagt agtttgtaat aggaagcaac
    18061 gtagctattt ctaaaacaat cccaagtagc aaataggtag aaaagtaaga agctgagaaa
    18121 aataacgtaa tgagagaaaa aattagaaac atgctgatcg ccttctttaa taatggggga
    18181 cgtttctcgg gttcaatggg attgattgct gtacctcttg atccatagcg aagcagtaac
    18241 aaaaagcagc tgattaacaa aaagaccatc attgggaagg agatagtcaa ttgcagatgt
    18301 ttcatcacca agggaacgcc ccataaataa agtagtgtcc acagcagaca aacgaattca
    18361 gagtcggaat gcaatccata agcatatctg cggatgcaga tataagaaag aaaggtgatt
    18421 cctgtttccg cccatgagcc tgtcaccaga gaaaagacta gaacgaccgc tagttttcca
    18481 agattattca ataaaacttc taagacaaat tttacttgga catacaccat atcttcttcc
    18541 gatttgtctc cgctgaataa ggcttctaat aatttgttca caactttatc tttcatctca
    18601 tacgtacctc caccacaaat tttatgctaa aaaaaataaa aaacgtcttt tgtttcataa
    18661 aaagtggttt tgacatcaaa accgtcatga tctcctaaaa aaatcttcgt tttccaagaa
    18721 ccttaccaaa aaaagtgaca gttttgatac gaaaagtact ttttatgaca agtttgtgct
    18781 ttttcgagtg atttatgtta aaaaattact gaccttataa aaggtgatgg agggattctt
    18841 atggaaaatg aacaattgtt tgaagtcggc gaaacatatg catttaaatc tgatttattt
    18901 gaatcaccaa taaaaggaaa aatagaaaaa atctatgaaa atagtgtgat gatacgtgtc
    18961 atgaaatgcg cag
//
LOCUS       DANKKG010000047        18616 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000047 DANKKG010000000
VERSION     DANKKG010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.047"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(9..530)
                     /locus_tag="PW004_001364"
     CDS             complement(9..530)
                     /locus_tag="PW004_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HDL2726346.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            729..4421
                     /gene="nifJ"
                     /locus_tag="PW004_001365"
     CDS             729..4421
                     /gene="nifJ"
                     /locus_tag="PW004_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HDL2726347.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            4610..5497
                     /locus_tag="PW004_001366"
     CDS             4610..5497
                     /locus_tag="PW004_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HDL2726348.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            5494..6663
                     /locus_tag="PW004_001367"
     CDS             5494..6663
                     /locus_tag="PW004_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726349.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            6687..8042
                     /locus_tag="PW004_001368"
     CDS             6687..8042
                     /locus_tag="PW004_001368"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HDL2726350.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(8114..9919)
                     /gene="glmS"
                     /locus_tag="PW004_001369"
     CDS             complement(8114..9919)
                     /gene="glmS"
                     /locus_tag="PW004_001369"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HDL2726351.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            10328..10618
                     /locus_tag="PW004_001370"
     CDS             10328..10618
                     /locus_tag="PW004_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726352.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            10631..11656
                     /gene="sppA"
                     /locus_tag="PW004_001371"
     CDS             10631..11656
                     /gene="sppA"
                     /locus_tag="PW004_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HDL2726353.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            11659..12318
                     /locus_tag="PW004_001372"
     CDS             11659..12318
                     /locus_tag="PW004_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HDL2726354.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            12475..13407
                     /locus_tag="PW004_001373"
     CDS             12475..13407
                     /locus_tag="PW004_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726355.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            13561..14850
                     /locus_tag="PW004_001374"
     CDS             13561..14850
                     /locus_tag="PW004_001374"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HDL2726356.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            15097..16347
                     /gene="clpX"
                     /locus_tag="PW004_001375"
     CDS             15097..16347
                     /gene="clpX"
                     /locus_tag="PW004_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HDL2726357.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            16430..17017
                     /locus_tag="PW004_001376"
     CDS             16430..17017
                     /locus_tag="PW004_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HDL2726358.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(17091..17246)
                     /locus_tag="PW004_001377"
     CDS             complement(17091..17246)
                     /locus_tag="PW004_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726359.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            17471..>18616
                     /locus_tag="PW004_001378"
     CDS             17471..>18616
                     /locus_tag="PW004_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726360.1"
                     /translation="MTRLLIQPTFIRSFTQQADPYDLRKLQEKIMLASVRDESWLVVG
                     NDENECSFRLEDNQLLIKNVLSISVFKEKQFLIRDFIQKKMIAHGVFAYMRAYSEFIY
                     HNTKQISQRLLFEKKDEIEHLPKMKQQNGEVVVDCNQFPGYDLFYQGLCFTSCWEMYY
                     SRYYHQIIPKPIFLDIQQVEKVKELENEVICIQLYRDPFNWQKPNNQMFQSYFRDQLG
                     FDHLAWDNGVGLLKPPFVEYIYTDHTIQSVQYQNAQMQPVPKKNASFFVTKSYDIQQG
                     DYKERRVRGTLNAQAYFPWVDENRSRMMCYKVIDPTVALDNGIEAYCYYIREYLEVEV
                     TDEKYQEYLLSLRIYVPSESLSELPLKEIKHRLSDVTFKRIKKKRRSI"
BASE COUNT         6093 a         3320 c         4019 g         5184 t
ORIGIN      
        1 ttattttttt attttgtttg gatcaattct tttaattcct ctattgaaat agattcatca
       61 aagatttctt tacccaagat gacagttgga acaaatcgaa tatgtgcttg ctcagcttcg
      121 ttgatgacag cttggctcag ctgttcgtct ttttgttctg tcagctttag tttatctact
      181 gcaaaatccg ctacttcttg gagagaaaga tgtttccact cgtcttgagt atcgaatatt
      241 tgtttgatct ctttgatcgc tttttggcca tcgctgattg tgagataccg gtgcatcacg
      301 ttccctcgct gtaagctttc tttttcttta tcgaacaatt tgatcactcg ctgcaattgc
      361 ccgctttgga ctgcttcttc taatagctca taagattctt caaaccattg tcggcaatac
      421 ggacaagcta agttgatgaa ttcgaccatt tttttaggag cattcgatga accataaagg
      481 attccttttt gtgtatttgt tttcgttgca tcaatcactg atatatccat gttaaaacac
      541 ctctttcgct tatagtttat acatgtctga caaaaaaagg caacgattta gactgtttgt
      601 ctaaaaatta gatgaccaat tcaaatagat acatgtaaaa tatttttata aataaaagaa
      661 aaaaatggca tgttttacgt tataatggaa cagataataa gagagaatat gaacaaggat
      721 gatgaatcat gcgaaaaatg aaaacaatgg atggcaatac ggcagccgca tatatttctt
      781 atgcatttac cgagattgct gcaatatatc caataacacc cagttcgacc atggctgaat
      841 tagtagatca atggtcagca gcaggaaaag aaaacgtatt cggtcaacca gtaaaagtag
      901 tagaaatgca atccgaagca ggagcagctg gtgtggtcca tggatcattg aaaactggtg
      961 cactgacaac tacatatacc gcttcacaag ggctgctttt gatgataccg aatatgtaca
     1021 agattgctgg ggaactactg ccaagtgttt tccacgttgc tagtcgggca gtgacgacaa
     1081 atgcgttgaa tattttcgga gatcacggag acgtgatggc tgcaagacaa acaggatttg
     1141 ctatgttagc tgaaagcagt gtacaagaag tgatggattt agcacctgtt gcccatcttg
     1201 cttctattga aggagaagtc ccttttttga atttctttga tggattccgt acgagtcacg
     1261 agattcaaaa aatcgaagtt ttggaatatg aagaattagc tcctctgatc aatcaggaga
     1321 aattagcttc atttcgtaaa cgagggatga atcccaatca tccgactgtt agtggtacga
     1381 accagaatcc agacatccat tttcaacaac gggaaacgat caacccctat tatgataaat
     1441 tacctgaaat cgttcaaaaa tatatgaaag agatcaaccg attacgagga acttcttatg
     1501 acttggtgac ttattacgga gcagacgatg cggaagaagt cattgtttct atgggatcag
     1561 ttgcacagac gattgaacaa acgattgatt acttgaatgc aaacggacgg aaaacaggtt
     1621 ttttgaatat ccatctttat cgtccttttc cagtggaaac attcgtgcaa aagttgcctc
     1681 atacagtaaa aacaattgct gttcttgatc gaagtaaaga accaggggca ggtggagaac
     1741 ctctcttatt agacgttcaa agtgcgttat atgattctga acttcgcccg gctgttattg
     1801 gtggtcgcta cggattgggg tctaaagatg tgactcccga ccaaatctca gcagtttttg
     1861 atgaattgaa aaaagaccca agcatacgca aaaaacggtt taccataggg atcgtggatg
     1921 atgtgactta tcaatctttg gaaccaaagg aaagtctgga tttgacagaa ccgcaaacat
     1981 tccaggccaa gttttgggga tttggatctg acggaacagt tggagcaaat aaatcagcta
     2041 tcaagattat tggagatcac accgataaat atgcgcaagg ctatttttat tatgattcga
     2101 aaaaatctgg cggtctgact gtctctcatc ttcgttttgg agatacgccg atacgttcag
     2161 cgtatcttgt cgagcatgcg gaccttgttg cctgtcacac accagcttac ctgcatagct
     2221 atgacttggt caaaggattg aagccaggtg gcattttttt attgaatact ctttggagcg
     2281 acgaacaatt agaaacgcat ctgcccttaa aattaaaacg ttacttggct gaaaataaca
     2341 ttagattcta tacgatcaat gccatgagat tggcccaaga agtcggtttg ggacgccgga
     2401 tcaatacggc aatggaaacg gcttttttta aattagctga catcattcct tttgatgagg
     2461 tgcttccgct gctaaaagaa gaagccctga aaagctatgg acataaatca atggctatcg
     2521 tagaaaaaaa catacaagca atcgatcgga cgaccgaact gcttcatcaa gtcgcgatcc
     2581 cagaccattg gagaacgttg gagatgcctg aaaaaatcaa taaaaggaac actggttata
     2641 tacaagaaat acttgaacca gtgaatgcac aggaaggaaa tcaactctct gttggtacac
     2701 ttgtagcaaa tggaatgaca actggcgaaa tgcctttagg aacggcttct atggaaaagc
     2761 ggggcgtcgc tttagaagta cctgagtgga ttagtgatcg ctgtacgatg tgtaatgaat
     2821 gtgcctttgt ttgtcctcat gctgccatcc gtccattttt agcagatgaa gaggaaatgg
     2881 aagaagcgcc agaaggattt atcgtgagag aaatgcgagg cgcagacggc gtaaaatacc
     2941 gtatccaagt ttcagtggaa gactgtacag gttgtggtct gtgtgtagag gcttgtcccg
     3001 caaaagaaaa agctttagtg atgaagcctt atgaagaaca gaaagaacaa gctgtgaatt
     3061 gggcttttgc aatgacatta agacaaaaag caaatccagt caaaggaaag aatactgtct
     3121 tgtctacaca attcaatcaa ccgcttcttg aattctcggg cgcttgctca ggctgtggcg
     3181 aaacacctta tgtaaaatta ttgacacaaa tgttcggtga tcgaatgatg atcgcgaatg
     3241 cgacaggctg ttcttctatt tggggcggtg cttctcctgc ttctccttat acgaccaatg
     3301 aatgtggtca agggcctgca tggtcaaatt ctttattgga agataatgct gaatttggtt
     3361 atggtatgct tctagcggca cagactcgtc gcgaggcttt ggcaagttca atcaaagaag
     3421 tattgccaaa agtatcagaa gaactacgag aattgatgga agactggtta gtacacatgt
     3481 ttgagagtga aggaacgcag caaagagcag caaaactgca agctgcgatc gaaagcgaac
     3541 gatcaatttt ccctgaactg gaggatatat acaagagaaa ggatctattc gtaaaaaaca
     3601 gccaatggat gattggtgga gacggatggg cttacgacat tggttttggc gggatcgatc
     3661 atgtacttgc cagcggagca gacgtgaata tccttgtttt ggataacgaa gtctattcca
     3721 atactggcgg tcaaacctca aaagcaacac cagcttcggc aatcgctaaa ttttcggcta
     3781 gtggaaagta tgcttcaaag aaagatctag gaatgatggc gatgacatat ggaaatgttt
     3841 atgtggcgca aattgcttca ggtgccaatc aaatgcaaac gatcaaggca tttgaagaag
     3901 cagaaaagta tcctggtcct tctctgatta ttgcctatac gccttgtatc acacatggct
     3961 tgcttggcgg aatgaaagaa tcgcttaaag aagcaaaaga agctgtttct tctggttatt
     4021 ggtcattata tcgatataac cctacaatgg tcgaaaaaaa caagaaccca atgactttgg
     4081 actacaaaaa acctgatttc tctgctatgc ctgatttcat gaggaaacaa gttcgctttt
     4141 cctcgctaga taacgcaaat ccaaatcttg caaaagcttt atttgaaaag actgtccacg
     4201 acgctaaaac acgtttttac aactatgctg cactgacggg agaattagaa aaaatcaaga
     4261 aacgacttga accagaggaa tctatcgatg tacccgttaa gactagaaaa gaaaaaacga
     4321 cggaccttga agcagaagaa agaagagctg ctagaagagc tgccagagca gaacgacgta
     4381 aaaaaatgga acaagctaca aaagaaaaaa aggaaaaata acagaaaaaa tataatgaat
     4441 aattctctga agtttaaaaa tatttattgc agagaaaaat aaaacgcata cttgcaattt
     4501 tcttgaaaat tatgcgtttt tctcaagaaa aatacgagca tatcagcctc taaaattaaa
     4561 acatatgata tactagagag gaaatctcaa aagagaagag aggcttttta tgtcatttca
     4621 aatcggggaa ctttttaatt tggactattg gcagcaaatg ttttccggtg acttgttctc
     4681 tctgaatttt ttggtgaaca tacttgatat tctggttgtc tggtatcttg tatacaaact
     4741 gatccaactg ctgcgaggca caaaagcgat tcagcttctg aaaggtgtag ctatttttat
     4801 tgttatccga atcgtagcgg aaatcattgg tctgcacacc ttgtcttggc tgatgaacca
     4861 agtcattacg tacggggtga tagctgctat cgtgattttt cagccggaaa tccggagagg
     4921 actggaacat cttggacgca gttcgctttt caaacaatca aaaagcgaac agcaggaaga
     4981 tgagaagatg atcttgtctt ttgacaaagc catccaatat atgtccaagc gaaaaatcgg
     5041 tgcgttgatc actatcgaac gtcatactgg attagatgag tatattgaga cagggattgc
     5101 tttagatgca gatatcactg gggagctgct gatcaatatc tttattccta atacaccgct
     5161 gcatgacggt gcagttattg taaaagaagg aaagatcgca gtagcaagcg cctatcttcc
     5221 tctatcagaa agtatgttga tccctaaaga attcgggact cgacaccggg cagctgtcgg
     5281 tataagcgaa gtcagtgacg cgattaccat cgttgtctct gaagaaaccg gggacgtcag
     5341 catcacgtta gataatgaac tgatggctgg cttatcccag caagaatatt tggcaatctt
     5401 gcgtagagaa ctaattgctg aagagaaaac aaaaaacaag aaaaacgtga ttcaatcttt
     5461 cctagacggt atgactaagg gaggggatcg caaatgatct caaaagagaa acggacaagc
     5521 attctttaca gttttattgc attgctgttc agtattgcgc tgtacttcaa tgcaaatggc
     5581 cagtccgttc caaatacgct ttctggaaat gaagcgtata atcaaacagt tacgggggtg
     5641 ccgatcaaga tttcttatga ttcaaagcgc tactatatcc atggatatga aaatgcagtg
     5701 actgtcaaac tttccagtgc aaaccgagtt cagttgaatg cagaggcaaa cgaagataca
     5761 cggatgttcc gcgtcacagc agatatcact aagctgggcg aaggaacaca tgaagttccg
     5821 ctaaaaattc aaaatttgag cagtgctgtc acagcaaaga tcgaaccatc gactattaca
     5881 gtaacggttg agaaaaaggt aataaaagat ttcgatgtgg aaacgttgat tccttcaacg
     5941 ttcacaccat ctggatatga acttgatgat acttccgtta gtcctaaaaa agtctctatc
     6001 actacaggtg acaaaacatt ggcagaaatc aaacgagtag tagctgatgt ggacgcttcg
     6061 atggtcacag atgatgggat taatagtgag gtcccaatac aggcgctgaa tgcagcaggg
     6121 gaagtattaa gtatcgtctc tgatccagtg caagtaacag tgagagctga ttcgatcaag
     6181 ccaagcaagt cggtacgact ttatgggata cagcaaggaa caccagctgc cggggtagaa
     6241 agctatgatt tcagtttttc aaaactggaa gcagaagtaa gcggctctag cgatttgctt
     6301 gcatcgatcg gtgacagtat tgctgtaccg atcaatgtct caggtatcaa tcatcgaaca
     6361 actcgtaaaa ttgaaatacc agtagaagag gggttaagta tcaagcccga atcagttagt
     6421 gtggagatta cacccgtgtt gcaaacgtca agcagtagct ctgcttcgac gagcagtcgg
     6481 acacaagaaa atacaagtac gagtagaagt atttcatctg ctgattcagg aacggaacca
     6541 tcgacaacaa ccatttcttc cagttcggaa tctgggacaa gcagctcgac cacaagtaca
     6601 gaagaaacat cagaaacaag cgaatcaaca caaatgagca gaacaaattc ctcagaaaat
     6661 taagttaaag gagtaatttt acaactatgg gtaaatattt tggaacagac ggcgtacgcg
     6721 gtgaagccaa caaggaactt acgccagaac ttgcatttaa gctgggacgg tgcggaggtt
     6781 atgtcctaag ccagcatgaa gaagaaggaa aacgcccacg cgtgttagta gggcgggata
     6841 caagaatctc aggtcaaatg ttagaagaag cgctgatttc aggactttta tcggtgggta
     6901 tcgaagtgtt ccagctaggt gtgatctcta caccaggtgt tgcttatctg acacgtttgc
     6961 aaaaagcaag cgcaggggtc atgatttctg cttctcataa tccagcacaa gataatggaa
     7021 tcaaattttt cggtgcagat ggttttaaac tagtagatga ccaagagcta gaaattgaag
     7081 cattattaga tgctgaagtg gatgaattgc ctcgtccatc agctgaagga ttgggaactg
     7141 tagaagaatt tccagaaggc ttattgaaat attctcaatt ccttgtacaa acgatcaacg
     7201 gtgacctttc cggtttgacc atttgtgtcg atgcagcaaa cggagcaact tcaacaagtg
     7261 ttaatcgttt gtttgccgat cttgaaacag atttttatac aatgggcaca aatccagacg
     7321 ggttaaatat caatgacggc gtaggctcta cccatccaga acgtttagct gagatggtcg
     7381 ttgaaaaagg agcagatgct ggactagctt tcgatggcga tggcgatcgt attattgcgg
     7441 tggatgaact tggaaacatt gtcgacggtg ataaaatcat gtttatctgt gctaaatact
     7501 tggcagaaaa aaatcgattg aaaaaagata cgatcgttac gactgtcatg agtaacttag
     7561 gattccacaa agcggtggaa ggaatcggtt tgaaagatgt cgttacacaa gttggtgatc
     7621 gttacgttgt agaagaaatg cgcaaaaacg attataactt tggcggcgag cagtctgggc
     7681 acatgatttt cttagatttt aacacaactg gagatgggat gctttcagga attcagttac
     7741 tgaatatcat gaaacagaca ggtaagaaat tatcagagct tgcagcagag gtaacaatct
     7801 atccgcaaaa attagtgaat atccgcgtaa gtgataaaaa cggtgcgatg gatgttccgg
     7861 caatcaaaaa agtgattgaa gaggcagaag ctgaaatggg atcagaagga agaatcctag
     7921 ttcgtccttc cggtacagaa ccattacttc gagtgatggc agaagcgcct actgatgaga
     7981 aagttaatta ctatgtcgat aaaatcgcgg cagtagtcaa agaagaaatc ggactggatt
     8041 aagagatatt ttcataaaaa ataaaagaac aaggaccagt gtcagatgtc tgacactggt
     8101 ccttgttctt ttattattct actgtcactg atttcgctaa attacgtggt ttgtcaacgt
     8161 cgaatccccg taatagcgtt gcgtaatatg caagtaattg agtcggtacg actgtggtta
     8221 gagtagttag ataagggtgg acagttggta gcaccagctg gtcactttcc ttttctaatc
     8281 cctctgagac aataactagt gttttagcgc cacggctttc gacttctttc aggtttcctc
     8341 gagtatgcgc accagtcact tcttcactga tgattccgat cactggtgtt ccttcttcga
     8401 tcaaagcaat cgttccatgt ttcaattcgc cagcggcaaa accttctgcc tggatataac
     8461 tgatttcttt caatttcaaa gcagcttcaa gagaaacgtt gtagtcagca ccacgtccaa
     8521 tgtaaaaggc atttcttgta tcgctgagat atacttctgc caactcttct aaataatctt
     8581 tttgggcgat gaccgattcc atcgcattag caactaaact caattcatga gtgatatcga
     8641 atacaagaga agtttcaaat tcttttttat caccgatagc tttggctaat aaagttagta
     8701 cagctatttg tgcagtataa gctttcgttg atgctactgc gatttcgggt ccagcatgca
     8761 atagcagtgt aaaggatgct tcccgcgaaa gagtagaacc agcaacattt gtaatcgtaa
     8821 gtgaagggta gccgagacgg ttgattttta ccagcacttg gcggctgtct gctgtttcgc
     8881 cactttgact caagaaaata aagaatggtt tttttgaaag aagaggtgtg ttatagccaa
     8941 attcactggc taaatgaacc tcaacaggaa tctgtgtcaa tttttccaaa gtctgtttac
     9001 ctgctaaccc cgcatggtaa ctagttccgc aagcaacgat atagattcga tcgctatcag
     9061 acaatgtatc gaccagcttt tgatcaagag agacacgatt gtcttctgtt acgtattttt
     9121 gagcgatacg acgcatgata ataggctgtt cgtcgatttc tttcagcata taaaaaggat
     9181 aggtaccttt ctcgatatct gaagcatcta cttgtgcttc gaatgaagaa cgttctacga
     9241 tgtcacctgc aaaattttga ataataactt tttctgatgt gacggtaacc atctcttcat
     9301 cttcaatctc aacgaattcc ttcgtatgag caagcatagc cattgcatcg ctggcaatca
     9361 cattgaatcc atcgccgagt ccgattaata gtggactttt atttttagcg acgtaaataa
     9421 catcaggtgc tgtccgatca atcaaagcga aagcgtaaga acctttgatg acccgcaaag
     9481 cttttaaaaa cgcctcttgc gttgtagtat ccttcgcgaa agtttcaatt agatgagcga
     9541 taatctcagt atcggtctca ccaataaagt ggtcatttgc taaataagca attttaagtt
     9601 cttcaaagtt ttcgattacg ccgttgtgaa cgagcacgaa gcgtccactt tgggaagtat
     9661 gtggatgggc gttttcttca gaaggttgcc cgtgagttgc ccatcgtgta tgaccaatac
     9721 ctatatttcc tgtagtatca atatttattt tgtttttcaa attttgaatt ctgccttgtg
     9781 ctttgaccag ataatcagtt gtgccatctg agacaaagat accagctgaa tcataaccac
     9841 ggtattctaa tttttctaga ccgtcaatca atcctggtgt tacatttttt aatccaatca
     9901 ttccaacaat tccgcacata aaaaacatcc tactttctat aatgaattcg tatgaatgct
     9961 tccagctgca ggaaaatcct tttgttttat ctttgcagac attttttgtg gattttctgt
    10021 agagtgcagt ccctagggcc atccgccgaa tctttcgata aaccctctcc tcgtcatctg
    10081 tttacacaga cctggcgcta tcctaaaagg tataactatc ctcctttttg ttttttatct
    10141 ggaaacattt aaaatcataa ccttttgcga atgttcttgt caacaaaaaa agaataaaaa
    10201 ataaattggt atatatcaaa ttctttgtca aatgtccttt ttatgtcaaa aaataatggg
    10261 tttttatcta tcatcttttt caggagttcg gtacaataga aagtaatcgg atagaggagg
    10321 agtaaaaatg gattcaaaaa ttgagatcat gacactggga atgttaaaaa aacaattgtc
    10381 caaattcgaa gcatcagctg gtgtatcaga tgatacgaaa atatttttag atactggctg
    10441 ggatagcatc caagaaatcg cacctgatgc cttggaagtt gctcaagctc gtgagtttac
    10501 ggtagaagat gaattaacaa aagagtcgtt ttccgggtat gcgcgggaag aaaaagcaga
    10561 gcggttcgac acttctgagc agtcagaaac tgtgatcgtc ataaaaaact tgtattagaa
    10621 aggagaaaga atgaataaaa gacgttgggc agcagttctg attgctgcag gattatttgt
    10681 ggtttcgtta gtttcagcta gtttgactaa gcctcaggaa aacgatcaaa ttgagggatt
    10741 gaacagttgg ttatatgggg atgaagaact agctccaaat gtgttggaag agggagacag
    10801 tacgcaaaaa atcgtaaaat tatcagtaga tggaacgatt gctgcgggag ggtcttcagg
    10861 tttatttgct tctgaaggtt acaatcataa aaattttatg gaacaactga aggcgattga
    10921 agaagatcca acagtcaaag gtatcttttt agaagtaaat tctcctggtg gcggtgtcta
    10981 cgaaagtgcg gaaatcgcta agaaattgga tacgattcgt aaagaacatg atatcccaat
    11041 gtatgtcagc atgaaaaaca tggcagctag cggtggctac tatatttctg ctcaagcaga
    11101 taaaatcttt gcaacagaag aaacagtgac cggctcaatt ggtgtgatca tgtcaggatt
    11161 gaattattca ggacttctag aaaaactggg tgtggaagat acaacagtta aaagcggagc
    11221 gttgaaagat atgggttcag gaacgcgacc agagacaaaa gaagaagaag cggtgcttca
    11281 agcatatatc gacagcgcat atagtcgttt tgtagcaatc gtcagcgagg gacggaataa
    11341 atctgaagaa gaagtcaaaa aaattgctga tggaaggatt tatgatggtg tccaagcaaa
    11401 agaagtaggt ttagtcgatg aattgggatt tcctgaagat gctttaaaag ctatacgaaa
    11461 ggaacaacag cttgaagatg cggaactagt agaatattct tccacgccat ctgggtttgc
    11521 tagtacatgg ttaggaagta aactagcaga attccaggga ttgaagcctt ctgagactag
    11581 tcagatctta tccgttcttg agaatctagg gacagcggaa tcaccaaaag caatgtatta
    11641 ttacggaggt gagtaagcat gtcagagctt tcaaatgaaa ataaggaaca agaaaaagat
    11701 aagcaacagc agcctgctgt aggacctgac tccaactttt cccaaaaggt actgagctct
    11761 tttgaaagag agcctttgac gcccgaagaa atcaaaaaga aacaacgtaa atggaaaaag
    11821 tatacggaca caagaaatga accgccaaaa gcaaatgatt ttcctaagta tttctatgca
    11881 ggttttttta tccgaatgtt tgccttcttg gtcgatcttt tgtgtatcgg agctattacg
    11941 cgtttaacac ttggtattgc caaaaatcta ggttggctta atttttcaga ttcttatctg
    12001 agtctctacg gattcacagc cttacttatt tatctagcgt attttatttt attgacgaaa
    12061 ttcaatcatg gccaaacgat tgggaaaatg atctttggta ttcgagtcat cagctttgat
    12121 gaagaagaac ttagctggtc aaccgtttta gttagagaag gtgcttgccg gttcattcta
    12181 aaatttcctt tgctgatgtt agggtatctt ccagcagtct ttaatcagaa aaagcaacat
    12241 ctgggcgatt ttttcactaa tacaagtgtt gttacgatca atctgatcaa agcgttcaac
    12301 caggaagtca acagctgatg agcaataaaa agcagtaaca gttgaaagca ccttctaaaa
    12361 gtcagatcta aaatctagct tttagagggt gcttcagtta tttttaattg tcatgctttt
    12421 tttgttttgc tactattgaa catgagctga atatgagcaa gaggaggaca ctgcgtgaag
    12481 ccgattgatt ttccagaaaa atatgagaat ttaatgcgag tggcacaaca agcactagca
    12541 aaccagcaat atcagcaggc aaaagaatta tttcaacgag cttacgagtt gaaagaaagc
    12601 tttgaggcta atagcctgct tgttttttgt ctttacgagt tggacgagaa aaaagaagca
    12661 ttgaagcaag cactcttgca tgaaaaacag tatcttcaaa atgaggagtt tgctgaattt
    12721 tattttgact tgttaatcag tacacaagat tttttatatg ctagaaaatt gatagcaagt
    12781 acagattttt atgaagcttt cgagcagcgg gtcattgaaa aaatccaatt tgcagaagaa
    12841 ttatccggac aaatggagag gcagaagatc aaggcactcc acaataaatc ggaggaactt
    12901 ccttcgttgg aacctgcacg tcagcttgcg tctattgatg agatcgaaca gcttccttat
    12961 catgagttta tccaaacagc gagtaaactt attgtatcgc cagaggtcca tattttagca
    13021 agggcaaaat tactggagac acttaggcaa ttgaacgaat gccatcctgt tttctatttg
    13081 acgattgaag aaaagttagt caaagtcata ccaaaagatc tgccaaagcc gcagcagcag
    13141 gcaagttacc agcaattatg tgtattttca gatcgttacg ggaatgaaga tgcgctgctt
    13201 agttccgtgt taaaagaaga attcacgttg caatctgcaa ttgtttatcc tgtctatgat
    13261 acatacatcg aagacccgaa gcgctggttt caactgactg tagaggcgtg tacaggaaaa
    13321 acgatgtatg aaggtacaga agatgaaaaa caagattttt taaaaaaaag agaaaaaatt
    13381 ttgcagcaaa tgatcttctt ccattagatt ttatcagtga aaaagataaa aatgttatga
    13441 aaaacaagga ttctatccat tcaatatgtt tacaatttga aagggttagt gtactattta
    13501 aaggtatgca tgaaacgttt ttgatgtata aaatgaattt ctaaactcgg agggaattaa
    13561 atgactgcaa catttgaaaa aaaaggcacg aatgatggtg ttttaaaatt tacgattgcg
    13621 caagacgaaa tccaaaaagg attaacaact gcgtttaata aagtgaaaaa aagcttgaac
    13681 gtacctggat tccgtaaagg gaaagtttct cgtcaagtat tcaaccgtat gtacggtgaa
    13741 gaagctttat atgaagatgc attgaatgct gtattaccag aagcttatga agctgctgtc
    13801 aaagaagctg gcatcgatcc tgtttctcaa ccaaaaatcg atgttgaaag catggaaaaa
    13861 ggtcaagact gggtaatcac tgctgaagta acagtgaaac cagaagtaaa attaggacaa
    13921 tataaagatc tagttgttga aaaacaagac cgtgaagtaa cagatgctga cgtggatgca
    13981 cgtatcaaac gtgaacaaga agcacaagct gaattagtta tcaaagaaga tgctgcagct
    14041 gaaaatggcg atacagtagt aatcgacttc gaaggctttg tagatggcga agcgtttgat
    14101 ggcggaaaag gcgaaaatta ctcacttgaa ttaggttcag gctcattcat tccaggtttt
    14161 gaagatcaat tagttggtca taaatcaggt gaaagtctag atgtaaatgt aactttccca
    14221 gaagattatc aagcagaaga tcttgctgga aaagaagcag tcttcaaagt gacaatccat
    14281 gaagtaaaag caaaagaagt gcctgaatta gatgacgaat tcgcaaaaga tgttgatgac
    14341 gaagtatcaa cattagacga attgaaagaa aaatatcgca aagaattagt agaaacaaaa
    14401 gaaaaagcag cggacgatgc aaaagatgaa gcagcgatcc gtatggctgt tgaaaacgca
    14461 gaaatcgttg aattgccaca cgtgatggtt catgatgaag tacatcgttc aatggacgaa
    14521 ttcttgaaca acatgcaacg tcaaggtatc tctcctgaaa tgtactatca attaactggt
    14581 tctacagaag aagacttgca taaacaattc gaaggtgaag cagaaatgcg cacaagaaca
    14641 aatcttgtga tcgaagcaat tgcagctgct gaaaatcttc aagcaacaga agaagaaatc
    14701 aatgcagaaa tccaagaatt atcagaaaca tacaacatgc caatcgaaca aatcaaacgc
    14761 gtgttgacag aagatatgct ggaacatgat gtgacaatga aaaaagcaat cgaattgatc
    14821 acaggtacag cgacagaagc gaaagaatag aaatcaagta ataaatcatt tctgaagaag
    14881 ctacttctag aaaattgttt attcgaaata agggactgtg acacgatttt gtcacagttc
    14941 cttatttttt gttaagagta aaaaatgcaa aggtataggt cagttgatcc tttgttaaac
    15001 actttttctt tttactaaac tatgttatga tagtaaaaga ctgtaaagtc tttgatagtt
    15061 tcttgcatac tttttgcaag ttaaggggtg aagaccatgt atgacaatcc aagtagcaat
    15121 gagaccgtac gctgttcttt ttgcgggaaa acccaagaag aagtgaaaaa gatcgtagct
    15181 ggtccagggg tctacatctg taatgaatgt atcgacctat gtaaagaaat cattgatgaa
    15241 gaattttatg aagaagctgt acgcgaattc actgaagtcc ctaaaccact agaaattctt
    15301 gaagtattga ataactacgt gattggacaa gatcgcgcaa aacgctcgtt agctgttgcc
    15361 gtatacaatc actataaacg tgtcaatcag caagcacaag aaaacacaga tgatgttgaa
    15421 ctgcaaaaaa gcaatatctg cttgatcgga cctacagggt ctggtaaaac atttttagca
    15481 caaacattag caaaaacatt gaatgtacct tttgcaatcg cagatgcgac aagtttgaca
    15541 gaagctggtt atgtggggga agatgtagaa aacattttgc ttaaattgct tcaatctgct
    15601 gattacaatg tggaacttgc agaaaaaggg atcatctata ttgatgagat cgataaaatt
    15661 gcgcgtaaaa gcgaaaatgt atctattact cgtgacgttt ccggcgaagg tgtccaacaa
    15721 gccttgctga aaattctaga aggaactgtt gcaagtgtgc cacctcaggg cggacgtaaa
    15781 catccacatc aagaatttat tcagattgat acgacgaaca tattgttcat cgtaggtggt
    15841 gcatttgatg gcatcgaaac gattgtgaag aatcgtatgg gcgaaaagac gattggattt
    15901 ggtacacaaa accaaaaact ttctgaagac gaaagtgtca tgcagcatat cattcctgaa
    15961 gatttactaa aattcggatt gatccctgag tttatcggac gattacctgt tatggcagct
    16021 ttagagaaat tgacaacaga tgatctggta cggatcttga ctgagccgaa aaatgcgcta
    16081 gtcaaacaat atcaaaaact tctttctcta gataataccg aacttgaatt cgaacctgaa
    16141 gcgttgagag ccattgcgaa aaaagcaatc gaacgcaata ccggtgctcg aggacttcgt
    16201 tccatcattg aagaaatcat gatggatgtg atgtttgata tcccttcaaa tgaaaacatc
    16261 gaaaaagtga ttatcacaaa agaagcagct gaattgtctg gtgagccaac agtaatctat
    16321 aaagattcag agaaaaaagc gggttgatga aaagaggctg ccgtaaaagt cattttggct
    16381 tttgcgacag ccgcttgctt tattcatcac cttgttaagg agagagacca tgaaagtcca
    16441 tcaagctgaa attatcataa gtgcagtggc accaaaacaa tatccagata cacaattgcc
    16501 agaaatagct ctggcaggac gatcaaatgt agggaaatct tcttttatca acacattgat
    16561 tgatcgaaag aatttagccc gtacttccgg taagccagga aagacccaga cattgaattt
    16621 ttatttgatc gagaacgcat tacattttgt cgatgttccg ggatatgggt atgcaaaagt
    16681 atccaaaact gagcgagcaa aatggggaaa gatgatcgag acatatttaa cgactagaga
    16741 ccagctacgt gcggtcgttt ctttagtaga tttgcgtcat aagccttctc aagaagatat
    16801 ccagatgtat caatttttga agtattatga tatccctgta attgttgttg ccactaaggc
    16861 agataagatc cctcgtggta aatggaacaa gcatgagagc gaaatcaaaa aagcgttgga
    16921 ttttgatcgc aatgacacgt ttattctctt ttcttccgtt acaaaacagg gaaaagaaga
    16981 agcgtgggca gcaatcgaac aagcaattga atcataaaaa gaataaaaaa gacttaccag
    17041 tcaaactctt tcataaaagt ttggctggta agtctttttt tgagcttaac ttacttttgt
    17101 ttcttgtctt ttttcttttc attttgtggc tgtggtttta gaaatcgatc gatctcttct
    17161 tttggtgcaa ctgttttttc cggatcgaca gcaaatgatt cgaaaagttt atctaaatta
    17221 tctgtacgtt caaatttcaa ccccattgta aacacctctt tgtttctttt tacttaatct
    17281 atcatgtaat cgtccaaatt ggtttctaag acataaagtt ttagtttttc tttatcattg
    17341 gttataatta aattataaaa agatttgaaa gaggtgagaa aatgattggc gaagataaat
    17401 tattgatgac tatccagctc acagacttaa atgaagaaga aaaaaacagg aaataagcga
    17461 ttgggctatt ttgacacgtc tgttgatcca gccaacattt atccgctctt tcacacaaca
    17521 agcggatccg tatgatcttc gaaagcttca agaaaaaata atgctagcat cggtaagaga
    17581 cgaaagttgg ttagttgtag gaaatgacga gaacgaatgt agttttcgct tggaagataa
    17641 tcaattattg ataaaaaatg tattgtctat ctctgttttc aaagaaaagc aatttttgat
    17701 tcgagacttc attcagaaaa aaatgattgc tcatggggtg ttcgcctata tgcgagctta
    17761 tagcgagttt atctatcaca atacaaaaca gatctctcaa cggctattgt ttgagaaaaa
    17821 ggatgaaatc gaacatttac ctaaaatgaa acaacaaaac ggggaagtag tagtcgattg
    17881 caaccaattt ccaggatacg atctttttta ccaaggttta tgtttcactt cgtgttggga
    17941 aatgtattat agtcgctatt atcatcaaat catccctaaa ccgatttttc tcgacatcca
    18001 acaagtagaa aaagtgaagg aattagaaaa cgaagtcata tgtatccagc tttatcgaga
    18061 cccctttaac tggcaaaagc caaacaatca aatgtttcaa tcctatttta gggaccagct
    18121 tgggtttgat catcttgctt gggacaatgg tgtcggttta ttgaaaccgc cattcgttga
    18181 atacatttac actgaccata cgatccaatc tgtccagtat cagaatgcac aaatgcaacc
    18241 tgttcctaaa aagaatgctt cattttttgt aacaaaaagt tatgatatcc aacaaggaga
    18301 ttataaagaa aggcgtgttc gcggaacact aaatgctcag gcgtattttc cttgggttga
    18361 cgagaatcga tcacgcatga tgtgctataa agttatcgat ccgactgttg ctttggataa
    18421 tggaatcgaa gcttattgtt attatattag ggaatatcta gaagtagagg taacagatga
    18481 aaaatatcaa gagtatcttt tatccttaag aatctatgtt ccttcagaat ccctgtcaga
    18541 attaccgtta aaggaaatca aacatagact ctctgatgtt accttcaaac gaataaagaa
    18601 aaagcggcga agtatc
//
LOCUS       DANKKG010000048        18302 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000048 DANKKG010000000
VERSION     DANKKG010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.048"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(42..1334)
                     /locus_tag="PW004_001379"
     CDS             complement(42..1334)
                     /locus_tag="PW004_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL2726361.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(1331..1954)
                     /locus_tag="PW004_001380"
     CDS             complement(1331..1954)
                     /locus_tag="PW004_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726362.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(1957..2793)
                     /locus_tag="PW004_001381"
     CDS             complement(1957..2793)
                     /locus_tag="PW004_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2726363.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(2810..3697)
                     /locus_tag="PW004_001382"
     CDS             complement(2810..3697)
                     /locus_tag="PW004_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2726364.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(3713..4996)
                     /locus_tag="PW004_001383"
     CDS             complement(3713..4996)
                     /locus_tag="PW004_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2726365.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(5679..6362)
                     /locus_tag="PW004_001384"
     CDS             complement(5679..6362)
                     /locus_tag="PW004_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDL2726366.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(6380..6997)
                     /locus_tag="PW004_001385"
     CDS             complement(6380..6997)
                     /locus_tag="PW004_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HDL2726367.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(7007..7831)
                     /locus_tag="PW004_001386"
     CDS             complement(7007..7831)
                     /locus_tag="PW004_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDL2726368.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(7824..8384)
                     /locus_tag="PW004_001387"
     CDS             complement(7824..8384)
                     /locus_tag="PW004_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726369.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            8558..9514
                     /locus_tag="PW004_001388"
     CDS             8558..9514
                     /locus_tag="PW004_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL2726370.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(9819..10775)
                     /locus_tag="PW004_001389"
     CDS             complement(9819..10775)
                     /locus_tag="PW004_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HDL2726371.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            10879..11481
                     /locus_tag="PW004_001390"
     CDS             10879..11481
                     /locus_tag="PW004_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDL2726372.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(11569..13068)
                     /locus_tag="PW004_001391"
     CDS             complement(11569..13068)
                     /locus_tag="PW004_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HDL2726373.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(13084..13608)
                     /locus_tag="PW004_001392"
     CDS             complement(13084..13608)
                     /locus_tag="PW004_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HDL2726374.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            13906..14655
                     /locus_tag="PW004_001393"
     CDS             13906..14655
                     /locus_tag="PW004_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDL2726375.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            14646..15431
                     /locus_tag="PW004_001394"
     CDS             14646..15431
                     /locus_tag="PW004_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HDL2726376.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            15400..16116
                     /locus_tag="PW004_001395"
     CDS             15400..16116
                     /locus_tag="PW004_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDL2726377.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(16218..17252)
                     /locus_tag="PW004_001396"
     CDS             complement(16218..17252)
                     /locus_tag="PW004_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HDL2726378.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(17256..18299)
                     /locus_tag="PW004_001397"
     CDS             complement(17256..18299)
                     /locus_tag="PW004_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017262070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HDL2726379.1"
                     /translation="MFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVGKILSK
                     VVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQT
                     TLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAK
                     NIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQPM
                     DYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTAT
                     NILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWI
                     VVLQMIHGIGLFS"
BASE COUNT         5466 a         3868 c         3225 g         5743 t
ORIGIN      
        1 gattttataa actacgggaa gtaatttccg taaatctatt cttattcttt taagctgaca
       61 agctcgtcaa tcactactgc ttttcctgaa gcaatagaat gatttgctgc gattcctgtc
      121 aaaatcgaca aaataccatc tacgtgagtt gctgcccgat tccacggatc ttcttctttt
      181 tcttcaccaa atagatcatc taataaacgc ggatctcctc cgccatgtcc gcccgcttct
      241 tgttcgacga ttacctcata tggctcaccg aacatcggat agacatggat ggactgaaga
      301 acggctgctc cttcatcctt tttatctcca cctgcattga tgtaagactt ttcgatcact
      361 ttcatttcga tccgtccttt gctgccattg aaagaaacat tgaatccttc ccacggcata
      421 tacgcgttta gcgaatacgt caaaatcgca ccagtttcat atttgaccgt tacgcctagt
      481 gtatcttcaa tagagatatc atcggaaaat acactgcggt cacgaatata tccactgtct
      541 ttttctgctt ctaaatacat ttctcgcaat tcctcattgg ctgacaggtc tatagcaaat
      601 ggatcagctt tagctagttc gcttccatag catcgactgt aaaatatttc tactccgcgt
      661 ttctccgcat tatgcatccc ataaaagttt aatccaccga atgcaaatac cttttgggga
      721 cggctgttca accaaaaatt cactagatca aaatgatggg tagatttatg aacaagaagc
      781 ccgccgctat tgtgcttatt tcgatgccat cttctgaaat aatctgcccc gtgttgcgta
      841 tttaacagcc attcgaagtg gatagaaaac acatcaccaa tcacatcact ttctatcagt
      901 tcctttatct tcgtgtgata cggtgcataa cgataattaa aagtgactcg caggcttctt
      961 cccgtctgtt tttggaccgt tattatttct tgagcttttt cctcatcgat agtcagaggt
     1021 ttttcgcaaa taacatcata tccttttttc attgcacgaa tgatataatc atgatgcgtt
     1081 cgatctactg acgtaataat gacttcagcc ggccgtacgt catccagtag ttgctcaaat
     1141 tgtgatggca tacatgtctt tacttttttc tgtccatact tgttcacaag tatccgattt
     1201 gcataatcca tccttgtttg agatacatca caaaaaccga ctagttcaca agtttgatga
     1261 tacgtagagg caattgcctc ataaaaaaaa cgcgctctcc caccaagtcc gacttgtaca
     1321 tattttttct tcatttaaag tcattctcct tttcttttaa gcaaaatact gtgacaaatg
     1381 gcagccctgg atacagtaat gcagtgagta aaaaatcatg ctgtatacac cagaacagtc
     1441 catacaaaag gcataaatac cacagaaacc ccttgacgtt ttttgacaac agccagatat
     1501 tccttttgat caaataaaca agactatctg ctttatcttc tttgcagata tcaattagcg
     1561 tactaaaaaa actatgggat acaaaaagca acagacacaa tagaaaagga atcagatacc
     1621 aagctccaat ctttcccact aataaatacc aatcaactgc aaaaataatg acaaacagct
     1681 gaaacaacgt aatgatgaaa cacatccgtt tgatttttcc tttcctccaa catacctttt
     1741 tttctgccaa gtaatggaca gatagataat aagaaggata catcatcaaa aaataaatca
     1801 gatagacaac acgagcagac agcactaatc ctaaagtgaa caaacatcct agaactaaag
     1861 cgaaaagagc attactgata agactggtta aaaaaatccg acagacatag tagttgagct
     1921 tgtaaagaaa catctcttct attggctttt tcatattcag cccttcagac cgcttgtact
     1981 gatcccctgg acaagatatt tattgaagaa taagaaaatc agaaaaaccg gtagcagcga
     2041 caacgtagac atcgcaaaca tactcgcgta agtcgtacta gatccagcgt ctgcaaataa
     2101 tttgatggca atcgataccg tgtaagatct cggcttgttt aaataaatca atggtcccat
     2161 gaagtcgtcc catttccaat aaaactggat gatgatcgtc gtgatgatcg aaggtttcag
     2221 caaaggaaaa ataattttgg taaagacact atacttgctg catccgtcaa taatcgctga
     2281 ttcatctaac tctttgggga tgctgaccat gaattgcatg atctggtaaa tgaaaaatgc
     2341 ttgtccaaaa aaatgtggta aaatcagcgg cacatacgtc ccaaccaaat tcagccgatt
     2401 gtaaataata aactgcggaa tcaaaatcac ttgtgcagga atcatcatcg ttactaacat
     2461 gacgacgaac cagaatcttt ttcctttgaa atcgattctc gataatgcgt aagcaataca
     2521 agtcgagctc agaactgtcc ctatcgtcga aactgcagag atgacaaacg aattcctaaa
     2581 gaaagtaaaa aatgaaattc cagcaaaccc cgcccatcct tttgcgtaat tttcccattg
     2641 aaaagtctct ggaatcaaat tcagcgaacc actcataatc tcttgatccg ttttaaaaga
     2701 acctgcgatc atccataaga ccggataaat catgactaga cctaatagca aaataaaacc
     2761 gtattgaaac catttcgtta ctgtattatt catttcttcc tcctctcatt cacacaacga
     2821 cccatttttt tgatgatttg aaaatcagaa aggtgacagc tcccataatc aacagcatga
     2881 tccacgaaat agcactagca taccccatag aattaaaaga aaaagcttgg ttatacacat
     2941 aaagagcata aaagttcgtg gcatcattcg gaccaccttg ggtaatgata aatgcctgcg
     3001 taaaagtcat aaacgccgcg attgtctgca taaccaagtt atacaaaatg atcggcgaaa
     3061 gaagaggtaa cgtgatcttg taaaacgtct gccatttgtt cgctccatcg atctctgccg
     3121 cttcataata cgagcctggg atctctttga tacctgcagc aaagatgatc attgccgaac
     3181 cgaattgcca agcagacatc aaaacaagag ggatcatgac cagtttttga tcgctaaacc
     3241 aggaaagcgc aggtgcgcct aatcgttcca ggaaaatatt cactaacccg tttttagaaa
     3301 acaactcccg ccaaactaga gcgatagaaa tactgccacc gatcaaacta ggcacataat
     3361 acaaagaacg aaagaaagtc gttcctttga tattcctcgt taaaagataa gcaatcaata
     3421 gaccaaagat cagtttcaca ggtacagatg caaatacata gagaaaagtg actcggatgg
     3481 agttcaaaaa acgggcatca ctcagcatct ttatataatt atccaagccg atccatgaga
     3541 ctggatttcc caaacgataa tcggtgaacg aataataaag tgacaagatc atcggtatca
     3601 ctgttatcaa tagaaatcct aggatgaatg gagaagcaaa tacgtaaccg accacattgt
     3661 cttgatccaa gaaccgtttt agtttttctt ttttcatgcc ttcctcctgc tgctatccta
     3721 gaatgtttga cgcaaactca taaagactct ctgctgcttc ttctgctgtt acttcttcat
     3781 aaatgacttt ttcaactaaa agattgtatt ggtcttgaat ttccggattc aaagcactat
     3841 caaagacatt cacttcgccg ttgtccatat cggaaatcat ttctaaataa tcaaatgtcg
     3901 cttgcaaagt tgcatcatct gcacgagcga gctgttctcg aatattcgac atgattggta
     3961 caccacgttc tccttttaaa atttcattgg cttcttcact gttttgaaag aaatcaataa
     4021 acttagcagc ttcttcttta tgggctgagc tttttgccat actcagcatt tgcgaagaac
     4081 gtaaagggat accagattct ccatctgctt ttctgcgagg tggattaatc aactgaatct
     4141 ccatatcttc aggagcccct gacaatattt ctgtcaactg gttacttgat agaaacacca
     4201 ttgccgcttc tctatcccta accgcctgaa aagacagatc cgtcgtactt gcagctccac
     4261 cacctggttc tggatgggcc cctttgtcta ccagattttt acgcagctgg aaaaaatctg
     4321 ttagatagtc tggattttca aatcccaagc tgttagatag atcttcccga ttataaaaat
     4381 tcaatccttt ctcaacttgt ggtatttgaa taatacagcc agctataaaa tcttcgaatc
     4441 ttgaaatagc atagattcct aattcttcag tgatttgata gctgatttgt tcaaattcat
     4501 cccatgtcca atttgaagct ggttcaggga tatttaattg ttcaaatata tttttattgt
     4561 aagcaatcgc aaacgaattc accccgttag acagtccaac taattgcccg tcgtaagtcg
     4621 tcgttgctaa ataggattca tctgcattag aaatatcaat tatcccttgc tccacatatt
     4681 catctaatgg ttcaatctga ttgatgtatt gaggaaattg attgttcagt tggaaaatgt
     4741 cccagatatc atctgatgct gctagtgtac ctaattttgt gtaatatcct tcataatcat
     4801 aaaattcata atcgatcgtc acatggggat ttttttcttc ataaagctcg attactttta
     4861 ctgttgcatc gtgtcggact tgcgaacccc accaagccat gttcagcgtg atttttttgt
     4921 catcttcttg cccacaagcc gtcaagaaga ataacataac acttaaagct aagatgattc
     4981 tcattctttt cttcattctc tgcacctcta cttccattgt tatttttcaa tatactgtat
     5041 ttttatacag aacatattta ttaaacgatc tttttcaagg ggaataaaaa ccaagacttg
     5101 ccttatcgtt ctgcctagga tgaacaactc aatactttca agaaaaatta gatagtgctg
     5161 attcttcttt catcgctaaa aacacttctt gcttcaatga tgggaggata attacgcaaa
     5221 gaaaaaagga tatcagacaa ctgcccaata tccttgacat agctaaataa gcatgcttct
     5281 tcgtgtgcgc tcttccctaa aaaacagaaa aataactagt aattctgtat gaaattaggt
     5341 acaatataca cgtcgatgca caagcattga ctatggcaac ttgattggtc agttcccgct
     5401 gacttttcaa gccccgacaa tttagctgct actaaattgt cgggtgcctt ttttagtttt
     5461 tattcgtttt tcattctata gaaagcgctt caataaatca agacattttt ttgttagaat
     5521 gctaataata acaaaaggtt ttaactcttc ttatgtacat tacaggtaaa aaaacaaaaa
     5581 aatttctctt ttttttgaat tctgaacaaa tagtactgga gtacaaaaga cgaggaagag
     5641 ccactatctg ctcttcctcg tcttttgatt ctcacctgtt attcatgatt ctttttctca
     5701 gtttcccgat atttttccat tttacgacta aaaagctctc cgctacttgg tggcgtatat
     5761 gtgatggcat ttgctcctgc ttgaatggtc tcagcaatac tttcttccgt tggaccacct
     5821 gtcgcaatga taggaagttc aggatatttt ttacgaaaat ggcgtactgc ttctgcagtt
     5881 tctttacctg cactgatatt gataatcgaa ataccagctg ctaacttttc atctatttct
     5941 gtatatttcg acgttaccgt actgataact ggaatatcaa ctactcgaca aacttctttt
     6001 actgtctcta aaggcgtagg accgttcaaa acgaccccta acgaaccttg cgcttctgcg
     6061 aacatgctca tgtaggaaga acgagcacct tgtgtcaaac caccacctac accggagaaa
     6121 actggaatgt cagctgcctc aataatgctt ttagtaatcg ctggatgagg tgtaaaagga
     6181 tacacagcaa ttactgcatt cgcatttgta ttacgtatga tcgcaatatc cgtagtaaag
     6241 atgatcgaac ggatcaattt tccaaatatc ttgatcccgc ttgcttctaa aatgatttcc
     6301 ggtactctta cgatatcttt tctcaattcc gtcatgattt ctggcgtaaa ttgttctgcc
     6361 atcagccact tctcctttgc tatctccggt catactgatt caattgatac gtctcgatga
     6421 cctggatgat tttttgcgca taaccggggt ctgtcgcata acctgcatct tgcaatgctt
     6481 gtgcggcttc ctgatagttg gtggccgtaa tcactttttc atacagcgca ggattccaat
     6541 ctactccttg gacgaacagt tgtgtatggt catccatgga ctgttcccat gaatcataca
     6601 ctttgaagtc tccttggata gttatccatt cttcgttaac aaattccttt gtttccaggc
     6661 ttactttctt ctgatcccca taagccttga tcccgaaaag attattgtat ttgctagcca
     6721 aggtactttt tccccaattt gattctaata tagcttgtcc taaaatgata ctaggaagca
     6781 ctccataacc atcttgtagt tcttgggcat gcggaacaag ccgttcaata aattgctgat
     6841 gggattgcgc ttcattttct tgctgattat cagtaaaagg agaagacaga ttcttcaagg
     6901 aaaaaacaaa ggctaagagt atcactgcta caccggcaac cactgctggc caacggatat
     6961 tcttgcgctt ttgctttttc cacttttttt tcgccatgat caattcctac ttttcattta
     7021 attcattcag atattcttct agcgtcaatt ggtgtttggt gatatactca gcgttttctt
     7081 taccgacata acgtaagtgc caaggttcat atgtgatctt agttatatct tcgtgtcctt
     7141 ttggataacg aacgataaat ccatactgcg cagcattatc tgcgatccat ttagctccag
     7201 gctgatctcc gtaacttgca tccaatacag tagaaggata actgttgtac cagttttgat
     7261 cgacgacatc gactgccaga cctgtgtggt gttcgctgta tcctggctcc gtaatcgttt
     7321 ctttagtaat ctttgtcgcc tcctcttctg atacaccttg acgactcatc acatcttgga
     7381 cattttgttc gaaaacttgt tgttgggatg atacagaccg gtaagctgag accatcacta
     7441 atggaaatcc agctgcttca gcagccttgg caaatgcttc atattctgga gcaatgcgtt
     7501 tgtcgatcaa gtatccgtta cttaatgatg ccaattgagt agactcatca atttcttttt
     7561 ctaatttgtg atctggacca accaaaacta gcttccaatc gtctgtttta acatctggca
     7621 attgtttgga cgtacgatct tttgtttctt tgtcctttac cttgctgctg gttgtttgac
     7681 tgttcttttc tttactagca gttgttttgg tgcttgttcg ttcgactgct tgattgccgg
     7741 cttgaacatg cgaatcatca tcgctaaaaa taacatagcc cattgctgcg atcataatca
     7801 caactgctcc aaatcctaaa aatttattca cttgaaaaat cctttcgttg ttcttcacgt
     7861 tccccattcg cctcatgaac gtaagtttct tccagatttt cattcaccca atcgagaagt
     7921 tcgatttttt ctccttcgat ctgtttttga acatctgctg gcagacgaaa attcaaaata
     7981 tcatcatagc cccattcatc atattgaata cttactttca agtcaaggta tctaccagcc
     8041 tgctgattat ttttcaaagg gatcagctcg acttttgctg ctttgctgct aagccatttt
     8101 tgcgcgatgc gtgttttgat ttgcttgtac tctgcaacgg caacgtttcg tttttcggga
     8161 aaaataatcg tttgacgcaa aatcttttgc gttagcatcc attcataatc agtgaaaaga
     8221 ttttttactt tctgcatcgc ttcagatggc agatttttct cgccatcttt ccatttttgc
     8281 cattgttctg gactgatcag caagtattca tctaaaaaat ttttttcagt ttcaaactgt
     8341 tctaataata cactcataat aagatcaata tatacttgat gcacgtttat cccctccttt
     8401 taactattct acttaagttt tgatcaaaaa gcacctctta aatcgctgct ttcatttttt
     8461 ttaatgtttc aagtcctctc ctctttattc ttcgtgattt ttgttataat aatagacaga
     8521 caagaaactc aatccaacta tgaaggagcg ttttcagatg aaaaaatatc aatggggaat
     8581 cgttggtctt ggtaccatcg cacatgagtt tgccgaaagc ttcaatcaag agacgagcga
     8641 actaacagca gttgcttcca gaacttcaga aaaagcggaa aacttcgctc accgttacaa
     8701 tattccaaaa gcttacggaa gctatcaaga aatgttagac gatgctgaaa tcgacatcgt
     8761 gtacattgct gtaccaaata gacagcatat cgaccatatc ttggcagcat tggaagcagg
     8821 caaacacgtt ctatgtgaaa aagccatcac gatgaataaa aaagaactgg ctgacgctat
     8881 gcgcttagca gaagaaaaga atttgatctt agcagaagcg atgacaatct tcaacatgcc
     8941 actttatcag cagttacgct caataatgga tacaggaaaa ctgggggctt taaaaatgat
     9001 ccaagcacct ttcggcagtt acaaagaacc agaccctaaa aaccggttct tcaatccaga
     9061 attagctgga ggagctctgt tggatatcgg tacgtatgct gtatctttcg ctcgtttctt
     9121 tttgagctct caacctgaag tagttgcctc tacaatggtt ccttttgaaa caggcgtgga
     9181 cgaacaatcc gtcactatct tgcgtaacaa agaaaatgaa cttgctgctg tcagcttgac
     9241 ttttcaggca aaaatgccaa aagtcggtat cgttgccttt gagaacggtt atgtgacgat
     9301 tgctgattac ccgcgtgctg atcgtgcgga gatcttattt acagatggta caaaggaatt
     9361 catcgaaagc gggaatacat ctgaagcaat gaattacgaa ataaagaata tggtacacat
     9421 gattgaaggt gacttgccca atcgctctct cttcttgaca aaagatgtca tcgaaatctt
     9481 ggatcaaatg caagttctat ggaaaaatat gtaattttaa aaggttgtga aaaagctgtt
     9541 ctgcatcaag taataagaac agccaaacaa aaatagctcc tcctattttt cgtttggctg
     9601 gacttattac cgcagaggaa agcttttgaa caccgtttaa gaaagaggtt gtgagcctac
     9661 cgttcagctc cgagtcacaa ggagcaattt caaaaaacag cttctcatgt ttgttgaata
     9721 ttgtgacttg tgaccgagga gttgacagga gaacaccgtt taaagaagag gttgtgaaat
     9781 cagctttttg acctgaatat tagctgaatt cacaacctct atttttggat tttttgttgg
     9841 gagaattctg ccttgatttc aataaaactt ttaaaagact gcaaaaactg gaacaattct
     9901 gctcgatcct cgaattcagg gcgactagtc ggcagaggca tttgccgata caatacataa
     9961 acttcttcaa tacgtgttaa aatttgtctg ccatcatttg actctgaaaa tgtttctgca
    10021 gtataaatca ataaaccata gatatgtttt ccataataag ctggttgctg gattctctct
    10081 agattttcca gcatgtcttt aatcacattg gcctgtgctc ttctcataga gaagtaagtt
    10141 tcgtagtata gcggctgtct gagccagtag ttttcttgat gttctctggc atcttcttgg
    10201 ctttcgcgaa tgaatgtcag taaatgttcg catttgatct gcacatcttc tttattttct
    10261 gataaaagaa attctgccat ttgtctcaaa atatttcgaa attctttttc gatctgtatt
    10321 tggttttcct tcagctgctt cgtacgatct ggcatgtaaa catttgcaag caaagcaaat
    10381 actaatccaa tcgccattaa tcccgcttca ttgccgatca gctgccaaga catgttttcc
    10441 tccactaaat aatgagtgac caaaaccgaa tttactacta tcccttcggt taatttgaag
    10501 cgagaggcaa gcgggatgaa caggaataaa aaaagactga agccaaaaat agtataccca
    10561 acagcaaaaa aactgaacag tgctgttgcc gtagctaata taaaggctgc gattcgattc
    10621 atgcccgtac gcaatgtaga gcgcttggta tttcctacac ttaataatgc aatgatccct
    10681 gcagctggcc aatacaacag cgataagcta cccgctgtga ccatcgaaaa gaagacactg
    10741 atcactgttt tgactgttcg cattcctata cgcatttact tccccctttt ttattttaat
    10801 tatacttgaa taatgaaaaa ctagctatca gacaaaagtt taggtatgat agtaaaaaga
    10861 ccaaaaagga gaatctctat gcaatttggc cgttttcgac taggtatgcg tacgatcaaa
    10921 agtgccttag ctgttttttt atgtatcttg ttttttcatt tgactcaacg aggaatcccg
    10981 atgattgctg cattatctgc tgttttttct ttgcgtcagg acttgactac aaccgtttct
    11041 tttggaaagt ctaggatcat cggcaataca ttaggtggta cattagctat catttatttt
    11101 tttgtcaaaa gctattttca caatgatttt ttggtagaat tgatcttgct tcctattcta
    11161 gttattattg tgatcgtcgt gtcagatggt attaacaata attccggtat catttccgca
    11221 atagctacgt tgttgctgat tgcgttgagc atccctcaag gcgaatccgc tttgtatgct
    11281 atccaacgtg tactagatac ttttataggg acattcattg caatcggtat caatttcttt
    11341 cttcgtcctc ctgaaacaga aaagaaggaa gaactatcag aagatttggt cgaactccaa
    11401 aagaaagaac gattgctaag aacaaattta caagagatag aagctaagat ccaaaaacaa
    11461 aagaaaaata gcgaggacta attttctttt gcaataaaat aaaaaaatgg agagaagaac
    11521 tttaaaaagt cttcctccat ttttgattga acggtgaagt gtactacttt attcttcctt
    11581 catcgagttt tgttctgcct cttttggcgc ttcatacata tttacttggc tagtagtccc
    11641 acgtgtcgac aaattctcta cgatttcctt caagtcaagt cctgttgtcg cttttagtgt
    11701 ttcttgagta gaagaaagca aatttgtggc ataatttgtc actcggttcg ctccactggt
    11761 ttctcctgaa ctgcttcctg tatcgacaac agagattttt tcgatatttc ctagcggctg
    11821 tgctgcttcg cgcatcaatt gcggtagcat atcgataacc atactcaaga cagctgcttc
    11881 gccatattcc ttgaaggcat tggcgatctt ttgttttgct tcggcttctg cttttccttt
    11941 tgctagtgct gcttcggctt ctgcttgtcc agctaaacgt gtcttattgg cttccgcttc
    12001 tgccagtgct tccacacgga atcgttctgc ttcggcttca gtcacttctt tgactttttg
    12061 agcttgtgct tcttgttctt tagcgtaccg atcggcatct gcttttttct tcacttccga
    12121 atcatattgc ttttcacgac gtgtgatttc tttttcttcc agttcgattt gtttttgacg
    12181 ttctacgatt ttaatctgca tttcctgttc gattacttgt tgttgtgctc gggcactttc
    12241 taaattgtag gcttggtctg cttttgcttt agcaacatct tgttcttgtt tgtaggttgc
    12301 taatttcagt tctttttctt ttaatgattc tgcgatctct gtttgtcgct gtaattcagc
    12361 tgcttgtgat tctttttctg cttgagcttt tttgatacgc gtttctttta gagcttccgc
    12421 ttctgctata tctgcatctc ttttgacttg ggcgattctt ggtttcccta aagagtcaag
    12481 ataaccgttt ttgtctctga cctctttgat agtgaaagaa acgatgatca accccatttt
    12541 tgcaaggtct acagaagcga cttcttgtac gctttgactg aacttatcgc gattttgata
    12601 gatttcttct acagtcatag aaccaagaat cgagcgcaag tgtccttcta atacttctct
    12661 tgcttcattt tccagttctt ctcttgtctt gcctaaaaac tgttctgctg ctgtagcaat
    12721 ctcttctaca gatgaaccga ttttgatgat cgaagtacca tcacacatca ccggtacgcc
    12781 ttgttccgta tacacttcag gggtggagac gtccaattta ctcgataata gactgatccg
    12841 attcgaacgt tgaaaaactg gcaagacgaa tgcgccacct ccacgcacaa ttttgatttt
    12901 gttattgctt tcatcaatgt gcacattttt gcttcctaaa taacttccac tgatgatcaa
    12961 cgcttcatcc ggttttgctg tctggtactt cgctacaaag acgatcagca acatcagtaa
    13021 aatcaaagct gcgattgcga tcgcgataaa aacaactggt atttccattt aacacacgct
    13081 cctttaagct tttatcatgc tttcataagg catgacataa cagattcttt ccttgatctc
    13141 aatgatcaat actttcgttc cccgctcagc tgtctgttct tgggggctgt aaaaacaagc
    13201 tggacgacta atggaaccgg taacactttt caactgaatt tctcccatac ctcgtacagg
    13261 gatcggtgtg atcacaacag ctacttgtcc ttccagagtt ttttcagaag acgacatcgt
    13321 tgattcggca tgtttcatcg gcatcaatat atagaaatta agcaaaaata ccaacactat
    13381 cgatatgcct ccactagcaa taaataaaaa taaagatgat tgatctgtta aacgctcacc
    13441 tagaaaacca aataagcttg tgaaagttaa ccaagggata ataagcatcg gatcaagcgg
    13501 gccatcaaaa tcgaatacat ctccaaaaat aatcaatagg actgccaatc ctgcacaaat
    13561 cagcaatgta taccaataaa tcgtctcaag tgcgattccc cctatcatat acacgcctcc
    13621 ttccatttct acgtctatca taccaatgtt tgaaaattct gtatagcttc ttttaccgac
    13681 tcagtggtgt tttttatagg aaggttgtgt ttatcgttga caatttgtta aatttttgtt
    13741 ttcatgttaa aattttcaca ataaattctt tcgaggtagc ctatgtttcc tttttttaag
    13801 aaaaaaccat taaagctcca gaacacgatc atccttgtcg tttgtttctc cacattcgtt
    13861 tctttgattg tttctgccat attgatcagg aattttattc tttcgatgaa ccgatctagt
    13921 gtgtcagata aaatctctag cattgctaaa atcgttgcca atgataaact catccaaaaa
    13981 aagctgcaga ctggtgaccc gacacatgcg attcaaggaa taacaaatga aatcatgatt
    14041 gcatcgaatt tagattttat cgttgtcatg gaccctgaag gcattcgata ttctcacgca
    14101 gacccaagtt acatcggtct tcctttctca agtcatgaag atgtgaaaaa agtgattgga
    14161 ggacaagaat ttttctctga aaaagaagga atgttagggt tcggcttccg ctatttcgtc
    14221 cctatttatt atgatcaaga gattattggt gcagtttgtg ttggcttaac aaacaacacg
    14281 attgacaatc aagtcaaaga tggacaaaaa atgattgttt tttcattgat tattggactt
    14341 agcgtaggca tcataggcgc cgtttttctt gcccaaaaaa tgaaaaaagt actgcttgga
    14401 cttgaaccac aagaaatagc agaacagtta agtgaaaaaa cgattatcga aaatgaagta
    14461 gatgaaggaa tcttagccat ctcttctgat aaaactatcc tcttgatcaa tacacctgca
    14521 aaaaacattt tctaccaaac agagcagcta ttggatttcg aagtaggaaa aaaatcagtg
    14581 ataatctata tgacgttttg tttaaagaga cgattgagca tcaaacgacc gttcggactc
    14641 aatccatgta tttaaatggg atagaaacta tttctagcgt tacacctctt tttagcaatg
    14701 accgtttttt aggagcagtc gcaactttac gaaatcgatc agaagtaatg aaattaatgg
    14761 aggagcttgg ggggacgaaa caatatataa actccatgag agctcaaacc catgaattca
    14821 tgaataaact tcatgtcatt tctggtttga ttgatttgaa gaagtatgaa gaagtcaccc
    14881 attttatcca gcagctaaat caaaattata aaaaagagat tggccaagtc tcaagtctga
    14941 tcaaagtacc tgcaattgcc ggttttttat taggcaaaat aaacgaagcc aaagaacaat
    15001 ctgttgaaat gctgttagaa agcgattctt ttatccctaa aatggagatg gatgactcca
    15061 tccattcact tttgcaagtg ttagggaact tacttgataa tgcaaaagaa gccgtgctac
    15121 aaaaacatga aagaaacgga caggtccgga ttcatttaaa ctatgacaca gaagctgaag
    15181 tattcatcgt gaccgtctgc gatgacggtg tcgggatatc agaagaaatc tcagaaaaaa
    15241 tatttacgct aggtttttcg acgaagggaa tcaatcgtgg gtttggattg aatctcatcc
    15301 attcaatcgt caaacgccac ggtggatttt tagaattttc caaacaaaca aatggcggta
    15361 caatcgtcta tctagaatta ccaaaaaagg aagatcaaaa tgatgacaaa tgtcctgata
    15421 atcgaagatg atccaatggt tgcagaattg aatcgtactt ttgtggaaag catccctaat
    15481 atgaacgtaa tcgaaagttt cagaaatgta gaagaaggaa aaaaatattt cagacaaaaa
    15541 aacgcagatc tgctgctgct tgatgtatac atgcctggcg aaagtggtat ccatttttta
    15601 tcttggctta gacagcaagg aaatgatatc ccagtgattt tgatcactgc tgcagatgat
    15661 atcgaaacca taaagaatgt gatccgctac ggtgccttag attatttgat caaaccgttc
    15721 actttgcaac gttttcagct ggcgatcgaa aagtatcttg ctttctctca gacaatccaa
    15781 acgactgaaa aagccgatca gaaattgatc gatcaagtat tcaaccacac cactcccatt
    15841 cacgaaatac catcattcat cgatttgcct aaaggtctgt caaaactgac tttaaaaaaa
    15901 ataagtgcat ttattctaaa agaagatact attttttcaa caaaagaact ggctcaaaaa
    15961 agctcactgt cacgtatatc cgtcaagaaa tatttgaatt ttttagtaga acaagatata
    16021 ttgaacgaaa gaatcaaata tttggaaatc ggccggccgc taacctgtta tgaattgaca
    16081 aagaatgggc ggaaaaagct cacagattac tgttgagtca tcgaattatc ccatacaaaa
    16141 tagtccaagt atacagaagt aacaactgtt tacttggact atttttaaac attttattga
    16201 cgaatatcca tgttttctta tcttaattga cgggctttag gaagagatac ctcatttttc
    16261 gtttttccag tttctattat ttcacgaaag ttctcgaaac ttgtcgaaat catatttgat
    16321 aatgcctcat ctgtatttga acctacatgc ggggttacaa gaactctagg gtacatttct
    16381 actaattgct ggatagcagg gtctggaatc gtctcccatg gcttgaagga tcggaaaaaa
    16441 acctctgttt catttgcaaa aacatcggtt gcaaaaccag ctaaatgatt gcttttcaaa
    16501 gctcttaaaa tagcttcatt atcttgaagc tctccacggg ctgtattaat caagatgcta
    16561 ccttttttca tttgagctaa aaatccttca ttgatcattt gatcgttttc acctgggata
    16621 tgaggtacat gaatactgac aatatcgctt ttggctaata actcttcaag cgagtggaac
    16681 gtcaatactt ttttagcagc ttctgtctga tgaatatcgt agccgatcac tttggctcct
    16741 agtcccttaa acaaaacagc ttccgtcaac cctatcctgc ctgttcctat gatccctact
    16801 gtgcaatttc tgatttcacg actaaacatt tgctcatcca ctcgaaaatc tttgaaagat
    16861 gttcggatcg tcgtatacgc agtatttctc aaaagcatca tggcaaaagt caaagataac
    16921 tctgctatcg catttggcga atacgatggt acacgggcta ctgtcatacc aaaatcagct
    16981 gccgcttgta gatcgatatg attgactccc acagtccgtg taaaaacata acgaatccca
    17041 tattcctgca tttttgcaag attggtctca tctgctatac aatttcccct caacaataca
    17101 gcatcatggt cctttgctga cacgatattg tcatgtgtca acagttcttc ataaagactg
    17161 agatcataat ggtaaatatt caatttttca aaaaaatcta tctcgttcgg acgaacaccg
    17221 taacatgcga ttctcatact ttcttcctcc atttcttatg aaaataaacc tatgccatga
    17281 atcatttgta atacaacgat ccatacaggt gtcagtacca cagcaaaaat agttgacagt
    17341 aaggatgcat tggaggctaa aagtgcctcc ctatcaaaac tgatagcata ggctgctgcc
    17401 actgttgcag ctggtgtagc catcatgata acgattgttg ccagtgcttc aaaattgact
    17461 ggtagaatat tcgttgccgt caaaactgct agaatcacga tattcaatgc aggtacaaat
    17521 accactttga ctagactgta ataccatgaa gttttttcag atgcagcttt tttgaagctt
    17581 acttcaccta atgttgcgcc gattgctagc cacgccaatg gtgaacttaa accagctaaa
    17641 taatccatcg gctgaaatag ccacggtgca gtctggtcaa ttcgcaaaaa cgctacttgg
    17701 cttatctctc cttctgcacc tgtaactgac acttgtggca gatacccttg gaacagccaa
    17761 atgaaaaggc cggcaaaagt agcgataatg attggattga cgaacatcgc tttgatattt
    17821 ttagcttcca tcttcaagcc actcattttt atgtatccat aagaatacaa aaagattcgg
    17881 tagccaatat tgaaaattga ggaatacatc acgccgacag gaccataaat cgcactgacg
    17941 ataggcgttc cgaaaaaggt cgtagaacca aagatcgtca ggacacgcaa cgttgtttgc
    18001 ttatcccctt tatagcttaa gaaaaaaggt tttgaaacta gaataagaat aatataaata
    18061 actaatcccc aaatcaagac attgattcct tgcatcagta attctgtatt gatatcttgc
    18121 atgaaagctc gaaatgccag tgctggcaaa gccactgtta agacgacttt cgatagaatt
    18181 tttccaacag attcagaaaa aatccccttt tttctacaaa caaaccctaa taatataata
    18241 aaaactgttg aagtgattgc actgacaatc gacatatctg tcaaagtcga tcgaaaaatg
    18301 tc
//
LOCUS       DANKKG010000049        17971 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000049 DANKKG010000000
VERSION     DANKKG010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17971
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.049"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(383..1849)
                     /locus_tag="PW004_001398"
     CDS             complement(383..1849)
                     /locus_tag="PW004_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2726380.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(2140..4224)
                     /gene="cadA"
                     /locus_tag="PW004_001399"
     CDS             complement(2140..4224)
                     /gene="cadA"
                     /locus_tag="PW004_001399"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HDL2726381.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
     gene            complement(4749..5108)
                     /locus_tag="PW004_001400"
     CDS             complement(4749..5108)
                     /locus_tag="PW004_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HDL2726382.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(5138..5479)
                     /locus_tag="PW004_001401"
     CDS             complement(5138..5479)
                     /locus_tag="PW004_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726383.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(5476..6150)
                     /locus_tag="PW004_001402"
     CDS             complement(5476..6150)
                     /locus_tag="PW004_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDL2726384.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(7326..8624)
                     /gene="asnS"
                     /locus_tag="PW004_001403"
     CDS             complement(7326..8624)
                     /gene="asnS"
                     /locus_tag="PW004_001403"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HDL2726385.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(8664..9854)
                     /locus_tag="PW004_001404"
     CDS             complement(8664..9854)
                     /locus_tag="PW004_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDL2726386.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(9875..10402)
                     /locus_tag="PW004_001405"
     CDS             complement(9875..10402)
                     /locus_tag="PW004_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HDL2726387.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(10449..13238)
                     /locus_tag="PW004_001406"
     CDS             complement(10449..13238)
                     /locus_tag="PW004_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDL2726388.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(13385..13585)
                     /locus_tag="PW004_001407"
     CDS             complement(13385..13585)
                     /locus_tag="PW004_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL2726389.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(14038..14358)
                     /locus_tag="PW004_001408"
     CDS             complement(14038..14358)
                     /locus_tag="PW004_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726390.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(14861..15979)
                     /locus_tag="PW004_001409"
     CDS             complement(14861..15979)
                     /locus_tag="PW004_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726391.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            16450..17757
                     /locus_tag="PW004_001410"
     CDS             16450..17757
                     /locus_tag="PW004_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDL2726392.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
BASE COUNT         5175 a         3807 c         3121 g         5868 t
ORIGIN      
        1 aatggtgttc aatcagctga ccttcagcat tagctcaaaa agcggcaaaa tatgaggagc
       61 tatttttccg tttgtttctc taatactcag gtcaaatcgc tgattttaca acctcttcct
      121 taaacggtgt tccaaagctt gcatctgcgg taataagccc agccaaacga aaaatatgag
      181 gggctatttt tgtttggctg ttcttattac ttgatgcagg acagcttttt cacaacctct
      241 gattcacggt gttccagaag tagcttcttc gaaaaacaag ccgaaacttc acaaaaattt
      301 gagaagcaat tttcgtaaat tccttcttat ttcttgaagc tgaccacttc tgtcacaacc
      361 tcttgtaaca agtatttcta ttttaaggct gaaccactgt ttctggttca gtgatttctt
      421 tgtttttgtc tcgacgagca attgctggca agatcatacc taataagatc aagacgattg
      481 gtgtgatgat attcgtaaat aattggaacc accatgcttt aggatcagca gtataatcta
      541 cttttggcac cattcctagg atacatgcaa acgcagtgaa ggcaaagcac caagcaccaa
      601 agataaaacc gatttttgga tttttaacaa atttatattc agaattgaat ttcttataag
      661 ctttattcaa catcatatat gccaagaaca cccataggta acgcattggc atgacaactg
      721 agttcaagtt cgtcaaccat tttactaatt cattcatatc tttgatacca aaaatcggca
      781 acaaaataat gattcctact agtatacctg tcagaagata accattcacc aatgtacctt
      841 tacgattacg tttacgtagc cagtttggta caaattctgg atccgcatct gctaacaaga
      901 tttttagtgg tgcatcaatt gaaaaggcta atgcggagat ttgtccaatc atgtttgtca
      961 atgcgtaaat gatcatcaat gagttaccga taccatagta attacctaaa atttgaaaag
     1021 cactgtatgc cccatttgcc atcaagtctt taggaatatt atcactagca aacagcatac
     1081 ccattgcgac tgagcctaat acagcacaaa tccctaccat tgctgctaag aagatcatcc
     1141 ctttaggaaa ttcctttgca ggatttcttg tagagttgac ataaggggat attttttccg
     1201 cccctccaac tgcaaatact agcatcgaaa cggttgtgaa ataactgaaa tcaaatgttg
     1261 gaatatatgt tttcacttga ttcatatgtg gtgtggcaaa ttccatcgtt ggattgatga
     1321 acggtgccgt tacagctaaa agaataaata agatcgacat gacgaacatc gctgttccgg
     1381 ccagtccgcc aatcactttc aaagtagaaa gtccttttgt tgataaatat aaaaaggcaa
     1441 gaaatataat aagtgaaata actgctacca tttgtactga catcgtattt acgatatttc
     1501 catttccttg gactgcccaa ccaccagcaa tcagaattgc ttgaggcttt tgtgccaaat
     1561 aaggaatatg gacgacccag taagtccaag cggcatagta tgccagtcgt ttattagatg
     1621 tcgtattttc gacccatgaa ctgacgcctc ctttactatc cttgaaggct gatcctaatt
     1681 gtccaacgat caatgcgtat ggaataaaat aaatggccaa aatcaatagc caagaggtga
     1741 caactgatac tccttgctga gcatagttat taactacatt ccccatgccc caaaccatgt
     1801 taaatgcaat taacgcaaca gtaaaccagc gtaattgttt gttgttcatt cgttctctcc
     1861 tttatgataa aaatttgtta tccagatgta atcatattca atttttaaca aacttacaag
     1921 acagtgttat tttatgtgtg tattctttta tttaatacac atcgtttttt catatttttt
     1981 ccatcaaaaa aataagttat tttcttttat ttcattgacc ttattgaatt tcaagctatt
     2041 ttgaattttc tgcaaaatta aaaacattag ttattttata atcctatcta ttttctttac
     2101 atttttcatt aagaaaagga tataaaaaaa agaggacgtt cattcgtcct ctttttttac
     2161 atgcaagctt cgcatggcat tcaaaacagc taatactgtc accccaacat ctgcaaatac
     2221 tgcatctccc attgatgcaa aaccaaaagc acctaaggca agtaccaaaa tttttatccc
     2281 aatagcaaag acaatgtttt gtttgacgat tttcaatgtt ttctttgcta aatggattgc
     2341 ttctgcaatc ttcgatggtt gatcattcat gatgactaca tctgctgctt caattgcagc
     2401 atcgcttccc aatccaccca ttgcgattcc tacatctgct cttgctagca ccggtgcatc
     2461 gttgattcca tctccgacaa atgcggtctt tttattgttt ctttgtaaga tttcctctaa
     2521 ccgctgcact ttatcttcag gaagcaattc gctatatatc ttgtccacgc ctacttgctt
     2581 cccgatatga tcagcaattt ttttcgagtc gcccgtcaac atgactgtgt tcttcacacc
     2641 agcctgtttt aattgaacca atgcgtctac ggcatcttct ttcaaggtat ctgcaataac
     2701 aagataacca ctgtaacttt gatcgatcgc tagataaagc aatgtaccga tttcttgact
     2761 taaaggaaaa gagatttcaa atctttccat caatttgtga ttacctgcta atacttgttt
     2821 gccatcaact tcagctaaga tcccttcacc tgcaaattcc tggatattag ttgttggtaa
     2881 aagcggacct gcgtagcctt ctaaaacaga ttgagcaatc ggatgtgtag aaaactgttc
     2941 tgcactcgca atatattgca tgaatttctt tggatcgatt tttgtatcga tcgtctggat
     3001 cgaaaagtca cctttcgtta atgtgccggt tttatcgaat accactgtct cggtttgcgc
     3061 aagcaactcc aagtaatttg aaccttttac taatacaccg attttacttg cgcctccgat
     3121 accgccaaag aaactaagtg gcacggaaat cactaatgca catggacatg agatcactaa
     3181 aaatgtcaat gctcgataga tccattcaga aaacgccgct ccagaaacaa caagtggagg
     3241 taaaacagct aataaaacgg ccaatcctac aacgactggt gtgtaatagc gcgcgaattt
     3301 agtaatgaaa ttttcagctg gtgctttttt actgctggca ttttctacta attctaataa
     3361 tttgctgata gtagaatttt cataagattt ttctacttga actaaaaatg gtttattttt
     3421 attgataaat ccgcttaata ctgtttcacc aacatataca gatttaggga ctgattctcc
     3481 tgtaagtgct gacgtatcga ctaatgattc tccttcgatg atcgttccat ctaacggtac
     3541 acgttcacca ggcttcacaa gtacaaactg accgatctgc acgtcttcag ggttcacggt
     3601 catcaatcct tccgtcgtct gcacgtgagc tgtttctgga cggatcgcca tcaattcgcg
     3661 aatcgatttt cttgaatgac tgactgccaa tccttggaaa tactcgccga tttgataaaa
     3721 tagcatcact gctacggctt ctggatattc accgatcaac atagcgccaa tcgtagcaac
     3781 agacataaga aaattctcat caaagatttt tcctttgacg atattcatca ctgccttctt
     3841 cactacatca tatcctatca gcagataaat caataaatac cctatcaagc gaattggttc
     3901 attaaaatca aataatagca ataaagcgat acctgcaagg gaaaataaaa tccggaacag
     3961 cgtatttctc tctttttcat ttccatgttc agatactttt tccttatcta tttctgttac
     4021 tttgacatct ggttctaatt ccttcactac tttttttgtt tcttgttcaa tcgtagccaa
     4081 atgatcttcc tctgcgtcaa tcgttagttt tccagaagtg aagtttaccg ttgcttcctt
     4141 gactccattg atcttctgaa ctcctttttc gattttcatt gcacagttcg cacaatctaa
     4201 accttctaat ctgtaacttt tcattttaat gacctcctcg cctcaacata tgaacatctc
     4261 ttcataacta attataaatg aatggttatt catatgtcaa ggtatccgaa agaaaaaatg
     4321 aaaaacatct gcatcggaaa atttcttcca tacagatgtt ttggcttatt ctttcttttt
     4381 ctcttcgatc attgcccctg ctcttatcca acccgcactt gccttcttga tcttgacagg
     4441 atagcaacta agtttgaagc ctgttgctgg caatttgtct aaattcatta atttttcgat
     4501 aggacaatat cttttttcaa tccctgcaaa atgtccttcc cagatattcg aagctccact
     4561 acgctttcaa aaatatagaa agagttcttt aatgaaaatt atttcaactt tccataaaca
     4621 gtaatcccat ataaataaaa gacgtaaaag tcacatggga gaacttatac aggaaaacac
     4681 atagagtcag gtgaaataca tgtatcccac ctgactctat gcgttttcta acctttatta
     4741 ttctcttttt atacaatttc tgcaccgaat ggcatcaacg ccaatcgagc aagtttgaaa
     4801 gattgcttgg caaaaggtat ccctatgatc gtgatacata gaaggatgcc gctgacgaca
     4861 tgtgcgagag caattggtag gcctgaaacg atcagccaga tgatattaac gatcaatgac
     4921 atgccagact ggtgataaat tacttctttg ccaaaaggcg caaaagaaag ggaggcgatc
     4981 ttaaaacatt gcataccgat tgggatacct atgatcgtga tacaccaaag tatccccgct
     5041 gctaaccagc taagaccacc gattagtccg ccgaatataa accagatgac atttcctaaa
     5101 caactcattg tatttctcct tctattactg tttttgatta aaactgggca aaaattgatt
     5161 ttattcttct ttttttagct tccagcatca agtattctgc aaattttttt tggatttctt
     5221 ccgtattttc ttttgttact aatgtgtgtt ggattagttt tcctgatggt cgctgatcca
     5281 cccgttcttc tgactgttgc acgtccgctt gcgcaaatcc gataatccgt ttttcttttg
     5341 ttacagcaac gagcaatagt ttattcttat catttaggtc agctgccgtt tttgctttta
     5401 cgaatgattc tggatgatca gtaatcagct gattgatttg aggtaaatct tcttttactg
     5461 cccaccgtat ttccatcatt tctcactttt ctttcgctca tttttatctc cactaaaaaa
     5521 atcgactgcg gcaattgcat ctatccagct ttgtgcaatg atctcatgtc cgccaatcgt
     5581 tgggtggacc ccatcttctc ttgtatattt cgaaaaacca taagcaatcc ctaaagcatt
     5641 gaatatgcca tctaaaggaa ccagcaatgc gccatattcc ttagcaagtt tacgaacaat
     5701 ttggatttta gggtctaagt ctttacgcca gccatgacgg tcgtcggtta taggaagtac
     5761 aaacggctcc atcaagatga tgttgcagat ttccgctgtt tgaagttcat ttagtaattt
     5821 tcgataaacg gattcaaatt gctgcgcaga tttctcagtt ccgaatatag cctgatcgct
     5881 agtattatgc caggtatcat tgattccgat caaaatcgat acatagtcag gttgtaaagc
     5941 taaacaatct tcttgccagc gtacttgcag atctgcaatt ttgtctcctc ctatcccacg
     6001 gttcatgcat tcaaatttca tcgttggata ttgggagatt agttttccgg caatcaaaag
     6061 tggataacct ttacctagat cagttgcttc tgctcggtct cttcctgcgt ctgtaatgct
     6121 gtcaccgata aataatatac gttttttcat ttgtcaaaaa ctcctttttg aataaaacga
     6181 tgattttctt ccttatatta tcccataagc cctatcaaga cgaaagagaa aatatatgta
     6241 taaaaaatga caccagccta gtcccttcaa tcaattgaat gaacgctcat caagtgtcta
     6301 gttttttata ctttttttct taacgtgccg gattcccctt gattccttcg ttgtttggtc
     6361 cagagaaatc gatcacacct aagcttccgt caatcagacc actgatgacg ccatcagaac
     6421 atatatttat aggctcatct aagacttcca gtaaatgttc ttttactaat atctcttcta
     6481 gattgcttgg gatagattgg tcataacttt atctggtccg atgatttcaa ggatgaatta
     6541 aacttacata tggatcattg catgctgaaa atacggacct actccatctt cgaatgttac
     6601 tattagtttg ttttcaggta ctagatgctg ctttatttca acagcagaag catcatcaaa
     6661 tcagatttcc atttgaataa ccttctttgt atatgtttga ttgattgcga tttcgttata
     6721 gaaccttgtg aatcaaatag caatttcttt tgctttcttc tcttgtctgc tggaaacttc
     6781 ctttatcccg tattttaaaa aaagaactgc gagagttcgc agttcttttt tttaaataaa
     6841 cgttgctctc cggccaactc ctcggtcaca agtcacaata gtcatcaaac atgaaaaact
     6901 gttttttgaa attactcctt gtgactcgga gctgaacggc aggcgcacaa cctcttgata
     6961 taaacggtgt tcaatcagct gaccctcgat attagctaaa aaaacggtaa aatatgagaa
     7021 gctatttttc cgttttgttt ctctaatact caggtcaagg cactgatttc acaacctctt
     7081 ccttaaacgg tgttcaaaag ccagtttctc ggtattaagc caaatttatg caaaaatgtg
     7141 gaaagccatt tccacaaaat ttttcttaat actcgaaact aaacagcttt ttcacaacct
     7201 ctccactaaa cggtattcaa aagcttacat ctgcggtaat aagtcccgcc aattcaaaaa
     7261 catgaacagc tgtttttgct tggctgttct aattacttga tgcagaacag ctttttcatc
     7321 acctcttatg gataaattcg gttcaacaaa cgtgggaatg gaatagcttc acgtacatgg
     7381 tcgataccag caatccaagt gactgttcgt tcaaggccta gaccaaaacc agagtgcgga
     7441 acagaaccgt atttacgtag atcaagatac catgaatatt ctttttcatc caaaccgtga
     7501 ttacggattt gttctagcaa gaaatcatag tctgttgccc gttcgcttcc gccaataatt
     7561 tcaccgtaac cttctggtgc gatcatatct gcacaaatta ccacgtcttc tcttgttgga
     7621 tgtggtttca tgtagaatgg cttgatggct tttggatagt ttaggataaa gacaggctgt
     7681 tcgaatgaat tcgcaatgaa cgtttcatga ggtgaaccaa agtcttcgcc ccattcgatg
     7741 tcatcaaatc catttttctt cagtaattcg attgcatcat cgtaagtaat acgagggaat
     7801 ggcaattttg tataagtttt taagacttct ttgtctcgtc ctagtacatc taatgcatat
     7861 tcgcaatgat ctaaaacatt ttgtactaag aatgctacat attgttcctg tacttctaaa
     7921 ctttcttctt gatgagtgaa agccatttct ggttcgatca tccagaattc gatcaaatga
     7981 cgtcgtgttt ttgatttttc agcacggaaa gtcggaccga atgaaaagac ttttccgaaa
     8041 gccatagcag cagcttccat gtataattgg ccactttgag ataaataagc tttttgatca
     8101 aagtaatttg tttcgaatag atccgtagtg ccttctggtg cagaaccagt caagatcggc
     8161 ggatcgattt tgacaaaatg atgttgatta aagaattcat acgttgcacg aatgatttca
     8221 tttctgatct gcataatcgc atgttgtctt gaagaacgca gccataaatg acgatgatcc
     8281 atcaagaaat ctgttccgtg ttcttttggt gtgatcggat aatcgtggct ctcgcttacc
     8341 acttccacat ttgtaattcc gatttcataa ccaaattttg aacgactgtc ttcacggatt
     8401 tctccagtta ctaacacgga tgtttcttgg tgtagatttt tagctgtttg gaaaacttct
     8461 tccgacactt cgctttttac aactacacct tggaaaaagg ctgttccgtc tcttaattgt
     8521 aaaaatgcga tttttccgct tgatcgctta tttgcgaccc aagcgccgat tgttactgtt
     8581 tctcccacat ggtttttcga atcgatgatt tgaatttgtt ccacgtatga ctctcctttt
     8641 atattccttg aatttcttca ggattaattt tgacttttac tctcaataaa ttgcttgatt
     8701 ctgcgtaccg cttcttctaa cgtagcgcga tcagtggcat aactcatgcg gacattttct
     8761 ggtgccccaa agccttcgcc agtcacaagc gctacccctg tttcttctaa aagatcttct
     8821 acccattgtg tgacattttc atacttacac atgcgtagcg tttcttttac attaggaaaa
     8881 agatagaaag ctccttgcgg cttttcaaga gaaacacccg gcagttctgc cacaagcgga
     8941 tataacgtat tcaggcgttc ttcaaaggcg ttccgcattt cttctactgt atcttgctca
     9001 cctttcaatg cttcaacagc tgcatattga ctgactgctg tcggattgct agttgattgg
     9061 gaagcaacag cgatcattcc attgatgatc acaggatttc ccaccgcata accaatgcgc
     9121 cagcctgtca tcgcatatgt tttagacacg ccgttgatga tgatggtctg atttttgatt
     9181 gcttctgaaa tcgttgcaat tggagtaaac tcattgccat tataaactaa acggccataa
     9241 atatcatcag caactatcaa aatatcattt tttactgccc attcaccgat tgcctgcagt
     9301 tcattcttgc tataaatcat tccagtcgga ttcgatggag aattgatgat tacggctttt
     9361 gttttttctg aacgtgcttg ttctagctgt tcaacagtaa ctttccagct attggattct
     9421 tcccctttca caaagacagg aagtccttct gccagtttta cttgctcacc gtaactgacc
     9481 cagtaaggca caggaatgat gacttcatct tgcgggtcaa ggatcgtttg gaaaagggta
     9541 tacaaagcaa atttcgctcc gtctgttacg actgtttgtg aaggttcata gacaagtcca
     9601 tagtttttct tcagataatc aacgattgcc tgtcgaagct ctgggattcc tgctgttggt
     9661 gtataatagc tagctttccc attgcggatt gcttcgattg ctgcttcttg gatgttttct
     9721 ggtgtcgcaa aatccggttc tcccacagtc aaactcagca catctctgcc ttttgctttt
     9781 aatgccttgg cctttgccgc agctgccaaa gtgacagacg gttctaatcg ttttgttctt
     9841 tttgataact ccatcccatt cattccttta ccttttaaat tcccttgatt gtcttgactt
     9901 catctccaga ttcaaagcca atcaggtaat aattcagttt cccatttgtt tcttttccta
     9961 ctacttccca cacaggctga tcatcataca tccctaaagc agctttttcg atttggattt
    10021 caggatgggc cgcagcaatt gtctgttttg cttcgttttc agtcagtccc tttgtttgat
    10081 ccaatacttt tactttttca ccagatttag gaatgataac ggcgatttct tttcctttat
    10141 catttgtacc tgtcagtgaa aagtatgtct tctttcgagt aaaccaataa aaatgatcgg
    10201 tttcctcaag atttgcatat ttctttgcga tttctactgc ttctttcttc gcctgcaccg
    10261 ccggacgact ggaacgcaag taaaaaatcg agctgatcac gatgaccgct aataaaaata
    10321 cgatcaagcc aagtaacacg cgattaatcg ttttttcctt tgtctgtttt ttcttcaagg
    10381 gtcgtcgctc ctttatcttc attccttttc attatagcag attttaatta tgaaaaaaga
    10441 cagctcctct aacttccttc ttcatttttc tttaagaatt catggatatc ctgatagatt
    10501 tcctccatag ctagctcttt gatcggtact tttttcggta atgcatggat gatccgtgcg
    10561 ccatattttg cagtgactag tcttcgatcc aaaataagca atacgccacg atctgtctcc
    10621 gtccttatca agcgacctaa tgcttggcgt aacttcaaag ctgctttagg tacggattcc
    10681 tggtaaaaag gattgatccc ctctgattct aaataagcgt tcctagcttt gaccatcggg
    10741 cgaagcggat tatcgaaggg taaacgtgtg actacaacga tctgcaaagc atctccaggc
    10801 aaatctacac cttcccaaaa gctgtcagcg ccaaacaata cgcttttttc tgacaaaatg
    10861 aagcgtttca aaagcttctc ccggcttcca ccaaaccctt gagctagtat ttctcgtcct
    10921 ttttctagca tagacagatg cattttctga tagaccttct gcaaaacatc atgagaagtg
    10981 aataacacaa gtattggccg atcttcttgt tcgatcagac ctgtaagtgc tgtctctaaa
    11041 taatcgatat atgcttgatt gctgacttga tggacggcag gtgcctcttt tggcacatag
    11101 atccttgcct gtttctcata atcataagga gaggcaatca ctttaagagg tgctgcttca
    11161 atgccaagcc tcttagagag atagtgtcga ttagggccga tcttcagcgt tcctccaata
    11221 taaagaatac gatcatatcg aggataccaa acagtcgacg gcaacatagc cgcttgaaaa
    11281 tcatggagat gaaaaatcat ctgctgcgct gtgttcccag gaacgaacca atggatcaaa
    11341 tgatctcgcc agtcttccag ccattgatcc atcagcaagt attgcttgtc taattgatca
    11401 aataggctat ataaacttgc taaatcgatc cgcatctggt ttgtccagct ttcttggata
    11461 atctcaaaag tcagacgcat ttgtgactgt agctgctgga tctcctgata aaaaagctgc
    11521 aggtgatcaa tggctgtccc agattccagt ggcagctgat ccaacatctc ttttgtcatc
    11581 atgacttctt ctggatactg tccgttaggt tgcttgccgc tcactaaact cattaatcct
    11641 tctgccatac aaatctgtgc ttcgcatagc gcagaaagtt cttctcgata aatcgataac
    11701 cagcgtaagg cttcatgttc tttcggtaaa tagctttgta ttcggtcaaa cagctgattt
    11761 ggttcttcta attgctggat ttgtttatgg aaatagttaa tacttaatcg tttgtcattt
    11821 actttttcag cgatttccgg caaatgatgt acttcatcga tcaacaaaaa tggactagct
    11881 ggaagcaatg gttcttttcg ctgtgtttct tgtactaaaa acgcatgatt gacaatcaaa
    11941 aaattacttt gcctgatttt cttttgcaag aaacgaacaa agtcttgttc gtaaaatgac
    12001 tgatttcttg ctaaaaatcc aatacctctg tgcgcgatat ctgcaaagaa tgaatggttc
    12061 aaatttgtta aatgtaattc atccaaatca ccagtcttcg tttgcgtcag ccaaactaag
    12121 accgccattt gatacaaagc atattgtttt tgttctggcg gttccacaag tgtgccttta
    12181 aaacgctgca aatcaatata gtggcgatag cttttgataa ttacaggttg tatgggctga
    12241 tctagcaatt gattcagcaa tggaatgtct ttttctatga tctgctgctg caaaactagc
    12301 gaaacggtac tgatgacagc gggtttttca ggtgtagcca aataactcaa tggcagcaga
    12361 taaccgattg ttttacccat tcctgtagct gcttcgatca tcaaatcttt tgattcatct
    12421 tttgtgaaat ggtcgtagac cgcattcatc agtctgttct gttcttttcg atacatcagt
    12481 gttttaccaa acattttttc ttttgcttgt ttttttcttg gatatgtccg ctcgccataa
    12541 tggacggatg tgaatagttc cacttctttt ttctgcaaag ctagcccatc gacgatttct
    12601 aatgagggat caagtggctc tggtttttct ttcatttctt caatcacatg ataaatgaaa
    12661 cgactcgtat ccatacctgt gtgtctactt aatcgcgcaa tctgctctaa cgtgatgatt
    12721 ggcaattttc tcatccgttc ttcgatcagc aaaagaagtt gacctgtcac ttcggcatca
    12781 ctatctgcct gatgaggatt ctcatgaata aatccaaggc tttctgacaa atcagctaag
    12841 cgaaagcttg gttccgtcgg caagaaaatt tgtgcaagtt ctactgtatc gatgcctggt
    12901 atcttcaaac taggaagtcc gcatcgcatg agttcatggt tcaaaaaatt ataatcaaaa
    12961 taaatattat gcgcgacaaa tatggtgtct gccaaaagat tgtaaatcgt ttgggcgaca
    13021 tcttcgaaat aaggcgcttt ttggacgcgt tgattcgtga tccctgttaa atgctggatt
    13081 tgttttgaaa tcttccgccc tggatgaata tcgatcgaaa aacgtgaagt gatccgacct
    13141 gattcaatca tgacgcagcc aaattggatg atccgatctt cttttggatt cgtcccagtc
    13201 gtttcgatat caacgactgc ataagttctt ggtttcaaaa tatcttcctc ttctcctgcc
    13261 tctaatgttc ttttgaaatt atactacaaa gtgcagaaaa atactctcat cgctttttaa
    13321 agaaaataga caaaaacgcc cagacacgca aatgtctggg cgtttatttt ttagtatagg
    13381 ttaattaagc tttagtaacg tttgttgctt gaggtccgcg ttggccttct tcaacatcga
    13441 aagttactgc ttggccttct tctaaagttt tgaatccgtc gccttggata gctgagaaat
    13501 gaacgaatac atcgtttccg ttttctgcag tgatgaatcc aaatcctttt tctgagttaa
    13561 accattttac tgtacctgtt tccataataa aaatcctcct tgtgtatcaa cacatatttt
    13621 gcaattactt gaatggtgta tatctggaag atgtttattt gaaacaagct accacagatt
    13681 accgaacaaa aactactcct atagtatatc ataccgttta caaaaaagct agagtttttc
    13741 gacttttttt attaaaaaaa gttttctcag cgattacata aagtgaaaaa ccgaactttt
    13801 ttttaaaata aacgacatat tgttcagttg gtgttttttt gctctgatgt tcttttttaa
    13861 actaaaaaaa ttattctttc tgactactta tcggttctgt ttcaacggtc gaggctgggg
    13921 aagattcttt agtaggtctg atgttctcat ggtatttctg atagcctatg acagttacca
    13981 gaagaacaag gcaaaccaat acgaccaacg agattagttt ttcttttttg ctcatatcta
    14041 tacctcataa ataacaggat gatgtccatc atatttctga gtatgagtaa aagcaaaagc
    14101 tgcagctgta gaatgtaaga aatgccagca actttcataa tcacaggtcg gacagtacca
    14161 aaagtaacca aatcttggct gcaaagaccg ttcttctgtt tcaggaatcg cagaatcaag
    14221 atctggtgtc tgtacataaa catttttgta cagacgtgtg cctgtaatag gaaaccgctg
    14281 tgtttcttca gcaaaaactg gctgacttgt taaacttagg gatagagaca gcaaacacca
    14341 taacagtctt tttttcataa agacctcctt atcgataagt ttttcccaat taaaatcata
    14401 tctcaagaac gcccgttatt cacattttgt tcataaatga tgttttttgt atcataatcg
    14461 tcaaaaagag gtcgtgacca cgtcacaacc tcttttttaa acggtgttcc agaagtagct
    14521 tcttcggaaa taagccgaaa tttcacaaaa atttgagaaa taatttttgt caatctcttc
    14581 ttatttcttg aagctgacca cttctgtcac aacctcttcc ttaaacggtg ttcaaaagct
    14641 tgcatctgcg gtaataagcc caccaaacaa aaaatatgaa gagctatttt tgcttgctgt
    14701 tcttattact tgatgcagaa cagctttttc acaacctctc atacacggtg tttaatcagc
    14761 tgaccttcgg tattagctca aaaaacggca aaatatgtga aactattttt ccgtttgttt
    14821 ctctaatact caggtcaaaa cactgatttc acaacctctt ttaataacga agaatcgctg
    14881 tcagactctt ttcatctggc gcatcttttt gatccaaaat aaacactttt ccgccattaa
    14941 tgatcacatc atctgctaat gcattcaaca cttgacgccg atcatattct gtttcaggat
    15001 cttcaccgaa accatcagct aagtttgacg tagcgataaa taaatgtgac acttttcctt
    15061 ctttcgcagc tggaacgata tcaactaact gatcgatgaa tttcttatct aagagtttat
    15121 tgtattcttt tgattcttga gctgcgagtt cacttgacag cttcttcgcc ccttcttcga
    15181 tatctctaaa ggatagctgc gcaggagaag cagaaatcgc aacgtctgca caataatagg
    15241 gatttttagc aatttttttg aacatcgtct gattttctgg tagagcaaat aaataaatcg
    15301 gccattgttc tggattttcg aaagaatcct tcaagtacgt atcaactgct tggtaatagt
    15361 tgacccaatc gatctctact tcttcatctt ttggatttac gccatggaaa gttactccat
    15421 ctcgggctcc gcctcctcca ttagaagaga agttcaaacc gccacctgtc agttcagatc
    15481 ccaatgcagt aacgacatca accggtgccc cttctggtag agatacttct tttacttgtt
    15541 tgttgctgac ttcatacaat ttcatggagt cgcggttcaa tcccaataaa taataagaat
    15601 agttgaactg cccatttttg ataatagcca gtaaataagg aagatcactt acatagtatt
    15661 gatcgtctac cgggatactc aaacgatgga tataggtttg gtcagcagtt ataatgaccg
    15721 ctacactcgt agttgcacta cgccaaaacg aaggatctcc taacaaattt tcaatttttt
    15781 cttggaacgg ggaccagtca tgttcggaat attttttctc aaaacgtttc tttgcttctt
    15841 tcgcaaaatt tttgaatttg atctgatctt tttccacatc ttgatgggcg acatgcgtat
    15901 tcagcatgat cgtgacgaat ggtcctttag cgccttctga agtcaattgt gacaataatt
    15961 ctttatcctt ttttgccata aacgtacccc tcctaaaaaa atcattcctt tgtttctatc
    16021 taaagtctac cacaacacta tttttccata caataaaacg tatctaaaaa aataaaacgg
    16081 tttctacggt tttaaattta gctatttttt taagttatct tcataatttc ctcaattatt
    16141 tagccccagt cttcaactat tcttcatgaa atgctgataa actaagaaaa agaataaaag
    16201 aggtggatct tatgcatttg attatgattt tcagtttatt aagtattatc agtttatttg
    16261 ctttcagcgc ttcccgctta ttagatgcgg aaacacagcc tattcctgta aaagtacgta
    16321 aaaacgaccg ctaaccttcc ataacgtgcg ttcttatcct aaagtttttt cagaaacatt
    16381 gaaactctct cccctctctg ataaaatttt aatttgtagc gaaacaaatt aattacaagg
    16441 agatcggata tgtccataaa aaaaacacgt ctagtcctat tattggctag tctcattctt
    16501 tgccttgctt cttttctacc agctgttgct gtgcaagctg aagatacttt caaagtaaat
    16561 gcaaaagcag cctttgctgt agatgcgcaa agcggtaaga ttctttatga tcaagatgga
    16621 gaaaaaacca tggggatcgc ttcaatcaca aaaatcatcg gtctttacat cgtccttgat
    16681 caagtaaaag aagggaaact atcatgggat gataaagttt caatttcaga ttatgcagaa
    16741 aatttgagtg tcaccccaga tttatcaaat gttcctttgc ataaagaaaa cacctacacc
    16801 gttaaagaat tattcgactc tgcaatcatc cagtcagcca acgcttcaat ggttgccttg
    16861 gcagaaaaaa tctctggcag cgaagcaaaa ttcactgaac gaatgaaaga acaattgaaa
    16921 gattggggta tcaaagatgc aacgatcgtt aatgcatcag gactgaataa ctcttatctt
    16981 ggcgaaaacc gaccagaagg tactggagaa aacgacgaaa atcaaatgtc tgctcaagat
    17041 gtggctatcg ttgcccgtca tttgatcttg gattttccag aaatactgga tgtatctagc
    17101 actacgacac aaatgtttgg cgaaaacacg caatcaccag tagaaatggt caactggaac
    17161 tggatgctcc caggattcgt gaattataaa gaaggcgtgg acggattaaa aactggtacg
    17221 actgaacttg caggagcctg tttcgtcggt acgatcacta aaaacgatca acgaatcatc
    17281 actgtcgtat tgaatgcaga cggccatgcg gaaaacccaa gtgcccgttt caatgaaaca
    17341 gctcgcttaa tggattattg ctacgacaat tggtcagaaa aagagcttgg aaaagccaat
    17401 gcaagtatcc ctagtctaaa aaccattgat gtaaaagacg gaaaagaatc aacagtgccc
    17461 gttgtgctta aaagtcctgt aaaagtatgg gtacggaacg atatggatac tggaaaatta
    17521 acaatcacac caactcttga caaaaaacaa gtcactgaca atgagatcca agcacctatg
    17581 gataaaggaa caaaaatcgg taccgcaacg atcaccttgg cagaagataa attaggttat
    17641 cttgaagaca acaacgcgcc atccacagaa atcatcacgg ataaaaccgt agaaaaagca
    17701 aatattttcg ttattggctg gcgacgcgtc gcagaatttt tcggtaactt gttttaattg
    17761 aacaaaaaga aaaaagcatc tttccatgtg gaagatgttt ttttgctttt tctagtttaa
    17821 ataacagctt ttttcatcta ttttcgttat actgtataag aaaaaggagt tgaatttatg
    17881 aatctaacca gagatctgat ttttttggtt ctctatctta ttggggtatt ctccatcgtt
    17941 tattggcgaa aacagaaaaa ggatccttcc t
//
LOCUS       DANKKG010000050        17889 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000050 DANKKG010000000
VERSION     DANKKG010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.050"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..757
                     /locus_tag="PW004_001411"
     CDS             <1..757
                     /locus_tag="PW004_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726393.1"
                     /translation="VHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSASAGEYE
                     SHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISELLYFGHM
                     KTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKINVKKSF
                     FKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIWKLKGTR
                     YREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(838..1407)
                     /gene="thiT"
                     /locus_tag="PW004_001412"
     CDS             complement(838..1407)
                     /gene="thiT"
                     /locus_tag="PW004_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HDL2726394.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(1473..1575)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(1581..2552)
                     /locus_tag="PW004_001413"
     CDS             complement(1581..2552)
                     /locus_tag="PW004_001413"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HDL2726395.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(2646..4019)
                     /gene="glmU"
                     /locus_tag="PW004_001414"
     CDS             complement(2646..4019)
                     /gene="glmU"
                     /locus_tag="PW004_001414"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HDL2726396.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(4522..5340)
                     /gene="purR"
                     /locus_tag="PW004_001415"
     CDS             complement(4522..5340)
                     /gene="purR"
                     /locus_tag="PW004_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HDL2726397.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(5507..6331)
                     /locus_tag="PW004_001416"
     CDS             complement(5507..6331)
                     /locus_tag="PW004_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDL2726398.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(6304..6984)
                     /locus_tag="PW004_001417"
     CDS             complement(6304..6984)
                     /locus_tag="PW004_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDL2726399.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(7007..7945)
                     /locus_tag="PW004_001418"
     CDS             complement(7007..7945)
                     /locus_tag="PW004_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HDL2726400.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(8190..9053)
                     /locus_tag="PW004_001419"
     CDS             complement(8190..9053)
                     /locus_tag="PW004_001419"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HDL2726401.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(9195..11768)
                     /gene="mprF"
                     /locus_tag="PW004_001420"
     CDS             complement(9195..11768)
                     /gene="mprF"
                     /locus_tag="PW004_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HDL2726402.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            11909..12715
                     /gene="proC"
                     /locus_tag="PW004_001421"
     CDS             11909..12715
                     /gene="proC"
                     /locus_tag="PW004_001421"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDL2726403.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(12794..14350)
                     /gene="rny"
                     /locus_tag="PW004_001422"
     CDS             complement(12794..14350)
                     /gene="rny"
                     /locus_tag="PW004_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HDL2726404.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(14761..15810)
                     /gene="recA"
                     /locus_tag="PW004_001423"
     CDS             complement(14761..15810)
                     /gene="recA"
                     /locus_tag="PW004_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HDL2726405.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(15919..17160)
                     /locus_tag="PW004_001424"
     CDS             complement(15919..17160)
                     /locus_tag="PW004_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HDL2726406.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            17384..>17889
                     /locus_tag="PW004_001425"
     CDS             17384..>17889
                     /locus_tag="PW004_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2726407.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSM"
BASE COUNT         5147 a         3896 c         3129 g         5717 t
ORIGIN      
        1 tgtacatatt gataccacaa gtaatgctgt tttaacaaaa gggctgactg ctactgactt
       61 tgcaaacagt atcgtgcatt ttcctcaaaa cttgctcttg cttgatccat ctgcttctgc
      121 tggagaatat gaatcccata cagggctgaa agtaatccgt ggtacagaaa atattcagcg
      181 tttcttttcc tcttctcgta acaggcgtgg attcgatgaa ttgaagtgga tcgactttac
      241 tgatttaacg atgctgaaag aattgacacc tttagaaatt tcagaattat tatattttgg
      301 tcatatgaaa acacatcttc attcgccgtt tttttataaa ctacagaata attttgtata
      361 cttcgatctt aatgatcagc tgaatcggat ttattatcgc tatttagatg aattttatag
      421 gatttttgct agtaaattaa gttcgatctt gtcagataag atcaatgtaa aaaaatcttt
      481 tttcaaaaaa gaaacacctg ttgaaaaaat cagtgtggaa ttattgaaag aaatgcgtaa
      541 tttgatgcag gaagggatca ttttttcttt tgatcaagtt ggtttgatcg atggtaaata
      601 tcgtatccca atctatgtga ttgaagatta tatttggaaa ttaaaaggca cccggtatcg
      661 agaagaggaa atcatcgcta ctttaatcta ctctgtcacc gatcgacact ggcaattggt
      721 agaagatgcc gatctttctt atctgccatt gaagtagatc tatttcttat caaaaaataa
      781 aaaggaaaca agaaactagg acatgattct gtctcaagtt tcttgcttct tttttattta
      841 cagctcctgt cgtttatctg gcacaaaaat cgaaggagtc gtttgttgga tccctaataa
      901 aacgatgatc gttacaatag ctgtactcaa cccgctggca ccattcatta cgaaagaaaa
      961 gagccaagga ttcatccccc atagtgcata cgctccccag aaaaccactc ctgcaataaa
     1021 atgccaaaaa tatctggaaa gtgcgccaat caatgcacca aacacgatgc tttttctaat
     1081 ttgtttcgta ttatcgtcta catagccttt ttggacattt gaagccatga gccctgctgc
     1141 gcctgcaaaa gtataggcaa tcgggtactc aatcaacact tgaacgacac ttaaataata
     1201 aacttgtccc aacggaaaat gtaggatgcc ccaaatgaaa cctgccagca tccctgcctt
     1261 tgtccctctt cttaacgcaa aaagagtcaa tggaatctgc cctaatgaaa tagaaaaact
     1321 gctgccgatc tggatcggaa tcaacgacaa caccatcgct aacgctgcaa caatcgttcc
     1381 ttctacccaa actctatttt ttcccataaa aaaacttcct cctttgaata aaaaagtcac
     1441 aaaggaggaa gcagctttcg aaaatagtca aacagctgtt tattcacaat tcctacgctc
     1501 gcataaccga tacaggtacg aagggtttag aaatagattc aatctcagcc caattggact
     1561 cccctttgtg agtttattta ttatttattt tttgtttcaa acaatggact aactggtttg
     1621 ttttcatgga tacgtttgat tgcatctccc attagtccgc caacacttac ttcatcgatt
     1681 ttgtcgattt tacgatcttc tggaagatag atcgagtctg taacaactag acgctcgata
     1741 gctgaatctt ctattctttg taaagcaggg ccggataata ctggatgtgt acatgaagca
     1801 tacacactag ttgcaccagc ttctttcaaa gcattggcag ccaatgtgat cgtacctgct
     1861 gtatcgatca tatcatcgat cagtacacaa gttttgcctt tgacttgtcc aatgatattc
     1921 attacttctg caacatttgc ttttggacgg cgtttgtcta tgatcgcaat cggagatttc
     1981 aaaaattccg ctaatttacg tgcccgtgtg acaccaccat ggtctggtga aacaacgaca
     2041 acatcatcgc ctttgattcc tttttctaag aaataattgg caataagtgg cgcacccatc
     2101 aaatgatcga ctggaatatc aaagaaacct tggatttgaa ctgcatgaag gtccaaagtc
     2161 agcattcttg ttgcaccagc tttttgaatc atgtttgcta ctaatttcgc tgtgattggt
     2221 tcccgtgcac gtgctttacg gtcttgacgc gcatagccat aataaggcat gactacgttg
     2281 attgtttttg cacttgctcg tttcagtgcg tcaatcatga tcagcaactc catcaagtta
     2341 tcattgactg ggctgctcgt tgattgaata acatagacat gcgatccacg aatactttct
     2401 tcaatgttta cttgaatttc tccatcacta aattgagtaa cagatgattt tcctaactcg
     2461 acgccgactg ctgctgcaat tttctctgct aatgggcgat tggaatttaa cgcaaaaatt
     2521 tttaatcttg ggtcaaagta atgttttgac atggggacct ccactttctt tttcacaact
     2581 ctagaattct acctaataaa tagtagtcat attttttaaa taaatcaagt gattttgcaa
     2641 aagtcttagt ttagataagg gagttttttt gcatatcctt ctttattgac ttgtcttgct
     2701 ctcgcaattg ccagcgcata ttctggaata ttgtctgtaa ttgttgaccc ggcagcaatc
     2761 gaactgtttt tagctacctc gacaggtccg atgatattcg tgctagaacc aataaagctg
     2821 tggtcgccga cagtcgtatg atgtttgttc tttccatcat agttgacgaa gacaacacca
     2881 catccgacat tgatttcttc tcccagcgta gcatcaccaa cataagtcaa gtgaccgact
     2941 ttagttcctt tgccgatttg agcatttttc acttccacaa agtttccgat atggacgttt
     3001 tcgccaattt cagcttttgg tcgtaaatgt gcataaggtc ccacatctgc atgtttcttg
     3061 acatggctgc tttcaatgac tgagttttca atgacgacgt gatcctcaat cctactgtct
     3121 acgatttttg aatgtgaccc gatgacacaa tcagaaccaa tgactgtatt tccttggatt
     3181 tgtacacctg cttcgatcaa tgtgtctgga ccgatttcta cgcctgcatc gatgtatgtc
     3241 gttgctgaat cgacaaagct tacgccattg accatatgca tctggttaat tcgctttctc
     3301 atgatctcat ttgctttagc taacgcgatc cgatcattga ctcccattga ttcttcaaaa
     3361 tcatccgttt gataagcagc aactgtatgg ccttcttctt ttaagatttc gatgatatct
     3421 gttagatagt attcaccttg agcattgttt gttccaactt tgcttaacgc atcgaacaaa
     3481 gcttcgttgt caaaacagta agttcctgta ttgatttctt gtacaagagc ttcttctggt
     3541 gttgcatctt tttgttccac gattttttct acgatcccaa tatggtcacg gatgattcgt
     3601 ccatatcctg ttgggttttc tgcttgtgcc gtcaagatcg ttgcacttgc attttttcct
     3661 tggtgatatt caaaaagatt gttcagcgtt tcagtagtca ataatggtgt atcgccactg
     3721 ataactaacg ttgttccttt ttttccttcc aagaaagaag cagcttgcaa aacagcatgt
     3781 cctgttccca attgttctgc ttgtaacgca tatttactgc gttctcctag ttgtgcttta
     3841 acttgttctg ctccgtgtcc aacgatcgta atcacttcat ctggtttcgt ttcactgacc
     3901 cgattaatga tatgttctac cataggttta ccagaaacgg gatgtaaaac tttatataat
     3961 tttgacttca tgcgggtacc tttccctgcc gccaaaataa ttgcataacg tgcgtccaac
     4021 tgcgtcactc ctaaatatag tttgctgatt tcagtttatc ccagtatacc cattttttca
     4081 acaaaacaag tgattttctt ttatcccagc gttttctgga taaaaaaata gatagaccat
     4141 aacgggccta tctatttggt aaccggtgct ctcctgccaa ctcctcggtc acaagtcaca
     4201 ctactcataa aacatgaaaa gctgtttttt gaaatcgttt cttgtgactt agagttaaac
     4261 ggtaggctta caacctcttc attaaacgtt tttcaatcag ctgaccttcg gtattagctc
     4321 aataaacggc aaaatatgag gagcaatttt tccgtttatt gagctaatac tcaggacagg
     4381 acgctgattt cacaacctct agataaacgg tgttcaaaag ccagtttctc ggtattaagc
     4441 caaatttatg aaaaaatgtg gaaagccatt ttcacaaaat ttttcttact actcgaaact
     4501 aagcagcttt ttcacaacct cttattctga aaaataattt cctggcacaa ccgtaatatt
     4561 tttcgtttga gtatctacat ctttgacata aagcagtgac gtataatcat cgatggcgcg
     4621 atgaccgttg aattttgatt cggcaaatac agtgatgcca acaagttctg attcgaattc
     4681 ttcaatcaag ctcttcattc cgttgactgt tccgccgcct ttcatgaaat catccactac
     4741 aaggacacgt gaaccacgtt tcaaacttct cttagacagt tccatctttt caattctttc
     4801 agaagatcca gacacatagt tgacacttac tgttgatcct tctgttattt ttgagtcccg
     4861 gcgtacaata acgaatggcg cattcagata ataggacaca gcttgggcaa taggtacgcc
     4921 ttttgtggct acagtcatta cagcatcgat tttttcacca aggtatttag aagcaatgat
     4981 tcttccaact tgacgcagca gttcaggttc tcctaacaaa tcagagagat aaacatatcc
     5041 tcctggaagc agacggtctt gttctgatag acgttctgct agagattcaa tatatttttt
     5101 cgcctcttca tatgaaattt cagggatgaa caatacacca cctgcagcac ccgggattgt
     5161 ttccaaggtt ccataccctc gttctttaaa tgttttcttg atgattccca aatcttcact
     5221 gatagaagat ttagcagatt cataacgttc ggcaaagctt gttaaggaaa tcaattcatg
     5281 cgggtgattc aataaatact gtgtcatgtc aactagacgt tcactgcgac gtactttcac
     5341 ctattccaac tccttttaag aaattttatc gttttacatt atataagttt ttcttcaaaa
     5401 attcgagcat tttatgaaaa aataagaaaa tagttcggta tttattaaaa taaaaagaag
     5461 cctataaaaa atttttcgtt tttataaggc ttcttttctt tataatttag ttattttttc
     5521 ttctgaaggc aaccaatatt cgtttgatga tatttacaat caggaacaaa gcaataaaga
     5581 ctaatgtgat tgttgctcct ggaggtgtat ctagataaaa cgagctagtt agtcctgaca
     5641 gcatccctag aaatcctaca caaacactga tagcaatcac gacattgaag cttttgccta
     5701 atcgcatacc gattgctgcg ggaagaacca tgattgcgga tatcaaaagt gcaccggcaa
     5761 tcgggatcat cacagcaata gccacaccag taatcacatt gaacatcatc gacatcaaat
     5821 gaacgggtag cccatccacg tgggcggtat cttcatcaaa agtcagaaca tacatcggcc
     5881 gtttgaataa aaagaacaat ataccgatgg caacgaataa gattcctaaa aagatgactt
     5941 gctcccatgt aatggtcacg atcgaaccaa acagatagga ttggatactt gttgctgaat
     6001 tccctccgct caaattcatc aatactaacg ctagcgccaa accaccagcc attaaaatgg
     6061 caatggagat ttctgaatag gttcggtaga tcgttcgcag atactccaat atcacagctg
     6121 ctaatacaac gactagcatg gtcgtaagtg taggattaag gttcaaaaag aatcctaacg
     6181 ctacccctgc taaagagaca tgagaaagcg tatcagccat cagcgattgt cttctgatca
     6241 ctaaaaacac acctagcatc ggggcgattc cggcaatgaa gatagcggca agaaacgctc
     6301 ttctcatgaa ttcatatgaa agcaacgcca ttgagaatcc tcctttctca ccagtcggat
     6361 ctgtctgtcc gcatactgtt tgatatcttc atgatcatgg gtgatcatta gaactgattt
     6421 cccatggaca tgcgcactat gtcggagaag acgataaaat tcatttctgg attcttcatc
     6481 catacctgtt gttggttcat caagaacaaa taagtcaggg tcagtagcaa aaatacgcgc
     6541 taaactgatt cgctgctttt gtccgccaga caattcacct attcgccgat ttcgcatatc
     6601 ccacatccct acagcttcta aagcacgctg tacatgttga tgatcttttt cagagagacg
     6661 cttgaaccag cggcctcttg gatatcttcc tgaacggact aattcgatca ccgtactagg
     6721 aaaacctgca ttgaaagatg caacttgttg aggaatataa ccgatactta atttttttcc
     6781 ttcttgattt ttctcagcaa tctttatcat tccttttgaa ggtttcagta atcctaacga
     6841 tgctttcacc aaggttgact tggctgctcc attttctcca gttaaaatga caaattcacc
     6901 cgcatctaca tgataagaga catcttctag tacaggttca tcatcatagt aaaaggtcaa
     6961 atcagatatt tcgatataac gcaccttaca acctccattc taaaacttaa ttgatacttt
     7021 cttttaaagc tttcagattt ttttccatga cagaaatata attttcgcct gctttgattt
     7081 gttcattagt caagctttct aaaggattca acacttctag cttcactcct gtttcattcg
     7141 acaatgtctc agctaccttc gaagaggcat tttcttcaaa ataaatcact ttgatgttgt
     7201 ttttcttcac aaattctttt agctcagcta agcgacttgg tgtgggttcc tgatctggtg
     7261 aaacgcccga aatcgctact tgttttaagt cgtattgatt cgctaaataa gcaaaagcag
     7321 catgctgagt gacaaatgtg cggtttgttg catcttttag ctcatcggag tatttttgat
     7381 ccagtgcttc aagctttttg atataagcat cggcattctt cgtaaaagtt tcctttttat
     7441 ctggatattt ttcgcctaat tcttcagcaa tcgttctgac ttcttttata gctaataccg
     7501 gatcgagcca aacatgtgga tcgtattcat gtgaatgacc atgcccttct tctgattctt
     7561 catgttcgtc tgcttcttgc gattctaaac ggtcgattcc tttagctgct tcgatcatca
     7621 aggtctgttt gctatccact gaatccttta acgaatccac aaaaaattcc atgtctgaac
     7681 tattatacac gaaaacatct gcatcggaaa tttctgctct ttcttttgca cttggttcga
     7741 aatcatgcgg ctccgttcca gcagggatca aaagtttcac gtttccttca tcccctacga
     7801 tctcttttgt aaaatcatac atcggataaa aagtcgtaac gatcgtcaaa tcctcttttt
     7861 tgtctgcttc cttgttcgta tttccacatg ctgcaaacag taaagcacct atcattaccg
     7921 tagctgtcaa aagatatttt ttcatttata tgctcctttc aatgaacaaa tcggaatttt
     7981 acgatttatt aacgtgaact aatgtatcaa aataaaaaaa cacagtcaat agaaatccat
     8041 caatttctga tttaagcatg tatattcttg attaaatcgt aaaatggatg agttgctgct
     8101 catatctagc catttcacta caataaaaaa ctgaagaata ccattttagg attcctcagt
     8161 ttttcatcac ctattttact tcttatattt tattgcaggg ttcgtactaa ataaacttcc
     8221 tcacaaaaac cttttaaccc attatatact ctctgcgctc ttgaataatg ttgacacaag
     8281 gcaaaaacgg ttggtccgct tccactcatc aatgcagcat ctgctccgta cttgatcata
     8341 cgctctttga tttgatggat gactggatgc cgctttcccg ttatatcttc taaactgttt
     8401 cccatttttt tgatcatctg ctgataatct tgctctcgga tggcttcagc aagtccgtgg
     8461 atgtcttgat gttggatggc ttgcgtatct acttctctaa acactgtacg tgtagagaca
     8521 ctcattcttg gtttgaccaa cacaacccag cactgtggca tagaaggtaa tacttggatt
     8581 tcttcccctt tgccaccgac aaatgccgta cttccgtaga cacagtaagg aacatctgta
     8641 ccgacttcca aaccgatttc tgccagttct tctaacgaac agcccagatt ccataattgg
     8701 ttgatccctc ttagagcagc tgcacagtct gtacttcctc cgcctaatcc agctgctact
     8761 ggaatattct tcttaacaaa gatcttgaca ccggttttga tattgtattt attttttagc
     8821 aataaagcag cttgataaac attgttccgt tcatctaccg gtaaaaatgc tttatttgtt
     8881 tctacaatga ttttgtcttc tgttatttct tcaaaaaata gatgatctgc caagtctacg
     8941 ctcgacatca ccatttccaa ctcatgatac ccatcttgcc gtctatataa tacatccagt
     9001 cctaaattta tcttggctgg cgctttttca atcaattcca tcctctcacc cattcgaaaa
     9061 gtctaagtcg caagtattct atcataggga aaaataaaag tatagcttat gtctgcctag
     9121 tggttgattt tgtcatcgtt tcaacagctt tcacagaatt ttctcttcat aatccaaaca
     9181 agttctagtg attcttataa ttctgtgagc acttcttctg ccaaactcgg aacatttcct
     9241 ttttcttttc ctatcagact gagtagctgc atggtagcaa atccgaaatt acttcctttc
     9301 ctataagcaa aataacgtcc ttcccaatga tcgacatatt ttaatttctt ttcataagca
     9361 gactggaagg aatagaaaaa attaccataa ttatatacga tatgcatgac tttttcttga
     9421 aagaaagaaa aatctgtttc tccaacatta gctaatggag ctaagcctag attggcttcc
     9481 gagtagccca ttttctcgta tacaataaac agattggcta acagatagtc agctaattcc
     9541 tcagattctt taggtaagac ccgcaacaaa tcatatcccg cctgcttctt cgtcatcggc
     9601 tgagcggtga tgaagccaac gactttttga tctttacgga caataccgac accgctagat
     9661 aatacatatg atttatcaaa tcgtcccaca gagaagtatt tttctcgtgc accttgaagc
     9721 catttttgtg aaacttcatt tacagcttga tgtaattctt ccggcagttg atcgtaccaa
     9781 gtaaaatggt atccttcttt catcaaacgt tgatattcaa gttgctgact cagccgagac
     9841 aagtcttctg ttgctagatc aacaatccct gcttcaccta ctttcgtgaa gtgaaaaccc
     9901 aattcatgaa gcatcactac gtagctttct gatatcttat aaaacgcaag ctgatagcct
     9961 aaacaatctg cttgtttcat aaactgattc gttgctgccc gcatcatttt aggattgcca
    10021 actggatctc ctaaaacaaa gcatttgttt cctttgatct gataaccaaa aacgacttga
    10081 tcttggtcgt cttcttggta ataaaaataa ctgtaacctg acaagcgaag ctgatggctt
    10141 gtagctgtgc ctccgtatgt ttccaataaa cggtcaaaac ggtctttttc gtactcactg
    10201 ccaagcttcg tagtatcttc agctaaatat tgataaagcg ccactaaagc tagcaatgag
    10261 atgcccaaac caataagccc tgagaaccac acatcatctg aaggaaacaa aataaaatga
    10321 tttcctgtta ttcgaccgac taaaaagcgt ccgctgtgat agcctgcaat tgcgtaaacg
    10381 ataaacaaaa agccgaacaa gctagcatca aagattatag ctccccaaga ataaacgaat
    10441 tttgttcgat aaaattcttt tctggataac cacacagcta acagaatcaa gacatatacc
    10501 aatatcaact gccaagataa cgtacgtgtg attgtattta aaataccaaa agatagtaaa
    10561 gcaatcgtag gcagaaatgc ctttttgact tttactgaaa gtgctctagc aaggccgagt
    10621 aataaaaagc caaccaatag attcactgtc tgatctaaaa aattgaatga gaacggcaat
    10681 aatacttgaa atagccgact gattgcagac agattagtga ttgttgatag aagtaccatc
    10741 atgatccccg caaaatagac agcacttacg agcacgatat gcgcaacctt ttggaaaaac
    10801 agtcgcggaa tgttatctaa aaatcgattg attttgactc ctgtatgtgt gatgaacaat
    10861 agaagtcccg tcataaaagg tagaacataa taaaacaacc gataatataa tagccaaact
    10921 actgctacat cttgccgaac aggcaattga ctcattccta agatcatcaa aacgtcaaaa
    10981 gtccccattc cacctgggac catcgtcagc attcctatca atgtagcaat catgaacata
    11041 ggatagatcg aaagcatcga aatatctatt ctcatcaact tgccgatgat caaaaatacc
    11101 gcaagggctc ccgtccattg tccaaaggat gccagtagca gaccaagcaa gccttttgga
    11161 taaaactcga agaacaatgt ctttctcttt agataagcaa aaaagaaaat gactggtgct
    11221 aaaaaactac cgccgaaaag ccagatccaa tagcttcgaa aaatactttc tgactgaata
    11281 aaaaagacat caataaatgt aagaaacgac catacagaca aaccagtcag cataaatagt
    11341 gcgacttttg atacagtggc taaaaccatt tttctatttg tctgctttcc ataaaaacta
    11401 gcccgcagac ttgctcctac cacaccacca aaaccggcga gattgttgat cgtgtttgtt
    11461 acccacgcag caagaagaaa ctcttttctt tccatttttg gttttccttg tttttctaag
    11521 actctgatcg tcacccaatc atacaacaac atcggagtga cacccagcag tccagccaga
    11581 atcatgagca ggatcgtcat gcggctttgt tgtcccatcc tttgccaaat atcttgccaa
    11641 ctcatgccat tagcaatatt tgcgacttga tttgccacaa agactaacac ggagccgaaa
    11701 aaaatcagtt ttaaaatcag actatgttcc tttatccatt gcagcatcaa gtatcgttta
    11761 tttttcacat tttcccattc ctaacttgta tacttttgtg tcatacccca atgttatctt
    11821 atttatttta atttttccac tttagacctg ttctttgctt gcgatgatag aaaaagaaag
    11881 atatcctaat gctaaaggag aggataacat gaaagtagga tttatcggca cagggaatat
    11941 ggcacaagct attattgatg gtttattaaa aaaaggaaca gtctcgaaag aagaggtgtt
    12001 cgtttccagt ggacattttg aaaatgcaca aaaatttgct gctgaaactg gcgtccatgc
    12061 atgcaaaagt aatcaggaag ttgcatcaca atcagatatc gttcttttag ccgtgaaacc
    12121 aaaaatcatt gaaaaagtat tggaagaact aagagaatat agccagaaag tcttattcat
    12181 ttctattgct tcaggaaaat cattaacaga tttagaaaca gcgcttgaag atcctcaggc
    12241 ctccatcgta cgtgtgatgc cgaatgtcaa tgtcagcgta ggagcaggcg tttctgcgat
    12301 ttgcaaaaat gaagctgcca cggaggaaca gcttcagcaa gctacgatga tttttgaagc
    12361 aatcggcagc gtctatcctt tagcagaatc agatttcagt acattcattg gccttgctgg
    12421 cagctcaccc gcattcgttt acatgctgat cgatgcgatg ggacggactg gtgtactgaa
    12481 tggtttgtcc aaacagacag ccactgagat tgctgcaaaa gcaattatgg gttcttgtca
    12541 aaaattcctt tcaagtgata aaaatccatg ggaacttgta gaccaagttt cttctcctgg
    12601 cggtacaact gttgcaggtg tagttgcctt agaagaagca ggattcatcc cagcaatcat
    12661 caaaggaatc aatacgacga tcgagaaaga tcttgagatg ttgaataaaa aataatgata
    12721 gcaatgtttt ttcgtgcctt acaaaaaaag aagagctgtg gcttttgccg cggctcttct
    12781 tttgtatttt ttattattta gcataatcag ttgcacgagt ttcccgaata acgattactt
    12841 tgatgtgtcc aggataatcc agttcttctt cgattcgttt acgaatatca cgtactaaac
    12901 gaacagcgtc taaatccgag atttcttctg gcttgaccat tacccgaact tcgcgtccag
    12961 cttgaactgc gtaacttgat tctacaccgg cgaagctgtt cgaaatattt tccagacttc
    13021 ttaaacgacg gatgtagttt tctaatgatt cacttcgcgc accaggtctt gctgctgaga
    13081 gcgcatctgc tgcagctaca aggacagaga taacagaagt agcttccaca tctccatgat
    13141 gagaagcgat cgcgttgatt actactgggt tttctttgta cttggcagct aattccgcac
    13201 cgatctcaac gtgagatcct tcaatctcat ggtcaagtgc tttcccaata tcatggagca
    13261 acccggcacg ttttgctaat tggatatctt cgccaagctc tgctgctaag ataccagcta
    13321 attttgcaac ctcgaccgaa tgcttcaaga cgttttgtcc ataactagtt ctgaatcgca
    13381 gacgtccgat gatcttgatt agatctggat gaagtgtatg agcaccgact tcaaacgctg
    13441 cctcttcccc gtattcgcga atacgttcat ccatttcttt acgagatttc tcgaccattt
    13501 cttcgatacg cgctggatgg attcgtccat cttgtatcag tttttccaag gtcatccgtg
    13561 cgatttctct tcggattgga tcgaatccgg aaagaacaac tgcttccggt gtatcatcga
    13621 tgattaaatc gataccagtc agcgtctcaa gcgttcgaat attacgacct tctcgtccaa
    13681 tgatacgacc tttcatttca tcattcggaa gggtaacgac tgaaacagtc gtttctgaaa
    13741 cttgatctgc tgcgcagcgt tgaatcgcta atgataataa gtttttcgct ttgcgatctg
    13801 cttcttcttt ggcacgctgt tcagattctt ttaccattag agttaactca tgattcagct
    13861 cttcttcagt cgatttcatg atgacatctc gtgcgtcctc ttttgataaa ccagcaattt
    13921 tttctagttc tttttgttga tcttcaatca ttttttcaac gtctttttct cgctcttcaa
    13981 ttaattgctg ttttgaacta agtgcttctt ctttactttc aagtgagttc tcgcgttttt
    14041 ctaacgagtc atctttacga ttcagcgttt gttcgcgttg aatcaaacga ttctcttgag
    14101 attttaattc taacttactt tctctcagct cactttcaac ttcagaacgg tatttctgat
    14161 tttcttcctt agcttctaac aatgcttctt tttttagagt ttctgcttct ttttttgcac
    14221 tatcgatgat tccagcagct gatgattgtg caccagctat ttctttctcg tgacgtgact
    14281 ttgcgaccat aaaccctaaa ccaagaccga caattaaacc gatgatagcg aggagaatgt
    14341 tgaataccat aaaatccacc tccatactat ctttttcatt tttatcatct atacagcttc
    14401 tatgataaat tattctaaac tcgtcaatat gcatcttcgc atatatgtgt acaaaaaaca
    14461 tgcacaactt tattctaatg tttgtacccc gtagtgtcaa ctttaaataa acaaagaaac
    14521 ggctttctac ttattcatta aacgatggtt tttaaaataa atgacactag tttcatatag
    14581 gtaaaccttt aaattgagaa acttttctat taaattattc tttgtagatg aacagatatt
    14641 tttattttat gtacccactc gtataattaa ataactcacg atttctttat tcaataaaaa
    14701 aacggcaggt caaggagagt acctactctt cagaccagcc gtttttgttt atcataattt
    14761 ttattcttct actaaaggca attcttcttg tcctttttca tcttctggga cagcgacttc
    14821 ttcaccaata ccataagctg ctctaactag agcagaaact tctgccatca tttcaggatg
    14881 attagccata tagattttag cgttttcccg tccttgacca atccgatctt ctttataaga
    14941 ataccaagca ccgcttttat caacaatgtc tttttctact gccatgtcta gaagttctcc
    15001 ttcttgtgag atccctaaac catacatgac atctacttca gcgattttga aaggaggtgc
    15061 tactttgttt tttacgactt tgatctttgt acggttacca acaatatctg taccttgttt
    15121 caattgttct gcacgacgta cttccaaacg gatcgttgcg tagaatttca atgcgcgtcc
    15181 tccaggagtg atttctggat tcccaaacat cacgcctact ttttcacgga tttggttgat
    15241 gaaaatagcg attgtttttg ttttgttgat cgaaccagag agtttacgca atgcttgaga
    15301 catcaaacgt gcttgtaacc cgacatgtga gtcacccatt tcgccgtcga tttctgctcg
    15361 tggaactaaa gcagcaactg aatcgactac tacgatatct acagccccac ttgatactaa
    15421 agcatcagcg atctctagac cttgttctcc tgtgtcaggc tgtgaaagaa gtagttcatc
    15481 gatattcaca cctaattttt gtgcatattg cggatctaac gcatgctcag catcaatgaa
    15541 agcggccgtt ccgccatttt tttgtacttc tgcaatagcg tgtagtgcaa ctgttgtttt
    15601 acctgaactt tcaggtccat atacttcgat gatacgtcca cgggggtaac cgccgacacc
    15661 taaagcgaca tctaacgcaa gagagccact tggaattgta gagatttgtt ggtcgatttt
    15721 ttcgcccaat ttcataatag agcctttacc atagctcttt tcaatttttt tcagtgcagc
    15781 atctaaggct gctttacgat catctgccaa tacgataatc ctccttatat ttacaaatca
    15841 gagcaacatt tcattatcct tatcatatct tttttagtga aaaaaagcaa gaaaaaaccg
    15901 aacaaatatt cgcatttatc attttcttaa aagctgtttt ctcaaaagat caaatccttg
    15961 cataaccgca ttatatctga tagcagcacg atctctggca aagtgatatt tttccgaaac
    16021 cacgccgtct ttagatgaaa tagcgatcca tactgttcct gcagattgac cttctaacgt
    16081 gtccggtcca gccactccgg tgaaagctac actgtaatct gttcttgcta tctcacgtgc
    16141 ttgaatggcc atctgctcca cacattcttt gctgactgtt ccatattctg ctaatagctc
    16201 cggatcaatc tccagaaaac cagcttttgt ttggagcgag taggtaacga atccgcctgg
    16261 aaatacttca gatacaccag caatgtcgcc taatgcggct tggaaggcgc cggctgtcaa
    16321 actttccgct gctgttacag tctgcttgtt ttctttcagt agttccacta cgacttttgc
    16381 tagcgaatta tcatcgccgt atccataaaa atattcccct actcgttctt ggattttttc
    16441 ttctaatgct aaaagagctt gattccctgc ctgaacatca tttgttttca ctgtcagtcg
    16501 taatgtcact tcatttggtt tggcatatgg tgcgatcgtt ggattgatct gtgtctcaat
    16561 cagatctttt agctcagtga ccagtcgcga ttcaccaatc ccataaaagc gcaaaacttt
    16621 agaaattagt ttttcttcag aaggaaaacg ttcctctagt aaaggacgta cttgttccac
    16681 aaacattggt ttcaactcat taggcggacc gggaagaagg atatacgcat gtgtatctgt
    16741 ttgataaaaa gtccctaaag caagccctgt acgatttggt agaggaaccc cgccttcgat
    16801 aatctgggac tgttgcagat tgtttttggt catttttcga tgggttgttt caaaatatgc
    16861 caataatttt ttgtatcctt ccgtattttg tatgagagac ttaccgaggt gagccgcagt
    16921 tgcttctttt gtcaggtcat cttctgtcgg tcctaatccg ccacataaga gtatgagttc
    16981 acttcttgtt tcagctagag caatcaactc ttctaaacgc tgctgattat ctcctacaac
    17041 tgtttgataa taaacttcga tacctagatc agctagctgt tctgacaaga aagtggcatt
    17101 cgtattcacg acttgtccta gtaaaagttc tgttccgaca gctattattt ctgctttcat
    17161 tacttcccgc tcccttatat agtatacgct gtatttttat atttttttat ttaaaaagag
    17221 ggcaaggagc tgtgacaaaa atcggttcta taatattttg tgttggtgga aaatcgaaag
    17281 cgattttcta ctaacacttt tttcttcgtt tcaactcaaa aagagactca cttttggtaa
    17341 aatttagtcg accaaaacgt caccctacca aaggagtctc tcaatggata atcatactag
    17401 aaaattactc ggattaacag ataaacattt attttttgat gaagaatggc tgattgaaaa
    17461 agaatataaa ggcgttcaag cgcagtttat taaagggaga ttggatgact caccttccca
    17521 ttgtgaacac tgtggatcga ttcgtattat ccgcaacggt tcctatacga cacgcactca
    17581 gatgttgaaa gtcaaagaaa agttgactat tttagaatta aaacgtaccc gttttctctg
    17641 ccatgattgt ggacggacct tttctgcgaa aacggactta gttgatgaac atcatcaact
    17701 aacaaaggaa ctgaagcagg cgattctcat ggaactgtac gaaaatcaat cacgtaaact
    17761 aatcgctaag aagtacttcg tctcggatgg gacggtgaca cggatcttgc gtgaagcaac
    17821 gaagcattac cagccacgta tgaacttcct tccgacggtg ttgtgtatgg atgagttcaa
    17881 atcaatgaa
//
LOCUS       DANKKG010000051        17683 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000051 DANKKG010000000
VERSION     DANKKG010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17683
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.051"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            71..901
                     /locus_tag="PW004_001426"
     CDS             71..901
                     /locus_tag="PW004_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HDL2726408.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(1145..1463)
                     /locus_tag="PW004_001427"
                     /pseudo
     CDS             complement(1145..1463)
                     /locus_tag="PW004_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028215.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1585..1779)
                     /locus_tag="PW004_001428"
     CDS             complement(1585..1779)
                     /locus_tag="PW004_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726409.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(2284..2469)
                     /locus_tag="PW004_001429"
     CDS             complement(2284..2469)
                     /locus_tag="PW004_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726410.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(3319..3660)
                     /locus_tag="PW004_001430"
     CDS             complement(3319..3660)
                     /locus_tag="PW004_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDL2726411.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(3660..3890)
                     /locus_tag="PW004_001431"
     CDS             complement(3660..3890)
                     /locus_tag="PW004_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDL2726412.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            4177..4515
                     /locus_tag="PW004_001432"
     CDS             4177..4515
                     /locus_tag="PW004_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726413.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(4720..4959)
                     /locus_tag="PW004_001433"
     CDS             complement(4720..4959)
                     /locus_tag="PW004_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HDL2726414.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(4935..5282)
                     /locus_tag="PW004_001434"
     CDS             complement(4935..5282)
                     /locus_tag="PW004_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HDL2726415.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
     gene            complement(6014..6595)
                     /locus_tag="PW004_001435"
     CDS             complement(6014..6595)
                     /locus_tag="PW004_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2726416.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(6778..7404)
                     /locus_tag="PW004_001436"
     CDS             complement(6778..7404)
                     /locus_tag="PW004_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL2726417.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(7426..8757)
                     /locus_tag="PW004_001437"
     CDS             complement(7426..8757)
                     /locus_tag="PW004_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HDL2726418.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(8912..9313)
                     /locus_tag="PW004_001438"
     CDS             complement(8912..9313)
                     /locus_tag="PW004_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HDL2726419.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(9399..9605)
                     /locus_tag="PW004_001439"
     CDS             complement(9399..9605)
                     /locus_tag="PW004_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726420.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(9700..11202)
                     /locus_tag="PW004_001440"
     CDS             complement(9700..11202)
                     /locus_tag="PW004_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HDL2726421.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(11220..11675)
                     /gene="argR"
                     /locus_tag="PW004_001441"
     CDS             complement(11220..11675)
                     /gene="argR"
                     /locus_tag="PW004_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HDL2726422.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(11773..12390)
                     /locus_tag="PW004_001442"
     CDS             complement(11773..12390)
                     /locus_tag="PW004_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HDL2726423.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            12611..13984
                     /locus_tag="PW004_001443"
     CDS             12611..13984
                     /locus_tag="PW004_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2726424.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            14059..15171
                     /locus_tag="PW004_001444"
     CDS             14059..15171
                     /locus_tag="PW004_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDL2726425.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(15243..16880)
                     /locus_tag="PW004_001445"
     CDS             complement(15243..16880)
                     /locus_tag="PW004_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HDL2726426.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(17048..17656)
                     /locus_tag="PW004_001446"
     CDS             complement(17048..17656)
                     /locus_tag="PW004_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL2726427.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         4989 a         3618 c         3103 g         5973 t
ORIGIN      
        1 ctttctttga attttttaat ctttcgtagc agaatgagaa tgtattaaaa atagattagg
       61 aggaattttg atgtttagac accagaaaga attgcagttt gaagcaaaac cagatcgtcc
      121 gaatccatta atcgcgaaat atctgcagga gctgatcggc ggccaatatg gcgagatgag
      181 tgtagctatg cagtacctgt tccaaggctg gagctgccga ggagaagaaa aatataaaga
      241 tatgctgatg gacattgcaa cagaagaaat cggccacgtg gaaatgttgg cgacaatggt
      301 tgctcgttta ttagagggtg cgcctgttga agatcaagaa gcagcgatgc aaaaagatcc
      361 aacaatcggt gccattatct ctggaatgaa tccacagcac gcgatcgtag ctggcttagg
      421 acctcgtcct gctgacagcg tgggcaatcc atggagcggt gcctatatca ttgcgagtgg
      481 caacttgtta gcagacttta gagctaattt aaatgccgaa tcacaaggaa gacttcaagt
      541 agcacgtatt tacgaaatga tcgatgatcg tggcgtaaaa gatatgctgt ctgttctcct
      601 tgcaagagat agctatcatc aaaatctatg ggaatgtgca gtgaaagaat tagaagaaaa
      661 agaaggttca atcttagtcc caagtacttt cccgcgtgac aaagaacgaa aagacattgc
      721 ttatgacttc tataacttct cagagggaga ccaaagtagt gaaggttctt gggctagtgg
      781 caaagcactg gatggcgaag gcgaatatca atggttgaaa gaacctaaga tcgaaggcgc
      841 tgcaccaaaa ttagaaccag taacaccaaa aatgtatggg acacctccat tcaagaaata
      901 aatatctacc cgtagaaaca cccgtttttt gttccacacg ctttgtgagc aaaactgact
      961 aaagtcatta atcataacca cccttcaaaa gggtggtttt ctctagagct ataagctcaa
     1021 atcaccggcc agcgcctaaa gacgctggct ttcatatttt atgttcaagc catattgcct
     1081 ttgccagttt tgcccacctc ttaaaaggtt tatgtactac tccttagccc ttaaaaggat
     1141 cgtcttactc tcgtacactc agcttatcta acgcactatc atgtttttct tgttcttgaa
     1201 tgtacttttt gatggtcgct tcattttgtc ctacttttgt cacatagtat ccttccaccc
     1261 aaaagtgtcg atacccaaat ttatatttta gatttgcaca atctcttgat gatctcctga
     1321 atattggctc gatattgatt ataaattatt tttcttctat actttggtgt gaatacaatg
     1381 tggtatttac acatctactt tgtacgggct aaactatttc cctttattgc tccacccgca
     1441 tagcaagtgg ttttgtattt caatcgctcg gcgcgctcaa ttaggtctaa agacaataaa
     1501 tctaaaaacc cgctgcttca ctgacagaaa caacgggttt tcagaaaaaa atgaaatgaa
     1561 aaagatctct tacggcaatc tattttattt ttttgatttt ttataaatgg aaaatgcttc
     1621 aagaagtcca agaaataaag ccgtataaaa acaagaacct atgaaatctt tatttattag
     1681 atattcaaca gaaatcccga taacactagc caacaatata tttatcagaa gtgtaaaata
     1741 aggatgcttt ttatagaata gaactagtgt tgttttcata gactctcctt tcttcatatt
     1801 tccaattaat cacaaattca cggcattact agaaaaatta aaggggcgca gaagtggaaa
     1861 aatactcttc gactttccct acaaaccagg ccatcttgtg tttaataact ataccaaatc
     1921 taaggtacct ctaaatctaa attccatttg tggcggtgac ctccaatttt taactcttct
     1981 atctcccatc cagtatacat aaccttgata cttcgaccac ttatatatcg aacacacaaa
     2041 tacctatcca ttgcaggagt actacctaac ttagttgcta cttgcatcta aataacagct
     2101 gaaaagatag gacttactct tagtaaagta tctgaactat taatttttaa actatcattt
     2161 ttatcctcaa ctacatgcac atctgttgca acttcacttt ctgtctcatc cagataaatg
     2221 attaaggaat cgggaattgc aatatcttca gctgacgctg gaatagttga gctaaattag
     2281 tcatcatatc tttattataa aataaccaac agcaaaaaat acaatgaaaa acaaacttgg
     2341 aactaatgta tcattaataa atgttgaaaa attcagcaac tcattactgt atgattttag
     2401 cagcccaacc aacatatacc ctataaaagc taataagatg gttaggaatc ctttagaatt
     2461 tttactcatt catagacact tccttaaata acttttatta tagaaggtat tgtaaataat
     2521 aatattgaca aaaagtgact caaaataaaa aaaaccttac attatactgg aatgaactgt
     2581 attgatagtg tacctaaaag ctctactgac ataagatcgt gtaatcggta aagtaatttt
     2641 attaattata cgggatgtaa tcaattagag aataaactaa tcataaaagg tacttttaca
     2701 ttcataattg tcgctttgag attctacttt ggttcactat ctgacccaat tgcacatttt
     2761 cgatcattac aagtattggt gtcgttagaa caactaaata aaaactagcg gtagattttt
     2821 cagaatctat gctaatttct gtatggatgt tccagtgatg gcaaatttct cttttcattg
     2881 tttttcttgt cgcatgatat gcctttttgt gttattcagt taaactatag aaattctttc
     2941 ctagacagat cttgatccca aattttacgt ttttgagcat cgatatagtt taaaatgaca
     3001 ttactcatat ctaaagagtt tagatttttt aggactaaaa ctgtcagttg ttctttttct
     3061 tctttctgtt aaagtattgt cgcgactttt cgtatacgtt aagatatgtc gtgaaaaaac
     3121 aagttttgtg acacaaaaaa acgaatgact tgaggaaatc acctcaatca ttcgtttaaa
     3181 atgctatata ttctttagtt taagatacgg aaattaatgc catcgaccac actagtagaa
     3241 tgaacgacat ttacttggcc tttttttaat ttcgttatgt ttagcatata aaaatattat
     3301 agagtttact tacaatgctt aaaacatata ctgtataatc tgatcaattt tagccatgtc
     3361 ttgcaatggt aagttttcta tttttttgag ttttcgttcc ttaaaatcaa gagattttaa
     3421 ttgtgtaata attacttgtc cttttatatc tagctcttca ggtaatgagt aacgtgtcgg
     3481 taaattcttt atagtggaag taattggaca tatgaccgaa aacattgttt tacggttaaa
     3541 ttcataacga gagacgacta gtcctggtcg tcttttttga atttcttttc ctacggaggg
     3601 atcaaaatca atccatacaa tatcaccttt tttaggaata taaattcttt cttctgacac
     3661 tataaaattt ctctcccttc aggagttaaa tcttcccatt cgtctttctc ataatactcg
     3721 gcctcttccc caccactaaa tggatcttca atttttggaa ctaacgtaat tgttccatca
     3781 tcagaataca caacgatata ttcttggttt tctgaaggtt tctttccgtt atcagaaggt
     3841 aaagttacca ctacagaact tccttgtaat ctagattttg taacaagcat tatcatcact
     3901 cccttaataa aagtatacta tataacacta agtaacgcaa aattcccctt tgtaaactat
     3961 ctagaaaatt gttttgttta aagtatatct tattggatat aaatagaaat aaattgtagc
     4021 cagttcattt tttctttaac taaaaaaata atccaagaaa cattgaacct atggatccaa
     4081 aagctaccca aatactatag ctaacccccc gctaccttca attttgcaga taaaacgctt
     4141 tgggagaaaa tttcaaagac aacaggccat cctatcatgc ccgattataa acaaaaagct
     4201 ggctattcct tttgctctga caaatttcaa ttgggaaagt tcacgaaact agcaggtgaa
     4261 gaagtttcaa ctgttcggat caaagaatgc ccgcttcaaa tagaaacatt ggttcaacga
     4321 atttcaacta gacaagactt tgctattgta gaacgtgaga tcgtagctat ttttgtagaa
     4381 gaagggattt tacatgatcc aactcatatc aatgtggata aatggcaacc tcttatctat
     4441 aaattcagag aatatacaac tgccaaccag tcgttaactt tgaattttag attccaagaa
     4501 ttcaaagagt cataatgata aacatcgtta ggtataaata aaaaagactc gcagcacgag
     4561 taaaaaacaa atagaagcta tacattagaa aggtcgactg tgaccctata gatgcagtat
     4621 atgcagctta tgagcatttc ttctgttctc tttgtttttg ggatctgtgc tgaagtcttt
     4681 ttcgtttttt gccgctcttt ctttgaaaaa tcgattatgt tataccttct ccattactaa
     4741 ttcaggcatc tgctgagtaa tgatttttcg cgcaaaagaa atatctgtta cccaaggttc
     4801 gatttgttct tcagtcaaga caagcggcat tcggtcatgg attggtgcga ctgtgcggtt
     4861 aggttcagta gtcattatga ttgattctgc cattttttca tcctgatggc tacgataaaa
     4921 accgccaata tacactattt tttccttatt tgaaaacata aatttgtctt tttcttcatt
     4981 ccactcattg ggaaaacaca acgattttgc aaaaatggtt gatgaaaggt ctttttttct
     5041 tctactgtct ctgcacgggc attaatcatc aattgtcctt ttttaaatcc ggtaaatccc
     5101 cacttcatga tttccggagt gaggatgttt tcttcctctt ttcttaacgt cacaacaaga
     5161 tttgacggaa aaacttctcc agctgctaat tctgctgcct gcttcggttg tttcgtctgg
     5221 gcttctcgat aataattctg aaggagctct gactctagat caaaaaaata acgaccacac
     5281 atcctaatcc ctcccaatct ttttttctat catacactgc aacaagaagt tcgcaccaac
     5341 aaaacgcttt caatttttgt agaactaaaa aagttgtgac aagtgacaaa ctctccagtg
     5401 aatggtattc tcctgccaac tcctcggtca caagtcacaa tattcaacaa acatgagaag
     5461 ctgctttttg aaattgttcc ttgtgactcg gagtgaaacg gcaggcttac gacctctggg
     5521 taaacggtgt tcaaaagcca gtttctcggt attaagccaa atttatgcaa aaatgtagaa
     5581 agctattttc acaaaatttt tcttaatact cgaaactaag cgactttttc acaacctctt
     5641 ccttaaacgg tgttcaaaag cttgcatctg cggtaataag cccaaccaaa cgaaaaatat
     5701 gagcagctat ttttatttgg ctgttcttat tacttgatgc agggcagctt tttcacaacc
     5761 tctccactaa acggtgttca atcagctaac cttcggtatt agctcaaaaa acggcaaaat
     5821 atgaggaact attttgccgt ttgtttctct aatactcagg tcaaagcgct gatttcacaa
     5881 cctctaatac acggtgttcc agaagtagct tcttcgaaaa ataagccgaa atttcacaaa
     5941 aatttgagaa gcaattttcg taaattcctt cttatttctt gaagctgacc acttctgtca
     6001 caacctctaa ttattaaaga accttttgcg aagttaacaa tctttctttg atcaattgaa
     6061 aattctgcct aagtagtgat agatcctgtg tgactttcaa caataagata cttccctgga
     6121 tttcagcaat caacgctttg gcttgacggt ctgcttcttc atcggacagt ttttcttcag
     6181 aaagtgccag gatattttgt ttcgtatact tgacccattg attgactaat tctagtagca
     6241 acagacggta ttcttcatcc actgtactca actcaataat caaattacct acaggacagc
     6301 catgataagt gatctgtgtc tcatgatcag cgaaaatcca gtccaacatt tcttctaatg
     6361 cttcttctgg ctgttttcca gcatttaaga tgtcttcgaa caattctgtc tgccatacgt
     6421 ccatatgcgc atggatcacc gctagacaaa cttctttttt tgaagaaaag taataataaa
     6481 attgaccttt tcctgtatca gaagctgcta gaatatctcg gattgacgtt tcatgatagc
     6541 cttttttata aataagatct ttggtcgctt ccaagatctt ttcctttgtt ttcaatgata
     6601 ttctcctttt tcatttctca tagtcctata ttatacagta aattcatcat tttttcttct
     6661 taatcgttta aaaaagactg tgccaaatta tctgacacag ccctcactat aaaactgttc
     6721 tcatatagct agatgttcat tgattcaaga actgcgagga caaaagcaat tcctatctta
     6781 tgatagttgc tctaatactt cctctgttgt catcacttga tctgcaataa agccaaagtt
     6841 cgtcaacgct gcatccattg cttcttggcc aggagcacca attgcatctg tcgctaccac
     6901 aacattgaat ccactctcta ccaattcacg catatgggaa tcgctacaca agtttccgtt
     6961 cattccacca agaattactg tgtcgattcc attttttctc aattggagcg ccaaatcatt
     7021 gctttctggt ccaaagattt tatgaggaga gctgatgatc gtattttcat ccaaataagg
     7081 tttcaattct tcgataaaat cggctcctga accttcttcc actgattcat attgactttt
     7141 tctagcaaac atctgctgct caagcatcat atgttctcct gcaccgccaa attgccattt
     7201 atggtcatgt gggtaaaaat aatgaggaga aataaagagt ttgtatcctt tttcttttgc
     7261 ttgttttacc atttcaatca tattttcgat cgtattgtat ttttctaaga tatcttttgt
     7321 caaaccaaag ccaccgcctg ctggtttcaa gaactcgact tgcgggtcag tgagaactac
     7381 tgctgtattt ttattattga acatggtgga ttctccattc gttatttatt ttaatacatc
     7441 atttagagct tcactcattg tcggatgaga atagatttga tctctcaata acgtataagg
     7501 aagttttcca ttcatagcta gcgaaatcaa gttgatgact tcatgagaat cttccgcata
     7561 gatacttgcc cctagaatca agtcagtttc agggtcgact aaaactttga atacaccttt
     7621 aggatcttct aatacttgtg ctttagggac accgcttgtc atatacttga ataatttgta
     7681 attgacgcct tgtgctttgg cttgtttttc tgttaaacct acattggata aagtcggagt
     7741 gataaataca ctgtaaggca cagctttccg gttattcgtt gttcgtttat tctcgccctt
     7801 caactgatcc ataatgattc gataatcatc tagagagata taagtgaact gcaatccgcc
     7861 tttgacgtct ccaatcgccc aaatgttgtc tgcatctgta cgaaggtatt catccacagc
     7921 gattgctccg cgatctgtta gtttaacatc tgtattttct aatccaagtg atgctgtact
     7981 tggttttctt ccagttgcag ccaaaacttt tgacgcttgg attgttactt cttggccatc
     8041 ttctgtaaag gtgacggcag ctgttgattc ttggtctgct acttgatcta cagatacacc
     8101 tagatggaat tcgattcccg catttgtcat gtcttctaaa atcatttgcg cgatatcttc
     8161 atcttcacgt ggaagaaatt cagaatgggc atcaagaacg acaactttag atccgtattc
     8221 attgaacatt gatgcaaatt ccaagccaat atatccagcc ccgatgatca ctaatttttc
     8281 cggtaattgt tcctgatcca tcgcttgcgt agaatcaatc aagtagcgac ttgtattcaa
     8341 tcctttgatt ggtaaaatga ttggttcaga accagtattg atgaaaatcc gttcgccttc
     8401 aacaatcact ttttcaccat tattcgtcac ttcgatttga tggttagaaa gaaaacgtgc
     8461 tgtaccgtcc atcactgtcc cagttgcctc atcgctgatc atatgataat ttttggcgcg
     8521 aagcatgccc gtcaattttt ctttccgagc aacagcctct gtaaagtcga ttcctctttc
     8581 tccattgaaa atcaatgatt tagaaggaat acagccgatg ttgatacatg ttccgccata
     8641 catacgtgtt gacttttcaa cgactaaaac cgattcacct tttgaagcta acgccttcgc
     8701 taaagtctta ccgccctttc caaaaccgat cacaatattt tgatattttt ccatcatttt
     8761 tccctcgctt cttttttacc taccgattgg tacaaaaata tgataaccga taattgtcta
     8821 ttctccaaat catctgcttt atttgtcaaa aaaaaccaga caaaaagcgg tcctttcacg
     8881 tttttagtct ggttcgattt cctttattct tctaaacaat cgttacttct acatcgatat
     8941 ttcctctagt tgctttggag taagggcaga attcatgcgc atcttctgca attttttggg
     9001 cttcttcttc gctgatgcca tcgatatgca cttctaatac tacaccgatc tgatagccgc
     9061 catcatctaa attataaaga gagacacgtg cttttactgt ggactctgct gtcattcctg
     9121 cttctttgat catatgttgt accgccccat tgaaacaaga agcataacct gctgcaaata
     9181 attgttctgg atttgttcct tctgcatgga ttcctggagg agtgattttc atatgcatcg
     9241 agccatttgg tgcctcgact tctccattgc gcccgccatg gttgatcatt gctgtttcgt
     9301 atgctttttt cattgtactg ctcctttcga ttgaccattt gctacttggc tataattata
     9361 gtgtatgaca agtaaggcgc tttctacaaa aattctgctt attcgaatac cagaatgccg
     9421 tcttctacta tttttccaaa aaacaaaaaa atactactcg tgaacaacag cgtcatattt
     9481 gttccaaaat aaatagcaat cttttctaca taatctttta tatgattgat tttaagatac
     9541 atatatacca gaaagttgac cccataaacc aatactgtta aaaaaataac catattcgtt
     9601 accatttttt cctccttttg ctggtctccc taccagcact cgatttaacc ataccacttt
     9661 ttttgccgtc caagcaacta gcctttcaaa aagactggtt tattctgctg ttaatgattc
     9721 aagatcagaa aaatgtccga ataattttgc taattttact aacgccaact tgttctttcc
     9781 gaattcatca ttttctatct gtaatactag taacggctca tgtaagctta aacggatcaa
     9841 aaaccagccg tttccgtatg gctcttctag atcatatcga ataccttctt cattttccgg
     9901 gtcgaagttc atcccttttt cttcttccaa cagtcgcttc atttcttcta atatcttgtt
     9961 tccttgttcg cgtggttcat cgctgaacaa ttttaatcgg atttcttgta cttctacggg
    10021 ctgtttcaac tctttgattt gttcatctaa tgtgatacct tttcttttta attctggcaa
    10081 aagcatcagg atctttgcga tgacatatgc accatcatcc agaaaatagt tttccttgaa
    10141 tgcagcgtgt ccgcttgttt cgatggctag ctgtgtatct ttcccaagac ggttaagttc
    10201 taatgcttta ttgattacat ttcgatagcc ggaaatataa cgaacttgga tgcctccttt
    10261 ttcttcaatg aatctttgta gatgagaaga agtaggggaa ttcgtcacga ttcttgttcc
    10321 tggatgctct tttaatacga ttgtacttaa tactgcgatc aaattatttc gattgatcac
    10381 tgttccagaa gcactgacaa ctgcagaacg atccacatct gtatcgaaaa tgatcccaag
    10441 atctgcttca tttgctaaaa cagcctgctt gatactttcc atcgcttctt tattatcagg
    10501 atttgggata tgattaggaa atcggccatc tggttccaaa aattgggaac ctgttgaatc
    10561 cgcacctaag ctattcaata ctttttcagc aaagaatcca ccagcaccat ttccggcatc
    10621 aacaatgatt ttccaaccat ttagtggttt gctttctagt gttccaatcc cctttttaat
    10681 ttttgtgact aaatcttgcg cataaggtgt caaaaggtct gctttttcta cgcttccagt
    10741 ttgttcgctt tttatttttt gctgctgtga taggataaaa gcaatatctt ctttttctgc
    10801 tcctccttct gaggtaaaga attttattcc attataataa tagggaagat ggctagcagt
    10861 cagcataacc gcagcatcgc acgagaactg ttcaaattga gtggacataa acatagcagg
    10921 cgtagtagct aattcgaaat cgatgacatg tatcccttgt tctttcagac tttggatcaa
    10981 cgcctctttc agtgctggtc cgcttatcct actgtcatga ccaatcccaa tcgtccattt
    11041 tttatttgga tcggttgatt tcttcttttt ctttatccag tttgctagtc cccagccgat
    11101 cttttttacc atgtctgtcg tcaagtttac ttcatatcct tcaactggta atgcgattcc
    11161 tcgaatatcc gatccatttt gcagcgcttt catatctttc atataaaagt cctcctcatt
    11221 tattccctca ttttttccag cttttctgca aatcgctgtg cttcttcttc tgagcggcaa
    11281 ataataatga atgtatctac accggccatc gttccgacaa cttcagggta agccacttca
    11341 tctagccaat tcgttaccac atcggcacca tttcgttcag tcaatacgat aaccatgaac
    11401 tgaacagact ggatacgcaa aacctcgcgt ctaattgctg tacgcaaacg ttcttcattt
    11461 aaagaaacag atggctggtt aaataatgta tagcgtacta ttttgttttc attttgactt
    11521 tttactaact tcatttctcg aatgtctcga gaaatcgttg cttgagtcac atcggcgcct
    11581 gcttcatgca gccgcgcaag cagctcttcc tgtgtcccaa taggttgttc tgtaatgatc
    11641 tggcggatca gtgcttgtcg ctgtttcttt ttcatattag acacctctca atgttgatta
    11701 tagcaaaaga taagtaatat ttcttcatat tttggtattt tttactttta ttttttgttt
    11761 tcattcaggt tgctaacctt tttctatttt aacacgcaaa atcgtcttca ttttttcagg
    11821 tgctgatttt cttggatcgc tgatgtaaat ctctttgtgt tctttagaag ctctttcata
    11881 gccttctttt gcgatgaatg cttctaattt ctcaaacgtt gctggttctt cggcataggg
    11941 cccgacgtgt aagatttggg cacaaagacc ttcttccatc tttttgaata ctacttgttc
    12001 tttcaagtct tccggaagtt tgctagaacg ctgaatggct tcttgtgcta tcgcttctgt
    12061 aacaaagtca ggctgtcgga tcatcaattg ataagaaaaa tgttcttttt tcatcactgg
    12121 ttcatgcaaa tacttttctt gcagtcccca ttgtccttcc agtggataga cagtatacat
    12181 ctgataatca ggaatcagcc aatttttttt aggcgacatg cgtattgcat agctgactgc
    12241 atatagacag gctactcttc gtttaaagtc ttcctgattc gggtcgccga tccctctgat
    12301 gctaaagaat gattttttag gaacctctag tatttgcggg ctttttttca tactatagct
    12361 cttgtcttta cgccattcaa ttttttccat ttttcttcct ccgcgaaatt ccatttagtt
    12421 atttattatc atacctcttt ttgagtgact tggctaatcg tgttgacact atttttcagt
    12481 ttgtgctaga ataagtgaag taaataaatc gtacagataa cacgtttcgg cgtgtcgaga
    12541 gagaggcggt ttctattttt ataatagaaa tcactctctt tttttacgaa attttttaga
    12601 ggtgatgtaa atgacaaaac aaaaacagct gcttttagta cctgcagtaa ttgcaacagg
    12661 gatgatgtct tttgcgggcg tattgatcga gacggccatg aatgtaactt tcccaactct
    12721 tatcagacag tttgatttaa cgactgcaca agtccaatgg gtaacaacga tttatctttt
    12781 gatgatttcg atcattgttc cgctgtctac ttatttgaat cgcaatttta gtctcaaaaa
    12841 actcttttta gcttcaaatc tgcttttctt ggcaggtata atgatcgatt tcttctctcc
    12901 taccttcatg gtcctattgt ttggtcgatt gctccaaggt gcagcgacag gtatcgctct
    12961 tccgttgatg ttccatatta tcttgacatt tgcccccctc aatcgacgag gcgcgatgat
    13021 gggtgtcggt actttgacaa cggccattgc tccagcaatt ggccccactt atggaggcat
    13081 tatgacttca cattttacat ggaatcatat cttcctgttt cttgtgcccg ttttgtttct
    13141 ttctttgatt atcggctggt ttgctattcc cgagatggca gtacaaaaaa gtggttattt
    13201 ggattggata agcgttttag gaatttgtct tttgtttagt ggaagtctaa catttttgag
    13261 tacgttggga acagtttcca gctggattgc tttaatcatc ggcggtattg gctggttcct
    13321 tttttactat cgaagtaaaa aagtggagca tccactgatc cgactgtcta ttctttcaaa
    13381 taaaacattc cgcttgtttt tgattggctt cttagtttat cagtttttat tacttggtgt
    13441 atcttttgtc ttgccgaatt tccttcagat cgttcaagga agtagtcctt ttgtcgctgg
    13501 attagcgatg cttcccggcg cagcggttgg tgctttgctt tctccttttt ccgggaaaat
    13561 gctggatcaa tatggtccga agaagccaat ttttgctggg ctgattcttt ctttgattgg
    13621 ttggctagca ttattcctac tcataggaca cgcttcagtg ggtgtattga ttgcttgtca
    13681 tgtgttttat atgatcggta tcggattatc ttacagcaat atgatgacca ccggaatgaa
    13741 tgcactgaat gaagaactac aaggagacgg gaacgcaatg ttcaatacct tgcagcagtt
    13801 ttctggcgct gtggctactt cattagtagc ggtcattatc aattacgtcc agcatcatac
    13861 ttcccataac tatgaagtgt ctactacatt aggatcaaaa gcagcattag gcgtcttgtt
    13921 gctgttgctt ctcgtaagct ttgctcgatt cgcctattat ctgttctttg ctaaaaaagc
    13981 gtagattcca tatacggcag gtgttgcatc tgccgttttt ttgtgtgaaa atggttgttt
    14041 gtaaaaggag gcttttttat ggaaaaaatt gccattatcg gtggcgggat cattggtatg
    14101 accctagcca gttacttaga tacatcaaaa ttcaatgttg ctttatttga tgaagggaca
    14161 ggacaggcaa ctaaagctag cgctgggatt atctcccctt ggttgtcgaa acgcagaaac
    14221 aaacaatggt atcgtctggc taaagacggc gctgcttttt tccctaaact tataaaagat
    14281 ttccaacttg atcaaacgat ttacgcacaa tcagggacac ttttgcttcg acctgaaaaa
    14341 gatttagctg aattggctgt tcttgccgag aaacgaaaag aagaagcgcc agaaattgga
    14401 aacatcgaat ggctttcacc tgaacagaca tctcaagcac ttccgttatt gaaaccagca
    14461 gtttcactgc gtatcagtgg tggtggtcgt ctggatggaa aagcatattt agaacatctt
    14521 ggtcagcgtt tagcacaaaa acaaatggag cagatccagg caaaagtcac tttgcataag
    14581 aagagcgatt cttggctcat caagggaccg tttggagaat acacagctga tcgtgtaatt
    14641 ttgacacctg gcccggcact taagccttta ttacaagaat taggctatgc tgcagatata
    14701 cgtccgcaaa aagggcagct cttagcattt cagacatctt ataagatgag tcaagactgg
    14761 cctgttgcta tgcttgacgg agaagctgat ttcattcctt tcatggatgg aaagatcctt
    14821 ttaggtgcta cccatgaaaa cgatcaaggc tgggatttgg ctcctactag cgaagcttat
    14881 aagggattga ctaagagtac agaacagttc ttgactgatc cagatcagat aaaaaactgg
    14941 ccatacgagt tccgtgtagg tacccgtgct tatacgtcag atttttcccc atttttcggc
    15001 gtactgccag atgatccaag cttactggct gctagcggtc taggctcttc tggcttgacc
    15061 acaggtcctt atatcggcta tctattagcc aacttcttaa atactggtca ttggcctacg
    15121 gaagattatc aaaaggacat ctccacctat attacgaaaa atccatcata gaaaaacagc
    15181 tttttcaagt aaggatcccg agcgatattg cgcatggatt acttcttgaa aaagctgtta
    15241 ttttagttca atttctgaat caaattctcc aaactttctt ttgcttcatc tggcaagaga
    15301 tcttttccta tctgagcttc aatgaatcgt aaacgaatat caagtcgttg tttcaccaga
    15361 tttttatagg acgtatcctc aaaagaaaca ggaaaatctg gtataggtaa atagtggatg
    15421 tcttcaaatg gaccaaacga cttgaattct gccgtttgtt gcacgattct ttcaacgatt
    15481 gacaatgtcg tttcaggttt gattttttta tcatcgaaat atccagtatt caggacatag
    15541 caggctgttt gctgtttcaa aaacaactct cgaaaagctt gatagtcttc cctaagagga
    15601 tagacacgga aaggattagc aaatggttca atgactagct catctagctg tctttttgta
    15661 gtgatatttt ccgcgtctga tcgttttgtt gctaatgttg caccaaaaga cgctgctaaa
    15721 actgggtcat tgatcttgat aattggcgga agcgaatcat ctttcatgat ccaaaaaaca
    15781 gcatcgatct tttcgctaag atgatcgact cggtcgggtg tcgcataacg tgatttgact
    15841 gtccgcccat ttccgtttcg tatatctcct gttactagga ctttttctcc tttacttgta
    15901 catgtcacgc ccacattctg acaagtaagg aaagaagaaa tggccggatc atcagacgga
    15961 tagtcttgtg ttttatcaaa gtaagaaggt tccaaggcag tagcaaaacc attttttcga
    16021 gaaataacga aagcatcgtc atgcaagatc gaaacagagt gaccttccgt atgttttgct
    16081 aaggtaatcg ttgattttcc agaaccggac aacccaaata cagccatcgt ataggtctgg
    16141 tcattactag catattgttt catcccaccg tggcaggcaa taaagccatt gcgatttccc
    16201 aatgcccaag caagcgtcaa agtcgctttt tttaattctc caaagtaacg taatcctagc
    16261 acagccgctg tattatggag agcatcaaac aaaatgagtc catttggaaa tgtttcatgt
    16321 gtccattcgg gatcacaaaa gatataaaga tctccctcat caaaggaaac agacttttga
    16381 taagtctcta agatcttcga attcgctggc tgaaaattca gcaaataaga atacaaattg
    16441 ttctcaaatc cttctggaat cagcaaatga ctttgcacag aaaattcttc agataggcca
    16501 acgattactt ttgtatggta aaactggcgc ttgcgacttt gatacacagc ttctcttaaa
    16561 attttctcgt atttgctttg ttccttgtct aagtcctcaa agatccgccg agcacttgct
    16621 gttcttccca caatgcttcc atcattgaat actagtgtcc taacatcagc tggtaaatct
    16681 agttcttcag catgtgagat tgttaaatct gtgatgatcg ttcctggact cccttttgct
    16741 agttgataag ctgtcttcct atcaaaaact tcatgaacat tattaccgta aaatgtcgtt
    16801 tctacaatcg atcgaatact ggataacagt ggatttccac ttgtgatttc gtttaattcg
    16861 agactctctt ttgtactcat ggctcctcct cattccctaa acaaaaaaac gttttcttta
    16921 ttatacatta ttcgagtttt tatttgtact gctgcgctgt gggaagcaaa cgagttattt
    16981 ttcacacaaa aaaaccgaga acttgaataa acaagtttct cggtttcatc attttccttt
    17041 tatcggctta gtaccagccg tttgctaacc agaatgtttt agcagcatcc catgaaccat
    17101 aacgagatgt tacatattgt tctgctacac gttcttgatt ggcagctgaa tagtcgccgt
    17161 ttaagtatga agcatctaat tgataacggc cgatgtaacg tccgttagta gctgtgtatg
    17221 aaccgcctga ttctttttgt gcgatccatt cttttgcaga gcttgaagtt ggcgctgttg
    17281 cagcagctgg ggcagcagga gctacttcaa ctgtttcaac aggtgcaacg tatgtatgtt
    17341 cttgcgttgc tgtttcagtt gtagatgctg tattttctgt tgttttgttt tctgttgttg
    17401 ctgaagaatc atttgaagtt gggattgtta attgttcacc aacataaatg cggttgatgt
    17461 ttgcgatcga atttttttca gcaattgcat cgatcattga gttgtctcct gcaaattttt
    17521 gtgagatctt agataatgaa tcacctgatt ttactgtata aacttcatca gcgtgagcat
    17581 ttgtagttcc tacaaacatt gcagtagcac cagcagcaaa agttgtacct aaaacaattt
    17641 tcttaaatga attcatatta aactcctttt tttctccgtt ttt
//
LOCUS       DANKKG010000052        16026 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000052 DANKKG010000000
VERSION     DANKKG010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.052"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            226..894
                     /locus_tag="PW004_001447"
     CDS             226..894
                     /locus_tag="PW004_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HDL2726428.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1324)
                     /locus_tag="PW004_001448"
     CDS             complement(1094..1324)
                     /locus_tag="PW004_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726429.1"
                     /translation="MTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGE
                     AYEDYFMIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1451..2833)
                     /locus_tag="PW004_001449"
     CDS             complement(1451..2833)
                     /locus_tag="PW004_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HDL2726430.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2989..4176)
                     /locus_tag="PW004_001450"
     CDS             complement(2989..4176)
                     /locus_tag="PW004_001450"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HDL2726431.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4183..5214)
                     /locus_tag="PW004_001451"
     CDS             complement(4183..5214)
                     /locus_tag="PW004_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HDL2726432.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5260..6684)
                     /locus_tag="PW004_001452"
     CDS             complement(5260..6684)
                     /locus_tag="PW004_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2726433.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMED"
     gene            6859..7764
                     /locus_tag="PW004_001453"
     CDS             6859..7764
                     /locus_tag="PW004_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDL2726434.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7855..8532
                     /locus_tag="PW004_001454"
     CDS             7855..8532
                     /locus_tag="PW004_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL2726435.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8529..8699
                     /locus_tag="PW004_001455"
     CDS             8529..8699
                     /locus_tag="PW004_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726436.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8813..9709
                     /locus_tag="PW004_001456"
     CDS             8813..9709
                     /locus_tag="PW004_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL2726437.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9764..11134)
                     /locus_tag="PW004_001457"
     CDS             complement(9764..11134)
                     /locus_tag="PW004_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2726438.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11372..12118
                     /locus_tag="PW004_001458"
     CDS             11372..12118
                     /locus_tag="PW004_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDL2726439.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12115..12954
                     /locus_tag="PW004_001459"
     CDS             12115..12954
                     /locus_tag="PW004_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDL2726440.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12955..13905
                     /locus_tag="PW004_001460"
     CDS             12955..13905
                     /locus_tag="PW004_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HDL2726441.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14046..14798
                     /locus_tag="PW004_001461"
     CDS             14046..14798
                     /locus_tag="PW004_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726442.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14817..15968
                     /gene="queG"
                     /locus_tag="PW004_001462"
     CDS             14817..15968
                     /gene="queG"
                     /locus_tag="PW004_001462"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HDL2726443.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5201 a         3000 c         3228 g         4597 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttttcttg aaaatgccaa acatgttatc ctagagaatg ccggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt tgataccgtt
     1381 gactagttct tccatcgcaa aagcagtccc ccattttaat ccaaatggcc gatctcgata
     1441 ctcgttatag tcaatgaatt tttttgctgt ccgtctcatc ttttccacct tctaatccac
     1501 tcataccacc tgcatgcccg ccaacgagag aactacgtgc gatggcgcgt cctccttgca
     1561 agagactatg cgcatggatg attgattgaa agccgtattt ttttctaatc gtatcgacaa
     1621 caaaatcaac tttcgcttca tttatctgct tttcaggatc ttcaaaaagg ttaagctgta
     1681 aacctgtcgc gtatacgagg tcggaacagt tcacgcctac acgccgaata tcctggtagt
     1741 ggtaatgctg gtggaaaatg gtcaataaag catctgcaag atctctgcta gcatttgtcg
     1801 cttcgatttt catttgctgg tgaaacccgg tcttgccatt tggatcaaga tagcctaaag
     1861 agtatcctac ccataagctg actacttgtg tttttgctgc agatcgtctc aagcgagtgc
     1921 cgacttggtc ggccatttct ttgataacga tctcgatttc ttctcgtctt gtataatctc
     1981 tatctaggat ttgactattt ccgatacttt ttgattttgg tgtatattct tgtcccaaaa
     2041 aacttcgatc gatgccccaa gcatgggcat aaagctgagt accgatgacc cctagcttgc
     2101 ttttcaagtg ataataatcc gcgtgagcta aatcataaac agaatgtata cccatttgtt
     2161 tcagacgttt tgctgttcgt ttgccgattc cccaaaagtc agtgatatct gagattttcc
     2221 ataacttttc tggtacatct tcataacgcc attcagcaaa catttgcttt ccgtcttttg
     2281 agccattgtc taaggctagt ttagcaagta gcgggttatc gccaattcct atagtcgtgt
     2341 agattccggt ctgctcaaac acatctttat ggatcagttt agccatgtcg tacgcatttg
     2401 ttttaccaaa gagtgccatg ctatttgtta catcaaggaa gctttcatcg acggaataga
     2461 catgatggtt cgcttcgtct gcatattttt tatagataga attgatttct ctgttctttt
     2521 ccatataata ggccatcctt ggggcaacaa tatataaatc acttggatag gggaagggaa
     2581 gatctctggc acggctgata ttggtgatgc cgtaagcttt ttttgctgca ggactggaag
     2641 ccaagattaa tccgctaccc cgtgtttctg tcgaatcgga cggataggac atgactacta
     2701 atttagttgt aaggggatca agatcctttt caacgcactc tacggaggcg taaaaagatt
     2761 tgcagtcaat acataaaata tctcttgaag gttctttttc ataaacaaat accggatttt
     2821 gattcgccca cataacaatc acctcttcaa cacaattaga acacacgttc tctttccgtg
     2881 tcaataaaaa acagcaattt tcaaatacga gcaaaaggct cacatttgga aattgctgag
     2941 aggttatttt tatctatttg tttttagctg agatcgttga tttttttatc aaacatgaac
     3001 agcttctgga tcgatctttg ttgtcccatt tccaatgtgt gcaacataaa attccggagc
     3061 atagccgaca actttcatgt attcatcata gacattgttt ttgaaggcag gtatattttc
     3121 ttctttgaca agagcaatcg cacagccacc aaatccagct cctgtcattc gtgcacctaa
     3181 gacaccttct tgtttttgag cagcatctac tagtgtatct aattcgatcc cagttacttc
     3241 ataatcatag cgaagagagt ggtgagaatc atttaacagt tgaccaaatg cttctaagtt
     3301 cccagcttct aattcagctt ttgccttcaa agtccgctga ttttcagaaa cggcatggcg
     3361 agctctgcgt atcaatgtat catcgccgat ttggtcaata gttgattcaa attcttcttc
     3421 atctaagtct cctaaagaag agataggcaa agttttttgc aaacgtgcca aggcttcttc
     3481 gcattcgcta cggcgttcat tgtattttga atctgccaat tcccgccgct tattcgtatt
     3541 catgatgacg attgcataac catctagttt cactggaacc atttcgtatt tcagcgtatt
     3601 tgtatctaat aagattgcat gatctagctc accgaagccg atggcaaact gatccatgat
     3661 acctgaattg acaccgatga attcattttc tactttttta cctgtttgga ctagttccaa
     3721 gcgatctgtt tttaaatcaa acagatcttc taatacaaca cctactaata attctaatga
     3781 agcgctactg gaaagtcctg caccattagg gattgttcct tccactaata actcaaaccc
     3841 atgatcgatt gagtgtccag cttcttttaa tttaaggacc attcctttta cgtaatttgc
     3901 ccagtttgct tttttatcat attctagctc atctaaagta aaactaatga caccgatatc
     3961 gtcaaagttt gtggaataaa ctaatatttt attatcgtct cgaggtgctg ctacaccttg
     4021 cgttccataa gtgatggaag cggggaagac atggcctcca ttgtaatctg tgtgttcacc
     4081 aatcaaattg atgcgtcctg gtgagaagta ttgtgtagtt tttttaggtc cgaatgtttc
     4141 ggcaaatttt gtgtttaagc ttcttttgat ttccatgatg agttattcct ttcttgtttt
     4201 gattttgaat tctgttactg tttcaaatac tgatccggct tttaaatgaa tcgaaccaaa
     4261 tgctgagaag cgctctgcac ctggagctgt ttgagtctca aatgtgatgc caccgtgatg
     4321 tgccagtttt cggttgcgca tttctggtgt ttctgtgccg aaatttgcag tgaaaataac
     4381 cacactagaa gcatctgtag caatatctac ttggattctt ttatcggggc tagtcaagcg
     4441 agcggcttct ttacccaagc cggtttcttt taagaaaaat ggatgatcga ttccgtcaac
     4501 aagatttttt tgatccaaat cactagcaaa aacggacgct aattttttag gtatttgaaa
     4561 atcgaaagca cttcctgcag cattttcttt tacccctata ggaatactat ctgttcgcaa
     4621 aggagcataa gcttcgctat tgagccaaag ctcatgttga tcgattgact gagaagcatc
     4681 tcccgtcaag ttgaagtaaa catgatttgt cggattgaat aaagtatctt ggtcactggt
     4741 gccgcgagag gtcactcgcc agatattatc ttttgtcaaa gtatagcgga tttccacatc
     4801 cattgtacct ggaaaaccat gttctccgtc gggacttgta gtagtaaaaa tgacagaagc
     4861 ttcattttcg ccattcaaaa tgacataaga ccattttttt aattcaaatc caggagcacc
     4921 accatgcaga ttatgtccag tttttggatc tgtggctaat tggtaagttt ttccattgag
     4981 agaaaagcgg ccattttcga ttctccctgc ggtgcgaccg atagaagcac cgatatacgg
     5041 atctttttcg atatattctt cagcggtgtc aaatcccaaa actaattctc gatcatttga
     5101 ttttaaactg acgattctag cacctaaatc agagatagaa agtgctagct tgtttttgtt
     5161 ctccaaggtt atcaagtgat aaccttggcc aaaatctttt tctgtaatct tcattttcaa
     5221 gatcctttct taatgggaag gaactactgc atcgtgaagt taatcttcca ttgtatcagc
     5281 tggagccaat ttgttttcca atttctcaac gatttgtgca tgttttttct ctgtcaatgt
     5341 aatcttccaa gcaaaaataa tagcggagaa aatcatcaaa gcagcaggag catagaatgc
     5401 ataagctttg aaagtcgtta cgccatgagc agtgatatca gcagcagtag cattaccagt
     5461 catcccagaa gcgatagcga caaaccctac gatcccgtta gacaaagcac cagcgatttt
     5521 atctagtaaa gggcgaattg atagagtaac agcttcgtta cggataccat ttttccattg
     5581 accatattca acagagtctg tgatcgtcat caaagcggat aagaaaatca attgttgagg
     5641 gaagtagaac aatactagcc cgatgataac gattggcaaa cttgaacctg cgatcgtaaa
     5701 gaaaaagtaa gaagaaatca tcattgcgat ccctccaagg taaacgtagc gacgagagat
     5761 gacgcgtgta agcattggga atagtggaac agctactaaa ccaagtaagg cagcaactag
     5821 accaacgatc caaaattcag ttggttggcc aagtacatat ttgaagaagt agaataatac
     5881 ggcagttgtt gctgcattgg caatcgcata agcgatataa gcaagggcaa gccacatcaa
     5941 ctgatcattt tgagcaactg ctttgaacac atctttgatc ccagcttttt cattttgctg
     6001 acggatcgca gtatcgactt cttttgtgcc caaggcagtg ataatcgcag taccgcctga
     6061 aataacagca gcaatcacac cgaaccagaa ccagcctgaa gcgccttcaa tatgtttatg
     6121 agtaacaaga taggtaaaga aagtaacgat agggacaacg actagagtag taccatttgc
     6181 acctagagaa gagccgaaac gagcaaacgt agcaaatttt tcacgttcct ttgtttccgt
     6241 actcaaagct ggaatcattg accaaaaagc gatatcctta aatgaataaa aactatccaa
     6301 taagatgaaa acgattgcaa ataatatgat aaataaagtc gtattatgag tagctaagcc
     6361 aaagaaattg gtaaagattg ctaccaagag tacggaacta acaacgccgc cgataactaa
     6421 ccatggtttg aatttaccgt attttgtacg agtattatcg atgatgccac caatcaaagg
     6481 atcaaaaata atttcaacta agcggatacc tacaactaag cttgtaactg tagcaatcat
     6541 ttttgcattc gttgccttat ctgttcctgc gaacatctgg cttgtcacga ataccataaa
     6601 ataagtgctt aatgtaacat agaatgtgtc gtgtccaaaa gcgcctaaag cgtaggacac
     6661 acgttggatc attttgcttt tcataatctg tattcctctc ctgttcaata tataatacta
     6721 agtaaaccgg ttacaacgaa taacaaagat cttttaatga cttaaactta acaagaaatc
     6781 gcttcctttt taatatattt ctgtgacttt tatttaactt tttgttgcat tatactaaag
     6841 gagtaaggag ggatagacgt ggagcaatcg atttttttaa aaaaggaaca tcaacaagta
     6901 gaaaaagaat tgtattttga tttttgtggt ttctctaaaa cgatgccgtt tcattctttt
     6961 ggtcctgcta ttcgcaatga ttacatcctg catgtcgtga tggaaggcaa aggatcttat
     7021 catgtgaaaa atcaacaatt ccaattgaaa aaaggtgatc tgttcttgat tcgtccagga
     7081 gactcgacgt tttacttatc agatggagaa gatccgtgga tgtattgctg gctttcattt
     7141 ggtgggagtg ctgcagaaga aatcattcgg cagtcgcttt tcagagaaga ccaatacacg
     7201 atggtctctt ctgacataca aaaatatatc gacattattt tagaatgcat gaaagatacg
     7261 gaagaaacct tggaaaatga attacagtta acagcattaa cctaccgttt tttatcagtc
     7321 cttttaaaag atggcggaaa ggtcagcggt ggtgtacaaa aggttcattc ctctttagct
     7381 gttgaaacag tcaaatacat cgaggcacat tatgctgaaa aattgtctgt tgaggaaatc
     7441 gcccagcatt tagcggtcaa tcggagccat ttgtcgcgag tattcaaaaa tcatatgggt
     7501 accagtatca aggaatattt gatcggggta cggatcaatc gaggggcgtt tcttctttct
     7561 ctgacggatg aatccgtgga aagtattgcc tatcaagtag ggttcaatag tctagtcgta
     7621 tttagccgaa tgttcaaaaa gagtaccgga gaaacggcta cgaactatcg taaacgtatg
     7681 aaaaacgaaa attttaagaa tattgctttg gataccttga aaaaacagtt ggaaaaacaa
     7741 accgcagttt cttggacaac ctgattagtc tggttaaaat gacaagcatc ggcaaaagaa
     7801 acttgtggcg gttatgctac actgtaaagc gaagactaaa aaggaggaaa agggatgcgt
     7861 acacttatca tcattagcca tccaacgatc gaagaatctt taaatcagca atttttcaaa
     7921 gaagcttctg ctggtttacc agctacttgg catcatttgg aatcaagtta tccagacgga
     7981 aaaatagata tagagactga actacagctc ttggcccagc atgatcggat cattttccag
     8041 tttccgtttt actggtatag ttctcctgct catttaaaaa tttggcaaga taatgtcttg
     8101 gaacgagcag cacatgtatt aaaagggaaa gaactaggaa tcgtattgac aacaggtgtg
     8161 gcggaaaaag aataccaagc tggaggaaaa gaagaatata cgatttccga atttttgagg
     8221 ccgtatcagc gaatcgcaaa taaattccat atgacctatc ttccgccatt cgttttagca
     8281 caatttatgt atttatccca ggaaaaacgt tgggagaagc taattgctta tcagcagtat
     8341 ctctcattgg aaggcaaacc atcgctgact cagcggattg actggtttat tcaaagagta
     8401 caggaaaatc agaaaatgca ggaagaagaa tctgaaaaac aagcatatat cgtagaagca
     8461 ctgactgagg caaaagagca gatcgaagac ttgaccttta cactgcaaga aatgaaagga
     8521 acttcattat gacaaatgat tggctgatcc aacaattaga tgaattaagc gaaaaatcag
     8581 aatatcaaga ccgtgcgctc ttatatgaaa taaagaaact gatggctgag atagacgaac
     8641 gaacaatgca gctgcaagga gaaatcgacg gacaatcttg gaatcatctc aactggtgaa
     8701 attttcttat aaataacaaa gataaaaatg tgcacagcga caattatttg ctacaataag
     8761 aatatgaagc gtttgataac gctattatac ataaaaattg gtggtgaaaa aaatgtatga
     8821 tgaaaatgag tggggagaaa atgttcggat gacccgaaga agacagcaaa aacaggcagc
     8881 tcgtaagcgg atgatcatga ttggggcagc agttgccgtg tttttgatca tagcaggagg
     8941 agttgcgggt ctatattttc atggaaaaaa ttcgtcagaa gcgaaaagta cgaccataac
     9001 ggaaacaacg acaaagaaaa cagagacgaa acaaacaagt gagtcatcaa ctaaaacaaa
     9061 agagacacaa acttctgaag aaaaaacagc aaatggtttg ccagagcaac ttcaagcaat
     9121 cgtaaatgat tattcgaaaa agatcgaaga aaagacgcct gcattgattg aagaatacca
     9181 gacagaaatt cagggaaatc aagagggaat agctggttta tcagcagttg ctaaccaaaa
     9241 agcacgtgag cttcaagcaa tctccgacga agggatcaga aaattacgtg cagcgtatca
     9301 ggcagcagaa aataaagacg gtgtagactt agatactttg atcaatcaat tatcggctaa
     9361 ttatacgaat catgtcgcta agatttccga tatttatttg cagactagcg cctctcttca
     9421 agcagagtca acaagcacac aagacacaac atcttctgat tctgagacag cagagtcggt
     9481 agaagcttct gaaatgcaag ctcgaacaac acaagacaca acagattcct ctgaaactaa
     9541 taccaacagc gcgacttcac aggctgcgac aaccgtagtt ttggaaggag aaggtccaaa
     9601 tcaaatagcc gaacgaactg gcgtaccagt agaaacgatc ttgtcgctga atgggatgag
     9661 catggagaat tatttcctga ctcccggtga gacgctaaaa ttgaattgat aaaagcaata
     9721 aaaaagaacg aagcttccac taaggtgaag cttcgttctt tttttatttt ccggttcttt
     9781 tttcgcgtac aaagtaatgg atcgtcatgt aaagcaagaa aacgagtcca ataatcaatg
     9841 agattctcgt ttcaggattg ataaacatga aaatcaatac gatagctaaa aaagcaagtg
     9901 tcacataatt cgtataagga gatagtggca ttttaaaagg atgatcatgc attttatctg
     9961 cgtgcttctt tttgaagcga atctcgctga tcaaaatcac tacccaagga accataccag
    10021 gcaaaatact tgagctgtat acgaaaacga aaatattttc tgactggttg atgaacattg
    10081 gtagaagata atttaagatc agaccaatta agattccacc tgagattgct acgacaggat
    10141 aaaaaggcgc accgtgacga gatagtttca gaaatttttt agacatcttt ccatttatgg
    10201 caagtgtata gaccatacga cttgcgctga aaatccctga attgcaacct gataatgccg
    10261 ctgtaatcac gacaaaatta atcagtcctg cagcaaatgt aatgccgact ttagaaaaag
    10321 tttgtacaaa aggtgaacca atctcactca attgattcca cggatagata ctcacaatga
    10381 taaaaatagc accaatataa aaaatcaaga ttcgaccgat agtggactgg acagcttcaa
    10441 tgatcgtctt tttaggattc tctgcttcac cagcagtcat tccgatcagt tcgattcctt
    10501 gataggaagc aacaacgatc gataatgcaa agaaaaatcc tttgacgcct ccagtgaaga
    10561 atccaccatt tttccaaaga tttgaaatcc ctactgcctg accatgatta ccaaatccaa
    10621 ataagattac cccaagacct gcaatgatca tcaaaatgat cgttatgact ttgatgatcg
    10681 aaaaccaaaa ttctaattcg ccaaacgcct tgacagaaat aagatttgcc agcataagaa
    10741 acaagacgac aatgattccg ggaataattg ccggcaaatc aggccaccaa taattcatat
    10801 aagatcctac agcaatcact tcacttattc ctacgacaat atattgaaaa atattactcc
    10861 aagcggtcaa gtagccagcc acaggatgga tgtactcgct cgcgtaattt gcaaaagaac
    10921 cagtcgaagg atcaacgtaa agcatttcgc ctaaagcacg cataatcaaa tacaaaaaga
    10981 atcctgcaat cgcataggct cccatcactg agggccctgt ccaacgtatg gttgaggagg
    11041 cacccataaa cagaccgaca ccgatcgttc cacctagtgc gatcatttgg atatgacggg
    11101 cactaagact tcgagttaac ttttcttctt ccatacaaaa actcctttcg ttgttgagtc
    11161 aacaagaaaa aataatcaaa cgtttcaaca catgtttttc attattaggt tagccttatt
    11221 gaactcttga tgtgattcta cagaaaattt tcataaaatg cgaggaaaat tcgaaaaaaa
    11281 ctttttcatc attctacttg aaaatgcttt ccattttagt taaactatat ttaaataaaa
    11341 agttaggtaa acctaaaaag gagtgtaaga tttggaacgt acggccattg taattcaaga
    11401 cctttcagcc gcttatcaag ggaaaactgt tttgaaaact atcaacttga cgataaatcc
    11461 acaaaaaatt accggtatta tcggaccaaa cggtgcagga aaatcaacat ttatgaaatc
    11521 tttgctagag ttgataccag ctactactgg taaagtcacc ttttctggac agtcggtaaa
    11581 ctcagtccgt aaaaaaatag cctacgtgga acagcgaagt gaattggatc tttcctttcc
    11641 aattgatgta ttaggcgttg tacttttagg aacatatcca tctttacgaa ttggacaaag
    11701 acctgggaaa aaagaaaaag aacgtgcaag acaagctttg aaaaaagtag ggatggaaga
    11761 atatgccaaa agacagatca gcgaactatc gggtggacag cttcagagag tttttattgc
    11821 aagagctcta gcccaaggag cagaatggat ctttttagat gaaccattcg tagggattga
    11881 tgcgttaagt gaacgaaaga tctttgacat cttgcaggaa ttgaagaatt caggaaaaac
    11941 gattttgatc gtccatcatg atcttcataa agtagaagaa tatttcgatg aggttattct
    12001 tttaaataaa cagctgatcg cttccggtcc agtacaagaa gtctttacat cgaagaacct
    12061 tcaattggcc tatggtgaga tcatcagaca tttagtaaaa ggaggagaga aaaaatgatc
    12121 caatccttta ttgatggatt aatgaattat caattcctgc aatacgcttt ggtcacttcc
    12181 atgcttgtag gacttgcttc tggagtcatc ggttctttta ttattttacg agggatgtct
    12241 ttgatgggag acgctatctc tcacgcagtt cttccaggtg tagccatttc ttatatgttt
    12301 ggttttagtt acatctttgg agccactgct tttgggatgc taacagcagc agtgattggt
    12361 ttcgtgactc agcggagccg attgaaaaat gatacagcga ttggaattgt ctttagctcc
    12421 ttttttgcat tagggattat cttgatttca tatgcccaga gtgcgacaga tttgtatcat
    12481 attttatttg ggaatgtctt agctgttagg gaaagcgatt tgttgctgac agcactcgtt
    12541 agtggaatcg tcttgatatt tgtctttttc ttttataaag aattgaagat cacatcattc
    12601 gatccgacaa tggcaaaagc ttatgggctg aacacatcgc tgatccatta tcttttgatg
    12661 ttctttttga cattagtggc tgtagtcagt ttacagacag taggaacaat cttggtgatt
    12721 gccatgttga tcacaccagc cgccacggct tacttgctaa cgaaccattt actgaaaatg
    12781 atcattacag ctgcaggaat cggtatgcta agtgcagttg tcggtgtgtt tttcagttat
    12841 agttacaatt ggccatcagg agctacgatc gtgttagcat gtaccgcatt ttttatcctt
    12901 gcttttttat tttctccaac aaaaggaatt ttatttcaga aaggaagcag atagatgaaa
    12961 acaaaaaaat cactcttttt aatcttggca gttagtttct tggtattagc cggttgcgga
    13021 aagcaggctt ctgaccaagc ggatgaagga agtaaagaaa agttgtcagt cgtggctacc
    13081 aattcgatct tggcggacat ggcaaaagag gtcggaacag atcaaataga tatccacagt
    13141 atcgtaccag tcggaacaga tccgcatgaa tatgaagtac taccagaaga catcaaaaag
    13201 gcaagcgatg cagatgttat tttatacaac ggtttgaatc ttgaaacagg taacagctgg
    13261 ttcgataact tgatggaaac ggctaaaaaa gaagaaggaa aagattattt tgcagttagc
    13321 aaaaatgtag aacctcttta tttaactagc ggtgaagaac atacaaaagc agatccccat
    13381 gcttggctag acctatctaa cggaataaaa tatgtggagg aaatcgcacg tatattctct
    13441 gaaaaagatg cagaaaatgc gacactctat aaaaaaaatg cagaagcata tgtggaaaaa
    13501 ctaaaagaat tagatacaca agcgaaggaa agttttgctt ctatcgaaga gaacaaaaaa
    13561 ttattagtaa caagtgaagg ggcattcaag tatttttcac gagcatatga tttgccagca
    13621 gcttatatat gggagatcaa tacagaaagt caaggtacgc ctgatcaaat gaaagcgatc
    13681 attgatcaga taagagcaaa agaagtaccg gttttattcg tggaaaccag tgtagactca
    13741 agaagcatgg aacgggtagc aaaagaaacg ggtttgaaaa tctatgataa actgttcact
    13801 gattccatag caaaagaagg agaacaagga gattcttact atcagatgat gaaatggaat
    13861 attgaaacga tccatgaagg tttgtcacaa acaaaagaaa gctgaaaagt gcaaggtcta
    13921 gcttgaggag cagaccttgc attttttata agagatatga aaaaaaatta tgctgttttt
    13981 cgtcgtacag ataccttttt tgaagaaatt ccgttatagt tagagtaagg aaggaaggtg
    14041 tggctatgaa ttttacacgt aaagataaat atacgattaa agaagaagtc tttcgattga
    14101 tcagccagca ggaaaatacc gacaaggatc aagaccttca atttcttgaa gcagaattag
    14161 ctaaattatt catagcaaat caaaatgaga caacagaacc agatgaagag ctgtacatag
    14221 gcaaatggtt gtttgatgaa tttcagcctt tagatgaaaa taccggtatc ggttattttg
    14281 tgttcctttc tccagtcggc ttcacacctg gcgtattagt taaacaaaac gacgaaataa
    14341 ccgtcaaagc atggcctgca ttattttcag tcatcaaagc agaaggagta atcaaaaaga
    14401 tccagtcggc ctattataaa agtcaattta ttgccaacag caaattaatg aatatgtttt
    14461 ataatcgtct agtagaaatt gctaggcctg ggaaaatccc gcaaacattc acgtacacac
    14521 agcaaaaaaa tctctatgaa gaagtattgt attcccgcga tctgtctgag ttttcgccat
    14581 gggaagaggt aaggctccat gaactgttaa gtacaaaacc agtttcattg aattttttta
    14641 atgcattaga taacaaaaga aaattaattt caacaaatta ttgggaagta tttgctgcaa
    14701 gaaaaaaatg gatggaagaa aagaagattg ccgatcagca gatccagttt gtccagctga
    14761 aaaaagaaaa actatattat gggttataca gccgttaaca gcaagcgagg aagaccatga
    14821 cattaaaaga agaaatcata caagaaagca aacgattagg aattgacaaa atcgggttca
    14881 ccacggcgga accttttgat tcattaaaag aatcgttgga agaacaaaaa gcagctggcc
    14941 atacttctgg ctttgaacat ccaaatatcg atgagcgtct ttatccagaa atgacatttg
    15001 atcagccgaa atcgatcatt tccattgcac ttgcgtatcc tactaggatc catgaagaag
    15061 tgcctaggga tgaaaaacgc gggcagtttg ctcgtgcttc ttgggggatc gattaccatg
    15121 atatactcag agaccgtttg aaccgattga tcgaattcat ccaaacaaga gcagaaaaat
    15181 ggcaaaaaga agaggaatgg cgtctagctc cgcaagtaga tactggagaa ctgatcgatg
    15241 tcgctgtagc acagcgagct ggtttaggat ttattggacg aaacggattg ttgatcactg
    15301 aagagttcgg ttcatttgtc tatcttggtg agatcgttac taatattgta tttgagcctg
    15361 ataaaccagg agaatttggt tgcggtgact gtatgcgctg cgtgactgct tgtccgacaa
    15421 aagcattgct tggtgacgga agaatgaatg cccagcgatg tttgtcttat caaactcaga
    15481 caaaagggat gatgccggaa gaatatcgta aaaaaatgcg aaatgtcatt tatggatgtg
    15541 atatctgcca gttagtctgc ccttacaatc aaggaaaaga ttttcatttt catgaagaga
    15601 tggaaccaaa agtagacgaa atatatccaa aattaaagcc aatgctttcg atgtctaata
    15661 aagacttcaa acagcaattt gggcatttag ctggttcatg gcgtgggaag aaacctttgc
    15721 agagaaacgc attgattgct ttagctaatc taggtgatcg aacggcattg cctgagattt
    15781 cagaatgttt aacagatgtc cgccctgtca tcagaggaac agcagcttgg gctattggac
    15841 gtctaggaac aaaagaaccg gaaaaatggc ttgatgtatt agatgaaata ttagctaaag
    15901 aaacagaaga agtcgttata aacgaattgg aacatgccca gaaattattg cggagaaagt
    15961 taaaataaaa aaatagttaa cattattcaa agtttttgtc cagtaattgt tgtaacaatt
    16021 actggt
//
LOCUS       DANKKG010000053        15985 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000053 DANKKG010000000
VERSION     DANKKG010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.053"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(50..1036)
                     /locus_tag="PW004_001463"
     CDS             complement(50..1036)
                     /locus_tag="PW004_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDL2726444.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(1059..1625)
                     /locus_tag="PW004_001464"
     CDS             complement(1059..1625)
                     /locus_tag="PW004_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HDL2726445.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(1639..2055)
                     /locus_tag="PW004_001465"
     CDS             complement(1639..2055)
                     /locus_tag="PW004_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HDL2726446.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(2512..4446)
                     /locus_tag="PW004_001466"
     CDS             complement(2512..4446)
                     /locus_tag="PW004_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL2726447.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            4727..5683
                     /locus_tag="PW004_001467"
     CDS             4727..5683
                     /locus_tag="PW004_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HDL2726448.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            5773..6876
                     /locus_tag="PW004_001468"
     CDS             5773..6876
                     /locus_tag="PW004_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HDL2726449.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            6889..7842
                     /gene="menA"
                     /locus_tag="PW004_001469"
     CDS             6889..7842
                     /gene="menA"
                     /locus_tag="PW004_001469"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HDL2726450.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(8777..10123)
                     /gene="gor"
                     /locus_tag="PW004_001470"
     CDS             complement(8777..10123)
                     /gene="gor"
                     /locus_tag="PW004_001470"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HDL2726451.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(10257..11510)
                     /locus_tag="PW004_001471"
     CDS             complement(10257..11510)
                     /locus_tag="PW004_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDL2726452.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(11516..12439)
                     /locus_tag="PW004_001472"
     CDS             complement(11516..12439)
                     /locus_tag="PW004_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDL2726453.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(12660..15152)
                     /locus_tag="PW004_001473"
     CDS             complement(12660..15152)
                     /locus_tag="PW004_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HDL2726454.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(15159..15626)
                     /locus_tag="PW004_001474"
     CDS             complement(15159..15626)
                     /locus_tag="PW004_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HDL2726455.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(15762..15835)
                     /locus_tag="PW004_001475"
     tRNA            complement(15762..15835)
                     /locus_tag="PW004_001475"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15799..15801),aa:Arg,seq:acg)
     gene            complement(15893..15966)
                     /locus_tag="PW004_001476"
     tRNA            complement(15893..15966)
                     /locus_tag="PW004_001476"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15930..15932),aa:Pro,seq:tgg)
BASE COUNT         4669 a         3336 c         2853 g         5127 t
ORIGIN      
        1 cgtagatttc cttctgagaa atttatttac gtatcgaacc tttccctatt cagtctttgc
       61 gatccaaaat cagttgggta agggaaaaga gattctcttt tgttcgaaga gaggtggcag
      121 gtaatttctt tatttccttc aaagcctttt ctgtgtaaga ggccgctaac ttcctagctt
      181 cttccacgcc tcctgtatgt tcaacgattt tgcgtacggc gtcgacatct tcttctgtca
      241 ttgccgcttt cttctctaaa taaggtagta gctttggttt gtttttttgc aaagagtaga
      301 tcaacggtaa agaatagacc ccttgacgca tgtcttctag tacaggtttg ccgatttcct
      361 ggctctcttt tgtataatct aaaatatcat cgataatctg aaaggccatc ccgatagaaa
      421 gtccgatatc tcctgctttt tttgcgaagc gctcgcttgt tccactttca aaagcgccaa
      481 cagaacaact taattggaac agttcagctg tcttacctga aatattttcc aaatattctt
      541 ttacagaaag ttcataatta tagcgatcat ccatctgccc taattcgcca ttcaatactt
      601 tttccatact gcgagaattc aattggagac ttttcaagga agtggagtaa tcagacaata
      661 gtttaaaaca gcagacaaat aaatagtctc ctgcatagac agaaacgaca ttaccaaact
      721 gactacggat attaggctga ccacgtcgca gatccgcttc atcaacgata tcatcatgga
      781 tcagtgtggc cgtatggagc atttcgatag aagctgccaa agcgatggct ttgtctcgat
      841 cttgctctgg tccaaattgt gagaacaata gctgataggc aggacgcaac agctttccgc
      901 cggcatggat cgtttctttg attgcttgtt cgacaggttt gtttttcagg cggacagttt
      961 cttccatccg ttttagtgtt tctgtcaatt ctttacttaa agcaggatag ttttcccaca
     1021 ttggatgtag cttcataaaa aaactccttg tgtgacgatt attttttggt aaacattggg
     1081 ctccattttt gtttggttcc ctgctcataa tctttttgga atcgttgaag tgtctgcaca
     1141 tgtcgtaaaa gataattcgc tgcgatcccg attgcaatcc ctgataaaat ccctaaaact
     1201 gaaaggactg gcaagtacaa catgactgtc catgattgtg caatgaaaga agccatcaaa
     1261 agctgtccga cattatgcat gaaaccgccg gccgcactaa ttccgatgat actgacacgt
     1321 tttggtccta attgcttgac caataacatt ccaaaataac taagcaaagc accactcata
     1381 ctataaagga atgtggaaat cgttccgcct agcaaagtag tcaggaatag tcgtagccaa
     1441 agtagtaaga agctgtcttg ttttttcatg gtaaaaatgg caacgatcgt gattagattg
     1501 gctaagccta gttttgcacc aggcgcaaaa gaaaaaggat aagggatcat attttccaat
     1561 aaaccaatga tcactccttg tgcaacaagt agcgaaatat aaatcatttt ttgtaattta
     1621 ctcatggtgt gtaaaggtct aataaatcag actgtcttcg tcacctccat ctgttgaacg
     1681 gacttcgatc actaatttat gtggcaaaca gacgatggtt tcgccatttt tcgttgccca
     1741 gcctcgacgt acgcagatct ggtcatcgca atctgcttct tttatccgta ctcgatcgcc
     1801 atctatctcg atcaagttat aatctccgtg ttcatctttg taagtatatg tgttgctctc
     1861 ttctccagcc atcaaaggga atgttttgat ttctgtgcca tccacacgca gtaccgcttc
     1921 tttttctgct gttgcggtgt tttgctgaag accaaaaatc atgactggta aaaaagaaag
     1981 aacagtcaaa agcaaaataa taaaaacatc ccaaggtttg agcagacttt tttcagcaaa
     2041 ttctttaagg ctcatttttg ttctcctttt tccagattaa ctcacccaat tttagcataa
     2101 aaacaagagg ttgtgacacc tgtgtcttgc tttgagtaat aagaacgaag aatagaaaac
     2161 agctttccat gttttctaat tctttgggct tattaccgag aagcagcagg tggaacaccg
     2221 tttataacaa gaggttgtga cagaagtggg caacttcaag aaataagaag gaatttacga
     2281 aaattacttt tcaagttttt gtgaaatttc ggcttatttt tcgaagaagc tacttctgga
     2341 acaccgttta taacaagagg ttgtaacaga ggttgtgaca cctgtgtctt gctcatggag
     2401 gcttatctcg ggcatcttat tcctttaatt ttacataaaa aggaggctgt gacggtttgt
     2461 ctcagcccct ttctattgaa atcttgcatt caaataacgt tctttatcta tttatttctt
     2521 gccgtactgg gaacttggtc taccgtacca ccaattacct agtgtacgtt gtagccatgg
     2581 aattacccag cgatccaacc caatcgcacg tccggaaccg ttcatcaatg cgaaagcaac
     2641 aaaaatgaac caaatgttta cccagtaaaa catgcctgat aagcagaacg tgatcaccaa
     2701 tacaatcgtt gtcgcactgc atagccatgt gaataatcca gcaatcaatg ctaatgccaa
     2761 ggcaatttca aagaatacca tgaatttttg gaagaacatt gctacatcat tgtttggcat
     2821 gatcacttgc atgatctttg tgaaccagtc tggtgacttc tcaatcacag ccattggttc
     2881 ttcaccgtat gcataactca aaccaaaatg tgcagcttga gtcgctgctt ctccggcact
     2941 tgcccctgct ccgctggcag ctgtggttgc atcaccagca gcagaagcac cactggcaac
     3001 atctgttaca ttgtattccc atgtccaagg gaacacactg tttccagtga accatgatcc
     3061 ttcaccaaac caagtacttg gtttgaacac ttcaccattc ccaatgattt ttttggaagc
     3121 ttcaacgaac caaactaaac catagaaaat acgaagtggc acactccata agacattgcc
     3181 gtaacgagaa gaatgtcctc gagtcacgtt gcgatcatct ttgatatgga aaaactcatg
     3241 cattaaatat tggaacatat aataaccaga acgaatatca aagaagtatt tcaaattcac
     3301 gatatgtttc attacgatag ctaaaaatcc gcttaaatgg atcttatcaa ataggttagc
     3361 taccccccat ttggcaccaa tagaaaccat aaatccttga tagtttcctt taaatttatg
     3421 cttttctccg cctttgatcg aagccacgat attcgctgcg gctgtgtgtc ctgtttgttc
     3481 agcagcttga acgatctgcg gtgtcggtgt tcctgggaat tcttcataaa agactaaatc
     3541 accaatgata tagatgtttt tatcttcgta gccttttgcc tgcatatatt cattggcgat
     3601 caaccgctgt ccgcgtgctg attctaatcc aaagtcagca gcgtcagaag ttcctttgac
     3661 accagctgtc caaatcagtg tgtgagtcgg aatgttgcgt ccgtctttca atttgatatg
     3721 atcagcagcc acttctacga ttggtgcatt caataataat tccacgttgt gcttacgcaa
     3781 atagcgttca gcttttgcag catcatttcg atccaacata ttcaagatcg tcggcattgc
     3841 ctctacgacc atcagtgtga tttcgtttgg atctaattta taatctttgg ctaggcgatt
     3901 tttccagtcg atcagttccc caatcatttc aataccggta aatccagaac cacagacaac
     3961 gaaagtcagc atggctttac gcagttcatc atctggttcc aatgcagctt ttgctactgt
     4021 cttctcaata tgttcccgaa tccttacaga atcttcaaat gaccaaaggg tgaatccgtg
     4081 tgctttgaca ccaggtgtac caaagtcatt cggctctccg cccattccaa ggatcagctg
     4141 atcaaaagga taggaaccta atttggtctt gacgattttt cgttctttat cgataccgat
     4201 tacagtgtct gtgacaagtt gtacattctt ttgacgagca aacaaatgct gcagatcgta
     4261 ttggattgct tctggttcta cacgtccgcc agctacttca tgcaactcgg tcatcatagt
     4321 atggtacgaa tgacgatcga tcagagtaat tgctacatct tcttttttca gtttctttgc
     4381 caagtatttt gtagctgaaa caccggcata tcctgctcct acaacgacaa tattttgctt
     4441 tgccattgat catccgctcc ttatagacta tctatataat gaatatatga taattctttt
     4501 agtttgatac ttattacaca atctccatat attatataga tgttctgttc ttttgtctaa
     4561 ctccttttgg atttttttca aattttaaaa taaagcgttt tttcacgaaa tttgctgaaa
     4621 gaaaacgttt gaaatattac atgggttatt tatcaaaaat agttgactta taggctaaaa
     4681 agattagaat gtataatgaa tcacaaagaa aaattgtgga ctagaaatgg aatggtgggg
     4741 tattaaaatg aagaaaaaac gttttgtaac tggatttgcc gtattagcat tttcagctct
     4801 agtgttaggc gcttgcggcg cagatgacaa cggaagctca aactcttctt ctgaatcatc
     4861 tacagcacaa tcttcgactg caaaaagcac aacagaatca tctgcaaaag ttgtagctgg
     4921 cggagatttg caagacggca catataaatt agaagaaaaa aattattcaa atggttaccg
     4981 tgcagtcttt gaaatggttg taaaagacgg caaaatcact gaatcaaaat atgataacgt
     5041 caatgaaaac ggcgaatcaa aaacaaaaga tgcagaatac aacaaaaaca tggaagcaaa
     5101 atctggcaca aacccagaaa aatacattcc tgagttgaat gaccaattct taaaagcaca
     5161 aagcgcaagc ggtgtggaag tcgtaactgg agctactcac tcatctgaaa gcttccaaaa
     5221 ctacgcacaa caattgatcc aagctgctca agctggtaac acagatacaa tcgaaatcga
     5281 caatggtgct gatttgaaag acggcactta caagcttgaa gaaaagaact actctaacgg
     5341 ctaccgtgtg caattcgaaa tgacagttgc tggcggcaaa gtaactgaat caaactttga
     5401 ttatatcgac aaagacggta aatcaaaaca agatgataca gaatataatg aaaatatgaa
     5461 agcaaaaagc ggtacagaac caaaaactta catccctact ctgaacgacg aattcgtcaa
     5521 agcaatgggc gaagaagatg gctcacctgc taatgtagaa gttgttacag gtgctacaca
     5581 cagctcacat tcattcatca tgtacgcaca acaattagtg aatgcagctg aaaaaggcga
     5641 cactcaaaca atcgaagtag ataatatcgt aacagaaaaa taatcacata atcatactaa
     5701 taaaagttgg agggtgggac gatagccaga ttaggcagtc gtctcacctt tcaaaatgaa
     5761 gagaggaaaa agatgcggaa aaaactacta ttttccttaa tcacactggg catctctttt
     5821 atggtgctaa ctggctgcag caactcagca aataaaacaa ccactgaaag ttcagcagct
     5881 accaagatat tgaaagatcc atattctgat gaacagttct tgttagggac ttacgttcgt
     5941 atccgtgtgt atgatgaagg caaagaatca gcattgaaac ctgccttcga tcgagtaaaa
     6001 gaacttggtg ataaaatcac gatcaatcaa aaaggttctg agatcgatga agtgaatgag
     6061 caagctggga tcaaaccagt aaaagtttct gatgacgtct acacgttagt caagcgggcc
     6121 tacgaatata gccaagattc tcaaggcgga ttcgatatgg cgattggtgc aatcacacaa
     6181 ttatggcgaa tcggttttga tgacgccaga aaaccttccc aagaagaaat cgatcaggca
     6241 ttgaaattag tggattatca taagatcgaa ctaaatgaca aagaaaaaac tgtttatcta
     6301 aaagaaaaag gaatgatcat cgatttaggt gcaatcgcca aaggttacat tacggacgaa
     6361 gtagtgaaag tcttgaaaaa acaaggagtg accactgcca tcgttgactt gggcggtaat
     6421 gtctatgtct taggccacag cccaagaggc gaaaaccaag actggacagt cggcatccaa
     6481 gatccgaaca tggctcgcgg atctgtttta ggttctatta aagaacggaa caaaacatta
     6541 gttacctctg gaatttacga gcgttattta gaagtagatg gcaaaaaata ccatcatttg
     6601 tttgatccaa aaacgggtta tccgttcgac aatgatatcg caagtgtcac gatcataaca
     6661 gataaatcaa ttgatggtga cgggttgtct acagcagtct tttcaatggg tgtgaaaaaa
     6721 ggactggaat acgtcgaaag cgaattgaat aacgggacgg aagcgatctt cgtgacaaaa
     6781 gacgataaag tatatgtgac cgatccaatc aaagacacct tcaaattatc ggaggactcc
     6841 cattacacga tgggtgaccg ttctgaacta aaataaggag tacgaaccat gacactaaaa
     6901 atctttttag aagtagttga aatacgaaca aaggtggcta gtgtatttcc ttttgcggta
     6961 ggggtattat tctctatcgc tttttttcat gagatacatt ggctaaacac ttttttattt
     7021 tttattggaa tgctagtttt tgatttagcg actaccgcta tcaataatta catggattat
     7081 gaaaaggctc attctgaagt atataagtat gaagaaaatg tcattggccg cagaaatcta
     7141 tctcctgtat tgattcgaaa catgatttta gctatgattg catttgtttt tgttatcggc
     7201 gttattctaa cgatcaatac gggttggctt ttacttttga tgggatttat ctgttgtttt
     7261 attggagtat tctatacatt cggaccgatt cctttatctc gaatgccttt aggggaaatt
     7321 ttcagtgggg tgactatggg ccttggaatt tttgccatgg tcgtttatgt caatacattc
     7381 ggttcagatg tctatgatct gacagtcaac ttttcatctg gacaatttgc tttggtagga
     7441 aatctttggg ctatagcagc aatcgtatta gcttccttac ccttggtgtt tacgattgcc
     7501 aatatcatgt tggctaataa tttgcgggat cttgaccgtg atattgaaaa tcatcgctat
     7561 acactagttt tttatatcgg acgtcctgcg ggaacagtct tattccagct attgatgtat
     7621 gcttgctatt tcgtgatttt aatggggttg ttgagcggta tctatgaatg gccgatcttg
     7681 gtcaccttct tgaccttgcc aattgtgaag aagcatttgg atcaattcaa gcaaacgtta
     7741 ccccagccta tcagtttcag ctattccatc aaaaatatgg tcttattcaa tgccagttat
     7801 gtattggggc tattactaac aatcattatg caagccattt aacagcatga agaggttgtg
     7861 aaaaaactgc cctgcatcaa gtaataagaa cagccaaata aaaatagctc cttatatttt
     7921 tcgtttggtt gggcttatta ccgcagatgc aagcttttga acaccgttta tcaggaggtt
     7981 gtgaaaaagt cgcttagttt cgagtattaa gaaaaatttt gtgaaaatgg ctttccacat
     8041 ttttgcataa atttggctta ataccgagaa actggctttt gaacaccgtt tatcagaggt
     8101 tgtgaaaaag tcgcttagtt tcgagtatta agaaaaattt tgtgaaaatg gctttccaca
     8161 tttttgcata aatttggctt aataccgaga aactggcttt tgaacaccgt ttatcagagg
     8221 ttgtaaaatc agtgttttgt cctgagtatt agagaaacaa atggaaaaat agttcctcat
     8281 attttgccgt tttttgagct aataccgaag gacagctgat tgaacactat ttgttcggaa
     8341 aaagtgacta actatgacac aatttttttc acagtccctt ttttcacttg atttgattcg
     8401 taccagcgaa cttgacgaga actttcttgg tttggtaact atatggtatt cttttaacat
     8461 cctcgtttgc ttcttctaat ttatttatac aaagtcacac ttttttaaac atctgttctt
     8521 cgatacattt ctatttcttt taaacatcga taccaatatt ttgaacttaa gagtgcatac
     8581 caaatggatt catttactct tcgtcagtta cgaagataat caaaatctcc aacttcccta
     8641 cttatttcgt tttgagaatt atcgaagcaa tcttacataa ataactaaaa aacaaaaaac
     8701 cagaaaatca gtatactgat tttctggttt tacatggttt gttcaataaa aagctgttgg
     8761 aactgttaat attaaattat cgcatcgtta cgaattcttc cgctcctgtt ggatggatgg
     8821 ctactgtatt gtcaaagtct tctttagttg cacccatctt gatagccacg gcaaaacctt
     8881 gcagcatctc atcgacacca acaccaatag catgtaagcc gacgattctt tcttcttttc
     8941 cgacacagat caatttcatt tcgcattttt ggcgataatc atttaatgca aagtacattg
     9001 gggtaaagcg tgaacgataa actttcaatt cattttcgcc atatttttcc aatgcttctt
     9061 cttctgtcaa accaattgtt gcaattggtg gatgcgtaaa gacgactgtc ggaacaagtt
     9121 tatagtcaag ataagaatct ttctttccat taaataaacg ttctgataaa cgtctaccag
     9181 ctgcgattgc aacaggagtc agatctaatt taccgatcac atcaccaaca gcaaaaatat
     9241 gttcatccgt tgtattttgg tatttatcta ctttgataaa cccttttttg tccaattcca
     9301 catttgtatt ttccagacct aatttttcgg tattcggtac acgtccaccg gcaaaaagta
     9361 cacaatcacc agttaaggtt tcaccatttt caaatagcac cgtatattcg tcattgtttt
     9421 tagtaatttc tttagcaata tgatgtgcgt aagtttttat tccagcttct tggtacattt
     9481 ggatcaaatt gtccgaaagc attttatcaa acgttctaag tggtcgttct tttcgatatg
     9541 cccacatgac ttcactacct aaaccacgaa atacacctga aagttcagcc gcaatgtatc
     9601 cagcacctaa aacgattgct ctcttaggta actctgttaa ttcaaaaaat ccatttgaat
     9661 caattgcgta ttctccacca ggaatggtca ttttacttgc acggcctcca gtagcgatca
     9721 agattttagg agcacgatag gtttcaccgt ttacttcgat cgtttgacta tcgataaaag
     9781 tagcgtaacc ttttatagcc tcgacatgat tgctatctaa tcctctttga taagcaccgt
     9841 gtagaaaatc aatatatttt tctcgtcttt ctactagacc ggcgaaatca acatttttta
     9901 aatctgcttg gataccgtaa cttgaggcat ctcgttcaat tgtttccaaa atcgtacttg
     9961 cttgccacat aactttttta ggtacacaac cgacgttgac acatgttccg cccaattcat
    10021 tcgcttcaat cagcaaaact tttgcgccat gcatccctgc tctgtttgct gaagcaatac
    10081 cgccagagcc gccaccaatt acgatgtaat catatatttg cattttgttc ctccttattg
    10141 aagagatcaa tcaaaacctt tatttaacga tcctttttct acagaaaaca agtgtaacac
    10201 ttttcttgaa ttttgtatgc gaagatgctt aaaacaggga tatatcagct ttttccttat
    10261 ttacgttttc ggatcatatc tccaggtttc accggctgtg cgactggcat agttaaaatc
    10321 atcattggat ttggtgctcg gtcaatggat tcgccttctt cattatgcat aacttcaacg
    10381 gtttgactga aatgactgaa tcctggacca taaaattcga tttcatctcc tacagaaaac
    10441 aagtttctct gacgtatcgt agcaatctgt gttgcctcat cataagataa tacttcaccg
    10501 acaaatttat atactgggat tttacgacgt tcaccgaata attgttcgtt ttcagttggt
    10561 gtattgtagt aaaaacctgt agctaattct cgttgtgcta ctttccataa ctcgtcgatc
    10621 cattcttgct tgcagacata gttttcagga tcttccatat aactgtctac agccgcttta
    10681 tagacatttg acaccgtaga tacataatgg atcgatttca ttcttccttc gatcttgaag
    10741 ctgtctacgc cgttttcaat caattctgga atatgttcga tcattgacat atccactgca
    10801 ctcatcgaga attcttcttc cacttcgcct gtatcagtta aactgtttcg ttcttgacca
    10861 aaaggcatat cgaacaatcc atacttccag cgacaagatt gagaacatcc gccacggttt
    10921 gcatcccgca tcgacatatg gttggacaat gtgcatcgac cagaataaga gatacacatg
    10981 gcaccatgga taaatgcttc gatttcggta tccgtatttt tacgaatctc tgccacttcc
    11041 tccattgaaa cttcacgagc taaaacgaca cgttccaacc cttcttcttt ccagaattcc
    11101 aatgtctcaa aattagttgc tgaagcttga gtggataaat ggatcggcag acctggggct
    11161 tctgtcgcac aaatttcgat caaagctgga tcagaaacaa tcactgcaga aataccgaca
    11221 tcacgcaaac tgcggaaaaa ttcccctgct ccatcttcat ttccttcatg ggtgaccata
    11281 ttagcagcaa cgtatacctt cgcatcatgt tcttttgcaa aagcaactcc ttcagccatt
    11341 tcttcataag taaaattacc tgcacggctt cttaaaccat atgcattccc gccaatatat
    11401 actgcatctg ctccataatg gatcgcggtt tttaactttt ccagtgttcc tgctggcgct
    11461 aggatttctg gtcgtttcaa ttttcttttt actgtcattt ctttcatcat actccttatt
    11521 tgatagcaga aggatcaata taaaagaatc ctgtatctaa ttcacggttt tcaggatgca
    11581 atttactgat ttcgctagtc attttttccg ctttttcttg atcccagcta ccttctaaaa
    11641 tttgtttttt tgcttcggcg aatagtcgag tgatgtttac gaatttttcg cctggagtaa
    11701 ataatccgtc caatttccat gtacagtaat gatgctgata aagttcattc aattcattcg
    11761 agagattcag atcattgctg gcaaaaatat gtgtgccatg actatcttca taaatagaat
    11821 aatgtgtctc ttctttttta ggttccgaga taaaaagacc tctttcacga tctttttgtt
    11881 cgtcttgttt cgtatagtta tagtaattct gtagcagtgg acgtttggat tgatggatgc
    11941 atgtagcacc atacacaagg gtttctacag gaatatcgag tttttcttcc atcgccttca
    12001 tctcttcaaa aggaacttca cgagcaagta ctgctccaac tgcgcctttt ttcgcccaga
    12061 aattgatttg acgtgcgctc gttactaatg tttcagcatc ataaatatac ggtatacgca
    12121 attcaggatt ttttttcatc gtataaataa tacctgtatc accaactgtg atcacatcta
    12181 cgtttatttc ttttaaaaaa gccaaatatt caggtacatt tttcattttt tcaggatgaa
    12241 tgatcccatt tacagcaata ttcgcttttt taccatactt gtgtgctaat tctaccaatt
    12301 gtcgctgttc ttctcgtgta aatgaagcag gcagacgcaa tccaaattgc tcttcaccga
    12361 aatatagtgt atctgcacct gcagcaagca actgttctgc ttgttcaata gtttctactg
    12421 tggttatgag ttcgatcata attttctcct cctacaactt cataatagta gcatagctct
    12481 aaaaactttc caatacttct tctatttaaa gtcggatttt tgaaaattat ttcacaaaaa
    12541 atcaaaataa agttcgatat attctgtcca aatataaaaa gagattgtga caaaaatctt
    12601 gtcacaacct cttaatcaag tcttactatt ataaaaattc agctacacag gccaagtaac
    12661 tatagggaac ctactgtttc ctttttaggt ccgcgaacat tcaatgtgat cttacctttt
    12721 gaagcaccta ctgttacgtg gtcacctaaa cgaatttctc cgccaagcaa agcctcgctc
    12781 aatcgatctt caatttcttt ttgaagtgcg cgacggattg gacgtgcacc atattcagga
    12841 tcaaatccag ctttcccgat cacatcgatt gcagatggtg tgattttcac ttgaatatct
    12901 tgttctttca atcgtttgat aatcgctttg ctcatgattt ggacgattgt atggatttca
    12961 tctttactca atgaatggaa gacgacagtc tcatcgattc gattcaagaa ttcaggtcgg
    13021 aaagctttct ttaactcttc taagatacgt ttttgcatgg ctttgtgatc tttagtaaca
    13081 tcttggacat tgaatccgac atttttttct tctcgaattt gtgtcgctcc gatatttgaa
    13141 gtcataatca tgattgtatt tctaaagtcc acttttctac ctttagaatc tgttaaatga
    13201 ccatcatcca atacttgcag aagtaaattg aaaacgtctg gatgtgcttt ctccacttca
    13261 tctaagagaa tcactgaata tggtttttga cgaatttttt ctgtcaactg tccaccttca
    13321 tcatacccta cgtatcctgg aggagacccg attaatcggc ttgtactgta tttttccata
    13381 aattctgaca tatcaacacg gatcagggca tcttcacttc caaacatgac ttctgaaagt
    13441 gctttggcca attctgtttt tccgacacca gttggtccaa ggaacatgaa tgaaccaatt
    13501 ggacgatcag gatctttcaa tccgcttcgt gcacggcgga tcgcacgtga aactgctttg
    13561 acagcctcat cttgaccaac gacacgttca tgcagaagtc cttccagttc taacaagcgt
    13621 tcgctttctt ttttctccaa ctgctgtaga gggacaccag tccactccga tacaactgta
    13681 gcaacatctt cttctgttac acgatcagaa taaccttgct cctgttttgc ttcaattgca
    13741 gaaacttttt gcagcttttt acgtagtgct ttttcttgtc tgcgtaattg agcagcattt
    13801 tcaaagtctt gtttttggat cgctgcttcc ttttcttgta ctagttcatc gatttctagt
    13861 ttgatcacag ttgattttgt taagtgatcg gtttgatcca aacggacttt cgctgctgat
    13921 tcatcgatca aatcaattgc tttatcgggt agttgacgag aagtgatata acgaacagaa
    13981 agattgactg ctgctcgtac agcttcatct gtgatctcca ctccatggtg ctcttcatat
    14041 cttgtacgta atccttgaag aatcacttct gcttcttcag gagttggctc atctacttga
    14101 atgcgagcaa aacgtcgctc taatgcagaa tctttttcga tatatttttg atattcatct
    14161 agggtcgttg ctccgatcgt ttgtaattct cctcgagcta acgccggttt caagatattc
    14221 gaagcatcaa ttgctccttc tgctccacct gcaccaatta aggtatgcaa ttcatcaatg
    14281 aataagatta cttgaccatc attatagatt tcgtcgatga cctttttcat tcgatcttca
    14341 aattcaccgc gatatttcgt accagcaacc aaagatccca tatcaagcat catcaggcgc
    14401 ttttcttgca tgtcttctgg cacttcacct aaaatgattt tttgagctaa cccttcagca
    14461 atggctgttt tgcctacacc aggttcaccc accagtacag ggttattctt tgtccgtcgg
    14521 ctaagaatct ggataagccg tttcacttct ctcgaacgac caacaactgg gtccagcttg
    14581 ttttcttttg ctaatttcgt caaatcacgt gctaatgaat ctagcgtagg tgttccttgc
    14641 ggtgtttgcc gtttatcttg tacatttaca tttctacgac gattcattcc ttttccagca
    14701 gaatcaccag ctagccccat tttttgtttc agaacttgac gggcttttga aaggctgata
    14761 cctaagttga tcatgattcg agtagccatg atactttcat cttttaataa gctcatcaaa
    14821 atatgttctg ttccaacttt ttgagaacca gttttcttca cttcgctgct agctaaagca
    14881 aaaacttgtt tcattcttgg tgaatatgga agaaagactc ctttagggta agaacggata
    14941 ccaccataac caatcagatg ttcaatttct tcataaattt ccgtttcatt caaatccatt
    15001 tcacgtaata cttttccagc aattccattt tgctcgacca ctaaggctag taaaacatgt
    15061 tctgaactaa ctgattgatg tttcaaccgt tttgcttctt cttgtgcaat ttctaatact
    15121 gcttgggctc tttcagtaaa aagttcttcc atctaatctc acttcccatc ttcataactt
    15181 aaacgttcca gaaatgctcg gagaatccga gcacgtgtat gatcttcata ttcattgaaa
    15241 ttcaatgtgt tttttgcaat tgcactcaac atcaagtttc cttctttttt tgtaattatt
    15301 tggtcttcat aaagtttttg aataatagca aaagcatttt tctcactgat tgtatcatca
    15361 aataactgat ttatctgctc aagtaattgt tttttatctg aaatccttac ttttgcaatt
    15421 cgtatataac caccaccacc acgtttgctt tctactagat aaccacgttg gatggtaaaa
    15481 cgggtattaa tcacatagtt gatttgtgaa ggaacacagt taaagaggtc tgccatttca
    15541 atccttcgaa tctcgatcat ttcactattt tccagaattt tctttatata tgattcaatt
    15601 aaatccgagg tattctgatg tgccatctta ttcattccct ttgactaata ttgaccttaa
    15661 ttataacgaa tttgataata gaaagaaaga agaacgcttt ttgttaaata aatcctttag
    15721 aatcaacaaa aaaaaatacg gaatatacta ttccgtatcg acgcgcccaa gaggagtcga
    15781 acccctaacc ttttgatccg tagtcaaaca ctctatccag ttgagctatg ggcgcatatt
    15841 caatttaaaa aaattataaa tcatttgaaa acaaaaagca agataaaaaa aatcgggaag
    15901 acaggattcg aacctgcgac cccttggtcc caaaccaagt gctctaccaa gctgagctac
    15961 ttcccgttta aaaaataaaa aagac
//
LOCUS       DANKKG010000054        15743 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000054 DANKKG010000000
VERSION     DANKKG010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15743
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.054"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(109..1077)
                     /locus_tag="PW004_001477"
     CDS             complement(109..1077)
                     /locus_tag="PW004_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HDL2726456.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            1309..2142
                     /locus_tag="PW004_001478"
     CDS             1309..2142
                     /locus_tag="PW004_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HDL2726457.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            2176..3015
                     /locus_tag="PW004_001479"
     CDS             2176..3015
                     /locus_tag="PW004_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL2726458.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            3041..3601
                     /locus_tag="PW004_001480"
     CDS             3041..3601
                     /locus_tag="PW004_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDL2726459.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            3800..5521
                     /locus_tag="PW004_001481"
     CDS             3800..5521
                     /locus_tag="PW004_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HDL2726460.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            5686..6828
                     /locus_tag="PW004_001482"
     CDS             5686..6828
                     /locus_tag="PW004_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDL2726461.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            6844..8055
                     /gene="thiI"
                     /locus_tag="PW004_001483"
     CDS             6844..8055
                     /gene="thiI"
                     /locus_tag="PW004_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HDL2726462.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            8628..9275
                     /locus_tag="PW004_001484"
     CDS             8628..9275
                     /locus_tag="PW004_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HDL2726463.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    9404..9620
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            9668..12313
                     /locus_tag="PW004_001485"
     CDS             9668..12313
                     /locus_tag="PW004_001485"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HDL2726464.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            12623..13936
                     /locus_tag="PW004_001486"
     CDS             12623..13936
                     /locus_tag="PW004_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HDL2726465.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            13926..14579
                     /locus_tag="PW004_001487"
     CDS             13926..14579
                     /locus_tag="PW004_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL2726466.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            14634..15326
                     /gene="radC"
                     /locus_tag="PW004_001488"
     CDS             14634..15326
                     /gene="radC"
                     /locus_tag="PW004_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HDL2726467.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            15476..15676
                     /locus_tag="PW004_001489"
     CDS             15476..15676
                     /locus_tag="PW004_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL2726468.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
BASE COUNT         5310 a         2699 c         3348 g         4386 t
ORIGIN      
        1 atttacgaaa attgtttctc aaatttttgt aaattttcga cttagttttc gaagaagcta
       61 tttttgtcac agcctctctt tttaagttta gctattagat aatgatgttt agttttggat
      121 aaacgaaatc aattcttcac ccgacaaacc atagtttaca gcaatcaata gttttaaacg
      181 tgctttttga ctattaatac tgttacagaa aataatcccc attttttcta gttcttttcc
      241 cccgccttca taatcataaa caggctctgc gattccattg aaacaacgag aaacaagtac
      301 tacagggatt ttttggacaa gaagcttttc caaagcagct aaggcttgtg gagggagatt
      361 ccccgcacct aaagcttcga ttaccaaacc gtctactttc gtatgggcga tggcttccaa
      421 taagtcccct tgcatcccag catatgcttt aacgatcggg atagtcccat ctacagcgga
      481 aatgtccagt cgctttgttt ctaaaagttc ctgcaagaat aaaattcgat tcttagttac
      541 taagccgatg ggacccaatg taggtgttcg gaaagtagca acatttgtgg tatgggtctt
      601 tgtcacgtag cgtgcagaat ggatctcatc gttcatgacc accaaaaccc ccttatctag
      661 cgcttcttca caacttgcga cacgaatcgc actttcaaaa ttataaaggc cgtcacttcc
      721 aagttcatta cttgaacgca ttgccccagt caaaacaatt ggtaatttgt tgccaatcgt
      781 tgtatcaaga aaaaaagcag tttcttctaa tgtatctgta ccgtgtgtga tcaccactcc
      841 ggatatacct gatgcataag cggacaagat acgttctttt aataccaaca tgtgagaagg
      901 catgatgtgt ggtgagggaa gattgaagat atcttcaact aataattcta catgcttggg
      961 gattgtgatt tctgcatcta ataatgggtt tgccacatca ggactaacac cgcccgtgag
     1021 catgttttct ttcatggcga tcgttccgcc ggtgtgtagt acaagtattt ttttcatttt
     1081 acagacttcc ttacgaataa ttttgcatag ccgttaaatc ttattgtata ctatataaaa
     1141 gagttttgct atcagtgaag aataacgaat agcctaactt atcataaaaa agctgacctt
     1201 attaaaatgg aaaagactca accttgacct cctaatacaa tggagtatct ggttgagttt
     1261 cttttacaag aaagcagcca aggaaatcaa tcacgaaggt ggccggaaat gatcagaatc
     1321 ggattaacat cgtttaaaga acatgacaaa ttaactggta aaaagaataa cactctttat
     1381 gaatatgcag ggtttctacc cttagtggaa ttagatactg cctattatgg aattccccga
     1441 aagtcctcag ttgagaattg gataagtgaa gtacctgaat catttcgctt tgtagtcaaa
     1501 gtctatcaag gaatcagcgg tcaaggagac tggaagcctt attatcagac cgaagaagag
     1561 atgattgcgc attttttaga tgttatgtcg ccaatggtag atgctggaaa gatgttttgc
     1621 tttttagtcc aattcccctc atcattcaaa tgtacgaaag aaaatgtcca gtacttgcgc
     1681 aaacttagaa agtggtttga gggttatcct gtttctatag aattgcggga ttattcttgg
     1741 tacagcgatg aatttcttga acgtacaaga aactttatgt ctagagccgc tttttcttta
     1801 gccatcatcg atgagccaca gttgctgaat acaacgattc cttttgatcc atttgtcacc
     1861 aatccgaact ttgctctttt tcgattccat ggtaggaatg cgcaaggatg gcaagcaaat
     1921 gataaagagt ggcgtaaaaa aagaacgtta tatcgttatt cagaaaaaga actgcaagaa
     1981 ctagcacatg aagtcaaagt agttgattct aagacaaaag aggtaggtgt gatttttaat
     2041 aataattctg gtggtgatgc ggctgaaaat gcattagcac tgcaaaaaat attaaatatc
     2101 aagccagatg gactcaatcc aacccaaatg gatctgtttt aagggcttat tttattcaag
     2161 aaaggggcga agtctatgat caaagctatt ttttttgata ttgacggaac attggtcacc
     2221 aatcgttcaa aagcgttgga atcaacaaaa caagcaattg aatatgctcg gaaaaacggc
     2281 attttatgcg gtgtagcaac ggggcgcagc ccagtaaaga tcaaagaaat cattgatgaa
     2341 ttggaattag atatgtatgt cgtgtataat ggtcaactgg tttttactgc agatcgcacg
     2401 attatcgacc acccttttga acaaaaagta ttggaacata ttgttgaatt tgcagatgag
     2461 aaccatcgac aaatcgtttt tggtgcgaga aatcggttag atggatcaac gacgatgcta
     2521 ttaggacaat cgatttttat caagcgacta gtcagctttt taccgcgtaa attcccggta
     2581 cgtctgatga aaaaaatcct ccaggttttt agccctcacc gacaaaagga tcgttatgaa
     2641 acgttagaga tattaaaaga accaatttat caatgtatat tgctcagtcc ggagtcagaa
     2701 cagaaaaagt tagaacatcg ctttcccgag tgtacgtttc agcgttcaaa tacgtatact
     2761 gtagatatca tcccaaaagg tggttcaaaa ttactaggca tccaagcttt cgcaaatgct
     2821 gtcgaaattg aaatggaaga aattatggca ttcggcgatc attacaatga tatcgagatg
     2881 ctgaaaggtg taggaattgg agtggccatg gggaatgctc agatcgaagt aaaacatgct
     2941 gcagactttg tgactcaatc aaatgaagaa gatgggatat acgaaggact gaaacacttc
     3001 ggtctgattt attagtcaat gaaagaagga aaaaatcagt atggaagcag agacaccttt
     3061 tcaaaaagta gaaacattcc acaaaacttt tgatccaaga agaccagaga tacccacggc
     3121 ttttacagaa aatgaagctc atacacgtgc tgcgttcaaa atagaagaac ttgttgaatt
     3181 tgttgcagct acttcaccta ataaagaaca actgcatgaa gctgttatgc tgctgcacga
     3241 agcattagat aaagcagaag aaaaaatact tggtaaaaca cagtggggaa gtgatccgct
     3301 ggttgaacaa gtagatgcac tgacagatat cctttacttt acttatggtt ctttttcatt
     3361 actaggaata gtgcctgatc gaatattcga aatcgtccat caggcaaata tggggaaatt
     3421 atttccagat ggaaaacctc attatgaccc tgtaacaaat aaagtgttga aacctgaaaa
     3481 ctggcagatg gaatatgctc cagaacctaa aatcgcggca gaaatccaac gtcagattca
     3541 agaagcgatt gctaagaaga aatccgctga tataggcatg tgcacaaatg aaaaagagtg
     3601 atcagagttt cactattttt gttatcttta tacgatggaa aaacaaaaga ttttcgttta
     3661 agttagtgaa tgcatgaaaa tagtatgcta taatctagaa tagatgtctt ttgcacgaga
     3721 gacgtattat ttggatttat gccaagcaga acgccatctg ttgcataaaa gatgcatact
     3781 gaaaacggag gaaccataaa tgggaaataa tgttatcatc ggaatcgtca ttgcaatcat
     3841 tatagtcgcc attgtcgtct ttttggttag ttacttgatg aagaaaaaaa accaagatcg
     3901 attggatgcc ttagaaaaac gcaaggaaga attatttgac ttgccagtga ttgaagaagt
     3961 cgatgatgtt aaaaaaatgc atctggttgg gcaaagccaa aatacctttc gtgaatggag
     4021 tcaaaaatgg aatgacatat ctacttcctc gttcgctgaa ctagaaagtc aaattttcga
     4081 agtggaaaac ctcaatgaaa gttttcggtt cttaaaagga aaaaaagcag ttgttgaagc
     4141 agaagctaca atggaccaaa tggagaaaca agtgacggaa atccgtgaag gtctaaagga
     4201 attgcgggaa acggaagaac gcaactcact agctgttcag caggcacttg acgtctatga
     4261 agaactgaaa gaaaaagtca gagaagacga gcatgaatac ggaccagctc tttttgaatt
     4321 gcaaaaacag atcaaagtga tcgaaaaaga atttacgcag tttgtgactt taaacacttc
     4381 tggagaccct gttgaagccc gcgaggtttt ggataaagca gaaaaacata cgtatgaagt
     4441 ggaagctctg atgaaaaaaa ttccaccgct gtatgaggag ctccatacca ctttccctga
     4501 acaacttgaa gaaatcagtg atacttacga gaaattgatg gaagaacaat atgtattccc
     4561 agaagaagat cttgctgaag atatcgcaaa agtaagtcga agaattgaaa attcattagc
     4621 caatcttgaa aaaacagaag ttgaaacggt cgaatttgaa aaccgtgaaa cagctgattt
     4681 gattgattcg ttatatgata tcttagaacg ggaaatggaa gctcagagat atgtaaaaac
     4741 taaccaatca acgattgcag agtatatcaa acatacaaca aagaataacc gtcaattatt
     4801 gattgagctg gatcacacat ctcaaagcta tacattgaac cacaatgaaa ttggccgtgt
     4861 acgaggtttc caaaccgaag tggaagaaat ggaacgtcaa aatgaacaga tgattccaca
     4921 gatccgtcaa cacgaaattc cttattcaga agtgcgcacg ttctataaaa cagtcttcaa
     4981 agtcttagaa gatatcgaaa agcaacaagt agagatcgac gactcattac atgaattgag
     5041 aaaaggcgaa aaagaagcgc aagaaaagat cgatacattc gaattcaaat tgcgcagttt
     5101 gaaacgtttt gtagaaaaac aacgtttgcc tggcttgccg aatgattacc tagaattctt
     5161 ttttgttgct actgaccgta ttgaagaact aagtgttgtc ttgaataaaa ttcgagtaaa
     5221 catggaagag gtcaatcgtt tagttgctct atgtgaagaa gatctagaat tattagataa
     5281 gaagacacat gatttagtag atgcagcagc attaacggag caaatgctcc aatatgcaaa
     5341 tcgttatcgt catacacacg aagaagtgag agaagcaatt gataaaagct actatttatt
     5401 caataaagaa tatcactatc aagaagcatt agacgaaatc ggtacagcat tagagcgagt
     5461 agaacctggt gctttcaaac gcatcgaaga tttttatttt aatcatccag acttagtata
     5521 aagcaaaaaa gttagtaaac cagcaagagc aagacggatt attgtcttgt tcttgtcttt
     5581 ttttgtatat ttgaaaattg aaacaagtga aaatttcacc tgctcttgaa agcaccttgg
     5641 acaaccgtta taatagaaaa gaatttcaaa aaagaggggt ttcttatgat atattttgat
     5701 aacagtgcaa caaccccaat ctttccgcaa agcttagatg catatgtaaa aaccagtcag
     5761 cggattattg ggaatccatc aagcttgcat gatttaggca atcaagctaa ccggttgttg
     5821 cagcaagcaa gaaagcagat cgctgatttg atccatgtag aaccagaaga aatctatttt
     5881 acttcaggcg gaacagaagg agataactgg gtattaaaag gaacgatgat cgaaaaaaga
     5941 gaatatggga atcatatgat catttcagga gtcgaacatc cggccgtcag cgagacagca
     6001 gaacaattaa aagaattagg aatcgaagta agtatcgccc cagtggataa acgcggattt
     6061 gtacgtgtcg acgaattgaa agaattaata agaaaagaga cagtattagt ctctgttatg
     6121 gctgtgaata atgaagtagg atcgattcag cccatccagg aaatcagtga tcttttggat
     6181 gattttccta aaattcattt ccatgtagat gctgttcagg cgattggaaa agtaccattc
     6241 tcgcaatggt tgacagaaag agttgatttt gcaacatttt cagctcataa attccatggc
     6301 ccacggggaa ctggtttcat ttattggaaa aagggtcgtc gtttagcacc attactgaca
     6361 ggtggagggc aagaaagaaa tcagcgaagt gggacagaaa atgttccagg tattgtggct
     6421 atggttaaag ctttacgttt atcattagag ataaaggaac aaaaacctga gcagacacaa
     6481 cgcttaaaag tagctctaat caatgcgttg aagacgtatg agaaagtgac gattttttca
     6541 gaaggaactg actatgcacc gcatatttta tgttttgctc taaaaggaat tcgcggcgaa
     6601 gttctcgtcc atgccttaga agaaaaacaa atatttgttt ctactactag tgcctgctcc
     6661 tcacgtaaac atatggcaag cagtacattg cacgcaatgc atgtaccaaa tgatctggct
     6721 acatctgctg ttcgaatcag cttagatgaa aacaattcgc ttgcagaagt cgagcaattt
     6781 atgattattt tcaagcagct ttataagaaa ttttctgcaa tcaattaaaa gaggagtgtt
     6841 cttgtgaaat acagtgaaat tatggtccgt tatggggaac tttcgacaaa aggaaagaat
     6901 cgaaaaagct ttatcatgca actagcacaa aatgtcagac aagctttgaa agactttcca
     6961 gaaatcaaaa tccatgctga tcgggaccgg atgcatcttt tattgaatgg cgcagacggg
     7021 cagctagtta ttccaaaact agcaaaaata tttgggatcc aaaatttctc tccaagtatt
     7081 cgtgtagaaa aagatgtcga agttttgaaa caagctgttc aagacatcat gaaagaaatt
     7141 tatactggga aagaaacatt taaaattatc gctaaaagaa gcgatcacca atttgaattg
     7201 acaagtaatg aattgaatca aacattaggt aatgctgttt ttgatatttt cccgcatatc
     7261 caagttcaaa tgcgtcaacc agatattcca ttacgtgtag agattcggcg tgatggcgct
     7321 tatctatcgt atgaaacgat caagggagct ggaggattgc ccgtaggaac aagcggaaaa
     7381 ggaatgctta tgctttctgg ggggattgat tctcctgtag cagggtactt tgccatgaag
     7441 cggggagtgg aaattgaagc tgttcatttt gctagtccgc cgtatacaag tgaacaagca
     7501 ctgcaaaaag caaaagatct aacagccaaa ctagcacctt atgtgggaac gatccatttt
     7561 atcgaagtgc catttaccga gattcaagaa gaaatcaaaa agtcagtgcc acaaggatat
     7621 tggatgacaa ttactcgtcg aatgatgttg cgtttgaccg atgaaattcg ccgtattcgt
     7681 catggtttag tcattttgaa tggtgaatca ttaggtcaag tggcttcaca aactttgcaa
     7741 agtatggtgg ctatcaatga agtaacgaat acgccaatca ttcggcctgt agcgacaatg
     7801 gataaacttg aaatcattga agtagcagaa cagatcgata catttaatct ttctatccag
     7861 ccttttgaag attgctgtac gatttttgct cctccacagc caaaaacacg ccctcaactg
     7921 gaaaaggtcc atcaatatga agagcgttta gcaatagatg aaatgatcca acgagcacta
     7981 gctggcttga agatggaaga gatacaagca gaatcgtcta atgaaaaaac tgaagaattt
     8041 tcagaattgc tataagaggt tatgaaaata gtgttttgac cagagtatta gagaaacaaa
     8101 acgaaaaaat agctccatgt attttaccgt ttttgaacta atgtcgaaga ttagctgatt
     8161 gaataccatt tatcaagaag ttatggaaaa gccggttctt ttttactaac tgaaaaataa
     8221 aattttattt agagatcaag ctacagttga taaatgatat aaaaaagaga atttcccatg
     8281 aaatagcgag gttgtgacag aagtgggcag cttcaagaaa taagaaggaa tttacgaaaa
     8341 ttgcttttca aatttttgtg aaatttcggc ttatttttcg aagaagttac ttctggaaca
     8401 ccgtgtatca gaggttgcga aaaagctgcc ctgcatcaag taataagaac agccaaacaa
     8461 aaatagttcc tcatatttta cgtttggttg agcttattac cgcagatgca agcttttgaa
     8521 caccgtttta tttgtctcac acccttttcc ttcctacttg taaccttgct cccgtgttag
     8581 aaacatgtta cactaaatat gtgaaattat taacaaggag cgtcgttatg cgagaaaaaa
     8641 cagatacact gccaaaagca actgcgaaaa gattaccttt atatttacgc tacttgaaga
     8701 tgctggatga ctcaggtata agtagaatca aatcaaatga gttcagcgaa atcacccaaa
     8761 ttccttctgc aacgatccgc agagacttct cgcaattagg cgagctaggc agaagcggat
     8821 atggctatga tgtccctttt cttatcgatg tattcaataa tatccttaat actaaagaag
     8881 aaaaacgaat cgcattagta ggatatggaa atcttggcaa agcattgaaa cacaacaatt
     8941 ttagaagaaa tgaaaatcta aatatcgtgt gcgtctttga taatgatcca gcactgatca
     9001 atcgagtaat tgatggagaa atgatctatc cgattgatcg tttcgcggag atagccaaag
     9061 caaaaaatgt gacagtagca atctctacgg taccaagtaa atattcacaa tctgctattg
     9121 atgaaattgt caaaggaaat gtaaccgcta tacttaattt tgcgcctgac agagtaactg
     9181 tcccagctta tgtcaatgtt caatacattg atttgacaac cgagttgcag acgcttattt
     9241 actttgatga gaattactca gaagtttttt cttaggaaaa tttgagtagg cttattgatc
     9301 ttgcgagaac tcgtcgtaat tctttcaaaa attttttagt actaaatgaa gtggtttttt
     9361 cacgaccact tgttgactaa aaaaaaaaac cattgtagaa tgaagcataa cgctgaacaa
     9421 gaggagtaac tggcacattc attcttagag agagaactat ctgctgaaag gttctcaatg
     9481 acaaccagtg aaggtagctt gggagtttct gtcttgaatg gagtagagat agacgagtcg
     9541 tgatcgttaa ctcacttgag aggcaataat gattgattat tgcaatttag gtggtaccgc
     9601 gtaaatcata cgtcctagaa tacgaaagta ttttaggacg ttttttagtt taaaggagga
     9661 aaacagcatg acggaagaaa ataacctatc aacaaaatat aatccgcaag aagttgaagc
     9721 tggacgttat cagcattggt tagaacaaga tttgtttaaa ccgagcggag ataaaaaagc
     9781 aaaaccttat tcaatcgtaa tcccgccccc aaatgttact ggaaaacttc atttagggca
     9841 tgcttgggat acgaccttgc aagatatgct gattcgtcaa aagagaatgc aaggatatga
     9901 tactttatgg ctgcctggta tggaccatgc aggaatcgcg acacaagcaa aagtagaagc
     9961 gaagcttaga gagcaaggga tttctcgtta tgatctaggt cgtgaaaagt tcatcgatca
    10021 agtgtgggaa tggaaagaag agtatgcgag ccacatccgc gaacagtggg ctaaaatggg
    10081 cttatcttta gattacagta gggaacgttt tacattagat gatggattat ctgaagcagt
    10141 acgtaaagtt tttgttacct tatacgaaaa aggtttgatt tatcgtggtg aatatatcat
    10201 caactgggat cctcaagcac gaacagctct atctgatatc gaagttattc ataaagatat
    10261 cgaaggcgcc ttttatcata tgagctatga actagcagat ggatcaggcg tagtagaaat
    10321 tgcgacaacc cgtccagaaa cgatgttggg agatacggcg attgctgttc atccagaaga
    10381 cgagcgttat caagcgctca tcggcaaaaa agtgattcta cctttagtca ataaacaaat
    10441 cccaatcatt gcagatgaat atgtagatat ggaattcggg acaggtgtag taaaaatcac
    10501 tccagcacat gatccaaacg actttgaggt aggtaatcgt catgatcttc cacgcgtaaa
    10561 tgttatggat gatgatggta cgatgaatga actagcagga aaatatgctg gaatggaacg
    10621 ttttgctgct cgtaaagcaa tcgtgcaaga tctgaaagaa atcggtcgat tgattaaaat
    10681 cgatacgatg atccacagtg tgggtcattc cgagcggaca ggtgtcgttg ttgaacctcg
    10741 gctgtctact caatggtttg taaaaatggc gccgttagcg aaaaaagcca ttgataacca
    10801 agaaacagat caagcagtgg aattcttccc tccaagattc aatcaaacgt ttttacgttg
    10861 gatggaaaat gtccatgatt gggtaatctc acgacaatta tggtggggac atcaaattcc
    10921 tgcatggtat cacaaagaaa caggtgaaat gtatgtaggg atggaagcac cagcggatag
    10981 tgaaaactgg gtacaagatc ctgatgtttt agacacttgg tttagttcag ctttgtggcc
    11041 tttctcaaca atgggctggc cagatacgga aagcgaagat tataagcgat acttccctac
    11101 tagcacttta gtaaccggtt atgacatcat tttcttctgg gtaagcagaa tgattttcca
    11161 aagcttggaa tttactggcg aacgtccatt tgagaatgtc ttgattcatg gattgattcg
    11221 tgacgaacaa gggcggaaga tgagtaaatc attaggtaat ggaatcgatc cgatggaagt
    11281 aatcgaaaag tatggagcag atgctctccg ctggttctta tcgaatggtt ctgcaccagg
    11341 acaagatgtc cgcttcagct atgaaaaaat ggatgcttct tggaacttta tcaacaaaat
    11401 ctggaatgct tcacgtttcg ttatcatgaa tatcgaagga atgaccgtag aagagatcga
    11461 tttatctgga gaaaaatcag tggctgaccg gtggatcctt actcgtttga atgaaacgat
    11521 cgaacgagta actgagctat ttgatcgttt tgaatttggt gaagcaggtc gacaactgta
    11581 taactttatc tgggatgatt tttgtgactg gtacatcgaa atgagtaaag aagtacttta
    11641 tggagatgat gctgtttcaa aacaaaccac ccgtagcatc ttagtccata cacttgatca
    11701 gatcctacgt ttattgcatc caatcatgcc attcgttaca gaagaaatat gggaacatat
    11761 tcctcatcag ggaaattctc tagtagtggc agaatatcct gttgtacgtc cagagtttga
    11821 tgatgaaaca gcttctaaag ggatggaagt attgaaagaa ttgattcgag ccgttcgaaa
    11881 tattcgttca gaagtgaata caccattgtc taaaccaatt acgttgctaa ttaaaacaaa
    11941 cgatgctaaa atcgatcaat ttttagttga aaatacaagc tatatcgaac gcttctgtaa
    12001 tcccgaagag ttgacgatat caagtgagat cactgcgcca gatctagcga tgtcagctgt
    12061 tctgacaggt gcagaaatct tcttgccgtt agcaggattg atcaatattg aagaagaaat
    12121 caaacgatta gaaaaagaac tggaaaagtg gacaagcgaa gtaaaacgtg tccaaggaaa
    12181 actttctaat gaacgttttg tttctaacgc gccggaagaa gtagtagagg cagagcgtgc
    12241 aaaagaaaaa gattatttag agaagcaaac ggctgtcaaa gaacgaatcg aaagtttgcg
    12301 tactattcaa taagaataga aaaagagagt gtgaaatcag ctagctatta ctaaaagcta
    12361 gctgattgaa catcgttaca ggaaagagtt tgagcctcac tcttagcttc gattcataag
    12421 gagatgtgac ataaattttg tcgcatctct tttttagtct actcttatta tgtaggagaa
    12481 ggagggaggt ggatcatgta tcaagagtca ataaagtagt tgataaaatc gttcctctct
    12541 ctgtttcttt tctctttgtc atacttttat ttttcttatg tgttctattt gttataataa
    12601 aagagaaatg ggggaattgt tgatgacgat tgaagaagca atagaatgga tccatagtcg
    12661 attaccattt ggttcacgtc ctggccttga ccgagtcgaa gctttattag aagtggtagg
    12721 gcatccagag agaaaagtaa aaacgatcca tatcgctgga acaaacggaa aaggttctac
    12781 tgtagcttat ttgagaagcc tattagaaga ggctgggctg acagtaggta catttacttc
    12841 accttatatc gaaacgttca atgaacggat cgcaatcaat ggcaaaagca ttccagacaa
    12901 tgatttgatc cagcttgtaa aaaaatatca gccactgatc aaacaacttg atcaatttga
    12961 agcagtagct ggtattactg aattcgagac attaacagca atggcatttg attatttttt
    13021 gatgaagaaa gtggacgtag cgattatcga agttggctta ggcggcttat tggattctac
    13081 caatgtagct gtaccaatgc tgacgggaat aacaacgatt ggacttgatc ataccgaaat
    13141 acttggagat acaattgaag aaatcgctgc acaaaaagct ggaatcatca aacaaggcgt
    13201 tccagtagta acaggaaata ttgccccaga agcttttact gtcatccaac aaacagctaa
    13261 agcaaaaaat gcaccgcttt atgtatacgg ggaaaattat aaaataaact atctgcatcc
    13321 tgatgagatg tggggagaag tatttgaatt ttttaatgaa ataggaaaga tccctcaatt
    13381 aaccactccg cttttagggc aacatcaagc tgaaaatgca ggagtagcca tcgaattgta
    13441 ttttgtttac tgtcaattgg aaaagctgcc tttccgagaa aaagatgttt tgaagggatt
    13501 gaagaaatct cgatggccag gaagaatgga gcgtttgagt aacgagccat tagttgtttt
    13561 agatggtgcc cataatacgc atgcaatgaa ccggcttgtt gagaatttga aaaaagaatt
    13621 caaaggatat caaatcaaca ttttgttttc cgccttacga actaaaaatg ttagagaaat
    13681 gctagaacga ttgcagcaag taccaaatgt ccatatttat ttgacgacct ttgaacatcc
    13741 aaaagccatc gaattaagtg aagtatcaga tttagcaagt gagagagtat caatcgtttc
    13801 attgtggcaa ttcggattag cagatatatt ggaaaagatg acaaatgaag acgtattagt
    13861 tgtgaccggt tcactttatt tcatttcaga agttcgccat ttattaatac aattaggagg
    13921 agatcatgaa gtataatgca gtgatttttg acatggatgg tttgctgttc gacacagagt
    13981 tagtgtatta cgaagccaca caagtagtag cagacaagat gggttttcct tatgataaag
    14041 acctgtattt aaaattctta ggtgtttcag atgaagaagt atgggcgaat tatcatatga
    14101 ttttctcgga atttggcaaa acaagtgtac aaagattcat agatgattca taccaggaaa
    14161 cgcttgaacg tttttcgcaa ggagcggtac aaatgaagcc cggtgtgttg gaactcctct
    14221 cttttttaga agaacatcag attcccaaag ttgtcgcttc cagtaaccaa cgagcagtca
    14281 ttgaactttt attgaagaag aacaatctgt ttgaacgatt tgagacgatt gtttctgcag
    14341 aaaatgttca acgagcaaaa ccagacccag aaattttctt gttagctaat cagaagctag
    14401 gtacagcgaa gaaagaaaca ttgattttag aagattcaca aaatggtatt ttagcagctt
    14461 ctgcggctga gatcccggta gtcatggtac cagatctgct tccaccatcc caagaattga
    14521 tcaaaaaaac agctggggtt gtttcttctt tacatgaaat ccctgcattg ttaaaataaa
    14581 aatccatctc ttttcgtatc ttattttaga taagatagaa aggagatgtt tttttgggaa
    14641 aacgattgat caaagaagta cctactagct ctttaccaag ggaacgtatg gaaatttatg
    14701 gcgcggaagc tttatcggat caggagcttt tggcaatctt actgcgtacc ggtcaacacc
    14761 cttatagtgt gatgtcgatt gctggaaatc tgttaaaaac gtttggagga ctggcctctt
    14821 tacgacaggc tactttgcat gagttagaag agattcgagg gataggacgc gtgaaagcaa
    14881 tcgaaatcaa agccttgatc gaattaggtc gacgaattca gacggatcat gaaagagcgg
    14941 ctccaccggt tcgtaccagc tacgaattgg cccagcaatt gattctggaa atgaaagatc
    15001 acaaacaaga acatttggtt tgtatttatt tggacacgaa gaatcaagtg atcttaaaaa
    15061 aaacagtgtt catcggttcg ctgaatcagt cagtcgctca tcctagagaa attttccatt
    15121 atgctgtacg ttattgtgca gcaaggatcg ttttagcgca taatcatcct tctggtaatg
    15181 ttattccatc gcagcaggat atgaatttta ccaaaaggat acaaaagtgt ggagaaatga
    15241 tggggatcac tgtactggac cacttgatca tcggacgcaa acgatatttt agtttgagag
    15301 aagaaggaat gatggaagag aagtaagttg ctttcttgca aaaaaaatga agcaagtgta
    15361 caattatatt gtaattttgt ttgggaatag aagttgaaac atctgttgca cctctatcgt
    15421 acacgagcaa tgttacaatt taaattagtg cttttggcac gaggaggata ttattatgga
    15481 aaacggaaca gtaaaatggt tcaacgctga aaaaggattt ggatttatct cacgtgaaga
    15541 tggaagcgac gtattcgttc acttctcagc tatccaaggt gacggattca aaacattaga
    15601 agaaggtcaa gcagtgactt tcgacgttga agaatcagac cgcggcccac aagctgcaaa
    15661 cgttgtaaaa gcttaatttt atctgtaaat caaattgatg gtagacatca atttttacga
    15721 gagaacaaag cgagtaacaa aaa
//
LOCUS       DANKKG010000055        15530 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000055 DANKKG010000000
VERSION     DANKKG010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.055"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            164..1027
                     /locus_tag="PW004_001490"
     CDS             164..1027
                     /locus_tag="PW004_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDL2726469.1"
                     /translation="MLMSIRFVDFGYKISHSIIGLAIVMFSLLVAPYVQLIKWSVMGM
                     LIHFILLSSILMATASDPKMGNASLYGFSYLFIVYSLPKDSLNKNFFTQTSSLLFLFF
                     CWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQEKIWMLIYAFGISLLIVAGEYVPFQR
                     LMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGSLIGCVLFIGISQFIPFNWVGILGGL
                     ALGVCSTYRYKTVFNSFGALAITASLFGVPGAVTIRIFENILGVCLGIMYIGVTEILI
                     RKIREKHGLNH"
     gene            complement(1105..1398)
                     /locus_tag="PW004_001491"
     CDS             complement(1105..1398)
                     /locus_tag="PW004_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HDL2726470.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            1566..1880
                     /locus_tag="PW004_001492"
     CDS             1566..1880
                     /locus_tag="PW004_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HDL2726471.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            1901..3880
                     /locus_tag="PW004_001493"
     CDS             1901..3880
                     /locus_tag="PW004_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDL2726472.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSGGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            3893..4219
                     /locus_tag="PW004_001494"
     CDS             3893..4219
                     /locus_tag="PW004_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HDL2726473.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            4235..4753
                     /locus_tag="PW004_001495"
     CDS             4235..4753
                     /locus_tag="PW004_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726474.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            4753..6105
                     /gene="celB"
                     /locus_tag="PW004_001496"
     CDS             4753..6105
                     /gene="celB"
                     /locus_tag="PW004_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HDL2726475.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(6189..7694)
                     /locus_tag="PW004_001497"
     CDS             complement(6189..7694)
                     /locus_tag="PW004_001497"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HDL2726476.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(7786..8376)
                     /locus_tag="PW004_001498"
     CDS             complement(7786..8376)
                     /locus_tag="PW004_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL2726477.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            8555..10201
                     /locus_tag="PW004_001499"
     CDS             8555..10201
                     /locus_tag="PW004_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDL2726478.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            10250..10969
                     /locus_tag="PW004_001500"
     CDS             10250..10969
                     /locus_tag="PW004_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDL2726479.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            11448..12395
                     /locus_tag="PW004_001501"
     CDS             11448..12395
                     /locus_tag="PW004_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2726480.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            12407..13327
                     /locus_tag="PW004_001502"
     CDS             12407..13327
                     /locus_tag="PW004_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2726481.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            13368..14825
                     /locus_tag="PW004_001503"
     CDS             13368..14825
                     /locus_tag="PW004_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2726482.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            15170..>15530
                     /locus_tag="PW004_001504"
     CDS             15170..>15530
                     /locus_tag="PW004_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HDL2726483.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATY"
BASE COUNT         4811 a         2658 c         3147 g         4914 t
ORIGIN      
        1 acaaaaaata cgagaagcag cttctaaaaa agaaaagtgc atgtatattt gttcattgtt
       61 tttacgatca ttattcatcg tattgtttgc catttgtttt atcattataa ttacaacatt
      121 atttgaatcg aagcataaac catacgctgt ggttttattc tgtatgttga tgagtattcg
      181 ttttgttgac tttggttata agatttccca ttcgatcata ggtttagcaa tcgtcatgtt
      241 ctcactatta gtagcaccgt atgtccagct gatcaaatgg tcagttatgg gcatgttgat
      301 tcattttatt ttactaagca gcatattaat ggcaaccgct agtgatccca aaatggggaa
      361 tgcttctttg tacggctttt catatctctt tattgtttat tcgttaccta aagattcgtt
      421 gaacaagaat ttctttacac aaacaagtag cttattattt ttgttttttt gttggttttc
      481 agttctcctt tacagaaaac atcgagaaaa gaacaaagac aaaagcttgt ttaaagagat
      541 ttttttgaaa ggcatgtatt cgcaggaaaa aatttggatg ctcatctatg catttggcat
      601 tagtcttttg atcgttgcag gagagtatgt cccttttcag cgattgatgt gggcagggtt
      661 tgcattttct tctattgtat ctagctatgg attgatgtcg attggtttca aagaacgagc
      721 agtagaccga atcattggct cattgattgg atgtgtttta tttattggta tcagccaatt
      781 tattcctttt aactgggtcg gtatattagg tggtctagct ttaggtgttt gttctaccta
      841 tagatataaa acagttttta attcttttgg tgctttggca atcactgcca gtttattcgg
      901 ggttccagga gcagtaacga tccgcatttt tgagaatatc cttggggtat gtttaggaat
      961 catgtatatt ggggtgactg aaatacttat ccgaaaaata agagaaaaac acggtttgaa
     1021 tcattagatg agatcgaata aaaaataagg aaaaagtgaa gatacacatg gatgataaat
     1081 tgctatcatt ccatgtgtat ctttttacgg taaatcagtg aaacgttcga ctttcatttt
     1141 taatttatac tttttacgga gttcgtttat ttgagccatc atttcggatt tgtgatgctt
     1201 ttcgattgct tcttcatctt tccaacggtc gatcagtaaa agagattctg gatcttctag
     1261 tggaaagtaa tagtcatatt tctcgtttcc ttcttctgca cgaatcgctt tgacaatgtt
     1321 tctttcgacc atttcttctg caaattttct ggcttgtcca tttgtacttg tatacaaaat
     1381 attcactgtg atactcacta aatcccctcc tgaaaaaatt ctatcactag tatatcacgg
     1441 tgccagaaat tattattcaa gatatgatac acattttttc cgcaatgcta cggaaatata
     1501 tattttgaat agaaaacgct tacaagatat gcttggttta taaaaataaa aggagtgatt
     1561 gatttatgaa aaaagcattg attatttgtg cggcaggaat gtcatcctcg atgatggcct
     1621 caaagacaac ggatttcttt aagcaaaaaa gagaagaggt actcgtagat gcagtttcag
     1681 ctacagaagg cgataatatg attaagaata gtgattttga tttatttctg atcagcccgc
     1741 aaacgacgat gtttttggat aaattcaaaa aattaggaga atctgttgga aagcctgttg
     1801 ttagtatccc tttccaagcg tatgtcccaa tccctacagg catccagaag ttagcagagg
     1861 tgatcgaaga gaacatatga tggaatagga gtggatgata atgaaacaga aggaaaaatt
     1921 gctgttgaga tatttgattg atcacaagaa ggagtatgtc accagtcaac gtttagcttc
     1981 tgaattgtta ttatctgatc ggacaatccg taattacttg cagcggatca aagagttagt
     2041 agaaaaaaac ggaggtaaga tcattgctaa accaggttat ggctaccaac tgcatatttt
     2101 acaaagactg acatttgact tgtttttgag tcggcaagaa atagtacctg gatatgctag
     2161 agaaccgcaa ggattttatg agtccgaaga tcgacaaaag tatatattga accaattgct
     2221 tttggaagat gccattcttt ttatggatga tttggcagaa gagctttata tcagtcgttc
     2281 cagtttgaca aaggacatgc aagaaatcaa ggagcgattg attccatatt cactaaaaat
     2341 cgtttctaag catggtcaag ggacatggat cgaaggggaa gaaagaaatc gacggcattt
     2401 tatcatggat actttttttg gaaaccatta tggaaattca ttgaaagaat atttagggca
     2461 aagccgtttt ttcccagaca tcagttttga agagctagtc attatcattc tcgatgaaac
     2521 aagagaagct aagttaaaag tttcggattt tatcatacag aatctgactt tacacttggc
     2581 tttggggatc aaacggctcc gagaagggtt tgaaatcaag gaattaggca tcgaacaaga
     2641 agtttctaag cgagtagagt atcaagtggc gcagcggatc gttcaacgta ttgaggtaat
     2701 cgcaggtgta tcgtttcccg aagaagaaat tgcttatttg acattacatc tcatggctaa
     2761 gtcgaatcac aaagaaaatc aaaatgatca ggaactatca aaggaattag cgaaagttct
     2821 gaaaagattg tctcaggaat ttcatttttc attagtagag gatctccagc ttttcaatgg
     2881 actactcgat catttgtcac cgatgatgat tcgattgagc ggaggtattc gcattgagaa
     2941 cccattgact gaggagataa agaaagaaaa tcccaaagtc tttgacgcag taaaaaggca
     3001 tttcagcaac atgccggctt tgaaaaacta taccatcaat gaagatgaat gggcctattt
     3061 ggctttgcat ctcatggcgg cattggaaaa agaaagagct gcgcataaac tacatgcatt
     3121 gattatctgt gcaacgggtt acggcagtgc ccaactatta aagaatcgcg tagtcagtga
     3181 attcggtaaa aatattacag tggtaagtgt aaaaggctat tatgagatca atgaaacaac
     3241 tttaaaagga atcgacctga ttatttcttc tattgatctt tcgacaatgt ttttcaaaat
     3301 tccagtttta catgtcagta tttttttgaa tgaagatgat gttcgtaaaa tacgaaaggt
     3361 agttggagaa tctataccct atcattcctc agatagaccg ataccagcat tttctctaca
     3421 gcaaaaaaag cagatttaca ccgaacagtt gtctgaaaaa tttttcaaga cttatacgta
     3481 cgaaccgaaa aaggaagaaa ttcttcatga tttgctgcaa aagctgagca taaatgaaga
     3541 agaaggatat ataagagaaa tgcgaaagca gatccagcaa cgtgaaaata tgggacaaat
     3601 catattcagt gatacagtgg cagtcccaca tcccgctgtt ccagttggaa tcgaaacaaa
     3661 ggtagccgtt gcattgattc cttctgggat gcagtgggat caatatcaag aaattcattt
     3721 tgttttttta gtttcgcctt cttatgtcga aaatgaagga attacggtcg tgactaaagc
     3781 aatcgttagg ttagttgacc agctaaaagt acagcaggaa atcttagctg aacctatttt
     3841 tgagaatttc agcagtaaat ttatcaaact gatcgaataa ggaggaaaac agatggctga
     3901 aaaaataaac agtgaaacac tacaagtgac ttcttttgat atcattttcc atagcggaaa
     3961 tgcacggaca ctgatacatg aagcatttcg ctcgatgcgt aaagagatgt ttgaagaagt
     4021 agaagagaag ttgcgacaag caaatgaaga gattcttcat gcgcatcagt cacaaactca
     4081 acttctgaaa gaatatgcca gcgggacaaa aatagaaatg gaaattatca tggtccatgc
     4141 acaagatcat ctgatgacga cgatgacatt attggaagtt gcacaggaaa tgaattattt
     4201 gtatcaaaaa aataaataga aaggaaggat cattgtgcag gatacagtaa aaaggaaaat
     4261 cgaattagaa aaaaagcaac ttagtttgaa aaacatgtat ttcaatcgtt atctaatcgt
     4321 acgttacctg actgcctttt tctttttcat gaacatgcaa tggatgattc tgcttctatc
     4381 agctaaaagt ttaggaagta gtttaccgat gattctgatt ttagctattt tgcctgcagt
     4441 aggagaacaa gtaaagcttt atcgaaaaca ccaaacgaac gttccatgga caaagtggta
     4501 ttttttgttc cagagtgtct ttaatttatt agtgattcca gtgttgttta cctcaggatt
     4561 tacattcttg tatcctttta tggcaaataa caatggagga cagttatttg ttttcattct
     4621 tgttgtaagc ggtatttttg ccagtgcatt tatacagtat cgtttaaaga aaatttcact
     4681 caaccaagac caacagtata tccgtattaa acaatatgaa aaagcgttat atttaggaaa
     4741 ggagaataac taatgtttga ttttttgcag aaatacctga tgggacctat ggggaaaatt
     4801 gcgcaactta aaatcgttcg ggctgtcatg gcagcgggga tggcttctat tcctttcacg
     4861 attgtaggct cgatgttttt ggttttaaat attttgccat taccgttccc atttttagaa
     4921 ggatttttta atgcaacttt tttcaaagtt agtgacttgt acatgatcgt caatacaatg
     4981 acgatgggga ttttatctat ttactttgcc attgtgtttg cttatgaatt aacgtcaatt
     5041 gaacgagatg aacaaaaatt aaatgttaat ccgctcacgg gggcattact atctgttttt
     5101 gcattcttta tgtgtatccc tgaattgatc atgactgatg gtaaaatcag cttgattgct
     5161 tctatcaatg agacagaaac tgtagtaaat ggtattagga tgggctcttt tgtcgaacgt
     5221 ttaggaactt caggaatctt tacagctatt attatgtcga ttattgcggt cgaattgtat
     5281 tgtatgtgtg tcaagagaaa ttgggtcata aaaatgccag atgttgttcc gccaggagtt
     5341 tctcgttctt tcactgctct gatcccaaca tttgtcattg cgtttgtggt gatgatcatc
     5401 aatggattat tagttgcact aggaacggat atcttcaaaa tgattgctat cccatttggg
     5461 tttgttacgc agttgacgaa tacatggctt ggtgtcatgg tgatttattt cttgatccat
     5521 gcattgtgga tcgtagggat ccatggagcg aatatcatca cttctttctt gaccccgatc
     5581 gtattagcta atatggcagc taacgcacaa ggcgcaaatt atccgttggc aggagaattc
     5641 aacaattctt atgtaaccgt tggtggatca ggtgcgactt taggattgat catctttatt
     5701 gcattcctcg cgaaatctga tcagctgaaa gtgttaggaa aagcctcact tgtaccagga
     5761 ttattcaata tcaatgaacc aatcattttc gggatgccaa tcgtctacaa tccctattta
     5821 gcggttccgt ttttcttagc tccgatggct tctgctacac tcgcttattt tgccattcgg
     5881 ctggaaatcg tcaaaccgat gcttgcacag atgccgtggc cttcaccagt cgggatcgga
     5941 gcatttgttg gtagtggtgg tgattggaaa gctgctgtgt tagcagtcat ttgtgcaatc
     6001 atcgcattca ttatctggtt cccatttatc aaattctatg ataataaatt attaaatgaa
     6061 gaaagagaaa aggcagcaga attagctagt gaaaagttaa aatgatttct aagaaaaaag
     6121 gaactgtgac aaaaaatcac agtccctttt tttcttagaa atgggaaacc actagtatat
     6181 aaataccatt attcttcaat caaccgcttc acgattgcat aatccccttc ataaggttca
     6241 tctacaccat tgattttttg atagggatcc acgccttttc ccgcaataac gactgaatct
     6301 tgcggagcgc tttctgctaa agcgcgtcga atcgcttcag gacgatccat ttcataatga
     6361 acgatcaaat ctggattagt aattgcttca ttgatttctt ccgcgatttt tttcggatct
     6421 tcaaatgctg ggtcatctgc cgtcaaaaag gcgatgtcag ctgtttctga tagcactttt
     6481 ccaaaatcat gtcgacggga gattgctttg tttcctgggc tgcctaatac aacaattaca
     6541 cggccatctg gatgttcatt tttggcaaat ttcagcaatg ttttcaagct tagataatta
     6601 tgtgcatagt ccacatagac atgcgctcca ttggattgga tcaattgatc cattcggcct
     6661 ggtacacgag cttcttttaa tccgtcttgt gcatcttgac tggaggcacc tgttagtgct
     6721 gcggcaatga tcgcactggc tgcattttct tggttgaatc ctccagccag caaaatatca
     6781 taagcagtat cgcagatagc taagcgatct tcatgagaag ataaagtaaa accgtgagta
     6841 cctttatctg aacggatcac ttgataattg ctttcttccg aacgaccata agtgatcgtt
     6901 ggaatctgga atagatcggc tgtttcccta agtaaatcaa aatagtcact ttcatgattt
     6961 aaaacaactg tttttgaatt caagatcaat tgtcgcttgc aatagaaata gtcatcaaat
     7021 gtcggatgtt cgatcggact gatatggtca ggcgagatat tcaagaagat tccgacatct
     7081 aaagtcaagc cgtatacgcg ttgtgttttg taagcttgtg aagaaacttc catcactaga
     7141 tgagtcatac cattttcaac tgcttctgac atcatacgat ataaatctaa tgactcaggc
     7201 gttgtcaaat gggatttgaa atatgttttg ccatctaacg ttgtattcat agtagaaagc
     7261 aatgctgttt ttttatttgt tgtgtgatcc aagattgctt ttgtaaagta cgcagcagtt
     7321 gttttacctt ttgttccagt gaatccgatc actttcaatt tattttgcgg atagtcgtaa
     7381 aaagccatag caagaagcgc cattgcttta cggatatccg ttacgatgat tcctactgct
     7441 gaattttcgt atggttgttc tgatacataa tattgaatac cctgttcgat tgctgaatcc
     7501 aaatattctt ctttgaaatt cattccttta cagaaaaaca aagtagaagc atcagctttt
     7561 cttgaatcat aacttaactg aagaaattca gctttttctg gtacatcaag atgccagcct
     7621 cttgttgaaa caaattcttt taatagattt tcttttaata gtaaatgacg gatgtcttct
     7681 aaagagaagc tcattgattg atccctcgct ttattcaatt tcttggttta ttatagcaca
     7741 ctctttttaa tatatcgttt gtttccgcta tgacaaaatc cagctttatt caaattttct
     7801 tgacacaggg gacaagcgga tgagtatttt cataaacaat gaactgactg taatagatag
     7861 gagaacgaat agtgtctcca acaatccaaa aacataataa ttcaaaaaga cgataagact
     7921 attggtaagt aaaagtcctt gcggcattgg aagccgaaaa aatttgtgga aaataatcgg
     7981 cggtatcgtt ataccgccat ttgaggcccc gactgtgtac aatgtgtaaa ccccaagact
     8041 gaacaaaaga cttcctgaaa tcagtgaaaa gaaatgtgag gagatcattt ccacttttgg
     8101 aacaatctct aaaacaacag gaaaaagcag acttcccgct acagtcttaa aaaatactga
     8161 ttttcctaat aggacagcgg ctagaagcag catcataaga ttgatgctcc aaacagtaac
     8221 tgagcgttgg atagaaaagg ccgtttcaac taaatgtccg atggcgccca ctccggcgga
     8281 agctatatcg tgagggagca agaaccaatt caatccaact gtgataaaga aaagacctaa
     8341 aaatataacg ggtagttttt ttatttcttg tgtcattgat atctcctata aatgttagcc
     8401 ataaaaggct gtatatttct ttactttacc aaaaaaacga aaccgaattc aaaaaaacaa
     8461 ttgttcttat aaaagttata taaaatcgat tgccagtttc tgttttacca attaaaatga
     8521 tagtaatgtt ttgtagaaag ttggaatctg accgatggat aatcaaagaa aaccagcatt
     8581 agatcaatta acaccgcaag aaaaaatggc acgaggctct gcttggatga ccgccagtaa
     8641 tatcatttca agattgctag gcgctgtcta tatcattccc tggtatgctt ggatgggcga
     8701 aaatgctaag gctgccaatg gattattcaa tatgggatac aatatatatg cattgtttct
     8761 actgatctct acagcaggaa tacctgctgc gattgcaaaa caaactgcac gctataattc
     8821 attgaatgaa tatggcactt cacgacgatt atttatccgt gcattacaga tgatgggcgg
     8881 gttaggcctg ttgtttgctt tgttcatgta catcgcctct ccttggctag ctcatgcttc
     8941 tggtggcgga gaagaactga ttccaacgat gcgttctttg agtatcgctg tattggtttt
     9001 tccttgtatg agtgtgatac gagggtattt gcaaggcaat caagaaatga tgccttatgc
     9061 gctgtcgcag attgtagaac aggtggcacg tattttttat atgctattgg ctacattcat
     9121 catcatgaaa gtcatggatg gtgactatgt gacggcggtc acgcaatcaa ctttcgcggc
     9181 atttatcggt atgctagcaa gtattcttgt ccttttgtat tttttgaaga aacatcaagc
     9241 gtatacatcg gcgtttattc actacagcga aaacaaaata aatattgcaa cgaaggaatt
     9301 gttgttagat accgttaaag aagcgattcc gtttattatt gtgggttcag gggtaacgat
     9361 cttcaaatta gtcgatcagt tcacttttat gaagatcatg tctgcatcca ccgagtattc
     9421 caatgcacag ttactggatc ttttttcgat ttttagtgcc aatccagata agttgacgat
     9481 ggtagttatt gcattggcaa catcgatcgc gtctactggg ttaccgttga ttacagaagc
     9541 tgttacgttg aaagatcgag taggtctggc aaaattgaca agcggaaatt tacagttgtt
     9601 ttcatttttt atgtttccgg cgacatttgg tgttatgtta cttgcctatc cgctaaatac
     9661 attattttat acaccggatt ctctaggaag taatgtactg atccaagcaa gttttgtagg
     9721 actcttccta ggactatata tgctagtttc caacatgctg caaggaatgt ttgaaaacaa
     9781 ggcggcaatt aattatttgg ttattgggtt tctagtgaaa ctccttcttc aatacccagc
     9841 cattcgttta tttgaagtat acggcccttt attggcgaca atgatcggat ttgctgtgtc
     9901 ttgtaccttg atcttaaaga gaatccatga agtagcccgc tttaaaccaa gttttgtctg
     9961 gcgcaggaca ttgctgatct ttattcttac cttgattatg cttttggctt cgtggatcac
    10021 aaagatgatt tttggtgctt ttttaaacga agatagcaaa ttccagtctc tattgttgat
    10081 tcttgtagta gcaggtatag gggctttagt gtatggttat ttagctttga aaatccgctt
    10141 ggcagaaaga ctactaggac caggaatagc gcgtttacga cataaattaa aaataaaata
    10201 agtgaatcag aagagctaga ttgatgctct tttgaaatgg gggaaacgca tgcgtttaga
    10261 taagttttta gcagatatgg gacttggcag ccgcaaagaa gtcaaaggat taatcaaaaa
    10321 aggaacgatc gaagtcaatg gacaagtggt gaaaagcgat aaagcgcaag taaatgaatt
    10381 cgaagatgcg gttacttatt taggagaaat cattacgtat caaaaagatt tttactacat
    10441 tttgcataaa cctgcaggag tcatttctgc tactcaggat aatcatgatc aaaccgtaat
    10501 cgatttattg gaagaccagg attatcggga agatcttttt ccagtcggcc gattagataa
    10561 agacacagag ggcctcttga ttttaacgaa tgacggggtg tttgcccatc agttactatc
    10621 acctaaaaaa catgtggaga aagaatatct agctgaggtt agcggtataa tgacagaaga
    10681 agatatccgt ttatttgcag aaggactgat tattgatggc gatgagcaga cattgccggc
    10741 aaaactccaa attgatcaag tagatcaaga gacagaaact tcaattatcc gtctgatttt
    10801 gcatgaagga aagtttcacc aagttaagcg aatggtcaaa gcagtaggaa aagaagtctt
    10861 atatttaaaa agaatccgta tgggaaaatt ttggctgcct aaagatttga ataaagggca
    10921 atatcgaccc atgaccaaag aagagctaga acaagtgaga gaaaaataat tgaatggata
    10981 gctaaaagaa taagcagagt agttgtagcg ccatctgaaa agcagacttg tccgtctgac
    11041 tttcggatgg cttttttgtg aaatttcggc ttatttccgt agaagctact tctggaacac
    11101 cgtttgttcg gaataaggga ctgtgacaag acttttgtta cagtccccat tgttccttgt
    11161 gactcagagt tagatagtaa ggacacgatc tccctaatac tcaggccaaa acgctgaatt
    11221 cacaacctct gataaacggt gttcaatcag ctgaccctcg gtattagctc aaaaaacggc
    11281 aaaatatgag gaactatttt cccgtttttt tctctaatac tcaggccaaa acgctgattt
    11341 cacaacctct cctatacttt gtttcatttt ccctatactt tgtttattta ttctcattat
    11401 tgaaagcgct ataattacgt tgcgaatgaa tcgaaagggg tttttagatg aaaaaacgaa
    11461 aggggttcaa tcattttttt atgaatctat ggcgttataa agcacttgtc ctgatggctg
    11521 taccgggtat gctttggatg atttttttct tttacatacc tgtaatggcg aatgtcgtag
    11581 cattcaaaaa ttttcatatt tcagcagatg ggttcttggc tagcttgagg gaaagtcctt
    11641 gggtgggttg ggaaaatttc aaatttttat tttcttcgga tcaagcattt ctcatcacta
    11701 aaaatacaat tttatataat gtagcattta tccttttgaa tctgctgatc tcagtcgtat
    11761 ttgcaatcat tatgagcgag ttgagaaata aacgactcgt taaagtttat caaacgatgt
    11821 ccttactacc ttattttcta tcttgggtca ttatcagtta ctttgtttac gcttttttaa
    11881 gtcctgacaa aggaatattc aatcagtgga tcactggaca tggaggagaa gctgtcaatt
    11941 ggtataatga accgaaatat tggccgttta tcttggtgtt cattggtaca tggaaaggga
    12001 ttggttataa cagcattatt tactttgcat ctgttatggg aatcgatccg acttattatg
    12061 aagctgctat ggttgatgga gctagcaaat ggcagcagat caaacatgtg acgataccac
    12121 aacttattcc actaatgacg attttaacga ttttagccgt aggaaacatt ttccgagctg
    12181 attttggcct gttttataac attcctagga attccggtgc attatatgat gtgactcaag
    12241 ttttggatac gtatatctac aatggcctga catccacagg agattttggt atgacggctg
    12301 ctgcaggact ttaccaatca gttgtcggct ttgtcttgtt gatgatcaca aatacgatcg
    12361 cccgacgttt tgatagcgat tctgcattat tctaaggagg aaaaaaatga aaaaaaagaa
    12421 agtggagaga gtccaagtcc gctctttcaa taagacggct aattttttct ctaatataat
    12481 gattgcactc tttgctattt cctgtatttt gccatttata tttgttatcg ccatctcttt
    12541 taccgatgag tccatattgt cgactcacgg gtacagtttt tggccagaaa agttcagtat
    12601 atttggttat acgtacttgt tcgaccagat gcaggataaa attttccaag cgcttttcgt
    12661 cactgtactt gttactgttt taggtacact gatcaatagc acggctactt ctttatatgc
    12721 ttatgcgatt tcacggtcaa actttccatt ccgtcgtttt tttaccgttt tttgtttgat
    12781 tacgatgttg ttctcaccag ggatggtggc caattatctt gttatgacaa atcttcttca
    12841 gttaaaagat acgatttggg cattgatctt gccaatggct gtcagtccct tcaatattat
    12901 tgtgatgcgc acattcttta aacgttcggt ctctgattcg attattgaat cggcacggat
    12961 cgatggagct agcgaactgc gaatatttgt tcagatcgtt ttaccgttag ctgttccagg
    13021 tattgcgaca atcagtttat ttgccgcatt gggttactgg aatgactggt tcaatgcttt
    13081 actatacatc caagatgata agctagttcc actacagtat cttttgatga aaatccaaag
    13141 taatattcaa tatctgacac aaaattcagg agcaggaagc caattgacag gcggattagc
    13201 ctcagttcca ggagaatctg ccagaatggc gatcgtagtc atctcgacgt tgcctattgc
    13261 gatcagctat ccatttttcc agaagcattt tgtcaaaggt ctaacaatcg gcggagtaaa
    13321 agaataaaga aacaaaaata taaatcataa aattaggagg aagaaaaatg aagttatgga
    13381 agaaactagc ctttacaggt gtttcagcgt tgttgttggg aacattagca gcatgcggag
    13441 gttctggttc taaagaccag gcagaagcta gcaattcgga cactttgcag atgtatcaaa
    13501 tcggagataa accagataat ttcgatcaat tgatggaagt agcaaacaaa cgaatcgaaa
    13561 aagagatcgg tgtaaaagtc aatatcaatt acattggctg gggcgattac gaaaagaaaa
    13621 tgaacgtcat catttcttcc ggggaaaatt atgatatcgc ctttgcgaac aactatgttt
    13681 ccaatgccca aaaaggagcc tttgctgatt tgactgaatt agcgcctaag tacgctgaaa
    13741 aagcttataa tgatttagat gaagcatata ttaaaggaaa tctggtcaat ggaaaattat
    13801 atgctttccc agtcaatgga aatgtctttg ctcaacaagt acttactttt aacaaagctt
    13861 tactagataa atacgatctt tcaattgatg gaatccaatc ttacgctgat gcagaaaaag
    13921 tcttgaagga attccatgaa aaagaaccaa acacggcagc ttttgccatt ggacaaggct
    13981 tcaaagtcca agggaacttt gattatcctt tagggaatac gttaccattt gctattgatc
    14041 tggatggaga tgattcgaaa atcatcaacc aatacgataa tgaaacgtat atcggactgt
    14101 tacgtacaat gcatagctgg tacaaacaag gactcattcc aagtgacgct gcaacgagca
    14161 ataaggatta tccactagaa ggaaatactt ggctaatgcg tggagaaaca caaggaccgt
    14221 atgattatgg cgatacaatt ttgacaaatg cagcaaaaca agaacttgtc tcaaaagcaa
    14281 ttactgttcc actaaaatct acagctcaag cacaaatggc aaattttgtc gtatcaaata
    14341 cttcaaaaaa taaagaaaaa tcagtagaac ttctagggct gcttaacagt gatccagaat
    14401 tattgaatgg gcttgtttgg gggatcgagg gagaagcatg ggaaaaagtc gatggttcag
    14461 atgataaaat caaattgcta gatggttatc agccaaatac acatatgtct gcttggaata
    14521 caggaaacaa caagattcta tatacacaag agtctattac ggatgacatg attgccaaac
    14581 gtgatcaatc gattgctgat gcagaaactt caccgatttt aggattcagc ttcaacactg
    14641 acagtgtcaa aacagaatta agtaatattt ctaatgtcat gaatcaatat ttagatggat
    14701 tgaatacagg gactgttgat ccagatgaaa cattaccgaa attgaaagac gctttggaca
    14761 aggctggcta tgataaggta ttgaaagaaa tgcaaaaaca atatgatgag ttccgacaag
    14821 aataataatg aataaagaca aaggggttgt gacaaaagac gtgtcacaac ctcttgttcc
    14881 gaataaacgg tgttcaaaag ccagtttctc ggtattaagc taaatatatg taaaaatgtg
    14941 gaaagccata ttcacaaaat ttttcttact actcgaaact aagcggctat ttcacaaact
    15001 cttggtaaac ggtgttcaat cagctaaccc tcggtattcg ctcacaaaac gacaaaatat
    15061 gaggaactat ttttccgttt gtttctctaa taatcaggtc aaggtgctga ttgcccaacc
    15121 tttttttgta taatgagaaa gtataaactt aatgtttgga ggagaagata tgttagggaa
    15181 ggttttggaa gagttattta tccggatctg ggtcattata aaactaacct tgtatttctg
    15241 gatatataca tttgccggcg ggatcatctt tggattaggt gcggcatgga aaacagtcaa
    15301 tgaactattt tatttgtacg gattcgagta taaagaaatc acaataaaaa gaggctggaa
    15361 tatatataaa agaaattttt taagaggcaa tctgctattt agtttatttt tatctggtac
    15421 tgctttattg agttacaatt tatatttatc tgttcaaatc aaagggctca tttttttagc
    15481 tattgatttt attttacttt ttgccttgct ctgcctattt gctacttatc
//
LOCUS       DANKKG010000056        15390 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000056 DANKKG010000000
VERSION     DANKKG010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.056"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            598..1287
                     /locus_tag="PW004_001505"
     CDS             598..1287
                     /locus_tag="PW004_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2726484.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1274..2482
                     /locus_tag="PW004_001506"
     CDS             1274..2482
                     /locus_tag="PW004_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL2726485.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2776..4047
                     /gene="serS"
                     /locus_tag="PW004_001507"
     CDS             2776..4047
                     /gene="serS"
                     /locus_tag="PW004_001507"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDL2726486.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4596..6080)
                     /gene="guaB"
                     /locus_tag="PW004_001508"
     CDS             complement(4596..6080)
                     /gene="guaB"
                     /locus_tag="PW004_001508"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HDL2726487.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6585..7280)
                     /locus_tag="PW004_001509"
     CDS             complement(6585..7280)
                     /locus_tag="PW004_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HDL2726488.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7370..8470)
                     /gene="ychF"
                     /locus_tag="PW004_001510"
     CDS             complement(7370..8470)
                     /gene="ychF"
                     /locus_tag="PW004_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HDL2726489.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8479..8670)
                     /locus_tag="PW004_001511"
     CDS             complement(8479..8670)
                     /locus_tag="PW004_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HDL2726490.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8691..9581)
                     /locus_tag="PW004_001512"
     CDS             complement(8691..9581)
                     /locus_tag="PW004_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HDL2726491.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9568..10335)
                     /locus_tag="PW004_001513"
     CDS             complement(9568..10335)
                     /locus_tag="PW004_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDL2726492.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10347..11060)
                     /gene="rsmG"
                     /locus_tag="PW004_001514"
     CDS             complement(10347..11060)
                     /gene="rsmG"
                     /locus_tag="PW004_001514"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HDL2726493.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11292..13193)
                     /gene="mnmG"
                     /locus_tag="PW004_001515"
     CDS             complement(11292..13193)
                     /gene="mnmG"
                     /locus_tag="PW004_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HDL2726494.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13210..14607)
                     /gene="mnmE"
                     /locus_tag="PW004_001516"
     CDS             complement(13210..14607)
                     /gene="mnmE"
                     /locus_tag="PW004_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HDL2726495.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14906..15289
                     /locus_tag="PW004_001517"
     CDS             14906..15289
                     /locus_tag="PW004_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726496.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
BASE COUNT         4294 a         3165 c         2850 g         5081 t
ORIGIN      
        1 ttcatcagga gtgtttcccg attcgttttt ggctgccgta gcaactttta tatcatatca
       61 gagagacaag taattgtcaa ctctttttta aaagtttttt cgatttcgtt tttcgaaatc
      121 ttccagcaac tcatttatca taacttatcg tttgttattt gtcaagaact tttttgaagt
      181 ttttttcgat agaatcaagc ttcgcaagtg gttctttttt aacgactcag ttatcttatc
      241 atgtcatttc caacatgtca acaacttatt ttttattatt taataagtta ttggtgttct
      301 tttgaacaaa taataatata tcaacatcac atgtgaaagt caataagaaa atgcttattt
      361 atcaacattt ctagcttaca tgatcattgg ttagttctgg tcctttttcg ttcgtcttta
      421 taactccgag agacgtaaaa tccttttgtt ttcagtttaa tgttcatgtt cttgtcttat
      481 tcacaccaac tagatgtctc accttctaaa attttcctta acaaagtgct gcttttatct
      541 atttctgctt ttttttgcta gaataatagt aacaatatga ctctggagga tttatttatg
      601 aaaattttga ttgctgacga tgataaagaa atcgttgaat tattaagtat ctatgttcac
      661 aatgaggggt atgaagcagt aaaggcttat gatgggaaag aagcattatc taaaatcaga
      721 acaatccctg atattgaatt acttatttta gatatcatga tgcctgagat ggatgggatg
      781 caagtagtga aagagctacg aaaagaatcc caaataccaa tcattatgct gacagcaaaa
      841 actactgata tggataagat caaaggactg gtcgctggtg cagatgatta tgtgaccaag
      901 ccatttaatc cgttagaagt aatggctaga gtaaagtctt tattacggcg tacaaaaatg
      961 cagattgccc cagatcagcc tgatgtatta gaagtaaatg cattaatcat caataaagac
     1021 tctcatgaag ttaaaacaat cgatgggaaa gaaatccagt taacagcttt ggaattcggt
     1081 atcctctact tgcttgctag tcatcctaat cgagtattta gcgcggatga aatttttgag
     1141 cgtgtttgga aacaagaaag tattgtctct gctaaaactg tcatggttca cgttagtcat
     1201 ttaagagata agattgaaga agcaactggt ggtgaaaaag tcattcaaac cgtttgggga
     1261 gtgggctata aaattgacgc aagatagtaa acgagcaact gaaaaacgcc gtcggatcaa
     1321 tttgacttca aaagaaatca gcgaattgtt agcggaagga atcatcacga ttatcctttt
     1381 gcttctatta aatgtttcta ttctggttgt tatcagttcg gtgatcaaca gttcaccttc
     1441 tttgacgaat gcgatttggg attcaaagaa tatttttgca gaacgattga acactgattt
     1501 gttctggaac ggccggaatt ttattattcc ctttttcttt ctattagata ttggtgtttt
     1561 gtattggcgc ttgattcgaa gatatcgcca aatgcaattg cggcatatca tcagcgaact
     1621 tcactacatc gcaaacggaa attatgatca tcgtatccct tttgaactga gtggtgactt
     1681 aagccgagtg gtaacaagta tcaatggatt ggtggatagt accgtagcag cgattgaaga
     1741 cgaacgtaaa atcgaaaaat caaaagatga attgatcacg aatgtcagtc atgatatccg
     1801 taccccactc acgtccatca ttggatacct aggactgata gaagatggcc agtatcactc
     1861 agaagaagat cttttaaagt atacgcatac tgcttatatc aaagcaaagc agatgaaatc
     1921 tttagtggac gacttatttg aatacactaa ggtacgccag cctgctgtcc cggtgaattt
     1981 ttctgccttt gacatgatcc agttgatcga acagttagca gctgattttg agttggaagc
     2041 ttctaaaaaa aatatccaaa tacttgttca atcaaaagta gactccctta tcatggatgg
     2101 cgataccgaa aaacttgtac gtgtcttcaa taatcttttg acgaatgcgt tgaaatacgg
     2161 aaaaggagca acgaagatcg taatcgaagt agaacgtatc ggttcagaag ttgttgcaac
     2221 agtcaaaaat aacggagcaa tgatcccaca acaggcaatc gataatctat tcgaccgttt
     2281 ttatcgtgta gaagaatctc gttcacaagc aactggagga actggacttg gtttagcaat
     2341 cgctcaaagc attgtggctt tgcacggcgg atacatttat gcaaaatcag ataagcaatg
     2401 gacttctttc atcatgcacc ttccaatcaa gaaaaatgaa aaattaccta ttgatacaca
     2461 ggaagttatt gacgaaagct gaaatattcg ctattcttat tacaattatt agaataaatg
     2521 aaaaacacag aacccagtac actttattct ttttcagaga gctgatggca ggtgtaaatc
     2581 agcaaaggat aatttggaag ttccagttcg tttacgtgcc ctttcgaaat aagaaaggtc
     2641 ggttcaacag ccgttatgtg ttcaacaagt gcagctgacg ctgaacttgg gtggtaccgc
     2701 gaatttcaga ctgattcgtc ccaagaataa tgggatggat cagtcttttt ttattttcaa
     2761 aaaggaggaa atcatatgtt agatgtaaaa atgatgcgac aaaatattga agaagtacaa
     2821 gaaaaattga tgactcgggg cgtaaaaaaa gaaacattag ctgaattcat ggaactagat
     2881 gaaaatcgtc gtaagctact agtcaaaagt gaagaattaa aaaaatatcg aaatgatgtt
     2941 tctgctgaga tcgcccaatt gaagcgtgca aaagaagatg cttctgctaa aatcgctgaa
     3001 atgaaagaag tcggtggcaa catcaaagca ttggatactg aaattgctga agtggatgaa
     3061 aaattgaaac aaattgcaac tactttgcca aaccttcccc atgattctgt gccagttgga
     3121 acagatgaag aggacaatgt agaaatccgt cgttggagcg aaccacgtac atttgctttt
     3181 gaaccaaaac cacactggga aattgcagaa aatcttgata ttttagattt tgaacgtggc
     3241 gcaaaagtag ccggtagccg ttttgtctat tacaaaggat taggtgctcg attagaacgt
     3301 gctgtctaca acttcatgct tgatcagcat atttacgaac acggctatac tgaaatgatc
     3361 actccttata tcgtcaacag taaagcgatg tttggtacgg gacaatttcc aaaattcaaa
     3421 gaagatgttt tccaattgga gaatactgat ctaacattaa tcccaacagc tgaagtacca
     3481 ttaactaact attataatga tgaaatttta gatggtgcag atttacctat ttatttcact
     3541 gcactaagcc ctgcttttcg ttctgaagcc ggaagtgctg gtcgggatac acgaggactg
     3601 attcgtcttc accaattcaa taaagtagaa atggtcaaat tttcggatgt agagcattct
     3661 tacgaagaat tggaaaaaat gacagctgat gcagaagata tcttgaaaaa attgcatttg
     3721 ccttatcgtg tgatggcctt atcaactgga gacatgggct tttctgcagc taaaacttat
     3781 gacttagaag tatggatacc tgcacaagat acttatcgtg aaatcagttc ttgttctaac
     3841 tgtgaagatt tccaagcacg ccgcgcaatg atccgttatc gcgataatga tggaaaaatc
     3901 cactatactc atactctgaa tggttcagga cttgcagttg gccgtacagt agctgccatt
     3961 ttggaaaact accaaaatga agatggttct gtcacagttc cagaagcgct cgttccttat
     4021 atggggaatc tgaaagttat taaataaaga ggctgtgaaa aagccgctta gtttcgagta
     4081 ttaagaaaaa ttttgtgaaa atggctttcc acatttttgc ataaattgga cttaataccg
     4141 agaaactggc ttttgaacac catttatcga gaggttgtga aatcagcgaa ttaacctgag
     4201 tattagagaa aaaaatggaa aaatagtccc tcatatttta ccgtttgttg aactaatacc
     4261 gagggtcagc tgattgaaca ccgtttagtg gagaggtcgt aagcctaccc cccagctcct
     4321 agttacaagg acaaattcaa aaaacagctc ttcatatttt atgaatgttg tacttgtgat
     4381 tcggaggtgg caagggaaca ctgtttattc aaaaagaggc tgggacagaa gcgtttaact
     4441 ccaagaaata agaagaaatt cacgaaaatt gcttttcaaa tttttgtgaa tttcttctta
     4501 tttccgaagg agttgcttct acttccgccg tttatacgtg tttagagcgt gagacaaaag
     4561 tgttctttac ttttgtccca cgctctttaa tgtttttaag attcaactga atagtttggt
     4621 gcttcttttg tgatttgtac atcatgaggg tgtgattctt ttaatccact gccgctcatt
     4681 tggataaatt gcgcttcatc acgtaattgc tgtagattgg ctgccccaac ataacccatt
     4741 cctgaacgca aaccgccgat catttggaaa ataatatctg caacacttcc tttataagca
     4801 acccgacctt cgattccttc tggtactaat ttatttgctt cattgacact accttggaag
     4861 taacggtctt ttgaaccttt ttccatcgcg cctaatgagc ccatacctcg gtatgttttg
     4921 aatcgacgtc cttggaagat ttcgaattca cctggtgatt catctgtccc tgccaacata
     4981 cttcctagca tcactgcatg tccgccggca gctaatgctt tgacgatgtc accagaatat
     5041 ttgatccctc cgtctgcaat aatcgctttg ccatattgac gagcaactga tgctgcatca
     5101 taaatagctg tgagctgagg tacacctaca ccggctacca cacgtgtcgt acaaatcgat
     5161 cctggtccga taccaacttt tacgacatct acacctgcat catataaagc ttttgtcgct
     5221 tcagccgtcg caacgtttcc tgcaatcaat gttgcatctg caaaagttga acggatttct
     5281 tgaatttttc ggatcacacc tgcactatgt ccatgagctg tatcgatgat gattgcatct
     5341 gcccctgctt ctaataaagc attcgcacgc tcaaatgtat cgcttgttac accaacggct
     5401 gctgctacaa gcaatcgacc atgttcatct tttgctgcat ttggaaattc gatcactttc
     5461 tcgatgtctt tgatcgtgat caatccgctt aaacgacctt cgttatctac gattggtaat
     5521 ttttcgattt tatgtttttg caaaatcttt tctgcatctt ttagagaggt acctactggg
     5581 gcagtcacta agttgtcttt tgtcatcaca tcagcaatcg gcatgtgata atctgttaca
     5641 aaacgcatgt cgcggtttgt aattatccct accaattttc tgttttcaag tgtttcaacg
     5701 attggcacac cactgatacg atatttgctc atcaattctt ctgcgtctgc tacaagatgc
     5761 tgcggcgtta agaagaacgg atcaatgatt acgccacttt cagaacgttt gacttttctt
     5821 acttcatccg cttgctggct gatcgtcatg tttttatgga tcacacctag tccgccttgt
     5881 cttgccatag caatggccat cttgctgtcg gtaacagtat ccatacttgc actgatgatt
     5941 ggaatattca acttaatatt tttagctaat tgtacgctca tatccacttc gtttggtaac
     6001 acgtgacttt ctgctggtat caataagaca tcatcaaaag taaatccttt tttcgtgaac
     6061 tttgtttccc agttggacat ttttagtacc actcctctaa tttagtttat ctattaaaat
     6121 aattgatttt ttcatttaag tcaatgatct acttggaatc aagttagctt tctcatagaa
     6181 taccaatata aagcgtttac atctgtgtgt attaaaagag attgtaaaaa agctgtcctg
     6241 catcaagtaa taagaacagc caatcaaaaa cagcttctca tgtttttgaa ttggttgggt
     6301 ttattaccgc agatgcaaac ttttgaacac cgtttagtgg agaggctgtg aaaaagctgc
     6361 ttagtttcga gtagtaagaa aaattttgtg aaaatggctt tccacatttt tgcataaatt
     6421 ggacttaata ccgagaaact ggcttttgaa caccgtttat cgtaaaaagg gactgtgaca
     6481 agacttttgt cacagtccct tactattatt taactatgga taaacaaaat gaggttcttt
     6541 actaccccta gatatctagc aaaatagctg atctttttgc tagtttatct tcttgggacg
     6601 gataaacttc caataatccc gtattttttc ttcaagtagt aacggattgc taaagcagtt
     6661 gccccgataa tgataactac tactggatct aatactggat tgataactgg tggcaatagt
     6721 gttgtcgcag aaaacagtac gacccaaaga agtgtcgttc cacctaggat aagcaatgtc
     6781 ttacccattc ctggtttctt gcttcgatct gctccgggat attcatattg atagatgtat
     6841 ttgtacatca tataaaagac ccagccaccg attgcagaac ctagaacgag tgtagaaaag
     6901 ccataagctg gcgttgttcc acgtgaaaac aatccggtaa tcgcaatcat gatacttaat
     6961 agaccaagca acagcaacgt attatctaac cacatcaata aaggggtgga tgaatttttt
     7021 acttctgctg gcttattgat gattgcttcc gttctttcag agactgtccc aaacaattga
     7081 cgggctgttc ttccgccttt ttgttctgca actaaagtag gcagaatgtc atgaagggct
     7141 tttacttttt cttcttcaga caaattcgct gcttctaaag attttttcaa atcaaaaatg
     7201 tactgttgat ttcttttggt taattttgtt tctaaactat tgttttcttt tacgattgct
     7261 cgtaattgtt ctggttccat tatctaatag tcctccttag ccgttccttt tctcaacggt
     7321 cgtcacaaaa gacattctac catattctac agctagtttg atagcccttt tacacattaa
     7381 agcggaataa cattacgtcg ccgtcttgta cgacataatc ttttccttct aatcgaacac
     7441 gtccagcttc ttttgctgca tgcatgtttc cgtaatgatc caagtcttca aaagagacag
     7501 tttctgcacg gataaatccg cgttcgaaat ctgaatggat gattcctgct gcttgtggtg
     7561 ctttgattcc ttttttgaat gtccaagcac gtacttcttg ttccccagca gtaaagtaag
     7621 tagctaagcc taacaaatca taagcagcac ggatcaactg atccaaacct gattcttcga
     7681 ttcctaaagc ttctaaaaat tccgctttgt cttcttcatc taattctgcg atttcttctt
     7741 ctgcacgagc actgaccacg atcacttctg cattttcttc tgcagcaaag ttgcgcactt
     7801 cttgtacata atgattcgcg tctgcatctg ctacgtcatc ttctgagaca ttggctacat
     7861 aaagaattgg ttttgtcgtc aataaaaata gtgatttaac gattttttgt tcttcttcag
     7921 taaattcgat cgttcgggca gattttcctt cttccagtac aggtttgatt ttatccagta
     7981 cagctagttc tgccactgca tctttatctt tggttttagc aacttttgct acacgagtat
     8041 agcgtttgtt gatagattct aagtctgcta acactagttc caagttgatc gtatcaatat
     8101 cttctagtgg atcaacacgt ccttctacgt gagtaatatt atcatcatca aaacagcgaa
     8161 caacgtgaca aatagcgtct acttggcgga tatggcttaa gaattgattt cccaaacctt
     8221 ctcctttact tgcgcctttt acgattccag caatatctgt aaattcaaaa gtcgtcggaa
     8281 cagttttttt cggatgtaca agttctgtta agcgttgcaa acgccagtca ggtacttcta
     8341 ccatccctac gttggggtcg atcgtcgcaa agggatagtt tgctgcttct gctcctgctt
     8401 ttgtgatcgc attaaacaaa gttgattttc ctacgttggg caacccaaca attccagcag
     8461 tcaatgccat aaatttattc actctttctc tgcatttttt tcaattactt tttttaattt
     8521 cttttcaaaa tctcgacggg tcatcatgac gatatgtccg caattcatac atctgatttt
     8581 gatatctgcg cccattcgaa tgacttccca gcgattcgtc tggcaagcgt gaggtttttt
     8641 catttcaacg atatcgtgca agtcgtacat aaaaatagcc tcctttttgt ttattcttca
     8701 tcaaattgaa tattcaagat atcaagaatt cgagtcaaat ctttttgaga aagatattca
     8761 atctcgatct tacctttccc atttttttct tggatagcta cacttgtacc aaatttatcc
     8821 atcaaacgat cttcgctttc tcggatataa tatggttttt cctttgctag ccttgggatt
     8881 tttttctttt cttttccatc attttgattt aactcagaaa caagctgttc cagttggcgg
     8941 acagttaaat tttcagttac tgcacgattg gctaatttca agatcaatgt tttatttttt
     9001 aaacctaaca gcgtacgggc ttgtcccata gataaacgct gatcttgtac catttccttg
     9061 acaagatccg gtaaagaaag caagcgcaaa taattggcga tataaggtct gctctttcct
     9121 aaacgactag ctacttctgc ttgtgttagt ttcaggtttt tcatcagcat ctcatatgct
     9181 tctgcttctt ctaacggatt cagatcttct cgctgcaagt tttccagcac tgctacttgc
     9241 atcatcgctt cttcgtcaaa gtctctaacg atagcaggga tcgtttcttt ttcagctagt
     9301 ttggacgctc gaaaacgtcg ctcccctgca ataatctcat atccttttac tgcggaacgg
     9361 cgtacgatga tgggttgaaa gacacctgat tgttcaatag aattagctag ttcctgcaaa
     9421 gatacttctt caaatgtttt acggggctga tacggatttg gtcgcagctc attcaatggt
     9481 agctggacaa cggtatcatc ttttacatcc accgtctcta agttttctaa atcttgaaac
     9541 agtgcatcga ttcctcggcc taatccttta cttttcttca cgacttacca cttcctttgc
     9601 caatgcttgg tagacttcag ctcctcgaga acgtggatcg tagtcgatga tcggttttcc
     9661 atgactaggt gcttctgata aacgaatatt tcttggaatg atcgtttcgt aaactttttc
     9721 gcggaagtat ttacgtactt cttctacgac ttcatttccc agatttgttc gtgcatcata
     9781 cattgtcagt aagactccct cgatctctaa ttctggatta aagtgttttt ggactaaacg
     9841 aaccgtattc agtaattggc ttaatccttc caatgcgtaa tactcacact gaacaggtat
     9901 caaaatcgaa tcacttgctg taaatgaatt gattgtcaga tggccaagag aaggcgggca
     9961 atcgatcaaa atataatcat actgactgct tacttctgct aaagatcctt ttaatcttga
    10021 ttctcttgcc atcattgacg tcaattcgat ctctgctcct gctaattgga gagtcgcagg
    10081 cacaatactc aaattttcgt gttcagtgat caaagttgcc tcatcaattg gcaattcatt
    10141 gactagaaca tcatagatgt ctcttgtcac atcaggtttt ctgatcccga caccactggt
    10201 agcatttcct tgtgcatcca tatccactag caatactttt ttgccaaggc tagcaagaca
    10261 agcacctaga ttcaccgtcg tcgttgtttt gccgacgcca cctttttggt tcgcaacaga
    10321 tattatttgt gccatctctt ctcctcctat ttgattggct gcttattagg cagtccgggc
    10381 tttcttgggt atttctttgg ggttggtttc tttttttgaa taacgatgat atgccgttga
    10441 tcagagacat tcggcaaggt aaccgcctct tctttgagga atttaccacc taataaagca
    10501 atcgcttgtt ttgcttctgt caattcctct tcactttttg ctgctttcaa tgcataaaaa
    10561 tagccttctt gtttagccaa gggcaaacac aattccgcta acacattcaa acgagcaacc
    10621 gcacgagcag tgacaaggtc aaatgatgca cgaaagtgag gattttgccc aaatgtttct
    10681 gcccggtcat gatataagga agcatcgatt cctaataatc gggttaactc agataagaag
    10741 gttattcgtt tattcagaga atcaacgatc gttatctgca gttctggaaa ggcaattttt
    10801 aaaggtatgc taggaaaccc tgcccccgaa ccaacatcgc ataaagataa aggttgcgta
    10861 aagtctactt caaatgctaa tgtaagtgaa tcgtaaaaat gcttcagata tacgtctttt
    10921 tcatccgtaa tagctgtaag gttcatcttt tcgttccatt cgactaatgt ccgataataa
    10981 atagcgaatt gattcatttg atgatcggta agctctatcc ctttttctgc caaggattgg
    11041 cgaaattctt ctggtatcat aaatattcct ttcattgtga aacagcaatt gtttcacaga
    11101 ttctctccct actttaacgc aaaatctcta gacatgcaat ccttccatat ggattcttaa
    11161 aaatttgaaa agtaagaaaa gccgatagac tttttctttc tagtatgact cataaaatat
    11221 acacagaaga gagaactagc tttgagcaat ttctgcaaaa actagttctc tcttctattt
    11281 attattacgt attacgctgg ttcactgact ttcgcaatct tcccttgttc gatatagacc
    11341 attaggatac ttaggtctgc agggtttacg ccactgattc ggctagcttg ggcaattgtt
    11401 tctggttgga ttttctgcaa tttttgtttt gcttctgttg ctaatccgtt gattgctgcg
    11461 tagtcgatgt tttctggaat ctttttcgct tccatgcgtt ttaatttttc tactttttcc
    11521 atggcttttt tgatgtagcc ttcatatttg atttggattt ctacttgttc gatttctttt
    11581 cgtgtcaatt cttcattttc tggaataaac cgtagaagtt ctgcataagt aatctcagga
    11641 cgtcttaaaa agtcgcttgc tagtacacca tcttttaatg gtgccgcatt aatcgttgct
    11701 aaaaagtctt ggacttcttc tgttggtttg attcgtacat tcatcaaacg tttgatttct
    11761 tgttcaactg cttctttttt cttcaaataa ttcttgtatt gatcttcttt tactaagcct
    11821 acacggtgac ccatttctgt taatcgcaaa tctgcattat catgacgtaa aagcagacgg
    11881 tattcagcac gtgaagtcag caaacgataa ggttcgttcg ttccttttgt taccaaatca
    11941 tcgatcatga caccgatgta accgtcactt cgtttgatca ctaaaggttc ttttccctga
    12001 actttcaaag ctgcattgat accggcaatc agcccttgtc cagccgcttc ttcataaccg
    12061 cttgtaccat ttgtttgtcc agctgtataa agattttcaa cgactttagt ttctaatgtc
    12121 ggacggagct ggtgtggctc tactacgtca tattcgatag catatcctgt acgcatcatt
    12181 tctacttttt ctaatccagc aatagaatga agcatgttga cttgaacatc ctctggtaaa
    12241 gaagtagata aaccttgaac atagacttct tccgtattta agccttctgg ttccaaaaat
    12301 aattgatgac gtggtttgtc tgcaaaacgc acgatcttat cttcgatgga tggacaatag
    12361 cgagcgccta ccccttcaac gatccctgta aacattgggg cacgatgtaa gttgtctcga
    12421 ataatttggt gagtcgtctc acctgtatag gtcagccagc aagattcctg atctagttta
    12481 taggcactat ctggtgtgct gaagctaaaa tgattcggct ctttgtcacc aggctgctct
    12541 tctgtaacag aatagtcgat cgtacttgat tttactcgag gcggtgttcc tgttttgaac
    12601 cgttcgatag caaagccaag ttcttttaga tgattcgcta gtccaataga aggttgagag
    12661 ttattcggac ctgatgaata tttcaattca ccgataatga tttctccgcg aagtgctgtt
    12721 ccggctgtaa taacaactgc tttggcccgg tagctcgctc ctgtggacgt cactactcca
    12781 tgacaaacac catcttcgac gatcagttct tcgacgattc cttgtcttag cgtcaaattt
    12841 ggttcttttt cgatcgtatg tttcatttct gttgaataag catgtttgtc tgcttgtgct
    12901 cgcaatgctc ggacagctgg gccttttcct gtgttcaaca tacgcatttg gatataggtt
    12961 ttatcgatat tcttacccat ttctccgcct aacgcatcga tttctcgaac gacaacgcct
    13021 ttagctggtc cgcctacaga aggattacat ggcatgaatg ctaccatgtc caaatttatt
    13081 gtcagtaata atgtttttga tcccatacgt gcagcagcaa gtgcagcttc tgaaccggcg
    13141 tgtcctgcgc caacaacgat cacgtcatac gcatctgctt cataatgttc caatcgtttg
    13201 ttcctccttt tattttccta aacaaaattg actgaataat tgagtgatta gctcatcttg
    13261 tacactgtcg ccgacaactt ctcctagata gtcccagcaa cgcgtcatat ctatttgaac
    13321 gagatccaca ggcattcctg aatcaatacc tgcaatgact tcttcaagtg agagggaagc
    13381 tttttctaac aacgcgatat ggcgggtatt agaaacataa gtagcgtctc tttctcctgt
    13441 ttctccgctg aagaataaat cagaaatagc tgattccaaa cgatctagtc cgtcgttttt
    13501 agctacggaa attgaaaaaa ctggttcatt ttcgatcaat ttcttcaatt tttcttgttc
    13561 cagctgtgct ggcaaatctg ttttatttag aagaatgatc cgtttcaatc cacttgtcgc
    13621 ttccaataat tgttcatctt ctgctgtcag tggttcgctt tggtttagaa caagtaagat
    13681 caagtctgct tctgctaatg cttttcggct tctttcgacc ccgatccgtt ccaccacatc
    13741 ttctgtttcc cgaattccag ctgtatcgat tagcttgagc ggtacccccc gaacattgac
    13801 atactcttcg attacatctc gagtcgttcc tgcaatatcc gtaacgattg ctttttcttc
    13861 tcttaacaaa tggtttaaca aacttgattt gccgacattt ggccggccga tgattgccgt
    13921 acttaaccct tcccgcagaa ccttcccttg cttggatgta gcaagtagcg cttggattct
    13981 ttgttggatc atctgcgctt tttcaagcaa taacttcgta gtcaattctt cgacatcatc
    14041 atattctgga taatcaatat ttacttctac ttgtgctaat gtctctaaaa tctcttgtct
    14101 caatgagcgg atcaaagcag acagattgcc gtctaactga ttaagagcta gtcccattgc
    14161 tttgtctgtt tttgcccgaa tcaaatccat cacagcttct gcctgtgaaa ggtcaacacg
    14221 accattcaag aatgctcgtt ttgtgaattc gcctggctca gctaatcttg caccttctct
    14281 taacaatagc tgcaaaattt gattgactac aacgatccca ccgtgacaat tgatttctac
    14341 tacatcttcc cgcgtaaatg tctttggtgc tctcatcacg gagaccatga cttcatcgac
    14401 aagctgattg ctcttgggat caacgatatg tccataatgg atcgtatgac ttggtacttc
    14461 tgataatcgt ttgtttccag attgataaac tttatctgct agttgcacag cttgatcgcc
    14521 acttaaacga acgatactga ttgcaccttc tccaggtggg gtagaaattg ccgcaatcgt
    14581 atcaaattct aaagtaattt cagccatttt ttcacttcct ttttcatttt gcgcacaaaa
    14641 aaagtgccca ctccaccctt tatgcacaat aaacggtgaa cttcgcactt tgactgcttg
    14701 ttcttgatta aattctggtc atagattatc acacttgcta taaggaatca agtgtttaaa
    14761 acaattagat gaagttgagc ctgttctgct tccattacct tatatttaat tgtccttgga
    14821 tgtctctttg cgttctttgg tttattcgaa gagaagatac tgtataaaca aggctttatc
    14881 tttcttaaaa aaaacggtac actaaatggt agcgctatct aaactataca cttttgaaag
    14941 gagaaaatat gtgagcccgt tcagatctgc tgcattggaa gaagaaagtg accctcggat
    15001 caacgatgtc gttttatcta cagggaatat caaccagaaa tatatcgtcc gcgatatcgt
    15061 ttttgttgtg gaatgcttag aagctgattt gtttgaccca tcttttccca aagatgacct
    15121 tttcttaaca acgaagagga aattaaagac gaaggtactt gaatatggtg cgaatgcagt
    15181 aatcaactgt cattttgagt atcagcaaaa aaaacgcgac ggaaagtatt ttctggaact
    15241 ttttgcttat ggaacagtga tccaatttat ccaaacaacg attggttaaa tgaaacagac
    15301 aaaaaaagcg gatcaaaact gaagacagtt tcgatccgct tttttggctc tatacttttt
    15361 gatgttggtg aacttcacca acatcttttt
//
LOCUS       DANKKG010000057        14116 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000057 DANKKG010000000
VERSION     DANKKG010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14116
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.057"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..729
                     /locus_tag="PW004_001518"
     CDS             <1..729
                     /locus_tag="PW004_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726497.1"
                     /translation="AALFTFTAYTQLTVADSQESTDSTEQTIQTSDSSAIQSSDETVD
                     SSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGQKLEKQEGVNGAKFSVYDVS
                     DILQKMDVKDLTTDQIESQLKDRVKKLSSDQLKLISNGETKTIDQQTGVFEFSVEVQA
                     NQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSEASQPKEEVKKI
                     VSTGVKKNFFENCWDFVTHLFSRD"
     gene            742..1002
                     /locus_tag="PW004_001519"
     CDS             742..1002
                     /locus_tag="PW004_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726498.1"
                     /translation="MKLIKLLLFNLSCLTAVFLYCGLLFSQLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            999..3089
                     /locus_tag="PW004_001520"
     CDS             999..3089
                     /locus_tag="PW004_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HDL2726499.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYTHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDNMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVDYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEVSAQVIDKSKGKDITSEGTLTYDSNLRTLKWEASADFLSNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTISAVSLLL
                     LVLATFSYAVLRYI"
     gene            3116..3580
                     /locus_tag="PW004_001521"
     CDS             3116..3580
                     /locus_tag="PW004_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726500.1"
                     /translation="MKKLIIVVTSLIAMIVILVGCSNEKNKQTNQDNGVLKSGTMWKE
                     EVGGLVYNLKIIDETTWEYSESVWHPDPVQITVKRQKDYKGLERYKIVDSAGVREFIN
                     KSDSLFIVVPYEKNGVKKIIFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            3577..3819
                     /locus_tag="PW004_001522"
     CDS             3577..3819
                     /locus_tag="PW004_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HDL2726501.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            3837..4139
                     /locus_tag="PW004_001523"
     CDS             3837..4139
                     /locus_tag="PW004_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726502.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAAAA
                     VASIVIGGLFFLFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGLFGQTPQGL"
     gene            4209..6269
                     /locus_tag="PW004_001524"
     CDS             4209..6269
                     /locus_tag="PW004_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726503.1"
                     /translation="MYFTIAGNTKTDKELIKKATKEVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGKVLEHESRSSVKLTLTLHDTEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVNQGSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEESTWTEGDSQEYIEPLISEEVEKNEIEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVA
                     KINNFTQTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLELKKREQD
                     LAEQQMAIEKQRLNQEKEKEAARKERQEQMDTLRQAQLNYLAQPQPTPSVAAVQTPMN
                     NQTNLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQFSQADSVATTASIKEEA
                     QEQARKEYEQQLEELLNRTNQSTTESSMENSKESDPSKAKEGSSDSTVQSNTSESSES
                     NEVK"
     gene            6269..8878
                     /locus_tag="PW004_001525"
     CDS             6269..8878
                     /locus_tag="PW004_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HDL2726504.1"
                     /translation="MRVRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVKIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAILMGLKMDMWFRPLADGQKGDNRLKTLKEIQQIYPQ
                     IPESKGGFPGYGGIPITHYKKYWYIQTDTVNTCIIGTSRSGKGQIEVLATIDNLSRAE
                     RQSSMVVNDPKTELYVASKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYQDPENDYKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNIL
                     STAITGLNPFTLTKIAKMTSQSTIDYKSIGFPKYLDLKLHESLLNQRIVVEFYHQGKK
                     FHEERVKVGYRGFCEINFDCHLEQGDHVCLKYDHQGKEYVAWYEFSQRVLKDEEGNIV
                     YRKKREETHLPELEKEAVLTLDEDKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHII
                     VSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNMLW
                     DLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENST
                     TNLLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNHG
                     ETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGLH
                     DQLEEKNEENMFYKIVQSLENETAQKLLIQFYEEQATDRLQEYVEKLIKNEEISEMIG
                     KRLLLEAEKAVKE"
     gene            8875..11406
                     /locus_tag="PW004_001526"
     CDS             8875..11406
                     /locus_tag="PW004_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726505.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PKQAKEMADKDKSGLSDLQKDRLRALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEEKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKEND
                     QSFVHQSTAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            11456..11788
                     /locus_tag="PW004_001527"
     CDS             11456..11788
                     /locus_tag="PW004_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726506.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            11789..12382
                     /locus_tag="PW004_001528"
     CDS             11789..12382
                     /locus_tag="PW004_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726507.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            12545..>14116
                     /locus_tag="PW004_001529"
     CDS             12545..>14116
                     /locus_tag="PW004_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDL2726508.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTETQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQR"
BASE COUNT         5216 a         2250 c         2847 g         3803 t
ORIGIN      
        1 gccgctcttt ttactttcac tgcctacaca cagttgacgg ttgcagacag tcaagaaagc
       61 acggatagca cagaacaaac aatccaaacg tcagatagca gcgcgattca aagcagcgat
      121 gaaaccgtcg attcgtcaac agttgattct tcagaagaac ctaaaattgc tgaagacgat
      181 caaatagtca aacagaccat tcacgttcaa aaaattattt cttccgaaca attagacaac
      241 caggggcaaa agcttgaaaa acaagaagga gtaaatggcg cgaagttttc tgtttatgat
      301 gtatcggaca ttttacaaaa gatggatgtt aaggatctca ctactgacca aattgaatcc
      361 caattaaaag atcgagtgaa aaaactatcc agtgatcaac tgaagctaat ttctaatgga
      421 gaaacaaaga caattgatca acaaacaggt gtctttgaat tctcggtaga agtacaggca
      481 aatcagaaac aagcttatta tattgtcaat gaatcaagtc ctgaaaacat ttctaatagc
      541 gaggatattt tgctattaac acctgtttct gataaaaatg gtgaattctt aaaggatgtg
      601 tggatttatc ctaaatcaga agcttcgcaa cctaaagaag aagtaaaaaa aatcgtatct
      661 actggcgtaa agaaaaactt ctttgagaat tgttgggatt ttgttactca cttgttttct
      721 agagattaag gaagaaataa gatgaaactg atcaaattat tattattcaa tctttcttgt
      781 ttaaccgcag tctttttgta ctgcggttta ttattttctc aactatctat tttgccaagc
      841 taccaactat acctaatgag gattttaaac ataccagctg cttccatgcc gctagatatt
      901 atggaaaagc tcattaggaa tccattattt actacgatca atttgatcgt tattgtcgga
      961 atttcactag tcatttataa aaaagagggg atgaagggat gaattttaaa cactcgtgga
     1021 caagacggtt aaaatatgtt tttcccataa tgatgatgtt aggtacattg tttgggttaa
     1081 aaacagccaa tgtctttgct gaaaaagtta ttgtcgatcc gtcaaatcca atccagaacg
     1141 taaagaataa ttttattatt ccaaaagatg ttcctaatgc aaaaacaaat attactgtaa
     1201 acggagcgtc aaaagtttac aaatatcatg ttgatgccaa caaaggtggt tttacagacg
     1261 actacgtagg attagtggag ggaaacaaaa atatacgcta tccttctttc caaaaggaat
     1321 acggtgaaac aaaccttgtt ttagaaggcg taacaacaaa caccaaagtc aatattgact
     1381 atggaaaaat cggtacgtat aacgggaaaa aagtcaatat caagctagtt ttatctaata
     1441 ttcacttata ctcggatacg ttgccgtgga atctactaga taataactat acaaaaactc
     1501 attttcgaga cgatggctat aaaaatacga atggtgcaat gagtaaatct aaaaaaagaa
     1561 cagtactttg gatctcagac aatcttttct caggaattgt ttaccattca acacaaatga
     1621 atgtgcagct agtagccaca tatgaggacg gtagccccgt tcaattttca ggagatacat
     1681 ttatcagttt taactctttg aaccctgcag gaggaaaaag cacggatctt aaaggagaat
     1741 atacccacta tgacaaaatg aatacaacag attggtatgt cagaaaagat acggttcttt
     1801 cagaatttaa atctttctac aacaatctga acgtggtagg tggccaccca ggaggaagct
     1861 ctaagctgac acaagcggat aatgatttca ataatttgca tgataaatta ggagatccga
     1921 agtttggtca aggtactgtg tcctttaaaa tatctgaagc aaatccgacg tttgtgattg
     1981 gttcttctaa tgttcaaacc tggtttactt tatcttctgc gacaatcttt agtgttgttc
     2041 ctgatcaacc tgaaaaaaca ggggttgata aaaatggaaa taacgtgaac gataacatgt
     2101 tgcaagtagg agacacgatt caatatcgaa tcaaacagaa ggtgaatcga ttgggagtgg
     2161 atttactagc ggtttacgat cgatttgagt taatcgataa tttaccaaaa gaagtagatt
     2221 acgtcgatgc acacgttgaa agtggtacaa ataaaaaatt cgatgtatct ggtgaagtga
     2281 cctatgacaa aacaaaacac caggtaaaat atgcagcgaa agctgataca ttaaaaaatc
     2341 gaatgaagta taacggcgaa acttacgaat tagtgatcaa tgtaaaagtc aatgaattgg
     2401 ccaatcaaaa tagtgtagcc aaaaatcagg gaacgtcgat cattaataaa gtagaaaagg
     2461 atacaaatat cattaccgtt tatttcccta aaattccagt gaaagaagta caacaaaatg
     2521 gaaaagatgt aaacggacga aatgacggta agaagggtac gccgacagct cctttaaacg
     2581 caggatctga agtacaatat ctagtcacac aaaaatggca cacaaaaggc gtagacgctg
     2641 cgtcagatca ctacaaacaa tttagtattc aagatccaat tgaagctcgt ttgacatata
     2701 aagaagtttc tgcgcaagta atagataaga gtaaaggaaa agatattact tctgaaggca
     2761 cactaaccta tgatagcaat ttaagaacgc tgaagtggga agcttccgct gacttcttaa
     2821 gtaacaatct tttagatgga cgagaaattc agctgatatt tacagctaaa actccattac
     2881 aatcagaaaa aaatattgat aaccaagccg tagtagcagt agataatgtt tctaataaaa
     2941 caaatgttgt gacaattggg gttgatccta acttgccaca ggtcattgtt cctaaaacag
     3001 gttctacaca tttagtaacg atttcagcgg tcagcttact attacttgtg ttagcaactt
     3061 ttagttatgc ggttctgaga tacatttaaa ataaaaaaac gaaagaatga ataaaatgaa
     3121 aaagttaata atagtagtta cttccttgat tgccatgatt gttattttag tcggatgtag
     3181 taatgaaaaa aataaacaaa ctaatcaaga taatggcgta ttgaaatcag gaactatgtg
     3241 gaaagaagaa gtaggtggtc ttgtttataa tttgaaaatt atagacgaga ctacctggga
     3301 atactctgag agtgtatggc atccagatcc tgtacaaata acagtcaaga ggcaaaaaga
     3361 ctataaaggt ttagaaagat acaaaatagt agattcagct ggtgttaggg aatttataaa
     3421 caaatctgat tctttattta ttgttgttcc ctatgagaag aatggcgtaa aaaaaataat
     3481 ttttcttgga tctagcaagg atgaaaagcg aacgaaagag aaattgattc acgatggatc
     3541 acaatccaat aaatataagt tacaaaagac tagcgagtga ttgctagtct ttttatttgt
     3601 cctgaaggag gaatcacagt ggcaaaagct aaaacgccat ttgaaaaagc cgcgtctaaa
     3661 atcttagaac gccgcggtgt atctgaaaca cagtggaaac aagaagtcct tgaaaaagca
     3721 caatttgacc tattacgggg aaaagacaag gacttagaag gatatatcta tcaacgtgaa
     3781 cgtgaaaagc ttgttttcga tgaaattatt aaacactaaa aggagcgaac aattttatgt
     3841 ttgaatcaat tacaatgaat tttttaacac aaccgaaagt agtagctgcg gttgatattt
     3901 ttagagcatt acaaactgcc gcaactagtt taaaaaacaa tctgatgatt tttgctgctg
     3961 ctgcggccgt tgcttccatt gttattgggg gtcttttctt cctatttgga cgaagaggag
     4021 ctgaaaatgg gaagatgcac gtaggctatg tattgatggg ggtaggagtt attttagcag
     4081 cagcttctat tgtggtttcg ttatttggat tgtttggaca aacacctcaa ggattataag
     4141 aaagtaaaaa agtaggagta agaagtagtc gatacttttt actccttttt tatgaaagga
     4201 gaatactcat gtactttacg attgcaggca ataccaaaac agataaagag ttaataaaaa
     4261 aagcaacgaa ggaagtagga aagcgtactt cttttttacc gaaaacaatt aaatattctg
     4321 tcattgaaac ggaagaacag ctacatgtgt tagggaaagt actggaacac gaaagtcgat
     4381 cttctgttaa gttgactctc acgttacatg acacagaagg aaaaatagag aaagctggta
     4441 cagaatacgt ttttggccca ctggattatc aaatttcaga aggtttctat cctacattga
     4501 tggatttaat ccgccttgat gtgtttaaag aaacggatga aactatgagt gtggaagagt
     4561 cttatcgaaa cgaaaaactt ttacaagttt tagaagaaga gttagagagc gaccaaagaa
     4621 aagtgaatca agggagtgaa gtcgaagtaa atgaagaagt accatgggaa cgaccaatat
     4681 ttccaaagaa ggagtcaaaa gaaaaagaga cagtctatgt tgacgaacaa gctgatattc
     4741 cgactcagaa atcgatattt gaggaagaga acgatgtgat gggtcaattg gaggtagatc
     4801 cctttgaaga aacaatggag gatgaagaga cggccgaagt agaagttgat ccagttgaag
     4861 ctaatgaaga gtcaacatgg actgaagggg attctcagga atatattgaa ccacttatca
     4921 gtgaagaagt agaaaaaaac gaaatagaga tacaaaaggc aactgtattt gattatattt
     4981 tggatcaata tccgccagag catgaatgga tgaaagaaaa aaacaaatta tttttaaatg
     5041 agctttattc gaaatatcaa ttaccccaaa cctatcaagc atttttagag agtaaagaag
     5101 aattaatctc acagggtaaa ggcacacttt tagaacagtt agagtctgtc caatcaacaa
     5161 attggcatga gcttgcacaa gaagatttat tagccacctt tgaagaacgt caacaagaaa
     5221 cagatcaaga aattaatgag tatgcgacgg aacagtcgtc taattgggag aaagcaaaag
     5281 aggaactaaa taaagaagaa cagcgagtga ttgaagaaga agtacaaaaa attaaagagc
     5341 gatacgaaaa gaaacgcgac aatgcgtatt ccttgtgtgt agcaaagatc aataatttta
     5401 ctcagacgaa tcaagaagcg ttatcgaaag aaaaagaaaa cttgttaaaa gaacgtatta
     5461 aagaacgaaa agaagaattt taccgaggtt tgcgtacaga taagctaaag attagcaaag
     5521 aattaaatga acaactgaat aagctatatc aaacaacgac tgaagttctt atggaaaaac
     5581 acgaacagat ccagcaagaa ttacaagtac aaattccaaa atggaaaaaa gagtatgaaa
     5641 ttcaacaaca agaattgaaa gaacaagaag aaaaacaacg gcaaaaagaa aaagagcgcg
     5701 aacaattaga actaaaaaaa cgtgaacagg atcttgcgga acaacaaatg gcaatagaaa
     5761 aacaacggtt aaaccaagaa aaggaaaaag aagctgctcg taaggaacgt caagaacaaa
     5821 tggatacatt gcgacaagct cagttaaatt atctagcaca acctcaacca acgccttcag
     5881 tagctgctgt tcaaacccca atgaacaacc aaacaaatct aactccttca ccacagcgtt
     5941 cctttaacgg atggatggtg ggatgtattg caagtttaag cttgcttcta ggtggaggga
     6001 cagtatttgc ttttaatcat atcaatcaat tttcacaggc tgattctgta gccacaacgg
     6061 cgtcaatcaa ggaagaagcc caagaacaag caagaaaaga gtatgaacaa caattggaag
     6121 aattattgaa tcgtaccaat cagtcaacaa cagagtcaag tatggaaaat agcaaagaga
     6181 gtgatcctag taaggctaaa gaaggatcaa gcgattcaac tgtacaatca aacacttccg
     6241 aaagttcaga atcaaatgag gtgaaataat gcgtgttaga gaaacaacga gacgaaaaga
     6301 caaacgattc aatttagaaa agaataggta tctttctcat ttctatcgct atactccttc
     6361 aggagaagag ctaccagaaa aacatgacaa acgtatttct caaaagattt tttatagtgg
     6421 attagcttat cttcccttaa actatgttgt tacaagaata ccagaagcat ttcatcgatt
     6481 tgagattatt ccaggaagtt tacaaggagt gaaaattttt cagttacaag aaatgccgca
     6541 gttattccat tttgattcaa ctggtttttg gaatcctttg accccaacaa caagtttagg
     6601 cacacgttta gttgtttcta cgatttgtgt tgttccagct atacttatgg gattgaaaat
     6661 ggacatgtgg tttcgtcctc tagcagatgg gcaaaaagga gataaccgat taaaaacgtt
     6721 aaaggaaatc caacaaattt atccgcaaat accagaatca aagggaggtt ttcctggtta
     6781 tggcggaata ccaattactc attataaaaa gtactggtat attcagacgg atacagtaaa
     6841 tacttgtatt attggtactt cccgttcagg gaaaggacag atagaagttt tagcaaccat
     6901 tgacaatttg tctcgtgcag aaagacaaag ttcaatggtt gttaatgatc caaaaacaga
     6961 attatatgtt gctagtaaag atgaactaga agctagaggg tatgatgtgt atgcatttaa
     7021 tatcaccgat ccactgcaat caatgagtga taatccattg gcactcatcg tgaaatactg
     7081 gaagcgagga gacatagata ctgcaacgca gttaaccaat acttttacga atacgattta
     7141 ttatgatgcc aatgcttcag acaacaaata ctttaacgaa aatgcgcaga aagctgtaaa
     7201 tgcgcttatt tttgcgttgt tagaacaagc cgatcaaaca ggggattact ccaaattaac
     7261 acccaataat cttgttgagc ttctttctga aattggcggt tttaattatc aagatcctga
     7321 gaatgattac aaacaactca atgccttaga tgaattcatg aatcagttac cacctggaaa
     7381 catagcaaaa aaacaatatg gggcaacaaa aattggttct gataaagcaa aagggaatat
     7441 tttatcgacg gcaatcacag gattgaatcc atttacccta acgaaaattg cgaaaatgac
     7501 ttctcaatca accattgatt ataagtccat tggttttccg aagtatttgg atctcaaact
     7561 acacgagagt ctgttaaatc agcgaattgt tgtagagttt tatcatcaag ggaaaaaatt
     7621 tcatgaggaa cgtgtcaaag taggttatag aggtttttgt gaaatcaact ttgattgtca
     7681 tttagagcag ggagatcatg tttgcttaaa atacgatcat caaggaaaag aatatgttgc
     7741 ttggtatgag ttttctcaaa gagtattgaa agacgaagag ggaaatatcg tttatcgaaa
     7801 aaaacgagaa gaaacccatt taccagaatt agaaaaagaa gctgttctaa cattagatga
     7861 ggataaaagt aacttattag tagagtctat tcggatgcac tataccgata aaccaatggc
     7921 tatttttatg ctcacacctg attatgatcc atctaaccat atcattgtta gtatttttct
     7981 tagtcagttg tataaagaat tatctacgca atgtgttaag actaaaggag ataaatgtca
     8041 ccgtaggatt cattttattt tagatgaatt tgggaatatg ccaccaatgg ataatatgga
     8101 aggaatcatg acagtcacag ctggtagaaa catgttgtgg gatctgttta ttcaatccta
     8161 tcagcaactt tatgcgaagt atggggaaca aaacgcagcg attatcaaag aaaattgtca
     8221 gacacatatt tatattatgt cgacagacga taataccatc gaagaatttt ccaaaaaagt
     8281 ggggaataag acggttgaac aagagaacag taccaccaat ttattaggta tgaatactca
     8341 tatcaatcgc aatgttgatt cagatcgtat cttaactcct gaaagaattt ctactttttt
     8401 agaaggtgaa acgattgtac tcgctccttt aaaacgacaa gatttgaaag gacataaaat
     8461 acggccgtat cctattttta atcatgggga aacgaatctt ccttatgctt atcaatatat
     8521 tgaagatttc aatccatcgt gggatatcaa tgatatagac atacattgta cacatgcgga
     8581 cttggatctt aaggctttac aaattgatta tgattatttc ctaccagatg aatatgctag
     8641 aaatctctat catggactgc atgatcaatt agaagaaaaa aacgaagaaa atatgtttta
     8701 taaaatcgtt caatctcttg aaaatgagac agcccaaaaa ttattaattc aattttacga
     8761 agaacaagca acggatcgtc ttcaagaata tgttgaaaag ttgatcaaaa atgaagaaat
     8821 ttctgaaatg attgggaaac ggttgttatt ggaagctgaa aaggcggtga aagaatgatc
     8881 attggaggaa ttttttctgg catagccgac tggtggtcag gcttattcgg agaaggggat
     8941 gcaaacccca aaaccgtaac gaaagtctta caagattatg gtaattatct gcagtatcaa
     9001 gatgtattta gttggatttg gaacactttt ctgtggggat tgattaaagg attatataat
     9061 ctcaatactt tactagaaaa aaccatttat caaagctttg atttaaaaga cttattgaac
     9121 gccgctgggg taaatgcact atttcaaagt ttgattagta aggtattggc tcttttttgt
     9181 gtcgtcacat taatttattt aggcttgaaa ttttttatga gtaaacaccc accaaaagtc
     9241 aaaaatatct tggttaattt atttatggcc atgatcttga tttttggtgg gagttcttta
     9301 atcgatgaag ggttaaatat cagtaaaagc ttttacggag acgtcactgc agcaaataaa
     9361 gcagataaaa cgggttcacc tgcttttcaa attattcaaa ataatgtgat cgatgtcagt
     9421 acattactag caactgaccc gaataaagta gaatccctgc caacagacaa aagaaatgct
     9481 ttaacagcga aaaattttaa aacagcgaat atcaatggga ttattactcc taaacaagct
     9541 aaagaaatgg cggataaaga taagagtggt ttatcagatt tacaaaagga tcggttaaga
     9601 gcgttacaat accgattgga actagatgat aatgggaaag aaattcctgt tcaaattagt
     9661 gacgaaggct tagcaaaata tgtctatact tcagggtatc gtcgatacag tagtaaacca
     9721 ggtattatct tagctggtga atcttctttg gcggtggcct atttatttat cctatttacg
     9781 atcgttactt gcattattga gctggtattt aaaaaattct atttagtgat tgccgcgtca
     9841 acggactttg agacaggcca acggatgaaa agtgcgatag aagatatttc tcaatccttc
     9901 ttgttattgg cgtttacgat tttagaatta cgaatctata cattaatgct ttcaggtatc
     9961 ggtgatttgc atgcggcagg aaagatcaat ggattcttgt atgttgttgc tttaattgtg
    10021 ctaactattg cgctattcaa aggctctcaa tcggttacga agattttcgg tgttgatacg
    10081 agtctgaaaa acggcagtaa ttctcttctt agcatgtttg ccttaggaaa tattgcgaaa
    10141 aatgtgggta gcggagctaa aaaccttgca agtgctggta agagtggttt aagccattta
    10201 aatgatattc gtaaaaatgg atttggccat cgaaaagatg ttacccctga taacggggaa
    10261 gaacaatcag acgataaagc tacaactgta cctaatagta agaaaacagt ttccgaaatg
    10321 ttcgggaaag cgaaaaatgg tgtttctaaa gccgcagaag gtcttggtta cgtcaatgaa
    10381 cgtgggttaa aaggaacagc tgaagatctt acagaaaaag ccaaagacac cttagaagag
    10441 aaattagacg gcacgagagc taaagaattg gctgaagctg tgaagaatcc atccgaagca
    10501 ctagaaaaac aaaaagaaaa agcttcagaa gtcaaagaag gactaaaaga tgcgtatggt
    10561 gaaggacaag ttaatgcagc agtaaaagct aatacaacaa ccgaaaaaga aaatcaagaa
    10621 ggtatagtga ttccttcagt caaagatcac caagaagcgt tgcactctaa tgaaaattcg
    10681 attgaagaga aaaattccat agaagaaaga aattcagaag aaggaaaaaa tggacagtta
    10741 ccaaaaactg atccagaaca atatcgctat aaacctaaca gtgaccttga aaatgagaag
    10801 cgaatttcag atttatcaaa agtaaatcaa gaagacaaag cagaaaatga gggacagtta
    10861 ccaaaaactg atccagaaca atatcggtat agatctgtta actccgaaaa tgaaaaacgc
    10921 atttcagatt taccaaaacg aaatcaagaa gtacaaacgg atgaggattc catcgttctg
    10981 ccaaatgata atggaatcga agttggaact gaaaaatcaa cgaaaattgg tttacctaag
    11041 gcacaaaatt taccaatgaa agatagtcgt ttgaatcaat tcaaggagaa tgatcaaagc
    11101 tttgttcacc aatcaactgc aggtactgtt caaatgaaac caagtcgttt gcctgaaaat
    11161 ggaaatctga caactgtttc ttacaattta tctgaaagtg gtgagacagg gaatgcgtcc
    11221 gttcaaaatg ttgtgacaag agaagctgca ccaaaaccta ttcaaaacgt tcatacaggt
    11281 tacaagccag caaatgtggc atttactgca ccaacaaaaa taaatgtgac aagagaaact
    11341 aaaaacagga acaatgcatt gaaagaacaa ctattgaggg atatcaaaca ggatatcaaa
    11401 ccataaaagg cctacaagtt aggcctttta tttatatgaa aaggagaaat caattatgga
    11461 taaatatata aacatgaaaa accctccaag atttagtgga gaatggaagt ttttcgcttt
    11521 tttcttttta gatctattaa cgcttggtgg ctgctttttt tgggcgaaaa tggtcaataa
    11581 tcttgtccac ttaccactct tttttgcact cttgaattat ggcttatgga ttgcgatagc
    11641 gttagtgatt gtttggcgac cctataataa cccaggtcaa cgacaaattt ttgcgattat
    11701 cgagtctatt ttttttgcgg acaagaacca ttatcatgcg ttagatccta accgaaatta
    11761 tcatggtacg tataaggagg atgaataaat gcgagtcaaa ggacaaaatt cagttgaaag
    11821 gaaatccatt ttgcagtttg gaaaaccaga agagcgctct atcttggact tatttccgta
    11881 taaatcctgt acagaagagg gagtcttaat tactagagaa gatcattttc aacgttatta
    11941 tcgtgtagtt tctacagacg tggaaggatt aaatgaacaa gagaaactag aacgaatgaa
    12001 tcagttgacc accgtcatgc gtacgtatgt gccaacaatt aagttagtta gtttgaccac
    12061 agagacggat ctaacggaac aaatcgtgca aaagcggcaa ttattagata aaaatcgtat
    12121 ggaacagtcc ttaggaagaa atctacaacg attaaaaaaa tatgaaaaga aattagtcga
    12181 agaaatccag gaattgaaac gagcagaaca agaacgtcct gacttaagct tctttttcgt
    12241 cattgaagga aagacaatca aggatttaca aaccagaaac cgtcagttaa tgcgatctag
    12301 tggcatttta gggttaaaac cactctacaa aaaagaattg atcaagattc tgtatcgaat
    12361 gaacaatatg aatgatgagt aggaatggag aagagagaat gaaatatccg ttgatatggt
    12421 tagaaaaaga tgttccgaag gaagagacca ctagaaaatt tcctcacact ttaagcctgg
    12481 gtaagttggg aaaagggcaa tcttttaacc aagtaaagaa tcaaacaaga agaggtgact
    12541 gtacatgtta gaagcaatta aaacagcttt ttcaccacaa aatgatcaaa aagtacttga
    12601 tccattaaag caactaggaa atcgggaaat agaaatttta aaatccaaag ggtatgattt
    12661 agatttttta aaacaagtga tgccacaagg tgggttaaat tttgatgaag aatatgtgac
    12721 gtatggtaat ggttattcac gatgtgtgac agtttatcac tacacaaaaa atcctacgct
    12781 tttttggttg gccaatctca tgaacaatcc tcatacagtg gccacactcg atgttagcac
    12841 agatgaaaag caaaaagtga ttgatcaaat caatcgtgcc ttagatgaat taaatgatcg
    12901 atcagataat gaacgaaaag cgactgaccg aaataaaagc gcagaagaat acctggaact
    12961 agcggaatat gctcgtaaat tgacttctaa tggggaaatt tctaagcaag tcaaagtgag
    13021 agtacatgtt tacggaaaca cgattcaaga agtagatgaa cgagcgcagg aactgatcga
    13081 aaatctaaaa gggatggatt atcgtgcggt gacttatctg cataaaacca aacgagattg
    13141 gcaatcgatg tttaccatgt tcgatgaaca agctgaatgg ttcggaaata tcaaagggca
    13201 atcactacca tcgttaaaca ttggcggagg tttcccgttc caccaccaaa cgctaaaaga
    13261 tccaggaggg acacaatatg gggaaaccat gacaggcggc ccatttgtct ggaatgcgac
    13321 gtataaaaat agtgtacggt tgtctactaa taccttgctt ctaggaatga tgggagctgg
    13381 taaatcgacc gttcttaaaa tgatcgcgga agcccattta gctgcaggag actttatttg
    13441 gggctttgaa aagggaaaag actttattcc tttcttaaag gaatataacg gtattatggt
    13501 gcgtttagac ggatctgacg gaatgatcaa tcctttagag atatttgcca cacggacgta
    13561 tgatgaagca tccagtttat atgacgatgg atcagtcaaa gatttgaaga tcaatgaagc
    13621 agcgtcctat caaacacact tagacaaagt ggtctatcag gtacagttag tgagtcctca
    13681 gctaaaagga acgatgaaag cagaatttaa gacctactta aatcgttttt atgaagagta
    13741 tggaacagta ccacgcggct ttacttcctc aaattcgcgt tcaaatacgg agacgcaagt
    13801 cacaggtaaa gatccagaag cttatccgac gttcaaagaa tttttagact ttttaggaca
    13861 actggaatta ccaggtgctt cccaagagaa aaagaatcga aaagaagaaa tggaatccat
    13921 cgtagaatcc ttatgtgaga cgtatggcat gatttttgat gggcatagta cgattcgaca
    13981 ccttgaccaa caacagttgg tcttttttga cattgactct attacaggct tagatcgagg
    14041 ggtacaacaa tgtcaaatgt acatggctat gacgttgatt tggaatcagg cattgattca
    14101 aggaagaaga caacga
//
LOCUS       DANKKG010000058        13959 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000058 DANKKG010000000
VERSION     DANKKG010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.058"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..135
                     /locus_tag="PW004_001530"
     CDS             <1..135
                     /locus_tag="PW004_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL2726509.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
     gene            complement(313..2310)
                     /gene="tkt"
                     /locus_tag="PW004_001531"
     CDS             complement(313..2310)
                     /gene="tkt"
                     /locus_tag="PW004_001531"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDL2726510.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2412..2663)
                     /locus_tag="PW004_001532"
     CDS             complement(2412..2663)
                     /locus_tag="PW004_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HDL2726511.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2810..3433
                     /gene="lexA"
                     /locus_tag="PW004_001533"
     CDS             2810..3433
                     /gene="lexA"
                     /locus_tag="PW004_001533"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HDL2726512.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3613..3831
                     /locus_tag="PW004_001534"
     CDS             3613..3831
                     /locus_tag="PW004_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726513.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(4120..4632)
                     /locus_tag="PW004_001535"
     CDS             complement(4120..4632)
                     /locus_tag="PW004_001535"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HDL2726514.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4646..6943)
                     /gene="recJ"
                     /locus_tag="PW004_001536"
     CDS             complement(4646..6943)
                     /gene="recJ"
                     /locus_tag="PW004_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HDL2726515.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(7085..7528)
                     /locus_tag="PW004_001537"
     CDS             complement(7085..7528)
                     /locus_tag="PW004_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HDL2726516.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7529..8317)
                     /locus_tag="PW004_001538"
     CDS             complement(7529..8317)
                     /locus_tag="PW004_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL2726517.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8314..9255)
                     /locus_tag="PW004_001539"
     CDS             complement(8314..9255)
                     /locus_tag="PW004_001539"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HDL2726518.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9432..10019)
                     /locus_tag="PW004_001540"
     CDS             complement(9432..10019)
                     /locus_tag="PW004_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HDL2726519.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10240..11547)
                     /gene="obgE"
                     /locus_tag="PW004_001541"
     CDS             complement(10240..11547)
                     /gene="obgE"
                     /locus_tag="PW004_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HDL2726520.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11841..12851
                     /gene="gap"
                     /locus_tag="PW004_001542"
     CDS             11841..12851
                     /gene="gap"
                     /locus_tag="PW004_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDL2726521.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12936..13871)
                     /locus_tag="PW004_001543"
     CDS             complement(12936..13871)
                     /locus_tag="PW004_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL2726522.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3863 a         3126 c         2454 g         4516 t
ORIGIN      
        1 ttcaaccaag tcaatcaagg attcatctca tccgttgcgt ccaaaagaaa ccatatccct
       61 agaaaatcac taaattacca aacaccatta gaagtttttt tgagttacgt aaatggaaag
      121 ttttgtctcg cttaatttga caaataatat tctcaagaaa cacaaactaa tccgaagaat
      181 tctttgtttt gtcatttaaa tgtaacatag ccgaaaacga aaagtaatat tgatttattc
      241 ttctaacaca taaagaacag ctgtctccaa cttgcagaca gctgttctta tcagtcaaac
      301 aattgcttgg atctatgata gttgattgta cgtattcacg acattttcta ctgtaaatcc
      361 aaattcttct aaaattttag tgccaggggc agatgcaccg aaatgatcga ttgtaatcgt
      421 tgcgccctct gttcctacgt agcgttccca tccaaatgat gcagcagctt cgatagccac
      481 acgtttcttc actgatttag gaagtacact ttctttatat tcagaagatt gtttttcaaa
      541 gaggtcaaag cttggcatag aaacaacaga aacatctttg cctttttcag ccaattcttt
      601 ttgagctttc acagccaaat caacttcaga acctgttgca atcaaaatac cttctggtgt
      661 ttctccttgt gatggagaaa gaacatacgc gccttttttc accatttcat ctgctacttc
      721 ttttgtagaa ggcaatactg gaaggttttg acgactcaat acaaggatag taggcgcatc
      781 tgttgtctcc atcgctactt tccaagcagc acgtgtttca tttccgtctg ccggacgaat
      841 cacttgaaca ccaggcatgc agcgaacaga tgctagttgt tcaatcggtt cgtgcgttgg
      901 tccgtcttct ccgacagcaa ccgagtcatg tgttaagacg taaacaacag gagtatgttg
      961 gattgctgct aaacgaactg ctggtcttag ataatcgaca aagacaaaga atgttccacc
     1021 gtagatacga gttccgccat gaagttggat accattcata gccgaagcca ttgcaaattc
     1081 gcggacaccg aaccagatgt tgcgtccttc ataatgttca ggagtgaaat ctttatctgc
     1141 agccaccatt gtattattgg aacctgacaa gtccgctgaa ccaccccaga agcttggtat
     1201 agctttcgat aattcttgga tgacttcttt acttgaaaca cggcttgctt gactgcttcc
     1261 aacttcataa gttggcaatt ctgcatccca gttttctggt agtttgccat caaaagcatc
     1321 ttcaaattgt tgtgctaatt caggataagc ttttttataa ttggcaaaca tttcattcca
     1381 tgcatcttca gctttttgtc cttcttctat catcgtttga tggaagcggg cagctacttc
     1441 ttctggaaca gtgaaatctg gatactccca accataaact tctttagcca tcttgatgcc
     1501 atcttcacca agaggagcac catgaacagc agatgttcct tctttaggag caccatagcc
     1561 gatgactgtt tttacttcga tcaatgttgg tttgtctgtt tcagcttttg cttcttcaat
     1621 cgctttagag attgcttcta ggtcatttcc gtctttcact aagatatgtt gccagccata
     1681 tgcttcatag cgagcaccaa cgttttctgt gaaagctttt gaagttggac cgtctagtga
     1741 aatatcgttt gaatcgtata aaacgatcaa tttccctagt ttcatatgac ctgccatcga
     1801 acttgcttct tgagataccc cttccatcaa gtctccgtca ccgcatattg catacgtata
     1861 gtgatccatc acattaaagt tttctttgtt atacgttgct gcaaggtgcg cttctgccat
     1921 tgccataccg acagccattg caatcccttg tcctaatggc cctgtagtgg cttctacacc
     1981 atctgtatga tgtacttcag gatgtcctgg tgttttagaa tcccattgac ggaattgttt
     2041 caaatcatcg attgtcactt gatagccagc taaatgaagc aagctataca acattgctga
     2101 cccatgtcct gccgaaagaa cgaaacggtc gcggtcagcc cagttttttg atgtcgttgg
     2161 atttactttc aggtgttttg tccataacgc atatgccata ggtgctgcgc ccatcggcaa
     2221 tccaggatgt cctgagtttg ctttttggac tgcttcaata cttaatgtac gaattgtgtt
     2281 aacgccaagt tggtcaattt tgtcaaacaa aataacccct cctattaatt taggtttttt
     2341 ttacctttat ttcttacact ttcattctat aagatgtcac acgaaaaagc aatctttttc
     2401 agtatttttt actaaaagtt gtcatttcgg ttgtgtaagc cttttttctt ttggatttct
     2461 tttaattttt ccggggtcac gtcattgccc tcaggatcga cgactttcat tccttcgata
     2521 tgatgacgca taccggaacg aaaagcttcc aaatactctt tgcgcaagtt gatttgttct
     2581 tgttcttcgg cttttgagag accattttcc ttctttttac gtgcaagttc attgatccgc
     2641 tctatttttt caggtgacag catgctcagc cctcctttta ctctccttca tattatataa
     2701 aaactgaaaa aaaaacaact agataaacta aataaaatac gaacattcgt ttgatttagc
     2761 gctttttata tggtactatt tatttatcaa gtgaaagaag gtgtaccttg tggcccgaca
     2821 aactgaatca agacaaatcg aagtcttacg atatatccat gaacaagtaa cccaaaaagg
     2881 atacccgccg actgtccgtg aaatcgggga agctgttcag ctttcttcta cttctactgt
     2941 tcacggtcat cttgcacggc ttgaaaaaaa agggtttatc caaagagatc cgacgaaacc
     3001 tcgtgcaatc gagttgacaa aacaaggttt agaaaagatt gggattcgtc caacaactat
     3061 tccagtactt ggcgtcgtaa cagctggaga acccatttta gcagtagagg atgcttcaga
     3121 cttctttcca atccctccta atctttcttc cgaagaaggc agtctcttta tgctgacaat
     3181 tcgtggagat agtatgatca atgctgggat attggatggt gacaacgtga tcgttcggaa
     3241 gcaagaaact gctcaaaatg gcgatattgt cattgcaatg actgccgagg acgaagcgac
     3301 ttgcaaacga ttctataaag aaaaaaactt tttccgtctg cagcctgaaa acgacttgct
     3361 cgaaccaatc atcttagatc aagtatctat attaggacga gtagtaggac tttatcgtag
     3421 tcatatctat taattgctac taattaaaag aaaacttttt aaaaagagtt tgagacgttg
     3481 gtctcaaact cttttttcgc ccgggttctt cttctttcaa atttttatca cttccctctt
     3541 tacaaaccga acaaatattc gtatactaaa tatacgaaca attgttcgca taaattaaag
     3601 aggtgagaat tcatgaaatc aatgagacgt cttggcttag tattaactgt tttgttttta
     3661 ggtattatta ttggtaattt cggcatacgt ccagctttac ttgtgtttgt tccgttattt
     3721 actttgtggt tcatgttgtg ggatgaaaag aagtaccgtc aatctgagcg gcgcagaatt
     3781 catgaagatc atttctatca tgagccctac tacagatcca ggcaatcata ataaggattg
     3841 actgctaatt attcaaaaga caagaagtta aacttttacg ggggtgtgac aaaaatcggt
     3901 cacatccttt ttttcgaata aacggtgttc taaagccagt ttttcggtat taagtcaaat
     3961 tgcggaaaaa gtgatttttt aaacttctac tggtctttgc ttgtttccat ttataaaaaa
     4021 atagcgaccc tcaaaagtca gatagagaat ctgacttttg agggtcgctt taaaatcgct
     4081 tctttaagaa taacatcaag aatagcttct tttttctgct taatattcca ttaatgtaat
     4141 gatgtcatat cctttgattt tgtcgcgtcc atggagatcc atcaattcga tcaaaaatgc
     4201 gcagcctacg actacaccgc ctaattgttc gatcagttcg atcgtcgctt tgattgttcc
     4261 tccagtagct agcaaatcgt cacaaatcag cacacgttga cctggtttaa tggcatcctt
     4321 atgcaatgtc aatgtatctg ttccatattc taatccataa gtcacttcga tggtttctcg
     4381 aggtaacttc ccttttttgc ggactggggc aaaaccgact cctaactcat aagcaacagg
     4441 acatcctacg atgaatccac gggcttctgg cccaactacc atatcaattc gtttttcttt
     4501 tgcataattt acgatttgtt tagtagcttc ccgataagcg tcaccatctg ccatcagtgg
     4561 ggaaatatct cgaaacacaa ttccttttga cggatagtcg ggaatactgg caatataatc
     4621 ttttaaatcc actctttgtt tcctcctagg ttgttaacca tttctttatg gtaggaatat
     4681 cgctcaaaag caaaaattcc tcaactttga ttttattgag ccgtcgctgg tataatctgc
     4741 tctcagtcaa aggatgactt tctgggctag cgactttttg taaaacaccg ttctctattg
     4801 taacaaattt caattcaaaa aacacctgta tcataaaaat taataatttt tcagggatac
     4861 gcaaatattg agctattgct tttattttat gacgtatatc aattcgttct tgagaatgga
     4921 tcaatttaaa caattttgcg tactgttctc ttgtgcctac cccattcaaa taggcctcat
     4981 caggtgaaat ccccatcaga taaacacgat tgaatgaata agaccgaaaa acttctttca
     5041 gatccgctaa atcatctgga caatccataa caactagaga gtttggtttt cttttttcaa
     5101 ttattgcctt tacagaatct agtccttcga aaacaatcgc agcttcctgg ataatctcag
     5161 gcacgaattt caacgtcgaa gaatcaaatg ctaaataaag gctaccttcc gacgttcggc
     5221 tctgttcacg gaaacgtttt gcccgccaat caaatacttg cagaccaccc acagaaaaat
     5281 cagataccat cagctgcggt ttcttacgcc cgttccattc attgatcgaa agctttccta
     5341 ccacatctac ctgattattc agcagttctt cttcttgagt accaaaacca aaagcgaccg
     5401 catcaagctg actcgatgca tcggaaagga caaatttcag atgctgttgg tttgctccga
     5461 ttcttttgat attttctgct tggacttgtc gaaataaaaa ctgaggcaaa ggattatccg
     5521 taccaaatgg cgccaatagt ttcagctcat caattcgctc tactgttact tcatttggca
     5581 gcaatacttc atcaattctc aactcaggcc ctctcatcaa gtcaatttga tgatcgacta
     5641 tatattggtt catcttctcc tgtagtatag tgacattttc acttggcatc gttaaaccga
     5701 ctgctgcatg atgtcctccg aagtaagtaa ataaatctcg catctggtca agcatttcaa
     5761 aaagattcaa ggcttcaata ctacgtccag atcctttagc tgatccatct tcttttaaag
     5821 taaggacaag cgttggttta cccgtttcat tcattatttt cccagcaaca atgcccaaaa
     5881 cgccttcatg ccatcctcgg tttaccaata gatggatatg attctcctta ttgaccatcg
     5941 ctaatgcttc ttgtgttatc tgctctacga ttgcttttct ttcttcattg atctcattca
     6001 atttttttgc ttgaacggat gcttcctctt catcaaacgt cgcaagtaac gagacggcag
     6061 gacgtggatc gcccatccga ccgattgcat ttagtctggg agcaatcgaa aaaccgatcg
     6121 ttgtttcgtc tgcgtccctc atttttacgc cgctttcttc aaatagcgcc tgaagtccta
     6181 tccgttctga atgatgaatg gcatcgattc ctaatgaaac aaggattcga ttctcatcag
     6241 tcaatgaaac taaatcggcg atcgttccta tcgctactaa atccaaaaat tctgcaggcg
     6301 gttcttccaa taatgcagaa gctactttga atgctactcc gactcctgcc aaatctggaa
     6361 atggatagtg ccccgctgga tgacgagggt gaacaatcgc ataagcgtct ggtaaattct
     6421 caggtagttc gtgatgatct gtaatgatca cgtctacacc agcttgctga gcgtatgcta
     6481 ccgcatcatt tcctgctacc ccgttatcaa cagtgatgat caactggatt ccttcttcga
     6541 tcttttcttg gtataccgat tggtttggtc catacccatg ctcaaatcga tttggcagga
     6601 atacttctac ttctgcacca agtaattcca gtgtttcttt cattaccgtt gcactggtga
     6661 tcccatctgc gtcataatca ccgtaaacga ggattttttc tccactgatt acagcttctt
     6721 ggattcggct gattgcttta tccatatcgt gtatcagata cggatcatgt aattcttgtt
     6781 cctttggatg taaaaatcgg ttgatatcct tttcctcatg gtaacctctt tgccacagta
     6841 acttaccgac aaatggagat aatttcagtt cacggatcat ttggagaaag gattcagaag
     6901 gctctgtctc gcttaacttc caatcaaaat ttgcttgtct cactttctca ctcctaactt
     6961 agttattata gcaaaggaaa tcagcaaaaa aaagcagaaa gaaagaataa aaacagatca
     7021 gatccgctag acagatgttc agcaggacct gatcttttgt acgaccattt atgaaatatc
     7081 cctattagtc aaaataattg aagctttgtt tgggcgattg agttttttgt tcttccggcg
     7141 ctttcaattg actgatttgt gcggttttcg cttgtaactc cgcctgataa gcagcttcca
     7201 actccgcacg ttcgtttcta aactcgcgtt gctgttcttc ttttgcttct tcaattttct
     7261 tttgaagttc tgtttgatat tctgacattt cctgagtcag ctgtttgatt tgcttttttt
     7321 gctgccatag tgttgccgaa gcagtcaaaa atacaatcag tgcaccaatc agcgcagaac
     7381 cgatgatcac taagatcaat ggcgcagaaa aggaagtgaa gccaaaactt acaggaacat
     7441 ttttattgtt caatacagca aaaagaacaa taatcaaaac taaaacaaat ccaagaatca
     7501 cattgctttg ctttttcaat tccttcacct actttttgtt aaaaattccg ctggctaaat
     7561 aatcaccaat cgttggaaac agcgtataaa attttgatgc aacttctaaa ataaacggtt
     7621 gattgatttc ccgttttgga tgtctcatat tgcggacgat catttttgcc aacttggcag
     7681 gttctaaaac gatttgtccg actttttcca aatagcttcc gcttggatca gccttatcga
     7741 agaaatttgt ctcgattgga cctggattca ctgtcgtaac agctacgcca agtggtttca
     7801 attctaatcg caaagcgttg gagaagccaa ggactgcaaa tttcgtggca gaataaaccg
     7861 ttgattttgc agttgccatt tttccagcca tagaggcgat attaatgata tgaccttgtc
     7921 ttctttctgc catatcgatc gcaaattttt gtgtcaaaac catcattcct aagatgttga
     7981 cttcaaacat atcgtatgct tttccaaggt cgaatgtaag gaagtcttcg aaaatcccaa
     8041 aacccgcatt gtttaccaga atatcgacgc gcccgacttc ttccatcacc ttttcatata
     8101 attcgtccac gctttcagga ttgctgacgt ctaccttgaa agcataagcc tctttgccgc
     8161 tcaagcgtct gcactcgtct ctgacacctt ctacaaatgc cattcttcgt gcgcaactta
     8221 taacgattgc tccttgcttt gctgcttcat aacagatttg tgcgcccaga ccgcctgaac
     8281 tacctgttac taaaacaact ttatcttcta aattcatgcc tcttcctcct cgcgaaatgg
     8341 aatctcaatg ctatcaaaat ctttcataat cgtcgtgttt tcaaacactt cttgtgcttc
     8401 tttttcaagc tctaaagctg cttttcctaa atatctcgca ctgatatgag taagtagcag
     8461 ccgttttgcc tgcgcttctt ttgcaacttc agcagcttga tgagtcgtgg aatggaaata
     8521 gttgtgcgcc attcttgctt catctttatt aaatgtgctt tcatgtacca gaatatcgct
     8581 attttccgca agcacaacgg aattatgtgt ttttctcgta tcgcctaaaa tagtcacgat
     8641 tcgtcctttt ttatctggac ctacgaaatc ttttccattg atcgtccgcc catcttctag
     8701 ttggatcgtt tcaccttgct tcagtttgcc gtacagtggt ccagctggaa tacctaattc
     8761 ttttagacga tccacctgca attctccttt atgatcatgt tcaaccacac gataaccaaa
     8821 actgtcgatc ccatgattta gtttttttgt ataaacagaa aattgctgat cagtaaaaat
     8881 cggggctgtt tcatttagct caatgaattt cagcggataa cttaaacgcg tttgcgaaat
     8941 acctaaagag acttttatgt attcctctat cccttttggc ccgtaaatct ctaatggcgt
     9001 atccccacct tgaaatgacc ggctgctgat gagcccggga agtccaaaaa tatgatcgcc
     9061 gtgtaaatgt gtaatgaaaa ttttaccgat ttttctcgga cgaatagtcg ttcgcaaaat
     9121 ctgcatttgc gtgccttcgc cacagtcgaa caaccaaact tcattgcgct cgtctaaaag
     9181 tttcaatgct atacttgtca catttctatg ttttgcagga acacctgcac ctgttcctaa
     9241 aaattgtaat tccataatct acctgacttt ctttaagttc ctctttaatt ttagggtaaa
     9301 aagaagggat aatcaaccgt aagtcgagaa tgggttcaaa ttaaaaataa cgttctaaag
     9361 cgatagcgtt ttctttgaaa attactataa aaacagcagc gaacccgttt gagatcctct
     9421 gctgtttttt attaatttga agatccagaa ctttcattgt cgttggaagt ttgcggatgt
     9481 ttgtccttcc aagtcttttg tgcagcttct ttcgcatgat tttcagcttc tttcgcatca
     9541 tgcacatctt tttcgacatc ttcataatcc aagcgagtaa tttctccgcc taattccatc
     9601 attaccaatt gattcagtgg gcgattgtcc tcttcatcag caatcaagat cagtcctgta
     9661 gctccttcac cgattttatt tcctacaaag tcgaacacgg tctgggcatc ttttatttct
     9721 ttggcatctt gtgtcgcacc gatcattcct ccggcaaacc atcctaataa aatccctagc
     9781 ggaccaccta atacaccaac caacattccg attaatccac ccgtagaaga tttgttatta
     9841 ccggtaaagt cgataaaatc ttctattttg aattgatgtg tcccattagg atcatggctg
     9901 acaactgcca tctgttctcc tttaatcgaa cgaacagcat gtaacttttt gatttctgaa
     9961 aatgcctgat atgcctggct ttctacttga aaatttaaaa tgattactcg tttgctcatt
    10021 aaaaacgccc ctttcatacc ttcttgttat ttattttacc tttaattgaa aaataagaaa
    10081 agaaaagcac tctttttttc caataaaata gggctgtgac aaaagtcgta tcacagctcc
    10141 tttttccgaa taaacggcgt tcttgaagct gatcacttct gtcacaacct cccttaaatc
    10201 tgaatctata ttttttggta tttgttttta tttgcttgcc tattcgacaa attcaaattc
    10261 aaactcaccg atacgtacaa ggtcgccatc tttcgcaccg cgtgcccgaa gagcttcatc
    10321 cacacccata ccacgtaatt gacgagcaaa acgcatcact gtttcatcat gatcgaaatt
    10381 cgtcatttgg aataattttt cgatcttctc accagataag atccacgttg catctgaatc
    10441 acgatcgatt tggaactcag gaccttctgg attgaatcca tagtgtacag tttcttcttc
    10501 cagttcttct tcgtacaatg ggaattctgg cgttacttct aacaaatctg ctgtagcatt
    10561 caataacgcg tccaatcctt gtcgagtcac tccagaaatt ggaaatactg ggatgtcatc
    10621 agcaaattca tcttcttttt ccttgttcag ctgttctttg aatttcacta gattttccgg
    10681 tgcatccggc atatccattt tgtttgccac gatgatttgc ggacgctcta gcaagcgtaa
    10741 gttataagta gataattctt tgttgatggc aagatagtct tcataaggat ctcgtccttc
    10801 catcccactc atatcgatca catgtaaaat tactcgtgtt ctttcgatat gacgaaggaa
    10861 ttgtgtaccc agcccaacac cttgcgaagc tccttcaatc aagcctggca agtctgctgc
    10921 ggcaaaactt ctgccatcac ttgtagtaac cattcctaga ttaggtacga gcgtcgtgaa
    10981 atgataagcg ccgatttttg gacgagcaga tgaaataacg gatagtaaag tcgatttccc
    11041 gacagatgga aaacctacaa gtccaacgtc tgccaatact ttcaattcta gttcgatttt
    11101 tcgctcttgt cctggttcac cgttttcagc aatttccggt gcaggattcc gaggagaggc
    11161 aaaacggata ttccctcgtc caccgcgtcc accttttgcc acaactagtg tttgaccatt
    11221 ttcaattaaa tctcctaaaa gagcaccagt ctccgcatct cggacagtgg ttccttgtgg
    11281 tactttaaca taggtatctt ctgaaccacg accgtgcatc cctttactca tcccattttc
    11341 acctggctgc gctttgaaat ggcgattgaa ccgaaaatcc atcaatgtac gtaacccttc
    11401 atcgacgatc aagatgacat cgccgccacg accaccgtct ccgccagccg gtccgccgtc
    11461 aggaacgtat ttttcgcgac gaaatgcaac cattccgtct ccgcccttgc cagctttcac
    11521 atcgatcgtt acttgatcta aaaacatgga cataattaag tcctcctatt attcatttat
    11581 ttatttgact gcctgaattt ccacaataaa caaaagtctt acttatttta tcggtgaaat
    11641 ctgattttgt ctatcatttc agcaaaaaga ttgtttacct tcttcttttt ttatcatttg
    11701 gtaaggtttc caaccttttt tctgctacca agtataattt tttgacaaaa tattctttgc
    11761 gtagcagaat aacagtgtat taattgtatt aattgaggat gccgtttcac agcaccccta
    11821 ataaaggagg aacttttgat atgacagtaa aagtaggtat taacggattt gggcgtattg
    11881 gccgtctggc tttccgccgc atcaaagaag tctcagatga tattgaagta gtagcaatca
    11941 acgacttaac aagtccaact atgttagcac aattattgca atttgactct actcatggta
    12001 cttatcctgg cacagttact gcaacagaag actcaatcgt agtggatggc gaagcaactc
    12061 gtgtttatgc agaacccgac gcaagcaaaa tcccttgggc aaaagaaaac ggtgtagata
    12121 tcgtactaga atgtactggt ttttatacat ctgaagaaaa agcaaaagcg catttagatg
    12181 ctggtgtcaa acgagtagtt atttctgccc cagctggtgc tatgaaaacc atcgtctata
    12241 acgtcaatga tgatacatta gatgaaaacg acaaaatcat ctcagcaggt tcatgtacaa
    12301 caaactgttt agcacctatg gcttatttct taaataatga atttgggatc gaagttggta
    12361 caatgacaac agttcatgcc tacacttcca cacaaatgct cttagatgga cctgtaaaag
    12421 gcggtaattt gcgtgctgct cgttcagctg ctgacaatac tatcccacat tctactggtg
    12481 ctgcgaaagc aatcggctta gttatccctg agctacaagg aaaattacaa ggacacgcac
    12541 aacgtgttcc agtcgttgat ggttcattaa ctgaattggt ttcagtattg aaaacaaaag
    12601 taactgctga tcaagtaaac gaagcaatca aaaaacacac agttgacaat ccttcattcg
    12661 ggtatgatga ccgtcaaatc gtttctagcg atgtcattgg aacaactcaa ggttcgatct
    12721 ttgacccaac gcaaacagaa gtaacaactg ctggtgattt ccaacttgtt aaaactgttg
    12781 catggtatga caatgagtat ggatttactt gccaaatgat tcgcttacta gaaaaattcg
    12841 ctaacttgta atttgataga tcaatcgata aaagaagcct ttgactggat ccagtcaaag
    12901 gcttctttct tttcatcccc tacttttaaa ctcgttcaat gaacatattg gcttaaaaat
    12961 tgttggacta cttgctgata tttttcagga tctgtcttgt atgcttccgc atgctcggct
    13021 ccttttacaa catacttttc ttttggtccc ttcgttgcac gatagacctt atctaacatt
    13081 tcaaatggta caaatgtatc ttcgtctcca tgtataaaga gcattgggcg ctggtttttc
    13141 ttcaattgtt ccactgcatt tgcttcaccg aaaaagtagc cagcacggac ttttgtcact
    13201 agactcgtta cttgcataag cggaaatgct ggaagactaa acatatcgtc tagctgataa
    13261 gcaagctcat catgaacgga agaataaccg caatcttcaa tgattgcttt cacattatct
    13321 ggtagttttt ctccgctagt catcataact gttgctgctc ccatgctgac accgtataga
    13381 acgatttctt gcgattttcc attgttttct agcactttgt ttatccactg tacatagtct
    13441 ttacgctctg gccatccaaa tccaatgtaa tccccttcgc tttttccatg tcctcttgca
    13501 tcaggaacaa ggacattgta acccatatca tgaaacatct ttgcgtaatt ggtcatcgtt
    13561 tcagcgtttc ccatatagcc atgagccaca agagctgttt tttcactttt cgtttcagca
    13621 ggaagataaa tcgctgataa tttcagaccg tcttttgaag tgatggtcca atcgcttcgg
    13681 ttttcttttg aactaaacca tttatgtgca tccgtttctt tggatgtctg tgttgtacca
    13741 ggtgtatctt cgtctaaaaa gtctttctca gaaggcacaa cagcataatt ataaaaataa
    13801 ttccctgcaa acactaacgc aacaatcaga gctaaagcga cgcctaaaag gattttcatc
    13861 caaagtttca taaatcatcc atctcatttc tcttttttca tataaccttg aattagtata
    13921 atcaaagaat ctctactatg caatgttcat ttttataac
//
LOCUS       DANKKG010000059        13836 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000059 DANKKG010000000
VERSION     DANKKG010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13836
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.059"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            33..1772
                     /gene="yfcC"
                     /locus_tag="PW004_001544"
     CDS             33..1772
                     /gene="yfcC"
                     /locus_tag="PW004_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HDL2726523.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            1929..3113
                     /gene="iadA"
                     /locus_tag="PW004_001545"
     CDS             1929..3113
                     /gene="iadA"
                     /locus_tag="PW004_001545"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HDL2726524.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3224..3427
                     /locus_tag="PW004_001546"
     CDS             3224..3427
                     /locus_tag="PW004_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDL2726525.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3528..4190)
                     /locus_tag="PW004_001547"
     CDS             complement(3528..4190)
                     /locus_tag="PW004_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2726526.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4203..5705)
                     /locus_tag="PW004_001548"
     CDS             complement(4203..5705)
                     /locus_tag="PW004_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2726527.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6253..7932
                     /locus_tag="PW004_001549"
     CDS             6253..7932
                     /locus_tag="PW004_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDL2726528.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8059..8538
                     /locus_tag="PW004_001550"
     CDS             8059..8538
                     /locus_tag="PW004_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HDL2726529.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8757..9437
                     /locus_tag="PW004_001551"
     CDS             8757..9437
                     /locus_tag="PW004_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDL2726530.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9445..10776
                     /locus_tag="PW004_001552"
     CDS             9445..10776
                     /locus_tag="PW004_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDL2726531.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10791..11753
                     /locus_tag="PW004_001553"
     CDS             10791..11753
                     /locus_tag="PW004_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HDL2726532.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11864..12202
                     /locus_tag="PW004_001554"
     CDS             11864..12202
                     /locus_tag="PW004_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HDL2726533.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12281..12988
                     /locus_tag="PW004_001555"
     CDS             12281..12988
                     /locus_tag="PW004_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HDL2726534.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(13091..>13836)
                     /locus_tag="PW004_001556"
     CDS             complement(13091..>13836)
                     /locus_tag="PW004_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HDL2726535.1"
                     /translation="TSFSVSRPCEETETERAYFNYFFYCFFPNQISFRQKQEIGFLFS
                     TSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFFELLGESSSSFSTLC
                     FPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQHLLQTETKEQLLIH
                     LQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHEKISDETETFYLDTT
                     HNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         4397 a         2432 c         3013 g         3994 t
ORIGIN      
        1 aaattactga aaaaagaaag gaagaaataa atttgaagaa aacaaattct tcaggaaaac
       61 cgaaacgcga cttcactaaa ttatcgacac cccatacgta tgtcattatt tttggtgtag
      121 tcatctttgc atggatactg acttttgtcg ttccagcagg aaagttcagc acgcaagata
      181 ttgaatacaa agatgcaaat ggcgaaacaa gtactcgtac tgttttacgt caagattcct
      241 tccgttatgc ttacgagctg gataagtcgt atgtgtttga tcaattagaa gaactgcaag
      301 atcatccagc agaacgtgaa aaactggatg ttccggaaaa aggattggaa aaagtcattg
      361 ctgacggaga aaaaaatctt acgcaggaaa aactagatga aatctccttg acagatgatg
      421 tgctttacga tcagtacggc gaaaatatct atgatacttc taaaaaattg cataaaacag
      481 ccaaaatatg gggaacagat gattttggcg gctttggttt cttaaatttt gtttttgaag
      541 gactagtatc tggagataag tatggatctg ctgtagggat cgctgcattg atccttgttg
      601 ttggaggtgc ttttggtatt attatgcgta ccggagcgat cgacgcaggt atctatgcgt
      661 ttatcagtaa aactaaggga ctggagcgtt tagctttacc gctattattc tttgcttttt
      721 cattcggtgg cgctacattc gggatggcag aagaggtaat tccattctca atggtgatgg
      781 ttccatttgt cattgcgtta ggatatgact cgatcgtagc agtaaccgtg acttatgtcg
      841 catctcaagt cggaaatgcg acctcttgga tgagcccgtt tagcgtagcg gtggctcaag
      901 ggattgctgg aatccccgtg ttgtctggtg cgacattccg attgattatg tgggtagttg
      961 ttactgcatt agccgctggt tatatgatga tatatgcaga aaaaatccgt aaaaaaccag
     1021 aaagttcgtt gacttacaaa tcagatgatt atttccgttc tcatatcaaa aagacatctg
     1081 atgaaaataa aaaattcatg cttgggcata aattgatctt attagagatg ttagttgtcc
     1141 ttgtctggat cgtttggggc gtgactcaaa aaggctatta cattccagaa atcgcttctc
     1201 aattctttgt gatgggatta gttgctggaa tcattgctgt tcttttcaaa ttagatggaa
     1261 tgaaagtcaa tgacatcgct tcttctttcc aatctggtgc agctgattta gctggtacag
     1321 caatcgtagt aggtatggcc aaagggattc ttcttgtctt gggaggatct gatgcatctg
     1381 ttccttcagc tttgaatacg attttgcatg ggatcgggac ggcgttgacc ggtgttccag
     1441 ctgtcattgg tgcttgggcg atgtacatct tccaaagttt gttcaacttg gttgttacat
     1501 caaattcagg acaagcagct ttgactatgc caatcatggc tccactggca gatttagtag
     1561 gtgtatccag acaagtagca gttcttgctt atcagcttgg ttcaggtttt atggacgctt
     1621 ttacacccgt atcagccagc ttgattggtg tgctaggtgt tgcacggatc gaatgggcaa
     1681 aatgggcaag attccaaatc aagatgcaag gattcctgtt tgttttagga actattttca
     1741 tcatgattgc cattgcaata ggtctgcagt agtactggag acctgtaaat aaaaaagtaa
     1801 tccaaacagt agataaggac tccaagaacc gtcattttac ggtaaaatca aaggagtaaa
     1861 gatcctatcg tttggattat tttttaagat cctatcatca gttatactta aagaaagagg
     1921 aaaaatgtat gaaaatcatt cgacaaattg aagtgtttgc accagattac ttaggaaaaa
     1981 tggatgtact gattgccggt gggaagattt tggctgtaga agaacagcta gaaggcggct
     2041 acgaaggagt ggaagtagaa gaactttcag gtgagggaaa agtactaacc ccgggtttca
     2101 ttgactgtca tttccatatt ttaggtggag gaggagaagg cggttatcaa aatcgaacgc
     2161 cagaagtcac attgagccaa ttgactacag ctggtgtaac gacagttgtc ggttgtttgg
     2221 gaacagacgg agaaggtaga gatatgactg cgttgatcag caaagcgaaa ggtctggaag
     2281 cagaaggaat ctccacttat gtatacgaag gttcgtaccg attgccggta aaaacagtaa
     2341 cagattctat catcaaagac tttttgacaa ttgataaaat tatcggaatc ggagaaatcg
     2401 cagtatccga tcaccgttct tctcagccaa gttttgaaga gttcgctcgt gcagtagcag
     2461 atgctcgtgt aggaggcatg ttatccggaa aagctgggat catcaatgtc cacttaggcg
     2521 gaggaaaaag aaagctagag ctattgacac gaatcgtaga agaaacagaa attccgatca
     2581 cacaattttt gcctactcat gcaaatcgca caccagaatt atttgaagct tgcgtggcat
     2641 ttgccaaacg tggtggcacg attgacttca ctgctagcga agatcctgat ttttgggaga
     2701 agacagatgg tgaagttcgc ttcagcaaag cactgaaacg attgatcgag gaagatgttt
     2761 ctttagataa tttcacgatg acctcagatg gacaaggaag cttgccttat tttgatgaaa
     2821 acaatcattt cctagggtta ggagtaggaa gtgccaaggc attgctagta gggatcaaag
     2881 aagctgtgca aaaagaaagt atccctttgg aaatcgcctt acgagctata accagtaatc
     2941 cagcacgtat attgaaattg gataaaaaag gaaaaatcga aattggtgct gatgcagatc
     3001 tgtgtatctt ggataaagaa acgcttgata ttgatacagt tatcgctaaa ggagaaataa
     3061 tggtccaaga aaaagaagtc aaagtatggg ggactttcga aaaaagtttt taatcaacag
     3121 ctctaactat cttttttaga aaacgagcgt tctgtttgtc tgtatctgtt gttgatgtta
     3181 ccctgaaggt agatcaataa acaaactaag aggaggaagc acaatggcag atttaaaagg
     3241 acgttctaaa gatatgaaag ataaagtagt cggtgaagga aaagaagcat acggcaaagc
     3301 aacagacgac aagagcaaag aaacagaagg gaaagcacaa tcactaggtg ctgacatcaa
     3361 agaaaaagct cgaaatttag gcgacgaagt caaagacaag ttttctcgaa aagacgaaaa
     3421 aaaataaaaa ccacttatta ctaattgaat caagcaaata aaaaatccga accatatctg
     3481 cgagacagat atggttcgga ttttttattt gatcattatc ctgtttttta aacttcgatt
     3541 ttttgaaatt ttttattttt tagttcaaag acttcatcac agctttttgc tacttcaatc
     3601 tcatgagtca cgatgataat acagcgattt tgttcatggg cgatttcttg gaaaagtttg
     3661 acgatatctt gtgaagttgt ttcatctaga tttccagtag gctcatccgc tacgattagt
     3721 tgatgttcac agcagatagc acgaacgata gcgactcgct gctgctgacc tcccgaaagt
     3781 ttggtcacct gtttttttgc taatatttca tcaattccta cacgagccag tcgatctaaa
     3841 gcaacttgtc gataattctc ttgcttggtc ttagtgattt ccattgctgc tatgacgttg
     3901 tctagagccg ataaataagg aagcaagttg taagcttgga aaataatcga gacatcattt
     3961 ttacgataac ttcttaaccc gattttgttc agtgaacgat cttgatagta tacctttcct
     4021 tcttttggtg catctagtcc agaaataagc gaaagaaaag tcgtttttcc agaaccactt
     4081 gctcctaaaa tagcgtacat ttttccagct tcgaaattca gttctacatc tttataaagc
     4141 gcatcttgcg gatcttgata ccaaaatccc aattgttctg tccgtaacat caaattcctc
     4201 ctttagttta ataggatttt tcttgggttt aatcttaata tccctataga agctaaaaag
     4261 acagaaaaca gactgattcc aaatgctact ccggctaata gtcccaattg tgcaggtttg
     4321 acattgattt ccaattcgct gacttgatca gaaacagcaa atggattatt cgtggttcct
     4381 tgttgaccat cgcgttgacc gccttgttct tgtctgttac ctggcatttg tccaccgcca
     4441 ggagcttgct ggttttgtgt cgtggtttct gtctgttggt caagtagctg ctgtcccacg
     4501 gcattcgcga cgacgtttcc gctaagcgaa gcaactccta atgcaagaat catacaaata
     4561 gccacttcgg taaacaattg gagaatgatt ttacttctgg attctcctaa agatagcaag
     4621 acgcctaact catgccgacg ctcccgaatg gtaatcataa tgatcagagt aagtatgata
     4681 ataccggcta cagccactag taaaacaaca tttttagaaa aacttgccac gttgtttaaa
     4741 ggttccaaca ttgactgata catggaatca tttgattgta agctaaatgt atctgtgtcg
     4801 atcagcttct cagcttcttt gagaaactca tccattttat ctggatcaga tagagtgtaa
     4861 atagcagaat ctatcgtgtt atcgtcagat gtcccattta gttcatttgc aaaagtgtaa
     4921 gaagtgtaaa gtgtattcga cggattcatg aagttgaatt tcatacccat actgccgctt
     4981 gtttcgctgg attcataaat tcctttgatc gtcatttcat aagtggtatc ttcatcttca
     5041 gtactcgtga ttgtgaaagt atctccgact gccaggtcat ttgtttctgc tagagtagaa
     5101 tcaatgacta cattgtttgt gcctttgtcg ctgtcatcta ttccttcacc atccgtgatt
     5161 tttgctgttc catcagaaaa attactattt tgtgcagttg ctgacacacc actgatttga
     5221 aagtctgctt gagtcatttg cggattcccg cccattcctc cagcaggttc accgcctgca
     5281 tcagtagttt ctgaactgtc ttcacttgaa tcagaactag tgatggcagt tattccatct
     5341 tttgccatag ctgaggcgga actttcaaag gaataactct ttacattatc taattcagct
     5401 attttttccg cgtccgttaa ggaaactggt gtaagagaaa aactgcctgg atctggacgt
     5461 tcttcagtgg tagaaccgct tgaagaagtt tgttcctgtt ttttaaatga agcttctcga
     5521 ttggcactta aagtcactgt tgctccaact tctttttgtg cttgattcgc tgcttcttct
     5581 gcagcgcttc gaatcgtgag gccagctagc acaaaaataa gaatggctgt gaacacggca
     5641 aacaataaca atgaacgtcc ccattttact tttgtacttt tccatgcacg tttcataaaa
     5701 ttcatatttt gcatccctcc tagtagatgc atttgataat agacaaccta tcttaaagct
     5761 agcttaaatc ttatgctgtt tgcacaccat caacatgagg ttctgtatat ataaaaagtc
     5821 actatagtta ttgcaaaaaa tgtggtaaaa tgaaaaaagt atgtttcaaa ccgacgaaga
     5881 agaaatgaaa gaggaatagc aaaagaaatc ctagatgcta cagagggatt tttacgtgca
     5941 tagttgttct aacaattgct gatactagcg atttagttta tttgttgtat aataatgggg
     6001 aacagctgaa tacttatata agtattctgg ctatagcgtt tcgcaaaaga tccattttgt
     6061 taatgaagta tatccaaaat aaaatgccca tccaactgtc agaacgcgga tgtgctggta
     6121 attttcctgc tgattctcca taagttgaga aatagtgatt ttgcatatgc ttcattggaa
     6181 tggctcatat atttagataa agaagaaaat cgacatcttt aaacttggat gtcataagaa
     6241 gaggtgaagt ttgtgagtac cattaaaatt atcccattag gcggcgttcg tgaaaatgga
     6301 aaaaacatgt atgtcgcaga agtgggcgag gatattttta ttttagattg tgggctaaaa
     6361 tatcctgaaa atgagttgtt ggggatcgac gtcgtaatcc ctgattttac gtatttagaa
     6421 gaaaacattg atcgtgtagc tggtgtcttt ttaacacatg gacatgcaga tgcgatcggg
     6481 gcacttcctt acttgttgtc aaaaatttct gtaccagtat tcgggacaga gttgacgatc
     6541 gagttagcaa aattgaatgt cagtcgtaat gaagcagcca aaggatttaa ggatttccat
     6601 gtggtagatg agcatacaga aatagacttt ggtgaaacag tagtgagctt tttccgtacc
     6661 actcatacga taccagattc agtggggatc aatctgaaaa caaaagaagg tagtatcgtt
     6721 tatacgggag attttaaatt tgaccaaacg gctatcccaa tgtatcaaac agattatgca
     6781 cgattggcgg aaatcggcaa agaaggagtc ttagccttac taagtgaatc ggcaaatgca
     6841 gaaaatcctc agcctgtagc ttctgaagcc caaatagcag atgaagtatt tgatacgatc
     6901 aaatattggg aagggcgtat catcgtcgct tgtgtagcaa gtaatttgca acgagtgcaa
     6961 caagtattgg atgcagcaga aaaagcacaa cgaaaagttg tcttgactgg tcaagatttc
     7021 gaacgaatta ttcgcacagc gatgcgttta gaaaaacttc aattaccaag tgaagatctg
     7081 cttgttacgt tgaaagacat gaaaaactat gatccagaag aactgatcat tttagaaact
     7141 ggccgaatgg gtgaacccat caagtctctg caaaaaatgg caaacggtac acaccgtaat
     7201 gtcaagattg aagaaggaga tttggtttat atcactacta cgccaagcat cgcgatggaa
     7261 accatcgtgg cgaaaacgga agatatcatt tatcgagctg gtggaacagt gaagttgatc
     7321 tctgagaata tgcgcgtttc tggccatgcc aatcctaatg acttgcaatt aatgatcaat
     7381 ttgatgaaac caacatattt tgtgccagtt caaggagagt atcgtgaatt agctgctcat
     7441 gcagatttgg ctcatgcagt agggatgcca tacaaaaata tctatattac tggacgcgga
     7501 gacgtgctgg aatataagaa caagcgtatg agtgttgcag gaactacaag tgcagaaaat
     7561 gtaatgattg atggattagg cataggtgat atcggaaaca ttgttttacg tgatcgaaaa
     7621 atcttgtcag aagacgggat tttcgtagcg gttgtcacta tcagtcggcg agaaaagaaa
     7681 atcatttcaa aacctcaaat tacttcacgg ggattcgttt acgtaaaagc tagccgtgat
     7741 ctgatgaaag aaagcagcaa tatgattgaa acaatcgttg agaaacattt agaaagcaat
     7801 gattttgagt ggagcaaatt aaagcaagac atccgagatc aattaggccg ttacttattt
     7861 gaacaaacaa aacgccgccc agtgatcttg ccagtcatta tggaagctac tcaacgtcga
     7921 aataaaaaat aaacaaaaag agtaagatga acctctctat agagtgctcg tcttgctctt
     7981 tttttgcgaa aaacaaataa tttttgaata cagcatccct cgctttataa taaacgatag
     8041 aataaaggga gtgaatcaat gatgagtcaa acaaaagtcg aacgttattt agaagaacaa
     8101 aagatcagct atcaaccact tgattttagt catgtggtag aagaatcttt cacagaagaa
     8161 ctacaaaaaa gagggattga tccagctttg atatgtaaaa ctctcgtagt gaaaggaaac
     8221 aaaactgggg tgatcatcgc attgattcct ttgaacgatc acttagatta caaaaaaacg
     8281 agacaagtga cgaaagatcg taaagttggt tttcctggaa tggattttgt attaatgcat
     8341 acaggatatc cccacggagc aaacacccct attggaataa aactcgcaca tcctgattat
     8401 tgttttttag cagacagttc tttaaaagaa aaagaagaag taattgtgtc aagcggtgaa
     8461 atcgggagaa gccttcggat aaaagtagcc gatttagaaa agatcgtcca tcctatttac
     8521 gctgatttaa ttagttaagg gacgagaaga gaagctaagc agagcagaga cctcaaactt
     8581 gagattatat ctgcttggta cttggtgcat cctgtattat ctgttacaat tgctttgtac
     8641 atactgtact atcaatccaa tgaaaatata tttagcagct atattttttc attgatgtgc
     8701 aagtgatgaa atcgttttta ataaaaaaga tacagagaaa acgcgaggtg taaaatatga
     8761 aaattacgta tcatggacat tcctgtttaa caattgaaat ggaaaatggc caacgcgtga
     8821 tgattgaccc gtttattaca ggaaatccat tgacagactt aactgcagaa gaagtaaaga
     8881 cagattggtt gctgattact catggtcata gtgaccacat aggagatatg attcctattg
     8941 caaaagaaaa tcaagccaca attatcagta ttgttgagat tgctgattat gcgaacagca
     9001 gaggagtaga ttcattcggt atgaatatcg gtggaaaaca tgcgtttcca ttcggcgtgg
     9061 taaaattcgt acatgcacag catagttcaa gctatgaagt agacggtatc gttcagtata
     9121 tgggcgagcc tagcggaatt attattcaag cagaaggaaa aacgatttat catgcagggg
     9181 atactgctta tttcagtgat ctaggattgc ttgcagaaga attcgatatt gatgtcgctt
     9241 tcttgccgat tggcgataac tatacgatgg ggccagaaga tgcagcaaag gctgcgcaaa
     9301 caattcgtgc caagaaagta gttccaatcc attacaatac tttccctgtc attgaacaag
     9361 atcccgaatc ttttgtagag ttactggaag atagtgaagg aaaaataatg agcgtctcgg
     9421 aaacgctgga agtgtaggca gaaaatggcg acaaaacatg atttgatttt agaatatatt
     9481 gaaagtttac ctgttggcaa tcgaatttct gtaagaagca tcgcaaaaga attgaatgtc
     9541 agtgaaggaa cagcgtaccg tgcaatcaaa gatgcagaaa atgtcggact agtttcaaca
     9601 attcaacgag tagggaccat ccgtatcgaa cgcaaattaa aaaaacatat tgaacgtttg
     9661 acatttggcg aggtcgtcag gatcattgaa ggggacgttt taggcggggc ggcaggatta
     9721 gacaaggtct tgaataaatt cgtcatcggc gcgatgacta aacatgccat ggaacgctat
     9781 attacgccag gatcactaat gatcgtcggc aaccgggcgg aagtccaaaa attagcattg
     9841 gaagatggcg ccgcagtatt gatcacagga ggattcaata cgactgctga gattgcaaag
     9901 ctagctgatg atcttgaaat gccgatctta cgcacctctt atgatacatt tacagtcgca
     9961 accatgatca accgagcgtt aagcgaccaa ttgatcaaaa aagatatcat gcttgtcagt
    10021 gatatctata tgccagctga aaagactcat tatttgcagc agacagatac gatttctgat
    10081 tatcagcgtt tgtcagaaga aacgcagcat tcgcgttttc cagtcgtcaa ccgccatcat
    10141 cgtttgatgg ggattgttac ggcaaaagat gttttaggga aatcgccaaa ccagatcatc
    10201 gatcgggtga tgacgaaaga accaatcagt gtaaagaaaa caatgagtat tgcctctgtt
    10261 agccatcaaa tgatctggga tgggctagaa gtcatgccgg tcgtttccga tgacttgaca
    10321 ttggaaggct tgattacccg gcaagatgta atgaaagcga tgcagcttgt ccagcgacaa
    10381 ccgcagattg cagatacgat ttcggatcag atatctggaa atatccatac gattgatact
    10441 gaccgtgaag gaaatcgttt ggaaaaccct aagttcaaat tcgtagtagc accacaaatg
    10501 gtcaatggcg ttggaaccat ttcttttggc gtacttagcg aaatcatttc tgatgtcgca
    10561 caaaaaacaa tggtgatgaa ccaaaaacga aatatcttga ttgaacaagt gaacctacat
    10621 tatttgcggc tgatccaatt ggaaagcgaa ttagacattc gtccacgtgt attggaaatc
    10681 ggcagacgtt ccgctaaact ggatatcgaa gtgtttattg aaaacaccat cgtttccaaa
    10741 gcaatcgttg tttgtcaagt aatggaacgt tcttagaaag caggagtgaa atgacagtac
    10801 agcaagaaat agtagataca ataaaaaaat acgataaaat cattatccac aggcatttgc
    10861 gccctgatcc tgatgcatta ggatcacaag taggattagc cgaactatta cgggaaagtt
    10921 tcccagaaaa agaaatacgt caagtaggtg aaacgattga agggcttcgc tttttagcag
    10981 aaatgcaaga agttgaagat gtcttctacc aaggagcgct agtcatcgtg actgatacag
    11041 ctaattcacc cagaatcagt gatcaaagat atcagttggc agaaaaactg atcaaaatcg
    11101 accatcatcc caatgatgac ccttatggag atcttgtgtg ggtaaataca aaagcaagta
    11161 gctgtagtga gatgattgca gaatttgctt tgatgtttcc tgaatcttta aagatgaacg
    11221 ttaaggctgc acggttactt tatgcaggaa tcgtcggaga tacagggcgc tttttgtatc
    11281 cctccacgac gtcccgtaca ttagagattg cttcgatatt gagaaattat ccatttgatg
    11341 caagtgcttt gaacagagag attgaacaaa tgccgatgaa agtcgctaaa ctttccggtt
    11401 atctttatca aaatatccag gtggatgaaa atggtgcagg aagaataatc cttccgcaag
    11461 aaattcttga taaatatgga atcgatgatt cagagacagc ttcgatcgtt tcacttcctg
    11521 gagtgattga cgaagtgctg gcttggggga tttttgttca gcagccttca gggtattacc
    11581 gcgtacgttt acgttctaag gggccgatca tcaatgaatt ggctaaacgc catcatggag
    11641 gaggccatcc attagcaagt ggtgcgaatg caaaagattt agcagaagta gaggaaatct
    11701 ataaagagat ccaagatata tgccaatcgt ataagagcag aaaaacagaa tgatacttcg
    11761 ttcgaaaacg tgccttttgt agtaaaaatc acaaatgtat cttttcaaga tcttcttttt
    11821 ccgttacaat agtgctcact aaagatttta aaggagaaag aagatgacat taccaaattg
    11881 cccagaatgt ggctcagagt acacatatga agatcgaggc ttgttgattt gcccagaatg
    11941 cgggaacgaa tggaacaaag aagaacaaga aactgtcata gaagacggat tagtggtatt
    12001 agatgcaaat ggaaaccgtc tgttagaggg cgatagcgtg acagtgatta aagacttgaa
    12061 agtaaaaggt gcgtcaggtg ccattaaaca aggaacaaaa gtcaagaaca tccgtttagt
    12121 agaaggcgat cataatatcg attgcaaggt agaaggattt ggaccgatga aactgaaatc
    12181 ggaatttgtg aaaaagaatt aaagaatatt caaggtcttt tcacattctt ttcctactac
    12241 taatgataaa ataaagtaaa cgaaaaggag tgaaacgaaa atgtatggtt atggaatgta
    12301 tggtttttta gatccgacat ttcttttggt cattcttgga ttagttattt ccggaattgc
    12361 gtcagcgtac gtcaatagta ctttccgaaa atacgatcaa gtgagaagca aaaacaatgt
    12421 gactggtaca caagcagcac aatatatatt acaaagtcaa cagataaata atgtcggtgt
    12481 tcaacaaatt gccggagatc tcacagataa ctacaattca ggtaataaga tgttgagttt
    12541 gtctcaagca actgcacagt ctacttcagt tgctgcgata ggtgtagcag ctcatgaatg
    12601 cggacatgcg gtacaagatg cttcgggtta tgtcccattg aagattcgtg cagcacttgt
    12661 tccagcagct aactttggtt cgatgatttc gttccccttg attatggtag gtgtattgct
    12721 tagctggaac tctacgttaa tcaatatcgg gattttcgct ttttctttag cattactttt
    12781 ccaattggtg acattaccag tcgaatttaa cgcgtctcgg cgagcaatcc aaattttgag
    12841 tgaaggcgga ctactgacgg aagaagaagt accgatggca agacacgtat tatttgcagc
    12901 agcactgact tatgttgcag cagcgctttc gacattcctg cagctgttgc gcttgatctt
    12961 attgttcggc ggcaaccgaa gagattaaaa taaagaagtt gtgaaatcag cgctttgatc
    13021 tgagctaata ccgagagtta gctgattgaa taccatttat ttggaataag aggatgtgac
    13081 aagaattttg tcacatcctc tttgttttgt tctcataacc ttttttcata tacagttcat
    13141 gaaccgcttt tattaacttg ccaaataaca ctgtattgtg cgttgtatcc agatagaaag
    13201 tctccgtttc atctgaaatt ttctcatgct gatctgtaat gatcaaatca gcatgttctg
    13261 aatatgcggt aattttaaca gcatctttgc gaaataactg ctgcaaatgg ctttgaagat
    13321 gataatttcc tgtaaactgt ttggttatct gcaaatggat gagtaactgc tctttcgttt
    13381 ctgtttgtag taagtgttgg atcaattgac taaggtattg actgatttgg tttctttccc
    13441 cattttttga aaaaattgta tcgatttttt ctcttaattt cttttcctgt ataaaagaac
    13501 tagaatgatt ggacgtggct tctggaaagc aaagtgtaga aaaactactg ctggattcac
    13561 ccaacagttc gaaaaaaaca actgccaaca caagaaaata aaaataaatt ggtattttat
    13621 tttctaaatg gttgagagga aactgttgag ttaagtaagt catcaattga ccgacttgtc
    13681 gacaaataag gtttttgctt gtggaaaata aaaaaccaat ctcctgtttt tgtcgaaaag
    13741 agatttgatt tggaaaaaaa caatagaaaa aataattaaa gtaggctctc tctgtttctg
    13801 tctcttcaca agggcgactc actgaaaaag aagtaa
//
LOCUS       DANKKG010000060        13817 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000060 DANKKG010000000
VERSION     DANKKG010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.060"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..222
                     /locus_tag="PW004_001557"
     CDS             <1..222
                     /locus_tag="PW004_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDL2726536.1"
                     /translation="TEHRTIKYLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIR
                     GLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
     gene            643..813
                     /locus_tag="PW004_001558"
     CDS             643..813
                     /locus_tag="PW004_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726537.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            827..1429
                     /locus_tag="PW004_001559"
     CDS             827..1429
                     /locus_tag="PW004_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2726538.1"
                     /translation="MAKIGYARVSSKEQNLDRQLKALEGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVVVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINNLVIELYKYQAESERKRIKERQAQGIEIAKKKGKFKGRQPKFKENDPRLQHAF
                     DLFLNGCSDKEVEEQTGINRRTFRRYRSRYNVTVDQRKKQ"
     gene            1445..2122
                     /locus_tag="PW004_001560"
                     /pseudo
     CDS             1445..2122
                     /locus_tag="PW004_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            2433..2597
                     /locus_tag="PW004_001561"
                     /pseudo
     CDS             2433..2597
                     /locus_tag="PW004_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000898188.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
     gene            2634..2930
                     /locus_tag="PW004_001562"
     CDS             2634..2930
                     /locus_tag="PW004_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
                     /protein_id="HDL2726539.1"
                     /translation="MSKGFLEPALFNQERNVLDSEIKNLTTEKTNLVTNSASGVLRAN
                     EIKDLINYVSADNFNGDYTEELFEEFVVNIIVNSRDELTFNLKCGLSLKEKVVR"
     gene            2931..3347
                     /locus_tag="PW004_001563"
     CDS             2931..3347
                     /locus_tag="PW004_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HDL2726540.1"
                     /translation="MAYIPYGYKIQDGVVTVDEKAAGQVKVFFEKYISGLSLTVAGEQ
                     AGIEKTHSVMGRILKNVNYLGNDTYPAIIDKEIFDKAEEVRDKRAKDLGRVVELAAFT
                     SPPPKERFKMKKADNKMPVDPFERAEYLYSLIESEE"
     gene            3367..4911
                     /locus_tag="PW004_001564"
     CDS             3367..4911
                     /locus_tag="PW004_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2726541.1"
                     /translation="MVIPARKRVGSTAAKEKIKKLRVAAYCRVSTETEEQNSSYEVQV
                     SHYTEFIKKNNEWEFAGIFADDGISGTNTKKRDEFNRMIAECMDGNIDMVITKSISRF
                     ARNTLDCLQYIRQLKDKNISVYFEKENINTTDAKGEVLLTIMASLAQQESQSLSQNVK
                     LGLQYRYQQGKVQVNHKRFMGYTKDEDGNLTIVPEEAEIIKRIYREYLEGQSLVGIGR
                     ALEKDGILTAAGKPRWRPESVKKILQNEKYIGDALLQKTVTVDFLTKKRVKNEGHVPQ
                     YYVENSHEAIIPKELFLQAQEEIHRRSNIYTGEGKNKRIYSSKYALSAITFCGDCGDI
                     YRRVYWNIHGRKEFVWRCVTRIEQGPEVCKNRTVKEDELYGAVMTAINKLLAGGNNMI
                     KTLEENIHAVIGETTEYQISEINNSLEEKQKELIKLANKGQDYDHLADEIDELRDKRQ
                     ILLVEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEGHFVIAFKS
                     GIEMEI"
     gene            5054..5278
                     /locus_tag="PW004_001565"
     CDS             5054..5278
                     /locus_tag="PW004_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2726542.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            5293..6162
                     /locus_tag="PW004_001566"
     CDS             5293..6162
                     /locus_tag="PW004_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HDL2726543.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            6143..6877
                     /locus_tag="PW004_001567"
     CDS             6143..6877
                     /locus_tag="PW004_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL2726544.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            6910..7818
                     /locus_tag="PW004_001568"
     CDS             6910..7818
                     /locus_tag="PW004_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HDL2726545.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            7815..8357
                     /gene="sat4"
                     /locus_tag="PW004_001569"
     CDS             7815..8357
                     /gene="sat4"
                     /locus_tag="PW004_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HDL2726546.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            8450..9244
                     /locus_tag="PW004_001570"
                     /note="aph(3')-IIIa"
     CDS             8450..9244
                     /locus_tag="PW004_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HDL2726547.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            9520..10092
                     /locus_tag="PW004_001571"
     CDS             9520..10092
                     /locus_tag="PW004_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HDL2726548.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIEPISLGFYEQNWYLIGFAG"
     gene            10390..11286
                     /locus_tag="PW004_001572"
     CDS             10390..11286
                     /locus_tag="PW004_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDL2726549.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIIHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMIERIIQLENEKE"
     gene            11385..11594
                     /locus_tag="PW004_001573"
     CDS             11385..11594
                     /locus_tag="PW004_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HDL2726550.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            11611..11883
                     /locus_tag="PW004_001574"
     CDS             11611..11883
                     /locus_tag="PW004_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDL2726551.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            11885..12748
                     /locus_tag="PW004_001575"
     CDS             11885..12748
                     /locus_tag="PW004_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HDL2726552.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(13011..13748)
                     /gene="erm(B)"
                     /locus_tag="PW004_001576"
     CDS             complement(13011..13748)
                     /gene="erm(B)"
                     /locus_tag="PW004_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HDL2726553.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT         4901 a         2156 c         2880 g         3880 t
ORIGIN      
        1 acagaacatc gtaccattaa atacctgaat aatttgattg aacaagacca tcgtccagta
       61 aagagacgca ataaattcta tcgaagttta cgcactgcct ctaccacgat taaaggcatg
      121 gaagccattc gaggattata taagaaaacc cgaaaagaag gcactctctt cgggttttcg
      181 gtctgtactg aaatcaaggt attattggga atcccagctt aaatcataga taccgtaagg
      241 gattttattc tttatttaaa actttgcaac agaacctttt tgagctatgt acaagaagca
      301 ttttattcta gcttaatttg acaaatcata ctatgatacg tgtttgtttt tctgtgctgt
      361 ttagtgaatg attagcagaa atatagagag taagatttta attaattatt agggggagaa
      421 ggagagagta gcccgaaaac ttttagttgg cttggactga acgaagtgag ggaaaggcta
      481 ctaaaacgtc gaggggcagt gagagcgaag cgaacacttg atcttttaag ttgctatctt
      541 tttaaggtca atagagtata cttatttgtc ctaatatggt tttagcagta taattgactt
      601 ggtgaatagg tcatttaagt tgggcataat aggaggagta aaatgaaaaa atttatttat
      661 cgagttttag aaaatgacga agtggtggct atttttaatg agcaacaata tgcgcaagat
      721 tttatcgctt acgaaaagac aatttctgat aagcaatttg aaattgaaaa agtagatata
      781 gctgattggt tattgcaacc gagagaattt tagaggttgg ttgaaaatgg ctaaaattgg
      841 ttatgcacgt gtcagtagca aagaacagaa cttagataga caactgaaag cgttagaggg
      901 cgtttctaag gtcttttcag acaaattaag cggtcaatcg gtcgaacgcc cacaattaca
      961 agctatgctt aactatattc gtgaagggga tatagttgtt gttactgaat tagatcgatt
     1021 aggacgaaat aataaagaat taacagaatt gatgaatcaa attcaaatta agggggcaac
     1081 cctggaagtc ttaaatttac cctcaatgaa tggtattgaa gatgaaaatt taagacggct
     1141 gattaataat ttagtgattg aattgtataa gtaccaagcg gaatctgaac gcaaacgaat
     1201 taaagaacgc caagcccaag gaattgaaat tgctaagaaa aaaggaaaat tcaaagggcg
     1261 acaacctaaa ttcaaagaaa atgatccacg tttacaacac gctttcgatt tatttttgaa
     1321 tggttgttcc gataaagaag ttgaagaaca aactggaatt aatcgccgaa cgtttagaag
     1381 gtatcgatca agatacaacg tgacagtaga tcaaagaaaa aaacaatgaa aagagggata
     1441 gttaatgagt acggttattt tagctgaaaa accaagccag gcattagcct acgcaagtgc
     1501 tttaaaacaa agcaccaaaa aagacggtta ttttgagatc aaagacccac tatttacaga
     1561 tgaaacgttt atcacctttg gttttgggca tttagtcgaa ttagcagaac caggtcatta
     1621 taacgaaaag tggcagaact ggaaacttga atctttgccg atttttcctg atcgatacga
     1681 ttttgaagtt gcaaaagata agggaaagca gtttaaaatt gttgctgaac ttctcaaaaa
     1741 ggcaaataca attattgtcg caacagatag cgacagagaa ggtgaaaata tcgcctggtc
     1801 gattatccat aaagcaaatg ccttttctaa agataaaacg tataaaagat tatggatcaa
     1861 tagcttagaa aaagatgtga tccgaagcgg ttttcaaaat ttgcaaccag gaatgaatta
     1921 ctatcccttt tatcaagaag cgcaaacacg ccaaattgcc gattggttga tcggcatgaa
     1981 cgcaagccct ttatatacct tgaatttaca acaaaaaggc gtacaaggta cattttcatt
     2041 aggacgtgta caaacaccga ctttatatct catttatcaa cgccaggaag caatagaaaa
     2101 ctttaaaaaa gaaccttttt tcctgaataa ttcatagttt tactaatagt ctttcctaac
     2161 attgatttaa tagtctttaa agtattctgc ttcatcaccc attaggtgat gaaaaaagaa
     2221 cccctttctg atttagcgga caaatacttt gacctaatct cagaagatat aaaagaaaaa
     2281 ataataaaat atccgattta tactttgtat aaagaactaa agtagttagc aaatcattta
     2341 cagcaaatca ctatgttcca cataccattt tacatactta ccttatccac gacacatgtt
     2401 gagatagtat cgaagataaa gaaagttgct ccatgttgtt tttgcgagat ggggaaatca
     2461 aagccacatt gacaaccatg atgaataagc ttgctttcag tcataagcta atcctagaac
     2521 cacttttcaa gtcagttagc caaatcgatg aagaaagcga ccgtgaaaga atggacgcta
     2581 ttgataagct aatggagcaa ctattggaag aacgcaatac ccttattgca ctgatgtcca
     2641 aaggtttcct agaacctgct ctttttaatc aggaacgaaa tgttttagat agtgagatta
     2701 aaaatcttac taccgaaaaa acaaacctgg taacgaactc tgcgagtggg gttttgcgag
     2761 caaacgagat aaaggacctc attaattatg tgtcagcaga taattttaat ggtgactaca
     2821 cggaagaact atttgaagaa tttgtagtga acatcattgt aaattccagg gatgagctga
     2881 cattcaattt gaaatgcggt ctttccctga aagaaaaggt ggtgagataa atggcatata
     2941 ttccatatgg atacaaaatt caagatggag tggttactgt cgatgaaaag gcagcaggtc
     3001 aagtaaaggt attctttgag aaatacatat caggactatc ccttacagtg gctggcgaac
     3061 aggcaggtat tgaaaagaca cattctgtga tggggcgcat tttgaaaaac gtcaactacc
     3121 ttggaaatga tacgtatcca gcaattattg ataaagagat atttgataaa gctgaagaag
     3181 ttagagataa acgtgcaaag gatttaggac gagtggtaga gcttgccgct ttcacctctc
     3241 cccctcccaa agaacgattc aaaatgaaaa aggcagataa taagatgcca gtggatcctt
     3301 ttgaacgagc agaatactta tacagtctga tagaaagcga ggaataaagt gacagagaaa
     3361 aatataatgg ttattcctgc tcgtaaaaga gtaggaagta cagccgcaaa agaaaagata
     3421 aagaaacttc gtgttgctgc ctattgccgt gtttctacag aaacagaaga acaaaattct
     3481 agctatgagg ttcaggtctc acactataca gagtttataa agaaaaataa tgaatgggag
     3541 tttgctggca tctttgcaga tgatggtatc tctggtacga acactaaaaa gcgtgacgaa
     3601 tttaatcgca tgattgcaga gtgtatggac ggtaacatcg acatggttat tacaaaatcc
     3661 atcagccgat ttgcgcgtaa caccctagac tgccttcaat acattagaca gctcaaggat
     3721 aagaacatat cagtttattt tgagaaagag aacatcaaca ccacggatgc caaaggtgag
     3781 gttttgctga ctattatggc atctttggca cagcaggaaa gccagagcct ttcacaaaac
     3841 gttaagcttg ggctacagta ccgataccaa caaggaaagg tgcaggtcaa ccataagcga
     3901 tttatgggct acacgaaaga tgaagatgga aatttaacta ttgtccccga agaagctgag
     3961 attatcaaac gcatctaccg agaatacctt gagggtcaga gtctagtggg cattggtcga
     4021 gcccttgaaa aggatggtat tttaacagca gcgggaaaac caagatggcg accagaatca
     4081 gttaagaaga tacttcaaaa cgaaaaatac atcggagatg cccttctgca aaagactgtc
     4141 acagtagatt ttctgaccaa gaaacgagtg aaaaatgaag ggcatgttcc ccaatattat
     4201 gttgaaaata gccatgaagc gattattcct aaagagttat ttttgcaggc gcaagaagaa
     4261 attcatcgga gaagcaatat ctacacagga gaaggcaaga acaaaagaat ttacagtagc
     4321 aagtacgctt taagtgccat caccttctgt ggagattgtg gcgatattta tagacgggtc
     4381 tattggaata tccacggaag aaaagaattt gtctggcgat gcgtgactag aatcgagcaa
     4441 ggtcctgaag tttgtaagaa ccgaaccgta aaagaagatg aactttatgg tgcagtaatg
     4501 actgcgatta ataagctact tgcaggtggg aacaatatga taaagacact ggaagagaat
     4561 attcatgctg tgattggcga aaccacagaa taccaaattt cagagattaa caactcacta
     4621 gaggaaaaac aaaaagaact aatcaagctg gcgaacaagg gtcaagacta tgaccactta
     4681 gcagatgaga ttgatgagct gagagacaag cgacagatcc ttttagtaga agatgcctcc
     4741 ctcagtggcg agaacgagcg aatcaatgag ctgattgaat ttatccgcaa gaacaaattc
     4801 cgcaccttag aatatgatga taagcttgta aggaagataa tccagagcgt tacagtctat
     4861 gaaggccact tcgtcatagc cttcaaatct ggcatcgaaa tggaaatatg aaaaccagaa
     4921 gtaaatagcc catgactcta cagtagagtt gtgggttttc ttttgcttgc tgattataat
     4981 aatattttaa taactattgt gtttatcaac caaatggttt ataatattct tagcgtaaac
     5041 ttggaggtgc tgtatgacta cggcagaaat gattaaagaa ctgtgtgagc aaatgaatat
     5101 aagtgtttcc gaacttgcta gacgtattgg ccagactcca cagaatttca ataaaaaatt
     5161 acaacgagaa acagtaacct tggatgagtt gaaggccatt gctgatgtgc taggtgtcaa
     5221 gtttgtgcag gcatttattt taccagatgg agaagaaata aaaatatcta acgaataaag
     5281 gaggatgttt atatggtagg taatgttatt caaatagtaa cagagaaatt atcatctttg
     5341 ccttttatag aaggcatcgt attagggggc tcacgtgcaa gaggcaccca tacagaaaat
     5401 tctgatatag atatcggaat ctattacaat tcagactcat ttgacctgac agcgattaat
     5461 caaattgcta cagagttgga tgatgagaat agaaacaacc ttgttgtacc tcccggagcg
     5521 tggggtgatt gggttaatgg cggtggatgg ttagttataa acgggtgtca tgttgacttg
     5581 atattacgtg atataaaacg ggtggaacaa ataatcaaag atacggagca aggaattgtt
     5641 actgccaatt atcagaccgg gcatccccat ggttatataa gtgctatgta tcgtggagaa
     5701 ttagcgatta gtaaaatact atatgctaag aacgaaagct tatgcgaatt aaaaaagcag
     5761 gcagaaattt accctactgc tctaaagaaa agcttgatga acttttttat atttgaagct
     5821 gaattctctt taatgtttgt aaaagcaaat gcgggggcag aggataaata ttatattgca
     5881 ggccatgttt ttcgtattat ttcatgctta aatcaagtac tatttgcatg taataatgct
     5941 tattgcatta acgaaaagaa agctataaaa ctgcttgaaa cttttgaata taaacctgaa
     6001 aaatatgccg aaagagttaa tcatattttt gaagtactcg gtctttctct ttttgaatgc
     6061 tacgacatga ccgagaaact ttataaagaa gtgaatgaaa ttgtatcgga gataaataac
     6121 tttttaaacg aggagagttc agatgaaaga aaacaaatat gatgataata tattttttca
     6181 aaaatacagt caaatgagtc gctcgcagaa aggactggct ggtgcgggag aatgggagac
     6241 tttgaaaaag atgctacctg attttaaggg taagcgtgtg cttgatttag gatgcggcta
     6301 tggatggcac tgtatatatg cgatggaaaa cggtgcttcc tctgtagtag gtgttgatat
     6361 ttctcataaa atgctcgaag tagcaaaagg aaaaacccat tttccacaga ttgaatatga
     6421 atgctgtgcc atagaagatg tggatttccc agaggagagc tttgatgtaa tactaagttc
     6481 gcttgcgttt cattatgtag cagactatga gaatttaata aaaaagatat ataggatgct
     6541 gaaggctggt ggcaatttag tttttacagt tgaacatcct gtttttactg ctcatggaac
     6601 acaagactgg tattataacg aaaaaggaga aatactgcat ttcccggtgg acaattatta
     6661 ttatgagggc aaacggacag ctatgttttt ggaagaaaag gttacaaaat atcatagaac
     6721 actgaccaca tatctaaata cactgctttc aaatagtttt ataataaatc agattgtgga
     6781 gccacagccg ccagagaaca tgatggatat tccggggatg gcggatgaaa tgcgacgccc
     6841 aatgatgctg attgtatcgg caaaaaagaa gatgtaataa tatagaaaaa ataaacgagg
     6901 agtatgtaaa tgagatcaga aaaagaaatg atggatttag tactttcttt agcagaacag
     6961 gatgaacgta ttcgaattgt gacccttgag gggtcacgcg caaatattaa tatacctaaa
     7021 gatgaatttc aggattatga tattacatat tttgtaagtg atatagaacc gtttatatct
     7081 aatgatgact ggcttaatca atttgggaat ataataatga tgcaaaagcc ggaggatatg
     7141 gaattattcc cacctgaaga aaagggattt tcctatctta tgctatttga tgattacaat
     7201 aaaattgatc ttaccttatt gcccttggaa gagttagata attacctaaa gggcgataaa
     7261 ttaataaagg ttctaattga taaagattgt agaattaaaa gggacatagt tccgactgat
     7321 atagattatc atgtaagaaa gccaagcgca agggagtatg atgattgctg caatgaattt
     7381 tggaatgtaa caccttatgt tattaaagga ttgtgccgta aggaaatttt atttgctatt
     7441 gatcatttta atcagattgt tcgccatgag ctgctgagaa tgatatcatg gaaggtcggc
     7501 atcgaaacag gctttaaatt aagtgtaggc aagaactata agtttattga aaggtatata
     7561 tccgaggatt tgtgggagaa acttttgtcc acctaccgga tggattccta tgaaaacata
     7621 tgggaagcat tatttctatg ccatcaattg ttcagggcgg tatccggtga ggtggcggaa
     7681 aggcttcatt atgcctatcc ggagtatgat aggaatataa caaaatatac cagggacatg
     7741 tataaaaaat acactggtaa aaccggctgc ctggatagca catatgccgc tgatatagaa
     7801 gagaggcggg aacagtgatt acagaaatga aagcagggca cctgaaagat atcgataaac
     7861 ccagcgaacc atttgaggtg ataggtaaga ttataccgag gtatgaaaac gagaattgga
     7921 cctttacaga attactctat gaagcgccat atttaaaaag ctaccaagac gaagaggatg
     7981 aagaggatga ggaggcagat tgccttgaat atattgacaa tactgataag ataatatatc
     8041 tttactacca agacgataaa tgcgtcggaa aagttaaact gcgaaaaaat tggaaccggt
     8101 acgcttatat agaagatatc gccgtatgta aggatttcag ggggcaaggc ataggcagcg
     8161 cgcttatcaa tatatctata gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg
     8221 aaacccagga caataacctt atagcttgta aattctatca taattgtggt ttcaaaatcg
     8281 gctccgtcga tactatgtta tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct
     8341 ggtatttaag gttttagaat gcaaggaaca gtgaattgga gttcgtcttg ttataattag
     8401 cttcttgggg tatctttaaa tactgtagaa aagaggaagg aaataataaa tggctaaaat
     8461 gagaatatca ccggaattga aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga
     8521 aggaatgtct cctgctaagg tatataagct ggtgggagaa aatgaaaacc tatatttaaa
     8581 aatgacggac agccggtata aagggaccac ctatgatgtg gaacgggaaa aggacatgat
     8641 gctatggctg gaaggaaagc tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg
     8701 ctggagcaat ctgctcatga gtgaggccga tggcgtcctt tgctcggaag agtatgaaga
     8761 tgaacaaagc cctgaaaaga ttatcgagct gtatgcggag tgcatcaggc tctttcactc
     8821 catcgacata tcggattgtc cctatacgaa tagcttagac agccgcttag ccgaattgga
     8881 ttacttactg aataacgatc tggccgatgt ggattgcgaa aactgggaag aagacactcc
     8941 atttaaagat ccgcgcgagc tgtatgattt tttaaagacg gaaaagcccg aagaggaact
     9001 tgtcttttcc cacggcgacc tgggagacag caacatcttt gtgaaagatg gcaaagtaag
     9061 tggctttatt gatcttggga gaagcggcag ggcggacaag tggtatgaca ttgccttctg
     9121 cgtccggtcg atcagggagg atatcgggga agaacagtat gtcgagctat tttttgactt
     9181 actggggatc aagcctgatt gggagaaaat aaaatattat attttactgg atgaattgtt
     9241 ttagtaccta gatttagatg tctaaaaagc tttaactaca agctttttag acatctaatc
     9301 ttttctgaag tacatccgca actgtccata ctctgatgtt ttatatcttt tctaaaagtt
     9361 cgctagatag agttctatat tagagatgta aagagttttg gtgaagaggt ttgtgagtat
     9421 ggcttttata accagggtgc aatgagaagc actaacagca gctagcttaa gaacgcatag
     9481 gatatacttt ttatggaagt cacagaggag gaataaaatt tgaacaggat taacagagta
     9541 acctctattc ttattcagct gcaatcaaaa aaaataattc ctgccaaaga aattgcacag
     9601 cgatttaata taagcttaag gacagtttac agagatatcc ggacacttga agaagccgga
     9661 ataccaattg gatctgaggc cggaaaagga tattttcttg tagagggctt cctacttccg
     9721 ccggtaatgt ttacagcggc ggaagtgggt gcattgatta cagcagggaa atttttaaat
     9781 tgccatggag atgaatcatt tataaaggat tttgattcag ctatgtataa aatcaaatct
     9841 attttgaagc atggtgaaaa aaactatgca caggagttgg agaacagtat taacgtgtac
     9901 agcacatctg gacagaaaaa tacattggcg gataacgtta ttgcagcaat tcagactgca
     9961 atctgcaata aaagggtaat atcaattcaa tatcctgcat caggagggca agaaccggaa
    10021 agcagaatga tagagcctat atcactgggg ttttatgaac agaactggta cttaatcggt
    10081 tttgcaggct aagaaatgaa tataggaatt ttcgtgtgga tcggattaaa agcatttgca
    10141 tagaagaaga gctctgtcaa agccatgatg gctctttgga acaaatatta aaacaaatgc
    10201 tttcatataa aaaactatat gccctacaat gccgttgtaa aaattgggga aaagggatat
    10261 attgatttta tatctttctc aaaataaata taaaagccct ttaaagaggg cttttatata
    10321 ttaatcacaa atcacttatc acaaatcaca agtgattaat cacaaatcac ttgtgatttg
    10381 tgattcttaa tgatacaata ttactataca aaaaaagaat ggggcgtagt tatggagaag
    10441 gaaaaactaa aaatacttga agaattaaga cgtattttaa acaataaaaa tgaagcaatt
    10501 attatcttga ataattactt taaaggtggt gttggaaagt ccaaattatc gactatgttt
    10561 gcttacttga cagacaaatt gaatttaaaa gttttaatga tcgataagga cttacaggca
    10621 acattgacaa aagacttagc aaaaactttt gaggtagaat tgccacgtgt caatttttat
    10681 gaaggcttga aaaatggaaa cttggcttct tctattattc atttgactga taatttagac
    10741 ttgatccctg gcacgtttga tttgatgtta ctgccaaaat taactcgctc atggacgttt
    10801 gaaaatgaaa gtagattgct tgctactctt ttagcacctt taaaaagtga ctatgatctt
    10861 attattattg atactgtacc aacgccaagc gtttatacaa ataatgcaat cgtggcgagt
    10921 gattacgtta tgatcccttt acaagcagaa gaagaaagta caaacaacat tcaaaactat
    10981 atttcctatt tgattgattt gcaagaacag tttaaccctg gactagatat gattggtttt
    11041 gttccttatt tagttgatac ggacagcgca acgataaaat caaacctgga agaactgtac
    11101 aaagaacata aagaggataa tttggttttc cgaaatatta tcaagcgaag taataaagta
    11161 agtacttggt ctaaaaatgg cattacagaa cacaaaggct atgacaaaaa agttttgtct
    11221 atgtatgaga acgtattttt tgaaatgatt gagcgaatca ttcaattaga aaatgaaaaa
    11281 gaatagaatc acaaatcaca agtgattaat cacaaatcac ttgtgatttg tgataggtga
    11341 tgataaaata aatagtaaga agaaatagaa agaagtgagc gatcgtggga aatttaggcg
    11401 cacagaaagc aaaacgaaat gatacgccaa tcagcgcaaa aaaagatata atgggggata
    11461 agacggttcg tgttcgtgcc gacttgcacc atatcataaa aatcgaaaca gcaaagaatg
    11521 gcggaaacgt aaaagaagtt atggatcaag ccttagaaga atatatacgg aaatatttac
    11581 ctgacaaact ttaaaaggag tgaaaatgaa atggcagtta cgtatgaaaa aacatttgaa
    11641 attgaaatca ttaatgagtt atcggcaagt gtttataatc gggtattaaa ttatgttttg
    11701 aatcatgaac tagatactaa aaatactcgt ttactagaag tgaatctttt aaatcaatta
    11761 gaagtggcac aagaagttga tttatttcaa caaccatttg aagaattaca agctattcat
    11821 gagtattggc ggtcaatgaa tcaatattca aaacaaattt tgaataaaga gaaagtggct
    11881 taacatggcg aatattactg acttcaccga aaagcaattt gaagatcgtt tagaaaagaa
    11941 tgttgaacga ctaactaaaa atagactagc ggttgaatcg ccaaccgctt ttttacttgg
    12001 tgggcaacca gggtcaggga aaaccagttt gcgatcagca atttttgaag aaacacaagg
    12061 gaatgttatt gtcattgata atgatacctt taaacaacag cacccaaatt ttgacgaatt
    12121 agtgaaactc tatgaaaaag acgtagtaaa acacgttacc ccttattcga atcgcatgac
    12181 agaagcactt attagccgtt taagcgatca aggctataat ttagtgattg aaggaacagg
    12241 acgaacaaca gacgttccca ttcaaacagc cacaatgctt caatctaaag gttatgaaac
    12301 aaaaatatac gtcatggcag taccgaaaat tgaatcatac ttaggaacga ttgaacgata
    12361 tgaaaccatg tatgcagatg atccaatgac agctagggca acaccaaaac aagcgcatga
    12421 tattgttgtc aaaaacttac cgaccaattt agaaaccctt cataaaacgg gcttatttag
    12481 cgatataagg ctttacaata gagaaggagt gaaactctat tcaagcttag aaacgccttc
    12541 cattagtccg aaagagacct tagaaagaga attaaatcgt aaagtatcag ggaaagaaat
    12601 tcaaccgact ttggagagaa tagagcaaaa aatggttcaa aatcaacacc aagaaacccc
    12661 tgaatttaaa gcaattcaac aaaaaatgga aagcttgcag ctacctacac caccaatacc
    12721 caaaacacct aaacttccag gtatttaaac cttaaaaaag gaaaagagta gttaccaaaa
    12781 aacggtaact actctttttt tataaaaaca tttcctcatg ttggggtccc gagcgcctac
    12841 gaggaatttg tatcgataag aaatagattt aaaaatttcg ctgttttttt ctaatttcat
    12901 tttgaggtga attaattggt agttattaga ggtgccctat acctaataat ttatctacat
    12961 tccctttagt aacgtgtaac tttccaaatt tacaaaagcg actcatagaa ttatttcctc
    13021 ccgttaaata atagataact attaaaaata gacaatactt gctcataagt aacggtactt
    13081 aaattgttta ctttggcgtg tttcattgct tgatgaaact gatttttagt aaacagttga
    13141 cgatattctc gattgaccca ttttgaaaca aagtacgtat atagcttcca atatttatct
    13201 ggaacatctg tggtatggcg ggtaagtttt attaagacac tgtttacttt tggtttagga
    13261 tgaaagcatt ccgctggcag cttaagcaat tgctgaatcg agacttgagt gtgcaagagc
    13321 aaccctagtg ttcggtgaat atccaaggta cgcttgtaga atccttcttc aacaatcaga
    13381 tagatgtcag acgcatggct ttcaaaaacc acttttttaa taatttgtgt gcttaaatgg
    13441 taaggaatat tcccaacaat tttatacctc tgtttgttag ggaattgaaa ctgtagaata
    13501 tcttggtgaa ttaaagtgac acgagtgttc agttttaatt tttctgacga taagttgaat
    13561 agatgactgt ctaattcaat agacgttacc tgtttactta ttttagccag tttcgtcgtt
    13621 aaatgccctt tacctgttcc aatttcgtaa acggtatcgg tttcttttaa attcaattgt
    13681 tttattattt ggttgagtac tttttcactc gttaaaaagt tttgagaata ttttatattt
    13741 ttgttcatgt aatcactcct gaagtgatta catctataaa taaatacaga agttaaacga
    13801 tttgtttgta attttag
//
LOCUS       DANKKG010000061        13782 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000061 DANKKG010000000
VERSION     DANKKG010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13782
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.061"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(<1..187)
                     /locus_tag="PW004_001577"
     CDS             complement(<1..187)
                     /locus_tag="PW004_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726554.1"
                     /translation="MTIELEEHLLWFKYDPSEKFVKDFYKVWDSEVFFLAIEDSLLIN
                     LYYSNKNYFKIPAAKTRM"
     gene            complement(714..786)
                     /locus_tag="PW004_001578"
     tRNA            complement(714..786)
                     /locus_tag="PW004_001578"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(751..753),aa:Lys,seq:ctt)
     gene            1167..1736
                     /locus_tag="PW004_001579"
     CDS             1167..1736
                     /locus_tag="PW004_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HDL2726555.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1723..2583
                     /locus_tag="PW004_001580"
     CDS             1723..2583
                     /locus_tag="PW004_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDL2726556.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2773..3477
                     /locus_tag="PW004_001581"
     CDS             2773..3477
                     /locus_tag="PW004_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2726557.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            3482..5317
                     /gene="walK"
                     /locus_tag="PW004_001582"
     CDS             3482..5317
                     /gene="walK"
                     /locus_tag="PW004_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HDL2726558.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            5314..6630
                     /locus_tag="PW004_001583"
     CDS             5314..6630
                     /locus_tag="PW004_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726559.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            6631..7500
                     /locus_tag="PW004_001584"
     CDS             6631..7500
                     /locus_tag="PW004_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HDL2726560.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            7555..8364
                     /locus_tag="PW004_001585"
     CDS             7555..8364
                     /locus_tag="PW004_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL2726561.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(8467..9096)
                     /locus_tag="PW004_001586"
     CDS             complement(8467..9096)
                     /locus_tag="PW004_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HDL2726562.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            9266..10558
                     /locus_tag="PW004_001587"
     CDS             9266..10558
                     /locus_tag="PW004_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2726563.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(10634..11296)
                     /locus_tag="PW004_001588"
     CDS             complement(10634..11296)
                     /locus_tag="PW004_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDL2726564.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            11521..11805
                     /gene="groES"
                     /locus_tag="PW004_001589"
     CDS             11521..11805
                     /gene="groES"
                     /locus_tag="PW004_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDL2726565.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11856..13481
                     /gene="groL"
                     /locus_tag="PW004_001590"
     CDS             11856..13481
                     /gene="groL"
                     /locus_tag="PW004_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDL2726566.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4616 a         2357 c         2933 g         3876 t
ORIGIN      
        1 tcattctagt tttcgcagca ggaattttga aatagttctt attagaatag tagagattga
       61 ttaataagct atcttcgatt gctaaaaaga aaacttctga atcccacacc ttataaaaat
      121 ctttgacaaa tttttctgat ggatcgtatt taaaccaaag cagatgttcc tctagctcga
      181 ttgtcatgtt tttcacctca acgaaagtat acgaactaat gtttgttttg taaaggataa
      241 aaatagggta gcaaccggta cccaaactag tacccattta tcaaaaacga cgaaatttat
      301 gtaaatagaa aaataagaat aaacattgat gtatcaacat ttatatatct ttagaacaaa
      361 tgaaaagggt ggttctcata agaaaaacat gttcgcactt ctaggcaaac caggatttga
      421 agaccttgct aaagaattga acgaacgcct gtaaaataag gatttgtcgc tagatttaaa
      481 aaatcagtga tagaaacatg tttgctttaa aatgaaacta gtgatagttt tagtgatagt
      541 gaacttaaaa aaagcactca atttgagtgc ttttttattg ttattaaaaa ttaaaaaact
      601 aaaatacata cccgaatttc atacccgaaa tataaaaaat gtcatcacgt acatcttttt
      661 cgaaactacc gaagtagcca aagatgtact gatgacgtgg gtttggcacc caatgggccg
      721 tgaggggctc gaacccgcga cccgctgatt aagagtcagc tgctctacca actgagctaa
      781 cggcccgtac aaaaagtact taataatagt agcactaaga attttaaaat gcaagactac
      841 atttaaagaa aatcagaaaa atctttactt ccttatagga aatcattcat agattattca
      901 catttgctcg ctattatata agcataccaa caaacaaagt tcttgcaaga gaacaaaccc
      961 taacactttt ttcatttttt actcctttcc cgccgtctcc ccggcgggtc tcctgttctt
     1021 aacgataaaa gacaagaagg cttagccttc ttgttttttg ttttcatgga atcccgctaa
     1081 tcaaatcacc tagttaaagc aactagattg aatgtttatc ataagtctga tatcatgttt
     1141 atgaaatgaa aaaaggagcg gttatgatgg aaaaaatgcg tcagcaatta atggagtggg
     1201 ggaaggaact agaaacattc cgtctgcctc actgggaaga actccctgac ttagaacttt
     1261 atatggacca agtcagaacg ttagttgatc gttatctttc acctgtgata caaggagaaa
     1321 agcatccatt gcttacctct tctatggtaa acaattacgt taaactaggt ttgataccag
     1381 caccagttaa aaaaagatat aacaaagaac acgtagcatt tttacttgca atcactacgt
     1441 tgaaacaagt attgactatt cctgaaatca aagaagggat attgttccaa ggaaaaacag
     1501 taggaattcg tgaagcctat aatctatttt gcgatgaaca agaagcagct gtgtggatgg
     1561 tcagccaatt agcacaaggt aaatctcatc cgcaaaaatt cgaccaggca acccctgtag
     1621 aatatattgc tgtaagagcg gcaactttat cgtttgcaat gaaacttctt gcggaaaaaa
     1681 cgattgttct agaaaccgaa tatttaaaag aggagaaaac aaatgaaaaa caataaaatc
     1741 gcaattttag tagattcagg tacggatgtg ccagaagaat tgataaaaaa atacgagatg
     1801 tatatgatcc cattgaaaat tatttataaa gatcaggtat atacagataa aattgacatt
     1861 acctctgaag agatctatca acgtttgcca gaggaaatcc ctggtacatc tttgccggat
     1921 ggtgagtcga tcacagaaat ctttgagcaa atcaaaaaag acggttatga aaaggtactt
     1981 gcggtaacaa tttccagtgg attgagcggg acatataatg tggttcgttt aattgcacaa
     2041 cagcaagaag aattggaagc atatgtacta gatacgaaaa atattggtat tggtgcaggt
     2101 ttttctgcga tccaagcagc aaaatggctg gaagaaggag tagaatggga tcaactgatc
     2161 agtaatctga atgagttagt cgaaagaaca aaagttttct ttaatgttgc gacattggaa
     2221 tatcttcaaa aaggtggacg gatcggttta gttgcttcta ttgtaggaac cgctttaaag
     2281 cttaacccaa tcatctcatg taatgaagaa gggatttatt acacagtagg aaaagctagg
     2341 ggacgtaaaa aaagtttgga taaaacggtt gcttacgtca aagaaaaaat cggtaatgca
     2401 aaggtcttca acttagctgt tgcccatggg gatgcgaaag aagaagcaat tgaaatggaa
     2461 gcgagactta aaagagagtt cccacaagca aaccaaatct actttggcca gatttctccg
     2521 gcattagtag tccacactgg tccgggatta ttgggtgtcg gtgtacaagt tttagaagga
     2581 taaatcaaaa aaaggaaaca ctgaggtgaa aatcagtgtt ttcctttttt attgaagtta
     2641 gaacaaaaaa taataaagta ttttgcactt tcattttaat tttctctttt gttttatagg
     2701 caaagatgaa acaaaagcag aaatacggta taatgagaag gtatgacaat aatatataag
     2761 aggtggctct ttatgaaaaa agttttagtt gtcgatgatg agaagccgat ttcggatatc
     2821 gtcaaattca atttagccaa agaaggctat gatgtttata ctgcatatga tggggaagaa
     2881 gccttggaaa aagttgctga ggtagaacct gatttgattt tacttgattt gatgctacct
     2941 aaaatggacg gcttagaagt tgctcgtgag gtacgcaaaa cttatgatat gccgatcatt
     3001 atggtaacag ccaaagattc tgaaattgat aaagtcctag gcttagagtt aggcgccgat
     3061 gattacgtga ccaagccatt ttccaaccga gaattagttg cacgtgtcaa agcaaatctt
     3121 cgtcgcggtg ccacggctgc gaaggaacca gaagaggcag cacctgcgga attgacgatt
     3181 ggtgatttga cgatccatcc agaagcttac atggtgacga aacgaggaga aacaatcgag
     3241 ttaacacatc gtgaattcga actactcttt tacttggcga agcaccttgg acaagtgatg
     3301 acaagagaac atttattaca aacggtttgg ggttatgatt attttggaga tgtacgaaca
     3361 gtggatgtaa ctgtacgtcg tttgcgtgaa aagatcgaag ataatccaag ccatccaaat
     3421 tatttagtga cacgtcgtgg tgtcggttac tatttgcgta acccagaaca ggagtaattg
     3481 gatgaaaaga aaagtccgct tttttcgatc agtcaatttt aagatcgcta ttacctttat
     3541 tctgatttta ttgatctcga tcgaaattat tggtgcctac tttatacgtg gtttagaacg
     3601 ttccacgatc aatacattta ttaaagatat gaatcaaacg gtggaatcat tagcaacaac
     3661 aatcagcccg gaattgaatc gaaaagataa tgcagatgat gaagaggtaa atgcgaatat
     3721 caaaagattt attgaaaaca gtgcgactag cgatatcatc gaaatacggg tcgttgacga
     3781 aaaagggatc atccgaggaa caaccgatgt caatgaacaa agcgctgtgg gaaagaaaaa
     3841 tgattatgtt gatatcaatg atttcacgac taaacgttat gttgctttag ataacgacga
     3901 taaacgggtg aatatcaatg ttcaaccgat cctgtccccg accggcgatg ccgttatcgg
     3961 ggctctttac gtcaaaagta atatcgaaca aaaatattcg gaaatcaata acacagcagt
     4021 cattttcttt actgcctcac taattgcggc atttatttca atgatcgtat ctgttttagt
     4081 tgctcgttcg attacacagc cgatcggtga aatgagagaa caagccattc gaatcgctag
     4141 aggagactac agtcggaaag tcaaagtgca tggacaagat gaacttggac agctggctga
     4201 taccttcaat cagttagctg aacgtattga agaagctcaa gatacaatgg aagctgaacg
     4261 caatcgtttg gatagcgtgc tatcccatat gacagacggc gtcattgcga cagatcgcag
     4321 gggtaaagtc attacgatca atgatatggc tgtttccttg ctagatatca aaaacgagga
     4381 agcaatcgga caatcgattt tgacactttt agatatcgaa gaagaatata cgttgcggaa
     4441 attattagaa gatccaaacg aaatggtact tgaccgttca accagtacat tggaaagtga
     4501 tcagatgatc ttgcgagtag acttttctat gatccgtcgc gaatcaggtt ttatcagcgg
     4561 attagtagct gtcttgcatg atgtgacaga acaagaaaaa acagaacggg aacgtcgtga
     4621 attcgtctct aatgtttccc atgaacttcg tacaccgttg acaagtatgc gtagttatat
     4681 cgaagcattg agcgaaggtg cgtggaaaga cgaggaagta gcaccaaatt ttttgaaggt
     4741 aacgctagat gaaacagacc gaatgattcg aatgatcaat gacctcttaa atctatctcg
     4801 catggataca ggtaataccc agcttcaatt agaatatgtc aatttcaacg agttagtgaa
     4861 ttttgttctt gaccggtttg atatgatggt gggaaatcag gaaaaaaatt ataagatccg
     4921 tcgtgaattt acgcaaagag atctttgggt cgaagtagat acagataaaa tcatccaagt
     4981 agtcgataat atcatgaata acgcgatcaa gtattcacca gatgggggaa cgattacttg
     5041 tcgcttatta gaaacgcata ataacgtggt tttaagtatc accgatcaag gattggggat
     5101 tcctaaaaaa gatctgaacc gagtgttcga gcggttctat cgagtagata aagcacgtgc
     5161 aagagcacaa ggtgggacag gacttggatt agctatttct agagaagtga ttaaggcgca
     5221 tcgaggcgct atttgggcag agagcaagga aggaaaaggg tcgacttttt atatctcgct
     5281 gccatatgaa ccttatgagg aggaatggtg ggaatgagaa tctctgaaaa agtcgttcgt
     5341 ataggactca ttttgatggt tgcgttaagc atatacttct cttatgcaat ctggctgagc
     5401 ccagcaggaa aaagttcaat caacgtagat gagacaaaca ctcaaatgat cgagtctcaa
     5461 agcaatcgaa aaacttccga agtctttctt ccgcttcatg ttacatggca ccattcggga
     5521 atgatccaag aaaccaacag tgaaaatctc gtgtaccgat tgcaattgat catcgataaa
     5581 gcaagatttg gaaagctttc tgaagtagtt tcaggagaca aatcacagtt tgaacaggtg
     5641 aaaaatctga actctggttt tgagttggcc tataatgcgc cttttttgct aagagaatac
     5701 aaggaagcat ttgatttaga tttagatgtg tcgaacttgg caaaagaaag cgaaaatatg
     5761 tattttacta gaatacaatt cgatcaagga aataaaaaaa tccgattttt gaattattcg
     5821 aaccatctcg tatatgaagc aaatatttcg ttgaattgga cagatatcaa taaagaatta
     5881 acagcaaaag acacaaaatg gacagaaatg acaacagaac catcgacgat cgacacgcaa
     5941 tatgctacga aacattcgat caaactgaaa aagtatagtt atatcctgtc acagcaaccc
     6001 tatacacttt ttagaaatgc ttttttccag aaaccagata gcgtgaaaaa taatgatgaa
     6061 agcaatgaat tgatttttta cggtgggaat gaaacaatgt ctatccattc ggaaacgcaa
     6121 gtagttgatt ttcgtggaga gctgccagag aaaaaagaaa atgctagtgt attttctgaa
     6181 agtttcccct atattagcaa attaggcagt tctttaggga acctcagata ttttgaccgc
     6241 agtgacaata cgatcgatta tcggatattc gtggaaggct tcccggtttt tgggacagac
     6301 agcagaggac agattggtgt ggaagttggc ggtgaaacga atggttctgt gcaagtgaat
     6361 atccaaacca gtttgaatac gattcaagtg ccgatacctt cagacgaaga agtagaactt
     6421 gcaagtacga atgaaatcat ccagcagtta aaagctaagg gagcagatag caacaagatt
     6481 ggttcagtga tcgttggcta tacatggcaa aatgtcaaag aaacgaatcg tgtcgttgat
     6541 ctcttacctg aatggtatgt gaaatataaa gataactggt attcagctaa cgaccttctg
     6601 gaaaaattac ctggattgga ggcgaattaa ttggatttca aaaaaatcga atggatattt
     6661 ttcgtcgctt ttttagggtt gaatgtgttt ttattcagca gttatcatga cgcggtatac
     6721 caagaaaaca atgtcattcg ctctaatcag actgaatcaa tccaacaacg tctggctagc
     6781 gacgatatca cttacacggg gaagatttcg tctgagaaca agcaaggcta ttatcttagc
     6841 gcggagcaga ccaatcttag cacttcatta gcgaattatc gcaaggctgg taaaagcggc
     6901 ttgctggcaa gtggtgtgtt tttagacggt gatgttttga cgaaatcttt atccagttca
     6961 gatgctgaaa agaacgtgat cgatccagat aaaattagta gttccttgga agatttttta
     7021 tctaacaaag acaatatttt attcggtaat gattatgtgt acacgaaaaa cttttctcat
     7081 ttagaagaag aaccactaga aatccaagca agccaagagt atgaaggcat cccattcaat
     7141 gatgatacag caaaaatcgt tctttcttta gaaaaaaacg ataatgatta tagcatcacg
     7201 aaatacatgc agacgcattt atctgatatc gagcagctgc gcgaaaagac ggaactgtat
     7261 acggaagagg aagcaatcaa tacattgtac atcaataaca aaataccaag aggatcaaaa
     7321 attttatggc gtcagctagc ttattcctgt attctgaaag tccgggaaaa aaatgtgtat
     7381 gttcctgttt ggcatgtagc gatcgaaaca agcgataaag tggtccaaat tgagagtgtc
     7441 aacgctttca gcaatacgat cgtgacaaac aatactgtac caaaagtgga agatcaataa
     7501 aaaagcgagt ataatgaaaa aagtttacaa agtaagaagg gaagtaggag tcaaatggct
     7561 gaagaaaaac cagcttttaa aatcagtatc ctagcaagtg gaagttcggg taattcactt
     7621 tatatcgaaa gtgaaaaaaa acggcttttg gtcgatgcag gacttagcgg taaaaaaatc
     7681 acttccttga tggctcaaat tgatcggaca ccggaccaat tagatggaat acttgtgacg
     7741 catgaacatc gagatcatat acatggcgtg ggtgtcttgg ctcgtaagta tcatttggat
     7801 gtttttgcca atcagcagac ttgggaagcg atggaaccac tgattggaaa tgtaccagtg
     7861 gaacaaaaac atctatttga gatggggaaa gtcagaacat tcggcgatat cgatattgag
     7921 agtttcggcg tctcccatga cgcagcggct ccgcaatttt atcgtttcca taaagaaaat
     7981 cggtcattcg tcatgttgac ggacaccgga tattgcagtg attatatgcg aggtgtcatt
     8041 gaaaatgccg atggttattt gatggaaagt aatcatgatc ttgagatgtt acgcatggga
     8101 ccttatccgt ggaatttaaa acagcgtatt ttaggagata gaggacatct ttccaacgaa
     8161 gacggtgcac ttgtcatgac tgatgtgatc ggtaatcgga ccaaacgaat ctatcttggc
     8221 catcttagca aggaaaacaa tatgaaagaa cttgctcatt taacaatgga aaatgttttg
     8281 aaagaaaaag attttggcgt tggacatgat tttgaaatct atgatacaga tccagatgaa
     8341 gcaactgagc tattttcgat ttaaacagtt caatcaataa aaatataaaa agcagaagat
     8401 aaaatcaatc agggttggtt ttatcttcta tttttatttt tttagcatat taaaaaaata
     8461 accgtattat cgactgacta cactgatgtg ctgcttttca tgtttctcat ccagtatctc
     8521 atctactaat gcaatcgcgt aatctgccat gctgatcgta ctgtcgccag cagcgtttct
     8581 ttgtaaataa tcatcactta acaaataagt acctgtacgt ttgccatgtg gatcaaataa
     8641 agaagctgga ctgatataag tccaatgtag atttttagat tgtttcaatt ttaacaatcc
     8701 ttcgtacata ttgaaagctg ttggatagaa gggtgcattt ttaggtactt ggtcaatcat
     8761 acgagtcgtc ttttctgtat ctaagaataa cgaagaggca cccccaacga caattaagcg
     8821 tgtatcggtt ccttcaagca tctcaatcaa atgggacaaa gaagtttgat gcatttcttc
     8881 ttttcctttg ggagcgttga aagcatcaac gacaacatca aaagatgcaa gatcagtagt
     8941 tgtcaactca aataaatcct tttgtataaa cggaacatct gatggcaatt tttctggatg
     9001 acgcacgatt gccgtcacgt ctaaatctct ggacaatgct tcgtctaaaa taaatgaacc
     9061 tgcatggcca gtagctccaa tgagtgcaat tttcataaaa aacctccttt tttattcctg
     9121 tccattgtaa gcagttgctg aatattcgtc aaagaatccg atttttgaaa agctcagtgc
     9181 gcattccttt catggttcgt gatcattggg tatagttaaa ttaagaaaac tgattgataa
     9241 aaatagaagt tgaaggagat aagaaatgaa agagtggtta gatcgtgcct ttttaccaat
     9301 gataacatct gttgtgaatc ttagaccctt aactgctttg cgtaatggaa tgacttacac
     9361 gatcccaata attattgttg gctcgctgtt tttcttattt gctcattttc ctgtggatta
     9421 catcgctcag tggatagaca aaacaggatt ttctatgtat tttacacaag tataccaggc
     9481 tacatttgat gtgatagcct tttggatcgt ttttgccatt gcatacgctt atgcaaaaga
     9541 agaagacatc gatcgcttac cagtcggcat aacagccttg tctgcttatc tactgcttat
     9601 tcagccacta aatactcaga tagatactgt ctggacaggt gctgaaggga tactaggagc
     9661 agttacaact gggctcatca tcggatgggg ctatgcacgt ttagtaagac ttgcgaaagg
     9721 gtggaagcaa agagagaaga tacctgaaaa tgtttggcag tcttttactt cattgattcc
     9781 aatcattgtt atagtcactt ttacgcttat cggttcagcg gtttttcaac agttgttcag
     9841 aatgactgtt gttcaaggag tatggacata tgtccagctt cccattcaat cgttcataga
     9901 cacgttgttt ggtgtgcttt tattaggttt ttttgttccg tttttatggt tatttggtct
     9961 tcatggatct gcgatggtca atgggttggt tagtcccgtc ttgcaagcga attcattagc
    10021 gaatgcagaa atcttagcct caggacaaga gttgacggtg gcaaatggcg gacatatcgt
    10081 tactcagcaa tttctcgatc agttcatgac ggttacgggt gctggattga cgttaggggc
    10141 tgtatttttt atgacggttt ttgcgaaatc gaagaaatac agagaattag gaaatcttgc
    10201 tcttttacca gcctttttca acatcaatga atcaattatt ttttccacac cgatcgtgat
    10261 gaacccgatg atggctgttc cttttatttt cgcaccggtc gtttcaggat tgattaccta
    10321 ttccgcttta tattttggat tcgttccttt atttacagca gtccaagtcc cttggacaac
    10381 accgccgatc ctctcgggtt tcttagtagg cggagtgcct gcagcgattt tacaaggtat
    10441 cgtgttgtca attagttttt tcatttattt cccatttttg aaaaaaatag attctgcaaa
    10501 ctgcaaaaag gaaagacaga caaaaaatga tggagcagca gaaaagatga acgactaaaa
    10561 aacaaaaata ctcctgtcgc tttagattgg gaaaatcacc aatctaggat gacaggagta
    10621 tttatagtca tgtttatact agcccttttt ggacagctaa ttgaacaaga acaactaatg
    10681 tgttcattcc tacgtgtgtg atcatcgatg tccagatccg accagttgct ttgtactcta
    10741 ggctgaagaa gaaacctagg aaaaagtaga ttagcagatg accatcatta tgagcaaacg
    10801 caaacagcaa agagctgatc actgctccga cccaaaaatt tgaatatcga ctgatgatcc
    10861 ccagtatact gcggcggaat acgaactctt ccatgatggg tccagcaaca gttgtcgcca
    10921 aaataaaagc gggtgcttcc atgatcatct ggatgatatt ttgtgtattc tcggaagggg
    10981 ttgctttgcc aaacaataca gattctaaca tgacagcgat gctttgcaga atcaatgcga
    11041 caaagattcc aaccaatcca taaataacaa tctttggagc agaagctttt tttaccgctg
    11101 attcgatcgc taagggttca cgttgcttaa aataaaaaat agctaggaca acggcgccta
    11161 gtatatacat gactgcagtt gctgtgatca attcagacgt agattttaca agtcctatcg
    11221 aagctaggat caagggtgag ataaaaatta gaccatagca aaaaatgctg aggaagctat
    11281 atttttttaa agacatgtaa ttcctccttt taagggataa caagcatgca aggcaagctt
    11341 atatcgattg atttcaatta tatcataatt tcaataagca gctgtcagaa taaaatttct
    11401 tttctttgat ttaaaagctt gcaaaaaaga gggagaatga gtattattaa aaatgtaggt
    11461 tagcactcga aggttaagag tgctaacaga ttaaaaaaag agttatcgga gggatttatc
    11521 gtgttaaaac cattaggtga tcgcgtcatt atcgaagtcg caaaagaaga agaaaaaact
    11581 gtcggaggaa tcgttttagc ttctgctgct aaagagaaac cccaaactgg gacagttgtt
    11641 gcagtcggtg aaggccgttt gttagaaaac ggtgaaaaag ttcctgccgc tgtaaaagca
    11701 ggcgaccaag ttatgttcga aaaatacgcc ggtacagaag taaaatacga aggaaaagaa
    11761 tatctgatcg tcgcaggaaa agatatcatg gcgattgttg agtaaatttg gaagataaaa
    11821 aaataatcaa aaataatcaa tgaggtgaat caatcatggc aaaagaattg aaatttgcag
    11881 aagacgcacg cgcagctatg ttacgcgggg tagataaatt agcagataca gtgaaagtaa
    11941 cattaggacc taaaggccga aatgtcgtac tagaaaaatc ttacggctct ccattgatca
    12001 caaatgatgg tgtgacgatc gcaaaagaaa tcgaattaga agatcatttt gaaaacatgg
    12061 gtgcaaaact tgtatcagaa gtagcatcaa aaacaaatga tattgcagga gacggtacaa
    12121 cgacagcaac tgttctgaca caagcaatcg ttcgcgaagg tttaaaaaac gtaactgcag
    12181 gtgctaatcc tctaggcatt cgtcgcggga tcgaattggc aacaaaagca gcagtcgaag
    12241 aattgcataa tatctcaact gttgttgatt caaaagaagc cattgcacaa gtagcagctg
    12301 tttcttcggg ttctgataaa gttggacatt tgattgctga tgcaatggaa aaagttggaa
    12361 acgacggtgt catcacaatc gaagaatcaa aagggatcga aacagaacta gatgttgtag
    12421 aaggtatgca gtttgatcgt ggttacttat cacaatatat ggtaacagat aacgacaaaa
    12481 tggaagctgt tctagaaaac ccatatatct tgatcacaga caaaaaaatc tctaacatcc
    12541 aagatattct gccattgtta gaacaaatct tgcaacaatc acgtccattg ttgattattg
    12601 ctgacgatgt agatggtgaa gcattgccaa ctcttgtatt gaacaaaatc cgtggaacat
    12661 tcaacgtagt agctgtaaaa gctcctggtt tcggcgatcg tcgtaaagca atgcttgaag
    12721 atatcgctat tttgacaggt ggtacagtaa ttactgacga tctaggttta gaattgaaag
    12781 atgtaacaat cgaaaactta ggaaatgctt ctaaagttgt tgtagataaa gacaatacaa
    12841 cgatcgtaga aggttctgga gaaaaagaag caatcgaagc tcgtgtgcaa ttgatcaaaa
    12901 accaaattgc tgaaacaact tctgattttg accgcgaaaa attacaagaa cgcttagcta
    12961 aattagcagg tggtgtagct gttgttaaag ttggtgcagc tactgaaaca gaattgaaag
    13021 aattaaaatt acgcattgaa gatgcattga atgctacacg tgccgcagta gaagaaggaa
    13081 tggtctcagg tggtggtaca gctctagtga acgttatcag caaagtttct gctgtagaag
    13141 cagaaggaga cgtggcaaca ggtatcaaga tcgttgttcg tgctttagaa gaaccaattc
    13201 gtcaaatcgc tgaaaacgct ggttatgaag gatcagtgat tgttgataaa ttgaagaacg
    13261 tggaactagg cactggtttc aatgctgcta ctggagaatg ggtaaacatg gttgaagcag
    13321 gtatcgtaga cccaactaaa gtaacacgtt ctgccttgca aaatgccgct tccgtttctg
    13381 ccttgttatt aacgacagaa gcagtcgttg cagataaacc agaaccagca gcgccagcag
    13441 ctcctgcaat ggatccatca atgatgggcg gtatgatgta agaaaaagca acttgcttat
    13501 ctacaactga tcattagtga aagacagata aaaagaggct ggaacagaag cgtttaactc
    13561 caagaaataa gaagaaattc acggaaattg cttttcaaat ttttgtgaat ttcagcttat
    13621 ttccgaagga gttgcttctg cttccgctgt ttatacgtgt ttagagcgtg agacaaaagt
    13681 gttctttact tttgtcccac gctctttttt gtgtgtaatt ataatgaaaa tagttatttt
    13741 taatagttat aatattttat ctattaaaag taaatatcca tt
//
LOCUS       DANKKG010000062        13615 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000062 DANKKG010000000
VERSION     DANKKG010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.062"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(231..923)
                     /locus_tag="PW004_001591"
     CDS             complement(231..923)
                     /locus_tag="PW004_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDL2726567.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            complement(1360..1929)
                     /locus_tag="PW004_001592"
     CDS             complement(1360..1929)
                     /locus_tag="PW004_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDL2726568.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(2003..2326)
                     /locus_tag="PW004_001593"
     CDS             complement(2003..2326)
                     /locus_tag="PW004_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726569.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2480..3292)
                     /locus_tag="PW004_001594"
     CDS             complement(2480..3292)
                     /locus_tag="PW004_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726570.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3646..4494
                     /locus_tag="PW004_001595"
     CDS             3646..4494
                     /locus_tag="PW004_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HDL2726571.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4639..5580
                     /locus_tag="PW004_001596"
     CDS             4639..5580
                     /locus_tag="PW004_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HDL2726572.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5570..7741
                     /locus_tag="PW004_001597"
                     /pseudo
     CDS             5570..7741
                     /locus_tag="PW004_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7853..10024)
                     /gene="copB"
                     /locus_tag="PW004_001598"
     CDS             complement(7853..10024)
                     /gene="copB"
                     /locus_tag="PW004_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HDL2726573.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(10044..12230)
                     /locus_tag="PW004_001599"
     CDS             complement(10044..12230)
                     /locus_tag="PW004_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HDL2726574.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(12230..12439)
                     /gene="copZ"
                     /locus_tag="PW004_001600"
     CDS             complement(12230..12439)
                     /gene="copZ"
                     /locus_tag="PW004_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HDL2726575.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12452..12892)
                     /locus_tag="PW004_001601"
     CDS             complement(12452..12892)
                     /locus_tag="PW004_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HDL2726576.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12966..13505)
                     /locus_tag="PW004_001602"
     CDS             complement(12966..13505)
                     /locus_tag="PW004_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL2726577.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         3834 a         2843 c         2415 g         4523 t
ORIGIN      
        1 tacatcctaa aatataagct atttgtttac ttctgtagaa tatatacttt gttgttagat
       61 aagttgcaca tgtttataga atctaaataa taaattttta tgtaccgccc ctcaaagtga
      121 actgaacccc aaaagttgaa ctatttaatg gactgtttcc gatattctac tggagatagt
      181 ccattaaata gttcaacttt tggggttcag ttcaaagagg gggggatttt ttatcgttgt
      241 ggaatattta aataccaacg cttgtcatga aaatcttgtg ctccaccctt agtatttcct
      301 tctggatcgt ttgtcgcacg catcatgacg tatactttct tattagggaa atcacgcata
      361 ttgaaagata catgatagcc aacatttcct aaagtattat aagcttgatt tacgtctggt
      421 ctataaatcc catcagctct tactctagct agttcttttc cagtattgta gtccataatg
      481 aaaatatact cgtatttata attagcaatg tgccatccag ctacatgcaa gtttgcgttt
      541 tcgatttctc cgaactgatc aatgtgggcg taatttgttc catctgtcag cgtagaattt
      601 gcagcacctg ctcgagttgg atcaattact ggtttatcct ccgaagtagt tggattatca
      661 tccgtaaaac catgagccaa atcataggct agtttttctt tacttacacc catttcagaa
      721 agataaccgt aaggatctgt atgatcgccc caaatatttt gtgttaccca taaatgcgat
      781 ttgattcctg gttggttata aggagtgtct aatgttaatg gaataccata tttcattgct
      841 gaatctcttg ccaattcaac gtatacttta tagttctttt caaaagtttc tttatcatgt
      901 gtatgttgta actcgatttg cacaggactt ttggctcacg aggttgtcct actctacatc
      961 ttgctgatag atatgctgaa aggaaaacac caacattatg ttttgctttt tcagctagaa
     1021 cgagacaagc aggaatacct ttagaagata tcaaaggttt tttaggtcat aaagatgttt
     1081 ccactacaca agtctatgcc catatttctc ccgaagtgaa aaaaagatca atgaatcaac
     1141 tagaaaacta tatagaagag caaataaaaa agcactcaaa ttgagtgctt ttttaagctc
     1201 actatcacta aaactatcac taattttatt tataagcgaa catgtttcta tcactgattt
     1261 tttaaatcta gcgaaaaacc cttatcttaa aggcgttcgt tcaattcttt ggcaaggtct
     1321 tcaaagcctg gcttgcctag aagtgcgaac atggttttct tatagctcat gtagaaaaat
     1381 tttcttgatg atgattttat ccatcaattg agattgactg ttttgctctc ctatgatttc
     1441 agctatgatt gtccgatttt gcggttttct tcttaactgg tagttgattt tcccagtttc
     1501 catcaactcg ctaagcatat ccacgactcc gccaaatccg gtctttttcg taataaaacc
     1561 tagtgctacc attcgttcat ctagattttt tgctgtaatc gtcacaccag taatgagcga
     1621 gtgctgtaat gtttgggcca gctcttccat cccttcagca ttggatacga cggaagctgt
     1681 tttgttaata ggttcttcgt ttatttcttt tggttctttc ctctccactt tcactgtttg
     1741 acgaaggaca tctagttctt gcaagagcgt ttcgtattgt tcttgggtca gcatcacgcc
     1801 agctactttt tcacgattaa atatatatac gccagttccc gcgtctcgcg cttgagcaaa
     1861 tacatccatc ggcgatcgtt tcacttctgt gatcgaagat gtgggaactt ctaatttctt
     1921 taattccaat ggatttcctc ctttactaat cgctttttat tatagcacag actgacttgt
     1981 tttgatcttt tttccaaagg ttttatcggt ttttgcctaa ccagtaattg atatcggtaa
     2041 gaagtctttc gataccaaat gaaggatggt acagatacgt tttttgatct tcgttatggt
     2101 ctagtggaaa aaaatgatcg atgatcacta gatctgcata ccgaggcggc ttatttcgag
     2161 tacatacatg tatagagatc cgttttcgat gtcttagcgt tttgttcagt accccagcaa
     2221 gtcttcttgc ttcttggtgt tcatagcgaa aaaaaaagac gattttcact tcaggagggt
     2281 ggacatctaa ataccctcgt tcctggagtg ttttcagtaa aagcatcccc cactctactg
     2341 cggggtgtgc aagtgcttgt tggatcgcta gccgaaattg atatagttta tctttggaat
     2401 tgggaaattt ttctaatgtt tcctgataat aacgaacttc ttctatcagc cggtcatgga
     2461 taccaattac gtaacgaagt cattcttggt aaagtacttt tgtcagcttt gatttgcttt
     2521 cgctctcaaa ttcgctattc agccaaaaca gcgaatcaag cttttcagtc acaatcgtca
     2581 atttccgata gtcttgtgtc tcttctatcc gtttccaccg cttttcagta atctggttac
     2641 agctcaagcg caaaattgct agataatttt tttcttttat tggcaaaatc tgctcctctc
     2701 taaatagcaa ttcaggaaac aattcaaaga atagtgttcg actgtctcct aaaatctcga
     2761 tttctgccgg atcctttctt tgcttcaata attggatgaa catctcagaa aaggactgct
     2821 ctccatcatc tgcaaaaaaa tgcggaaaat ctttcaaaaa gcaaaacagc agatatctta
     2881 cttttctttc tgaaccaaag atctgtgctt ttttataaga aaaagaaagg tggtactcgc
     2941 caaaccactt tctcaatcgc tcttgcgtag aacgatcgta cttctgctcg taaggttcat
     3001 tcaatagcag atttttcagt aaacaaaacg ggggtgattg gatcagaaaa acaaacaaga
     3061 gataaggttc gctatatcct tgatcataaa ctaactgaac ggttttctta tgaagaatca
     3121 gctgaatggg acagtcgctt tccgccaaac gtttcagatg ccgatccact gttttaacat
     3181 cgattttcag ttcttcagct aaatcagctt ttcgccagat caaatctttc attagattga
     3241 ttagtgaaaa taaattctgt gcattttttg ataatagttt ttgccatctc atcgatcatt
     3301 tctcacctga tttctaaaca tattgtttga tcaaaatggg ttcactccta aaaagataaa
     3361 aaactccctt tctatttatt taaatacgca aatcttcctc attatttttt attttactca
     3421 atttaatcgt attctcattt caaaacgaat gtactttaaa aaaacataat aagatcactt
     3481 tgcttgtagg atgtattccc ttccattaca atgaaataaa tagcgatttt atgagaaggt
     3541 gatgtgaaaa tggaagaaaa aatagaagaa tccaaacaaa aaatcgatca gttgatataa
     3601 gaattagaaa aagcggtcat cggcaaacgt tcaactatcc aattgatgat cgtggcccta
     3661 ttagcaggtg gtcatgtctt attcgaagat attcccggtg taggtaaaac gttactggtc
     3721 aaagcactag caaaagctgt acagggaacc ttcagcagag tccaatgtac tcctgatctg
     3781 ctgcctagtg atattcttgg tttttccgta tacaattcac aatcaaaaga gtttgaattt
     3841 cgtccgggac cgatctttac cacgatcttg ttagcagatg agatcaatcg tacgactcct
     3901 cgtactcagt cagcactttt agaagcaatg gccgagaatc atgcaacgat cgacaacaat
     3961 acataccctc tagacaatca tttttttgtc ttagctacgc aaaatccaat cgagtacgaa
     4021 ggaacatatc ctttgccgga agcacagctc gatcgttttt tattccgact acaaatcggt
     4081 tatccttctt ttgatgacga acttttgcta cttttggata aattcgaaaa agtgttggat
     4141 aacttaaatg aagtattgaa cagttatgaa atagaagaaa taaaaacaaa tgtggcgaat
     4201 gtctttgtca gtcccgaagt ggcatcttat gccttgcagt tagtgtcagc aacaagaagt
     4261 catgctgcca tccagcttgg catcagtcca cgaggaagcc tttcctttat tcaggcagca
     4321 aaagcttatg ctttaatcca tgggagaaat tacgtcacgc ccaaagattt gcaggattta
     4381 gttccttacg tgttttccca tcgcctgatt ttttacgatc gttcattgaa ggaagacgaa
     4441 aagaaacaaa tcattcatac tcttgttgca caaattccta ttcctgtgag gtagattgag
     4501 gtagacaatg gaaaaaatca aagattattt cattaatttt gtgttatttc tggtatattt
     4561 catcattgtg ctttttgctc ttgtattcaa caattcttta ggctggttct tgttggcctt
     4621 ttttactctt tatgtatcat ggagcttgat ttcattaagc agctttctaa agaaactttc
     4681 ggttacttgt acattgcagc cactcgttta tcgcaaaaaa agtatgcagg ttattttttc
     4741 catagcaaaa aagagaactt tttctttacc tctccctcgt ttacaaatcg gcttcccagc
     4801 aatctttgca gaaaaaaaca agagattttg attcttacaa ataaaccgca agaagtccaa
     4861 ttgctttggc agccaaaaga aagaggatgc catgaaacat taactgtcat ttttacagcc
     4921 tatgatagtc taggactttt cagaaaaagt gcaaaaagag agatctcttt tcctgtgact
     4981 gttctgcctg ctttttgtaa gattcaagca gaaaaattac agagagttct ggaaaaaaaa
     5041 cagcagctga acgcgtacca aagaggtctt gattttcggg aacatcgcgc ttatcgtcca
     5101 ggtgattctt tcaaccgaat cgattggaaa ttgtctgctc atactcaaga gtggttatac
     5161 cgtgaatacg attatcaaga agaagaatgt tctcctcttt tttgcttttg gggttcgccc
     5221 agtccaaaat ttgaacctct gctagacttg tatttttctt tatggcattt gagaaaagaa
     5281 aagcagccgg aacttctgat attaggcaac cgcgcctttc atggcaaaga ccccggtcat
     5341 acctattttg cttctttgga agctggcgat gagaaagctt ttttcgcaca cttgaaaaaa
     5401 tatgcaaaaa aacaaatcgt cctctttgtt cctgagaatt ctcctgaagt ctcagaagct
     5461 attgaaacat tgtcaaaaga tcgaacggtt tatcttgttt atttcgtaga aaaacaactc
     5521 atggtccaag aaaaagacaa gatatgttca ttgccaggag gtgaatggat tgacgattaa
     5581 acgggaaaaa tggctgcgtg gcgtttactc ctttgtgata ctaagtgtag gtgctccgat
     5641 atttgtagta gctaatcaat tgcaaaacag ctaccttctt atcctaacta ttggtgtcct
     5701 atgccttatt gtctgctgta ttcctaaaaa atgggtacgt ttgctgcttt ctttgccagt
     5761 aatgataggt tgtttgtttt tatattttcc ttcgaagaca ttttccatat tatggatctt
     5821 acagtttttt tcgtcagtca cagaggaatt cttccatcta tcccaaggta aactttatta
     5881 tttgccagaa aagacagctt tttttctcat aatgttgctg atctatttat ttattacatt
     5941 gaccgtggat tacgactatt ggtacgcacc ctttctttct ttgatggctt acttcatgat
     6001 gcttaccgtt tttcggaaaa aagatttact gtacgaaatg attgcggtag taactgtttt
     6061 gttcgtgtat ctggcagcaa ggcaattttt ctccatccgg ttgttgtcgg gaaacactcg
     6121 attccagtca ttgactacag ttctgcttat ttgtttctta acttttagcg ctatcttgcc
     6181 tttatatctt ccgaaagtac accaatctct cgaggaaacg agtgaaccga tccgagaata
     6241 tcttaatgat gaagggcttt atgacacact ccatgagtat caattaaccg gaagtgcaag
     6301 aacaggcttc agtgagaatg atgaacagct tggtggacct ttatatgata atggcgaagt
     6361 tgtttttaca gcgaaccaaa aaggaaatgg ctattggaag atcgaaaata aaaatcatta
     6421 caccggttct ggatggatag aagatcagga cgcacccatc caaccaatcc aagaattccc
     6481 tgttgacatc acgagctacc aaaccgttat agaagacaaa gaaaacatta cattgaccct
     6541 aaaaaaacaa gttttcttac cttatcctta tggaaatact accttgactg atttttcatt
     6601 agctagtgga caaacgcttt actatttgcc tgaaagccag cgattcattc ctgtcccgaa
     6661 tgaatcgaca cgttctgcaa ctttacaaac agaaaggtta gtttttacga gagaagaatt
     6721 aaaaagcagt cagttaaaag ctgatgattt agaaaatcag cagacaaatc ttcaattgcc
     6781 aaaaagtttg cctaaacgta ttgaggactt ggcggtcgaa ttgaccaaaa atgaagcaac
     6841 tttaattcgt aaagtcgaag ctgtctaaca gtatttaaaa acaagcggaa atttccgcta
     6901 ttccaaaacg gataccgggc atactccaaa agaacacgat tatgtggatt atttcctctt
     6961 tgattcaaaa gtcggctatt gtgacaactt ttcaacttct atggtcgtcc ttcttcgaac
     7021 acttggtatc ccagctcgct ggacaaaagg ttttacaaat gggctcagaa caggcacaga
     7081 taatcaaggg aatgaggaat acagcattct caatagtcat gctcattctt ggccagaggt
     7141 atactttcct ggatttggct ggcttccatt tgaaccaacc ccttcatttg ccaatccaat
     7201 gggttcacaa ccagttgctg aaacaaccga aaccgtccag cctgctatcg aacaaccaaa
     7261 tgaaagcgaa aatatggcag aaactatttc cagtgagaga tctgaggtca ttccatcaga
     7321 agaaacaaca ctcacaccag cttataaaat cgtcttattc agccttggag tgtgtttatc
     7381 cgtcttgttg ttctttctat tgttattcag aaaatctctg cgctggacaa gttatcttat
     7441 ctatcttcat ttttttgctt ctactaatac gattgcatat caaaaacttc ttaacccttt
     7501 ggaaacattg gtaccgcgca aacccgcaga atctttgcag gaatatagcc tgcgaataga
     7561 acaagaattc aagctagatc tgcgtttcat tcgattaact actcaatatg aagcagaaat
     7621 atatggacga caagcagatc cggtaaaatt cgataaaaaa acctatttag aaataacgca
     7681 tataataaga aaaatggtat acaaaaaaat tctgacaaaa gggagccgct tcaaaaaata
     7741 agataaaatt tctggagaat ctacttctgg aatgccgaga accagctgat tgagtatcga
     7801 ttactcgaaa aaaaggttgt gacacgtttt ttgtcacaac cttttttcat tattatctga
     7861 ttttcaatgt caaagcattt agagctacaa ctaccgtact taacgacatc aagacagcac
     7921 caactgctgg acttaatatg ataccaattg gcgcaagaat tccggctgct agcggaattg
     7981 caagaatatt gtaacctgcc ccccaccaga ggttctggat cattttcttt cgcgtttgct
     8041 tggctagatc aagaaaatgc aaaatatctt ttggatcgct gtttgtcaat acgacatccg
     8101 cagaatcgat tgcgatatct gtccctgcac cgattgccat cccgattgaa gcacgggcta
     8161 aacttggcgc atcattgatc ccatcaccca ccatgattac ttttttccct tggtcaaggt
     8221 atttctggac aacagcttct ttatcatctg gtaataaacc gccataatat tcttcgatcc
     8281 ctaaataatc tgctactgct tgggcagcgt ttttattgtc accagtaagc atgacaggag
     8341 tgatttttct ttctttcaat gttcggataa attcttttgc ttctgttttt actacgtcac
     8401 ctaaagcaac gattgcagct agatgatttt ctaaaatcag gaagctgatg gtatttcctt
     8461 gttcttggta aggtttcaat aatgcttgtt ctacgtttaa tccgagacgc tcaacttctt
     8521 tttcattaac tattttcact tgttgttttt tgactgttgc ctctagcccg actcctgaaa
     8581 gattttttag attttcagct tgataaggct tgatttcatg ttctttcaaa taattcataa
     8641 tgccaacagc caatggatga ttggcattcg cttccaaggc tcctagatat tgaaggattt
     8701 catttttatt gaacgcatca tcaagtactt cgacacccgt tacagtaaat tttccttctg
     8761 ttagtgttcc tgttttgtcc agcatgatca catctagatc attggcttgc tccagtgcat
     8821 tgcggttttt caaaagtaat ccgtttttag ctgctattga tgtggaacga gcaacaacta
     8881 atgggatagc taaacctaga gcatgaggac aggcgatgat aaagactgtt accatacgtt
     8941 ctaatgcatt aggaagatct gtcaagaaca accaggcgat gaatgccagg atacccacaa
     9001 ttaatgcgac ataaaacagc cattttgcca ctttgtcaga taatgactct agcttggatt
     9061 tttctccttg tgcttttcgc accatttcca taaccttcgc tagataacca ttttcaccag
     9121 ttccagttac ttcgatttcg atcgttccat ccccattgat agaaccgcca atcactttgt
     9181 cattggcttg ttttttgatt cctcttgatt cgccagtcac agctgattcg tcaacaattg
     9241 tttcaccttt caagattaca ccatctgttg gcattttatc accagaccgg acgatcaaac
     9301 gatcaccttc gtttacttct ttcaatgaaa ctgtctcttc ttttccatct ttcgtcaagc
     9361 gtttcactga ttctggcagt aattctgcta gtttttgtaa agcattgctg gcattcgaca
     9421 cagcattcat ttcaatccag tgacctagta gcataataac gatcaaagta gccaattccc
     9481 agaagaaatc catgacgtgt gtatgtggat tgagcagatt tgcgataaac gaatagacac
     9541 tataaatata tgcaactgta atccccatcg cgatcagtgt catcatagca ggactttttt
     9601 gtttcaattc catcttagct ccacttaaaa atggctgacc gccatagata aatagtatag
     9661 ttgctaagat caatacgagc caatcagaac caggaaacgt tacttgaaat ggaaattcca
     9721 ttcccatcat tggtgaaaaa agaatgattg ggatagctag gataagcgat agccaaaatt
     9781 tttgtttgaa attccccatg tgcatggaat gatccatgcc gccgtgttcg tgatccatgt
     9841 gcatcgaatg gtccatttgt ttgctgtttg ctatatctcc atggttatgt cccatgtgca
     9901 tggaatgatc catctgacta tggtcatgtt tcatctccat ctgtttttca tgagattctg
     9961 tactttcttt gtttactact tcttttttat gctcatgttt tttctccata tcgttcccat
    10021 tcatttttac tccatccctt ctcttacttt ttcatgtgtc gatttaagca cagggagttc
    10081 aacaatacac tgacggaact aaacgccatc gctcctcctg caatgattgg attcaaaaag
    10141 cctagtgcgg caaaaggaat accaatcgta ttatagacaa aagcccagaa aagattttgt
    10201 ttgatttttc tcaatgtcaa cctagacaac tcgattgttt gtgcaatgga tgccaaactg
    10261 ctattcatca gcgtcacatc ggctgtttcc atcgcaatgt ccgttccact tcccattgcc
    10321 ataccgacat cagctaatgc taaggccggt gcatcattga ttccatcgcc tgccataccg
    10381 acttttttgc catctttctg aagtttttct acgtaggctg ctttatcctc tggaagtact
    10441 tcagcaaaga tatgctttgg atcaatacca acttgtttac caatcgtttc ggctgccagt
    10501 ttgttgtctc ctgtcagcat aaaaacatct aatcctttat tctggagctg cttgattgct
    10561 tgtttgactt ctagtttgat ttgatcagcc acggcaatca acccgatcac ttgcttttca
    10621 tcagctaaaa acatgactgt ttttccctgc tgttctaatt ctaacgcgtg ttcttggtat
    10681 tcatcaaatg ataggttcaa ttcaattaat cgtttacgtg tacccgcaaa atagcgtact
    10741 ccgttaatcg taccgctgat tccagcacct gggtgggctg taaaatcaat catatcgtat
    10801 gttgctgctt cttgcagttt tccgtattcc acgattgctt tacctagtgg atgttcagaa
    10861 gcttgttcta aagtataaaa aatagatagg acttctttag aaccgactag atctgtaact
    10921 ttgggctttc cttctgtaat cgttccagtt ttatccaaaa caatgctgtc taagtgagca
    10981 gctgcttcta atgcttctcc gcctttaatt aaaattccat ttcgtgcacc aacacccgtt
    11041 cctaccataa tagctgttgg agtcgctaaa cctaatgcac atggacacgc aatgactaat
    11101 acagagacac tatggatcaa tgcgagttgc caatcaccag tgatcaaacc tgtagctatg
    11161 agcgtgataa acgcgatgac taatacgatc ggaacaaaaa tccctgagat ccgatctgcg
    11221 attttttgga ttggggcttt gctcccttga gcatcttcca ccatttgaat gatttgcgct
    11281 aatactgtct gcttgccgac ttgtgtaact tccatgtgaa gtagaccatt cgtattgatt
    11341 gtcccgccaa agagcgtgtc atccggattc ttttctacag gaagattttc accagtaagc
    11401 atactttcat ctatcgcact gcttcctgaa ataattcgac catctgctgg aatctgctca
    11461 ccaggacgga tgactagttg atcaccaacg actacttctt caatcgctaa cgtttcttct
    11521 ttcccgtttc ttatcacttg ggcggttttt gtttgaagag acatcaattg tttgattgca
    11581 ttacctgttt tcgtttttgc tgtatgttcc aaatatttgc cgagcaaaat cagcgtgatg
    11641 atcatactgc tgctttcaaa ataaagatct tgggggtgtc cacggaaaaa tccgttgtaa
    11701 atgctcaatg caaaggcagc cgatgttcca attgctacca atacatccat atttggtgct
    11761 ttcgtcttga ttgcatggta cgcaccttta taaaaacgag caccgacata aaattggaca
    11821 ggagcagaca gcactaactg gacgattgga aaatgaaaaa agtgaacaat cgccgcatgg
    11881 cttcctaaca tcatagcaat cattgacaac atcaacggca aagtcaaaac cgtactcaat
    11941 ataagatcga atagcatttt tttcaaatac gcttgctttt cttctgcaat tttttgtttg
    12001 tgggcttcat cgtacaaaat tgcaccatag ccgatgtttt ctacgctttg gattaatttt
    12061 tcagtggtcg tcccttcata tttgacactt gctttttcag tggccaagtt gactgtggca
    12121 ttgaccacac caggttgtcc gttcagctct ttttcgatcc tagcagaaca atttgcacaa
    12181 gtcatacctg taatgacgaa cgtatccatt ttggtttctg ctgttgccat caaatcacct
    12241 ctgctttata acccaatcca ttgattgctt gacaaatctt ttcggattga acttttgttt
    12301 catcaaactt gaccgtaccg tttgcttttt tcaaatttac tttcactttt tgcacgccgt
    12361 caagtgcgct tactgtttct tctactcttg ctgcacaatg attgcagctc ataccttcga
    12421 ttgaaaattg ttgtttcatt ttttcttcct cctattgatg ttgtttacat tcacattgac
    12481 ctgggataca gttacattcg atcgtatcta ctggttgctt ttgttccagt tgtttcaaaa
    12541 tctcattgat atcttcttga gtcaaagtgg cttcttctac tagatccgca atcgtctctc
    12601 caatcctttt tgcacagata tgggaaaaaa gattttccgt cgcactgcga actgtttcca
    12661 tctccgagac agctggataa taaatgaatt tttttccttc ttgttctgtc cagatcactt
    12721 ctttttttac caaacgaccc agcaaagttt tgatcgttgc tggtttccaa tccatcgaat
    12781 cagctaagat ctgcgtgatt tcttgggctg tcgtctgtcc aagtgtccaa ataacacgca
    12841 tgatctccca ctctgaatca ctgatcttta ctggcgtttt gactttttcc atttatttca
    12901 cacctttcgt ttacaaatgt aatcattatc ttatgaatac agattacact tgtaaacaaa
    12961 aactgtcaat tatttttgct ctctattttc aatttagtta aaaattcttg tttctctcgt
    13021 cttgttttaa ggatgatcag tttatttctt gcattagcta cttggatcag ctgctgtatt
    13081 ttcggttcat tgttttcttc aaaatgccag ataaaagata aaaattctaa gtattccctg
    13141 tttagtttct ccgaaaatga ttcaggcata tctggccgag attttttgtt tatctttctt
    13201 tttagagaac gccaaactac tcggtagatg gcgagataac gcggcacttt aagccaaatg
    13261 atcttatctg cttctttaat cctttcctct aatgtagaag tataatttcc ttcaacgatc
    13321 catgacggat tagataccat aaacaattgc tgttcttgca aaaaccactt ttttgcctga
    13381 atactgtagt ctgttgtatg ccataaaagg tctagcggca gtagcggaaa gtcggtgatt
    13441 tctttgatct tccttgcgag ggtcgatttt cccgacccac tttgtccaat cacgatgatc
    13501 ttcattcttc tccctcactt ctctttggaa tttggttcga tcataccact tataacgccg
    13561 caaaaaaaca agggactgtg acaaaagtcg tgtcaaagtc ccttgtttcg aataa
//
LOCUS       DANKKG010000063        13602 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000063 DANKKG010000000
VERSION     DANKKG010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.063"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            474..716
                     /locus_tag="PW004_001603"
     CDS             474..716
                     /locus_tag="PW004_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HDL2726578.1"
                     /translation="MENELLENLGRLHTTELGVIRIKKNLSLNVENVIEWCKEKISLS
                     NAEITRKGKNWYITIDNCIITVNAYSYTVITAHKVK"
     gene            713..1183
                     /locus_tag="PW004_001604"
     CDS             713..1183
                     /locus_tag="PW004_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HDL2726579.1"
                     /translation="MINFSIKELNAFAVIGQEVELTNYQKKNIQISTQFWRKFNSSLK
                     KAYLSQSGNWVKYAFMERRNGKLYYFCSIPQRTITPEGFLYKEIPSYKYLVVEHIGAM
                     DKIYETYGKIYEEIIPSTSYIPIKDIILHFEKYDYRFHWNSDNSVIEIWIPIQD"
     gene            1311..2525
                     /locus_tag="PW004_001605"
     CDS             1311..2525
                     /locus_tag="PW004_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDL2726580.1"
                     /translation="MNDKNFIEELRQKREEYGVTQTRLAVACGISREYYNRIEKGKQP
                     LNDELREIIEKQIERFNPQEPLFLLIDYFRVRFPTTDALAIIRDVLQLKSNYMLYEDY
                     GKYGYESKYVLGDINIMCSMQEHLGVLLELKGKGCRQMECYLLAQERSWYDFMLDCMT
                     AGGVMKRLDLAINDKAGILDIPKLKEKYKAGECMSYFRMQKDYSGTEKCGSDLPKNTG
                     ETLYLGSTSSELYMCAYQKNYEQYVKNGTEIEDTEIKNRFEIRMKNERAYYAVVDLLT
                     YRDAERTAFSIINHYVRFVDREDDKPKSQWITNDDWAWFVGENREPIRLTTKPEPYTL
                     QKALHWLQRQVAPTIKMVQALDRENRTTILKDMIEQAELKDKHKHLLQLEKSTIEERI
                     DTAVPQENDGIF"
     gene            3514..3723
                     /locus_tag="PW004_001606"
     CDS             3514..3723
                     /locus_tag="PW004_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
                     /protein_id="HDL2726581.1"
                     /translation="MNCLERFYRKGVCVIDNAVKTIQKKNGKCGIGNRGKTKIVVKEH
                     FSEKGKTMEELLTDVMLEKAKQTIA"
     gene            3835..5448
                     /locus_tag="PW004_001607"
     CDS             3835..5448
                     /locus_tag="PW004_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2726582.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFDRPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAHYGA
                     YAPLGYKKHPDIKGKLLVDDETKWIIEKIFSLAYQGYGSAKITKQLRAEKVPTASWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAHVKAILKSEVYIGNSVHNMQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     GTNKTNKTPYSYYACSYYGQFGKGNCSMHYIRYDVLYQAVLERLQYWAKAVQQDEEKV
                     LNKIQKAGNAERIREKKKKASTLKKAENRQNEIDRLFAKMYEDRACEKITERNFVMLS
                     SKYQKEQIELEQQITSLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEATTNEDNERIQEIEIYYRFIGRVE"
     gene            5483..6694
                     /locus_tag="PW004_001608"
                     /pseudo
     CDS             5483..6694
                     /locus_tag="PW004_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            6717..7643
                     /gene="glsA"
                     /locus_tag="PW004_001609"
     CDS             6717..7643
                     /gene="glsA"
                     /locus_tag="PW004_001609"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HDL2726583.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            7851..9311
                     /locus_tag="PW004_001610"
     CDS             7851..9311
                     /locus_tag="PW004_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726584.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIREIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            9532..11295
                     /locus_tag="PW004_001611"
     CDS             9532..11295
                     /locus_tag="PW004_001611"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HDL2726585.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            11379..11837
                     /locus_tag="PW004_001612"
     CDS             11379..11837
                     /locus_tag="PW004_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HDL2726586.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            11946..12629
                     /locus_tag="PW004_001613"
     CDS             11946..12629
                     /locus_tag="PW004_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDL2726587.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            12776..13390
                     /locus_tag="PW004_001614"
     CDS             12776..13390
                     /locus_tag="PW004_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HDL2726588.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            13406..>13602
                     /locus_tag="PW004_001615"
     CDS             13406..>13602
                     /locus_tag="PW004_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726589.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNY"
BASE COUNT         4722 a         2159 c         2850 g         3871 t
ORIGIN      
        1 tttccgccat gcttgcaagg gggcggttcg cttcggcggt tcggcgagct tgcgagccgt
       61 tcaaagcccc cgttatctaa caaggggggc acaaaaaaca agaaacacta tacactaaca
      121 agcggaaaac gctgtattta taaggcaaaa ccccattttt acagtagtga tataaattgt
      181 acctatatca ctatgaaaat tgaatagtaa atatgagaaa gacagttgat ttcaaaaaat
      241 aagaaattga ttgtcgtttt cttttgcaga aatttaagtg caggcaatta tggtagttgt
      301 cgtaattgct gtcagctcag tgtttatcac tgatggtgtg aaagatttac ggttggagat
      361 gttcaataag attattggtg cttaattatt tttattgtag tgctgatatg agaatgttat
      421 aattggggta taaaacatac aaataaattt gctgaaaaat gaggtatcaa aatatggaaa
      481 atgaattact ggaaaactta ggaagacttc acacaactga acttggcgtt attagaatta
      541 aaaagaactt atctttgaat gtagaaaatg taattgaatg gtgcaaagaa aaaattagcc
      601 tttctaatgc ggaaatcaca agaaaaggaa aaaactggta tataacgatt gataattgta
      661 ttataacggt aaatgcgtac agttataccg ttatcactgc tcacaaggta aaatgataaa
      721 tttttctata aaggaattaa acgcatttgc agttattggg caggaagtag aactgactaa
      781 ttatcaaaaa aagaatattc agattagtac acagttttgg agaaagttta acagtagttt
      841 gaaaaaagcg tatctttcac aatcaggaaa ttgggtaaaa tatgctttta tggaaagaag
      901 aaatggaaaa ctttattatt tctgttctat tccccaaaga accattaccc cagagggctt
      961 tctgtataaa gaaataccgt cttataaata tttggttgtg gagcatattg gtgctatgga
     1021 taaaatatat gaaacttatg gcaagattta tgaagaaatt attcctagta catcgtatat
     1081 tcctataaaa gatattatcc tacactttga aaaatacgat tatcgttttc attggaatag
     1141 tgataattca gttattgaaa tttggatacc tattcaagat tagaagcaat tttatatcca
     1201 tacacacaaa gcagttagtc ttaggattga ctgctttttc tatacagatt tttaatgaca
     1261 ggcaattatc aattatggta gttgcctttt ttgattggag gaattgaaga atgaatgata
     1321 aaaactttat tgaagaacta agacaaaagc gtgaggaata cggagtaaca caaacaaggc
     1381 ttgctgttgc ctgtggtatc agccgtgaat attacaaccg cattgaaaaa ggaaaacagc
     1441 cattgaatga cgaattaaga gaaatcatag aaaaacagat tgagcgtttc aatccgcaag
     1501 aaccactatt cttattgatt gattactttc gtgtccgctt tcctactacg gacgcattgg
     1561 cgattatccg tgatgtatta caactgaaat ctaattacat gctttatgaa gattatggaa
     1621 aatacggata tgaaagcaaa tatgtacttg gcgacatcaa tatcatgtgt tccatgcagg
     1681 agcatttagg tgttttattg gaactgaaag gcaaaggctg tcggcaaatg gaatgttatc
     1741 tgctggcaca ggaacgctca tggtatgact tcatgctgga ttgtatgacg gctggtggtg
     1801 tgatgaaacg gcttgatttg gctatcaatg ataaagcagg aatattggat attccaaagt
     1861 taaaggaaaa atacaaggct ggcgaatgta tgtcctactt ccgtatgcag aaagattaca
     1921 gcggtacaga aaaatgcggt agcgatttac caaagaatac aggagaaacc ttatatctcg
     1981 gttcaacaag tagcgaatta tatatgtgtg cttatcagaa aaattatgag cagtatgtca
     2041 agaatggcac agaaattgaa gatacggaga ttaagaaccg ttttgaaata cgcatgaaga
     2101 acgaacgagc ctattatgcg gttgtagatt tactgaccta tcgggacgct gaacgcaccg
     2161 ctttttctat catcaatcat tatgtccgct ttgttgacag agaggacgac aagccaaaaa
     2221 gtcaatggat aacaaatgat gattgggcat ggtttgtagg ggaaaacaga gagccgatac
     2281 gcttaaccac aaagccagag ccttacactt tacaaaaagc gttgcattgg ctacaaagac
     2341 aagtcgcacc aaccataaaa atggtacagg cattagacag agaaaatcgt acaacgatac
     2401 tgaaagatat gattgagcag gcagaactaa aagacaagca taaacattta ttacaattag
     2461 agaaatcaac catagaagaa cgtatagata ccgctgttcc acaagagaat gacggtattt
     2521 tttaatgtaa ttgaaaaatt tcagatttag tcagcaaaga gctgttttca ttccctatgt
     2581 gaagtgaaag aagaaaaata aattttttat taaatctgca acaaaatggc tacttgcgta
     2641 cagatatggt gcgagaaaag gaaatctaaa ctttttttga aaataggtca ttaaatcggg
     2701 gcttgctgtc cctatatgaa gtgaaagaag aaaaaagttt tgaaaaaatg acggaaattc
     2761 gtgattagtg gacagtcttc tgtgaaaaga ggaaaaacat catcattttg tattcaaaca
     2821 ggcattagca gttgagtgtt gatgtgaaaa gatgaaaata ctttgttctc aacttaacaa
     2881 tttaaggttt ccgttcccta tatggtgcaa agaaaatatt tcattctatt gttggcagta
     2941 acggtatggt ttgggtagtt agggtgtgaa aaggaaaata ttcttttttc atcatcaact
     3001 tagcaaaatg acattttcta tccctatatg gtgcatgaaa agaagttaaa atttttccat
     3061 ttaaacttag caaatggacg tttgctgtac agatagtagt gaaaaaggag aaaatcaacc
     3121 gttgattgcc aaaatgaacc gttccatttg tactaataat ggaaaaagtt ttggattttg
     3181 gttacgtttc tcctcttttc caacgcgata tataggaaag acgatttttt tctttcaatc
     3241 gttctttgac aactgaataa agctattcaa tacgtttgac agacagctag ccgttgaaac
     3301 aaatacgcca cgacctttcc cctgcaagag cgagcgaaaa cctatttgtg ttcttgtaaa
     3361 agatttgtcc tcttttatcg ccatgacctg tactgtcaga taatgatact cccgtacagc
     3421 cacagcttga gcattaagag cgtcgcacgc aatgggtacg gctacataag aactatgcag
     3481 aggtggaact cctgtgggct atgacaaaag ccgttgaatt gcttagaaag attttacaga
     3541 aagggggtat gtgttattga taatgctgta aaaacaattc aaaagaaaaa tggcaagtgt
     3601 ggcattggca acagaggaaa gacaaagatt gtagtaaaag agcatttttc cgaaaagggc
     3661 aaaacaatgg aagaacttct aactgatgtc atgctggaaa aagcaaagca gacaatcgca
     3721 taaaatcggt gcagttgtaa gaaatagatt gaatgacaaa aagtacccgt gttatacttt
     3781 acttgcaagc aggtattgta aacacgggtt agttataaag gaggataact gacaatgaaa
     3841 caacagattt acaatactgc actttatctt aggttaagcc gagatgatga attacagggc
     3901 gaaagttcca gcattaccac acagagaagt atgttgcgtt tatatgcaaa agaacatcat
     3961 ttgaatgtca ttgatgaata tattgatgac ggctggtcgg gaacaaattt tgacagaccg
     4021 agttttcaaa gaatgattga ggatatagag gcaggaaaaa tcaactgtgt tgtaacgaaa
     4081 gacctttccc gtcttggcag aaattatatt atgacaggac aatatacaga attgtatttc
     4141 cctagtcata atgtccgcta catagcgatt gatgacggtg tagacagcga aaaaggcgaa
     4201 agtgagattg caccatttaa gaacatcatc aatgaatggg tggcaagaga tacaagccgt
     4261 aaagtcaaat cagcatttaa gacaaagttt gcggaggggg ctcattacgg tgcttatgct
     4321 ccgttaggat ataagaaaca ccctgacatc aaaggaaaac tgttggttga tgatgaaaca
     4381 aaatggatta ttgaaaaaat cttttcccta gcctatcaag gttacggaag tgcaaaaatc
     4441 acaaagcagt taagagcaga aaaagttccg acagcgtcat ggctaaattt tacaagatac
     4501 ggtacttttg cacatatctt tgagggaaaa cccgaaagta agcgttatga gtggacgatt
     4561 gctcatgtca aggcgatatt gaaaagtgag gtttatatcg gaaacagtgt tcacaatatg
     4621 cagtctacgg tatcgttcaa gagcaaaaag aaagtgcgta agcccgaaag tgaatggttt
     4681 cgagtagaaa acactcacga gccgattatt gacaaggaag tgttctatcg tgtgcaggag
     4741 cagataaaat caagacgcag acagacaaag gaaaaggcaa cgccgatttt tgcagggctt
     4801 gtcaagtgtg cggattgtgg ttggtctatg agatttggaa cgaataagac aaataaaacg
     4861 ccttacagtt attatgcttg cagttactac gggcagtttg gcaaaggtaa ttgttctatg
     4921 cactacattc gttatgatgt gctgtatcaa gccgtattgg aacgcttgca gtattgggct
     4981 aaggcagtac agcaggacga agaaaaggta ttgaacaaga tacagaaagc tggcaatgca
     5041 gagagaatac gggaaaagaa gaaaaaggca agtacattga agaaagccga gaacagacaa
     5101 aatgagattg accgtttatt tgcgaaaatg tatgaagata gagcctgtga gaagataaca
     5161 gagcgaaatt ttgtgatgtt gtccagcaag taccaaaaag aacagataga actggaacag
     5221 cagataacaa gcctaagaga agaactaagt aaaatggaac aggatatgat aggtgctgaa
     5281 aagtggattg agttaatcaa ggaatattcc gtaccaaagg aactgacagc accattatta
     5341 aatgccatga tagaaaaaat cctcatacat gaagcaacaa cgaatgagga taacgaaaga
     5401 atacaggaaa ttgagatata ttaccgattt attggaagag ttgagtaatc aacaagagta
     5461 atatttttaa ctaagggaaa ccggcggtgt ctatacttgg gtcagtcgca cattgggaaa
     5521 acgattcggt tttgccgcaa tctttttcca atggtttgag ataacagttg gctacttgac
     5581 catgctgtat ttcttaactg gtgcattatc ctatgcaacg ggtattggtg ctatacaaaa
     5641 caataaatat ctgaaattgg caatcctatt gattattttc tgggctattt tactctcaca
     5701 gttaaaagga accaaataca cttcattgat tgctcggatc ggttttgtag ctggtatctt
     5761 gttgccagca ttagtcttgt ttggcttagg gatccattat gtcgcttcag gcgctccact
     5821 acaaacgata ctgtctatga aaacattgat tcctgatttt tcgaaattgc ctacgctggt
     5881 tgtttttgtt tcctttattt tagcatatat gggcgtagaa acatctgcca gtcatgcaaa
     5941 tgaaatggaa aaccctaaaa aggattatcc attagcaatg tttgtattgg ttattctggc
     6001 aatcatttta gatacttttg gcggattgac cgtcgctact acagtacctc aacatggttt
     6061 gtctcttaat acaggggtga ttcaatcgtt agaattccta ttaagacaca ttgatccaaa
     6121 tcttgcatgg attgcgaaaa ttcttgcgat tcttgtttgt ttaggtgtca tcggagaaat
     6181 tgcttcttgg gtaacctctc cttcaaaagc acttcacgta gcagcagaag aaggattact
     6241 tcctgaatat tttgcaaaag aaaacacaca cggtgttcct gctcatttga tgatcgcaaa
     6301 tgggatcgtt gcaacaatct gggcagccgt ctttacatta agcggcgggg ggaataatct
     6361 ttcatttctt gcagcgatgt cattgactgt tgtaatctac ctgatgttgt actttatgtt
     6421 tttcgcgggc tatttgaagc tggtttttaa agatgacagt cttgaaagag cctatcaggt
     6481 tccaggcgga aaagttgtaa aagtaatcgt tgcttgttta ggtcttttga cttcactttt
     6541 tgctttttgt atctcatttg ttccacctgc tagtatcgca gctggacagc atgggattta
     6601 tgaaacgatt ctgattgttt cttatcttat cgtcttagtt attccattta cgatctacgc
     6661 atgccgtcat aaattccagc atgcagaaaa ataaaaaatg aaagctggtt attcaaatgg
     6721 aagtaaaaga attagaacaa ttgattggaa aaaatttcca ctattatcaa acaggacaag
     6781 tggcggatta cattccagca ttagctgaag tggatcccaa acaattaggt atggcgattt
     6841 atgatttgga tacaaaacaa ctgattgagg gaggagacgc cgatgtgcgc tttgccatcg
     6901 aaagcatgtc aaaagttccc gtattgttat tagcaatcaa agacaacgga atagaaaaag
     6961 tctttcaaac aatcaacacg gaaccaaccg gttttgcctt caattctatc atgaatatgg
     7021 aaatcaatca tcgcaaacat ccaatgaatc cttttgttaa tgcaggagcg attgcaacaa
     7081 cctcgttgat tgccggtaaa gatgcagatg aaaaatttga gcgaatcttg tcatttatga
     7141 aagagatctg tgatgatcct aatatcacat tgaatgaaga aatctatcat tctgaatcaa
     7201 gaacaggtga tatcaatcgc tctcttgctt actatatgaa aggtaatcaa atgatcgaag
     7261 gagacgtacc ggagatactc gatgcttatt tcagacaatg ctccctcaat gtcaccgcta
     7321 caggcatagc aaaattagct gctgtgctgg caaataaagg aattgctcct tggaatggca
     7381 aacaaatcat tactgaagaa agtgctacaa tcgtcaaatc aattatgaca actgctggtt
     7441 tgtatgatga atcaggagag ttttccgtcc atgttggtgt acctgcaaaa agcggtgtag
     7501 gcggcgggtt gatggcagct gtgccaaatc gttacggcat aggtgtcttc agcccggcac
     7561 tagatccatt tggtaacagc gcagctggta tccaattgtt gaaagatgta gtcaaagaac
     7621 tagacgctga catctttgaa taaccattct tgaaagaggc tggggtaaaa gtccaaacgg
     7681 ctcaaagcta cctacaatga ggctgaaatt gtgagcttgt ttgacttttg tcccagtttc
     7741 tttttttatt ggtttttgaa agggcttttc taaataaaaa ggaaacaaaa atacgattta
     7801 cagatatgat taaataaaat cataagaaaa actggaggcg tgtatcagga atgctggaaa
     7861 attacatcga gcgaaatata tttagaaaag tgtatctgtg tgaacaatta tttgaattcc
     7921 aagaaataga catcgaacaa acagctattt ctctaagggt gactacacca acgatccttc
     7981 acgatctaga aagtctagcc gagtgtctag aatattgtat aaaagaacaa gtaagagaga
     8041 agcataagta taaactcgta ttcaaacatg gtattgctct ttctgagctc acacagtttc
     8101 tgtacggtca atcttatttt ttaaagtttt taagttatta ttttaatggt atattcacga
     8161 gtacagaact ggctgatctt gaatttattt ctttgagtaa agtatataca atcaaaaaaa
     8221 tcgtattgga tttttttaaa gaaaatgatt atttgaaaaa taaagaaatc attatcccag
     8281 aatttgactc gcggaacatc ttgctagctt tagtacgtta tgtcaattgg gaaggctatg
     8341 aaaataaaaa tccgtatgta gaagaagctt gtaacgagtt gattgattat gtagagactc
     8401 atttttttaa aagaagatat tcggaagatg aaaagtcctt tatcatacga gaaatagaga
     8461 tcgcaatcgg aagaaaggca tttccccttg gactgaaaga agaagataag aaaagcgcag
     8521 aaacgaagcc gttatttcaa ttagtctgtc aaggattgga agagcaaaaa ggaattctag
     8581 acttacaaga agaagatgtc tattatattt tttctttgtt caacagtaga aattatacca
     8641 atgagaacat ggagctgtta agaaaagatt tcgaagttgt ttataaaaat tttgttctca
     8701 atgacctatc actgaatgaa ttgattttac agattattag tatcgctgaa atcgacgatg
     8761 cagaaaattt tattttcaag caatcctttt tacaatttgt ccgtacgttg tgggcagatg
     8821 gacaagtatt tttaccggaa aggatctatc ttctatcaga aaaagaacaa cttctgtacg
     8881 gaaaaattgt agaaatattg gaagagtgga aaaagaaaaa tcaaatccaa ctaagatgga
     8941 atgaaaattt cattcgaaaa ttcgtcaaaa gtctttcttt gatcaacagt gaacaagcgg
     9001 ggagacagga aatagaaatt tttgttgttt cagaaagttc agtcaaacaa tttttttatc
     9061 gaacccaatt tcatttgtat gcggaagaag gcatcgctga aataaatcct attttatttc
     9121 gttcattaga agaattgcct gatgaatgtc tatgtgcgaa aaaaagagtg gttttgtgcg
     9181 atgctgctag ctatcaaaat ggattagaaa ctgaaaagac aaagatctat ccaatctcat
     9241 taaaaggtgc aaacatccat ttgtatcata ttgctcagga acttcaccta ttaaaaaaac
     9301 atatttcttg atgagacctt aattaggaga ccttctacaa agctttttaa ttgtctgtag
     9361 aaggttttta tttgagtcct gattcgtgcg aagatctact tatataacaa gctttctctg
     9421 aaattttttt aaaatgctat tgacttagag tcaactctaa gtagtagaat agataaagtg
     9481 taacaaatct attttcatta aaagaagata gtacggagga gagaatgtat tatgagtaca
     9541 gtattacatg tattggatgc aatcaatagc cccgcagatg tcaaaaaatt atctgttgag
     9601 gaaatgaaac agttggcaga agagatgcgc caaaaaatct tgcaaaagga cagcgatgta
     9661 ggtgggcacg ttggtccgaa tctaggtgta gtagaattaa cgattgcgtt tcattatgta
     9721 tttgattcac caaaggataa aatcgtttgg gatgtttccc atcagtctta tggtcataag
     9781 cttttaactg gtcgaaaaaa tggttttgaa aaaggacatt accgtgaagt gagcggatat
     9841 tccaaccaac atgaaagtaa acatgactat tttacagtgg ggcacacatc tacatctatc
     9901 agcttagcaa caggtttagc taaagcgaga gatctgaaga aagaatcagg aaatgtggta
     9961 gcatttatcg gtgacggctc cctaagtggt ggcttagctt ttgaaggatt gaacaatggt
    10021 ggtgttttac aatccaatct aatcgtatta gtaaacgata atgagatgtc tattgatgag
    10081 aatcatggca gtttatataa aaacttagct gagcttcgtc aaaccaacgg taaaagcagc
    10141 aataacctat ttaaagcaat gggatttgat tatcgttacg tggataatgg aaacgatctg
    10201 gaaacggtga tccgtttatt tgaagaagta aaagatattg atcatccgat tgtacttcac
    10261 gtccatacgg aaaaaggtcg cggttatcag ccggcaatcg ataataaaat gaaatatcat
    10321 tggcatgtac catttgatct gtccaccggg gaggcaaagc aagtaacttc tggagaaaca
    10381 tttaatatga tcatcatgga ttatttggat aagaaaatgc aaacaagtac agctcctatt
    10441 gccgcagtca atgcagcgat accaggcgcc tttggactga aaacattcca agaaaaacat
    10501 ccagatcatt attttgacgt ggggatcgct gaacagcact ctatcacttt cgcttctgct
    10561 ttggcttctc agggagtacg tccagtcgtc ttccaatcaa gtactttctt gcaacgggca
    10621 tatgatcaat tatcacatga tttagcgatc aacgagaatc cagtgattat tttagtcggt
    10681 ggcggaacga tctcagctag tgaccgaaca caccaaggaa catttgacat tccttactta
    10741 tcaagtatcc cgaacatcaa atatcttgct ccagcaacaa aagaggatat gatctctatg
    10801 ctagattggg cgttcgaaca aacagcgcag cctgtagcaa tccgaattcc tacacatggt
    10861 gtagaacatg gtacagctat cgcaaatgat tatagtattc cagcctatca aacagtaaat
    10921 aatggaggaa aagtagcaat tttagcttta ggcggttttc ttgaattagg aaaagaaaca
    10981 gtaaaagctt ttagaaaaaa aggaatcgaa gcaacgttga tcaatccagg aacgatcaca
    11041 gaaaatgacg tagaaacatt agataaatta aaagaaacac acgaattagt catcacatta
    11101 gaagatggta gtttgtcagg aggatttggc gaaaatatca gtcgttatta tggtgattct
    11161 tcaatgaacg tattaaattt tggtgcagag aaagaattca cggatagcgt tcctgtaggg
    11221 cagctttatg aacgttatca tttaacacca aaacaaatcg ttgaagatag tctgcgtgtc
    11281 ttgaatattc aataattcat tatgctcacc atccagataa ttgtttatac tagtatctgg
    11341 atggtttttt cattataaaa agcaaagggg tggatgttat gtatcagcaa gtagaagaat
    11401 atgtcaaaaa gttccaagaa aaatatccta gttatcaatc agaaaaatta ggtttctttt
    11461 catttgggga tacagaaaaa atggcagacg agttgagcga actagttgta aaaaagataa
    11521 agactggtac atcaagtggt tacgatctgt ataaacaaga gaatgagcct ttaccaaaag
    11581 ttggtcaatt agacgtggtc ctgaatggaa aaaatgaacc agtttgtatc atcaaaaata
    11641 taaatgtgac catcttgcct tttagtgaag tcacgaaaga gcatgcttgg aaagaaggag
    11701 aaggggatcg gacattagct tattggcgga aagtccatgc tgagtttttt aaacatgctt
    11761 ataaagaagc gttgaacata gaatttacag aagagaagct ggtcgtatac gaagaatttg
    11821 aagtaatttt cttttagtaa gaaaggtctg ctgaaagaaa tagctcatta ttgtctcctg
    11881 aatagaaaag ggttacattt ttatatgaag ctgtattttt atataacatt actggaggag
    11941 atattgtgga tacactggaa caaatcaaag gcggagtcgt cgtttcttgt caagctttgg
    12001 aaaacgagcc gctacacagt tcttttatta tggggagaat ggctttggct gcaaaagaag
    12061 gcggagcagt tggtattcga gcaaatacga cagaagatat caatgaaatc aagcgaatcg
    12121 ttgatttacc ggtaataggg attatcaaaa gagattacga tgggtcagaa gtgtttatta
    12181 ctgcgacaga aaaagaagtg gaagaacttt tggagacgca agcagaaatc attgcactgg
    12241 atgcaacttc tcgtaaacgt ccgacagaaa aaacaactgc tgaacttgtc caaatgattc
    12301 acgaacacgg tcggttagct atggcagata tttcgacttt tgaggaagcg cttcaagcgc
    12361 aagaagatgg ttttgatctt ttgtctacga ctttagcagg ctatacagaa tattcacgaa
    12421 agacgcagac acctgatttt gatttattga atcaaatcat agaacaagtc gatatcccag
    12481 taattatgga aggacatact gactctcctg aacaagtaga gaaagcatat gaattaggtg
    12541 ctttttcagt cgtagttgga tctattatca cccgtccgca attgatcact aaaagatatg
    12601 tcgaagccgc aagtaaagca aataactaaa aaacacgcac ggcacagatt cttgacaaaa
    12661 aagaaaaatt gatagatagt ttaaagcaga ttctaagaag atgaaaaaag cattactttt
    12721 cattttgtct tcaagatctg ctttttaacg aattttatag aaagaaggcg aaaccatgtt
    12781 tgaacggagt ccttcagaat tgacagaacg tgaaaattac aagttgctga ttggttcagt
    12841 catccctaga ccagtagcag tagtaacgac taaatcagca aaagatatag tgaatatcgc
    12901 acccttcagt tacttcaata tcgtcagttc gaatcgaccc atcttatcct tggcgatcca
    12961 gagaaaacaa gatgcagtaa aagacacagc aaagaatatt cttgagacaa gagaagctgt
    13021 tatccatatc cttgatgagg acaacgtaga agaagtaaat aaaacagcag ccaatctgcc
    13081 taatgaggaa agcgaactgt acctgactga cttgacttta actgattcag taatcatttc
    13141 agtaccagca ttgacagaag caaaagttcg ttttgaaaca tccttatatg aacatgtcga
    13201 gatacggaat aaaaataaag taacagctga tttatttttg ctagaagtac agaaatatca
    13261 tttaaatgaa acagtctatg atgataagac aggaaggatc gatccaagaa aattgaatgc
    13321 tgtaagccgt ttggcaggaa atgattatgc agcaatagga gaaattttca cgattgcacg
    13381 tcccgaataa aaaggagcac tagttatgtt aaaaataagc tataaaccat cgacagattc
    13441 aaaagaaatg aaaaaagaat atgaaacagt gaatgatttt ctgcaagggc agtacttaga
    13501 agttccgcca ttgcaagacc attttgtcgt tactactgtt actttagatg gtaaggaaat
    13561 agaaatgcca gatcaaacga ttagcggatt gtttaattac tt
//
LOCUS       DANKKG010000064        13571 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000064 DANKKG010000000
VERSION     DANKKG010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.064"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(93..1040)
                     /locus_tag="PW004_001616"
     CDS             complement(93..1040)
                     /locus_tag="PW004_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HDL2726590.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            1230..1499
                     /gene="rpsN"
                     /locus_tag="PW004_001617"
     CDS             1230..1499
                     /gene="rpsN"
                     /locus_tag="PW004_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HDL2726591.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(1675..2037)
                     /locus_tag="PW004_001618"
     CDS             complement(1675..2037)
                     /locus_tag="PW004_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDL2726592.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(2048..3169)
                     /locus_tag="PW004_001619"
     CDS             complement(2048..3169)
                     /locus_tag="PW004_001619"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDL2726593.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(3185..3535)
                     /locus_tag="PW004_001620"
     CDS             complement(3185..3535)
                     /locus_tag="PW004_001620"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HDL2726594.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(3665..5176)
                     /locus_tag="PW004_001621"
     CDS             complement(3665..5176)
                     /locus_tag="PW004_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDL2726595.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(5498..6850)
                     /locus_tag="PW004_001622"
     CDS             complement(5498..6850)
                     /locus_tag="PW004_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HDL2726596.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYLEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(7003..8079)
                     /locus_tag="PW004_001623"
     CDS             complement(7003..8079)
                     /locus_tag="PW004_001623"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDL2726597.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            8275..10020
                     /locus_tag="PW004_001624"
     CDS             8275..10020
                     /locus_tag="PW004_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HDL2726598.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(10300..10737)
                     /locus_tag="PW004_001625"
     CDS             complement(10300..10737)
                     /locus_tag="PW004_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HDL2726599.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(10721..12094)
                     /locus_tag="PW004_001626"
     CDS             complement(10721..12094)
                     /locus_tag="PW004_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726600.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(12416..12904)
                     /locus_tag="PW004_001627"
     CDS             complement(12416..12904)
                     /locus_tag="PW004_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2726601.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(12926..13528)
                     /locus_tag="PW004_001628"
     CDS             complement(12926..13528)
                     /locus_tag="PW004_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDL2726602.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         3884 a         2944 c         2459 g         4284 t
ORIGIN      
        1 tattatacgt atacgcaaaa aagctaaaac attttttgtc tttgccccta caaaaatgtt
       61 tcagcttttt tcgtttttta ttcctgtatt tcttattttt cgaatgacag gatgattttc
      121 ccagcaggag gatgtccttc aactagctga tgtgcttgga tgacatgctt aagtgaagca
      181 gacagattca ttgaaataaa aacatgaaag gctccttttt ggtaggcttt gattatgcca
      241 gcaaaggctt ctgcatctag atattccttt cttggaacaa acgattcata aaagccttcc
      301 tttttttgcc gcaaatctag atctgggagg tcatttaatg caacatacct gccgcctggt
      361 ttcatgatct ggatgccggt ttctcctttg atacttcctt tcgtcgcatc gattacaagg
      421 tctgcctgat cagcaaattg cagacctgga tttgttttat cataagcagc aaatgctgaa
      481 gctcctaatt tttttacctt ttcctcattt ttactggatg cactggttaa gattcggatt
      541 cccttttcat ggagaagctg gatcagactt gacccgaccg cacctgatgc tcctagtatc
      601 attacagtat ctgtcggctg gatttccgtc aaatggttga ttagattata agccgtaatt
      661 cctggcgtga ccattccggc tgcttcttcc caactcattt tatctggaat ttttgctact
      721 tttgctgatg gcaacacgac ttcttctccg taagtcccgc cgactgcatg gacagctact
      781 cgatcaccta catgaacagt tgtgacatct gaaccaatct ctgtcacaat accagcgcca
      841 tcattacctg gtacataagg gaatttcaat gttcgtatct ctttcatttc tcctaaacgc
      901 aaggcaacat cataaggatt cacactgaat gcttttattt ctacacgaac atgtccagaa
      961 ccgacttctc tgggatgagc atcgatttct tcaaaaacat cttgtgcttc tccgtaaccg
     1021 ttaattgcaa atcttcgcat ggtcaccatc tccctttttc tttaagttta cgtttatgcc
     1081 tttattttga caaaaaaaat gtcttttcgt tttggcgcat gattcaaaac aaaatagttg
     1141 tcttttttcg cgtaacgtgt taaattattt atctaaaagc gtaaagatta cgatttacgc
     1201 taataaaaaa taattggagg aatttgttga tggctaaaaa atcaaagatt gccaaagcaa
     1261 aaaaacagca agaaacgatt gaacgctatg ctgctttacg ccaggaatta aaagcgcaaa
     1321 aagattacga aggcttgcgt aaacttcctt tagatgctcg tccagaacga ttgaaaaatc
     1381 gtgatttaat cgatggacgc cctcgcggat acatgcgtaa attcgggatg tcccgtataa
     1441 aattccgtga attagcacat aaaggtttga ttccaggagt aaaaaaagcc agctggtaat
     1501 ccaagtctgg ctgtaatgta aaggaggagg gcgcatggac cactccttgg atcatcaaaa
     1561 aagatacgtt cgcttcttct agcagaaatg ctggtactag aagcagacgt atcttttttt
     1621 acgcacagct ggtccttttt tatatgtaaa ggctgcatgg ttacttcgct atatttattt
     1681 agtaacagga gtcagtgctt cgatacccac actaattttc aatgctcggt caacttctgc
     1741 cataatgggt cgatccaaat ggcagacctt ttctttcaat cttgatttat cgatcgttct
     1801 gatctgctcc aacaggataa cggagtctct ttctattcct gttccagtag ctttgatccc
     1861 aatgtgagtc ggaagcttag gtttagccat ttttgccgtt atggccgcaa caatgatcgt
     1921 cgggctaaaa tggttaccta gattattttg aataaccaaa actgggcgtg ttccgccttg
     1981 ctccgatccc acgactggag aaagatcagc aaaataaata tctcctcttt ttaccatagg
     2041 atcctcctta ttcttgatat tctcttggta tccgctgtcc taatccgcaa gccacttcat
     2101 agtggatcgt acctaattga tctgctatat cctgcatagt gatttctttg tctgcatttt
     2161 tgccgataag cgtcactttt gttccgacag gaaattcttg aggcaatcgg atcatcagtt
     2221 gatccataca tacacgccca atcgtttcac agtagttccc ttctaccaat agagaaaaac
     2281 cctgcatctt tcgtggccag ccatctgcat aaccgatcgg tatcgttccg atccactctg
     2341 cttcaggagt gatatatgtt tctccatagc cgattccttc acctgctggg agttttttga
     2401 cttgaatcaa ttcagaaacc agctcaagtg ctggctgcaa tggataaact tccggtagtg
     2461 cgtgcccaga gggattaagt ccgtacattg ctactccata acgaatcata ttgccgattg
     2521 tttcatcatg ccataaagcc gttgcactat tgctgacatg tacatatcga ggaagttcgg
     2581 gaagtttttt cagcacttct ataaagcgtt ctttttgcag attccaatat gtatcatctg
     2641 cctgatctgc tgtagaaaaa tgggtaaata aaccttccca ttctaataca cgactttctt
     2701 tgatgaatgc agctgcctct tttacttctt caggtgtaca aaatccgata cgacccatcc
     2761 cagtgtctgc ttttagatga atcgaaagag gcgtctctag cttacttttc tctaaaacag
     2821 cagctgctgc ttgcaaccat tcccgtgtac ctgcagttac tgataaatca tgagcaacag
     2881 ctacagtcag tgcgtcgtaa ggaaccatat tcaagataag gatgggctga gtaaatccag
     2941 cttctctcaa ctcgactcct tcatctaaat ttgaaacaca aaagcccgaa gctccacctg
     3001 caactgctgc ctcagctgtt tcaatagctc catgaccgta tccgttcgct tttactactg
     3061 caaacaattc ttttccttgc ggaagtcttg caacttcatt tgctacattt tcagttatag
     3121 cttttttatg tatcactgct ttcgttgggc ggtgccaagc gacaaccatc gtttttcctt
     3181 ctttctagtc ttccaaaata atttgtgcaa aagctgtttc atttgtatga gtaatactga
     3241 cccatacatt gccttcatga ggtgaacgcg tcacaacagg ctgaccattt tcatttttta
     3301 aaatctcgac atcttgaaag gtaacttttc cgatcccagt tccccaagct ttagagaaag
     3361 cctctttgca agcgtaacgc ccgcctaaaa attctacttg gcgatgaaaa ggcaaggact
     3421 ggaatagttt catttcatca gaggtcaaga ttcgtgcaat gaattttggt ttttcttcga
     3481 tcagccgagt gatcctagga agttcgactg catcgatccc aattcctttt atcatatgct
     3541 tttctctttt caataaattt agaacactct cattctacca aattataacc attttgccac
     3601 aaataagtcc acaaataaca aaaatgaaag tttctcttaa aaaagagaaa ctttcatttt
     3661 atgtttaatc gttatttgaa cggattacaa atccgcgttt tttatcattg tgacgtttac
     3721 cgccatcttt ttggctgtta ttatttttgc tataaccgcc ttttgatttg tttccacgat
     3781 aattaccgcc gtcacgacga ttacggttgt ttccgccacc acgtttgcca tttttattgt
     3841 atcctttttt ctgcattggc aatgggcgtt ctggtgtgat cttcactggc actgcatcag
     3901 ctggatcttt tgctgtcgtt tttagcaaaa gtgcaactaa atcttgtgca gaatattctt
     3961 ctaataattt atctgctgtt tgcaggtatt tatctagacc attttctgca agttttgttt
     4021 cgatttgttc aactgcagca cctaattgac ctttgaatgc ttctttttcg gttggtggac
     4081 gtaaagtcgt catccgtttt ttcgtcaagt tttcaatcac atgcaagtag tccatttcat
     4141 ttggtgtaac gaaagtcaca gacataccgc cttttccggc acggcctgta cgtccgatac
     4201 ggtgaacgta gctttctgga tcttgaggga tatcatagtt gtacacatga gtgacacctg
     4261 aaatatctaa tccacgtgca gcaacgtctg tcgctacaag gatatctaaa tgaccgctct
     4321 taaatgaacg caatacgctc attcgttttt gttgagacaa atcgccatgg attccttctg
     4381 cacggtaacc gcgtgcttct aatccgcgtg ccaattcgtc tacacggcgt tttgtacgtc
     4441 cgaagacgat tgttaattca ggtgtttgaa catcaaacaa gcgagtcatg atatcgaatt
     4501 tttcgtattc ttttgcgcgg acatagtatt gatcgatcaa gtcagctgtc atttctttcg
     4561 ctttgatctt cacatgatgt ggatttttca tgaatttaac gccaatattc ttgatagctg
     4621 gcggcatcgt tgctgagaaa agtaatgttt gtctttgatc aggaacttgt gaaatgattt
     4681 tttcgatatc ttccaagaag cccatattca acatttcatc tgcttcatcc aaaactaatg
     4741 tttgaacggt tccaagtttc aatgtgtggc ggttaatgtg gtcaagcata cgtcctggtg
     4801 tacccacaac gatatgtgga cgatctttca agccacggat ctgacggcca atatctgctc
     4861 cgccgtaaac ggcttgtaca cgtacttttt tgtcgcgccc taaacgatat aattcttctt
     4921 gtgtttggat agctaattca cgtgttggtg cgataactaa accttgtaat tcgtgacgat
     4981 ctggatcgat tttttctaac attggtaatc caaatgcagc tgttttccct gtacctgtct
     5041 gtgcttgtcc aataacgtct tttccagcta atgctagtgg aatcgtttct gcttggatag
     5101 gcgttgcttc ttcaaaacca gcgcgttcga ctgatttcaa caattctggt gataattcta
     5161 gttctttaaa tttcaaatga atcctcctat atggttgtta actgaggatg tagctttttt
     5221 gctaccccgt cgatttgttc ggtcaaagtg tattgtttct ttttgacagt ggccacgcac
     5281 gtttttatcc attcttagcc cctgtggcac caccgaaaaa tcactttgcg aagtttaggc
     5341 attagaaaag ccaaacgggt cgcgttcagc tttatcatta ttatgacatc tggcttcaac
     5401 aagttgtttc tatggcaaaa ccagtccaat ttctcttcat ctttcggcaa taggttattc
     5461 taacatagtt tttcgttttc tgcaagttta cagattttta tttcatttgc tgaagttgtt
     5521 ctaccacttc aaataagccc atgctgttgc tgccttttag cataatacta tctgttgctt
     5581 caagattttg tttgatagca tcaatcaatt tattcttttc tttttccaaa tagaacacag
     5641 gtagttgggg gtatttttgg ttcaattctt cttttagtgc atgcatttgt tccccataaa
     5701 ggaaaatcat ggcgaatttc tcaggatcga tatgttcact catttgcgca tgcatattta
     5761 aagactcttt ccctaattcc agcatatctg ctaacacagc gattctgcgt ccttttgttg
     5821 gcagattttt aaaagtatct aataccagtc ccatggctgt tggatttgca ttgtagacat
     5881 cgctcaaaat agctgcacca tttcctgccg ttagccattc ggttctgttt tgagtgatag
     5941 aaacatgggc gagcccttgg cgtatctcag gtatggacag tccaaagaag cgcccgatcg
     6001 tatacgcaat caaagcattc gttacattat aacttccagg cagcgggatc gtaaatgttt
     6061 ctccttctac tacaaaatcg gtttgtgctt ttccttttgc tgtcacttcc gcatgaacat
     6121 cgccatttcc aataccgaat gttgtgactg tttgtactaa tgttgccgtt aaaggtgtca
     6181 gcaacggttc atctgcgggc acaacgagta atccgtctga tacaagtccg tcagtgattt
     6241 ccattttggc tttagcgatt ccttctcgcg acccaagatt ttctatgtgt gcttcaccga
     6301 tcattgtgat tgctgcagct tttggttgag ccataagtga aagttccgtg atttcattgg
     6361 cgtgatccat gcccatttcc aaaacgatct tttctgtatc ttggggcatg tgcaaaatcg
     6421 tgtaaggaag accaatatta ttgttgtaat ttccttgcgt cttatatgtt ttgtattttt
     6481 cagacaagac agacgcggtt agatctttcg tggtggtttt accattgctt cctgtaattg
     6541 caacgacttc tgcgcctact ttttccaagt aataggttgc caattgctgc atggctttca
     6601 aaggatctgc tacaggaata actggaaaat tttttggagc ttgttcaaca ggcaagctcc
     6661 aaaatgaagc agatgctccg tttgtgatcg cttgctggat aaattcatgt ccatctcgcg
     6721 taccagcaag aggaacgaat aaacttcctt cggttatctt ccggctgtca aattcgacat
     6781 tggtaatctt tgtcagtgca gagcattcgc tgcccacggc ttgtgcaatt tcttgtattg
     6841 ttagattcat tgagatctct cctaaaaaag gttgtgaaaa aagcgtttgg cctaaacggc
     6901 gggttcgcat gactctcact tccaagtaaa tttctctatt cgtatttaga agctagaatg
     6961 aacgcggtta cgcaatcact ccagcctctt ctttatttct ctttattcat ttttttcaaa
     7021 aaaagattga cgctgatggt atcgattcat tcctaactgg atcagttctt caatcaaatc
     7081 accgtatttc aagcccatat tttcccataa gagtgggtac atactgaact ccgtaaatcc
     7141 tggcatagag tttaattcat tcaggaataa ttcattttta tttgtcaaaa agaaatcgca
     7201 ccggctcaat ccgcttccac ctaacatcgt gtaagctaac ttcgcgtact cttgcgcttt
     7261 ttgataaact tcttctggca cttcggctgg aatctgcatt tcgattttat tattgatata
     7321 ttttgcttca taatcataga atgctacgtc ttttacgact tcaccaggca aagtcgtccg
     7381 aacgtcttca tttcctaata cagcaacttc gatttcgcgc gcttcaattc cttgttcaac
     7441 gattgctcga gaatcatact gataggctgt tgctaaagca ttttgcagct cttctcggtt
     7501 ttctgctttt gtaatgccga cactagaacc catattcgcc ggtttgacaa acatcggata
     7561 aagcaaagaa ccttcacatt gatcaaatac ttttttagga ttttctttcc attgattctt
     7621 aagtactggt acataaggaa cttgcggcac accagcagct tgtaaaatat acttggtcat
     7681 gattttatcc atggcacatg cactggtcaa tacgcctgcg ccgacataag gcatattcaa
     7741 tgtctctaag aagccttgga tcgttccatc ttccccgttt ggcccatgta aaactggaaa
     7801 aacgatggct ccttcttctt tgatttcgcc cggattgatc acttttcctg taaagccttc
     7861 ctctgtctgt ccacttgggt cccatgaaag atgcaagaca tctttgctgg caggtttttc
     7921 tgttagtaat ggacctttga cccattgtcc ttcttttgta ataaatacga gttgaacttg
     7981 gtaataatta taataaatgg catttaaaac tgaaaatgcg gaaagaatgg acacttcatg
     8041 ctctgcgctg cgtccgccat atagtaaagt aatcttcaaa agattgtcct cctgtattct
     8101 aaatcgttca gtgatttact ctcatatttt agcacaaaac aaaaataaac gaataactta
     8161 tttataaaaa aacgagccag cttctaaaag aacgagcgtc atttattttt tgtcagctag
     8221 attatttgtc ccgtttttta taaaaagtta tgataaagaa aaggagggat gatgatggcg
     8281 caaacaaatc cacgcagccg cttggcagaa atcattcgag tgttcatcaa ttacaatgta
     8341 gtgccaaact ttgtacagca aaaaaatccc gaacaggtaa aaaaagcctt tgaagaacta
     8401 ggccccacct tcatcaagat aggacagatg ctttctgttc gagaagattt gctttcttct
     8461 gccttcactc aaacattcaa aacattacaa gatagtgtac ctagcgacac attttcaacg
     8521 gtcaaaaaga cgatcgaaac agaactttct ctttcattat cagatatatt cgatgatttt
     8581 tcaaaatcgc cttttgcctc tgcttctatg gggcaggctc atcgtgccaa gctgaagaac
     8641 ggtgacgcgg tagtcgttaa gatccaacac cctaacatcg ccgaagagat ccgacttgat
     8701 cttcaactat ttgaacgagc gatccctttg atcaaataca ttcctgaaac tagcgtagtt
     8761 gatctaaaag gggtactcca agaagtaaaa cgttccttga tcaatgaaat ggacttctta
     8821 aaagaaagtc aaaatggcga acaattctat caaaagaaca atggctggaa agagatccgc
     8881 agccccaaga tttatgacgc tttttgttcg aaaaaagtga tcgtgatgga agaaatgtct
     8941 ggtaaaaact tgaatcattt aatgaacgcc gaaaacaaaa ctgaaacttt tatcactgga
     9001 attcaaaaca agcagctgaa acaagaagtt gcaaagcttt tagttgagaa ctttatgaaa
     9061 caggtgtttg atgatggttt tttccatgct gatcctcatc cagggaatct gctattccat
     9121 gtactaacaa aagaagaaca gacacaagct agccgaaaaa ctgaaacagt ccatgaaaaa
     9181 gagttcggtt cctttgcttt tcgagcctcc acttctgcag aagaccctgt tgcgccatat
     9241 accatcaact acatcgattt tggtatgatg gggcatcttt ctgcaggact taggcaaaaa
     9301 ctcacacaag ccgttttagc gctgtatacg aaagatgctt atcgaatcga aaaagcggtc
     9361 cttcgacttt gtcagcaaga aggctcgttt gatgaaagtc ggttccacca agaactgaca
     9421 agttttcttg aacagtatta tgattctcct attgatgaaa tcaacttgca agaagtattc
     9481 acacatgtcg tgacgatttg tcatcaaaat aatctgcagt tcgatcgtga tatcacgctt
     9541 ttgctgaaag catttgggac attagaaggg gtgatccgcg tcctcgatcc tgaagtttcc
     9601 ctcatggaag tcgcttcgcc ttttgctcaa cattattttc tcactcatct ggatgtagaa
     9661 gatacattga aacaaagcgg attagactta ttggaaggga tgaaggctgc gcctaagatc
     9721 cctcaacagc tccaccatct attagaaatg tggacttcgg gacaaggaaa agtcaatttg
     9781 gaattgaaaa agcaagacaa attattgagc aggatcgaaa gtatgatcaa tcggcttgtt
     9841 ttcggtatga ttttagctgc attgatcgta gggtcctccc tgcttgtcca agcagcacct
     9901 gtagaaaacg cagaggtcgt ttccttatta gggatattta cgtatgctat tgccgcattc
     9961 gtcattattt ttttggcaat cgatgcattg atccaaatgt ataagaagag gaaaaagtag
    10021 aaaaaggttg tgacagaaag aatcagcttc aagaaataag aaggggtttt acataaattg
    10081 tttctcaaat ttttgtgaaa attcggctta tttttcgaag aggctacttc tgaaacaccg
    10141 agaataaaaa atgtgaaaaa gctgttctgc acccagtaat aagaacaatc aaaaagggaa
    10201 catggaacac atttatcctg tttccctgtt ccctttttga tgtattcgag cataaataaa
    10261 ataccttcgt tacctactat cttctgtact tggtaaacct tagtcaattg ccttttttaa
    10321 cgcttgacga aaagacatat gatctttctc cgtaaagggt tttggaccct ttgtccttat
    10381 gccagctttt cttaactttc cactatgata acgttgttct agaagcgtgc cgatattcgc
    10441 ttcgtcaaat tcttttccag agtgatggaa cactcgcaca cccttcggca atacaagtga
    10501 agctaaacca taatcttgcg tgatcaaaaa gtcattcttc ttaaccatac cgacgatttt
    10561 ataatccgca ctatcagctc cgcggtctac gtagacgatt tggacatgat tagcgtattc
    10621 tttggtcgta taatggtcca cactggtcac gataaccacg tcaagaccat aggattctcc
    10681 taattttatg acatcttctt ttactggaga accatctcca tcaatgaata gcctcattct
    10741 ttttcgcctt cttccataaa tgataaacag ttactaatcc gctcagtaaa agaccaacat
    10801 tgatccattt tgccaagata agcagttgta cactttctat cggcactgat tcatggttca
    10861 gcgtgatcaa taatagatgg attggctctc ccatttcttt tgctaaaaca aacaaaagcg
    10921 tcatcaataa aaactggtgg atcacactcc ctttattttg agtagtatac ttgacaacga
    10981 tccggcgatc cattggaata gctgtatcag tttgataata ttccttattt agccagctgc
    11041 ctgttactac gtgccagaaa ctaagcagaa agacacctat cgcaaaacca tttggaatca
    11101 agaggaccaa taaagaagaa ctcagtccaa gcaattgcat agccaatgga gaccatgtta
    11161 aacgtttcaa aatccatggt ccgactattt ttgctgcaat caagcctgct gcataaggca
    11221 aatacataaa aatcgaaagt ttttctaatc cgtacagtat gccaacataa aaactgctaa
    11281 ataaaaaaag gaaattccct agcattccat ttagaaaagt aaacagattt agccagcttg
    11341 gcaacttcca ttccttcttc tggtgtccta aaacaaacac gaacaagatg aacagcagca
    11401 agaaaccata aatagcaaga tcaaaaagcc tttcatcgaa taacaatcgg gcatttctca
    11461 aaagaaacaa taagccaaag aatatcaaaa ataaaacaag ctctcgcgta gctaccagat
    11521 ttcttttgat gtctttgaaa tccagttcgt aacgcggata atgactaacc gtatggtagg
    11581 ccatgacgta aaaaagtgta taaatagtaa agcttgctgc aatctgagtc ggtgcaggca
    11641 actcaatcgc ttgatacagg agaaatagcc agatagctgc aaataagtac tttttcccat
    11701 ccatattgat aaaaccttgc gtcttttcgt gaaaattgac cgtggtatta gcaggtacaa
    11761 gccatgctgc actcagccct agcaaaacac ttgaaaaata aaataaagga aaataaaata
    11821 cgcctaatat tccaagcaat gaaccagcac caccaatcag cagagcgatc atcagcaacg
    11881 tgtaactatc tagaccaaat cgtagactgt tgactaaaaa tagtccagtc atccgaaagg
    11941 cataaaacag cgtaaatggc aaaatcgtca gccaattatg cgagtctgtt agtcccaaaa
    12001 ataaaagata aggaataaaa ataaccgtgt tacttaagat aaagggcgtt gtttgtgtaa
    12061 aattacgccc aaatcgtttg agtttttcga acatgacaat ggctccttaa acagtcttat
    12121 aactaatctg ctcttcgatt ttagcattaa aggggaaata aaccaatttt acagaatata
    12181 ttcttgtaaa aaaaaataca gaaagaagca taagcttctt tctgcatttt tacggtactt
    12241 gattactcaa gcagacaagc ccttctccac aaaagtcttg cccgacaccc ctgcatccgg
    12301 caggtgataa agatagaaat caattatcct gtgcctccaa aatcaattag gagctctgta
    12361 aaccagagtc cttctcgacc cgtcgcataa atttacttta acacagttcc tatgtttatg
    12421 cgagagcttt tttcatttta taatcgacca tttgaaatcc ttgaccgata tctgaaataa
    12481 gttcttctgt aatctcaaat ccttcatgct tataaacctc gattgcttga tgattcccct
    12541 gattcactcg caaaaagatc tctctttcct ttgtttgcgc agcaagttct tcataccagc
    12601 gaaaaactga agaagtcaat ccttgaccac gtactttatt tagcaaatat aatttactta
    12661 aataaatttt tccatttatc tcttcataag cagtgtaacc gacagtcttt tcttcgcttt
    12721 ttaataaaaa gtaggacaca ccttctgtca gttctttttg aatctgtttt tttgactgat
    12781 aagttttcag catgtaatcc acttgttttt gaccaatgat cggcacaaaa gcttccggcc
    12841 atacttcttg tatgactgcc atcagctcat ccagttcatt ttctgttact tttatttttt
    12901 ccatacaccc acgtcctttt ttacattata aatgaccttg cgcttgatgg aataaaaaga
    12961 gcatgatcat gctatacaca cccaccgacc atggtttcaa gaaaatcaca atctgcataa
    13021 attccctaaa agataatttc gtcaaaccag ctactagaca gatcaagtcg tctggcgcaa
    13081 agggaagcag caaggtaaca actagtactt ttttaatatc tttcgttttt cttgtcagca
    13141 attcatcaaa tttctgatac ttttttggcg ataaaataag ctggacaaag gctacgccat
    13201 agtaacggac aagcaaaaat ccgattgctt ccccaatcac taggccaata caactatata
    13261 aaagcccaaa tccattgcca aacatcagca tgcccgctac tgaggagatc cctcccggca
    13321 aaataggaat gatcacttgg atcacttgca acagaacaaa tatcatcact gcatacttgc
    13381 caaattgctg gataaacgct tgcaaagacg tgctcgatcg aaaaatccca tgatgataag
    13441 cataaagaat caacacaaag aaaaaaaaca gaccaataat gggcatgatc tgaagacatt
    13501 tttgtaaaac tgatcctttt ttagacatct atattcccca catctttttt catttattaa
    13561 aactgcctcc t
//
LOCUS       DANKKG010000065        13559 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000065 DANKKG010000000
VERSION     DANKKG010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13559
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.065"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            202..1389
                     /locus_tag="PW004_001629"
     CDS             202..1389
                     /locus_tag="PW004_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDL2726603.1"
                     /translation="MQQLSQNSLFLKTCLLYLEEDEPDYLQLTECEFISVSKAYSLKK
                     QVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRLGGFNSWELPESFFERADRFIES
                     VTENSGRFYDKENKEILSIGFAISFLRQQVCAVTIDSKFIEEIKKRPIYNYVESAWEN
                     TDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDFQQLHQVFIDNTPEIKELVATFE
                     SHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKFYLLNEEQTNLYKEVQTIFSSWS
                     SQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYIVTNSDVHYLIYREALEAVTTFD
                     FQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDAVQYENIIPISINTIDRAIISAV
                     QNK"
     gene            complement(1481..1846)
                     /locus_tag="PW004_001630"
     CDS             complement(1481..1846)
                     /locus_tag="PW004_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HDL2726604.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1843..2208)
                     /locus_tag="PW004_001631"
     CDS             complement(1843..2208)
                     /locus_tag="PW004_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HDL2726605.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2400..2648)
                     /locus_tag="PW004_001632"
     CDS             complement(2400..2648)
                     /locus_tag="PW004_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726606.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2647..2775
                     /locus_tag="PW004_001633"
     CDS             2647..2775
                     /locus_tag="PW004_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HDL2726607.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2832..4526
                     /locus_tag="PW004_001634"
     CDS             2832..4526
                     /locus_tag="PW004_001634"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HDL2726608.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4530..4649
                     /locus_tag="PW004_001635"
                     /pseudo
     CDS             4530..4649
                     /locus_tag="PW004_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            4626..4922
                     /locus_tag="PW004_001636"
     CDS             4626..4922
                     /locus_tag="PW004_001636"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726609.1"
                     /translation="MQAFEHRLPRGCEKAALHQVIRTPKLKLLLMFLTWLDLLPQMQA
                     FEHHLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQAFEHRLPRGYEKGV"
     gene            5300..6433
                     /locus_tag="PW004_001637"
     CDS             5300..6433
                     /locus_tag="PW004_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HDL2726610.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6464..6784
                     /locus_tag="PW004_001638"
     CDS             6464..6784
                     /locus_tag="PW004_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HDL2726611.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6813..7277)
                     /locus_tag="PW004_001639"
     CDS             complement(6813..7277)
                     /locus_tag="PW004_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2726612.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7408..8661)
                     /locus_tag="PW004_001640"
     CDS             complement(7408..8661)
                     /locus_tag="PW004_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2726613.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8690..9007)
                     /locus_tag="PW004_001641"
     CDS             complement(8690..9007)
                     /locus_tag="PW004_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDL2726614.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(9033..9353)
                     /locus_tag="PW004_001642"
     CDS             complement(9033..9353)
                     /locus_tag="PW004_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2726615.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9817..11232)
                     /locus_tag="PW004_001643"
     CDS             complement(9817..11232)
                     /locus_tag="PW004_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL2726616.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11249..12565)
                     /locus_tag="PW004_001644"
     CDS             complement(11249..12565)
                     /locus_tag="PW004_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2726617.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12715..13413)
                     /locus_tag="PW004_001645"
     CDS             complement(12715..13413)
                     /locus_tag="PW004_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDL2726618.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
BASE COUNT         4291 a         2766 c         2336 g         4166 t
ORIGIN      
        1 ctgtttttat gtgggcaatt ctatgtaaat aaagaagtca atctagaaaa gctctccaat
       61 ctcctgcatg tttgcaaaac cactttactc aatgatatca ataacataaa aaaagaattt
      121 gaagaacaga tcgcttatac acatcgggaa aaggaccgtt acactttata tttttcagaa
      181 catatacctc gatgcaagat catgcagcaa ttgtctcaaa attcgttgtt tttaaaaact
      241 tgtctgcttt atttggaaga agatgaacct gattatcttc aattaactga atgtgaattc
      301 atctctgtca gcaaagctta ttcattaaaa aaacaagtat tagcttattt taatgactgc
      361 gggatcgaaa tcgaccgcta ctcccctcga ttcactgaaa tggaaagacg tctgctgctt
      421 cttaatgtgt cgtatcgttt aggcggtttt aattcttggg agctacctga atcgtttttt
      481 gaacgtgccg atcgtttcat tgaatccgta acggaaaata gtggcagatt ttatgataaa
      541 gaaaataaag aaatcttatc gattggtttt gctatcagtt tcctacgcca gcaagtgtgt
      601 gctgtgacaa tagattcaaa atttatcgaa gaaatcaaaa aacgtcctat ttacaattat
      661 gtcgaatcag catgggaaaa cactgatttc caaacctatt ataaaaaaga agaattcgcc
      721 tttattttga cactattcaa tctttgtaat tatggtttcc attcttatca gttgattgcg
      781 gaagactttc aacagctaca ccaagtgttt attgacaata ctcccgaaat aaaagaacta
      841 gttgctacct ttgaatctca cttcaatcaa gaattattcg gaaaccaacc gttcgaacgt
      901 gcgctgattc atctgatgcg ttcggcttgg gataactatc aattattcat gccagaaaaa
      961 ttttatcttt taaatgagga acagacaaat ttatataaag aagtccagac aattttttcc
     1021 agctggtcca gccagttacc ttatgatcta cggttgaatc ctaattgcat gcgggctttt
     1081 gtgatcgaat tgagcggaat tctccgtctg acaaaagaac atctgactat ctatatcgtc
     1141 acaaattcgg atgttcatta tctgatttat cgggaagcct tggaagctgt aactactttc
     1201 gattttcaag ttgcaccaac aatttattca agtatatctg atataaagaa gtatgcgcag
     1261 cagtcctcaa accgggtatt atgtgaacgt acattatata caccagatgc ggtccaatat
     1321 gaaaatatta ttcctatttc gatcaatacg atcgatcgag ctatcatttc agctgtgcaa
     1381 aacaaataag gggctgtgac aaaaatcatg tcacagcctc ttatttgttg aagatgccca
     1441 tttctgtaac aatctcttga tacacgtcag gctcacaacc tcacttattt tggacatcta
     1501 cgggtccgat ctctttattt aaaaaataag aaatgatggt tcggataact accaacgccg
     1561 ctagttggaa aatatcctgc aaggtaggtt tgataatcga ttcgatgatc ccagctgaaa
     1621 caagaatttc caaacttaga agtatatagc ctcccaacat ctttttaata gcattgttct
     1681 cgctgactcg gtcgctttgc acttttgtat ggacaaataa atatttcaaa aaatccttga
     1741 ttgatagaca aacaccccaa accaataaac agatagcaaa gatatcaagt cccagtacaa
     1801 gtttttcgaa atatggaaga agaaactgca tcaactctgt cgtcatccta cctctcctct
     1861 tctttgtttt tctttgtctt ctatttcctg attcaagaaa taagaaatga ctgttcgaat
     1921 gataataata agtgcaagct tcaaaatatc tgtccaagtc ggcttgataa ctgactcgat
     1981 gatatcagca acgactagta cttccaagct taacaaaata taacttgcta gcagttttct
     2041 tgtatctgca ttacgagtat tacgttctgc atagtttttc ttgacatgag accaatcaaa
     2101 aagtttgaat agaacaagaa cgatcccgac aattaagata ccgatagaac aaatatttaa
     2161 aatagcaatg atccattcta gaaattcaat taaatttgag cgaaacatat acgctaccta
     2221 ctttctttta atttgttcct attcttttat tgtaaaagaa agttaccttg ttcgtctatt
     2281 taaaagttgt tctaatgata acggttcgct taaatttttc tttttcactc tcccattttt
     2341 ctccctccat ccataaatcg tggaagtatc caaaacaacg tagcaagtta aaataaggct
     2401 tagtccgtca tcagcttttc aaagaagcac tgtctcggtg ctttttttgt acccaaattt
     2461 ctaaaaatag aaccatacac ctcctcaaat cttttcttct tacctaccct atttttattt
     2521 acaggaaaat cagacacttc ttcttttttg tcaaaatttc ctttttcttc tcgtcctatc
     2581 atttggcatt ctaataaaaa cgaactatcc ttggatatag aaagagaaaa caaggaggcg
     2641 tttttcatga gtaaaaaatt aatcaaagtc ggcatcggtt taggtttgct tgcccttggc
     2701 gctgcatatc taggaaaaaa gacaggacta ttcgaagatg acagtcattt atatgatgaa
     2761 ttcgaatcaa tctagcatcc tatttagttg aatcactacg gacatacttg gaagacattg
     2821 gaggaatcac aatgagaaaa agtaaagcaa taatgattgg tgccggtatt gccaatatgg
     2881 cagcggctgt ttatttgatt caagaaggca aatggaaagg ggatcagatc accttttatt
     2941 cattggatga tcacggttca aatgatggtg caccgaccga cactgtagtg gatgaatatt
     3001 ggaataaaaa ccatccatta gaaaaccaaa aaggctatat tgctcgaggc ggtcgcatgc
     3061 ttaattaccg tacctatgta gatttgatgg atctgttaag tcgaatacct tctgttactg
     3121 agccagggat gactgctgaa gaagatacga gagattttga tgcaaaacac cgaacctttg
     3181 acaaagcacg tttgatggaa ggtggcaaag gaatcattga tggcggaaaa ctgggattca
     3241 ataataaaga ccgtattctt ctgactaaac tgattgctat gcctgattca gaagaagaaa
     3301 aactagacaa tatcactatc gaagaatatt tcaaagatga tccacacttt tttgaaacaa
     3361 acttctggtt tatgtgggaa acaacatttg ctttcagaac acgtagttct gctcaagaat
     3421 tgcgtcgcta tatgcatcaa atgatttatg aattcactca aatcgaacat ctagtgggcg
     3481 tcaatcgaac acgttacaat caatacgaaa gtattatgct tcctttgatc gagtacttga
     3541 aagaacaagg atgcaaaatc attttgaacc gccgtgtcac tgactgggaa ttcaaagaaa
     3601 caccgatgca agatgaaatc actgtgactg gtttgaaaat taccaataca ctgactcaag
     3661 aagaagaata cgttgtggtc gatgaagata cagcagttat tttcactaat gggtccatta
     3721 cagattcagc tactttagga gattacgaaa caccagctgt agaaaatatg gattatggtg
     3781 cagcttctag tctctggaaa aaagccagtg aacgtttcta caacttggga aatcctgaca
     3841 aattctttgc tgatcgagat gcaagcgaat gggtcagctt taccctgacg acgaaaaacc
     3901 atttgttgtt gaatgaaatc acccgtatca cgactcaaga accaggaaat gccttgaatt
     3961 catttatttc aaccacaccg atcacgccgt taggccaaaa agatgtcaat atgtctatcg
     4021 tcgtgcatca tcaaccgcat tttacgtctc agaaaccaaa tgaaacagtt atttggggtt
     4081 atttccttta tccaagacga cgtggcgaat ttgtggataa acagtatatt aaaatgaatg
     4141 gaaaagaaat gctagaagaa ctgattggac aactttcaaa agtagatcct ggaccagtga
     4201 atattcgtga aaaagaaaca gaaattttcg acagcgtaat aaataatatc cctgtctata
     4261 tgccttatgc ttccgcgttg ttcaataatc gtgccaaaag cgatcgacca aaagtcattc
     4321 ctaaacattc gaccaatctg gcctttacag gagaatttgt cgagcagcct tatcagatga
     4381 tctttactga acaaagtgct gtacgttctg gagaaatcgc tgctttccat tttgcgggta
     4441 ttcctatgtc ccgtcttgtc aaaacaccgc gttacgacaa agatattcct actctagctc
     4501 gtgccgcaaa aaaaatgttt gaataatcaa gaggttgtga aaaagctgcc ctgcatcaag
     4561 taataagaac aaccaggcta aaacagcttc ttatgttttt gatttggttg gacttattac
     4621 cgcagatgca agcttttgaa caccgtttac caagaggttg tgaaaaagct gccctgcatc
     4681 aagtaataag aacacccaag ctaaaactgc ttcttatgtt tttgacttgg ttggacttat
     4741 taccgcagat gcaagctttt gaacaccatt taccaagagg ttatgaaaaa gctgccctgc
     4801 atcaagtaat aagagcagcc aagctaaaac agcttcttat gtttttgact tggttagact
     4861 tattaccgca gatgcaagct tttgaacacc gtttaccaag aggttatgaa aaaggcgttt
     4921 agtttcgagt atgaagaaaa attttgtgaa aatggttcct cacgtttttg cataaatttg
     4981 acttaatacc gagaaactga tcccttctgt cataacctct ttttatttat gtctgtttct
     5041 ttttgaaaac aactattttt tcaacgttta ctctcttgcc aatccaacat catctaacgg
     5101 atacaactat ttcaatgatt accattacac taaaaaatcg gaaacacacc tctccgaaca
     5161 caaatacata agactataga atgtttcatt taagaaaaaa agcgtttcat tatcaaaaag
     5221 gtaagatctc ctttaaaatg aaattatctg tagtactaat aatgcagaca attttatgaa
     5281 tctgaaagga gcatctccaa tgaaagcagt cacttggcaa ggaaaacaga aaatggaaat
     5341 cagaaacgta gatgatccaa aaatattaga accaacagat gcaatcatcc gtatcacagc
     5401 aacagccatt tgcggctccg atctgcatct ttaccatcat ggtgaagcag ttttggaaaa
     5461 agggtatgtt gtaggacacg aaccaatggg aatcgttgaa gaagtgggat ctcaagttca
     5521 aacattgaaa aaagggcaac gtgtggttat cccttttaat attagttgtg gtcattgtca
     5581 tttctgcctg aatcacatgg aaagccaatg tgatgaatca aatccagatc agttatatgg
     5641 cgggcttttc ggatttggca agttgaacgg taatcattgg ggtggtcaag cggaatatct
     5701 aagagttcct tatgcagact ctacttcatt tgttgtacca gataatgatc ttcctgatga
     5761 aaaagtttta tttctatcag atattttgcc cacagcttat tggagcgttg aaaacgcagg
     5821 agtaaaaaaa ggcgatacag ttgtgattct aggttctggt ccagtcggtc tattcgctca
     5881 aaaattcgct gttatggctg gagcagagcg cgtaatcgca gtagatccag tacagcaccg
     5941 tctagataaa gcggctaatt acaatggggt tgaaacttat ttcttagaag acacggcaca
     6001 agctggaaca gaattgtatg aactggctaa aggtggcgca gatgtcatta tcgattgtgt
     6061 aggaatggac ggattagagc ctgtcaaaga aaaagcgaaa aatttagtaa gcttacaaag
     6121 cggcacaatc tctccattac agatggcatc gcaagctgtc aaaaaatttg gaacagtcca
     6181 agtaactggt gtttatatga ctccagcttc ttcttacccg ctgcaagaat tttttatgcg
     6241 ggatatcgct gtcaagcatg gacaggcacc aattatccat ctaatgccaa aaatttacgc
     6301 aatgattgca gaaggactat tcgatcctac acaaatcatt actcattcgc tgccactatc
     6361 agaagcagca caagcatatg agatctttga caaaaaagaa gataaaaata taaaagttgt
     6421 tttgaaacct tgaattcagt aaaaaataca ggtgattttc tttatgccca aaatcaaacg
     6481 aaatacccat gagatcgaaa taggaaaaga agaagatatc gaaatacgag gacgtcgctt
     6541 ccgcttctat ttccaaaatc gttacacatt gatctcactg gctgtcgatt tattaaccgg
     6601 gattttctat atttttggaa gccttgcttc cttaactaaa atccccgatc gatatggcac
     6661 ctatttttat ttggctggtg ctattttctt gacgattcgt cctattctca gaatcgtaag
     6721 gaacattttt atttacgacg aaaaaaaagc aatgaagaga acaagcaaaa aaaaagaggc
     6781 atgagaattt ttcaaatctc atgcctcttt tttcacccta tcgtatcact ttgatacaga
     6841 cgagcggtcg tcccactttc ttccttgacc attgtcaaaa atgtccactc gtatcgttca
     6901 taaaacgtgg tatgatctgt aattaagtaa agtttttcgt acccaagttg atgcatttct
     6961 tttctgattt ccgctaatat ccgcttggct atcccctgct tacgcttttc cggttcaaca
     7021 aacagggcac aaagatttgg tgtcagatca gggcggttat ggaagtcgtt ttcaatgacg
     7081 cccgctcctc cgacgatggt ttctttttca tctaagacca cataccattg aggaatactt
     7141 tccttattag cgattccagt acgaatactt tcctcataca caacagcagg aactccccat
     7201 tttgatgaaa accaattcgc gctttgtttt actaattctg ggtgttctct taatttgatc
     7261 cagttatatg taaccaatgt ttaaacctcc gactaaaata aaacgtttac aaaaattata
     7321 caagtcttta tgaaaattag caatgacaac aatagtgatg taaaaaaaaa acggaaaaca
     7381 atcacttgtt ctccgttttt tgattaatca agcatctaaa gcttcttttc ttctttcttg
     7441 gatcgtagct accttgacga acggtagata gatcaaaata gaaagaacca aattgacagc
     7501 ggctagtaca ccacctgcaa tactttttgt tgccaataca ccgccaataa ttggtggagt
     7561 tacccatgga ggcataacgg tagctgctgg cactaaacct gtagaagtcg caaagtaagc
     7621 aacactaact aagaccattg gtgtcaaaat aaatggaatg aacatgatcg ggttcaacac
     7681 gataggtaac ccaaacatcg ttggttcatt aatattgaag acacctggcg caatcgacaa
     7741 gtttgttaca accatatatg gtttgttttt acgtcctact aaataaatag ctaacagtaa
     7801 acctaaagtt gcacctgttc ctcctaagtt gacaaagctg tcaaagaaag gtttgttcac
     7861 gataaacggt gcgctatgcc cagctgcgat tgcattaacg tttgcttcaa tggctggcgc
     7921 attgattgtt tgcatcaaag gatcgaccat attcgctccg tgtaaaccaa agaaccataa
     7981 gaatggtgtg ataaatgcaa gcagtaaggc agatggataa gagtttgcaa gtcccataaa
     8041 tggttcttgg accactgtat agaaagaacc tacgatatca tcaacaccga atgcagcaaa
     8101 aattgcagca actaaagcaa acgcagagat agtgatcatt gctggtagta atgaagcaaa
     8161 tgcttttgca actgccggtg gcacaccatc aggcattttg atgatcaatt tttcatttcc
     8221 gcttaaacgt acaaagattt ctgttgaaat aatcccaaca ataatagcaa taaacaaacc
     8281 gtttgagctc atacctaatg ctccgcctaa agcgaagaaa gagcctaaag aaatcacacc
     8341 tgcggaaaca ccgtcttttc ggtagccgtt agctaaatga taagcaagca agaaagcaat
     8401 caagacagaa agaatggcaa atgtaccgtt ccaagcagca gccccgaatc ctttccatga
     8461 ttcaccacca aaaattgagt tcatcaattc ttggaacccg ggaatcggta agttattgat
     8521 cattactgct aatgctccta agatcattaa cggcatactc accataaatg agtcccgaat
     8581 cgcaactaaa tgccgttgtg caccaatttt tgatgctaca ggtataaaat gtttctccat
     8641 aaatgcaaca aatttatcca taaatcttcc ctcttttctt tactgtagat tacgcgttca
     8701 atcgacgata cagatcaatg atctcttttg ctaaatctgt aaaagctata gatgtcatca
     8761 gatgatcttg actgtggacc gtcaaaagcg tgacttcaac gtgatttcct tgtgcttctt
     8821 gtgtcagcat ctctgtttga gagtgatgtg cttggactaa agattcctct gcatctttga
     8881 ttttttcatc tgctaaagtg aaatcacctt ttttcgcagc agcaatcgct tccattgcat
     8941 cgctttttgc atttccgcca aacatgatca gacccatgat ggcttctaga tttttttgat
     9001 cttccattta ttgtatcctc cgataatttt tattatttgt ttattagttt ttctgcttgg
     9061 tcgagtactt tttcaccatt catcatgcca tagtcagcca tattgattac atctaaagga
     9121 atgcctttag gtgctagctt ttcagcaaat tgattttgca taaagcgtac ttgcggtcct
     9181 aaaagtaaaa cgtctacatt tttagattct agttgattat ccgcatctga agcagataca
     9241 gcaaagatgt ctgcttccat tccttttgct tctgctgcct tttgcatttt tgtgactagg
     9301 agacttgtac tcattccagc tgaacataca agcatgattg ttttcttttc cattttaaac
     9361 acgttccttt ctatacttac acttgtatat aagcaagtag cgtgccaact ttaaaagcgc
     9421 tttaaaaagt tgatatcatc acatttaagg tctataccaa gttgcataca cactaaaaaa
     9481 gaaagcgcac actaaacaca caaattaatt tttattacct tttttctttt aacgtgcaca
     9541 aaaaaagttg tgacacaaaa cttgtcacaa cttttttcaa ataaacggta ttcaaaagct
     9601 tgcatctgcg gtaataagtc caaccaagtt aaaaacataa gatgttgttt ttactttgct
     9661 attcttatta cttgatgcag gacagctttt tcacaacctc tcaataaacg gtgttcaatc
     9721 agctgaccct gggtattagc tcaaaacgct ggattcacaa cctcttttat ttcaaataac
     9781 cagtaaatac aatgaacatc tacaaaaaac ttctatttat ttatagattt cgtcaggcaa
     9841 atccaattct ctcttatcaa tcaaagtctg ataccacgaa gcagattttt tcaatgttct
     9901 gcttcgttca tcgttcaatt cgattcgaac taatccatat cgattcttaa atgcattcat
     9961 tggtgacaca cagtcagtga atgcccataa catatagcct tcgcaatttg cgccttcttc
    10021 tgttgctttg atcaagtaat gcaaatgctg tccgataaat tcgatacggt aatcatcttg
    10081 cacgattcct ttctcatcca tatactgttc ttcatattct cttcccatac cgttttctgt
    10141 tatcagccaa gggatattat gataattttc ttttagatac atggctatat catacatgat
    10201 tttcggatag atttcccatc ccctggaatt attcattttc ttcccaggta aatcaaagac
    10261 ttcataatat cgttcaggat ggaaaggtgt ctcttcattc cattgataac gaggggcgcg
    10321 aacgcgttta ggataatatt gattgattcc aaggaaatct actgtatgat gctgaatcgt
    10381 ttctaaatcc tctgttgcag gatcgaaata aatcgattgt ttttgacaca actccaggaa
    10441 ctcttctgtg tagtatcctt ttaccatcgg atcaaaaaat actcgattat aaaacaaatc
    10501 atacatataa gcagcttttt ggtcttctgg agaattggat cgagcataga ccatttcagg
    10561 atttaaaata cagccaatcc ttccaccata atttccgctg cggtaaatct ctactgcttt
    10621 agccgttgct aaaactttat gataattcca catcatccac ttttttgtgt tttgttcatg
    10681 cggccagcga acagcatcca aatagcaacg cgtttgggga acgattggtt cgttgaatgt
    10741 aaaccagttt tttaccctat caccgtaacg atcaaaggca atttgagcat acttagcata
    10801 aaaatcaact acttttcttg aagaccagcc atcatatttt tccaaaagaa aagctgggac
    10861 ttcataatgt tccaaacaaa tcatcggttc tacgtctgct tcgatcaaag agtcgatcat
    10921 ttcacttata tgctgagcat attcttcatc tactatacag ttctcataat cagtaaaaaa
    10981 acgagcccaa ttgatagaag tccgataatg tgtcagatgg atggatttca tcagatccac
    11041 atcctgctgg tattgttcca taaaattagt tgctacggct gggccatacc cattatgcca
    11101 aacaaaatgt tcatttttat accagctatc tataaacgaa tcctgtcctt ctttttttcc
    11161 tttccaacct tccgtttgcc aagcagaaga tgcagcacca agaataaaat tttttggaat
    11221 agtaattttc atgatttcct cctgtttttt agatcccatc attttccaca cgatttgcta
    11281 cttttacaaa tggcagatag atcaaaatag aaacaataat acaaacaagt tgcgtaataa
    11341 ctgcaccgat atttcctgct gttgccagat aagcattgac gatcggcggc attggccaag
    11401 ggaccattac aactgctttc gccgcaaatc ctaaagcggt tgcaaaataa ccaatcgttc
    11461 cagtgataag aggcgttaag ataaacggaa tagctaaaat tggattcagc atgatcggca
    11521 taccaaaaat aactggttca ttgatgttga agatcccagg agcaagggat aacttggtga
    11581 tttgtttcat atcttcccgt tttccaacta acaatactgc aatcagtaaa ccgatcgtga
    11641 ctccagaccc gcccatactc atatacatat cccaaaatgg catagtaata atgttcggta
    11701 cttcttttcc agcttcaaaa gcttccgtat ttacagcaat tgccgctaaa agcaacggtt
    11761 cccgtatcgg cttcaccatt tgatttccat ggataccaat cacccaaaag acttgcgcaa
    11821 taaacatcaa tagcaaaata cccggtaacc cttgtacgac accttctaat gggcgttgta
    11881 cgacattgta aatgatatca taaaggtaca ttcctgtaag ttctttgatg ataaatcccg
    11941 cagccgcgat acccgataca gtcagaatcg tcgggaataa agcagaaaaa ctagcagaaa
    12001 cattcgttgg cacggtatca ggcattctca gctgtagttt cttctgtttc ctcaaccagc
    12061 aataaagttc aatagaaagg atggagacca acattcccaa aaataaccct ttcgtatctg
    12121 tatattgttt agccaatacg ttttctacta taacagtctg attttcattc actaattcat
    12181 aaacagttgg attaacagat aaataagaca tcactgcgat gataccaggg aatacaccat
    12241 ccactttatt cagcttggct aattcgatcc ctatcagaaa aacagcatag atggtcaaga
    12301 aacttagtgt gacatagctg atggcagatg cgataggctt taattcttct aaaaaacgca
    12361 agaaacttat ctgtgctaag ccattttccg agtcaaagac cattgctgaa aacaatgtgc
    12421 caaaagcacc cgtaataatt accggaatca atgcagcaaa tgcgtttttt atcaccatga
    12481 tataccttaa attattgatt ctcgttgcca cgttccctaa aacattggct aatttatcca
    12541 taaaactcat tccagatccc ctcattcctg cttcttctcc ttgacgtcat ctctttttgt
    12601 ctattagtca gcgcttacat ttcgatgaac agaaactatt tactcctctt ttttaccaaa
    12661 tataaaacaa aaattcatct gatgtattta tcataaaata catcagatga actgtcaatt
    12721 agtttctttt tctcctatta ttttctgtaa ttctctccga tttattgcca tatggacagt
    12781 cattgcatca aacgcagcaa gtgcattccg cctttcgatg gcctgaacga tctctgtatg
    12841 aatatcgatc gtctcgattt tcagatgctg gatatcataa tttttattaa acaaagcaat
    12901 tgattcatta atgattggga taatatgcag caaggctacg tttccgctag cttctgcaat
    12961 catcgtatga aaatcgatgt ctaattgaac atgttcttgt gtgccttctc gaaaagaagt
    13021 ttcaatcttt tgcgttaatt ttttcatttg gacgatttga ttcttcgttg catattccgc
    13081 tgccaaacct gccatcttag gctccaataa atatctcagt tcaaataagt ctcgtatcat
    13141 ccgatcctga tcttcaatca aagaaaaacc gagtgggtca ttggcaatcc ctcgcttatc
    13201 actgacaaat gtccccgctc cttgccgaac ttcaaggata ttcctggaag ctagagcccg
    13261 cacagcttct cgcaccgtgc ttcgccccac ctctaaataa tcggacaatt catattcatt
    13321 cggcagttta tctcctactt gcaactgcat ttcctttaaa tatctaagga tcttgtcagt
    13381 agttacttct actagggttt tattttcttc caaaccacat acctcctttt catctgatgt
    13441 attttttcgt aattattata acacgtttga atcatctgat gattttttgt atacaaaatt
    13501 gattaatccg ttcatagaca tacaaacgaa gaagatattt accataaaaa agactagga
//
LOCUS       DANKKG010000066        13423 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000066 DANKKG010000000
VERSION     DANKKG010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.066"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            210..1016
                     /gene="proB"
                     /locus_tag="PW004_001646"
     CDS             210..1016
                     /gene="proB"
                     /locus_tag="PW004_001646"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HDL2726619.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            1013..2260
                     /locus_tag="PW004_001647"
     CDS             1013..2260
                     /locus_tag="PW004_001647"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HDL2726620.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            2336..2680
                     /locus_tag="PW004_001648"
     CDS             2336..2680
                     /locus_tag="PW004_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726621.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(2797..3123)
                     /locus_tag="PW004_001649"
     CDS             complement(2797..3123)
                     /locus_tag="PW004_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726622.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(3496..4698)
                     /locus_tag="PW004_001650"
     CDS             complement(3496..4698)
                     /locus_tag="PW004_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2726623.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            4815..5330
                     /locus_tag="PW004_001651"
     CDS             4815..5330
                     /locus_tag="PW004_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDL2726624.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(5529..5975)
                     /locus_tag="PW004_001652"
     CDS             complement(5529..5975)
                     /locus_tag="PW004_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HDL2726625.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(6022..7500)
                     /gene="msr(C)"
                     /locus_tag="PW004_001653"
     CDS             complement(6022..7500)
                     /gene="msr(C)"
                     /locus_tag="PW004_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HDL2726626.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(7854..8483)
                     /locus_tag="PW004_001654"
     CDS             complement(7854..8483)
                     /locus_tag="PW004_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDL2726627.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGVEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(8467..8931)
                     /locus_tag="PW004_001655"
     CDS             complement(8467..8931)
                     /locus_tag="PW004_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL2726628.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(9418..10686)
                     /locus_tag="PW004_001656"
     CDS             complement(9418..10686)
                     /locus_tag="PW004_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HDL2726629.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(10917..11654)
                     /locus_tag="PW004_001657"
     CDS             complement(10917..11654)
                     /locus_tag="PW004_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL2726630.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(11721..12290)
                     /locus_tag="PW004_001658"
     CDS             complement(11721..12290)
                     /locus_tag="PW004_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDL2726631.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLLVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12418..13290
                     /locus_tag="PW004_001659"
     CDS             12418..13290
                     /locus_tag="PW004_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL2726632.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
BASE COUNT         4050 a         2761 c         2427 g         4185 t
ORIGIN      
        1 actactccta ttctaacata tccgaggctg taaaagactt tctttacaaa ttcttcaaga
       61 aattctgcta tcttaaagta aaccattaat tcgttctttt tccaaacgac tacaagttgt
      121 acaaaaaaac tattttttac atgactttac tcgtcaaaac aagtagaata gaacgaaatt
      181 aatgagtgga tatttgattg aggtgtcaca tgcgtaaaga aattaaaaat gcaaaacgaa
      241 ttattgtcaa agtaggaaca agtacattga tttatcccaa tggaaatatc aatcttgcgg
      301 caatcgatca gttagctttt gtcctttctg atcttagaaa tcggggaaag gaagtgatct
      361 tagtctcatc aggagctgta ggcgttggta tgcataaatt acatttgtca gaacgtccga
      421 ctttgattcc tgaacagcaa gctgcggcgg ctgttggtca agccgaactg atgaatatct
      481 ataatcaaag attcagtgta tataatcaac agacggcaca gatcctcgtg acaagagacg
      541 tggtagatta tcctgaaagt cgtaaaaatg tgaggaatac attggaacag cttttgatta
      601 tggacacgat ccctatcgtg aacgaaaacg atactgtagc agtggatgaa ttagatcatc
      661 tgacaaaatt tggcgataat gatcagctgt cagccatcat ggcaaaactt gtcgcggcag
      721 accttttgat cgtcctttct gatatcgacg ggttttattc agataatccg actacaaatc
      781 ccgaggctca gatgtatcgc tatatcgata aaatcgatga aaagattctg gcacaagcag
      841 gtggtgctgg tagcacattt ggaacaggcg gtatgcagag taaattaaaa gccgcttcta
      901 ggatatttga agcaaacagc gcaatgattt tagcaaatgg aaaaaatccg gcaattattt
      961 ttgatattct tgcaggaaaa gaagtaggta ctttatttaa ggaggacttg aaatgactga
     1021 cttgatgcaa ttaggacaac aggcaaaaga tagcgcgcaa caactagcac ttatgcctac
     1081 aaaccgcaaa aatgagctac ttgttcaaat ggcacaaacc atcaaaaaga atcaacaagc
     1141 gatcattgaa gcaaatcata aagacttaga aaaagcagtc gaaaacaata tcagtgaaac
     1201 gatgcaagac cgcttgcagc tgacacctga gcgaatcaat gccatggcta cagagatcga
     1261 aaaaattgct tcgttagacg atcctattgg aaaagtagat gagatgtgga cgaacaccga
     1321 cggattgaga atcggcaaaa agcgcgtgcc actaggcgtg accggcatca tttacgaatc
     1381 tcgtccaaat gtcacgacag atgcagctag tctagcattc aaatcaggga atgccgttat
     1441 ccttcgcggt ggcaaagaag cattcttctc gaatcaatta ttagtccaat tattgcagca
     1501 agtactggtc agtgctggcg aatctcctta tgccatccag tttgtcgatg atacttctca
     1561 tgaaactgca ggacagttga tgcgattgac agaatactta gatgtgctga ttcctcgagg
     1621 cggagcaaaa ttaatccaac gagtcaaaga acaggcaacg gtacctatca ttgaaacagg
     1681 tacaggaaat aatcacgtat atatcgataa agaagcacag ttatctatgg cagtgaacat
     1741 catcgtcaat gcaaaagctt cccgtccatc tgtctgtaac gcggcagaaa cattgctgat
     1801 ccattctgag atcgcacctt ttttcttgcc agctattgaa aaagaactgg tagaacacgg
     1861 ggtctcttta cgtgcggatg ctcgtgcgtt agaatatcta gagacggctg ctttagcaga
     1921 agacacagat tgggatactg aatatctaga ttatatcctt gctgtcaaag tggtcgattc
     1981 tttggatgaa gcaattgcac atatcaatcg gcataatact aaacattcag aaacgatcgt
     2041 gacagatagt tatgcggcta gtcaacgttt cttaaatgaa gtggacgcgg cggctgtcta
     2101 tgtcaatgcc tccactcgtt ttacagacgg atcggtattt ggttttggtg cagagattgg
     2161 tatctctact caaaagttgc atgcccgtgg tccaatgggg ttaaatgaat taacttccac
     2221 aaaatatatc atttatggcg atggacaaat ccgcgaataa tgtttcgtgt gaaacattat
     2281 tcccgccttt tgcctttttt ctgtaaaggt tctatccaac tagaaaggtg tcaatatgtt
     2341 tgaaatttta atgatttttt ttatttacct tatttctctg aatatcgctg cttttctggg
     2401 agtcggtatt ttatcccttt tctttcaatt caaaaaacga agcataggga gtcaacggga
     2461 aaaatggtcg cagtattttg acaaaatcgg gcaaaaaggg ctggttacac gcttgcacat
     2521 tagttacatg gttgcattat gcttattagc gacaatcaat tactattctt ttttcgatca
     2581 ttcaatcgct tatacgatta cgctactgat tgctgggatt tttcacttat cttataaata
     2641 tcagctgaat aaaaaccact tgaatcggac tttccgctaa aaaatatccg tacgataagt
     2701 agagtgcctt gaagatcggc aaaaaactgc cggacttttt agaggctgtg acaaagattt
     2761 tgtcacagcc tctttttcca tataaacggc gttcgcctac cagctgcctg gtaaatctgt
     2821 ttcctcttcg ttaagaagcg aaaatccgtc aaatgtttcg ccatcataat aacgaagcag
     2881 ctcatccatg gacaagttca tcgttttttc tttttcttct tccgctgatt cagccagtag
     2941 ctgttcccga tacacctgat acataccagc tattttttgc tgcatcgctt ttctagtttc
     3001 accttccgca ttgattattg gcaattcttt gatatagcca cgataaatca accgatcatc
     3061 tgaaaattca atgggtaatt caacgagtgt caacccgact tcctgaaaat attcttccag
     3121 catctattat ccccccattc tttttcttaa atgaatttta gcttacctac tgaaaatttt
     3181 ccagtttcat tattttgaga aaaaaaggga atgtgacaaa aatcgtgtca catccccttt
     3241 ttccgaataa atggtgttct tctgccaact cctcaatcat aagtcacaat attcaacaaa
     3301 catgagaaac tgttttttga aattgttcct tgtgattcag agttaaacga cagtctcaca
     3361 acctcttggt aaacggcgtt caaaagcttg catctgcggt aataagccca accaaacgaa
     3421 aaatatgaga agctattttt gtttggctgt tcttatgact tgatgcagga cagctttttc
     3481 acaacctctg atttcttatt cttcttcatt tgtagcaaaa taattacggt gataaatgat
     3541 acaacataca ctgataagaa caatggcacc tgcaaaataa aacgcatagc tttcaccaat
     3601 atgttcacca atcacacctg caataaatgg tgctaaagct ccaccgaaaa agcggacaaa
     3661 actatagctg gacgaagcaa cgtttcgttg cagacctggt atttccatag caatcgtcgt
     3721 caacagtgta ttgataattc cttggaaaaa tcctgctaac acaatgctga tcggtacaag
     3781 gactggcata gaagttccag ctcctaataa cagcaaacaa ataatgaata agacaaatgc
     3841 tgctaaaatt gtccgataag tcgtaaactt ccgctcaaga gccggtgcaa cgaaaatcga
     3901 agcaatagcc agaagcacac cccagccaaa aaatacaaaa ccgacttcta attcagaata
     3961 gccaatcaat aaaaatggcg catacgcaag taaagtaaag aatccgaaat tatataacaa
     4021 tgcgattagt ccaactgaac gaagtttatg attgcttaac gctttgacac cttcccaaag
     4081 gcctactttt ctctttggtt tctcagtttc aggtaaaagt accgctaggg ctataaaacc
     4141 gcaaaacatc aaaaatgcta ccccaaaaaa tggataacgc catgaaccac tgcccaagac
     4201 cccaccgact agagggccaa cagccattcc gcagccaagt gctgcttcat acatcatgat
     4261 tgctttttct gtgttaccaa ctaaaatact gacgatcgtt gctaaagcag tagagacaaa
     4321 taaagcattc ccgacacccc agccggcacg taacccaatc agcattccga tattcccaga
     4381 taatccgcca agaccagcga atacaacgat gataaacaat cctgtcatca atgttttttt
     4441 caaaccgatc cgactagata agaaacctgt aaatagcatc accacaccag tgactaacat
     4501 gtaactagta aataacaggg tcgtttcagc tggcgtagcg tttaactgtg cagcaatcgt
     4561 ttttaagatt gggtctacta gacccactcc cataaaagcg atcatacagg tagctgctac
     4621 aactagcgct gaacttttcc cctttgtttc ttcatttgtt tgttcttgta tatctatttg
     4681 attctccaat atttccatcc ctttctctct attagcatca tacatgattt attttacatg
     4741 taaatatgca catgtaaaac gtggaaactt tactgtgata taataaagaa aataagacga
     4801 gaggtaggat ttttatgaat actttagcct atatattatt atgcgtgctt ttatccaaac
     4861 cacgttctgg gtatgaattg aagcagctga tcacaatttt ttgggaagcc catcacagtc
     4921 agatctatac aacgcttgcc aagttagacc agcagggtta tgtgaccgtt ttggacaaag
     4981 acgagcactc tcaaaaaaag atctaccact tgactgatga aggacgtgct cttgtagagg
     5041 aatggatcaa agaggaaact cccgctccta cgcaaaaaga tgaatttcta gcaaaaatct
     5101 atgctttttc gacgctcgat aaaacaacag ctactggact gctattcact agagaacaac
     5161 agctgaacaa aatcatgcaa aaaaatcaaa aaaaattgaa tgacctctcg ctagacaaga
     5221 aacaagaatt tggacgttat gttgtgatcc agcgcagaat tatgctttgt cagcaggaac
     5281 tccaatggtg tcagcaagta agagaacaaa tgcagcagtt ttttgagtaa gggataaaca
     5341 gaacctttct agaaatatct attgaagagc aaattatctt caataaaaga gcaatacagt
     5401 tggaaaagaa aaagcgatcc gaacgacagg ccaaaaccta tcgttcggat cactattttt
     5461 ctagaactgc attcaagaga ctatgacaaa aagcatgcca aggccttttt tcaaataaca
     5521 gtgttccatc attcattact ttgaattgct tttagcacac ttcttgaaag cgggccaacg
     5581 atgatcaact gcaacggtaa agcggcaatc acattcattt tccaagcatt catataagca
     5641 gagaataatt gatcaggtat tccttgttcc atgatcacac cgaataaaga catacatgtc
     5701 accattccta aaaccatcaa gcatgaaata gttaagatca tttggacctt gctttctgga
     5761 tttactggta gtttaaaggc aatttttttc gccagcacac cgatgatgaa gacatccaaa
     5821 atgaaagcca ccacgaatcc aggcactaga ccaatcaaca aacttgataa atgaagatct
     5881 tgatgcagcc ataaattata cgcgctcatc cccaagacca tcaaaaaaca catcattgtt
     5941 gtaaaaatta ttccttcttt tttatttgta ggcaatctgt accactcctt taaatttgtc
     6001 acgaaaatca tcgtagcaca tttaaaaatt tctcgtaagt acttttttta ttttctgccc
     6061 ttttatttca taaacttcat cagccgtacg ctcgacaaag taagaatcat gagaagtaaa
     6121 caaaatcgtt cccggatata tttgcattag cttctctaaa gcttcgatcg ttgccatatc
     6181 aataaaatta gtcggttcat ctaacagcaa cacattactt ggcttagtaa aaagcaaagc
     6241 aatcgtcaga cgggtcgctt ctcccccact taatgtacat aacggtttcg tcaaagtttc
     6301 ctgagcaaac cctaagtgat tcagcaaact acggacaagc gattctgaaa actctgtttg
     6361 tctcatgaca aaacgaatga tggattcttc agatgtcatt tgataaccct tttgctgata
     6421 tacttgaaag cttactttcg gagagagaag gattccttct ccttgtttgc ggatatgttc
     6481 taataaggtt gtcttacctg aaccattttc accgacaatc gcgatttttt tacctaaaga
     6541 aaactgaaaa tcaccattta ccagtaatgt tctcccactt ctttccaatt ggacattttg
     6601 tcccatgatg ggatagcggc tgtggagttc aagagatttt ggtactggaa aacggatttg
     6661 tttcatctgc tgcggcttgg ttgtttctcc tattttttgg agtcttctct ctaatgtttt
     6721 cgcattcttt tggatggctt tttgtacact gtctttttgt ttagaggaag acaaccgatc
     6781 cggtctgatt tgttgctttt tctttttact ggacactttt tccaactttt ccgcttgttt
     6841 tttcttttct tggatcgatt tttccaatcg ttttttctct ttcatgatcg actctgcttc
     6901 tcgtagctga gtctttttct ccaattcctt ttgagtgaga taggcactat aattccccga
     6961 aaactctcgg atttctccgt cttttacttc ccagattttc gatgctagct catctagaaa
     7021 atagcgatca tgactaacaa aaaccagcgt accataataa taacgaagct cttcaatcag
     7081 tcgatcaata ctttttctat ctagatgggt tgttggctcg tctagaagca tcccttgttc
     7141 ataagaagaa agaagacgta tcaactcgat ctttcgttct tcccctccgc tccatttccg
     7201 ctctgaagtt tcttccagtc ttaactggaa atgactggct aattccaaat ttaggtcctt
     7261 tgcttcagcg agataggtta attgaggcag gtagttgaag gtgatctctg tttggatttt
     7321 cccatgatcg ggaaaaagtt caccggctat tagtttgagc aaagtcgatt taccgacacc
     7381 attttctcct atgatcccta tacgatcttg ctgatagaca cgaagagaat caatagatag
     7441 ttctagctgg ttgccaaagc tgacttgtag atttgttata tttactgcta aattttccat
     7501 atgttttaca ctcctatttc atgtaaaaca acaattatcg atactaaaaa agagcagaaa
     7561 aatctgtttg caaaaaaact gcatcacatc ttttcctact ctgaacgaaa taactatgac
     7621 taaatcacca aatctgtcat agctttcacc tattctactg tcgttcgaaa aaatggacat
     7681 actccatcca tgaaattgtt gtttatacat gtcttttgaa tttatttagt tgttattcaa
     7741 aagttcgaaa cgtaatttca tcgatgcagt catttttccg acctcctttt ttattaggtt
     7801 accacacacg attcaaaaaa agcaatagct actgaatagt aactgaaaac cttttattcg
     7861 aacgtagtga tcttttctgg tgctaaacga acagactcgt aaccttcttc taccgctttt
     7921 cctattgata aaataataac tggaacataa cggtctttat ccaaaccaaa tgcctctgcc
     7981 aactgatctg cttcgactcc accaattgga tttgtttcat agccatgatc acgcgcaact
     8041 aacatgaatt gcatagccgc taagctagaa tcgactttga ctacatcgtt catttcttct
     8101 cttgaaaaac tcttgtaata aggaatgatc gcagctaatt gttgatctct gacttctttt
     8161 ggcatcttcc ctgattcgta agcttggtta tagatttctt cgccatattc gtaacattgc
     8221 atatcgccaa aaatcatgac cattgcagaa gaagtatcat tttgtctcgt attgaaacgg
     8281 atcaaaggac gcaagatttc ttttgcttcc tcgctttcag cgaccacaaa gcgccatggt
     8341 tgcatattga cagaagaagg tgcagtcgtc gcttctttga tcatttccag catttcttcg
     8401 tgtgggattt tgtatgtttc atcatatacg cgaacggatc ttctaccagt cataattgtt
     8461 gaaaaatcat ttttttgttc cattattatt aacctctttc gtttctgttg ttatattgca
     8521 aagttgggta ttcaatttat ctaaaagtgt tagaaattgt ttcatttcta catcgctgaa
     8581 agccggggat agcacggaac ctgtatcttt atggtccatt tcacaatgct cgattttctt
     8641 ctgggctttt tctgttagcg ctacatacac ttcccggttg ttctgaggat ttctctgacg
     8701 cgaaacatag ccatttgctt ccaatacctt cagatgacga gtaactgctc cttgatcaat
     8761 ctgcaacttt tcttgcaaac tgctttgtag caatggattt tcttctttca gtctcatcat
     8821 gatctcgtat ctagtaagac ttataccgat attttcttca aagatctgga cgattttttg
     8881 atcggctaat ttgatttgat ataaaaggtg actaatctct tctaaagaca taatttcctc
     8941 ctttctaata attgactcgt caacgattga cttgtcaata atagaataaa aaaagaaaaa
     9001 agtcaaagaa tcagctgtgt aataaaaaaa acgctctcaa tagaaaagta tagagaaaag
     9061 aaattttttg tagaatgaac ggaaaaaatg ataaagaaag cacaacgcta aaaccaagat
     9121 tattgattct tatcaaacta tagaagatag ctcttctgca gtagaaaatt ttacaccgcc
     9181 aaaaatttcc gaaatcgcct tttgcaagaa tcccgtcgaa tattagaaat tggatcagta
     9241 tgcttaaaca gtaataaaaa agttatgaca agatgttgtc acaacctttt ttgagtaaac
     9301 ggtgttcaaa aacttgcatc tgcggtaata agttcaacca aacaaaaaac atgaaaatct
     9361 gtttttgttt ggctgttctt actacttgat gcagggcagc tttttcacta cctcttttta
     9421 ataaccataa aatcctattc cttcatatct ccagcccatc ggtgttagcc aatctctttc
     9481 cttagcactc aaggtatagt gatgggtacc agttttttca tttggattaa atagtcgata
     9541 aattggcttg gaaccaccag aataaaaact tagaccttca taacgccaac cgatttttgt
     9601 taaagagttg atctcagatt gtgccattgt gtaatgatgg tctccactgt aaggattgta
     9661 taaacggtag acaggttgtc cgctgtttgg tgcttgccaa gcaatccctt catattccca
     9721 gcctacagtt ggcagataat ttttttctcc ggcattcaat gtatataaat gttctcctgt
     9781 atgacggtta tacagacgat acatcggaga agttgaagtt ggtgctgtat catactgtgt
     9841 cagattatag gtttcgatta cattgttcaa tgtagaacca taattaggcg cagtggcata
     9901 acgacctgtt agccaagccg tagcatcttt gtaagagttc gtattgcttt tccatgcacc
     9961 ggaatagtag taaacgcctg gctgaaaaga agtcgtcttc aataccgtta cgttatcttg
    10021 caatgattct ttgtacgaag gatattttct aaaagcggca ttcaccgtaa tccattgccc
    10081 attcacatat tccgaagtag gcatcgtaac agattggcca ttatagcttc ctttaatccc
    10141 aaatagattg taattagggg cagaagccaa agtactgttc ccccagccac tttcaacgat
    10201 ggcttgagca atcatgactg atgcatataa gtcattggca cttgccaaac tttgtgcagt
    10261 tgaggcaata ctatcaatga acgcttgttg agaattagca gatgagcgag cataaaaagc
    10321 atagccagta tagttttcga attcagacaa atctgcttga tgggtatgtg tcaatgtttg
    10381 cccatctgtc aattggttcg tgatttcttc tgcattctct ggattgttct gagcttctgc
    10441 tgatgtactg tcttcggttt gtgtttgttc tgttgtttca gttgttggtt cttcggttga
    10501 aattgtattt tctgagacag tagctgactg caaagtactc tctgtatcgt tttcaattcc
    10561 tgtttctgaa gaactcgatg tctgagttgc gacagtttct gtcgattctt cagctagcac
    10621 tgttgtgttg gcaactgcta gatttcctaa aagaataacg ctaaataata aattctttgc
    10681 tttcatcttt tttctccttt gaattatgaa acttttccta aataacagtg taactaaaaa
    10741 agtaaaagca agtcaatgtc catatttaaa taacatttgt ttctttttga taaatttgct
    10801 ttttctacat aaaaagacgt aaatgaagaa tattcttctc cattcacgcc ttaaattgat
    10861 ggtcgtttga ataactattt aacaagtaat tctctttgga tattatccgt tatggattat
    10921 acttcctgtg tcgtcggacg gataatcatg ttactgacag acatccggtc tggcgtttct
    10981 atcgcaaatg ctacagcaga agctatatct tcggaggtca atccccattc tttttgcgct
    11041 tcatgaagtt caagagcagc ctttttgtcg ctgatggttt gatataattc agtttgaaca
    11101 gctcctggag agatgatcgt agatttgatg ttattttgcc gttgctcttg acgtaatcct
    11161 tccatgattg cacggacagc aaattttgtt ccgcaataaa cggcggaatc aggataaaca
    11221 acatgtcccg ccacagaatc agttgaaatg atatgcccag atttttgttt ttccatgatt
    11281 ggaagtacgg cggaaattcc atttaacaca cccatgatgt tgatatcaag catattttgc
    11341 cattcatcac ggtgaccttc aactaatgga gcggttggca tgattcctgc attattgtac
    11401 aatacatcga ttcgaccgaa cttttccatc gctaaatcga taactgcttg gacttgtgca
    11461 aaatctctta catctgctgt ttgaatatat agctcagctt ctggtaaaga ttcttttatt
    11521 gcttttaggc gatcttctcg gcgtgcagct ataactagtt tagccccttt ttctgctaat
    11581 agcttcgttg ttgcttcacc gattccgctt gaagcgccca tgatgacaat cactttttct
    11641 gataaatcat tcattctatt ctcctcctgt tttgaaaaat ctcttgctta tactcactat
    11701 ctcttatttc atcttactgt tcaaataatc cgttcgacag gtttgacgat tttggcaggg
    11761 attcctgcaa cgatggtatt gtctgggaca tctttcgtaa cagttgcatt cgctgcaaca
    11821 atggcattct cgccaatcgt tacgcctggc agaatagttg ctcctgctcc gatccaagca
    11881 tttttcttga tgtggattgg ctttactaac aagcctcttc gttcttttgg atcggttaga
    11941 tggttcaccg tgatgaggtt gacacgagga ccaatcaata ctgaatcttc taaacagatg
    12001 ccacctaagt caacgaacat cacttgacga ttgataaaga catttttccc tatactaata
    12061 tgtttgccga aatctgtata aaaaggcagc agaacagtca ccgtatcatc tactttgtca
    12121 ctaatgatat tttctagccg tttgcggata tctgctggtt catggtaccc agtattcagc
    12181 gctgctaaag gctcttcgtt ttcagcttgg atctgatgga tcacttcaaa gagcggatcc
    12241 gttactcgaa tctctttatt gacgatttgt ttttcgatga ggcttgtcat tttgctccct
    12301 ccttttatcc ttactttata acggacggct tatcatgtct aatacttata aaacatactt
    12361 aactattcct tttaactata cttagtggta tgctttttta aaaacaggag ggaaaaaatg
    12421 gatattcgta tactgacgta ttttttagca gtagctagag aaaaaaatat cagccgagca
    12481 gcaaaatctc tgcatatcac gcaacctact ctctcaaaac aactgaaaga tttagaagaa
    12541 gaacttggtg tgacactttt cactcggggc tcccgagaga tacagttgac ggattctggt
    12601 cgatatcttt attcaaaagg aaaagaaatc ttgtcgttgg tagaaaaaac ggcgaacaat
    12661 ttaacaaatg acgcaatcat ctctggagaa atctacattg gcggaggcga aactaaagca
    12721 atctctttca tttctgatag ccttcattcg ttgatcaagg cgcatcctga tatccagttt
    12781 catttatata gcgggaatgc agatgatatc atagacaaac tggatagcgg attacttgat
    12841 tttggactcg taatcgaacc gacaaacaaa caaaaatacc agtcatttcg actgccagcc
    12901 caagatcgtt ggggactttt gatacatcga aatcatcctt tagcaaagca aaccgtcatc
    12961 catccgcagg aactgaaaac tgttcctttg atcatttccc ggcaaacgtc agtggacagt
    13021 caactagctg aatggctagg tttcagcata gatcagttat cgatcgtcgg aacatataac
    13081 ttattgtaca atgcttcttt gatggtcgat aacgggactt ttggtgcatt agctctagat
    13141 gggatcatca atacttatgg gacggaaaaa atattcatcc cactcgatcc gccattacaa
    13201 gcttcactga atctcatttg gaaaaaagac cagcctcttt ctcctgctgc tcgtaaattt
    13261 ttagatgtcc taacggagaa atacagttaa gtagagtttt tttccttcgc tttcagcttt
    13321 ttaacaacga ataagataag caaaagatcc aacgattaaa taacataagt ttcttaattt
    13381 atttcttcct tttatttctg ataattccag taaattacta taa
//
LOCUS       DANKKG010000067        13384 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000067 DANKKG010000000
VERSION     DANKKG010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13384
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.067"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(54..596)
                     /locus_tag="PW004_001660"
     CDS             complement(54..596)
                     /locus_tag="PW004_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HDL2726633.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            731..1120
                     /locus_tag="PW004_001661"
     CDS             731..1120
                     /locus_tag="PW004_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726634.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1124..1888
                     /locus_tag="PW004_001662"
     CDS             1124..1888
                     /locus_tag="PW004_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL2726635.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            2017..2964
                     /locus_tag="PW004_001663"
     CDS             2017..2964
                     /locus_tag="PW004_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2726636.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3094..3834
                     /locus_tag="PW004_001664"
     CDS             3094..3834
                     /locus_tag="PW004_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726637.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(4125..4793)
                     /locus_tag="PW004_001665"
     CDS             complement(4125..4793)
                     /locus_tag="PW004_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL2726638.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            4927..5214
                     /locus_tag="PW004_001666"
     CDS             4927..5214
                     /locus_tag="PW004_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726639.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            5630..7168
                     /locus_tag="PW004_001667"
     CDS             5630..7168
                     /locus_tag="PW004_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HDL2726640.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            7165..8130
                     /locus_tag="PW004_001668"
     CDS             7165..8130
                     /locus_tag="PW004_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2726641.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            8117..9439
                     /locus_tag="PW004_001669"
     CDS             8117..9439
                     /locus_tag="PW004_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL2726642.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      9502..9606
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            9680..10423
                     /locus_tag="PW004_001670"
     CDS             9680..10423
                     /locus_tag="PW004_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2726643.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            10438..11430
                     /locus_tag="PW004_001671"
     CDS             10438..11430
                     /locus_tag="PW004_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2726644.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            11442..12179
                     /locus_tag="PW004_001672"
     CDS             11442..12179
                     /locus_tag="PW004_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2726645.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            12169..12891
                     /locus_tag="PW004_001673"
     CDS             12169..12891
                     /locus_tag="PW004_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HDL2726646.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(12975..>13384)
                     /locus_tag="PW004_001674"
     CDS             complement(12975..>13384)
                     /locus_tag="PW004_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HDL2726647.1"
                     /translation="QLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEKQAAP
                     VAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYLNGDY
                     SAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         4406 a         2291 c         2783 g         3904 t
ORIGIN      
        1 tgtctaacat ttaaaattta atttagatat tagacagttt ttaagtttgt gagttatttt
       61 ccttgtcgtt ttctcacttt tttagtccag aaaccacctg aaatatattt tggctcataa
      121 gaaatgataa aagcacgctc ttcgatttct ttgatcaggc gatacagttc acgttcgctt
      181 tttctaggag aaagtatttc gagaactagc cgctcacctt ccagccccat tgcagaagtc
      241 tgtgtcacac cataaccgtg ttctctcagt atacccggca aacttttatc ttctgcgcta
      301 tttgctggca aaatcgttgt gaccatgatg tatcctaatg ccaatttgtc ttcaatcaaa
      361 attccgacac tgatacctac tgcgtaacct aacgcataga caaataaatt cagtggatta
      421 tctaaacgat ctaaaacaag ggataagccg acgatataaa tcgtgatctc gaccatactg
      481 acaagtggag caatcagtcg atagcctttc attgtcaaca tgaaacgtaa cgtgttcaga
      541 gtgatataag caaaattaat gaggaagatc atccccaaca tttttggatc aatcatttgt
      601 aaaactcctt tcatttatat aacaacaata tcaaaaaaaa tcgtaagtga agaaaaattg
      661 aaactttata catacaaaaa aatgataaaa tgattataat agaattataa agataggtta
      721 ggtgattaaa atgaaaacgg tcaatcgtgt gttttgtgcc agccacgtgc taagtctcct
      781 tctcagtata tacttaggtg tctattcgat tcaacagcca attgaggtag agaacattgt
      841 ttttcagatg tcgctgacag atggtttaat tttaagcgtt tttttctttt tgatactgtt
      901 tattggtaat attttcggta gcgttgccag ttttttgaat ttttctattt atccactcct
      961 ttcgctagca ttaggagtgg tgggactttg ttctctgtgc ttatttcccg agccattcag
     1021 tcctgcattt atattatttg gtacactgaa cctgttccag acaactgttg gagcatggtt
     1081 gctatggcga tcaggaaatt taatgaaaat aggtgagtaa caattgaatc caattatatt
     1141 gaaacgttct gtccatcata ttccagtgtt agaagttgtt ctgaaagaat taagagagga
     1201 acgtattccc gttgtcatct attatcacgg atggcagaca agtaaggagt tagttttgac
     1261 gcaaggaaga aaaattgcgc agcagggcat tcgtgtgatc ctgccagatg cgatgaatca
     1321 tggggagcgc aaacagccgg tttcttctat tccatcctat acattttggg atagtatcta
     1381 cggaaatctg tttgaatttg acacacttat caagcattta gaaaaaagag gtcttctaca
     1441 tgatcagctt gcagttggcg gggtttcgat ggggggaatg acaacatgcg ccttgttggc
     1501 taaacatcct catatagtag cgggcgtgtg tttgatgggt tcccctgcac ctttggaata
     1561 tggaaaagaa atcgtaaggc gcgcatcgga atataattat gtcgtaccaa aagactattt
     1621 tgatttgatt tcatgggtta agagctatga tttgtcattg cagccggaaa agttagctgg
     1681 acgtcctctg ttcttttggc atggacaagc tgacgaaaaa atcccttata tacaaacgga
     1741 acgatttgta gaaaaaaatc ggcataagtc ttacggaaaa gaaattgttt ttcgttcctc
     1801 aaaaacacag cgacatatgg tacaaatacc actaatggaa gaagcttcca cttttttagc
     1861 aaaaaatttg ataaaaaagt ataaataatt gaaacggaaa atcactcatg acgatagagg
     1921 attttccgtt ttttattttg ggctataata aactctgtta ggaaaatttg gtataattga
     1981 tagtaagaat gacaacatgt tagaaaggaa gagagcatgc ccactttagc agatgttgca
     2041 aaacgtgcca atgtttcaaa aatgacggtc tctcgagtca tcaatcatcc ggaacaggtg
     2101 actgacgaat tgaaagagtt agttttctca gcaatggctg aactggatta ccgtccaaat
     2161 attgctgcaa aagcacttgt cacgaatcgg tcgcagatca tcaaattatt tatactggaa
     2221 gaaatcgaca caactgagcc ctattacatg aatttgctga tgggaatcgc taaacggatc
     2281 ggtaaagcgc attattctct ccaattagtt acaaatgatg ccttcgacgt cggttcttgt
     2341 gatggataca tcatcacagg tgtcagaaaa aaagacttcg attggatcga ccgtttggaa
     2401 aaaccagttg tcttgtttgg ggaaaattct gaaggatatg attatgtaga cagcgataat
     2461 gaatatggta cagcaaaagc aacgcaatat gccttagaaa gagattatca aacgattatt
     2521 tatatcggta tcgaagtgga cgagcctttt gaaatttcaa gggaaactgg ttatttatct
     2581 gtgatgaagg aagttgatct tcctagtaaa atcttgcggt ttccaaatca ttcgacagaa
     2641 gtagaaagat atatgacaga aaactgggca gattttacag ataaaacttg ttttgtatgt
     2701 agttcagatc ggctagcact aggagcagta agagggatta taaatgctgg aggacacttg
     2761 cccgaagaat ttggtgtgat tggatttgat ggggtatttt tagaccaaat cagttcacct
     2821 aaattgacaa cgatcaagca agatattata cgtatgggtg aagtatgtgg agaaatgttg
     2881 ttaaaaaaaa tagaagaaaa aggggcgagt caaggttatc gtcgtttttt tcctgaacta
     2941 gttatcagag agacaacgaa ttaagtctta ttactatata tatatttatg aaattcatcg
     3001 ctattttctt aaaataatca gcgcattgct actgaaaaca atgaatctgt gctattattg
     3061 agaaaagttc catttttgag aaggatggtt tgaatgaaag aaatcagacg aaaacatttc
     3121 cgttttcctg cgtacgatga tgagttaggc gtaaaattaa ccaacgacaa gaagcgagta
     3181 ttatttgacg gacaagaaga tcttttgact gaacagaatc agtccccgtt tgaacagatg
     3241 aaagaatttc atttcacgaa cacgaacacc gagaaaatcc gtcgcaagcg tcgtgcaaca
     3301 aatggattga agtcagctcc gcgtaaggaa aatttggaaa agcataaagg aaaattaccg
     3361 gattataccg gccattacca atcagaaatc acatcgacag gacgaaaaaa actttttggt
     3421 gattcctctc gttctgtcag acaaagtgaa acaaggacag agtccaagca tactacacga
     3481 aacgcatctg ttgacacgaa ggtgcgggag cgttcttatt ttgttcctaa atatgtaccc
     3541 gcatcgatta tcccagaccc tagagaaagc aaaatttctg aggatcattt aatcgaatcg
     3601 atgaaaaaaa cggatgactc ttatctgttg ttcgatacag aaccgacgcc gtatcaagaa
     3661 aaaagaaatg gtgagccgtc tgtacgaaca ttcaatcgaa caaaaacgtt agaggaaaca
     3721 aagcagtcag aagaaaaaaa atcgaaaaaa agccatggta tcttagaacg ctcattacaa
     3781 ggattgatcg atgatcaagg aaactctttg ggagaaaata gctattttaa ataagtgata
     3841 tacaaattta tattcggcta cagttggaaa ggaaacgctg cggcgtttcc ttattttata
     3901 ctctatccga aagtttatat cgtatcctca aatttttttg agtttaagcg aattttttct
     3961 cccaataagt aagaaaataa tggcaaaata aaaaaacaaa ctattttcgc agatcgtttg
     4021 caaaaatagt ttattttttt ataaagaaga ttttactgaa taatatcatt ttagtttaca
     4081 taatgatatt tctgtaaatt aaataaagta gtgaaaaata agaatcagtg ctctaactgt
     4141 tcaagaggat caagtggttc aaatgtttct gtagtctgtt ttgcttccaa ttggggcaaa
     4201 acagatccaa tttccagata cttcacttta ttcaatgtat aagatagaat agaacagcta
     4261 agtaatgtat acagaaaatg tgtgagatcc aaataaagca aggagacaac taaaatacca
     4321 aaagtaatca agaaaagact tttgccaatc ttaatatgaa aatatttttc aatggcttga
     4381 taaatgatat ctgttccgcc tgtgcttccg ccgaatcgaa agacgagacc gcaaccaatg
     4441 ccagaaagaa tacctgtcat caaactattg atcagtagac tatctacagc aaaatgtata
     4501 tgaaggctct cccataaatg taaaaagaaa actagcgcaa atgttccgac gatcgtccgg
     4561 agaaaagcgt gttttccaaa aagaaacaat gagataagca ataaaggtaa attaaaaagc
     4621 agtgaagtga cagaaggagc gaatccaaaa agattatgca aaagcaaagc aataccagta
     4681 accccaccat ctgccagctt agatggaata ttgattcctg tcacagctaa tccgtagatt
     4741 cctgcgccta aaatgattcc gcacaattct gtaaataaat ttaatttttt catttctagc
     4801 ctcttttctt ttgaaaattt tcagcaactt ctttattata ctgacttata ctcttacttc
     4861 aagaggaaaa tgaaaaaaaa ttatttttgg ctgtttcttt ttatttatca aataaggagt
     4921 ggaaaaatgc gagcagcaga cttattgaca attcttacaa aatcacccaa aaaaacagag
     4981 tatcaaggag tttttttggc tcaagaacaa aaactggtgc ctgtgacaat ggtccagata
     5041 aataaagcaa atcaatttat ctttttattt gaacacggca aagcaccact tgctatgaaa
     5101 gacatctttc tttttttgat gaagaaccgt gagaaagctg tttattatca gtcaaaagaa
     5161 gaactcattc ctctttatgg agtgaaagag atagaaaata aagtggtgat ataagtaaaa
     5221 ataagaaaac aagacgttta tgatgtcaaa acaaccattt tttacatttt tggcaaaaaa
     5281 gtgtcatgat acagtataat attcgttgaa caggccaata aaggtgttct cccccacgag
     5341 atttctactt tattggctgt ttctttttat taaaataaaa gttgtgtgtg taaaaaagat
     5401 atagctgatt ccaagtgaca atttcgtata gatttttatg gtattcgtag tgaaaaaacg
     5461 agaaccaaaa agttgatccg actttgttgc ttggattctt tttttgtcag catatttaga
     5521 ggtatttttt tctttttttt gttcgtgctg taaggttaag gcaagacaag ttagttggga
     5581 aaaaggaaag aaatgtttat acttaaagaa taacagaaag gaaggtcgca tgaataattt
     5641 tatacaaaca ttaagtgaaa gaaaaggcga gttgctggaa gctgcatttc aacatctgtc
     5701 catttctctc atttctttgc tgattgcagc actgattgct attcctttag cgatttgggc
     5761 agccaatcat aaaaaaatgg cagaagtctt attgcagatc acgggtgtgc tccaaacgat
     5821 tccttcactt gccttattag gtttacttat cccatttgta gggatcggaa ctgttccagc
     5881 attgattgca cttgttattt acgctttgct tccgattttc caaaacacgt atattggtct
     5941 agctgaaatt gacccgtcta ttgaagaagc agcggtagca tttggtatgt ccagaatgag
     6001 acgcttgctg aaagtagagc ttcccatcgc attacctgtc attatctcag gtatccgaac
     6061 tgccttagtt ttaatcatcg gtacggctac tttggcagca ttgatcggag caggtggatt
     6121 agggacattt attttgttag gtattgaccg aaatacacct gttttgacat tgattggtgc
     6181 gatcagttct gccttgcttg caattatatt cagcggactg attcgctggt tgcagcataa
     6241 gaaacctcga tattccatca ttactttagc agcagtggtg tgtttatttg gcggcgtttc
     6301 tttatatcag tctgccttgt ttaaaaatga aaccatcacg attgctggaa aattaggagc
     6361 ggaacctgaa atcctgatcc aaatgtataa agaattgatt gaagacaaca caaaaacaga
     6421 agttgaatta aaaccgaatt ttggcaagac gagttttttg ttcaatgcac tggatcaagg
     6481 tcaaatcgat atctatcctg agttcacagg tactgtccta gaaagcttgg tgaaggtacc
     6541 tgaagcaatg aaaagtgctg atttgacaaa agaaaaaaca tatgacgaag cagaagaatt
     6601 attgaataag caattcgata tgacattatt aaaaccaatg gcttaccaaa atacgtatgc
     6661 cttggcagtc aagagatctt ttgcgaacga acatcaactt aaaaagattt ccgatttggc
     6721 agctatctct gatcagatca aagcgggatt cacactggaa tttattgatc gttcagatgg
     6781 atataaagga atccaagaag catatggctt gaatttttca agtgtacaaa gcatggagcc
     6841 aagtttgcgt taccaagcaa tcaataatgg agatgtcaat attgtggatg cctattccac
     6901 agatagccag ctcaaggagt acgatctagt gactctagaa gatgatcgag ggttattccc
     6961 agcttatcaa ggcgcaccgc tgatgaaaaa agattttgct aaagaacatc ctgaaatcgt
     7021 taaagcactg aataaattaa gcggaaaaat aacagaggat caaatgattg atatgaatta
     7081 ccaagtcaat gtcgaaaaga aaaagccgac agaagtagca cgaatcttcc tgaaaaatga
     7141 aggattagta aaggaggaag atcagtgact aaattaatcg agttcaaaca tgtgcaaaaa
     7201 aagtatgatg gaaagtatgt catcgatgac ctaaatctgg caatcaaaaa aggagaaatc
     7261 tttgttttgg ttggtccttc cggtagtgga aaaacaacta ctttgaaaat gatcaatgga
     7321 ttatctaaac catcagcagg agacatctac ttcaaaggaa aatcgttaaa tgaatataat
     7381 ctgcagaaaa tgcggtggaa tatgggatat gtgctgcagc aaatcgcttt gttcccgacc
     7441 atgacagtca agcaaaatat tgaagtcatc cctgaaatgt taggttggga aaagcagaaa
     7501 agagcagacc gtgtagatga acttcttcaa aaagttggct tatctcctga tatttatcga
     7561 gatcgtatgc ctagagagtt gtctggagga gaacagcaac ggatcgggat catccgagct
     7621 attgctgcaa gcccagatgt gattttgatg gacgagccat ttagcgcatt agatccgatt
     7681 tcaagaaact cattacaaga attggttttg tccttacacg aagaattggg cacgaccatc
     7741 gttttcgtta cgcacaatat ggaagaagca atcaagctag gtgatcgtat tgcatttatg
     7801 aaggatggag aaattatcca atgtgacaca ccagagcagt tattgatgaa tcctaaaaac
     7861 gactacgttc ggcatttctt tgatgaaccg aaacagacga aagagtggcg agtagaagat
     7921 ttagtcgtca atggatattt cttaaatgaa ataccagaaa atgctcgtca acaagtttgc
     7981 tttgatacgc ctatgaaaga agtatattcg cttctttcgg tgtatccaag cattgtgata
     8041 gtagaaaagc aaagaagtat tgggtcgctg acaagtaaag aaatctttgc ttttttaagt
     8101 cgagaggagg aaggaaatga aaacatatga tgcaatcatc atcggttcag gtgtgagcgg
     8161 cttaagtgct gcttatggat tgaaagaagc aggtaaaaca gtactagtgg tcgaagaaga
     8221 tctatggggc gggacttgtc ctaaccgtgg atgtgatcct aaaaaagtct tgttaagtgc
     8281 agtagaggca cgtaatcggg taaaacaatt gagtggaaaa ggttttaacg agatcccaac
     8341 tgccaactgg gaagaacttc aaaaattcaa acgaacattt acggatccag tcccagaaag
     8401 ccgaaaaaaa caattagcag aagctgaaat cgatcatctt tcaggaaccg ctcgtttttt
     8461 ggatgattca tctattgaag tcaacgaaga agtcttccat gcggattact tagttttggc
     8521 aaccgggcaa agacctacga tcttaccagt ggagggaaag gaatacttga aaactagtgc
     8581 agacttttta tcattgcctg tactgcctaa agaaatcatt tttatcggtg gtggttatat
     8641 tgcttttgag ctagctacga ttgccaatgc tgcagggagc aaagtaacca ttgtccatca
     8701 taatcaacgg ccattaaaag aatttgaggc gtctttggta gaggaagccg tccatcaaat
     8761 ggaagcatct ggcattcagt ttgcttttgg tgttgaaacc caaaagatca tttcagaagg
     8821 tactcgctat cgtttagtcg gaaaagagac ggaattagta gcagatatga ttttttgtgc
     8881 gactgggaga cagccgaata cagaatcttt agcacttgag caagcgaata tcgtctttga
     8941 taaacacgga attgcagtca atgattatct gcaaacgagc aatccaaaaa tttttgcgtg
     9001 tggagatatt gtttcaagga aaaccccaaa attgacacca gtcgccactt ttgaaggaaa
     9061 ttatgtagct aagcggataa cggatgcaac aagcgaacct atcaaatatc cgattatacc
     9121 tacgatcgtc tacgccagtc ctaaacttgc tgaagtagga gttacaaaaa gtcacgcctc
     9181 ttcgtcagat caagtggtag agatggatct taccagttgg ttcacctatc atcgtgtcaa
     9241 tgaaccagtt gctaaggctg aattgacctt tgaccagcaa aattatttga ttggtgctgc
     9301 agtgattagc gagcaagcag acgaattgat cgatgatttg acactcgtca tcaatcaaaa
     9361 attgactaaa aaagagttgg atagctatat catggggtat ccgacattgg caagtgatct
     9421 ctcctattta ttgaaataat cagcatgata actaaagcgc ttgttgcttt ttccttcttg
     9481 tgctacactt taaaaaatga atacctgttt ctggggatgt cgtagacgac atgagagtaa
     9541 gttgtgaact tagacccatt gaacctgtca gttaatactg gtgaagggag aaacatagca
     9601 gacgcttgaa gcgggttact atgtcgtgcc catttcaagc gttttttgtt attcatatta
     9661 gaagaaagga ggtaacaaaa tgaagaaaaa tctttatcca gcagtcagta tggcattcat
     9721 tttaattatt tggcagattg ttgtcatagt ttggaaaata cctgccttta ttttacccgg
     9781 acctgctgct gtattttctg cattagcggc agatgcacaa gtattacttt cccattctgt
     9841 cacgacatta aatgaagcag tgcttggttt ggtaatagct agcgttcttt cttttattac
     9901 tgccttggca atggattatt ggaagttttt agaaatgtct ttttatccgt tactagtgat
     9961 ttcacaaatg ttgccaatca tggtgttagg gccattactt accctatggt ttggtttcgg
    10021 tatgcttcca aaagtgatcc ttgttgtctt gataagctat tttccgatcg tagtttcttt
    10081 ttcggataca ttgaggaaag tatcaaaaga gcaaatcatc tttttgaaaa cgatgggggc
    10141 agatacaggg aagatttatc gaatatataa aataccagcg ggtttatccg gcttcttttc
    10201 cggtttaaaa gtggctgcta cttattgtgt cagcggagca gtcgttggag aatggctaag
    10261 cgctcaagca ggcttagggt attacatgat acgcgtaaaa aacagctacc agatcgataa
    10321 agtttttgct gcgatcattt gtgtcattct attgagttta ttgctgaatg gcgcttgttc
    10381 attattgaaa aaagggtatt atcgtatgct ttatattttt taagagaagg ggatcaaatg
    10441 aaaaaattat ggcttttatt accgctactg ttgcttttat ccgcttgtgg cactgcaaaa
    10501 gaaacaagta gcaaacaaga aataaaggat ttgaaaaaag tgacacttgt tttggattat
    10561 gtaccgaata ctaatcatac tggcatctac ctagcaaaag aaaaaggcta ttataaagag
    10621 gctggtttaa atgtccaaat catcgaacca ggagataatt cgacaagtat tggtctagta
    10681 ggtgcggaca aagcacaatt tggtgtaagt tatcaagaag atgtcactta tgctcacgca
    10741 gatggacaga atataccagt caaagcaatt gctacagtga tcaaacataa tacttctgga
    10801 ttcgccaccc tatcagacag taacatccat tcaccaaaag attttgaagg aaaaacatat
    10861 gcaggttggc aaagtccaag tgaagaagct gtcttaaaag ctgtgatgga aaaagacggc
    10921 ggggactttt caaaactgac aatggtagga agcaatggag aagggccaga aagcttagga
    10981 aaatcctcag atatccaatg gtactttgaa ggttgggata tgatcaaagc aaaagaagcg
    11041 ggtattgaac taaactatat cccattgaaa gaattagatg aacggttaga ttactatact
    11101 ccggtaatta ttacgaatga tcaactgatc aaaagtgatc cagaattggt acagtctttt
    11161 atggatgcca cgaaaaaagg ctatcaggaa gctattaagg atccaaatga cagtgctaaa
    11221 ctgttgcaaa aatatgcaaa agaaaatgat cggacgtttt tagaagaatc acaagcattc
    11281 ttgtcaaaaa attatacgga tgatcctgag aattggggat tgatggaaga aaaagtttgg
    11341 cgcaattata cagcttttat gcaggaaaac ggattgatca agcaagatgt accttcgcaa
    11401 gaactattca ctaatcagtt tataaaatag gagtgataga tatgtcaatc caaatcagcc
    11461 acttgaatgt agtcattgaa aacctgaaaa tattagaaga catccatttc actatcccta
    11521 aagatacctt tacatcgatt gtcgcgccaa gcggagccgg taagtcaaca ttgctgaaaa
    11581 ctttaacagg agtccagcct atgacttctg gaacgatcaa agtagacgat caaaaagtaa
    11641 ctggtctcca tacagcattt agttatatgc cgcaagaaga tatgttactt ccttggctga
    11701 gcgtctatca aaacgttacg ttatatcaaa aaatcaatca tctaacaatc aatgaagaac
    11761 aagtaaggga ttatctggat atctttgggt taaacggcta tgaacatttt ttgccggaac
    11821 aattatctgg cgggatgaag cagcgaacgg ccttgttacg aacaatcatg aatccttctt
    11881 cttacttgct tcttgatgaa ccatttggag cgttagatgc tatgaccaga gggcagatgc
    11941 aagactggat gttgaagctg cctaaaaaag caaagcggac tactttactt gtgacgcatg
    12001 atatcgaaga agcaatttat ttatcagaca ggattcttgt gctttccgcc cgtccggcac
    12061 atgtgattgc tgagattcaa gtgcctgaaa aacgacggtc acgtgaatgg ctactgcagc
    12121 aatcagagtt gaagcaaacg atttatcagt tattggcagg tgaattagat gttaagtgat
    12181 gctcattgtc atttagatgg gagtcagagt ttagcactcc ttcagcagga acacacgatt
    12241 ttgacgatca tcaattgtga ttcgccagaa gaatggaagg aaaacagaca gcttgcagca
    12301 agtaagactc aagccttaag ttacggtatc catccttgga aagcagatag ctatacgttt
    12361 gaacaagttg agcctttttt gaaaaaagca aggatcatcg gtgaaatagg gttagataat
    12421 atttggacga atgtaccgat gactacacaa aaaaaagtat ttgagcgaca gcttgctttt
    12481 gcagccataa atgagaaacc tgttgtactg cacacaaaag gatgcgaaaa agagatctta
    12541 aattatatcc aagcttaccc taatcactat ttgatccatt ggtattcttc tttggactat
    12601 caacaagact atattgattt aggctgttat ttcagcattg gtcttgattt gaaaaagaat
    12661 ccagcagtct ggcaactagc taaagcagtc ccaatcgacc acttacttat tgaaacggat
    12721 ggactacatg cagcagagtg ggctttaggg gggatttttt cagaaaaaga ttatgtctca
    12781 ctgcttgaag aacagttaaa gacaatcgct gagatcaaag agctatcagt tgaagaagtt
    12841 aaaaaaaata cttatcaaaa cttggaacgt ttcattagat taggtaaata aaaaagagat
    12901 gtgacacaac tagtgtcgca cctctttttg gctttctttg tataaaagta aactgtttga
    12961 ttaataaatg aatcttagta ccaaccgttt gcttcccaga atgctttcgc cgcttcccaa
    13021 gaaccataac gtgaagtaac atattgttct gctactcttt cttggtttgc agcagagtag
    13081 tcaccattta agtaagaaga atctaattgg taacgtccga tgtaacgtcc gttcgttgct
    13141 gtgtaagaac cgcttgattc tttttgtgcg atccattctt ttgcagagct tgtgcttgct
    13201 ggagtagcag cagtttctgt tacagcagga gcaggttgtg tttcagcaac aggcgctgct
    13261 tgtttttccg tttgaaccgt ttcttgtaca acaggttgtt cagcttggac tggtgcttct
    13321 tgaacggctg cttgaggttc agcagctgct tgtgcagagc cttctgttgg gattgttaat
    13381 tgtt
//
LOCUS       DANKKG010000068        13346 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000068 DANKKG010000000
VERSION     DANKKG010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.068"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(209..1045)
                     /locus_tag="PW004_001675"
     CDS             complement(209..1045)
                     /locus_tag="PW004_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HDL2726648.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(1056..1985)
                     /locus_tag="PW004_001676"
     CDS             complement(1056..1985)
                     /locus_tag="PW004_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDL2726649.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            2177..2530
                     /locus_tag="PW004_001677"
     CDS             2177..2530
                     /locus_tag="PW004_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDL2726650.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(2604..3260)
                     /locus_tag="PW004_001678"
     CDS             complement(2604..3260)
                     /locus_tag="PW004_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HDL2726651.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            3486..5420
                     /locus_tag="PW004_001679"
     CDS             3486..5420
                     /locus_tag="PW004_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDL2726652.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(5506..6729)
                     /locus_tag="PW004_001680"
     CDS             complement(5506..6729)
                     /locus_tag="PW004_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HDL2726653.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(6723..8621)
                     /locus_tag="PW004_001681"
     CDS             complement(6723..8621)
                     /locus_tag="PW004_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HDL2726654.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(8615..9733)
                     /locus_tag="PW004_001682"
     CDS             complement(8615..9733)
                     /locus_tag="PW004_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HDL2726655.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(9816..10649)
                     /locus_tag="PW004_001683"
     CDS             complement(9816..10649)
                     /locus_tag="PW004_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2726656.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(10642..11559)
                     /locus_tag="PW004_001684"
     CDS             complement(10642..11559)
                     /locus_tag="PW004_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2726657.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(11786..12910)
                     /locus_tag="PW004_001685"
     CDS             complement(11786..12910)
                     /locus_tag="PW004_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL2726658.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            13093..>13346
                     /locus_tag="PW004_001686"
     CDS             13093..>13346
                     /locus_tag="PW004_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDL2726659.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIII"
BASE COUNT         4184 a         2674 c         2192 g         4296 t
ORIGIN      
        1 ttcatacaac ttttcaacat acatgttagg aataaattgt aattctcttc acaatctttt
       61 ccaacacctt tgaaaccgat atcaaatact ttcaggatgc tgcttctaaa ataaatatca
      121 aacaaatgaa tacatcatgc aaaaaaagcc agacaaccga cgtccttgcg tgtcagctgt
      181 ctgagctatg tttatttatt ttgttctttt aaaaagaacg atacacatac tgatttttcg
      241 gagcagagac tttctcagcg tttgtaacaa ctacagatgg gatttcacgc ttgaatggtt
      301 gataatagtc tgattggatg gttccttcta ttttatacca ttcatcattt ttaaccgtta
      361 ctccttccgg catatgcgtc aacaaaccaa agactcctga atctgccaca caatggataa
      421 tgccgaatcg gaacaagaag atatctgtat tgctatcgtt cggtgtttgg taaacaaatc
      481 cttcatagct gatttttttg ccaatgaatt cgctagggta attataaatt agctccataa
      541 cttctaaata gttttcatct gtgatcttga tagtttgctt atccccatac ttttctttca
      601 actcattcat ttggtctaag tagtctgatt tgttgaaata aatactggta tccggcttta
      661 agtattgtgt ctgcatctcc ggatccccta ctgattcttt acttactggg aaattaaatc
      721 ctttggcttc tacgatcgta gtgtcgagac taactgttgg aaacaacgtc cccacgatta
      781 ctggcaaagc tagcagtaca taagcgattc cacgttgata aggttttgac aacccatggt
      841 catgatcgtg ctcgtgtgcc ttttcttctg cttttccgtt tttgacccac aaatacagct
      901 gaactaaagc caaaagagca gataaaatca ttgaaagaat agctaagtag cgataatgga
      961 cattgatata ttgatcgagt ttgccagaaa cttgcaagta catcatcaat tcgaaatacc
     1021 cagataaaat caaaaaccgc agcatatttt ccctcctaaa taaataatgt gaatccaata
     1081 acgctaaggg caatcagtcc gatcagcgat aaaataaagc gtcctgtaaa atatcgtttc
     1141 atcatcagta gatttttgat atcgaccatt gggccaaaga ccaaaaaggc agctacaggt
     1201 gctgtgccga acagacttaa caaagaagat cctataaacg catctgcttc tgagcaaaga
     1261 gacatcgtaa aggccaataa cagcatgatg ataatggcta aaatctttgt tgagccgagc
     1321 gttagcataa ttctagttgg taaataagtc tgcatcgcag ctgcgattaa tccgccaatg
     1381 atcaaataac gacctgtatc aaaaaattcg tcgattgaat gtgtcaacac atgcccagtt
     1441 tgatggatca gctgattttt tggttcattc ttctcacttt gttcttcttc atggacatga
     1501 tgatggtgat tttctgctac tttagacgtg attttttctt tcaatacagc gtctttttga
     1561 aaataggcaa gccagacacc aatcacaagg gcaacaaaaa agctgcctat cacccgccag
     1621 cttgccatct gccaagaatt gccaaatgcg ataaaagtgg aaaaaatcac gattggattg
     1681 atgatcggag cagtcagcat aaaagcaaac gctgtgtgaa ctggtacgcc ttttttaaca
     1741 aattgatgaa cgatcggtac gatgccgcat tcacatgaag gaaagaaaaa tcctaagatc
     1801 gaaccagtaa aaatagagaa gatacgattt tcaggaagcc atctcttcac acgttctggg
     1861 gtcaaaaata cttgtaaggc gcctgagata agacagccta ataatacgaa aggtaatgct
     1921 tcaataacga tcgataagaa aatcgtactc atctgcaaaa ccgaatttgg aataaaagaa
     1981 aacatgagtt tcctcctttt tatttctagt tcattcgctt tgcaaatata cgcttttatt
     2041 caaaaaaaac aagttgctct gtaaattttt acacaactct tattttttac caaagcactt
     2101 caattgactt tttctgttat tcccattaaa ttaaggtctc atacatatca cttctatcat
     2161 aaaagggaga ataaaaatga aacaaaccac taacttgact gaagttcaag acatactgca
     2221 acagtcatcc gttgccgtca tttatctttc gatgcctaat tgcagtgttt gtcacgctgt
     2281 tcgtccacga ctagaggaac ttctcacaca ttatgatatt ccggcacttc atttggatgc
     2341 atttgaaaca ccagaagtag ctagtaaatt tgaagtgctg actgcgccag ttgtcctgat
     2401 ttttcatcaa ggaaaagaag tctttcggca agcacggttc atcgacttta aaaaaatccg
     2461 ttatctacta gatgaactga cagcagaaga tgattccctt tcttatgaag aaatcttccg
     2521 aacaaaataa tcgttgtcca aacagaaaaa agcgattgaa caagtgaaag ttttgccttc
     2581 atttgttcaa tcgcttttta tttttattct gctgtatttt cggaatgcgc atttgcgtca
     2641 gaatgcaaga aatagatcaa ggtttgcaat tcattcgtca aatcaacatt ttggatcaat
     2701 acatctgacg gagcattcaa tcgagcagcg gtaaagttca agattccttt gattcctgct
     2761 tccgttaact gattgactac ttcttgtgct ctttcagcgg gtatcgttaa aattgctact
     2821 tcgatttgtt ggatcttgat ttgttctgac atatcttcca ttggataaac ggggataccg
     2881 tctacgatgc gcccaacgat ttcctcattc acatcaaaag cacaactgat acgtatgcta
     2941 ttactttgat ggaatttgta ttttagtaaa gcacttccca agttccctac accgatcagc
     3001 gcaacatttg tcaattgatc gtcgtttaat gtctttgcaa aaaagtgcat aagatcttct
     3061 acatcataac catacccacg ttttcctaat tcgccaaaat aagaaaaatc tcgacggatc
     3121 gttgcactat ctacttgaac ggcttcactt aattcagttg aagacacttt tttctttcca
     3181 gcattattta aaatccttaa atagcgataa tacaaaggca gtcttttagc tgtcgccttt
     3241 ggaataatat gatctttcac gaaatgacct ccacgtattg tctaattaca tctttgattt
     3301 ctccatcata gcagtattta taggaaaaaa tcaattccat tgctcagata ttgtgaaatt
     3361 ttattcttta ttcatttatt tatcatttct gcaaatctcg cagtataatg gaatttatgg
     3421 tagactacta attgaaaaat agttttttat ttgaggagaa ttcacaaagt agaaaagagg
     3481 attccatgat attattacaa gccaatcaga ttgctcgcca ttttggtgca gaagtcttat
     3541 ttgaaaacat acatttagaa atcagtacag gcgctcgtgt cgctttagtt ggcagaaatg
     3601 gcgctggaaa atcgacttta ttaaagatca ttgctggaat tgaagcacct gatgaaggaa
     3661 agatcgctaa aaataaaaca gcgactctcg gatacctcgc tcaggacaca ggacttgatt
     3721 ccaatgaaac agtttggaac gagatgctga aagcttttga acaagttaga caaatggaag
     3781 agcggatgcg cgagctagag attttcatta gcgaaggtac gccagacaca cctgcttata
     3841 gttcttattt aaaagaatac gaccggctgc aacacgattt ttcagatcga aacggctatg
     3901 gttatgaaaa tgaaattcgt tccgtattgc atgggtttaa attcgattct tcattttatg
     3961 atcagaaaat cgatacttta tcaggcggac agcgcacacg tttagcttta gctcgcatgc
     4021 ttttgcaaca ccctgacatt ttgattctag atgaaccaac gaaccattta gatatcgaga
     4081 cactttcctg gcttgagaat tatctgcaaa gctatactgg tgcattattg attgtttccc
     4141 atgaccgtta ttttttagat aaagtagtca atgaagtcta tgaattaagc agaagaaaaa
     4201 tgcgccatta caaaggtaac tattcgaaat atctagattt gaaagcagaa cagttagcaa
     4261 gtgaatggaa ggcttatgaa aagcagcaaa cagaaatcca taaattagaa gacttcgttg
     4321 ctcggaacct tgttcgcgcc tctaccacaa aacgagcgca aagccgcaga aagacattag
     4381 aaaaaatgga ccggctggat cgtcctcaag gggatgaaaa acaagcaaac tttctattca
     4441 gtatagaaaa gccttctgga aatgtagtct tgcaaatcga agatgccgct atcggatatg
     4501 ataatgtcac cttatctgaa ccaatcaatc tagatatccg tcgtcaagaa gcgattgctc
     4561 tagtaggtcc gaatggtatc gggaaatcga cgttgctgaa atcaatcatt caccagatcc
     4621 cttttatccg tggaaaagaa acgctaggga caaatgtcac catcggctat tacgatcaag
     4681 gtcaagctga tctgcataca aacaagacga tcctagcaga attgtgggat gagcatccta
     4741 ccactccaga aaaagagatc cgaagtgctt taggtgcttt cttattttct ggcgaagatg
     4801 tagaaaaacc aatccctctg ctaagtggcg gtgaaaaagc acgggttgct ttagccaagt
     4861 tagcgatgaa caaagaaaat tttttgattt tggatgaacc aacaaaccat ttagatattg
     4921 acaacaaaga agtattagaa aatgcattga tcgattatga cggcacactg ctttttgttt
     4981 cccatgaccg atatttcatc aatcggatcg cgacaaaagt cgtcgaactc tctgaaaatg
     5041 gcagcaaagt ctatctagga gactatgatt attatttaga gaaaaagcag gaagaagaag
     5101 aaatcgccga attgttggca caagatgagc cagaatctgc tcctgctccc acgaacaaat
     5161 ctgattatta ccaaagcaaa gaacagcaaa agcttatccg tagccttcaa cgaaaaatta
     5221 ctcaggttga agaagaaatg gcgcggatcg atgaatggat cgatcagttg gaacaagaaa
     5281 tggtcgcgcc agaaaacctg gaagatcacg taaaactcaa tgagctgaac caagatttag
     5341 aagctgctag acaagatcaa gaaacaaaat tgacggaatg ggaagaacta agcttggaat
     5401 tagaagaata tgaaaattaa cgaaaaaacc aaaacagaaa ccattcatgc tgttttggtt
     5461 tttctttttt tattttatca aacaaaaatt ccatatttac ctttttcacc aataagaaga
     5521 ccaattacta gtcattctca ctaaagcttc taaataatag taatcacccc aaatacaagg
     5581 tgtatttacc ccattatttt tgggcttatt ataaacagac tgtgttaaaa agccatcttc
     5641 ttcttccgtt gtagcatatc tttctttcaa ttgtcgcata attttccaaa cagcagactg
     5701 gtataagttc ttttctgttt tgtctaatag acctgattct attaattcta ataatccaca
     5761 aaccatgatc accatactag atgtatcttt ttcttctctg ctagaatcat caaagatcaa
     5821 atcccaataa caaatggtat cttctggcaa ccgattcata aaataattgg ctaatatttt
     5881 gcttaaatca aaatattttt gttctttcaa ataacgataa cttaatgcaa aaccgtaaat
     5941 cccccatgct tggcctctag cccaacagga attatcacta taaccttgat gcgtacttcc
     6001 atatcttggt tttccatctt tgacatccat ataaaatgta tgataagttg aaccatcttc
     6061 tcttactaaa taatctgctg cattttgaat atgagcctga gcagcttttt tatatttttg
     6121 atttccagtt tcttcactcg cccaatacaa taatggaaga ttcatgcaac aatccatgat
     6181 cattctacct tgttgtgaag gatcactagg atctccccaa gcttgtataa ctcctgcctt
     6241 ctcccaaaaa cgctgaagta atgtttctgc agcatcaatt gaagcttcct tcatttttcg
     6301 atttcccgtt attttataat ctgctacggt tgataaagaa tacaaaaaac caagatcgtg
     6361 atgattagtg ataatttgac agtcaatcct gtggataaaa tctatatctg ctttttcagc
     6421 tgtttgttga aataaaggat cacccgtctt ctcaaataac aaccaattaa ttcctgtcca
     6481 aaatccttct gtccaaccaa tattgtctct taatggataa cgtcctttta taggacactc
     6541 gcttgggaat tgatactgac tgttactgat acgtttgatt aacctctccg tgtgtctgcg
     6601 acaacgctcg atctcattat tccattcttt ttccattaat tctttataac attcatttat
     6661 ccatttttct ggatgtatta tctcttcaaa catcacttta tcaagcattt ccccaccagc
     6721 ctttagcata attttagtct ttttttattg tttttatttt ggatagttag cccggttgtc
     6781 ccatcaaaat caattttggg acgattgttc catacttcta aactataatc ccgatcttga
     6841 tgagcaacca ctcctgttcc gagccaatgg gttccttttc caaatgtacc ctctaatcca
     6901 ggaataaaag tgttttccca tgtcatcaag tttgtattgg gcaaacccat aatagctgtg
     6961 gattttcgtt taatagtatc gtcacttaaa cagataatac ctgaaaagcc agcttcactc
     7021 accctatagt tttccttgtc ttcttctatg attattcctt ctttctcagg agaacattca
     7081 atagcaaacc ctgtctcata tagaacatat tctgtatcta attctacttt gtgtacgcga
     7141 atctgccagc cttcaaaagg aataagccaa gttctgattt ttgcctgtaa agtaggttcc
     7201 catgtagagg caagtacctc tttcttacat acaatgtctc gtacattatt tcgaacgatg
     7261 aactgtccat aggttttgat gctctcattc ttaaaactgg tcgtgatata tgtgtccgct
     7321 cgctgaatcc ctagcattga atccattgcc atttctgctt tcaattgatt cgctcgcggt
     7381 atcgaaaaac caaattgact agaataagca aattttaaat atttttcatt ggtcaatgta
     7441 tggaaatttg gccctaattg tcccgcattc aaaaaaaatg tatgtccatt atcatgttgt
     7501 atgagcaaat tggcttctga aagaagttta gttccttcac gaataggcat aggcatttct
     7561 ttagccgtcc aatactcatg gtcttcaggt agcgccaaca ataaaaaaat cttattcagc
     7621 cataatggag ataacgtact attatagggt tcagtcaatg tcaattgagg gtaggtataa
     7681 ccaatattca ggataccttt ttcatcaaat atttcttttt gcatccacca tcttatattc
     7741 cgattgacta accccttgat ttcacctaat ttaaaaggca aaagatcggc ccacaacata
     7801 gctgaccaaa agcttgaaac tgcaaaacga taaatcatgc ttcgaccaaa aggcacattt
     7861 gcaccatctt ccgcaaaaaa atgaatatag tctctagaaa aatcaatagc tcggttctta
     7921 tatatctggc taatgggctt ttctggttct agtacgctat aaatcaaacc ataataatga
     7981 aatgcaaaag gattataata atcttggcga cctcttgaac tgtctcgata ccagccattt
     8041 tgaagataac aattttctat attatcaaat gcttctttca gtgctgattc tttaactgga
     8101 taaccaaatt tttggataat tgttccgaca attatcttaa aaaattgcca attaccatct
     8161 gcacattcat attcaaaaat ttgcgagaac caatgaataa tctggctttt ttctcctgta
     8221 tctaattttt tccataataa ttgttcatga tgatataacg ctaataacat tgcgggcatt
     8281 tctaccatac gctggtctct ttcatgtatt tctccccaat aatcgggatg ggaaggatct
     8341 gttccagctt ttattcctga tagaattaat ttcaaccatc gttcatctaa ttggtcaatt
     8401 gcgggccccg ctccccataa aacccgagag aatccttcca ttcctgcaat tttatttcca
     8461 tatcctaccc cttgatgtat ataatcaatt tgtgcgccac ttgctcgata aaacggctgg
     8521 agtggttgat ttatcttttt aaataagtca aagtaatcct gtctggtcct taatagtggt
     8581 actggatgtt tcattgtatt ttctcctctt tttactacca aatagctaat tccgactgtt
     8641 ctaatcgaag taagccttca ataaaaaaat aatcagaata gatcagtggc acattataag
     8701 tcagttctct atggtatgct tctgctccgc ctccaatgat cccatcttca tccatattcc
     8761 aatttgcata gtgcttttct gtcttttgga gtatattgat agcagcttca tgatacattt
     8821 ttgatcgtac agaggaaaca tatcctgcta attccagaag accgcatgca gcgcatactc
     8881 cagcactggt atcaatattc gaatctttta cagcaaaatc aatgagagga atataaccag
     8941 ttttttcgat tttctgaata aaatttactg ctatcttttc tgctatagct agatagtacg
     9001 gattttttgt gtaacgataa cttaacgcga atccatataa agcccatgca tttcctctgc
     9061 tccaacaaga attctcattg tatccttgcc cgccaagtaa ccgaatatat ttccctgatt
     9121 ctgcatcaaa ttctgcgata tgcccggttg atccatcttt cctcactaga tacttagctg
     9181 ttgtatctgc atgctgccga gcaattttag agaacctcgg atcaccagtt tccctcgaag
     9241 cccaatacag taatggtaaa ttcatcatac tatcaataat catccaacca ggtttatggt
     9301 cattccatgc tactaaatag ttccctgaaa tattatagcg tccagcaagt accgtagctg
     9361 catgtaaccc tagttgtcga ctatgtttgt tttgagactg ttgataatgt gcaatagccg
     9421 atggtaagaa taaaaaacct acatcatgat gaacgtctga aaaatggtac agggcagatt
     9481 ctaataattg ttcttgttgt attgctgcct ctttgaactt ttctcgtttt gtcttgtgaa
     9541 agagctgcca taaaatacca ctccaaaaac cattggtcca ccagtcgatt cctctgtcct
     9601 ttattacgtc tgtatatttt ccgttgtaag aaatatatgg aattttattc cccattctgt
     9661 ctaattccgc atctatcttt tcagaagcta cattcaaaat ttttcttgac cattccttcg
     9721 tctgttcatt catggatatc ccgccattct tttaattttt atgaatgtgg agttgctcct
     9781 agaacgaact agatggttaa cattcgttat tttttttact tatttgcagc tccaatgtca
     9841 ttctccttaa atttagactg cgcccacaca taaatcaaga ccattgggat cactgaagtt
     9901 gctaaaatta gcataattgc gccataatta attggctcac ctgtactaga aaccgtcata
     9961 tttttgataa ttaagggaac cgtatattta tctttatctg aaagcatgac taatggaaat
    10021 agatattgat tccaacttcc cataaagata agtaaaccac tagtaataat cgtaggtgat
    10081 aaaatcggta cagcgatttt cgtaaaaatc acccactcat tggcaccatc gattctggca
    10141 gcctccatca gtgctgtagg aaattgactg ctatattgtc gcatcataaa cactgaagtt
    10201 gtactggcaa tcgaagggat gatcacacct gcataagtat tcgataatcc taaacgactc
    10261 ataatgtaaa ataaaggaat caatgtggta aaaaaaggca ccatcctaga tagcataact
    10321 gtattgaaca acagtttatt ccccataaaa cgatactttg ctaatgcata acccgccatt
    10381 gtagaactgg ctgtagctaa aactgtactg attacagcaa taacaatcga attccaaata
    10441 atcaagggat aattatatct cgccatgatg gcttgccagt tttttaatag catatcccca
    10501 aacttgaaat tgattccgcc acttaaaatt tcttgattac tttgcgttga agctacaatc
    10561 atatataaat atggcatcaa aaatattaag gcaccaaaca caataatgac gtacgtaaaa
    10621 attctgcttt gtatcttttg tttagtcatt tttatcacct ccgaatttaa attgaatagc
    10681 cgataatata acagatacaa acaccatcgt ccaggcaata gcagaggctt ttccaaaatc
    10741 aaccatttca aaacttgttt tatataaatt gactcctacg accaaagctg aattttctgg
    10801 tccattggaa gtcccaaaga atatatttgg gatattgaaa ttacccaagc cattgatagt
    10861 ggctaaaact gtggccatta aaatgattgg ctttagcatg gggatcgtta ataaaaagaa
    10921 ttgccgaaat ttcgatgctc catctatttc agcactttcg tatatctctt tagaaatccc
    10981 ttgaagacca gctaaaaata aaatagtgtt atatcctaca ctaagccaaa tcatcgtgat
    11041 gatcattgat attcgtatcc aaaaaggatt tgttaaccac tgtacaccgt taattcctag
    11101 cgcattcaaa aatccattga ataacccagt cggattgaaa aaagtcatga atacggaagc
    11161 tacagccacc gttgatgtaa tggttggaat ataatagaca gttctaaaaa atccttttcc
    11221 ttttaactgt gctgaattca ataaaacagc aaagtaaata gctaatagca tcgttattgg
    11281 aattgatcct gccatgataa tcaaaacatt cgtgtaagat ttaataaaca atggatctcg
    11341 gaagatagct agataattgt caaatccaac aaattcaaat gagtagccaa ttttcataaa
    11401 actaaaagca aacgagataa tgatgggaat caaaatcgtc acaataaaga aaataacaaa
    11461 aggtgtgacc aatccataag cacttttttt atgagacgta tctgttgctt ttttcttgct
    11521 gttctttatt gtcttgcttg gtaacgaaga ttgcatcatc gttaagtcct cctaatattt
    11581 tttaatcacc agttaatttt acgccgtact tatgagcaaa attttctgct tcttttagat
    11641 atgaattttc aaaatcacct gtttgccaat agttatatgc agcggtagtc aaataagacg
    11701 tagcatctga ataagtggct ggtaaaacag tatttgatgt attttcattg attttattca
    11761 atagttcata gtatttttgt cctccttagt agtcaaactt tttctcagct gcttctgttt
    11821 gataggccaa tgagttagat gtagccacgc ccagctccag tgcgttggct aagcattctt
    11881 catctgtcag cataaatgta agaaattgtt gtgccacttg tggatttttt gcatctttag
    11941 ggacataaaa acttgatcct cctgatactg caccttttcc tggattttct tttgaaaaag
    12001 gaatcagtgg agccatgcgc catttgtcag atgcatcagg aaaattcaaa gacatattcg
    12061 tagcatacca gccgccagca acaaacattg cagattcttg aaatgctttc tcagcatctt
    12121 gtgcaccgta gaaatgtaca atatctgagt cgatcatttc tttgattaac tttgcagcat
    12181 gtttcgcttc tttagtattc agacggatat ttccttcttc atcaagcaaa gattgattct
    12241 gctgggctag catgttgata aacaattctt ggtccccaga ttctggcaag gcaattccaa
    12301 aactaccagt tttttctttt aatgcttttg ccacctggat atattcttcc caagaactga
    12361 tttcttcaaa atctacacct atttcagcaa atttttttgg ttgataataa caaagtgctg
    12421 gggtaaaatc actagcccaa ggaataatcg tttcattact cgtttgttga cgcaaaatat
    12481 tcaatttttg atcataaaac tgatcgccat atttgtcata aaatccatat tcatcagcac
    12541 tataaaaagc atcttgaaat ttttccagca cacctgctgc tgaagtgtct gccatggaga
    12601 taatatccgg catatcttcg tgtgttacca attttggggt gatcatttgt actgttgctg
    12661 ttcccgaagc tacattcaca atttttaagt caatatttgc tccagttttg cggttataga
    12721 tttttgcagc attttcaaca tagtttgtca gtttgccgtt tgcgacatat acttctaatg
    12781 tagtggcttt ttttatcatt ggatctgcga tgtttgctgt tacttcttcg ttagtttttc
    12841 cacatgcact taagctgaat aatagggtaa tgtttagcaa agctatcacc aatttgtatc
    12901 tttttttcat gtctatccct tctttctttt caagttctta tataaaacgc tttcacttat
    12961 gtatattaac ggaattagta acacaaatat ttatataaaa aaatatttta cttgtcgttt
    13021 tggcttttca tttttgttat aatagtcatt aattataaat agaaagcttt ttatataaaa
    13081 agagaaagga aagtggttct tttgttaaca aacaaccaat cctccaatct tgtcaaaaaa
    13141 acactaacct attccataga tacccatctt catttatctg ctgctcccta ttttcgacta
    13201 ctaataaatg aaagcgctaa tttatatgct gaatttgcac ataaatctat agaactagat
    13261 aatccttccc ttttattcat tgataaacag caagaagtcc aaatcttttc tactcaaaag
    13321 gaaacacaaa cttttatcat tatcca
//
LOCUS       DANKKG010000069        13335 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000069 DANKKG010000000
VERSION     DANKKG010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.069"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            6..152
                     /locus_tag="PW004_001687"
     CDS             6..152
                     /locus_tag="PW004_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HDL2726660.1"
                     /translation="MLVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEENRKKAMRK
                     RVSR"
     gene            complement(214..2025)
                     /locus_tag="PW004_001688"
     CDS             complement(214..2025)
                     /locus_tag="PW004_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HDL2726661.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(2022..2387)
                     /locus_tag="PW004_001689"
     CDS             complement(2022..2387)
                     /locus_tag="PW004_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HDL2726662.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(2550..2945)
                     /locus_tag="PW004_001690"
     CDS             complement(2550..2945)
                     /locus_tag="PW004_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDL2726663.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(2963..3925)
                     /locus_tag="PW004_001691"
     CDS             complement(2963..3925)
                     /locus_tag="PW004_001691"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HDL2726664.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(3925..4137)
                     /locus_tag="PW004_001692"
     CDS             complement(3925..4137)
                     /locus_tag="PW004_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HDL2726665.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(4158..4856)
                     /locus_tag="PW004_001693"
     CDS             complement(4158..4856)
                     /locus_tag="PW004_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HDL2726666.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(4876..5418)
                     /locus_tag="PW004_001694"
     CDS             complement(4876..5418)
                     /locus_tag="PW004_001694"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HDL2726667.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(5550..6554)
                     /locus_tag="PW004_001695"
     CDS             complement(5550..6554)
                     /locus_tag="PW004_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL2726668.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(6551..7540)
                     /locus_tag="PW004_001696"
     CDS             complement(6551..7540)
                     /locus_tag="PW004_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL2726669.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(7537..8343)
                     /locus_tag="PW004_001697"
     CDS             complement(7537..8343)
                     /locus_tag="PW004_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2726670.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            8509..9465
                     /locus_tag="PW004_001698"
     CDS             8509..9465
                     /locus_tag="PW004_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2726671.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(9542..10060)
                     /locus_tag="PW004_001699"
     CDS             complement(9542..10060)
                     /locus_tag="PW004_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDL2726672.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(10148..10297)
                     /gene="rpmG"
                     /locus_tag="PW004_001700"
     CDS             complement(10148..10297)
                     /gene="rpmG"
                     /locus_tag="PW004_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDL2726673.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            10525..10971
                     /locus_tag="PW004_001701"
     CDS             10525..10971
                     /locus_tag="PW004_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HDL2726674.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(11166..13061)
                     /gene="asnB"
                     /locus_tag="PW004_001702"
     CDS             complement(11166..13061)
                     /gene="asnB"
                     /locus_tag="PW004_001702"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HDL2726675.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         4149 a         2831 c         2275 g         4080 t
ORIGIN      
        1 ctggaatatt agtaggaagt ctggcaacag ccgctgccgt tgctggtttg gttgcttctg
       61 tgaaaaaaac agtgatcgat ccaattgacg aaaaagaagc aatgatcgaa gaaaaccgta
      121 aaaaagcaat gcgtaaacgc gtttctcgtt aataattatt taaaaatacg catgaagact
      181 ttctaaaaaa cagataagtc atcatgcgta tttttatttg tcagtcaata taagttgtgc
      241 tattttatct gcctgtctca gattccatgg atctgtataa ggtctagcta ctgctgcagt
      301 tagccaatcc acaccttcaa caggtattcc atagcaataa aagtcagact ggatttcccc
      361 tgaagcatca atcacgcgat tcgtacctcg ctcaacatct atagcttctg aatgataact
      421 tcctgtatta tctgaaaatg aaaaagggcg aacaagtttg ttttccttca aattttttat
      481 gacaggatta cttgaacgag aaaagcttgt ttgtggcaat cgagcttcaa tcaaatagcg
      541 actgctgatt tctttatctt gtagagaaat aacatagcgg ttgtgctttt gctgaatatc
      601 aaatggcggt tcgatcaaat gaaaaatacc cgcatctatc agtgctgcta attctttcgt
      661 ccgaatgatt ggcggaccga tcgttaaaaa gctatttaga tgagtaaacc atccccatag
      721 tatttctttg tattccttag cagaaaaaac gtgccattca atagcctgcc ggattggatc
      781 acgccagtct tttaatgcat caaatgttga agcaaatgct cctgtgcaat tgcctttttc
      841 cgcttctttt atctgccctt caataaatat tcggctagct tctgaaaagg gctcattctg
      901 ataggcaaga ggagtttcta aatacgacca atcccatcta taagggatta gtgctggatg
      961 ctttccgaga cactcctctg aagagaggga gagaaaatca tgctcaaaat ttttctttgg
     1021 aatcggcaag tgatattctt cgatgatttt cttgtaataa aacaactcaa cgtctttttt
     1081 cagatagtca aaaaatgttt cgccagtcaa aaatcccgac tgatgccatt gctccaggtt
     1141 ttcttttgtt agtaaccgtg gccatgccat tgctccgcct tgcttttgat ttcttcctcg
     1201 agggtaataa gggagccccc ttcttgatcc actgtataca atcggttcct tgcctgatgg
     1261 atgatagacc agttttccct tttcgttctc gaagcgccct cctcttgata ctgtaaacaa
     1321 tccgacataa tcaaaaaaac ttaagcctag acctcttaaa aagacatttt ctttaggcgg
     1381 tatcttcgaa agatctacat ctgcaggatt agaaggaagc tgatacagta aaacattttt
     1441 ctttgcgtag tctacccaca ctctttcttc ttctgtctca tcattttccc agtgaccgct
     1501 tgctaaaaca agctgatcga tagaatagct ttgctgatct gtctgtaaaa gataaccatc
     1561 ttttcttttg cttacttttt ttactaaaga ataaacaaac tgaatagaag ctggaaattc
     1621 ttcttgcaac tttgagaaaa accagcgttg atagagtccg tataaacatc gagtagattg
     1681 ctgatctttt tccagacgat tgatttcttc tataattagt tgcttatttt tggttaccgt
     1741 ctgttcgata taatctgtag caggtccttt actccattga aacaagttgg gtccctgtgt
     1801 taccaatcct ccactggaca atgtttcatc tgtaaaaagc gtcacttgtt gagaaacaga
     1861 attcattaat aactccggtg gctgattcaa ccgccaaact cgacctccag gaccattggg
     1921 atcgaaaaga aaaagttcaa gagaatgtct gtcctgatta ttatggagaa tcctttctgc
     1981 tacacttaat cctctaggtc cggcaccaat gatccctatt ttcatttgca tgcctccatt
     2041 tcgataaatt ttttcaataa aacagcatgg ttctgatgtt cattttttgc accataaaca
     2101 aaagtaatga ctggatgttc tttcataagt tgactgagtt taaaaatagc ttgcttcttt
     2161 tcttcttcga aaatcaattc ttgctggtat ttctttgcaa atgaagcaaa tcgttctggt
     2221 acatggtcaa aagttttgcg tgcttcatta ctaggtgcaa tttctttcaa ccacagatct
     2281 aactgcgctt tttcttttga aattcctcgt ggccagaatc tatccacgag tattctatag
     2341 ccatcctctt tttcagcatc aaggtaaaca cgttttaatt tgatcatagt tgtttccttc
     2401 tttctacctt ttcgcttagc ttatatatgt agataatagc acaaaataaa aaaaatgaac
     2461 ggcacaacta tccattgcgc cgttcatgtg atcttggtca aaatctaatg attgattcgg
     2521 tttttaaagc tgtcatacaa tcatctgttt tatacttttt tacttttgat ttttccagac
     2581 catccgtcat atccaccctt aagtaaataa atctcttgat aacctgcttt acgtaattta
     2641 tttgctgtac ggatgctcaa acttttcttt tgatcgtaaa tatagacagg ctggtctttt
     2701 cgaatagatc ctaatgaatc tttcaaaaca gtgtaaggga tgtttcttgc acctaaaata
     2761 tgcccagcat caaacacatc tttttcgcga acatcgatta cttgtgcttt tctcattcct
     2821 tctcgaaatt cttcttctgt caaagtcgtt gctgaacgtt tggccattac tcgtagatag
     2881 acttcccaaa ggatgattgc aagcacaatc aatagcaaaa tgatattaat tacccataaa
     2941 accatgtaat gcctccgttt ttttaagcaa atttcaaggc taatgaggca gcaccaatga
     3001 caccagcttc attaccaagc tctgctaatt tgatttttgt tgattctctt acttgcggga
     3061 aagtaaattc tttaaaatat ttctctacac ggctacgtaa aaattctcct gctgccgata
     3121 ctcctccacc taacacgata cttgacggat ttaatgtatt ccccaagttt ccgcaagcta
     3181 aacctaagta gaagcatacg cgatcaacaa ccatcaatgc aaacggatcg tcttcttgag
     3241 ccatttcaaa gatatcttta cttgttactt cttctccgtt gtctagcatt gctttcagtt
     3301 ttgaatcccc tgcgtattct tctgccaaga aacgacctag gcgtaccaca cctgtagcgc
     3361 tagatacagt ttctaaacat ccgcgtttac cgcaagtaca ttcgaaccct tcaggatcca
     3421 cagtgatatg tccgatttct cctccagctc cgctcacgcc gtgaatcaaa tttccgccag
     3481 caacgatacc tccgccaaca cctgttccta gagtaacgaa tacaacgtct ggatcgtttt
     3541 ctccagcacc tttccatcgt tcacctaaag cagcaacatt cgcgtcatta tcgatagcga
     3601 atttgattcc tgttcctgcc tcgatcagtt ttttcacaaa ttgaacttct gtccagttta
     3661 agttatacgc accaataacc gttccagctt cgctgtccac gcttcctggg gtacccatgc
     3721 cgattccgat aaagtcttcg gctttcatat tatataaatt caaacgatga ttgattgatt
     3781 caatgatttc aggaacgata tgcttaccat catccaaaat attggtatcg atgctccatt
     3841 tttgctgaat ctctccttct ggtgttaaaa ttgcaaattt tgctgttgtt ccacctaaat
     3901 caatcccaat aatcttcttg tccattacaa atttcctttc tttcggcttt cttcaattcg
     3961 gtgctctctt tttaatatgc gccacgctgt catgtatgta tcatgatcaa tcaaacggct
     4021 atcatacaga ttacgcaatt cgatagtcat taattcaatg tcatagatgc gtgcacccac
     4081 atagataaag atgccaaatt ttttgaacag ctgctgtaca tcatataagc tctccatctt
     4141 taagcacttc cttcatatta tacaagtaaa ccatattttt ttaaacccag taaaatacat
     4201 attacaaaaa gaaatacaaa aactagtgta gaaagaatcc tttggtgtat cgaatagctt
     4261 gtctgttgtt tcggtagacc caaaacattc cctaacacta atccgccaat tagcccaccg
     4321 acatgtcccc aaacatctac agaggtatcc agaagaccaa aaacaaatgt caacacaata
     4381 aataaaagaa aatgtctgac tagctgctgg atcgctgggt tatgtttaaa atgaaaccct
     4441 aagataaaca acgcaccgaa caaaccgaat atcgaagtac ttgcaccacc agacagaaca
     4501 ttcgcttcgt taaaagcaaa gcttgctgta tttcccatta tcccgctgaa catataaatc
     4561 aagaaaaaac gccaatggcc atagattgcc tcgatttgct gccccataaa atacagtatc
     4621 agtgagttca ttacgaaatg catcagtcca aaatggatga agattggtgt cactaaacgc
     4681 caccattcat tgaagtgaac aagataagga ccatacattc cggcataata agggatctca
     4741 agtcgcggaa ataattccat catgaggaag accgctgtct gtatccctaa aaaagcatag
     4801 gtccataacg gtcgtcttaa ccatgcacgc aattttattt gctgttgata attcatgaaa
     4861 acctcccatt tcattttagt caataaacaa ttgttggatc ttctgatcaa agctctccgg
     4921 ttgccattgt tcattcaatt gttcacgaaa aacaaggcta catgtttttt tatgaaaatt
     4981 atttaaatac cggtcgtaat aaccaccgcc aaaaccgatt cgataaccgg ctctattgta
     5041 gataatacct ggtacaatga ggagatcgat catctccggc aaatagagct tctctgattg
     5101 tggctcaaga acaccaaaac tcgaacgtac aagaacggac tgttcatcca tttcataaaa
     5161 cgccatctga cgtttcggca aagttttagg tacaactatt ttcttttgtt ctttttctgc
     5221 tcgttggata accggctgtg tatccagttc aaattcattc gataaagtcg tccctatcac
     5281 ttttgctgac ttccagtact cgctggcgaa tagctgttta tagattcgct gttctttttc
     5341 ctttttcttg ccacttttgg ccagattttt cagcttttcg attcctttcc ggcgtaatct
     5401 tactttcaat tggtccaaaa actcaccctt ccttctctca taaaatggta taggaaaaaa
     5461 agaaaaatag caatcgaact ttcaaataaa tgcataaata gaataaaaag aagacctaga
     5521 aatgattggc cttccttttt taatgaattt caactgttca gcgcgtgttt ttgtctgata
     5581 aaaagtgcat acagaaaata tggcgcacca aaaagggcaa tcaaaattcc tgtaggtact
     5641 tccccacttg gtagaatcac tctagcaacc atatctgcac taagcagaaa aatggaaccg
     5701 aacaaaccgc tcagaacaaa ggtccagtta ttttctttac gtacgactag cttcgcagca
     5761 tgaggagcta gtagtcctac aaaagataaa ctacctgtca ccgctacact accgcttgcc
     5821 aaagcaacag ctaacaacaa aaggattttt tgacttttag ccacgttaag gcctaatcct
     5881 tgcgcgcgtt cttgaccaaa tgatagcaat acaatcctct gtgcataaag tcctcctagc
     5941 ggaattaaga taaccgccca tggaagcaat gcccaaacat aaggccagct cgcaccccag
     6001 attgatcccg ctaaccatgc attcacaaat ccataatttt ccttagataa ttggatcgtg
     6061 catagcaatg ttaatgcaga aaggccggca ttaacggcaa taccggacaa taagattcga
     6121 ttcataccca aaaagccatt tcgatcatag gccgcaagat agacacagca agcagctaaa
     6181 acaccaccta gaaaggctac aactagcaat agaaacggca gcgaatagtg cgaaaagaaa
     6241 cctaaataaa gcatcactaa aaatcctgct ccggcattaa tgcccaatat actagaatct
     6301 gccaattcat ttcttgttac tccttgcaag atataaccag aaacgccaag acaagctcct
     6361 gctaataaac tgatcagtaa tcgaggaact cgaaattctc taacaatcag cgccaaagaa
     6421 gatgattctt tccctatgaa cgtggcaaaa agctgatcca ggtaaataaa aggcgtacca
     6481 atcattaaac tacctataga caggatgaca agtacaaaaa gcaagaaata gaacgtccaa
     6541 tatatttttt tcaccgttgt tccctcctta cttgtatcag taaaaacgga acgccaatca
     6601 atgcaatgac taatccaaaa ggtgtttcaa aagggggatt gatcatcctc gctgtaaaat
     6661 ccgccaacat aaccaatcca ccgccaacta aaaaagagac aggtagaaca taacgatagt
     6721 ccgttccaac cattccgcga acaactggcg gaaccaacag cccaataaat gaaacaggtc
     6781 cgactaacga aacggcaatg gctgctaaca ctgcaactag acagaaaaca atcaaccgcg
     6841 ttcgtttagg atgacttcca agagctaatg catgtgtttc actcaaatga atcaaagata
     6901 ttgcttttcc taatgaaaaa gaaaccaaaa cagcaagtat atataaagga aacaatactg
     6961 ctaattgttt ccatgtgata ttggcagctc cgcctacaaa ccaaaaagca agatcctgct
     7021 gcagattaaa gaattgggta agtgcttggc tgatagcagt aaaaaaagaa ctaatagcta
     7081 cgccagctaa aatcagctgt aaaggattca tgcccatttt cgaaaactgc aaaaaaccaa
     7141 atacgagcaa agttacagca aaagaacctg ccaatgacat tagaaacaca gaaaaatcag
     7201 aagctttagg ccaaaaagca aaactaaggg ccattgccaa cgaagcaccc gcgttgattc
     7261 ctagtaagcc tgaatctgct aaaggatttc ttgtcactcc ttgcatcaat gcaccgctca
     7321 atgcaaaaca actccctaca agaaatgctc ccgccacacg aggcaaacga atatttcgta
     7381 tgatttggtg actttgtttt gatctatcaa aatcaaacaa cgcctgccaa aaatctgctt
     7441 tttccacagt cgctgcgcca tttgaaagag caacaaaaaa aataatcaaa agaacaaaac
     7501 agaccaaaag caggagtcta tttttcttca tagtcctcat aattgatcct cctgctcttt
     7561 tggcaacaaa tcataggtta gaaaaactgg tttatttgtt cttggaagcg tgacaatcgt
     7621 tgcatcaatc tcaaatattt ttttcataca ctcttttgtg atcacttctt caggtgtacc
     7681 ttctgtgatc aacttaccgt ttttcattgc taataaataa tctgaaaaac gtgaagcatg
     7741 attaatgtca tgaatcgaca tcaagatcgt tgtttgtttc atttgattga tttcagtcaa
     7801 taactgtagg atctctaatt ggtgtgccgg atctaaaaat gttgtcggtt catccaagat
     7861 caagatatct gtttcctgag caagcgccat agcgatccag acacgctggg tttgtccacc
     7921 agacagcgtt gcgatatccc gcatacgaaa tccagctaga cctgtagcat ccaacgccca
     7981 ctcaattgct tcatgatctt gcgtgtccat gccactgaac agtttttgat aagggtatcg
     8041 tccgtaagat actacttctt ctactgtcat acctgtaggg tgttcactag tttgtgctaa
     8101 taatgcaatt ttttttgcaa gttcttttga actgtatgta gagatatcag agttattgac
     8161 aatgatcttt cctgatttca caggtagtat acgggtaaaa cttttcaata aagtagattt
     8221 tccacttcca tttggcccaa tcaaactagt gattttccct tcaggaatcg taacagacat
     8281 gttttcaatg atttttttgt tttcgtagcc ggctgcaatg tcagctgctc ttaatctcgt
     8341 catttcaccc atcctttcgc tgaaaatcat tctcaattat gacgctgttt caacatttcg
     8401 tcaagcgcta ctttcatatc tatagccaaa tgaagcggtt gacagcgttc gaaatatcag
     8461 gaataataac caatgagaaa gattctcaat tatgttagga ggaaagaaat gaaattaaaa
     8521 acaaaagtat tcacattatc tagcatattg ctgctggctg gagtactgag cgcttgctcc
     8581 ccctcttcag ataaagaaac agctacaact gccaattcga cgacttcatc caatagtact
     8641 catacagtca cagatacact tggacataaa gtagagattc caaacgcgcc caaacggatc
     8701 atcggcagtt acttagaaga ctatctgatt gcactagggg aaaaacctat cgcccagtgg
     8761 actgttggca gtggttccat tcaagattat ctgcaagatg atttaaagga tgtcccaacg
     8821 atttcttatg accttcctta tgagaaagtg ttaagctttg agcctgattt gctgttgatt
     8881 tcttcttccg ctactgtcga aggcgggaaa tacgaacaat atagcaaaat cgcgccaact
     8941 tatgttgtaa aaaacggaac agatgtcaca tgggaagaac aactaaaaga tgtcggcaaa
     9001 gctttggcaa aagaaaaaga ggcagaaaaa atcatttccg attatcaaaa atccatcaag
     9061 aaaactcgag aagaactagc agataaaatc gagaacaaga cagctgcagt gttgtgggtg
     9121 accaacaact cagcctttat ggtcagcaaa aatcggtcaa gtggaagaat cgtatacgac
     9181 gatctggaat ttggtgttcc tgatctagtg gaagaggtct ccaaaaaagc cacttctgac
     9241 tggtctgctg tttcctctga aaagctagct gaattagatg ctgactacat catccttgtg
     9301 aacagtgaca aagatgctgc catgttcaat gaaccaagct ggaagaatct aaaagcagtg
     9361 acagacaatc atgtactgca attcggacct gaatctagtt ggctatataa cggacctatt
     9421 gccagtcagg aaatggtcaa tgatatcaaa gaaaaactaa attgatttta actaaaaaaa
     9481 gcatcttcac gattagtcgt gagatgcttt ttttcttatt aacaaactag acgcatagat
     9541 tttattcttc ttttgaagca accctatttt ttactctttc ccatttttga aaacgatggg
     9601 catatagatt ttcttgatcc gttatcccct catactcctc tataagggcg aagtctgaaa
     9661 aatcaatatt cccaatatac gtatcgcctt caaacgtgct atcgatcaat gttcgccaga
     9721 tccgaccagt gtaaggaaga agttcttgaa aaatccgact accacccgaa acatagatat
     9781 cttctgagat cgtttgacta tacgacaaaa gttcatcgat cgaatgcatt acttccgcat
     9841 tttctttcgt gcgaaaattt ggttgcgttg tcaacacgat gatatggcga taaggaagtg
     9901 aaagatcccc cattccttca taggtctttc ttcccatcac taaaattcga tccattgtat
     9961 gttcgcgaaa gaaacgcata tcatttggca atcgccatgg taacaaacca tttttgccga
    10021 tcagaccatt tttatcttgt gcccacatgg aaataaacat attatccgct ccttatgatt
    10081 ttagatgttt tgagataaaa aaagtctcaa ttctttgacc agcaaggaat tgagaacttt
    10141 aaaaacatta ttttgtttca cggtgcaaag taactttgcg ttcgcgtggg caatattttt
    10201 gtttttccaa acgatcagga ttgttacgtt tgtttttgct tgttagatag ttacgttctt
    10261 tacaagaagt acactctaaa gtgatgttta cgcgcatgtt tttccctccc atttttacaa
    10321 gaatcctcta agatgactct cagacttgat tatcttacca tgattttttc tagattgcta
    10381 gtgtttttca cgattttgtt aagtaaaact acttgacgat aaatacaacg gttttacttc
    10441 tcaaacaaaa tctttttctt tttgtataaa tacccttaca cttgtttata tagattatgt
    10501 tttttaataa agggggatac taatatgcca tggaacatga aggactttcc tgcatcaatg
    10561 aaaaacttag ataaattgac aagaaaaaaa gcaatcgaca tcgcaaatgc attattagat
    10621 gaagggtacc ctgataaccg tgcgatccca attgccattg atcaagcaaa aaaatggtat
    10681 gaagatgcta gtgaatcaga aaaacgaact ttcgaaaaag aaaaaaatcc aagcaagacg
    10741 gatgaacacg atacgaatcc tcgtgctgga aaactgcttg attcagatgt gatcgtagaa
    10801 tatgaggaag aacaatggat cgtgaaatct aaaggggcaa aaaaggcaag caaccatttt
    10861 gatacaaaaa aagaagcaat cgaaaaagga aagcaagtag cacaaaacaa agaatcaact
    10921 ttgttcattt ataaaaaaga tgggacaaaa gaaaaagaaa tcgattattg aacatatgta
    10981 aaggggttgt gacgaaaatc gtgtcacaac ccccttatgt tcgaaacaat ggtgttcctc
    11041 tgccaactcc gaaccataag tcataatatc tataaaacgg gaagagctgt tttttgaaat
    11101 tgtttcttat cactcagtgt taaacggtag gagaaaacct cttttttatt gatatttgat
    11161 tttcatcaaa cagtcacata gtcgattgct tctgcttgcg gaatcgtttc atctacaaag
    11221 tagactttat accatgtcaa gaaactgaag attgtccaga tttttctttg ctcttcttta
    11281 cgtccttcgt gatggtcatc tagcaacttc atgatttttt cttgatcaaa aaattctttt
    11341 gcaaaatctg cttcgaataa tgctttgact tgttcgtagc cgtgttcttc tttcaaccaa
    11401 gctttgattg gcacagggaa tcctaatttc acacgattcg accattcttc cggaagatgt
    11461 ttgttcgcag cttgtctgaa aatatctttt gtgttgtttt gattcaataa aaacttactt
    11521 ggtatttgtt gagccaattt catgacttcg atatccaata aaggtactcg gacttccaaa
    11581 gaacttgcca tagaaagttt atctgccttt aacaagatat cttttggcat ccattgatga
    11641 agatcgacat attgcatctt attgacttca tctttgattc cttctgtttt ctcgtaatga
    11701 acagtcatga tttcatccac agatggtgct gtttgatatt ttggtgttaa cacttctgat
    11761 gcttctgatt cttcgaacac tcgagcatga ccgataaaat attcttttgc aggagcaagt
    11821 gattcatata aatggatccg tccatggaag tttttcattt ttccgatttt acgtcctaaa
    11881 ttatagcgtg ttccttttgg taattttttc aatccttgag caatcacacg gatgaatttt
    11941 gaattcgtat ggaatccata cgtttggtat cccgcaaaca gttcatccgc cccttctcca
    12001 gattggacta cccgcacact ttgtgaagca agatttgcta aaaagtacaa aggtacgcaa
    12061 gaaggatttg aatcaggttc atctaagtga tactggatca aagggaatgc tttgaacgac
    12121 atgtctccgt cgatcactgc tgctgtatta tctaagtcca atagttcggt tagttttctg
    12181 gcttcaatcg cttcattata ggttttatca tcaaacccga ttgagaaaga atgatctggt
    12241 ttcaatacag aggttacata actggaatca accccgctgg ataagaaaga accaacttct
    12301 acatcactga ccgtatgtgc ttcaatagaa gcttgaacgg tttcgtcgat tttttcgatc
    12361 cattcttgtc gggaatgcgt ttcttttgtt tcaaaatctg catcccaata ttgctgaatg
    12421 tctatttttc catctttata cgtataataa tggccttcag gcaaacgata gacatctttg
    12481 aagaatgttt cgccgtttaa tggagaatat tggaaagtca tataaggttt caacgcttct
    12541 ttgttcaatt ctttgttaaa atcaggatga ggcagaaaac ttttgatttc tgaaccaaac
    12601 ataaatgttc cgttcatttc tgcataataa tatggtttga tcccaaagtg gtctcttgca
    12661 ccgaataact cattcttttc attatcccaa atagcaaaag caaacattcc gcggatcttt
    12721 tgcaataatt ctgttcccca ttcttcgtaa ccatgcaaaa ggacttctgt atctgcatgt
    12781 gtttgaaaaa cgtggccagc tgcaatcaat tcttctctca aaggctggta attatagatt
    12841 tcgccattaa aaataatgat ctttgtccga tcttcattgt aaataggctg agtaccgccc
    12901 tctaagtcaa taatacttaa tcttctgaat cctaaagcaa catgtttatc gatatattct
    12961 cctgaactat taggtccacg gtgaacgatt cgatccatca ttgtattaat aattgttttt
    13021 ttgttgtcct tgtcgttaac aaaacctaca ataccgcaca tttaactttc acatcctaat
    13081 ataaaattac tgtttatttc attcatatgc atcttatcat agaataaata gcggacgaat
    13141 attttctctc ttacaatttc gtcacgtgaa aacaaaagat gaaagtttct gtttttacag
    13201 aaaataaagt cccaatatct aatttaccat gttttctttt ttgaggctac cttattctct
    13261 tcgtgttaaa aaaatattag tggatgctat ttccaaaaaa accagcaaaa aaagacacga
    13321 cctaaagatc gtgtc
//
LOCUS       DANKKG010000070        13331 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000070 DANKKG010000000
VERSION     DANKKG010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13331
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.070"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            66..767
                     /locus_tag="PW004_001703"
     CDS             66..767
                     /locus_tag="PW004_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HDL2726676.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            764..1885
                     /locus_tag="PW004_001704"
     CDS             764..1885
                     /locus_tag="PW004_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HDL2726677.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            1900..3132
                     /gene="pepT"
                     /locus_tag="PW004_001705"
     CDS             1900..3132
                     /gene="pepT"
                     /locus_tag="PW004_001705"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HDL2726678.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            3264..4172
                     /locus_tag="PW004_001706"
     CDS             3264..4172
                     /locus_tag="PW004_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HDL2726679.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            4207..4479
                     /locus_tag="PW004_001707"
     CDS             4207..4479
                     /locus_tag="PW004_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HDL2726680.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            4490..5536
                     /locus_tag="PW004_001708"
     CDS             4490..5536
                     /locus_tag="PW004_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HDL2726681.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            5787..8396
                     /gene="clpB"
                     /locus_tag="PW004_001709"
     CDS             5787..8396
                     /gene="clpB"
                     /locus_tag="PW004_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HDL2726682.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(8547..9233)
                     /locus_tag="PW004_001710"
     CDS             complement(8547..9233)
                     /locus_tag="PW004_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726683.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(9211..9705)
                     /locus_tag="PW004_001711"
     CDS             complement(9211..9705)
                     /locus_tag="PW004_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HDL2726684.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(9725..10945)
                     /locus_tag="PW004_001712"
     CDS             complement(9725..10945)
                     /locus_tag="PW004_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2726685.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            11092..12927
                     /gene="lepA"
                     /locus_tag="PW004_001713"
     CDS             11092..12927
                     /gene="lepA"
                     /locus_tag="PW004_001713"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HDL2726686.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            13123..>13331
                     /locus_tag="PW004_001714"
     CDS             13123..>13331
                     /locus_tag="PW004_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2726687.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDK"
BASE COUNT         4397 a         2411 c         2778 g         3745 t
ORIGIN      
        1 ttttttattc ctagtctcct tattttattt ggaaaatgct ataattatct agaaggagtg
       61 aagctatgaa ccatttagat ttatctcagc gcttagccgc agtgggtgac tttgtccctg
      121 aatccgctcg tttagcagac attggatcag atcacgctta cttacccgtt gcattgatat
      181 taagaggaaa aatcgaatat gcagttgctg gtgaagtcgt tcaaggtcct tatgaatcag
      241 cacgtaagca agtcaaaaaa gatggattag aagaccgtat caccgtacgc cttgcaaatg
      301 gtctagatgc tatccagcca caagatggta ttacagctgt cacgattgcg ggtatgggag
      361 gttcactgat ccgagatatc ctagaagctg gcaagttgaa tcaacgctta agcggacaag
      421 aacgtttgat ccttcagcca aacatcggag agcgtacatt acgatcttgg ctgcaagaaa
      481 atcagtataa aatccaatca gaaaaaatac ttgaagaaaa taaaaaaata tatgaaatca
      541 ttgtagccga aaaagaacaa aaaccagtta cttacaatga aatggagctg atgttcgggc
      601 catttctgct tgaagagaaa agtgagatct tcaaatcaaa atggacaaga gagcttcaac
      661 aaagaaaagc tatccgtgaa cagctcaaac aagcagctaa tcaagaggca cgtcaaaaac
      721 aaatcgagca ggaaatccaa atgattgagg aggttttaaa gccatgacaa tcagcggacg
      781 cactttcatc caaaagtttg aaagctattg tcctcagtgg ctagctgaag aaggagaccc
      841 agtcggactg catatcggaa cattggataa gccaatccaa cgagtaatga tgacattaga
      901 cgttcgtcca gaagtggtcg aagaagcaat caagaaaaaa atcgatttgc tgattgctaa
      961 acatcctcca atctttcgtc ctgtaaaacg gctggttact gatcagccac aagaaaaaat
     1021 gtatgcagat ttgttgaaac atgatattgc cgtctatgca gcacacacga acatggatat
     1081 tatttgggat ggactgaatg actggttttg tgaacttctc ggtattgaag tggagagtta
     1141 tttagtaaag actcatgaga ttcattataa aaaactggcc gtttatgtac ctgtggatca
     1201 tgcacagaaa atgcgtgaag tattagctgc agctggtgct ggtacacaag gtgattatac
     1261 cggtacaagt tttacctcga ttggacacgg tcgatttact ccggaagctg gtgcacaacc
     1321 agcgattggg aaagtaggga agacagaaca agttcaagag gcaaaagttg aggttatttt
     1381 gccggaaact atcgaaaaac aagtgattca agccatgcgg tcagctcatc cttatgagga
     1441 acctgcttat gatcttttcg ctatagatga accagtcgaa atgtttgggt taggacgagt
     1501 aggtgaattg ccgcaagaaa tttctataga ggcatttgta gaacaagtaa aagaagcgtt
     1561 ccaactagat ggactgagaa tagtccagcc aaaaaatgca aaatcctctg tgaagcgcat
     1621 tgcgatttgc ggtggaagcg gtgaaaaatt ttatccgcaa gcaatcgccc aaagagcaga
     1681 tgtctatatc acgggagata tctattatca tacggctcat gatatgcaaa gcgctgggtt
     1741 gattgcaatt gatcccggtc attatattga atcgttatgc aagcaacgat ttatcgagaa
     1801 atttgaatct tggaaacaag aagaaaattg ggatattaac tttttcgttt ctgaaacaaa
     1861 tacaaaccca tttcaattta attaaaagga gagaatacta tgtacgaaaa tcttttgcca
     1921 cgttttttac gatatgtcaa aacagaaaca cgctccgatg caacaagcaa aacgactcca
     1981 tcgactcaga cacaagttgc ttttgcgcaa gaattgaaaa aagaattaga agagttaggc
     2041 ttagctgatg ttttctataa tgaagataac ggattcgtta tgggaacatt accaagcaat
     2101 gttgatcatg aagtgcgttc cattggattt atttcccaca tggatacagc agattttaat
     2161 gcagtaggag tcaatccaca aatcattgaa aactatgatg gcgagtctac gatcgtacta
     2221 gatgcggaag aacgctatac attgaataca aaagattttc caaatttgaa aaattatgaa
     2281 ggccacacat tgatcacaac agatggcagt acattactgg gagcagatga caaatcaggg
     2341 attgcggaaa tcatgactgc aatggagatc ttgatcaata atccttctat ccctcacggt
     2401 accatcaaag tggcttttgg tcctgatgaa gaaatcggtg tcggagcgga taaattcgat
     2461 gttgagcaat tcgatgtaga ttttgcttat acagtcgatg gtggtccttt aggtgagttg
     2521 cagtatgaaa cgtttagtgc tgctcaagca aatattacga tccaagggaa aaatgtacat
     2581 ccagggacgg caaaagatac aatgatcaat gctctacagt tagcaatcga ttttcataat
     2641 cagcttccag ccgatgaagt tccagaaaaa acggaaggat acgaaggttt cttccatttg
     2701 atggcattgg atgggaatcc tgaagaagct tctatgagtt atatcattcg cgatcatgat
     2761 cgtgaaaaat tcgaagcacg caaagcacaa atcacacata tccaagaaga gttgaatcag
     2821 cgatttgatc aagaacgaat caaagtagat atgtatgatc aatattacaa catgaaagaa
     2881 atcattgaaa aagatatgag catcgtggaa ttagcgaaac aagcaatgat cgagcttggt
     2941 atctctccag tgatcgaacc agttcgtgga ggaacagatg gttcaaaaat ctcttacatg
     3001 ggtattccga cgccaaatct atttgccgga ggagaaaata tgcacggacg attcgaattt
     3061 gtttctttac aagtaatgga aaaagcaaca gacgtgattg taaaaattgc agaattgaac
     3121 acaaagagtt aaaatcgaaa aagaacagct tatgacaagg cgttcgtccc aatgatggaa
     3181 tgtcatctgt ctaaggctaa agggcaggac aatggaattg gttgtcccgc cctctttttt
     3241 gaagagtaaa taggaggtaa agaatggaaa agctgctagt tttttataac aaaagttcag
     3301 ggaaagatga gggtgagaaa ttagcacgct ggtttcaaga ttatgtaaaa gaaaagcagc
     3361 cagaattata tgtagaacta gctgaaactg gcccatcgat tgatgatgcg gaattagtca
     3421 agaaagcaga agccatagaa gctgatactt tagtaattat aggtggagac ggcacgattc
     3481 atcatatcgt ccaagcgttc caaaaaacat tatctcaata ccatgtcgga ttacttccag
     3541 gtggaactgt caacaacctt gcacgtgttc ttgagattcc cctcaaaaaa gaagaagctg
     3601 ctgacgtgat cctggaaggc aatgtacgga aaatagacta tggtcaagtc aatgatgaag
     3661 tcattatctc tacactgaca atcggaatct tagcagatac agcagtttgg atcagtcaag
     3721 aagaaaaaca gcgttacggc tcgtggatct ttatcaagaa gttttttaag ttattgctga
     3781 agaagaaaaa atatcatttg gatttagaaa cagacgagga gcattggaaa ggaaaatccc
     3841 aactgttgac agtcacgatg tctaactccg tgggcggttt tacaaatttt gacgactcag
     3901 cgaccctaga tgatggaaaa tttcatgtaa cgatcgtacc ttccttagat attttccgat
     3961 ttatttttta tttgcctaga atcatgagag gcaaattcta tacgatcccc ggcattactt
     4021 atttcacagc caaacgcatg aaaatccaag cgcaggaaaa atctgtgggc actcgaacgg
     4081 atggagacac gacagatgat cttccagttg aattatctgt tgtatcaaaa ggattaacag
     4141 tttattcacc aaagaaagaa gaaaaagctt agaaatagta gttaacagaa agttggaaga
     4201 agagtaatgg atgtattgtt acacaatcct ttgtggtttt accttttcat tgtgaatttt
     4261 tacttatttt gtctgatggg gtatgataaa catcaggcta gaactagcgg ctggcgtgtt
     4321 cctgagaata atttgatatt tgtcagcctg ttaggcggtg gtttgggagg attgatctct
     4381 cgaaaagttt tccatcataa aactcggaaa aagaagttta ctataggatt cgctgcaggt
     4441 gtacttgttg atttgattct catttatttt ttccattaag gagcaggaga tgtcgtctaa
     4501 aggaacgaaa actaaaatca ttctgaatct gcttttgctg attgtgattt ttgccattat
     4561 ttattattta atcaatcaat catttgcaga tatctttgcc gaattgctgt ctaccagtgt
     4621 tgttgttttg gcagcggctg ttatattagg gacagtttac caaatcgctg aagggcgctc
     4681 aatcaaagaa attgctgaac ctttcgcgcc gaattttaca acgaaagatg ggttcttgac
     4741 ctctagctat attgcgtttt accgaatcgt ttcgtttggt acaggaacgc tgatctcgga
     4801 gatttatttt tataacaaaa aaggaatgaa gctatcccag ggagcagggg tgacagcact
     4861 ccatatgatt atgtacaaac tggcagtgat cacttatgca ttagctggct tggtcatcca
     4921 attctctctt ttttattcta atggtccatc aatgatctgg tttatcatcg ctggtatcct
     4981 tttgacgatc gtgattgttg cctttttatt ggcgatgtcc gtcagcttga atgttcaaat
     5041 attttttgtc agcttatcga ataagttatt taaaagtgag cgtttgcgga aatgggtgga
     5101 tacatgcaat cagcagatct actctttaag ggaaacggtc caaaccatta tccaagacag
     5161 gactgcgcta ttgcggattt atctatggaa tatgttaaaa cttgtatttt ggtatgtact
     5221 tccttatttg attctgattg aaaaccatcc taaaatagat tttctactga ccatttcgtt
     5281 tatcagcttt gcagtagtgc tttcaggggt tattcctacg cctgctggta ttggttcctt
     5341 tgaatttgtc tatttgttat tattcagacc agttgttgga acagttgacg cagtttcttc
     5401 gcttttactg tatcgtttca gttcctttat cttgccattt ctctatggat ttgtgtatgt
     5461 gattgctgaa cggcgaagag tgatcaaaga agaaataata gaagtaaaaa aagagaataa
     5521 agaaaatcaa gggtaggtct tttataagat ctaccctttt tatcaaatca aagaaaaacg
     5581 ttatggataa gataatttta cgtttgtttg gctctttttt tatatacata caatcaattt
     5641 tgttttttta agatttatat aagtcgagaa aaaaagtcaa aaaactatgt gtatattttg
     5701 accaaaattg accaaagcga tataatagtt tttgtaaaga tcaaagaaat aagtgatctt
     5761 acctttaatt aggagggtat acctgtatga atattgagaa aatgactacg acgcttcaag
     5821 aagcgattgc tgaagctcaa cagattgcgg tcactcgaaa acatcaagat atcgacattg
     5881 cccatgtatg gaaaatcttt ttacaatcta atcattttgg tcgaaatttc tatacggatg
     5941 ccggattgga tgtcgaatct tttgaacatg aaattgatcg tctactggat gagtatccag
     6001 ttgtttctgg tggaaacgtc caatatggac aaaacctcag ccagaatttc tttcgattat
     6061 tgaatgaagc agaccaaata cgtgaaagct tcggcgacga gtttctttca acggaagtcg
     6121 ttattttagc gttgatgaaa ttaaaaaatt atccgttaac tgtttatttg aataaaaacg
     6181 gattatctga aaaagaattg cgtaaaaaca tcgaagagat gagaggagga gaaagagtga
     6241 cctcacaaaa ccaagaagaa caatacaaag cattagaaaa atacggcgtt gatttagtac
     6301 agcaagtcaa aagcggaaaa atggatccaa tcatcggtcg tgatgaagaa atccgtgatg
     6361 tcatccgtat tctttcacga aaaacaaaaa ataatcctgt tctgatagga gaacccggtg
     6421 tagggaaaac ggcaatcgtt gaagggcttg ctcaacgtat cgtacgcaaa gatgtgcctg
     6481 aaaatttaaa agataaaacg attttttcat tagatatggg tgcattgatc gctggagcaa
     6541 aattccgtgg tgaatttgaa gaaagactaa aagcagtctt gaaagaagtc aaaaaaagtg
     6601 atggacgtat cctcttgttc atcgatgaaa tccacaatat cgtaggggca ggaaaaacag
     6661 aaggaagtat ggatgccgga aatctgttga aaccaatgct tgctcgcggt gaattacatt
     6721 tgatcggtgc cacaacttta gatgagtacc gtcaatatat ggaaaaagac aaagcgttgg
     6781 aacgtcgctt tcaaaaggta ttagtcaaag aaccaacagt ggaagatacg atcagtattc
     6841 tgcgtggatt aaaagaacgt tttgagatcc accatggtgt taacatccat gacaacgcat
     6901 tagtagcagc agctacttta tctgaccgtt atattactga tcggttctta ccggacaaag
     6961 caatcgattt gatcgatgaa gccagtgcaa cgattcgggt agaaatgaac tctatgccaa
     7021 cggaattaga tcaagttact agacgtttga tgcagttgga aatcgaagaa gctgcactga
     7081 aaaaagaatc agatgatgca agtaaaaaac gcttgaaaaa tttgcaagaa gaattagctg
     7141 aacttcgtga agaagcaaat gcgatgaaga tgcagtggga aacagaaaaa gaagaagtca
     7201 attctgtttc agcaaaacgt gctgaaatcg ataaagcaaa acacgaatta gaagatgcag
     7261 aaaataacta tgatttagaa cgcgctgctg tattgcgtca tggtacgata cctcaattag
     7321 aaaaagaatt gaaggaatta gaagctaaag caaaagacag cgagattaag atggttcaag
     7381 agtccgttac cgaaaatgag attgctcaag tcgttggcag attaacgggt atccccgtaa
     7441 cgaaacttgt agaaggtgaa cgagaaaaac taattaaatt gaatgaaaca cttcataaac
     7501 gagtgattgg tcaagatgaa gcagttgatg cggtcagtga cgcggtaatt cgttcacggg
     7561 ctggattgca agacccaaat cgtcctctag gttctttcct tttcctagga ccaactggtg
     7621 tcggaaaaac cgaattagcg aaagcgttag ctgagaactt gtttgactca gaagatcata
     7681 tggtacggat cgatatgagt gaatatatgg aaaaacatgc tgtgtctcgt ctagtcggag
     7741 ctcctccagg ctatgtaggt tacgaagaag gtggtcaatt aacggaagct gtcagacgaa
     7801 atccttatac aatcgtcttg ttggatgaaa tcgaaaaagc gcatcccgat gtcttcaata
     7861 tcttgttaca agtcctagat gacggacgct tgactgattc aaaagggcga gtagtagatt
     7921 tcaaaaatac ggttctgatc atgaccagca atatcggttc acagttactg ctggaaggcg
     7981 tgactgctga tggaacgatt ccagaagcag tggctgaaca agtcaacacg ctattacgtg
     8041 gaaacttcaa accagaattc ttgaaccgaa tcgacgacac gattttattt acaccattga
     8101 gcctagacaa cgtgaaagga atcgttgaca aaatggtggc gcaattggca caacgtctgg
     8161 aacatcaaga aatcctgctg acaatcagtg atgaagccaa aacgtggatt gctgaaaatg
     8221 cttatgaacc agcatatggt gcaagaccat tgaaacgatt tatcactaag gaagtagaaa
     8281 caccactagc taaagaaatc gttgcaggac atgttatgcc taaatcaaaa gtaacgatta
     8341 ctttacttga tggacaactt catttcaaaa cagaagagct agaagaaatc gtctaatctt
     8401 ccattgagac tataaaagaa aaaaggggtc tgacacaact agtgtcagac ctcctttttt
     8461 caaataaaca gtgttccaga agtagtttct tcgaaaaata agccgaaatt tcttgaagct
     8521 gcccgcttct gtcacaacct cttttattag gtatttttca ctttttctaa tttataactt
     8581 tcaaagtaac gctcatcttt tttactttga gaccatgcaa acaatcccat taaggcagca
     8641 cctatcgtat ttgtcagcaa gtcgcccatc gtatccatca atgctgctcg tccgacaaac
     8701 agtgtgccat cagaagcagc aaaacgttga agattcatgc ctaagaactg gtcgcataag
     8761 aattcccaga actcccaaaa cacgccgcaa agtccagcaa acgcaaagcc gaataagagg
     8821 aacaaccaag gactggtttt tgatatctca gcatctttca tcaagtaacc aatcaatcca
     8881 tagccaagag ctgtcaggac cattggactc accgcatgta agattttgtc ccaaaaagag
     8941 atgatactga tcaaatgcat ccctgtgcct aaaaagacag agatgaacaa gaaaaaccag
     9001 taaaacaaaa cgattgctgc tggtatttta agtttgaaaa ctttcaaaat gacttgtggc
     9061 atgaaaatca atgcaatccc taataaagat tccacgataa atgtgattcc ttgagaagtc
     9121 gaatattttt gtgtgataaa ttcataaaaa ttatagcaga caacgaatac accaaaagca
     9181 agtaaagcta tttgcacctt tttgatttct ttaatcactg aaagtccctc catctaaaaa
     9241 tctttttact tctgaaagca actcaatttc tgaagaaacc acttttccgt tcaatttgat
     9301 caaacctatt gtatataagt tcaggtagtg aaattgattt tcagctgttt cttgtagtgc
     9361 agcgattttt ttcgggtggg ttccgccttg ctgtcgagta tcagtatata agccgatcac
     9421 tgggatgcct ttcgcataag ccacaccgat ttccgatgct accccggcat cgatcgtcag
     9481 cccatctagt aaagcaatca ttaaatcact agccaacact ttttctgtgt cagcaagagc
     9541 aatcattcga ctgtctgcat aagcagtttt gtcattgatt gcttcatttt cttgaggaag
     9601 atagatcgaa agctcagaag agagctctct gatttcttta actaactgtt ggttatataa
     9661 caaatcactt ttagcaaaca ttggtgcggc aaaataaatt ttcatctaaa catcatcctt
     9721 tttatcaaaa ataatcgcta aagtaagctt ccatgtttgg aatgatacgc tctagtgact
     9781 tcagtgtttc ttcatctgtt tctaaccgtt cgattcttgc caaataagaa gcacggcgat
     9841 aattcatcat caagcaagtc aatgtttgga tatccagtcg aacggatttt cctataggtt
     9901 catttaagac acggacttgt ccctgttcat cccatgttaa agcgaagaca ccattattcc
     9961 aaggagcgac aggatcttca atgatgaaat gaaaagcatc agctgttcca acaaacggaa
    10021 accgttgaag gaattctttt acatcaacga tccgagccat aaaatatggt tcgatctgct
    10081 ctttgatctg actatcttca atcaaaaatg ctaaaggctc atttttataa atatctccat
    10141 gtacccagta gatcatggaa aaatgtgcgg aaataaagtt ccataagcca tttcgagctt
    10201 cttgatcaag atagaacatt tcttttacgt gaaatatttc ttcagctacc cagtagaaca
    10261 gaacacctct tggttcatgg ttgctatcgt agtagacagc ggctgtgcgc tcatcttcat
    10321 tttcgaaacg ccaatactct tcccaatgaa aatcactgcg gaataaagca ccatggtttt
    10381 gttgggcaaa tttgtcataa acggaaaata cgtctggatg atcaactggt aatcgctcaa
    10441 ccataccact tactgggatt tgtttaggta gctgtgtatc tctgatttta aaggaaagtt
    10501 tatcagacat gatttcccat cctttacgcc gatagtaggg gatactgtaa ggatacaaat
    10561 aagagatcca ttgcttattc ttgcgcatat tatccaatgc aacgataatt agatcttgca
    10621 tcaatccatg gttagcatat tctggataag ttccaactcc agtcacacca cccattttat
    10681 aacgtgcacc gtgaatattg acttcacaag gatagatagc gatttgagag atcagtttat
    10741 tttcgtggaa ccatccgaac actttcgata cttccaaaat cggttttttg gaacggataa
    10801 aagctcgttt attctcaaac ccgctttcct cgatgtctgc ttcagtcact tggaacacat
    10861 aggaaagcaa ttcattgaat tgatcgatat gttcttgagt gaccggtttt aattcaagat
    10921 tttctctaaa atctttttga tccatttttt catctccatt tattagcttg ataacagtat
    10981 agcaaaattc tacaaaaaaa acttctatag ctgactaatt aaaaactctt taaattactg
    11041 cttttcgttg gtataatgga aagtgctgat tatatgaaaa gaggagacgc tatggatata
    11101 aagaaaatga aagaacgcca agagcagatt cgtaattttt cgatcatcgc tcatatcgac
    11161 catgggaaat ctacattggc cgatcgtatt ttagaaatga cgcacactgt gacgtctcgc
    11221 gaaatgcaag atcagctgtt agactcgatg gatttggaac gtgaacgtgg aatcacgatt
    11281 aaattaaatg ctgtagaact caattacaca gcgaaaaatg gagaaacata tatctttcac
    11341 ttgattgaca ccccagggca cgtcgacttc acctatgaag tctctcgaag ccttgctgcc
    11401 tgtgagggtg ccgttttagt ggtcgatgca gcacaaggaa tcgaagcaca aacattggcc
    11461 aatgtttatt tagcgttaga caatgatttg gagatcctgc cagtcatcaa taaaatcgac
    11521 ctacccgcag cagatcccga acgtgtccgt caagaaatcg aagacgtaat cggaatcgat
    11581 gctagtgaag ctgtattagc cagtgccaaa tcagggattg ggattgaaga tattctcgag
    11641 cagatcgtgg aatatgttcc tgcaccaagt ggagacttag atgcaccgct aaaagctttg
    11701 atctttgact ctatttatga tagttaccgc ggtgttgttc taaatgtccg gatcacggac
    11761 ggtatggtca aacccggaga caaaatcaaa ctgatgagta atggcaaaac atttgatgtt
    11821 acagaggtcg gggtcttctc gccaaaagca gttgctagag actttttgat ggtcggtgat
    11881 gtcggttata tcacagcaag tatcaaaact gtccaagata cacgagtagg ggatacagta
    11941 acgttagcag acaatcctgc cgctgaagct ttaccaggtt accgaaaaat gaatccgatg
    12001 gtttattgtg gtctttatcc aattgatact tctcgttaca acgatttgcg ggaagcttta
    12061 gaaaaactgc aattgaatga tgctgcttta caattcgaac cagaaacctc tcaagcatta
    12121 ggttttgggt tccgctgtgg attcctcgga cttcttcata tggatgtcgt ccaagaacgc
    12181 ttggaacggg aattcaatct agaattgatc acgactgcac catccgttat ttaccatgtt
    12241 aataaaacgg atggtacgac tgctacagta gataacccgg cagattttcc tgaacctgta
    12301 acgattcagg atgtagaaga accattcgtc aaagcacaga tcatggtccc aaatgatttt
    12361 gtcggtgcag taatggaact ttctcaacga aaacggggag aattcattac gatggattat
    12421 ttagatgatt accgagtaaa tgtcgtctat aatattccgc tttctgaaat tgtctttgac
    12481 tttttcgaca aattgaaatc aagcacgaaa ggctatgcct cattggatta tgaaatgtct
    12541 ggttatcaaa aaagcaagtt agtgaagatg gacatcttgc tgaatggaga aaaagtcgat
    12601 gcattaagct ttatcgttca ccgagacttt gcctatgaac gtggaaaagc aatcgttgaa
    12661 aaactgaaaa aactaatccc acgccaacaa tttgaggtgc caatccaagc agctattgga
    12721 caaaaaatcg ttgcccgttc agacatcaaa gccttgcgta aaaacgtctt ggctaaatgt
    12781 tatggtggag atgtttctcg taaacgtaaa ctcttagaga aacaaaaaga agggaagaag
    12841 cggatgaaac aaatcggctc cgtcgaagtt ccgcaagaag ccttcatggc cgttctgaaa
    12901 atggatgagc aagatagtgt gaaataactt ataatcaatc gaaaaaccta ttaaataaac
    12961 ctttttgagt agatgcaaca ctgaggatta tctgtataaa agattttaaa ctgtcatttg
    13021 gttatcacgg cgatactaaa attaacaaaa ttggaatatt tttcttgaag atttttagct
    13081 gtcagtatat gatgaaagca tctcatagga ggtgcttttt tcatggttga taattcaagt
    13141 ggaagggttc ttaaatctct aagaaaagag aaaaaactat ctcaaaaaaa gttagctgat
    13201 ttagcaggta tttcacaaag tactttagtt aaatatgaaa aagggtcaag aaaaatccct
    13261 aaagacgtag ataatactct aagcaagatt ctgaatatag aaactctaat aaaagatgag
    13321 gaagataaga t
//
LOCUS       DANKKG010000071        12915 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000071 DANKKG010000000
VERSION     DANKKG010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12915
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.071"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(28..963)
                     /gene="murB"
                     /locus_tag="PW004_001715"
     CDS             complement(28..963)
                     /gene="murB"
                     /locus_tag="PW004_001715"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HDL2726688.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1036..1956)
                     /locus_tag="PW004_001716"
     CDS             complement(1036..1956)
                     /locus_tag="PW004_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL2726689.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2069..2821
                     /gene="xth"
                     /locus_tag="PW004_001717"
     CDS             2069..2821
                     /gene="xth"
                     /locus_tag="PW004_001717"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HDL2726690.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            3685..4218
                     /locus_tag="PW004_001718"
     CDS             3685..4218
                     /locus_tag="PW004_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HDL2726691.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(4708..5232)
                     /locus_tag="PW004_001719"
     CDS             complement(4708..5232)
                     /locus_tag="PW004_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2726692.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(5225..5698)
                     /gene="tsaE"
                     /locus_tag="PW004_001720"
     CDS             complement(5225..5698)
                     /gene="tsaE"
                     /locus_tag="PW004_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HDL2726693.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(5821..6801)
                     /gene="pta"
                     /locus_tag="PW004_001721"
     CDS             complement(5821..6801)
                     /gene="pta"
                     /locus_tag="PW004_001721"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HDL2726694.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(6954..7634)
                     /locus_tag="PW004_001722"
     CDS             complement(6954..7634)
                     /locus_tag="PW004_001722"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDL2726695.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            7760..8629
                     /locus_tag="PW004_001723"
     CDS             7760..8629
                     /locus_tag="PW004_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL2726696.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            8655..9170
                     /locus_tag="PW004_001724"
     CDS             8655..9170
                     /locus_tag="PW004_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726697.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(9217..9810)
                     /locus_tag="PW004_001725"
     CDS             complement(9217..9810)
                     /locus_tag="PW004_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2726698.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(9835..10014)
                     /locus_tag="PW004_001726"
     CDS             complement(9835..10014)
                     /locus_tag="PW004_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726699.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(10091..10600)
                     /locus_tag="PW004_001727"
     CDS             complement(10091..10600)
                     /locus_tag="PW004_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726700.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(10696..11076)
                     /locus_tag="PW004_001728"
     CDS             complement(10696..11076)
                     /locus_tag="PW004_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL2726701.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            11406..12155
                     /locus_tag="PW004_001729"
     CDS             11406..12155
                     /locus_tag="PW004_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HDL2726702.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(12476..>12915)
                     /locus_tag="PW004_001730"
     CDS             complement(12476..>12915)
                     /locus_tag="PW004_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HDL2726703.1"
                     /translation="KKAEGSPCFLLYQTFPGIGALTAALLLGELGDITRFKTHKQLNA
                     FVGIDIRRYHSGKYTGQDRINKRGNPKARKIIFFTIRNMIRQQRAAPNHIVDYYYKLK
                     KQPIPKKDKVATVACMNKLLKCMHAMVRAHTEYDYAYAVSVDH"
BASE COUNT         3733 a         2612 c         2321 g         4249 t
ORIGIN      
        1 tttacttatt ttaagttcca ttacttatta attggctaaa cttctgcttg tttctgatat
       61 tgcttgtttt tctccaatga tccgcacttc tgtttctaaa gacacatcaa atttttcttt
      121 gattacttct tggatgtgcg caatcaattc aatataatct gtcgctgtcg catgatcgat
      181 attgacgatg aatccagcat gtttttcaga gacttgtgca ccgccccatt tcaggccttg
      241 caatcctgct tgttggatca gctgacctgt atagtgtcct actgggcgct taaagacact
      301 tccgcatgaa gggtattcta atggctgttt tgattgacgc aattcggtca actcatccat
      361 tcttgctttg attgcttcat aatcccctgt ttgcaaactg aatcgagctt ccaatacgat
      421 agcacgtaat tcttggactt tactgtgacg ataagaaaat accatttcgt cttttgtcaa
      481 agttttcaat gttccatctt caagcaaaag atccacttca gcaaaaacat ctttgatctc
      541 tccgccatac gcacctgcat tcatataaac cgctccaccg acacttcccg ggatgccgca
      601 agcaaattca aatccagtca atgattctgc caaagctgca taagtcgtat caatcagtga
      661 ggcacctgct tccgcaatga ccatcgttcc tttgacatga atctgtttca tttcagtcag
      721 catgatgact acaccgcgaa tcccgccatc ttgaacgatc agattgcttg catttcctaa
      781 taccatccaa ggtatatcat ggattcggca ataatcgatg atttgcttta cttcttcttg
      841 ttttttcggg aatgcaagga catcagctgg tccacctgtt ttcgtaaacg tatagttcat
      901 caatggttca tcaaacaata tagtaatcgt ggctaagctt tcaactattt cttctttatt
      961 cactgaggat tttcctttct tttctcaaac gtcctcatta ttctatcacg cttaatacaa
     1021 tcttgccagt cttgttcaaa gttgatgacg gtagtgatct ttaagcactt cctcacataa
     1081 ttcatgactt cttacaactt ctttttgttt aagcaattct gtttccgatt tacctaaacg
     1141 gatctgagaa aagaaggcct ctaccattgg ctcaaacccg cgtttgacta gcgtgtttgt
     1201 ccagtcgcca aattctttca ctttttttcc atcagcatct tgaatgacaa gctgggtcag
     1261 atcgtttact tcataagtac catttttact tgttacttga tatttttcta tattagcgcc
     1321 acttgctaaa tccatcgtta aaatagccgt ttgattttgt gtttctaatt gtaaaatggc
     1381 atattccaaa aattcctttg tttctcttat ctggtatttt gttctcaaaa tgggttcatc
     1441 taataaatac acagctgtat caataagatg taaaaacaaa tcataaagga cgaactctgt
     1501 cgtttctttt gccgctattc gatttttttg caggtggatc aaccgcttat ctgggatacg
     1561 tttcaattct tctaccatag gagcaaagcg ccggttgaaa ccgatcatca aaattatttg
     1621 gttcttatca gctaattctt gcaattcacg aacttcattt agatggacac tcagtggttt
     1681 gtcaatataa acatggatac cattttctag acattttttg gctaattcat aatgtacttt
     1741 tgttgctgcg tggatcatac atgcttcgat tttttcttct aaaagctcat cgatcgtttc
     1801 atatagatgt tggaacccgt actctttttg aattctttct tttgtttctt tatttcttgt
     1861 tgcaaaataa aaatcaagtg ttccttgttt tttcgcataa gttggtaaat atgctttttc
     1921 tgcgatattc cctacaccga ttacaccaat cttcatctac tttcctcctg gatcatttag
     1981 ttactttcat cttacaccat tttttactaa aatgaggtac aatagtatcg caaaggtaag
     2041 gaaattaatt cgaaggagtg tgcttttatt gaaatttatc tcttggaacg taaacgggct
     2101 tcgtgccatc gtaaataaaa attttttaga agtttttcat gagttagacg ccgatttttt
     2161 ctgtctgcag gaaacgaaac tccaagcagg acaaattgat ttagatcttc caggttatta
     2221 tcaatattgg aattacgcgg aacgaaaagg ctattccggc acagcgattt ttgctaaaaa
     2281 accagcattg aatgccactt acggaatggg tatcgatatc catgatacag aaggccgtct
     2341 gattactttg gaatattccg atttctttct tgtaacctgt tacacgccaa attctcaaag
     2401 cgaattaaaa cgtttagatt accgattaga atgggaagaa gccttttata attatttgga
     2461 aaatctaaaa aaacaaaaac cagtcatcgt gtgtggggat ctaaatgtcg ctcatcaaaa
     2521 gattgatttg aaaaactgga aaacgaatca aaaaaatgct ggattcactc cagaagaaag
     2581 agcggctctt tctcgactgt tggataacgg gtttatcgat acattccgtt atttttatcc
     2641 aacccaggaa ggtgtctact cttggtggaa ctaccgattc aattcaagga aaaacaatgc
     2701 tggttggcgg atcgattact ttttgacgag caaagattta gagccaggac tagctgatgc
     2761 aaagatccat acggacatta tgggcagtga tcattgtcca gtagaattag atttgaaata
     2821 aagaggttgt gaaaaagtcg tttagtttcg agtattaaga aaaattttgt gaaaatggct
     2881 ttccacattt tgcataaatt tggcataata ccgagaaact ggcttttgaa cactgtttat
     2941 cgagagattg tgaaaaagct gtcctgcatc aagtaataag aacagccaaa caaaaatagt
     3001 tcctcatatt tttcgtttgg ttgggcttat taccgcagat acaagctttt gaacaccgtt
     3061 tattaagagg ttgtgaaaaa gctgcactgc atcaagtaat aagaacagcc aaacaaaaat
     3121 agttcctcat atttttcgtt tggttgggct tattaccgca gatgcaagct tttgaacacc
     3181 gtttattaag aggttgtgaa aaagctgcac tgcatcaagt aataagaaca gccaaacaaa
     3241 aatagttcct catatttttc gtttggttgg acttattacc gcagatgcaa gcttttgaac
     3301 accgtttatt gagaagttgt gaaatcagcg tcctgacctg agtattagag aaacaaatgg
     3361 caaaatagtt cctcatattt tgctattttt tgagctaata ccgagggtca gctgattgaa
     3421 caccgtttag tggagaagtt gtgaaaaagt cgcctacttt cgagtcgtaa gaaatttttt
     3481 tgaaaatggt tcctcacaac tttgcataaa tttgacttaa taccgagaag tcatgatccc
     3541 catctaactc caaatcacaa ggaatgagtt caaaaactag cttttctcaa caaatattac
     3601 ataaaaaatt ctatgttttt tgttaaattt atttgctttt ttcaaaagta tgttacagta
     3661 atttcccaag gaggttttac agaaatgaat tttgttgcca tggattttga aactgccaat
     3721 catcagcctt atagtgcttg ttctcttgcg ctagtcatgg taaaaaatag ccagatcgtt
     3781 gatgaattct atacattgat acagcctgag actccttttt tctggcgaaa tgtccaaatt
     3841 cacggaatcc atcaagaaga cgtacgcaat gcgccgaaat tcccagaagt ctggcaaact
     3901 atccaaccat attttcaaaa aaatcgttta gtcgttgcac ataatgctgc ttttgataca
     3961 aaggtcttag cgggatgctt agattattat catcttgaac agcctaatta tctttcttta
     4021 tgtacagtga aaagtagccg tcgcctgttt cctgaatttc caaatcatcg attgaacact
     4081 gtgtgcgaaa atttgaacat ccaattagat catcaccatg atgcattgga agatagtcgt
     4141 gcatgtgcgg aaatcctgct ttatcaagaa aaaaaattcg ggacagatcc attgaaaaaa
     4201 cttgtgacta taaagtaaaa gaatacttat gaaacacaca aagaggtcgt gagcctgccg
     4261 tttcactccg agtcacaagg agcaatttca aaaaatagct tctcatgttt attaaatatt
     4321 gtgactggtg accgaggagc tggcaggaga acaccgttta ttggagaggt tgtgaaaaag
     4381 ctgttctgca tcaagtaata agaacagcca aacaaaaata gcttctcata tttttcattt
     4441 ggttggactt attaccgcag atgcaagctt ttggacaccg tttaccaaga ggttgtgaaa
     4501 tcagcgtttt gacctgagta ttagagaaac aaacggcaaa atagttcctc atattttgcc
     4561 gttttttgag ctaataccga aggtcagctg attgaacacc gtttattcaa aaaaaatgga
     4621 ctgcgacacg acttttgtca cagtcccttc gtgtgttttt ctgccttctc aaaaggatga
     4681 cagtttctca ctcttttcct atttcttcta ttcaatcaaa taactcattt tatatacatc
     4741 taaaaattca cctagatctg ttcttgcccc tctaggcatg accgcttcga tttggaagcc
     4801 catttttcgg taaaggtgta cggctctttc attacgaact tgaacgtcaa gctccaatcg
     4861 aaaaagtaca cccatttcct tcgcccaact gatgatttct tctagcataa gagtgcctag
     4921 tcccattccc caatattcct gcagaatact gataccgact tctcccacgt gagacaaacg
     4981 aaattgactg tctgccttga cagatgctgt tccaataatc gtcccttcat agattgcaag
     5041 cagcaataaa ttattattgc tttcgtatat ataatccaac tcttcttcta aggtttctgg
     5101 tggtaacagg agttctgctt catccaaaac taaaaattct gtctccttat taacttgtcc
     5161 catcatcaaa agaatgtctt ttgcatctcc tggcactgct tcccgcaaga tgatcccaac
     5221 ttcttcactc atgattttca gcccattttt ctagaattcg ctgtttgaag gtttcacttt
     5281 gctccccgta tgagattagt ttcacatacc gcttttctaa atctgtatca cttttttcta
     5341 aaatcagttc caaataatcc tccggcagtg cttcttctaa tagatttccc cattcaacaa
     5401 cggaaagccc atcaccttca aaatattcat ctaaccctaa atctgctccg ctagctgcta
     5461 cccgataaat atccatatga tacaacggaa tccgaccttg actatattct ctaataatcg
     5521 tgtatgtcgg acttttgatc atttgcgtta tccctaatcc ttgggcaatt cctttcgtca
     5581 acgttgtttt acccgcacct agatcaccag tcaatatcag attgtcacct ggcattgcgg
     5641 aatgaccaat tgcctctgcg aattgttctg tcattgttaa tcctgatagt tcgatcattt
     5701 ttcatcttct cccgctcttc tttctttttg ccagtatatc gcgtaatgaa gtttttttaa
     5761 agaaaaaatg aactagtcaa gaacagcagc tgctgttttt ggctagttcg cgtgtatttg
     5821 ttaatcaatc aatgtttgtg ctgctgtgat aatcgataat ttatatacat cttcttcatt
     5881 tgctccacga gacaaatcag aaaccggttt gttcaagcct tgtagaattg gtccgattgc
     5941 ttcaaagtta ccgaaacgct gagcaatttt atagccgata tttcctgact gcaattctgg
     6001 aaatacaaag actgttgctt gaccagctac ttctgaatct ggcgctttca gttgtgcaac
     6061 tgaaggaaca taagaagcat caaattgcaa ttcgccgtcg atcatgtatt gtggcgctag
     6121 ttttttagcg attttagttg cttcaactac tttgtctact tctggcgatt tcgctgagcc
     6181 ttttgtcgag aagctcatca ttgctacttt tggttcgata tcgaacaatt ctgctgtttt
     6241 tgcagaatct acggcgattt cagccaattc ttgcgcagtt ggattcacgt tgatcgcaca
     6301 atccgaaaag acatattttt cttgatctct tccacgtacc atgatcatcg ctccgcttgt
     6361 ccgactgatg ccaggctttg ttttgatgat ctgaagtgct gggcgaacag tgtcgccagt
     6421 cgaatggatc gctccagaga ccattccgtc tgcaatcccc atatacgtaa gcatcgtacc
     6481 aaagtaattc acatctttta aaatcttttc tgcttgttct ttggatgcct ttccatttct
     6541 acgttcaaca aaagtatcca ccatttcatc ccacttatcg taattatcag ggctgatgat
     6601 cgtaaagcta gatgttttga ttcctctggc atgcgctgca tttcttattt cttcttcgtt
     6661 ccctactaaa atgggttcca caagttcttc tgctttcaac cgggcagctg cgcctaaaat
     6721 acgagcatca gtagcttccg ggaaaacgat tttgatccca cggcgaacaa ttttaaatct
     6781 taaactatca aacaattcca caagatgacc tccagacgtt tttatatgta ttatcataca
     6841 ctattccaga aaaaaagaac agtttatttt atctgtatca gtcaaaaatg ctggaaaaat
     6901 taagtttttc acatacaaag cgaactgcac tataacagga aagcgctgtt ttattacact
     6961 gtttcaggca gttgccaatc aatcggttct tctcctaacg caaggagcgc atcattcgtt
     7021 ttcgagaagg gtttggaacc aaaaaagcca cgacttgcag ataacggact gggatgtgga
     7081 gatgttatga tgatatggcg actcttatcg atcattttga ttttttcttg tgccggtttt
     7141 ccccacaaga taaacacgat tggtttttcc cgttcattta atttttcaat gatcgtgtct
     7201 gttaatcttt cccaaccttt cccgcgatga gaatacgctt gcccttcccg aacggtaaga
     7261 acggtgttga gtaaaaggac tccctgttta gcccatgaca ctagatttcc atgttgaaca
     7321 ggtgcgattc caagatcatc ataaagctct ttatagatat ttcttaacga tggcgggatc
     7381 tttacgccag gttgcacaga aaaagataag ccatgagctt gattttctcc atgataagga
     7441 tcttgtccta aaatcaccac tttaacttct tcataaggag tcaattccaa tgcttcatag
     7501 atatgataca tatctggatg gatcttttgg gacgcatatt ctttttttaa aaattctctt
     7561 agatgcagat aataatcttt tgaaaattct gaggacaaaa cttcttgcca actgttatga
     7621 atgatttctt tcaaaaactc cacctcctaa taaaaagaat aactcacatt ttcttcaatc
     7681 acaagggaca atcttcaccc tcagcacttg attaatatgg taaactagga acaagaagca
     7741 gaaaggaacg ataatcatta tgatcaaatt aattgcatca gatatggacg gcacattatt
     7801 agatgcccat atgagtgttt ctcctgaaaa tgccgaggcg atacgttttg caaatgatgc
     7861 cggtgttgag ttcatggtcg ctactggtcg aaactatcaa gaagctcgcg cagctttaga
     7921 tgaggtagga atcgattgtg cgatgatcac tttgaacggg gcacaggtat ttgataaaga
     7981 aggaaattca ttgtttaccg tgtcgattcc aaataatcaa gcaatctctg ttttagatat
     8041 tctagatgct aacggtattt actatgaagt agcaaccaac gatggtcttt attctgaaaa
     8101 tcaagcgaaa cgaattgaaa gttttgcttc catggtggct acccatttac ctcaccttac
     8161 gtataaaatg gcgattgcca tggcctcagc taagctggag ttattgcaca tcacatacgt
     8221 ggacagtatt cgtgctattt tggaagaaaa gaatctggaa gttttaaaaa ttatttgttt
     8281 tcatacagaa gggccagctg tattaggtcc agttggaaag cagatttctc ttttaggcga
     8341 tctagccgtt acttcctctg gtcaaaacaa tatcgaagtg aaccataaaa acgctcaaaa
     8401 aggaattgct gttgcccatg tcgctcacga gcgtgggatt tctttagaag aagtcatgac
     8461 gattggcgat aacttcaatg atgtcagcat gctgcaagta gcaggagtca gtttcgcaat
     8521 gggaaatgca gaaatcgaag taaaagatta tgccaaatat acaacggaca caaaccttga
     8581 atctggcgta ggaaaagcca tattgcgcgc gattgcagaa gacttgtagt tttatctaga
     8641 aaagagggat atagatgtca gaatttttca ttcaggaaaa gcagcttgga cgtgcaactc
     8701 ggacaattgt caaaaacgaa aaaggacatt ccctgttttt gatggttggt cgctggggaa
     8761 caaagggcga tgcattgtct ctttatgcca tgaatggcga tctcgttgct cacatcaagc
     8821 agataagcct gacattcggt acgcgttttg agctgtataa agagttcgaa aaggtgggaa
     8881 ccatgcagaa aatattcaat tggcccggtg acttttatta tattcgtaga cttcactgga
     8941 cagtccaagg tgatatctat aatcatcaat atcaaatcca tcatttcaat cagcaaatca
     9001 tgaaaatgga taaagcaaca cttttttcag gagattacta tgtattggat gtctcaaatg
     9061 atgaagatgc tccagtttgt atttgtattg ctgcggtttt agattactgg ctctaccgta
     9121 aagacaaaag tcgcgataag cctgtttctt accgacttaa cttaagttaa aaagatgtac
     9181 ctgaaatgcg gaaatacgca tttcaggtac atctttttag atattctttt gcatatgatc
     9241 ataaagatga ccactgatca tccgctgacc gacaactttg aagtcatggc gttcgtataa
     9301 ttttttagcc tttggatttt tttcatccac acttagaccg atgacggagc gattagcttt
     9361 cttcgccaat ttaggtaatg cttctaataa tcgcgaaccg atgccttgtc ctctaaaatc
     9421 ttctgataca ctgatagagt ccaaatacca ttcgttggga aacgtttctt tatcaatgaa
     9481 caaacgtgta gacgcatcta gctgatattt ctctaagata ggtaccagtg gctgatcgat
     9541 gattgattct tcttcatctg tataaccgta agcaactcct gcaactttac cgtctatttc
     9601 ttctacgata cctcgtgaat aactgtaacg ataatttgga tcagtgattg cttcttctaa
     9661 aatctctaaa gtcttttgtt cccctgtctg cttcaaaaat ggcagttcca tatcttttag
     9721 aatcactaag atcaatgggg cgatttctgg agcatcttct tttctcgcaa aacgaatcat
     9781 agtttcctct ccttttctcc attctaacat gcagaaaaag aaatcgcaaa atcgttattt
     9841 taaaaaatct tcaatctttt cagtaatgaa ataaccatga gtaacgattc ttgccgcttc
     9901 agttcggcaa taagggcaga cgactggcat ttctttatca ttatgttcga ctacattatt
     9961 gacttcttcg tatgcaaatt tttttccgca attagggcat ttcatttttt ccatgttatc
    10021 tcaacttcct ttcatcttta ctctcttagc ttactcttat attgtaaaaa agagaagcaa
    10081 tatccttctt ctatgcagat atccatacct gacggtcctg ttctccatct attaatcgga
    10141 ctttataata aggacgttca tctttgacta gaagtgtcca ctctttcact tttgcttttt
    10201 tcccgtagta gctgactgct tccttcatcg ctgcttgcgg ttgtttgaac tcttccgttt
    10261 tttctatatc aaaaagatgt tctgcggcct tttccgtgtc atttcgtacg gttgttttcc
    10321 cagtggatgg attgactgct atgacttgtc cttcacaaaa aaactggtat tcatatgttg
    10381 cattcgtagg attctccaaa tctttttcaa ctgcaaaatg aatagcagat atgttatgtt
    10441 cttttgtttc gttttttacg attgctgctg cttttttcaa tgagatccga tattcctggt
    10501 cactaatatc atccatgtga aaagcggcat tgagcagtcc cgtcataccg gtaatcccta
    10561 ttacgatgag ggcagcaaca ccaaacatca tttttttcat tcttcattct cctgtttctt
    10621 ttttttaaag aatatcaaga aatgaacgaa tgaaaaatcg aatgaaacga aaaaaaagtg
    10681 acaattttgt tatctttatt cgtgcagttc taatggcaat ccgtctggat caaagaaaaa
    10741 cgtcattttt tttcctgtaa aggtatcttc tctaatcggt tctgattcga tccctaagga
    10801 ctgcaattct gcaattgttt cttcaatatc gtctactttg aaagcaagat gacgcaagcc
    10861 acaagcttct ggatagtttg gtcgttgtgg cgcattagca attccaaaaa tctccaattc
    10921 agcacttcct agtttcagat ctaatttata gtcatctcgt tctttgcggt aattttctct
    10981 gattacttca aatcctaatt tattaacata aaaatcacgt gatttctgat aatctgaaac
    11041 aatgatggct atatgatgga tcgttgtaag gttcatatga gcactcctat tcttcgttta
    11101 agctcggatc tatattaatc tccagttcca ttgtaaatgt gtcagattga aaaaaatagc
    11161 tgtttttcct ttttataaga aaaaaggcaa aaaaaacaga aagtaggtcc tccaagacac
    11221 tcttcctgtt cccaatataa taaatgaaag taagaaatta tattttcaag aaaacaaaca
    11281 gaaaattatc ataacacaaa ttatttataa ttgaaagact agtttttttt caagaaaagc
    11341 atacaataga ttgtgttagt aaatacagaa cacactacat ttagtgtctt aaggagtcct
    11401 catacatgaa gtcatatttt cagtttttag ctcatcagct aaaaggctgg ccgcaacaaa
    11461 attattatct tttctttttc agtcttggct gccaggtcat gactcttgtc aatagtccca
    11521 tcacttggct gtccgttatt acatttatcg gtaccacgct tggtgtattg tgtattttat
    11581 ccatcaatgc ggcaaaatca gtcaacggta ttttaggtat tttgtctgcg atttgtttta
    11641 ttatcgttgg gttttcagca aagaactatt taagtatcgg tgaacagatt gcttatatga
    11701 tcacattgga tatccccgta ttgttaagta aagaatggaa tcataatatg gcttcaaaaa
    11761 tccgaaaatt cactggaaaa agctggttta tcgcaatcat cagtactttc cttgtctatc
    11821 ttgtttcagg attcctgatt ggtcgttata cagatgatcc tcggccgtgg gtagatgcca
    11881 ttgctttttc tattagcttg actgctggta tcatcagttt cttgcgttac aacaaccaat
    11941 attactggtg gttagcttca gggcttgctc aaatggtcct ttggtatatt tcatttacgc
    12001 atggatctgc aagtttggcg atgttcgtga acagctctgt ttacttgctg aatgatgtcc
    12061 ttgcatttac cgtttcacct tggtataacc aaaaagaacg tgaacgtatg caaaaaatgg
    12121 aagaagaata ctacaacgca caacaacacg cataaaaaaa ctgagcgtgg gacaaaagta
    12181 aagaacactt ttgtctcacg ctctaaacac gtataaacgg cggaatcaga agcaactcct
    12241 tcggaaataa gctgaaattc acaaaaattt gaaaagcaat tttcgtgaat ttcttcttat
    12301 ttcttggagt taaacgcttc tgtcccagcc tctatttcct acgattagtc aagtaaaagg
    12361 tgcaagaaaa atagctcgta aacgaaagcg acaaaatcaa agcacaccaa ataaagcccc
    12421 gttcgtaaaa aattttcgat ctgggcaaag aagtggtata ctgtaagtgg tttagttaat
    12481 ggtccactga gaccgcgtac gcatagtcgt actctgtatg tgccctaacc atagcgtgca
    12541 tacatttcag aagcttgttc atacaagcaa ctgtggcaac cttatctttc ttagggatag
    12601 gttgcttttt taatttgtag taataatcga caatatgatt aggagctgcg cgttgctggc
    12661 gaatcatatt tcgaatggtg aagaaaataa ttttccgtgc tttgggatta ccacgcttat
    12721 tgatgcggtc ttggccagta tacttacctg aatgataccg tcggatgtcg attccgacaa
    12781 aggcattcaa ttgtttatgg gttttaaatc gtgtgatatc tcctaattca ccgagcaata
    12841 aagcggctgt gagtgcgcca atcccaggga acgtttggta caacaaaaag caaggagagc
    12901 cttcggcttt tttga
//
LOCUS       DANKKG010000072        12864 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000072 DANKKG010000000
VERSION     DANKKG010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12864
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.072"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..2137
                     /gene="acm"
                     /locus_tag="PW004_001731"
     CDS             <1..2137
                     /gene="acm"
                     /locus_tag="PW004_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HDL2726704.1"
                     /translation="MLFIITNITSLIPVHVYADAGRDISSNVTSLTVDPTNITDGGNI
                     KVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVGTLTVRKDSAQ
                     VVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATVNVTKPASGSS
                     SSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRLDPSTFEINQI
                     HLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRKIMVSYKTIIE
                     NPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGELKIFKYINDT
                     EIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVKEKEAPNWIDF
                     DPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPSIYFKLFRAST
                     NNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGNDYVPSGYQKI
                     ENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQHKEVSEKDDW
                     RYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNISIPGSHITPW
                     KNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITNKSKSILPKTS
                     SKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2409..2894)
                     /locus_tag="PW004_001732"
     CDS             complement(2409..2894)
                     /locus_tag="PW004_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HDL2726705.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    2984..3329
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3374..4015
                     /locus_tag="PW004_001733"
     CDS             3374..4015
                     /locus_tag="PW004_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDL2726706.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            4034..4669
                     /locus_tag="PW004_001734"
     CDS             4034..4669
                     /locus_tag="PW004_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL2726707.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4687..5502
                     /locus_tag="PW004_001735"
     CDS             4687..5502
                     /locus_tag="PW004_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2726708.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            5821..7257
                     /locus_tag="PW004_001736"
     CDS             5821..7257
                     /locus_tag="PW004_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL2726709.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7446..9893
                     /locus_tag="PW004_001737"
     CDS             7446..9893
                     /locus_tag="PW004_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDL2726710.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10123..11190
                     /gene="rlmN"
                     /locus_tag="PW004_001738"
     CDS             10123..11190
                     /gene="rlmN"
                     /locus_tag="PW004_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HDL2726711.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(11383..12486)
                     /locus_tag="PW004_001739"
     CDS             complement(11383..12486)
                     /locus_tag="PW004_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HDL2726712.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
BASE COUNT         4389 a         2169 c         2723 g         3583 t
ORIGIN      
        1 aatgttattt attattacta atataacatc actgattcct gttcatgtct atgcggatgc
       61 aggcagagat atcagcagta atgtcacttc gttgaccgtg gacccaacga atattacaga
      121 tggaggaaac ataaaagtaa aattctcgtt tgacgaaaaa aaacaaaata tccaaccagg
      181 tgattatctt tggatcaatt ggccaagtga aggaaatatt cgcggtgaag gattccaaaa
      241 agaaatccca ttaatgattg aaaataaaaa tgttggaact ttaacggtca gaaaagattc
      301 tgcgcaggtt gtgtttaatg aaaatattaa aaacctagac tcagtagaag gttgggggca
      361 atttgagata caggccagaa acgtaaccga taccggtgaa gaaaatacgg gaccattcac
      421 cgtgacgagc ggtgataaaa cagctactgt aaatgtgaca aagccagctt ctggttcttc
      481 ttctagcgtt ttttattata aaacaggaga catgctacct gaagatacta agcacattcg
      541 atggtttttg aatattaaca ataacggcac ttatgttgaa caaccagtta aaatcagtga
      601 tgaaatccaa agtggacaaa gattagatcc aagtacgttt gaaattaatc aaatacactt
      661 aggtgaacag aaggtttata gaggagaaga gggaatccaa caatttttac aagatttccc
      721 cagcgctaca tttaattttt cagtaactga taattacatt gaaataacta taccaaaaaa
      781 ctttgtcaat cttaggaaaa taatggtgag ttataagacg atcatagaaa acccagaaca
      841 aataaatttt gagaatcatt cggaagcttg gtttaaagaa tttaataaac ctgctgttga
      901 tggagaaagt ttcaatcata cagttaaaaa tatttcagct tctggtggag tcaacggtac
      961 agttagaggc gaattgaaga ttttcaaata tatcaatgac acagagattg gtatcccaaa
     1021 tgtgactttc gaattaagac gagcagatga acagcctatt caaggacagt ctagtatcct
     1081 gttgacttcg aatgaacagg gagaagctag tatcaaaggc ctgcaagtag gagactatgt
     1141 tgtaaaagaa aaagaagcac ccaattggat tgattttgat ccactaagtt caaatgaact
     1201 caaattctcc ataaacgaaa atgatacaga gggagtcagt ttacctattt ataacaagaa
     1261 aaaggttacg aacatcacag caactaaaat ttggaacggt ggaaccactc caagaccgag
     1321 tatttatttt aaattattca gagcaagtac aaacaattgg gaaccagttc ctgatgcaga
     1381 aacaaaacga ttagacaatg gaataacttc agtgacttgg gaagatatcc aacagtatga
     1441 tgatagtgga aacgaatata cgtttaaagt gcaagaagta gatcaaaacg gaaatgatta
     1501 tgtcccttca ggttatcaaa aaattgaaaa cgggctagtc gttacaaatg agaataaaga
     1561 agtcatttca gtctcaggac aaaaaacatg ggaagacaat gagaaccaag acggcaaacg
     1621 accgacgacg atcacagtaa atctattagc cgacggtcaa ccgatccaac acaaagaagt
     1681 aagtgaaaaa gatgattggc gctatcgttt cacaaatctg ccaaaataca gagacgggca
     1741 agaaatcatc tatactgtaa cggaagacaa cgtgcccgaa tacagtacga cgattgacgg
     1801 gtataacatc aaaaacagtt atacaccgga aaaaacaaat atatctatac caggtagcca
     1861 tataacaccg tggaaaaatt ttccagagaa gacagagaga gagaaaatta gaaatctatt
     1921 ctctcaaaaa ttgcaaatat ttgctcatac aggagaatcc agcgaacaaa gaagcaatta
     1981 tattagtaag agagctgtac ctaaacagat aacaaataaa tcaaaaagca ttcttcctaa
     2041 aacaagtagt aaaaaatcta gttttgctgt aatcattggt ttattgatag taactatctg
     2101 tgctgggata ttcttacaaa aatataaaaa agtttagtta gaatataata ttttttgtta
     2161 aatatgactg aaatcaaaag aataaaattc cgatttataa tcgaaaaaag aagcattttt
     2221 atatatgctt cttttttcgt agaatcaata aatatggatt tggctaacta tggaagaata
     2281 ctcgtattag ttttcagcgt tcatactagt tgttacattg tttgattggc caaaagaatt
     2341 tctgatatat tttgtgctaa ataaagaaaa agacgcttga attgggaaat aagaaaacta
     2401 ctctatcatt acaaattttc ataaactggc agatgttcta agtaaacaag tatcttttca
     2461 aagtcggaaa aatccataat caacgtagtg gtattgatat ttggatgaaa gcctacggta
     2521 tcttgacgat caatagcagc atcgatcacg acttggattt ttttctcttt gtcgtgcatc
     2581 aatgctaatg gagtcactgt acctgctgga acgcctagga ggtcaggaag ctgttcttca
     2641 gaaacaaaag ataagcgctt ttcttttaat tgttcagcta gttgttttaa atcggcgtgc
     2701 ttttcatcag gcaagatgac aagatagatt tgttttcctt ttcttgattt caataataga
     2761 ttttttactt gcggaccagg tagggaaaaa gggacatttt tgactgacgt aatcgcagga
     2821 tgatcgactt tttgaaaaga gatacccagt ttttttagta gttcataagc ttcttgttcg
     2881 gttgcacgac tcatcgcgca tcaccctcct ttttttatta gtgtatcata aaaaagagtt
     2941 gacaaatgaa aaataatcat gtaaattaca cgtaaagtta aaacaaatga catcgaaaag
     3001 aagagtagcc gttaggtgtt tttagagagt ctctggttgg tggaaagagg cagcagttaa
     3061 ccgtgaaaca catcttggag tcgattttct gaagcgtata gtaggaaaat ccggggagcc
     3121 cgttacagct gcggtcgttg ctagatgacc agataaggct ggcttttccg tgaggagtcg
     3181 gtaaaaaaag gtggaaccac gtgattaacg tcctttgatc ttcggatcaa aggacttttt
     3241 gttttcatct gaacggcaac gactgtgtga ggttttttct gcaaggaaaa aataaacaga
     3301 ggtggaacca cgtataatac gtcctcttgg catttgccaa gaggattttt ttattttaaa
     3361 ggaggaaaag aagatggagt atttgattga agttatgccg caattattag taggagcact
     3421 cacaacattg aaattatttt tatttacctt gattggctca ttaccattag ggattgtttt
     3481 agcattagga ttgtctttgc attttaagcc gctacgctac ttgcttcaaa catatgtatg
     3541 ggttatgcgg ggaacaccgc tgttgttgca actgattttt attttttatg ggttgccgac
     3601 gattgggatc gtttttgatc gtttcgaagc agcactcatt gcctttatct taaactatgc
     3661 agcttatttt gcagagatct ttcgtggagg gatacaatcg attcctcaag gtcaatatga
     3721 agcagcgcaa gttttgcaat taagtccctt tcaaacggtg acgaaaatcg tcatcccaca
     3781 agtgacaaag attgttttac cttctgtagg gaatgaagtt atcaacttgg tcaaagattc
     3841 ttccttgatc tatatattag gtttaggtga cgtaatgcgt gctgggaaaa tcgcgatgga
     3901 acgcgacgcg actttactgc ctttgctagg cgtggcaatc atctatctta cattgactgg
     3961 tattctaaca atcgtaatga aacaagttga aggaaaatta aattattacc gctaactgat
     4021 aggaggaaga aatatgattg aattgaagaa agtgaacaaa gcatttggtg aaaaaaaaat
     4081 aatcaaagat ttgaatctaa tcattccaga tgggaaaaca ttagctatcg taggtccttc
     4141 cggtggcggt aaaaccacgc ttttacgtat cttggctggc ttagaaagtt ctgacagcgg
     4201 agaattttat ttagacggtg taccgttcaa cccattcgag cttaaaatga aagaacaagt
     4261 tgtaggagtc gtattccaag acttccagct atttccacat ctgaatattt ttgaaaacat
     4321 taccttagca ccaaaaatgg tattgaaaca gacaaaagaa gaatacacca aaaaagcaga
     4381 agaattagcg gcttcattag ggattagtga tttattgaat atctatcctt atcagctttc
     4441 tggcggacaa aaacagcggg tagcgattgc cagagcattg gctatgaatc cacgggtatt
     4501 ggcttatgat gaaccaacaa gtgctttaga tcctgaatta cgccagcaag tcgagcgttt
     4561 gatcctatca ttgaaagaag atggggtaac ccaaattatt gtcacccatg atttagtatt
     4621 tgcagaaaat gtcggtgatc aattactaaa agtttcccca attcattaag aaaggagaag
     4681 atagagatga aaaaaaccag ttttctattc gctttgatcg caggtcttct tctttttgca
     4741 ggctgttcga acaaaaaaac ttcagcagat aattgggcga ctatcaacga agagaaaaaa
     4801 atcgttatag gtttagacga tacatttgtt cctatgggat ttagagataa agacgggaag
     4861 ctcacaggat ttgatattga tttagccaaa gcagtctttg aacaatatgg gattacggta
     4921 gactttcagc ccattgactg gtcaatgaag gaatttgaac tgaacaatgg aacgatcgat
     4981 ttgatatgga acggctattc caaaacgccg gcaagagaaa agaaagtgca atttacaaaa
     5041 ccttatatgg aaaacgatca agtgctgatc acacctaaag aatcaggaat cacaaacttt
     5101 ggacaaatga ataataagag attaggtgcg caaaatgggt catctggtta tgatgtattt
     5161 acgaaacaac cagaagtatt aaaagagata gtcaaaaacc aagatgctgt tttgtatgat
     5221 agttttaatg aagcattgat cgatctgaaa agcagacggg tcgatggtct gctgatggat
     5281 aaagtatacg cagactatta tttgaaacaa agaaacgaac tagaagactt caacataaca
     5341 aaaggttctt atgagagtga ggattttgca gttggtgcac gcaagtcaga tagtacatta
     5401 gtgaaaaaaa tcaatgaagc cctagatcaa ctagagaaaa caggtgaatt ccaaaaaata
     5461 tccgataaat ggtttggcga agatgttacg ccgagaaatt aacaaatagg ggactgtgac
     5521 aaaagacgtg tcacagtccc tttttccgaa taaacagtgt tctcctgcca actccttgtg
     5581 actcggagct gaatggcagg ctcacaacct cttggtaaac ggtgttcaaa agccagtttt
     5641 tcggtattaa gccaaattta tgcaaaaatg tagaaagcca ttttcacaaa atttttctta
     5701 ctactcgaaa ctaagcgact ttttcacaac ctcctttaca taattttatt ttgttgtgga
     5761 aggggtttcg tgttattcta aaagaggaac gattgaaggt tataggaaag gaagagatga
     5821 atggaacaca aagaacttac ctcatttcca aaagattttc tgtggggctc tgcttctgca
     5881 gcgtatcaag tagaaggagc ttggcaagaa gatgggaaag gagaatcagt ctgggatcga
     5941 tttgtacgga tacctggaaa gacatttaaa ggaacgaacg gtgatttggc agtagaccac
     6001 tatcatcgtt ataaagaaga tattgcttta atgaaagaac aaggtctgaa agcttaccgc
     6061 ttctctgtag cttggacacg gatttttcct aatggacgag gagaagtcaa tcaagctggg
     6121 ctcgcttttt atgagagatt gatcgacgaa ttgattgaaa acgatattga acctatgttg
     6181 actttatatc attgggatct tccacaagca ttgcaagatg aatataatgg ttgggaatca
     6241 cgtcagatca ttgaagattt cactcattat gcagaaacat tatttgaggc attccgcggg
     6301 aaagtccatt actggattag tttgaatgaa caaaatatct tcacttcatt aggttatttg
     6361 ttagcagcac atccgccagg tgtgacagac ccgaaacgaa tgtatgaagt taaccatatt
     6421 gctaatttag caaatgcatc tgtcatcaat aaatttcacg agttagaaat gcctggaaaa
     6481 atcggaccaa gctttgcgta tacgccaaat tatccaattg acagtaatcc tgaaaatgtt
     6541 ctggctgctg aaaatgcaga agacctgatg gccaattatt ggatggatgt ctatatgtgg
     6601 ggaaaatatc cgattgctgc catgaggttc ttagaagaaa aaggatgggc accaacgatt
     6661 gaagctggcg atgcagaatt gctagaatct gctaaaccag attttcttgg gatcaattac
     6721 tatcaaacgg cgacgaatgc attcaatcca ttaaacggag ttggtgctgg aaaaatgaat
     6781 acgacaggaa aaaaaggttc atcagaagaa actggagttc ctggaatgta taaaaaagtt
     6841 gagaatccat ttgtcgaacg aacaaattgg gactgggaga ttgacccaga aggattaagg
     6901 atcggactgc gtcgtatcac aagccgttac cgcatgccgg tactgattac ggaaaatggc
     6961 ttaggagagt atgacaagtt gacagaagat aaacaaatcc atgacgacta tcgaatcaat
     7021 tacttacaaa gccacgtcaa agccatcaaa gaagcaattt cggatggagc agaagtccta
     7081 ggctattgca cctggtctta tacagatttg cttagctggc tgaacggtta tcaaaagcga
     7141 tatggatttg tatatgttga tcaagacgaa acacaagaag gatctttgga acgatataag
     7201 aaagacagtt attactggta ccaaaaactg attgaagaaa atggagagaa cctttagttc
     7261 acatacttaa aacaagaagg tacggctgga tatggccgtg ccttttcttg ataaaatcat
     7321 ttagaaatta agaaaaactg taaaataagc tgaaaaatga aagaaagcgt cagttctttc
     7381 aaacaaaact ttagttccct tacttttttt tgtataatga aacttgatga aatgagggat
     7441 aataaatggc acaaaagaaa cggcctacta aaaaaaggaa aaagacgaaa aaaacgaaaa
     7501 agcaaaaaca gcagcaggaa caaatgctga cgattggtgt tgggtttgcc ttgattttat
     7561 ttgctgtctt cggcttttta aaattaggtt ttttaggaat attgattgca aacggctttc
     7621 ggatcattgc aggaaatact taccaaatcc tctgtctttt attagccgta ttaggatttt
     7681 ggattgtcat taagaataca gagttttcaa ttggtaaaaa ccgacgctgg tttggcggca
     7741 tactctttta tttcggtatc ttattattgt tacatgccca tttatttggc aaactccata
     7801 ctggggaacc aaatatcatg gggacgacat gggatttgtt agccagtgat ataaaacaaa
     7861 gtcaagtaga caataacgtt ggtggtggga tgattggagc aatcctttat cattttactt
     7921 acttcttgat tgcacagcct ggaagttatt tagcagcagt gctattactg gcaggaggag
     7981 cattcctgtt cagtaatctg gaaggctatc agctgttaaa tggtatccaa tcaattggag
     8041 aacgagtaca ggaattattg gaaggtgatc ctgccaagca agctcgaaag caagcggcaa
     8101 aggaagagag aatgaaacag cgcgcagaag cgaaagaagc aaggcgctta gctgcaaaag
     8161 aagctgcaga aaaagaagct gcagaatatg agaagaaaaa agcggtgagc cagcaaaggg
     8221 aaaatactgc tgatgaatgg caggaaccag aaaatacaga gccggagcag ctaagttttg
     8281 ttccaatcga cagttttcaa gaaaatatcc atcctgcgaa tctagagaaa ccagtaccag
     8341 atacaccaaa acagaccaat acagcagaag gtttttcaga tgagttgcca gaggatgacg
     8401 gtacctcatt agaatttgag attgaagcag aacaggaaaa tcaagactat gaattaccga
     8461 cagttgattt gcttgattct ataccaactg tggatcaaag tgatgagtat aaaaaaattg
     8521 aaaaaaatat cggcgtatta gaacaaactt tccaaagttt cggtgtcgat gccaaggtcg
     8581 tcaaagccag cttaggacca tccgtcacaa aattcgaagt ccagccggct gtaggggtaa
     8641 aagtaagtaa aattgtgaac ttaacagatg atatcgcgct tgctttagcg gcgaaagatg
     8701 tccggatgga agcaccgatt ccaggtaaat cactgattgg tattgaagta ccaaatggaa
     8761 agatcagtat ggtttccttt agagaaatca tcgaagcaca accaaaccac ccagataaat
     8821 tactagaagt acctttagga cgtgatgttt ctggacgggt acaaacggct gatctttcga
     8881 agatgcctca cttactcgtg gctggttcga ctggtagtgg gaagtcagta gcaattaatg
     8941 ggattatcac aagtatatta atgcgtgcta agccccatga agtaaaactg atgatgatcg
     9001 atccgaaaat ggttgaattg aatatgtaca acggcatacc tcatctgttg acgccggtcg
     9061 tgacgaaccc tagaaaagca gcgcaagcct tacaaaaagt agtacaagaa atggaagaac
     9121 gatatgaaaa attcgcggca accggagtaa gaaatatttc tggttacaat gaatttgttc
     9181 aacagaaaaa cttggaaaac ggaacgaaac atccaacctt accattcatc gtagtgatcg
     9241 tagatgaatt agctgatttg atgatggtag ccagcaatga agttgaagat gcgattattc
     9301 gtttagcgca aatggctcgg gcagcgggga ttcatatgat cctagctact caacgtccaa
     9361 gtgtggatgt tatcacagga atcatcaaag caaatgtacc ttccagaatg gcatttgcgg
     9421 tatcaagcgg tacagattct cggacgatca ttgattctaa tggggcagaa aagctcttag
     9481 ggcgaggaga tatgctattc ttgccaatgg gtgagaataa accgatccgt gtgcaaggag
     9541 cattcatttc tgaccacgaa gtagaacggg tcgttcaatt tgttacagat cagcaagaag
     9601 ctcattatga agagaaaatg atgccgacag atgaagttga aacgacaggt gctccagaac
     9661 agccacaaga tgagctgttt gaagaagcaa aagcacttgt cgttgaaatg caaacggcaa
     9721 gtatttccct tctccaaaga agatttcgga tcggttacaa ccgagcagca cgtttagtag
     9781 atgaactgga agctcatgga gttgttggcc cgtcagaagg aagcaagcct cggaaagtat
     9841 tcattgagca ggaagaaaca tcagaaacgt tgccggacga taatgacatg taaaacttgt
     9901 gtgatgaagg gttgaaaaac gaataaccaa taaaaagaca aggcatgatg ataccaaaag
     9961 aaaaaatggt ttttttccat cttttctaat acggtatgat gtcttgtctt tttagttttt
    10021 gtgagattaa agttctattc gatttatttg tgaagtattg cattaaggaa atgaatcatg
    10081 tatagtatgt agcgaaactt tgagaaaaag agggaaaata ctttggaaaa acagtccatt
    10141 tatggcttga cgaatgaaga attaatcaat tggtttatag aaaacggcga gaaaaaattc
    10201 cgagctgccc aagtttggga atggttgtat caaaaacgtg taagtaattt cacagaaatg
    10261 acgaatctat cgaaacaatt gatcgaaaaa ttatcggagc attttatcat caatcctcta
    10321 aaacagatgg tcgtccaaga agcttcggat ggtacagtaa aatatttgtt tgaactccct
    10381 gataaaaaca tgattgagac tgtactgatg agacaagaat atggcttgtc agtttgtgtg
    10441 acgacacaag taggatgtaa tatcggctgt acgttttgtg caagcgggct gctcaaaaag
    10501 aaccgtgatc tgactgctgg agaaattgtt gcacagatca tgatggtcca acattatttt
    10561 gatgagcgca atcttggcga acgcgtttca catgtcgttg taatggggat cggtgagcca
    10621 tttgataatt acgacaatgt aatggacttt ttgcatatca tcaatgatgc caaaggattg
    10681 gcaattggtg ctcggcacat tacagtttcc actagtggat tagcacacaa aatcaaggaa
    10741 ttcgctaata acggcttgca agtcaatctg gctatttcct tgcacgcgcc gaataatgag
    10801 gtgcgaacat cgatcatgcg gatcaaccga agtttcccaa ttgagaaatt gatggaagct
    10861 gtggatgagt atttagagaa aacaaaccgt cgaatcacgt ttgaatatat catgttgaat
    10921 caagtaaatg atagaccaga gcatgcgcaa caattagcag atctattaaa agataagaaa
    10981 aaattagcat atgtcaactt gattccatat aatccagtca gtgaacatga tcaatattct
    11041 cgcagtccaa aagctgatgt attgagattt tatgatgtat tgaagaaaaa cggcgtaaac
    11101 tgtgtgatac gtaaagaaca tggtacagat attgatgcag cttgcggaca attgcgaagc
    11161 aagcaaatga aaaaaacagt tgccaaataa tcgtatccta ctcgtttttt caattaccat
    11221 aaaaggagca gttttcttaa ttaaggaact gttcaactta aataagagaa aagattctat
    11281 agcgaaagct ataaagcttt ttttgacaag aaaaaccctc atcaagcaaa gaaatgtcct
    11341 caatttgatg acagcctgct tgatgggggt tttagcaatt atttaactga ttttcttata
    11401 tccttgctgg ctttcttgtg tagcatgata agtttctacg tcaagaatgg cctgtttaaa
    11461 tttttctcgg gtgatttcat aaggcacatg tttaatatca tttgttgcca tggctttatc
    11521 taagattgct tctaaatcgg tcgtgacatt gagattcatg tcggttaacg atactggaag
    11581 ttggttcttt ttcataaaca catagatttt atcaaacaaa tcgaactgct gatccatcat
    11641 caataaggtc agtacaccat aactgacaga tgctccgtga aggtatccct ccattttttc
    11701 tagaacagtc gtaccgtaat agaatgagtg tgccatagca gaattatagt tattgttaac
    11761 taaaacagaa accaatccgg tagtaacaat gatatccgaa acgacttgtt caattgcata
    11821 tgagggacga tcgtcggcac tatctttcaa tgcttgcgta ccataagtca agagacgatc
    11881 aatgcacatt tttccgattt ggattcctaa tctatccgtg tagcctagat catcgccacg
    11941 agcagaaaaa gtagattcga cttctttgga taaagcatcg ccaatacctg cccataagta
    12001 tttatcaggt gcttcggcaa taattgctgt atcaatgaag gtgtgtacag gtggttttga
    12061 aggaaagaag agtccgtcca ttacgccttc ttcgttatag atgacacata ccgcagtgac
    12121 gggcgaacag ttcgaaccaa tagtgggaaa ggtgaagact ggtttagcta gtttttcacc
    12181 aatgactttt acagtatcgc aagcacggcc accaccgact gcaaacagga cgtctgcttt
    12241 ttggacactt tcatgctgga tcaactgttc aatatttcca taagttgaat tacccccgta
    12301 ccagagactg gcgacaacct ctatttcctg tgtcaaagct acttttattt tgttttctgc
    12361 ctttgaaagt gcagtcttcc ctccaatcag cactactttt gtcccatatt ctttgacgat
    12421 tccatttgct ttttcatacg catctgttcc aatggtataa tttggtaaga aaacactttg
    12481 actcatattc atttccttct ttcttcgttg aaacgggtta aaattttaaa gagtgatcga
    12541 tctgactgcg gctgtagatg gagtagaggt gaacctaaga caggataagt gatgaataag
    12601 gtggcatgaa caccttgaaa tcatcaataa gtatcctaaa aacgaacaag tacaaaaaca
    12661 ttgaaaacga caataatgaa tttttgttcc tcttagtatt tctaccgatt ccctttgctt
    12721 ttttgtaatt atctaaaaat tctaaaaatt aagattatta tatgaaaaca ggttttattt
    12781 gtcaattcat aattggtttt ttttaaagca aaagataatc tatatcatga tgaacaaaca
    12841 gagttttttc ctccaattga tggc
//
LOCUS       DANKKG010000073        12724 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000073 DANKKG010000000
VERSION     DANKKG010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12724
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.073"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(76..255)
                     /gene="rpmF"
                     /locus_tag="PW004_001740"
     CDS             complement(76..255)
                     /gene="rpmF"
                     /locus_tag="PW004_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDL2726713.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(313..882)
                     /locus_tag="PW004_001741"
     CDS             complement(313..882)
                     /locus_tag="PW004_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HDL2726714.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1068..1970
                     /locus_tag="PW004_001742"
     CDS             1068..1970
                     /locus_tag="PW004_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL2726715.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2005..3789)
                     /gene="pyk"
                     /locus_tag="PW004_001743"
     CDS             complement(2005..3789)
                     /gene="pyk"
                     /locus_tag="PW004_001743"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDL2726716.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3863..4825)
                     /gene="pfkA"
                     /locus_tag="PW004_001744"
     CDS             complement(3863..4825)
                     /gene="pfkA"
                     /locus_tag="PW004_001744"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDL2726717.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(4993..8307)
                     /gene="dnaE"
                     /locus_tag="PW004_001745"
     CDS             complement(4993..8307)
                     /gene="dnaE"
                     /locus_tag="PW004_001745"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDL2726718.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8538..8723
                     /locus_tag="PW004_001746"
     CDS             8538..8723
                     /locus_tag="PW004_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HDL2726719.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8785..9981)
                     /locus_tag="PW004_001747"
     CDS             complement(8785..9981)
                     /locus_tag="PW004_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDL2726720.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10151..11587)
                     /locus_tag="PW004_001748"
     CDS             complement(10151..11587)
                     /locus_tag="PW004_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDL2726721.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11880..12689
                     /locus_tag="PW004_001749"
     CDS             11880..12689
                     /locus_tag="PW004_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL2726722.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3799 a         2673 c         2250 g         4002 t
ORIGIN      
        1 ttaccgacta caaaaaaata aacctcaagg ctccatggtt agccttgagg ttttacattt
       61 tctaattaaa atgtattaag cttcttttga cattacgtcc ttaccatcat agtaaccaca
      121 atttgcacat acgtgatggc tttttttcat ttcaccacag tttggacatt cgtttaaccc
      181 tttgattgtc aatttgtagt gtgtacgacg ttttgctttt ttagcttttg atgttcttct
      241 agctggtact gccattttgc tacacctcct tgtaaagtct tgtatctaaa ttagacacaa
      301 tatattccaa tcttacttgt tatcgtcgtc ttttgattta gaatcaaaaa gttcagatag
      361 ttttgcaaga cgaggatcaa ttttattctc ggcttcttcc atttttctgc gctggtattc
      421 ttcttcggaa acaacttccc aatcattgcc ttttggaagt tctgttgctg cttcctcttc
      481 ttttgtcaat acccgcattg gaatcgccaa taagatgttg tcttcaatcg actctgctaa
      541 attcaataat ggaccttcaa ttagcaaaat ctcatctgca gacatttgtt catctctact
      601 ttggaattgt tcttctgtca tgaatacttc gtctacagaa aaatccatgg gcagttcaac
      661 aggttctagt gaacgtgttg aaggaactgt caaagtcgtc tgcacagtgt aatgaacgat
      721 atagcctttc ttatcaactg agaccaaacc ttcgacttcc actggcgcaa catcaagtat
      781 taaattgtct cgcttcatga gagcggcttt caagtcgagc gtctcatgaa aagttaacgg
      841 tgtctcctgg tattttctca attccaataa agaccatttc atttttcatc actcctaagc
      901 aacaaaagca attatacagg gcaaaaagca tgttgtcaat gaaattttct tgacaccttg
      961 cagttcataa aaaaacagaa aaatctttat tagaaagcct ctgtttgcta tcatcctcag
     1021 aatcatttac aataatcatt attccattaa aggagcggat gatcttcatg aacacgaaag
     1081 atatcgaata ctatgtccaa atcgtcaaac aaaaaaattt tacgaaagtg gctaattatt
     1141 tccgtgtgag ccaacctact attacatatg cattacaacg tcttgaaaaa gagtttgaca
     1201 ccgtacttat tacaagagat cgatcgcatc acgaattaat cgtcacgcct tctggaaagc
     1261 agctgtttct ccatgcacaa accattctcc aagagatttc tactgcaaga gctgaaatca
     1321 ctcagttgca aaagaaaaag cttcgttttg gcatgcctcc tattatcggc aatgaatact
     1381 ttcctaaact gtcaagttac tttatcaagc atgaattgat gaatcaaatt gagattaccg
     1441 atggaggctc tcgggactta tacgggatga tccgacaagg gaggatcgat ttagctgtat
     1501 taggctcaac acagccaatc gtggatcaat atttagaaac agagttactt ttggacaaac
     1561 gtttcatgat cgtcgtgcca cctacacatc ctctttccaa gcaggatact gtctctttca
     1621 aagatatagc agatgaacag tttgtactat tgaacgaaca ttatgtccac cccgcagcat
     1681 tcactaagct ttctaaacaa acacatactc atccaaaagt tgtttatcaa agcaatgact
     1741 tgacgatttt aaagagtatg gtccgagaaa atgtgggtat cggttttttg gcagagattg
     1801 ctgtccatcc tgaagatcat ttagtttgcc tccctctttc agatgaagtt cagcctcgct
     1861 tcttgatttc attggcaaaa cgaacacagc agatcgtcac gcctcttcaa gaacaagtag
     1921 tccaagttat ccatgaattc gttacagcac aaaaaaacag ctcttcgtaa aagaagagct
     1981 gtttttattt tttattaagc taaattagat agctgttgtt tcgccacggt aaacgatgcc
     2041 gcgacgtgaa tcaacggtaa tcaattcacc ttctgtaatt gttgttgttg cgcctttagc
     2101 accaacgatt acaggaatat cttgtgcaat tcctacaact gcagcgtgag aagtcaatcc
     2161 gccttcttca acgataacag cagctgcttt ttcgattgca ggcatatatt ctttgtcagt
     2221 tgatggtaca actagaacca ttccgtcttt tgcttttttt acagcttctt cagcagatga
     2281 agcaataact gcatttgcaa cgacagattt ttgtccaacg ccttgagctt ctaataattt
     2341 agaaccaatc atttgaattt tcatcacgtt agttgttccg cgttcaccaa ctggtacacc
     2401 agcagtgatc aagattaaat cgccttctga tgcgaaacct aattcaacag cttttttagc
     2461 tgctagttca aacatttcat cagttgttga tggtttttca gcaactgttg ggtaaacgcc
     2521 ccagtttaac atcaaaccac gttttgtgcg ttcgtcaaaa gtaacagcca agatatctgc
     2581 atctggacga tattttgaaa tcatttttgc agtgtatcct gattcagttg cagcaacgat
     2641 tgttttcacg cctagatttt ttgcagcacg agctacagat aaaccgattg tttcagttac
     2701 atctgtttta tcgaattcgt tcaattggaa tgtacctaat tctgataatg cattttcagc
     2761 ttcttggtcg atacgtgcca ttgttgaaac agcttcaact gggtagtccc cgtttgcaga
     2821 ttcaccagat agcattgttg catctgtgcc atcaaataca gcgttagcaa cgtcagaagc
     2881 ttccgcacgt gttggacgtg ggttttcttg cattgattct aacatttgtg tagcagtgat
     2941 aactgggata ccagcagcat tacatttttt gataatgcgt ttttgaacca ttggtactag
     3001 ttcagctggg atttcaacac ccatatctcc gcgggcaatc ataacaccgt cagaaacttt
     3061 gatgatttcg tcaatgttgt cgataccttc ttgagattcg attttaggga agatttgaac
     3121 atgagtcatg tctttttctt ctaagatttc gcggatatct agaacgtctt gtgctttacg
     3181 tacaaaactt gcagcgataa agtcgatatc gttgtctaaa ccaaaacgaa tatcgtcagc
     3241 atcttttgga gtaatacctg gcaaactaat agaaacgcct ggtgcattta cccctttacg
     3301 tgatcctagc atacctgcat ttttaacttc tacgactaat tcacggtttg cttcgtcttt
     3361 ttcaatgatt tgcatatcga tcaaaccatc atcaaataaa atgtgtccgc caacgtgaac
     3421 atcgtcaaat aatcctgggt aagtaaccgc gattttttct tttgaaccta catgttcagg
     3481 atccatagag atgcgtgttt gatctcctac ttcaaatttg atgtatcctg cacgaccaaa
     3541 atcagcttct gttgtgcctt gaacagttgt acggatttca gctccttttg tatctaaaag
     3601 aataccgaca tcttttcctg tctttttaac tgcttcacgg accatagtca tacgtgataa
     3661 ttgttcttca tggtcaccat gagaaaaatt aaagcggaag acatttgccc cagattcaat
     3721 caattgagaa ataatttcta ctgagttact tgctggtcct aatgtactaa cgattttggt
     3781 ttttttcatt taaaaaactc tcccattcaa ttttgtttat ttaaagctgt acacagctta
     3841 aatagcttgg tttctttcac tcttaaaatg aaagtgaatt atttagatca tatagtgata
     3901 agtctggttt atgtttgttg ttttcaagtg tatcaatgat gtcagacgca acgatttcgt
     3961 tgtcatggat accgacacat aatccacctt ttccttcttg aagcaattcg acagcgtaag
     4021 caccgaattt acttgccaac acgcggtcgc gtgcgcttgg tgctccacca cggacaacgt
     4081 gtcccaagat agatacacgt gtatgatagt caccaaattc tgaaagtttc tcagcgaatt
     4141 catttccgcc cattacacct tcagccaaga tgatcaagca atgtttcttc ccgcgatcgc
     4201 ggccttcttg gattttttta gcaactgaag ccatatcaaa atcatgttct gggatgatga
     4261 tttcatccgc tccgccagcc acacctgacc ataaagcgat gtctcctgca ttgcgtccca
     4321 ttacttcgat aacgaatgta cgaacatgag aagtcgctgt gtcacgaata cggtcaattg
     4381 attctagtac tgtattgatt gctgtatcaa aaccgattgt gaaatcagtc ccaggaatat
     4441 cattatcgat tgttccagga atacctactg ctgggaaacc gcgttttgtc aaagccattg
     4501 cgccatgata agagccgtct cctccgataa ccactagacc ttcgattccg aattttttca
     4561 attgttcgat tccttttaat tgaccttctt cagttgcaaa ttctggataa cgtgcagaat
     4621 acaagaatgt accaccacgt tggatcttgt cacctacatc tgccacatca agacgacgaa
     4681 tatctccagc aactaaaccg gcaaaaccat aattgatccc gtatacttcc agtccttcgt
     4741 aaataccttt acgaacaacg gcacgaacag ctgcattcat tccaggtgcg tctccgccac
     4801 tggtcaaaat tccgatgcgt ttcataataa acattcacct cattaaaata ctttatccta
     4861 agtatgactt aggtttacaa ccatttttgc gttaaccacg actaacattc taacattatt
     4921 tttctcactt gtcttgattt acggataaaa aaatgaaaaa aaatgaaagc aagtagtatt
     4981 ttttaccgta agttatttga aaaccacatt atcttcgcca agcagataaa taagttgccc
     5041 ctttatactc ttgctgtcat ccacccaata agcatctgac agaactactt ttcgttgcgt
     5101 tttttcgtga tacataacta ctggtatata tcctgggtgt ttctgaaaaa tcacttgtaa
     5161 ttgtttcatt atctcgggat cttcccgttc ttctgtaata cgcaaatagc aagtttgtga
     5221 aggatactgg gcttctaact ggttaggatc agcaaagctt tctccaatta gttgcagttc
     5281 ctgattatat ttgctgattt cgctccgccc ttgaacaaag atcactcgat caacggcaaa
     5341 caaattacga tatttacgat aaagagttgg aaaaactgtg acagaaattt ctccactact
     5401 gtcggttcct tcaacaaagg ccatcagttc tccttttttt gtccgaattt ctctaacttc
     5461 cttgatcatc actaataaat tgactggttg attagggacg atatctgtaa tcagctgaac
     5521 ttgtttagct aaacgaagac gatcaaaccc ttctgcagga tgacctgaca aataaatacc
     5581 taagtactcc tcttccagct tcaagcgttc ttctagtgta tagtcagcga cttcttcttc
     5641 ttttaatgcc atgattccca gcaagtcaag acttccacca ctgtaaacga tattttggat
     5701 ttttccttcg agatcaagca tcaattgttt cctgttagga gataattcat caaaaactcc
     5761 tactgctgtc agtgattgca gtaactctaa tttcagccaa cgcttatcga tccgtatgag
     5821 gaattgatcc actgactgga acgggccatt ttgttttctt tcgtttaata tatcttctac
     5881 aaaatcacgc cgaatacctt ttatcgctgt cagtccgaat ctgatttctt ccttattgac
     5941 caactcaaaa ctggaattac tttgattaat ggacggcgag gaaagtttga gtttattttt
     6001 acgtgcttct gcaatatatt ctttcaattt tgatgtgtta tgccgaacag aattcatcag
     6061 gctggtaaag aaagcacctg gatgatgggc ttttagatac gccatttgga atcccacgaa
     6121 tgaataagca aatgcatgtg accgattgaa tccatagttt gcaaattttt cgatatattc
     6181 atatacggtc gctgctgttt tttccgaata tccctgttgt aatgctcctg atacaaaatg
     6241 cgacttttct tcatctaata cgtctttttt ctttttactc actgcccgtc gcagaatatc
     6301 tgactgccct aatgtaaatc cagccatagt cgaggcgatc tgcatgattt gttcctgata
     6361 gacgatcact ccatatgtgt ttttcaaaat cgggatcagt gaatcatctg ggtagcgaat
     6421 ggcttctttt cctttcttcc tggcaatgaa ggtatctata ttttgcatag ggcctggtcg
     6481 atacaatgca ttgactgcgg caatatcttc gatgttttct ggatctaatc ttcgtaaaac
     6541 gttccggata ccagcagatt caaattgaaa tactcctgag gtttcgcctc gttgaaacaa
     6601 gagcagcgtt tttgcatcat ttagcgggat ctcttttaat cggatctcat acccttctac
     6661 tcgcttaaca gctttcaaag taaaatcaat aatcgataag ttgcgtagtc ctaaaaagtc
     6721 cattttcaaa agaccgattg cttctacctc attcatcgta tactgggtca gaaaagcttc
     6781 atcagaaccg cgttggagcg gaactagttc aagtagattc ttatcgctga taactacacc
     6841 tgctgcgtga gtcgaaacat gtcttggcaa tccttctaaa atcactgcag ttttgaataa
     6901 aagttggttt ttttcgcttg cctgaactaa ttctactaat tgatgcgaag ttttataagc
     6961 ttgggtcaat gtgattttca attgattggg tattgctttt gaccagcggt ttgcttcact
     7021 ttgcgataaa ccaaacactc tagctgcatc tctaagaacc atttttgctg ccattgtgcc
     7081 aaatgttgcg atttgtgcca tatgatattg tccatacttt tctcgaacat aattcaatac
     7141 ttcttcacgt cgattgtcag gaatatccaa atcgatatct ggcatcgtaa agcgttcggg
     7201 gtttaaaaag cgttcgaaca gcaaatcata ttctaatgga tccacatctg tgatttctaa
     7261 cacataggct actaatgaac ccgcagcaga accacgacct gcaccggtaa cgattttgtt
     7321 tctatgagca aaagccatta catcccatac gatcaagaaa taatcatcga atcccatttg
     7381 atggatgaca gataactcgt agtccaaacg ttcttggtat tcctttgtat agtcagtgat
     7441 tcggcgttcc acttgcgaat agcataactc tcttaaatat tctccagctt gttttccatt
     7501 aggaactgga taatgcggaa gtaatttctg atggaactgg aattcaaatg aaatcgattc
     7561 agctaaacgc tgggcgccta ttacagcttg ggaaagaccc gctttttcga atcgtttgat
     7621 catttcttgt tctgaaagaa gatagacatt tgctgttagt ggtaaagatg cagataaatt
     7681 ttctaaaggt atttggcttc cctctttgat atgtttcatc acttcaagtg caaaaagttc
     7741 ttcttgttgt aaactagcga tgggatgtaa tgcacaaagc gaataatttt cttttatcac
     7801 atactgatac cattcaggca attgatcagg cgtgttttct gtcaagccga tataaaaatt
     7861 tctagtaaaa acattttcaa gtaaaaactg ccaatgaccg aaaaagttat cctctttatt
     7921 gaaagcctca gaaacagaat gatttgccgg aacaacaaac gctagatggt cggtttctgt
     7981 catttctgca agttccaatt tttcttggat catttttcgg ctggaaagtt tcatcagctg
     8041 ctgataacct atttcatctt ttgcgtacgc aagcaagcca taattttttt cttcataagt
     8101 ataactaaga tgtaatccaa tgattggctg tactccttct tctacacaaa tacgatagaa
     8161 ttcaggtgca ccgtataaga catctttatc cgttatggct aatgaagtgt agccttgttt
     8221 ttttgcttct tgtacatatt ctcgaatacg tatcgtactt tgtaaaagag aataagaagt
     8281 aattgtataa agttgtggga aaaacatctt gcattccgcc ttttttttcg ttaaaattca
     8341 ctagcgttaa aaggtttgat ccttttactt tcattaagat agcaggcgct gaagttgatt
     8401 attccacttc atttgcttgc ttgtaatctg taggaatgag ttctttcgca agaactcaaa
     8461 ttttattgag atttggtctc gtttctttac attcaaagaa aatctggtaa aattaagata
     8521 gaaaagaggt gttctttatg cgatatatcg ttacattatt ctgggctgtc gttctaggtc
     8581 aagtagtcgg ttatattggt gctgcattaa caagcggaac atatgatttt actttgacaa
     8641 caatcatttc attcattgcg ggtgtcatta tccttttgat tggtgcagtt gcgccaagaa
     8701 aagaaacctc tgcccattcg taaaaaaaca gccaagagct tgagatttct caagctcttt
     8761 tttattttct actcctcacc accattataa tctgctgcga agaactttac gaaagttgaa
     8821 taaagaaaga agaatatttg acagaacaaa caaacttgtc acaataaaaa cagcactgta
     8881 attataagca tgcgcgactg tcgatcctag catgggacct aaaacttgtc caaagttgct
     8941 aaacatctga ttgtagctat agattcggct gactccttct tgaggcgtaa tcttactgat
     9001 cagtgtattg acagaaggca tcaaagcgcc agttgaaaag cctaaaataa agcgaagtat
     9061 acctaattgg agcggtgtag tgacgaatgc cataggtaaa taacagcaaa atgataaaat
     9121 gagtcctcca attagaactt tttgattccc aatcttatcc cctagttttc ctagtttcgg
     9181 tgaagaaaag acagcggaaa caccagctat cgaaacaatc aagccgctga caaaaaggat
     9241 attttctgta cttccgctca attcacgaat atataaggtc aaaatagggc tgatcgtagt
     9301 gattcctatc tgtaaaatca aggtcgtaat gaagagaccg atcaaaatag agagatgatc
     9361 catttttgaa aaaatttctt tggtgcttat cagatctttt ttctcgattg gatggaaatc
     9421 ttctttgact aagaaaatgg tcaataccgt tgtgatcatc aacaaagccc ctgtgatcaa
     9481 gaatacattt tccataccaa accactgagc cagtgcaccg cccattgaag gtccgatcag
     9541 atttccagca atcgctccgg tagacaatgt tcctaacgcc catccgctct tttctctagg
     9601 tgcctgtgaa gcaatcatcg ctgtggcatt tggaatatat ccagacaaaa tcccattcca
     9661 aaaccgcatg atcaaaagcc aatacgcgtt tggtacaaaa gccaaagatc ccattgttaa
     9721 tgtcatccct gctgctgcac ggatcatcat gagttttcgg ccttttcgat cagctagatt
     9781 tccccagatt ggtgcaacca cggcagatgc aaaagcagtc acagagattg ctagtcccga
     9841 aaacaattcg atttgatctt ttggtgttcc tagttgctca acataaactg gtatgaatgg
     9901 cataactaaa ctaatacttg caccagtgaa aaaacagcca atccacgcaa tcactaaatt
     9961 ttttctccaa ttaatctcca tctatcatcc ctctttcgtc tcttttatca tacctatttt
    10021 cgaataaaca agcaacgatc tcgccagtaa aaagagaaaa atcccgcaaa ggtcatttta
    10081 tatgtaacgt tctttttatg taaaacaaaa agagatcaga acaaatactg ttccaatccc
    10141 ttctctttag ttaaattgtt gctagaatga tgaagttgat aatgaataaa atatcaacga
    10201 tccacaaagc aacagaaact tgattgtatt ttcctttgat tactttcaca attgtataga
    10261 aaataaatcc tgctgcaatc ccataggaaa tgctatagca caatcccata aagatcgatg
    10321 caaagaaggc cggtaaagct tcttccatat ctagccattt gatgtctgca aaagaagcca
    10381 tcatcattac tccaactaaa atcaatgctg gcgcagttgc ttgatttggt acaatggcga
    10441 tcagtggaga gaataaacta cttaatgcga atagcactgc cactactact gaagtcaaac
    10501 cagtacgtcc accggccccg atcccagctg cagattcgac ataagttgtt gtattcgatg
    10561 taccaaagat agcccccaca gaagtagcga tcgcatcggc aaatagtgct ttatccattt
    10621 ttgttttgaa cccacgacca tcttccaacg cgatctcgtc ttcttttgag aaaatacctg
    10681 tgcggcgtcc tgtaccgata aatgttccga tcgtatcgaa agtatcagat aaactgaatg
    10741 cgatgatcgt catcaatact tgaggaatct tggaagaatc actgaataaa gattgcatgc
    10801 cttcagaacc aaatgctgca ccgaaagttg ttcctaattc tttaaatgaa ttacctaacg
    10861 aattcgtttg ccaatcaatg gaacttaaat caacaacacc cataaaaata ccaaggactg
    10921 tggtcgctac gataccgata agaatagccc cacggacatt ttttacaact aaaatggctg
    10981 tcagtaccaa accaatcaca gccaataaga taggagcgtt gtcaaagttg gctaacgcag
    11041 ggacgattcc accatttatc gtaacatttg tcgcttttcc gccttcaacg acagagctcg
    11101 aaatagctga ctggtctgct gaaaaagata agaatcctgc atttttgatt ccgacatacg
    11161 caacgaaaat accgatcccg ccaccaatcg catgttgaag actttcggga atcgcccgaa
    11221 taatcatttt tcgaatattt gtgacagtaa taaaaatatt gattaatccg caaataaaca
    11281 ccattgccaa ggcttgttgc caagaataac caagcccaaa aacgaccgta aaagtgaaaa
    11341 atgcgttcaa tcccatacca ggtgcctgag catatggtac atttgcgaac aatcccatta
    11401 ccagcgtacc aataatcgat gcaatgattg tcgctagaaa tactgcttgg aagggcatac
    11461 ctgatgctga caaaatcgtc ggatttacga ataagatata actcatggca aagaatgtcg
    11521 ttactcccgc cattatttca gttgatacat ttgtattgtt tttctttaaa ccgaaaaact
    11581 tttccataaa aagttccact ccctgaattt ttttctgatg gaaaactata ataataaaaa
    11641 ataagtaaaa tagggcaaat ccatcgaaca atttcaaatt atatagacgt ttcattcgct
    11701 tttcaacgtt tttttaacaa aaaaagcgaa aataatactt aacgttcgtg tttagcttat
    11761 gtttagctat ttatatagcc ataactgtag tttttgttag gaatagctga ttgctcttgt
    11821 taattaccta aaatatgcta gactaaaaca aatgctagtt gaggaaggaa ggacccttat
    11881 tgaataaaaa attaattgcc atcgatctgg acggaactac cctcaatcaa gattcgatga
    11941 ttacatctaa aacaaaagag actctaaaaa aagcaatcaa tgccggtcat catgtcagta
    12001 ttgctacagg acgcccattc cgaatgagcc atcagtttta ccaacaatta gaattaacaa
    12061 caccgatggt taattttaac ggcgcattag tacacatccc taatcagcac tgggatggtg
    12121 aaagagaaac gttgatcaat cgtgaaatcg cttttgaaat cttatcgcaa aaaaagcagc
    12181 taaatttaga ttttatcgct gctgaaaatc gtgatacttt ttttattgac agttttgatt
    12241 tctttgatga aaaaattttt gcttcttctc gtccaggaga gaagaatttg ctttctccta
    12301 aaaatttgac gacaaatcct acatctttgc ttgtccgaac agacaaacgt tttgcaggag
    12361 ctgtatctgc tgaattaaca cgtcaatttg gcagttatgt cgatgttcgg acatggggtg
    12421 gacctactgc catcttagag attgtttcaa aaggaatcca aaaagcaaaa ggcgtgcaag
    12481 agattgctaa ctacctgtct atcgatcaaa aagacgtcat tgcctttggt gacgagcata
    12541 atgatttgga attgttggat tatgcaggtt ggggagtagc tatggctaac ggtactgacc
    12601 aactcaaagg cattgcaaat gatgtcactc ctttgagcaa tcaggaagac ggtttagctg
    12661 tttatctgga aaaactactt aaactataat agagaattag attccacaaa aaagaggttg
    12721 tgac
//
LOCUS       DANKKG010000074        12641 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000074 DANKKG010000000
VERSION     DANKKG010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12641
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.074"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(<1..482)
                     /locus_tag="PW004_001750"
     CDS             complement(<1..482)
                     /locus_tag="PW004_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL2726723.1"
                     /translation="MNHKLSETEQYLWNFIEHLILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     "
     gene            624..1556
                     /gene="pfkB"
                     /locus_tag="PW004_001751"
     CDS             624..1556
                     /gene="pfkB"
                     /locus_tag="PW004_001751"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDL2726724.1"
                     /translation="MSIYTCTMNLAIDLFIETDEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAGDYLCVSGSLPRGLSPKVLIEISRICLIIDS
                     SDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENVLLSLGE
                     KGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLAGYMNRRSLDETLRKSIAAGSS
                     TAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            1538..3001
                     /locus_tag="PW004_001752"
     CDS             1538..3001
                     /locus_tag="PW004_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2726725.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFVGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSSDPDHAQYNATAAMVKAVDDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLIILPKQLDGLKAIVLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEYSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLTQGN
                     TTFMAGASAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKRDNKATNRTVIEEGRAGGLS"
     gene            2998..3456
                     /locus_tag="PW004_001753"
     CDS             2998..3456
                     /locus_tag="PW004_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2726726.1"
                     /translation="MTIIHEENIFLQQPLDNQEEAFKFLAKQTFCLGIAHDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLKKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKIEIATILTKEIGEDI"
     gene            3453..4427
                     /gene="lacD"
                     /locus_tag="PW004_001754"
     CDS             3453..4427
                     /gene="lacD"
                     /locus_tag="PW004_001754"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HDL2726727.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDEPVEINVQKQIVIERIGSECEAEGIPFF
                     LELLSYDATIGDPSSLDYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QDTCYSQAEASAYFYEQSKVTEQPFIFLSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATSIYVNK"
     gene            4868..5596
                     /locus_tag="PW004_001755"
     CDS             4868..5596
                     /locus_tag="PW004_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2726728.1"
                     /translation="MESSNKGKSLYYQLVDLLKDRIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELENQGYIYRRHGKGTYVSDLSDKSSNLAGAYSFTEEMREIGRVPKTV
                     ILEFRQMEANKRIAKNLNIHLGETVFKLKRLRLADEEPLMVERSYLPAKLFMGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDAVVLRIADGAPVLHLTRITYNVKN
                     EIIEYTDSVARADQFHYKIRHIRK"
     gene            5614..6810
                     /locus_tag="PW004_001756"
     CDS             5614..6810
                     /locus_tag="PW004_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL2726729.1"
                     /translation="MFELSKNKLKQIGAAITTQEIKQQPDLWKETIDRLMQSQENLAL
                     FFDQIKKEANGRRIRVIFTGAGTSQYIGDTLLPYLNEHGDNQFFIFHSFGTTDIVASP
                     KEFLFPNEPTLLVSFARSGNSPESVAAVQIANEQVETLFHLTITCAKEGMLAKNAQDD
                     KQNYLLLMPERANDAGFAMTGSFTCMLLSALWIFDQTAMEKKERYLEQLVKIGQDLLS
                     RDQEIQKLLPDFFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVVGFVSNQPYTRNYDLDILEEIKEDRVTANVIAIAQPGTIDFSGMTVLLE
                     DSVSKLPDAYLALAMIMTAQIIALYTSIKVANKPDTPSPTGTVNRVVKGVIIHKYETE
                     GEMTDE"
     gene            6803..7798
                     /gene="lacD"
                     /locus_tag="PW004_001757"
     CDS             6803..7798
                     /gene="lacD"
                     /locus_tag="PW004_001757"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HDL2726730.1"
                     /translation="MNEEKNRAMERLSTKDGIISALAIDQRGALKKMIKSLGKEPSED
                     QISEFKELVSAELTPYASAILLDPEYGLPAAKVRAKETGLLLAYEKTGYDATTPGRLP
                     DLLADWSSLRLKEQQADAVKFLLYYDVDEAPEINHLKHVFIERLGSECEGENRPFYLE
                     LVSYDAQISDSSSPEYAKVKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVNGYSTG
                     EFIHSKEEAAAFFKEQSEATPLPFIFLSGGVSTELFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGKDGAIHWLRTNGRENIETLNKVINETATPWFNKTKSKKVKMK"
     gene            7795..8736
                     /locus_tag="PW004_001758"
     CDS             7795..8736
                     /locus_tag="PW004_001758"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HDL2726731.1"
                     /translation="MITTVTLNPSIDIAYTIEELNLDKVNRVETVNKTAGGKGVNVAR
                     VLNLLGEEVIATGLIGGQHGEYLKNQLDQLGIMHQFVPIKGETRDSIAILHSSRQTEL
                     LERGPFITKKEQQTFTDHFEQLLEISDIMTISGSLPQGCPLDYYKQLVALADKKGKDI
                     LLDTSGKFLLEVLEGKEKPLLIKPNLEELGELIGQRICSSIQLEKIQSLLAHPIFEGV
                     PWVVVSLGERGAVAKVAQCFYEVIIPKISVVNPVGSGDATLAGSAHALSKRQSIEEIL
                     AFSMACGMANAQQEKTGWIDPMKVTMLMKHIQVKKIK"
     gene            8912..9391
                     /locus_tag="PW004_001759"
                     /pseudo
     CDS             8912..9391
                     /locus_tag="PW004_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            9404..10294
                     /locus_tag="PW004_001760"
     CDS             9404..10294
                     /locus_tag="PW004_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2726732.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLNAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDRHVEDMNYQAIERNFLMGALPWSLSRMIPVFLAL
                     AFGGGLVENVVSVLNGNLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHLSYLILGFT
                     ITALLATLFSNVQLLGTSVASVVKDLPATFNSLPMLAVALIGLALAMISYKNDQNKDH
                     QPLASEPSHSVEGEIEDDEI"
     gene            10281..11090
                     /locus_tag="PW004_001761"
     CDS             10281..11090
                     /locus_tag="PW004_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL2726733.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDNTPELKEMMKTHTQFFNTSNFFNTIITGIDLAMEEKEGIASKETVSGLKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPIGVFIWIFAQIVVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKILPAGIVGGVYWLLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            11104..11505
                     /locus_tag="PW004_001762"
     CDS             11104..11505
                     /locus_tag="PW004_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDL2726734.1"
                     /translation="MSRQLVLISHGKFCEELKKSTEMIMGPQEDIYTLPLLPEDGTET
                     YKQKFLTVTDSLNDFVVLCDLLGGTPANIISKLIMEGQQITLYAGMNLPMVIEFINSQ
                     MIGSEPEFVRAGCESIVSVNELINGIAEEEE"
     gene            complement(11551..12501)
                     /gene="yidC"
                     /locus_tag="PW004_001763"
     CDS             complement(11551..12501)
                     /gene="yidC"
                     /locus_tag="PW004_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDL2726735.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVVIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
BASE COUNT         4049 a         2046 c         2772 g         3774 t
ORIGIN      
        1 ttagttagtt tccgactgat gcttttgata atttctggat cagtatgcat ctgacaattt
       61 tttcccgtta attgaagttt taccagcatt tcttggccaa tcatttctga aaatcctcgg
      121 gcaaagacaa aaacccggtc agcaaatttg attctttgta ttgcatcttc aatgttcccg
      181 atttccaaca tattgattgt acgtaccact tcttgttcat tttttagaat ggctcggcgg
      241 attcctttgt ctactttatc taaaaatgaa aaattgatgt tcttgttttc tccttctttt
      301 agatgatgct taaacgaagt aaacccttca tagcctttct ttttcattgt ccgcacaatg
      361 gtggctgttg agacatttgc ttgttcactt agtttcacga tggaatagtt agggatctct
      421 aggatgaggt gttcgataaa gttccagagg tactgctctg tttcacttaa tttgtgattc
      481 atattctttc cttctttcac caaaagttag ttaaaaaaag cacgacgagt ttataaatgt
      541 aaaggttttc tttgtgttgg gacgattaag aaaacatttt ctatttcgcg tatttcataa
      601 tagaatcaag gaggtcgttg gttatgtcta tctacacatg cacgatgaat ttggcaattg
      661 atctatttat tgaaacagat gagatgcatc cttttatggt aaaccgcaca aaagaggatg
      721 acattcaggc aaacggaaaa ggggtgaatg tgtcactagt attaaagatg ctagatttcc
      781 caagtacggc attaggtttt tctgctgggt tcacaggaaa gtatatagaa gactatttac
      841 agcagaaaca aatccaaaca gagtttattg aagtgcctgg tatgacgcgt atcaatgtat
      901 ttacacaagt caatcaaacg ggagaagaat acaaattagt gaatcaagga cctgaaatac
      961 cgcagacagc tgttcataat tttttaaatc aaataagagc attacaagca ggggattatc
     1021 tttgtgtttc tgggagttta ccaagagggc tgtctccaaa ggttttaata gaaatcagtc
     1081 ggatttgttt gattattgat agtagtgatc aagaagtcat ggattgttta ccctatcgac
     1141 cgtttttgct gaaaccaaat gaggaagaac tttcttcttg gttcggacga aaaatggtta
     1201 cagatgaaga ttactttgtt tatggacaac gactagtaga gtcaggcgca gaaaatgttt
     1261 tgttatccct cggtgagaaa ggagcgattc tttttacgaa agacagggtg attcgaggaa
     1321 actcgccaaa aggaaaagtt gtcaatacag catgtgcagg tgatgccatg ttaggcactt
     1381 ttttagcagg atacatgaat cgacgatcgc ttgatgaaac gttacgtaaa agcatagcag
     1441 caggcagctc tactgctttt cgtaaaggac tgactgattt tttggatgtg gaggaattaa
     1501 gtaaacaaat caaaatcaag gaagagaggt tcgaaagatg ggaaaatatc aactaattgc
     1561 agcaaccggt tgtccgacag gaattgcgca cacctacatg gcgcaagagg cattagagca
     1621 agcagctagg aaaaaaggca tcacgatcaa agtggagacc catggacaaa ttgggatcga
     1681 gaatgaatta acggctgaag aaatcaaaca ggcagatgct gtcattattg ccgcagacaa
     1741 agatgtccat tcagaaagat ttgtgggaaa acgagtcatt gatgtttctg tcagtacagg
     1801 aatcaaggat gcacaacggc tgattgacga agcattggca ggaaaaggta ctgtcttagc
     1861 agataatgaa gctgctgata ctttagaacc agaagcgata agtggtgcta attttggtcg
     1921 tagcatctat aaaaatttga tgaacggtgt ctctcatatg ttgcctttcg tggtagctgg
     1981 cggagtattg attgctattt catttgccgt ttggggaatc tattcttctg atccagatca
     2041 tgcacaatac aatgctacag cggcaatggt aaaagcagtt gacgatgcag caatgggaat
     2101 gatggttcca gttttagcag cttatattgc agaagggatc gcgaaacgtc cagggttagt
     2161 cgttggattt gttggtggaa tcgtagccat gaatggcggt actggatttt taggaggtat
     2221 tttagctggg ttcttagcag gttattttat cttatttttg caaaagctac tgataatttt
     2281 acctaaacaa ctagatggct tgaaggcaat cgtcttatat ccagttattg gtgtagcatt
     2341 tattggcatc gtgatgtctt tgctggcagg tccaatgaag gctataaatg aaggaatgat
     2401 gagcttttta gctggatttg aatattcgaa tccacttgtt cttggcgtga ttgttggctg
     2461 tatgtgtgcg tttgatatgg gaggaccggt taataaggcg gcatatgtaa caggaacagc
     2521 tcttttgaca caaggaaaca cgacttttat ggctggtgcg tcagcagctt gcattgcacc
     2581 accgttgatt actggttttg ctactttatt gtttggtaaa tattttgata ctaatgatcg
     2641 taatgcaggt ttggttaatt ttattttagg atcaactcat attacggaag gagctattcc
     2701 gtttgctgcg aaagacccga tccgagtatt accaacgatg atgttaggct catcaattgc
     2761 tgctgtcttg acctatctgt ttggtgtcca agtaccagcc ccacatggcg gttttttagt
     2821 gttacctgta gtgacgggaa aaatccaatg ggttacagcg attcttattg gttcgatcgt
     2881 tggtggagtg atactaggtt tgatccaaaa gaaaagagta actgataaag acaagaagag
     2941 ggataataaa gcaacaaatc gtacagttat cgaagaagga agagcaggag gtttatcatg
     3001 acaatcattc atgaagagaa cattttttta caacagccat tagataatca agaagaagca
     3061 tttaagtttt tagcaaagca aacattctgt ttaggaatcg ctcatgacga aaaagaagta
     3121 tatcaaaaac ttctggaaag agaaaaagaa ggtactacag gaataatgtc aggttttgct
     3181 atccctcatg cgaagtctca aacaatcaat gaacctagtt tgattatcgt cacactgaaa
     3241 aaaggaattg aatggaacag tttagataga cagtcagttg attttatttt tgcgttattt
     3301 atcccagatt cagaagcagg aacgactcat ttgaaattat tgtctctcgt tgcacgatca
     3361 ttgatgcgaa aagaggttac agaacagttg agagctgcaa aatcaaaaat agaaatagct
     3421 acaatattaa ctaaagaaat aggtgaagat atatgaagaa aatgagtata gaaaaacagg
     3481 aacgtttgaa gcgattgacg gatgaagctg gaattattag cgcgctagct atcgaccaac
     3541 gtggtgcttt gaagaaaatg atcaaccaat ataaagaagc tacatcagaa gatatcatcc
     3601 aatttaaaca gatggtttct cagtacctga ctccgtatgc ttccgctatt ttactcgatc
     3661 cagaatatgg tctgcctgca gcagcgcttc gagagaaaga atgtggtttg ttgctagctt
     3721 atgaaaaaac cggctatgat gcgaaaatgc caggccgact accagattta ctgaatagtt
     3781 ggtcagtgaa aaggttaaaa gaagtgggtg cggatgcttg taaattttta ttgtactatg
     3841 acgttgatga accagtagag attaatgtgc aaaaacaaat agttatagaa cggattggtt
     3901 cagaatgcga agcagaaggc atacctttct ttttagagct cctttcttac gatgcaacta
     3961 ttggcgatcc gtcttctctt gactatgcaa aagtaaaacc gcataaagtg attgaagcca
     4021 tgcgagaatt ttcaaaagaa cgttatcaag tggatgtatt gaaagtagaa gtaccagtga
     4081 atatgagatt tgtggaagga tatgggcaag atacttgtta tagtcaagca gaggcatccg
     4141 cgtatttcta tgaacaatcg aaagtgactg aacaaccgtt tattttttta agtgcgggag
     4201 tcagcgctgc actatttcaa gatacattgc gttttgcaaa acaatcaggt tcgacattca
     4261 atggtgtctt atgtgggcga gcaacatggg cgaagggagt agcacctttt gttaccgaag
     4321 gcgaagaaaa agcgagtagt tggatgttag aagaaggaaa acaaaatatt aaagaattaa
     4381 gtacactatt agaagaaaca gcaacttcta tttatgttaa caagtaagag aaaggattgt
     4441 ctgagtgaca gattcaaaaa ataattagtt atttaaaaaa atgtttgtca aattttatag
     4501 aaaaaaagta taaaactgca aaagttatct aaaactaata ataacatttg tcattaataa
     4561 gctattttta gttgttgtaa atgatattgg aagtaataaa acgatagttg taggaatatc
     4621 atagggatta atgtggatca cgctgctcga taagttagac cattttgacg gatcgcttat
     4681 gtggttcgtc tttttttctg ttcttttatt ggtaactggt atttttgagg tatctttgtc
     4741 tattcaagat ataaagcttt aaagagctgt tataatagct tttttggtct aaaaaacctt
     4801 tgacaaatga ttaataattc actattcttg tgttgtggtt ataaccagta aagaagaggt
     4861 gatttttatg gagagctcaa ataaaggaaa atcattgtat tatcaactag ttgatttatt
     4921 gaaggatcgt atcgaaacac agatgactcc gcatgataag ctgttatctg aaagagaact
     4981 ttgtcaacac tatggggtga gtcgtaccac cgtacgtctg gctttacagg aattggaaaa
     5041 tcaaggttat atttaccgaa gacatggaaa aggtacctat gtctctgatt tgtctgataa
     5101 gtcatcgaac ttagctggag catatagctt tacagaagaa atgcgcgaaa tagggcgcgt
     5161 ccctaagacg gtcattttag aatttagaca aatggaagct aataagagaa ttgctaagaa
     5221 tttgaacatc catttaggtg aaactgtatt taaacttaaa agattgcgtt tggcagatga
     5281 agagccattg atggttgagc gttcttatct accggcgaaa ctatttatgg gacttgattt
     5341 gccgttactt gaaagtaaac cgttgtatga tgtgttttca caggattatg aacaacaagt
     5401 tcaattagct gatgaagagt tttatgcaag tatcgtgcaa aaaaaagatg ctgttgtgct
     5461 acgaatcgct gatggcgctc ccgtcttaca tctgacgagg ataacctata acgtgaaaaa
     5521 tgagattatc gaatatacag atagtgtagc aagagcggac cagtttcatt ataaaattcg
     5581 gcatatccga aaatgattga gagggagaac aacatgtttg agctatcgaa gaacaaatta
     5641 aaacaaatag gtgcagcaat cacgacacaa gaaataaaac aacaaccaga tctctggaaa
     5701 gaaacaatcg atagattgat gcaaagccaa gaaaatttag ctctattttt tgatcaaata
     5761 aaaaaagaag caaacggaag aagaatacgt gttattttta cgggtgcagg aacgtctcaa
     5821 tatattgggg atacgctctt accctatcta aatgaacatg gagacaatca gttttttatc
     5881 ttccacagtt ttggcacgac agatattgta gcttcaccaa aagaattttt gtttccaaat
     5941 gaaccgactt tattagtttc atttgctcgc agtggcaaca gtccagaaag tgtagcggct
     6001 gtgcaaattg caaatgaaca agttgaaaca ttgtttcatt tgacgattac ttgtgctaaa
     6061 gaggggatgt tggcaaaaaa cgcacaggat gataaacaga attatctgtt attgatgcca
     6121 gagagagcaa acgatgcagg ttttgctatg acaggaagtt ttacgtgtat gttattgagt
     6181 gctttatgga tctttgatca aacagcaatg gagaaaaaag aaagatatct tgaacaatta
     6241 gttaaaatcg gtcaagatct tctttcaaga gatcaagaaa ttcaaaagtt attacctgat
     6301 ttttttgaac ggatcgctta tctgggttca ggaagtttgg caggtgcgac aagagaagca
     6361 caattaaaaa ttttagaatt aacagcaggt aaagttgcta caatttttga ttcttcgatg
     6421 gggtttcgtc atggaccaaa gtctttttta aatagcgaga cactcgttgt aggttttgtg
     6481 agcaatcaac catatacgcg aaattatgac ttagatattt tagaggaaat aaaagaagat
     6541 cgggtcactg ccaatgtcat tgcgattgcg caaccgggta ctattgattt ctcaggtatg
     6601 actgtgctat tggaagactc ggtgagtaaa ttgcccgatg cctatcttgc attagcgatg
     6661 attatgactg cacaaatcat tgcactctat acttcaatca aagtagctaa taaaccagat
     6721 acaccatctc caacaggcac ggtcaatcga gtagtcaagg gcgtcattat tcataagtat
     6781 gaaaccgaag gagaaatgac agatgaatga agaaaaaaat agggcaatgg agagactatc
     6841 aacaaaagat gggattatta gtgcattagc gatcgatcag cgtggtgctc taaaaaaaat
     6901 gatcaaatcg ttgggaaaag agccaagtga agaccaaatc agtgaattca aagaactagt
     6961 atcagctgag ctgacaccct atgcttctgc tattttattg gatccggaat atggattgcc
     7021 agcagcaaaa gtcagagcaa aagagacggg gttattgtta gcttatgaga aaaccggcta
     7081 tgacgcaact acgcctggtc gtctacctga tttattagcg gattggtctt ctctcagatt
     7141 gaaagaacag caagcggatg cagtaaaatt cttactttac tacgatgttg atgaagcacc
     7201 cgaaatcaat catttgaagc atgtgttcat cgaacgttta ggtagtgagt gcgaaggaga
     7261 aaatcgtccc ttctatttgg agttagtttc ttatgacgca caaataagcg atagttcttc
     7321 tccagagtat gcgaaagtca agccccacaa agttattgag atgatgaaag aattttctga
     7381 tccaaaatat aaagttgatg tgttgaaagt agaagtacca gtcaatatga attatgtgaa
     7441 tggctattcc acaggtgaat ttattcacag caaagaagag gctgctgcct tctttaagga
     7501 gcaaagtgaa gcgactccat taccattcat ttttttgagt gggggtgttt caacagaatt
     7561 atttcaacaa acactgattt ttgctcatga atcgggttcc accttcaatg gtgtattgtg
     7621 tggacgtgcg acatggaaag atggggtcaa agcatacgtc gaaaatggaa aagacggagc
     7681 tattcattgg ttaagaacaa atgggagaga aaacatcgag actttaaata aagtaataaa
     7741 tgaaaccgct accccttggt tcaataaaac aaaaagcaaa aaggtgaaga tgaaatgatt
     7801 acgacagtta ccttgaatcc ttccatcgat atcgcgtata caattgaaga attaaacctc
     7861 gacaaagtca atcgagttga aacagtcaat aaaacagcgg gaggaaaagg agtgaatgtg
     7921 gcgagggttc taaatttatt aggagaagag gtgatagcta ctggtttaat aggtggccag
     7981 cacggtgaat atctaaagaa ccagctagat cagttaggga tcatgcatca gtttgttcca
     8041 attaaaggag agacaaggga ttctattgct atcctgcata gttcgagaca aacagagctt
     8101 ttggaacgag gccccttcat aacaaagaaa gaacaacaga catttaccga tcatttcgag
     8161 cagctcctcg aaatatcaga tattatgacg atctcaggta gtttacccca aggatgccct
     8221 cttgattact ataagcaatt ggttgcttta gctgataaaa aagggaaaga catattacta
     8281 gatacttctg gtaaattttt gcttgaagta ttggaaggta aggagaagcc actacttatc
     8341 aagcccaatc tggaagaatt aggagaactg attggccaac gtatctgttc ttcgattcaa
     8401 ttagaaaaaa ttcagtcctt attagctcat ccaatatttg aaggagtgcc atgggttgtt
     8461 gtttctttag gagagagagg tgctgtggca aaagttgctc agtgttttta cgaagtcatt
     8521 attcctaaaa tatcagttgt taatcctgtt ggttcaggtg acgcgaccct tgcaggatcg
     8581 gcccatgcgt tatcaaaaag gcaatcaatc gaagagattc tcgcattttc tatggcttgt
     8641 ggtatggcaa acgcgcaaca agagaaaact ggctggatcg accctatgaa agttacgatg
     8701 ttgatgaaac acattcaagt taagaaaatc aaatgaactt aaatataaaa gaaagcgttt
     8761 tctttagttg ggaaggaatt tgttcttagc ttaaaaatga aatataagaa agaaaaaatg
     8821 aaaacgttat ctttgaaaca gaagggatat ttgatgatgt cacagtttta attgaacaat
     8881 aaatagaaaa aatagataag gagaatgcat gatggcaatt attggtgtaa gaatcgatgg
     8941 acgtttgata catggataag tagcaaatct ttggacaacg aaactgaata ttagtcgaat
     9001 catggtcgtg gatgatgagg tagccacaaa cacaattgaa aaaagtggat tgaagttagc
     9061 gactccagca ggggtaaaat taagcatttt gccaatcgaa aaagcagcaa caaatatttt
     9121 agctggcaaa tatgactctc aacgagtatt gatcgtagca aaaaagccag atcgaatctt
     9181 atctttagta gaaaaaggag ttcctatcaa agaagtaaat gtggggaata tgtcacaaac
     9241 gaatgaaaca cggtccatta caaaatcaat caatgtggta gatgcagata tcgaagtatt
     9301 taaaaaatta gatcagcttg gtatacattt aattgctcaa atggtaccaa acgataaagc
     9361 agaagacttt atgaatcttt taatcaaata ggaggaatga tccatggata ttcaatggtg
     9421 gcagatctta ttattaacat tatatgcagg ttatcaaatt ttagatgaat tacagatcta
     9481 ttcttcaatg agtgcgccag tgtttgcagg gttatatgca ggtttagtaa tgggtgattt
     9541 aaacgcaggt ttgattattg gtggcagtat gcagttgaca gttttaggag tggggacgtt
     9601 tggaggcgca tcaaaaatag atgccaactc tgggacgatt ttagcaactg ccttctcgat
     9661 ttctcttgga atgaatccag aacaagcgat tgctgctatt gcagtgcctg tagcaagctt
     9721 aatgatccaa ttggatattt tagctagatt cgctaacaca tactttgccc atcgtattga
     9781 ccgtcacgta gaagacatga attatcaagc aatcgaacgc aatttcttaa tgggagcttt
     9841 accttggtcg ctctctcgta tgattcctgt atttttagct ttagcatttg gtggtggatt
     9901 agttgaaaat gttgtttctg ttttaaacgg gaatctaaaa tggctaggcg atggactttc
     9961 agtagcagga gctgttttac cagctgttgg gtttgccatt cttctgcgct atttaccagt
    10021 aaaaaaacat ctatcttatc ttattttagg atttacgatc acggcattat tagcaacgtt
    10081 attctctaat gttcaattat taggaacaag tgtggcaagc gttgttaaag atcttccggc
    10141 aactttcaat tcgctgccga tgcttgctgt agcattgatt ggtctagcac tagcaatgat
    10201 cagttataag aatgatcaaa acaaagacca tcaaccatta gctagtgagc catcacattc
    10261 tgttgagggg gaaatcgaag atgacgaaat ctaattacaa attaacaaaa gaagatttta
    10321 agcaaatcaa tcgaagaagt ttgttcactt ttcaattagg ttggaactat gagagaatgc
    10381 agggttctgg ctatttatat actatcttgc cacaactaag aaaaatatat ggagacaata
    10441 caccagaatt aaaagagatg atgaaaacac atacgcaatt ttttaatact tctaattttt
    10501 ttaatacaat cattactggg attgatcttg caatggaaga aaaagaggga attgcttcaa
    10561 aggaaaccgt atcggggctg aaagctggat tgatgggttc ctttgcagcg attggagatt
    10621 ccgtatttgc tgctttgatt ccaacgattt ttggggcttt ggcggcgaat atggcaactg
    10681 atggaaatcc gataggtgtg tttatatgga tttttgctca gatcgttgtc atggtttttc
    10741 gttggaagca acttgaattt gcatacaaag aaggaatttc cttagttact actatgcaat
    10801 ctcgcttaac tgcattaacg gacgcagcca ctttattagg ggtattcatg gtaggggcat
    10861 tagttgcgac aatgatcaat gttaaatttt catgggcacc aacgattggc gatgtgacat
    10921 tgaatatgca aaactatatc gatatgatct tacctaaaat tttacctgct gggatcgtcg
    10981 gtggtgttta ttggttatta ggaaagaaaa acatgactcc tactcgagcg atttttatcg
    11041 tattaattgt gtgtgttgct ttatctgcat tgggtgtgat ttcgaaataa gggagagcga
    11101 aaaatgagta gacaattggt attgattagt cacggaaaat tttgtgagga attgaaaaaa
    11161 agtacagaaa tgatcatggg gcctcaagag gatatctaca cgttaccttt attaccagaa
    11221 gatggcactg aaacatacaa acaaaagttt ctcactgtca cagattcgtt aaatgatttt
    11281 gtggtactat gcgatctttt aggtggtaca cctgcaaata tcatttctaa actaataatg
    11341 gaaggacaac agattactct ttatgctggg atgaatcttc cgatggtcat tgaatttatc
    11401 aactcacaaa tgattggttc agagcccgag tttgttcgtg caggatgtga aagcattgtc
    11461 tctgtcaacg aattaattaa tggaatagca gaagaggaag agtaacaaaa aagatgaggg
    11521 cttagctctc atcttttttg tcctatgttt ttatttccgc ttctgctgct ttcctgcgtt
    11581 gcggcctttg ttgtttgtac tgttttttgg ttgtttgatt tgttttgttg cttgtttgtt
    11641 gttagctggc tttgctgatt ctgtcacgtc ttttcttgga gtgacgactt gttttggcgg
    11701 gttttgtttc aactcttctg ccacttgtgc tttcaaacgt ggtttcatca taacgtttgt
    11761 gatgaatgtt tgcagacagc tgaacacacc accgactacc cagtaaagtg taacaccggc
    11821 tggcgcactg atcgacatga agacgatcat agcaggagag acgattagca tcgtacgcat
    11881 cgtctttttc tgttcttcag gaataccgat cgttgagatg tatccttgaa gcagataggc
    11941 gacccctgca atgacaacaa gtaccatact tggtgagcct aaatcgatgc cgtagaaaga
    12001 tgattcccga ataccttctg tataacgtgc cgtaaagtac aaggcagaga agatcggcat
    12061 ttggatcaat agaggcagac agccgattcc gccggtcata ctcaatccgt tttcgcggta
    12121 tacttgctgc atttctgctt gtgctgccat ttgttcttca cgtgttgttg cagctttcaa
    12181 tttttgttgg gctgcttcaa cttgtggttt taacgcttgc attttttcag attggatcat
    12241 tgtttttttc gattgactaa tacctagtgg caagatgatg atacggacga tcacagtcat
    12301 tgcaataact gcccagccat aagaccaatt gaaattctca actagatatg taatcgcttg
    12361 gcccatagga tggacgagta ctcgataaac gattcccgaa gtgtcagggt ttccgtttga
    12421 atccatccgc acacagccag ataaaaagag caggatactg acaagtccgg atcccaataa
    12481 ccagtttttc catttattca tagaatactt cctcttctat aatttttaca caaacttaac
    12541 tatactaaaa aagcgcatgg aattcaatca acttcataga aatttcatat aaatattcgt
    12601 tcttttacgt agcgaaaaat tttgaaaaaa agacaaaaaa t
//
LOCUS       DANKKG010000075        12606 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000075 DANKKG010000000
VERSION     DANKKG010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12606
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.075"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(18..1394)
                     /locus_tag="PW004_001764"
     CDS             complement(18..1394)
                     /locus_tag="PW004_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDL2726736.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(1522..2640)
                     /locus_tag="PW004_001765"
     CDS             complement(1522..2640)
                     /locus_tag="PW004_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL2726737.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            2782..3216
                     /locus_tag="PW004_001766"
     CDS             2782..3216
                     /locus_tag="PW004_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2726738.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(3294..3827)
                     /locus_tag="PW004_001767"
     CDS             complement(3294..3827)
                     /locus_tag="PW004_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HDL2726739.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(3921..5099)
                     /gene="mutY"
                     /locus_tag="PW004_001768"
     CDS             complement(3921..5099)
                     /gene="mutY"
                     /locus_tag="PW004_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HDL2726740.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(5092..5889)
                     /gene="recX"
                     /locus_tag="PW004_001769"
     CDS             complement(5092..5889)
                     /gene="recX"
                     /locus_tag="PW004_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HDL2726741.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            5996..7378
                     /gene="rlmD"
                     /locus_tag="PW004_001770"
     CDS             5996..7378
                     /gene="rlmD"
                     /locus_tag="PW004_001770"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDL2726742.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(7552..8130)
                     /locus_tag="PW004_001771"
     CDS             complement(7552..8130)
                     /locus_tag="PW004_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HDL2726743.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            8452..8922
                     /locus_tag="PW004_001772"
     CDS             8452..8922
                     /locus_tag="PW004_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HDL2726744.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(9030..10256)
                     /locus_tag="PW004_001773"
     CDS             complement(9030..10256)
                     /locus_tag="PW004_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDL2726745.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(11010..11210)
                     /locus_tag="PW004_001774"
     CDS             complement(11010..11210)
                     /locus_tag="PW004_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL2726746.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(11344..11952)
                     /locus_tag="PW004_001775"
     CDS             complement(11344..11952)
                     /locus_tag="PW004_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HDL2726747.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(12037..12507)
                     /locus_tag="PW004_001776"
     CDS             complement(12037..12507)
                     /locus_tag="PW004_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HDL2726748.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
BASE COUNT         3801 a         2521 c         2076 g         4208 t
ORIGIN      
        1 cataaccaag tttccaatta tcttcttggc tcgtcatctt cgaattcttt tctgaagtat
       61 cttcgttctt cttcaggttc cgcttctgtt tcttccggat catgaaaaat gacacggatt
      121 ttaaggattc gagaaccttc catttcttca gaaaccagcg taatattatc gacatcaaac
      181 gataactttt cgccttcatc cggaatcatg cctaatgctg taatcaaata accagccatc
      241 gtatctacat cactcatatg cagatcactg tcaaaagctt cattgaactc atcaatcagc
      301 atacgtcctt ggatgatata ttcgtgttca tcgatttttt cataaagatt ctcaacttca
      361 tctgattcat catcgatttc tccgacgatt tcttctaaaa ggtcttctag tgttgctaac
      421 ccaacgacgc cgccatattc atctaataaa atagccatct gattctgcgt ctttttcatt
      481 tcatataaca gatcatcaat aaagaccgtt tcaggaacga ataaaggctc ctgcatgatt
      541 ttcttgatat ctaaattatc aaagccgaat ttatgggcag cttttagtaa attctttgta
      601 tgaaggattc cgactacttt atctttgtct tcgttataaa cagggattct tgaatagttt
      661 tcggataaaa cttcattcac attttcttcc acagtgtcgt tgatatcaat cataaatgca
      721 tccgttcgtg ggaccatgac ttctcgggca actttcgtat caagagaaaa aaccccttgg
      781 agcatttcta attcttcatt attcaatacg ccttcgtttt caagcatata acgcatttca
      841 tctctggtca tttttgaatc ttcatcatca aaagtcatcg gcgtgatacg cgccaataaa
      901 cttgtggaag cagaaagcag ccagacgaag ggcttagcta ctacaccaat caagcgtatc
      961 agccctgaag tgaactttgc gacttcttct gatttattta aagcaatacg tttagggtac
     1021 aattctccaa aaacaatcga tacataggtc aaaatagcca gcacaatgat atttgcaata
     1081 ttttttgccg ctgtgccgcc acctaatacc ggagctaacc gttccgataa agtattagct
     1141 agtgaagcac cagacaaaat gttgaccaaa gtgattccta cttggattgt cgataggaaa
     1201 ttatttggat tctgcaatat ttttaaaagc ttttgagatt tcacatctcc ttcatcagct
     1261 ttttgctcga ttcgattctt attgatcgaa acaacagcaa tttcggaagc tgctaaaaaa
     1321 gcattgatca atgtcaaaac gatcaataat aaaatctgcg ctaacagcga ctgactctca
     1381 gggtcagcat tcataatcgc attctctcct tataaaatta atttttagtt tacgatagaa
     1441 aaaaacgtgg cactatagcc acgttaaaat ggtatcacaa agcaattttc tttgcaataa
     1501 aaacaagtgt actaaaacta cctacgaggt cgtagcaatt ttcttcttgt tttcattttg
     1561 cttgtcttct ttgacgattt tgattatata ggaaatgatc gtcttgcaga ttgcgtaaaa
     1621 aggaacacct aagaagatac caagcaatcc ggcaatattt ccagctacta gaagaacgat
     1681 aatgatcgtt aatggatgga ttttcagtga tttcccaatt acattaggat aaatgatatt
     1741 cccatccaac tgttggacga ttagcacaac cacacaacaa agtaatgctt ttaccggatc
     1801 attgaagact gttactagaa atgcaggtgc tagtcctaga taaggtccta aatatggaat
     1861 taggtttgtc aatcccgcaa tgaccccaaa taagaaagca taatccacac caattaccat
     1921 atatcctaaa aatgtgaagg taccaacgaa taaacattcg attgcttgtc cgctaatgta
     1981 attagccaat gttttattca gttgccccaa taactccaca atttggtttc ttcttttttc
     2041 tgggaaaaac tgttggatgt ttggcactag tttttctccg tctttcaaca tatagaacaa
     2101 gatgaatggt gctgtaatga ttactacggt tgccgaagcg atcgttccaa cgatcgaacc
     2161 taaactattg gatactccac taaggaattg ctgaatgatt gtcccgtaag aaatgtctaa
     2221 ttgctcaaaa tatttattga tatctacttc cttgaacaaa ggcaattcgg ctacctcttt
     2281 taaccagctt tctacctgtt tgatgaaatc aggcatatta gatgccaatg aggaaatttg
     2341 ctgtactaga cttgggatga cactaagtaa aatccaaata attgctgcaa ctaaaaggag
     2401 cagcacaatt gcgacagccc atattctttt gatttttgct ttcatcaaca gcttgacaat
     2461 aggattcaat aaatagtaaa gaaagcctgc aatcagtaca ggtgcaaata acgtggaaaa
     2521 aaatgtcccg atcggttcga aaataaagtt tatttctgat gaaatcatga tcaatgcagc
     2581 tatgatcaat aattcaagcg accaaaacat tagtttagac tgtctaagtt tttcaaacaa
     2641 tagatttcct cctttctttc ttactagtat atattatatt cgttaaaaat tcttttattc
     2701 tttttttatt tttgaaaact tgccatactt tttatttgtt ttattttcca gtatactata
     2761 cgaaaggaag gtggctagac catgaaagtt gtacaaacaa aagatacaat gagcaatatt
     2821 tacttagatg ctgtacggat caggcatcaa gtttttgtag tcgaacaggg tgtgcctttg
     2881 tctcgagaaa tagacaaaga tgaagcccat tgtatccact tcgtcctgta ctcagataaa
     2941 aaagaaccgc aaggaactgt acgtcttttg cctttagaaa acggaaaaat gaaattacag
     3001 cggatggcga ttctttctga atacagacac caagggttag gaaaaatatt gattgaagaa
     3061 gcagaaaact ttgctaagaa tcaaggctat aatacgattt tattaggggc acagtccaca
     3121 gctgaaactt tttacgaaaa actaggatat acagcttacg gcgatccttt tgaagatgct
     3181 ggcatgcctc atatttatat gaaaaagact ttataaaaat ttatttcgaa aaaagcaaaa
     3241 aacagcaagc ttttgagaaa aatctctcac tggcttactg ttcttttttg tgtttactta
     3301 cgcgacaatt gcttgtaacg gttataccaa atatcaatat agccttccga gaacgggcca
     3361 tctccattat tgatccagtc aaccagaatc ttcacatttt cttttaggat gaagtcaata
     3421 tcgttcgggt aattcatcat tttgctgtgc atctcatatt catcgacatc caatagtcgt
     3481 ttctcgccgt caggaaatac cttgatatcc aagtcatagt cgatatattt caaggcctca
     3541 tcgtccagca aaaaaggtga agctagatta caataatagg aaaccccctt ttctctgatc
     3601 attgctatta cattgaacca gtatttttta tgaaaataaa caatcgctgg ttcgcgcgtt
     3661 acccatcggc gtccatcaga ttcagtgact aatgtgtgat cattcacgcc gatcatagag
     3721 tgttcgcttg tttttaatac catggtatct cgccacgttc gatgcaattt gccgtcgtgc
     3781 ttgtaacttt ggatcgtcac aaactctcct tcttttggaa ctcccatagc aacccttcct
     3841 taagcagcat tttgtgcttt gtgtttgggt gttcattaca ccgacatcat aattattata
     3901 tcaataaaaa tgagctttgc ctagaaagga ttcctattgt ttcgattttt ctctaactga
     3961 tctaccagtt tcatttggac cttaggaaaa acgaccgaat caaatgcatc aggcttcaac
     4021 cacttcgaag ttttgttctc ggtaaattct tgttctgctc cctcggttgc ccgtccgtaa
     4081 aaaaggagaa catgccattt cagatgacta aaaatatgag taacttctcc taaatgtcgt
     4141 gtctgccaaa caaagagttc ctgcttttca aatgggagat tttgctcttc ttctgctact
     4201 aggtcatcaa acagatcgat ttctggcttt gtttcccatt cttttttcaa tcgctcatac
     4261 tcctcttgtg tcacttccat cattggaaat gtccacatat tcgccagcaa tttttggcta
     4321 tctctttctt caaaataata cgctccagaa tgattttgaa gagctgcact gatataataa
     4381 acgtcttttg gctttgcttt ttttgttttt acaggaaaac tcgtttggat tcctcgtttg
     4441 ttagctaaac aaaaggcttg gatcggacaa gtctcacact ttggagaagt tggtgtacag
     4501 atagctgaac ctagatccat cattgcttgg ttgaattcac ctggatgctt ttcatcaatg
     4561 attttccgca tcgcttcatc aaagattttc ctgctagaag cttttgcgat gtctgcttca
     4621 atgcaaaaca atctgctgac tactcgcatc acgttgccgt caactgcagg ttctggaagt
     4681 ccaaatgcga tacttgcaat cgcgcctgtc gtatatggtc ctattccctt caatgagcta
     4741 atttcttcag gcgtttgcgg catttcccca tcaaattctg acatgatctg tttagcagca
     4801 gcttggatgt tccgggctct tgagtaataa ccaaggccct cccatgcttt taaaagcttt
     4861 tcttctggag catttgctag ctcttcaatt gtaggaaacc attccatgaa acgataaaaa
     4921 taatcgatca ctgtgtctac tctggtttgc tgaagcatga tctcagagat ccagatacga
     4981 taaggatcac gattatagcg ccaaggcagg tttctttttt cttgttcata ccattgaatg
     5041 aactgatctt gaaattcttt tgtttcttta tctgtccatt tttcccattc tttattcact
     5101 ttcctcttcc ttttccgtaa tgaatcgctg gatcatatcc aaatcaaatc ctttttgata
     5161 taagctttgc tttactttta gatttctttt tttaggttca aaccttaaat ttcttcgcca
     5221 tagtttatct ccttgtttga ctaaataatc atattctatt tcttgatctg cttcttgcgg
     5281 aagatcagct atcgcttgtt ggatcacttc ttgagaaaag cctttcgtca tcaatgtttg
     5341 ttggattttt ctcaataatt ctttttggct ttttccgtga ttttttccaa acgctttttc
     5401 ggctgtctga tgtgcatttt gaacttggtc ttcaaatgta tattggctga gcgcttgttc
     5461 aatgatttca ggtttcaaac ctttttgctg catctgctgc gctaatttct ttggtccttt
     5521 gtcacttaag cgcatattcg tgcgcacata actttcccca tagaccaaat catcgacaag
     5581 gtccaattct tttaagcggg ctacgatttt atttcgatct tcttgaagga tttccttttc
     5641 ttttaagtat gttcggattt ctttttccga tcgcaactga taacttaagt aattcattgc
     5701 taggtgaaaa ccgaaatcag ctccgctact cttcttgatc tcttggatag tttcttcgtc
     5761 taattcccgt ccttttaata atcgatagcg tactaataca tcttcagata cttgtaaagc
     5821 ttctccttca gataaatcta cgcgatagaa agggcctttg cctttactga ttctcgtaac
     5881 ggttaacata aacatcctcc tcgaacatat attctctatc ataccacaga aaatccccgt
     5941 aatcaggtat tatatgttag aataaaccac agaaaaaaga aaaagggaga tacttatggc
     6001 agaaacaatt gtcaaaactg gacaaacggt tcctttgaaa atcaagcgtt taggcataaa
     6061 tggtgaagga atcggttatt tcaaacggtt gatcatattt gttccatatg cactaccaaa
     6121 agaagaagta ctagtgaaaa tcacgaaagc aactcctcgc tatgcagaag gagaattaat
     6181 aaaagtcaaa aaaacaagca aagatcgcgt agtggcacca tgtcctgttt actatgaatg
     6241 cggtggctgt caattacaac acttagctta tcaagctcaa ttggatttta aaaaggatct
     6301 gcttttgcaa gcattggaaa aattcaaacc agcaggcttt cgatcttatc aacttttgcc
     6361 gacgatcggg atggatgagc cttggcatta tagaaacaaa gcacaatttc agttacgaaa
     6421 aaataagcag actcaaaaaa ttgaagctgg tttatacgaa gcaaactctc atcagttagt
     6481 ccctataaca gactgcttgg ttcaagaacc tcgaacacaa aaagtaatga atactgttgt
     6541 tcaactattg aataaatttg atgcacccat ctatgatgaa cggacaaaca gtggaatctt
     6601 ccgcacaatc atggttagaa tcggtgttaa aactggggag ttacaagttg tttttattac
     6661 tcgcagtaaa aaattcccgc aaaaaaacgc aatgatccgt gagatcaatg aacgactgcc
     6721 cgaagttgtt tcaatcatgc aaaatgtcca ggcacaaaaa acgtctctcg tgatggggga
     6781 tgaaacgatc cacttatggg gaaaagaagc cattgaagaa caaatcaatg aagtgacttt
     6841 cgatctttct cctcgtgcct tctttcaact caatcctcag cagaccgaat tgctttatgg
     6901 cgaaggaatc aaagcattag atgtccagcc taatgaaacg atcgtggatg cttattgcgg
     6961 agttgggacg atcggcttaa gtgtagcaaa acaagccaaa gaagtacggg gaatggatac
     7021 cgttcctcaa gcaatcgctg atgcaaaaaa aaatgcggaa agattaggat atcacaacac
     7081 tcgatatgaa gtcgggacag cagagcaagt tctgcctaaa tggttgaatg aaggttttca
     7141 gcctgatggt atcatcgtag atccaccacg tacgggtttg gataacgcat tgatccaatc
     7201 attattgaaa taccctccaa aaaaacttgt ttatatctcc tgtaatgttt ctacacttgc
     7261 cagagatctg gttaatttaa gtaaaaagtt caagattgaa tatttacagt ccgtcgacat
     7321 gtttcctcag actgcacgtt gtgaagttgt cgtgaagatg acgagactcg ctgactaaat
     7381 gaaagaggtt gtgaaaaagt ctgccctgta tcaagtaata agaacagcca aacaaaaata
     7441 gctcttcatg ttttatgaat actgtacttg tgaccgaaga gttaacagga ggacactatt
     7501 tatttaaaaa aagggttgtg acatagtttt tgtcacagcc ctttttgatt attattggat
     7561 attcacgtct atcacttgat aaaatgcatt tgtcgtatcg ctgatattcc atactgctaa
     7621 aatcacatta tatccttttc gatcctgcgg aagtgtgatc gtttggttga ctgtttttgg
     7681 tggaacagtt gctttgtcgt ctatttttgt gatcaattca aaatcactaa atttcaaagg
     7741 ttgattagga ttccaagttg gtttggtaat gtagtaatcc caagtacttg tccgatgttg
     7801 tgctgttaaa ttccaagtaa tatttaatgt tcctggatta accatgtttt tatgccatct
     7861 gttagatgtc tgttcatcta aaggctcaaa gccgctgact cctgcactag caatttttcc
     7921 atcaatgaat gtatttttgg tagcttcgat actttgcggt tcccattgtg ctcttccgac
     7981 attttgattt aagtttcctg ctgctgatgt tcctaggtat gcacggcttc ctggagaagt
     8041 aatataacca tgcgcaaaac ctggaatgct aaaaccgaac aaccctaaac taactaaccc
     8101 aataaacaat aacgtactaa tctttttcat gtagaaacct ccttcatttg ataaccacat
     8161 tgtaaacgct ttattgtatt aacacaaaca ttatgctttt ataattttat gttgaagtaa
     8221 aaaaggtgaa aaaagtaaga tgcagattat aacagaagta gacgagaaaa gtacaaatag
     8281 aaaacatgag aatcaaatca tatgatcttt ttcaaaaaca tcattcatat aaacaaatgt
     8341 tcaacttttt aataacaaca attatcaatt tttttcttac ttcacaaact aaatacaggt
     8401 cttgctgtga gccttcatgg taaactaata ttatagaaag gagttagtaa aatgaagatg
     8461 ataaaagata ttcgtttttg ggtttgtgtt ataggaattg tcatattagg ctttttatca
     8521 gggttgctta gcggaaatcc cggaaaatat tactattctt tgcaacttcc accatttgca
     8581 ccacctagct ggatatttgg gccgatgtgg accttactgt atatattgat gggtatttct
     8641 ctttatttgt tacttaatca caacaacaaa aaacagcgaa acaatcttgt cggtcttttt
     8701 gtgattcagt tcatttttaa ttttatatgg tctgctctat tttttaattt aagaaatatt
     8761 ttcattgcag caatcgatat tacactttta gttattttct tgtcagtgct tatgtatcag
     8821 ttgtggctgc atcatcgcct ggcaatgtgg ctcatgattc cttattattt atgggtacta
     8881 ttcgcaactc tattgaatta ttcaatttat tttttaaact aagcgctaaa aagagacgtc
     8941 ctgtaattta acacatagaa aaagtgccac ctaagttaga ttttttgtct aatttaggtg
     9001 gcactcttta ttaaccaaac tatttttttc tagtacccac ttgttgttcc atactcatta
     9061 ttctggtcat aaccataacc attttgttcc tgtccagcgt aaggatcata actttgagta
     9121 tcttgacttg gtgtatagta ctgacttgta tcttctgttt gctgataata ggtattcgga
     9181 tcttggtact gatcatatgt gttttctgaa taggaagaat aatcggtgta cccttcacta
     9241 ttcgatccat caccgtttgt cgaatcgcca tcttccgaat catcaaagaa catcattgaa
     9301 gcattctcat ttttcaaatt aggtaatgta tcgcttgttt ttatatttaa ttgtttcttc
     9361 agttcatttt ggatcgaaag aagatcattt tttcctaaaa tttgatagta gatatcattg
     9421 atcatttgtc cttcgccttg caactggtct tgtttgattg tttcaaacgc cggggtgtag
     9481 ttcgtcgcta catcaagcat gtcatcccaa tctaagtccg tcgtcacatt gttctcaact
     9541 gcttttaaaa tttttctata attgcttacg ccatctaaac ttaaaacttt attgacgatt
     9601 ttcgtcacta cttcccgctg acgacgttgt cgaccgatat cgccttctgg atcttcgtgt
     9661 cgcattcgag aataggctaa acctttttct ccattcaatg tttgttcacc tactggtaca
     9721 tggacaccat ccaaagtaaa gtcaatttta ttattcactt tgatgccacc gaccgcattg
     9781 atcaaatctt ccatgccatc caaattgatc gttacataat gatcaatagg aatatcgagc
     9841 agttgctcaa ttgaatccat ggccatttcg acaccaccaa atgcgtaggc atgatttaat
     9901 ttatctaccg ttccataacc tacgatattc gtataaatat ctcggtccaa actgatgatc
     9961 gttgattttt tttgtttcgg attaactgtt actaccatca tcgtatcaga acgtccttgt
    10021 tccgttcttc ctaatccacc ggtatctaaa cctaatagca aaaccgaaaa aggttctttg
    10081 tcatcaatgc ttactctctc agaacgtttg gaggatattc gattagagtt tttagaaatt
    10141 ttgtttactg tttggctcgc ttctcccata aatctgattc cgtatgctga taatccggca
    10201 atcagcaaaa cgaatacccc taacaaggtt aatagaactt tttgccatcg cttcataata
    10261 cacctctgtt tcacattttc taaaatattg tctcatataa ctgttcaatc agcaaattca
    10321 ctttttaatc ataagaaaac tttaaaaaac atttttgtgt aacgattatt attttttaaa
    10381 atttctcaaa attcttaact atcataccat accgtgaaaa agatcgaaag accgaataca
    10441 cgcagttcgc aatcgtttta tcttttttta tgtatttttt ccagaaaaag cgaacaacaa
    10501 ggtattttcc aaataatcgt gcagtaaaat gatcgcacat acctttccca tctttttaac
    10561 agtctttgcc taccgttcag attatcattc gatttcacga caatttcttc ctatgactgg
    10621 tccttttgtt aaacccatct tctattcgtt ttatttccag ctcactgcat ggctcttaaa
    10681 atgactcgaa ctagtgattg cattaccatc atcttttaat ggatagatat gctcctcttc
    10741 aaagtaaaac gattttcttt taaagtaata gaattgacat ggtaatgagt atgtatacta
    10801 gtggtttcac aaatggtctt ttattcaaca tccaaaaact gaaatttctt taaaatccat
    10861 tactaacgaa gaaaatgttc cttgcttgat aattagttag gacatgtcaa gtgaacaaat
    10921 gcatttactt agccaaaaaa tctaaaaact tcataaatag acaaaaagac acgactctct
    10981 aagaagtcgt gtctttattc aacaaattat tataatttag tcacttcagc agcttgaggt
    11041 ccgcgtgcac cttcaacaat cgtgaattct acttcttgac cttcttctag gctcttgaag
    11101 ccgtctcctt gaattgctga gaaatgaaca aatacgtcat ctccgccgtc tactgtaata
    11161 aacccaaatc ctttttcatt gttgaaccat ttaaccgttc cgtgttccat aaatgtatat
    11221 cctccaaaaa agttcttgca agtaaccttg caagaacatt atacccaatt tagaaaacgc
    11281 tagcaagctt tcgctctttt attttccaaa ttatttttaa gactgatatg acggaggaaa
    11341 tggttatttt gctgcttcga aacgtttgtt tacttcatcc caattaataa cattccagaa
    11401 tgctgcaata taatctggtc ggacattttt atatttcaaa taataagcat gttcccaaac
    11461 gtccaaacct agaattggtg tctttccttc catcaatgga gaatcttgat ttgcagtaga
    11521 ggtaatagct aattttccat tttccattac aagccaagcc catccagaac caaatcgtcc
    11581 agcggctgct tttttgaatt cttctttaaa agaagaaaaa tcaccaaaag cttcattaat
    11641 cgcttctttt atttctcctg taggttcgcc accagcattt ggtgccatga tttcccagaa
    11701 aaatgaatgg ttagcatgtc cgccaccatt gttacgtaca gctgtcttga tatctgttgg
    11761 aatagcgtcc atatcagata ataattcttc tattgttttt tcgcctaatt ctgggtattt
    11821 ctcaattgct gaatttaaat tcgtcacata agtattgtga tgcttatcat gatgcagatg
    11881 catcgtttct tcgtcaatat aaggttccaa tgcgtcatat gcataaggta gatctggtaa
    11941 tgtgtaagtc ataaaaaaat tcctcctcaa tgttttctta ataatgtcta cattacaaag
    12001 agtaacaaag agaaggaaat tcttcaattg ttatgcctag tcgtcttttt cactttgacc
    12061 tacttcttct tcaaaaacct cagtttcttt aaaaataaac aacttgctga aaatatagtt
    12121 cgcaaggata acaataattt gagtgatgat tttcgccaca tactcattgc tgttcataac
    12181 attgatcaac aataccattg cccccatgtc caatccgtaa gaaaggatac ggtagaagaa
    12241 gaacttaccg cattcaataa gtgcatgtgt aaaattcggt gattttgaat ggaataccca
    12301 aagtttgttc gttacaaaag caaacaatac agaacaaaac cacgaaatag aattggataa
    12361 ggccatactt aaatcaaaat attggcgtgc gagagcaaaa ctaacaaaat ttacaatcgt
    12421 agccaaacca ccaaaaaaga gatagactag tatctcccaa tatccttttg cttctaaata
    12481 tccttttaac tgtcgatact gcttcatgag ccaacctacc ttattaaaaa ttcttattta
    12541 tcatactcta tatttcaata actttcatct ttatttaaaa aaattaattt tttttgatcc
    12601 tttgtc
//
LOCUS       DANKKG010000076        12588 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000076 DANKKG010000000
VERSION     DANKKG010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.076"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(62..1093)
                     /gene="queA"
                     /locus_tag="PW004_001777"
     CDS             complement(62..1093)
                     /gene="queA"
                     /locus_tag="PW004_001777"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HDL2726749.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            1286..2461
                     /locus_tag="PW004_001778"
     CDS             1286..2461
                     /locus_tag="PW004_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HDL2726750.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            2465..3106
                     /locus_tag="PW004_001779"
     CDS             2465..3106
                     /locus_tag="PW004_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2726751.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            3103..4020
                     /locus_tag="PW004_001780"
     CDS             3103..4020
                     /locus_tag="PW004_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2726752.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            4017..4688
                     /locus_tag="PW004_001781"
     CDS             4017..4688
                     /locus_tag="PW004_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2726753.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(5229..5717)
                     /locus_tag="PW004_001782"
     CDS             complement(5229..5717)
                     /locus_tag="PW004_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HDL2726754.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(5918..6184)
                     /locus_tag="PW004_001783"
     CDS             complement(5918..6184)
                     /locus_tag="PW004_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HDL2726755.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(6184..6447)
                     /locus_tag="PW004_001784"
     CDS             complement(6184..6447)
                     /locus_tag="PW004_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDL2726756.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            6923..7108
                     /locus_tag="PW004_001785"
     CDS             6923..7108
                     /locus_tag="PW004_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL2726757.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(7250..9073)
                     /locus_tag="PW004_001786"
     CDS             complement(7250..9073)
                     /locus_tag="PW004_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HDL2726758.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(9671..12376)
                     /locus_tag="PW004_001787"
     CDS             complement(9671..12376)
                     /locus_tag="PW004_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HDL2726759.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VACMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
BASE COUNT         3620 a         2616 c         2378 g         3974 t
ORIGIN      
        1 ctttgtcata aaatctttgt aaagcttttt tgttattcaa aactagcggc ttttcataac
       61 cttatttcac gaacatcgca tctccaaaac taaagaaacg atagcgctct tcaatcgcat
      121 gctggtaagc agataaagtc aattctcttc ctgcaaaggc actaactaac atgactaaag
      181 tcgattttgg caaatggaaa ttagttgaga aagcttctac cactttgaat tcatagccag
      241 gcgtaatgaa aatatcagtc catccgctgt ctgctttgat ttctccatta aatttcgttc
      301 caatcgtttc taatgttcga attgaagttg ttccgacagc cacgattttc ccgccatttt
      361 tacgtacttg gttcaatcgc tcagccgctt cttccgtcaa acggtagaat tcactatgca
      421 tatggtgttc ttcaatttta tctacgctga caggtcggaa tgtacctaac cctacatgta
      481 gtgtcagata gactaattca acaccctttg cttcgatctc atcaagtaat tctttcgtaa
      541 aatgcaatcc agctgttggc gctgcagcag aaccattttc tttcgcatag acagtttgat
      601 agcgatctgg gtcttccaga cgttctttga tatatggagg caatggcatt tctcccaatg
      661 attcaagaat ctctagaaaa attccttcat actcaaagtc aatgatccgt ccgccgtgtt
      721 ccaattcttc tttcactact gcttgcaatc tgccatcacc aaaagagatt cttgtcccaa
      781 ctttggcacg tttcgcaggt ttgatcaatg tctcccacgt atctccttgt gtattcgtta
      841 gaagaaggac ttctaaatgc gcaccagtat ctggtttttc tccatataaa cgagcaggca
      901 gaactcgtgt attattcata actagcgcat caccaggctg taactcttca atgatttcat
      961 ggaaatgttt atcttccatc tctccagttt tccgatccaa aacaagtaaa cgagaatgat
     1021 cgcggtcttt taatggtgtt tgtgcgatta attcttccgg caagtcaaaa tcaaaatctt
     1081 ctgttgttaa catatcgtca ctcactttct atcatgtgct tcatcattct atcatgtttt
     1141 tcgttctgtt ggcaaaatca gcactttttc acttctattt tttgttttcc taaataaaaa
     1201 aactacccta tttgtatttt ttcattgttt taggcatact agtaatagat agttcttcat
     1261 tgaaaataga aaaagaggtg gcaccatgat tgaatttcaa aatgtgtcaa aaatttataa
     1321 aggtggaaaa gtcgctgtag aggacgtcaa tctttcattt gaaaagggtg agttcatttg
     1381 ttttatcggt acgagcggaa gcggaaaaac gacgacgatg cggatgatca accgaatgac
     1441 tgatccaaca caagggaaaa ttttgattga tggaaaatcc atccaagaaa ttgatccagt
     1501 ggaattgcga cgtcagatcg gctatgttat ccaaagtatt ggattgatgc ctcacatgac
     1561 gatccgtgaa aatatcactc ttgtccaaaa attgttgaaa gtcagtcaag aagaacggaa
     1621 taaaacagct gaaaaaatga ttgatcttgt ggaattgccc cgtgagatgt tagatcgcta
     1681 cccacatgaa ttatctggtg gtcagcagca gcgtattgga gtcgttcgtg cccttgcagc
     1741 gaatcaagat atcatcttga tggacgagcc attcggtgcg cttgatccaa tcactcgtga
     1801 ttccttgcaa gaccttgtca aagatttaca agagcgtttg ggaaaaacga tcgtatttgt
     1861 aacccatgac atggatgaag cgattaaact agcaagccgt atcgccatca tgagtgaagg
     1921 ccgtgtgatc caatttgata caccagaaaa tattttacgt catcctgcaa acgatttcgt
     1981 agaagagttg attggagaag atcgcctact acaagccaag ccaaatgcga caagagtcgg
     2041 cgaagttatg ctaaatacag caatcacgat tacaccggaa aaatcattac aagaagcaat
     2101 caaactgatg agggaaaaac gtgtagatac gttgttagtt accgataatt cgaatgtatt
     2161 aaaaggattt atcgatgtag aaacgttgaa taaaaaacga ggaaaagtat ccagtgtcgg
     2221 cgacattttg aataaacatg ttttttacgt aaaagaatcc gcctatcttc gtgatacctt
     2281 gcaacgcatt ttaaaacgcg ggctgaaata cgtgccggtc gttgatgaac agaacaaagt
     2341 cgtaggtatc ctgactcgtg catcattagt ggatatcgtc tacgatgtga tttggggaga
     2401 agatcccgca actactgatg ttgcagagtc agctactcct gaagaatcta aggaggtctg
     2461 acctatgcag cagttttttc atcagcacgg ttcagaaatt ctctcaaaag gttttgaaca
     2521 tctttatatt tcaggacttg ctttgatcat tggtatcatc attgctgtac cgcttggtgt
     2581 cttactgaca cgttttccaa aagctgccac gattgtgatt ggcttaacca gtgccttgca
     2641 gacaatccct tctcttgctt tattggcatt gatgatcccg ctatttggcg taggtaaatt
     2701 acctgcgatt atcgcactct ttatttattc attattgccg attttacgta atacgtacat
     2761 tgggatgaaa aatgtcgatt ggaattatcg ggatgtggca aaaggcatgg ggatgactga
     2821 gttccaatcc attttttccg ttgaattgcc cattgctatg ccaacgatca tggctggtat
     2881 tcgtttagct gccgtttacg tgattgcatg ggctacactt gcctcttata tcggcgctgg
     2941 tggtttaggc gatttgattt ttagtggact gaataattac caaccagatc tcattttggc
     3001 aggtacgatc cctgtaacaa ttttagcact attggctgat tggcttctag gtctcttgga
     3061 acaccggctg acaccgatcg cacttagaga ggagaatgaa gaatgaaacg tatactaaaa
     3121 atgaccatgg ttcttttctc tcttcttata cttgcgagtt gttccttccc tggtcttgcc
     3181 agcaatacag atgaagacac catttcgatc acaggtggaa tcacttcaga agcacagatc
     3241 ttagcaagtc tagttgccgg aatggttgaa cattatacag ataaaaatac cgcaatcatc
     3301 aacaatctgg ctactacaac tatcaaccat caagcgatga tgaacggaga tgcttcaatt
     3361 tcagcggctc gctacacagg aacagattta acgactacat tgaaccttcc tcctgaaaaa
     3421 gatccgaaaa aagcgtttgc taccgtgaaa gatgaatttg agaaaagata cggtcaaacg
     3481 tggttcccgt cctatggttt tgaaaatacg tatgtctttc ttgttcgaaa agacaccgca
     3541 caaaaatacc atctatctaa agtcagcgat ctgaaaaatg tagcggatga attagtagct
     3601 ggtgtagata cttcttggat caatcgtaaa ggagacggtt atgacggctt ccaagaaact
     3661 tatggcttct ccttcaattc gatattacct atgcagattg gacttgttta tgacgcagtc
     3721 gaagcaggaa aaatggatat tgttcttggc tactctacgg atggacggat cgccagttat
     3781 gatctagtca tgttggaaga tgatcgtcaa ttcttcccac catatgatgc tgctccagtt
     3841 gtagacaaca agatattagc tgatacgcct catttaaaag aagccttaaa taagttaggt
     3901 cataccatca gtacggaaaa aatgcaggaa ttgaactacg aagcagataa taatctagtg
     3961 gaaccatcag tagtagcaca acgtttctta caagaaaacc actattttga agggaagtga
     4021 agcaattgga taaaatgaat gtctttcaac aatttttcta ttatttccag gaaaatggct
     4081 cctatatctt tgcacaattt atccgtcact tcctgatttc tatctatggt gtcctctttg
     4141 cagcagttgt cgggattccg gttgggatca tgatttctag gagacgtaaa ttagcaaact
     4201 gggtgattcg tttggcgaat atcatccaga cgatcccttc tctagccatg atctccattt
     4261 taatcattgg attaggcctg ggagtcaatg tcgtgattgt tactgtattt ttgtattccc
     4321 tcttaccgat catcaagaat acgtataccg ggatgatcca agtagacaaa aacatccttg
     4381 atgtcggcaa aggcatgggg atgactgcta gacaacgact attcatggta gagttaccac
     4441 tctctgtttc tgttatcatg gcaggcataa gaaacgcgtt agttgttgcc atcgggataa
     4501 ccgcgatcgg tgcattcgtc ggtgctggtg gtcttggaga tatcattatc cgaggaacca
     4561 atgcaacaga gggcacatcg attattttag caggtgcatt gccaactgca ttaatggcga
     4621 tcatcacgga ctggctttta ggtattttgg aaagacggct agatccagcc agccgtacat
     4681 cacgctgatg ttattgtaaa aaacaaaagc ttctgcgacc atttgttggt cgcaaaggct
     4741 tttttagttc tgtcattttt aaggcaataa tccactctga ttcttccctc cttatgggca
     4801 aagagttgct tttcttagct atttatccat tccgtatact ccaacttcat actgccaacg
     4861 ggttcatatc tgcatccacc gactaagttt ttttcttatc cgctaaggca ttttgccaat
     4921 ccattttccc atcactcgtc atggaattca taccacatga tctctaaata ctcgtctgct
     4981 tgttctttga attgggcatc aattcttaca tcctttggcg tggctttcat ttaagccttt
     5041 tccatcagtc ctcgattgtt cgatgccttc agctgcatca tcaaaactgg tgactgccgt
     5101 atcggtaact tcgatcatct tttatacaat cgggaacttg ccatcctcat ccttggctaa
     5161 aaacatatct agccattcca gcttctcgcc atgggaggtt tgcggttcct aaaaccggta
     5221 cacgattctt atggactgca tccgtcgcaa ctagacttgg cgatacgatg atccttcacc
     5281 agaagagcac ccccgtaaac caattgatcg atatattgcc aataagaaaa gacattggcg
     5341 taaaagatat cgatccctcg tgatgcattg ccgttgatac tgctattcat ggccgaaagc
     5401 gccacgactt taacttcttc cgtttgagtg acattcatgt tcggcaatgt cgctttggat
     5461 acccgctctg ctaaaggaac gacactggta ttgtatttcg tatctgtatc ttttccaaaa
     5521 gaccatgcca acgaatcctc tgggaaccaa taagacgact ccggttgatt gtccattgct
     5581 tttttcaaca tacttttggc tgtttctcga taaacgacat ttccaattgt cttcgcctca
     5641 ctttggcgat aatcgatggt cgttgccact gccaacacta acaacgatgc tccaatttta
     5701 ctgattttcc ttgtcacact tcttcctcct tgttttacat aaacccgtca acattccatt
     5761 cctaaactca aacaatgacc cttcaaaggt ggagatatag gaacaaatta aagaaatcat
     5821 gcaataaatc caagaaggtt ggttttagaa caaaaaaaga gtctcctttt tggggagact
     5881 ctttgttaac tatctaaaaa ggttggctct tctcacttca ctcataatgt gtccaggcag
     5941 aataaatttc tacaacttta ttttcctcat caactttgta aaccactcga tgctgtctat
     6001 tcagcctgcg tgagtatctg ccttcatgtg taggtctcag cttttcaaaa gactgcgtgg
     6061 gcatatatgg atcttccttc aatgtctgaa tcacttcttc aaattgtttc tttaaattcg
     6121 tttgcttgat ttttcttaaa tccactttcg cagagttttt gattgcgact gtatagttac
     6181 tcatcaaaga gcttcccaat caatatcatc gatattcgta aagctactgt cgtctttttc
     6241 gcggtctcgt actttatcta aagtacctgt ttgctcaaga aaaagggttt cttgaatcgc
     6301 tctccagtct tccagcccaa tcaatacagc attgtttcca ctacgatcac taataatctc
     6361 gatttcagta tgattttcat ttacctcttt caataactga taaaaatttt tacgagcagt
     6421 gctaggattc atcgttgtat tagccattgt ctctcctcct ttttctgata taattatagc
     6481 gtacttgatt aagtacgtca aaaaaataca ataaagcact taaattcagc aaacttttcc
     6541 tcttttttac atagagaaaa agaccccttt ttagggagga tctttgtcca cgctcacttc
     6601 ttactccttt acgactagcg gtagccaaac gtaaagtaat tggtaacaac gtatcatctt
     6661 tagcaaaggt cgaatccaaa gcctgtgagt ctatgcaaca acaccaatac acgaaggttc
     6721 ctcgttgcag tgtgacttga ttgatatctt gaataacctg aggatacgca cgaaagattt
     6781 gtaacggtta ttcaaaactg attttggccg tcaagtcttc tactgcctca aaaatgattg
     6841 cctctttcgt ctcctgtcat tcatgttcac tatcgatttg atacttgctt agaccgttgg
     6901 gtttgtgaat caccaattaa aaatggcact gagttatggt aacaaaaaat ggtcacttga
     6961 cgaggctgag attcctactt atgaagacac atggcactta agagtcgagg taaaggaaga
     7021 agctgaaaat ataggaggct gtacattatt tggcaaagga ttcggaaatt atgatcaaga
     7081 catcaaaatc aaaatgtttt acagctaaat aggaagaata ataacatttc atacgctgtt
     7141 cccatcttta atggcttata gagggcacct ttagctgaga ataagacaaa acagaccctc
     7201 tagcttcaat taagagctag agggtctgtt tttgatttta aaacttggtt tatttcttca
     7261 aatggtagct ataggtgctg acaacgatat tttcacgcca agacaatcgc aatcttaacc
     7321 gcaaacttgt ttggttatgg agaatattct gccagcgtct gtttggtaca aattgaggaa
     7381 tcaacacagt agttgtatag ttttgtttcg ctgcattttt acttacgata tctacataac
     7441 ggataattgg attttcaatc gagcgataag aagagtggac gattgaaaaa cggacatctg
     7501 ggaaatgctt tttaaattct gcttcgattt ctttttcttt ttcgacatct tcgtctagcg
     7561 aaacatgcat cgcaacgaca taatcaccga tcgaacgtgc gtaattcaac gcaccgatat
     7621 ttacccgtgt cacattcccg actaatacaa gaacggtatt cccgtcataa tcatgtagtt
     7681 ttgcatcatt ttctaaacga agctgttctg ctactttttt ataatgatca tggatcttat
     7741 aaaaaatgaa cataacgatt ggcataatga tgaagaaagg ccaaatgtcc cctaaccgat
     7801 agacaaacaa aatcgcaatg attgcatatg agatgaacgc acccgtaata ttggcaatcg
     7861 atttgcttaa ccatcttttt ccttcttttt tccatttgac gaccatccct gtttgagata
     7921 aagcaaatgg aatgaacacg ccgatagagt ataacggaat caagcgttct gttgagccat
     7981 ggaaaataaa cagcaatacg atcgaaccta gcgctagtgt gataatccca ttggaatagc
     8041 ctagtcgatc cccacgatct tgatacatgt gaggcatgaa tttatctttt gccagattgt
     8101 atgcaagaac tggaaaagca gaaaaaccgg tgttagcagc aactgctaaa atcagcgctg
     8161 tagcaaattg aaggacgtaa tacaaaatac catgtccaaa gacagcttta ccaatttgag
     8221 acaaaacagt gacttctttt tcaggaacga tcccatacca atagttgata aatgtgatcc
     8281 ccacgaagaa gaaacctaag atcaatgcca tcatcgtcaa tgtagcagcg gcattttttg
     8341 ctcttggctt cttaaagaat ggtacagcat tactaatcgc ctccacccca gttaaagagg
     8401 aagaaccaga agaaaatgct cgtaaaatca aagcgataga aacacccggc acagccgctc
     8461 ctggaagtgc agtagcttgc aatggcacag cacctgtcac gatattatat aaaccaacaa
     8521 cgatcagtaa cgagatcaca aggataaatg tgtacaccgg aaataatagg aacgatgcag
     8581 attccctcag cccacgaaga ttcagcatca tcagtagcaa aacgattgtc acagaaatag
     8641 cgacttgatg accgtagagt gctggcaccg ctgatgtgat cgcttcagca cctgctgaga
     8701 cagatactgc tactgtcaac atgtagtcga tcaataaaga gccaccggaa atcaagccag
     8761 cattctttcc aagattctca ctgctgacga catatgctcc cccgccatga ggataagcat
     8821 ggatgatttg acgatatgat agcgttaatg aaatcaataa aataataaca aaagcggcaa
     8881 tgggtagcga ataccagatg gctgcagcag acaaagcgac taaaacgaca acgatttgtt
     8941 ccgtaccata tgcaatagat gataacgcat cagacgacaa taatgccaaa gcagcaaatc
     9001 gggttaattt gtgctcatca ttttcagcag attttaaagg cttacctact aacaaccttt
     9061 ttaagtaatc caatttctaa actcctttac acacaatttt acttcaagtt ttatagtcga
     9121 aaatttatag taaacaagat gttatagtac gctcactgac cttaaaagtc aataaacttt
     9181 aagaaaataa ttggaaaaaa acagaaaaat atcaagcgct ttttcaaacg ctttaataca
     9241 gcaaaaatct aacatttttt tatgggcttt ttctttgaat tctataataa aaggatagaa
     9301 cttgacttag aaaatcaagt tctatccttt ttgatatctt aacttttgga agagagatgt
     9361 aaggctgctt tctacccatc attcttacta ctcgaagcat agcgcagttg tcacaacctc
     9421 ttgataaacg gtgtttaaaa gcttgcatct gcggtaataa gcccaaccaa attaaaaaca
     9481 taagaagctg tttttatttg gtcgttctta ttacttgatg cagagcagct tttttacaac
     9541 ctcttggtac acggtgttca atcagctgac cctcggtatt agctcaaaaa aacggcaaaa
     9601 tatgaggaac tatttttcca tttttttctc taatactcag gtcaaaacac tgatttcaca
     9661 acctcttttt ttaatgaaaa atatggctct catgcgccgc ttctttgaat ttcgtcattt
     9721 tttcttcgat gaacggatag attgccgttc ctacgattcc aaagacaaca aacaaaccga
     9781 tcatgatcca gattgctttc gtagcatttg gccaataaat tcctcctgca gattcccgta
     9841 acaagtttac cgcatgagta aatggcaaga acggattgat catttggaag aatttccctg
     9901 agacttggat cggatagttc ccgcctccgc cagagatcga cagtactaag atgataatag
     9961 cgatcccttt accgacatta tcgaataatg cagccaagac atagacgatc atcatgaacg
    10021 ctaaggcgat caagacagca aatgacacgc tgtagaccgg attttggaca tctactccta
    10081 gtaagaaaat attccctaat gtaacgatca agctttgcgc aatcgccatt gtcaaaaatg
    10141 tcagcataca agcaacgaat tgttctcgtt tcgtaaaatt gtttcggtct ttctttccta
    10201 aatagtattc tgtcgtagca acacttgaaa gaagaacagc gccgacccac agacatagtg
    10261 cagtataaaa cggggtgcta gctgaaccgt tatttgcgat tggataaagc gcattggttt
    10321 gtagctcaac aggttgtgta aagaagtcac tttcttttgt tgcgtctagt ttcaataatt
    10381 tgatgacttc gcctagatcg acttcttgtt cgccttttcg gatcgcgtct gctgcttttt
    10441 ggataccagc tttgatattt ggccagtcat tgttgatcaa gtccgcagca gcgttcacag
    10501 ctgtttcgac ttctggcatt ttttcattta ctgttgtcaa tgtaccggtg atttcatttt
    10561 ttagtgctgg ccaatcattt tggataaaat cagcggccaa tcctaatttt tgctgaatga
    10621 tcggtaattc attgttgtat aggtctgccc cttcattgat gccattgacg atggtatcca
    10681 tatttccatt cagcattgta ttggcatcat ggatttcttg tccaattgca ggcatttctt
    10741 tttgatattt ttctaaaatt gccactgcat tcgttacagt tgctttcgtt gagctgagaa
    10801 gcgaatcaaa gtcgatctgg tccgctttgc tcagtgcgcc ttgtgcagtc ccaatcgtat
    10861 cgatgacttg gtctagtaga gaagtgacag tggaactgat ttgacctgta ttgataccag
    10921 caatgatgcc attgatatta ttaacaacgg catcaatttg atctagcaat tcatcaatag
    10981 ccttcgtgtc ttcattttga acagctgtgt tcaaagcaga taggcggttt tgcagattgc
    11041 ttagcaaggg ttttaattga tttagctgat caataattgg ctgtaatttc gtatttccag
    11101 cacttgtttg caatttagtc aggaactcga ccagtttatc gattgcattt tgttgttgag
    11161 atattccgtc aataaagccg ttcaatacat tgctgatgtt tttcttttca tcatcagtca
    11221 gcttaccatc ttcaatagct gttcttacag tatctattaa cgaacctgtc gttgttgcca
    11281 cttgtccaat tgacttcaat gtaacatcta cactgtttgt gatgcttggt atcgcatctt
    11341 tcagctgaga ggcacctgat tttgtttggt ttgctagatc acttgcctga ttttctaaat
    11401 ttttcacatc tggcaagata tcttgtactt gttggatgat cgtcaagcct tgttttgctt
    11461 catcgattcc ttggttcatt gtctcttgga tcgaagcaaa atcgccgtcg atctctgcta
    11521 gttgcttacc ggcattttgg atctctggga ttttttcttg caaatcaagg atgatttttg
    11581 cttgatcttt caactcaggc attttgtcat tcaatgcaac gactttttca cccatctcat
    11641 ctactttagg gatgtaatcc acgaattcat ttgctttatt cagtttttcc ttgatttcag
    11701 gtagttgact ttgtaattcg aggacttgtt ttgtgtagcc gtcgatcgtg tccaaatttt
    11761 catctgttga taaaatcata tctttaactt tattgatact tactaggttc gtatcgatgt
    11821 cgtaacctat ctcattgaat actttcaata aagtcgaact ggctgttttg ataaattcat
    11881 ttgtgatttg cgattggatc gaacttgctc ctttatcggt gattttcggt gcaatcgcat
    11941 tgattttttc atttaccgta tattcgattt ttggtttctt gatatcacca ctagtaaaac
    12001 tcagcagatc ttctgagaaa tcctttggca agtaaatacc tgcgtaatac tttccggaac
    12061 ggactccgtc ttctaattgt tcttttgaat caacgaattg ccatcctaat tgtttatttt
    12121 tatgaagcga ttcgatgact tgctccccaa tggccacttc tttaccttga aactctgctg
    12181 gcttgtctgc actgtaaaca gcaattggca gctctccagt atttccatat ggatcccata
    12241 atgctttgat gttaaaccac gcatatagcg aagggatgat catgatcgcg atgatcagaa
    12301 gtgttgcaat cgggtttttg aaaatccgtt tccagtcaag caaatataat cgaagcgtat
    12361 ttttgattga ctgcatagtt ctcccccttc ttcttttttc agctgaatta ctttaccatg
    12421 tatttcatac ttttttcaat cggctttttt caaaaaagtc gaatttttgg acatatcacg
    12481 gcaattgtct cattgtaatc tcttacatat tgagaaataa ttaaggatta cagcctttac
    12541 tttatcatga aataaaaggg aaagataatt ttatgccaaa gtttttat
//
LOCUS       DANKKG010000077        12421 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000077 DANKKG010000000
VERSION     DANKKG010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.077"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..222
                     /locus_tag="PW004_001788"
     CDS             <1..222
                     /locus_tag="PW004_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDL2726760.1"
                     /translation="TEHRTIKYLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIR
                     GLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
     gene            226..843
                     /locus_tag="PW004_001789"
     CDS             226..843
                     /locus_tag="PW004_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDL2726761.1"
                     /translation="MDTVRDFILYLKLCNRTYYFKSEKDTSSLTLTLEEGVEPDKVTS
                     KVINQLKEKGSLKHLGEYEVLDTAMLTKGIGQILSTITYFITAVAGISLFIAGVGVMN
                     MMYISVSERTKEIGIRRALGATRKSIMLQFLLEGLILTLSGGLIGYLLGMALAYSVGS
                     LIKVNVSLDLFTILLAVGVSSVIGLVFSVMPASEAAKKDLIDILR"
     gene            1244..1849
                     /locus_tag="PW004_001790"
     CDS             1244..1849
                     /locus_tag="PW004_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HDL2726762.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            1865..2437
                     /locus_tag="PW004_001791"
     CDS             1865..2437
                     /locus_tag="PW004_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2726763.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2689..2946)
                     /locus_tag="PW004_001792"
     CDS             complement(2689..2946)
                     /locus_tag="PW004_001792"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726764.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(3072..3887)
                     /locus_tag="PW004_001793"
     CDS             complement(3072..3887)
                     /locus_tag="PW004_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726765.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            4404..5729
                     /locus_tag="PW004_001794"
     CDS             4404..5729
                     /locus_tag="PW004_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HDL2726766.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            5722..6072
                     /locus_tag="PW004_001795"
     CDS             5722..6072
                     /locus_tag="PW004_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726767.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            6069..6239
                     /locus_tag="PW004_001796"
     CDS             6069..6239
                     /locus_tag="PW004_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726768.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHKYEYYIGY"
     gene            complement(7143..7418)
                     /locus_tag="PW004_001797"
     CDS             complement(7143..7418)
                     /locus_tag="PW004_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HDL2726769.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(7390..8343)
                     /locus_tag="PW004_001798"
     CDS             complement(7390..8343)
                     /locus_tag="PW004_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDL2726770.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            8955..10448
                     /locus_tag="PW004_001799"
     CDS             8955..10448
                     /locus_tag="PW004_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HDL2726771.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            10583..10879
                     /locus_tag="PW004_001800"
     CDS             10583..10879
                     /locus_tag="PW004_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HDL2726772.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            10980..11831
                     /locus_tag="PW004_001801"
     CDS             10980..11831
                     /locus_tag="PW004_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726773.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            11852..12135
                     /locus_tag="PW004_001802"
                     /pseudo
     CDS             11852..12135
                     /locus_tag="PW004_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         4268 a         1919 c         2317 g         3917 t
ORIGIN      
        1 acagaacatc gtaccattaa atacctgaat aatttgattg aacaagacca tcgtccagta
       61 aagagacgca ataaattcta tcgaagttta cgcactgcct ctaccacgat taaaggcatg
      121 gaagccattc gaggattata taagaaaacc cgaaaagaag gcactctctt cgggttttcg
      181 gtctgtactg aaatcaaggt attattggga atcccagctt aaatcataga taccgtaagg
      241 gattttattc tttatttaaa actttgcaac agaacctatt attttaagtc tgaaaaggat
      301 acatcgtctc taacgttaac tttagaggaa ggagttgaac ctgacaaggt gacctctaaa
      361 gtgataaacc aattgaaaga aaaaggtagt ttgaaacatt tgggggaata tgaagtgtta
      421 gatacggcta tgttaacgaa aggaattggt caaatcctca gtacgatcac ttattttata
      481 acagctgtag caggtatatc tttatttatc gcaggggtag gtgtaatgaa catgatgtat
      541 atatctgtat ctgaacgaac aaaagagatc ggtataagaa gagccctagg agctactcga
      601 aaatcgatta tgctgcagtt ccttttagaa ggtttgattt taactctttc aggagggtta
      661 attggctatt tattaggaat ggctcttgct tatagtgttg gttctttgat aaaggtaaac
      721 gtgtcacttg atctatttac tattttacta gcggttgggg tatcttcggt tattggactg
      781 gtcttttctg taatgcccgc ttcagaggct gcaaaaaaag atttaattga tattcttaga
      841 taaaatcaga atgacaagaa tatttacgtt ctagattcta aaataaaaca caaagagacc
      901 aaccaggcac cctattgttt ctttgtagta tagagatatt ctttggcttg taagagatca
      961 atagagttag gtgaccggag ttcgaaataa aggaaatttt tgattgtata ggagaattct
     1021 ttatccctaa tatttagaat tttgagtatg ccatgttgat gttttagatt ctctataaat
     1081 atagaggtgt atcaaaggtc aactagactg tgcaaaatag agacacttga aaaatctatc
     1141 gggggtcaga aacttaatag tttttgacac ataaacaaaa tcgcgatata atggggttga
     1201 gagtgtttca taaacttatg tcataaagtt aaggagattt cgcatgattt tagagaataa
     1261 attgggatta accaatcaag ttgagttggc taaggttgaa gaaaaactca gtaaacaaaa
     1321 agcgaaagaa ttgtacgatt gtggaaaaat aaatgaaatc gaagttggta catttaaagg
     1381 tttatctgaa attcatgaat ttctattttc ggatatttac gattttgccg gaaaaatccg
     1441 atcggtcaat attgcaaaag gaaattttcg atttgcacca gttatgtatt tagaacattc
     1501 gttacagcat attgatcaga tgccacaaac ttcttttgaa gagattatca agaaatatgt
     1561 ggagatgaat atcgctcacc catttcgaga aggaaatggc cgaagtacta gaatttggct
     1621 ggatctcatt ttaaaggaag aattgcaaaa agttgtagat tggaacttaa tcgataaagc
     1681 agactatctt tctgcaatgg agcgaagtcc cataaacgat ttggaaatac gctatctaat
     1741 ttccaatgct ctgactgata aaattgacga tcgcgagtta tatatgaaag gaatcgatgt
     1801 cagctatttc tatgaagggt attctgagta tacgatagat gatttgtaaa agtaggtgga
     1861 ccaaatgacg aaatatggat atgcacgtgt ctcaaccgct tctcaaagta caaaagaaca
     1921 aattcaacag ttgcttgaga acggcgtaga gaagaagaat attttttcag aaaaatttac
     1981 gggtacaaaa cgggatcggc caaaattcaa tcagctgttg gctggtctga agcctggaga
     2041 tgaaatagtt gttacgaagc ttgatcgatt tgctcgtaac acgagagaag cattagagat
     2101 cattgaacct ttactcgatg ataacgtaac gatcaaggtt ttgaatctag gaacgattga
     2161 aaatacgtcc atggggcgaa tggtgacacg gacgttgttg agtgtagcag aaatggaacg
     2221 tgatatgatt gtggaacgta cccaagaagg aaaaatgttt gcaagaaaga ataatcctaa
     2281 tttcagagaa ggacgtccga aagcaacaat cacaccgaag aagaggcacg catatgaact
     2341 gcttacttca ggtaaatcgt ataaggaagt tgaagctata accggtttca gtcgcagtac
     2401 gctttttaga ataaagaaga aaattgaaga aagttaaaaa agaagtctca gcgaaccaga
     2461 caggtaaata cttaatgcgt tctagatatg agtctattag catgttgtga aatataatat
     2521 ttttttattt acttgatttc tgctcaaaaa atgtgtatgg taactgtaaa aggtaaagta
     2581 attaaaggta tgctagccac gcttactata ttaggatacc atcatatatt tataatgaac
     2641 gatttgttcg tctaccaaac aacacatatc gttcagcgta agtcacgttt aggaacgtgg
     2701 cttttttagt gttattatcg ataaataatc tattgactct attgatttta tctccatatc
     2761 cctacggtgt tgattgtctt tagatctttc atttaagaac caaataacta tcataaaaat
     2821 cagcactgct gctaatataa taaacagcat tcctattgtg atattaccta ttgattttac
     2881 cataactacg attaaagtaa taatggcatt caagtctcct ttgttctcta tatataaatt
     2941 agccataaat catcttcctt tcaaattaag atacttgttt gataccaata aatagtaagc
     3001 gtggctagca tacctttttc tgtaatagca agtgttctaa tagtccaaac ttaatgatgg
     3061 accgatgcta gttatagtac agtaatgttt tctttcttat ctagattttt agtaagctct
     3121 atcagcaaat ctaacaaatc tgagtccttt tcagaaacta aattaaatct agtgtttaca
     3181 acaaagtttt catgttctat attgtttaga ttatttgcgg aaaggagggc ttctagatct
     3241 tgacattcaa atctatatgc atcgtcataa atcttactag aaggagtgct taattcgaaa
     3301 ttacttattt tctcatcttg gaccaagata aatttataga tagtatcttt ttgtgttttt
     3361 cttttaaaaa cacaacagag tgtgttataa ggaacgttat aattttcatc agcctcattt
     3421 tttctccatt caaacaaatg ataaactgca accaagttgc catcaatttc atggacagtg
     3481 ctctcggtaa catcctttaa agaactatca agggctaatt tatagtaact agagagagga
     3541 agtaaaattg tcgatttact gagatggtta tcgtctatac gtgcaatcat acctaaatca
     3601 actaaggttt tattccaggt gtttatagtt ttagttgtca ctcctagaga ctctgctatg
     3661 gtattgaccg aatgccagga atccccgcct tcattctttg ccttaaaaca gtaatacaga
     3721 tataaattaa tagctttgtt attcggtaaa tatttttcaa gttctgttgg tagaataaag
     3781 taagaatcct taatgttttt tctgaactgt ctccagtctc tgtagttttg tttataaagt
     3841 tgtgaaacgg tttttccacc tttagaaagt cctgctatct taaccaatct tattgctcct
     3901 ttcttgaatc tgtcatgatc atacagtatt ttttacttgt agtccagcac cttaatatat
     3961 tatcctatgt aattaatttc gtactagata gtaattattt tttatttaat tctctagtca
     4021 cttacaaata agttatataa actacgatta ataaattatt acaaaaacta ttaagaaaac
     4081 ttctacatac aactattaca aaactattaa gaaactgaat actcctattt tttgttttta
     4141 aggaaagttt tttctttttt gaatttctta tttttttcta atataactaa gcaaaactgg
     4201 ataatacgac acttattttc aaattttcac aagactaata gtaaatatcc atttaataaa
     4261 gtgatacaat gtgttgattt tacaaatcaa cagaagaagt agggaaataa cttacctatg
     4321 cttctttttt tgtacattta atcgattctt ctttacaaaa acgaacgtat gtttgtataa
     4381 tgtggaagaa tggagtgtta tttatgatgg attattcaaa agagccagta aatgattact
     4441 ttttgatcga tatgaaaagc ttttacgcaa gtgtcgaatg tatagaaagg aatttagatc
     4501 cattaacgac tgaattagtc gtcatgagtc gagcggataa tacaggatca gggttgattt
     4561 tagcttcttc accagctgca aagaagaagt acggtatttc taatgtcagt cgaccacgag
     4621 atctaccgca accttttcct aaaacgttac atgtcgttcc accacgcatg aatctctata
     4681 ttaagcgaaa tatgcaggta aacaatattt tcagaaggta tgtagcagac gaagatttat
     4741 tgatttactc gatcgatgaa tcgatcttaa aagtgaccag atcaatgaat ctttttacga
     4801 ctgaaggaac aagaagtcaa cggagaaaga agctggctca aatgatccag gagaatatta
     4861 aagaagagct aggattgctt gctacagtag gtgtcggaga taatccctta ttagctaaac
     4921 ttgctttaga caatgaagca aaacataatg atggattcct tgctgaatgg acgtatcaaa
     4981 atgttcctga taaagtatgg aatattcctg aaatgacaga cctttggggt attggatctc
     5041 gaatgaaaaa aagactcaac caaattggta tcatgagtat taaagactta gctaattgga
     5101 atccatacac tattaagagc catttaggtg tgattggctt acagctgtat tttcatgcga
     5161 acgggatcga tcgaacagat atagcgatcc caccagaacc gacaaaggaa aaatcatatg
     5221 gtaattcaca agtgttacca cgagattata ccaggagaaa tgaaatcgaa ctggtggtaa
     5281 aagaaatggc ggaacaagta gctattcgta tcaggcaaca taactgtaaa acaggttgtg
     5341 tccatttata cattggcacg tcgatcttag aaacgagacc aggtttttca caccagatga
     5401 agatccctat aacagataat acaaaggaat tgcagaatta ctgtcttttc ttgtttgata
     5461 agtattatga aggacaagaa gtaaggcacg ttggaataac ctatagcaag cttgtgtaca
     5521 ctgattcgct tcagctggat ttatttagtg atccacaaaa gcaaattgac gaagaaaatt
     5581 tagataaaat tattgacaag attcggcaaa aatatggttt tacgtcgatt gtacatgctt
     5641 cttctatgtt agaaggcgct cgtagtatta ctagaagtac attggttggt ggccatgcag
     5701 gcggcgcagg agggcttaaa aatgattgat tataatgatc gtggacgttt aaagtgggca
     5761 ggtttttatt tatctgatca tactgaaaaa attgatgccg acaatgaaca acgagccaat
     5821 caaaatttgg caaaagaaca aatgacgact gaagaggttg cggaagtatt gaacgctgca
     5881 gtcataaaaa atagatcagt atgtattcaa aaagaagaac gaaacgctga aggatactat
     5941 ccagaagata ttgttagcaa gatcttaggt agtgatgagc tggggatcta cgttggaaaa
     6001 aacaaagttc actacgacga aatacgtcat atcgaatttt accaggataa taaatggggt
     6061 cgattagaat gaacattatc cagcaatatg agctaaagta tataaccttt gatcagctat
     6121 cagaagagat ttggggttat ggccagcgtt tgataaatga ggttggtgtt gaacgcttct
     6181 ctttctatgt agaagctgca gcaggatacc ataaatacga atactatatt ggctattaaa
     6241 gtgcaataaa attatatttc ttttatgttt tacattttgt atcaattctg ataaaattac
     6301 ctataggtaa ttaaaaaata taaaaaaact agttacaacc ttaggacgtt gtaactagct
     6361 tgaagcatac tagagctaac taaaatagca ctagaaagca atgttaaacc cgtccaacaa
     6421 acttgattta acattgagac cagcttattc agctaaatat agttgaataa gcttcttttg
     6481 ttttaaaaaa acaaaaggtg tactacgaat aacgcaagta aaagaaatac tattacaacc
     6541 agtaggctac aagtaagtat tactaaacat tgcgtagtta gaacctttgc tctattgtct
     6601 aaagccacaa aattagagat cagctttatg atgctaagag cgagctgcat ccatgcggac
     6661 ataggttctt ttgacactat taattctcct ttttaggata agggaaattg ctagtagttt
     6721 tttcattagc accatccccc ttcatttgat tgagtctagt gctgctgtga tagtttgctg
     6781 tttaatatgc ttacattctt attataacat aaatgaaaca aacacatggt tagaggtcgt
     6841 atcaatcaaa atagtgtctc acaaacgatt tattacttac tcctgctgac taacggactg
     6901 ttcttcagta actgtaacta tatgtttgtt aaaaccacca ttacctgtag ataaaaacca
     6961 atatatttac tatactcact atattaaata tatttagtat agtaaaaact agaaacaata
     7021 aaatttgtga atgttgtata cccccaaaca atacacatat tttgaaggat taggattaat
     7081 aaagctaaaa atatagtaag tatactaaat atatttacta tatctagtat acttactata
     7141 ttttaatctt ttgatcgtct attatatata atccgttcct cgtcgtttaa agtatcaaca
     7201 taggcgtcca ttgcaagttt gactatttca tacataggaa tatcttttgc ataactaaga
     7261 tcccgaatgg tttttagtac gggaggatct acttgaactg gttttctttc tttcgcagtg
     7321 tactttcgtt cccctaactc gacattaata tcatctttaa ttgaaatatc ttcagcacca
     7381 attttcttct tatctttgtt cgttggtaat aatgccattt agtttaatct cctttccaag
     7441 aggtaggata tgattggcca ttgagtcagc atatttaggt gtataatcaa atccttcaat
     7501 atcaccatgt tgttcatagt attcaattct ttcttctaat tctgtaaaga tgtctgcaaa
     7561 aatagcccac catcttcgat ctacataatc atttaattgg ataccattct caccatacat
     7621 ttctaaccga tccgagcctt taacaatgct tttaaaaatg tttgattcac catacttatc
     7681 aattgttttt tgatagtttg cgtgttgaga ggaacgtcgc actgagaaaa gaacaggcaa
     7741 aattccaatg atttgaagat gagactcttc aaaattcgtg acaatcggaa ctaaaacttt
     7801 attaataaaa tcttctgttc cttccattgc gtaccgtttt aattcttgca ttgcaataat
     7861 ataatctgca gcagttaaga atgcacgaac cacattatct gttgatggtg ggcagtcgat
     7921 aaagatataa tcgaaatttt ttctcaattc ttctaaaggt ttaatgaagg ccaatgatct
     7981 cttacgacga tcaggatatt tttcggacaa gtattcatta agttcttcag atccaggaga
     8041 gccagccata atatataaat gtggacttac ttgagtaata cctgaaaccc agtccccttt
     8101 tttgattgct gcagacatgg atcgatcaaa gctagtaatt tgatacgttg atccaatgtt
     8161 ggaagtagta tttctttgtt ggtcataatc aattagcaac acgtcttttt caaaaagttg
     8221 actagaaaca atcgctaata aatcattgtc tgtagtctta ccaatgccgc cttttctacc
     8281 tgtaagcatt acaacttttc ctagtttatc tgatgtttta aatgcagtcc gtaagttttc
     8341 catagttccc tccaatttcc tatactaaat atatttagta tacttcatat acttaatata
     8401 ttcgatatat ctaatatact ccaaattgat aagaattcag actttataag gaatatagtg
     8461 gatatactaa atatatttgc tatattcagt atatcagata tattcaaaat gtcaaaatat
     8521 atttgtgcgt tttgcgttga caatttagag tgaaaagagg taaacttaat tctatagaaa
     8581 ccacagataa ttcactattt acaaagtagc aaaagcagtg gtactcttta aacagaaaaa
     8641 ttacataaaa aaaagagacg gaaataattg attttagcag aatcaattat ttctgaatca
     8701 catggttagg acatgtgttc acgctcgtct caagaatatg taagtagcta ccaactactt
     8761 atattaaaag ttgatcggat accttttccg attgccttta ttctaccaaa aaaaggcgag
     8821 caactcaaca gaagaacgtt tatttagtgt acttaaatta aatattctta aacactgaag
     8881 agagaacaca tactatggct gagattaaag ctaaactgtg ttctcttttt gtttgagaaa
     8941 ggacgttaca atcgatgttg agcgaaattt attcagctat tcttaaaaac ggcttacgta
     9001 catacaaata tcgaaacagc accatgaaac ctgcaggtca tattgattcc agtaggagtg
     9061 gtgctatctt tgcgtaccgt agcaagcaat taatgaatgt tgggcgtggt atggtcatta
     9121 cttcggaaga agctatttta gagaacgaaa agaaactaac gcattggaca cctaatacct
     9181 atcgctttgg tacttatgcg gatgattatc gaacagtggt taaaggtcat tctgagaaaa
     9241 acttaagtca aatcaacaca tttgtagttg atatcgattc aaaagagaac catcaaggcg
     9301 aaattatttt agcctgttta gatcaagtgg gctacatgcc aacgctaatt ttagaatcag
     9361 atcatggtta tcaagtttat tttgtactaa agacaccagg ctatgtcaca aagaaaagca
     9421 atttcaaggt gatcgatgta gcaaaaaaaa tttcaaaaac aattcgtcta cagttggctg
     9481 aaaaattgtc cggagtggat ctaggatgta accacttcgg aattgctcga ttcccacaag
     9541 aaaataatat tgtttttaaa gaaatgaact atcagtattc ttttgctgac tggattcaat
     9601 ggtcgatgaa gcttgaatca gatcaaaaaa caaaagaaga tcgagaacga aaactaattg
     9661 tctttcctga gaaaaaagaa attcgtcaag tagatgaacc gtggttcgat atgttacttc
     9721 ataaagcaaa catcgtcggc ggagaaggac gtcttggtcg aaataatgtg attttcacgc
     9781 tttctttagc ttattactct tcaggttatg cattggatac ttgcgaatac aatatgttca
     9841 cattcaacga acggttacag gaaccgctat ctgaagcaga attagtaaag attgtacgta
     9901 gtgcttattc tggtaattat caagcagcaa gcagagaatt cattattgaa ttgtgccagc
     9961 aatgggtttc accaacctta aaaaataatg atctgttcat tcaacgacgt ggctggtgga
    10021 aatttaaaaa gccacgagat caaagaaaat atagtcataa aaatgaatgg aaagaagatc
    10081 tattggctta tctaagcgaa aaaagtcttg ctagtgagcc ttatttgaac ctgtcaaaaa
    10141 aagaattggc ttcacaacta acgatgccca agcgaacact tgataagatc ttaaatgagt
    10201 tacgagagga gaataaagtc ttctacaagg taacacgagg aagaaatggc ggaatgatca
    10261 ttgcttcagt caaaacgttg ttcgcgaggg ttattcaact aagtaaagaa aagagactag
    10321 attattcaac aaggatcaca gaggtatttg gactcaacga gagcttagta gaagagacat
    10381 ttagacaatt atcaaccccc ttgaaccctc ttaaagaagt gaatcttttt gaagtagata
    10441 caggttgaaa aataaaaggt gcaaattgcc ttatacaata tatctatgat acttctgtag
    10501 gtgttgctcc ctttgcctag ctacgtgtag gtggtatact tataactgcg tatgcagtct
    10561 tatgtatcat ggaggaagtt gtatgtcgtt aaaaaattat gtctattcac atcctgttaa
    10621 tgtgtttatc attggtcgtt tgggattatc tgtggaagtg ttctgtgagc tttatggttt
    10681 taagcagggg actttgtctt cttgggttac cagggaaaaa acagttgctt ctttgccgat
    10741 tgagtttctt catgggttgt ccttagcttc tggggagact atggatcagg tatatgattg
    10801 tttgtgtgtg ttagagcagg agtatattga gtataaaata gctaacgagt taagaaaaag
    10861 gaagaagtat atccagtagt agtttttatt tacttaaaaa attggttcat tgagcaacct
    10921 atcgctgcta aagtagcaaa ggaaaacgtt aaaggaaaga atttagggaa gaaatcgaaa
    10981 tgaaatcgga cgactggatt gaacacacct ggcttcctct gaaaagaaat agcaaacgaa
    11041 agaaattttg ggtattgcaa ttatggccat taggaaaact ttgtagttac ggggcacgac
    11101 ttattcttga tttaaccgtt tactcgattg gagtatttgg aacggcttat tacttgataa
    11161 atgatcccaa taggattgca gcatcagaca tttttacagt gctaatgacg ttgatttttg
    11221 tcatgctatt gtctattttt actcaattag tcatgcaatt taccaggcaa aaattaacag
    11281 caggaaaact tttcaaaagc ctattcgatt taattgttgt aaatgcaatt ctagcgactt
    11341 taataaaaca aattgaacaa tctggaagaa caacaaaagg agtgaaggat tttttattga
    11401 cgtttattca ctttgtaaat ggacacatgt atttgtttga agtgggtatt attctctttt
    11461 ttgtcgggta tgtatccacg aaacttatta aacgttcaat tgaaaaagaa attgaaattg
    11521 acctgattcc gacacttctt ggttatgagt acaatgttga ttttggaaga tatgacgaat
    11581 tgatcaatac ggatatcgtt cacacgagta tccatgaatc tactgttaag ggcatcagat
    11641 ctattttaaa aaatggccag gaaatgaaag caatagatcg aagaaaagag atctacaatt
    11701 tttatgaagt cgaagcaagc ctacgatatg gcatcggaaa attgtggtta gatggaagta
    11761 atattcgtca aaacaaacca tacatttttt ggaacagtag tgatttagaa cttccaatga
    11821 aagataaata attatggaga atcgtttgaa aatgattcct ctatacagga ttgtgcttat
    11881 agttggcatg gagttaaaaa ccttaaagaa ggagcagtta ttcaaagtga aagaacgtca
    11941 taatcagtta ctatgtagta aaacgggtac gatgaacatt gaataccgaa cagaatattt
    12001 agaattaacg aatcaaactg attttttgtt gattcgaaag caacaagagc aagaaaaaaa
    12061 ttaatggcta cttatagcca attgattgat gaatgggaac aattcaagga atctaaaatt
    12121 ccagaacgtc tctaaaagcc tttatttgta aagagcataa ggaaacgtgg gaacactttt
    12181 taaaaaaagt gttccaatag gctgtgccta ttacgtttct caaaaataaa tgtgggaaca
    12241 aaatatttaa aaataggata gtgaaaggat agtgaaggat agtaaaagga gggtagtagg
    12301 atagcaaaaa ggataggctc cttttcgcta tcctttttaa attggaggaa ttaatatgga
    12361 agtaagaata agaaatgtgg atgagaaaat cgtaagagaa attgatcgaa gatgtgacga
    12421 a
//
LOCUS       DANKKG010000078        12339 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000078 DANKKG010000000
VERSION     DANKKG010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.078"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(<1..582)
                     /locus_tag="PW004_001803"
     CDS             complement(<1..582)
                     /locus_tag="PW004_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDL2726774.1"
                     /translation="MKKYFIIEDNQFHSEWIIKELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLL"
     gene            643..1896
                     /locus_tag="PW004_001804"
     CDS             643..1896
                     /locus_tag="PW004_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDL2726775.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGFIALMVVTLL
                     FPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGLRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            2197..2445
                     /locus_tag="PW004_001805"
                     /pseudo
     CDS             2197..2445
                     /locus_tag="PW004_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            2567..2893
                     /locus_tag="PW004_001806"
     CDS             2567..2893
                     /locus_tag="PW004_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HDL2726776.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLQLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEF"
     gene            2961..3674
                     /locus_tag="PW004_001807"
     CDS             2961..3674
                     /locus_tag="PW004_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HDL2726777.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYLVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PIFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEEKEIVTLITCGDMDAVTRLVVQGELESVTP
                     IKDATDDMRSAFNLETKTF"
     gene            3984..4836
                     /locus_tag="PW004_001808"
                     /pseudo
     CDS             3984..4836
                     /locus_tag="PW004_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            4957..6822
                     /locus_tag="PW004_001809"
     CDS             4957..6822
                     /locus_tag="PW004_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2726778.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            6870..8279
                     /locus_tag="PW004_001810"
     CDS             6870..8279
                     /locus_tag="PW004_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HDL2726779.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            8387..9088
                     /locus_tag="PW004_001811"
     CDS             8387..9088
                     /locus_tag="PW004_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2726780.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            9247..9726
                     /locus_tag="PW004_001812"
                     /pseudo
     CDS             9247..9726
                     /locus_tag="PW004_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            10574..11149
                     /locus_tag="PW004_001813"
     CDS             10574..11149
                     /locus_tag="PW004_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HDL2726781.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            11149..11589
                     /locus_tag="PW004_001814"
     CDS             11149..11589
                     /locus_tag="PW004_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HDL2726782.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            11605..12306
                     /locus_tag="PW004_001815"
     CDS             11605..12306
                     /locus_tag="PW004_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDL2726783.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
BASE COUNT         4120 a         2005 c         2612 g         3602 t
ORIGIN      
        1 tagtaaaaag taagcatact tttccaactc tgtcttcaat tgttttaaag aagtattcac
       61 taatttatct tctgtagttg ttttgaccat aacttggttt ttcaaacctt tgatactttc
      121 aatatataaa atttcccgag gatacaggac gagacttcca cctttttgat caataaagat
      181 tttatcgaca tttacgattt gctgttcttt ccaattgatc tgttctagca cttgacgaat
      241 ttgttcagat aaattcctgt cttttctgat atatccaatc ggaaggattt ttcgattgat
      301 gatctctact gcaaaattcg tataagcggt gaaaaaaaca atattactat gtgcattttt
      361 tttacgtatt tgttcggcga catcaatacc tgtgagtgat tgcttcaagt cgatatcgat
      421 aaagaaaaca tcgtgattat caatagtacc caactggatc aactgattga tttgagagat
      481 agaagtgagc tgcacgatat tctcgatatg atgattgacc aattctttga tgatccattc
      541 gctgtgaaac tggttatctt ctatgataaa atactttttc atatgcaggc tcctattaaa
      601 aaaatatgtg tacattttga aacaaaacaa tggaggaata agatggtcgc aacagtgaca
      661 ctttatttga cctttacttt gattttagat ttaggtatct atttattctt ttcgaaacaa
      721 ctgctagtaa aaaacagctt tggttttata gctttgatgg tggtgacact tttatttcct
      781 atccctaaag tgagctttcc ttacctagtt ggcttattat tgatgtttct tttttatttt
      841 actacgaaaa acctgtttat cagctgtttg atcagtaatc ttttttttgc tatcactgga
      901 ctcagctttt ttctggtttt tgatctgatg gattatttag gaaagagaca tcttggaaca
      961 gcaagttttt ttgcactact tatcgcattt attttctatc tattagtaaa aagtatagac
     1021 aaacagacga gatttctcaa cttgcttttt acttatccaa ctgaacacta ttttggaggt
     1081 atttgtattt tattaagctg gggcgtgatt tacttggtat tgggaagtgt tcacttccgc
     1141 tcatctaatt atctgcttct atctcttttg tcgatagtca tcagtttttt ttctttgttt
     1201 ttttccatta tgttgatcag tttccaagta aaagaaagac aacatcagga atcgcttcaa
     1261 ctgtatcaaa taaatgaaga atattaccat catctagagg aattcaaaca tgattataaa
     1321 agtctactgt tttcattaaa aattactctt caggaaaata aaaaagaaac gttggaattt
     1381 ttagaaatct tagaagatca ttctgcagct atttttgaaa aaccgcaaat acaacagcta
     1441 acaaatattg tatccccagc tgtcaggggt gtctttttaa aatttctaga aaaagcagaa
     1501 tcggagaaaa tcccgatcca agtagttatt cctgtcgaag tgaaaacaat cccgattgat
     1561 ctggttgttt ttattcgctg tttatctatt tttatttcta acgcattcga tcatcgtgta
     1621 gccgatcaga gtccgattac gatcaatctg ctggaagaaa aagagggatt acgttttagt
     1681 atagcgaaca aggcaaatcc gacgaatttg acgctatcgg agatggtcaa aagaggaaaa
     1741 acctctaaaa aaaatggcgg gttaggtcta tacagtgcga aaaaaatgtt ggatgtttac
     1801 gaaaatgcag aactaaaaat cgaattttca aaacagaatc actatttttt tgtagaaatg
     1861 tatctggcat ctagaaagag caaatcagcc tactgactta ctgtcaaagg ataagcttga
     1921 ggaaagtcta cacggagata gagacaacaa cacagcatat tcaaaagaaa cagaagcgtg
     1981 atgaccgttg tcacccttct gtttcttttg ctatgtagtt aaacaaagta gttgacactt
     2041 ccgctactta gtattagtac gaaagtatac ttcatgatac taagtagata agaagtgaaa
     2101 agcggaaccc acgatcgtca aaatgctaac tgctttgctt acaccagata aagggaagag
     2161 caatatcgct tattttaggc gtggagaagc aagaaaatcc ctacgatcac acaaaaaatg
     2221 ctgacccagc agttaagaga gttagaacaa gatggcattg tccatcgaaa agtctataac
     2281 caagtcccgc ctaaagtaga gtactcgctc accgaagaag gaaaatcatt aagagagatt
     2341 ctagtagcga tgtctgtttg gggagaagaa cgtatcgaaa aacaaaaagc ctcaggggaa
     2401 gaagtcacat tactgcatac acatggtggc tacctaaatt actgatttct atgacattta
     2461 ttttaaataa aagaaaacag gagttttttt aaaagcatca tctatttttt atgtaaaaaa
     2521 atggtatgat taatcaagta agaaatgatt ggaagaaggc aagcaaatga atagcatgga
     2581 aaaagttaac cagtttagag atgaacgtaa ttggcgacaa tttcataacg aaaaagattt
     2641 ggctatttct atttctttgg aagcatctga attgttagaa ctttttcaat ggaaaacgcc
     2701 ggaagaagta aaagaaacac aattagaacg aatcaaagaa gaactggcgg atgtattgat
     2761 ctattcatac atgatggcag acaaccttca gctggatcta gatgagatca ttgaagaaaa
     2821 attgatcaaa aataatttga aatatcctgt agaaaaaagc gcaggtgtac gaaaaaaata
     2881 cacagagttt tagaaatgtg tcattcggct gttggaaaga gcaaaagaaa aacagtaaaa
     2941 tgagtctgga gggagagaaa atgaaaaaaa aaggaaaagc aaaaagatgg ctagtcaata
     3001 tcctcttgtt cctcttgtta ttagtaggac tagctttgat tttcaatgaa cagatcaagg
     3061 attatcttgt cagagagaca ggagataaat atgctatagc gaatgtaaca aaagaagatc
     3121 tgaaaaagaa taacgataaa gatgtcagtt ttgactttga tgcggtagaa ccaatgacga
     3181 cagaaggggt catgaggtcg cagatgagag gaacagatct accagtgatt gctagtatcg
     3241 cagtcccttc agtttcaatc aatttaccga ttttcaaagg cttggataat acgtcactac
     3301 tttacggagc aggaacatta tctccagatc aagaaatggg aaaaggaaat tatgccttag
     3361 caagccatcg tgcaacgaac cctgaattgt tgtttacacc gctggaaaat ttagagatgg
     3421 gtgccaagat ctatttgaca gatttagaaa atgtgtatac atacaaaaca ttttttaaag
     3481 aaaaagttgc tcctacagat acccaattac tgaatgaagt cgaggaaaaa gaaattgtca
     3541 cactaattac ttgtggcgac atggacgccg tcacgcgact agttgtacaa ggagaactgg
     3601 aaagtgttac accgataaaa gatgcgacag atgatatgag atctgctttc aatctagaga
     3661 caaaaacatt ttaggatact gggacataag tcgttacaga ttaaaaacag ccaaaggata
     3721 tgaatcgata aggaaaaacc aatttgctga aaaaaagagg aacgcaaaga ggtgcatctc
     3781 tgatttggtt gttttctaga tagacttgtg ttccagtttt tttatttttt atatgaaaag
     3841 gcttgcataa ataaaaattc gatggtatga tgattttgaa taagaaatgg attgttactg
     3901 tacggcaggc aaaaccagaa ttcagctaag aaacagtagg cgaattctag gtttttttat
     3961 ttttcttgag agggcgaagc aaaatgaaaa ttgataaaat tttgaataac aatgtcgtca
     4021 tttcgaaaaa tggattcggg gaagaagtcg tttgtatggg aaaaggacta gcttttcaaa
     4081 aaaaagattg gggacgagat ttcgccagaa gcggtccaaa aagaatttat tttaagagat
     4141 tcttttgcta aaaaccaatt ccagcagctg atggctgata ttccggcaga ggagatcgaa
     4201 tgggtcaaac aagtagtcga actggctgaa gataaactaa aagttgaatt ttcgcctaat
     4261 atttatctga cattgaccga tcatcttcat tatgcgatca cacgagcaaa agaaaatatc
     4321 caactgccta atccattatt atttgaaaca aaaaaatttt atccgaaaga atatcaagca
     4381 gcgaaagagg ctcttttgtt cgtgaaacaa aagacgggtg tggaactttc tgaggatgaa
     4441 gccggtttta ttgcctttca ttttgtgaat agccaacaag gaaacgaaga catgcaagtg
     4501 acgatgaccg ctaccacaat ggtaagagat atactgagta tcatcagtaa attcttcggc
     4561 atcgtgttcg atgaagaatc tttgaattat cagcgtatca ttactcatct gcagtttttt
     4621 gcccagcgtt atctcaaagg ggaaggatct gacgagcaag atgaatttct ctacgcatta
     4681 gttcaaggaa agtatccaaa agcgtttcat tgtgtcgaac ggatcaatga gtatcttcga
     4741 caaacgcaaa aacaggaaat ggggatcgca gagcagattt atttaacgat tcatattcaa
     4801 agagtagtaa gtgagaaaaa aagcatccaa tcataaggat tgttactgta tggcaggcaa
     4861 aacctggatt gaaaaaatag gttcacgaat gtttgtgact gtttttttca atctgggttt
     4921 ttatttttta aataaaaaag aaagaaggaa aaaacaatgg ataatcaagc agttggtcgt
     4981 cgtgtatggg aagcggtagg aggacaaaag aatgtcaaaa gtttagtaca ttgtgctacg
     5041 cggttgcgat tcagattaaa agatgaatct ttagcagata cgcaaaagtt aaaagaagac
     5101 ccggacgtga tccaagtcgt acaaagtggc ggtcagtatc aagtagtgat tggcagtaat
     5161 gtagcagatg tttatcaatc gatcgtggat gaggaaggtc ttgcagtttc ggaggatgga
     5221 aaagaagagt ccaaaaatat tttgaatcgt ctgatcgata ttatctctag tatatttaca
     5281 ccttttctag gggcgatggc agccgccggg gtattgaaag gattcttatc attggcaacg
     5341 gtgatgggct ggctgacagc tgatagcggt acttatcaaa tattgtttgc tgcagcagat
     5401 ggtgtcttta ccttcttacc tgtcatgctt gcttttacag cagctaaaaa gttcaaagcc
     5461 aaccagtttt tagcagtagc aattgctatg gcacttgtgt atccagctat cacagcagtt
     5521 gctggtgcgg gagaagctat cagtttcttt gggatcccag tgattctttc accatctggt
     5581 tatacttctt ctgtcatacc aatcattcta gccgtatggg ttcaaagtaa attagagcca
     5641 ttcgtaaaaa aagtgattcc gcaatttctg caaatgattt tagttccgct tgtcgtttta
     5701 gtagtgatgg ttccattaac atttcttgct ttaggaccaa tcgggacggt tgccggtaat
     5761 gctttaggcg gtctcttcaa ctcgatttat ggatttagtc caatcgtagc aggtctaatc
     5821 atgggaagtt tatggcaagt attcgtgatg ttcggcatgc attggggatt cgtaccgatc
     5881 atgtttttga atatcgaaca atatgggttt gatgtattga tgccaatgct tcttccagcc
     5941 atcttggcac aaggtggggc tgctttagct gtagcattgc gaacaaaaga tacgaaactt
     6001 cgagcattag ggatttcttc tacagtgact tcgttatttg gaatcactga accaactgtg
     6061 tatggtgtga cattaccttt gaagaaacca ttcattgccg cttgtatttc tggaggtatt
     6121 ggtggagcta ttatcggatt ttccggagta aaagctttct ccagcagttt ggtcagctta
     6181 ttgacgattc cgactttcat taatacggtg gatggtgtag agtcaaatgt gacagtagca
     6241 gtaatagcta caggaattgc ttttgttctt gcattcgtag gtacattgat tttaggattt
     6301 gacgaacaaa cacaagacaa tcaattagaa aacaaacatg cgaatgcagg agaaccgatt
     6361 acttcagcac ggcatacctt aaaaagtcct ttaacgggta aagtactgcc attgagtgaa
     6421 gtaccagacc aagttttttc ttctggcgtg atgggaaaag gtatcgctat tgatccagaa
     6481 gtaggagaac tagttgcacc tgcagacgga gagatcacga ctatttttcc aactggacat
     6541 gctgtaggga tcacaacgac tgacggagcg gagattttga ttcatatcgg gatggataca
     6601 gtagaattaa atgggaatgg ctttgagatc ttagtcaaac aaggggatct cgtcaaagca
     6661 ggagatttat tgattcgttt tgacatcgaa gcaattagag cagctggcta tagcgtaatc
     6721 actccagttg tcattacgaa tactgatgca tttgccgata tacttgaatt agaccaaaaa
     6781 gagattattg caaacgaaga tgttctggca attgtaaaat aaggaggaaa tcaggatgca
     6841 atcacgtttt tcagaaaaat ttttatgggg tggagcaact gctgccaaat caattagaag
     6901 gggcttatga tcgagacgga aaaggactat ccgttgcaga tgctatgcct ggaggtaagc
     6961 agcgctttgc gattatcggc agtgaagaat ttgactggac gattgatcaa gaaaaatata
     7021 tttacccgaa tcatcgagga atcgatcatt acgatcgttt caaagaagat cttgccttgt
     7081 ttgcggaaat gggattcaaa tgttatcgtt tttcgattgc ctggacacga atttttccaa
     7141 atggagacga aggtactcct aacgaagcgg gattagaatt ttatgatcag ctgattgatg
     7201 agtgcttaaa atatgatatt gaacctgtga tcacgatttc tcattacgaa atgcctcttc
     7261 atttggcaaa agaatacggt ggctggaaaa atcgaaaact gatcgacttc tatgaacgat
     7321 ttgctcagac agtattagag agatacagca gtaaagtcaa atattggatg actttcaatg
     7381 aaatcaattc ggcctttcac tttccagctt taagtcaagg attagtaaaa agcaatggag
     7441 caggagagta ccaaaatatt ttccaagcat ggcataatca attcgttgca agtagcaaag
     7501 ccgtaaaaat cggccacgaa ttgcgttcgg atatccaaat cggctgcatg attatctatg
     7561 caacaactta cagcatcgat gccaatccag tcaatcaggc agcaacaatg atccaaaacc
     7621 aagaattcaa ttttttctgc acggatgtcc aagtacgtgg agaataccct gcatacacag
     7681 cgcgtaccca taaaaaatat ggagtcgatc cggaaaaatt agagcaaacg gaaaaagact
     7741 tccagttgct aaaagaatat ccagtggact acatcggatt tagttattat atgtctacgg
     7801 caatcaatga gactgatcca caagcagcaa cttcagaagg gaacttgctg ggaggagtaa
     7861 aaaatccgtt tcttgaggca agtgaatggg gctggcagat cgatccagaa gggctgagaa
     7921 tcgcattgaa tgaattatac aatcgttacc agaaaccatt attcattgtt gaaaacggtc
     7981 ttggagccat tgatcatatg gaagaggatg gcacgattat tgatgattat cggatcgatt
     8041 atttacgtcg tcatatcgaa gcgatggcta atgcagtagc agacggtgta gatttgatgg
     8101 gctatacgcc ttggggatgt atcgatcttg tcagtgcatc aacaggagaa atgagtaaac
     8161 gttacggctt tatctatgtg gatctagacg atgaaggaaa tgggacactg aatcgatcaa
     8221 agaaaaaatc ttttgattgg tataaagaag taattgcctc aaatggcgta aacttgtaat
     8281 taaagcagct gaatgaaaaa gaacacactt ctcacatgtg atttgtgttc tttttttatt
     8341 ttttatgtgc tattctttta gtaatagtaa agcaaagggt gacgttatga aaaggacacg
     8401 tgtactatac ttggaagtcg cagataaaat taaagaagat attttctcag gaaaatatcc
     8461 agtcggttcg atgttgccga cagaaagtga attagaagaa ctgtttcaag tcagcaaaat
     8521 cactgtccga aaggcaatcg aactgctggc atcagatgaa tatgtcgcaa agaaaagtgg
     8581 tcgtgggacg acggttctaa gcaatcgtcc ttacaatcga ttatccaaag cggcaagctt
     8641 tacgcaaatc ttggagaact cccgatatga agtccggaaa gaaacgctgg atttgcaatt
     8701 ggtccatata aagaaggaag atcctttgtt ttcctttttc ggtgagcaag ctgtttgttt
     8761 taagcgctta tattatttga acaatcagcc ttatatttat tttgttcatt atcttccaca
     8821 agaaatgatg caattcagca gagaagaatt tggcaaagaa tctttgtatc gtttgctgac
     8881 gaaaaaagga tatactattg actcattttc tgatgatttc tcggcagtat ttttaacaga
     8941 agaagaacaa ggaattctga aaacagacgc acagatcggc atcaaacgga tacggaaatc
     9001 gtctacgcaa attggggaaa tgattgaata ttcggaggcg gtctaccata cggagctgca
     9061 tccttaccat attgattatg aaacataaat aaaaaaagag gttgtgaaaa agctgccctg
     9121 catcaagtaa taagaacagc caaacaaaaa tagcttctca tatttttcgt ttggttaggc
     9181 ttattaccgc agatacacgg ctgtttctgg attaccattt attctggata agggggtgtg
     9241 gcacgatttt cagaaggaat ctatgaacca atcactgtat tgattttagc ttcgatggta
     9301 accccattcg gcttgacgat cgcttatttc ttagggaaaa tcattcgtaa aaatatactc
     9361 aatcgtcaag aaatcgatac attgaagacc gcttttccta tggggatctg tcagatcaca
     9421 gagggatgtt tccctatcgt attgaacgac ttggcacgca atgtcatagc aacgggagtc
     9481 ggtgggacag taggtggcgg attaagcatg ttttggggcg cagatagccg tattccggca
     9541 tcgggaatgt ttgcagtcgc tacgatgacc agaccatggg cattcattgg tgcattgtta
     9601 gctggttctt ttgtcacagg aataaccatt ttattattga aaaaaccagt agatccgaat
     9661 gcagaaatca tccaagaaga aaaagaggaa gaagacatat cttgggatga tttgacaatt
     9721 agttaggaga ggcagaatgg attacaagaa aaaagtacaa tagatgtacc ttgagacagg
     9781 aatggtatga aagaatcaga gttagaaaga atcgaatata cagattttgg tttaggagcg
     9841 atcgaaactg tccagagcat cggcaccctc cttgaaatga cgatgaagga aaaatgagaa
     9901 cctttcgttg tcgaaagggt ctggtatacc ttatgtcgaa ggaaaggaaa caaaaaaact
     9961 ttcctgtact attccaaaaa ggaaagaaag ttattataca gtaaaaaaag aaattattct
    10021 tcatgctaga gatcagtaca cgttcgagcc caatatcaaa cactagttcc aggcaggcgc
    10081 aacaggtgcg gttatcactg aattttcttc cagcagtgac gattcaagtg atatttttac
    10141 aaatccagct atttatcgtt aacaaaaaga tttttaggag aagctgatga acaaaaatga
    10201 tcaaattttg atcgtttagt tcgtcggctt ttttatttag agaaacatca tttatcagtg
    10261 aaacataaca cgagaacaaa atgatttttt cgttaataaa agaaacaagt atttgttgtt
    10321 taaaataata cacgttattg gcatatagga cgaaatccca gtatcttcta taagtaagtc
    10381 atttcatatc gatgaaaaca cgttatttat ttttgtttta tctataaaat ggctgaaaaa
    10441 tgattttttt ctaaatacta tatatagtgg cgtctatcct atataccact aaaaaactac
    10501 aaaatctagt cttttatttt aaaaaaacac gtgtagaatg aagatagact tttgagttag
    10561 ggagtgtttt tttatgaaac ttactgtttt ttgcggttcc cgttttggta ataaagaaag
    10621 ttacaaattt attgcgcaaa cgttaggaaa atacatggca caagaagaaa tcgaactggt
    10681 ttacggtggt tcatactccg ggattatggg agtgattagt aaaacagtac tggaaaataa
    10741 cggaaaagtc acaggtatct atccgaatgg gctttttgaa actgaattgc ctggaaaaga
    10801 tgttactaca tttattccta cagaaacgat cgatgaaaga aaggtattat tatttgaaaa
    10861 gggagatgct gtcctcgttt tccctggagg tctaggtaca ttggaggaat tctcacaact
    10921 tctttcttgg atagcaatcg gtctgacgcc cgacaagcca atcggaatat tgaatattgg
    10981 cggttattac agcggcttgc aacaattatt agaaactttt gcaaaagagg attttatgga
    11041 taaaaaatgg ttggatcaag taatcttttc caacaatcca ttggaactgg tcaatgaatt
    11101 acgagcagta tctattaaaa atcagttatt attgaaggag gcaaaataat gagtcaaata
    11161 tatttagcag gaccattttt ttcagaggag caaatcgatc gagtaagtcg tatcgaaaaa
    11221 gcgttggagg aaaacaaaac agtcacaagt ttctactctc cacgtcatca ccaagaaagc
    11281 aattatgaac tattcagcgc tggatgggcg caggaagtat acgaaaaaga tatggaagaa
    11341 ctgacaaatg cagaatttgt tgttgctatc cttgactttg agcatcaaac aatcgatcca
    11401 ggaacggctt acgaattagg tgtggcaact atgttgaaaa aaccgatgat cattgtacaa
    11461 gaagagacgg tacctacgaa cttgatgatt acacaaagtc tccatacgta tttaaaatca
    11521 gaccaagctg tacgtgaata tgattttgaa acattgcctg ttgaaactta cgtaggcgag
    11581 tacttataga agaggaaggg ttgtatgtca gtgattttga tttcaggaac gattggtgca
    11641 ggtaagagca gtttgacgga tatgcttgca aaagagattg attctaaacc attttatgaa
    11701 aatgtcgaag ataacgaagt attgccgctc ttttattcaa atcctgagca gtacgctttt
    11761 ttattgcaaa tcttcttttt gaacaagcga tttttagcaa tgaaaaatgc tttagtgaac
    11821 gatgacaatg tgctagaccg atccatctat gaagatagcc tgttgttcca tctaaacgct
    11881 gatttaggtc gggtaacgga tatcgaagta cagcagtacg atagcttatt agatacgatg
    11941 ctaaacgagt tagatgatgt tgctccaaaa aaacggcctg atttgatggt ccatatcaaa
    12001 gtttctttgg acacgatgct agaacgtatc aaaaaacgtg ggcgtgacta tgaacagcta
    12061 gaaagcgatg agacgttata tacctattat gaaacgttga acacccgcta taatcaatgg
    12121 tatgaagatt ttgatgtatg tcctaaaata caagtggatg gggataaatt cgattttgta
    12181 gaaaatccag aagatgcaaa aaaggtgatc cgtcagatcc aagaaaaatt agctgaattg
    12241 gaaggaaagc ctgctggaaa atattttacg gataaccagc atgcaaaagg tcaagaatta
    12301 ggctaaaaat aaaaaaccaa acgacgggaa agaaaaatt
//
LOCUS       DANKKG010000079        12291 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000079 DANKKG010000000
VERSION     DANKKG010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.079"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..462
                     /locus_tag="PW004_001816"
     CDS             <1..462
                     /locus_tag="PW004_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HDL2726784.1"
                     /translation="TLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTTLFVS
                     VIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGLLPKF
                     MKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            471..698
                     /locus_tag="PW004_001817"
     CDS             471..698
                     /locus_tag="PW004_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2726785.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            1334..1705
                     /locus_tag="PW004_001818"
     CDS             1334..1705
                     /locus_tag="PW004_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726786.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(1961..2203)
                     /locus_tag="PW004_001819"
     CDS             complement(1961..2203)
                     /locus_tag="PW004_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDL2726787.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(2235..3137)
                     /locus_tag="PW004_001820"
     CDS             complement(2235..3137)
                     /locus_tag="PW004_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDL2726788.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(3150..3338)
                     /locus_tag="PW004_001821"
     CDS             complement(3150..3338)
                     /locus_tag="PW004_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HDL2726789.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(3352..3915)
                     /gene="amaP"
                     /locus_tag="PW004_001822"
     CDS             complement(3352..3915)
                     /gene="amaP"
                     /locus_tag="PW004_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HDL2726790.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(3953..4846)
                     /locus_tag="PW004_001823"
     CDS             complement(3953..4846)
                     /locus_tag="PW004_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL2726791.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(4924..5847)
                     /locus_tag="PW004_001824"
     CDS             complement(4924..5847)
                     /locus_tag="PW004_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDL2726792.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(5896..6246)
                     /locus_tag="PW004_001825"
     CDS             complement(5896..6246)
                     /locus_tag="PW004_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HDL2726793.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(6279..7181)
                     /locus_tag="PW004_001826"
     CDS             complement(6279..7181)
                     /locus_tag="PW004_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HDL2726794.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(7174..8031)
                     /locus_tag="PW004_001827"
     CDS             complement(7174..8031)
                     /locus_tag="PW004_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HDL2726795.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            8365..9174
                     /locus_tag="PW004_001828"
     CDS             8365..9174
                     /locus_tag="PW004_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL2726796.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            9214..9711
                     /locus_tag="PW004_001829"
     CDS             9214..9711
                     /locus_tag="PW004_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL2726797.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(10096..10169)
                     /locus_tag="PW004_001830"
     tRNA            complement(10096..10169)
                     /locus_tag="PW004_001830"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10133..10135),aa:Ile,seq:cat)
     gene            10356..10679
                     /locus_tag="PW004_001831"
     CDS             10356..10679
                     /locus_tag="PW004_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726798.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            10843..11097
                     /locus_tag="PW004_001832"
     CDS             10843..11097
                     /locus_tag="PW004_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726799.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            11167..11412
                     /locus_tag="PW004_001833"
     CDS             11167..11412
                     /locus_tag="PW004_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2726800.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            11488..11853
                     /locus_tag="PW004_001834"
     CDS             11488..11853
                     /locus_tag="PW004_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726801.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
BASE COUNT         3520 a         2303 c         2111 g         4357 t
ORIGIN      
        1 acattgctat taaaatttgt cgttttaggt ataagtgccg tatttatttt gttcggcgta
       61 cttttttata ctcagatcgc tacaagcgaa aaaggattca attttgattg gcagaccaca
      121 ttatttgttt ctgtcattta ctttgccctt ctcctaggga tcgttattgc ttatttctta
      181 ttgaaactat tgaaaaaaat cgaagataac caagcttttt ccactcaaag cttaaacata
      241 ctaaaaaaaa tcgaacgaac cattttgttt attggtttcg ccttttttgg cttacttcct
      301 aaatttatga aagtagcgga tttagacgat gctccaggtt tgatgttggt aggaatcgct
      361 gtcgtattca ttccttttgc agtatacaca ttaacaagcg tgctggaaaa actgcttgaa
      421 catgcgattt tacttaagac agatcatgat ttgacagtgt gaggtgcgac atggcaatta
      481 tcgttaattt agatgtagta ttagctcaaa aaaaaatgag tgtgactgaa ttgagtgaga
      541 aagtgggcat tacgatggca aacatctcta ttctaaaaaa tggaaaagca aaagccatcc
      601 gtttttccac tctcgaaaaa atctgccaag ttttggagtg tcaaccaggt gatttgttga
      661 cctataaaaa tgactcgaat actataccag aagaataaaa taaagtagaa caaacagtaa
      721 ataaaaaata atctgaatga tagatgtgtg agacttttag aagttattta cgtctattat
      781 tcagattttt tatttcgatt gaataccgtt ttatctataa ataagatatg tactgactca
      841 tacaaagggg gccttttctt ctccatgtac tatcactttt cctgatacaa ctggtttcct
      901 gttacgttgt agttggatct gcttttagta taatacttta ttcattccac cagactgata
      961 gcagtttgag cagtctaaat aatcatgtag tattacaggc cattctgttt cataccttga
     1021 agtgcttaaa acttcagcta atggcccatt gtgttaactc ttactccatt cttttttagt
     1081 aagctcccat atatactgta aagccttcta tatattatgt catgttgctt tttaacattt
     1141 actatgtagt tttgataaaa tgatttgaat aacagtgatg gacaattctt ttttattttt
     1201 aacattattt tgataataat gattatttat ttaaaaatac attagataac atttgttacg
     1261 acacgaaaaa gcttcacaaa caaatatcgt tattttaaaa tgagggcgaa ctcacacaat
     1321 taggaggaat agtatgccat ttattgttta cttttttctt tcattactcg tgatctatat
     1381 tccattacca acatttttcc tgctcgtcaa tcgtctagca gagcaacatg atacgattgc
     1441 tgctcttacg aaactttgtt tacttctatt tgtttgtcta gattacttta cgagctatta
     1501 ttggctaaaa cagacaagag taaaaaagag atattatttc ttccttgtct ttttcaatct
     1561 ctttgagttt ctgatccatt gctttttact tcatcagtat catacgatcc atttgtggat
     1621 catctgggtt ttcaaagtgg tcgtattatt ttgtatgttc acctttccta tctcaagaaa
     1681 atttagggag gcattgtttt attaaagaag cttctctgta tcaataaaga cacatttaag
     1741 gaaaaacttt taaaaatcta attgggaatt tgcccgcata aataagaggc tgtgacaaaa
     1801 gtcttgtcac agcctcttat tccgtataaa cagtgttcaa aagcttgcat cttcgataat
     1861 atgcccaacc aaatgaaaaa tatgagaagc tatttttgtt tggctgttct tattacttga
     1921 tgaagaacag ctttttcacg acctcttttt aaaagtcttt ttacagtttt cgagcaaaga
     1981 atgatacgat tagtactaaa atgatggcac caatcaatga tggcacgatt gccattcctg
     2041 caatacttgg tccccaagaa cctaataatg cttgaccaag tgctgatcca actaaacctg
     2101 ctacgacatt tgcaataatc cccatcgaag aacctttatt cgtgattgct cctgcaattg
     2161 ctccgataat tcctccgacg ataagtgacc aaataaatcc catgtttcgt tcctcctaat
     2221 gttttacttc cttattactg aacccgtggt tctgtatttt cttttacttt ttgtgcgccg
     2281 cttccagctg ctgattttac tttatctgtt tgattcgaag caaattcacc ggtttttttc
     2341 gctgcgtcag ttactttgtc ttgcaccgtt tcttgatctt tttcaaattc ttcttttgtt
     2401 ttgatgtcca ctacgttagc gttgacttcg atcacttcta aatgtgtcat attttttact
     2461 tctttgccga ttacttcttg catttcttca aagatttttt cgatatcttt gccatattcc
     2521 acaatgacat ccatatcaac tgctacttgt tttttaccga cttctgtttc gatcccagcc
     2581 gttacattat ctgtattcac aagttttcct gccacattag agaagaaacc accgtttgca
     2641 gataataaac cgtctacttg ttcaattgcg attccgatga ttttttgcac aactttatct
     2701 tcataattca gctcaccgcg cattgaatct tcctgttcag aatcatgctt tccttggtta
     2761 tttgtatgat cagaaccaag ttttccttgt ccgtttgtgt ggtcagaact aagtttttct
     2821 tgtgctttcg catggtcaga aggatgtttt tgctcttcca caggtttaga aggatcgatt
     2881 gcatcatgtt gttctggaac tttagattct ggagttcctg gatgatgagg tgcatgcttt
     2941 tctgcatcat gtttcttctc ttctactcct tgttgaacgt tagggactac tggatctgga
     3001 tgtgcagata ctggtgctgt tttgttcttt tcatcgttca caccatttga ttggtttggt
     3061 gtcgttgggt ctggatgtgc agacactggt ttttggttgt tcattttatt gttattattt
     3121 ttgttattgt tattcatgga tgtttcctcc taagatttaa aatagtgatc taagattttc
     3181 gtttgtttca gataaaaacc gatagcgacg ccgatcgctg ccatgatcaa gatcaataag
     3241 gtcttgaaaa agcccacggt gatccataaa acggctaata tcaatccaag taccccaaag
     3301 atgataggga gcttgtatgt cgaaaatagt tctttcattt ttggtccccc cctattctac
     3361 tcgtttgcgg tcattgcttc gtttagctgg ttcttcaaag cctgtgtatt tcaccgatac
     3421 tttcactggc tcttttgttc ccaatatgtg ttgtacttca ttttcgattt ctttcatcaa
     3481 tgttcctgtt ttaccaatga gtgctgaagt tcgtttcaac tcccctttta cattgacggc
     3541 gatcttgttt ttcgttgctt tgacatttac ttttggtgaa tggatgaatt catcttcatg
     3601 caagtgagaa cgaaccattc cttcaattgc ttttttatcc aacgtcaact ccccatcacc
     3661 atgtttcagt agaaagtttc cgattgtttt tggataaaac aggaaaacaa gtaacgtgat
     3721 aattgtcaaa atagcaaatg caacggcaag ccaaaataca tattgttgca cgtatgaatt
     3781 agtgatggaa aagaatcgaa acttctctag ctgaaaaggc aatggatagg ttgcacttaa
     3841 tagtatcgca aacaaaagca cagatagcaa aagtaatgtt ccgatcactc cgatcgtttt
     3901 tacaccttta ttcattttcg cacctcctca taggggtgaa acgatcaact tcttataagt
     3961 tgatcgcttc tccaccagtt actccgtaaa tttgggctgt aacataactt gctttattcg
     4021 atgctaagaa aacataaact ggtgctaatt ctcctggctg acctgcacgt tctagtaaac
     4081 tttgctgacc aaattcaggg atcgaatcag aaggttgtcc ttcgtctagt tgtaatggtg
     4141 tccagattgg accaggtgct acaccattta cacggattcc tttaggtgca agctgttttg
     4201 ctaaaccgat cgtaaagtta gcgattgaag cttttgttgc tgcgtaatct aataaatgtt
     4261 cgcttggatt gaatgcttga acagaagttg ttgttacgat tgctcctcct gctgggatat
     4321 gcggaacagc ttctttcact aaagcaaaca tagcgatgat gtttatcata aatgtgtctt
     4381 tgacttgctc aagtggtaac tcagcaatag actgacaaga aatttgctgt cctgcgttta
     4441 gtaccatcgt atctagcccg ccaagttcac tcacggcttt atctatgatt tctttaggtg
     4501 cttgttcgtc acgaagatca aaaggtagta agacaatctt acgtcctgca tctttcacgt
     4561 attttgctac ttcctctgca tctttttctt ctcctgggaa gaattgaaga gcgatatctg
     4621 ccccttctct agcaaatgca atcgctgctg cacgaccgat ccctgaatct ccaccagtga
     4681 ttagtacacg gcggttttct agttgattat tgccttcata ggattcttct ccacaatcag
     4741 gaacaggaat catattctgc tgtaaagctg gtgtatcctg atcttgttta ggaaatttct
     4801 ccgtatggta caagtttcgc gggtcgatta attttggttc tgtcattgct gtattcctcc
     4861 tagtttttaa attgtattgc caaaacaaat ttctttgttt gatacctgtt ttaaatagat
     4921 tctttaattt gtataatctt tccggtataa atggtaacct aaaactactg ctgctatcaa
     4981 agagccaaag actacgagta taaagcctag agcagaactt ttacccggta atcctcctgt
     5041 attcattccc caaatccctg cgaacaacgc cggaacggaa atgattaatg cagctgaatc
     5101 tagatatttc atgatatgat ttagattatt gtccatcatc ccgttgaata gatcaccaat
     5161 actctctaat aaatctcgat agatctcgat cattttttct gtttgtcgct gtctcaaatg
     5221 gacatcatag agtagctgtt catcgtctaa acgtttgatg aaagtttcag aattccataa
     5281 ttgatccatt gtttcccgtt gatctcttaa ggtatgatcg atatatacga tgtctctttc
     5341 aagatctgcc tgtttataca gttcttcatt ttccgttgtt ttacgagctg ctttatccaa
     5401 agcgtcaatt cgctttttca tctctcttag ttctttcacg taatgggtgt aaatatctaa
     5461 taatgtatac gtgatgatct tttcaaacga gtttaaatga tttccatgct ttgtcagcag
     5521 ccgatccaca aattcggttt ttctatcgat acaagtaatc aagagattat cggaaagtac
     5581 aaaagatatt ggcgttaatt ttttttcgat acgattatct gactgatcta gattaatcac
     5641 taccaaagaa aatggatgct ctagtttcgt ctcataaagg cgctccatcc gagaaacttc
     5701 ttccggatat tcggttccta cgaaaatatc atcaggaagc tcgaattctt cgattgcttg
     5761 ctcaatctct tcgggctttg cactatcaag tacgagccaa ttatattttt tttcatctgt
     5821 ttctattaca ttcttttctt catccacaaa gtagtatctc aatgtatacc tcctttttgg
     5881 actttctcga aactattagc tgaagaagca gttctttaat ttgatattat aatcgagtac
     5941 tgcgatttca ttttctaatg aaaagctgtc aagctgtttg ttgaagtcca tgttcaatcg
     6001 tttaccgaaa taattttgct ctaatttaga tgtatagatg tccaataatg gatgctcgat
     6061 tttagtcgca tattctccaa attccgaaac aaaaggaatg atttgaaaaa agctgccttt
     6121 tgcaaaagca cctttgtccc ttagaaatgg atcggaatca ttgataagcg cgagtgcgat
     6181 ttttgtattt tccccaacct tttcaattaa cgtctctact gcatttttag acattgtaat
     6241 ttccattcct actccccctc ttcagttgtg gtcttctatt atttaacaac tacatctgtt
     6301 tttggttgaa cttttgcata ttttggatat ttagggaatt tagctacaca acggaaagcc
     6361 gtcatccctg catatttttc gtaatatttc ttcgttggcc cttcaaaatc cttgctccac
     6421 ggtttcacat gtcctgaaaa atggataatg taaggattgt tgcgagtttc ttcgtattca
     6481 cgttcacctg ctgctgttgg atgttttttt gcttttgcca tgatatagcc ttgtgcattc
     6541 caacgcggat gaagtggtaa ccaacggtca tgtaagatag catttaatgc atcttggtca
     6601 tggaatcgta atttttctgg attatgttcg atgaaatcta atactttttg cgtaatattt
     6661 tcatctaacc attttttcac attgatcagc atcaaacctg agttgaaata acgcattgat
     6721 ttagctgaaa ttttcatttt ttccagtcgt tgatggaacc cagcgtcttc aacagctgct
     6781 acgattgaat caccgaagtc aagacgccat aatttagaga tatcttgtaa agcgatcatg
     6841 tcgcaatcca tatataagat acgttcaact tctgtgccgc ggaacaattc cggaatcgca
     6901 atacgataat aagctgtttc tggaatacga tcgctgatta ccacatttgt aaagaattct
     6961 ttattgattt tcaagaattc cacatctgag ttcattcgtg cgtttttcac tgagaagcgt
     7021 aaaagctgtt tactttcaaa atcgatatca tcgtcaatca catagaaccg aacaaattta
     7081 gacgggttgc aattatctaa tactgagaca aataatgcag ctaaatgtgg tacgaatttt
     7141 gtgttacaac ttgcgactac tgctatttct tttttattca ttgatctcgc ctactttcgt
     7201 tagagtactg tgtgctaatt tttttaagta taaattggta tacggatgat ctttcaacgt
     7261 attccaaggt ttatcatgtc ctgtaaagtg aacgatcgaa ggtttttcat ttccttcttt
     7321 atactgacgt tcatattttt catttggtgc tggatgacgt tcaaaaatca gcgaagtctg
     7381 catgttccat tttggatgaa gttgttccca atcttcataa agtacagcat tcaaagcatc
     7441 ttggtcgtga tagatgattc gatcgccatt ttcactgagg taatggatag ttttttctgt
     7501 aatttctttt ttattccatt gatcaatgtc aatcaccatc actcctgaat tgaagtacaa
     7561 gtcatcggaa tcgattccta atctttccaa agctttcact tggcctggat cgataatagc
     7621 gccgattgtt tttccattta acggctcatc atatagtttt acgatatcgt ctagtactaa
     7681 cacatctgaa tctaagtaaa ggactttctt ataatcctct ttagctaata agtttggtaa
     7741 agaaattctt aagtaagcgg tagttgtgat atggtcactg actaggaagt cttcgtagat
     7801 atctttttcc actgtcagga attggatact tgcattactt gaatatttat ttaccgtttc
     7861 ttccaatcct tctttactat attctgatag accatcgtca atcacataaa attttatgcg
     7921 tcttgtttta ttgcaatttt ctaatgcagt tgcgatcatc acgctcaagt aaggagcata
     7981 attttcgtct gaagcagtta ccacaggaac tacgccgtat ctcttctcca tcaaaatcac
     8041 tcctttttct aatctttttc taacctgttc tacatctaga atattcctca tttggtttta
     8101 aagcaaaata tatgctttgt tttcattgaa tattttaaaa tttttttatc acaaaacgca
     8161 tcggtaagcg ctaactattc gtttttcatg gctttttatt caaaggtatt gaccaaaaac
     8221 tactatatat agccaccttt ttcttaagaa tgttttttga aaaaattcat aatgacaaag
     8281 caagttagtt tcattttttt aaattctcat ttaatcttaa ggtaacagga aaatagatat
     8341 gtaaaaagaa agtaggaaga taaaatgaaa ctagcagcga ttgatttaga cggtaccttg
     8401 cttgatcgaa atggaaagat accagaagaa aatatccaag cacttcggaa atttgatgaa
     8461 aatggcggaa tcgtaaccgt cgctaccgga aggaacagca tttctgctaa agatattttt
     8521 tccgaattag gaatcggtgg ctatttgatt tcttccaatg gttcactgat tgctgaaatg
     8581 aaggatggaa aaatggcgaa agttctcaga cgttcaaaaa tagatagcga tacactgaga
     8641 aaagccttct tctatgctca agaagcgaaa atatccatca ttgctagccg agaaacccaa
     8701 gatgatcagc tgacattcga tgaatcaaat ctagtcaaag atgaccctta ttatgaacat
     8761 ttcaatttac agaaccattc ttttgatcaa attttaaata tgttggaaga tccttctctt
     8821 agctatctta aattagcatt aacagataag agtgaagaaa aactacagaa gctacaaaga
     8881 gatctgcaag cagatggtat ccaatcagta ttctctgatc cgcatttctt agaaatcacg
     8941 cctacaggta tcactaaagc tcattcactt gtttttctgg cggattacct tggaatcgaa
     9001 tcgagtgaca tcatggcttt tggcgatcaa gaaaacgaca ttgctatgtt gaattacagc
     9061 gggttgagta ttgcgatggg gaatgcacag cgacaagtaa aagaaattgc aaaccaagta
     9121 acactgacga atgaagaagc tggtgttgct tatattttaa aaaacttcta ttaaaaagaa
     9181 taaaatgacg atatgaatgt aggaggaaag aaaatgttag ttattattga tatgcaaaat
     9241 catattcttg atccaaacag cgaattttat attccagagt cagatcaatt ggcagaacgt
     9301 attgctcaaa gattagctaa agcacgccaa aataatgaat atattctctt cacacgggat
     9361 ataccagttg aactgaagga caaagaagaa gaacgagaag atttgcaatt gatccctctt
     9421 ctttctccac aagaaaatga acgagtgatt aaaaagtatt actttacgat tccacctgaa
     9481 actttagctg aaatccagga aactttattt gaaagcaaag aagaacaaaa agagatcgaa
     9541 gtagtcggga ttgaaacaaa cttgtgtgta ctctctaatc tgataggcct gcagagtgct
     9601 tttcctgaag cagacttttt tgttgatcca atgttagtct ccagtagaaa acacggagaa
     9661 tccgctttag agctgttgaa agatttcaac gtctctatcg tcgaatcata aaaatatcta
     9721 tcttcattta cgatatttaa cacacaataa aaaaagcacg cagaactgtt ttagtcctac
     9781 gtgctttttc tatcaaagta gattggcttt tttatgacac agagtgaaac ggcaggctca
     9841 cgacctcttt cttaaatggt gttcaatcag ctgaccctcg gtattagctt aacaaacggc
     9901 aaaatatgag gaactatttt tccgtttgtt aagctaatac tcaggtcaaa acgctgattt
     9961 cacaacctct tccttaaacg gtattcaaaa gcttgcatct gcagtaataa gaacagccaa
    10021 gtaaaaacat aagaagctgt ttttacttgg ctgttcttat tacttgatgc agggcagctt
    10081 tttcacaacc tcttatgtac cttgtaggac tcgaacctac gaccggacgg ttatgagccg
    10141 tctgctctaa ccaactgagc taaaggtaca tcataaataa aatttatttt tgttactttt
    10201 caactatagc acgaatgaca aactctcaca agtttcaaaa tataagtaaa ataatattgt
    10261 tattcttatt tcttttactt gtgtcataac tattttgtta ctttaatttt tatttgctta
    10321 ttttagaaaa aatgttattc ttttatttat tctcaatgtt aggagtgatt attttgccat
    10381 ttattgcgat tttattcgat ttagttgcgg cagcagctta ttttctccaa ttaaattatc
    10441 aaacctcagg aattctgttt attggaatga tttttcaagg tgtgattacc cttttgcttc
    10501 ttgtcatgat gctcagctat aaaggaaaaa gatatgcccg tattcaaaca aaagtatttg
    10561 ttaaatatgt ttccattcgc tatgggatca tcattctttc ctttttagtg aatgcgatcg
    10621 tcctcttttt atatgtattg aattacttag atatcaatcc attgattttt tctcgataga
    10681 taagacacat tcagcggaac agttcctgct tatcagcaga aaactgttct gctttttcat
    10741 ttttcattga tctattcttc ttttattttc ctagcatttc atttgtgatg tatgcaataa
    10801 catgttatct tttattatat gaatcgttga ggagtccgtt acatgattac gttggtgaga
    10861 gttttgtttt gggtgcctgc ggtcgcactg gtcgcaagta ttgtttatct tatgaattgg
    10921 aacaaagaac ggttttattt agctattttg actcttccgg ctatctactt tatgtggaaa
    10981 gtttttaatt acaactactt tgaacccgat agtgttttta tagaagaatt atcaggactg
    11041 gttttgtctt tattgatcgt cattctttat ttgatacgtt taaataaaaa acattgagta
    11101 ctattgattg catgtgtagc tttctatagt cattataact attctactat aatgaaaggt
    11161 gattaaatga gtatcgaaga agctatttta ctggatactt tacgttgtcg tctgaatttc
    11221 ttaagaactg gccgatcttt atctcaagta gctgtttcta gcgcgttaag tattccttta
    11281 ttcaaatatc gtaagtacga agaaggagaa gaaattccaa gcatcattga attaatctat
    11341 atcgctgact tttacgatat ttccgttgat tacctaattg gtcgctcaga aacagcaagc
    11401 aaactgaagt gattttttcc agccttggtg ctggtttttt catttaacgt ctaaggttaa
    11461 caatctatat ataaaggaga catttctatg cgattatggc atgaaaaatt gatttcaaag
    11521 cttcctcgtc aacagctttt aggacaacat cgggaatgct gtgcattaag aggaaatggc
    11581 tggggaaaga aacatgctac tgtggattat gtttttcact attctcctta tagattattc
    11641 caatatcatc agcttgtcat ggatgaaatg gaaaaacgtg gttacagccc agcaccagag
    11701 tggaaaaatc ctatgtatcg tggcaaatca tgcgaagcat tttttgattt acctactgta
    11761 gagcgcaacg tgccgattta tccggaacac gatgaagttt atctagcaga atgtttagca
    11821 aatttggagc aaaaaggaat ttatcttacc taatataact agaaaagcct tctatctcct
    11881 cttacttcat taaaaagctt taaccatctt agatcaagct aaaatgtcgt aagttttagg
    11941 gttatcttca tcctcacgtg caaaactaaa agcgtactct agttttggtt catcgccaac
    12001 ttccctagac ataaatgaac atctcctgac gtggtttaaa agtaccgcat cttcgacgtc
    12061 tgtatacgat gcacagcaca gattgcttct tttgatcttt cttttgattt ttctgttgct
    12121 tcttctacac cttcttcgat tactacatcg atgcttacaa ttgcttctgc ctaatattcc
    12181 catgcatcgt cgatttaagc ctctgtagtc actgtgatcg tttccacatt ttaactcctc
    12241 tgtcgtggct tcagttgatt ttgtgatcag ttcagttgct aagccagagt c
//
LOCUS       DANKKG010000080        12244 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000080 DANKKG010000000
VERSION     DANKKG010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12244
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.080"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            251..2269
                     /locus_tag="PW004_001835"
     CDS             251..2269
                     /locus_tag="PW004_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL2726802.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    2339..2545
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2696..5110
                     /locus_tag="PW004_001836"
     CDS             2696..5110
                     /locus_tag="PW004_001836"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HDL2726803.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            5458..7011
                     /locus_tag="PW004_001837"
     CDS             5458..7011
                     /locus_tag="PW004_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HDL2726804.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(7392..9176)
                     /locus_tag="PW004_001838"
     CDS             complement(7392..9176)
                     /locus_tag="PW004_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2726805.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(9191..10912)
                     /locus_tag="PW004_001839"
     CDS             complement(9191..10912)
                     /locus_tag="PW004_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2726806.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            11012..11773
                     /locus_tag="PW004_001840"
     CDS             11012..11773
                     /locus_tag="PW004_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL2726807.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4036 a         2199 c         2469 g         3540 t
ORIGIN      
        1 ataactgtaa tcggaaagac attttttggt aatgcaaatg aaatgttagg gaaataaaat
       61 cagtgttttt cgaaatcctt ttaaacaaga acgtagacgt aggaagaact tgaaaatgaa
      121 cagaagttgt tcattacggt aaattacaat atgattacaa aaaacaacaa agctcttaaa
      181 taacttttaa taaatattaa gagaagttat aattattaat aatagataaa ttgaagttgg
      241 aggatagaag atggaaaaca ttgcaagaaa agaacggaga cgtttagaac agaccaaacg
      301 ttttcgaaaa gcaaaacgag gggcagcaat cgtgggaact gcgatggtag gctgttctgt
      361 cgctgcccca ttagtacagc cggttcctgt tcaagcggat gaaacacctg ttcagttcgg
      421 cgcaagaatc aatacagcgg catttatcac agaaattgct acttatgctc aaccaatcgc
      481 tcaagccaac gatctatatg catcagtgat gatcgcacaa gcagttgtag aaagcggttg
      541 gggcagtagt gcgttatctc aagcacctta ttataatctt tttgggataa aaggcagcta
      601 tcaaggacaa accgtctaca tggatacgct tgaattttta aataatcaat gggtaaataa
      661 aaaggagcct ttccgacaat atccttcttt tgctgaatct ttcaatgaca atgcctatgt
      721 acttagaaat acttcatttg gcaacggtta ttactatgcc ggtacttgga agagcaacac
      781 gaaatcctat acagatgcga cagcttgttt gacagggcgt tatgcgactg atcctggtta
      841 cgcaggcaaa ttaaataata tcatcgcaac ttacggattg acccaatatg atacaccagc
      901 tagcggaaac gcaggcggtg gagcgactac aggtggcaat aatactggaa gtgcaggtag
      961 tactggcggt tcaagcaatt ctggtaatac aaactcaggt tcagcaactt cgaccactta
     1021 tactgtccaa tctggtgatt ctgtttgggg aatctcaaat aaattcggta tcacaatgga
     1081 tcaattgatc caatggaaca acatccaaaa caatttcatt tatccaggac aaaaactgac
     1141 aatcaaaggt agccaggaaa atggctcttc gacgaataat tccggtaaca ataccaactc
     1201 ttcaggaaat gctggtacat caaacggagg acaaacaact ggtgcgaaat acactgttca
     1261 atctggtgat tctgtttgga agatctcaaa tgatcacgga attacgatgg ctcaattgat
     1321 tgaatggaac aatatcaaga acaatttcgt ttatccagga caacagctaa tcgtatcaaa
     1381 tggcggttct tctaatgctg gagcaaatac tggctcaaat actgggaata caagtggctc
     1441 tagttcgtcc agctcaagtt cttctaatac agcttcagga acgaaatata cagttaaagc
     1501 cggagactct gtttggagcg tctcaaataa atacggaatt acgatggctc agctaatcca
     1561 atggaacaat atccaaaata actttatcta cccaggacaa caactgactg tttcaaacag
     1621 tggatcaaca aacagcacgt caggtaatac aggttcgtca accaatacaa ataacagcag
     1681 ttcagttaat actggcagca gttcgtctca aggcacttat accgtgaaag caggtgaatc
     1741 tgtttggagc gtggcaaata aaaacggcat cacgatggat cagttaatcg aatggaataa
     1801 tatcaaaaac aacttcattt atccaggaca gcaattgatc gttaaaggtg gaacttcggc
     1861 aaatacaaat acaggttcaa ctacttctgc agcgaaacca aatacaccga atacgtctgc
     1921 tacgacaagt acatcatcca ctggtaatac gatgtatacc gtgaaagcgg gcgagtcagt
     1981 ttggagcgta gcgaacaaac atcatatcac gatggatcag ttaatcgaat ggaataatat
     2041 caaaaataat ttcatctatc caggacagca agtgattgtg aagaaaggtt catctcaatc
     2101 aacaaaccaa caatcagtta ctggttcgaa aacctatact gtgaaatctg gagaatctgt
     2161 ttggagtgtt gctgacagtc atggaattac aatggctcaa ctaatcgaat ggaacaatat
     2221 taagaacaac tttatttatc caggtcaaac gttgattgta gctaaataaa atagctgttc
     2281 gaagaaataa cgattgaatt ccacaaaaat atttgttact ataaggctac ttaaatatga
     2341 caacacttga aaaggaagta gtaaaaagac tgaagtttca gagagcgcat ggctggtgag
     2401 aatgtgcacg gatgcttttg aactcgcctt tgagttgccc ttgctgaata cccgagtaag
     2461 caagcggcgg tgacggccgt tacaacgttc gaggcctttt cactaaggta aaattaggtg
     2521 gtaccgcgta gattacgtcc tataaaaaga gcaatctttt tataggattt ttttatttct
     2581 tttgtgaagt ttgagaaaac aaagcaaaag gaaataaaaa taaacagatt ctgccggttt
     2641 tttcgttata tttataatag aaacaagtgt tttcaataga acaggaggac agcaaatgag
     2701 ttacaatcat aaagaaattg agaaaaaatg gcaaaaatac tgggcaaaaa cgaatacttt
     2761 caacacccat gatgatcctg aaaagccaaa attttacgca ctggatatgt ttccctatcc
     2821 atccggtcaa gggctacatg tagggcatcc tgaaggttat acggcaacgg atatcctttc
     2881 acgtgtgaaa agaagtcaag gatacaacgt gctgcatcca atggggtggg atgcattcgg
     2941 tctgcctgct gaacaatatg cgctagatac aggaaacgat ccagctgagt ttacccaaaa
     3001 aaatatcgaa acattccgac gccaaatcaa ttctttagga tttagttatg actggaatcg
     3061 cgaagtgaat acaactgatc cggagtatta taaatggaca caatggatct ttacgaagct
     3121 ttatgaaaaa ggtctagctt atgaagcaga agtagcggta aactgggtac ctgaattagg
     3181 aacagttatt tcaaatgaag aagtgatcga tgggaaaagt gaacgtggtg gctatgatgt
     3241 agtcagaaaa ccaatgcgcc aatggatgct gaaaatcact gcttatgctg atcgcttatt
     3301 ggatgatcta gaattagttg attggccaga aagcattaaa gaaatgcaaa gaaattggat
     3361 cggtcgttca gtaggagcaa atattgaatt taaagtggct ggtacggata aatcctatac
     3421 cgtatttacg acacgaccag atactctatt cggtgcaact tactcggttc ttgcacctga
     3481 actagatctt gttagagaaa ttacgacccc tgaacaaaaa gctgctgtag aagcttatat
     3541 cgaagagaca gctaaaaaat cagatctaaa gagaacggat cttgcaaaag aaaaaacagg
     3601 agtatttact ggtgcttatg caatcaatcc agttaatggc aaggaaatcc caatctggat
     3661 cgccgattat gtattatctt catacggcac aggagccatc atggctgttc cagcacatga
     3721 tgaacgtgac tttgaattcg ctcaaacttt cggactagaa attcttccag ttatcgaagg
     3781 tggagatgtt gaaaaagctg cttacacaga agatggtacg cacatcaact ctgaatttct
     3841 agacggcatg aacaaacaag aagcaatcga taaaatgaat gaatggctag aagaaaatgg
     3901 cgtagggaaa aaagaagtca gctatcgctt acgtgactgg ctattctccc gtcaacgtta
     3961 ctggggtgaa ccaatcccaa tcatccattg ggaggacgga acagtcacag ctgtcccaga
     4021 agaagagcta ccattacggt tgcctaagac aaaaaacatc aagccaagcg gtactggaga
     4081 atctccatta gcaaatatcg aggagtgggt caatgtcgtt gatcctgtaa caggtaaaaa
     4141 aggccgtcgc gaaacgaata caatgccaca atgggcagga agctcatggt attacctgcg
     4201 ttatatcgac cctcataaca aaaaagaact agcagattat gaaaagttga agcgttggct
     4261 accagttgac atctacattg gtggagcaga acatgcggta cttcatttac tatatgcacg
     4321 attctggcat aaattcttat acgatatcgg tgttgttcct acaaaagaac cattccaaaa
     4381 actatacaat caaggaatga tcttaggcga aaacaacgaa aaaatgtcaa aatctcgtgg
     4441 taatgtagtc aatcctgatg atgtggtcga aacttacggt gcagatacac tgcgcatgta
     4501 cgaaatgttc atggggccat tagatgcttc gattgcttgg agcgaaaatg gcttagaagg
     4561 aagccgtaaa ttcttggatc gtgtatggcg tttgatcgtt gatgagaaca ataaaatgcg
     4621 tgaccgtatc actacattga atgatggcaa gcttgataaa gtgtatcatc aaacagtgaa
     4681 aaaagtgaca gaagactatg aaaatcttca tttcaataca gcaatctccc aattgatggt
     4741 ctttatcaat gaagcctata aagtggacgc tctgccttat gaatatatcg aaggatttgt
     4801 tcaactactc gcaccgattg ctcctcacat gggtgaagaa ctatgggcaa tccttggaaa
     4861 tgatggtggt atttcttacg caccatggcc aacatacgat gaagcagcac ttgttgaaga
     4921 cgaagtagaa gttgttttcc aagtaaacgg taaagttcgt gcgaaaagca atgttccccg
     4981 agacttagga aaagatgagc tagaaaaagc agcattagcc aatgaaatag tgcaagaata
     5041 tattgaaggc aaaacagtca gaaaagttat tgcggtacca aacaagttag tcaatatcgt
     5101 tgctaattaa ttggctcttt gtcaataagg actgatgagt tgtgcaaaat caaatctggg
     5161 ccagaatgaa cttcattctg tctcagattt tttgttattt caaattgatt aattgattga
     5221 tcaaaaaggt tttgattttc atgttctcaa acacgttcaa tgtctttttc ttctgaatgg
     5281 tcttgtaaga gatctctgta atcatcaaat ttttgtctat taatcttagt attttttgat
     5341 gtataaacta cctgaaattg agagtacata ccaaattcat aaatttttta ttagtgggac
     5401 tagtgattgt atggattttg acgcataatg aatgaagtat gagaaaagga gagacaaatg
     5461 gatcaagaac tatttattat cgaagtatta tcagctaaac aaaaaattat cgagaaagcg
     5521 aaaaaaagag cgaaaagaat aatgttcaag cttttaagct tagtattttt ctctggtttg
     5581 ctttttttcg gagctgtatc gttcctatat cgccatatga gtgcagtagt ccgttcagct
     5641 gaagggatgc aaaaattaaa taatttgtat cacttttcgt ggggagttgg cggaataaca
     5701 attgttgtgc ttattgtttt tcttctactt atttggctcc gttaccatca attgtcgcgt
     5761 catttcgatc aatggcttag tcaaagagca caacagtttt tacataataa accaacttct
     5821 caagatgatg actatcttta ttttgagaag acttcgacga gtcaagcttt tcggatacaa
     5881 aaaagtgatt gccagttatt gctgagatca cttgatgatt tacctatcta cttaggcgaa
     5941 agcaaatcaa aattcggaat gaacacattc catatttttt tactagaaaa cgaacccaga
     6001 tgggaaaaaa gccagtcttc gcctggtgga tacctgacaa ctaagatgag tacattagtc
     6061 actggttttt tcctcattct tttagctgga acaatctatt atgggagata cactcagcca
     6121 aaaatgtctt ctcatataaa tgctgcttct cacatgacaa tgaactcaac taacgatacg
     6181 catatacaaa agacaaatga agggttgtta gctgatccta agcaaaaggt aaatttgaca
     6241 acacaaaaga taaatgatct caaacttgat acagagacgc actctctcta tatgacaaca
     6301 gatagtggtg ctaattgggt atttgtgccg attgattcgc aatggttgcg ttttggcgaa
     6361 tacaccctga caacggggct gattcctgaa ggttattgga tggacaacac atatgatatt
     6421 tccgccgatt tctcatggtt tatctattct tctggggatg atgtacgtct gctgacttca
     6481 agagataatg gtaaaacatg gcaaacgtct attatcaccc aatcagttca gccgatacgt
     6541 tatcgaaaag cggtatttta tggcaacgat ggtctattta tctgtacatc agccagtggg
     6601 atgtctgcag aaactttgta catttacact actatggatg gcggaatcac ttggataaaa
     6661 agtggaagta caatgatcaa tcagccagta caaaatgtca gcttcgttac aacttcttta
     6721 ggttttgttt cgacacgtga aagtttgttt tatacaaaca acggaggacg ctcattcaaa
     6781 gaatcagttc tgtcgattcc ggaaaactat cagacagggg ggatagatct ttttcaatca
     6841 ccaaatgaag ttactcaaat aagcgcgaat acgttagaga caaaatttta tttgttgaaa
     6901 aaaggaccaa tcgatgcagg gaaaatgttt gcctgcctct tccgttcaac tgataacggg
     6961 gaaacatggc aattcgttca gcaactatcg caagtagaac aaggagatta aagaagagag
     7021 attgtgagcc tgacgttcag caccgagtca caagaaacaa attcaaaaaa cagcttctca
     7081 tatttgttga atagtgtaac tcgtgattcg gagttgacaa gagcaacaag aagcgatttt
     7141 gaagtatagc ttctcgtatt ttacaagatc gtgtcttatt gccgataggt tgtctcttga
     7201 acaccgttta ttcgtaaaaa gggattgtga caagtctttt gtcactactt accgaacgaa
     7261 actgaaggag ctatccaaaa agtatcatta gtattattct ttgtgtttct agtggcacca
     7321 ctagtacatt taaaaaagct atcgatcatg gactaatcat acatgatcga tagcttttta
     7381 gataatgttt gttacgctat ctgaaattga ctattataaa gactggcata aagcgttggt
     7441 gattggagta gttcagaatg tgttcctttt tcgacgatat cgccattctt cattacaagg
     7501 atcatgtctg cattttcaat cgttgacaaa cggtgtgcaa tcacaaaact tgtcctatct
     7561 tttgtcacag cttccattgc ttgctgaatc aatgcttctg ttcgtgtatc tacgctagaa
     7621 gttgcttcat ctaaaataac gacaggagga ttcgctagaa tgactcgagc aattgtcaat
     7681 aattgttgct gtccttgaga taacgtgccg ttttcgctag aaatgatcgt atcatatccg
     7741 tctggcagtg ttcgaataaa gtgatcacat tgtgcgattt cagccgcttc gataatttgt
     7801 tctcttgtgg catccatttt cccgtaggcg atattatcag ctaccgttcc ttcaaacagc
     7861 caggtatttt gtaaaaccat cccaaaagaa cgacgtagtt ctccacgtga aagcttggta
     7921 atatcaagac cgtcgaattc gatctttcct ttattcagtt cataaaaacg catcagcaga
     7981 ttgactaaag tcgtttttcc tgcaccagtt ggaccaacga tcgctaccgt ttgttttggc
     8041 tttactgaaa aatcgacttg attcatcaat attttatctg atgtgtaacc aaactgtaca
     8101 tttttgaaag ctatttttcc tttcggttgt tctaaaagtc ttgcatctgg aaggtctgga
     8161 atttcatctg tttcatctaa tatctcaaat atcctatcga ttgctgccat tgcggattgg
     8221 atcgagttga tcacataaga agcggtagaa attggttcag aaatctgatt gatgtactga
     8281 agataagctt gtaagaagcc gatcgtcaca ccacctgata gaacaagcat agcaccgaat
     8341 accgcactta tgatgaaggc gagttgatta atgaaacgaa tggccggata gatagcacag
     8401 ttcataaatt gagcacgttt aaaagcgttg tattgctttt tatttacttc gtgtatagca
     8461 gcttctgcat gagcttgctg attgaatgtt ttgaccacta gattaccagc taagatttct
     8521 tccattcgat tatttaaaac acccagttca tactgattct gttcagaaat ctgtttgttt
     8581 ttgttcgcaa tcttagttgt aacaattgaa ctgccaacga tcagcaataa gacgagcaaa
     8641 gtaagcttgc tttcgatata aaacagcatc agaccagcag cagctattgt tacgacagaa
     8701 gtaaaaaatt gattgatacc tgtcaataac acttgagaaa gttgattgag accggtagtt
     8761 gtccgactaa tgatgtcacc tacttgatga ttgtcaaaaa acgacatcgg taatgttttg
     8821 aatttttttg tgatttcttt tcgcaccctc aatgtgatct tttcgcttaa cgctgccatt
     8881 gtccgttcct gaataaatga agtgatgctg cttaagactg aacagccgat cagcaataag
     8941 acagggaaca acaatgcttg tttgatatca ctgaaagaaa gctgtccaat acctgttttt
     9001 cttatttctg ataagagatc atcgatcccg attcccatga tcaagggcat ggctacgact
     9061 aataggttcc cgaataaact gcatagaaac aatccgtaaa aatatttatg ttcaggtttg
     9121 atcattttcc aaaaacgtat caaagcacgc gtgttcaacg tttttttctt ttccatcgta
     9181 tcccttcttt ctatattcct tgtgattttg caaactcctg ataagtttgg caattagaca
     9241 gtagttcttc atgagtacct tgtccaacga tttttccttc atctaaaaca atgatgttat
     9301 ctgcttgttg gattgtactg aggcgttgag caacaatcac gagagttttc tccgacatgt
     9361 gtttatgaag ggctgctctt aatgctgcat ctgttttata gtctaaagcg gagaaactat
     9421 catcgaatac atagatctct gctggtttga tcaacgctcg agcaatacat atccgctgtt
     9481 tctggccccc tgaataatta gaaccgcctt gagcaacaaa gctgtccaat ccgtcaggaa
     9541 gggtgtccac tagttcttct aattgagcga tcttgatcgc tttagacaac tcttctgttg
     9601 aggcatattc atttcccatc tgcaagttgc ttaaaattgt cccgctgaat aagaaagcct
     9661 tctgagggac ataactgatg tgggagcgga tctgttcttg agtcatttga gtaagaggaa
     9721 taccaccaag ggttactttt cctatcgtag ggtcactaag tctcaagaga agtttcgcca
     9781 aactactttt tccagatccg gtcccgccaa cgatcgctgt tgtttttcct ttaggtattg
     9841 tgaaatcaat atcttctaga actggatcag agatttcagt atatttaaat gtgacatgtt
     9901 caaaagcagc tgcatttgtc tgatcaaagg ttggttcagc tactgttttg atcgtctctt
     9961 cctcaatttc gatctctgct tgcagtattt cttctatccg tcgtaatgag gcaaacgatt
    10021 tagggataat gaccaatacc atagctgcga tcatcaagta agcaagtgtc agcatagagt
    10081 attcgactac agcagtcacg acaccgattt gtaatgctcc ttctgctaca agatttccgc
    10141 caaaccacaa aatagaagca taaacgattc ccatgagcaa aaaagcaatt ggagtgaccc
    10201 aagcaaatat tcggttcact ttgatcattt gattagcata gtcagcaaag ctcttattcg
    10261 ttcgcttttc ttcatatgat tgattatcaa aggcacggat catattaatc ccagtaaaga
    10321 attctctcaa tgtgatggtg atccgatcca tttttggctg gatactcaaa gataaaggtg
    10381 cccctcgttt catcagtcga taaacaatcc atacataaaa aacaacagag atcaatgtaa
    10441 cccatgttag taatagagaa taacgaaaca tcatgagaat ggtgaaaacg caaataaccg
    10501 gtgctggtaa gatcatttgg caaaataaaa caatcatttg ctggacatta tcgacatcat
    10561 tcgtcattct tgtcaaaaga gatccagaac taaaacgact gatatccttc attgacagct
    10621 gttggaattt acggaaatat ttctcccgcg tttggaatcc aaacctagcg gaagccaatg
    10681 ctgaaaagta actagaggca actgcagcga ttgttcctag aaacgctaca agcagcattt
    10741 caactccaat tttttttata actgaggctt cgccacttgc tatacctgtg tcgatcagct
    10801 ttgctactaa ttttggtaca cctaatgtac cggcgatttc taaacagatc atcacaaagg
    10861 taaggagagc gagtcctttg tttttctgta agaatgtttt taagatagac atattattcc
    10921 tccttttttc ctgatacttt tcgattctat accttcaagt aacttgaagg tcaatagaaa
    10981 aaagaataaa ctgaaaaaga ggtgagaaaa aatgaaaaaa atgtatacga ttggccaagt
    11041 atcggaacta tttgatttgc caaaatcaac gatccgctat tgggatgaac aaggattaat
    11101 ccgttcaagt agacaagaag agaatgatta tcgattgttt gatatcgatg atatttttat
    11161 gctttatgat atcgtttttt atagaaaatt agatataccg atcaaacaga tgaagaattt
    11221 atatggaaaa acattgacgg agttatatga gacacttgat gaaacagaaa gaagaatcca
    11281 taaagaactg gtggtgatga aacaaaaaca aaaagagata agggagcaaa aaaaacagtt
    11341 gaaattgatg atcgatacaa acgaggaaga atttcctgta gaagagatcc cttttgactg
    11401 tatgatctca acggagtttg aagatattgt ggaaatcaaa aaatttctac ctaactattc
    11461 aagctttggg atgatgagta tgccagcttc cagttcacag acgatgtatg gattttttat
    11521 cgaccctagt gaagtacatt tatttactca agacgtgatt tgggagaaaa aagatacggc
    11581 tgtttatcgt cgattcttat tgaaatccga gatgaaccat gcggaacgta ataatatttt
    11641 agaaatcaga gaaagaatgt atgaaaaagg atggaaaaca ggagaagtga tcggccagta
    11701 tctattgacg aatacggatg aaaacaatat cagaactgaa tattatcatg catggataga
    11761 aatgaaaaaa taggaaatat gtgtttaaga taactcgaaa atgatggttg ttttagttat
    11821 aaagaaaaat tttctggttc gattacgggt ttaatgtggg ggattattag tcgtggatag
    11881 atagaatttt agttttttag aggaaatatg tctttaaaaa tggaatggtt gcaattctta
    11941 gcgatatata cgatgtaatt gtcctcttaa aaaagtatgg aaaaataatt gtattgggtg
    12001 ttatgtataa ttcatggtga actgagacta atgagaagaa aaattcatat tgttatgagt
    12061 gagattgtat cagatgagaa tgataaatta aataggtgca taaaagcatt acacttaaaa
    12121 tacattgttg cttacattat tacaatatgt taataaggca aaaataaata gatggattga
    12181 ataaacatat tttttaatca aatagtccct actgtgactt tcctcagcta tcggtgatac
    12241 tacc
//
LOCUS       DANKKG010000081        12043 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000081 DANKKG010000000
VERSION     DANKKG010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12043
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.081"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(1..347)
                     /locus_tag="PW004_001841"
                     /pseudo
     CDS             complement(1..347)
                     /locus_tag="PW004_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
     gene            complement(353..1216)
                     /locus_tag="PW004_001842"
     CDS             complement(353..1216)
                     /locus_tag="PW004_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2726808.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(1230..2126)
                     /locus_tag="PW004_001843"
     CDS             complement(1230..2126)
                     /locus_tag="PW004_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2726809.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(2301..3590)
                     /locus_tag="PW004_001844"
     CDS             complement(2301..3590)
                     /locus_tag="PW004_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2726810.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(3562..5079)
                     /locus_tag="PW004_001845"
     CDS             complement(3562..5079)
                     /locus_tag="PW004_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HDL2726811.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            5277..6167
                     /locus_tag="PW004_001846"
     CDS             5277..6167
                     /locus_tag="PW004_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HDL2726812.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(6240..7064)
                     /gene="nadE"
                     /locus_tag="PW004_001847"
     CDS             complement(6240..7064)
                     /gene="nadE"
                     /locus_tag="PW004_001847"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HDL2726813.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(7076..8548)
                     /locus_tag="PW004_001848"
     CDS             complement(7076..8548)
                     /locus_tag="PW004_001848"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HDL2726814.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(9185..9547)
                     /locus_tag="PW004_001849"
     CDS             complement(9185..9547)
                     /locus_tag="PW004_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HDL2726815.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            9770..10522
                     /locus_tag="PW004_001850"
     CDS             9770..10522
                     /locus_tag="PW004_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL2726816.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(10662..11585)
                     /locus_tag="PW004_001851"
     CDS             complement(10662..11585)
                     /locus_tag="PW004_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2726817.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(11600..11872)
                     /locus_tag="PW004_001852"
     CDS             complement(11600..11872)
                     /locus_tag="PW004_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726818.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT         3788 a         2429 c         1969 g         3857 t
ORIGIN      
        1 tccaggataa aagaaacatg gaataaataa gtaaaactac atatccacta attgaaagta
       61 cgattgtctg ttcttctaat aattgattaa caagaaaata aataatgatc gttaacagac
      121 taaggaacaa gttcttacca aaatgttgct ttattccttg aaagaaaaaa taaatgatat
      181 ttcctgatcc ctcacctttc cattgcgcca ttacataaaa aaacagcaca tgtagcaggc
      241 acgatagtaa taacaggcaa acacacgatt acccagagaa tacctaataa gatgtaatca
      301 gaaaccacaa tcaactgtcc ataaagttta gtctcaacta ttttcatctt cctcactctt
      361 tcattccaga catagctgtt ccttgaacaa aatatttctg tgcaaaaata taaaccacta
      421 gtaatggcag cattgaaata accgttccag ccattacagg tccataaaaa gaaatgttct
      481 gccctgtaaa taaagctaac cctgctggta aagttctcat ttttgaacta cttgtcaata
      541 tcaatggata aagcaagtca ttccaactat tcatcagcgt gaaaatccct aatgataaaa
      601 gagtaggttt gctcaatggc aacatgattt tcaaaaaaat attaaactca cttaatccat
      661 caatgcgtgc tgcctcttcc aaagaatctg gtaaagtaat aaaaaaggaa cgcatcatga
      721 agataccaaa tgcagttgtc atacgaggaa tgatcaaacc tgcataagta tcaagcatgc
      781 caagcaaatt gacttcaata aatagaggaa tcatgaagac ttggaatggg atcatcattg
      841 tcagcaaaac gaaatagaac aaaactgatt ttcctttgaa acgcatacga gcaaatgcat
      901 accccgcaag agaatcaaag aatacagaag tgatcactgt ccctccagca aaaatcactg
      961 tatttttgat gtaatctaat aaagggatcg ttgtccaaac cttaatatag ttatctaggg
     1021 tatagctatc tgcaaatatt ctaggcggaa atgagataat gtctttttcg tgtttaaaag
     1081 aagaaataat cagccacaaa aaaggaaacg caataaccaa caatataact agcatactaa
     1141 tcgttcgaat gattttttta ccactaactg cttttcgact attcacagta actgtaatcg
     1201 agctttctgg tgccatacag gtttccctcc tatttcattt gtttttcttt ccgtgacatg
     1261 tacacattag tggagatagc tatagttgca acaatgataa acaaaaccga agacagtgct
     1321 gatgcatagc ccaattcata aggtgctgta aatcctcgac tgtaaatata ttgaacagct
     1381 gtttctgtct taaattgtgg gccaccagcg gttgcaacat atacttggtc aaacacttgc
     1441 atcgaaccaa tcagattcgt taaaatacaa aaacctaagg agggaacgat ctgaggtagc
     1501 gtaatgttaa aaaactgctg tcgatttgtt gcaccatcca tttgagcaga ttcatacaaa
     1561 gagggagaga ttccctgaat agcagtcaag agaatcgtca tagtaacacc aaaattcttc
     1621 caaacagtca tgatggctac cgtcggcata gcaagggttg gatcgcgaaa aaattgaggg
     1681 atcggcaagc cgacttgtga aaacaagtaa ggaatcgccc ctacattcgg atcaagcaac
     1741 atagaaaaaa taatcgcaat tgacgtcaat gaacaaataa ctggaatgta aaatgtcgaa
     1801 cgacaaaaac gattgaatct ggtattttta gataatgcta ctgctgctaa taaaccaacg
     1861 ataatttgtg ttggcgtttc gagtacggtg aaatataaag aatgaatcaa cgaattaact
     1921 gctcgttcat cactaaatat acgtgtgaaa ttttcaaaac ttacaaaatt tcttgatgta
     1981 aagaaaatat tcaaatctgt aaaactaata gcaaatgttc ctaaaagcgg tatgacagta
     2041 aataataata aaactaaaaa agatggagtt aaaaacaaat acgcagcacg atcaggacga
     2101 ttccaatatt tggtgaatcc gttcatatag tccacctcat ctttcatttt gataaaatat
     2161 acaatcttgg tttaaacgaa tctgaataag atatccgaac aatcgctgtg atttttgcaa
     2221 taacacctat ctgcaaatac taggtcgatc attcagatat ctgtatcttg ccaacagtct
     2281 atcctgtata aagttctgta ctatttttgc agtaattgat tgatggcatc atctgcatcg
     2341 ttcatgagtg tctcaggatc ttctcctgct aataattttt caatcagtgg attgatgata
     2401 tcgttattga tcgcagagat ttgggtaaaa cctggtaaat atggctctgc atagtccatt
     2461 tgtttcgata attcagatac aataggatca ttttttactt cttcatcttt tgctactgaa
     2521 tccaagtagg caggaaaacc attttcgata ctccattttt taccaatttc agttgtattc
     2581 caatatttca aaaactcata gacagcttct tttttattat tatctgtact tgccggaatg
     2641 gcatatccta cgccatctaa aatagctgtt tttttgtctg aatcactccc ttgtggtaag
     2701 gtagtcacat tataatcaat ctcgtttgct ttcaatccgt tatttaacca tggcccattg
     2761 atttcaatgg ctagttgacc agcattcatt aaattgtcca tttcggcgcc agaaatcgat
     2821 tctggaccag tcttgtctac atggatcatt ttttggatcg cttttaacgt tttaaggttc
     2881 tcaggagacg caaattcaga cttagtataa tcatcgttca ctaatttacc gccatttgct
     2941 aacatccagt tatataacgg tgcagttcca tccttattga agacaaaacc tgatacattt
     3001 ttactactat cagttaattt tggtgccatt tctgtcaatt cttcccaagt agtaggtggt
     3061 tgttcagtat ctaatccagc ttgtttaaac aaatcattat tccaatacat atacatgcct
     3121 tgtacctgca tggggacaaa atactgttta ccttctactt cacctaattc aatagccgtt
     3181 tctttaaaat cagatttatc tactccatca tattcccaga aatcgtctaa tggttgtacc
     3241 gctccgtttt ctacatactg tgcaaaatct ggataattta atgcaataaa gtcaggtgct
     3301 tttttagaag aaatcgctgt aggaagtttt tcattcaaat tggcccaagt catgatatcc
     3361 atttcaattt tgatgttttt atcatttgtt ttattaaatt cattaacgat ttcttgcaat
     3421 atttcaccat cagatgcagt aaacccattc caaaaagtca aagtcacttc tttatcagaa
     3481 tcagattctg ctgaacttcc gccaccgcaa gctcctaaaa tacctgttgc agctaccaac
     3541 aaagagccaa ttaaccattt tttacctttc attttttgtt cctccttcat ctgataaacg
     3601 acatctgacc acattccaag atagcggttt caataaacaa ttgattttcc catcttcagc
     3661 ttttacattt gttgaatgac ttggttttac ttgttcactt ttaggaagat tagttgcttt
     3721 tgtatcaaat ccactcattt cagaaaaatc aatcactccc tctaatgata attcagacaa
     3781 gtgaacagtt agattcattt cttcttctcc acggttaact gcaaatatga caagttcttt
     3841 gtcatttatt acggctatac tttctaaata cggcacttct tcaaaatctt ccgtcgcata
     3901 gtttgctgaa cgaatttgtg ggcttaacac ctctccttgt ccatacacag cagcttgcat
     3961 aaatggataa tagatcgtct gtctccaagc ttctccgttt gtttctgtcc tgattggtgc
     4021 aataacattt accaattgag caagacaagc aatctttact ctatctgcat gtttcaataa
     4081 agtgattaat agacagccta atagtaatgc atcttcaaaa ttatagttat cttctagtaa
     4141 aggaggagca atctgccaag gcgctgcttg ctcatcttgt tccgctgaat gataccaaat
     4201 attccattca tcaaaggaca ggttgatctg cttcttacta tgtttcttcg ccttgactgt
     4261 atcgcacatc gctgtcactt cagaaatgaa ccgatccatg tctaaggaac gtgcaagata
     4321 atgagaaaga tcattttttt ggttgccata atattgatgc agagacaaat aatctatttg
     4381 atcgtacgct tgatcaagaa ctgttaactc ccaatcacca aatgtaggca tttgccgatt
     4441 cgaacttccg cataaaacca cctcaattga atcatctacg agtttcatcg ccttagccgt
     4501 ttcctctgct aaccgtccat attcttccgc tgttttatgt ccaatctgcc aaggaccgtc
     4561 catttcattc cctaaacacc atgtcttaat tccaaaaggg ctctcttgac cattcgaacg
     4621 ccttaaatca ctccaataag ttcctttagg aaaattacaa tattcgacta aattacgagc
     4681 tgcatctact ccacgagtac ccagattgac agccatattg atttcggccc ccacatgctg
     4741 acaccagtta taaaattcat gaatcccaac ttcattcgtt tcaattgtcc gccaagctaa
     4801 atccaacctt ctaggtctat ttgctatggg cccaatactg tcttcccaac gaaatcctga
     4861 cacaaaattt ccaccaggat atctaatcat gggaatgttc aattctttta ccaagtgaat
     4921 gacgtcttgg cgaaatccac ttttattagc tgatggatga tctggctgat aaatcccttc
     4981 atatactgct cgtcccatat gttcaataaa tgaactgtac aaccgcggat caactttgcc
     5041 tacacgcttg acactactaa tcaacattgt ggcttccata actgacctcc tttaatttat
     5101 acatgaataa tacacctcgt tgaaagcgtt gtcaataata taaatgttaa acttacttat
     5161 tttttatcga ttttaattaa ccaaaatatt aataatgatt aaaacaaata atttaatata
     5221 acttttatta acaaatatgt taatatataa gaaaaacctt ttaagaggtg atatatatgc
     5281 aactacaaat aaacgaagaa tccttaccgg tatatgaagc attagctagt aaaacaagga
     5341 tcaagatcat ccagctactt tccaaaaaaa agatgaatgt caaagatttg gcaaaagaac
     5401 ttggagtgag cagtgctatc acgacgatgc acgtgaaaaa gctagaggaa gcaaacatca
     5461 tcaaaacaga aaaagtagga caacaaaaaa tttcgagcct tcgtgtcgat aaaatcgata
     5521 tcagttttcc agaaaaaata ttcaatgctt ttgatacgaa agaaacatcg atacctattg
     5581 gtcactatac aaattatgca attgaaccaa catgcggttt agctacgatc catgatttta
     5641 tcgggaaagt ggatgaacca cgctatttta tggatcctcg aaggatggat gcccgcattc
     5701 tctggtttac cagcggattt gtcgagtatc aggcacctaa ttttttaaat acagaggata
     5761 cattggagat gttggaagtt tctgtggaaa tttcttctga atttcctttt tcaaatgaca
     5821 attggccttc agatatcact ttcagcctaa atggtgtgga gctaggaaca tggacgagcc
     5881 ctggagattt tgctgatatc cgcggtaaat atacgcctga ttggtatcca gataatctta
     5941 atcaatacgg cttgctgaag acgatccgaa ttaccaaaca cttgacgaat atgaatggag
     6001 aacccttgtc aaacatcacg atcaacgaca tccctaaaga gcaagacact tggcatttgc
     6061 gaatcgaagt gaaagacgat gccaaacatg tggggggctg tactttattt ggtaaaggat
     6121 tcggtaatta cgatcaggat atcaaattaa aagtttacta cagctgatat ttttcttttc
     6181 tattttataa aacaaaaaca gccaatccct atgaagggac ggctgttttt gctttattgt
     6241 tatttccaga aatcatccaa aacggaaatt ggcaaatgtc gtttatgttg tgtcttgttc
     6301 caccatgctt cgattttttc ttgtgcttga gcgggaactt cttttccttc tagataatca
     6361 tcaatttcat tatatgtgac acctagtgcg acttcatctg caatcattgg tttcccatct
     6421 tcaagatctg cagtcggaat ttttgtgtat aatttttccg gtgcattcaa tgcttgaagc
     6481 aattgtttcc cttgtcgttt gtccaaacgg aataatggca aaatgtcggc tccaccatca
     6541 ccaaatttcg tgaagaatcc agtaatgttt tctgcagcat gatctgtccc taatacggct
     6601 cctgctcttt ctccagctac ggcatattgc gtaatcatac gttgacgtgc tttgatattt
     6661 cctttgttaa aatcgctgat tcccacacct gcttcgctta atacttgtac ttgtgcatca
     6721 acagctggtt tgatatttac tcgcagacta acatcgggct ggataaagtc aagagcagct
     6781 tttgcatctt cttcatctgc ttgttcacca taaggaagac gaacagcgat aaattgatag
     6841 ttttcatctt ttgtttcttc acgcatttct tccatcgtta gttgcgccaa acggcctgct
     6901 aaagtagaat cttggccgcc gctgatacct agtacaaaag ttttaagaaa cggatatttt
     6961 ttcaaatatg ctttcataaa gtcaatactt ttacgtattt cttcttttgg atcaattgtt
     7021 ggtttgacac ctaattcatt gataatttct gtctgcaggc tcatttattc tcctcctatg
     7081 cttctgtatg aagttctgct acgctttttt tcattcgttc catgatagcc attttatggt
     7141 tccagcattc agtagacaga tcgactgggt atttttgcgg attcaagtct cgtttatact
     7201 cttcccatag tgaatctaga ttttcttttg tatattcttt gatttcgttt agtgcaggta
     7261 attcgtaaac tctttttcct tcgataaaga tatcttgtaa aactgggcga gctgtaaagt
     7321 ctcgaacagt tttattgata aacgtatgca ctggatggaa catgaagata gcttcttctt
     7381 ctcgagggtc ttcatcccat aatgtcacgt agtctccttc agatttccca tcaaaatttc
     7441 gagtgatgcg ccacacttgt tttttccctg gagtagttac tttttctgca ttgcttgata
     7501 acttgatcgt atccatcatt tttccttcat catcttcgat cgatactagc ttgaatactg
     7561 caccaagggc tggctgatca taggctgtga tcaattttgt cccaacaccc catacatcaa
     7621 ttttagcttt ttgcatcttt aaattcaaaa tcgttgcttc atccaagtca ttcgaagcat
     7681 agatttttgc ttccgtaaat ccggctgcat ctaattgttc ccgaacacgc ttagatatat
     7741 aggccatatc tccactgtcg atgcgtacgc cttggaagtt gattttgtca cctaattctt
     7801 tcgccacgcg aatcgcactt ggcacacctg atttcaacgt atcataagtg tcgactaaga
     7861 acacgcagtc tttatgggtt ttagcgtagg ccataaatgc ttcataatca tttccataag
     7921 attggactaa cgaatgagca tgggtcccgc tagctggtat cccaaatatc ttcccagcac
     7981 gtacattgct tgttgcatct gctccgccga tatatgccgc tctagttccc cagactgctg
     8041 catccagttc ttgtgcccgt ctagttccaa attccaatag tggatcttct ccgatgacag
     8101 atttgatacg agctgctttt gtcgcgatca acgtttggaa attgaccata ttcaacagtg
     8161 ctgtttctac taattggcag tgcgctaaag gcccttccac ttggattaat ggttcatttg
     8221 caaaaactag ttctccttca cgagcagaac gaactgttgc tttaaattca aaattttgta
     8281 aatactccaa aaaaccttca ggatagactt ctaatccacg caagtaagca atatcggaat
     8341 cattgaacgt cagattctct aaataattga ctaaccgttc caatcctgca aaaaccgcgt
     8401 atccgtggtt aaaaggcatt tctcgaaagt aacattcaaa aacagcatgt aaatcagctc
     8461 gacctaattc ccagtatgtc tgcatcatat tgatttgata tagatccgta tgcagcgtca
     8521 aactgtcgtc tgggtaagta ttattcatct tgtattactc ctcttggcaa gttttcattt
     8581 cctacccatt ataacaaaaa aaattaatag gaaaagaaat cttccatttt ttctgaaaaa
     8641 gcacatgttt tacggatatt tcttgctaaa aatgaactcc atctgctaaa actatccttt
     8701 cttattggta tttaacctaa acgagaaaga atacttattt tattcaatgt atccgagaca
     8761 cgaagaaatt agcgttttct atttttctta cttgataaaa taagtagatt ttcctactac
     8821 aatcgtagcc gttaagatac cgatgattga acaaatataa ccaaatgtct ctacaacata
     8881 atcaatggca aaagtttttg aagcaataat acgtctttcc ttttttgctt cttatcccca
     8941 aagaaacgga tataacttat tatgtattga atttttttgg aaaggcgaat tatgacaaga
     9001 tataccttgc gttgaaaaaa ctcgcggaaa aaatatcaca cgtttttaaa aagatacaaa
     9061 gtaagaaagc agctgtcaat agaatggaaa caaaaatggc gagacatcaa tcagtctcgc
     9121 cttgtttttt attgtataca ttagatatgg agcaggttac cataaagatg attattagtc
     9181 gttattaaat agaatccttc gtattttttt gttctatcac tacaatatct ttatttggac
     9241 gtttgaattg tattccggca cgttctagct cctgtttcac tcctacatgg actttgacat
     9301 cttctcgtgt catttttaaa acgtgatgaa tcgcatagtc gatttcttgc cttgtgattt
     9361 ttttcttcgt attgatcatc acgacttgct ttttatcata tgtgtctacc aatagcagtt
     9421 gtgttcgatc taacgaaaat aatttgtaat aattaatagc cgtattccca aaaaagaatt
     9481 ccactacttt aggataaaat gtcttaatat ttaaattact attcacaggt gtttcgatta
     9541 gcttcatctc attcctcttt tctatatagt cgatttagat ccaatcttaa gttatgttac
     9601 catctttcct agcattaagg aatcggttac aattgaacct ttaaagacaa aaggatttac
     9661 cttttatcac ttaatagtat aacataattt cgtgagaaaa aaatacaatt ttttcttgac
     9721 catctcgtta cgtaaaggat tatgcttaca tttgtcagga ggaaaaataa tggagtacac
     9781 aataaagaaa atgtcagaga tatcaggagt cagtccgaga accttgcgct tttatgatga
     9841 gattggactg ctaaaaccag ctaggatcaa ttcttcaggt tatcggattt atggcaaaaa
     9901 agaagtcgat cgtctgcaac acatcttatt ttatcgaaca atggcattca agttggatga
     9961 tatccaagag gtcttagaca atccatcttt tgaccaccag aaagctttaa tcaagcatca
    10021 gcaaatgttg ctggaaaaac gggcacaaat cgataccctc ttgacaactg tccaacagac
    10081 acttgatatg tacgaaggag gaagaaaaat gagtgacaaa gaaaaatttg aagggtttaa
    10141 acaacaaaag ttgaaagaaa atgaagaatc ctatggacaa gagattcgac aaaaatacgg
    10201 aaaaaaaaag gttacagcat ccaatgaaaa atggatgaat atggataaag aaacgtatga
    10261 agaaatgcaa actgttgaaa agcagatgtt atttgatctc actctttact taaaagaacc
    10321 agaagatgaa agcttagcag accgtatctt ccaagctcat aagaagtggt tctcttacag
    10381 ctggcctgaa tatcagccaa aagcgcataa aggactagga atgatgtacg aagccgatga
    10441 acggttcact gcatattatg acgagcgaag tggtcatggt gcagcaaaag ctttaaatga
    10501 aattatccaa aaaaaggcgt aatcttttac atgatccaac aaaaaagtac acgtaaaatg
    10561 acctaataac aaaacagcag acacccaagt tagattttta tgctaacttt tggtgtctgc
    10621 tccattcttt cacaagtttt ttcatcctac tttaaatata ttcatcgttg aataaacgta
    10681 taaggtattt tcactttatc ctttttttct gtaagaaaca attgaaatgc catttctccg
    10741 caagcttcca gatggtggtc gatggttggg aaaccaagaa gctcgctgat cagcaaatta
    10801 tcgcgtccaa tcaccactgg tgtcttatct tgcagatagc tttgcaagat gcctgctgca
    10861 accatatccg aatttgtaaa gacaccatca actccaagct ctttaaaaaa gaagcctgct
    10921 tgttttcctt tctctgcatc tatacaatcc caaaaaacaa gtgattcatc aaaagatgga
    10981 aaaagttgat gacatagctg caaagtgatt tttgagttat aacttagcct gccacttcgc
    11041 cccaacgtga tgcccaattg ctttatacct tgctgcttca aataagtaag accttctgta
    11101 attgattgtt ctcgatcaat aaagacacag cttgcctgtt ttcccttgat ttcttcacaa
    11161 aaaactagag gtccatattt ttgatatgcc aataaatcat ctagtgtatt ggcacgagac
    11221 gtaatgatca atccatcgaa agctttagct gcaaattctt caagatacct tcgctctttt
    11281 tctttatgat agcccgtcgg taaaagtgtc actttgtaat cataatcaaa cgctgcttca
    11341 atgatcccac tgatcaactg atcaaaatac ggatgattgg tataaggcag aatcactcca
    11401 atgcttttcg tttttccata acttaactgt ctggcatttg cattagggat ataatcgact
    11461 tctttcatga ccgcaagtat ttctttcctt ttttcctcat ctacatatgg atgtccattg
    11521 attacacgtg aaatagtaga aactgaataa cctgtgagct ttgcgatatc tcgaatgttt
    11581 gccactgctg cttcgccacc tatacaaaaa gattttcttg taaatagtct accacagctg
    11641 gaccgcattc aaaaaactga tacatttttc cttcatgcga aaaactgatc cgctgcccgc
    11701 cccaatatat atcttttgtc aaattttcga tttcattcat tggtatttct tttactatat
    11761 tcctattcaa cccttgaaaa ctgtacaacg ttaatacgga tcccttgaac tcgacaaacg
    11821 cataaaaata attgttcgtt gtgccatctg ttatctctac ataagtattc attgcaagcc
    11881 ctccttttga tactttgagt atagtgtatg aaacgcgtct catagcaagg agtttgtttt
    11941 gccaaagaaa agttttattc ctctttcatt attaccaaag tctcttattt taaattgaat
    12001 acattataat gatactgtaa atatatcttt attattagtg aat
//
LOCUS       DANKKG010000082        11872 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000082 DANKKG010000000
VERSION     DANKKG010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11872
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.082"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            5..2377
                     /locus_tag="PW004_001853"
     CDS             5..2377
                     /locus_tag="PW004_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726819.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQKMTNGQ
                     PMNDKRNGRSWFNQQERIENKVQSLREEIKQQEEYVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESAFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     RPTDSMKEKENDHFYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTLETPENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2438..3898
                     /locus_tag="PW004_001854"
     CDS             2438..3898
                     /locus_tag="PW004_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HDL2726820.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQIKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3909..6113
                     /gene="topB"
                     /locus_tag="PW004_001855"
     CDS             3909..6113
                     /gene="topB"
                     /locus_tag="PW004_001855"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDL2726821.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLRAEEISTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYES
                     LTPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGVLLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSIDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            6129..6278
                     /locus_tag="PW004_001856"
     CDS             6129..6278
                     /locus_tag="PW004_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726822.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKLRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            6353..6946
                     /locus_tag="PW004_001857"
     CDS             6353..6946
                     /locus_tag="PW004_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HDL2726823.1"
                     /translation="MQEKLKELLAIRDGNLSMKEARKLGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEIEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            6959..7858
                     /locus_tag="PW004_001858"
     CDS             6959..7858
                     /locus_tag="PW004_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HDL2726824.1"
                     /translation="MTPTQFAAKTRNLAKRTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTAQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIIGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            7861..8358
                     /gene="ssb"
                     /locus_tag="PW004_001859"
     CDS             7861..8358
                     /gene="ssb"
                     /locus_tag="PW004_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL2726825.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMVTLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNALQNPFGNPFGGQSIDISD
                     DDLPF"
     gene            8724..8984
                     /locus_tag="PW004_001860"
     CDS             8724..8984
                     /locus_tag="PW004_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726826.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            9429..9635
                     /locus_tag="PW004_001861"
     CDS             9429..9635
                     /locus_tag="PW004_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HDL2726827.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGQRAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            9683..9886
                     /locus_tag="PW004_001862"
     CDS             9683..9886
                     /locus_tag="PW004_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726828.1"
                     /translation="MVDKEVISEKVAEALLIPRYWTIETPLVEDAPTMLFEDLVKIAG
                     YELHYQENMETYENEYWLTKLEN"
     gene            9902..10339
                     /locus_tag="PW004_001863"
     CDS             9902..10339
                     /locus_tag="PW004_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726829.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            10332..11039
                     /locus_tag="PW004_001864"
     CDS             10332..11039
                     /locus_tag="PW004_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726830.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKHRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSCTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATIDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKRQ"
     gene            11419..11598
                     /locus_tag="PW004_001865"
     CDS             11419..11598
                     /locus_tag="PW004_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726831.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
BASE COUNT         4445 a         1968 c         2468 g         2991 t
ORIGIN      
        1 gaacatggca gaagaaaaag aaccactaga caatagtcgt ttgggaaaat ccaaaagaaa
       61 actagtacga ttgcaaaatg aattaaacga acaaattgaa aagatgtttg aacaccaaaa
      121 aatgacgaac ggtcaaccaa tgaacgacaa gaggaatggc cgttcatggt ttaaccagca
      181 agaacgaatc gaaaataaag tccaaagtct gagagaagag atcaagcaac aagaagaata
      241 cgtggaaaag cttgaaagac aagaagagct aaaggaaatg ggctataaca agtacggcgg
      301 actggatatg acgatcgata atattccaag aatcaaagaa gaaatcgaac gctttgaaaa
      361 aggtgaatcg gctttttcag ccgccacgat ccgaaaatac caacggaagt tggaaacatt
      421 agaacagctg aaagaaaaat cagaaaaagg aaaagaaaat atcctccctg aagtacaagc
      481 gatcattgac agcggacgag tgacgcaatg gaaaaaaaat ccaaccattt attttttgaa
      541 aggctatcga aaagttgctt tagaactgaa tgaaaacggg ggatttgagg aatcaaaaaa
      601 ataccaagcc acgtcggaag aggaaaaagc agctgttcgt gaattacttg aagaggctag
      661 accaactgat tctatgaagg agaaagaaaa cgatcatttc tatcgagtag aattcaacga
      721 aaatgatcca gaaaaaggat tgaaaagtta tggtggggag atcgtaacat tcgaattgat
      781 cgatcaactc aaaacattgg ataaccaata cgccgcagaa gcaggttatt ataaattcta
      841 ttttgaggaa gtgaagaacg gagaagtagt cgatcagatc cgaatggatc taggggatgg
      901 cgtcaacagt aatcaaccaa tctatttaga gttagcaaaa ttgtgcgaac aagtacctga
      961 gaaggaacaa aaacaatcgg tcaaaaatga acctgtttcg aaagaacaaa tgcagtcgaa
     1021 agaaacaaaa acaaaacaaa agggctatca aaaacgaacg gcagaagaga ttaaacaaga
     1081 agttaaatcc ctttcgcaac aggcactgga tgcggtgaaa aagcataccc attctcctga
     1141 agacgtcaaa gaacttttga actttatgtc tcgttttcca gaacgatcct ttagaaatca
     1201 attattgatt gaggaacaat ttccaggtgc aacggcttgt ttaggtcgtg ccgcgttgaa
     1261 aaaagaagga atctatatca aaaaagggga acaagggaac aagatctttg tcagaaaaac
     1321 ggtcaaaggg ttttatgaga aaggacgtgg ctttgttcga gaaacagaag ctacaccaga
     1381 agataaaaaa cgaatcgaaa gtggtcaaat cgaagtgatc aaaaaacctt actatacgat
     1441 tgaaaaagtg tttgatatca cacaaacaca gttaaaacct gaagactatc cgaaaatctt
     1501 tccgaaccgt gtctttgatt ttcaattgga taaaaaagga cagtcagaac ttcaagctgg
     1561 gattcaagcg gtagccaaca atattggaat acaaattcga gatatgacag aaagtgaagt
     1621 ctatcaaaga gaattaggac aagcacgtgg ggcgtatgtt cgtaacgaat ttactggcga
     1681 agaagaaatc gtcatgaata ctcgaaatac tcctacgcag catttagcta caagtattca
     1741 tgaattagcc catgcaagaa tgcacaaatt ttcagaatta gataccgcaa ctaaagaatt
     1801 gcaggcagaa atgacttctt atattgtgtg caaacatttt ggaatggaca cgtcggagaa
     1861 agctattcct tatattgcag catggacgaa gaatggtcag acgttggatg ataaggaagc
     1921 tgcggaacgt gggaaaatca tgaatgatgt cagccgtgtc gcaaatgaat tcattcaaac
     1981 catttctaat gaaatcaatc aatacagaga aagagaacca gagatccaac cagcagaagt
     2041 gaagcaggag cataaaaata ctttagaaac gccagagaat aagaatattg tgcccgttgg
     2101 ttcactttgg atcgacaaga aagacgggaa agtaatggaa gaagatacgg gtcgaacgtt
     2161 acatttgaag gatgcggcgt ttattgaaga tccgcagcat aatcaagtaa ttttaaaagg
     2221 aaaaagttat ggaaaagaga tccaaacggc gttaccttca gaagaatttg cgctaggaaa
     2281 tccattccga ttttcacttg agacagtgtc agaagtggca aaacgaaaaa atgaaatcta
     2341 ttatgaacaa caattgtcac aaaaagaacg tgtataaaac aagtaaaaaa gactatctga
     2401 aaaacggata gtcttttttc ttttgggagg ttttaaaatg accgagaaaa aaatgagtct
     2461 tattgatcga tgtaaacaga tcgatatagt ggattttgct cgaaacaatg gaatggctgt
     2521 agtcaataaa ggacgagatt atcgcttaga agaccatgat tcatttgttt ttgatcgaag
     2581 aaaacaacgt ttttattgga acagtcaaaa tattagtggc gacattattg aattagctaa
     2641 actttttttc atagacaaag agatccaaga ccctaagcaa caatttaaag cagccactga
     2701 ttttattcta aagaatgaag acaaaacaga acgagtggaa aatcttcatt ttgaaacaga
     2761 aaagtacaaa gatcaccccg ttgattatca accactcaca gaaaaaggaa ggaattatct
     2821 gaaggaagaa cgaaaactac cagagtggct gattgattat gcagaaaaag aagggttgat
     2881 tgccgaacta aagccaaaat atgaacggca aaatttctta gttcgagacg atcgtttaga
     2941 tcatgcggtc gcctttttat ggaaagatcc acagacaaaa gagacagtag gggcgagtta
     3001 tcaaggaacg ttcattgatt atgaacgttt tggtgagaga ggcacatata agcatataga
     3061 taaaaattcg acagcgaatc atggctttaa cctaaagatc ggtgacccta agcagataaa
     3121 attttttgag agtagtattg atttacttag ctatgctgca ctgaatcgtg atcagttaaa
     3181 cgatacgtgg ttagtctcta tggaagggtt aaaacaccat gtgatcagtc attattttgg
     3241 tgaggcagta tctgaattga gaaagaaaca agcctttcct caatccatcg agatttgtgt
     3301 ggataatgac cgtgcaggac atattttcta cgaaaaagaa caattaatgg gcgcagtcga
     3361 tccatttacg aatcagaaag tacgttgtga acgaggaatc gctaatgatt ggcaagtgcc
     3421 aaaagaatac aaaatcattt atgaagaagt cgcaaaagaa gaaaaggtca caccagaagc
     3481 tattatggcc atacacaaga cagaaaacaa tctgcaatta acgaaccaac tggtttccgc
     3541 tcacaaagta aatgcgtttt ttggccagca gctttctgtt aacgattcaa tagaagccat
     3601 taatttgaaa gatatttgta gagaagtcgc aaaagaatta aaagtttgtg aacgcgtgga
     3661 tggtacgtac gattttgatc gtttttatca agaaaaaggg gatattaatg cgcagattct
     3721 tttctcttat aaagcagaac aatactacaa aggctataaa aatcatgaac atgaatttgt
     3781 acctgaagta aaaaaggact ggaacgatca attgaagcat gagatccagc aacaagaaat
     3841 cagaaagcaa aaacgtgcca tgttgtttca acaaggcaga caacaagaaa gggagtgaaa
     3901 tcgtcatgat gaaaaccgtt attttagcag aaaagccgtc acaagcgaaa gcttatgccg
     3961 atagcttctc taaagccact cgaaaagacg ggtattttga aatccaagat cgattgtttc
     4021 ctggtgaaac tgtgatcacg tatggttttg gtcatttagt cgaattagat tcgcccgata
     4081 tgtacgatga aaattggaaa caatggtcat tagaacattt gcctattttc cctacacatt
     4141 atcactatca tgttcctaaa gataagaaaa agcaattcaa ggtggtgaaa cagcaacttc
     4201 aatcggccga caccattatt attgcgacag atagcgatcg tgaaggggaa ctgatcgctt
     4261 ggacgatcat tcaacaagta ggcgccgatc aaggaaaaac ctttaaaaga ctttggatca
     4321 actccttaga aaaagaagcc atttatcagg gattccagca gttacgagac gcaaaagaga
     4381 cctatccaaa atttgaagaa gcacaagcac gtcagatcgc cgattggtta attgggatga
     4441 acggtagccc actgtatagt ttactcttgc agcaaaaagg cataccagga agcttttcct
     4501 taggcagagt ccaaacccca acgctttaca tgatttatca attgcaggaa aagattagaa
     4561 atttcaagaa agaaccttat tttgaaggaa aatccgaagt aatcgctcaa aatggcgcat
     4621 ttgatgcgaa gcttgatccg aatgaaaccc aagcgacgca agaagcattt gaagattatt
     4681 taaaagaaaa gggggttcaa ttgggaaaac agccaggcat gattcgccaa gttgaaaccg
     4741 agaaaaaaag cgcggcaagt ccacgtcttt tttctctatc cagccttcag tctaaaatga
     4801 atcagctcat gaaagctagt gccaaagaca ctttggaagc catgcaaggc ttgtatgaag
     4861 ggaaatatct aagctatccg cgaacggata ccccttacat tacggaaggg gaatatgcgt
     4921 atctgttgga tcacttagac gaatacaagc attttttgag ggctgaagaa atttcgactc
     4981 ctattcatac accgaatagt aggtacgtga acaacaagaa agtccaggaa cattacgcaa
     5041 tcattcctac aaaaacagtt atgacagaag cgacgtatga aagtctgaca cctttacaac
     5101 aagcgattta cgagcaagtt ttaaaaacga cagtggcgat gtttgcggaa aaatatacct
     5161 atgaagaaac aacgatcttg acgcaagtcc agcagcttca gttaaaggca atcggaaaag
     5221 taccgataga tctaggttgg aagaagttat ttggcaaaga gagcgacgga aaagagaagg
     5281 aagaagagcc gctacttcct aaagtgacaa aaggagaaat ggtgacggta gatctacaag
     5341 tccttgaaaa agaaaccaag ccaccacaac cttataccga aggtacgttg atcactgcga
     5401 tgaaaacagc tgggaaaacc gtggatagtg aagaagcgca atccatattg aaagaagtgg
     5461 aaggaatcgg tacagaagcg acacgggcga atattattga aaccttgaaa caaaaggaat
     5521 atatcaaggt agagaaaaat aagcttgtgg tgacgaataa aggggttttg ctttgccaag
     5581 ctgtggaaaa agagccgctg cttacgagtg ccgaaatgac cgccaaatgg gaaagctact
     5641 tgttaaagat tggagaacga aaaggaacac aaaccacttt tctagccaat atccaaaagt
     5701 ttgtctctca cttattggaa gtcgtcccag gacaaatcca atccattgat tttggatcga
     5761 cgttacaaga agtgaaagca gccagtgaaa agcaagaagc cgcgcgtcat ttaggaattt
     5821 gtccaaaatg ccaggaacaa gaagtcttac tctatcaaaa ggtggcagct tgtacgtctg
     5881 aagcctgtga ttttaaatta tggacaacga ttgccaaaaa gaaactcaca gcgacacagc
     5941 tgaaggagat tattcaaaat agccgaacaa gccaaccagt gaaagggtta aaaggtcaga
     6001 agggttcgtt tgaagcaacg attgttttaa aagaagactt cactacaggc tttgaatttt
     6061 ctgaaaagaa aaaaacgaac ttcaagaaaa gaacacgacg cacgacaaaa taatggaggg
     6121 ttaatcttat gcgtaataca acacacaaac aacaagaaat cgaaaatcaa aaagccaaaa
     6181 ttcgttcaga acaagagaaa ttacgtgcca gtaaaagaag acttcagcaa ttaaagaacc
     6241 agcaacggat tcaaaaagaa atcgaattag atcgatagac attaaccttg ttacaaatac
     6301 actatcatga tatatttaat gtagtgtatt tgtaacaaaa ggggggaagt aaatgcaaga
     6361 gaaactgaaa gaattgttgg ctattcgcga tggaaattta tctatgaagg aagccagaaa
     6421 attaggaatc gcagcaacca ctgtacaaag attggtgaat caaggaaaat tgatcaaagt
     6481 tgatcgtggt tattatgtag aggatggtca tggaatagat gatttatttt ggcttcagca
     6541 acgtttttcc aggggaatct tctctcatga aacgacttta gatatttacc agctttctac
     6601 gaatatgccg aaattcattc acttaacttt tccccatgga tataacgttg atcgttcaat
     6661 tacaaaagag cagcagttac aatttcattt cgtgaagaaa gaagtttatg aactaggaaa
     6721 agtagaggga acttcctttc aaggaaatcc tattactatg tatgataaag agcgtacact
     6781 ttgtgatatt tggaatccga gatatgaaat tgaatatgaa atgaaattag aagcattaaa
     6841 agagtacatg gaagacccat tgagagatcc aagtaaatta agagattaca tgacaaaact
     6901 acatgtagaa aaagaaatga aataccacat gcaaagctta tattgatgga ggaaaaagat
     6961 gacaccgaca caatttgcag caaaaacaag aaatttagca aagaggacag gattaaatgc
     7021 acagttagta caacggcatt ttatgatgga tcgtttaatt gaacaaattt ctgaatctaa
     7081 gtataaaaat gatttcattt tgaaaggtgg ctttttatta ggatcaaaat atggaatcga
     7141 tagaaggacg acaattgata tcgacacgac atttagaaat agcaaattaa ctgaagaaaa
     7201 attgaagagt atttttaatg atttaactag gaaacccacg aaagaaggta ttgttttctc
     7261 tattcaagga atgaaggaaa caagagaagc cgatttttat cctggatttc aaattaaatt
     7321 ggtggctaat ttagagaaag tacgtgtgcc atttaaaatg gacgtgacaa caggagattc
     7381 tatttatcct gaagtagata cacactatca ccaattaatg tttgaagata aaaaggtgga
     7441 gattccagct tatccaacag cacaaattat tgcagaaaag ctaagtgcta cgttcagctt
     7501 tggcacagat aattcgagag caaaagatta ctacgactta tttaccattc cgaaactaga
     7561 aaatatcgat acgaaagagt tatacaaaag tgttcgaaat acatttacaa aacgtgggga
     7621 tacaaaaccg ttgtcctttt attatgaaag agaaatggat aagatcatag gaaatgacta
     7681 tttggaaaag cagtgggaaa aatatcgctc agttaacacg tttgcgaatt ccatttcatt
     7741 tacagataca gtcaacgaaa ttgatcacct aatgaaaagt gtgatcaaaa ttgaaaatca
     7801 agaaagaaaa cggcagttta aaagatcaca acaaatgaat caagaaatgg agagataaag
     7861 atgatcaata atgtaacttt agtaggacgc ttaaccaaag atccagattt acgttatacc
     7921 gcgagtggta ctgcagtagc gacttttacc ctagctgtca accgaaattt cacgaatcaa
     7981 aacggcaatc gagaagctga ttttattaac tgtgtgattt ggcgcaaacc agcagaaaca
     8041 atggtgacat tagctaaaaa aggtagcttg attggtgtag ttgggcggat tcaaacgcgg
     8101 acttatgaca accagcaagg acaacgtgtg tatgtgacag aagttgtggc cgataatttc
     8161 caactattag aaagcaaagc agcgacagaa agccgtgcgc acgctgatca atcgtccacc
     8221 agtccaagca ccaccacttt tgaacaacgg gatacagcga cgcctaataa caatggattg
     8281 aacgctttac agaatccatt tgggaatcca tttggtggac aatcaatcga tattagcgat
     8341 gatgatctac cgttctaagt agcattttct attggttgca tttgctacgg tttatgttat
     8401 aatttagtca acaaaaagaa ttttcaatct ataaccaaaa accactagct ggcgggctag
     8461 tggttttctt ttacgtttct attataagaa ttaacgggta ggcttactgt ctttcttggc
     8521 aaatttacaa tcaagccatt tgcagatata gtgcgcggct acttctgcca tgattgcaaa
     8581 aacaaaatca aaaagaattt tcaaacctat aacctcctcc ccttgccaga ttaggccggg
     8641 gtagcagaca gcaagactat tatagcataa ttgaaatatc caaatgattg gatttttgct
     8701 ataattttag cgaggtgatt gtgatgaaca aatatgttgg tttgcttgat aaaatccgag
     8761 taataaaaac gcaaccgtta ttggttcgct ttactttaca aacgattcat gaatcgatta
     8821 attgtgtagt tgcagatatt gaaattatcg ataaattgct aattatggat gatggaaaat
     8881 acaatattgc cgtcacaggt cacttcaata aacgaaatca gctagttatt gctagcatgt
     8941 atgttagaaa tcccgatcat tttacgcgat cgatgggcat ataaaaaaag aagagttgca
     9001 atcctaatgt aggactgcag ctctttttgt tttacataga aacaagaaaa atgaaaaggc
     9061 ctttttagga aggcgcgagc gccttctcaa ttgaaaaacc tctctcactc caaccgccac
     9121 cgccgttcga gaagaaagga agtgaacgtt gcacgttcac taagataaag ggtcttgcaa
     9181 agcaagacaa aatagaaaaa acttcctctc accaaaaggt tcgttcctgt tcgttatctc
     9241 tttattgatt tgccagaccg gcaacaagtt gccttttatc accaaaaagc gacaaggaca
     9301 agggtaaatg gcacgctgcg cgcccttgtc cttgtcgctt ttcgttgatt cattctggcc
     9361 aacaaatcaa taaagaaaga actaacgaaa ggaagtaaaa cctttcctaa gaggaggcag
     9421 aatcaatcat gcgaaagaaa tttgattatt ggggtgttcc cttcagcgaa ttattcccaa
     9481 actatcatgc accacatact gtcgaatgcg attgtggaca aagagcgaaa tgtatcaaaa
     9541 gctatcgttt gtatcaatgt ccaacatgcg gaaaaaaata caccttgtct tatggcgatt
     9601 atattttgat tgatgagaaa ggaaagaaac gctgatgaaa acataacata aattattgac
     9661 tgtaaaagaa gtcgttcaaa ttttggtgga caaagaagtc atttctgaaa aagtagcaga
     9721 agctttactt attccgcggt actggacgat agaaacccct ttagtagagg atgcaccgac
     9781 tatgctattt gaagacctag tgaagattgc aggatatgaa ctgcattatc aagaaaacat
     9841 ggaaacttat gaaaacgaat attggttaac caaattagag aactaaaaag gagaaaaaaa
     9901 tatggctcaa aattactatg acgaatttgt caaattacct ttggataaaa tggcacaaaa
     9961 aatggaggat atgacattcc tataccatga gacacgtgtt ccaaaaaaac attataagga
    10021 aagattgtcg gtagctgtgg aagaaatgat tgaatcaggt gtcgaaatga atctcattgc
    10081 gacgtactat cgaacactgg aagaactgaa aaaacaaaat gcaaaatggt ttttccaagc
    10141 cttactatgc ttggaagtag gtgtcaaacc aagcactatt aaaccttctg aatatcaagc
    10201 tttagaacta acgtatggga aatttgtcga aacgaaaaaa gccaagactg tttcaagtga
    10261 atggttagat tattttgaaa atatcaataa gtatggcgcc tattacacca tgaaaaagga
    10321 ggacaacgaa aatgaataag gtgatttatc aactaacaac ggaagatgtg tcacactttt
    10381 taacaaacaa agaacatttg aatgaagtaa tcagacggat tcaaaaagac tttagttgta
    10441 tggaagaaat gaagtacttt attcaaagag tcgaagaaca attgtatttg gaaagtgaaa
    10501 cgagtcaaac gaaggtcaca gtcagcaata attatttaga cgtgtataaa catcgtcctg
    10561 aaacggtaaa aaaaggaacg gagcttctac gactagaaac agaaaaatac atcatgaatg
    10621 tttacctaga aaggagtgaa ctggtcgaaa actttgtcag ctgcacgatt tgcaggaaac
    10681 actattactc agaatcagaa tattattcaa tttttgagag catggaatta atggaagaag
    10741 aaatggaaga gctaaacagc aaaaatgcga cgatagacga ttattatctt gtatttacta
    10801 gatttttgac gtgtggaacg gaatctatcg tgttaatggg aatggattat gaactcgctg
    10861 tacccgaagc atttttgagg gaatatgtag aagaagtaga taacccaggc gacgactggt
    10921 acacgacttt aactgatttt ttagatacct acacttacga cgatacagaa gaaatccaaa
    10981 atgaacttga ccgtaaacga atgccatatc aacgatttga aaggaaaaaa aggcaataat
    11041 catttatacc gaaaagtgag aatgtgtgaa ataagaacaa aaaccttgaa attaagggaa
    11101 tggttcttat ttcgcaccaa tcgctccaac cgcccccgcc gcttaggtgg tgcgaaataa
    11161 gcgggcaaag cccgataacc taagagaaag aaggagatcc atatgaataa aatcattcct
    11221 ctgttgttcg tactgtgtat tggcgagata cttatgcgtc atggtgcagc tgtacattta
    11281 aacttcgtag tattcttagg aatactagta ttttgtttga ttttttggcg ctttgtgtta
    11341 gcccttgtga gtctactatt caagttgatt gcagccatat ttttcatagg gttatttcga
    11401 tgaaaggaga gagtactaat gccaattgca ataggaaata aacggttacc cgtgacatta
    11461 gatgaaaaga gacaaaaaga attgcagcaa ctaaagcaga agtacggaaa aagtgaatct
    11521 aagattatgt gtattgcgtt agatctattg atcgcgcaag aaaaagcagg atttgatata
    11581 ccagcactaa aaaagtgacg tcacttttca tttgaagaaa caaaagtgat agcacttaat
    11641 tgttcctact atttagaata ttttctatgt cctttttatt ccattttgta tcaataacta
    11701 tgaaatcttt aaagtatcca gatcaatttt tttcttacct gattctaaag attttgtttt
    11761 cttaaacata ttatgatttc ctttcgacta ttttcatata aatgttaaca tagtttatgg
    11821 attcatggaa tgttaatcta atttaataga aaaggataat taagtatata cg
//
LOCUS       DANKKG010000083        11586 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000083 DANKKG010000000
VERSION     DANKKG010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.083"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            93..3320
                     /gene="fss3"
                     /locus_tag="PW004_001866"
     CDS             93..3320
                     /gene="fss3"
                     /locus_tag="PW004_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HDL2726832.1"
                     /translation="MYKKQKKKLCVLTILSLAVQSIVGAIAPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCVEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIADYQKKPSFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENTANIDYRVN
                     GNQLIISPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTVKIDVE
                     MEGSLKIKKVDKESGDIIPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTATIKAGETISVTSKNTREKGQIILDKTGIETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGHETQGKAEFKRAEYTL
                     FTVKDGKAVKWTENFKPELLKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDSEKNAVITRDVAAKEQVIRFGFDFFKFAGSTTGTAETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDYTKSDYIFTITEKGQKQPIKTVTVPYGNLTNENFSVSLNR
                     LMLYDLPEEEDSLTSLATWKEGDKELTTLDSTELVDKLSYNLHEIKEDWYIVAQAIDV
                     DATKAAQEKDKKAKPVAIAETTATLANKEKTGTWEILHKLTVEQVLDKTIVLFNYVYE
                     SKEAYEAGDEPVAKDASLNNQAQTVNCMVERHVSIQTKAHLEEGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALFPDGTSKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYDKDGNINGKHNEDLKEKSQTLTPKKVPTTPSTPEQSEMPTVP
                     SGSQESSPTVKAFPQTGEKTSNVLLFIGFSLIFATAGYYFWNRRN"
     gene            3432..3746
                     /locus_tag="PW004_001867"
     CDS             3432..3746
                     /locus_tag="PW004_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDL2726833.1"
                     /translation="MELKFVVPDMVETFGKLTYGGEGEVLSEGYGRNTTIVGRSYHLF
                     SNKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLIAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            3759..4133
                     /locus_tag="PW004_001868"
     CDS             3759..4133
                     /locus_tag="PW004_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDL2726834.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQLNQGKPTNQNEGKK"
     gene            4134..4487
                     /locus_tag="PW004_001869"
     CDS             4134..4487
                     /locus_tag="PW004_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HDL2726835.1"
                     /translation="MKNMEINVEHILDCLDQFGKGELTEEQLTNALTYDEKMFLIMHQ
                     GLLEVGNDAKEDFDVLDWLGEEESFFMVVEVNESLCRQAESVLEEIDVEMPDAIERFL
                     KQLVETKQLPVVVND"
     gene            4556..5572
                     /locus_tag="PW004_001870"
     CDS             4556..5572
                     /locus_tag="PW004_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HDL2726836.1"
                     /translation="MYKGHRIRAGDQHLIYHFVLGWLIFLFIGWMSVFYLQELRQFDI
                     SKVSFSTSEIGWSMKDVICLLGSLALSGSMMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQGEGFFKDLNSSWTKESISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LENKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIEEIVAENGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIQALLKSDAELFILDPKNADLADLGTIMPHVYSQK
                     EEISECVEDFYERMIARSRSMKEMPNYKTGENYAYLGLPPNFLIFDEYVAYMGANRFP
                     TSIE"
     gene            6166..8040
                     /gene="ltrA"
                     /locus_tag="PW004_001871"
     CDS             6166..8040
                     /gene="ltrA"
                     /locus_tag="PW004_001871"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HDL2726837.1"
                     /translation="MNVETDEVQGSGKNVGKSVMEVPRLNTKIRYWEYYDLQETFDQL
                     FTQSRNGKKFYQLYELIISENNILLAYRTIKANKGSSTPGTDSFTIDNYKEMNQAEFI
                     HLILSHLENYKPKSIKRVMIPKPNGEKRPLGIPCMIDRMIQQMFKQILEPICEAKFYE
                     HSYGFRPLRSAKHALGRIMYLINISKMHYAVDIDIKGFFDNVNHRLLIKQLWNIGICD
                     KQVLAILSKSLKSPIQGEGIPSKGTIQGGIISPLLSNVVLNDLDHWVSKQWHTFETKY
                     PYTKGYNKFRALRDTNLKQGYIVRYADDFKIMTNDYPTALKWFHAVKLYLKDRLKLDI
                     SNEKSKIVNLRKCKSEFLGFAICVKQKGKKWVCNSRISNKKKDQIKEEIKQRIKDIQK
                     SPTAQNALLFNALVLGVHNYFRYATHINLDLNRMNYVLSFTTYNRLSNCSTRCYPNKA
                     SPTYKKFYSTTMKTYKVAGVYLYPLCDVSTKTIYGFNTEDTPFTPFGRQRLEHKSLQS
                     LVFQELRKLMESKIPNRTVEYLDSRISRYSMKSGKCEITKQFLPAKAVHCHHYLPKSL
                     GGDDKFDNLRIIHKDIHLLIHTTNKMIIDHYVHELKLVPEQIAKINLYRKMCNLQNIQ
                     "
     gene            8166..8522
                     /locus_tag="PW004_001872"
     CDS             8166..8522
                     /locus_tag="PW004_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HDL2726838.1"
                     /translation="MILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRV
                     ALGRMSELGYSMMFGEVDKNFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREIS
                     NIVGLTVHTERENNSV"
     gene            8567..9709
                     /gene="dcm"
                     /locus_tag="PW004_001873"
     CDS             8567..9709
                     /gene="dcm"
                     /locus_tag="PW004_001873"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HDL2726839.1"
                     /translation="MTFLDLFAGIGGFRLGMEQAGHQCIGFCEIDEFARRSYKAIHDT
                     RKEVEMHDITSVSDEFVRSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     ATLLQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQSRERI
                     FLIGHSRDACTEQVFPIIGSSSTSDQNIRNLVNINPSNRGMGGQVYGSDSVAPTLTGD
                     EGIKIALPVKDGISVAGMLSGNFEQGNRIYEVTGTAPTLSTKQGGTKIMIRKNETEYQ
                     EVKPGDSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKGVKAYDL"
     gene            9699..9988
                     /locus_tag="PW004_001874"
                     /pseudo
     CDS             9699..9988
                     /locus_tag="PW004_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10244..11428
                     /locus_tag="PW004_001875"
     CDS             10244..11428
                     /locus_tag="PW004_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDL2726840.1"
                     /translation="MAQRNLDYRLLKERRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     IASTKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDVRKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYYAIQDLL
                     KGRSIESTTFSIINRYLRFADKVDGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     IV"
     gene            11425..11538
                     /locus_tag="PW004_001876"
     CDS             11425..11538
                     /locus_tag="PW004_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HDL2726841.1"
                     /translation="MKRGILMEIVKDMLLVLGGSMLGVTVMCLMNASAAAD"
BASE COUNT         3889 a         1984 c         2576 g         3137 t
ORIGIN      
        1 tagtcaataa atattttaaa aagactttat aaacaaagat gttcaagcga gcatcttatt
       61 tttttgtgcc taatttgaaa ggagaacatt tcatgtataa aaaacaaaaa aagaaattat
      121 gtgtattaac tattttgagt ttggctgttc aatcaattgt aggagcaatt gctcctacga
      181 ttgcttttgc tgatgaaatt acccatccac aaacggtaac cgtagaacta gatttagcac
      241 atcaatatgc tgttgaagga acttttagtg atggtcgtcc aatgtcagaa gtcactgttc
      301 cgcattatgc agtgtacaac ggcgttaaac aagatatttt ctgtgttgaa ccaggagtcc
      361 cgatttacaa tgagttcact cctggatatg agaagaaccc gttaccagat atgtccgata
      421 aagccaaatt agtttccgtt ttatggaaaa aagtaggtac agatgcagat actcatattg
      481 tggcacagaa gatgatctgg caagaagtta atgggtatac ccttcactca ataaaacgat
      541 cagatggtag tgccgtaaat atcgcagcca ttgaggcaaa aatcaatcaa gccattgccg
      601 attatcagaa gaaaccaagc ttccataata gtacggctaa aacagtatta gggcaatcga
      661 ctactgtgac ggatacgaat aatctgaatt tgtcagagtt cgatgaggtt gtggaaaata
      721 ccgcaaacat tgattatcgt gtgaatggca atcagttgat aatcagccca aatgccaatt
      781 caaaagaaag tggtgtgttg acgcttaaga aatctgctgg taccggaaca cctgtcgcct
      841 ataaaaaggc tggccaacag actttaatgg ccggggcgat cgataagccg aacacttata
      901 cggtcaaaat tgatgtggaa atggagggtt ctttgaagat taaaaaagtt gataaagaat
      961 cgggtgatat tataccagga acggttttcc atttagactt tggaaaaact ttacccgcaa
     1021 aagacgtgac caccgataaa gaaggcattg cgaccttaga tgagattcct catggtacaa
     1081 aggtaaccat tactgaaaaa tcggtgccgg cgccttatac gattgatacc acacccatga
     1141 ctgccacaat taaagcaggt gaaaccattt cagtgacttc aaaaaatacg cgagaaaaag
     1201 gtcaaattat tctggataaa accggaattg aaacagggag tgatctttgg aacgataatt
     1261 actctttagc gggcaatacg tttgccattc gcaaggatag cccaactggt gaaatcgtcc
     1321 aagaaatgac cacggatgaa aaaggtcatg cagaaacacc aaaagagatt gccaatgcgt
     1381 tggaattggg aacctactat gtgactgaaa ccaaggctag tcatggcttt gtgaatactt
     1441 tcaaaccagt aaaagtcgaa ttgaattatg tcaatcaaac agtggctctt gttaccagta
     1501 acgtaaaagg gcaaaaccaa gaaattactg gtgaaaccac tttgacaaaa gaagacaaag
     1561 ataccggtca tgaaactcaa gggaaagctg agtttaaaag agctgaatac accttgttta
     1621 ctgttaagga tggaaaagcg gtcaaatgga ccgagaactt taaaccagaa ttgttgaagg
     1681 gaacgaaagc ttctgatgaa accgtaactt tgactttaga tgaaaagaat caagttgccg
     1741 ttaaacactt agcgattaat gagtattact ggcaagaaac caaagcgcct gaaggatata
     1801 ccttggatga aacaaagtat cctgtatcca tcaaaaaggt ggatgatagc gaaaaaaatg
     1861 ctgtgattac tcgagatgtt gcggcaaaag aacaagtgat tcgctttggc tttgatttct
     1921 ttaaatttgc tggatcaact actggcactg ccgaaactgg cttcaatgat ctaaccttta
     1981 aagtgtcgcc attggaagga accaatgaaa tcaccggtgc cgaagatgaa gcgaccacag
     2041 cttataacga gcaattaggg tttgatggtt atggcaagtt tgaaaatctt ccttatgggg
     2101 attatttact tgaagaagta gaagccccag aaggttttca aaagattacg ccattagaaa
     2161 tccgttctac ttttaaagaa aataaagagg attataccaa gagtgattat atctttacta
     2221 ttaccgaaaa agggcaaaag caaccgatta aaacggtaac ggtcccttat gggaatctaa
     2281 ccaatgagaa tttctcagtc agtttgaacc gtttgatgct ttatgatttg cctgaggaag
     2341 aagatagctt aacttctctt gcgacttgga aagaaggaga taaagaattg actactcttg
     2401 attctaccga gctagtcgat aaattgagct ataatctaca tgaaatcaaa gaagactggt
     2461 atatcgtagc tcaagccatc gatgtagatg ctacaaaagc tgcccaagaa aaagataaaa
     2521 aagccaaacc ggtggcgatt gcggaaacaa ccgcaacttt agcgaataaa gagaaaacag
     2581 gaacttggga gattctgcat aaattaactg tcgaacaagt tttagacaag accattgtct
     2641 tattcaacta tgtgtatgaa agcaaagaag cctatgaagc tggtgatgag ccggtagcga
     2701 aggatgctag cttgaacaat caagcgcaaa ctgttaattg tatggtggaa cgtcatgttt
     2761 ccatccaaac caaagcgcac ttagaagaag gttctcagac ctttactcat ggtgacgtgg
     2821 tagatatgtt tgatgatgta tccattaccc atgatgtact agatggctca aaagaagctt
     2881 ttgaaacaat tctgtacgct ttatttccag atggtacaag taaagaaatt tggaaatctg
     2941 gcaagattga ttacgaagtg aatgacaaag aatttaccaa aaccgtactt gcggaaaaag
     3001 tggatacaag taaatatcca gaaggtacaa gttttacctt caaagaaatt aactatgaca
     3061 aagatgggaa tatcaatgga aaacacaatg aagatttgaa agaaaaatct caaactttaa
     3121 cgcccaaaaa agttccgaca acaccaagta caccagaaca atctgaaatg ccaactgttc
     3181 caagtggctc tcaagaatct agtcctacag tgaaggcatt ccctcaaacc ggggagaaaa
     3241 cttccaatgt gctactgttc attggcttta gcttgatctt tgcgacggct ggctattatt
     3301 tttggaatcg tcggaactaa ggtgataaga tgaaacatgt aaatagtaga aaacaatacg
     3361 cgcgaccgcc tcctaaaaaa gtggtcgcgc tatttttgta ttcaaaacta acaatgaaaa
     3421 gaggaaaaaa tatggaattg aaatttgttg taccagatat ggtcgagacg ttcggaaaac
     3481 taacatacgg tggtgaaggt gaagttctat cggaagggta tggtcgaaat actacaattg
     3541 ttggtcgaag ttaccatctg ttttcaaata aacaacgggc cgatgatatt gaagtggtgg
     3601 tagctgcgga agccggcgaa aaggattttg accaagatca accactaaaa gctgtaaatc
     3661 cccacttaat tgccaaaggc tatgaaattg agaatcgtgg gtttaccgat tacgtgttgt
     3721 atgttgatga tttagtgaaa gcataggagg aaaagaagat gagattaaca gaaggtattg
     3781 tcgtagattc aggattaacc tttggcaagt tgcgtttttc cgcattacgt cgagaagtac
     3841 gcaagcaaaa tgaggacgga acggttagta acgaggtaaa agaacggacc tataatttga
     3901 aatcctccgc gcaaggtcga atgatccaag tcagtattcc agcaaatgta cccttacgcg
     3961 agtttgctta tgatgcagag gtagagctgg tcaatccaat cgtggatacg gtcgccaatt
     4021 atgtttttcg ggaaggaacc accgttaatt ggtttatcaa ggcagatgat ttagtcttga
     4081 aacgacaact gaatcaagga aaacctacga accaaaatga aggaaagaaa taggtgaaga
     4141 atatggaaat taatgttgaa catattttag attgtctcga tcaatttggc aaaggtgagc
     4201 taacggagga acaactcact aacgcattaa cctatgatga aaaaatgttt ctgattatgc
     4261 atcaagggtt actggaagta ggtaacgatg ccaaagaaga ctttgatgta ctggattggt
     4321 taggggaaga agagtctttc tttatggttg tcgaggtaaa tgaaagttta tgtcggcagg
     4381 cagaatctgt tttggaagaa atcgatgtgg agatgccgga tgccatcgaa agatttttaa
     4441 aacaattagt ggaaacgaaa caacttcccg tagtagtaaa tgactagcat aactttttga
     4501 aattgcccaa tgaaatccta ttgggctttt tcgactggag gtggaaacca tcaaaatgta
     4561 caaaggacat cgcatacgcg caggagatca acacttgatt tatcactttg ttcttggctg
     4621 gctaatcttt ttgttcattg gttggatgag cgtcttttat ttacaagaac ttagacaatt
     4681 tgatatttct aaggtatcat tttccactag tgaaattggc tggtctatga aggatgtaat
     4741 ctgcttgcta ggaagtctcg ctctttcagg aagcatgatg ttactttata tacacttttt
     4801 tcaggatcat tggcgaagtc tttggcatcg acaaaagtta gctcggatga ttctagaaaa
     4861 ccattggtat gaagtaaagc agactcaagg tgaaggtttt ttcaaagatt tgaatagtag
     4921 ttggaccaaa gagagtatca gttacttccc caaaatttat tatcgaatga aagatggctt
     4981 actttctatt cgcgttcaaa tttcactggg aaaatatcag gatcagctat tgaagttgga
     5041 aaataagtta gaaagcggac tgtattgtga gttagtagaa aaagaactca aagactccta
     5101 tgtggaatac accttgttat acgatatgat tgccaatcga attgggatag aagaaatagt
     5161 ggcagaaaat ggggcgttgc gattgatgaa aaatcaagta tgggcgtatg attccttgcc
     5221 gcatatgtta attgctggcg gcacaggtgg tgggaagacc tatttcttac tcaccattat
     5281 tcaagcacta ttgaagtcag atgcagaatt gtttatcctc gatcccaaaa atgcggacct
     5341 cgccgattta ggtacgataa tgcctcacgt atactcccaa aaagaagaaa tttctgaatg
     5401 tgtggaagat ttttacgaac gcatgatagc tcgtagtagg tcaatgaaag aaatgcccaa
     5461 ctacaaaaca ggagagaatt atgcgtatct cggacttccg cccaactttt taatctttga
     5521 tgaatacgtg gcttatatgg gtgcgaatcg ttttcctaca agcatcgaat agatgtgaaa
     5581 tggaagccta ttgagattat caatagtgac aactaatgtt ttgagagatg gaatgaaagc
     5641 cttcaagttg agctgaaaca tccccgctaa tactcctaca tgctctaatc aatgaagtag
     5701 attggttttg gtatgaagct aggtgaagtc gtctgaaatc tgcctaagtg cttgagcata
     5761 aggctgacta tagggcgggc ggctgaaaaa gataactatg gtaagaatgt ctgaaaatga
     5821 ctgacgaatc tgcgaatgta cgggtctaaa aggctgatct tattgaaaga taggaacata
     5881 cggacgtatg gtgagatacc gacaagtaag gttccatgat atgaaaatcg ggatagctaa
     5941 cgaagaagct ataatttcac aggctcatag tagataccta aggttataca tgatagctaa
     6001 aaacattgga acgatgtaag cagaaaacta tcaattaaat gactacttct agataggtgc
     6061 atataagtaa taacgaaatt gctttgtttc tgtgaaagta agggcacagt accgatgaag
     6121 cgtctaacaa atgtggaggg atagccctaa gtctatttaa taacaatgaa tgttgaaaca
     6181 gatgaagttc aaggatctgg taagaacgtg ggaaaatccg ttatggaggt gccacgcttg
     6241 aacacaaaaa tacgttactg ggaatattat gatctacaag aaacattcga tcagctattt
     6301 actcaaagta gaaacgggaa aaagttttat caattatatg agttaatcat ttctgaaaat
     6361 aatattcttt tagcataccg tactatcaag gcaaacaagg ggtcttcgac accaggaacg
     6421 gattccttca caattgataa ctataaggaa atgaaccaag cagagtttat ccatcttatt
     6481 ctcagtcatt tagaaaatta caaaccaaaa tccataaagc gagtaatgat acctaaaccc
     6541 aatggtgaga aacgcccatt aggaattcca tgcatgattg atcgaatgat ccaacagatg
     6601 ttcaagcaga tactggagcc aatatgtgaa gctaagtttt atgaacatag ctacggtttc
     6661 agaccattga gaagtgcaaa gcatgctcta ggtcgcatta tgtatctcat aaacatcagt
     6721 aaaatgcact atgcagtaga cattgatatc aaaggtttct ttgataatgt aaaccaccgt
     6781 ttactgataa aacaattatg gaatatagga atttgtgaca aacaagtctt agctattctt
     6841 tcaaaatctc tgaaatcacc cattcaagga gaaggtatcc cgagcaaagg aacgatccaa
     6901 ggaggtatca tttctccact attatctaat gttgttctaa acgacttaga ccattgggtc
     6961 tcaaagcagt ggcatacctt tgaaacaaag tatccctata ctaaaggtta caataaattt
     7021 cgtgctctaa gagatacaaa tctaaagcaa ggctatattg tcaggtacgc agatgacttt
     7081 aaaatcatga ctaatgatta tccgacagca ttaaaatggt tccacgctgt caagctttac
     7141 ctaaaagaca gacttaaatt agatatttcc aatgagaagt ctaaaatcgt caatttgcgt
     7201 aaatgtaagt cggaattttt aggattcgct atttgcgtga aacaaaaagg gaaaaaatgg
     7261 gtttgtaatt cacgtatttc taacaaaaaa aaggatcaga ttaaagaaga gattaaacaa
     7321 agaatcaaag atattcaaaa gagtcctaca gcacaaaatg ctctcctgtt taatgctctc
     7381 gtgctcggtg ttcataatta tttcaggtat gccactcata ttaatttaga tttgaatagg
     7441 atgaactacg ttctttcatt cactacttac aaccgattat caaattgtag tacaagatgt
     7501 taccccaaca aagcttcccc aacttacaaa aagttttact caacaacgat gaaaacgtat
     7561 aaagtagctg gagtctattt gtaccctctt tgcgacgtat ctacaaaaac aatttacgga
     7621 tttaatacgg aggatacacc gtttactccg tttggtcgtc aaagattgga gcacaaaagc
     7681 ctacagtctc ttgtgttcca agaattaaga aaattgatgg aatcgaaaat accgaataga
     7741 acggttgagt atttagatag tagaatatct cgttactcaa tgaaatcagg caagtgtgag
     7801 attacaaaac aatttttgcc agcaaaagcc gttcactgtc atcactattt gccaaaaagt
     7861 cttggtggag acgacaaatt cgataacttg agaattattc ataaggatat ccatcttctg
     7921 atccatacaa caaataaaat gataatcgat cactacgtgc atgaactgaa actggtgcca
     7981 gagcaaatag caaaaatcaa cctttatcga aagatgtgta atctgcaaaa cattcaatag
     8041 aaaataaata gatggaacgc cgtatgaggt gaaagtctca cgtacggtgt ggagcggggg
     8101 aaaagatgga gattatatca aattcttacc tatcgctata gatgttaacg acaaaagaaa
     8161 gtgcggtgat tttgaataag cttaaacaaa tcgtgatgtt aggccgtcaa tctggtttct
     8221 ttctgattct agcttgtcaa aggccggatg cgaaatatct aggagatggg attcgcgatc
     8281 agtttaactt ccgtgtggct ttgggtcgta tgagtgagct tgggtattcc atgatgtttg
     8341 gagaagtcga taaaaatttt tttatgaaac gcatcaaagg acgaggttat gtagatacag
     8401 ggggcagtgt gattagtgag ttttatacgc cacttgtacc aaaagggtat gatttcttaa
     8461 gggaaattag caacattgtt ggattaaccg ttcatacgga aagagaaaat aatagtgtgt
     8521 agcctaggaa tatcaataat cctgggcttt tttattgagg tgatttatga catttttaga
     8581 tttattcgcc gggattggtg gctttcgctt aggtatggaa caagccggtc accagtgtat
     8641 cggtttttgt gaaattgatg aatttgctcg gaggagttat aaagcaatcc atgatacaag
     8701 gaaggaggtg gaaatgcatg acatcacaag tgtatcagac gaatttgttc gatccctcgg
     8761 accagtggac atcctttgcg gcggctttcc gtgccaagct ttttcaattg cgggaaagcg
     8821 gcaaggattt tccgatactc gaggtacttt attctttgaa atcgctcggt tcgccactct
     8881 tctccaacct aagtttctat tccttgagaa cgtccgggga ttgctcaatc acgaaggagg
     8941 ggctacgttc gagacgatcc tccgaacgtt ggatggattg gggtatgatg tggaatggca
     9001 agtgcttaac tcaaaggcct acgttcccca aagccgtgag cggatcttcc ttatcggaca
     9061 ttctcgagat gcatgtaccg aacaagtatt tcctatcatt ggatcgtctt caacatctga
     9121 tcaaaacatc agaaacttgg taaatatcaa tccttccaat cgaggaatgg gtggccaagt
     9181 ctatggttca gatagcgtag caccaacctt gaccggtgat gaaggaatta aaattgcctt
     9241 accggtaaag gatgggattt ctgtagcggg tatgttgtcg ggaaattttg aacaaggcaa
     9301 taggatttat gaggtaaccg gaacagcccc cactttgtca acgaaacaag ggggaacgaa
     9361 gattatgatt cggaaaaatg aaacagaata tcaagaggtg aagccagggg acagtgtgaa
     9421 cctggctttt cctaattcca caagtcgccg aggtcgtctg ggaaaacaaa gtgtgcatac
     9481 cttactaaca ggagaccaac aagcggtagt cacggatcag taccagattc gaaagttaac
     9541 acctagggag tgctggcggc ttcagggctt tccagattgg gcttttgatc gagcttctca
     9601 agtcaattca gatagtcagt tgtataaaca ggccggaaac tcagtaacag ttccggtcat
     9661 ttttgatatt gctagaagat taaagggggt aaaagcatat gatttatgac gagttgattg
     9721 gagaaatcta ctggatgatt ggcaagattc aggcagatcc gaaattggaa gaagaactgt
     9781 accggctcaa ttttgatatt cgcaaaaacg gggtgaaagt ccaggtgatt cgtatgtagt
     9841 ggatgaggaa acggatgctc gaatggagct caatcaagtc atcggtgaat tggaacgaat
     9901 tgcggacctt gccaaagaac ctgatatccg ccaatacttg tttgagataa aagcagagtt
     9961 ggaaattgag ggtatcaccg cggagtaata gggaaataac ccactgatag gcactgaccg
    10021 cgggggattt gcgaagcaga aatcctccgt ggtgctgtgc ctgttcatgg acggcggcag
    10081 ccggccatga tgtctcaccc cccgtatcta acaggggggt acaaaaacac aaaaaacaag
    10141 caagtaacca taaaaaatgc ttgttgtatc aaggatatta agtaatttgt ataggtgtta
    10201 actcatttgg aaaagttaac accttgaatt tggaggtgaa aacttggcgc aacgaaattt
    10261 agattatcgg ttattaaaag aacgacgcaa cgaatatggc gtttcacaaa ataaattagc
    10321 tactgcttgc ggacttagcc gcccatacct aaatcagata gaaaacggtg gcgtaatcgc
    10381 ctctacaaag accatgagaa aaatttttga tcaactggaa agtttcaatc ccgatttacc
    10441 tttaacgtta ctatttgact atgtaaggat tcgctttccc acaacggatg tgcggaaaat
    10501 tattcaagaa attctccatt tgaaattcga gtatatgctt catgaagatt acgcctttta
    10561 ctcctatcaa gaacaatatg tcatgggcga tattgtagtg atgttatctc atgaagaaga
    10621 taaaggtgtc cttttggaat taaaaggtcg tggttgccgg cagtttgaaa cttttttact
    10681 cgcccaaaag cgtagctggt acgacttttt tgaagattgt ctaaaagccg gtggcgtgat
    10741 gaaacgcttg gatttagcaa tcaatgatcg agtggggcta ctggatattc ctgatttaac
    10801 gaaaaaatgt cagaaggaag aatgtatctc cttgtttcgt accttcaaaa gttaccgttc
    10861 cggggaactg ttgaaagctg atgaaaagga tggtatgggc aataccttat atattggcag
    10921 cctcaaaagc gaggtttatt tttgtttgta cgaaaaagat tatgaacaat atatcaagtt
    10981 agggattcca ttggaccaga ccgaaacgaa aaatcgtttt gagattcggt tgaaaaatga
    11041 ccgtgcttat tatgcgattc aagatttgtt aaaaggtcgt agcattgaaa gtaccacgtt
    11101 ttccattatc aatcggtatt tgcgatttgc ggataaagta gatggaaaac ggcgaaccaa
    11161 ttggcccttg aatgaacaat ggggtcgttt tattggacgg aatcggaaag agattcaatt
    11221 aacttctgaa ccgaaaccgt ataccattga acgaacatta aattggctag gtagacaagt
    11281 cgcccctacg tggaaaatgg ctaaggagtt ggatcgattg aatcaaacaa cgtatatcca
    11341 ggatatggta cagaatgcgc gactctcgga ccggcataaa aagattttgg agcaacaaag
    11401 tatggcaatc gaaaacttga ttgtatgaaa agaggaattt tgatggaaat tgtaaaagat
    11461 atgttattgg tacttggtgg cagtatgctt ggtgttaccg tgatgtgctt gatgaatgca
    11521 agtgcagcag cggattgagc aatggaaaag aaaggaaagt gacaaaatga atttcggaca
    11581 aaattt
//
LOCUS       DANKKG010000084        11504 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000084 DANKKG010000000
VERSION     DANKKG010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.084"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            20..526
                     /locus_tag="PW004_001877"
     CDS             20..526
                     /locus_tag="PW004_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL2726842.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRMLIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            543..1442
                     /locus_tag="PW004_001878"
     CDS             543..1442
                     /locus_tag="PW004_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HDL2726843.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            1529..2413
                     /locus_tag="PW004_001879"
     CDS             1529..2413
                     /locus_tag="PW004_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDL2726844.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(2605..2676)
                     /locus_tag="PW004_001880"
     tRNA            complement(2605..2676)
                     /locus_tag="PW004_001880"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2641..2643),aa:Arg,seq:ccg)
     gene            2736..4070
                     /gene="rpoN"
                     /locus_tag="PW004_001881"
     CDS             2736..4070
                     /gene="rpoN"
                     /locus_tag="PW004_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HDL2726845.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            4219..5310
                     /locus_tag="PW004_001882"
     CDS             4219..5310
                     /locus_tag="PW004_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HDL2726846.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            5351..6352
                     /gene="gap"
                     /locus_tag="PW004_001883"
     CDS             5351..6352
                     /gene="gap"
                     /locus_tag="PW004_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDL2726847.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            6462..7652
                     /locus_tag="PW004_001884"
     CDS             6462..7652
                     /locus_tag="PW004_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HDL2726848.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            7756..8511
                     /locus_tag="PW004_001885"
     CDS             7756..8511
                     /locus_tag="PW004_001885"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HDL2726849.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            8627..9925
                     /gene="eno"
                     /locus_tag="PW004_001886"
     CDS             8627..9925
                     /gene="eno"
                     /locus_tag="PW004_001886"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HDL2726850.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            10324..11463
                     /locus_tag="PW004_001887"
     CDS             10324..11463
                     /locus_tag="PW004_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HDL2726851.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT         4014 a         2122 c         2407 g         2961 t
ORIGIN      
        1 aggcagaaag gagtggagaa tgatttttac tggatataag aaaaaacggc agatcgaaaa
       61 ataccgtcac ctgctgaatg gactcatcga aactgattat caaaaaatgt tccgtatcac
      121 gatgaattac gtacatgata aagaagaggc cttagatgtg atgcaagata gttttcataa
      181 agccctgaac tattttgaaa aagaaaaaga gatcgagcat tttagtgcat ggttttatcg
      241 gatgttgatc cgcacggcat tagatagttg gcgcagacaa aaaaggacgc ctgtggactt
      301 atttgaaatt caagatatcc cccaacagtt acctttagaa acttccgttg cagagctcca
      361 tatgatttta gataaaatag acagtccgga aaaagagatt ctgatcctct acttttttga
      421 aggatttcgt ttgaaagaaa ttgcgactat cttggatatg aatgaaaaca cagttaaaac
      481 gaaaatgtac cgctcattga atgctttacg tcaaatccta gaatgaagga gagaagagtg
      541 ccatgtattc ccaaaaagaa aatttaaaaa aactaaagaa aacttataaa gaacaagcaa
      601 ttcccaaagg gcttaaagag gagatccgcc agcgttttat tatcgaagag caatcattta
      661 taaaagcaaa gcggagaaag agacgtctcc aagggttcgg agtatctttg gctatcgtaa
      721 tgattacaac aagtagcctt ctttttaata gtcaagttcg aagctttgca gaagatttac
      781 ccatcttagg atcagtcatt gaattgatct taggcgaacg ctttactgat cgatctgaaa
      841 aaatcgatat tcaggtaccg aagatcaata cacaaaatga gaaagaaaat aaaacaattc
      901 atggattaaa tcaaaaatac tttcgcgaag gacaagcaga ttttgaaaaa gctgaaaaag
      961 aatatggaaa ttttgaaacg gatcattacc aagttttggg agattatcag aaaatcgtag
     1021 acgataaccg gtttctcgtg attgaaaggc agatcaccca aacagcagca gatagccatg
     1081 ttgaaaaaag atacgacaca atcgacaaga aaaacagtgt ccagctctca cttccacttt
     1141 tattcaaaga tgacacctat ctatctgttt tgacaaaaga agtcaaacgg cagatggccg
     1201 aacaagtcaa ggaagatcca tctaagtact attggactga gcaagatatt caagaaggga
     1261 cgatagaaaa accaacactt gttacgccta cacgaagttt ttatttgaac aaagagcatc
     1321 aattagtttt gactttttct caatacgaaa ttgctcctgg ttatatgggt acaccagaat
     1381 ttgtcattcc taagtccgtg acaaaaacga ttttggcatc ggaagattat ttggatcatt
     1441 agacgagaag tagttgcgac tagcagtttc aattttcaag taagtacaag taaataataa
     1501 gaagagaaaa agagaaagaa gagatatagt gaaaagaaat tatttagtta tcagtattat
     1561 tgctttatcc gtgcttttgt ttttcgaaat tacttcggtg gcctccttga tcaccgaagt
     1621 aaaaggacaa acacactctt cggagcattc gtcacaatct ttcaaacaat cagaacaatc
     1681 aaccattaaa agccaatcaa ctgaaacagc taacgtcaaa gaagccattc aagaatcagc
     1741 ggaaaaggta caaagtcagt catcacaatc aacacaagac agtacaaaaa cgagccgaga
     1801 tccggctgtc aaaagcgtcg caatcagctt tgatgatgga ccaggagcga ccacaacacc
     1861 gcagcttctg cgaatcttaa aagaaaaaaa cgtgcatgcc acttttttcg tcttaggaga
     1921 aaatacggcg cagcatcctg aaatcgtgaa gcagacagct gaagcagggc acgagattgg
     1981 caatcacaca tacgatcatc agaatctggc cactctttct gcccagtcga tgacggagga
     2041 agtgacgaaa gcagacactg agatcaaaaa agtcactggc aaaaccccca cttttgttcg
     2101 tccgccctat ggttctgtca cgaatgtagg agcaacgatc atccagcgac cgatcatcga
     2161 atggtctgtt gattcagaag attggaagac aagaaatcct gatttgattt tgcagaagat
     2221 ccaagcaaca gtatacgatg gggcaattat tttattccat gatatctatc ctgaaacgat
     2281 tcgtgccgtt cctcaagtga tcgattatct gaaagagcaa gggtatcgca tcacgacagt
     2341 tggggattta ttagggcatc caacggctgt cgagaactat tatggcagaa acgatcatcg
     2401 gccagttcag taagtgagac aagtatcttt aataaataac acattttaat aagtagttga
     2461 acacataact accttacttg caaaggctta acaatatcta tacaggtaca acaaaaaact
     2521 gtccaccaat tttctttggc ggacagtttt cacttaaaaa taaagaagca actattcgta
     2581 aattatgcta aaatagcagt agaacgcgtc ctgagggaat cgaacccccg tctcaagaac
     2641 cggaatctta cgtgatatcc actacactaa ggacgcaagc actgggataa ttgtaactaa
     2701 acagataaaa aattacaagg ggattggaca aaaaaatgaa atttgagcaa aatttttcac
     2761 aaaaacagca gcagacacag aaactagcca tgacgcaaca gttgcaacag tccatccaag
     2821 ttttacaata taacagtgag gagctgttgg cattcgttga gaatcaagca atggaaaatc
     2881 cattggtgga agttgttgag ccggaatggc agccagatta cataaaagct tcttcatctt
     2941 cttatgaagg agaagagaca aattacctaa atcagattcc tgatacaaaa gggtctttat
     3001 ttgattcatt gatcgaacaa gtccatttga attaccgaga tacttttttg aggaaaatcg
     3061 ttctttatct tgtagaatac atcgatttga acggattttt gacgattagt ttagaagaag
     3121 ccggaaaaga gattggagct acgcctatcc aaatgctcga tgcgttgact ctgattcagc
     3181 aattagaacc agctggagta ggtgcacgta gtttgcaaga atgtctcatg cttcaaacag
     3241 aacgagatga ttatgcgccg gaattagctt atatcgtgtt ggaagaatgt tttgaggaac
     3301 tggtggagcg aaaatggaaa gaaatcgcac aacgtttttc tgttgatcta catgctgttc
     3361 agcagatatt cgactattta caaacgttga gtccaagccc tggccggatt ttcgaccgta
     3421 gcagcgaatt gtacattatt cctgatgtcc gggtattagt ggacgacgat aaaaatgtac
     3481 aagttatctc aaaccgaaaa aatcagccaa atatacgctt tcaagagagt tacttcaaac
     3541 aaatgtcaca acaagcagat aaagaaactg aaaattattt aaaagaacgg aaacaggaat
     3601 tcgaatggtt gaagaaaacg atcctacaac gtggggatac aattttgcgg gttgctcaag
     3661 tcattgtttc ccggcaaaaa gctttcttta tcgacaagga acgtccaatc aagcctctta
     3721 cgttaaagga agttgcaact gagatagatg tgcatgaatc cacggtcagt cgtgcagtca
     3781 acgggaaata tttggaaaca gacttcggtg tatttgaatt aaaaaaattt ttcactacac
     3841 gaattcctac gaatagtaca gaacagacag aagacctttc agcagataca gcgaagaaaa
     3901 agctgcagga attagtcgat caagaagaca aaaacaagcc gttgtctgac cagaaattgg
     3961 ttgaattgct gaaaaaagat gagatagcga tctcaagacg aacagtagca aaatatcgtg
     4021 atttattagg aatcccgagt tcatcaaaaa gaaaacgtta tgataactaa tcagtcaaaa
     4081 gactagaaaa agtatttcac tttttctagt ctttttagtt gaaaaacgag agcaaaactg
     4141 ttagacttac agtcgtgagg ttgagatttc cctttattga tgtgaaattc gaatagttaa
     4201 tttctttttt tatcgtatat gggtcgtttt aagtctacat tggacgtaaa gtgtccaact
     4261 aaggagatcg ctatgctgga tgaaataaaa atgattgaat ctgttgcacc agatattatc
     4321 gaagtgttgc aagaacgcta taaaatttta cgaaatatct attggatgca gccgattgga
     4381 cgtcgaagtt tatcggaaag tatggggatc acagaacgag tattgcgaac agaaacagat
     4441 cttttgaaaa atctgaattt gatcgacacc tcaaaaagcg gtatgacgct gacaactaag
     4501 ggcgaagaag tttatcaaag ccttgaaaac ttcatggatc agcttttagg tacgcatcaa
     4561 accgaacaac agttggcaga atattttggt aaccaacgct gtatcgttgt ttcaggaaac
     4621 agcgaagagc agaccaaagt ggctgatgca ttcggcgaag cgctgagcga agccctagac
     4681 cgactacttc cagaaggcga gaatatcatc gctgtgatgg gaggaactac tatggctgtg
     4741 gtagccgaac aattgtctaa tctggaaaat aagaaacggc ataacctgtt tgtccctgca
     4801 agaggcggga ttggtgaagc gataactgta caggcaaatt ctgtcagtgc aagaatggct
     4861 gctaaagcaa acggtaacca ccgtgcactt tatgttccgg aacaattaag tctggcaact
     4921 tacaattctt tactcaatga gccatctatc caagaagtct taaacttgat tagtgaagcg
     4981 aattgtgtta ttcatagtat tgggcgtgca ttacacatgg cagcacgtcg taagatgact
     5041 gaaaaagaat tagtcatgct gaaacaagca aatgctgttg cggaatcttt tggctacttt
     5101 ttcaatgaaa aaggtgaagt cgtttataag gtccctagaa tcggcataga attgcaagat
     5161 ttggaaaaag tcccgatcat catggcaatc gcgggcggaa aatcaaaagc taaagcaatt
     5221 cgagcttata tgaaaaatgc accaaaacaa acgtggctca tcactgatga agctgctgca
     5281 aatgagattt taaaaggggt aaccctttaa aataaatatt tttttgattt tcataaggag
     5341 gaaatcttaa atgacagtta aagtaggtat taatggtttt ggacgtattg gacgtctagc
     5401 attccgtcgt atccaagatg tagatggaat cgaagtagta gcaatcaacg acttgacaga
     5461 tgctaaaatg ttggcacact tgttaaaata cgacacaact caaggacgtt tcaacggaac
     5521 agttgaagtt catgaaggtt cattcaacgt aaacggaaaa gaagttaaag tattagctaa
     5581 ccgcaaccca gaagaattac catggggcga actaggcgta gatatcgttc tagaatgtac
     5641 tggtttcttc acttctaaat cagcagctga aaaacattta acagctggtg ctaaacgcgt
     5701 tgttatctct gctcctggcg gaaacgatgt tccaacaatc gtttataaca caaaccacga
     5761 aacattaact ggtaaagaaa cagttatctc aggtgcttca tgtactacta actgtttagc
     5821 tcctatggct aaaacattga acgacaaatt tggtgttgtt gaaggattaa tgacaactat
     5881 ccacgcttac acaggtgacc aaatgactct agacggacct catcctaaag gtgacttccg
     5941 ccgtgcacgc gctgctgctg caaacatcgt tcctaactca acaggtgctg ctaaagctat
     6001 cggtttagta attcctgaat tgaacggtaa attagacgga gctgctcaac gtgttcctgt
     6061 accaacaggt tcattaacag aattagtaac agttcttgaa aaagaagtaa ctgttgacga
     6121 aatcaatgca gcaatgaaag aagcttcaaa cgaatcttac ggatacaaca cagacgaaat
     6181 cgtttcttca gatatcgttg gtatgactta cggttcatta tttgatgcta cacaaactaa
     6241 agtaatgaca gttggcgaca aacaattagt taaaactgtt gcttggtacg acaacgaaat
     6301 gtcatacact gcacaattag tacgtacttt agaatacttc gctaacttat aagatagaca
     6361 aacgtacaag tgtaaattat agacatgtac atttaaaagc ggggaagcaa tcgcgcttct
     6421 tccgcttttt ttgataataa gtttattcag gaggaaataa aatggctaag aaaacagtga
     6481 aagatatcga tttaaaagac aaaaaagttc ttgtccgtgt tgacttcaac gttcctttga
     6541 aagacggcgt gatcactgac gacactcgta tcaaagcagc attgccaaca atcaactacg
     6601 ttctagaaca aggcggaaaa gcaatccttt tctctcacct tggacgtgta aaaacagaag
     6661 aagataaaga aggaaaatct ttagctccag ttgcaaaacg tctaggcgaa ttattaggta
     6721 aagaagtaac attcgtacct gaaactcgcg gagaacaatt agaagaagca atccgcaaca
     6781 tgaatgacgg cgacgttgtc gtattcgaaa atacacgatt tgaagatatt gacggtaaaa
     6841 aagaaagcaa aaatgataca gaactaggta aatactgggc ttcattaggc gatgtatttg
     6901 ttaacgatgc tttcggtaca gctcaccgtg cacatgcttc taacgtaggt atcgcttcaa
     6961 ctggtatccc aacagttgct ggattcttga tggaaaaaga aatcaagttc atcggtgaag
     7021 cagtagaaga accaaaacga ccaatgattg caatccttgg tggcgcaaaa gtttctgata
     7081 aaatcggcgt aatcgaaaac ttgattccta aagcagataa aatcttgatc ggtggcggaa
     7141 tgacttacac attctacgaa gcaaaaggaa tcaagatcgg taactctcta gtagaagctg
     7201 ataaagtgga actagcaaaa gaattaatcg aaaaagcagg cgacaaactt gtcttgccaa
     7261 tcgacaatgt ctgtgcacca gaattttcaa atgatgttga aacacaagta atcgaaggag
     7321 acattcctga tggtttgatg gctttagaca tcggcccagc atcagtaaaa ttatttgctg
     7381 atacattaca aggtgcaaaa acagttgttt ggaacggacc tatgggtgta tttgaaatga
     7441 gtaactttgc taaaggtaca atcggtgttt gtgaagcaat cgctaactta gaaggcgcaa
     7501 caacaatcat cggtggcgga gactcagccg cagcagcaga acaattagga tttgctgaca
     7561 aattcacaca catctcaaca ggtggcggcg caagcttgga attgcttgaa ggtaaagaat
     7621 taccaggact agcagcaatc aacgataaat aaacgaccaa gtttgctcaa ttctgacgag
     7681 taatcgaaca aaaggtgaag ggagttttca tgactcccta gccatttttc aataaattaa
     7741 ggagtgtgtt cctcaatgcg taaacctatt atcgcaggta actggaaaat gaacaaaact
     7801 gcatcagaag caaaagaatt tgctgaagca gtaaaaaaca aaatcccttc aaatgacaaa
     7861 gttgattcag tcatcggatc tcctgcatta ttcttacaag aattaatgac atcagctgaa
     7921 ggtacagaac taaaaatctc agcacaaaac tgctactggg aaaactcagg tgcgttcact
     7981 ggtgaaactt caccagcagc tcttgctgat ctaggtgttc attatgtgat catcggacat
     8041 tcagaacgtc gtgaatattt ccatgaaaca gacgaagaca tcaataaaaa agcgaaagct
     8101 atctttgcaa atggtatgac accaatccta tgctgtggcg aatctttaga aacctatgaa
     8161 gcaggcaaaa ctgctgaatg gatcgaagga caaattacag ctggtttagc tgatctttca
     8221 gctgaacagg tgtcaaacat ggttattgct tatgaaccaa tctgggcaat cggtactggt
     8281 aaatcagctg acgcacaaat cgctgacgaa atttgtggag ttgtacgtca aacagttgaa
     8341 aaactttatg gaaaagaagt atcagaagca gttcgtatcc aatacggcgg ttctgtaaaa
     8401 ccagaaaata ttgctgaata catggcaaaa gaaaatgttg acggtgcttt agtcggtggc
     8461 gcaagcttgc aagcagattc attcttgtca ttgttagacg ctgtaaaata attgttgaaa
     8521 atttgcgaaa aatgcaagaa atgtttgcac taatgagcaa aattaactaa aatcgtatat
     8581 gagggaaatc ccttaggaaa aaacaaactc aaaggagaga caaaacatgt caattattac
     8641 tgatgtttac gctcgcgaga tcttagactc acgtggtaac ccaacaatcg aagtagaagt
     8701 atacacagaa agcggagctt ttggccgcgg aatggttcct tcaggtgctt caactggtga
     8761 atacgaagca gttgaattac gtgacggaga caaagctcgt tatggcggta aaggtgtaac
     8821 taaagctgtc gacaacgtaa acaacatcat tgctgaagca atcattggct acgatgttcg
     8881 tgaccaaatg gctatcgaca aagctatgat cgctttagac ggaactccta acaaaggtaa
     8941 attaggtgcg aacgctattt taggtgtttc tatcgctgtt gcacgtgcag ctgctgacta
     9001 cctagaagta cctttatacc actacctagg cggattcaac acaaaagtat tgccaacacc
     9061 aatgatgaac atcatcaacg gcggatcaca tgctgataac tcaatcgact tccaagaatt
     9121 catgatcatg cctgttggcg ctccaacatt caaagaagct ctacgtatgg gtgctgaagt
     9181 attccacgca ttagcttcaa tcttgaaagc tcgcggatta gcaacttctg ttggtgacga
     9241 aggtggattt gctccaaacc ttggttcaaa cgaagaaggt tttgaagtaa tcatcgaagc
     9301 aatcgaaaaa gctggctatg tacctggtaa agacgttgtt cttgctatgg atgctgcttc
     9361 ttcagaattc tacgacaaag aaaaaggtgt ttacgtacta gctgattcag gcgaaggcga
     9421 aaaaacaact gacgaaatga tcaaattcta cgaagaatta gtttctaaat atccaatcat
     9481 ctcaatcgaa gacggattag acgaaaacga ctgggacgga ttcaagaaat taactgacgt
     9541 attaggcgac aaagttcaat tagttggtga cgacttgttc gtaacaaaca ctcaaaaatt
     9601 atctgaaggt atcgaaaaag gaattgctaa ctcaatcctt atcaaagtga accaaatcgg
     9661 tacattaaca gaaactttcg aagctatcga aatggctaaa gaagctggct acacagcagt
     9721 tgtatctcac cgttctggtg aaacagaaga ttcaacaatc tctgatatcg ctgttgcaac
     9781 aaacgctggc caaatcaaaa ctggttctct atcacgtact gaccgtattg ctaaatacaa
     9841 ccaattacta cgtatcgaag accaacttgg tgaagttgca gaatacaaag gtttgaaatc
     9901 tttctacaac ttaaaaaaca aataattctt ttctaatgaa gatacatgat cgactattga
     9961 aagatagtcg agtatgatca cttagttaag taatcgaact ctctacttgc tgaaatatca
    10021 gcaggtagag agtttttttg caactagctt ctgtatatga tgtttacaaa gaatggtaca
    10081 agaaaagatc atccttgaaa aaggaaagct aacaataact agcatcatat aagagctttt
    10141 aaggatagaa cagtagaata ggaatctatg agcaagtcct ctcgtataaa taaaatagga
    10201 gggaagtagc agcatatttt tcataaagtg gctctctttc ttcattttta ctttggttgc
    10261 tcctatttga aaattcgagc acactcaaat acaataaaaa gaaatggcaa gggagaggat
    10321 tagatgaaaa aagaagaaaa aatcgcgatt ttacaagaga tcatccgaat taaatcagtg
    10381 aatggaaacg aaggtgaagt agcagcctat ttgaataagc ttttggcaaa gcatgacata
    10441 acaggagaga ttgtttctta cagagatggt agagataatt tgattgctcg ttaccaaaag
    10501 ggccaatcag gaaaagtgtt aggcctatct gggcatatgg atgtcgtggc ggctggtgat
    10561 gaatcgtctt ggacctatgc accctttgca gcagaaatcc atgggaaccg tttgtacgga
    10621 cgtggtgcta cagatatgaa gagtggcttg gcagcaatgg tgattgccat gatcgaactg
    10681 aaagagtcag gaaaaccatt taacggaaca gtcaaattat tagccactgt cggagaagaa
    10741 gtgggtgaac taggtgggga acagctcacg aaagcaggct atgtagatga cttagatgcc
    10801 ttgatcatcg gagagccaac caattacagt ttgatgtata cgcacatggg atcaatcaac
    10861 tatacagtca cctcgcacgg aaaagaagcg catagctcta tgccagacca aggttataat
    10921 gcaatcaatc acctaaatga attcattacg aaagcaaatg ctgaaatgaa tcacctcgct
    10981 gaaacaatcg agaatcctgt cctaggaaaa acaatccata atgttacatt gatcagtggc
    11041 gggaatcaag tcaacagcat cccaagccat gcgcagctcc aagggaacat tcgatcgatt
    11101 ccagaatacc caaatgataa aatcattgca ttgctgcaat caatcgtcaa tgaattgaat
    11161 caagagacag actatcattt ggaattgatg atcgattaca acaaaatacc tgtcaaagct
    11221 gatccagatt ctcctttgat tcattgcatt cagcaacaat tcagccagcc attaccttta
    11281 gttggagcgg cagcaacaac ggatgcagcc gaatttacga aagctaacca ttccttcgac
    11341 tttgtcgtct ttgggcctgg tgtcgttact ttgcctcatc aagtagatga gtatgttgaa
    11401 atcgataatt atttggatat gatcgaaaaa tatcaaggaa ttattttatc ctacttagcg
    11461 taagattttt ttaataaatg aaaaaacaga gccagattag catt
//
LOCUS       DANKKG010000085        11270 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000085 DANKKG010000000
VERSION     DANKKG010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.085"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..646
                     /locus_tag="PW004_001888"
     CDS             <1..646
                     /locus_tag="PW004_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="small subunit of terminase"
                     /protein_id="HDL2726852.1"
                     /translation="TNQGGAPIGNQNAKGNKGNSRASPPVGNKNALKTGEYETIFFDT
                     LTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERLQQLRK
                     IKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAIKQMNE
                     LYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKENE"
     gene            639..2054
                     /locus_tag="PW004_001889"
     CDS             639..2054
                     /locus_tag="PW004_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDL2726853.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPESMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTKVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            2066..3595
                     /locus_tag="PW004_001890"
     CDS             2066..3595
                     /locus_tag="PW004_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDL2726854.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEDMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKAPTAKAEYVTKNLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            3513..4565
                     /locus_tag="PW004_001891"
     CDS             3513..4565
                     /locus_tag="PW004_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HDL2726855.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            4567..4884
                     /locus_tag="PW004_001892"
     CDS             4567..4884
                     /locus_tag="PW004_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726856.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            5011..5634
                     /locus_tag="PW004_001893"
     CDS             5011..5634
                     /locus_tag="PW004_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HDL2726857.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            5649..6539
                     /locus_tag="PW004_001894"
     CDS             5649..6539
                     /locus_tag="PW004_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HDL2726858.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            6562..6777
                     /locus_tag="PW004_001895"
     CDS             6562..6777
                     /locus_tag="PW004_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726859.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            6789..7130
                     /locus_tag="PW004_001896"
     CDS             6789..7130
                     /locus_tag="PW004_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726860.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            7130..7501
                     /locus_tag="PW004_001897"
     CDS             7130..7501
                     /locus_tag="PW004_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726861.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            7494..7889
                     /locus_tag="PW004_001898"
     CDS             7494..7889
                     /locus_tag="PW004_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDL2726862.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGEVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            7891..8268
                     /locus_tag="PW004_001899"
     CDS             7891..8268
                     /locus_tag="PW004_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726863.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNNPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            8269..8877
                     /locus_tag="PW004_001900"
     CDS             8269..8877
                     /locus_tag="PW004_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2726864.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            8877..9218
                     /locus_tag="PW004_001901"
     CDS             8877..9218
                     /locus_tag="PW004_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726865.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            9433..>11270
                     /locus_tag="PW004_001902"
     CDS             9433..>11270
                     /locus_tag="PW004_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HDL2726866.1"
                     /translation="MNNDDLILKMILDESGFTAGMNNAVKKLNSFDETIERKSRNSGS
                     SLGSIWKIFAGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNS
                     EIMKNIGKNGLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFG
                     GSTDQVNEAVIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLS
                     QGKISVEEFQNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIE
                     GLNKALVDSDLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALK
                     FMMPFLVPAATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANPFVL
                     IIGAVVGAIAVFGYFMATNEELRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEM
                     ASNAVEGVKDAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKW
                     FSDTWQSIKDSAREKWNGIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKN
                     VFEILKNVILAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIW"
BASE COUNT         4113 a         1549 c         2298 g         3310 t
ORIGIN      
        1 tactaatcaa ggtggcgctc ctattggtaa tcaaaatgct aaaggtaaca aaggaaatag
       61 ccgagcttct ccgccagtgg gtaataaaaa cgctttgaaa acaggtgagt atgaaacaat
      121 attttttgac acgttaaccg ataatgagaa ggacatctat tctagtttga atgatgatcc
      181 ttcttttgct ttgtccgaag aaatacgact tcttaaaata agacaacttc gaatgatgaa
      241 aagaatccaa caagctgaag ctggactaaa tgatgaagaa gtcgaacgat tgcagcagct
      301 aagaaagatt aaaactccga ttgaaaaaaa tggtaaaaag ctagaaatca agcgtgaggt
      361 tatgcaagat gtgcagatta gcagaaaaaa acatcgcaaa attgatgata ttctttcaat
      421 tgaagattca ttgactcgga ttagcaacca gttagctaaa gccatcaagc aaatgaatga
      481 actttatatg aatgaataca gaactgattt aattaaagct cagactgata agatccaagc
      541 tgagacaaat gaaattggcg gaaataattc aggtgaagaa atagaagaat ggaaacaggc
      601 agttttaaat gccgcaaaca aacgggcggt gaaagaaaat gaataatgaa tttattcctt
      661 ttgctgatat tggttctgcc attgattatt actatgataa accagtagct ttttgccaag
      721 atattttgca tttgaatcct gatgaatggc aagaaaatgt tttaaatgat ttagctgaat
      781 tttcaaaggt ttctgttcgt tctggtcaag gagttggaaa aacagcatta gaagcaggag
      841 caatactttg gttcttaacg tgtcgaccct acgctaaagt aatagcaaca gctccgacaa
      901 tgaagcaact ttacgatgta ctttgggcag aggtagctaa atggttaaat gatagcttga
      961 tcaaaaactt actgaagtgg acaaagacca aaatttatat ggttggtgat tcagagcgtt
     1021 ggtttgctac ggctagaaca gcgactaaac cagaaagtat gcaaggtttt cacgaggacc
     1081 atatgttgat tgtggtagat gaagcttctg gtgtgtctga tccaattatg gaagctattc
     1141 ttggtacgct atcaggtttt gataataagc tgttgatgtg tggaaacccc aataatattg
     1201 aaggtgtctt ttatgattcc cataattcag accgtgataa atacagagtt cataaagtat
     1261 caagctatga tagtaaacgt acaaacaaag acaatataga aatgattctt aaaaaatatg
     1321 gaaaagaaag tgatgttgct cgtgtccgta tttttggaga atttccgaaa ggtgcgttgg
     1381 attcatttat cagtcttgaa acagttgaat tggctacaga aaaacaaatt agtgattctt
     1441 tagtcaataa aacaaaggtt gctcatattg gtgtcgacgt agctcgatat ggtgatgatt
     1501 ctacgattct ctttcctaga attgctacca gggcattgga gtatgagaag tattcaaaac
     1561 gtagcaccat ggaaacaaca ggatatgtca tcaacatggc caagaatcta atgagtcaat
     1621 atccgagtat tgataaagtg atgattaaag tcgatgacac tggtgtcgga ggtggtgtaa
     1681 ccgaccgcct agaagaactt atagaagaca aacattatcc ttttgaggtg tttggagtga
     1741 ataacggttc aacatcagaa gacgattttt acgataattt aggtactcaa ctatggggaa
     1801 acatcaagga aatgttagaa gaaaatatga cagcaaatct taacggagaa cagcctgtta
     1861 ttgaattgcc ttctgatagt tcgttaatca aagaattaag tactcgcaaa ttcaaaatga
     1921 caagtagaag tcgtatacgt ttagaaagta aagatgatat gaaaaagcga aatattggta
     1981 gtcccgatat tgctgacgca ctggctttag cgttttatga gccaccaagt cactatcaat
     2041 ttattcaatt ttaggaggtg agcttttgat tagtacagta ttatctttag agagatataa
     2101 gaagctacgt gttaaatatg caacacaaat tgaagatgga atgtttgatc cgaatggttt
     2161 tatagaagat atgaaaccat tttttgctga tcgcgaaaga aaatatctag cttatactag
     2221 cgaaaagaat gaaatagata atagaccaaa gcctaacact gatattgtaa aagttaataa
     2281 taaacttcat gctggtatgt attcaattgt cgtagaccaa gcagtcaatc attttactgg
     2341 tatacctatc aaatgggatt acgatgtatc ggaacaaaaa agaacactca ttcaaagatt
     2401 aaaagataaa ttcttaaaaa atgatataga acttccgaca gttccagaag cttttaacaa
     2461 attaacaagc aatcttgatt ccatgagatt cgcaatgctt gattctgaaa ctgcaacttt
     2521 tcaaggagct tgtggagtag ctttccgatt gctagagcct gtggaagaag atgatggttg
     2581 gaagttaaga gcaagtaata ttgaaccttg gagagcagaa agatatggaa atgccggaat
     2641 ctatatcaaa gaaaagtatg actcttacca gaaaaaattt tttcaagaaa tgaaagttat
     2701 gacaagaaat aaaattctga catatgcttg ctatggtgat ttgaattttg ttacaagcgg
     2761 aacgttcaaa aaaattgatg agaccgataa ccctttgggg acgattacct tggcagaatt
     2821 taaaaataat acgaatcgtt attgtgattt tgaagtagct gaggaaattg gagatgcaat
     2881 tgatcgtgct ttgtctgatc aacaaaacga aatcgaacaa tttaagcttg cgtacatgct
     2941 tgttactggc acaacaatga gcaaaggcac tgctaaagaa atgatgaatc agctagggat
     3001 tatcaactta aaagccccta ctgcaaaagc tgaatatgtc acaaaaaatt tagcaaaaga
     3061 tttcaatgaa tatcatatgg atctattgaa aaaacagttt tacaccattt gtaaagcaat
     3121 cgattttaac gatgaggtgt ttaaatcaaa cagttctgga gaagctcgca agtggcaaat
     3181 cattagccta gaagcgaaaa caaatactaa agagcaatat tttagagaag gattgaaaga
     3241 atgcgcagaa acaattgctg cttttcttaa gtttcatgac aaagtagaaa ttgagcctga
     3301 aaaaattatt tttactttct ctagatcttt gccaactgat ttaagttatt tagctgaagc
     3361 tttacctaaa cttgcaccat acgtatccaa acgaactatt cagagccaaa ttccatttgt
     3421 aacagatgtt gattatgaaa atgaaatgat ggaattggaa agtggaagtg cttatccaga
     3481 tagtgaatac aattttggcg gaggtggcaa tagtgacgat agaaacgaag tattggacca
     3541 aacgtcgaga actggaggat caagcaaggc tcaaacaaga aaatcagaca cttaaaaaat
     3601 taactagtgt atttcctgaa gcactgaaag agatacaagc aaaactatta tcacaagctg
     3661 acttacacaa tatcacttat ccagaaatga tggagtttta cagtacaagt aatcagaaaa
     3721 aatatcgtga atatgtggaa aaaaattata agtcattaaa aatgtatgat gcaaaataca
     3781 aagagtttat cgatgaattt tttccaccat ttgactatgc aaaagtcaat cgcttattac
     3841 aaatacgatc agatgtattt aaaattcttg cagaatatgc gatggatgca gatgtgaatc
     3901 aatatttttc tgatcgctta gaggaaattc ttcaaagaac atattcttct aatgctaatg
     3961 tttttgttca acttttaaac gttgatatac caaattattt accagaaaat gaaattaaac
     4021 attatttgaa ctatccatgg tcaggaaaga cattttcaag aagactttgg ggaaatattt
     4081 catcgcttga acagaaactc tctaatgcta ttgtaaaaag tgttgctagt ggagaaggcg
     4141 ttatacacgc attaaacacc atgagattag actcagaaat ttgtgacatg tttaagttag
     4201 aagaatcaaa gtataacaaa gcgatagaaa atctcgttcg aacggagtat gcaaaatttg
     4261 cacaagatgg tattgaaaaa tcatatttag aaacaggtat tgaggaatac aacgtattga
     4321 ctgcaaaaga tgagagagtt tgccggattt gtggaggaaa ggcaagtaag aatccctata
     4381 aactgaaaga tgctgtcata ggtgaaaatc gagcaccttt ccatagtcgt tgtagatgta
     4441 cggatgttcc taatttacca aaattaggaa aggatattga tgaagaatat gaccgtttat
     4501 ttggcgattt attagatgag tttgcacatg attcttttgg aattaatttg aaacggagga
     4561 agtagaatga aagatttttt tgaagcagta ctaacaatta atgtaaatgc tgatattgca
     4621 gaagcctaca aaacagctat tgagtctgag aaccatccta atggcttgag agaccattgg
     4681 aatggcaatt atgcctacgt ggttattggc gatcaaactg ttaattatca agataatact
     4741 ccagttgata agaataccgt taatttaacg attcaattat tatctcattc attaccaaat
     4801 ttaaaagaaa cagttgattg gtatgaaaag atgggatgta ttgttgttag aactgactac
     4861 aaagaaggaa agtctagtaa ttaggctttt ttattttgtc cgaaatgacg ttaaactagc
     4921 gcaatactgg gcttggttga atggtggggc gcaactatta aaactcaaag caatgcgggg
     4981 cgtgaaaacg aatcgtgggg cgaaaggaga atgagcatga aacacaaatc attaatgcca
     5041 atgaatttgc aatactttgc cgaaggtgac gatcaaaagt tttcttttga tgacttcaaa
     5101 tcttttgtgg aatccaatga agaagctcaa aaatttattc agtcacaatc tcaaagtgtt
     5161 gccgacaaac aattggaagc ttggaaacaa aataacttag ataaaattaa acaggatacc
     5221 atcaaggaat atgaggaatc taagaaaaac aagtcacctg agcaaattca actggaaaaa
     5281 ctacaagctg aatttgaagc agaaaaagcg ttgcgtgtga caagtgataa taaagcattt
     5341 gttgcagaac aaattgctgg attagaacta gatggagagt taaaagagtc tatttctcaa
     5401 tttatgctaa ataatcttgt tagttcggat acagatttca ctaagaatgc tgttgaaggt
     5461 tttacaagtg ttttgaatgc aattaatgag aaacatgcag atgcattaaa agaactacaa
     5521 atgaagtctg catttggtgg aactcaacaa tcgaataacc aggttcagca gaacaatgaa
     5581 acattaacaa atccagaaga acaattagga caaattcttc aacaatttaa ctaggagagt
     5641 gaaaaattat gaaaaaaaca tctttaaata atttagagta tttggatatt tcaccagcga
     5701 ttaatgctat gcaagtacca aatacacctt ttttaagcta tttacttggt gctggaaaaa
     5761 cagagccagc aaactcgaca gaaattaaat ggcgtgaata tgatatcaac aacgatgatt
     5821 cttctgaaaa acttgagggc ggagaatatc cagatgctga atcaggtcga acttggttta
     5881 acaactatac tgaaattttt agaaaatcaa cctctgtatc tggtacatta gatgctatta
     5941 atgtgaatgg agtcggaaat gaattaacta atcaagtagc actacgtggt atggaaatga
     6001 aaattgattt gaaccgaaaa ttgattactg gtgtaaaagc tgatgaaaat ggttctaaag
     6061 gtcgtcgaat gaatgggatt ttgaacttga tcaattcagc aaataaggca gaaacagcca
     6121 ctgcgggtgc agtaacaaga aaagatatcg atactttatt taaattgatg tatgaaaaag
     6181 gttatatggg agaaaaacta tgcttgattt ctccagatat gcaggagtta atgactgatg
     6241 agttagatgg aaaatcaaca aaaattgttc agttcgggga aagagtaact tttggattgc
     6301 aaattggaaa tatcgtgtct aattacggta caggtattgc tctactagaa ccatcattgc
     6361 caaaaggaac aattgccgca atcgatacta attatgtgaa actacgtcca ttacgtgaat
     6421 ggagagcaga agaacttgca aaaacaacgg attctagacg tatcggtctt gtaggtgaat
     6481 actctcttga atacaatgct tcaaactctg gggcaatttt aaatttaaag tctgaataaa
     6541 agggagttag tactcccttt tttgatagga ggaattacag tggtaaaaaa agatgaaact
     6601 aaaaaagatg aagtcgtgaa atatagagta ggtaaaacta aaaattttgt tgggtttgtt
     6661 catcctaaaa ctcgtagatt tatcacagca gattcaaata acgaatttat catttctata
     6721 gatgataaag aagcaattgc aattttggaa gatgcaattg atgttaatga aatttaggaa
     6781 gtgatctgat ggatgaatcg ctaaaaacgg aaatcattga gtctacaaaa gaagattttc
     6841 cagatttgag tgaagaacgc ataactaatt tattagaaat aattttgcta gaaattgaat
     6901 catacaacac ttgtaaaaat gatatttcat gggaaaagtt aaaaagcgtg attagtgaag
     6961 tgttgtataa aataataaaa aatgaatcag aaaaaacagt atcttcagtt agacgtggcg
     7021 atacaacgat tagttatgct tcaacaacaa atgatgttag tgagttactt ctaggctatg
     7081 gtgatttgat acgaagagtt attggttgtg gaggattgga gtttttttaa tgaatgaagc
     7141 agatatttta gaaactactt atgaagatag ttgtattatt gaaagactga cggacattga
     7201 agattctaat acaaatatta ctattcaaga ttacaaaaaa gtatatgata atcctatttc
     7261 ttgcgctctt tcacaaggtc aaatcgatgg actagcagtc atagaagatg gagagatggt
     7321 aaatgtttca actgacacat ataaattatt tgttcatcct aagattaaac taaaaaaagg
     7381 agatcgaata acaataactc aaaaggccag tggcttaatt ttttctctat ttgctactaa
     7441 gcctttttac tatcctagtc attgtgaagt gaatttgata ggaagtgaga aaaatgggta
     7501 atctcaaata tgaatcaaat gcagaaaaga tcattgagaa ttttaaaaat atgactgtaa
     7561 ttgctcaaaa agaaggaata tccttcgtta acgattcaat gaataaagtc gttagtttag
     7621 ctaaaccatt aactcctgta aaatcaggta atttaaggcg aggatatcgt gtagtaaaag
     7681 ctagaaaact atcaagcggt cgaattgttg gagaagttat aaacaatgaa cattatttta
     7741 aatatgtaga agaaggtcat aggactaaaa atggtggctt tgtcaaaggg aaattcatgt
     7801 taactcgtgc aacgaatctt gcaaatatgt cttatattcc tcgaagattt aaacaaatgg
     7861 caataaaaat cgttaagaaa ggaaagtaac atgaaagatg aaatcattgc tgcaatcagt
     7921 agcaaattaa aagaaatcta tcctgatggg acaatatatc ttgattcagt tatgcagtca
     7981 actaaagatt tttatttcgt tttatcagta atggaatctg gaactgaaaa tgtaggaatt
     8041 gatattcaaa atgtttcttt cttaattgat attgcattga ttgataataa ccctaataga
     8101 aatttaatta atgaattagt ctcacgttgt gggacttttt ttaatacgat tacaatcgat
     8161 gagcaaatat tatttccaaa agcctattta cctgatgaag cagatggtgt tcaacatatt
     8221 cgttttacat tagaatttcc acaatatatt gaatggagtg aaagataaat gggagaaaaa
     8281 agaagtaaaa ccggaattat ttctgtcgaa aaacctactt ggtttccttt ggaagatgag
     8341 acagggaatt ttccggtata tggtactgcg ttgccaatgg gaacagcagt aagtatcaaa
     8401 ccaacagcta attatgaaac aacacaagat tatggggatt cagtggttca agatcaattt
     8461 acagcttttg gtggtgcaga agttgaactg gaagcaaatg gatatactca taaagtttta
     8521 tctacaatta ctggagggaa aattgttaaa ggtggtgcct tgcgttctgg agaagatatt
     8581 gcgcaagatg gtgcgtttgc atatagacgt aaaaaatcga atgggaaata ccgttatacg
     8641 gtcttttata aaggacagtt tgctctagat tcagatgaaa catcaacgat tgaaggaagt
     8701 aaagtaagtt ttactcatcc aacttggaag ggttcatttg tagatgtacc aggacttgga
     8761 tatatgtatt cagttgatga agatgatgaa ggcgtggata aagcaatgat cggaaattgg
     8821 tttacaaaag tagcaattcc aattgaagaa gcagaacttt caggaggtac agaataatgt
     8881 caaaatatca aacaacgatt aaattaacga aaaaagacga agaaggtaaa tatgagcaag
     8941 tacaatttaa atctgctgaa tttttaccag gaacagttgt agaagatgct gcaggagtta
     9001 tggaagaaat gcaaacagcg actgataagc aatcagttaa aaaagcttta agtcgtgctt
     9061 attcatttat tgcagatact ttatttgagg gacaatttac tggtgaagat tattgcaaag
     9121 gaattgatgc tcgtgagatt gcctcattga caggaaaatt attgaagtct gttactgcag
     9181 gttttgatga aacttataca gagacgaaaa aaaagtaagt gaggcgctca agtcaccttc
     9241 atttaagtat tcgattactt accgagaatt agatataaag acgcaattac tcgaagcagg
     9301 ttggacgtta ccagaaattg aacgtactga cttggatgag ttaatgcgtc tttttgcttt
     9361 tagagatgcg gtaaaagagc atgaagatgt tgagtactat gataatttca ctcaatttta
     9421 ggaggtgata ctttgaataa cgatgaccta attctgaaga tgatactaga tgaatctggt
     9481 tttactgccg gtatgaataa tgctgtcaaa aagttgaact cttttgatga aacaatcgaa
     9541 agaaaaagta gaaatagtgg cagttcatta ggtagtattt ggaaaatatt tgctggaagt
     9601 tttctagcta gcggagtaac tagaatcgta ggagctggct ttgatctaat caagggttcc
     9661 ataagtggag cggttgatcg agtagatacg atgaataacg ctctacgaaa tttccaaaat
     9721 atgggattta gtaattcaga aattatgaag aatatcggaa agaatgggct tttatctcaa
     9781 ggtattcaag gacttcctac tgctttgaat gatgcgataa gtcatgttca acttcttgct
     9841 tcttctacag gtgatttaac tcgttcaact caaatattta aagctttaaa tgatggaatt
     9901 cttggtttcg gtggttcaac tgatcaagtt aatgaagctg ttattcaatt atctcaaagt
     9961 ttctcaaacg gaaaagtaga cgcacaaact tggaactcaa tgatcaacgc tcaacttgga
    10021 cctactcttt ctgctattgc taaaaagatg ggaattacaa tgggagatct gaaagaaggt
    10081 ttatctcaag gtaagatttc tgttgaagaa ttccaaaatc aattaataga aatggatacc
    10141 aaaggtggcg gaggacttaa atcattaagt caaatcgcta aagattctac taagggaatt
    10201 aaaacctcta tacaaaatgc aaaaacagct gttacacgtg gtgttggtga ggtaatagaa
    10261 ggattaaata aagcattagt agattcagat ttaggtggat ttaagggaat tattgataaa
    10321 gtgggcagtt caatggaatc gttcttaaaa gtaattgctg caaatattcc tacagcagta
    10381 tcatttttaa gtaatttatt taacgaaatt caaaaatttg gttctgcttt gaaattcatg
    10441 atgccctttc ttgttcctgc agctaccgct tttggagcgt tcatgtttca acttaaaggt
    10501 ataccagcaa taataaaaag cttcaataat tttaagaatg ccataattgg cgttggaaat
    10561 tcattaaaga ttatgggggc aatagctgct gctaatccat ttgttttaat tattggtgca
    10621 gtcgttggtg caatagctgt tttcggttac tttatggcaa ccaatgaaga gcttagaaac
    10681 aaggttatat ctatttggaa tgatgtgaaa gattccgtaa ttggtgtatt aaaaaatata
    10741 aaggactggg gaattgatac ttggaattct gctaaagaaa tggcatcaaa tgcagttgag
    10801 ggtgtcaaag atgcttggtc aggaacaaag gaatggtttt ctaacacctg gaaaggtatt
    10861 aaggatgggg ccacaggttt atttgataaa acagtagaga cttcaagaaa tgcagtcgat
    10921 agtgtaaaaa atgcatggtc taatatgaag aaatggtttt ctgatacttg gcaaagtata
    10981 aaagattcag ccagagaaaa atggaatgga atcaaaggtt ctattatgtc agttgctggt
    11041 ccattgatta caggtataaa gaatgcattt ttacatgtta ctttttatct agagacctta
    11101 tggaataatt tagttgagat tggtaaaaac gtctttgaga ttttaaaaaa tgttatactt
    11161 gcgccggttt tgtttattac ctccatgatt tctggtggat gggaagaaac aaaaaataat
    11221 atgattgctg tttggaataa tattaaagaa agtgccataa atatttggga
//
LOCUS       DANKKG010000086        11116 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000086 DANKKG010000000
VERSION     DANKKG010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11116
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.086"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            72..592
                     /locus_tag="PW004_001903"
                     /pseudo
     CDS             72..592
                     /locus_tag="PW004_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(494..1210)
                     /locus_tag="PW004_001904"
     CDS             complement(494..1210)
                     /locus_tag="PW004_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HDL2726867.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(1260..1469)
                     /locus_tag="PW004_001905"
     CDS             complement(1260..1469)
                     /locus_tag="PW004_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726868.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            1746..3019
                     /locus_tag="PW004_001906"
                     /pseudo
     CDS             1746..3019
                     /locus_tag="PW004_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(3126..4157)
                     /locus_tag="PW004_001907"
     CDS             complement(3126..4157)
                     /locus_tag="PW004_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2726869.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(4164..4994)
                     /locus_tag="PW004_001908"
     CDS             complement(4164..4994)
                     /locus_tag="PW004_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2726870.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(4991..5806)
                     /locus_tag="PW004_001909"
     CDS             complement(4991..5806)
                     /locus_tag="PW004_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL2726871.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(5803..6627)
                     /locus_tag="PW004_001910"
     CDS             complement(5803..6627)
                     /locus_tag="PW004_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HDL2726872.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(6617..7717)
                     /locus_tag="PW004_001911"
     CDS             complement(6617..7717)
                     /locus_tag="PW004_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL2726873.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            7895..8680
                     /locus_tag="PW004_001912"
     CDS             7895..8680
                     /locus_tag="PW004_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HDL2726874.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            8713..9096
                     /locus_tag="PW004_001913"
     CDS             8713..9096
                     /locus_tag="PW004_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDL2726875.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            9172..9303
                     /locus_tag="PW004_001914"
     CDS             9172..9303
                     /locus_tag="PW004_001914"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL2726876.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            9272..9508
                     /locus_tag="PW004_001915"
     CDS             9272..9508
                     /locus_tag="PW004_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL2726877.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(9586..10557)
                     /locus_tag="PW004_001916"
     CDS             complement(9586..10557)
                     /locus_tag="PW004_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDL2726878.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
BASE COUNT         3323 a         2571 c         2120 g         3102 t
ORIGIN      
        1 aactcaaaaa gagactcact tttggtaaaa tttagtcgac caaaacatca ccctaccaaa
       61 ggagtcgctc tatggataat catactagaa aattactcgg attaacagat aaacatttat
      121 tttttgatga agaatggctg attgaaaaag aatataaagg cgttcaagcg cagtttatta
      181 aagggagatt ggatgactca ccttcccatt gtgaacactg tggatcgatt cgtattatcc
      241 gcaacggttc ctatacgaca cgtactcaga tgttgaaagt caaagaaaag ttgactattt
      301 tagaattaaa acgtacccgt tttctatgct atgattgtgg gcagaccttt tctgcgaaaa
      361 cggacttagt tgatgaacat catcaactaa caaaggaact gaaacaggcg attctcatag
      421 aactgtacga aaatcaatca cgtaatctga aaatcagaag aagtaccgtc ggctcaagcg
      481 atattggaaa ctcttactca aagattccac gacattggag ccgctcaaac ggcattatca
      541 tcgactgttt aaacgaccaa ttagtcaaac agaaatcgtc gatgaaccgt tgtgcacaat
      601 ggtgtcctga acgtacagcg acaccttcca tatccagagc agtcgccaca tcacggggat
      661 ggatgccatc caaattaaaa gcaaccacgc cagtatgatg ttcaggatct tgcggtccgt
      721 aaatggtgat accgccgatc gcttgtaatt taggtaaaac gtaggcaacc agttcttgct
      781 cataacgatg aatattctcc ataccaatct cattcaagta atcgattgct cctgcaatgt
      841 tcggagtacc agcttcaaat ttccatggca gctctttcca tgaactatcg taaagattga
      901 caaaatctat catttcaccg ccgaattcga ctggctccat ttgttctaac aattgacgtt
      961 tgccatataa tacaccgatt cctgtcggcc cgcacatttt atgcccgcta aaggcgaaaa
     1021 aatctgcatc caacgcttgt acatcaactg tcatatgcgg tgctgcttgg gcaccgactg
     1081 gactatcctc aactacaaca gcctgttcaa ccctcagccg tttcaagcac aacaaataac
     1141 cttctggatc tggtttgccg acctgaagat catcgatcgt cacaaaggaa tcaatttttt
     1201 ccttaatcat cggatgtgct tcaaaaaaat agtctagctc ccgcagttca gaattgagac
     1261 tactccgatt ttccagtgct gctgatccgc tgctcaaaat gttcagcatc cactggtctc
     1321 ccaaccgctt gacttatccc ttcataaaag gacagttctg ctgccgtccc aacaccttgt
     1381 tgatagtctg caaaaggaat tttatagccg aactgctcca gtaaataggc ttcagtaaat
     1441 agtgatgaat actctcgtaa aacatccatt cactgtcaat catcgtccca tcaaaatcaa
     1501 aaataagtgc tttggtcatc ttctttcccc cttacccaaa acaagcaaaa gctgtgtcct
     1561 gctctctcta cagtatcagc ataaaaactg ccgtctgaaa agtcaaaggt gaaagaaatg
     1621 tggcgctata atttctagga ctgatagtaa atcattctat cagtcctttt gagcataaaa
     1681 aaacaaaaca tacagaagct ccgttagaat tgaatcacca caacacaatc aaaggagcgt
     1741 tctgtatgcc tgatcttcat cttatcaaac tattactagg aatcaaagat ggaaacattc
     1801 aaatcactca tgtcgagaag aaaaaaatac attcaatcaa cagtatcgtt gtttctgcca
     1861 cgctcattaa aacgattcag ccctgtcctc agtgtaaaca ggtgaatcaa catgggctga
     1921 tcgttaagaa cggaaagaaa aaatcgctgg tccttctcaa taagtgtgcg aatcaacgga
     1981 cttatcttgc tttggcaaag caacggtacc tctgtcgtgg ctgtcacacc tattttacgg
     2041 cgaataccta cattgttgaa aaaaactgtt ttatcgccaa acaagtacat tataaaatat
     2101 tggaggaatt gactgaaaaa caagcaatga cgacgatttc taaacattgc ggcgtttctt
     2161 ggtcaaccgt ttcgcgaaca ctcgcttctc tgctccccat gacaaacgtg aaaataaatt
     2221 ggttgcctcg ctgttcattg gtagatgaat ttcgttcatt gaaaaatcaa gtcggtccct
     2281 attctttttc ttgtatggat ggcgatactg ggaaattact ggatattctt ccttcacgta
     2341 agaagaaaga tctcatttct tattttatgc aatttgaacg acgtgcacgt ttaaacgtaa
     2401 aaatactcgt aacagatatg aacgcctctt atgctttgct gataaaagag tgctttcctc
     2461 atgccaaact cgttgtggat cgctttcaca tcgtcaagca cctcattcgc agttttaagg
     2521 atatttgact atgagtcatg aaaagcttcg accgaaacga tccaatacaa gccaaacact
     2581 atcgacaagt gaaggcttta tctcgacttc tgatcaaacg acaggacacg cttgtttatg
     2641 ataaatggac aaaatggcgt aattttggtt gggcctattt aaccgaaagt gaagtcgtag
     2701 aacgtctact ttctacttct gatgaactac gaatagctta tgcttactat caagaaattc
     2761 ttcaagcttt ttatgacaaa gaagcggaca tcttctttca gcttgtaaaa acaatgccca
     2821 agtcggttcc aagagaactg catcatataa aaaaagcgtt tataaactat gaatccggca
     2881 ttcgtttagc cttagagttg ccttactcaa atggcaaaat agaaaatctg catacccaca
     2941 tcaaagcatt gaaacgaata gcttacgggt taggatgaga accagaattt tcttaatcaa
     3001 tcatctgatc acttattaaa cataaaaaaa catctaaagc agaaaatcaa ttctacctta
     3061 gatgttctta tatcactcta tcagtcctat ttgacaaaga gccagaaatg tttgccgact
     3121 atacactagc tttgttgtgc aaaatgctga gtatcaattt ccacagggat tgctagaata
     3181 tctttcagct gaatgtataa ggcatcgcta attttctctt cccgatcgtt caaccgatcc
     3241 actaaaattt ctttgccctc cagcgtttga aaaacatacc gttgaatgtt gcctaaaata
     3301 aatgattcct tccatagaac cggaatctta accccctgag gaaccggttc aaattgaatc
     3361 agctccggtc ttaaataata aatgcagcga ttgcccttag cggaaaactc gtgggtatac
     3421 tgattcagct cataccattc gaaacgatta tagttgccga taaattccgc aacaaatcgg
     3481 ctctgaggat tctgatacaa ttccttcgag gtggaaacct gagccacccg cccagcttcc
     3541 atcacaaaaa tccgatcact gattcgcatc gcctcttctt ggtcatgagt cacaaaaatc
     3601 atcgtcatcc ctagcgattg ctggatagcg cgcaaatccc gctgcagctg ttttctaatt
     3661 cgggcatcca aagcacttaa tggctcatcc agcagcagga ctttcggttc agtgaccaat
     3721 gagcgagcca gtgccacccg ttgtcgttga ccgccagaaa gaaactggac atttttatct
     3781 gcctgctccg tcatatccac caatgatagc atctcaaaaa ccttttcctg aatcacctca
     3841 ggactcagct tctttacctt caaaccaaag gctacatttt caaaaacagt catagttgga
     3901 aacaatgcat actgttgaaa aacaaagccg atattgcgat ccttcgtagg cttatcggca
     3961 atattctcat tgtccagcac aatctcaccg ctctttggtt gttccaatcc cgtgatacag
     4021 cgcagcaacg tagactttcc gcacccgctg ggacctagta aagtaaccag ctcacccttt
     4081 tcaatctgaa tttcatcaat agctaaaatc tgtttttcat caaaaaacag attgagatca
     4141 tgtatttcta aaaacatttt ccgtcacaac tcctttaagg gcttcttttt cgactgcttc
     4201 tcttccctcg tctgcttaag ggacagctgt tgcttttgtc gataagtcag cataaaggta
     4261 gttaaagcaa ttcccaccat aaacagcaaa tacaaaacca tcacagcgct agccaaatgg
     4321 ccgttttcgt tcatccgccg cagcatatag atgcgtaccg tctgaaatcg accgccaatc
     4381 aacaggttcg tcagcatgta ctccccaaaa accgaagaaa aaatcatcag agaaactagg
     4441 gtcacaccga tacggatgtt tggaaacagc acctggataa taatcgtaga catcggggcg
     4501 cccaaggttt ctgctgcatc cactaattcc ttcagattaa ttaagcgcat ctggttgtta
     4561 agactaagat agacaatcgg caacgccgta ataaacaatg cacctaccaa cactacaaac
     4621 attgggatac cagtttcagc atacgttcga agcaaagccg ttaccaaaat cactcctggt
     4681 aaagcatacg gcagcaggag cagcttttcc agccaacgat tcaaacgagg gaaatacaaa
     4741 tgaatccaaa taatggtggg aatcatcaac aacagaatcg tcaccaagac cacacctgct
     4801 aataaaagtg agcgacccac cgcagcaata aattccctgt ccgtcagcag ctgttggaac
     4861 cattgcaagg taaaatcact aggtaaaagc gattttgacc atttagtgga gctggcatac
     4921 aaaatcgttc caataatcgg cagcaagtaa tacaggacaa agcagaacaa aatcggtttt
     4981 attaactttt tcacgtctgc accaccttgc ctttcttttg aataatcaac attgccgtta
     5041 ccagcaggac acctaaagcg actgccaacg cactgcctaa attaggcttg gcgaacacat
     5101 ctcccgacac taaagccgca attcgaatcg ttaaaagatt tacattactg ctggtcaggg
     5161 catacgctgt ttcataggtc cccatgccat tagcaaataa gatgacaaag gtgctggcaa
     5221 tcgatggtcg caaaaaaggc aaagccacct tgaaccaagc atacgctgat gaagcgccca
     5281 gcaattgtgc ggactccagc cattcctttt tcacttcctt catgctggga tacaaaaaca
     5341 agacccctaa cggaatttgg aaataggtat acgtgatggt caatccccac caagaataca
     5401 aattgaagtc cgctagaaat ggcaaaatca ttttcagcac acctacattc cccaacaaaa
     5461 taataaaaga gaatgccagc ggaatcccag caaaattagc tgccaaattg gaaacaaagg
     5521 tgattttctc ctgcaattta gtcgatagat tcattaagca taaacataaa ataaacgtcc
     5581 cgattaaccc aatcacactg gagctgacag caatcagtac gctgttaaca aaggcctgtg
     5641 tgtaatattt gttgctgaaa atatcactgt aattggccaa tgtccactgg ttagttaaat
     5701 cactgtgcaa gctgcacaga accataaaaa gcagcggcag cagtaaaaat gcacagaaga
     5761 taaaggaggc aggaaccaga accaagcgtt ttttcatctc tttcatcccc tcatcaattc
     5821 ttgaatgtcc tgcattttct ctgacggtgc aattcctaat aaccagcaaa acaatggagc
     5881 cgccagcagc tgtggtacag tttgttcctt cagcttttgg cgtttatttg aaatcaaata
     5941 aaaaggaact tcacgatgtg cagccaagct gccgccatgc agtccgtgcg catccatccc
     6001 gtgatccgca gtcaccacga cttgataacc tttttgcagc cattcaggaa ggtaaatagc
     6061 caataatctg tccgcctgat taaccgctgc tgcatattca tgagaatcgg cggtaaattt
     6121 gtgaccgaca tcatcaatat tcatcgaatg tacgaccaaa aagtctggct gatactgatt
     6181 cactaaataa tgggcatccg caaataaatg cgaatcaggg taagtgtcct cactataaaa
     6241 gatgccatgc tgaatcagtc gctcctcctg atgctggatg cgatcaacca aacgattata
     6301 gggggcttga ttgtacaact cgctgtacca gtggtaagcg gcggctcctg tttttccgcc
     6361 agcttcctta actaattcaa acagcgacca ctctttggat cgctgagcat ggtaattact
     6421 atagatgcca ttttcatagg ctggaacgcc ggtcatcagt acttcgtata acggacgtga
     6481 attgctgggc agctcacttt ttaccttcaa taagcttgct tgctggtgct caactaaatg
     6541 attcatgaac ccaagctggg cgacggctgt gtcgtagcga cagccgtcta gtataactac
     6601 aatcagtttg tcaagtctac tttgcatagg tcagtacctc cgcctgccaa agctctggaa
     6661 gctctacaga ggtcttgttc caagcttcat tgtcttcaac tggcaccgcc gcttcataag
     6721 catcctccgg cagtaatttt tcagctactt ctttaggcag ctctaccttg cgaataggtc
     6781 gcgcatagcc ctttgccaaa ttgatttgcc cttcatcaga taaaatatat tcccgtgcta
     6841 gctttgccgc attgggatga ggggcatttt tattgattaa ggttgtatac ccgctaatga
     6901 tggagccgtc tgttggcaca gttacctcaa aacgctcgga gtcaatttgg tcacgataat
     6961 ttagtccatt gaagtcccaa acaactgcca catcgatttc acctttttca agatttgcta
     7021 cgctggcatc aacggtggac aaacggcctt tttctgccat ttttttgtag taattcagac
     7081 ctggctgaat atccttttcg tcacctccgt tggcaatcgc cgcagccaat acagcaaatt
     7141 gtgcttggtt ggcaatcgtc acatccccca cgctaacttg atagtcaccc tcttgcagct
     7201 ccttccagct agtcggtgct tgcttcacat tctctttatc ggtgataaag gaaatggttc
     7261 ccgtatagct taacaaccaa tccccatcat catcctttgc ccaatccgga atttcctccc
     7321 aatagttggt cttataaggt aaggttaact ctttgctttc agccaacgga ccgaagctga
     7381 taccgacatc accaatatcg gcggttccat tttttccttc tgattcaaac ttagccaatt
     7441 cttctgcact ggacatatcc gtatccacgt gacttaagcc gtattctttt tcaaggtcgt
     7501 tccaagtgcc tacccaattt gcccaagtat ccggcattcc caagctggca atctctcctt
     7561 cttccttggc tttttctata atcgtatcta aagagtcgtc tgccgcagcc gattgcttcg
     7621 ttgcttcacc tgagccgcag cctgtcagca ggatacttac tgttgctgca caaaataaac
     7681 cgatagtcat gttacgaatt ttttcattca ttttcattca ttcttctccc ctttcgataa
     7741 ttccattcta acaaagcttt gtaatgagag aatcagtttc ttgaaaaggt ttcatgaaca
     7801 atgtaaaggt tgtatatggt ttgtaaattt cgacgaattc ccgttaacct tctaaagctt
     7861 ttgcggtatg atagtagaaa aaaggaggct ccctatgcag tattatgatc agcatctcca
     7921 cacgtatttt tcctttgatt cagaggaaaa atttgaaaac tatttagcct atcagccgga
     7981 gtttttcgtt tctacggatc actttgattt aaacaatcca tctaccggaa aagatgacat
     8041 tccggattat ccagcctata taaacaaact ggactcttta aaagaaacct attccaccaa
     8101 atttctgcga ggtatcgaga ttggcgttgt tccaggacaa gaagagcgga tcgtggatta
     8161 cctcgctcag catccttatg atttaaagct ggtaagtatt catcaaaatg gccgctttga
     8221 ttatatggac gatgtggttt taaccaaaga tcccattcaa actaccaagg aatattttga
     8281 tcagatgttg caggtcttga cggattttcc agaaggagat attttgactc actttgatta
     8341 tggattacgc cgcttcacct tcacagcgga agaattcgcg gagcattttg aggctcagct
     8401 gctaacgatt ttcaaaaagg ttgttgaacg agaattggcg atggagttaa atgccaagag
     8461 cttcttgaaa tacggcaacg aagaccttta ccgttatgcc gttcctttgt atcaaagtgt
     8521 cggcggaaag ctctttacct taggctcgga tgctcatgtt gccgaagatt atcagctggg
     8581 ctttaaaaaa atgagtcagc tgttggcaga ctgtcaggta gaaaagttgc tagtcattca
     8641 aggaaaagaa cgccgcctga cgccgctgcc gattttgtga ttcctatata atagaagaaa
     8701 gtggtgaaaa aaatgagcaa cctgtgggac tacaatcaag aggcacccat ccattattta
     8761 attgcgcgac attgggatgc gttgaaaatt gaggcggttt gccgatcctt gttagcagcc
     8821 gttccaaaac aacaactgga aaattttttg gtggctgaca gcttgcagcg ggaaaaagtt
     8881 caggcttatt ttgcagcctt taaagatcag cctttggaat atttgcatgc gcagtttcat
     8941 cttttttatc aagtagccgc cccagatgac tacaacgatt tacgaggaca gctgcaattg
     9001 acctttcaag cggatgaaac cgcctatacc gttttattgg gaatggctcg ccttggtgat
     9061 caggcgaagg tcgagtggcg gattttcgat atttgatggc aggatcaagg cgtttcagtt
     9121 gcaaaccttc ctctctttca attagaaaaa ccgcagcagg aagaagtttg tgtgctggtc
     9181 accgatgctg gtgaaattcg tctccgctta tttcctgacc aagctcccct ggctgtagcc
     9241 aactggcgct ccctagctga gcaaggcgtt tatgacaaca cacctttcgg ctcgtgtcat
     9301 taaggaattt gttattcaag gtggcgcttt ggatggttct ggtgatgaag ctgtttccag
     9361 ctatgagggc tttttcgctg atgaagtgca tcgcggactt tatcatttta atggcgcctt
     9421 ggccttaggg gaccacggcc cccacaccaa cggcaaccag ttttttattg tgcaaaacac
     9481 aaaggcgcag gctgacctgc taatgtaaag caagcatact tggagcttgg aggactaccg
     9541 gagctggatg gtcgttatac tgtttttgga caggtatacc aagggttaga aatcgtagca
     9601 gcgatcgcag ctcagccgac aaacagtgaa gatgtaccga ttgagccagt gttcattcga
     9661 caaattaact tcgaaaaaac atcgtaaaaa gacactcact gacttcagca aaagctctat
     9721 tcttgagctg gctgcagcta gtgagtgtct ttcatttatc cataagataa agcttggatt
     9781 caataaataa ataaatgttt tggttaaagc tgtgaccagc aaatgaggaa ccgtgcgagc
     9841 cagttgatct gccaatgcaa acatttcttt aggtgaaggc gctggcgtgt ctgccaacgg
     9901 atactccagt cccaatgctt ggtaaccgtg cataccatag cagtggtacg gtagtaattc
     9961 taacggtacg aaccgatcaa gatctgccac aaaacaagca atcgtcagca gctcggtcag
    10021 gttcatattg atacctggaa ccacaggaac tcgaattcgc acttcgcagt taggatactc
    10081 aattaaaaag cgacgaatat tcttcaaaat ttgttggtta tctaagccgg tccattgttt
    10141 gtggagcttg ctggtaaaca tttttacatc cacaaacaat gtatccacat agggagctac
    10201 cttttggatt tcattataag caccacaaaa gctggtttcc agtacggtat tgattccaat
    10261 gtatttcgat ttttgcaaaa tttctttagc aaaatccgcc tgaaccagtg cctcgcctcc
    10321 gctaatggtc actccgccat ccgagtgaaa gtaaaaaaca gcatccttct caatctcatc
    10381 cattacctct tcagcggtca tggtctggcc aaatcctttt tcaatctgct tgctttgtga
    10441 ttcgggaatc gaacaccagg cacagcgtaa tgggcagccc tttaaataaa caacggtccg
    10501 taaaccagca ccatcatgta gagaaccctg ttctattctt aatactgttc cttccatata
    10561 gatttttgaa ttacacacta gtttaaacta aaacacacaa ggaaaaactt accatttaat
    10621 cataaaatta gctcatataa atatactagg atgtaaaata gactggtcaa aatctggtca
    10681 aaaaagagga ctaatacagg tagtcctctt ttttgttatt ttaatattct aaatcatttt
    10741 ttattggttt attcccttat tcgatgtaga ctcaaatcac tatatcacta gatatcaaac
    10801 ggcgtcatac ggaatcaaaa acatcaaatt tgacctgtta aatggtcatt gaataacatt
    10861 caaaatcaat aaaacggagt cagacacgga gtcaaaaacg ctgagagtaa aaaatatata
    10921 cagccttgta ggtaaagtac cacagggctg tatatttttt atttcagatt accttaaatg
    10981 attccattta ctttttatct cattttttcc tactagttag tatcaaatac tctcctattt
    11041 tctcaataaa ctatttttgg atacatggta tctgaaacag ctaaaacctt gtcattacag
    11101 caaaaaatca cgttat
//
LOCUS       DANKKG010000087        11003 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000087 DANKKG010000000
VERSION     DANKKG010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.087"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(<1..187)
                     /locus_tag="PW004_001917"
     CDS             complement(<1..187)
                     /locus_tag="PW004_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small subunit of terminase"
                     /protein_id="HDL2726879.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELISNVTNGKSN"
     gene            complement(346..576)
                     /locus_tag="PW004_001918"
     CDS             complement(346..576)
                     /locus_tag="PW004_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726880.1"
                     /translation="MRNYWYVSLTNEYPRTIDDCSVRVVRSVQIKGKYSIVEMGREAE
                     PCEIDACKLVYCGHGFYDEPNIQNNINKNLRD"
     gene            complement(1323..1790)
                     /locus_tag="PW004_001919"
     CDS             complement(1323..1790)
                     /locus_tag="PW004_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HDL2726881.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQHR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            complement(1865..2305)
                     /locus_tag="PW004_001920"
     CDS             complement(1865..2305)
                     /locus_tag="PW004_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL2726882.1"
                     /translation="MSVIPQLEGEEAIVNILENIDIKQTRKNARRLLKRYRNLERLVG
                     PVKIDFSVMTVTKNLKFTIDSQNEEIIEAISTRDSVIEALTRLSRIHFQVLYYSYCFP
                     NKMSMYQIGEKLGYSDRTIERMKAVALVEFAEAYKSGELISRTK"
     gene            complement(2335..2628)
                     /locus_tag="PW004_001921"
     CDS             complement(2335..2628)
                     /locus_tag="PW004_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726883.1"
                     /translation="MKLKDGFYSSGHGIGGLMLDMPTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVVRLVDMEVIKS"
     gene            complement(2659..2853)
                     /locus_tag="PW004_001922"
     CDS             complement(2659..2853)
                     /locus_tag="PW004_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726884.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNEFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            complement(2959..3411)
                     /locus_tag="PW004_001923"
     CDS             complement(2959..3411)
                     /locus_tag="PW004_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726885.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            complement(3437..3898)
                     /locus_tag="PW004_001924"
     CDS             complement(3437..3898)
                     /locus_tag="PW004_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL2726886.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            complement(4099..4950)
                     /locus_tag="PW004_001925"
     CDS             complement(4099..4950)
                     /locus_tag="PW004_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL2726887.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            complement(4965..5765)
                     /locus_tag="PW004_001926"
     CDS             complement(4965..5765)
                     /locus_tag="PW004_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDL2726888.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADDIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            complement(5808..6698)
                     /locus_tag="PW004_001927"
     CDS             complement(5808..6698)
                     /locus_tag="PW004_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HDL2726889.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            complement(6700..7641)
                     /locus_tag="PW004_001928"
     CDS             complement(6700..7641)
                     /locus_tag="PW004_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HDL2726890.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            complement(7874..8209)
                     /locus_tag="PW004_001929"
     CDS             complement(7874..8209)
                     /locus_tag="PW004_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726891.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            complement(8365..8538)
                     /locus_tag="PW004_001930"
     CDS             complement(8365..8538)
                     /locus_tag="PW004_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726892.1"
                     /translation="MKKTDTLFIGFILGLLVIVAHQSIIGGSLFAALMVLINLLDSKE
                     RSNYGTRRSAKNR"
     gene            complement(8535..8723)
                     /locus_tag="PW004_001931"
     CDS             complement(8535..8723)
                     /locus_tag="PW004_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726893.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            8801..9025
                     /locus_tag="PW004_001932"
     CDS             8801..9025
                     /locus_tag="PW004_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HDL2726894.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            complement(9022..9177)
                     /locus_tag="PW004_001933"
     CDS             complement(9022..9177)
                     /locus_tag="PW004_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726895.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGKKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(9317..9529)
                     /locus_tag="PW004_001934"
     CDS             complement(9317..9529)
                     /locus_tag="PW004_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726896.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            complement(9532..9744)
                     /locus_tag="PW004_001935"
     CDS             complement(9532..9744)
                     /locus_tag="PW004_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2726897.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            10019..10429
                     /locus_tag="PW004_001936"
     CDS             10019..10429
                     /locus_tag="PW004_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2726898.1"
                     /translation="MLLDRIKILLKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKI
                     QKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPL
                     DDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
     gene            10442..10894
                     /locus_tag="PW004_001937"
     CDS             10442..10894
                     /locus_tag="PW004_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HDL2726899.1"
                     /translation="MHLNKYWILEEIEKLFVEFGSDAINPYKIIKYSDIDLIETDLGN
                     KTLGQTIHNKRCYVILINQNLSPSMKRFVLWHEIAHVRLHKGICTAAFRANNLSCMIY
                     GIEAEANMFAIEMLTRTIDEQDLSDMSQFQIIEYLGLPHNLEHLVLQH"
BASE COUNT         3094 a         2290 c         1731 g         3888 t
ORIGIN      
        1 cgttactttt gccattggta acgttactaa tcaattcagc gctccatttg tcttgcgatt
       61 tccattttct aacctgagaa tctgaaacat ttaattcaga tgcaatttcc tttaactgct
      121 tttctccgtt ggattctaac caaattcttt tggcttcatc acgtcttgga tcacgttgtc
      181 ttgccattca atacacacca cctcacattc tgtttaggtt gagttttgtt ttctaatttt
      241 caatatcttt tagcattaaa tcagcttcaa tcaatatctt taaatcggaa actttgtcta
      301 acttgatttg tcctgattgg agatttttaa gccatttctt gcattctaat ccctcaaatt
      361 cttgttaata ttattttgaa tgtttggttc atcatagaaa ccatgaccgc aataaacgag
      421 cttgcacgca tcaatctcac atggttctgc ttctctcccc atttcgacaa tagagtactt
      481 ccctttgatt tgtacagaac gcacaacacg cactgaacaa tcatcaatgg ttcgaggata
      541 ttcattagtt agcgatacat accagtagtt tctcattatg tagcctcctt tatgtaaaga
      601 aaaagaccac tcaaagagtg atctaatatg taatgcacag gcagggacgt ttccgatcct
      661 gtgcttgaga catttgacga ttcatttgta ccgaatccca aatctcaatc taacctaacc
      721 agttatgtaa tagcaaccta cacgatgcac aaaacgcgta cgaaattgcg cacccctata
      781 tttttaaacc gccgatgcct cggttgccaa agtcactggc aaggaatcga accttgcatg
      841 gttgccgaag cattgaccta gcacacatgc ttagcgtcta ccctttccgc cacagtgaca
      901 ctataaaatt attcttggct gctactattt tttattttgc ccatttttaa atccaatcat
      961 atatacatta agacaaagcg caaaaattga aattattaat ggaatcattt ctcttcactt
     1021 acctttagtt atcgtgtgaa taattaaaaa aacaatagac agcaacggat gatagataat
     1081 aagaacaatt tagaaggagt tgaaattcac atccttattc ttaatatttc cgttgctgcc
     1141 tatcgaagct taattaaacg atgagggaga tttcctccct tacattttat tttgtcgatc
     1201 ctgtttccta atttttcgac actatcataa tacaacgttg aataggtaag tgattggtat
     1261 aaaaaaggta taaaatggaa accaaattgg taataaaagg gtataaaaag tgtaaaaact
     1321 ggctacttga aagcaaccag ttctaacgat gaagcaaatt ggatgataat tctgtttgat
     1381 tctactttaa ccgattcttc gctagtatta ttcctttgag cagttacata aatgggcaga
     1441 ccattgatat aacgatcata gaatatcttc ttgcgccttt cagtcacatc aggcttatgc
     1501 ggatgctgta tcgctgaata gcctcgaaca aacaatttat gcagatactc aaattcttcc
     1561 tgtgcttctt ctttatcgat cagcattctt tctgcttcaa atatatgatc agctgtagaa
     1621 ggtggaacca aggaataaga tgctgtcact tttggttctc gatgttgccc tactctacat
     1681 ctagctgata gatatgctga aagaaacaca gcgacattat gttttgtgcg ttccatatca
     1741 acatctttcg cgcttggtgt ctcatatttc tttacatcga aaagtaccat cctctgattc
     1801 ccccaattat gatataatac ttatgtcgga aatattattc atagtcggag gaatccgact
     1861 ttttttattt tgtacgtgaa atgagttctc ctgatttata cgcctcagca aattcaacca
     1921 aagctactgc cttcattctc tcgatagttc tatcggaata tcctaatttt tctcctattt
     1981 gatacattga catcttatta ggaaaacaat aactataata aagtacctgg aaatggattc
     2041 tacttagccg tgttaatgct tcgataaccg aatctcgagt acttatcgct tcaatgattt
     2101 cttcgttttg actgtcaatt gtgaatttca aatttttggt aacagtcatc acggaaaagt
     2161 ctattttcac tggtccgacc aaacgttcta aatttctgta tctttttagt aatcgtctag
     2221 catttttcct tgtttgctta atatctatat tctctaaaat gttcacaatt gcctcttctc
     2281 cttccagttg tggaataacg ctcattgttg taggatagtt gtaaaaaagc aaaattaaga
     2341 ttttataact tccatgtcca ccaatctcac cactgctaaa ttctctttgc ttttcgctaa
     2401 ccacttatca cattccatcg tgttttcaat gcgaatgatt gctgagtgat tatagacgtg
     2461 ttctacatat ccacgaaatg gatagatgaa tccttctgct tcgcagcgaa ccatgtcacc
     2521 gactttgaat tttggtttct tacgtgtttt aggattcttt gtcggcatat ctagcattaa
     2581 accgccgatg ccgtggccgc tggagtaaaa tccgtctttt agtttcattc cgcttcctcc
     2641 tctttagctg tcaattactt actcgcaagt cactaaaatg gctacttcat cgataaagtt
     2701 tccgtcttcg tcttccattc cttgctgaat gctaatgtcg gtaatctgct taaaaaattc
     2761 gttgtcatag ccttgtgcgt ttatcactac ttcgacgtct ccgaaaacct ctttgagact
     2821 attaatttta tttactactt ccgatgcgtc catgactaca cccctttcca gttaaaaatc
     2881 tctgtcgtta atagactact gttcaaagta ttcatcttca ctcatccttc tgcctcctct
     2941 ttagttgtca ttagtggact aaatactacc tgtttgaaaa ccataatgtg cagtttcaac
     3001 gccgtcataa tcacttattt cccactcagc cccatcgggt atatcaacga ctttaagact
     3061 tgcatatggt ccactggcct cacttcctag tttttcgaca acagcaacga ggtccttatc
     3121 acctctgttt gaatcacttg aaaaactgga atcatcagca attccgtaca gtcggtcaat
     3181 ttcgctataa attcctgtaa ctttgtcgat tttcgggtct tttttgaaat agtagataaa
     3241 atccatcgaa cctagctttt ggcctgtgta tctcgtgtat tcatcatctg agtcatagct
     3301 aaagttattg atgtatggga atacttcaat attttttctt ttaaaaattt ccatttttgc
     3361 ttcatggctc aacccaaagc caccaaaaca tttatttaat acaatcttca ttacttttcc
     3421 tcctttgttt cctccgttag ctgaattttt tcataaacac aagccaatgt gttttgcttc
     3481 gtttattacc aaacaatggc tgttctccaa tagcctctaa cacatcagaa agtggtatct
     3541 gttcctcgtt ccacttaaaa attaatgttc ctgaaggtcg taaaactcgc atacactctg
     3601 caaaaccttg cctgatgtct tctggccaaa gttcgtctaa ttttccatat tttttggcaa
     3661 gccatgagtt ttcgccgaca tgtcttaagt gtggcgggtc aaatacaacc atatcgaaca
     3721 catcatcttc aaaaggcata tcgcgaaagt ctgcgaccac atctggatca acattgatca
     3781 catgacctgt tgataattct tcatactgct gacgacaatc cataaatgtt acgtcagaat
     3841 tgttcttatc aaaccagaac aatctactac cgcaacatgc atctaatatt tttggcaatt
     3901 actctacctc caataactct ggattctcgt agacgttacc taaaacagtg agaaatcttt
     3961 ctgtatcccg ataaataatt agcttactta tcttccgaac cattcgactc tcttgttgag
     4021 ataattcgtc catgtcacga tcacctataa tttgcaacat tatctgacga cagattcttt
     4081 tttcttgttt agtcaaattc atgcgatcgc tcctttgact ggttttatgc gcttgtctgc
     4141 tgtttgagaa aataccatcg taaatccatc agagttgaca aacattcttg atattgttct
     4201 cgtaccgtaa gcttttttca gttcttcacc aagcaagttc gttgtgataa tcgttgctag
     4261 gttctgtcgt gcatccaaga acgaatttag cgtgtttatg ccaaacgctc gactatctga
     4321 tgcatctttt cctagttctg aaccgatatc atcaatgatg actaaatccg ctgttttgat
     4381 atctgcaatc aacgatccct caatcgtttt tctgagttca ggattgttgt acgaaaattt
     4441 gatttgatcc agcatttcct gtaacccgat aaataagatt tttttatcgt agtttgatcg
     4501 cttcaagact tcccaagccg ctgccatcgc taggtgactt ttacctgttc cttgttttcc
     4561 agttaaaaca aggtgacttg gatttcctaa caagactgaa ttaacaaagc gtttggttac
     4621 ttcaaccgct tgtcttgttt cttgatcgac aatttgataa ttctgcaatg tgcaatcaaa
     4681 caacgcttga ttaggcacaa cagaaccacc tttgaaaaaa ttgattgctc ttgctttcag
     4741 gctctcgttg taaattcgtt ctgtttgtaa gtcttcctga actcgcaatg ctttataccc
     4801 acattgcata caggttggct tacaacgttc tgagccatca gggttcttcg tacgccatcc
     4861 atacaaaggc tgtccgcatt caggacattc gccacgttgg acaagcactt tctgtatcag
     4921 cttttccatg atttccccaa cagtttccat gcctacgcct cctctcaaat aggcaactca
     4981 tctgtgctaa atttctcgta ttcaagcgtt ttagtttgtt ttgttttata atcacgattt
     5041 gcttgcgctg ccattgtgtc gtatttttct cgcaattttt tagcagataa aatattcgat
     5101 gcccaaaaca cattgtgctg actccattca atcattcccc gtacctggtt ttcggtccgc
     5161 ttgtcgatct cgatcatttt ccgaatgtca tctgcccaac tttgcagatt cggctttttg
     5221 atttcctgat tctgacaaat ctgtttgaat aactcctccg cgagaatgta gtaaactgag
     5281 tcggtgtcgt aaacacgctt tttgcgtggt tgcgacgatg atgttttatt attcttttca
     5341 ttcttataat tcttttcatt cttgtttgtg tgcacttgtt gttcacttgt tgtgcgtttg
     5401 atgttcattt gttgttcact gctttggtac aaagaccagt tttttattga tataacgctg
     5461 tatttcgtag ttgatttgat gttcaacatt ccgttttttt caaattgttt tagccatctc
     5521 catacagaac cgctgttcac ttgatgttca cgtttcacgc cttcgttcat ctcagatgtg
     5581 atagcatcgc gccctgtgac gaattctccg ctgttcactt gtatctcttt tccattaaaa
     5641 agtatcttgc gattctcatg actcgctttc atcaaacaca agttccataa tttatacata
     5701 taaggattgg tccaaacgaa tgaattcatc acttttcgat gtaatttgac atatcctgtg
     5761 ttcattcgta tttctcctct agaaaagcaa gggagaaagc tccctcatta tttgtttaac
     5821 ggcggattag atgcatcaaa taatccagtt tgtacatctt cattttcttc agatataacc
     5881 tctgcttctt ttctttcagg aatatcttct tcaacttctg tttcagcaat aatgctgccg
     5941 tcttcttgaa ccctttggac tctctcatcc gatgtggtgg cttcttgcat ttcgatggac
     6001 aagattcccc atttagaaag aagatttctc aaaacagttt ttcgtgccat tgcattgtaa
     6061 tcagatgccc acacaccact taactttgtc ttatcgcgat ctttattgtt agcaatccga
     6121 tgagcttcga tttcttgttt ggtccaatag acagtttttt tgaatccatt cagtaactcg
     6181 aaatagccaa catatccaat gacttcatca gacgttctac catttggatc aaactcaaac
     6241 tcttctgtca gtcggttcca gctttttagt tctccttcgt aaacttcgat cacatttaat
     6301 gctttgtatt tacctgatcg ttgggctaat tggatatatc ctttatagcc aagcatgaat
     6361 tgagctttct tttcccattt tcctgtttgc ttgtttttac tattgaatgg aactaaatat
     6421 gcataaccta aattcttatc tagcccaaga tttaatgttg cagcagttaa cgcaccactc
     6481 atgatagaca tcggttcact atctgcaaga taactgtcat tagatacaag agtcataaca
     6541 ttcgacataa aagcattagc attgtcatga agcacctctt cgaacttctt tctcattgtt
     6601 ggtgtattca tcaaagcttt aagtcctaat tgacctggtg caacttgttt ctgtggcttt
     6661 gctgccaatt gattttttaa cgattcattt gttgccatat tatttgatct ccctttcggt
     6721 tagccttctt gattcagtaa cgttataaat ctcttcatca tttgcgacat ctggatattt
     6781 ctctgctagt ttcttcgagt tcatacgtct cgtacggaca aatttccaac tgatgatgtt
     6841 tttttgagtg ataccgatac tggcttcacg tttacctagc tcgctgataa tctcgttgtc
     6901 tacttgacgg atagctgatt caatttcttt cttagtccgt ttgagttcgt ttttctgttc
     6961 aactagttca tcgaaatgaa ttggtaacgt cgtttgaacg tcttcaacat ctgcatactt
     7021 ctctttcaag aaatcagctg tcgcttgact accatcaata attggctcga tacctccaag
     7081 aacgtttgtt tcccaaaact ctactaattg ttcagtgatt gtatcgatca gctcttgatc
     7141 tcgttcaatc cgcttccaga taaatttttg accaccgatt aagacagcga tgtaacaata
     7201 gtctttgttt agaacgttca tgtaatgttg aacttgacag agatagctaa gcggtacctc
     7261 ttcgccttcc cattctttac cgagaaattg attggctgtt ttgcattcca gaatggcatt
     7321 ttctcccact acttcccgat caatatttgc tcttaaaaat ggatgtaatg gatgttcaaa
     7381 tacttggttt cttctgcgta cttttttgcc tgtacgttcc tgaaactctt tagcaaccac
     7441 ttcttctaaa acattacccc aataagcggg ctcacttcct gattcttcaa gtactacttg
     7501 tcctgttttt tctagccata gttgataagg agatttccat ttattcaatc ctaaaattgt
     7561 tccaacatcc gaacctccga tgcctttctt acggtcttca agccattctt gatggctcat
     7621 ttctaaggta gatttactca tcttcatcct cctcatcaat tggtgcttca tatggtggtt
     7681 tagcataatc aggatcagtt aaatagttgt caagatttgc taactcgttc atgtttaacc
     7741 tcttttctgc ttgctaacac gcaaagcaca agcatggtaa ttgcgaataa caagccaaag
     7801 attacataac ttttcgctac aatcactaac gtgaaaaata aaataatgag tagatcaatt
     7861 gttttttcat tcatcattga aattccccct tgagaatacg agtcatcaca tcagctaaat
     7921 cttccttgtt attaataaca aaagtatgat ttgaaggttg attttcttct tcatttttcg
     7981 cttgttcgta agcatccaca gcaattctac gcataatatg tgctgacaat gggagatttt
     8041 catctaaatg ttcttcttgc atagcaagac aaagtaaaat ttctgttgtc tcaccataaa
     8101 cgagactttt aagttcccct tctttttggg ctgtacaaat aatagaaaca ccttgctttt
     8161 ggcattcacg tttcaattct tcgataagat tttcaatttt cttattcatg tgatataatt
     8221 ctcctagttt tataaatttg tatgtgactc tttgctggct ggcagagtca cttttttatt
     8281 tgttgccatg ctttttgctt ttcgatatgt tgcttgctta aaataatagg gcggctattt
     8341 ccccaccgat tatcagcaat cactttaccg atttttagcg cttcttctcg tgccatagtt
     8401 gctcctttct tttgaatcaa gcagattgat taaaaccatc aatgctgcga acaagcttcc
     8461 cccgataata ctttggtgcg ctacaatcac taataacccc aaaatgaatc ctataaaaag
     8521 tgtgtctgtc ttcttcataa tctaatctcc ctatttttta tttctagcat tctcaaatcc
     8581 tcaagttcag aagcaattag ttcagcttgc ctatctgata gctcatcggc ttttctaagc
     8641 gctgcacggt catcttgtaa ttgtttcctg cgttgtttaa tcaaaccgag aatttgatgt
     8701 tcttgttgca aggtatatgc catatcactt ctcctttcgt ttattgaatt tctccatttc
     8761 tatttgataa aatatttcta tagaaatgga ggtgaaaact atgggaaaaa atcaacatgt
     8821 tgtacctaat tccaatggtg gttggaatgt caaaggtgct ggaaattcta aagcgactgc
     8881 acatactaat acaaaatcag aagcagtaaa aatcgctaga gagatttcta aaaatcaagg
     8941 atctgagcta tttattcatg gcagagatgg aaaaattcaa agccgtgata gtcacggaaa
     9001 cgatccacac ccaccaaaag gttaatcata tttaggagtt aatcgaatac gataaccgtc
     9061 agcaggagtt gcatcggtca atgtaacttc tgctactttt ttcccttctt ctgtttcaat
     9121 aattaatctt gtgtaattgt tctcattcaa aatccctatt tttgattctt ccttcatgtc
     9181 atccctcctt cgtctctcgc cgtcaccacc tccaagaaga ggagttgcca aaatgaaaaa
     9241 acgtggactt tggcaaactt gaaaggagag cagcaactcc tcttttcaga agtggtgaat
     9301 ttagattttt tagctattaa caaacagctc taactctaaa tcgattttta gaacgccatg
     9361 ttctaaactg ttcatataaa tcgacatcaa ttgttatttc tttggaagta actgcgttat
     9421 acccctcact aaaacgtgat tttttaaatt ccttcattct tctttgataa gtactttcag
     9481 aatacttata tttctcttta aactccgctt tgctcaaacg ttgtaccatt tttacgcctc
     9541 ctgttctgat ggctgatcat tagaatctac ttctaacttg atatccaaaa gttcttcgat
     9601 ttgttttaaa cgttccttag gttttctttt ttcgatgaag atatctcgta aataagtaat
     9661 tgatataccc attcgtcgag ctaactctct ttgactccaa cctttcttta ctaaagcaat
     9721 taatacttgc tgttttaaag tcatgcttca taccccctta tcgaatattt tctaatattt
     9781 ttgtctaata agtttgacaa aaactgcaaa tatgctatta tgtaagcata caaaatacag
     9841 cgcacaaaaa gcgagcttac tgatgtctgg ggagactatt agtttattgc ttttagtctt
     9901 tgtatgccgt aaatatcttg cttacaaaac tagtataact gcaaattctc tatttgtaaa
     9961 tagataattt gcagaaaaat actttttttg tcggtatttt gaaaggaatg catatcctat
    10021 gctattagac cgtataaaaa tcttattaaa agaacgttca atgacgttgg cagaacttga
    10081 aagaaagtta aatttcggaa atggaagcat gtctagatgg aataaaagta ctccatctgg
    10141 agataaaata caaaaagttg cagattactt taatgtatca actgattatc ttttaggacg
    10201 tactgataaa cgccattatt atgatttaac agaaaaagat aaacaagata ttgctttaca
    10261 agctgaaaaa ttacttgaag gcttgaattc agatgctgaa acaaattatt atggtgaacc
    10321 tttagacgat gaagataagg aaaaattata cgatgccatt caacttgcac ttgccttaac
    10381 caaagttaaa gcaaagaaaa aattcactcc taaaaaatat cgtcaatagg tgggctttgg
    10441 tatgcattta aacaaatact ggatattaga agaaatagaa aaattatttg tagagtttgg
    10501 atctgatgcg attaatccat ataaaataat caaatattct gatatagatt taattgaaac
    10561 tgatttaggg aataaaacat taggacaaac gatacacaat aaaagatgtt atgtgatatt
    10621 aattaatcaa aacctatcac cttctatgaa aagatttgta ttatggcatg aaatagccca
    10681 cgttagactc cataaaggta tttgcacagc agcttttaga gcaaacaacc ttagttgtat
    10741 gatttatggt atagaagctg aagctaatat gtttgcaatt gaaatgttaa ctagaacaat
    10801 agatgaacaa gatttatctg atatgagtca gttccaaatt attgaatatt tagggcttcc
    10861 tcataatctt gaacatttag tattacaaca ttaaaaataa aaaagcccat gctttcacat
    10921 ggactctttc ctcatttctg agtaaactct acaataatta tatcaaagaa atgaggagtt
    10981 ttgaatgtta ttattgattg gac
//
LOCUS       DANKKG010000088        10986 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000088 DANKKG010000000
VERSION     DANKKG010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.088"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(<1..2121)
                     /locus_tag="PW004_001938"
     CDS             complement(<1..2121)
                     /locus_tag="PW004_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2726900.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWRIYDILCNNGFTKQ
                     SACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWDGKVYVQNLFN
                     AAGIKEPITSLDAQVRLLIWTFTNG"
     gene            complement(2118..2822)
                     /locus_tag="PW004_001939"
     CDS             complement(2118..2822)
                     /locus_tag="PW004_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2726901.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(2834..5143)
                     /locus_tag="PW004_001940"
     CDS             complement(2834..5143)
                     /locus_tag="PW004_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDL2726902.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            complement(5338..5802)
                     /locus_tag="PW004_001941"
     CDS             complement(5338..5802)
                     /locus_tag="PW004_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726903.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            complement(5802..6428)
                     /locus_tag="PW004_001942"
     CDS             complement(5802..6428)
                     /locus_tag="PW004_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2726904.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(6435..6800)
                     /locus_tag="PW004_001943"
     CDS             complement(6435..6800)
                     /locus_tag="PW004_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDL2726905.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            complement(6790..7128)
                     /locus_tag="PW004_001944"
     CDS             complement(6790..7128)
                     /locus_tag="PW004_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726906.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(7118..7444)
                     /locus_tag="PW004_001945"
     CDS             complement(7118..7444)
                     /locus_tag="PW004_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HDL2726907.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(7425..7703)
                     /locus_tag="PW004_001946"
     CDS             complement(7425..7703)
                     /locus_tag="PW004_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HDL2726908.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETD"
     gene            complement(7705..9069)
                     /locus_tag="PW004_001947"
     CDS             complement(7705..9069)
                     /locus_tag="PW004_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDL2726909.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(9082..9651)
                     /locus_tag="PW004_001948"
     CDS             complement(9082..9651)
                     /locus_tag="PW004_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HDL2726910.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(9623..10810)
                     /locus_tag="PW004_001949"
     CDS             complement(9623..10810)
                     /locus_tag="PW004_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDL2726911.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
BASE COUNT         3035 a         2397 c         1733 g         3821 t
ORIGIN      
        1 tccatttgta aatgtccaaa taagcaatcg aacttgtgca tctaagctcg ttattggttc
       61 tttaatacct gcagcattaa ataagttttg aacataaact tttccatccc atgttgctgg
      121 accaacaaga ggatatgaag aaccatccca ctgaactaat ccgtatgctg gtccacctat
      181 ttgaacagta tcaggatcaa aagttcctcc tgtttcttgt tgaatgttcc ccaatattcc
      241 acatgcagat tgtttcgtaa atccgttatt acacaaaata tcgtatattc tccaagctct
      301 cttttctgca tctgttttta attcatcagg atacccacca ggtgtttctc ctccgccaga
      361 tggaccgcca ttttgtcctg gtatcacttc tttacctcta acagtaagtt ttccctgaat
      421 atctaaatca ccgaaataat atgcttttcc gtttcctaat aacacaaatc ctttttctgc
      481 agttggagaa attaaaatgt attttccatc gccatttgta cgtattacca aagaattttc
      541 ctcaagaggt gtaggtgtta cagcatttgg aaatggattt cctgctgaat ctgttgttcc
      601 gatcgttcca atggattctt ttctattcca gaattccata ccttttttgg ttagctccat
      661 gatttttttg ttttcattcc aaatttgaag cgtgcctttt acaagtttta acacatcacc
      721 ataagcatta aaggaagttt cgaatacttc tgcatttatt gttcctactt tgatgaagtc
      781 agcaacaatt tctccttttg atgtgatagc aataccgaac gggccattta ctccgttgtc
      841 tgaataacct aagccattta aattccaacg ccacactcgc tttgcattcg ccacatttgg
      901 agtatccatg ataagaatct ctgatggagc tttttctgga cgaaaaacga catgcccacc
      961 agaattacca gtgatccatg ccgtcgcatt caacacattt tgaactaacg tttctgttcg
     1021 attgtcgatt ttctttttca attcttgtgc ttgagtattt actgccgaag tgtacagtga
     1081 taaatcatta cctaaaacaa tatccttata tttcccaagc gttgggaaat aagtgtattc
     1141 gaccatacgc tcttttatct cgatatctag ctctttcgcc ctaacatgag cgacatcacc
     1201 aaagtgtaaa gacgctagct cttgatacat gtcaccgtat tcgagtgtgt gttccaacgc
     1261 caccatactg actgtatgag tagcttttgg ctcatggata cgatcattgt caaataaggt
     1321 tttgccccat ttgatcaatt catcaactgt tttacaatct ccgttttcac gtttggcaat
     1381 acgtcgattc tcgttggtta ctccatcaat ttctaaataa ccatattcga taggttcttt
     1441 gtcttcatca taatcgttat caggaacacc gccaaccaaa tacaaactat tgacaattga
     1501 ttcttcatca atttcttctt cgattgcttc taaattgatg ccaaaatcaa tccgaaaacc
     1561 attatctgat ccaatctgtt ttaccagttt caaatcatag ttatccatat caagttccgc
     1621 gctagtcacg ccagtcaaat tttgattacc gttatttgaa ccaatgattg cttcaactgg
     1681 cgcgacttgt cttgctgtaa attggtgcgt tgtccctaca ttcgaaagat agttaaattt
     1741 ttgatcaaat gccaaactgt ttttaagatt ggtcatgatc tgagacccat tgccgttatc
     1801 cgtaaacgac ttgacaataa agttcttatt ggccataaaa ccaatatgcc gagctgttac
     1861 agaaacggag gtcaaattct ttttgacatt ataaatttca aaatattgcc atgatccatc
     1921 tggtaccttc gcttttataa aatttccttt ttttaaataa gagcgatact gtccgcttct
     1981 tgaatagtta ccataaaaac gatattggcc attcagcaca cgattgattt ctggcaaatc
     2041 ttcccaatca actaacgacg ctccgttcac gctcaaatca tctggcattt ttttataagc
     2101 ataaataaat tcttgcgtca cagatacaca ctcctattcc aaaaccgcac ttctttaaat
     2161 tttccggata tttttacctg attccattca ggctgcaata ctggccaatc accacgcgta
     2221 aataaattca aaccttcttg tattgctttt ccaagctgag tatcaaccac gattgttgca
     2281 gctaacgtat taagtattgt taggctttta tcaccgacag cgatcgttat atctcctccg
     2341 ttcgattcta tttccagata gggatgtgca atttcgtcac catgatcaaa aatatccata
     2401 acactagaat gaaaaatttt aggtgcttca ccgatttttc ttttgagtgg ctgacaacga
     2461 aaagtaacat cgaatgtata aaagaagccc cactcgtttt cgaaagggac ttctttatcc
     2521 atgctgcaga tagcctctag atacttgtct ggatcattat gtgtgattaa ttggctttta
     2581 ccagtgagcc atcgtttgac ttctctcagt ttcgaatgtg gaatagtgat cccttctatt
     2641 tccaaatcaa aaggttcata gtcatcaaac gtctccgtca attctccgct tcggccctga
     2701 atcgtgtaag tttcgtatcg cttgttcggc ataatatctg gaagctctgc ctcaatgatg
     2761 caattcatat caattactgc attccgattt ttccaaataa aatttggttc atctggattc
     2821 ataaattgtc gactcaaata ggaacacctc ccaaatcacg aatagcctgt ttgtttgctt
     2881 tagcaaattt acgattcata cgatctaact cagatggatt gtttgcatcg actttgccaa
     2941 tgtgtatatg ctgttcaatg ctacctccag aaactcttcc actgatccct ttgcgttttt
     3001 cttcgtctga taatggagta actgtggttt taccattctt ggcagtcagt aactcaggtc
     3061 cagcttcacc aacaatcgct tgtccattga ttagatgacc gccttgtgct aaataaggca
     3121 gttttgaaat agaaaatgat tttccaccta caccaggaac ccattttggt atctttatat
     3181 tattcaatcc accaataaat ccattgatca atccaatcat ggcattaata ggagctttcc
     3241 ctacagcaac aataccatcg aaaatacctc caaaaatatc aactagacct tgccatgctt
     3301 ttgaccaatt ccctgtaaat actccagtga taaaatctag aaaaccactg aaaattcgtt
     3361 ttccagcatt ataaaaatta ttgaaatttg ctataacacc atcgaaaaat ccaccaaaat
     3421 aaccacttaa aaaattaaat atttcaacag ctacatctga aacacctttg aagaaagaat
     3481 tcactccatc atgaaaccat ttaacgttat tatatgccca aattagtcca gtaactaatg
     3541 cgccaatagc tataactgct aatgcaaacc agcctcccga cattccaaat aaaccagcaa
     3601 gtccttgcca aactcctata aaacttttaa cttccccaac gatttttgta acagatccca
     3661 taagtgaacc aagaacgatt agtactggtc caactgctgc cgctatacca gcaattgtta
     3721 tgatccaatt ctttgtatct tgatctagag acccccacca tttagagaat tcctgtaata
     3781 aggaagttgc tttttcaaaa aaaggcaaaa ggcttacttg gactgcttca cctacgtcag
     3841 ccattgctaa ttttgcattg ttcatagctt gatcggcttg atcaattggg tcgagagtag
     3901 catcgaatgt gtctcctaca gctcccccac tttctccagc agtttttgct aaatcttcca
     3961 ggttcaaagt acctctacga attgcatcgg ccattctagg accgccttta gtcccgaata
     4021 cttctgctgc tgcattgatg gcttctgttt cagaacctgc attttttacc ttgtcttgta
     4081 gttcttctag tccttgactt aaagattttc cgtctttcgc ataagccact gttgctttag
     4141 ataaactact taaagctgcg cttgaatcaa caccagattg ctcaaattgt ccaagtaaag
     4201 tgaccccttc accaaagctc aaccctaatt gtttaatttg tggtgcgcca tcaattgctt
     4261 tttgcatcaa gtcgtctaca gattgaccag tattctgcga tgttttcgtt gttacatcaa
     4321 ggacagaatt caaatcatca tattctagtc cataagcatc aatagcttgt cgagcagata
     4381 ttgctgattg cgaaacatct gtatcattga tttcagcgta ctgtaggaga taattggttg
     4441 aatcttccag ttttttatcc atgaatccaa attgtgtatt tacctcacca atagcttccc
     4501 caaccgtttg taaaggtaaa tgagtatttg aaccaacgtt tttgaaagac tgtgaaagtc
     4561 tatcagcttg atcacctgtt gctccggttt ttgtaatgat ggtatcaagt gcctcgtcaa
     4621 cttcaccaaa tgctgcaagt cctgctgctc ctgccgctac aataggcgcg gttactccaa
     4681 cagacatttt ttcaccaacg ccttttactt tctcacctgt ttcttcgatt ttctggagtt
     4741 tttttgctgt atcaacagaa acgtcacctt gttctttaag tgcatcgttc gtctgttcta
     4801 atgctgtacg aagtttattt tctccggttt cagaattgag taattgttta taaagttttt
     4861 ctgattcagc agaatattca ccggtttctt tgacagattt ttcatattcc tctctcagca
     4921 tttgagatcg ttgttcagcc aatcctaatt gtttttctaa tttttgttta gccgctcgca
     4981 atttttctgt ttgagttgca tccttgtcca tcgcagacac ttggtttttg tactcggcag
     5041 cggctaagtt catttctttg ttgatttctt tgattgtttt cgaataactg acttcgccat
     5101 tcgttttaaa attaagaacg acatcagatt ctttaccagc catttatctt agcgctcctt
     5161 tctaccacca aggcgattta tccatagtta cagattgagg tggttcaaaa tcggtgtttt
     5221 gttgtaacca ctgtaaataa gatttgagcc acaagttagg tgtagacttt aaaaagaaac
     5281 tctcactcca attcaataga gtgagagcaa cgtaaatata aaaactccaa ggagttccta
     5341 tctcttccga ttctttttgt ttttctttct ttttgcttgc gctttttgaa agtcttgtgg
     5401 cttcttggat ttttttaggt cttccacctg aaatgtctgg ctagcaaaaa tttccataca
     5461 ggtactataa gcagacaaca cctctccact cattcccaaa aatttgaaaa tagtttctgg
     5521 atcttcctct aaaccaccag tacgcagcat ggcataaatc aaggcacgca tgatttttag
     5581 atcactaggc gataagttag cagaagaaat ttgtccttct tttttagata gcatggcgtt
     5641 catatcagat tcaaattgcg aataatctcc accatacaca tctgctataa attccatcgt
     5701 ttgcatagta aatgaaatag ggaattctgc gccttgaata attacaacag cagaattttt
     5761 taagtcttca acgtgaattc cataatcaga taaccgagcc attaacccgc acctccaact
     5821 ttagccaatg ttttccactg ttcttcatcg tatacaggtt gagcaatgaa tttattaaat
     5881 aattctagag aagctccgtc tctatttgaa tcaaaacttg catacatcac attattgtat
     5941 ttcagtccga tggaaaccag atttgcagtt acatcgtcaa tttttgtttc ttcctctgca
     6001 gtggcatact cttcgtcaat cacattggat agttgtgtat taggatacca aactgctttt
     6061 ttaccgccat tttcaatatt tccaataaat ccaaaggcga agtacggaaa ttctcgtgcg
     6121 gtatttttgc taaacgttac tccgctttta gcaactaatc ctttgatttc atccatcact
     6181 tctatcggaa tacccacgtg atccaacgca agttcatgtt ttgtttctcg cgacacacga
     6241 cgaaacattt tgctggatgc ccatttttct aatgctgttc cattcccttt gacaccgatt
     6301 tttgtggcaa ttggcaatcg taaaacttca ctataagtcg gtgcagttcc aacagaatct
     6361 ggcgttgcca tcatcgcaat taaaatgtcg tccaatcctt caaaataaaa agtatcttgt
     6421 tttcccaatt aaaatcaccc ttcccataaa tcaagtattt ttctagtcat aatttcttca
     6481 attttttctt tgttttgttc atacgtacca cttgcaaaat gttgggcacg ttgatttgtt
     6541 gtgccatttt cggcaaagcg ccagtaaaag gccgtctctt caaattgaac tttcacttga
     6601 tcgttttcaa ctactacttt tatttgatcg cgcatatgtt tcttttttaa gagcgactta
     6661 ggaatattag gaagcaactg ctttagataa aagttagctg cttcttctaa agattccaac
     6721 gataattttt tcggatcaac ttttgaaagg tttcctaaat aatccgatat ttcagaaaaa
     6781 ccattcttat tacttggcat tttccacaca cctcacatat gtgtaatagt tggttacggt
     6841 atcgtcgttc tcatcacctt gtatacctgt aaaatctgaa tatggaatgc cagcattttg
     6901 cagcgcttgt tcaataacaa ctaaatcctg ttctgttcca agtgtaaaaa aagagacttg
     6961 gtaataaggt aattttttat agactttacc ggatgccatt tttttgctgt tactcacatt
     7021 tgagtacaca atgtatggat acatcgtccc taatctggct ttgtctctga acactgctaa
     7081 ctttgttgat ttcagcgctg ttttcaattc atcaaagcta atcgacataa gctaaactca
     7141 actccatttc tcttgcatcg ggattcgtat aaatgcgagt aatgttatac gttacagaat
     7201 caattttgag cgcacttaat ttctctgtga tggatttatc ccatctgact ttaattcgtc
     7261 tgacaacgtc tgtcttggct tgctgtgata aatatttttc ttgagaagtc acaccgagtt
     7321 cttcatagaa tattagacgc ttaaattcgt aaattgtagt tggacgatcg tttccgtctg
     7381 ttcctgtttt gatgtctagc aattcggctt tccatctgag attattagtc tgtctcttcg
     7441 gcattttgaa tcgctccttg cacgataaat ggcgtcatgg cattcatagc tttgtcgagt
     7501 tcatcctctg aaactctata ttcataggca atgccggcaa ccatcaaaat aagatattct
     7561 tgttggccac cagttgctgt tttgacataa tcttttgcca tatttaaata aaaagagagc
     7621 aaagaatcat ccatgccctc ttcaaaatga atatgtgatt tgaatttttc ctctaaagac
     7681 aattctttag tttgctcttc catcttaacc accaactggt tttgtaattt cgtagcgata
     7741 tactgccggt tcaaatggag aataaaccaa ttgaccatct agcaagttgt aaatttggaa
     7801 tccaatttga tttttaccag agaatttttc aacaagtttt tgaatttcca aggcaccaat
     7861 aacttcttga attttaaatg cagaaaaatc gccaaaatat aaaactggcg tgtctggttc
     7921 acccttttta tctgctgcat ctgtccaatc cacaggatag ccaactaatt ggtaaccaat
     7981 tccaccttct gcttgtgtga atggccgcaa caaaggaaat ccatcatctg ttttcatttt
     8041 ttcaatggca gtcaaagcag ctcgattaat aataaagcgt ccctttttca tcacttctgt
     8101 cactggtgta tttttaaatt cgattaatgc atcatataat ttttgcccag cacctgaagc
     8161 agttagatct aaaggttttt taaatgctac agcctttttg gctaatgcac caggattttc
     8221 atttccagcg tcatcaccat tgaacatata attgatttct ttacgcacat aagctttttt
     8281 caattcttcc acaacaatat cttcaactgg aacaccagac atttttaata attttttagt
     8341 tactgttgcc aaagcatcga attcggcagg attaagcaaa atttcatcaa actgaatagc
     8401 tgtttcagca atatcagttg aacgctcttt cttgtttaca ttcgcatctg ctttcttcac
     8461 aagaattgga tatttgacat ctcctgatgt tcgcaccact gttccgtatt tacgaagtaa
     8521 attttcttct tgagcataag taataacttc agatgcaatt acttctggga cagtaactga
     8581 accgttgcca gcttcaatcc ctaaagctcg agcttctgct tcagaaatat ttccagctac
     8641 aaaattagca aatgcttttc gtagttgttg atctttttgt ttcttggtca tttttgcacg
     8701 tgcctccaat ccgttcttaa ttgatccaag taatccatct cgttgctgtt gagtaatcat
     8761 tccagaacga ttttctgaac tatcttcact gtctgaatct tcttcttgat cgttgtcttc
     8821 accagaacgg ctttcgtctg aatccgtact attcgattga tcatctgtat tgtcgtctgt
     8881 ttcatcagtt ccagaatctg cgcctaattc gtctttaatt ccgttcaatt catcaataac
     8941 actgtcaatt tcttctttca cggcttctaa atctgcttcg cgtaattctc ctgattcaac
     9001 tttttcacgt aattcagtca atcgttgctc acgacgtgcc ttcatttttt tcaataattc
     9061 tttatccatg tatttttcct cctacgcttc tagcgcttga ttaatttttt tgattaattt
     9121 ttttctttct tcgacgtttt tttctaattc atcacgactt cttaatgcag cttctgtatc
     9181 ttcgtatgct ggcaaaggca caatagaaac ctcgtataat tccacttcat ggattgttcg
     9241 aagcattggt tcagaattgt agtcccacgt ttcttcagtc ggcacaaaac caaaactaca
     9301 ttggttgatg tctcctcgtt ccatagattt aactaagtcc cttgctaccg ttgtgtccgg
     9361 taaatcaact tcgaatttca aaccacgttc atcttcttca aggcgaaggg ttccgctttt
     9421 agtacgtcct agcacattgg accaatcgtg attgaacaaa caacgaacat ccgaattact
     9481 aattgccttt gagaatgcac caggaacaat cacttcgctt aagtcatccc ataaaagtgt
     9541 ttgactgttg aatacagcag cgtagccgct aatggtcctc gtatgagttt cttcatcgga
     9601 acgtgttgaa agattggtga tgtcaatcgt gcgaatttcc ttcttcttca tttccatcac
     9661 ctcctttcaa gtcttgatca ttcgttggta aggaatcatc tgttgcattt ttctggccaa
     9721 tcctagataa gtcatttgaa atatagatag cttgtgtttc tggagtattc tgtttaggaa
     9781 aaccaagcat ttctgccacg ttatctggac ttgtaatccc ggtacgaacg atgttgtagc
     9841 caatatttgt ttttgttgag taaggaacaa aatccaaaat attaattttc cattccactc
     9901 tatagccaga attaggcata aaaaaaagag cggtgtaatg ttcgctcttg tttttcaata
     9961 ttggtttgat tgctttgttg tgcagataca tcatcgcttt ttcaatatct gtcttcatca
    10021 gcgattgata cgtatcaaca ttgattccta gaaattttcc taagtctttt ttgtaaacac
    10081 ctaaataatt caaaattgcc gcgtcatcaa caggactttt taatgtctcg atggaatatc
    10141 cttttcccaa aggaatcatc ttaacagaat gatcactctc attttgcgtt ccttccagtt
    10201 gatccaatat agctttgaca atttttgttt gggcgctatt atttggattg atgtgggcgt
    10261 ctagtttaag catgaacgca agtaaaccgc ctttagtata tttgtccgtc aaaacttttt
    10321 cagcgctcag aacgccttct agagtgtttc ttgcaagatc aattattcca gcacctttta
    10381 atgaatcagt tccgatgttc ttaatgtgtc gaatcatttg accaggtatt ttttgaccat
    10441 tcatttcaaa ttcttcttga agtcgttcat tgattttagt tgttacaccg tacgccaaat
    10501 gaagctgatc cctatccgtt aatgggaatg tctcaccatt gatcaataaa gtatttgttt
    10561 ccaatttggt aaattcgaat cctgtcaaat aatcattggg actcttcaaa atatttagta
    10621 agtgatgatc tttgacttcg ttgccgtcgg gaccaataac aattggtgtg gccaatgcca
    10681 cctgattaga tatgtcttga actaattcat aaacatcaga agattccatg atagatgaat
    10741 ccgtcacata tctttgacca taacgtgtgt agtgtccaaa aatatcctcg atatatccac
    10801 gtttttccat aaaagaatag actgcattcg ataaccgatc acgtaatttc aatttttttc
    10861 accgcctttc tatttatcta tagatggaac ttaggtaatc atctaactca tctgaatcaa
    10921 tatctgtcat ttgattcatc gtttccttat gaccacacaa aaacgccacg aacccatcaa
    10981 tctttt
//
LOCUS       DANKKG010000089        10817 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000089 DANKKG010000000
VERSION     DANKKG010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.089"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(335..1183)
                     /locus_tag="PW004_001950"
     CDS             complement(335..1183)
                     /locus_tag="PW004_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL2726912.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1182..2249
                     /gene="gnd"
                     /locus_tag="PW004_001951"
     CDS             1182..2249
                     /gene="gnd"
                     /locus_tag="PW004_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HDL2726913.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFVKSIYKKIFL
                     YNEGIRNGGAIMEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAY
                     QEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSIRRA
                     EEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGA
                     SGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEI
                     MEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEEDSFS
                     AKVVSALRNGFGGHEIVEKGE"
     gene            2251..3252
                     /locus_tag="PW004_001952"
     CDS             2251..3252
                     /locus_tag="PW004_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HDL2726914.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALIERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3249..3533
                     /locus_tag="PW004_001953"
     CDS             3249..3533
                     /locus_tag="PW004_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HDL2726915.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3566..4363
                     /locus_tag="PW004_001954"
     CDS             3566..4363
                     /locus_tag="PW004_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HDL2726916.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4377..5210
                     /locus_tag="PW004_001955"
     CDS             4377..5210
                     /locus_tag="PW004_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2726917.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5228..6085
                     /locus_tag="PW004_001956"
     CDS             5228..6085
                     /locus_tag="PW004_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL2726918.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            6107..6616
                     /locus_tag="PW004_001957"
     CDS             6107..6616
                     /locus_tag="PW004_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2726919.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6653..7054
                     /locus_tag="PW004_001958"
     CDS             6653..7054
                     /locus_tag="PW004_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726920.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7166..7519
                     /locus_tag="PW004_001959"
     CDS             7166..7519
                     /locus_tag="PW004_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726921.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7553..7996
                     /locus_tag="PW004_001960"
     CDS             7553..7996
                     /locus_tag="PW004_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDL2726922.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            8013..9098
                     /gene="uxuA"
                     /locus_tag="PW004_001961"
     CDS             8013..9098
                     /gene="uxuA"
                     /locus_tag="PW004_001961"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDL2726923.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            9091..9804
                     /locus_tag="PW004_001962"
     CDS             9091..9804
                     /locus_tag="PW004_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL2726924.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(10011..10160)
                     /locus_tag="PW004_001963"
     CDS             complement(10011..10160)
                     /locus_tag="PW004_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726925.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            10556..10756
                     /locus_tag="PW004_001964"
     CDS             10556..10756
                     /locus_tag="PW004_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL2726926.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3631 a         1707 c         2329 g         3150 t
ORIGIN      
        1 caaatgataa cctaaaatga ggccaaaagc cagtgttatc aaggaaatac ttaattcacc
       61 aaaatttttt ttaatttttt agaaaataag aaacaaaaat aataaaaaaa agaaaagata
      121 aattttttac tactgtatac taactttgtt atcgcatcta acaagtaact cgaataatac
      181 caatacaacc ttctccaaag atgcgatgac atacgttcta acccaccgac tattgatgtt
      241 ctgtaccacc cttgcagaac caaaaaagcc actaccaaat atttggtagt ggcttttttt
      301 gttttatgga tttttcgctt tttctgttat ggtgctattc tttgatttca gaaatagtat
      361 gtcgaacttt agcgatagaa cgacgtgatt ctttttcgtt gtaaaacatc aaaatcacat
      421 atagagagtc caaaatgctt agttgagaaa tacgggaagc taaagcttca gatcggaatt
      481 ctgtttcttc agaaatagaa ataaaaacga catcgccaag tttagcaagg gaagaattag
      541 cttggctagt aataacgatt gtttttgctc cagtttttct tacggtttca gcaatatgga
      601 ttgtttcttt tgattgccct gtgtgagaaa tcaaaacagc acagtctttt tccgtcaata
      661 aagaagcttc catcaattga atatggtaat ccgtgctgtg agaagttgcg ataggggaac
      721 gcaaaaattt atgataagcg tcagttgcta caatctctga accgccaaca ccgaaaaaat
      781 agacacgttt agattcattg ataatagtag ccgctttttt tagatccttt tgttgtaaca
      841 acttttttgt gtcatttaaa gttgaaatac tagactcgaa caccttttgc gccatagtga
      901 gctcagtatc tgttttctga atattttcgt ggatagaaat agcagataga tcattttctt
      961 ggatcaacat ggccatttta aaatctttaa atccattata accaagtttt tttgtaaatt
     1021 gaaaaaaagt agagtcagca atgcccagtt cacttgctaa attgctaata ggactatgac
     1081 ttactttgct tggattttct aaaatataat cggcgatagc ctgttctttt gggctgaagt
     1141 cgttgtacat tgttttgatt tgtagtctta tggagacgct catgagaatt cctcattttc
     1201 ataataagat tatcattttt tccgttctca ttaatgttaa agatacaagg agagtaggaa
     1261 aaaatcaaac aaaaaatatt tttgtaaaat ctatttacaa aaaaattttt ttgtataatg
     1321 agggtataag aaatggaggc gctatcatgg aaattggtgt aattggtttg ggaaaaatgg
     1381 gattgaattt agctttaaac ttaaaagagc accatcacaa agtacaagga ctcgacattt
     1441 caagtacagc tgttagtcag gcaagagaaa atgacattcc tgcttatcaa gagatctctg
     1501 aatttctagc tacttttgag cagaaaaaaa tcatctggtt aatgcttcct gctggagaga
     1561 taaccgaaaa catgttaaga aaactttttc caaaactcgg accaggagac attattattg
     1621 aaggcggaaa ctcttattat aaagattcaa tccgtcgagc agaagagttt cagaaaaatg
     1681 aaatcggtta cttggattgt gggacttctg gaggaatcga aggagcacga catcaagcct
     1741 gcttaatgat tggaggagat aaagaaactt ttctatccgt ggaacaatta ttcaaagatg
     1801 tagcaattga aagaggatat ctgtatgctg gtgcatccgg aagcggacac ttcttgaaaa
     1861 tgatccataa tggaatcgaa tatggaatga tgcaagcaat cggtgaagga ttccaacttg
     1921 tacaaaaaag tgagtatgct tttgacttgg aacaagtcgc acgtgtttgg aatcatggtt
     1981 cggtgattcg cggctggctg atggaaatca tggaagatca gttaaacgaa catcccaatt
     2041 taagtaatta tcggggaatc gtttctgcat caggtgaagc aaaatggacg gttgaaacag
     2101 cgctagaaat ggctgttcca gttccagtaa ttgcaactag tttgttcatg aggaatcttt
     2161 cacaggaaga agatagtttt tcagcaaaag tagtttcagc actaagaaat gggtttggcg
     2221 gacatgaaat agttgaaaag ggtgagtaaa atgccagatt atcatatagc aatcgttaat
     2281 tccagcagtt tcggaagagt ctttccacac cacttggaaa gattaaagtc agttggagac
     2341 gttcatttct ttactgttga tcaagagatt ccaggaaaag aactagcgga gttacttaaa
     2401 ggattcaata tcattattgc aagtgttacg ccattttttc gacgagattt ttttgaaaac
     2461 aaagatgaac tgctattgat ttcccgacat ggtattggtt acaacaatat tgatttaaaa
     2521 gcagcaaaag aacaccagac aatcgtatct atcatacctg ctttaattga acgaaatgct
     2581 gtagcagaaa ataatataac taatttgttg tccatgcttc gacgcacgac acaagcacaa
     2641 gcaagagtca aggacgatcg atgggaagat cgggcggagt tcgtcggtag gacgctgttt
     2701 aacaaaacag ttggtgtgat tggtgcagga aataccggaa gcggagtaat cgaaattttg
     2761 cgtaacggtt ttcgctgtga tgttctagct tatgatcctt acaaatcaga tttatacatc
     2821 gaaagctttg gtgcaaaaaa agtagaactt caagaattac tggaacaatc agatatcatt
     2881 tgtttatgtg cgaatttgac agaagagaac tatcacatga tttctgataa agaagtcgat
     2941 cagatgaaag aaaatgttta tctatctaat agtgcaagag gcgctctagt tgatgaagaa
     3001 gcgatgatta atggtctgaa aagcggaaag atcgcaggat atgccacaga tgttttagaa
     3061 gaagaaccgg gacgtcaaga tcatccgtat ttagcgtttg aacaagtagt gattacgcca
     3121 catatatcgg cctacacaag ggaatgttta gaagcaatgg gtgaaaaatg tgtcagagat
     3181 gtcgaacaga tcagtcagca ccttcttcct gaacgttctg tccagacaaa gagcaactat
     3241 ataaaaggat gatgaagaaa atggtgaacg gtttaaaagt gaaaacaggc cctcaatttt
     3301 atttatatga agaaggtggt atttcaaaag tttctgatct cttgaaatca tacggcgcga
     3361 aaagagtact ggtcacccac ggaacggtat catgggaaaa ggcgctacct aaattagtat
     3421 ttttgaatga cgaaacgatt caattttttt atcatcgtta ttctggagaa tgtagttatg
     3481 ctgaagccag acgaatagct acaatcatca aaaaaatgaa atcgatttcg tagttggcgt
     3541 tggcggagga aaactgaccg atctagtggg ttatagtgcc aatcttgcaa gaatcgattt
     3601 cggagtcatc cctactttag ctagtaattg tgctccatgg acacctcttg ctgtcatgta
     3661 taaggagaat ggacaagcag aagggaaaac agaacacttt tacagacaag ctgctttttt
     3721 tataacagat ccccaattag tgattgattc ccctgttcca tatttcattg ccgggttagc
     3781 agatacacta gcaaaatggt atgaatctga gctcatctta gaacaagatt ttttgcaaga
     3841 tgaatcattt ttgaagctag cacaagatac tgcgaagatc tgcaaagaag agattttgaa
     3901 tcattctgct aaagcaatcc aagatatggc acaaagaaaa ctgacatcgg agttcaaaca
     3961 gctttcagag attatctttg ctgttgctgg tttagttgga ggattaggag ataaatacgc
     4021 aagaaatgcg gcagcacatg ctatgcatga cgctatcagt aaatatattc cggaaagcca
     4081 tcgttttcta catggagaaa aagtagcgta tgggattttc tatcaactgg cactagaagg
     4141 tcgctgggaa aaaatagacg agttatatcc tttgtatcgg gaattagaac tgccggtttc
     4201 tctagaacaa atgggattat ttccgaaaga taaacagata atagataaaa tggctgaatt
     4261 tattgattcg aaagagaaag tgcatttgat tcctatagag gtaacacaga aatcactgat
     4321 acagtcaatg atggaactgg aaggttatat cagaaagatc tgaaggagga ggacacatgg
     4381 aaacattaag tgtagtacaa agtttgttga ttgctttatg ggtagccgcg gttatgtccc
     4441 gctggttagg tggaggagcc acgttgacct tacgtttttc tcctttgatg actggattag
     4501 tcgtcgggtt gatcatgggg gaagttgcac aagcgatgat tgttacagcc gctttgcaaa
     4561 tgatttatat gggggttttt tctccaggag gatcaatgcc agctgaacca tctattgcag
     4621 cagctatcgc agtaccagtc gcattattag gggatctgtc gccagaagca gcgattgccg
     4681 ttgcggtgcc agtcggattg ctcggaagtt acttgtatca gtttcgtttt ttcatcaata
     4741 cttttttagg taaatataca gacaaagctg tagaagattt gaatacaaaa gctatggccc
     4801 gatcaattat tctttatcca acggttgcct catttttatt attcgttccg cttgttttca
     4861 ttgctttata tttaggggcg ccattgatcg ctgatgtaat caaagcttta gagggaacag
     4921 tcgttatcca cattcttgaa gtagtaggtg gagggttagc ggcaatcggt atcgcgacaa
     4981 ctgtatacgt tatcggtcga aaagattatc tggtcttttt cttccttgct tattttatga
     5041 gtgttgtttt gaaatcttta gatattacga tggttactta tgccattttt gggattatta
     5101 tcgctttgat ctttgtccag tcgcaaaaag ggaaacaagt acagcctgcc acaagcaata
     5161 acaattcagc atcttttgat gacgaagatg actatgatga cggattttaa tttaggagga
     5221 atgggaaatg gctgaaataa tggataatca aacaggtgct cctgaagaaa tcacgaaaaa
     5281 agatgtaaca aaagcctatc tgagatggca ttttgcaaac gaaattcctc actcttttga
     5341 acgttacttg gcgccatcac tactatacgg aatgatgcca atcttgaaaa aactttataa
     5401 agatgaagac tctttaaaag cagcatataa acggcagtta ctgttcttca acactcaatt
     5461 aagctggggt ggcggtgtga ttactggcct aatgtcttct atggaacagc aaagagcaga
     5521 agaagagtat gaagaaaaag agatcttgat gcaggatgac ttgatgtata acacgaaagc
     5581 gggattgatg ggggcactag caggaatcgg agatgcaatc gattcaggaa ccgtccaata
     5641 tatttttatc gcaatcgcgg taccttgggc acaacaggga agtcctttag gcgcactttt
     5701 tccttttatc tgctttgggt tgtatcaggt tttactaggg gtatttttcg caagacaagc
     5761 tttcactatg ggccgtaatg ctacaggcct aatgcaaaac actggtgttc agacagcgat
     5821 tgaaatgcta tcagttttag ggttgtttat gatgggggtg cttgcgggaa actatgtaaa
     5881 agtagaatct tcattgaaat ttgcgatatc cggacgtgag tttgttatcc aggaaattct
     5941 tgatcaaatc gtgccaggga tattaccttt agctgtcgta atgggtgtgt acttgttcta
     6001 tacgaaaaaa ggattgaaag tcacacaagc attattatgg ctgactggaa tattaattgt
     6061 gctagcagga atcggtattt tataaaaaga agggaacgtg aataagatgg gtgtagtcaa
     6121 tttagcgcgt gtggatgagc gtttgatcca tgggcaagta atggtaacgt tatctcaaaa
     6181 aagtggagtg aattccattt ttgttgtaga cgaagttgta gcaaaagata aatttatgag
     6241 agatctttac aaaaatgcag gaagccgtac aggacaaaaa acaattgtca ttacaccaga
     6301 gaaagctaaa ttctattggg atgaatatca atttaaagaa tacaattgta ttttgatcgc
     6361 aaaaacagtc caaacgattt acgacttagt aaaacacgga atccccatgg aagaattgaa
     6421 tattggaggg attgctcaaa aaaatcctga tacagatctt ttagtgacga aatctgttta
     6481 tttaaataaa tcagatgctg aaaaattgaa agaaatgaat gaacaatatg gcgtaaagaa
     6541 tatttatttt caagccactc catctgcacc taaaacagga ttaaaagatg tattgaacaa
     6601 gtttgatttg tcataaacaa atgcttagaa aaaagataaa gagggcgaaa aaatgaagaa
     6661 gaacgagctg tttcgtgatt gggaatttcg ttatcgctat atttaccgta agcgccgtac
     6721 aaaaaagagt aaacagcgtt ttctttcagc attagtatca gatatttaca gtatgagaac
     6781 agatgtgaca gtcattgctt acgatacgct agcctaccga tcaaaaaata tttatgttgg
     6841 agatatcgaa aaagcagaga aagttatctg tacttattac gatacacccg ttcatgcatt
     6901 aggaagttat ttcatgtttg attggaaaga ccaaaggaaa aaaacaatct actctatatt
     6961 attgtccttc attttgttgt tcagtttagg ttggtgggga atgatgatat ataacaaaaa
     7021 tcctcatcac gtattcgatc tgctatccgt ataaacaggt atcactgtac tagcatttgg
     7081 gagctacttt ttcttattgg gaaaagcagc tcgcggatgg cctagcagac aaacttttat
     7141 tcgaaatact tcatccatat taacgatgct agaaatgatc cgtacgattg atgatccgag
     7201 tgtagcgtat gcgtttgttg atgaaggctg ttatggagaa aagggcttag acagcgtgag
     7261 atcgggtatg aaaaaagagg ctattctttt ttatctagac tctgttgggg cagatacacc
     7321 gctacagttt tcgggaaatt atttttcaaa taaagaacag tggctgaaac aagttgataa
     7381 gttgaaagaa aaaaacgtca attatatttt tagtgcaaga aaaaagcaag cgcagttctt
     7441 ctatttgacc aaaacagatt tacgagggaa aacatttaat tggcaaaatg ccaatcagat
     7501 tattgctctt tttcgataag taaaaagtgc aaaacaagga gtgagaagac taatgttgga
     7561 aatcatgatc gttacacatg gtaagttgag cgaaggactg aaagatgcgg cggaagtaat
     7621 tgtcgggaca tcgagccata ttgaaacaat gtgcttagag tctggggagg acatccaaac
     7681 attcggagag aaggtcaaag aaaaattaac tatgatcaac acagcagaac ccatgctagt
     7741 atttgttgat ctaattagcg caagtccgta caatcaagcg ctcttggcag tcaatgaact
     7801 gactgaagaa aagaaagcag ccacttatgt catagggggc gtgaatctgc caatgttgtt
     7861 agaagcaatc aatcatcaga tgcttcacac accgatagaa gaagcggtgg aacaaattgt
     7921 caaacaagga aaagataatt taactgtttg gcatgcttca gcggaagaaa aaaaattaga
     7981 tgaggacgat ttttagaagg aaggctgaaa agatgaagtg gggatttaga tggtatggtg
     8041 aaaaagggga ttcgatccca ttaaaatatg ttcgccaaat accgggaata aatgggataa
     8101 ttggaacttt gctgactaaa atgcctggag atgtttggga gatttcagaa attgaggagt
     8161 tgaaagcatc tgttgagagc caaggactgg aactgcttgg aattgaaagt gtagcgatcc
     8221 acgatgcaat caaggcaggg acagatgaac gagattacta tattgataac tatatacgaa
     8281 caatcaaaaa cttgtctcga tgtggggttc gattgatttg ttatagcttc aaaccaattt
     8341 ttggatgggc gaaaactgat ttgtttaatg aaaacaacga tggaagctat tctcttttat
     8401 acgatcagaa aatcgtcgat cagatggagc caggagaaat gtatcaattg atacacagcc
     8461 agtcaaaggg attcaaattg ccgggttggg aagaagaacg gttgaaaaaa ttcgctcagc
     8521 tggaaaaact gtatgaagga atgaccgaag aagaattatt tgaaaatctg acttattttt
     8581 tagaaaaagt gattccagtg tgcgaaaaag cagatgtaaa aatggctatc catccagatg
     8641 atccgccatg ggaacttttt ggttttccta gagtgacgaa aaatcttggc gacttgaaga
     8701 aaattctaaa tgcagtagat tctccatata atggactaac gctgtgcaca ggatctttag
     8761 gggcaaatcc ggaaaacgat ttagttgaaa tcatccatga ggtaggttcg cgcatccatt
     8821 ttgtccactt cagaaacgtg gagtttttag gagaaagaaa gttcaaagaa acggcacatc
     8881 cctctacaga aggctccctt gatatgtatc atattatgaa agcgttggtg caagaagggt
     8941 tcgacggtcc tatacgccct gatcatggta gaaccgtgtg gggtgaagtg gctatgcctg
     9001 gatatggcct gtatgatcgt gcaatgggac tcacttacat gcaaggtcta tatgaggcga
     9061 taagtaagga aaaaccactg ggagagcatt atgaataaaa tcaagctctt catctttgat
     9121 atggatggcc ttttattaga aactggacgt ttagcttatc gtgcttatgt aaaatcagca
     9181 caaaagtatg attatgaaat gcgtaaggaa gtctattatt tactaactgg ccaaacagaa
     9241 atggcgataa gagaacaaat gggcattttg tacggagaag atgttccata tatcaaatgg
     9301 cgagaagcaa tcaatcaata taaagaaaaa atcgtgaaag aagataagag agtatatacg
     9361 aaaaaaggag cagaagaaat tttatctttt gcaaaagaaa gaggaatcca tacaatcgtg
     9421 gcttcgtcta atacacgggt aaaagtagaa atgtatttaa aaatggaaaa tctatatgcc
     9481 ttgtttgatc aaatcatttc aggggatgat gtaaaaaaag gaaagccgga accagagatc
     9541 tttttgaaag cttgttcaaa aatgaatatt cctccaagtg aagcacttgt atttgaagac
     9601 tcgattgcag ggatcgaagc agcaaggcga gcgggcattt tgtcttttct catcaaagat
     9661 catttgatag ggttgcccga tcataatggg aagcataagt tgaaagtaga tgtaagttta
     9721 tatgaatcca ataaagcaga ctatgaattt tatgacctgc atcaagcaaa acgattttta
     9781 gaaagtaaag ggttgtatct ctaataaaga ggactatttt ctagtcctct ttatttattg
     9841 tcctgcacga ctgagggaac agatgaaaaa gaagtaattt tttattttga agtatctacc
     9901 ttatttgtag tgaacaattt agaacaaaca caaaacgcat ctactgttat ctaccagtag
     9961 atgcgtttaa agagaagaac gagaatcgtc catatgaggg tttccactcc ttatgcagat
    10021 ttttttaatt cttgcactgc ttgttcgatc gtaacacctg tcgctacatg attggtattt
    10081 tctttgtcaa aaacgacaaa tagattcaaa tctgtatcca aggttactcg atattgtata
    10141 tctttgttat agaatgtcat agccattctc ctttctttaa atacttgctg aaaggcaacg
    10201 aacgcgttga taacgaaatt ccatttataa gtataccacc aattgaaagg ttttcatagt
    10261 taagaaattt taaagtcaaa agttgcagat aaaagcaaat aaacaagaaa acaaaaagtg
    10321 aatcaatcgc ggttattcaa ctttttcttc gattcgaaga caatagaaga caatgaattt
    10381 tatatttcat tgcattatcc ttgtgttatt agaaaaagca tgatatatta aatgagtaat
    10441 ctattttgaa acgtaatttg agcgatatat ctacatagat aaaactcctt ttgcctatca
    10501 aatatttatt gcaacgaaaa aacgtacatt gatacgtatc aggaggaatt tatttatgaa
    10561 taacggtaca gtaaaatggt tcaacgcaga caaaggtttt ggctttatca ctggtgaaga
    10621 tggaaatgat gtatttgcac atttttcagc tatccaaggt gacggcttca aaactttaga
    10681 agaaggccaa gcagtgtctt acgatgttga agatggtcaa cgtggccctc aagcaacaaa
    10741 cattgttaaa gcttaataaa aattgacttt taaccgtctt ttcgaaagaa aagacggttt
    10801 ttttgtacga aaaaatt
//
LOCUS       DANKKG010000090        10689 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000090 DANKKG010000000
VERSION     DANKKG010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.090"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            136..1302
                     /locus_tag="PW004_001965"
     CDS             136..1302
                     /locus_tag="PW004_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL2726927.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            1532..2179
                     /locus_tag="PW004_001966"
     CDS             1532..2179
                     /locus_tag="PW004_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDL2726928.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            2263..3207
                     /locus_tag="PW004_001967"
     CDS             2263..3207
                     /locus_tag="PW004_001967"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HDL2726929.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            3342..4793
                     /locus_tag="PW004_001968"
     CDS             3342..4793
                     /locus_tag="PW004_001968"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HDL2726930.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            4814..5650
                     /locus_tag="PW004_001969"
     CDS             4814..5650
                     /locus_tag="PW004_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDL2726931.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            5650..6309
                     /locus_tag="PW004_001970"
     CDS             5650..6309
                     /locus_tag="PW004_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDL2726932.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            6435..7124
                     /locus_tag="PW004_001971"
     CDS             6435..7124
                     /locus_tag="PW004_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726933.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            7130..8086
                     /locus_tag="PW004_001972"
     CDS             7130..8086
                     /locus_tag="PW004_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HDL2726934.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            8130..8642
                     /locus_tag="PW004_001973"
     CDS             8130..8642
                     /locus_tag="PW004_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2726935.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(8898..9446)
                     /locus_tag="PW004_001974"
     CDS             complement(8898..9446)
                     /locus_tag="PW004_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2726936.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(9535..>10689)
                     /locus_tag="PW004_001975"
     CDS             complement(9535..>10689)
                     /locus_tag="PW004_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDL2726937.1"
                     /translation="LFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGFVLLRTTFGT
                     AVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISAIIGILRYIK
                     ILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFLSEKRLFTMS
                     VSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEEKEDELIIEE
                     NKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGITFQEILGYIF
                     APLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTAIISVFLVSF
                     ANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLLV"
BASE COUNT         3576 a         1920 c         2138 g         3055 t
ORIGIN      
        1 tatgcgacaa tgaatgtgaa aaaaatgaaa aataaagtag tttttgaaaa atttctgaaa
       61 aaattaaact tcttagttga ttcttttctt ttttagtatt aacgtaatag ggtagttttt
      121 ttaaggagga aaatcatgtt agagaaaact ttatacaatc aattattttc acgttctttc
      181 tcgctccctg tcgaagtaaa ttattgggac ggtacaacga aacaatacgg agatacggac
      241 actcctcccc aaatcaaaat caatatccat gaagaaatcc caatgaaaga gattacaagt
      301 aacgcttctt tggcactagg agaagcctat atggatcatc gaatcgagat cgagggaagt
      361 atccaagcat tgatcaacga tgcttatcag caagcaaaca gctttttacg aggaaaagat
      421 tatctaaaat ggctgccaaa gaaagaaaaa catacgaaac aaaaaaacaa agaagatatc
      481 catgcgcatt atgacttagg taacgacttt tataaacttt ggctagatcc aactttgact
      541 tattcttgtg cttatttcaa tacacctgaa gatacgttgg aacaagcaca aatcaataaa
      601 gtccatcata tcttagacaa actctttatc aaagaaggcg aaacgttgct tgatatcggt
      661 tgtggctggg gaacattgat gttcactgct accaaagagt ataatgtcaa ggcaactggg
      721 atcacactaa gtgaagaaca atacgatttg attaccaaaa gaataaaaga agaacattta
      781 gaagataaat gccgtgtatt attaatggat taccgcgaac taaaaggcga aacgtttgat
      841 catatcacaa gtgtaggtat gttcgaacat gtaggttctg aaaatcttga aggttatttc
      901 aaagtggtaa aagatttatt gaaaccaaaa ggaacagctt tgatccatgg aatcagccga
      961 caacaaggcg gtgcgacaaa tgcttggatc aataaatata ttttccctgg cggttatgtc
     1021 cctggtgtta ctgaattagt tgataatatt acaaaaaacg atttgcaact gattgatttg
     1081 gaaagcttac gtcgtgacta ccaattgaca ttagaacatt ggacaaagaa ttttcatcaa
     1141 gtccaagatc aagtcacgaa agaaatgggt gattccttct atcgaatgtg ggatctatat
     1201 ttacaagctt gtgcagcttc atttgaatca agtaatatcg atgtgattca atatttgctt
     1261 gttcaacctg gaaacaatga catcccaatg catcgtgcat aattgaggca aaagtactgt
     1321 ttaaagacta tttaaaaaga agctaagttt tatgatgctc cagaagtgaa cttttttgtc
     1381 taacttctag gattgtctct aaagcttagt ttctttttct ttatattttc ataataaaag
     1441 tttggttctt taatttttaa acatgacaga aaattttttc ttcgttatac ttgtaataga
     1501 atactatttt ataacaaagg ggatcgaatc aatgaaaaaa actgttattt atactagttt
     1561 atttgcagct ttgtttttag ctgcttgtca atcaaatacc aatcaaggca catctgggaa
     1621 tacgtcttcc agtagcgaac agcgctcttc acaggtatcg gactcctcaa caaaagcaag
     1681 cacatcttcg tctagccaga caaaaagttc tagtgaagca agtactcagt cagatatcaa
     1741 agtgtctgca gctcgtgcga tcgaattgtt ccaagataaa tacccagata ctgcaattac
     1801 tagtttggaa ttagatagtg attggggatc ttacttctac aagatagaag gtgtcgatga
     1861 tcaaaaagaa tacgaagcaa gaattgatgc tcaaaatgaa aaaatggaga cagagaatcc
     1921 agaaactcta gatcgagatg aacaaaatgg tcaaaagaaa aaagaagatg gtctggatgt
     1981 tgataaattg atcacgattg aggaagcagc aaaaatcgcc gtcgacaaag taggaagcgg
     2041 tacggcaaca gattgggatt tggataaaga tttaggtact acttattggg aagtcaaagt
     2101 aaaagacggc aatcaaagta catcagtcaa gatcaatagc caaacaggcg aagtactgtc
     2161 aactgaacaa gatgattaag aatttcttcg gaaaacaaga gttgattttt cttttagtca
     2221 tgatacacta gacaaaataa cttgtttgaa ggagcgattt acttgagttt agaaacgacc
     2281 tttgccaatc atatctttgc caatcctttg atgaatgcat ctggtgtcca ctgcatgact
     2341 acccaggaat tagatgaact ggctcattca gaagcaggag catttatcac taagagctgt
     2401 acgatcaatg aacgaaaagg aaatcccgaa ccacgctatt ttgatgttcc tttagggagt
     2461 atcaattcta tgggacttcc aaacttaggt ttttcttatt atctagagta tgctcttgcg
     2521 tatgaaaaag tgcaggaaaa ccaaaatcaa ccgttatttt tttccattgc cgggatgagt
     2581 gtccaggaaa acttggaaat gcttgagaaa atagaaaaaa gtgggtttaa tgggatcact
     2641 gaattaaatt tatcttgtcc caatgtgcct ggaaaaccac aactagctta cgattttgag
     2701 gccacttatg aaacactgaa agaagtattc agcatctttt ctaagccgct aggaatcaaa
     2761 ttgcctcctt attttgattt tgctcatttt gatcagatgg ctgatatctt gaatcagttt
     2821 ccattgacct atgtcaatgc tatcaacagt gtgggcaatg gtttgtatat cgatacagaa
     2881 caagaagcag tagtcatcaa accaaaagaa gggttcggcg gcattggcgg agagtatatc
     2941 aagccgactg ctttggcgaa tgttcgcgct ttttatacac gtttgaaacc agaaatccaa
     3001 atcattggta caggtgggat tcggacagga caagatgcat ttgaacatct gctatgtggc
     3061 gcaagcatgt tacagattgg tacagagctt cataaagaag ggccggaaat tttttctaga
     3121 atcataaaag aattaacgca gattatgtca gaaaaaggat atacgtcaat tgacgaattt
     3181 aaaggcaaat tacgcaccat ttcatagcaa agagacgaag gtcatgattg atcttcgtct
     3241 ttttttataa aaaacactcg tctgttcatt cctgtgcaag cgtactcatt gtatgttaga
     3301 cgaacttgtg ggaaaataac agaggcaaaa aggaggataa tatgaatgat gcaacagaat
     3361 tagcaaaaga gcttaaagaa aaagaattta gttttcaaga actagttacg gatatacaga
     3421 aaaaaataaa gaagcaaaat catgaattga atgcgtttgt cgcgcttgaa ccattcgaga
     3481 gtaattccca cgagctattg attccccagt cagaaaaagg gccattggct ggtatcccat
     3541 ttcctttaaa aatgctcggt caagaaaaaa agggatggct agctacttca ggatctcgtt
     3601 tatttgaaac acaccgagct tctcatacat ctaattatgt gcggcaagca gaagcgatag
     3661 gtctagttcc ttttggacaa accaatgcgc cagagtttgg tttcaaaaat attacagacc
     3721 cagtcatcta tggcccagca agaaaccctt ggaaccttga atgtacaccg ggcggatcaa
     3781 gtggaggagc tgctgctgca gtttcatcag gaatcgttcc tttagctggt gctagtgatg
     3841 gcggtggttc tattagaata cccgcttcgt tttgcggatt gattgggctg aaaccttcgc
     3901 gagggacgat gccagttggt ccatatgctt ggagaggatg gcaaggagct gctattgatt
     3961 tcggcttgac agtttctatg agagacacgg aggctttatt ttatgggatg agaagtatcc
     4021 attccggtgc gccatatcaa gtatcgccag ttgagtggca aacacatcca agaaaaaaac
     4081 ggttgaagat agctttttgt gttgattcac cgatccactc acagattagt gaagaggcag
     4141 tacttgctgt aaaaaaagct gtagatttcc ttggaaaaga agggcatgat ttgacagaga
     4201 ttccttaccc actagatggt cgtaaactga tcacgtctta ttatcagatg aatggcgcag
     4261 aaacagcagc aatgatccgc tcgattgaaa caggcatcca gcgagctgtc agaaaagaag
     4321 aacttgaacc catttcttgg actttgttcc aatatggtca gaagctctct gccgctactt
     4381 acatcgattc tctgcatgta tgggatgaag cagcaattgt catggaaaac ctacatcaag
     4441 aatacgatct attcctttca ccaacgactg catttaccgc ccctaaaatc agcgaagatt
     4501 tgcaaagtga tgcgataaga gcacagatgg aacacatcag tgattgttct gaagtagaag
     4561 gtttagacgt tgtttcttcc atgtttgagg agagcttgaa gatcacacct tatacacagt
     4621 tggctaatct gactggacaa ccagcaatca gcttgcctac tcatttgaca aaagaaaaca
     4681 tgcctctagg aatccaattt atggctgcca gaggaagaga agatttgcta tttcaaatcg
     4741 gctatctatt tgaagcaaat catcagttcc atttaccaaa tccatggaaa tagcagaaac
     4801 aaggagaaga agaatgaaga aaaaaagatt gtttgctcta ataactagtg ccttttttat
     4861 tttaagcgct tgcggtacag ctgccgctaa tcaggaagat aacagctgga gtaatgtaga
     4921 aaaaaagaaa gaattagtag tagcgacttc tggcacactg tttcccgcat cttattataa
     4981 tgatcaaaat gaattagttg gttatgatgt agatttggca aaagaggtgg ctaaacggtt
     5041 aaatgtcaaa atctccttta aagaatacaa cgtggatgga caaatcactt ctgtttcaaa
     5101 aagagaatca gattttgctg cgaacgattt tggtatcaca aaagaaagag aaaaaaaatt
     5161 ctccctttca gagactttca aatattcatt tgacagtatg atcgtacgaa aaaaagatga
     5221 ttcaggaatc cactcacttg aagatcttaa agggaaaaaa gcagctggcg aacccaatac
     5281 tagctatatg cgtttagcgg aaaaacacgg agctaaatta gtcacatatg acaatgccac
     5341 gaatgatcag tatttaagcg atgtagcaaa tggtcgaaca gatgttattc taaatgatta
     5401 ttatctgcag aaaatggccg tatctgcttt accagatatc cctgtaaaaa tactggagaa
     5461 cgtttatttc aatgaaaacg aaagtggttt tttattgaaa gaaggaaatg atgctctgaa
     5521 aaagaaaatc gatggcgtat taaaagagat gaaagaagat gggacgacaa agaaaatatc
     5581 tgaaaaatat tttcaaactg atgtttcagt caaaccgaaa gaaacgatgg ctgagtcggt
     5641 gagtgagtga tgtactttcc tctaattgat ttttccctca tgtggacttc acttcctttc
     5701 gtactgcaag gcttggtata cacattaggg attggcttcg tgtcgtttct tttaggaaat
     5761 ttggtagggt tgcttttgac agttttaggt cttttggatt ggctgcctct gaatgtattt
     5821 attcgttttt atttatcgtt ctttagaggc attcccgcac tagtcctgct gtttctgtta
     5881 tatttcgggt taccttatca attaagtgcc ttaacggctt cagtcatctg ctttacgatt
     5941 accagcagtg catttattgg tgaaatctat cgaggatcac tagcaggcgt ttcttcaggt
     6001 cagtgggacg ctggtttagc ttctggtttt tctttctttg gggtattgaa atatatcgtt
     6061 ttgccgcaag cttttcgaat ctcagtaccg gcactcagta acgtagctat ggatctagtg
     6121 aaaggtactt cgttagcagc aacaatcaca gtgccagaaa tatttcaaaa agcaaagatc
     6181 ataggcggtc ggacattcga ttatttttct ctctatattt tagttgcact gatttattgg
     6241 gtcatttgta tcgggatcga aaaagtgcaa cagtatttag aaaaacgttc tatccatcag
     6301 tcaatctgaa aaatagaaat ggtgctagcg tggctggcac catttctatt ttttgtttct
     6361 tgtatattct gcctagtatt ttaaaaccat ttcatgtaaa atgtaaatcg aaaatatgtt
     6421 cggaggtaga aaacatgaag accccgctaa ccatcgaaat agaatcctcg ttataccaat
     6481 attgcatcga gcaaggcggc ttggttgtag aagaagtcac gatgcctgat gaccaaggaa
     6541 ttgtagatac gcttagctgc ttgacaaaat ttgatggaag ccgagaatgg cgatgctatg
     6601 agctgaaagt gacaaaagca gattttcgct ctacagccaa actgtcattt gttggacatt
     6661 ataattattt tgttttacca agtaaactat atgaagaagt aaaaactgaa atccctacag
     6721 gaataggtgt tttagtgtat cgtccgtaca cggttgaaga ggagatgcct gcctctggta
     6781 catttctgat tgcgaaaaaa gcagcaaaga aagagcttgc tgtaccggaa gaagctttga
     6841 caaaccgctt catggcttct ttattcaggg aagtaagaaa agcaaagcaa atggagcgag
     6901 gcgtaaaata ttttccaaaa gatcaattgt acaaagaatt gaaaagacgt tcagaaaatc
     6961 aagatccatt ttctaacgaa cgatattatg aacggttttt agaggaaata gctagtcagg
     7021 caatcaatga gttgcaagaa gaagttatcg cgcttcagca tgattatgaa tatttgaggc
     7081 aacaacagca gatccgtcgg ttgcctacag agccattgga gtgaaaaata tgtactatcc
     7141 ttatttaaga ggaaaacaat ttgacctatt agcattaaaa gaagcactgt cgcgaggatt
     7201 gctctccaat aaaatccaac cagtgatcga accagtacgt gattccgcta cgcttaaaaa
     7261 tgtgatcgaa ttatttcaaa aaaagcacca tccaatcgct gtgatccaaa atccccaagt
     7321 aggacaattc aaattattcg atcagcatgt gcacagttgg acagtcaaag aaaatagctc
     7381 tgtcgtccct gcacagatct taacgccaga aaatctcagt gaagtactgg attcacctcc
     7441 agcgtttctt gttttcgatg gacagcacta cccaaaagat acagaggtgt ggaaacagtt
     7501 ggcaggagtt gattcgaaat ttttgatacc agatactagt cgctttcgca tctggctgcc
     7561 cgaaaacaaa attgtgatcc gtgactcttt tcaaacgaga aagcatgttg aaagttatgc
     7621 agataaaaca gatgactttt ttagtgatga ctatcttttt ttccacgcag acggatatat
     7681 tggattttct gatttcacga tcgaaggtag ccgttatttc gataaagggt tccctagccg
     7741 agcgcttgct atccatctaa cgtatattga tgcttacggc aatatacgcg tgaaacattt
     7801 tgtctctgac agtaacgact ctgctaaaga ccaagcattg aagtttcttg aagcaggaga
     7861 taaaatgaaa aaatggatca tgcgtcacca tcatcaactc ttgattactt caggaatgat
     7921 tgaattattg gctctttacc agcaacaaaa attccctgga ctgggagtgt tgaaaaagtg
     7981 gtcacttatg catcatttgg aacttattag tcacctgcta gatcatccaa aagattggct
     8041 taaatattat tcaaaagttc aggaactata tgaagtgatc caatgattag ataatgtaaa
     8101 tgaaaaagga atagaaaaga ggaaactgga tgtatattcg gcaaacaagg aaagaagaag
     8161 tagaagaagt ggtagaaatc attaatgccg cagcggattt cattgcttcg caaggctctc
     8221 ctcaatggca aaacgggcag aaaccaacaa aagaaacgat tttgaacgat attgaaagaa
     8281 aagaaagcta tgtattaata gtggaagatc aaattgctgg aacagctgca ttagtctcag
     8341 gcgtggatcc cgtttacaca aaaatagatg gtagttggaa agaatcaact ggtccttatt
     8401 tatcgattca tcgtgtcgct ttagcagaaa gtactcgagg aaaaaaacta gcaaaaccgt
     8461 tcttagaaca tttgttgacg attgcagcca gcaaaaatat ccaagatatc cgtatcgata
     8521 cttatccagc taatgaaccg atggaaaaga ccatctattc tgctggattc acttatcaag
     8581 ggatgattga atttcctttt gaacacggcg aaagaaaagc atatcaaata atcatcaatt
     8641 gactataaaa tcatctgagc aaagataaag acattaacac tactgaaaaa aacaatcata
     8701 tgatagccaa gaggttgtaa cagaagtggc cagcttcaaa aaacaagaaa gaatttccga
     8761 aaatgcttct caaatttttg tggaatttta gcgtatttta cgaaatagct gctttctgaa
     8821 actcccttca tcgagaggtc gtgacatagc ttttgtcacg atctctcggc ttatcctacg
     8881 agtattattc gatacattta tatcaataat ccttttttta ttttatctgc agcttcttgg
     8941 aacgctccat cgtttgacac gactaaaagt gaggggatag ctttcaaacg tttcaactgt
     9001 gctaaatctt ctacataaac cgccagtctg gctgaaatct gttcagatga atctccacgt
     9061 tttttcaggc gcgattgcaa tgtttcttta gagatcgtaa taaagatagg acggactacc
     9121 gtcccgaaat actcatataa atgccaaaaa ccatttgctg tcaaaacggc gtaacaattt
     9181 cctactgtca gtttcttttt aatttctgct ttactggaac catatttatg ttgagcatat
     9241 tggtcccact ctgcaaactc atttcccttg atcatctgat tgaatgtatt gtcagaaaca
     9301 aaataataat cctcttgatc tttttcccca ggtcttggtg atcgattcgt gtaagtaatg
     9361 atcttttgtt ccggctgaaa aatagcttct gcgagtgaag tttttccaga accagaagga
     9421 ccgataaata catagcaata attcattgtc atttctcctt tgatgattga acaagtgaaa
     9481 aagctttgga aaatatttcc aaagctttta aaagtaactt tgcttttttg tattttatac
     9541 cagcaagcca gtcacaatag cacttaaaaa gctgactaaa gtcgctgtta gcaaaatttt
     9601 caatccgtgt tttgcgacaa gatttccttt ttcttcattt aatcccttca tcgcaccaga
     9661 aatgatcccg atagaagaga agttggcaaa tgaaacgagg aagacagata taattgctgt
     9721 tgtacgctca gtaaaatgga aattaccatt tgctaattct gtcatagcaa caaattcatt
     9781 tgtaaccagt ttagtcgcca tgatactacc ggcatccact gcttctttcc atgggattcc
     9841 gctgatgaag gcaagagggg caaagatgta acctaggatc tcttggaaag tgatgccgaa
     9901 tatcccgtgg aagatagcat tgatcatggc aatcaaagca acaaaaccga taagcatagc
     9961 agcaaccgtg atcgccacat ggaaaccatc aagaatgtat tctcccaaca tttggaaaaa
    10021 tgtctgttct ttgttttctt caatgatcag ctcatcttct ttttcttcta atacataggg
    10081 atttacaatt gaagccagaa tataaccgcc aaataagttc aaaacaaggg cagtgatcac
    10141 atatttagaa tcgatcagtg ccatataaga tccgacgatc gacatagaaa ctgtagacat
    10201 agccgataca ctcatggtga acaaacgttt ctcagatagg aagggtaatt ccttttttat
    10261 ggaaataaaa acttccgatt gtcctaaaat agcagaagct actccattat aagattccaa
    10321 tttgcccatc ccgttgattt tacttaatcc tagaccaaca gctttcataa ataatggaag
    10381 aatcttgata tagcgtagaa taccgataat agcagaaata aatacaattg gcatcaatac
    10441 ggaaagaaag aaaggtgcac ttcctttatt tgctacaccg gcaaaaacaa agtcaacacc
    10501 ttgcccagca aaagcaagaa gatgatcaaa catttttgcc aatacagaaa caaccgctgt
    10561 accaaaagtt gtacgtaata atacgaaacc aagaacaagc tgtaaaacga acatcacaat
    10621 tagtggtttc aatttgattg cttttttgtt actgctgatc aaaaaagcaa gccctaatgt
    10681 gacaaataa
//
LOCUS       DANKKG010000091        10531 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000091 DANKKG010000000
VERSION     DANKKG010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.091"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            225..374
                     /locus_tag="PW004_001976"
     CDS             225..374
                     /locus_tag="PW004_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HDL2726938.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            385..1899
                     /gene="dltA"
                     /locus_tag="PW004_001977"
     CDS             385..1899
                     /gene="dltA"
                     /locus_tag="PW004_001977"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HDL2726939.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1896..3101
                     /gene="dltB"
                     /locus_tag="PW004_001978"
     CDS             1896..3101
                     /gene="dltB"
                     /locus_tag="PW004_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HDL2726940.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            3146..3379
                     /gene="dltC"
                     /locus_tag="PW004_001979"
     CDS             3146..3379
                     /gene="dltC"
                     /locus_tag="PW004_001979"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HDL2726941.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            3382..4650
                     /gene="dltD"
                     /locus_tag="PW004_001980"
     CDS             3382..4650
                     /gene="dltD"
                     /locus_tag="PW004_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HDL2726942.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4791..5873
                     /locus_tag="PW004_001981"
     CDS             4791..5873
                     /locus_tag="PW004_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HDL2726943.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5873..6676
                     /locus_tag="PW004_001982"
     CDS             5873..6676
                     /locus_tag="PW004_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HDL2726944.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6746..7486
                     /locus_tag="PW004_001983"
     CDS             6746..7486
                     /locus_tag="PW004_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL2726945.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7497..8501
                     /locus_tag="PW004_001984"
     CDS             7497..8501
                     /locus_tag="PW004_001984"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HDL2726946.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(8546..10390)
                     /locus_tag="PW004_001985"
     CDS             complement(8546..10390)
                     /locus_tag="PW004_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726947.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT         3405 a         1723 c         2117 g         3286 t
ORIGIN      
        1 gaataagagg ttgtgacacg atttttgtca caacctcttt attttttata ttttttaaaa
       61 gtacgagaaa ttataaagaa aaaagcagtg atttgatgtg ctatttttat aaaagctatg
      121 tgacaaaact gttagtaaaa aaacggtttc tgtaagctag acacatgttt ctttttgtca
      181 aaattagtta cactttgtgt gaggttatat gaggagggaa tccaatgaac aaatggcgtt
      241 ctaatccaaa attgaattat tggggaaaat tcatcattcg aacaattttt tatagtgcag
      301 tactgctgtt cctgatttat ctgtatcact ataaaaatat tcaaggagga acttttatct
      361 ataacgaatt ttaggaggaa gagtatggaa atcaaaacga ttattgaagc aatcgatgat
      421 tggggaataa atgaacctga tcgagttgct tatcaggcgg atgagacgca tacttttggt
      481 gaattgaaac aagcatcaga cgcacttgct tattatttag aaaaaaaggt agaaggcgat
      541 ggaccaattg tcgtttttgg caatttggag tttgaaatga ttgtctcatt tctaggggtt
      601 gtcaaatctg gccatgccta tttgccgatt gaagagcata cacccaagga gcgtatccta
      661 tcgatctttc gggtggctaa accatctatg gtcatcagta tcggggactg gatagaagat
      721 attccttcgg ttcctgtcat aacaaaaaaa gaatttcaag aaatcaagaa gataccagta
      781 ggattttatc ccagaaatag cgtaaaaggt acagaaaatt tttatattat ttttacttct
      841 ggtactactg gagaaccaaa aggcgtacaa atcagtcatg ataatctagt cagctttgtt
      901 caatggacga tggaagactt tgggatcaag cctggtatgc atttcttagc ccaagcgcca
      961 ttttcttttg atttatcggt attttctatt tatcctgcat tagtttctgg cggaatgctg
     1021 aagccgttaa ataaagcagt tgttcaagac tttcgtcaat tattcgcgac attgccagcc
     1081 ttgaagttga atgtgtgggt ttctactcca tcatttatgg atatttgctt gatggaacct
     1141 acattcaatg gagaaaatgt gccggaattg gacatgtttt tattttgcgg agaagaattg
     1201 acgaaaaaaa ctgcagaaag tctacttgat cgattcccaa atgcacggat atataatact
     1261 tacggtccaa cagaagcaac tgtagctatt tcaagtattc agattgacca gcatgtacta
     1321 gatagttatg accgactacc gattggctac gtaaaaaaag acacgaaagt ttctattgtc
     1381 caagaaggta aacctgtttc aaaaggagaa acgggtgaaa tcattattgc aggaccaagc
     1441 gtatcaaaag gttacctgaa caatcccgaa aagacagctg ctgcgttctt ttgtcaaaat
     1501 gacttagcaa gttaccatac aggggatgca ggtagactag atgaatatgg gcttttattt
     1561 tatgaaggac gtatggatca gcaggtcaaa ttacatggat accggattga actcggggat
     1621 atcgaacatt atctgctcca agacaaccga atcaaacaag ctgtcgtcgt accaaaatac
     1681 caaggaacaa aagtacagca gctggttgct tttgtcgtat tagaaaacaa aacgaaacag
     1741 ccggattttc agttgactaa atctatcaaa gaacaattac tagaagttgt aatggattat
     1801 atgattcctc agaagttcca ttatgtcgat ctgctgcctc agacagttaa tggaaaaatc
     1861 gatcgaaaaa aattaacagc tgaggtgaat cctgaatgat gttacctcat atgatccctt
     1921 atgcagagcc gttttatttt tttcttttag ccattgcgct tgtaccgatc gttctatctt
     1981 tattgatatg ggaaaaaaga ttaccagttt atcaatcagc agtgacactt ttctttttat
     2041 ttgtcagctt tggaggtaat tattggcaac agggagcggc gctgattggg tatgttttgt
     2101 ggcagacgat acttgtatgg ggctattttg tttatcggca aaggaaaaat cagacaggat
     2161 ggttttttgc cgccgttatt ttagcgataa ttccactgtt tttaacgaaa gtgactcctt
     2221 tttttgaaca tggaaaagca tcgttacttg gttttttagg aatctcttat ttaaccttta
     2281 aatctgtaca aatgatcatg gaaataagag atggcatgat caaaacatat aagccatttc
     2341 gttatatcca gtttctgctg ttctttccaa caatctcttc tggaccaatc gatcgctata
     2401 ggagatttga aaaagatcag ctccatccgc ctgttcgtga taaatatgtt gatcttttgg
     2461 gaaaaggcgt ccaaaatatt tttgtcggtt ttttatataa attcattatc ggttattatt
     2521 ttggtcaagt actgcttcct gtagtgggac gcattgcctt acagcatggt ggtatttcat
     2581 gggcattagt aggttatatg tacgtgtaca gtatgtattt gttttttgat tttgctggct
     2641 atagcttatt tgcagtcggt acaagctaca tgatgggata tcagacacca atcaatttca
     2701 ataaaccttt tttatcatgg aatatcaaag aattctggaa tcgttggcat atgacgctgt
     2761 ccttttggtt ccgtgattat gtatatatgc gattgatgtt cttcatgatg aagaaaaaat
     2821 tttttaaaag caagatcgta atgtcaaata tcggttattt tgctttgttt cttctaatgg
     2881 gtatatggca tggattgacg tggtactaca tcgtatatgg actgtatcat gcttgtctga
     2941 tctgtctgac agatgcatgg atccgattta agaagaagca taaagagcag ttgccttcta
     3001 acaaatttac gcatggctta gccgtatttt tgacatttca agcagtatgt tttagcttcc
     3061 tgattttttc aggattttta gataaacttt tttttagcta acgaatagtg acatattaat
     3121 ttatataaca aaggagaaaa ggaaaatgga agaacaaatt ttatcaatct tagaagaaat
     3181 aacaggaaca gatgaagtaa gaaaaaatcg tgatattgat ctatttgatg aagggttgct
     3241 tgattcttta gcatctgttc aattattagt cgagctagac ggagagctgg atatccaagt
     3301 ccctgtatct gaattcgagc gagaggattg gtgcacaccg aataaaatca ttcaacaagc
     3361 aactgcattg aagggttgag tatgaaaaag aaactttttg ctattttcgg cccggttgtt
     3421 ttagctgttg gcttactgtt tgtgtttttt acctcctcct atcaagtaga tatctctaat
     3481 ccagaaacga ttagacgtgc ctcttcttcc atgtcagtga acgtgttgaa aggagatgcg
     3541 ataaaaaatt atgcattatc tgaaaaacaa tatattcctt tttttggctc aagtgaatta
     3601 agcagaatca gtccatttca tccgtctgtt ctggcagaaa aataccagcg aaactatcgt
     3661 ccatttttgc tgggagcacc cgggacacaa tcgttaagcc aatatatgat gatgcgttct
     3721 gcaggagatg ctatgaaaaa caagaaagtc gtctttatta tttctcctca gtggttcgtt
     3781 aaaaatggtg taaaaactga ttactttaat acgtattatt ctgaacttca aacatacgac
     3841 tggttgtttt caatgaagaa agtaacgcct gctgatcgct atttagctag aagattatta
     3901 actttctcaa aagtaaaaga gaatgatacg ttgacggcga ttctgcaaac gatcaaaaaa
     3961 gggaagctac ctttacctga atctttgaat cagctgcgca gccaatggaa catgctgaag
     4021 agagaggatg aagtattcag caatattggg ttgaaagatc gacagcaaaa aatcgatcat
     4081 gagtcaaaac gattaccaaa acaataccaa gaaacagaat tatccatttt agcaaatcaa
     4141 attggagaac gagaaacgac gaataatcca ttcgggttga aaaacgattt ttatacgcat
     4201 cgtatccgag cccacgagcc agagctcaaa cagtcacaaa aaaattggga ttatcgtttt
     4261 tcgccagaat tttctgattt tcagctggtc ttggatcaat tagcaaaaaa ccataacgaa
     4321 gtattgttca tcatcccacc ggttaacgag aaatggagcg attataccgg actatctcaa
     4381 gaaatgttac aaggatttgc caaaaagatc aaatttcaat taaatagcca aggattcaat
     4441 cgaatagctg attttgtcaa tcaagcgggt accaactatt ttatggaaga cacgatccat
     4501 ctaggctgga aaggctggtt ggctgcagat caacagatca gaccgttttt agaagaaaat
     4561 catattactg ctagtaagta tcatcttgac gatgcttttt ttagcaaatc ttggcaacat
     4621 caaataccag ataaactaca attaaaataa agaaaacagg cgagtgttgc tcgcttgttt
     4681 tctttatttt tgaatattct gaataaaaga taaaaatacc aagtttgcct gaacaaaaat
     4741 cataagcaat ggtataatca caatatgaat atagaaatgg gggaaaacat ttggatacaa
     4801 aagaagaaca gctacttatt gatttaactt ctgcaaaagg agtacctgga aatgaagaag
     4861 aggtaagaga agtattcaga gaatatgcca aaccattcgc tgatgatatt ttttatgacg
     4921 gtttaggcag tgtgattgcc aaacatggtg ctggcggagg ccccaaggtc tttatttctg
     4981 gacatatgga cgaagttggt tttatggtaa caaaaataac cgaaaaagga ttcttggaat
     5041 tccaaactct tggaggttgg tgggggcaag tcatgcttgc tcagcaagtt gaaatcaaaa
     5101 cacgtgaagg gaaaatcgtc cacggtgtga ttggttccaa accaccacat gttttaacac
     5161 ctcaagtaag aaataaacct tatgaaataa aggatatgtt cattgatatt ggcgcatcaa
     5221 gccaagaaga agccaaagag tggggcatac gtcctggaga tatggtcact ccatatattg
     5281 aatacaaacg aatgaatggc agtaaatatt tactggcaaa agcatgggat aatcgtatcg
     5341 ggacagctgt ttctctgcga gtattagaga atctatctaa agaagcacac ccaaatgttt
     5401 tattcgcagg aagcgatgtg caagaagaag taggcttgcg aggtgctcga acaagtacac
     5461 accttgtaaa tcccgatatt gcttttgctt tagatactgg tacagcagga gacactccag
     5521 gaatgacacc aaaagaagca gattcgattt taggaaaagg cccacagatt ttgatttttg
     5581 atgcatctat gatcccgcat aaaaaattat tgaattttgt tattgatgta gcagaggaaa
     5641 tggatattcc attccaatat acagtgattt ctggcggagg aactgatgca ggacaaatgc
     5701 atttgacaag agatggtgtt ccgtctgtag ccattaccgt tcctgttcgt tatctgcatt
     5761 cccatacatc agtgattcat gaagatgatt atttgaatac agtgaaacta gtcacagaat
     5821 tagtgaaacg gcttgatagt gaaacagttg aaaaaattcg gagttatgaa taatgaataa
     5881 tagaatacct atttctgaat atcggaaaaa agcacgagtt tctttggaaa atcaatgggg
     5941 aatcaatact tggcttgttt tcttatctat aatatttacg agcgtcatcc agaacttttt
     6001 tgacggtttc agtccttttc cggaagaaag catgcagcag cacttattga gttttctagt
     6061 cgataattta cttttattcg cattcaacta tgcattgttt tacattgcgc tagtagttgt
     6121 acggggtggc agaccaaaaa gtaacctttt attttcagtt ttccaaagaa agtattttgg
     6181 ttcgatactt ggcatcaatt tgatcaattc agtcatcaac tggctactca acagtttaat
     6241 ctttctgccc atgtttttaa tcagcggagt gagtacttat atgcagctgc ttttcagttc
     6301 aaatgcggta cctacgggtg tcatgccaga agattttctt gatatcagct ttgttttatc
     6361 gatgataggg atgatttttg tttcagcact catcatgagt attgttggcg gtttattcca
     6421 atttgctgca tggacaaagt tcgactatcc tgatttgtcg gttattcaat gtttgaaata
     6481 tggctggttt ttattgaaag atagaattgg tacgtatatt ttgttgcagc tgtcttttat
     6541 cggctggtat ttgctaggag caatcgcttt gttcatcggt cttttgtggg tcattgttta
     6601 tgtgaatgtg tcagttgcag aattttatga acaagcacgg attgaaaaag gaggtccaga
     6661 cgcctatttt acttagcatg taagcagttt attcatcaaa aaaataactt ttgtggtagg
     6721 ataaatcttt gagggggaat gtacgatgaa attaactgtt ttaggatgct taggagcgta
     6781 tccttataaa aatcaaggga caacaggtta tttgcttcaa tctgacgggt tcaatttgtt
     6841 gatcgatgca ggtagtgcta cgctgatcaa attacaagaa caccttgatc cacttgatct
     6901 agatgctgtg atccttagcc attatcatca tgatcatatt gctgaccttg gtgtattaca
     6961 gtattactgg caacttcatc ctaagagaaa tccggagaca ttgctttcaa tttatggaca
     7021 cacaaaagat tccattcact ttgaagaatt gacgttggaa aatgtctcaa aaggacatcc
     7081 ttattttgaa gctgaagaat taaaaattgg tccttttctg attactttta tggaaacaat
     7141 ccatccagta gtatgttacg ccatgcgtat cgtagaagaa acaacgggga aagtattcgt
     7201 atttacaggt gattcaggtt atttggaaag tttcgctgat tttgcgaaag atgctgattt
     7261 gtttctagca gatacctatc tttttgaagg aaatgaacat catcatgccc atttcacttc
     7321 aaaagaagct ggagaacttg cgaaatcagc taacgtaaag aaacttgtac ttacacacct
     7381 acctcagttt ggtgatttag aacaattgaa aaatgaagcg caaaaggcag caggcaaagg
     7441 gattgatata gaacttgctg cagttgacaa agtgtttaac atttaggagg aagaacatga
     7501 tttttgtacc aaatgaaaac cacgacccaa gaatcaattt agcaattgaa caatatttgc
     7561 tgcaagaaat gcctttagat gaacctattt tgctctttta tatcaatgaa ccttcaatca
     7621 tcatcggcag aaaccaaaat acgcttgaag aaatcaatcg agattatgtg gaagaaaatg
     7681 gcattcatgt tgtccgtcgc ttaagtggag gcggagctgt ctaccatgat tttggaaacc
     7741 tgaactttag ttttatcatg cctgatgatg gggattcatt ccgtgacttt gcgaaagtaa
     7801 cacagccaat catccaagca cttcatgaat tgggtgttgc tggggcggaa ttgaaaggcc
     7861 gaaatgatct agtcattgat ggcatgaaat tttctggcaa cgcgatgtat gctaccaacg
     7921 gtcggatgtt tgctcatgga acaattatgt tcgactctga tatcaatgaa gtagtcaatg
     7981 cattaaaagt aaaaaaagac aaaatcgaat caaaaggaat caaatcgatc cgttctcgtg
     8041 taacgaatat caaaccattt cttccagaag aaaagcaaaa aatgacaaca gaagaatttc
     8101 gtcaagaaat cttgttgaaa attttcggcg tagagtcagt agaagaagta aaaacgtacg
     8161 aattgactga tgaagattgg aaaaaaatca atcgaatctc tgaggaatat tatcgaaatt
     8221 gggattggaa ttacgggaag tctccagatt tcaatttctc tcgccaaaaa cgtttttcta
     8281 tcggttcgat tgaagtccat ttgaatgtct cagaaggaat gatcaaagaa gcaaaaatct
     8341 ttggcgattt cttcggacta ggagatatct ctgacgtgga aaaaaaattg gttggacaga
     8401 aatatgataa agcatctcta tcaaaaatag tcagccaaat tgatgtgaag aaatattttg
     8461 gtgcgataga accagaagaa ttgcttacct tactttattg aaaataaaaa atagcggaat
     8521 ccacatatat ggattccgct attttttata caggattcgg gtaatttcga gagagttgaa
     8581 cagtgatccg atcaatgaaa gtatcttttt cctgcgttag tgttcctgtg taacgatccg
     8641 ctacgtcttt catcatcatt aagtcaaaac gttcctgatc atctaattct tctgtgatgc
     8701 ctcgactaaa taggtttgta tcgtaatgtg cactatgttc gcaacaaatg acgaaatgtt
     8761 cctcttgttc gtgtatcgac aaataaattt ttcgatacag tggattttcg atggcatgag
     8821 tttctctaaa ggaatgctct aaagcgtgga tcaaaagttg agccagatcg tcatcgttga
     8881 taggaagttc ttcgggaaga tctgcttgga aggttatctg aatattccgt ttggcagcta
     8941 acttctggta acgcgctaag ataagatttg tcacctggtg gttgcaaaaa gaacacagca
     9001 tttcgaaatt ttttgcttct tctaatatct tttccacata gtttttagct tttgtttggt
     9061 ccgtatgcat taaatcttgc agattctcca tctcttggac aaattccagg cgaagtgtat
     9121 ttacttcttg gatatgctgt cgaagctgct cataatgttg ctccaacaaa tcatttttga
     9181 tttttaagaa atctacttgt ctccgatctt tgtcggtatt taagatgtga tcgattagat
     9241 tgtagctgat aataacgatt agaatgataa tatataaaat cgtagaccaa ttgatcgttg
     9301 tttgatagat acctaatgcc gtttggatgt aaatcaagca atggataatg gctaccaaga
     9361 cgatccaagg tgtacaagct acataaaaac ggttattctt atatgcttcg ccaatgcatg
     9421 caagtgaagt gactaaagtg cttataacac tagcaatatt agcaatcggc attaattcag
     9481 gaagttctgc aggagtaaaa gttgcataaa gcagaacgcc gataaaaatc aaaagttgga
     9541 acgtacaaaa aattccgtac cattttttat agtaaaacat tcggctccaa tagtagcaga
     9601 tgatgaaagc tgacgttaag ataataaata aatgagataa aaaagaatga acgaatggtt
     9661 caaaaagaat accacttaaa atatcttctg atacagcttc aaatcctaat gccaaagcaa
     9721 agcaacttaa gagaataagt gagtattcaa gacgtttttg tttatacatc aagtagatcg
     9781 tatagaaaaa ggtccctata gcgataaaaa cagcgattat catcgtcaat acttgcggca
     9841 ctgattgtga ataaataaaa ctaatcaatg gtccagttgt tcctagataa actttcggtg
     9901 gcagtccagc atagttctga taaggagatg agactgtaag ggtcaatttt tttccgatat
     9961 aatttgctgg taaatctatc acagccaatg agagtccggg attgctttct tcctgataat
    10021 ctttccgttg gtataaaatc ttttcgccta gatatacagt aatccattga tggtttcctt
    10081 tgattagtaa atttgcttcg tccaacggtt tttcaagtgt cgtttccata atgaatgttt
    10141 cgtctttatc cacggtagga atcgtacgag tgtacttgct gtcaaactta aaatcaggat
    10201 catcttttgt atagaaaacc cacgaatctt ttatttcttt catttgattg ctgtccgcag
    10261 gagaaaaaat aaaataaaag aaataagcaa ttcctgttaa aaataacaat gataatataa
    10321 tgattatgct ccactttata cgtaaataaa aagattcttt tttattatat atgttattaa
    10381 aaaaagacat aaaggtattc ctttctaaaa cttgtaatgt tagggtaaac gttttttaaa
    10441 ctaacgtcaa ttatttataa taaaaaatga gataaagatg aggaaataga attagaaaca
    10501 tataattatt aaagggaaac atttttacag c
//
LOCUS       DANKKG010000092        10478 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000092 DANKKG010000000
VERSION     DANKKG010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10478
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.092"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(178..585)
                     /locus_tag="PW004_001986"
     CDS             complement(178..585)
                     /locus_tag="PW004_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HDL2726948.1"
                     /translation="MKFLRKAGVKIGDVIIIGILIIPSFIPLFVFSIEKENETTEDAI
                     KYAIVQIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVR
                     TGWISKPGQTSICLPHKLVISIEKKESKDYYIY"
     gene            complement(580..1784)
                     /locus_tag="PW004_001987"
                     /pseudo
     CDS             complement(580..1784)
                     /locus_tag="PW004_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180763.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
     gene            1910..2128
                     /locus_tag="PW004_001988"
     CDS             1910..2128
                     /locus_tag="PW004_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2726949.1"
                     /translation="MSQYCHLTILKRERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTNHLPKRKKIMLIEEKNADVITF"
     gene            2149..2784
                     /locus_tag="PW004_001989"
     CDS             2149..2784
                     /locus_tag="PW004_001989"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL2726950.1"
                     /translation="MVRHLFVDFQWSPEKISNRLKHEHMDFSISFITIYRAICNDLFD
                     TEPLSHGNKGLIRSLHHRGKTRHTKNHVESRGKICLTHSIHGHPKSADDRSDFGHWKA
                     DIVAGKTSGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEVLNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(2977..3216)
                     /locus_tag="PW004_001990"
     CDS             complement(2977..3216)
                     /locus_tag="PW004_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2726951.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYQFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(3240..4763)
                     /locus_tag="PW004_001991"
     CDS             complement(3240..4763)
                     /locus_tag="PW004_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HDL2726952.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLIQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKLNKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNEMHNWPTFYPASLSEHEIAQEMMAH"
     gene            complement(4781..4903)
                     /locus_tag="PW004_001992"
     CDS             complement(4781..4903)
                     /locus_tag="PW004_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HDL2726953.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKQNKMKKLA"
     gene            complement(4933..5916)
                     /locus_tag="PW004_001993"
     CDS             complement(4933..5916)
                     /locus_tag="PW004_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDL2726954.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVIADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLEKFNHPLVFESDSSENH
                     YNGKHYHQHHQEHEFKSILLTSDQDIKKDFLFQLIDRLILTNQGSLYRFKGLIALQDQ
                     DFIVAIQGVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(5940..6089)
                     /gene="rpmG"
                     /locus_tag="PW004_001994"
     CDS             complement(5940..6089)
                     /gene="rpmG"
                     /locus_tag="PW004_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDL2726955.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(6110..6487)
                     /locus_tag="PW004_001995"
     CDS             complement(6110..6487)
                     /locus_tag="PW004_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726956.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(6519..6698)
                     /gene="rpmF"
                     /locus_tag="PW004_001996"
     CDS             complement(6519..6698)
                     /gene="rpmF"
                     /locus_tag="PW004_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDL2726957.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(6713..6982)
                     /gene="rpsN"
                     /locus_tag="PW004_001997"
     CDS             complement(6713..6982)
                     /gene="rpsN"
                     /locus_tag="PW004_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HDL2726958.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(7505..9271)
                     /locus_tag="PW004_001998"
     CDS             complement(7505..9271)
                     /locus_tag="PW004_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2726959.1"
                     /translation="MQMIGGLTMKSFYKNKFALTNQGAEDLVKASISSFFVYCINMVP
                     AFIIMMLIDELVLENVKPPWLYFTVSFVTLLFMYWLLDREYENLYNSTYKESAHLRMQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKIVDQGTHSELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(9231..>10478)
                     /locus_tag="PW004_001999"
     CDS             complement(9231..>10478)
                     /locus_tag="PW004_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2726960.1"
                     /translation="VFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAK
                     VTSFKALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPK
                     ILAVDLLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNET
                     QIKNCDIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNV
                     DKGQIKIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDAL
                     RLAGCESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASID
                     PDNEYELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQ
                     VYKQLVTNYENANDWRLDNEKFL"
BASE COUNT         3239 a         2018 c         1526 g         3695 t
ORIGIN      
        1 aataagtaat ctaaccgact gttaactaaa ccattgcagg tcccgttaaa gatacttgtg
       61 cctgttgtca ccgcttcttt ttcttgtcca caagcgctta acacacttaa taaagcccct
      121 gctattatta ataataatat ctttttctta ttaaaaacct cctcatctat ttccagatta
      181 gtaaatgtag taatctttgc tttctttttt ctcaatacta ataactaact tatggggtaa
      241 acaaatactc gtttgaccgg gttttgagat ccatccagtt ctaaccgcga tttgatcagg
      301 actattatct tctttaactc gaattcttcc atctttaatc tcaacaatat tatattgtcc
      361 cttcgcagga tgataattca cgagtttatg attgatctta tccaaatcga agcgatccat
      421 ttccttgcca tcaatttgaa cgatggcata tttgatagca tcttcagttg tttcattctc
      481 cttttctata gagaatacaa ataagggaat aaaagaggga ataatcaaaa tgccaataat
      541 tatgacatcg cctatcttga caccagcttt tcttaaaaat ttcattaacg atatctggct
      601 aaacatatta gaatttttcc atggaaatgg tgaaattttt cggaaattat ctgcattaag
      661 agaatcagtt aaacaaccct caagttgttc cttaatacat tgttgatcca tcttcatacc
      721 aataaaaact agtttattaa tgcgttcacc aaacaacgga tgccaggctt ccttcaattc
      781 gggttttttt gtataatcat ttgacactca attttaggta aactagctac ccaataattt
      841 aatgattcaa tacttttatg gctacccgat gtttcaaaga tcgtcgaaaa atcactatgt
      901 tgggcaaacc aaagaaaccc ttttgctctt gtaatattgc caggaaaatt tttatccgta
      961 agatgggcaa atttttgtgc gtcaaggagt aaccgatttc gatagataaa acttgagata
     1021 ccatattctt ctgtttcagg aatatgattt tgatagccaa gctctaactc tttgaaccag
     1081 cccattgaat aggaagtctc atctatatta aaaagatttg tatttagaat tttcgataag
     1141 tctacttcac ctttaattgt cctaattatt ttggcattgg actgtaactt agcaattaga
     1201 ccgttaattt tgtaaatcgt cttggttgac actaagtcac tattatttaa aagtaatatg
     1261 ttacaacact cgaattgatc aattaacaag ccacgaatat cctgttcttc ttcaactaca
     1321 ttaccttcag cttttctttc ctgaatcttt tcacccgttt cgaaattatc ccaaaaacgt
     1381 tttacatcca ctactataat aatggcacca atctctaatc gttgggtcaa atcaatgtct
     1441 aaagggtttt tagctaatat aattgcttga ataattggaa ttggtttgct gattcctgaa
     1501 gcttcaataa caatttgatt caagtcagaa aattgcacta attgatctag ttcaacaatc
     1561 aaatcttcgc gtaatgtaca acaaatgcat ccattcggca tcgaaacaag tttttctcca
     1621 gttcttttaa aacctttctt gtcgactaat accccatcaa catttacttc ccctatgtca
     1681 tttacaatga cagcaatttc ctttccgtga tcaccgtaca gaatatgatt tagaagtatc
     1741 gtttttcctg aaccaagata gccagttaag attgtaatag gtattttttc atagggaacc
     1801 tctttctata ccttgaattt acactttaag tgcaacacat aaaaatgagt cataatcgat
     1861 atccaactag tataagtttg tcgagaacac tcctagaagg aaaataatta tgagccagta
     1921 ttgtcatctt actatactca aacgagaaag aatctttctg cttcacgaag aaggattttc
     1981 tattagtata attggcaacg acattcagca tagcccgttt actattttac atgaactgag
     2041 aagaaacaag aaaaaaacta atcaccttcc aaagcggaaa aaaattatgc ttatcgaaga
     2101 aaaaaatgcg gacgttatca cattctaaat gatccgtgag tttggtcaat tgttcgtcat
     2161 ttatttgtcg attttcagtg gtcgccagag aaaatctcga atcgtttgaa gcatgaacat
     2221 atggactttt caattagttt tatcacgatc taccgtgcaa tttgcaatga tcttttcgat
     2281 acagaacccc tctcacacgg caataaaggc ctgatccgtt cattgcacca tcgaggaaaa
     2341 acaagacata caaaaaatca tgtcgagtct cgtgggaaga tttgccttac tcattcgatc
     2401 catggccatc caaaatccgc agatgataga agtgattttg gtcattggaa agccgatatt
     2461 gtagctggca aaactagtgg tacttgttta gtgactttga cagacagaaa atcatgcttc
     2521 cttttagcag gaaagaaggt ccccagaaag aaagccgctt atgtgagcga gaaaatgatt
     2581 gaacttttaa gcagtattcc aaatgaggcg gtcaaaacga tcactccaga tagaggaaaa
     2641 gaattctcca atcatttcgc agttacagaa gtattgaata atatgccctt ttattttcca
     2701 gatcctcatg cccttggcaa caatgaacaa attaaaatac gaatagactc attcgagaat
     2761 acttgccaaa atcgaaagag atagctacgg tagataactc aacgataaat cagatgattg
     2821 aaaaactgaa taacagacct agaaagtgtt tagattggag aacaccttat gaagtcttct
     2881 ttgataaagt gttgcgcttg atttgacaat tcaagaaata ataaaaagca gatcaaatcc
     2941 aaataaggga aaaatgatct actctggtat tatcaattaa ttggccaagc tattatgccg
     3001 ttttgctaaa cgtctacttc ttccagtatt gaccttgata aaacaacaat caggatcccg
     3061 ttttaatact ctatgaactg tgtcaatact aacatgtaaa gttgcagcaa tatcagctaa
     3121 tgtactccca tttaatctca tttgctttgc aaattgataa cgccgataag catcaatatt
     3181 cttggaatcc atagacaata atacttttct taccatgtat ttcctccaaa gattggattt
     3241 taatgggcca tcatttcttg agcaatctca tgctcagata atgaagctgg atagaaagta
     3301 ggccaattat gcatttcatt ataaagtttt tcttgacttt ctccaccaaa gaaaatatgg
     3361 aaatgctctg ccttacttgg tgcaattcca tgatcgctaa attgaatata tttaaaacga
     3421 ccagcacctg catcatcagt ttcaaaatta aaacgaacac cacggtttcc tttttcataa
     3481 ttcaaaattt tgtatccaac atatttgtat gtgtattgat gatgttcacc gttgactaca
     3541 aaatcaatgg tgttgtcttt aatgttgata gtatcaatat ctgtcttata gccagtagta
     3601 taataatctt tgtactcggc agctgtcatg tctttattga gttttgcttt gtaatcgaaa
     3661 acttcatcca aaattccatc ttttaacaac ggataaacag attgccattc gcctgtataa
     3721 tcactcaatt cgcgatcctt cacatcggca tctttaaagt acccattagc aactgtcttt
     3781 tcatccttcg cttctttttc tggaactacc tcttcaccag caaccatagt tgttttctct
     3841 aacgctttta gattgtcttc cattactgaa acataatctt caccattatc catttgttct
     3901 tttgtaagac tttcaagagg attcaatact tccaatttta tcccagcttc attggccaaa
     3961 gtttttgcga ttttatcatt agcattcttt tcaaagtaaa tataattgat cttatttttt
     4021 ttaacatact cttttaattc agctaatcga ccagaagatg gctcttcttc aggacttagt
     4081 ccagcaatag gaacttgtat caatccataa tccaaagcta aatatccaaa ggctgcatgt
     4141 tgtgtcacaa aacttttttg tttcgcttct ttaagactag tggtatagtc agcatccaac
     4201 cgtttcagtt tagtcaaata cttttcagca tttgtctcaa atacttttgc ttttttcgga
     4261 tacaatttaa ctaactgatc cttgatattt gaaacttcct taattgccat tttgggtgaa
     4321 acccacgtat gaggatctag ttcatgatgg tgatcttctt ctccgtgatc atgatcttct
     4381 tcttcactac ctggcaataa aatcatgtct tttgttcctt ctactacatt aggcccagct
     4441 ttcttccaac tttctttagc ttttggtacc catgattcca tgttttcgtt atgatataca
     4501 aaaacatcag catcatgagc ttttgccata tccttagctg agggctcata gtcatgaggt
     4561 tctgatcccg ctggaaccat taaatccacg ttcgcttcat cgccgacgat gtttttggtg
     4621 aaatcataca ttggatagaa tgttgttaca atttctaatt ttccatcttt atcattcgat
     4681 acttttgcat cattagtgct ggagcaagct cccaataatc ctagagctgc tatcccaata
     4741 agtcctacac tgattttctt cattcttttt ccttccttaa ttatgctaat ttcttcattt
     4801 tattttgttt atgttctttg ataattttta atgccctttt tctagttttg atattcgggc
     4861 ttttaagtcg tcgatatgca ctagatatat ccgttttctc cattagaaat tacccctaat
     4921 caatacttat cattatttgc tttccgccag taatttatca aaactctgtt tgatcctttc
     4981 ttcatccaac atttttccaa ttagaataat agtggtttgg tctttttgtc gagtggtgta
     5041 ttgaaaatta acctgttcgt ttaccccttg tatggcaact ataaaatctt gatcttgcaa
     5101 agcaatcaat cctttaaaac gatacaggct cccttggttg gttaaaatca gccggtcaat
     5161 taattggaat aaaaaatctt tttttatgtc ttgatctgaa gttaagagaa ttgatttaaa
     5221 ctcatgttct tgatgatgtt gatgatagtg tttgccatta tagtgatttt ctgatgaatc
     5281 actttcaaaa actaagggat gattgaattt ttccaaatta taaaaatcct ttttttgaat
     5341 cgtttcacta tgtgtgaaca ttcgaatatc ggataaaggg ttaatacttt tgatttctct
     5401 ctgtaatttt tttagtgcag ataaattcaa gttttgtttt ctagacacaa aaatgcgatc
     5461 tgctatgaca agttgcttaa gggcttgtgg ttctctttca ataagttcaa aactgtcact
     5521 atcaataaca gtcaataaac tatcgatata tactcttcct cttagttgag gcgctgttaa
     5581 aagtgtttga atgattggtt ggggatctgc tatacctgta gtttcaatga tgatttgtga
     5641 aattggttgt tgatgttctt ctattgtttc cagtaaagaa atcaatattg ctaataaatc
     5701 actgcgaaga ctgcaacaca tacatccatt atttagttgt aacacccgtt cctcgctatg
     5761 aactaataat ttgtgatcaa tcccaacttc accaaactca ttttcaatta taataatttc
     5821 ttccctgggg tagggtgaga cttgcaaggc ttgattgatt agcgttgttt ttccagcccc
     5881 taaaaaccca gaaacgattg taactggtat gcccattttg tcaccttttt accacttagc
     5941 tatttcactt ctttgaaaat tgcccgtctg cgcaattttg gcgaatactt ttttaattct
     6001 aaccgttcag ggttattccg tttatttttg ctggtgagat acagacgttc tccagtttcg
     6061 acacattcca aaataatatt ttgacgcata aatcttcctc cttcaatact tatcgatctt
     6121 caagatatgc ttgaatttgt tgtgcatctt caaaagttaa ctcgccagat tccaattgtg
     6181 aaactaaatt ttctttcata tcaacaggta tctcccaata accatcaata cattgaaata
     6241 actcctcgtc atactcttca aaaattggat agattcttaa aaaatcatca aatgataagc
     6301 cttggtcctc atagtaatct ttgactaatc cattttcaat tgcctgttgg gtcggtaaac
     6361 ccgtttcagt aataattcct ctctctacca tcgaacgata aaaagtgcat ttatctagcg
     6421 attgctgggt cgtttcaaga taatacttta atccttcttc aactaaaggt gggtaaataa
     6481 tttccatttt gattccctcc taaaagttat atattaaact attttgaagc tcctttaatt
     6541 actttctttc cattatagta cccttttaaa gatacatgat ggctacgtct ataatcacca
     6601 ttcgcttcgt caaatgaaat ttctggtttt actacagttt gatgagttct tcttaaccgt
     6661 ttcttcgcct tagatgtttt tcttgctggt actgccatga tatttcctcc ttttaccaac
     6721 ttgctttttt tactccagga atcaaccctt gatgggcaag ttctctaaac ttaatacggg
     6781 acataccgaa ctttcgcata taccctcttg gacgaccatc cgtctgatca cgaagcctca
     6841 gacgattcgg atttgaatct cttggcaatt tagcgagagc ggtgcgatct cctgcggctt
     6901 ttaattcctg acgtttatct gcgtactttt caatcatcgc catttgtttc tttgctttag
     6961 caattttcga cttttttgcc atataccttt atactccttt tattttgatt tagacatctc
     7021 aaatcgtaat cgttacgttt tgacactgat actttaccaa caagagttgc tcttgtcaac
     7081 aataaataac atgaaataaa actcatatga atagataact atttgaaaac gaatttatcg
     7141 gtacatagag ataatcaaat catttaaaag aatatgcagt ttaagctctg aaaaataaga
     7201 attcactttt attagtccaa tcaaacacaa ataccgtttt aagtagcttt tcctctactt
     7261 aaaacggcaa tatgaaagaa actataactt tttattcaat cttttatgtt taactggttt
     7321 atcgccaatt ttttaaaact ttgattattc cttatattat aagaatagac attaagtgaa
     7381 ctaattcaat catactgatt ctttcgtgta ttcaaaatta gatgctgttt aataaaatta
     7441 ttctttttga aaaagaatta taactatatc ccaaaaattg taggttaatt taaagcctat
     7501 ttcatcatcg tgattgagct tctttttcat agacgaattt ctcagatagt ttagttttct
     7561 ttatgagatt tttgtatatt tctgaccttc gatacaactc actatgagta ccttgatcaa
     7621 ctatttttcc ttgatccatc actacaattt tatttacctt ttcaatagac ttcaagcgat
     7681 gcgatataat tagtactgtc ttatcttgta ttaaacgatt taaactttcc tgaatacgtt
     7741 tttcattttc cgcatctaaa ctggcagtaa tttcatctaa aattaaaatt ggcgcatttt
     7801 tcaaaaaagc ccgtgctatg gataggcgtt gtcgctctcc tccagaaagt gaacttccgt
     7861 tttctccgac caatgtatga taaccgtcag gtagtttttc aataaagtct tcgcagttag
     7921 ctaaacgagc tgcttttttc acttcctcat ctgacgcttg actatttcca atacgtatat
     7981 tttccaaaat acttgtatta aacaaagtta cctcttgaaa aacaatagcg attttgctaa
     8041 acaaagaagc tggagataca cgtttaatat catacccatc aattaaaata cagccttcat
     8101 cataatcata cagtctagac actaatttca agatactagt tttcccggaa ccactagcac
     8161 ctactaacgc agtcacttct ccttgtttag ccgtaaaaga gatatgatct aaaattgggg
     8221 tattgttatc atatgagaaa gagacatctc gcaactcaat gtcaaaagat ttcagaggac
     8281 tatctgatcc ctcttggata ctggtttcct tcatagctct aattcgctgt attttaggag
     8341 ctaaataaaa tatttcaagc actgcttctt tcatagaatc gaaggaatcc tttattttta
     8401 ttgcggctag taagtaacca acgacgtaga gtatagtcac ttcacccgtt aacagtaagt
     8461 gaacacctac tagaatgact actgctaaac taacatatga gaaaatagag gataaggcca
     8521 tgactgaaaa agtacttaat tctaccttta aatgaatcct ttcactctct tccatttttt
     8581 tgaaaagtct gtcttgaact tttttagata gattaaagct attaatctct tgctgcaatt
     8641 ctatagtttc ttgaaattct tccgagtttt ctctcaaagt atcgtaatat ttagtattgg
     8701 ctttcgtttg ggatttcttt gataacaaga ttaagacaaa actaaataac gttggcaaaa
     8761 taacagctaa tcccattttg acattaccaa ccaaaagcat cactgaaata aaagggaaaa
     8821 acagagccat accgccggct ttaggtattg catgactcat cgcatgctca ataccttcaa
     8881 cgtcagacat gatagtttga gataaatctg ataaattatg ttttgaaaaa taggataatg
     8941 gtaaatttga caaatcgtct gcaatttgca ttcttaaatg cgcactttct ttataagtac
     9001 tattatataa gttttcatac tccctatcta acaaccaata catgaaaagc aaagtaacga
     9061 acgatactgt aaaataaagc caaggcggct ttacattttc taaaactaat tcgtctatca
     9121 gcatcattat aatgaatgct ggtaccatat taatgcagta tacaaaaaag ctagaaatag
     9181 atgctttaac caaatcttct gcgccctgat tagttaaagc gaatttgttt ttataaaaac
     9241 ttttcattgt caagcctcca atcatttgca ttctcataat tagtgaccaa ttgtttatac
     9301 acttgatgtt tagccattaa ttcattatga tttccacgtt ctactatttt cccttctgac
     9361 aagacaagaa tttcatctac agatttaatg ctggataatc gatgagctat cataataact
     9421 gttttttctt tcatcagatg ttgaaaagcc ttttgtagtt catactcatt atctggatca
     9481 atagatgcac tggcttcatc cataataaca atctttgcat cttttaaaat tgctctggca
     9541 atagctatac gttgtttttc tcctcctgac agataaactc ctttactacc aattaaggta
     9601 tgctctcttt cgggaaattt ttctaaaatc gattcgcaac cggctaatct tagcgcgtca
     9661 aatactttac ttgctggcgc ttgaggattt gctatagaaa cgttatcgta aatactctga
     9721 ttgaatagtt tcgtatcttg aaagacaaaa gcaattgtgt taataattgt gtcttttgaa
     9781 tagtgattta aaggcttacc accaatttta atctgtccct tatccacatt gtagaaacct
     9841 gaaattagtt tagctatcgt actctttccg cttcctgatt ttcctactaa tgcataaatt
     9901 cttttttgtt ctaaaactaa gttgaaattt tctaaaacac actcttcttt gtatgaaaag
     9961 ctgacatttt caaatgcaat gtcacaattt ttaatctgtg tctcgttccc atattttagt
    10021 tggtcttgct tcatttctaa ataaagggag tctagtgttt cgactgcata tcttgcttca
    10081 aacaagtgca tacttacata cattattctc atgaaagaaa caaaaataat tccacttaaa
    10141 aaaaacaaca tcaacaagtc aacagctaat atcttgggag tggtaagaga agttataaaa
    10201 aaggttatag gaactatcaa aatgcaaact atgccaaaaa atagccattg aaaaagaaca
    10261 aaaggtgttt tcccactcaa agaatactta tatgcaaact tagcataatc gttaattgct
    10321 ttattcaagg ctttaaatga tgttacttta gcaccaaata tctttataac ttgaattcct
    10381 cggatatatt ctaccgtttc agagcttaac acatctaacg attcttgata tttttgaata
    10441 aaatttgtat tctctgtcat tttctttaac aagaatac
//
LOCUS       DANKKG010000093        10422 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000093 DANKKG010000000
VERSION     DANKKG010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.093"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(<1..684)
                     /locus_tag="PW004_002000"
     CDS             complement(<1..684)
                     /locus_tag="PW004_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDL2726961.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDG"
     gene            830..1633
                     /locus_tag="PW004_002001"
     CDS             830..1633
                     /locus_tag="PW004_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HDL2726962.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            1811..2758
                     /locus_tag="PW004_002002"
     CDS             1811..2758
                     /locus_tag="PW004_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDL2726963.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(2826..3119)
                     /locus_tag="PW004_002003"
     CDS             complement(2826..3119)
                     /locus_tag="PW004_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HDL2726964.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(3147..3812)
                     /locus_tag="PW004_002004"
     CDS             complement(3147..3812)
                     /locus_tag="PW004_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HDL2726965.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(3952..4584)
                     /locus_tag="PW004_002005"
     CDS             complement(3952..4584)
                     /locus_tag="PW004_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2726966.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(4591..5658)
                     /locus_tag="PW004_002006"
     CDS             complement(4591..5658)
                     /locus_tag="PW004_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL2726967.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(5655..6386)
                     /locus_tag="PW004_002007"
     CDS             complement(5655..6386)
                     /locus_tag="PW004_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HDL2726968.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(6555..7034)
                     /gene="greA"
                     /locus_tag="PW004_002008"
     CDS             complement(6555..7034)
                     /gene="greA"
                     /locus_tag="PW004_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HDL2726969.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(7170..8345)
                     /gene="mltG"
                     /locus_tag="PW004_002009"
     CDS             complement(7170..8345)
                     /gene="mltG"
                     /locus_tag="PW004_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HDL2726970.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            8549..9718
                     /locus_tag="PW004_002010"
     CDS             8549..9718
                     /locus_tag="PW004_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HDL2726971.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(9985..>10422)
                     /locus_tag="PW004_002011"
     CDS             complement(9985..>10422)
                     /locus_tag="PW004_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726972.1"
                     /translation="GTYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVI
                     YSSFSPNKDEITFTFKSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKSES
                     GINKVTIRYSYTFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
BASE COUNT         3208 a         2072 c         1616 g         3526 t
ORIGIN      
        1 cccatcgatt gcttcaattt cttgttccat tttttcatag tcgatagcag gtgcactttg
       61 tatcaaaata gcggcagctt ttttgatcac cattatcgct tctatcaata tatatatcga
      121 aatgatcagt gtaataactg gatcgatcca gtagatatcg aaaaaacgga tcaaaattgc
      181 tccgataatc acacttaccg aagacagtgt atcacttaac aaatgcaaat aactagattt
      241 tatattcaaa ctttcatctg cttcttgtcg taataataat actgagaaca aattcgcgag
      301 caatccaata actgctactg ttagcatcaa atgactattt atcttttctg gtgaagtgaa
      361 ccgcttgaat gcttcaacgg ttaaaaacaa ggataatatg atcaatatga atgagttgat
      421 gaaagcactc aatatttcta aacgctggta gccatacgta cgttttttat ttgcttcttt
      481 tcgggccatt ttttgtgccc cgtaagaaaa aataattgcc aatgtatcac taaggttatg
      541 gaaagcatct gaaacaagag acaaactccc tgacataatc cctccaataa attctgataa
      601 agtgatcacc gcattcaaaa ccactactcc aaatagtttt tttgatgatc tctgtggctg
      661 tgtaggcact attccttcct ccattttatt tatattggat agtctttaga caagaataac
      721 atttttttga tatttcacca atcattcagc tgtttcctta aagcacttgt atttcacttg
      781 atacttcgct agaattgcaa gtgatgacac tactagatga ggagtactta tgacaacaaa
      841 acaattaatt atcagttcct ttatacgatt caaagaccta aaggaggcga gaaatgtccc
      901 attttggaaa gccattgtct atcttttagt tttgagtata attatggctt tgccaatctc
      961 ttttcaagtt tttcaagtct taaacaacat tcaattggat gggcaaaaga ttgctactcg
     1021 cattcctgat tttacaatcc aaaacgggca gcttcaaacc gaagaaaaag aaggattcat
     1081 ttaccaaaca aactccatta tcttcacatt tgatccagag ggaaaacgaa cagaacagga
     1141 catctctact gatttgatgg ggaattttgt tagtgtcggg atgttaaaaa acaaacttat
     1201 catagctctt cctaatacag gcacgacttc tgctttgttg aacaataacc aattagaact
     1261 tccctacacc aatgaatctt taaaaaactt aacaggtaag cagctccgtt cttttttaag
     1321 tgaagcatct ataccacttt ggataaaagt cctgacattt atattttcta tttatcctag
     1381 ttttctgaat ctgctgatca ctttgctatt taccaacatt gctgcttttc tctatgcacg
     1441 ctttcgacta acaaaagcta cttttcttga ttgtttaaaa acattgatct attcaatttc
     1501 attgcctact ataattgcaa caatcttgat ggtctttctt ccttcttttg acactagcgc
     1561 cttcattgct tttgcaggaa tctttatttt tgctcaagct gtcaaaggat gggcaaaaat
     1621 atcgatcaat taagaaataa agacaactcg ttatttctac ttcaatagac ccttttagag
     1681 gttgtgacat ttttttgtca cagccccttt tttatttgaa aaatatttct ttctcaaaaa
     1741 acaagcaagg cgcttcaaag ttcttcttgt atgcgctaca cttagagtaa agaaaggcgg
     1801 gaaattaact atgtcagaaa gaatcattta tgcagatcgt ccatttcgtg atgaatttat
     1861 cgaagaaatc catactatag cgcctggtta tatctttaaa acagaattac aaccagagga
     1921 tctacaaaat gtagagatca gtttgggctg gaactcagac taccaagaaa aactattagc
     1981 ctcttctacc ttgaaatggg tccaatctat ctcagctggt gttgatcagc taccccttga
     2041 gactttttca aagaaacata ttttactttc aaatggtagt ggcatccaca gtgaatcgat
     2101 tacggagcat gtcttaggaa tcatccttgg gtattctcgt ggtctttttc aagcacaaaa
     2161 ggcacagagt gacatgaaat ggttaggttc atcggtccat taccaatcta tccaagggaa
     2221 aaaaatacta atcattggaa ctggccatat tggaaaaatg attgcaaaaa aatgcaaagc
     2281 attagacatg aactgctatg gaatcaatac atccggtcat ccagcagaag gaatggaaaa
     2341 gactttttct ttaaatcaac taaaggaagt tcttcctgaa gtagatttcg tagtcaatat
     2401 cttacctcta acggaggaaa caactgggct ctttgatcag catatgtttg aacactttga
     2461 tccaaaatct gtattcatca atgttggccg tggtgcttct gttaagacac aagatctggt
     2521 gcaggcttta aataaccagc aactttcctt tgcagcatta gacgtttttg aggaagagcc
     2581 tcttccacaa gatcatcctc tttggaaaat ggacaatgta ctgattactt cccacattgc
     2641 aggtttgaca cctgattttc aaaagaaatt aatggctatt tttttaacca atctgaaaag
     2701 ctactttgcc acccatgagc ttcaaaccaa tcaagtaaaa ttgtctgcag gatattaata
     2761 gctgaaaaca atattttcaa aagaaaaaag agaaggtgac aaaaattgtg tcaccctctc
     2821 tttttttatt gttttttaaa gtgataggtt ggctcctcca ggttgggatc ataatcaaca
     2881 agtaaatcat atcctttaaa gaaccattca tctgcttcct cgataaaaaa aagtgtccca
     2941 tcaatttcca ctttctttac aggatgttct ggctgttcaa ctgacattcc aatcgaaaaa
     3001 ccatcatgaa cctctgtttt gccgtagact tttccaaaaa aacgtattcc gtaagtttct
     3061 ggcagatcaa tttctgagtc aaaccacttt tgtgcttttg gcgttaccgt taatttcatc
     3121 ccaacaacct actttctctt ttctttctat tgattcatct tatcgtctag aatatccaca
     3181 tcgcctgtct ctccaataac cagtagatta tcattttcac gaacatattc atctgctgca
     3241 ggagaagcga taaccgtttg atctactctt cgtatggcga ctactgttaa gtcaaaacgt
     3301 tgcctgaaat ttaattcagc taatgttttg ttgaaaaatt taggattgga gactcggatt
     3361 tctgctaaag aatatttatc tgacaactct aggtaatcta aaatattctt tgacactaaa
     3421 ttatgcgcaa tccttattcc catgtctcgc tcgggatgga cgacgcgatc agctccgatc
     3481 ttttcaagta ctctggcatg atactcattt tgggcttttg ctaaaatatt tggtacgccc
     3541 atttccttta ccatcagagt aactaagata ctggcttgaa tatcttctcc aatagcgaca
     3601 attacatgat cgaaatttct tatgcctaat gatcgaagtg tcatttcatc ctgtgcattt
     3661 gccacaacag catgagtggc tatattcata tattcattga cccgatcttc attactatca
     3721 atagccagta cttcttgacc agcttcgatc agtgttcggc agatacttcc gccaaatcgt
     3781 ccaagaccta caattgcaaa attttgtttc accgttttcc tcctttagta tcttcttttc
     3841 tcagtataac aaagccttta caaaaacaaa agcaagcatc attattgtaa aaaagccaag
     3901 aaaaataata gttattttct cagcttttga ttatcctctt tatcatcgtt tttatttcac
     3961 caaaccatgt ttgaatgcat agatagcggc ttgtgtacgg tcttccactt ctagcttagc
     4021 caatatattg gaaacatgtg tttttactgt tttcagtgta ataaacaatt cgtccgctat
     4081 ttcctgatta ctttttcctt cagaaatcag cattaaaatt tcattttccc gattcgttag
     4141 ttcttcatgt aatacaggct catttcgccg actcattttt tccatcattt ttgttgtcac
     4201 ttctggttct aatactcgct cgccacgttg cgttgccctt atagcatcag caatttcatg
     4261 tgcggtagag gtcttcaaca aataccctgc tgcacctgct tcaatggcag gataaacttt
     4321 ttcgtcatca ataaaactgg ttacgataat gattttagct tctggccaat cttttaagat
     4381 tgcttttgtt gattcgatac catccatttc ttccatgact aagtccatta aaatgacgtc
     4441 cggtcgaagt gtcatcgcct tctcatagcc ttgtcgtcca ttttcagctt ctccaattac
     4501 ttcaatgtct tcttgaatag ataaataaaa agagacgcct aagcgcacca tttcatggtc
     4561 atctactaat aaaactttta tcatccttat ctatgcctcc ttcatcaaag ggaccttgat
     4621 ttctacactt gtaccttgac ctttaaaact aatgattttt actgttccac caataccagc
     4681 tactcgttct ttgatattgt tcaatccata acttcccgtt ttcgtttcat tcatattaaa
     4741 ccctgttccg tcgtcaataa tacgtaacaa aagattattg tctattttgt gcaaatatac
     4801 ctctaattca ttggctttag catgtcttaa tgtatttgat aacaattctt gaacgattcg
     4861 gaacaagtga tcctcaatgg aactagatag ttttacatct tctacatccc atttcagcga
     4921 aatctggatt ttattttgca attccttcaa taactgttcg ataccttgct ttaaactttt
     4981 ctcttctaaa ttgactggac gtagatgaag aagcagcgca cgcatctcag actgggaagc
     5041 gttgatgatc tctgctacca ttttcaactg cttgcgaaac atttctggtg tctcgctttt
     5101 ttctgcttgc tccgtcaatg ccgacatcat catcattgct gcaaacaatt gttgcgaaac
     5161 tgaatcatgc agctcacggg ctaatcgatg tctttctaat tctaaaattt cttctttggt
     5221 ttgtccatct acatattggg gacggctcgt tacaatttgt aattcactag atacttcaat
     5281 cattttttct tttatcttag tgatttcctt atcaatatct ttgatgtata aatcgtccga
     5341 tgcacttggg atcggaagaa gcaacaattt ggattcataa ttgccagaag aaagtgcacg
     5401 cagcttttct tcgattttcc cgtattttgt cttttgaata agtgataata atagaaagac
     5461 aattaatcct acacctaagg aaatggctaa caaatggaag ataagcggaa catataataa
     5521 acgtgcacgc agtaattctc cccaccaatg actttgattg ctggcataaa aataagtgaa
     5581 aagtgaaaat agaataataa ggaaagccgc aatccccgaa taaacagcta gcatcgctct
     5641 ggatattttt cccatcatat tcgtatcacc tcgacatctc caatcaaggt attagtaata
     5701 atcttcaatc tacgagggtt ttcatcataa tcactgctgt agattttgat ttgttcattt
     5761 ttcaaagaaa attgctcttc ttcaaacaag acatttccta ctaatgtcgc atgctctaat
     5821 cggatggcta cgcccaaagg tactaagatc cgtgttctgc ctatcccttt tcttacaatc
     5881 acaatattat catcttttgg caaaagcgta ttgcccaaat ctatgatagt atcaccagaa
     5941 atgacggcga tgttgatatc gtcccattca tagacgtcgt ttccaatacg ttggttaccg
     6001 aataattgct gtcttttccg ctcattatta tgtgttttta attgctctgt ttggatcatc
     6061 atgatctgtt tctttcgcca aaaagcattt ttggttaaat ctatccctga aatctctaca
     6121 cctttcagac caataaataa aacggcaaat acagccatca tccataaagc aggactgttg
     6181 actaagctaa aaaaaatgac caaacttcct aatatcaatt gaaaattttg aaatttagta
     6241 cgaggatgtt tgcgcatagc tagatatata ttgaaaatac cgaatatcac taaaagcaaa
     6301 agtccagtat tgtttacgat ttgccaaaca gcaaataaca gcagtagtgc ttcaactacc
     6361 acaaaaaaac gccaagaact attcatcgct ctttctccct cctgacacat tctcatttca
     6421 tcatacctat ttttttaact gttggctata ggactttaga tgtaagatcg tttgttgaaa
     6481 tctaaaatac acaaagagaa aaagatctag agtgtatcat cagtaagatg aactctctag
     6541 atctttcagt cagactacgc ttgttctact ttgacgattt taactaacat atcgccacct
     6601 ggagtagcaa tagttacttc atcattttta cgtttgccaa tcaatgcttg cgcaatagga
     6661 gaatcattgg aaattttgcc agaaaacgga tctgcttcag cgcttccgac aatcgtgtac
     6721 tcttcttctt ctccatctgg tagttcgatg aatgtaactg ttttaccaat tgatacctca
     6781 tctgcatcta caccgtcgtt atcgatgatt tgagcaaaac gaatcatatt ttcaattgtt
     6841 gtgattcttc cttcaacaaa tgcttgttca tctttcgctg attcatattc agagttttcg
     6901 gaaagatctc cgaaaccgcg ggcaattttg attcgttcaa tgatttctcc gcgtttgact
     6961 gtttttagtt cttctaattc tttttctaat ttttctttac cttctagagt cattggataa
     7021 actttctcta ccatgtgtaa cgtctccttt tttcgataaa ataccgcaaa aggtaatctt
     7081 ttgaggaata tagtccaaaa aagattacca cgcttttctt caaatgtaaa tgtttttcta
     7141 aaaatgatca atgtctagag cctgtttttc taagaattat ttacatattt ttccactaat
     7201 tccatatgtt cttcataagt tttagcgaaa taaacatttc cagtgctcgt atctgctacg
     7261 aaataataat agtcattttc tgctggttct aatactgcct tgattgcctc ttcgcttgga
     7321 ttgtcaaaag gtccaggtcc atagccggta ttggtataca aattatatgg tgaatctaca
     7381 gctgtatctt catacgtcac taattctttg tgttcaccta atgcgtataa aacagagata
     7441 tctgactgaa gtggcatatt tgctttgatt cggttaaaga agacttgtgc aatgttttta
     7501 cgatcatttt cttttacgcc ttctttttca actagagaag ctagagttaa aacttcttgt
     7561 acagtcaaat ttttctgctt gatagctgaa taatagtttt gcatgaccgt attcgtcttg
     7621 ttcaccattt caatcactag atccttaagc gaagtactcg tgtaataatc atatgtggct
     7681 gggaataagt aaccttccaa acggtatttt gtgttctcag ctttagatgc actttccaac
     7741 agttctggga atttttggtg caactcgtta aagaaagtct catcattcac taaatctaaa
     7801 aattctttct tgtctttacc agttaccttc gctacccgtt cagcaatctg atcgatatcg
     7861 tacccttctg gaatggcaat tttcccatct gcaacttttg ttggctcaga agtaccgcct
     7921 tcttgcaact gtttgccaat ctcgtcaaga gtcatattag gcgctaactg ataatagcca
     7981 gcttgaaagc cagttaaatt tttaaatttt gtataataat tgaaaacaat cccgctttta
     8041 ataatattgt ccttctccaa aatctcacca atctgcttgt tagaagagcc actcgggatc
     8101 tcgacttgga ctaattggtc atctgattta tctaaaggct tcaacccgct atctacataa
     8161 cgatacacgg taaatccaag aaatccgcca ataatcaata atgtaagagc aatcactaaa
     8221 atgatcttcc tcacaatctt atcttctttt ttacgcagat ttctccgctt gtcgattata
     8281 tctgctgtag ctgccttact ggcttctttt ttatctttac ttgggtgttg tttgtttcct
     8341 gacaaaacaa ctcctcctta ctatcaagtt cgtaacttta ttcattatac atgatatttg
     8401 aattggcgtg aatgatttta tttaaatatt ctatcaaaaa tgtaagtagt aaatccatta
     8461 ctataagatt aaaataaatg ttatcaagat acaagctatt ttagctttat tctttctgta
     8521 aaaaagaaag gagactaaat aaactattat gaaaaaagga aaacatgcta aaaaaagaaa
     8581 atattcattt ttaatgatcg ttgtcgtgtt gtttagcctt agtgcgttct ctacaggaac
     8641 tattcttgga tggcaatttt acaataatga acactctcag atagttgccc atgctcaatc
     8701 agctcaaaaa aagttgatac tgactaccaa tgaacaacag ctcgttgaaa aaatcgatca
     8761 actattaaga aaaaatgatt atattggctc aatttacgta aagaaaaata atcgcatcat
     8821 catggaaaaa ggctatggct atgcgaataa actcacgcaa caaccaaatg atccttctct
     8881 ttattatcag attggttcgg ttcaaaaagc attgactact cttttgattt taaaacaagt
     8941 cgagcgtggg cacctttctt tagatactaa gctggctcaa ttctatccac aaattgctgg
     9001 cagccagcaa attactatcc gtgacttgct gtatatgcgc tcaggcttgc accgaactgc
     9061 ttctccaacc ataccgatga ctgatgaaga agtcgttcaa tttgcgattc agcacttaaa
     9121 atttacagat tatcagacct ctcattatga ttcattgaac tttaccatat tgactggaat
     9181 tttgatcaaa ttaacaaatc aaccgtatga aaaattattg accgatgata tcatcgaacc
     9241 tttaggatta cagcatacaa aattttatga ccaagtcaaa aattcatcca atcatgcact
     9301 atcctatcaa atgtctaaag aggatgatta ctttaaacca ctgacagaat ctgaaacaga
     9361 gatccataac gaattaggga ctggtaatat cagtatgtcc gtctatgatt taaatacgct
     9421 gtttacacaa gttttatctg gaaaactagt ttccaaagac ttactcttct cattatggcg
     9481 tgaaaatgcg aatggacatc catatagtgg tggggtatat agcggagata attatatcct
     9541 gtcgcaagga aatatcgact cttttgatac atctgctata tttagcaaag attcacagga
     9601 tgctatcatc atggaaagca atataaaagc tgacaaaact atcaaactgc ctactgctga
     9661 tctaagaaac caaatctatg acctgatgga aggaactact aagttggcaa atagctgata
     9721 tgatgaaaag atagcaaata ctacttcttt gaatagcatt tgcttaaaaa aagtactcga
     9781 ctaaacgtag gaaagagtgt gggacaaatc taaaaatcaa tttttgctac agactttaat
     9841 tccaaataaa cagaacagaa agaggtgttc ttctttctgt tctgtccaaa ttattctaag
     9901 atttctcttt accaacatta tttgacaaag ggcctattaa atctaaaata gtatctttag
     9961 attgctaaaa tatgtgctag caaatcattc atggcttgtt cttattttta attttatgca
    10021 ataaaaaaga agaacaatta gcgtgattat agaaataaat aatgagattt tagaaaaaaa
    10081 tgtgtatgaa tacctgattg ttactttatt tattccactt tcacttttta ctgtaacaaa
    10141 aggacctcct tgttttagta ctgtagcatt ttttgaatta acagtaaccg ttaatccttt
    10201 ataccggaat acaggtaaaa ctattgtttt gccttttgaa gatttaaacg taaatgtaat
    10261 ttcatcttta tttggagaaa agctactata aataacttga ccatcttctg taacttgatg
    10321 gtttttcaaa agcaattgat cttttttata gcctataggt acataatcaa aattctcaaa
    10381 ttcttcaagt ttattttcca tttgttcatt agaataagtt cc
//
LOCUS       DANKKG010000094        10415 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000094 DANKKG010000000
VERSION     DANKKG010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.094"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(208..1716)
                     /locus_tag="PW004_002012"
     CDS             complement(208..1716)
                     /locus_tag="PW004_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HDL2726973.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            1875..2066
                     /locus_tag="PW004_002013"
     CDS             1875..2066
                     /locus_tag="PW004_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726974.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            2269..3186
                     /locus_tag="PW004_002014"
     CDS             2269..3186
                     /locus_tag="PW004_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HDL2726975.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            3179..4252
                     /locus_tag="PW004_002015"
     CDS             3179..4252
                     /locus_tag="PW004_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726976.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            4400..4918
                     /locus_tag="PW004_002016"
     CDS             4400..4918
                     /locus_tag="PW004_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2726977.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            4899..5291
                     /locus_tag="PW004_002017"
     CDS             4899..5291
                     /locus_tag="PW004_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HDL2726978.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            5367..6302
                     /locus_tag="PW004_002018"
     CDS             5367..6302
                     /locus_tag="PW004_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HDL2726979.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            6435..7214
                     /locus_tag="PW004_002019"
     CDS             6435..7214
                     /locus_tag="PW004_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HDL2726980.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            7207..7524
                     /locus_tag="PW004_002020"
     CDS             7207..7524
                     /locus_tag="PW004_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDL2726981.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            7768..9378
                     /locus_tag="PW004_002021"
     CDS             7768..9378
                     /locus_tag="PW004_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDL2726982.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            9535..10383
                     /locus_tag="PW004_002022"
     CDS             9535..10383
                     /locus_tag="PW004_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDL2726983.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT         3290 a         1817 c         2089 g         3219 t
ORIGIN      
        1 tattttacct gttttgcaaa taaaatacta atctttagtg aaattttcat tactttttta
       61 gagtgaaacg cattcatttt ccattagaaa ataaagcgaa caaaataaaa ggtttaaaaa
      121 gtgtaacgtc ttagtaaagt ctataaaaaa acgaaactat cgattaagct aatagtttcg
      181 ttttcaacat ttataagtaa actttttcta ttggtaatat agattttcgg aagggtagac
      241 aggtgcgcag gtaatatcga ttcctagttt tttcaatgtt tgttcatcat ttttattgac
      301 catcacagta gagtgggctt gtgctccctc aagttcagct agtttatcat aagcaacttg
      361 agcgattgga ttcgttactg cactgattga taaagcgatc aagacttcat tgatgcttag
      421 tgcatccaaa gaggaatgaa gtgtctttga tttcaatcct tgtattgttt caagaactaa
      481 tggtgacaat aaaggaatgt catctgcgat atgggctaaa tgttttactg catttaagat
      541 aactgccgct gacgcatcca tcaatgaact tgtacgcccg gtaatgatga cttcatctgg
      601 taattcgatt gcaatcacag cagaaggatc ttttgtagga ctttgcgctt gaatcttatc
      661 tgcatagtca tgggcaggga ctaccacgtt tcggtcttca ggacgtaaac cgacttcttc
      721 cataatcagt ttgattcgat taactgcttc ttcgtcgatc aatccttttt tgaagtcgca
      781 ttctgtttgg aaataacgac ggatgatttc ttgtttagag gcttcttgga caacggtatc
      841 atccacgatc ccaaatccta cacgattcac acccatgtca gtaggagatt gataaacaga
      901 ttctttacca gtaatttttt caataatacg tttgattaca ggaaatgtct caacatcccg
      961 attatagttc acagctactt tattgtaggc atcaaagtgg aaggaatcga tcatattgac
     1021 atcttttaga tcgactgtgg ctgcttcata agcaatgttc agcggatgtt tcaacggaac
     1081 attccatact gggaaagttt cgaacttgga gtaaccagct gcatgtcctt tttggctttc
     1141 atgatacagt tggttcaagc aagtagcgag ctttccactt cctgggccag gagctgtcac
     1201 tacaacgatt ggtttagttg tttcaatata ctcatttttt ccaaaaccat cttctgagac
     1261 gattttttct acgttgactg gatagtcttc gatttctgag tgtttataga ccttgatgtt
     1321 tcgtctttct agtttattga taaagacttt cgtggcaggc tgtccactat agcgagtgat
     1381 aacgacactg ttgattaata agccataacc acgtaattca tcgattaacc gcaagatatc
     1441 catatcataa gtaatcccat aatcaccacg gatcttatta cgttcaatat ctccagcata
     1501 gacgcaaatc agtatttcag cttgatcttt caatttttgc agcaatttga ttttcgcatc
     1561 ttcttcaaat cctggaagta cacgtttcgc atgcttgtcg tcaactaact tcccaccaaa
     1621 ttctaaatat aatttgtcat agtggttcac tcgttccaaa atatacgctg actgctcttc
     1681 aatatatttt tctgaatcaa aaccaatttt tttcactagg ttccctcctt aagtactaaa
     1741 gcattttttc atttttttga ggattcgtca actggaagag cgaaattcct caaaaaaaca
     1801 aagaaaatgg tttaaaccaa cttgtctaga tagttattca ttggtatgat agtttttgat
     1861 aaggaaggtg ctacatgaaa aaaagacaga aaaagaaaaa cgcatataaa cactatatcc
     1921 gttcgatttt taccggttat gaaagaatgc ttgaagatcc cgaattggaa caattgacgt
     1981 ttacttatct aaacgaagaa acgcagctaa ctcgtgatga gcatcagcgc attcatttca
     2041 caacaagaga tctgcctagt aaataaaaaa taagataaag gtcatcggat tcgattctgt
     2101 cctatcagat ggaaagacta gttgatcgaa aaaaaggctg taccaaattt tggtgcagcc
     2161 tgttttatgg agcattgccc gctctttttt ataacaattt tttacagaaa atggtactat
     2221 tgaagtatta ataagtgaaa aacagatgag taagaggagg caagtagtat gaaagagcca
     2281 gatacaaatg caaattatga gccagacgaa ctagaggaga cgaacgcgga tcaggataca
     2341 ggacaagaat cgtcttcttt ttacgatatc aaagaaaaag caagcatgaa tctttcccaa
     2401 ttattagaag acttacaggc ttttgaaaaa gctgtccgcg aagaagatat ccctgagatc
     2461 taccgtttat acaatggccg attgaatgaa gaattaaaaa gaagctcaaa tgaaaatcac
     2521 gagatcgatc aattgctgat gcgtaagatc catgatcgtt ttttacagga atttcctttt
     2581 atggaacaag tagaaaacat ctcgccaacg atgagctatt acaaaatcgg cacttattac
     2641 catgatcggc ccacggtcgg tattgatgca agtctgccag aaatttttgt tttgcctaag
     2701 atcgatgaag agtgggagaa atattcccaa ccggagtcaa cggactatga caaaaaaatc
     2761 aatgagctag acgcaaaggt gatcactgct cagacagaaa ttgaacgttt agatgaacaa
     2821 ataaaagaaa tcaatcgaca aatgactgat ttgaacgaca ataaaggatt cttgaatcgt
     2881 aaaaaaatcg aggaagaaat tcaagaatta gagaagaaga agcaagtact atctaatgaa
     2941 aaattaggct ggcttcctta tattgagacg cctgaaacaa tccagcagca aaaagaagca
     3001 ttgaagcaag aagcaagagc agaccagctt cgtgcagcca tcgtagaaaa agaacaaagg
     3061 caaatcagac gctatttcgg tagcaaagag ggattcggtc aagcaatcca tgaattcttg
     3121 atgaattatc ttggcaatga gaatcctaat aatcaatcta atgaaggagg ttccgagtat
     3181 gaatgaacac gatgaagcag aatggacaaa aaatgaagaa caaacagaag atcatgaaga
     3241 aacgaacact tcattggaag aacaaacaag taatcctctg gatgacacag aagaaatccc
     3301 tatgctagag gaaacaaaaa tacatgaaaa agaaaaagta actccgacga atacgaatac
     3361 tgcagaaaac acccatgctt acgaaataac tgaaaatccc gggtatttag aaggaaaaga
     3421 gcaagatcat caagatatcg tcagtttgat tgaaacagcg gaagcagaat tagcggattt
     3481 acgtatgaga aaagatttga tcgaatcaga ttttccacaa ttattagaat catatgatcg
     3541 ttttatattg aatgaagaac caatctccac gatcggaaag acaagcctga tcgaatactt
     3601 cacgtatgaa ttactgaaac cgttgaatga tattggtctg gaacggtcaa attcagctta
     3661 tgacacatgg aaaacaagac atttttcgat caattatacg ttgaatggac aaaacgaatt
     3721 agtcttttcc tttgtattac cgaccatcga tcaacgatac caagtggaat cgatgagtct
     3781 cctaaacgta tcaccagaga caatggaaat caatgtccaa gatgaccggg tgctttcttt
     3841 gatccgacac tggtcagtgg atcgagtatt ctctgcaggg cagatcacga tattcaatca
     3901 caaattgaat caattattag cccatgcaag aacacttggt tttaccgtta atcagacatt
     3961 actagacaat acgaagccac ttcgtttgaa tcttcagagc gaatttgaat tgacgaatga
     4021 agtattggat gatattttta tcgtggcaat gaaaaatcca tcctacgata tggaaaagat
     4081 caacgaaact acttatcaag tacgattaga taaaggacag tctttaacga taacaacaaa
     4141 cgaaaaagat caaacagtct tgtcgatctc atctggagac tacccacgat ctgtcattga
     4201 tttctttatc aattacgaat tcttggtccc attaatggtg agaaagtcct gattggataa
     4261 gttggattac cagatgtcat tcagataatt ctgttccaaa ggacttgcgg cagttatcca
     4321 gaataattgt cacaagtctt ttctgtatgt actaaatgga cgtgaataac ttgctgatga
     4381 aggagggatt tcattaggaa tgtccacaaa taaaatagca cagcttagct tattgtctgc
     4441 tgcttgtgta gcaggtagga tcgcttttca atttattccg aatttccagc caatgactgc
     4501 tattttttta tttatagtac tttacttaag cttaaaagat gcgttaattg tcatgagtct
     4561 ttctattgcg atcagcagtt tttattttgg tgttggacca tgggtcatcg gccaatggat
     4621 cagctatact gttgttctga gtttgttttc aatcgtctgc ttgagaaaaa cagtgcaaag
     4681 gaatctatgg ttaaagggag tctgtttttt tctggcgggc atggtttacg gaataggtat
     4741 gaccgttttt gatacgctgt tatatcgttt gcctcaacct tggatctatt accttcaagg
     4801 ggtgtcgttt gatttgatgc acagtatagg caatgttgtg ttctttcttg tttttctgcc
     4861 agtcgtcaaa cgtttttaca atcattcagg aggaaaaaat gaaaaaaata atttatagtt
     4921 tgcttgcaat gagtgtcagt atactcattt tgacaagttg cagtacaacc tatacacaat
     4981 cggcttccaa ccaaaaggag gaagtaaaaa catcgcaaaa agcaacaatc acgttacaag
     5041 aaaatggtca aaacttttcg gaaaaagaag tggtcttcaa aaaaggcgat gatttactga
     5101 gtattttgaa acgaaatttt gaagtcaaag aagataacgg cttcatcact tcgcttgaag
     5161 ggcactcaca ggatgagaaa aatcaactct attggatgtt tactgtggac gggaaaagtg
     5221 ctactactgg tgccaaagag atcaaattga atgatggaca atcggtcatt ttcaatttgt
     5281 ctaaactata agtttcataa aaaactgtta aagggcgaat attgccataa ttcagtgagt
     5341 tcagttgatg aaagaaggaa aataacatgg aaaattttaa aaaaagcgga atgttttccg
     5401 taattgctgc tctaggggct aatattctag ttgctatcag caaatttgtc ggctatgcaa
     5461 tcagtggcag tgccgccatg ctaaacgaaa gcatccacag tatcgttgat tgcagcaatc
     5521 agattttgct gttattcggt gataaacaag ccaataaagg acagagcgag ttgcatcaat
     5581 ttggtgaagg acgagcaaaa tactttttca gtacaatcgt cgcaatgatg cttttcttcg
     5641 gcggtggggc acttggggtg atggaagctt ttgaaaagtt gacgcatcca tcccatgaag
     5701 tgggtaatcc gatcattgtt atagcaatac tgttatttgg tttggtaatc gaaggaagtt
     5761 cgttacgcgt ggcaatgaaa gaaatcaaag aattgaatac tgaaaaactt tccacgatgc
     5821 gctttttgcg cgaaagtcga cacagtgaaa tactgattat ttttacggaa gacttgtgtg
     5881 ctgtaatcgg tttggtcttg gctttagcag gaacaatatt gacttatgca accgggattg
     5941 ctgcatttga tgcgatcagc ggattgctga tcggtttctt gctgatggga gccgccattt
     6001 tcctagcaaa agaattttat agtctgatca tcggtgaaag cgtgacagca agcgatttat
     6061 caaaaatcaa gttagctttt gaacgtcctg agatcagtcg gctgatcgat ataaaaacag
     6121 ttcatttaag tccaacggag attttagttg cagcaaaagc agatatcgtt ggtaacaaag
     6181 agaatgaagc ttactcggtc atcaatgata ttgaaaaaaa tatgcgtcag tctcttcctg
     6241 acaagaagat gtatatatac attgaaacag ataaatatga cccaaattat tctcgtaaat
     6301 aaaacgttta ttcatgaatt gaagcaaaaa tgagaaaaat ataatcttgt ttttaaataa
     6361 aacggctaca atttagcgaa tttatgttac aatgagtccg ctgtttattt gttgtaatta
     6421 aaggagatat tttaatgaac aaaaagaaaa gtgcgccatg gcttcaagct ttgcatgcag
     6481 tgatgccact ttgtataagc tatataccag taggattagc ttgcggagta ttgttgcaaa
     6541 aagttggatt tgatcctctc ttatcaggac tgttatcgat tctggtcttt tccggtggcg
     6601 cgcagttttt agttgcatct atgcttacga cgcaagcatc atttgcaacg acattattga
     6661 tggttttctt tttagaatta cgctatacat tattaggagc aagtcttgcc ggttttatga
     6721 aaaaagaaaa gcggacgttt ttggctgtct tttctcaatc actgaatgat gaaaactatg
     6781 cggtgaacta cttaaagttt tcaactgacc cttcctggaa taaaagaaag gctttatatg
     6841 tcaattggtt ctcaatgact gcatgggccg gtagcaatat gatcggtaat tttttcggct
     6901 cggtgattcg agtagatgct gaccttgtcc attttgcttt gacggccatg ttcattttca
     6961 tgtttatcat gcagatgaaa agcgcactac tgatctttac aggtttattc tcaggcgtat
     7021 taggggtcgt attcatggta ttattccaaa atactttcgg attgattgca gcaacagtca
     7081 ttgcttcatt tgccggattt atgctggaga aggcattcaa aaaagaaaaa gctaagaaac
     7141 taagagaccg cggtaaagat gtcaatgagc cgctcttcaa cttcccggaa caggaggcca
     7201 atcaccatga ctaaactata tttgctactt ttagttgctt gtctgttcat cgtggcatat
     7261 atcccgcgct tgttcccgat gctttatttt actcatcgaa aagtgccatc ctggttcagt
     7321 gactggatga aatatgtacc ggtggcatta tttgcggcgt tagcttttaa agatgttttc
     7381 attactcatg aacatctgga tattgcttgg aatatcaaaa tagctgcaat gattctagta
     7441 gcaggcgtgg cttacaaaac acgttcgatg gcgttgtcag ttttgacagg acttgcgtct
     7501 gtctttttgc tgtctatgct ttaaactgtt ttattttatt aaaaacagag attgcgacat
     7561 aagttgttca aactgatcta tccgaactgc atttgcaaca gtcaattctg gatgcaagtt
     7621 tattcgtagg gataaaaaag attagtgttg gcttatgatg caagctcttt tttattttag
     7681 gaattagaga gggatttgtc cttctatgat tttttgctat aatagggaag atgtttattt
     7741 tatgaaagaa tagttgaggg aatttaaatg aataaaaaat tgatgcaagg aacattttgg
     7801 ctaacctttg ctaatcttct atgtaaagtt ttaggggtag tctatttgat tccctggcta
     7861 agtatgatgg ggaacaatca agatggtatg cttgctacga cattgtataa tgttggctat
     7921 cttccttatg gattgttcct gatgcttggt acagtcggat ttccaaatgc gattgcaaaa
     7981 aaagtagctg tagccacaaa agaaggagat aacgctgctt gtcggctgat ttttagaagt
     8041 acgatcaata tcatgtttgt catcggtatc gcctctgctg cactgatgta tttatttgct
     8101 cctttactag ccggaatcag tccgattgct aacgttaata atgggatact agctatcaga
     8161 agtttatgcc catctttgat tgctatccca attcttagtg caatgagagg atatttccaa
     8221 ggaaaaaatg atctgcggcc ttatggaact tctttgatta tcgaacaggc tgtgcgagtg
     8281 atcgttatct tggctggtac ttattacttg cgcgtattga ctgacggaac catattagaa
     8341 gctgtattga tcagtacagt cgcttctttc ttcggtgggt tagctgcgat tatccatatg
     8401 tatatcgtcg gacgcagaaa agacttcttc gaacttaaag attttttgat ttccagccgt
     8461 tatttcgaaa gagaaaaccg atctgcctct gtctctatca ttcgggaaac actaccgttt
     8521 atttttgtcg gctcagtcat tactatcgtt caaatgatcg accaagtaac gatgaaacca
     8581 ctgcttcact ttttccgtcc agagatcgca gatcagcaac tggaattctt attcagccgt
     8641 gcttctgtca atccaaataa attgacattg atcctgatct ccatggtagg aacagtagct
     8701 ataagcagct tgccaatatt aagcacgatg aagaaaaaag atcggataca aatcgaaaaa
     8761 acagtaggag atagtttttc gattgcgtta ttgattcttc ttccttcatt gactggaatg
     8821 tctttattag ctggaccact atacacattg ttttttggct atgatcctga aagtgtcgga
     8881 tatttccaaa tggctttgct agcttctttg tttttctctt tattcacgat cctatctacg
     8941 atgcttcaat ctttgaatca ccatcgtttt gcgatccgat taactgtaga agcaattatc
     9001 ctgaaagtca tcttccaagt aatcggatta ggattagttg gcggatacgg gatgagctta
     9061 tcaaatggct tagcattcgg tattgtcttt gttcgcggct atcactttat gtgcaaagaa
     9121 taccgaatag cacctttagc aaagatcagc aattttttct taaaaacttt ccgctccacc
     9181 ctgattatgc ttgcagtatg ttttgccgtt ttctttttgc tgaatcagcg attgtcaatg
     9241 gaatcaaaaa gctatgcaat gatttattgt gtggctgtag gaagtattgg cggcttagta
     9301 tttttatttt ctcaattcgg aaaaaatggg tttcgaatgt tgaaaaatgt taaacatcat
     9361 agcagaagca aagaataggt taactaatcg ttttttgctg tcacttgaaa aacggcaact
     9421 cgtatgatta cgcttgtcgt ttttctattt tccaatttta gtttcatatc taatcttact
     9481 tatgatatag ttataaggtc tggaaaaatt ctttgaaacg aggaaattaa acacatgaaa
     9541 cttgcagtag ttacagatag ttcggcatat ctgccggaac gtatcaagaa ccatcccgac
     9601 ttattcgtta ttccaatccc tttgattatg gatgggaaaa tttataacga aggaatcgat
     9661 atcgaagcgg atgaatatta tggactttta aaaaacagta aagattttcc tactacttca
     9721 caacctgtcg taggggaagt tttgggttta tatgaggaat tgaaagagaa agggtatgac
     9781 acgattttaa gtatccatct ttcttcaggg atttctggct ttgtcaacac attatttgcg
     9841 atgaaagatg acatcagcgg agtaacggtc attccttatg actctaagat cactagtatg
     9901 ccgatgggac acatggtaga agctgcctta gacttaaatg atgaaggtca gtcccttgat
     9961 gaaatacttg cacatatcga tcgaatccga gataatacct atgcttatct gattgtagat
    10021 gatttgaaca atttagtacg aggaggacgt ttgacaaatg gtgcagcgct gattggcagc
    10081 ttattaaaaa tcaaaccgat cctgacattt tctgaaggga aaattgtttt attcgaaaaa
    10141 atccgttcaa gtaaaaaagc atttgctcga gctgaagaaa ttattggtag aagaaataac
    10201 gaaatacaac cgccagtcaa actttacgtg atacatgcaa ataatttaga agtagcgatt
    10261 gcggagaaag agaaattgca gaaaaaatat ccaaatgcag aaatcgaaat tggtcatttc
    10321 ggaccagtca ttggtacaca tttaggtgaa aaagccatcg gcattgccat ttcagcgcaa
    10381 taattccttt tattcaaaaa acataccaaa aatat
//
LOCUS       DANKKG010000095        10400 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000095 DANKKG010000000
VERSION     DANKKG010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.095"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(18..263)
                     /locus_tag="PW004_002023"
     CDS             complement(18..263)
                     /locus_tag="PW004_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HDL2726984.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(377..955)
                     /locus_tag="PW004_002024"
     CDS             complement(377..955)
                     /locus_tag="PW004_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL2726985.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(1032..1562)
                     /locus_tag="PW004_002025"
     CDS             complement(1032..1562)
                     /locus_tag="PW004_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HDL2726986.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(1566..2426)
                     /gene="rlmB"
                     /locus_tag="PW004_002026"
     CDS             complement(1566..2426)
                     /gene="rlmB"
                     /locus_tag="PW004_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HDL2726987.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(2419..2814)
                     /locus_tag="PW004_002027"
     CDS             complement(2419..2814)
                     /locus_tag="PW004_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HDL2726988.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(2811..4223)
                     /locus_tag="PW004_002028"
     CDS             complement(2811..4223)
                     /locus_tag="PW004_002028"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HDL2726989.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(4220..4756)
                     /gene="cysE"
                     /locus_tag="PW004_002029"
     CDS             complement(4220..4756)
                     /gene="cysE"
                     /locus_tag="PW004_002029"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HDL2726990.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(4842..5081)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(5078..6538)
                     /locus_tag="PW004_002030"
     CDS             complement(5078..6538)
                     /locus_tag="PW004_002030"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HDL2726991.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(6708..7865)
                     /locus_tag="PW004_002031"
     CDS             complement(6708..7865)
                     /locus_tag="PW004_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HDL2726992.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(7899..9269)
                     /gene="radA"
                     /locus_tag="PW004_002032"
     CDS             complement(7899..9269)
                     /gene="radA"
                     /locus_tag="PW004_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HDL2726993.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(9332..9859)
                     /locus_tag="PW004_002033"
     CDS             complement(9332..9859)
                     /locus_tag="PW004_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HDL2726994.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            10269..>10400
                     /locus_tag="PW004_002034"
     CDS             10269..>10400
                     /locus_tag="PW004_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HDL2726995.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT         2654 a         2310 c         1879 g         3557 t
ORIGIN      
        1 tcaggctcgt ttgcttttta aactgcttca gttaaaaatt cgatctcaac tgtccgagta
       61 agtacatctg aatagctgta agatactcgt tcaaaagaat tttcttcagg atccaaatca
      121 acaacgaata ctgaaggata ggtttctgtt aaaacgccgc ggcgttctgt ttgacgcttt
      181 cttcctgtct gagcaaccaa catgattggg ctgccgatac gaccttctaa atctttcttg
      241 attgaagcta aagttgttgg catacagttc acctctacta tcaactatac cacatatttt
      301 taaaattttc aagtttacca taaaaaaaaa gaaatgcaat aaaaataaat aagaagctca
      361 ttaagatttt tctaaattat tcctcattat aaaatttcaa gtgttttttt attttgagtt
      421 ttactctccc gtaagcgctc attacttgct tgtgatccat tgctaaacat tcagccatgt
      481 ctacaaaatc aacgccttgc atataataat gaaaaatttt agcttctatt tttgaaaact
      541 ggatttcgca attttcgatt gcttctttta ataaaagatg ctgaatcgct aaaggttctt
      601 gcaatgtgat ctctgctaaa aagtctggtc cttctgtctc tattttttgg tcgattgaga
      661 cttcatcttt ttgatcacga cgcttcagcg cgttctgctt cctcaacaga gaatagattt
      721 gattgcttat aatgctttta taataacttc caaagctaac acctttgctt ttttcgtaag
      781 ttctcaatgc ctgatataaa gcgatccttg cttcttgttc ccaatcctca gaaacataat
      841 ttcttaaata ataggtgttt ttcaaacgta gtaaaagtgg aaaatatttt tgatacaggg
      901 cagcaaatgc ttcttcgtca ccattacgtg cagcgcgcaa taactcctca gacataaaat
      961 aattcctcca taaattcgtt tatggaggaa tcttatcatc aatgattcat cgaactgatt
     1021 agaacgatat gttatttatt ttttctcatt aaatcgtcca atttttggga caattttttt
     1081 aactgctctt cattccatgg agaattacgt cggaaatcaa taaaatgcat atctttattt
     1141 tcttgggcta ttgttttttc cgtttttttt atcgtcttat ataattcata agcagaagtt
     1201 cgtaatgctc cttgggaaaa gatcatccac tgttctgcta aatcgcttgt cgccactgtc
     1261 acttgtgtca aacggtcatt cagctgacca gcgattctct cgatataact gtcagccgtt
     1321 tcgtcttctt tcgtaaaaac aacatccaac tggtatttct tataattttg ttggatgccc
     1381 ggaactagtt gggcatcgaa gacaacgatg acttccattc cttcatattt cgcataattt
     1441 gataaacgat gtaaaagagc ttctcttgca tccgccattt tgtcctgctt tttaagctgg
     1501 acaagttccg gccacgaacc aatcatgttg tagccgtcta caatcaacag ctgttttttc
     1561 atggtctact ccccctgacg ttgggaaaaa gcctgataca taagcaaacc agcagcaaca
     1621 ctggcattta agctctggac gtgcccggtc atcgggatcg tcagcaattc atccatttct
     1681 tttttcaatc ctggactcat tcctttgcct tcattaccaa tgactaaagc aattgatcct
     1741 ttggcattcc atttttggaa gtttgtgccg gtcatatctg tgccaaatac ccaaaatcct
     1801 gtttctttga gctgttgcac agcttgtacc aaattcgtca cgcgtgcaac agggacatgt
     1861 tcgactgccc cagtggaagt ctttgttacg ataggtgtga ttccaactgc tcggtgtttt
     1921 ggtatgatca caccatcgac gccacttgcg tctgctgtcc gtaaaatcga cccaaaattg
     1981 tgtggatctt cgatgctatc caaaatcaaa aagaaggggt cttctttttt ctcttttgtt
     2041 cgttcaatca gttggtcaag ggataaatat tcataaggtg taatcgcaag aacgactcct
     2101 tgatgcacgc cgtgatcgct caaagtttct aatttttgtt taggaaccca tttgactggc
     2161 acagcttgtt cacgtgcgat ttctttcagt tcttctactt tttctcctcg tgaatcttct
     2221 tgaagaaaga gtttattccc tcgcccttgt tgtagcgctt ctactgcggc atggtgccca
     2281 aagacgaaat tatcttctaa tgcttcttca ttttgatcag aaactgactg atttcgtcca
     2341 gtttgctttt tatttgaata tctattttgt tttttgtctt caaatccacg tttcgattga
     2401 tgtttgtttg ttctttcttt acccattttt ttcaccaact ttctgaatac accagcggat
     2461 caattcttcc atccgctcgg tttgttttgt caaatgcaga tagcccatga gcgcttcgaa
     2521 ccctgttgcg acccgatacg tcgtaacatc agcatttttt gcgctcgtat ggcttttagc
     2581 atttcttcct ctgcgataat aaagttcttc ttcttctgtc aaaagatgtt cttccagcat
     2641 ttgctgaatc aaaaaggctt gtgcattcgc agaaacataa ttcgttgccg cacgatgcag
     2701 cttattgggt tttgtttctc cttgatcaat caggtggtca cgaacataga tttcgtaaat
     2761 cgcatctcca acataagcca aggctaaacc gttcaattgt gtgtaatctc tcatgcttct
     2821 cttctccatc gggttccttg cggtgtatct tctaataaga tccctttttc tttcaacaaa
     2881 tcacgaattt cgtcgcttcg gacaaaatca cgattgcttc ttgcttgatt tcgttcttcg
     2941 atcaattgtt caatctcttc atctaataac tcttctgctg taaataaaat accgaagatc
     3001 gaaagccatt gcgagaataa agcttctcct tgtgccaaga cagcggcaga aactttcggc
     3061 tggtctagat aagtgttcag ccacttagca agctcataga ccactgtgat cccatttgct
     3121 gcattaaaat catcgtccat ttcttcgatg aatcgctgtt ctaaattttc taacaaagcg
     3181 attttatccg catcatctgc caaattctct gaggcagttt cttggcggaa tttcaaattg
     3241 tcaaacgtat tcttcagtcg ttgtaaattg gtggctgctt ctttcatcgt cgtttcactg
     3301 tagcggatcg gacgacggta ttgtgtggtc gccatgaaga aacgcaagac ttgtggatca
     3361 acgtttttga tcatttcatg tacagtgatg aaattcccta gagatttact cattttttca
     3421 tcgtcttcgc caatcgttac atagccattg tgcatccagt aattagcgaa ttttttccct
     3481 gttttcgctt cactttgtgc gatttcattt tcatgatgag gaaattctaa atcttgcccg
     3541 ccaccatgga tatcgatcgt ttctcctaaa tgttttgttg ccataacgga acattcaata
     3601 tgccaaccgg gacgtccttt gccccatggt gaatcccaag aaatctcgcc aggttttgct
     3661 tgtttccata aagcgaaatc taatgggtct tctttttgct gctgttcttc ccctgtacgc
     3721 tgacttgctc cgacttccag ttcatcgatg gattggtcac ttaatttccc ataattttca
     3781 aatttacggg tacggtaata cacatcacct gaagattcat aagcatagcc tttttcaacc
     3841 aacacttgaa taaatgctaa aatatctggc atatgatcca tgactcttgg atgcagtgta
     3901 gcaggctcga cattcaacgc tttcgtatct tcttcaaatg ctcggatgaa acgatcagca
     3961 acttctggtg ctgtaatacc taattcgttt gctgtacgga tgattttatc gtctacatcc
     4021 gtgaaattcg atacgaaatc gactttatag cctcgatatt caaaatagcg tcgaattgta
     4081 tcgaaagcaa tggtgctacg agcatttccg atatggatat aattatacac agtcggtccg
     4141 cagacataca taccgacatg tcccttttgt atagggatga attcttcttt ttttctggtc
     4201 aatgtattgt agatttttat cattctgatc tcctcacttt ttctcctttg attcgaacga
     4261 cttttgctgg tactcctacc gctgtagcgt cgtctggaac atctttcaac acaacggcac
     4321 ctgcaccgat tttggcgttt tttccgatcg ttaccggtcc taatatctgc gcattcgcag
     4381 agagcatggc tccttgttta acagttgggt gtcttttacc cgtttctttt cctgtgccgc
     4441 ctaatgttac gccatggaat aaaatcacat cgtcctcgat ttctgcggtt tcgccaatga
     4501 cgattcccat tccatggtcg atgaacacgc cagttccaat cgttgctcct ggatggatct
     4561 caacaccagt taggaaacgc cagaattgag cattgatctt cgccaataaa aagagccggt
     4621 gctgatataa aaaatgagag aaacgatgcc aaaacaaggc atgaaaacca gggtatgtca
     4681 aaacgatctc agctgtcgaa cgtgcagccg gatcgtttct ctttgctgca tcaatcgctc
     4741 gggtaaacca actcatgact ttccactcct ttcgtaagaa ttttatgtag gaaatcatct
     4801 tcttataaag aaaagaagcg ccttttaaat aagtttcctt atcaaaagac gctttccgcg
     4861 tggttccact tttcttcata atcaatgatt atctctgaca gctaacgcct gttatacgtt
     4921 cttggctact ttgtttccta taaaacagtt tttcctgttt tctgtgtttc gccaagacca
     4981 ctcccagagg catttcggca accattcatt gataccttgc accacccggt atctctcttg
     5041 taatgatcag ctgcttactt ttttctgttc atcgtgttta gttcagtact tgatttaaat
     5101 gtgctaatga tttttcacga ccaagcaatt cgatcgtttc aggcaattct ggaccatgca
     5161 tttgtcctga tacagctaca cggattggca tgaaaagatt ttttcctttt acgccagttt
     5221 ctttttgaac agctttgatg gcagctttga cagttggagc atctactact tccatttgtt
     5281 ctagctgtgc tttgaatgcg tggagaacag taggaactgt ttcaccagca agtacttctt
     5341 ttgctgcttc attcaataca ggatgttcat tgaaaaatag cgtagacaga tcaacgattt
     5401 ctgcagcata gctcatttga ggttgataca ggctaacgat cttcttcacc cattcgattg
     5461 tcttttcatc tgggttttct gctacacgtc catcagcgat tagataaggc agacacatgt
     5521 cagtaaatgc atctagatcc attgctttga tgtaatggtt gtttacccat tgcagttttt
     5581 tcgcatcgaa agctgcgggt gatttactta ggcgttttgg atcgaacatt ttgatcaatt
     5641 cttggtgttc gaagatttcg tcttcgccca caggtgacca gcccaataat gcaataaagt
     5701 tgaacattgc atctggtaga tagcctaatt cgcgatattg ttcgataaat tgcaagattg
     5761 attcgtcacg tttgcttagt tttttacctg tttcactgtt gatgatcaat gtcatatgtc
     5821 cgaaggttgg tggtgtccag ccaaatgctt catagatcat cagttgtttt ggtgtattgg
     5881 caatatggtc atctccacgt aaaacgtgag tgattttcat caaatgatca tcgaccgcaa
     5941 cagcaaaatt ataagttggc ataccatcgc gtttttgtat gacaaagtcg ccaccgatat
     6001 tatcggattc gaacgaaata gcgcctttca ccatatctgt aaatgcgtat tctgtattgc
     6061 gaggcacacg gaaacgtaca acagattcta gaccttgtgc ttctttttct gcttgttctt
     6121 caggtgtcaa gttcgcacat gttcctgcgt aatgaggcat ttctcctctt gcgcgttgtg
     6181 cttcccgttc tgcttctaat tcttcttctg tgcaatagca tttgtatgct cgattgctag
     6241 caagcagctg atcaatcagc ggttgataga tttctttacg ttcagattga cggtaagggc
     6301 cgtattctcc aggattttca ggagattcat cccaattcat tcccaaccaa gcaaggtttt
     6361 ccaactggct tttttcgcca tcttcgatat tacgtttttg gtcagtatcc tcaatacgaa
     6421 taataaagtc tccatcattg tggcgggcaa acaaataatt gaataatgca gttcttgcat
     6481 ttccaatatg caggtgccca gttggacttg gtgcatagcg tacgcgtact ttcgtcattt
     6541 tcttcctctt ctttcaaact agactttgac taacagcaaa aattccgtca gttacggctt
     6601 ctcaaatatc atttatttaa agccactcag cacaaatttt accttttcta aggtaaatag
     6661 taaagccaac tggttgaatt tccttaccag ttggctgaat ttctttttta ttttgattgt
     6721 ttagtctttt tctctgtctc ttttttattg tcgattcctc gtcctgcatg tgcaggttta
     6781 gcaaagatca tgcgtccagc agctgtttga agggcacttg ttacaacgac ttcgatacgt
     6841 tcattcatgt aatgctgtcc atcttctacg acaaccatcg tgccgtcatc aaggtaggct
     6901 actccttgtt ggcgttctgt cccggctttt actactagaa cattcatcgt ttcaccaggg
     6961 atgacgactg gttttacggc atttgccaac gcattgatat taagaacagg tacattttgg
     7021 aattcagaga ctttattcaa gttatagtca ttggttacaa tgacaccatc taaaagctta
     7081 gccaatttga tcaatttgct gtctacttca ctgatatctt caaagtctcc atcgtacatt
     7141 tcgacagata taccctcttc tttttgcaag gcatttagga tatctaatcc gcgacgacca
     7201 cgcacacgtt tcaaactatc ccctgagtcc gcgatgtatt gtagttcgta aagaacaaaa
     7261 ttagggatca tcaaggttcc ttctaagaat ccagtctttg taatatcata aatccgaccg
     7321 tcaatgatca cgcttgtatc caagatttta tatttatgga aattttctcc tgattttcgt
     7381 tctaacagtt gactctcaac ttgactagct gtttcttgct cgattttttt gcttcgtgaa
     7441 ccaaaaactt tcttccattc ttcgattcgt gtcgttccca ttctcaatcc cagatagcct
     7501 aagatgatca tcagtaaaat cggcaataca ctattgacga atggaatttc catattgtaa
     7561 agaggggtcg agatcacaac acctaaagtc aatccgatga tcgcaccgat cgcaccgaag
     7621 agaagataag tcaagctgac ctcacttagt ttttgttcta cttgtttgat aatagctgag
     7681 atatgtttgg ccaacagcaa agataataaa aagaaaataa gtgcgcctaa caggctgttg
     7741 gtaaatgtat tattcagcca tccgtttgac tgttgaccga ttgcctgcca agctgagggt
     7801 aaaaatgaaa tacccaagct tgcgccgaca atgatcatca gcaaagtaat gacacgtttt
     7861 tgcatcgttt ttcctccgtt tcgacaatca tttattagtt agcggaacac acgcttcaac
     7921 gtttcagcca aagttgctac tccaactatc tcgattcctt ttggcgcttc ccagttacct
     7981 aagttgtttt taggtaggta tacttttgtg aagccgagtt tttggacttc tttgactcgt
     8041 tgttcaatat gactgacacg tctgatttct ccagtcagtc caatttcacc gatgaaacat
     8101 tccgttggct gtgttccttt ttctttatag cttgaagcaa tactgacagc gatagctaaa
     8161 tcaatcgctg gttcatttaa ttttacccca cctgcagctt ttaagtaagc atcttgattc
     8221 tgaagaagca acccagctct tttttccagc actgccatga tcagagatac ccgattgaaa
     8281 tcaagacctg ttgttgttcg tttggcattt ccgaacatag taggtgtgac taatgcttgg
     8341 atctctacca aaattggacg tgtcccttcc attgccacaa cgatagcaga gccagttgca
     8401 ccgtctaggc gttcttctaa gaaaatctgt gaaggattag cgacttcttc caatccatgc
     8461 tcatgcattt caaaaatccc aatctcattc gttgagccaa atcggttttt tactgctcgc
     8521 aaaatccgaa aactatgatg ttgttcacct tcaaaatata acaccgtatc caccatatgt
     8581 tctaacattc ttgggccggc gatcgagcct tctttggtaa catgaccgac gataaaaatg
     8641 gctatcccat ttgttttagc gattttgagt agttctgcag tggtttctct tacttggctg
     8701 acacttcctg ccacacttgt gatgtctggc tgcgtcatcg tttggatgga atcaatgata
     8761 acataatcag gggagatatg ttcgatggca cgactgattt cgttcatatc tgtttcagcg
     8821 tatagataaa attctgtgtt gatacttccg aggcgttccg cgcgtaattt tatctgctct
     8881 gcgctttctt cgccggatac atataagact tttccgccta tggctgctaa ttgctgagaa
     8941 acttgtaaca gcaaagtgga tttcccgata cctggatcgc caccaatcaa gaccatagac
     9001 cctggcacca cacctccgcc taacacacgg ttcagttctt ctaattttgt tttgatccga
     9061 ggttcttttt tagggacgac gtctgcgatt tttgttggtt ttgctttttc tcctgtcaaa
     9121 cttgtccgag ttctgcggtc cgttgtatct tgttcgattt cttcgaccat cgtattccat
     9181 tttccgcaat ttgggcagcg tcccaagaac ttaggcgaca catacccgca attttggcaa
     9241 acaaattgta cttttgattt ttttgccatt gtaccgcctc cttttatcct aatcatgaga
     9301 cattaaatca agcttaagta tctttaacgt tttatttact ggaagaacca aatccgcctt
     9361 gacgttgagc agtcgtttga tcttgatctg cttttaaaaa cggcaaaaag atcccttggc
     9421 cgatacgctc gccttttttg atcactacat ccttgaaacc aaagttggtg aattggaaca
     9481 tgatatggcc ttcatttgcc tcattattgt aataatcact gtcaatcaca cctactccgt
     9541 ttgtcaacac taaaaaccgc ttcaacggat tgcttgaacg gttagcaagc tgcaaaaatt
     9601 catcttctcc catgtaagct ttgattccag taggcacgag ggttggtttc aattgttttt
     9661 cattttctga tacatctttt ttcaatccat ctgcgactgt ttccaacccc tttttccagc
     9721 ctgctttcca gatacttgga atcacgacct cttctgctgc ttcaaaatca tagccagcag
     9781 catgatgtgt agcgcgctgc ggtagattga tattttgatc tttatattta gaaataactt
     9841 caaatcctct ttttctcacc ggttttcctc cctcgtaaaa tatcatttgt ctaatagttt
     9901 ttattcaaaa tgtttctaga ttataaaatt tattgaataa cacattccta acggaatttg
     9961 tttcgatatt gtgtgatttc tcagagtaca atggatgtat ggaaaggatc caacgggaat
    10021 ttgctatttc ctcctgtagc gtttgtctac cctgtttagt ctgttacccg aaccactgtt
    10081 atcactacca actcattagc tgtttcgata atggactaga ctttcaaata gtagtttgga
    10141 aggcagcttc tagctctttt atcaccgtat tctattaggc gaggttgtgg ctatacagtg
    10201 agtttctggg aaatcccaca gtatcgaagc attttccaaa atttatatcg aaaggaggtc
    10261 cttcttccat gaaattattt gtcggtattg acgttagctc tgaaaaacta gatgtttgtt
    10321 ttttaacaga cgacaatcaa ttgtctatct tatctgaaat ctctgttgcc aatgacatcg
    10381 aaggtgcttc attcatacga
//
LOCUS       DANKKG010000096        10106 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000096 DANKKG010000000
VERSION     DANKKG010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.096"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            266..472
                     /locus_tag="PW004_002035"
     CDS             266..472
                     /locus_tag="PW004_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HDL2726996.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(674..1675)
                     /locus_tag="PW004_002036"
     CDS             complement(674..1675)
                     /locus_tag="PW004_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL2726997.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1680..3593)
                     /locus_tag="PW004_002037"
     CDS             complement(1680..3593)
                     /locus_tag="PW004_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HDL2726998.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3761..4267)
                     /locus_tag="PW004_002038"
     CDS             complement(3761..4267)
                     /locus_tag="PW004_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL2726999.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4427..4867
                     /locus_tag="PW004_002039"
     CDS             4427..4867
                     /locus_tag="PW004_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HDL2727000.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(4893..6050)
                     /locus_tag="PW004_002040"
     CDS             complement(4893..6050)
                     /locus_tag="PW004_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2727001.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6053..6409)
                     /locus_tag="PW004_002041"
     CDS             complement(6053..6409)
                     /locus_tag="PW004_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727002.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(6707..7681)
                     /gene="sgrA"
                     /locus_tag="PW004_002042"
     CDS             complement(6707..7681)
                     /gene="sgrA"
                     /locus_tag="PW004_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HDL2727003.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSEISTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(7877..8716)
                     /locus_tag="PW004_002043"
     CDS             complement(7877..8716)
                     /locus_tag="PW004_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDL2727004.1"
                     /translation="MIRIIRLIGSVLIVFVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            8901..9788
                     /locus_tag="PW004_002044"
     CDS             8901..9788
                     /locus_tag="PW004_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDL2727005.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
BASE COUNT         3051 a         2020 c         1514 g         3521 t
ORIGIN      
        1 tttaggatac atcgaatttt tggggaaatt cttttggggg tatcctaaat cttttctatt
       61 atattcccat attttctatt attggtctag acgtatgatg gtaaactttg tacctatctg
      121 attgctgaat cattaattgc ttatcattag ccatctttgt ttcaagttcg ctagatgatc
      181 attttattca catcctaaca aacttgtcac atttatagat tcttaatcta tttatcatac
      241 aattataaaa aaggaggtgc tgcttatgaa agctatccat atttctttgc tttttttcgc
      301 tagtgcattg attttatttg ctattcatct ttgttcccag ccttatatca ataacaacgg
      361 aatattagtg gaaccagcat tcttttgctt accactcggc tttttgtccc ttcttgcctc
      421 cgcaggcttc ggatttgttg ctttttttaa acgatcaaaa caacaaatct gacttataaa
      481 atttacgtaa aagagatcgt gacaataatc gtgtgatatt cccaaatttc gaataaaagg
      541 tgccccttgc catctctaac tcctcagtca caagttacaa tattcataaa ccatgaaaag
      601 cttgtttatt gaaattgctc cttgtgactt ggagctaagt gacagattac aacctctttt
      661 gcatcataag actttactcc ttgaaaaaca ttactaactt ttgatatgtt tcttgcgctt
      721 gtttattgct gaaatcaaac tgatattcat ggttcaaaac agcatttttc tccgcataat
      781 ctacttttgt gactttcacc cccgctttct caaatacaga agttacttct tccatttgtt
      841 tcgtaaacgt atttgtattt ccatccgtta agaatgtgtt ttgcgtccaa cgatccgcat
      901 gtctgaccaa ggctaaactt ttgaccttct ctgattgttc aaagtcctta ttatcaaagt
      961 aagcccatcc acatttttca taaagaaagc tagagatttt atcacttact tgagtaagtg
     1021 cagaaggatc caacagacca gacaaagaaa taaacttcgt aatctgcttg ttctccaaag
     1081 tcggatcaag atgatcagct tttttaattt cttcattttg aagtgctaac aaatattctc
     1141 cactaatttg tgcacctgcc gaatttccac caatcgaaat attcgaccaa tctgcttgat
     1201 cttttatctc ttttttagct gcttgaatgg cttcgttcaa ttggataact ggtgtaggat
     1261 gttttgcttc cggtgatcgt tcataatcta tatttacgat gatttgctgc gtgtctgaac
     1321 atagatattc caaataatca cgacaatcat ttttatcccc tcctacatat gcgcctccat
     1381 gcatccagaa aagaatcggt aattttttct tttccacttt cggtgtgtag atatccatat
     1441 tattattttt gtactttgaa ggataggata tttctttttt tatatcgatt tgttcgattt
     1501 tttgctggaa atcatctggt cgggcatata cttgttcttt ttcacttgtg tcaaattgtt
     1561 ttctaacaag caagctaatc ggctttggtg tgatttgatt aatcaaaaat acagtgacta
     1621 aaataactac gatgataact ggtactgcaa tcactattcg cttgaaccat ttcattcatt
     1681 taccctctct ttcttcgcca aatccatgtg ataatcgtgc taacagataa aaatccacct
     1741 gaataaatga gaagcgaatg tttcttttct ccagttttcg gcaacattct tttttttgca
     1801 tctgtctttt tttgtacttt cactgtcatc tgaatcggtt gcgtatcatc acttgattga
     1861 tcctctcgag gttcattctt tcccttattg tctgcattct ctgtcggatc ttgctttttt
     1921 ggtggagcta ctgggatttt tttgtaatag aaaataatag ttggtatcgt ctcattaaaa
     1981 atcccttcag tcggtccttc aacatttgct aattcgtatc cttcgaatgt cttttgctct
     2041 gtcatataat tttctccata agctccgtaa agctggattt cgtcctggtc tttgagtaga
     2101 cttttattgt tttcatccac atactttacg tttatctgat catggaacac tactttcaaa
     2161 aagtccgtta catgtttcgt gtaggcagca tggtcaactt cctgtgcctt tccatgccct
     2221 gctccttcta ctaataaaag atctttatac cctcctgata actcatataa tttttgcgcg
     2281 ttagacacag gtactacatc atctgctgtt ccatggataa tcaatttagg caatgaatct
     2341 gacattccaa tcgtaggtag agcagctttt gtatccaaat gcatctcctg atcaaaatag
     2401 tattgtttca aaaggcccat aaataaatct tcatgctctt gatccccaat gatgtctaac
     2461 tgatttttta tggcatccgg tagtgcgggg gctactgtct ggctgtacgt aaaacgtagt
     2521 tgttcatcaa ttgatgcaaa tccgcaattt tcaataattc ctttgacatt gacaggaata
     2581 tctttcgcta atacagacat catcgtagct gctcccatcg aaccaccata aagtaaaatc
     2641 ttttgagatg gccgctctcg aacttcttga tttatccagt aaaggacgtc atctgactca
     2701 tattgtccat aggtcacata atcgcctccg ctattaccag tcgctctgga atcaacaaat
     2761 aataaattat acccggcctt ataaaaatca ttgtattctt ctgtaacgat cccgttattc
     2821 caattccctc gaaatccgcc atggatcatg actgttttat ctgaaccttg atcgacataa
     2881 tatgcatata catccatcat tttgccatct tgtttgttta gtacagtttg atgaagttgt
     2941 tttgtatttg gatagaagcc tgtgttttct gtaggcgttg tcccatcatc tgtacctcca
     3001 agcatcgaca tcaacacact gttttttgca aaagcgatat cgtacaatag ataagtagca
     3061 atcagatgtt tgataatctc tttatctaca ccataatcat taacagcaag ctcagtgact
     3121 aataactcta ttaattttga cttgatgtct tctgttggtc cattaagtat ttctgtcatc
     3181 tgtttaccga agtaattcaa atattcttta ccattgaaat tttcaccttt accgatatca
     3241 tcattttctg tcccattctc tttgtcactt actttagcag aagaggattc gtcgtttgac
     3301 atttttgtag ctgttgcttc ttctgtttgt tggttatctg tggtagcggt cgtttcttga
     3361 tctgtgctag taacagatgt ctcaaccgtt gtttcagtat ttgtttctgt ttgtgaagta
     3421 ctcgatttcg tttcatttcc agcatcagta gtttctgtgg tttctacctc ccgagattcc
     3481 gattcggaag aaatgctctc tccatatact gtccattgcg ggacgcttgt acttacagca
     3541 aataagatga ccaacgctcc ctttaatact tgttttgttc tcaagctatt cactcctttt
     3601 tatgtttcta ggagaattat atgtaaacgc aaacattttt tatacaaaaa ataaatggtt
     3661 tatcccataa aaatagaaca ggattatttt ctcattttac gtaataaagc agaatggata
     3721 aaccagtatc cattctgctt tttttaaacg aagaaacaac ttattgctcc aaaaatgtca
     3781 gaaatcgttt atcccaaatt ttctcgtaaa aagcgattgt ttcctttgct ttcataaaag
     3841 gattatcata agttgtcgtt gctcctctac acatcataag ctggtatcct aatccatgag
     3901 caaacttcac agtcgtatct atacaatact ctgcttgtgc cccacataat tcgatcgaat
     3961 gacagtccaa ttcagttaat aaaacactca aattagtctt ataaaatgaa ttcgcatggg
     4021 tttttctcac aaaataactg tgctcaggaa tagataattc cgacaatagc tcccatggtt
     4081 ttgtttgtgg cactaattga gcatcttcgt gttgaacgaa aataactggt ttcccaatcg
     4141 tttcgtatgt tttgattctt ttattcacta atgctgtcac tgtttctaat tcgtgtatcg
     4201 gcgaagaatc aaaacatacc cctttttgta aatcgatcac tactattgcg tcggctgtgt
     4261 ttgacatact tgtgccttct ttcttttttc ttgatttaat agtatttctt atctagctag
     4321 ttccattata cgtgacaaca ttttcttatt ttacacctct aaatggcact tttcgactat
     4381 ttcttcatgt tattatttca taaatactta gctattggag gaatgtatga tgattaaatt
     4441 gtatacaggt tctggctgct cgtcttcaag acgcgcaaaa cgttggctta tcgtccatga
     4501 tataccgttt atcgaacaga acttaattcg tcaaccacta accaaaaatg aatttatcca
     4561 cttactaaaa ttgacaaaca acggcgtgac cgaaatgctc tctactcatt ctattagtta
     4621 taaaaatctt gttcacagaa tagatgaact cagtttgagt gaactatata ttgagattcg
     4681 tacatcccct tctttattga aaaaaccgat tatctacgac caatctgcaa aacttttgac
     4741 tggatttagc gaagataaaa tcaggatgtt cctttctaaa actaaaagaa agcaagagct
     4801 caacgaactc ttgctttctg gtcatcttgt cgattttaaa gaaaataatc tttttgcaat
     4861 gtcttaattt tggcataaac aatctgcttt tcttaattaa cgaaatccaa tttttccaat
     4921 atggatctat ttttctcatt cctgattttt atgatttcag ctttcagacg cgcccaatca
     4981 ttttttgtag gttgtccatt ccagtaaaat atctgtgtat tcgtttgatc atatgccttt
     5041 ttgtcgatag gataatcgct aatgacaata tcaggtaact gatcagcgga tgcactatat
     5101 tttattgggc gttcagtcat agataatatc cttttctttg cccataattc ctgtgtttta
     5161 ccatactttg attgaatata gaatatgaca ggttgttcgt gttttataaa aatatccctg
     5221 actaacatac agtattgaaa aatcaaccgt ctgttctttt cataataagt attaagctct
     5281 ggaattctgc gagctacccc actaaaaaac tgttgcatga ttggatacat cactggaaat
     5341 atatgctgaa aatctctgtc ttctgctgat tttccaaaag catccacctt cttttttgtc
     5401 ccaaaaaccc ttgaatacaa atggatcatt gccaaattga catatagata atttttttct
     5461 gaagttgtaa aacaaatctt gaagtttttt tcaaaatttt ctacaataga cgattcaatc
     5521 cagccccaac tttcttgtac tttatctgga gtatccaaca aatcttcttc caaataggtg
     5581 ttcattacac caaacataaa ataaaggtaa gcttgctctg ttggagtgat gtgattcatc
     5641 gaccagaagt ttgcttcaaa aaaaggctct agtatgtaat gcgtaaactc ttcttttgtt
     5701 atcaatggat gcacaaattt gttcatctct tctgggacat acgtaatcga gtggccttgt
     5761 ttcattcgtt gcaacgaaat tcttagtagg ctgatccatt tctttctatc tatactacgc
     5821 gtatatggcg catgtttcgc aaatttttct aatagttgac gtttattttt tatacttgat
     5881 aatatatcag aatgattttc gtcgaatgac tgccaataca tcaagtgaaa aaagtatcgg
     5941 atacggtatt cttctccttc tatcggatta ccttttttca agctgcaaga taaatgatag
     6001 tttttcaaaa tagtattaag ttctcttaat tttctttgaa tcgtacgcaa acttatataa
     6061 ttttttgact aagattttct aaggattgat aagtaccttt gtaaagtgaa tccagtaaaa
     6121 tataaccaat cgttccctct aaaagtatag aataaacttt actcagtaaa aactgagaag
     6181 aaaactcgac ttttatcatc ttgttttctg aaagaaaaga taatttatat tctggatacc
     6241 tttcgcaaaa cgattctgct tgttgaatca ccgaattcat cttagccagg gtgtagttta
     6301 gatctaaagc gatatccttc accacatata atttctcgtc tctttcataa caatagttca
     6361 aaaattgata tacttgcctt tcattgatat caataaattc ttcaaacaaa gtacatactc
     6421 ctttttatct attatttaga cttattccga cagctacatg aataacgtac aaatagattc
     6481 gttactgatt tttcagagat actaccatgc gcagtgtgca ttgtcaatta ttcttgctct
     6541 tattttccat ttgtgttcct gttatttcct gtttctcaaa tatgttagta aacaaaaata
     6601 gatctggcaa agctacaact tcgccaggca atacagcact ctcgaatgaa atattcactc
     6661 aaaaatctat cgttttattc aattttagat ctgttgcttt tattctttat ttcactcgat
     6721 ggaagagaga acgaaaatag cttgctccta caatcaaaat gagtaacaca cccatccatg
     6781 taattagctt gttcaaggtt ctactaccag tttctggtaa atcgcttttt ctattatctg
     6841 tttctttcgc tttttcttga tgatcaattc cacgagaaac aaaatcaggc tgttttagca
     6901 cactttttag gttgctccct gcatttctct tttgttcctt agttggtatg ataggtacca
     6961 aatcaagttc attatttact tttcctgtgc tgctttcgct gctcgtgctt cctgtgctac
     7021 tctcactact catgcttcct gtgctacttt cactactcgt actctccgta ctgctttcgc
     7081 tgctcgtgct tcctgtgcta ctttcactac tcgtactctc cgtactgctt tcgctgctcg
     7141 tgttttctgt gctaatttca ctgctcgtac tctctgtact gctttcaccg ctcgtgtttt
     7201 ctgtactgct ttcactactt gtgctatccg tactgcttgg cggtccaaca actgtccaac
     7261 tgcttggttt caaattctcc gtgatcgtca tctgtccatc ataaaaaaca atcaaatcaa
     7321 ctttatcttt actcttgatc tcatcaatcg atagttctgc agaaatcatt attgtatact
     7381 caataccagc ggtccattgt acgtctttta aataaacttc cccaataact ttttctgaac
     7441 ttccattacc atcccaacca tatgttagaa cagcatgtac atcactagca ttcacgcttt
     7501 tgtttggttt gactggaata gtaatgactt gttgtgtcat gtcataaaca atccgatcag
     7561 ttttcacagt aaatgaaaga ttctcatttg cccgttcatt tgcagaagta ctgattccaa
     7621 tcattgctgc aattatactc agcccaagaa taacaaataa tacggtcgct gttttcttca
     7681 tttctatcac tctccatata attaccttct atttgataaa ggaattatat aaaatgaaaa
     7741 tgatgagaac acgacagtca gttgtgaaaa tctaacaaaa gatgtcgtgt attcgcttga
     7801 atttgtttac tttttcgttt aatcaatgac agacttaaaa aagcaagacc gatatcagcc
     7861 ttgctttctt catcttttat tgataaacag cagtgtattt cttcatccgc tttgttttat
     7921 atgcatagag taacaaacca acaatcaaga ctaacaagaa taatgtagtt gatatagatg
     7981 cttcagcccc aaagtttccc ccagtaatta ttgtattttg cgattggatc agagtggaga
     8041 aaaaaccttg ttcttgcgtt ccgctgactg gtatacccaa aatagctcct tgtgtccaat
     8101 tccaagtaat atgagcacca atagccgtaa ataaacttcc tgtactgtag ataagtgcgc
     8161 tgaaaaagca acctgctaaa aagatattga aaactgccag aaatgtcatg ttaggattca
     8221 atccgtgcaa aagagcaaat aatgcagcag atccaatcat tgctattaac ggaccaaatc
     8281 taagcatctg atgctgaaaa aagccacgga ataaaagctc ttctgatgcc cccactgcaa
     8341 taaataaaat aaaactcgtt atcagcggca caatgtttgc gtttcctgaa tctaaccgat
     8401 acgcccccgt caaatataat ggactagtaa taagcagaaa cactccaatt ccaacaacga
     8461 gacctcccgc taaatacccc cagtcttttc ctcgaacaga aacaccataa tcttctattc
     8521 tttttccttc gaccttacta gcaagaaata aagctagtaa aaaaacagct aaatgtccga
     8581 ccgtaccggc aattgctcca atccattcac tatttccaat aaacatatcc gcaagtgcat
     8641 tattgacaag aattcctaaa acaaaaccaa caaagactat taaaacagat ccaatcaacc
     8701 gaataatcct gatcatcttt catccctcct ttatattaag tttaacaacc cttttctttc
     8761 ttgtctcgtg aaaactatgt aaatgttctc catcatagag agaatagatt tttacgaact
     8821 aagaatatct acagcaacct tacgaaaatg aaagactgtc tccctaattc ttctgttaga
     8881 ctgtttttaa taaggaaagg atgggaaatt ttttgaaaaa acaactacta attatgtgcc
     8941 tttcagcatg tgcaatttta ctattctcag gttgtagcaa gtccaatgaa acgattgcaa
     9001 cgtttaaagg tggaacactt acagttcagg atttttatga taacccaaaa gttaaatcaa
     9061 ataatcaaac actattaaaa aaaatgattg tcttcaaagc atttgatgat gtatacggaa
     9121 aagagatttc ttcaaaggaa gtaacaaaag aatataatga acaaatcaaa aaactcggtc
     9181 caaactacaa ggaacaatta aaagcagttg gccaaacaga agagacatat aagaaactct
     9241 ttaaacagat gctggctttc caatacggtt tgaaagccaa tgtaaaatta acagataaag
     9301 atctagacac tgcttggaaa gaattctatc cagaagttag tacacagatc attctatttt
     9361 cgactaaaga agaagcagat aaagcaaaaa aagaagcaaa tgaaggagaa aatttctcca
     9421 agcttgttca agcttatgga aaaaataaaa cattaaagga aactgatgga aaaatgaatt
     9481 ttgattcaac caatccagag attcctactg aagtaaaaaa agcagcattt aaactaaaaa
     9541 acggggaagt ttctgatatc attcctgtaa cagatccaac gacttatcag caaagctatt
     9601 atctagtaaa aatggtaaaa aaacaggata aaggttctaa taaagacaaa tacaaatctg
     9661 agctagaaaa aattgctacc gaagcaaaaa ctcttgatac ggaattcatg gataaaacaa
     9721 tccgtaaagt gatgaaaaaa gacaatgtaa ctattaaaga tccttatgta aaaaatatat
     9781 tcaaataaac aaatttgacc caattgggga tgtggcaaaa tttgagaagt gatttttgga
     9841 aattccttct tatttttgag gcaaaccact tttgccacaa cttcatcggg gccttttttt
     9901 atttgtacca aaacaaatca ctggatttgt tacaatcatg aggatataat ttaaaaaagt
     9961 tttttatttc tatcacatga atcaatgcgc cattaagtag attctttcat gcttccctat
    10021 tttttcactt gccgtatcgc ttcaaaaaca actatcataa actcaaactc gactgaaaag
    10081 ttattttaaa aaagactaaa aatatc
//
LOCUS       DANKKG010000097        10083 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000097 DANKKG010000000
VERSION     DANKKG010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10083
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.097"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(109..2229)
                     /locus_tag="PW004_002045"
     CDS             complement(109..2229)
                     /locus_tag="PW004_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDL2727006.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2459..3637
                     /locus_tag="PW004_002046"
     CDS             2459..3637
                     /locus_tag="PW004_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HDL2727007.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3653..4411
                     /gene="aroD"
                     /locus_tag="PW004_002047"
     CDS             3653..4411
                     /gene="aroD"
                     /locus_tag="PW004_002047"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HDL2727008.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4434..4919
                     /gene="ybaK"
                     /locus_tag="PW004_002048"
     CDS             4434..4919
                     /gene="ybaK"
                     /locus_tag="PW004_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HDL2727009.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4990..6243)
                     /locus_tag="PW004_002049"
     CDS             complement(4990..6243)
                     /locus_tag="PW004_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL2727010.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6360..7709
                     /gene="gdhA"
                     /locus_tag="PW004_002050"
     CDS             6360..7709
                     /gene="gdhA"
                     /locus_tag="PW004_002050"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HDL2727011.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7821..9167
                     /locus_tag="PW004_002051"
     CDS             7821..9167
                     /locus_tag="PW004_002051"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HDL2727012.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9475..9861)
                     /locus_tag="PW004_002052"
     CDS             complement(9475..9861)
                     /locus_tag="PW004_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDL2727013.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(9910..>10083)
                     /locus_tag="PW004_002053"
     CDS             complement(9910..>10083)
                     /locus_tag="PW004_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HDL2727014.1"
                     /translation="TVETVFSSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQ
                     RRFEGTLRYSLGY"
BASE COUNT         3214 a         1839 c         2054 g         2976 t
ORIGIN      
        1 ctgagccaga atgaggttca ttctgaccca gatttgattt tgtacaaccc atcagtcctt
       61 attgacaaag agcctaattt tgtcgctgct ccttttaaat ttattttatc attcggaaga
      121 acttgtggtc gttccgtgaa gttcttggta cgtttgcgtt ttataaagat caacagttga
      181 ttgtccgttg tgttcactaa agacactagt tgacttgtca ccaagagatt tttcagcttc
      241 ttttaatttc tctaagccat tcttgtagtc ataatcactg gtatcaattg ggtctaatcc
      301 actattcgta tagaaccgaa gaagatcacc attattgatt tggtcagaaa cagctaactg
      361 tttatcaact ttttctttca gtgaatcaat ttcattctgt acttcctcag taggttcagt
      421 aatagcttca ccagtttcgt tactatagat cgtaccaccg tagtaagtat attttggtgt
      481 aacaaaatca ccatcacgga aagaaacaag ctgttcatga tctttcgaga atagatcttg
      541 tcctaactga atataatttt ttgtatctac acctagtaag tgaagtaatg taggcaaggc
      601 atcaacttgt ccgccgtaag tgtgattgat tccgccctgt gtttgtccag ggatatggat
      661 catataaggg acacgctgca tttgtgcatt gtcgtagttt gtccaagtag aacttgtttt
      721 tccaactaat tcagctagat ttttatttct tgaattcgag acaccgtaat ggtcaccgta
      781 aaggacgatg actgaattat catacaagcc gctttctttc aaataattga agaattcttg
      841 tacggcggta tctaaataat tggctgtcgc aaagtaaccg ttgatcgttt catcatttgt
      901 attggctaac gggaatcctg cttcgtcatt ggtgaatttt gaataaggat aatggttaga
      961 tacagcaatg aattttgaat aaaatggttg ttgcagacgt tccaagtatt gtacggattg
     1021 ttggaaaaat ggtttatcat gcaacccgta ttggaacgaa ttgtcatcat taacatcata
     1081 ataagaagaa tcaaagaaat aatcatatcc taaattcttg tatgtctcgt ttcgattcca
     1141 gaatgtacca gcgttcccat ggaataccgc actagtgtaa ccttgtgttt gacttaaaat
     1201 atcaggtgca gcttggaaag tattttttcc gcctaattgt gtgaacaagg agccttgatt
     1261 caagccaaac aatgaatttt ccatcagcgt ttccgcatcg cttgtttttc ctgcttttac
     1321 ttgatggaaa aagttgtcaa aactgaacgt gctgttgctg tggtaaagac tattgatgaa
     1381 aggtgtaact tcatgctgga gaccattctc atctgtcaat ttataatcga tcaggaattg
     1441 ctggaagctt tctaaatgga tataaatcac attttttcct ctagcgagcc cgaacaaatc
     1501 gtcatttggt gctgcataat gttcttttac ataattttct acttctttca tatcattggg
     1561 acttgcttct gcccgcactt ggtttgtctg gtaagtttga acaccgtcat aaaccatgaa
     1621 agcattcaag ccaagatatt tgaccaaata atcacgagag aatgttcgag tcagcaattc
     1681 tggacgatca gcttccgcca taaaaagatt tcctgagaaa atcataactg ctaatgtaga
     1741 gatcgcaaaa gccattcttg ctcggatcgg gcgagggtcc aacttgattt tttttgcaat
     1801 cagcagaaca gtcaagataa taaaatcgat ccaatagatc acatcatatg ggcgaaacag
     1861 tcgaatggca ctttctccca gaccactcgc tactttccca gcacctaaaa tcgtgttgat
     1921 cgtaatgaag tccgtgaatt ctcgataata agtgacattc gaaaaaacaa gcaatgtcaa
     1981 aagaaaataa atgaccatca tcgtgatata agcaactcta gggcgtctga tataaagagc
     2041 aattccaagt aataacaatg tagttgcaat agggttgata aacaagataa agtattgaat
     2101 gccactttcg attccgagat ggaaatcaac taaataggca aaaatcgttt tcaaccaaag
     2161 taaaacgact agtaaagaaa aaaatccgag cctcttattc aaaaaggcag gtgtttttat
     2221 acatttcaag ataatcttcc tttctgcact tcttaatcac agaagtgtcc tgaaaccatg
     2281 ttacacagct cacttattct catttatttt actagtatga caatatagtg tcaattgaaa
     2341 ccagaagttt atgctgtttc attaaggttt tctttaagct tcatgaaaaa atgagcagta
     2401 agacaagtcc tatgtttttt gttatacttg ggaagagaaa gctaaagcag gtgaaggaat
     2461 gaaagtaaaa gtaaaaaaaa gagcaagcaa tcgattgaaa aaaggatatc cactgatcca
     2521 aaaagaagac cttgctcatc cccaaaaaga aaccacgcaa tgggtggaat tagttgattc
     2581 gcaaaaccaa tttatcgcaa aaggatatct aggaaagcag aataagggaa tcggttgggt
     2641 attgagccag cagaacatcc catttgatca atcttttttt gaagaatttt ttgtgaagtc
     2701 aaaagaaaaa agaactttct ttttttcaga tgattctact accgcttttc gtatattcaa
     2761 tggagaagga gacgggatcg gcggtctgat tgttgattat tatgcaggtt atgctgtttt
     2821 ttcttggtat aacgaaacgt tatatgcaca tcgtgaatta ttgattcaag cttttcgtga
     2881 aatctacccg gaaatcatag gaggctatga gaaaattcga tttgaatcag taaatctgcc
     2941 tgagagccag ttcatttacg gaaaagaggc accggaaccc ttgatcgtaa aagaaaacgg
     3001 agtaaaattt gccgtttatt taaatgaagg attaatgact gggatctttt tagaccaaaa
     3061 agaagtacgg ggatcattag cagaaggttt cgcttctggg aaaacagttt tgaacatgtt
     3121 tagttatacc ggcgcctttt ctgttgctgc cgcaatggga ggggcaatcg aaacaacaag
     3181 tgtcgatcta gcaaaacgca gtttaaaaaa aacacaggaa cagtttgaag tgaatggttt
     3241 ggatgtcgat cagcaaaaaa tcattgttat ggacgtattc gagtatttca aatacgccaa
     3301 aagaaaacaa tttttatatg acatgattgt attggatcca ccaagttttg ccagaaataa
     3361 gaaaaaagtc tttcgcgtag caaaagatta tggggaactg gtcaaggata gtctggatat
     3421 tttagcagaa gacgggatac ttattgcgtc tacaaatgct gcgaatgtct cgatagaaca
     3481 attccaaaca atgattgaag aggaattcca tgccaaaaat gtttcttttg aacaacttgc
     3541 tttatatcgt ctgccaaaag atttccagac gacggatacc tttttagaag gcaattatct
     3601 gaaagtgttt gtttatcaaa ttaaaaataa ccattagaag gagcgatgca gaatggttcg
     3661 ggtaaaggga ttgaatatag gaagcggtag accaaagatt tgtgcgcctc ttatcggggc
     3721 cgtccgagag gaagtattac gtgaagcagt tcttgcacgt gaagcagggg ccgatttagt
     3781 cgaatggcga gtcgatcatt atcgtgaagt tttttctttt gatgaagtag cagaaacatt
     3841 ggttcttatc catgagatgc ttgatggact gccgttatta tttacgtttc gtaccttagc
     3901 tgaaggcgga gaacaagaca tatcgatccg tcagtatcgc gacctttatg aacatgtgat
     3961 acacacagga ttagtagata tggtcgatat cgagttgttc aaagtagaaa gtctaggaaa
     4021 aagactgata gaggaaatca agtctttaaa cattccttta attatcagta gtcatgattt
     4081 taaagaaaca ccagcagacc ctgtcttgct ttaccgattg aatgttatgg aacattttgg
     4141 agccgatatc ggaaaactgg cagtaactcc caataatgaa cgagatgttt tgcggttgat
     4201 ggagttgact agacgggcaa atgcttttgt ctccatgcct attattacga tgtccatggg
     4261 aaatcttggt aaaatttctc gtctggttgg cggaacgacg ggttccgtta tgacatttgg
     4321 ttccttaagc agcgattcta cttctgctcc aggacaatta ccggtcaaag cattgaagca
     4381 aattattcaa ttattaaatg aaccgttgta gaacaagttt gaggtgatat tttttggcaa
     4441 agaaaaaaat agtaaaaacg aatgcaatcc gtatggtcga acaaaaaaag attccctata
     4501 cagagcacga atatgagtgg gacgaaagcc acctaagtgc gtcatcggtc gctgaacagc
     4561 ttccagaaag ccaatctcgt atatttaaaa cactggttgc tgtgggcaat gtgacggggc
     4621 ctcttgttgc tgtgataccg ggagaagccg aattgaatct aaaaaaatta gcaaaagttt
     4681 cagggaataa aaaagtagaa atgctccatt taaaagattt ggaagcaacg accggataca
     4741 tacgtggtgg ctgctcgcct ataggtatga aaaaactatt tcctacttat ttagatcaaa
     4801 ttgcagaaag ctatgaacaa atcattgtct cggctggcag aagagggcta cagatggagt
     4861 tagcacctca agatatctgt gcattaacga gcggacaatt tgcagatata aaacaataaa
     4921 tcatacgtat aaaagaaaga aggaggccgg gatgttgttt gtccagtctc cttctttctt
     4981 ttttatacat tatgctcttt gcccggcatt cattcctgct acatgccctg tagcgaaagc
     5041 cgctgtgata ttaaagccgc cagtataacc attgacatcc agtaattctc ccgcaaaata
     5101 cagaccatca atgattttac tctccaatgt tttaggataa atttccttta gagaaacccc
     5161 tccgccagtt acaaatgatt tctcaatagg aaaagttttg ttgattgata gcttaaactc
     5221 tttgcatagt tgaacaaaat cttgaatttg tttttgagag gcttgttgtc ctgtcagatg
     5281 atccatttca agccgctcaa gaaagaaaac taacaatcgc tcaggcaata taccatgcca
     5341 agcattgacc aagttctttt tggttgcttt acttttttct gtcaatatat gaataagctc
     5401 ttgtttggtt tgtgtaggga aacaatccag agaaagtgtc actggttctc ccgttttctc
     5461 taattcttta ttgacaaaac ttgaacaacg cagtgcagcc ggaccagaaa taccgaaatg
     5521 ggtgaaaagt aaatccatcg tatgttcagt caatacacga ccttttttat ttaaaacgcg
     5581 taaagtgatg tcttgtaaag aaagcccttg aagtgttttc tcctgaatat acggttcatc
     5641 cgatatcaag ggggactctg tcgcataaag aggcgtcacc gtgtgaccca ctcgcttcac
     5701 gatcttgtac ccatctccag tagaaccagt agatggataa gtccgtccac cagttgttaa
     5761 gataatggca ggagcatgga actcttcgaa ttccgtgcgt ataccgtaaa ttttatgttc
     5821 tttgtggatc aatttctcta ctcgagtaga gaatagaagc gtcacatcta gttctttcag
     5881 acgattgagc aatgcttcga taatcgtttt tgatttattt gttacaggaa acatccgtcc
     5941 atgatcctct tcttttaaat gtacgccttg tgattcaaag aagttcatga tatcgaaatt
     6001 attccattgc gcaaacgtac tatataaaaa ctttccattt ccaggaatat gtgcaatcaa
     6061 atcatcaaca ggacgattgt tcgtaacatt gcaccgacca ccacctgtca tcagtagttt
     6121 ttttcctgct tttttgtttt tttcgatcaa gagtacgcgt gcgccttgtt cagctgcact
     6181 gatcgctgcc atcatcccgc ttgttccagc gccgactacg atcacatcat acagtttttc
     6241 cattccctca cctcaaggaa agtgtagcat atctgaagaa agaaaacaga aaaaaatgtt
     6301 ttgagccaaa agagaaaatg agctaaaatg agggaagata agcaaaggag cgtggaacga
     6361 tgacaaacgc aacagaatat gttcaagcaa ttcaagaaaa gctacatcaa aaggatcaag
     6421 ggcaagaaga atttttacaa gcaatcgatg aattcatgcc cacggttatg tcttttttgg
     6481 acactcatcc cgaatatatc gaaaagaata tcttagaact tttaacggaa ccagaaagag
     6541 ttattcaatt tcgagtgcct tggcaagatg atgaaggaaa ctggcgagta aatagagggt
     6601 accgtatcca atacaactca gctatcggcc cttataaagg tggattgcgc ttccatccta
     6661 gtgtcaatct aagtatcttg aaatttttgg cttttgaaca gattttcaag aatagtttaa
     6721 ccggtctgcc aatcggtgga ggaaaaggcg gaagtgactt cgatcctaaa ggaaaatctg
     6781 ataatgagat catgcgtttt tgtcaaagtt tcatgctgga attgtcaaaa catatcggcc
     6841 catcgattga tgttcctgcg ggagatatcg gtgtaggagc tagagaaatc ggctatttgt
     6901 tcggcgaata taaacgattg aaaaaatatg atactggtgt gttgacaggt aaacctttgg
     6961 atttttgggg aagtaagata cgtacggaag ctactggtta tggattagtg tattatgtca
     7021 aacatctatt gaacgaagaa aaagattcat ttaatcaaaa aacagttttt gtttccggaa
     7081 gcgggaatgt agccatttat gcaatcgaaa aagtgcaaga gctaggcggg aaagttgtta
     7141 catgttcaga ttctagcggg tacatctatg atccagaagg aatagatgtg aaactattga
     7201 aagaagtcaa agaaatcaat agacaacgtt tgacggaata cgcaaaccaa cgtccttctg
     7261 ctatttatca tgctggagaa tctgtttgga cattgaaaga aaaagcggat atcgctttgc
     7321 catgtgctac tcaaaatgaa atagacgaaa gcttggccga aatcttagta gaaaatggga
     7381 tcaaaatcgt agcagaaggc gctaatatgc catgtacagt aaaagctgtt tctgttcttc
     7441 atgaagcagg cgtgtggtat tgtccaggaa aagctgctaa tgctggagga gtagcagtat
     7501 ctgctttaga aatgagtcag aacgtccagc gattgacttg ggaaagagag caagtggatg
     7561 agcagctgga tcaaatcatg gaaacgattt accatacgtg tcgccaaaca gcagccgagt
     7621 acggcgacga aaaaaatctg cttcttggag caaatgttgc tggatttgaa aaagttgcca
     7681 aagcaatggc agcagaaggt ttagtttgaa aaatgaaact ttcttggaaa tattgtcagc
     7741 attttgttta tttgcttgaa aaataataaa aaaaaacgta aagtattacc tgtagagtaa
     7801 acctataagg aggagaacaa atggcacaca ttcattttga ctattcaaaa gtagcaccgt
     7861 ttgtcaacga acaagagtta gggtacatgc aaagccaagt aacagcagcc cacaacgaat
     7921 tacgggaagg tactggcgca ggaagcgact tccgcggctg gatcgattta ccaacgaact
     7981 atgacaaaga agaatttgca cgtatcaaag aagcagctaa aaaaatccaa tctgattctg
     8041 aagtattagt tgttatcggt atcggtggtt cttatttagg cgctcgtgca gcgatcgact
     8101 tcttgaatca cacattcttc aatttattag atagcaacac aagaaaagca ccacaaatct
     8161 tctttgccgg aaattcaatc agctcaacat atattgctga cttgatcgaa gtaatcggtg
     8221 atcgcgactt ttcagtaaac gttatttcaa aatctggaac aactactgaa ccagcgattg
     8281 cgttccgtgt attcaaagag cttcttgtta agaaatatgg acaagaagaa gcaaacaaac
     8341 gtatctacgc tacaacagat aaagcaaaag gtgcagttaa agtagaagca gatgcagaag
     8401 gttgggaaac tttcgtgatt cctgatgaca tcggcggacg tttctctgta ttaactgctg
     8461 ttggtttgtt accaatcgct gttagtggtg cagatatcga tgcattgatg caaggtgccg
     8521 ctgatgcaag caaagcatac tcaagcgaca aactagaaga aaacgaagct taccaatacg
     8581 cagcaatgcg caacattctt taccgtaaag gcaaagtaac tgagttgttg atcaactatg
     8641 aaccaggaat gcaatacttc tcagaatggt ggaaacaatt gtatggtgaa tctgaaggaa
     8701 aagaccaaaa aggcatctat ccttcaagcg caaacttctc aactgatttg cactctttag
     8761 gacaattcat ccaagaagga cgtcgcaata tttttgaaac agttgtgaag gttgaaaaac
     8821 ctcgtaaatc aatcacaatt cctgaacaag cagaagattt agacggtcta ggctacttgc
     8881 aaggaaaaga agtggacttc gtaaatacaa aagcatttga aggaacattg cttgctcata
     8941 cagacggtga cgtgcctaac ttgctagtca aaattccaga aatggatgct tatacattgg
     9001 gttatacaat gtacttcttt gaaattgctg taggtatttc aggttacttg aatggaatca
     9061 acccattcga ccaaccaggt gtagaagctt acaagaaaaa catgttcgca cttctaggca
     9121 aaccaggctt tgaagatctt gctaaagaat tgaacgaacg cctgtaagat aagggttttt
     9181 cgctagattt aaataatcag tgatagaaac atgttcgctt taaaatgaaa actggtaccc
     9241 atttaggtac tcaaacatac tgcatcgttt attcagaata aggggctgtg acaagacgtt
     9301 tgtcacagtc ccttgtttgt atagaaatcg attgattgcc ttgctcttta gtttgtttta
     9361 ggactttaag aacagtgagg ctgacttcga aggttaattc tgtttttttg ttttctagaa
     9421 tcagtaagca aaaaagcact cacttcgagt gcttttttgc gatatattct tagcttaaga
     9481 taggcctaat ttttctttta cagatgaggg taccttatcg ctggtaatta cttttttctc
     9541 tatgatccgc ttttcgcttg ctttcaactg caagatcgtt ccttcggggt aagcagaacc
     9601 attggcagag tcaccagaag taatgacatc gaaatctaac tgtctttttt caccgttttc
     9661 agcataagca tctacgttgt atttaaaacc tcttccaatt acttccccat tatctgcttt
     9721 gatatcagtt tctgcaggca ttggagcttt gactaccgca taataatctt tacctacata
     9781 agtagactga tagtagtccc aacctttcca tcctataaaa gcaatcaata tgataacgat
     9841 caaccctaaa atttttttca tgaaagatct ccttttttat ttttattata cccgtggtgc
     9901 tagttaattt taatatccta aagaatatct caaagttcct tcaaaacgtc gttgtgctcg
     9961 acttaataga acactgtaag ccagtaatat gctttcaaat ctactctcta gccctttaac
    10021 agaacgtgag ttgaaatttt gacaacctaa tttttctaag gaagaaaaaa cagtctcaac
    10081 tgt
//
LOCUS       DANKKG010000098         9952 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000098 DANKKG010000000
VERSION     DANKKG010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.098"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            255..734
                     /locus_tag="PW004_002054"
     CDS             255..734
                     /locus_tag="PW004_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727015.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            767..2995
                     /locus_tag="PW004_002055"
     CDS             767..2995
                     /locus_tag="PW004_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2727016.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            3124..4767
                     /locus_tag="PW004_002056"
     CDS             3124..4767
                     /locus_tag="PW004_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDL2727017.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(4840..5385)
                     /locus_tag="PW004_002057"
     CDS             complement(4840..5385)
                     /locus_tag="PW004_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HDL2727018.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(5408..5893)
                     /locus_tag="PW004_002058"
     CDS             complement(5408..5893)
                     /locus_tag="PW004_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL2727019.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(5883..6695)
                     /locus_tag="PW004_002059"
     CDS             complement(5883..6695)
                     /locus_tag="PW004_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HDL2727020.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(6834..8366)
                     /locus_tag="PW004_002060"
     CDS             complement(6834..8366)
                     /locus_tag="PW004_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HDL2727021.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(8475..9506)
                     /locus_tag="PW004_002061"
     CDS             complement(8475..9506)
                     /locus_tag="PW004_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2727022.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT         3318 a         1842 c         1915 g         2877 t
ORIGIN      
        1 tacaaaaaag atgttggtga agttcaccaa catcaaaaag tatagagcca aaatattcca
       61 acatcatctt ggactttcta catgttacta atgagcgtgt aaaagaacga gatggtgttt
      121 ttatttctat caatgaagct ctttttcttt gcaatcctca cttcaatcct ctataatgtc
      181 gcatatagtt tagtaaatga gtaaactact aatttactta tttactaaaa ataaaaggat
      241 gaggagttaa aataatggaa ataccaacaa atttaaaaca taaaccagta atcgttgtag
      301 aagactatga taaagttgat ggaagaaatg cattaaatat ggatgcaaaa gggctttcac
      361 tgggccttgc tcagtggaat gatcgtggaa aagtcgatat ctcaggcaag gtatggcgac
      421 atactggaga aaaatggtca cgacagtcgg aagaattacc aatcacacgt attttagatt
      481 tagcgatctt agctgcgcaa ggaagtttat acttccaaga tgcttatcgt tatgaaaaat
      541 tttatgatcc agaaaatcca gtcgtagaca tcattggatt acaagggaac cgaatgacgg
      601 tagaagtaga taccaaaaat ccaaagatcg atgaagacat cattttgtat tacgatacac
      661 tacaaaaaga cggagaactg attggggaac gattccgtat tttaaaacgt cttttagatg
      721 agctaggata ttaatagaca gagatgatca aaaggggaag agaagcatgg agaatttctc
      781 actaacagaa aaccgaacct tttcacggct agaaaagcaa atgatctgga agaaaccgaa
      841 aagccatcgg acaagtgcag aagaatggcg gatcgcctcc gaaatcaaag ggaattggca
      901 agatcctgaa atgaaaatca gtaacgtact tttggaagga gatgccgggt cggggaagac
      961 acaattagcc aaagcactat ctgctgatct gcaacttccc tatacaaaag tgacctgttt
     1021 tgcagatatg gacaaatcag atgttttcgg agccttatta cctgtagtag attctaataa
     1081 ggaacaagac caagaattac tagaagctat ttaccaaaca gatacacttg aagctgtact
     1141 gacactgatc cagaaccatt acggcattga tcctttttct gctaaagaaa aattagcaga
     1201 gctagttcaa aggatcgaag ctgattatcc agatgcaatc cattacagct attacccttc
     1261 tgaaatcgtt cgagcgattg aaaaaggcta tttattagag attcaagagc cgactgtcat
     1321 ccgagatgcc tctgttcttg tggcacttaa ctcagcgctc gaaccaaatg ggatgttgaa
     1381 tttaccgaca ggaatcattc gtcgccatcc agattgcgtc attgtgatca ctacaaatcg
     1441 aaattaccaa ggaaatcgcc cattaaatga atctttaaga gatcggatgc aacatgcgga
     1501 aaaaatggat cttcctgcct tagaagtcat ggcagaacgt gcgatggcga aaactatgat
     1561 ccaaaaacaa gaacttctct taaagatggc agagattatc cgtttgttgg atgaaactgc
     1621 aaaagctaac gccatcaaag gtgtagcagg aatgcgttct tatttttatt gggcaaatac
     1681 ctataagcaa ggacaagatt tactccagtc gatttatcca aaagttttgt ataaattatc
     1741 cactgattca gatgaattgg caattcttga acaagctttg tctgacagtg gattgctact
     1801 ggaacttcag gacatattga gaaaagaaaa atggggtcat cctcaatctg agcatacaaa
     1861 agaaagaacc attagcgccg aagaagcaaa tgaacgaaac atcactgaaa atgctgaaga
     1921 aattctgcgc ttagatgaaa agaaatcgtt gtctgctgaa gaaccaaatg agggagaaga
     1981 gaaacaagaa gaaacaatag aaaaaaacga gattcctcct caaatgcaaa aagaacgcag
     2041 tagcgatatc gaaggaaaaa atcaaaaaga acaacaaaca ggagatgatt ctcaagaaat
     2101 gtctgcttct gacatggaag cacatgataa ggaaatcaag aagcaactga ataaagaagc
     2161 gcgacaaatc atgaaggaaa cgattcatga aaaagaaggt ctcatcgttc accgaccagc
     2221 tttccaagga aaaacgccgg aagcacgtga gctacacatg caagtaatgc caatagtaga
     2281 atcacttagc cggcaaattt tagagttact ggataatgaa caaacagaga cgtaccaaaa
     2341 aggaaaatac gaaggtcagc ggttcaatgc aagcaaagta gcatatggtg atttgagaaa
     2401 ttttgacaaa aagaacccgc cacatgagca accttcttta gcggtagctg tacggatcga
     2461 tgaatctggg tcaatgatac gggatgatcg gatcgaagcg gcaaaaaaag cagcgattgc
     2521 cattgccgaa ttttctaaga aagtcgatat cccattgttg atttacggag atacagcaga
     2581 ccgttcgtca agagaaaaga cttctttgtt ttcttataaa gaatttgaag acggatttca
     2641 atggttaaat gaaaaattag tcactatgaa accaaggcaa aacaatcgtg atggtgctgc
     2701 acttcgttta gcagcagaaa aattaaacag acaatccgca acaaccaaac tgttgcttaa
     2761 tattagtgac ggtcagccaa aagcgttgcc ggattataca ggcaaaaaag caaaagaaga
     2821 tatccagtct gttttgaaag aatatgaacg tcaaggaatc ctatttgtct ccgcagctat
     2881 tggccaagac aaagaagaaa tcaagtcgat ctacggtgaa agtcgttttg tagatattac
     2941 tgacttgaat gaatttccca agcaaatgat tcaactgatt gcccgctatc tatgataaaa
     3001 taaagctcaa cgaacgcact tagcagattc gttgagcttt tcgtttctag ttaccggtaa
     3061 caaggatact tctaaaatag aatcggttat aatcaagagg gaccgttaaa ggagtgaaac
     3121 gatatgatac aagaacaaaa atggtggaaa gaagcagtag gctatcaaat ctatcctgct
     3181 agtttcaaag atagcaatgg cgatggtcaa ggagacatca atgggatccg tgaaaaactt
     3241 aattatttga aagatcttgg tatcggcttt atctggatca atccgatcta tcaatctcca
     3301 tttgtcgata atggttatga cgtaagtgac tatcaagata tagaagagag attcggaacg
     3361 atggaagatt ttgatttatt gctaaaagaa gcacatgaat tagggatcaa agtgattatg
     3421 gatcttgtca tcaaccatag ttcggatcag catcaatggt ttgaagaatc gaaaaaatct
     3481 aaagaaagcc cgtatcgcga ttattacatt tgggtcgacg gggtagatgg caaagaaccg
     3541 aataattgga catcgatttt cggcggttct gcttgggagt attctcatga aactggacag
     3601 tattatctgc atgtttttgc aaaagaacaa ccagacttaa attgggaaag cgagaaattg
     3661 aaagaagaac tattcaatat gatccgatgg tggttggata aagggataga tggatttcgt
     3721 ttagatgcga tttctcatgt aaaaaaagat gaatattcag taaaagccac agagaaccct
     3781 ttctcaccct ttcaaaatgt ctcaggaatc gaagaacatt tgaccgattt gaaacatgtt
     3841 tttgaagagt acgatatcat gactgtcgga gaagcaagtg gtgtcactgc agaagaagga
     3901 ccacaatggg tagggaaaga cggttatttc gatatgatat tcgaatttga tcatatccat
     3961 atctggcagc aagaaaaaga agggcaatta gatgtcctta aattgaaaca tgcattaagc
     4021 gcttggcaaa cttcattgga tggtatcgga tggaacgctt tatatatgga aaaccatgat
     4081 gttcctcgtg ccgtttcagt gtttggagat actcgtcccg atttttgggc tatgtctgca
     4141 aaagcaatcg ctatgatgta tttctttttg caaggtacgc catttattta tcaaggacag
     4201 gaaatcggaa tgacgaatat gccctttgaa tcgatcgatc aagttgatgc agtagattcg
     4261 aaaagacttt ataaaaggct gcttgcggaa ggtaagacaa gagaagaagc attggatatc
     4321 attcgtgaaa caacgagaga caacagcagg acacccatgc aatggacttc ggaacaatat
     4381 gctggttttt ctacacatga accttggctg atcactaatc caaataccaa aacgatcaat
     4441 gtagaacaac aagaatatga accagagtcg gtcctgcaat tctataagaa catgatccgc
     4501 attagacaaa ccaataaagg tctgatatac ggttcttata aagaatatct tcatgaacat
     4561 ccacaacttt atgtctatga aagatattta gaagatgaac aatatcttat tatggtaaac
     4621 ttaacagaat cacttgctga ctatgaactt ccaaaagaag cagatcagtc ctggacatta
     4681 ctcttgtcaa attcctctag cggagaattt gaagctaaag gcatactagc accttatgaa
     4741 gctcggttat ataaaacaaa caagtaaaac ttcctcgtta aagttcatta gttggacttt
     4801 gcgaggaagt ttttcagatt gctctgtcaa ttactcattt caataattcg ctctaggacc
     4861 accgatatat ttaggacgag agcgaagggc tgtcacgtga gcttgtccaa gggttttcaa
     4921 acgaggtctg acaggtactt ggacatgctt gatatgcata ccgattgatg tgtcaccgat
     4981 atcgataccg gcttgtccaa ctacgtgttc cacttcgaca ggatcattga actggtcaaa
     5041 agcagcaaca gaacaagcac ctccagcatg tagtgcagga acaacagaga caatttcgaa
     5101 attttttgct tctgccactg ttcgttctac tactaatgct cggttcaaat gttcacaacc
     5161 ttggacagct agcgcaattt ctttaggatc caacaactct tttaacgtac ggatgatcca
     5221 ttgaccaact tccgcactgg aattttttcc aatatgtcct ccaacgactt cgcttgtgct
     5281 gcatcctaaa acaaaaagat cacctttttt caagtgtgcc tcagccaata tgtcattaac
     5341 tatttcagtc aattggtctt ttaattcttt ttctgacaac ttcattttct aaaaccttct
     5401 ttttcaatta atattgcttc cgtgcttgaa tacgttgaag acttgcagtt ataggaaagg
     5461 caagtcccat tccaatcaga ttttggaaga tgtttcctgg tatagaagcc actccagccg
     5521 gccaatcata taaaatccaa ccggcgatac catagccgat gatcataact aagcttccta
     5581 ataaaagccc taatccccac atacgagtag aagatttgct gaaagaacca gcaacatatc
     5641 cttgtaaccc gtggatcact aatgaaaaaa ctgcccactg cggatatcct gaaatcaaat
     5701 cgatcaatag accactggaa gcaccaacaa ctaatccacc cgttggacca taaagaaaag
     5761 cagcggtata gatcccggcc tcgcataatg tcacaaatcc attcgtagcc ggtacaggga
     5821 tcaaaattgt taatgaaagg gcaactgtca acgcaatcag catagcatag atcgttaatt
     5881 gcttagtttt catttctcat tcctcctttt tgaataccgc cagaccagac acttccggta
     5941 tgatcgttca tcagcacgcc attagaaata caatgggcaa caaatttttt actttctgct
     6001 actgctttgg taaaatctgc ccctaacaca agattcgctg ttattgcaga agaaaaggca
     6061 caaccggccc cgtttactgt tgtagtgtcg atttttttat gagaaaatac ctcataatga
     6121 tcaattgaat agagaagatc cacagcttca tctccagcca tccttgtgcc gcctttgatc
     6181 acaatgtttt ttgttcctga agcagtgagt tctttagcga gatggatcat ttctgttaac
     6241 gaagagggaa gagcttgacc agaaagtaaa gcagcttctg ccaaattagg cgtaatcatc
     6301 gtaacaagtg gaaaaagttc ttcgatgagc tgcgaaatat acaattgatt gatcacagct
     6361 tcagtttctt tgaatgccaa aacagggtct aaaatgattg ggatattttg ttttgttttt
     6421 atgaagtctc gaacaaccat gattgcttca cttgaattca ataacccaat tttgatacca
     6481 tctaatgaag tcattttatc aatcgaaact aactgtcgtt taagcaattc caaagatata
     6541 gattccagca taaatcgctc attaatatct agtgccccta tagaagtcaa ggcgcttagt
     6601 ccgaatagac caaaattctc aaaagttttt aaatctgttt ggattcctcc accagcaaat
     6661 ggatcagaac cgccaattgt caagattctt ttcataacat tttcctccat gaataagagt
     6721 ataacgaaat tttcaaaaaa gaaaaacagc caattggcat atttttatct catccaattt
     6781 aaaaaaacaa aaagaaaaga aagaccaaaa tggatctttc tttccttaaa tagttaggac
     6841 ttttgtgatt ttaattcttt ctcgatttga agccgttgca ctttcattgt agctgttcgc
     6901 gggattgcat caaaatcacg gatgataggg acgttcaagc gaggaagttc atggacttgc
     6961 tcccaccatc gattccaatc catttcttta tcaggagcta aagcaataat cggttgtgga
     7021 gaatgatttt tatcccttac aatcactact tctgccaaaa aatctaacga atctaataaa
     7081 aagtcttcaa tagctaatgt actattgatc gtttcgatca aatccacttg ccgatctttc
     7141 aaaaataggt ggccatgctt atccataaaa ccgtaatctc cgctatccca ccaatcgcca
     7201 taaacatttt cctggaaccg tgcatcttcc ttgtaataag tcaaagcccg tcctttagac
     7261 agtaattgta tatgaccatc tgtgttttca ggtaggacat tcccaactga atctgtaatc
     7321 cgagcgcttg tcatgtcttc cagtccaacg cccatatctc ttgcatcact tgttttcaaa
     7381 ctctctagtg tatgagcctt aagaatcatt ggtccgcatt cactttggcc atatacttga
     7441 agaaataccg catcctgtgc catcgacgtt cgcaaaaatg tcttcattgt ctggttgttt
     7501 atagcatcaa aagtggagtg atagtatcgg atacccgaaa aagcttctgg atgctcctta
     7561 gcagtaaaac gccactgaac aaagttattc ggatgggtct ctagtgcaat tggccggtga
     7621 gcttccaaca tatgaacaac ttttgaactt tgtgcgtttg ctaaaggcat cataggaaat
     7681 cccatggaca tcaaagaaga gataccaata ttgaatctgg aatgcactgg agagatgtga
     7741 aaagcgatta atttcttttc cgaaattttt gtgaaaactg ttttttgcca tttcgtccgc
     7801 catcccatag aatgagcaga atgacaaata agtttaggga taccagttgt accagaagta
     7861 tgggtcatat aagaaatttc atttttgtct aaaaatgatt gagaaacaga caaagcaggt
     7921 tgttccatta aataaagaat aggaatctgt tttgattttg ttccatttga aatttctctc
     7981 actcgctttt ttgtctcatc atcaaataaa atgaaagggt cttctaaacg atcgataaat
     8041 acttccattg ttgttgttgg tagatgataa gaagtcatga caggcactgc accaaggtaa
     8101 gacacagaaa cagctagaag gtatgtgtca aaagcggaac tcttataaat aataactttg
     8161 tctccggatt tcactccaag atgagccaat tgataagccc gtttcaacac actttcgcag
     8221 cattttttgt aagttgtttc aagtcctaat tctgggaaag ctgaaagtgg ctcatcaaag
     8281 ataatcgggg tctcggaaaa gttagctgca gcttcttgaa aattcgtaaa taagttcaaa
     8341 ggggaatacg ttatggtctc catcattcac actccattat cttttgttaa gtaatataca
     8401 aattcaagat aatcgtaaca taatccttta tataataaca tagtttattg tgaaaaaaag
     8461 atcatacatt tgttttatat ggaagaaacc cgaacgaatg ccgctaattc gtgataagaa
     8521 aaatcaacca tcttttccca gatatcttga aaagaatggg atgagaggta tcgattacca
     8581 cataaataat ttaccgcgtc agcatcatct aaatatagtt ctgcataata acgatatgtt
     8641 tttctgcata gttctggcga tacgttttta tatactgtaa ttttgctcaa aatttggcga
     8701 atagtccgag gagaaatttg ctgatcatgt gaggaacgaa aaacaaaaga attttcgtcc
     8761 gtattttgtg ctgttaactt aaaatagttc tccagaaatg gcatcgcttc ttttggtatt
     8821 ggtactttat tcagtctgtt ttcttttcta ttcagattaa gaatctgctt ggtcaaagat
     8881 acatctttac gtttaagttc tactacttca tggatcttta atccgcaata cagaagaatg
     8941 gcagtaattg caaggttcct cggtctattc ttttcttcaa agctagcttc tctgcttgtt
     9001 tgaaagcctt caatcgggat aaacaaatgc tcaaaatctt tgaaaagttc tgcatcgata
     9061 tatcgcggag ctgctttggt tgtttccggt aaacttgtac ccaaaagaga aatctcttct
     9121 tcaatcagtc ctttttgata caaaaatcgt aaaaaatgat ttaatgaata ttgcttcctt
     9181 gaaatcccgt aatagctatt ttcaaacaat tgctccgttt cttggttcgc tgtagtttgg
     9241 aacgaacgag tatgtcgata gattttagct aagaaattct gataatcttg tttttctaaa
     9301 tggataatca gctgatcatc cacttcttca gaaaatactt ttctcaaata atcagtaaac
     9361 agatgaaaat cttttgcgta ttcatagcga ctgcctaatg aaagggaaga acgattttct
     9421 tctaaaaact gttgaaagac ggaagggagc aagtgtgtac gttgctttaa cttttcttgc
     9481 agttgttttc tagttttgct gtccattaca aattccctcc aatagtgttc attttctata
     9541 ttttaccgca tttcatcaaa tgaagataga cagtttgtct tgatcttagg aagcaaattt
     9601 ttaactaagt actagctaaa agatatgaag taagaagata catttttagt tattttatta
     9661 gtgcagtcat tcttttgatc ttttaattga tttatcctct ttttagctat gtggttactt
     9721 catgagcaat gaaatggatt tggttcgttc taatggcagg aggctctttc ttttatttcc
     9781 gaaaaattag aaaacatttt ttcagaatcg attggctctc catttattca ataatggttt
     9841 gtctaacgat attgttggga taattcttaa agaagtatta gaaatagttg gtacttcatt
     9901 actttacatg aagatttatt ctatggtagg agcaattata ttgagaatta at
//
LOCUS       DANKKG010000099         9873 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000099 DANKKG010000000
VERSION     DANKKG010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.099"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            163..1134
                     /locus_tag="PW004_002062"
     CDS             163..1134
                     /locus_tag="PW004_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HDL2727023.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            1158..3350
                     /locus_tag="PW004_002063"
     CDS             1158..3350
                     /locus_tag="PW004_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HDL2727024.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNDKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            3367..3843
                     /gene="ybeY"
                     /locus_tag="PW004_002064"
     CDS             3367..3843
                     /gene="ybeY"
                     /locus_tag="PW004_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HDL2727025.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            3827..4222
                     /locus_tag="PW004_002065"
     CDS             3827..4222
                     /locus_tag="PW004_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HDL2727026.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            4237..5136
                     /gene="era"
                     /locus_tag="PW004_002066"
     CDS             4237..5136
                     /gene="era"
                     /locus_tag="PW004_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HDL2727027.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            5279..6091
                     /gene="recO"
                     /locus_tag="PW004_002067"
     CDS             5279..6091
                     /gene="recO"
                     /locus_tag="PW004_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HDL2727028.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            6475..7392
                     /gene="glyQ"
                     /locus_tag="PW004_002068"
     CDS             6475..7392
                     /gene="glyQ"
                     /locus_tag="PW004_002068"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HDL2727029.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            7394..9469
                     /locus_tag="PW004_002069"
     CDS             7394..9469
                     /locus_tag="PW004_002069"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HDL2727030.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKKSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT         3314 a         1762 c         2129 g         2668 t
ORIGIN      
        1 atcaaaacga gacctttagc gaatggaaac agctattttt taacgtttct tggtacgtat
       61 ggttaaacga ctcttttaca accttttctt atttgctata tagtttcttc tgagtatggt
      121 aaaataggaa agaatgaatc gaaaaaaagg ggatgtctat ttttgacgga agagtctagt
      181 tctttagaga ttagattgac agctgctgat gatgtcagca tgcttttagg tgcacacgac
      241 aagcatataa aactgatcga ggaaacaacg gaaacaacca tccatacacg tggagaaatc
      301 attcagatca ttggtgaaca atcccagatt tccttggctg cctcagtcat tcggacatta
      361 caggaattga tcaaacgagg aatccacgta agtacaccag atgtcgtgac ggcattgaaa
      421 atggggcaaa aaggtacatt agattatttt gttgagatgt atgaggaaga gatcgtcaaa
      481 gaccgtaatg gaaaacctat acgggtaaaa aacagcggtc aaaagaaata cgttgaatcg
      541 gtagccaaac atgacatcgt atttggtgta gggccagccg gaacagggaa aacgtttctt
      601 gccgtagtat tagcaattgc tgccctaaaa aaaggcgaag tgcaaaaaat cattttgacg
      661 cggcccgctg tagaggcagg ggaaaaccta ggctttctac ccggggatct gaaagaaaaa
      721 gtggatcctt atctgcggcc tgtttatgat gctttatacc aaatttttgg attggatcat
      781 acgaaccgat tgatggaacg aggcgtaatc gaaatcgcac cattagctta catgcggggg
      841 cggacattgg atgatgcatt cgttatcttg gacgaagcac aaaacacgac agtcgctcag
      901 atgaaaatgt tcttgacccg tctcggttat caatcaaaaa tgattgtcaa tggggataca
      961 agtcagatag acttgcctcg agggacgaca agtggtcttg ttcatgcaga acgaacacta
     1021 aagcaaatca aaaaaatcga ttttgttaat tttgaagcaa gtgacgttgt tcggcaccct
     1081 gtagttgccg aaatcatcaa agcttatgaa aaggcggatc ttcatcaaga ataagctttg
     1141 cttaggagga taagcacatg ttgaataaaa cactgcagaa actacaagaa aaactcggaa
     1201 aggcagcatt gcctttcctt ctgttcttgt tttttatcat tgtctgcggc gttacattta
     1261 gtagtgtcaa acaaaaggcc aatgatttca gagaaggcca agtagcagaa gagagtatcc
     1321 gtgcgaataa aacgatcgag aacacggaag aaaccgaaca aagaagaaaa ctggcagcag
     1381 aagcagtgac tccagaatat acttatcaaa aagatctggc agatgaccag aacaatcgga
     1441 tcaagcaatt attcgaattg atcgataaga cgaatgatac catcaacaaa tcttataatg
     1501 acaaggtaga taaggcgaag gaagatgaaa atatcccgaa acctactaca gaagagcgga
     1561 ttgctacact gaagaaaaat tttgaaaacg tgaatgcaga aaacgttgcg ttttaccaaa
     1621 aactgccgaa caatttttat tcgacgatat ttgaaatgac tgaaacggaa attcacacgg
     1681 ttagggatga aagtttgaag ctcattgacg aacagatgag taaacaagtt cgtgaaagcg
     1741 aattggaagc ttttaaacaa gaagcagaag atcagattca gtatttgaat gtcacaacag
     1801 agcagcaaca aatgattcgc tacttagtgg atcaaggcat tgtagtcaat gatgtattga
     1861 atgagaaaaa gacagaagaa ttaaaacaat ctgcacgaga agctgtacaa ccagtgatga
     1921 tcttccaagg agaaatcatt gttcgcgaag gaaatcaaat tgatgcttca gcgatgaaaa
     1981 aattggaatt actaggattg acgaatcaaa cgacttcgat tttcccactc attgccatgg
     2041 ttttagctgt tttgcttcaa atcggcgtac tgatttataa ttcacttcaa ttccaaaatg
     2101 caggagaaag aacaaaatac gtgcttttct atgtgacagc tatgtcagtc agcatagtgc
     2161 tgatgaaatt tttccaatta ttccaaacgg aacaagctac ttatatcccg ctgttttatc
     2221 ccgctgcttt tgcaccactt gttttaagct ttttcttgaa ccgaagagca gggatcatgg
     2281 ctggtatttt ccaagcagta tctgcacttt tcatttttta tgattcgatt ggcacgaact
     2341 ttctaacgat tatcttgatg tcttatgttt tctctgggct catggcaacg attgtcagac
     2401 gcaaacggat ctcagaacaa ggattatctg ctgccatgtg ggtcattatt tttcctgtga
     2461 tgatggatgt cattttgatc gtctatcaag gaatgagctt tggtgatgca acgacttggt
     2521 taatgcttgt ctgcgggttc actggtgcac tcttctcatt cttgctgacg atcggtctac
     2581 acccatatat tgaattgatg gtcaatgatg acagtgtaat tgtattaaat gaactcagca
     2641 atccaaatca tcctttattg aaacagttgc tggaagaagc gccaggtact tatcatcata
     2701 gtatgatggt agctaatcta agtgctaacg ctgtagcaga aatcggcgga cgttctttgc
     2761 taactcgagt agcttgctat tatcatgata tcggcaagat caaacatgct agctttttcg
     2821 ttgagaactt gccttcagga gcggaaaacc cgcacaattt cttattgcca gaagacagta
     2881 aacatattat ctttggacat gtgacagatg gagccaaaat attggaagat tataatatgc
     2941 cgcaaatggt gatcgatatt tgcaaacagc atcatggaac cacgctaatg cgttatttct
     3001 acatcaaagc gaaagaacgc aatccggaag taacagaaga gcaattccgt tatcctggac
     3061 caaaaccgca aagtcgtgaa gcagggatcg tcaatattgc tgacagttgt gaggctgcag
     3121 tacgggcaat ggatcatccg acgagcgaaa aaatcgaaag ttttgtccat aatcttatcg
     3181 aagagcgtat ttccgatggt caattagacg atagtggact gaccttgaaa gaaatccgaa
     3241 aagtggaaaa atcattgatc agcggtttaa gtagcacctt ccattctcgg atcaaatatc
     3301 ctaaaatgaa aacagaagtt gagaagataa aggaagaaca agaagaatag aaagaagcga
     3361 tgtataatgg atattacctt tatggatgaa acaaaccaag tctcagaaga gaaaatgaaa
     3421 gaaatcgatg acttgctgca atttgcagca tcttatttaa agctgccaga ggaaacagaa
     3481 atgtctgtta ctttcatgga caatgcggca atccaagtaa tcaatcgtga ttaccgtgga
     3541 aaagatgcac cgacagacgt catcagtttt gctctggaag aagaaggcga agacgaaatc
     3601 ccagtgattt ttgaagatgg agagaatcct ttgcctcgtg tactaggaga catcatgatt
     3661 tctattgaac gtgctgctga acaagcagaa gcttacggcc attcatatga tcgggaactt
     3721 ggctttttag cggtacatgg attcttgcat atcaacgggt atgaccatat gacaccagaa
     3781 gatgagaaag aaatgtttgg tttacagaaa gagatcttgg atgcctatgg actcaaaaga
     3841 taaaaacgta gagaaaaaca aacatttcct gatgtctgtg gaatttgctg tccaaggagt
     3901 caaaaccgtt tttgaggaag aacgcaatat gaggaaacat gttgcgttcg gtatccttgc
     3961 tttatttatg ggcgtgattt tccagcttga tcggatagag tggttatggc tgcttttagc
     4021 tgtttttctc gtttggatag tagagatcat caatactgtt tttgaaaatg tagtggacat
     4081 gtttacggat tttcattttc atccgattgg gaaaaaaatc aaagatatgg cagctggagc
     4141 agttttagtg accgcttgtt ttgctgtcat cattgggttg attttatttg tccctaaaat
     4201 atatcatttg ttgtttaatt aaaaaggaga acgtatatgc cagaacataa atcagggttt
     4261 gtcgcaatcg tcggccgccc gaatgtagga aaatccacat tattgaaccg aatcgtgggt
     4321 caaaaaattg ccattatgag tgacaaggca cagacaacta gaaataaaat ccaaggagta
     4381 tatactacac cagaagctca gctgattttt atcgatacac caggaatcca taaaccaaag
     4441 catcgtctag gagattttat ggtagaagca gcttacagtg cgttgaaaga agtggatgca
     4501 gtcttattta tgatcagtgc tgatcaaaaa cgcggccggg gagacgattt tattattgaa
     4561 cgtctaaaaa acgtgcattc tccagtttat ctgattatca ataaaatcga taagatccat
     4621 ccagacgagt tgctgggaat catcgaagat tattcgacgc agatgccttt tgcacaagtg
     4681 attccgatct ctgctacaga aggaaacaat gtcgaacgct taatggatgt attagtcagt
     4741 gaaatgccag agggcccaca atattttcca gatgatcaag tgactgatca tcctgaatac
     4801 tttatcgttt ctgaattgat ccgtgagaaa gtcttgtttt taacacgaga cgaagtgcct
     4861 cattctgtcg cagtcgttgt tgattcgatg aagcgaaacg agaatgataa aatccatatc
     4921 caggccacga tcatcgtcga aagagatagc caaaaaggaa tcattatcgg caaaggcgga
     4981 aaaatgctga agcaagtagg aacgaaagca agaaaggata tcgaaaatct tttaggagac
     5041 aaagtatttc tagaactttg ggtcaaagtc caaaaagact ggcgagacaa aaaagtctat
     5101 ctgcaagact ttggctaccg aaaagacgaa tactgatatt ctgaacttct tataggttaa
     5161 gtatagaaat aaactggata aaaatccgca cgttgagaaa aacggcggaa gatcgaaaca
     5221 taaataatct ggatcggcag aatctgcttt tccagattta ttttttagga gggagaatat
     5281 gagccagctt gcagaatcga aaggcttgat tttatctagc cgtaattaca aagaaaaaga
     5341 taaattagtc aaaatcttta cggaatcttc tggcaaaatg atgttttata tcaaaggaat
     5401 ccatcggcaa aatcagccat tagctccggc tttgctcccc tttaccgaag cagtctatat
     5461 cggtaatttt cgaagtgaag gtttgtcgtt tttaagcagt gtcaaagacg tccatgcgtt
     5521 tcggaacatc caagaagata tctttttgag cggttatggt acctatttgc taaatctagt
     5581 agatgcagca atcgaagatc atcaatatga cccgcattta ttccaattta cgcaacaagc
     5641 tctgcagcgg atggatcaag gagatgatgc agaaatcatc acgaatatct ttgaagttca
     5701 aatactgcaa cgcttcggaa tcgcgccaat ttggacgcac tgtgtcgtat gcggggaaac
     5761 aaaaggaaaa tttgactatt cttccaaatc tggcggtgtg atatgcgaga aacactggcc
     5821 aatggacgaa caccgttatc atgcagatcc tcgcgctgtt tatttcgttc gaatgttttc
     5881 ggctatttct tatgataaga tcagtggaat ccacttgaaa gaagaaacga agcaggcaat
     5941 ccgtgacctc attgaccagt tgtatgatga atatgtcggt attcatctaa aaagcaaaaa
     6001 gttcattgat caaatgaagt cttgggcaca cgtattaaag acgccagaga aaaatatgcc
     6061 tgaaaaagag atacctgaaa aagaagaata aatgaaagat acaacaaaaa aatcaaaact
     6121 atttgacaaa tacagggact tccgtctatt attaaccgta gattaaatag atcgttcact
     6181 gagaggatct attatataaa caagaaaact gcaaaatgaa tacgtacatt gaccaagatc
     6241 agtaaaaaac acgtggttta gcgagttcgg gagagtggaa gccggaccca aacgattttt
     6301 gaagaggcac ttgcgagtac gataaatgaa gctgccgaat ctttattcgg aagtagggtg
     6361 gaaccgcgaa attattcgtc cctatgtctg taatcagaca tagggacttt ttgtcgtttt
     6421 ctttcctgta ttcttattga cagcaacgct taaaaattga aaggggcaaa acagatgagt
     6481 aaaaaattga ccgtccaaga tatgatttta acattacaaa aattttggtc atcaaacggc
     6541 tgcatgctga tgcaagctta tgacacagaa aaaggagcag gtacgatgag cccatataca
     6601 ttcttacggg caatcggacc ggaaccatgg aatgctgctt atgtagagcc atcccgccga
     6661 ccagctgacg ggcgttatgg agaaaaccca aaccgtttgt atcagcatca tcaatttcaa
     6721 gtcgtaatga aaccttcacc tgaaaatatc caagagcttt atttagaaag cttgcgtctt
     6781 ttaggaattg atccattaga acacgatatt cgttttgttg aagacaactg ggagaaccca
     6841 tcgatggggt gtgccggtgt gggttgggaa gtatggcttg atgggatgga gatcacacaa
     6901 ttcacgtact tccagcaagt cggaggacta ccttgtaaac cagttacttc tgagatcact
     6961 tatggactag aacgtcttgc ttcatatatt caagaagttg aaagtgtcta tgatctggaa
     7021 tgggctgatg gtgtgaaata tggcgagatc ttcaaacaac cagaatatga acattccaaa
     7081 tattcttttg aagtaagtga ccaagacttg ctactaagca actttgaccg ttttgaaaaa
     7141 gaagctaaac gctgtatcga tgaaaatctt gttcatccag cgtatgatta tattttgaaa
     7201 tgcagccata cattcaattt attagatgct cgcggtgcag tatccgtaac agaacgagca
     7261 ggttatcttt cccgtatccg caacatggct cgtgctgtag ccaaaatctt tgtggcagaa
     7321 cgcgaaaaat taggattccc tttattgaat aaaaacacag caaatccaga aaacatgaag
     7381 gaggctgaat aagatggcaa aagacttatt acttgaaatc gggctggaag aaatgcctgc
     7441 tcatgttgtc acaccaagca tgaaacaatt agaagaaaaa acagcaaaat ttttagatga
     7501 aaaccaattg acttatgata cgatcgaaac gttttctaca ccgcgccgct tagcaatcaa
     7561 agtaacaaac attccagaac gtcaagcaga cagtgaagaa gaagtcaaag gaccagccaa
     7621 aaaaattgct ttggacgcag atggaaactg gacgaaagca gctgaaggat ttgtccgcgg
     7681 acaaggtctg acgacagatg ctatcacttt tagagaatta aaaggcgtgg aatatgtcta
     7741 tgtaacgaaa tacactcacg gcaaagaagc gaaagaagta ttgacaggtt tagcagatgt
     7801 cattaaaagc ctcactttcc cagtaacgat gcattgggca gaatacgatt ttgaatatat
     7861 ccgccctatt cactggatcg tggcattgtt agatgacgaa gtaatcccgt ttgagatctt
     7921 agatgtcaaa acaggcagag cctctcgagg acatcgtttc ttaggcgaag atgtgacatt
     7981 tgcgcatgca aaagaatatg aagaaaaatt gaatgaacaa ttcgttattg cttcaccgca
     8041 aaaaagacaa gaaatgatcg ttacacaagc aaatgaatta gccgaaaaaa atcactgggt
     8101 tattgatcta gacgaagatt tactagaaga agtcaacaat ttagtcgaat atccaactgt
     8161 cttttctggc gacttcgaag aaaaatatct ttctgttcct gaagaagtac tagtcacttc
     8221 tatgaaagaa catcaacgtt atttcgaggt acggaacgat caaggtatgc tgcttcctca
     8281 ttttatttct gctcgtaatg gtgatcgggt aaaattggaa aatgtggttc gaggcaatca
     8341 aaaagtattg acggctcgtt tagaagatgc ggaattcttt tataatgaag acaagaaagc
     8401 ttcaatcgac caatacgttg aaaaactaaa aactgttact ttccatgaaa aaatcggtag
     8461 tatctatgag aaaatgcagc gggtacaagt cattgcccgt atcattggcg aacaagtagg
     8521 actatctgag gaagaattga tggatctgca acgagctgct gaaatctata aatttgatct
     8581 agtaaccaac atggtagggg aatttcctga actacaaggg atcatgggcg aaaaatatgc
     8641 tctgctgaaa ggcgagaaac ctgctgtagc aacagcgatt cgtgagcatt acatgcctat
     8701 atccagtgaa ggagagctac ctcagtctac agtaggttct gttttagcga ttgcagataa
     8761 attagatagc attttttctt tctttgcagt tgggatgatt ccaagcgggt caaacgatcc
     8821 ttatgcctta cgtcgccaag catacggtgt cgttcggatc atcgaatcga aaaaatgggc
     8881 ctttcctttg tctgttctgc aagaaacgat tagtgatgtt atcagtaaag atacggatcg
     8941 tttcggcatt ggtttatcag aagggcaaca acaggtgatc gactttatca aaggccgtct
     9001 gcgccaatta ttgacaacga agaatattcg tcatgatgtc atcgaagctg ttttgaatgc
     9061 agaacaaaaa gatctgacaa aagtatttgc agcagcacaa ttgttcaagc agcatcttgc
     9121 agatgaagac ttcaaaccat cgatggaagc tttgacacgt gtcgtgaact tagcgaaaaa
     9181 agcggagctt gaaaaacaat cagaagtaga ccctgaactg ttcgaaaatg aagcggagaa
     9241 agaactgcac aaagcagtag aggcaatgaa aaaaagcttt gctgaccaaa cgatcaacga
     9301 aaattatgat cagcttgtaa gccttcgccc actgatcgaa aattattttg atcagacgat
     9361 ggtcatggca gaagaaccag cagtacgtaa gaaccgatta acgcaattaa gtcaaattgc
     9421 tgccatggct ttgtcgctag caagtttaga tcaaatcatc acaaaataat ttctaatatg
     9481 taaataaacc agagaatagg cacaaaatag gctatttctc tggtttattt gttataaata
     9541 taaataacta tacccgtggt gctagttaat tttttcctag gtattataca agtaatttga
     9601 gtcggtcaat ttcaactgaa ttatctaaac taactgatct acttttagat aattcagttg
     9661 aaatttattg gtaaactgtc gcttttcttt ttaaatggca aaaattttta ataatatatg
     9721 acaatttaga aatcaactat aagactgatt aattcaaaat gatcttagaa tcattgtatg
     9781 aacactaaat actatattgt tttctattaa tcgttatata aacgattatg aattcaaagc
     9841 tactttttat gacgtcaatc agagagtgta aaa
//
LOCUS       DANKKG010000100         9842 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000100 DANKKG010000000
VERSION     DANKKG010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9842
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.100"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(129..491)
                     /locus_tag="PW004_002070"
     CDS             complement(129..491)
                     /locus_tag="PW004_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727031.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(565..945)
                     /locus_tag="PW004_002071"
     CDS             complement(565..945)
                     /locus_tag="PW004_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HDL2727032.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1009..2202
                     /locus_tag="PW004_002072"
     CDS             1009..2202
                     /locus_tag="PW004_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDL2727033.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            complement(2279..2863)
                     /locus_tag="PW004_002073"
     CDS             complement(2279..2863)
                     /locus_tag="PW004_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL2727034.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3064..4062
                     /locus_tag="PW004_002074"
     CDS             3064..4062
                     /locus_tag="PW004_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL2727035.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4174..4683
                     /locus_tag="PW004_002075"
     CDS             4174..4683
                     /locus_tag="PW004_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HDL2727036.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4713..5372)
                     /locus_tag="PW004_002076"
     CDS             complement(4713..5372)
                     /locus_tag="PW004_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HDL2727037.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5374..6138)
                     /locus_tag="PW004_002077"
     CDS             complement(5374..6138)
                     /locus_tag="PW004_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HDL2727038.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6146..6823)
                     /locus_tag="PW004_002078"
     CDS             complement(6146..6823)
                     /locus_tag="PW004_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HDL2727039.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(6860..8245)
                     /locus_tag="PW004_002079"
     CDS             complement(6860..8245)
                     /locus_tag="PW004_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL2727040.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8455..8539)
                     /gene="ffs"
                     /locus_tag="PW004_002080"
     ncRNA           complement(8455..8539)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PW004_002080"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8562..9077)
                     /gene="tadA"
                     /locus_tag="PW004_002081"
     CDS             complement(8562..9077)
                     /gene="tadA"
                     /locus_tag="PW004_002081"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HDL2727041.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9184..>9842
                     /locus_tag="PW004_002082"
     CDS             9184..>9842
                     /locus_tag="PW004_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HDL2727042.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     I"
BASE COUNT         2961 a         1972 c         1680 g         3229 t
ORIGIN      
        1 catattttga aaataaaatg cactaaaaaa ctatctttag cagaacattt cattgtattt
       61 tttggaccaa acaataaaaa aagaagtttg aaaaaaattc aaacttcttt gttcgcttaa
      121 tctgtatttt agaagaaagg cactccctct acttcgtaag ttccaagaac agagcgcatt
      181 tgacctggtt cttcccaaat atatccttcg cctaattctt ctctttgatt cttttgttca
      241 gtttcaaaga caaacaacgg aacattttcc tctttaatat gtgtatttgt caattccatc
      301 aaactaatct tgctaacatc gatatgaact atttctttta agaaattaag tatattcgct
      361 aaaccagtct ttccttcaga aagttccgct aacgcaaatt ccaacttgtc atcaattgaa
      421 tggaccaaaa attttttagt accatcctcc aaatgcaaca taattgtacc agcaactctt
      481 ttgcttgcca aaatttccac ctcgatcaac ttgttctatg gcttacataa aaattctagc
      541 acattttcag aaaaaataca ctttttattt cttattttct ttccgttcta gttcagataa
      601 tgcttcttct atccaaatag gtaactgctc cgcaatagtg gtaaatccga tcttacgatt
      661 tttattcgtg aaataccttt ttctgcgata tggaacagct ggatttgttt tttctaacgt
      721 tcttaaagat aaactgattt ttttggaata ttcatcaatg tcgatgatct gaactgttac
      781 tggctgtccg acttttaaca atgaatggat atttttcgta taaccggatt gtacttctga
      841 aacatgaata agtccttgcg tctcgttatc aagagaaacg aatgcgccat aaggctgtat
      901 ccctgtcact tttccttcaa ttatttgtcc aattttataa gtcatctact tccctctctt
      961 cgcatttatc attatactgt aaatagctca aaaaaagaag cgacgaaaat gactggaggt
     1021 cttaagatga tcgaatttga taaacagatt tcaagaaaag atacagaaag tgtaaaatgg
     1081 gacgcaattg ctgagacata tcaaatggat gacttacttc ctttgtgggt agcagatatg
     1141 gacttcttgt ctcccggtgg ggtagctaaa gcgtttagtg aatacatcaa acatggaatc
     1201 tttggttatg ccactctgtc tgataaacta tatcaggcta tcattcattg ggaacgccaa
     1261 caccatgctg tggagttgac gaaagaaaat atcgttttta caagtggtgt gttaaccagt
     1321 ttagctgcag cagtacaaac ttttacaaaa ccaggcgatt ctattttgat tcatgaccca
     1381 gtttatcctc cattttcctc tattatagaa acaaatcaac gtcacatcgt tcgaagctct
     1441 ttgctagaaa aaaataatca tttcatcatg gacttgtctg acatggagaa aaaaatcgaa
     1501 gaaaacaatg taaaagcaat gattttatgc aatcctcaca atcctggagg gcgtgtttgg
     1561 agcaaagaag aattgaaaca gttaagcgaa ttgtgtttaa aacatcaagt gattctgttt
     1621 agtgacgaga tccatcaaga tttaacttta ttcgatcata catttacttc catgctaacg
     1681 attgatccta atttagatga tctactagtt gcttttactt ccgcaaccaa gactttcaat
     1741 ctagctgcaa tcaaaaattc aatggtattt atcaaaaatc cagagatgaa agctgatttt
     1801 gaaaaacatt tagtgatgaa ccaacagcac gaaatcaaca cttttggact gattggaacc
     1861 caagcggctt atgagacggg tggagaatgg ttaagccaac tgattcctta tctagaaaaa
     1921 aatgttcaaa cagttaatca gttttttgaa cagtatcttc cgaaagtgag aataatgaac
     1981 cctgaaggaa cttacttaat gtggttagat tttaccgcct atatttcaga cgaccaagaa
     2041 ttggagaaca ctcttgttcg gaaaggaaaa gtcgtattaa atcctggcat cacgtttggt
     2101 ccttccggtc atgggcatat gcgcttgaat attgcttgtc cagaagaaac tttactggaa
     2161 ggacttaatc gaatcaagta tgctttagag acaatcgatt aaatcattta ttcaaaataa
     2221 aaggacgaga ccttactagg tttcgtcctt tttcataatt actccgtacc ataccaaact
     2281 attctgaaat ttcaattgtt tcaatcacta catcatgtac aggtcggtct tgtggaccac
     2341 gttgtacatt tgcgatttca tctactgtgt ccatcccttc tagtacatgt ccaaagactg
     2401 tatgtcggaa atctagccaa ggagttccgc cacctttata cgcttcaatg atttctgatg
     2461 ggaatccagc accttctagt tgtccaagca tattagctgg cacatttgtg ttatttacaa
     2521 taaagaattg gcttccgttt gtgtttggac ctgaatttgc catagataaa gctccgcgaa
     2581 ggttgaacag ttcacgactg aattcatctt caaaactttc gccataaata ctttcgccac
     2641 ccattcctgt tccagttgga tcgccgcctt ggatcatgaa atcaggaata acgcggtgaa
     2701 aaatcacgcc atcataataa ccttttttgg aaagttcaat gaaattttga accgtcttcg
     2761 gtgctagttc agggaataat tgaacagtaa tgtcaccacg gtttgttttg ataacagctt
     2821 ttggtccttt ttcacttttt aaatctagtt gtgggaatgc catattgttt cctcctaata
     2881 tttgcatact tcataataat aataacataa tacctaacct ctcgtaaaaa tactagattt
     2941 gttttctttt atttttcatc actagataca ttctataact taccgtttcg atttcgaatt
     3001 caaacatttt tgagaagcat tctcaattaa attctgatat agtgtgaaaa gggaggaatt
     3061 tttatggaaa tctatgacat tacaatcatc ggtgcaggtc ctgtagggat gtttgccgct
     3121 ttttacgcag gaatgcgcca agctaaaaca aaaatcattg acagtctacc tcaacttggt
     3181 ggtcaattag caactttgta tcccgaaaaa tatatttacg atattcctgg gtatccagca
     3241 ataaaagcaa gtgaattaat cgatcaatta gaaaaacaat taactacttt taaccataca
     3301 ttccatttaa aagaagaagt tctctcgctt actcgtgaag acgaagttat cgagattact
     3361 acaaataaag gcatccatta ttccaaagca gtcattttag ctttaggaaa tggttcattc
     3421 cagccaagaa aattgaattt agataatgct gaatcatttg aaaaccacgg attggattat
     3481 ttcgtgaatg atttgatgaa gtatgctgga aaaaaagtcg ctattgctgg aggtggtgat
     3541 tctgctatcg attggacgtt gatgttagag cctattgcaa gcgaagttta cttgattcat
     3601 cgtcgcccgg aatttcgtgc gcacgaacat agtgtatcac gcttgaaatc ttcatctgtc
     3661 aacttgctta ctccctatct catcgatgga ttatcaggca ataatgggga actgacagat
     3721 atccggttaa aaaaagtgaa aagtgacgag acgattgatt tgatgattga ttctctgatc
     3781 gtcaattatg gattctcttc taatttagaa catctttctt cttggggact tgatagtacc
     3841 agaaatgcca ttactgtaaa atcagatatg tctacttcta ttccaggcgt ttatgcagct
     3901 ggtgacatct gtacctatga aggaaaagtg aaactgattg ctactgggct cggtgaagca
     3961 cctactgcag tgaacaacgc tttacactat attaatccaa aggaacgaac tcagcctggt
     4021 cacagcacaa gcttatacga taaaaatctt cgtcctagtt gattttttat atctctttgc
     4081 atttcaagtt aaacccgaaa gcagtttctt gcattttttt catgttccgt taacatgaaa
     4141 tagagaaaga aaaaataaag ggggagcaat aatatggtcg tcaaaactga gactttagaa
     4201 gaaaaggcaa gagaactatt gaaggaacgt ggcgtaactg tagaagatat cgcacaactt
     4261 gtcatgtttt tacaaaaaga ctatatttct gatttgactt tggcggattg tactgaaagt
     4321 gtcaacagcg tgctaacaaa gcgtgaagta cataacgcca ttatcactgg aatccaatta
     4381 gatatattag cagaaaagaa aaaattgatg agtccgttgc aacatattat cgaagatgac
     4441 gaaggccttt acggtatcga tgagatccta gccttgtcaa tcgtgaatgt ttacggtacg
     4501 atcggcttta cgaattatgg ttatatcgac aaggtgaaac ctggtatttt aaaagaacta
     4561 aacagccacg atggcgttca tgtccatacc ttcttagatg atatcgtcgg agcgatcgcc
     4621 gctgctgctg ccagtcgatt agcacacaaa gaaccagaaa aaagaagtcg ccttactcaa
     4681 taattcaaca aaaaaagaaa gaaaacgtct atttagacga gttctttctt ttttgtttgt
     4741 ttcaagctat tttttcctaa gaaaagaaaa acagcaaaaa ataattctaa taatacaaaa
     4801 aataagataa agctatgcat gaaaaattct tctggaagta cattgatgat tggatcagta
     4861 acgggatcga ataaccagtc atcgttgttg aaaaacgttt catgaaaaag gataaaaaag
     4921 cgatcaaatc ctatcatcat gaaaaaaccg aaaacaactg gcagtaacat gccgatttga
     4981 aacggacgaa tcaaccgcca aagccgatca ttcttttgca aatatttgat aaacaaaata
     5041 ctcgggatca aagtgatgaa aaacacagca taatccacta aaaacaaaat tttcacttca
     5101 taaaagtgat gggctccgct agcagaaact gggaaatctg gcaagctgag gatggttttg
     5161 aagggattat tgagataatt cattaggtga tcaaaatttt tcagtaacgt ctcttgatct
     5221 aaagaagtat aatccaaaat ttgcaaatgt ccgatatcaa atacgtatag tggtcgaaag
     5281 ttgatcgtca caccaactgc cagactgatc agtgtcagaa ataggctgaa caatccagca
     5341 tattccatcc actgccatct cgaatttttc atattagaag ctccattcat ccagtgattg
     5401 tttcacatag gtagctggga taggcaaccc aggtacatct tcttcttttg taaaacctga
     5461 taaaacgagc aaagtatcta tattattgcg tatcccagct tggatatctg tttcatagtt
     5521 atctccaacc atgattactt cttctttttg cagccctaat acttcaacgg ctttatccat
     5581 aatgatggct tctggttttc cgatgtagat cggcggcgtt tgggttgctg tctctacaaa
     5641 agagatgact gaccctgctc caggaagaag gccgcgttca gtaggtatat tcttatctgg
     5701 gttcgttcca atgaaagtag cacctttttg aatagcaagt gttgctttaa ctactttttc
     5761 ataggtcaga taattatcta agcctaccac tacatagtca ggagcctctt cttcccatgt
     5821 aaatccggca gctaaaatca agtcaatcaa tcctgcttct ccgataacat agactttttt
     5881 cccttttcca tctgctttca taaaatcgat tgttgccaaa gtagctgtgt agactgtctc
     5941 tggagctaca tgaatgtcaa actcatctgc tagacgatta gctactgttt ctggtgactt
     6001 agtcgtgtta ttggtaacaa acaagaaagg taattttctt ttttgcaatt cttccacaaa
     6061 tcgtttaccg gccggtatgg gttgtgtccc acgataaatc gttccgtcta aatcaattaa
     6121 atatccttta tagtccattc atccactact cttccctttt tctgatcgta aatgctgttt
     6181 ttgttttttc tacttgttta ggttgttctt gtttttttac tttttcagag gtttttgctg
     6241 actctgtttt tcgattttta ataaccggtt ttttgcgatt agatgcgact ggtccttttt
     6301 tctcatcgat atacgcacga ttatggtttt ttttcttttt tcggcgctga gttgtctttt
     6361 cttttttacc gccgattctc tcgatgacaa aatacgcaca accaaaattg caatattcgt
     6421 aaagatagtc ttccaaggaa tcgattcgct gatctggatg cgcccgtcga ttatccgctt
     6481 ggaaaaatcc ttttaggcgt agttgttcat aaccccaatc tcctacgata taatcatatc
     6541 gggctagtac atcactgtaa cgttctccaa gcttttctgc attaaatcct tcacgataat
     6601 cggacacaag ccgataatgt ctttcaccta tcaaaaaatt actgtcatct aacagtgtca
     6661 ctagctcgcc tttgactttt tctgctttag gctcctcaac gatttcttcc aaagcagctg
     6721 tcagttcttc ggtgactgtt gtttcctgtt cagctgatga ttttttctta gcaatttctg
     6781 ttttatgctc aacgttctgc tgttcatttt gttttgcaga catgcgttca ccacctatct
     6841 tttttattat accttgtttt tatttgattg ggtagtgagc tttcaagtaa tctcctacca
     6901 cacttcggat aaattcaggg aacaaaaatt cattttttcc agcaatctca atcgttggaa
     6961 aaaatggaac aaacatcaaa tgatctacgg tagtaaaagt ataattttta ttcagatcga
     7021 ttggcttgtt cttccaaaag acttgatggt taatagaatc atatgtgatt ccattataga
     7081 ccatctgccc aaaaatctta ccgcgaaatc ccattccctt cataggatag ttacgtaaaa
     7141 agttacgatt tttttctatt tctaaaacta agcggatcag atcatttcca cccaatgtga
     7201 catttaacaa gtgcatcgga tgcggcaacg cagtgtgaag ctgatcctga ttgacccgcc
     7261 cttttggaat ttcagttaaa aacagaccac tgtttaatat tgctgcttcc gtctttcctc
     7321 gttctttcac cgcctcaaag gctacttgga taaacgaatg ctcattgaag atatctagac
     7381 tcagagaaaa tggcaaatct gctacttctt tttcttttaa taattcatgc ccacgttcta
     7441 gataactatt tatttcttgt tcatcttctg taaaagctgt cattgtttcg gtagggatgg
     7501 ctttggctga atgcttaagg atttttttct cttcatcgat ggttaaatgg acttcaccga
     7561 catactgccc gtactttcca gcggctgcta tttggacatc attgattacg tgaccttctc
     7621 tgaataaatg atgggtgtgc gatcctaaaa taacatcaat tgctggtatt tcttttgcaa
     7681 tttggaaatc atcctgaatc ccgagatgac tcatcaaaac taaaacatcc acttgtgaac
     7741 gtaaatcttc gactaattct ggcaaaatat cataaggatt ccgtatgtcc cagccattcg
     7801 gattataagt aagcggaaat ggagcagtaa atgcaattag tccgattttc gtctggtgtt
     7861 tcgttgtaat gattttatat ggtttcgtcc atgccggtgg ctggagcgta tttttatcga
     7921 ataagttatc caggatgata tcaaactcag catgatcgta taaatgattc aactcttctt
     7981 tggaatttcc tacgccttca ttgtttccta tcgtcgctgc atcataacca acttcattca
     8041 tcagcaagat attttcctgt ccattcgttg cttctgacaa tggatgccaa cgatccacaa
     8101 aatcacctag atctactgca atcgttgtcg ttttttcttt cttttcgttt tctcgtttac
     8161 gttgatccaa aaaccttctg atccttggcc aattttctaa atgagaatgc agatcattcg
     8221 tatgtaaaat cactattttt tccatgtacc ctctcgcctc tctttatctt tatcatacca
     8281 taggcagctt tttcttccat tgaaacaagc tctatttcag agtttttcct gccttgaagc
     8341 aaattgtata agtacaatat tcgataaatc agtctggcta aaaaatcagg aacattcatt
     8401 tttacagtac tataaaaata aaaaaaacag acccgaaagt ctgttgtcgt ttcattataa
     8461 tcagacacat ggcggaacct gcttagtgct gcttccttcc agacctgaca cgcttcacaa
     8521 gcccaccatt gtcctagcct ttcggcaaag aatagtatac cttatttttc attcgttgac
     8581 cagtcttttt ttaatttctt ttttcgctgg cggagctttc ggaaaaagtc agtcaaaatc
     8641 atcccacatt cgtcttctaa aatgccacct tctacatatg cttggtggtt aaaccgctcg
     8701 tcctccaaaa gattcatcaa ggtaccggct gttccgcctt ttggatcata cgcgccgaaa
     8761 taaacttctg ctacacgaga caaaatcatc gcgccgctgc acataggaca aggctctaag
     8821 gtaacaaaaa gtgcagcatc ttccaagcgc cagcttccca ctttttcaca ggcttccttg
     8881 attgcaagca tttctgcatg tgtagtagca tcttgcgtgg tttctctaag gttatatcct
     8941 cttccaatca cttttccatc caatacaacc actgctccaa tcggaacttc atgaagcatt
     9001 tctgctttct ttgcttctgc caatgcaagg cgcatccatt tttctttttc tactgcatca
     9061 agatttgtat ttaccaatta cccttccctg cctttgtttt ttcttgtttt actttagcat
     9121 atgataaaaa ggctgtaact aacttttttt tctatggtaa aataaataca agaggtgaac
     9181 aagatgaaaa tggataaatt acttgctctt tacccacaag cacagaaaaa aaactctcca
     9241 gcatcagaag cggatatcct ttcttttgca attgatgatg cttttatctg gatcaaacaa
     9301 gattcattat ctcaacaaga aaccagcctt ttgaaagcgt tattccctgt aatcaacgat
     9361 caaaaaaaac acccatggta tcactatctt tttaaagatg cttcttgtgt tcaagaaaaa
     9421 agctttcgtt tgatccagct tcacgtcgaa tcaaaaggag aattcttaaa aaaagaatgg
     9481 caagatacta ttttggaatt atttcgctat tctgctgatt tcttttttta tacagacaca
     9541 gatgcgctct tagtcgaaca aataaaccgt gattatcttg atgctactga aatcaacggt
     9601 atttttctat ctttagatgc tgattttgat acgacaacgt ctgtattcgt tggttctttc
     9661 cattcacctg gcaccgacct cgtccctttg tttgctgaag aaagaaaaat ttttttaaca
     9721 gagcaaaaca atctttatac tcacagccgt actttttcta tgcaagatgt ggctttacat
     9781 tactatacaa aagatactgt aaaagatagt aagttgatca aaagttacat agaactgatc
     9841 aa
//
LOCUS       DANKKG010000101         9674 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000101 DANKKG010000000
VERSION     DANKKG010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9674
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.101"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(<1..1620)
                     /locus_tag="PW004_002083"
     CDS             complement(<1..1620)
                     /locus_tag="PW004_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDL2727043.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSVLESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKK"
     gene            complement(1617..2069)
                     /locus_tag="PW004_002084"
     CDS             complement(1617..2069)
                     /locus_tag="PW004_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HDL2727044.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            complement(2181..2561)
                     /locus_tag="PW004_002085"
     CDS             complement(2181..2561)
                     /locus_tag="PW004_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDL2727045.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(2558..2944)
                     /locus_tag="PW004_002086"
     CDS             complement(2558..2944)
                     /locus_tag="PW004_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727046.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(2946..3224)
                     /locus_tag="PW004_002087"
     CDS             complement(2946..3224)
                     /locus_tag="PW004_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727047.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            3272..4000
                     /locus_tag="PW004_002088"
     CDS             3272..4000
                     /locus_tag="PW004_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HDL2727048.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            complement(4146..4622)
                     /locus_tag="PW004_002089"
     CDS             complement(4146..4622)
                     /locus_tag="PW004_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HDL2727049.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(4582..4929)
                     /locus_tag="PW004_002090"
     CDS             complement(4582..4929)
                     /locus_tag="PW004_002090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727050.1"
                     /translation="MNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVLKIE
                     QNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIRGGV
                     CEISLVKRLPRTR"
     gene            complement(4916..5101)
                     /locus_tag="PW004_002091"
     CDS             complement(4916..5101)
                     /locus_tag="PW004_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727051.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            complement(5102..5374)
                     /locus_tag="PW004_002092"
     CDS             complement(5102..5374)
                     /locus_tag="PW004_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HDL2727052.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            complement(5371..5574)
                     /locus_tag="PW004_002093"
     CDS             complement(5371..5574)
                     /locus_tag="PW004_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727053.1"
                     /translation="MVKVREQFKIVSDEGNVYKIKIVNINEYREADHKYLVDLYMNDK
                     YLGEGFCGDSLFDDERILKEETE"
     gene            5699..5911
                     /locus_tag="PW004_002094"
     CDS             5699..5911
                     /locus_tag="PW004_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727054.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKYYSDYWTQTRENELNLEKLIIKKK"
     gene            complement(5944..6138)
                     /locus_tag="PW004_002095"
     CDS             complement(5944..6138)
                     /locus_tag="PW004_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727055.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(6151..6333)
                     /locus_tag="PW004_002096"
     CDS             complement(6151..6333)
                     /locus_tag="PW004_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727056.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKI"
     gene            complement(6330..6470)
                     /locus_tag="PW004_002097"
     CDS             complement(6330..6470)
                     /locus_tag="PW004_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727057.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            complement(6960..7325)
                     /locus_tag="PW004_002098"
     CDS             complement(6960..7325)
                     /locus_tag="PW004_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727058.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            complement(7365..7667)
                     /locus_tag="PW004_002099"
     CDS             complement(7365..7667)
                     /locus_tag="PW004_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HDL2727059.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            complement(7667..7966)
                     /locus_tag="PW004_002100"
     CDS             complement(7667..7966)
                     /locus_tag="PW004_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727060.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            complement(7959..8123)
                     /locus_tag="PW004_002101"
     CDS             complement(7959..8123)
                     /locus_tag="PW004_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727061.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            complement(8129..8398)
                     /locus_tag="PW004_002102"
     CDS             complement(8129..8398)
                     /locus_tag="PW004_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727062.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            complement(8410..9159)
                     /locus_tag="PW004_002103"
     CDS             complement(8410..9159)
                     /locus_tag="PW004_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2727063.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            complement(9156..>9674)
                     /locus_tag="PW004_002104"
     CDS             complement(9156..>9674)
                     /locus_tag="PW004_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727064.1"
                     /translation="VQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGGEK"
BASE COUNT         2698 a         1978 c         1483 g         3515 t
ORIGIN      
        1 ctttttcttt gattgacgtt tacttggcgc tttttgtcca ttgatgttag taattgctac
       61 aacgttcaaa gtgcaataaa ggaataatgg attatcaaat tgaattcgtt tctcataaaa
      121 caaccgttcg acatcgtcaa aaggagcatt caatacttta ggatattgag caacttcaac
      181 gcattccaat cctaagtttt ctaatttctc aacaagtttg tcactcatcg ctggatcata
      241 attcacttgc tgaatatcat ataaattcat gcagtcctct atgtactgca aaatctgatc
      301 ttgatcaatc atttttccgt cgcaaaattc aacgaaacct tgttcagcca aatcactgta
      361 aggaacatta tcttcttttt ctcgaaactc taaatcttca ttcggaataa aataaagttg
      421 ctttacttta agaaccgctt taccatcttc atcccatgtt gggaagttta aagatacaca
      481 ggtcaaatct cgtgtacgtg ataaatctaa accgatataa catggttcgc cacttaaatc
      541 accaagttct tgtgtggtaa ctaaacatgg ctctacttga tcctgttcaa agaaattatc
      601 cgcgccgttc acaaatacat ccaagtgttt cgttagaaat tcagctttgg aatgagctga
      661 ccgttgcgct gttttaaatg ccgattctaa aacagataaa tcgactgata ttccccagtt
      721 aggattgcac atttcccaga cttttctatc cgtccaatca taatttttat ttggctcata
      781 gattagaaca aagtttgaat cattatcatc acgtttcaac acttcttttg cttcgcgata
      841 tacacgcatt ccaacagacg acgaaccttt cccagcagtt gagatattaa acatcaatgg
      901 ctgtggtaaa gatatctgag cagacttaaa attgtcatac tgttccattt tctcctgttt
      961 atgcaattca tcgttcaata caaaatatgg attggatccc tcgatattat caatattctt
     1021 ggtttgaaca ataaatttat ttgagtaagc catatcttca tgaatatagt cataagtaat
     1081 actagaaaca gttccctttg gccccctaaa tattttagtt ccttcaagta ggattggatt
     1141 gtttaagatt gtaactgcaa aaggtttggc agcatattgc gcttgagcaa aatcagatgc
     1201 gcaagcatag caatcaactg ataacgcccc ttctccgtac attgcatatc ctaatgaacc
     1261 gacagcaatc aatgttttac cattcttttt tggaatctgg acataagcct cacgagtaac
     1321 gcgtacaatt tggccttttt cattttcttt aacccaacca taaatccaag aataaataaa
     1381 tttttcccag ggttctaaaa gaaacggttt tcccaccatt tctccttttg tgtgaacaat
     1441 aaatgattct acccaatcca tcatttcatt cgcgcgatct acatcaaacc aaatatcttt
     1501 tctttttttc cactgatacc agcgatctat tgctaaacga acggtcttag gatacttctt
     1561 aggtttcttt cttacttctt ttgcaaataa atcagcataa tttacaccag gttcaatcat
     1621 ttttcagcac ctgccttctt acgccattta tttctgtgcg ctgctagttc atctactggc
     1681 tttacttctg ggcgtgtaat ttcttcatct tttcttgcgg tcgatcctcc agcaattagt
     1741 ctaccagttt ttgctttgtt tgttaatcct aataaatcta gagctttcat cttcttatct
     1801 gcccaagttt ctacttgttg tgccaatgga tgttttgatt gattagtagc tcctgattta
     1861 ttcgtgaatt tttgcgtctc cggaaatcct ttttccttcc acaataaata tttgtgttgg
     1921 taaatttcaa aaatatccaa gtatgattcg attaatggat caagagtgat ggtgtacaaa
     1981 tcagacaaat tcattatttc taagatacga gctttttcag cacttacttt ttcatcaata
     2041 atcgctttac gttgcgcttt agtcgtcatt tttgtataca cccccctttg ttttttgaaa
     2101 aatttgacct aacgatgcgc gtgactcccc gctaccctat ctccccacgc gaaaaaattt
     2161 tgaaaatgaa taggggggct tcaattgaaa tacgaaggaa aaactttttt gtcttccgtt
     2221 tcgttttcaa caattggatg acattttgaa cataatagca tgagattgtt tggatcaagc
     2281 ttaagcagtt cgttgtcttt gattggtaca atgtgatgga cgtgtgctct ctttccaaat
     2341 atgaactgac cacaccgctg acaatgacca ccttctcttt cataaataaa ttgacgcata
     2401 tctttccatg cttgcgttcg atagaatggt ttgttctcat gatgataaac agactttgct
     2461 tgctgcttct tcttgcgtga tctgcttgat ctcttgtgtt cagtacagta gatacccttt
     2521 gctattttgt tcgtgcatcc gtcaaactga cagtatttca ttctacttca cgaataagat
     2581 ttatgatatc gccttttgca cggatagcac caggaatatc aataccatgt ttcttagcat
     2641 atgcacgcaa ttcttttgca gtcatattgt ccagttcatc cgtatcttca gtggactgat
     2701 cattagtagc ttcgtctcca tcaaaattag cagcagtgtt tccatcgcta tcaagaattt
     2761 cactgctatg agctatgagt tcgccattga cagcaacaaa tgatttgcca tcactcaaaa
     2821 ttcctttttc ttcttttact tcaaaatttg gttcttgatc tttcggaaca actactgtcc
     2881 ttttcttttc tgaatcccaa tactctgttc cactaatgga tgttctaatt ttgatcattg
     2941 ccattttaat tctctccttt gtaatttgta cggattactt ttgcgcccat tcgctcatac
     3001 cattcaatcg tttcctgcag attgggtaga gtgtgtgaca ataaagaaat aactaacgta
     3061 ttcattccag ttctgtaatc atcattctcc acttgcacat gtgcatgatt accattccaa
     3121 ttttctctgt acggactatt ctctgcttca attgctttct tataaacttt agccaaactt
     3181 gattctactt caatactaag taccgcttca tgaaaatcat tcatttgttt actctcctat
     3241 cagatatttt gtggtatgat ttataaaaaa gatgcgaggt gttttaatgc ttcaagaagt
     3301 taatattaag tatcgtgatc gtcaaaatac agagacaatt tatttgccaa atagttgtcc
     3361 gcattgcgga aatataatga ctccgtatgt gtattttggg gtttcatcat atagtgcgtt
     3421 cgataatgat agaatctttg gcgtattagt acagtgtata aatgatgagt gcaacaaatt
     3481 ttatagttta caatttataa atacgtttaa tgaagatgaa ggatattctg aaaatatttt
     3541 aattaaagat tattaccgtc cgccgattaa aatagattta ccagaaaata ttgaaaaagt
     3601 ttctcctaca tttgttgata tttatactca agccacacaa gctaaatctg agcaattgga
     3661 acaaattgct ggagttggtt atagaaaagc tctagaattt ctaattaaag attatgcaat
     3721 atctaaaaat tcagaggatg aagataaaat aaaaaaaatg ttattaggta aaacaattga
     3781 aaattatttg aatgattttc ctaaactcca aagattagcc aaagctgcta cttggattgg
     3841 taatgatgaa acgcactatg ttcgtagaca tactaataaa gatgttcatg atatggatgc
     3901 atttattaaa tcagcagcgc aatttatcgc ggctgattat gatgctgatt tagctgatca
     3961 atttataaat ggaaataact ttatgccaac tactaattaa aagaataaaa gagccacaga
     4021 taccaattaa taaaacggtt gggatatctt ggctctttca tatttttttg acactaatag
     4081 aatatcatgc tgctaacaat gtttacatag ctacaatgac cgaacattta gtgaacagtg
     4141 taattttaga ataaagagag ttgttctttg tacttctcgc tattgtaaaa atcaatttcg
     4201 ttttcttttt tcaatcggtc agcttgtctg tgttcatact catctaaaaa gttcaatgtc
     4261 tttctaattt ctgcatgtcg ctgtctgaca tacgatgagc tatacccaac agattcagca
     4321 atatcttcta agctcatttg atcaacatat ttcatcttaa caatttgatt atcgatacca
     4381 gaaaagctat ctatcagcaa taacatttct tctttttgct cgatcaacaa ttccaattca
     4441 ttttctatct tttggatatt ctcttctaaa gaagaagttc tcgaattctt ttcgatgcgg
     4501 acgtttgcta aatcaccaca gacccatcga ttcaattcaa gcttactttt attaagattc
     4561 cactttaagt aaagaatctg ctcatctagt tcttggtaat cttttaacca atgaaatctc
     4621 acaaacgcca ccccttatgg tagaatagta ttgcgctgct atctcgaaag gggtagcttt
     4681 tttatttttt cttagccgtt ccattctgga ttgccttttg ttccaaacgt cgttttttct
     4741 tcttaatctt tgattttgtt tttcccatac aattctcctt tccgaaaaca cattttgttc
     4801 gatttttaat actaaataaa agttggccag aatttttcgt tcgtctttac tcatcttttt
     4861 ttgaataaga ttactagcat aaaaatagtt agtaccacga aaatgaacag gtaaatcaat
     4921 tacgttcacc ttcttcatgc cagttatcag ccactagaat ctttgcgtta gtgccgccac
     4981 cgttttcttt tttgtatttt tcaaatgctt tataagcagc tgtttttgat ttatagattt
     5041 ttccggaacc gactgggaac atccatgtgc cctctgtgat agctaagatg tatactttca
     5101 tttaattcac ccctttaatc cccaacacta catatccgga cttctgcatg taatcagtaa
     5161 tataagtgat ctctgctttg tatgagtcac cagtgtaact aaatgttggt ctcgttagtc
     5221 caggtaattc atgtttaatg tattttttat attcacgtag tattaactga tcaccaactt
     5281 tgaagtcacg atcattcttg cggatctcga attgtttgcg tccgctcgtc actgcttcaa
     5341 aatattctgg tagtattttt agttcgtgga tcattctgtt tcctccttca aaattcgctc
     5401 atcatcaaaa agtgaatctc cacaaaatcc ttctcccaaa tatttatcgt tcatgtataa
     5461 gtcaaccaaa tatttgtgat ctgcttctcg atattcatta atattaacga tctttatttt
     5521 gtaaacatta ccttcgtcgc tcactatctt gaattgttct ctgactttca ccaccgttcc
     5581 tcctcttttt gaaaaaattt tagtgttttt gtaagacccc ctaaatcagc ttttttattt
     5641 gatataatac ttgtatcgat taatcaaatc gaaattaaat ttaaaaaggt gatttatcat
     5701 gaatatcaac aatttagtaa aactcgttga acaacgtttt aaagaatcag gattagaaca
     5761 attaaaaaaa gataattctc cagaattatt ttctaatttt accgaagacg acattcctac
     5821 tcttaaacaa ttaaaatatt attctgatta ttggacgcaa actcgtgaga atgaattaaa
     5881 tttagaaaaa ctcattatta aaaagaaata gcaactccag cccacacggc tggtttttta
     5941 gtttcaactt ccctccttac gggtcaaaat catcggttcc ggcataatct gatcatcctt
     6001 tagcaacaaa tcaggatatt ttttccttag cttcttattg ttcgtgtaag ttttcaatag
     6061 ccattggttg gcttcgcttt tgctttctcc agaaaaataa attttccctg tttggatgcc
     6121 tgcaagttga atcatcattt tcacctcaaa tcaaattttt tctgctagat ggagcaaaag
     6181 caatccgaca tcaagaatct tttgacccat tatttcagct actttgctcg gcttaactcc
     6241 ttgagaaaag agcaagcgtg cttgctctat ctcttcgtga gtccaaataa atttggcttc
     6301 ctcaaggata attactggat cagttgctct cattgttcct ctggaaaaat gatgtcattc
     6361 ttatgcttgg accaggtatc tgcatatgga acaaagtatt gtctagctaa ttcgatttga
     6421 tcaattaaag cagtttctga taattcatgg tctgcagcaa tttcattcat actatcgcct
     6481 tcatctaaac agataagacc gttgacgtta tcgattttta catttgtgac tgcttgactt
     6541 tctccacgct cataatattc tacagttgat actgatacat cagcttcgga gtttaattgc
     6601 agaatctgtt actgccggga taatttaata ttcttttcat aattcacctt ttttcctact
     6661 gacatcgaat atttttcatg ttatgattag ccgtgaaagg agagctatat tatgtgttta
     6721 cacatgatgt ccttagctca ttcggttctt tggaagcttt tttggaaata actctcaggt
     6781 tcttgacgca aatctcattt attctttgaa acaaaaaact tccattttcc gagagctttg
     6841 gatcacttca aaagagattt gcatttatat ttctgtgacc ttgagtcgcg taaagaacct
     6901 tacagcaatg gcggcaattc cactttcagg aaaccgcctt ttttcattga tattggtggt
     6961 tagcggacta cccattttct ttcggcagtg ccaaaccaca aatgatctgt gttgatgttt
     7021 aggtagttgt ataccagata agctaaatcc aaagtgcctg catcttcaag acgacaatat
     7081 tccaaaaatt ctcccatagc tggacataaa aatcctggag ctatcatatc ccaacagtta
     7141 atagtgcaaa gaaattcgtt tagcttctga acgttttctt tggtttcacg aattacaaca
     7201 tactccaatt cttctccgct ggcagagcat tccaaaactt caaattgatt aagaaatggt
     7261 agaacatatg cttcaaacat caacattcca tctttctgtt tttgcacttg ttttgcatca
     7321 atcatgtcta tcctcctatt cagttatttc ttccattaat ggaattattt caggtcactg
     7381 gacttaacga aaacaccatt gaccatcttt ccagtccgac ctttgatttc gtcataagca
     7441 aaatccaagc actcgtataa atccatatca ttttgcattg ccaagatgat caaggttact
     7501 actacatcgc caattccgtc tcttaaacca tgttcatctt ttctagctag agaagcggca
     7561 acttccccga tttcttcgac cgtttttaac atttgcttgc tgaaatccgc ttgatccaat
     7621 cccttatctt ttgcccactg ctctactttc gcgattagtt cgtccattat ttctcctcca
     7681 catacctaaa ctgtcttcct tttgaatcaa tccataagct cctagctcta tcccaaataa
     7741 tatttttgct cagaccagta atttcagata actgtttagc ggtacccgtt actagaattc
     7801 gatcaccatg ccagattgca atctttctcg gcgttctccg tttgggcttt tcagtccaca
     7861 ttgatttacc gagcttttgg acttctgcaa ctattttttt atcttcctgc caatcttctg
     7921 aataagtcaa ttcgataatt cgctgcattg ccgctttctt atccatcccg acattctcct
     7981 ttcagtaatt tgagtacttg atcaagtgcg ctctcacgtc cgccatgaaa tgtgttgagc
     8041 cacttgtctt cgtacgacac actttgtctt aaagcttctt gatgcattaa ttcgatctgt
     8101 gctgtaaatg tctttaggtc catcttgttt acacctgctc aagttcatta agatgttttt
     8161 gcaagccttt aacacaatca atgaatagta atttggtata tgctaaattt cttaattgcg
     8221 ttccatcgat atagagtgcg aaatagtatc tgagtttact ccaactcgaa cgatcattct
     8281 taattccttc aattccagct tcttcgagtt gatcatatac gtctctcagg acgtctaatt
     8341 cctcgccctt tttataattc gctatttcat taattagttc tagataatcg atcttcattt
     8401 ttccacctct tagaatggtg cttttgattg tctattagct cgttctagcg cttttttctt
     8461 gagataggct tcttggtcga ttgcccattc aggaagcttc tctcgtcttc ctgtgcgctt
     8521 gtatccactg cttgcgttct taggttcact tttttctttc cttgcccaac ttcgaatagt
     8581 tgccaaatag tttttataag tcttaccaga tgattcacaa tactctgaca gtcgttcgat
     8641 tcgctcttgg taatcattag ggaattctgt tttgagtttc tccatctgct catctgacaa
     8701 aagaacattt ttatactctc cgtatttatg acggacgggc ttagccttcg attttttcga
     8761 aggcggtaac tctcttatat attcttttgt attattaaat gtattattaa tagatgtatt
     8821 attatctttg actttttcgt caataggggt attgcgtttt tcgtcaatag ggtattgatt
     8881 aattcgtagg tacctattga ttatttgatt ggtaccctct ttgtaaatga tttcccgatt
     8941 caagtatcca aacttaatca aatcacttac ccatcgcgat atggtctctt tattcacacc
     9001 atataaatct gcaaagtact cattgcctgc ccaacaaaag cctctttcat tacacaaggc
     9061 cgttatctct ccgtataaca acttagtatt tggtttaagt cttttgtcgt accttacgtt
     9121 ggctggtata atcgcataat aacttcgatg ttctgtcatt tttcccctcc aatatttaac
     9181 tttttgattg ttttctggtt taatttgatc cctttgattt gatatttatt tttgaaatta
     9241 atcacaccta ttttgtgctt ctctgtgtga tggattctgc agagtgctgc aaatgtgtac
     9301 tctgcatgat caacttcttt gcgctttcgt cttcctagcg ctttgtcaaa gtgatcgatg
     9361 tcagctcctg ttttgccaca gatgcagcag actctttttg tgatgcattt gtagaagtaa
     9421 tattcttgat tcgcaggtaa aatctcatag ccttctttga aaggaatatg atgttcaaag
     9481 atgaaatcta agatgatatt tgctaagaca ttggcatcac tcacagttgt attcgattcg
     9541 tctttcaggc ttattttgcg ccctgtgaca ccttcaaaac gaaagtagaa gaattccttc
     9601 cagaagtccg ttggcatgcc tgtatcgata aaaatatcgc ctatcagcgc atagatgaag
     9661 tttcgttgct gtac
//
LOCUS       DANKKG010000102         9608 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000102 DANKKG010000000
VERSION     DANKKG010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9608
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.102"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            960..1928
                     /locus_tag="PW004_002105"
     CDS             960..1928
                     /locus_tag="PW004_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDL2727065.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            1945..2469
                     /locus_tag="PW004_002106"
     CDS             1945..2469
                     /locus_tag="PW004_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HDL2727066.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINE
                     TGQKAIKSMFFNENENSNQQLVGEKTKTENHKNANQVPELEVLSLLEQQLNQKDMQLQ
                     EKDEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIE
                     KQSKAEKKWWQFWQ"
     gene            2845..3126
                     /locus_tag="PW004_002107"
     CDS             2845..3126
                     /locus_tag="PW004_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727067.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            3128..3544
                     /locus_tag="PW004_002108"
     CDS             3128..3544
                     /locus_tag="PW004_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727068.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
     gene            4174..5328
                     /locus_tag="PW004_002109"
     CDS             4174..5328
                     /locus_tag="PW004_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HDL2727069.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            5322..5744
                     /locus_tag="PW004_002110"
     CDS             5322..5744
                     /locus_tag="PW004_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727070.1"
                     /translation="MVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            6055..6435
                     /locus_tag="PW004_002111"
     CDS             6055..6435
                     /locus_tag="PW004_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HDL2727071.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            6417..7331
                     /locus_tag="PW004_002112"
     CDS             6417..7331
                     /locus_tag="PW004_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HDL2727072.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            7291..7569
                     /locus_tag="PW004_002113"
     CDS             7291..7569
                     /locus_tag="PW004_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727073.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            7576..7779
                     /locus_tag="PW004_002114"
     CDS             7576..7779
                     /locus_tag="PW004_002114"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727074.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLTELGRSRPSSIGG"
     gene            7900..8304
                     /gene="tnpA"
                     /locus_tag="PW004_002115"
     CDS             7900..8304
                     /gene="tnpA"
                     /locus_tag="PW004_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HDL2727075.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
     gene            8321..9469
                     /locus_tag="PW004_002116"
     CDS             8321..9469
                     /locus_tag="PW004_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2727076.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
BASE COUNT         3602 a         1682 c         1642 g         2682 t
ORIGIN      
        1 cactttagtg agttttgtag aaagtacctt taaaggtact ttcacattac gaacgatacg
       61 tccgtaggac gtactgaaag ggtgggattt atgagcgaac taatgcaaaa tttggctagc
      121 aaaaaaagtt aaaaaatata tgctaataat cgagcgaaat gaacactttt tcaagtgagc
      181 gaatccgcca tgacaaagct gaagcagttt atttcagctt tgtcatggcg tgtgcctaga
      241 cttttaaaaa agtcggcaca cgagcaaata tctttatcaa gctctaattt gcgttttaag
      301 acgtttgaaa cgtctaagta tataaatata taagagtagg tttaagagtg cttaaatcga
      361 attttagagg gcattcagac aactttaggg ttgttagggc ttgccctgac cgcctgtaag
      421 gcgctttaag aacaagataa gggatttata ctaagactgt cttaaaacgt ctcaaacgaa
      481 ggctcatagc ttctcaaaga gttccttatg aacattttta gcaaaggagc cggaagcgac
      541 tgcgcagtat aatgtgagag cagagcgatc acaccttttt aagcgactgt agggagtaag
      601 attcccagtt gagaaaaatg cgcgaatctg tttggagaaa aaagaagtgg ttttttaggc
      661 gggaaatttt gtccccctct tttataatct ctttttaaac ctctttttaa acctctttac
      721 cgtcggctat atccttactc tcccaaggga atgacgcttt tatctacatc gttttgtctg
      781 tttatctaca tcgttttgtc tgtttatcta catcgttttg tctgtttatc tacatcgttt
      841 tgtctgtttg tacattgaat tagtacatcg acgatgttaa tataagatgt agataaacag
      901 gcattttgta tttcataacg tgtatctctt gtgcgagtaa gaaaggagct attttaaata
      961 tgtctaatga aatcgtaaag tatcatcatg aattaaatac aattccgttg cgaaaattta
     1021 caccagtcga aatgaatttg ttcttttcta tcgtatcaag aatgcgtgat gtaggtgata
     1081 aaaaagtaca atttacattc gagcaattaa aagatttaag caattataaa gcaactgcaa
     1141 atgttcgatt catagatgat ttagaaacaa cgtatgacaa attaatggat ttacgttttg
     1201 gtagaagaag tgctgacggt ttgcaacgtg aacgttttgt tttatttaat caatttaaaa
     1261 tcgacggaaa atctgatatt ccttttgctg aaatacaagt tcatgaaaaa gccttaccct
     1321 tacttaataa tcttgaagaa tgggtgcgct attctctcca acaattcaat gaattagaaa
     1381 gcagttattc aaaaacaatg tttcgattac taaaacaata cagaaccaaa ggtttcgctt
     1441 atttttcaaa agaagacttt catgaattac ttgatattcc taaaagttac aaccaaggta
     1501 cgattgatca acgtgtgttc aatccaatac gacaagaact aaccgcaatc tttaaaggtt
     1561 taacaatcaa aaagaaatac ggaaaaggac ggggaaagcc tgtcattggc tatcaattta
     1621 catttaaacc tgagataaaa aaatcagacg actttaacaa acatgcccca acaaaagaag
     1681 aacaaaacaa agcattagaa ctagctaacc aacaagttca atctgaaaaa gcacctactt
     1741 cattacaaga aaaaatcaaa gaacaaaagc gaaaattgga acttttacaa cagttagaaa
     1801 agttagaaag agaacaaaat acccaaaata aagaaaataa tgcacaaaaa tcagagataa
     1861 attccgatat tccttcagaa ctaaaaaaag acttattaga caatcttcaa aacttattca
     1921 aagattgagg ggtttttaga ttttatgaca gaaaaaacaa tcaaagaact tgctgaagaa
     1981 ttaggagtta gtaaaacagc aattaacaaa aaagtttctg acgaaaatcg aaaactttgg
     2041 ttttcgaaaa tcggaaacaa gtttgtgatt aatgaaaccg gacaaaaggc tataaaatca
     2101 atgtttttta acgaaaacga aaactcaaat caacaactag ttggcgaaaa aacgaaaacc
     2161 gaaaaccaca aaaacgccaa ccaagttccc gaattagaag tcctttctct cctagaacaa
     2221 cagttaaatc aaaaagatat gcaactacaa gaaaaagacg agcaaataaa acaaatgcaa
     2281 aaattactcg accaccaaca acaactcacc ctgcaagcca accaacaaat cgagaaactg
     2341 caggaacaac tacagttgac ttatacggaa gaatcttcgg aaaataaatc aacgactctt
     2401 tctgaagaaa cagaatcgat agaaaaacaa tcaaaggcgg agaaaaaatg gtggcaattc
     2461 tggcaataaa accagcaatt taaattaata aaaattgctg gttttattct tctttttgct
     2521 tttgcgagca gagcaacatg gacccatgtt gcgatatttt ggtggcgccc caatccccgt
     2581 tttttcgttg aatcttactc ggactgcact cgttttacag ctgatttttc tgaattttga
     2641 aagagcataa ggaaacgtgg tatcactttc ttacgaaagg tgctagatag gctgtgccta
     2701 tcacgtttct cagaaaaaaa tttgctatca cttttttgcc attgtggcta ggctatgtat
     2761 agtgttttgg cgaaaaatag ctatacaaat actatatttg tatggtatac ataaattata
     2821 cgctgatacg aaaggaaaat ttccatggac attttagttc gaaatatcaa tcccaaaatt
     2881 attgcaagaa tagataaacg ttgtcaggaa ctcacaacca aaacgggcaa aaaatggagt
     2941 cgaaataaat atctaaaagt tctaatcgaa agtgattttg accgtcaact tatgaattac
     3001 aaaaaagatc aattcgattt attactcgaa aaatttattg ccattcaaac agaaaatacg
     3061 aacatactga atgactatgt tcacacaaac aaccaactca ttgaaacact tatcggagga
     3121 atataacatg agaaaaactt ctgttcaaaa acacgtaacc ctttatccca gtcacgttga
     3181 cgttttagat tccatcatcg aaaaaaatcc agacgtcaac aattattcag aagccattcg
     3241 ttttttatgt atgagttatg aacctgatac caatacaacc acaaccagta accaagcaaa
     3301 actcaatgcc atgggaaaag aaatttctat catgacagaa atcatggcag aatttgctga
     3361 tttatatcta aaagatactg gtgttcttat cggtacaaat tctaaagtgt atcaagaagt
     3421 gaaagaaaaa attgaaaatc aactcaatcc ttcccgacga aatcaaccca aaaatcgtca
     3481 aaaagaaacc aatcaaacct ttacttctgg aaattttgac ccaacaaact ttctaaaaag
     3541 ataaaaaaag aaaccgatcc ctaaaaaaat tcctcgattt ctttttaggg atcagtcttc
     3601 cagcaagatt tgcaagttgg taccaacttg cgaaaaaaaa gaaaaatccg cttatgcaac
     3661 ttcttctttt tgtattttgg ggcgctaccc caatccccaa gtatcggtta tttaatagcg
     3721 cggaaatcca aagaatagga cgtaccctga aacaaaaaaa gactgctctc acgagcagcc
     3781 caaaatgaga ttatcctatt catcaacata aaaatattac aaagtgaaga actagagaaa
     3841 gacaaaaaaa gcatatcaaa tcctctcaaa aataacaagt ctttaaaaaa tgacagataa
     3901 aaatgcacaa agaactaaaa aagagaaatc gcgtaaaatc aagcattaga agctagtaac
     3961 tagctagacc aattaaaaat cacaaagtga gcgtctctat ccattggtag aggggctact
     4021 tggtctagtc gacattatgt taactagctc gataaataac gattatgttc ccaaaacaat
     4081 attttatgaa ctataacaaa aatttgttat cgcatattgt atattttaat tatataatta
     4141 taggtgtata ccacttaaat ttaacacaaa gacgtgatta ttttgaaacc caaaaagaaa
     4201 atttatctag gtctcctctt ttccttgatg attggaattt ttagtatagg aattacaaat
     4261 acctatgctt cagaaaattc taatttcaat atcaactccc aaccaattga agaacaagca
     4321 attaattttg cgaaaactga ttttgaacac tccataactt caattctttt aatggaaaat
     4381 aacaccgaaa ctgatattaa ctatactctt ggttcgccat ttaaaatgct cgatggagaa
     4441 aatacggcaa atagttctat tttctttcca gtattttcag acgctgataa tacgattaaa
     4501 tatatttata ccgtcaaaaa aaatacaaat ggagaatatt caggaagtat ctcacaattt
     4561 ttagcaaaag aacttcaaaa tctagtagat agtaatataa cagatacaac cttatttttt
     4621 caagataata gcatttatta ttccgataat ggacaaatta aactactttg gaaatcacct
     4681 ttaagtacag aaaaaaatgg tgtttcttcc tttacagaaa aaaatgatct tatatccgta
     4741 aatataaaaa ataaaattaa aaatttctct aaaaaacaaa attttcctgc ggttaaacgt
     4801 gtaaaaagtg tctcctccgg agataacttt attatgatta attggcgtat agcagaaacc
     4861 caaacaagtt ttccgtggtg tgctgcttac gtagaagctg ctatcttatg taacaaaaca
     4921 gatgtacgtg ctacttcagc tagagccatt atgcattata cctatcctaa tttaaacaac
     4981 agtcaaattg aatcaaaaag tcttaatcaa gatcaaataa taaaatatgc aaattactgt
     5041 ggtgtttatc ctaaaaaagt agcacgaata ctaaaccttt cagaggtcca aacacaacta
     5101 agaaaaggaa atgccattta tatgggaact gctggttatg gaaattatag taacagccgt
     5161 catgcacttg cattatttgg gtgggtcaat caaggtggtt atcaaactta ctatgtgtgg
     5221 aatccttggt ataactaccc aactgtaata agtgcaaatg caggaccagt tacaattcct
     5281 gtacctggag gtggttttac ttgggacaat accattacaa attggtaaat aaacccattg
     5341 ctttagttat gggtctagtg ttagttcttt tcttaactag ttgttcaaaa aaacaagaaa
     5401 tacaaaagac tgatctgatt ccttttctag gaacttggca aactaaagat cattcagaac
     5461 gtctagtaat cactaattta aatacttcaa aaaatataga acaatatacc atcttagtaa
     5521 aagagaacaa tcataaaatt caaatcattg ataaaaattt aaatataaat gaacaagttt
     5581 tcatttacaa agcaacaaat aatttactaa cactgcgctc taataaaaaa caattagatt
     5641 acacttttag tttggattct ccaaaaaagc tcgtatttat tttgacaacg aatgatggga
     5701 cagaagggac agctaaacca attgaatttt ttaaagctga ttgatgaata gttgtttgat
     5761 attacaaaac aataatttca ccgaaggtga aacaagcgaa actttacaaa aagtggctag
     5821 catttgctag ccttttttgt attgtggagc gtgggttttg agggggattc aagggggagc
     5881 ttgcgcccct ttgacaagcg gttatttgca ccaaatacgg aggtatttgg ctagcaaata
     5941 tagagcttgc caagcccttt agtaagcttt agtgagcttt acttattgta cctttttagg
     6001 tacaatggta ttgcgaacga tacgtccgta ggacgtactg aaagggtggg acttatgagc
     6061 gaacaaaatc aatatttggc tagcaatcat tctaaaaaat atacctatcg atccgagcca
     6121 aaacaaatca gttttcgagt gagcgaatcc gaatttgcaa agctgaagca gtcagctgaa
     6181 gctttgcaaa tgagtgtgcc ggctttcgtg aaagccaagg cacaaaacgc gcgtctggtg
     6241 acaccgaaag tggcgccaga cattgctcag gcaatcgcgc gcgatttagc caaagctggc
     6301 gggaatatca atcaaattgc caagtggtgc aacacacacc aacacgacgt tgatcctggc
     6361 gacgcccaac gcttatccga aaacctaaaa atcatgcaaa aggagctaca aaagatatgg
     6421 caacagttaa agtaagccga accacttctt gtagccgagc gatcaattac gccgaaccac
     6481 gagcaaccgt caaaacaggg ataaactgtg acattgatta tgccaaaagc gaaatgaaac
     6541 aaattcgcat gctgtatgga aaagacgatc gtgtacaagc acacttattg atccagtctt
     6601 ttcgaccagg agaaattacc gctgaaaaag ccaatcaact aggggcagag tatgcgaaaa
     6661 aaatagcccc tgaccaccaa atcgcgattt acacacatac agacaaagac catattcata
     6721 accacattgt cattaactcc gtcaatctgg aaactgggaa caaatttcaa gcccatggtc
     6781 aagcattttt aaacaaatgt tatgacctaa atgacgagat ttgtcttgaa catgacttaa
     6841 caatcactga acgagggaag aaacccgaaa aacgaaccat gtctgaaatc aaattaaaag
     6901 aaaaaaatga acctgtttgg aaagacgaaa ttcggtttgc gattgatcaa acaatgaaaa
     6961 acaaaaaaac tcgaacgtat aaccaatttt gtgattctct taaaacattc ggcattcatt
     7021 gcttgaatcg tgggaaaaat tttacttacg aattgatcca caaaaagaaa aaagtacgat
     7081 ccaacaaact aggaaaggat tacgaaaagg agacgatttt acatgagctg gacagacgag
     7141 aacgagccta caatcaacaa cgaaccaacg agctacgaag agctcgagct agcattcgcg
     7201 aaactgcaaa agaagtacga agagaacaac gaactcttac gactgaacaa ccggaaatta
     7261 gaccgactcg aagaaaccat gaccaacacg ttgaaagagg agcagactac caacaagaat
     7321 tatcgttcta aaacgttaca agacatagaa aatgaaataa agaaacaacg tgcttttctt
     7381 gcaccttata ccaaagaaag aatacaaact acaattgatt cacaagtcaa aaaattccac
     7441 catgcgctat taaaccagcg aaataaagaa cagaaaaaag taattatgca gcgatcaatg
     7501 gatattcttg cccccttatg ttgcttaatc atgacactgt tagccttttg gctggtatgg
     7561 ggagaataaa tagaaatggc attctattta ccctatctac tcatttttgc cagtatttta
     7621 ggaattattt ggcttagcta taaggcttat caaacaagat attcattaaa aggctctaaa
     7681 attcgattta agaggttttt attgccaggg gttatattta cattatcaat cgttttaaca
     7741 gaattaggca gaagtcgccc atcctctata ggtgggtgat gaatgccgtt cggtatgagg
     7801 gttaccgttg gtaactcgaa gaccgacaac taaggcgtat tgccttttta ttttgttcca
     7861 tgttacaatc agtctataca agaaatgagt tgatataaca tggaattaga tacaaataat
     7921 cattcagtat ttcttcttta ttaccacctt gttttagtaa cgaaatatcg tagacaagtg
     7981 attgatgatg aaatatctga ttatgccaaa actacttttg aaagaatttc agaatcgtat
     8041 cacattactt tagtggagtg gaatcatgat aaagaccacg ttcatattat gttcaaagct
     8101 caacctaaaa cagaattgac aaaattcatc aatgcctata aaagtgccag ctcaagattg
     8161 ataaaacgtg actttccaag agtcaaacaa tttctttgga aagaaatgtt ctggtctaaa
     8221 agtttttgtc ttctgacaac tggtggcgca ccaattgatg tgattaagaa gtatatccaa
     8281 aatcaaggaa acaatcataa atagaaagta ggtgaacttt atggaacaac taaaagcata
     8341 caaattcaga atctatccaa cagaggaaca agaaatcttc tttgccaaat cttttggttg
     8401 tgtccgtaag gtctacaatc taatgcttga tgaccgaaaa aaggcgtatg aagaagttaa
     8461 aaatgattct tctaaaaaaa tgacttttcc gacaccagct aaatacaaga aagaatttcc
     8521 gtttttgaaa gaaattgata gccttgcttt agccaatgcc caacttaatt tagataaagc
     8581 atacaagaat ttttttcggg ataaatccgt tggatttcca cgtttcaaaa gtaagaaaaa
     8641 tcctgttcag agttatacaa cgaataatca aaatggtaca gttgctttga ttgatagtaa
     8701 attcatcaaa gttcctaaat tgaagtcatt agtaagaatt aagcttcata gacaaccaaa
     8761 agggattatc aaatctgcta caatatcacg tcattctagc ggtaaatact atatttcttt
     8821 gttgtgtaaa gaagaaatta tcgaattacc taaaactaat tccacaattg gtattgactt
     8881 aggtattatg gactttgcga ttctttctga tggacaaaaa attgataata ataaattcac
     8941 gtccaaaatg gaaaagaaac taaagcgtga acaacgcaaa ttgtctaggc gtgctttatt
     9001 ggctaaaaat aaaggtacca atctatttga agctaaaaat tatcaaaaac aaaaacgcaa
     9061 agtagctaga ttgcatgaaa aagtaatgaa tcaacgtact gattttctga ataagttgag
     9121 tacagaaatg atcaaaaatc acgatattat ctgtattgaa gacttaaaca caaaaggtat
     9181 gttgcgaaat cataaattag ctaaaagtat ttctgatgtt tcttggtcaa agtttgtgac
     9241 taaattacaa tacaaggctg actggtatgg acgaaaaatc atcaaagtag ataaatggtt
     9301 tccgtctagt cagatttgtt cagagtgcgg acataaagat ggcaagaaat ctcttgaaat
     9361 tcgagaatgg acttgtcctg tttgtcatac tcatcatgat cgtgatatca atgctagtat
     9421 caatattctg accgaaggtc taagactaca ttctatggga ttggcttaga tccaatcatc
     9481 aagaaccgta ggaactacgg ggatagcttg gtaaataaga gacaccgctg ttagtaaaga
     9541 aatacgctat caagtatgct ctattcccaa gaagctccca cttcaagcgt taagaaccgt
     9601 caggttta
//
LOCUS       DANKKG010000103         9590 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000103 DANKKG010000000
VERSION     DANKKG010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.103"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..1132
                     /locus_tag="PW004_002117"
     CDS             <1..1132
                     /locus_tag="PW004_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2727077.1"
                     /translation="DIMFLAFSLSSMITSFHISGTQAGFISTITNLGMLIGGIFFGII
                     ADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFRFIAGVGAGGEYGACMSLISES
                     FSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGWKMLYVVGVLPVLMVLFIRRGL
                     TEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIMVTVQIAGYFGLMNWLPSIMQN
                     RLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPKLAFSLFLICSSLSVYLLVLAH
                     SQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRATANNFIMNLGRAVGGFSSIVI
                     GFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALRESLQH"
     gene            complement(1298..1720)
                     /locus_tag="PW004_002118"
     CDS             complement(1298..1720)
                     /locus_tag="PW004_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2727078.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            2115..2309
                     /locus_tag="PW004_002119"
     CDS             2115..2309
                     /locus_tag="PW004_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727079.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
     gene            2306..2752
                     /locus_tag="PW004_002120"
     CDS             2306..2752
                     /locus_tag="PW004_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727080.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLS"
     gene            complement(2962..4134)
                     /locus_tag="PW004_002121"
     CDS             complement(2962..4134)
                     /locus_tag="PW004_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL2727081.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(4340..5155)
                     /locus_tag="PW004_002122"
     CDS             complement(4340..5155)
                     /locus_tag="PW004_002122"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HDL2727082.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(5305..5751)
                     /locus_tag="PW004_002123"
     CDS             complement(5305..5751)
                     /locus_tag="PW004_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HDL2727083.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(5748..6752)
                     /locus_tag="PW004_002124"
     CDS             complement(5748..6752)
                     /locus_tag="PW004_002124"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HDL2727084.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            complement(6792..7121)
                     /locus_tag="PW004_002125"
     CDS             complement(6792..7121)
                     /locus_tag="PW004_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HDL2727085.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            complement(7247..9574)
                     /locus_tag="PW004_002126"
     CDS             complement(7247..9574)
                     /locus_tag="PW004_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL2727086.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
BASE COUNT         2622 a         1857 c         1831 g         3280 t
ORIGIN      
        1 ggatattatg tttttagctt tttcattatc ttcaatgatt acttcttttc atattagtgg
       61 tacgcaagcg ggtttcattt caacgatcac taatttgggg atgctcattg gaggtatttt
      121 ttttggcatc atagctgata aatatggacg tgtgaaagta ttttctcaaa cggtccttct
      181 gttttcgatt gcttctttat tgatgtattt tgcttcaaac atctacttgg tttatctctt
      241 tcgctttatc gcaggtgtag gagcgggagg agaatacggg gcgtgtatgt ccttgatttc
      301 cgaaagtttt tcaaaaaaac aaattggacg agcttcttcg gttgctggta ttggtgcgca
      361 ggtcggtgcg gctttggctg cgattttagc tgctgtgatc attccaacgt ttggttggaa
      421 gatgttgtat gtcgtaggtg tactgccggt gttgatggtc ttgttcattc gtcgcggctt
      481 gactgaacca aaggaattca aatctgtaca atctgccgaa aagaaaacca aacttagcag
      541 actgtttgaa tcaaaacatc ttgcttggca aacatgtggc cttagtatca tggtaactgt
      601 ccaaattgcc ggctattttg ggttaatgaa ttggctgcct tcaattatgc aaaatcgatt
      661 agggctttcg gtttctgggt cttcattgtg gatggtcagt actattttag gcatgtcttt
      721 ggggatgatg acttttggca taatcatgga taaattgggg cctaaactag ccttttcttt
      781 gtttttaatt tgttcatcct tatctgtcta tctacttgtg ttagcccata gccagtggag
      841 tttggtagca gccgctgtga tagtcggcta ctttatcaat ggcatgtatg gtggatatgg
      901 cgcagtgatt agtagtttgt atccaacaga aatccgcgcg acggcgaata atttcattat
      961 gaaccttgga cgagctgttg gtggtttttc ttctatcgtc atcggtttct tgatggatca
     1021 ttatagttta actgccgtaa tcttgttttt gagcggtatt tacctagtca gtctgctggt
     1081 cttgacaact cttactggtg ttacagcatt aagggaaagc ttgcagcatt gatagttgag
     1141 tctgtaaatg acaagaaaga ggtgtggcaa aagcttgcat ctgcggtaat aagaccagcc
     1201 aaatcaaaaa catgagacac tgtttccgtt tggctggtcg tattacttga tgcagggcag
     1261 ctttttcaac tgctaatttc cttctatctg gcggcgacta ttctttgacg tccgtcattt
     1321 caaagtactt tactaataac ttagcataac gaaaaactgt ggatacactc gtatattgag
     1381 cgtctgcata atcttccaaa tttttctcag gatgttgata catgaattca atcaaatgat
     1441 aattatatga ttttctgtag aaatgaagat aaataatgta gattgggaag ttctcctgct
     1501 tttccataga aaagcgccga ttattctcat tcttcaaaat gaccttatca ggatattttt
     1561 ggaacaattc ttctaaatct gttagataat tcaagataga cttacttgat accttcaatt
     1621 cgttttgtag ttcttctatt gaacaccaac gttctgtttc aagcagtaaa tgaatcattt
     1681 taattttctt ttgagaaact tttgttaaaa aagtctgcat attttctcct cctgtatcta
     1741 ctttactata ctacatacaa gaaaaaaaac ggatacaaat atcatattgt cacaccgtca
     1801 atagtgcata aacctgttat tatttctctt tttttcctaa gtattctatt gatatgaatc
     1861 atttaatcgt ttattatata aatgtcaata atctaataat aaaataacgt acatatttta
     1921 ttattgcata cttaataaca gaactatagc aaaaaatccc gtgtatcaac acgggattag
     1981 attaagtttc ctcaatcact attcataaca caattattat aacaagtaag taaacaattg
     2041 aaaaggacat ttattgaaaa cggttgtttt ttacttaagg aggaaattta gcgaaaaaat
     2101 tagttgcatt gtttttggtc gcaggaagtt tgttgagtgg gtgtggtgct actacgtcta
     2161 ctactcaaag cacaagttca gatcaggctt ccagcagcag taccacgatc tccgaaagta
     2221 cggcagattc atcgaaagaa caagaacaaa ttgaaaagaa aaaagaagag aaacaaaaag
     2281 cggaaaaagc tgcactagaa aaaaagtgac agaagcggaa tctgctttgt cacaagctga
     2341 agcaaatcct acaaaagaaa actatgcaac tgcttcagca gctatccaag cgctacctga
     2401 aaacaaacaa gagttgacaa gccgtcttgc tgcaattgat tccacaatca aagcaaaaga
     2461 agcagaagaa gaacggaaaa agcaagaaga aattgcacga caacaagcag aacaagcgca
     2521 acaagaagca atcgcacagc aacaagcaga acaggcgagg caagaagcaa tcgcacagca
     2581 acaagcagaa caggcgcgac agcaatcagc agctgaacag gcctctcagc aggcacaagc
     2641 cgcagctcct gagcaaaata atgaacaaac tgtctatgtc acaccgaccg gttcaaaata
     2701 ccatacacat aaatgtggaa atggtacgta tacccctgcc actttaagct aagctcaagc
     2761 tcgtggttta accccttgct ccaaatgtta tggaaactaa ttcaaaagtt atactataat
     2821 aataaaattc ttctgaaaca taaaaaaata gaagatggcg ttccagatag taaaccaaaa
     2881 ataacagttt acacgtccat ttcgtcagct tctctctttt ttcatttagt ctgttccctc
     2941 tgcttttttg gacatcttca ttcattcttt ctcaatcaaa tagtacacct ctttttcttt
     3001 tcgcagttta tatcttcctt ttttcaattt cattgaaaac tgtttcgaat gtttatgttt
     3061 tacatggatt ttaagcagga gctcctcatg ttgcaagcga tagttcctgc cttttaaatc
     3121 agaaaatctc actagtccgt attcatcaga agctttcaca aatggaactt tttctctagt
     3181 aatatagtgt ttgccgttga gcgtttttac agaaaagacc attccttcga ttggtcgtcc
     3241 gtttgtgttc tttacataaa aagataaagg atagtttcgc ttagcgatgg ccaaagaatc
     3301 taaataagcg atgagtagcc aaactaagcc gattgatcca agtagtatac cgataaacca
     3361 aataaaaaat ttccattttt tccattgatc aattccttgg atcgcttttt ttgcctcttt
     3421 tgcttgataa ggaatacgat gccccgtcac aagcagtcga tgtgtattga ccatataagg
     3481 agtacaggta agcaaagtca cgagatctct gcctttttgg atacccaaag aatctacttc
     3541 atcaggtaag accacttgga ttttttctac ttgatacgcc aatgtctttc cattgatctg
     3601 gatgaaaaac tggtctcctt ttttcaactt aggcaaatct gtaaataatt ttgcttctgg
     3661 gagtccacga tgcccagaaa gaacggcatg ggtgtttttc ccgccagtcg gataagaact
     3721 ttcttccaat aatgacgttc ctttttgcaa aaatatttcc gttgtctgat cgaatatagg
     3781 taagctgaca ttgatttttg ggatttcgat gactccaagc atatgttcca taaaaaaggc
     3841 attactaggt aaatctttta atgcttttgc atcaactgct tggttgaaac tgcctatccc
     3901 tggtgatacg ttttgctctg ccaactgttg gtttttcttt gtcatttttt cctgctgttt
     3961 ttttatttcc tctgaattct tattcgatgc ttgcttttga taatggatga tcatttgctg
     4021 ggcaagaaaa tcattcagtg aatcttctac aaaaggataa gaaaagatcc cgatcccaac
     4081 gactaaaatg attgccatga agatgtccag tattcttatt ctgcttttat tcatcatgtc
     4141 cacctatttt ctaatatgta tatgaagagg ttgtgaaatc agcgccttga cctgagtatt
     4201 agcgaagcaa acggcaaaat agttcctcct attttgccgt ttgttgagcg aataccaaga
     4261 gaccgaccac ttgtcacaac ctttctcccc atttgaatca ctatcgttta tttatcgtat
     4321 gatttttgtt tccaatagtt taagcttctg tttcttgatg gcgttttttg aaatagatca
     4381 ctgctcctcc aactaaagca gtacctacca agatgaatac gatgatcccc ataccaccag
     4441 ttgaaggcaa gattccttta ggttggttat cgatcgttgt ttcatcagaa gttgttggat
     4501 catcatctac tgtaaattca gtgtacgcag cttcattctt aggtaatgcg tagccttctg
     4561 gtgcttttgt ttcttgaagg cagtatgccc cttgtttcag cccttcgatt gaaaatttcc
     4621 cttctgaatc agaaatcaat ttcacttctt cccagtcaga acctgtgact tcatctgacc
     4681 aagcatatgg gtattctcct gaggcaaggt ttgctatttt tttcattaat ttaacagttg
     4741 gatcttttgc tcttagaata gaaaattctg catcggctaa tttttcttgc gtgttcacat
     4801 cttgtttgat gaagtggaaa tcaccgtagt tttggacatt ttctacagta atcgtgttat
     4861 ccgtagttgt attttcccga ttgactacac cgtttggatt cccaagattg gcttcatctg
     4921 cgccatggat gataccggtt gtttgttgca attttccgtt ttttactata aaagtagttt
     4981 ctttttgtgg attggttaat tgataaccat ctggtgcttt tgtttcaact aaagtataat
     5041 tgccgtctat taatccattg acactgatgg atccttcagt atcagttgtg aatgtcaacg
     5101 ctgactcagc aggatcatag agagcgacac cagtgttttc atcaaaaccg gtcacgaatt
     5161 gattctctga atttttgatc tggaattgcg cccctgctaa cttatctacc cctgaaaagt
     5221 tcccagcttt ctggattttg atattgcttt ctttgatgac atttttagca tataggtgaa
     5281 tcgtgtctaa aggtttgtca gggtctaggg taagcttaac acgatatttg gcgacgtttc
     5341 gacaccgggc tttggtgttt ctacaaagac gtatacagca tctttcccat cagatttacc
     5401 tggaagagtc aggttagcta atccttcacc agaagtcgtt acacttgcga agggtgtagc
     5461 gtctgctgga actgctgtgt ctttacttgg atagtacgta gcagtgatgt catatacgtt
     5521 gaattcaacg ccgttcaacg gttcgctatt cggaaatgtc aattccgttc cgttgtttat
     5581 ttgattttct ggtgctgaag tgaatttcaa tttatgcaaa ttaaccgtaa cattttctgg
     5641 tgtaggtgat acggctgcag aaacctctgc agttcctagg ccgagaacga aaggaagcag
     5701 catgatagca gttcctaaaa acgtccatat ttttgttttg atgtttttca ttttatttcc
     5761 ctcctattgt ttcctttttg ttgtttttta taaaaccaag ctgtccctgt tgaaaggact
     5821 agcaataacc ctaaaattga gaaacctcca agcatctctt cgcttgtttt tggaaagctt
     5881 cctccatttt tttcatttga tggaccattt gtctgcatct gaactggcgt ttttccatta
     5941 ttcgtcggat ttgcttttgg ttcgttgatg atttccaatg gtataggatg aagcggatca
     6001 taactgcctt tttcaacttc aaagagaatg gttgattgac tcaatatata tcctttaggc
     6061 ggtttaatct ccactagttc gtaacttcca aaagacaatc catggacaga aaatgttcca
     6121 tctttactgg aagtaaaaat cgttgctctt gaatcatctt tggctaccca atgatttatc
     6181 ccgttttctt gcgctaagta ttggtcattt tttttgacta gaaattgagc atctgctagt
     6241 ttttgtttcg tttgccgatc cactttgaca aattgtttgc ctcctgtttg gatggcaaga
     6301 tgtgtttcta ctgtttgatt gtcagtcgtc aaacgtacat cgttatcaaa tgtcgttttt
     6361 tcactgtttt ctttcaatgt catttggtaa cggatcgtta atttttttcc agcaaacgaa
     6421 ctaagatctt ttccagaatc tgataagaca aaaccatgtt ttattttttg tgtgtgaaat
     6481 gtatgaatct tgtctccacc aatcaaaaaa tcgatcgttt ctggttctag ccataactga
     6541 ggatcagctg tatctttgat cgtataggtt cctatctcgt tgatgttctc aggaattgtt
     6601 acggtgatga gataagggac tttatcccca tctgcaaaat ctgtctgctt attctctact
     6661 gtttttgtta atgtgagtgc agcaggtatt ttttgttcgc tctttggata caagtggatg
     6721 gtcgtcaatt tatgattaga tgaatctgtc acaggaagca caacgacaaa tggtgctgat
     6781 ttttctttta cttaatctga agtctttatt tccgtaaatc ggtaaacagc gtcacgacct
     6841 tgctcatctt tgtccgatgc actaaaagaa gcaatgccct cttcctcgtc tactgttttt
     6901 gtcacatttt ctgccagtat tttttcacta tcactttttt gtgctagttt tctttgtgcg
     6961 tcttccacac tacttccttc tgaccgcagc ttgtaaaatt catccgagac atcatatata
     7021 gcgaaagtaa cgccgttcaa cccttttact ccttgaaagt tatccgggat atttattccg
     7081 tcgttttgac tgatacttgg catcttatcg ctttcgaaca ttcgattatg caaagtaaat
     7141 gtaataggtg attctctagc tgacacagtc ggagaaccta taaaaaagac cagtaggata
     7201 aaaaagagaa cagcaaatat atccacacgt acattttttg tttttttcat cgctgttcac
     7261 ctgctttttt cttacgatgg ataaaataaa agaagccaga aaaaataaag atcagtccta
     7321 aaaagacaaa gggcagtaga cctatcccac ctgtcgaagg aagcaagaga gtcggattga
     7381 tcgtcaactt agcggtatct gttgcgataa tcgtataatt attgtttaca cttccttcgc
     7441 ctaaactaat cgttactatt tgtgatccaa tatctgtccg gctgttaact gttagggtat
     7501 aatccaatga ttcgcctaag acaacaccgg taacgtggag atttccaacc gaaatcgagt
     7561 gactttgggc atcataatta aaggcagcgt catcgacagt aatcactgcg ttagctttat
     7621 agatggtata agttcctgat gtgttggcta tatcagtaaa tgacaacttg ggatttgctg
     7681 ctttgacttt gtctattccg ctattcgtta gtttaattgt atagctgcca gcattaacca
     7741 tattttttgc tgattgtgca tctgctgtgt aatccatact gctaagtgcc acttgtactg
     7801 gagtaagcga actagtgaca ccctctgcta gcttgacatg gattgacggg acacctgctt
     7861 ttccatcata tgtcttactg ccactgagct ctaccgcacc gctgatttgc ttaatcgtat
     7921 agacatcaga tgtctgcacg atagtaaata aatagtttgt tctggcagct agtttagcta
     7981 gtccgctttt tgtcaattgc caagacgtat aggttccaat ttcagtaggc gcagttactt
     8041 caactccctt ttcattcatc gctttgaaat cagttggatt ccatgtgatt tgatcaatac
     8101 tgagatcatt gtttaaaagg gatgttgatt ctgttttatc attaaaggca tatttaacga
     8161 tcggtacaac agaaagacca tcatagtact tactgccatt tgcattgatt gatactgttg
     8221 catttgtctt ataatatagg atgacttcag aaatctgttt tccatttgcg tcctttacga
     8281 ttacatttcc atttgtatct aaactagcta aaacactcat tgctgattgg ttctttgttt
     8341 ctatcgctga aacagctgta aaggtatatc cgttgatagt aggcgtatta gtcgtcaggt
     8401 cttgtgcagc acttccgtaa gtaagattca ttattttaga aggagcaatc atctgtccaa
     8461 aatagtcttg atacttaata gtgattggtg tttgtttgcc tgtataggtc accgtatcga
     8521 cgttactttc tagggctgtg aaagaaccgt ttgtttgtgc aaaagaatag cccgttactg
     8581 ctgttggagt gataaccact ttgtcaccca catggattcc agataatgta gtcccgtacg
     8641 ttccgatttt tgctgctcca ccattgactg tataagaaaa agttcctgtc cgattcgcat
     8701 tgacctgaac cgttgctgct tgataggttg gaaaatttga aacatttccc ttaccatcaa
     8761 cgatctgccc atttttgatc gagtaccccg tgaatggaat atcatttgct aactgtatac
     8821 cgattgaaaa agtactcata cctcctccgg caggaagccc tccccatgtt gagggtgtaa
     8881 aagaataact tttatccgca ttgggtgtaa ctgaatcgat cgtatttcca gcggtagcca
     8941 cccccatata gatacttttt gggaatgtga tagtagtacc atttactgtg gagccaataa
     9001 acagattatg gatatctgga cggttatcaa taaaagcgat tgctttggtt acgccttgta
     9061 acgcagtttt atccgcactc atcgatccgt taaccgcaac aatgactcca tctttggcat
     9121 atatcactgt atatccatct ggtatggtat gtccgttgaa attcgcagtg gccgcaacag
     9181 tactaaatgt ctggcttcta cctgtttctg aataagcttt ctcccctgct gcttttgctg
     9241 ctgtgatttc tttttctgtc ggatcaatca gttgagtcga ggatgtggtt gaggttgtct
     9301 cgttgttgct agactgattg gtgctttcta tggactgtat ttcagaatag ggtggactat
     9361 ttgtctcctc tgtcgtttgg ttagcaaaat ttgacgtttc tgtataaacg tttacataaa
     9421 tacttgataa gctccctaag aaagtaaaaa tcaagatgat cgaaatcata atgggatgta
     9481 agtattttct tttagataca gctttgagtc tagaaacaaa atgtttttga catatacgtt
     9541 ggattttatt ttgagttttc ttaatcattt tcatccctcc tctcaataaa
//
LOCUS       DANKKG010000104         9581 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000104 DANKKG010000000
VERSION     DANKKG010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9581
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.104"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(<1..1031)
                     /locus_tag="PW004_002127"
     CDS             complement(<1..1031)
                     /locus_tag="PW004_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2727087.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1113..1802)
                     /locus_tag="PW004_002128"
     CDS             complement(1113..1802)
                     /locus_tag="PW004_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDL2727088.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="PW004_002129"
     CDS             complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="PW004_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HDL2727089.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2329..2832)
                     /locus_tag="PW004_002130"
     CDS             complement(2329..2832)
                     /locus_tag="PW004_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HDL2727090.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(3036..3163)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="PW004_002131"
     CDS             complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="PW004_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HDL2727091.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="PW004_002132"
     CDS             complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="PW004_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HDL2727092.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4800..5468
                     /gene="sdaAB"
                     /locus_tag="PW004_002133"
     CDS             4800..5468
                     /gene="sdaAB"
                     /locus_tag="PW004_002133"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HDL2727093.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5481..6353
                     /gene="sdaAA"
                     /locus_tag="PW004_002134"
     CDS             5481..6353
                     /gene="sdaAA"
                     /locus_tag="PW004_002134"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HDL2727094.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6444..7463)
                     /locus_tag="PW004_002135"
     CDS             complement(6444..7463)
                     /locus_tag="PW004_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDL2727095.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7636..8541)
                     /locus_tag="PW004_002136"
     CDS             complement(7636..8541)
                     /locus_tag="PW004_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL2727096.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="PW004_002137"
     CDS             complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="PW004_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HDL2727097.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9246..9416)
                     /gene="secE"
                     /locus_tag="PW004_002138"
     CDS             complement(9246..9416)
                     /gene="secE"
                     /locus_tag="PW004_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HDL2727098.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9440..>9581)
                     /gene="rpmG"
                     /locus_tag="PW004_002139"
     CDS             complement(9440..>9581)
                     /gene="rpmG"
                     /locus_tag="PW004_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDL2727099.1"
                     /translation="KAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYTLHKE
                     TK"
BASE COUNT         2705 a         2029 c         1761 g         3086 t
ORIGIN      
        1 aatacagtat tatttcggtt gcttgctgct tcacctagcc cttgtactcg tgtttgattg
       61 gcgacttgac caaattgatt cccgacgtct aataaaataa tagctaaaag caaaatggcc
      121 aaatggcttc cgccaaccaa taaagcaata aagcttattg tttgcataac aagtcccata
      181 tacacgattt ttctttcaga ataagaatct gccagtcgtc ccacgatagg tgcagcaact
      241 gctccagaca aaccaaaaat cgctaatatc cctgcttcga atgttcccca attgtaggcg
      301 ggactgctga tataaaaaat caacgtagac caaaagatcg aaaatgttcc aaacatgaaa
      361 aatccactga ctgccgcttc tcttaataga cgttgagaag caactaaatg cggcaaactt
      421 ttaagtgaat caaggtatga aacagctgta ctactttgct ggacagaacg cggcattttc
      481 cagtaaagca gaagtgtaag aattccgact gcacataaag caaataaata gatcatccgc
      541 caagaaaaaa gactggcaac aataccagat accgtccgag aaagcaaaat ccccacgagt
      601 aaaccgctaa gcaatgtacc catgaccttc cctcttgctt ttggtcccgc tagtaccgca
      661 ccgtaaggaa tgatgatttg cggcacgatc gaaagtaatc ctattaaaaa agaagcgcat
      721 gcaaataacg taaacgaagg cgcaaaaaaa gcggccaaca gagagacggc agataacgct
      781 accattcgaa tgatcaattt tggacgatcc accacgtccc ctaaaggaac taaaaacagt
      841 aaccctaacg catagcctaa ttgggtcaac atcgtcaata tcccgactgc acttgtgcta
      901 acagataaac tagctgctac ttttgttcca atcggctgga tgtaatacat attggcaaca
      961 acaacgccac aagtgattga taatagtaaa ataagcgaag ttgttaaagg tgttttttca
     1021 ttactcttca ttgatttccc ccatttccta tcttagttta caacttatgg aaaagattgg
     1081 aaatagaaaa atacaactat tcgccacact tattatttgt gcttttgttt tccttgtttt
     1141 ttcttgcgat ctttataaat gaagacaact aaccctacta gcacagtaac acttgcaacc
     1201 aacgtgactg cagttttgtt atatccgtga aacagagctt ctccaccaca cgcatacaac
     1261 acagacgaag ggatgactcc aatcacgatc gacaatatga tttgctgtag aggcaatttc
     1321 atcgcatctg cagcaaaatt gaccatagaa gatggaataa ccggtatcat atagcctaac
     1381 attacaccta tttttggatg tttcatttgt cggattgctt gcacccaatg attcgtttcg
     1441 gttttcttat ctaaaaattt taagcgatgc ataaggaaaa ttgcagtgat gttaccaaga
     1501 gcattaccca aaatgttgat gatcgcacct gtcagtggtc caaagctcaa accagcaatg
     1561 actccaacta ctgaagtagg aatacctgga acagcacaca tcaccgctgt caatacgatc
     1621 aagagtatcg atgcaaaagt cccatgcgaa cgaatatgtg cgagtagctc tgccttactc
     1681 gctctcggat ttaaaaataa ttggatgtct gtctgatatt ctaaatacaa ccgatagccc
     1741 actaccagta tcagaatgat tcccaacgag atcaacatca atcggattat tttctttttc
     1801 atatttcctc ctgaaaaaaa tctatattca ttaaataata gtttaacata aaaacaaaaa
     1861 agcaccgaaa tccaacatag gatctcagtg cttttaaata gtaaaaatta tttaacagtt
     1921 acagatgcgc caacttcttc aagtttagct ttcaattctt cagcttcttc tttagatacg
     1981 ccttctttaa ctggtgcagg agcgccatca actacagctt tagcttcttt caagcctaaa
     2041 ccagttgctt cacgaactgc tttgataact ttaacttttt ggtctccagc tgcagttaat
     2101 tctacagtga attctgtttg ttcttcagca gcagcagcgc caccagcagc agctacagct
     2161 acaggagcag cagcagatac gccaaattct tcttcaatag ctttaactag gtcgttaagt
     2221 tctaggatag ttgcttcttt taactcagca acgatgtttt caatgttcaa tgccattata
     2281 atttcctccg tttaattgtt cttttttatt taatgcttta tgcggcaatt aagctgcatc
     2341 tccatcttct ttgtctgcca ctgctttgac agcgtaagcc acgttgcgga ctggcgcttg
     2401 tagtacagat agcaacatag atagaagtcc ttcgcgattt ggcaattttg ccaatgctgt
     2461 gatttcttcc aatgaagaga ctttaccttc gatgataccg cctttgattt ctaatgcttt
     2521 tgcttcttta gcaaattcgt ccatgatttt cgctggtgcg actacatctt cgttactgaa
     2581 tgcaacagct gtaggtccag tgaatacttc gtccatgcct tcaaggccag cttttttagc
     2641 tgcacgtgaa agaatagaat ttttgataac tttcatttct acgttagcat cacgaagttg
     2701 tttacgtaaa cgtgttactt catcaactgt taatccgcgg tagtcaacga ctactactga
     2761 tgcagctgct gtaaattttt cgcttacttc atcaacgatt gctgcttttt ttgcgataat
     2821 tgcttcactc atttttgagt ttcacctcct gaattttgat ggaagagttt tttgcactga
     2881 aaaggcaagt cacttttata gttactttgc tcaaccagta ttattcatca cctattcact
     2941 tcgttcatag tgattctact cgctttgctc gaaaggaaac tcctttcgct ttgctcaacc
     3001 agtattgttc atcatctctt cacttcgttc atagtgattc acaataaaaa actctatgcc
     3061 accgcagaca tagagggact attgaatata ttcaataaac gtcctcggta ggaaattaag
     3121 gcttgatgcc acctactgtc ttcggtacaa gttaattatt aacctcaact agcttagcat
     3181 gctaaactag ttgagtcaag aattattttt cagttggatc aatcaacatt ctaatattta
     3241 attagaaaga agcttggtca acatggatac caggtccaaa tgttgttgtg atcgttacat
     3301 ttttcatgta ttgaccttta gctgctgatg gtttagcttt tagcaataca tcatggattg
     3361 ctgcaaagtt ttcaactaat ttttcatcgt caaatgaaac ttttccgatt ggtacatgga
     3421 tatttccagc tttgtccaca cggtaagtta ctttaccagc tttcacttcg tttactgctt
     3481 ttgttacgtc cattgtaact gtaccagttt tagggtttgg cattaagcct tttggtccta
     3541 atactcgtcc tagacgacct acagtagcca tcatatcagg tgttgcaaca actacatcaa
     3601 agtcaaacca tccgttttgg attttttgaa ccatgtcgtc gtcacctacg aaatctgcac
     3661 cagctgcttc agcttctttt gctttgtctc ctttagcaaa aactaaaaca gtttgtgttt
     3721 taccagttcc gtttggtaat acaacagcac cacggatttg ttggtctgct tttttagggt
     3781 ctacgttcaa acggtatgca acttcaactg ttgcatcaaa ttttgcaatg tttgtttctt
     3841 ttgctaaagc tactgcttcg gcaacaggat aaactttact tgaatcaact ttttttaatg
     3901 catcttgcat ttttttgctc tttttagcca ttttggttcc tccttgattg tggttataac
     3961 ggttgtacct cccacgtgtt ctatatcttt caatatagag ccaactgaga agctgctgtc
     4021 ttagggtgta tagattattc tacagtgatc cccatgcttc gtgcagttcc ttcaaccatg
     4081 cgcattgctg cttcaacgtt agctgcgttt aggtcttcca tttttaattc agcgatttct
     4141 cttacttgat cgctagaaac tttcgcaact ttatttttgt ttggttcgcc tgaacctttt
     4201 tcgattttag ctgctttttt caatagaaca gcagctggcg gtgtttttgt aacgaatgtg
     4261 aatgaacggt cttcgtatac agaaataaca accggaatga tcaaacctgc ttgatcagct
     4321 gtacgagcgt tgaattcttt tgtgaatccc atgatattga tacccgcttg acctagtgca
     4381 ggacctactg gtggagctgg agttgcttta cctgcaggaa tttgcaattt aacgattttt
     4441 tctactttct ttgccacgag acatacctcc ttaagtccgt gatgtggtta attggagatg
     4501 catatttctc ctcccacgtt ttatctgatc tgaaaaaatc cagacaaata atgtgcaaaa
     4561 caatgcacac tggtacagta tagcaaattt atttgttttt tcaagatttt tttctgctat
     4621 ttttacattt tgcttgaata aacgctaaat ttattctaaa aaaacaacaa tttttcgttc
     4681 tcatttcttc ttttatgtta cacttgcgct agcatggttc aacagatgaa agcaaacaat
     4741 aacacggttt tttagaccct tgtacaaaga ttcctacata acaaaatgag gtggagaaaa
     4801 tgaaagattt aaggtataga agtgtctttg atattattgg acctgtgatg attggtccaa
     4861 gtagctctca cactgccgga gctgctagaa tcgggaaaat catacgtagt atatttggcg
     4921 aacagcctga ttctgtagat atttatctat atgaatcatt tgcaaaaacc tatcgggggc
     4981 acggaacaga tattgcatta gttggcggcc tgttagggat ggaacccgat gatgaacgtt
     5041 tagctgattc tttgaaattg gcatatgaag ccggtatgga agtcttgttt gttcctaaga
     5101 gtgaaaaagc cgaacatcca aactctgtaa agatgcttct aaaaaaagga acacgaaagc
     5161 tttcagtcac tggcatttcg atcggcggtg gcaatatcca gatttctgaa ttgaatggat
     5221 tcaaaatttc tttaagcatg ggtactccta ccttgatcgt tgtccaccaa gatgttccag
     5281 gtatgattgc taaagtgact agtatacttt ctgagaccaa tacgaatatc ggcactatga
     5341 cagtgacacg ggaatcaaaa ggtgaaaatg ccattatgat aattgaagta gatgatcctc
     5401 aagtagaaga aacggtaaaa agactcaaac agctgccaaa tatcgacagc gtaaattatt
     5461 tcgattaagg agctaaaaaa atgtttttat cgattgaaga attagtagat caagcaaaag
     5521 cttatccaag tgtagcagaa ttgatgattg cagttgaaat ggaaatgagt ggccgttccc
     5581 gtagtcacat catcgagatc atggaaaaaa acctctcagt aatggaacaa tcaatcaacg
     5641 agggaaaaga aggtgttacc tccgtcacag gaattaccgg tggtgatgca aaaaaattag
     5701 atgattattt aaatgaggca gactttttaa gtggagaaac aatcttaaaa gctgtacgca
     5761 atgctgtagc tgttaatgaa gtcaatgcaa aaatggggtt gatttgtgct acaccgactg
     5821 ccggaagcgc gggtgttgtt cctggtgtgc tgatggctgt ccgagatcgg cttgatttga
     5881 cacatgaaca gcaattggaa ttcttattta ccgctggcgc tttcgggtta gtcatcgcaa
     5941 acaacgcttc tatcagtggt gcagaagggg gatgtcaagc agaagttggt tctgcaagtg
     6001 caatggcagc agcagctttg gtttgtgcaa aaaatgggac tcctcatcaa gcagcacaag
     6061 cagtagccat tacattgaaa aatatgatgg gactgatttg cgatcccgtt gcaggcttag
     6121 ttgaagtccc gtgtgtcaaa cgtaatgctt taggctcttc tcaagctttt atctctgctg
     6181 atatggcatt agcgggtatc gaaagtgtca ttccgccgga tgaagtcgtt gctgcaatgt
     6241 accaagtcgg acgtcaaatg ccgtcgattt tcaaagaaac agcagaaggt ggcttagctg
     6301 ttacgccaac tgctaaacga cttgcggaag agattttaaa taagagggcg tgataaagct
     6361 ccttaatttc gagtattaag agattagtta tcttcataac aaaaaacgaa gagaggacta
     6421 attgcccttc tcttcgtttt ttattaatcc atcaatttcg tattttttac ttcatcgatc
     6481 gtttttgttc ctgcaagttg catcgtgatc gacaattctt tgttcaggtg ttcaaaaaca
     6541 gatgttactc cttctgcccc acctagattc aacccataaa tcactggtcg gccaatcgca
     6601 actaagtcag caccacttgc cagagctttg aagacatgct ctcctcgacg gacaccagag
     6661 tcgaaaatca ctggtacacg tttgttgact acttcggcaa tttttggcaa tacttcaaag
     6721 gacgcaggac ctccatctaa ttgacgtcca ccatgattgg atacccagat accatctgcc
     6781 cctgcagaga tagcgattac tgcatcttct ggcgattgta ttccttttac gataactgga
     6841 agatgggtga tttctttgat tgttttgatg tcatctggtg tcaagccttg ttttgcagct
     6901 gcataaattt ctgcgattcc ttttccttcg ccattaccac ttgcactttg ttcgctatac
     6961 gcagctaagt taggcattgg taatggaaat tggaattgat tgatgacgtc ttcttcacgg
     7021 taaccaccga gcgttgaatc tgccgttaaa atgatagctt tagcccctgc ttctactgct
     7081 ttgttcaaaa tgaactcatt gaatctgtcg tctttactca tatataattg gaaaaactgc
     7141 ggtgcgcctg gcgctgcatc ggctgcgtct ttgattgtcg tatttgcata agtgctgatg
     7201 gaaaagattg atccagcagc agctactcct ttagctgtat cagcttcccc tttttcatgt
     7261 gccaatcctt gggccgctga tggcgcttgg ataatcggtg tatctaattc aattccgaaa
     7321 acagatgtat gcaaatcggc tgagtcgatt ccgcgtaata tccgtggcat gattttcttt
     7381 gtattaaaag aagtcgtatt ttctttcatc gtccactcat cttcagaacc accgcgaata
     7441 tagccaaaag ctcctgcttc catgcgctct tttacacgac cttctagtta ggcaatgttt
     7501 acaatatcga ttggatgttc ttcattactt gtatgataag tcatagtaga tccttctttc
     7561 atcttcttga ttacatataa atcatactaa acttacaaaa agtaagcaaa catttactct
     7621 cagatacttt tatcgctatt ctttccaaat aaatcgactt acaagctgat ctacttcttg
     7681 gttttcatga agcatactat gagaggcatt ttcacctgtc actacttttt cctctatttc
     7741 atttccgcgt tgcttcaaca acgcatagac cgctaatgca ctgtttaacg gtactgttcc
     7801 atcactcaag tctgtttcat ctagttgacc agcaatcaag aaaaatcgag tggttatagg
     7861 aatattaccg atcatttgct ggtaatcttg atagcggtta gaaacgacag caggcccctt
     7921 tgctaactca tctgtcagag actgcgcact atcatcgaca aaatcattaa aaggcgcgcc
     7981 aattgctgtt aatgtctgaa cttgtggcaa tgacgtatcc tgtccatacg ttcctaaata
     8041 gcgtagtgca ctaactcctc ccatggaatg ccccactatg ttgactttat ctatgccgta
     8101 gttttctttc agatacaaaa gacaagtttt gatccattct gcttgattcc actcgttatt
     8161 tttattatct tcaaacaata cttggaccat cggattgtct gcttttccag acaaatctcc
     8221 tgttgcttgt atttgtccat ctgttcctac tgttaaaacc aattctttta ctgcttgctg
     8281 atttttttcc aaacgccgca acatcccttt aaaggaattt tctgtcccgc tgtagccgtg
     8341 gaaaaaaagt gttggaatag tggttttttc tatcacttta ctggattctg ccgttttttc
     8401 tttatcagaa acttcagtag tatttatctt cgtcgtctgg caaccactta acgcaaacaa
     8461 actgataagg actaaagctg ctgcagttgc acgtttgcat aaatttttga ttggttctgt
     8521 catacacctc ttcctttcca tatagaaaaa agaaagactt gaaaggccaa tcatatgatc
     8581 gacatttcaa gtctctttat taatcgattt tatctacttg atcgaaatca agttctgtac
     8641 tggtttcacg tccaaacata tcgatgttga ctttcaattt ttctttttct ccgtcaactt
     8701 ctgttacttg tccttctaat ccagcaaatg cgccttcgat gattttcacg acttcgccaa
     8761 cttctacttc tagatcattt tgtcgcaggc tcataccgat agaacgcaag atatgattga
     8821 cttcttctgg aagtaatggt gctggtttgc ttccggctcc gtgtgaacca acgaatcctg
     8881 tcacaccagg tgtgttgcgg acaacatacc atgaagcgtc tgtcatgatc atttcaacaa
     8941 gtacgtatcc tgggaatgtt ttatgaacga tttctttttc tttcccattt ttgacttcag
     9001 tttctttttc ttcaggaaca acgacacgga aaataaagtc ggccattttc atactttgcg
     9061 cacgagattc gatatttgct tttactttgt tttcatatcc tgaatatgta tgcaagacat
     9121 accagttttt ttcaaaagat tccattgaag gctcctcatt tcattgtaaa ataaaaaaac
     9181 ctcagtacac ccgaagtttt tctttcgctt cattatagca ctaaaagtac gttgtggcta
     9241 gcgagttatt ttaaaatcca gccaaataag ctttggatgg ctgtatccat tacgtaaaac
     9301 atcaatccga aaataataga agtttcgatg actactaacg tatctttacg gagttgtttt
     9361 ctgcttggcc aagttacttg tttgatttca tcttttacgc tttttagaaa tttcatgaga
     9421 caacctccct tgctactgtt tatttcgttt ctttatgtaa tgtatattga ttacaatatt
     9481 tacaaaactt attgatttcc aaacgttcgc cgctttttcc ttcactgact gattttgtgt
     9541 agttgcgaga cccacaaacg gaacaagcga gtgctgcttt t
//
LOCUS       DANKKG010000105         9571 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000105 DANKKG010000000
VERSION     DANKKG010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.105"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(268..1050)
                     /locus_tag="PW004_002140"
     CDS             complement(268..1050)
                     /locus_tag="PW004_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727100.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1115..1951)
                     /locus_tag="PW004_002141"
     CDS             complement(1115..1951)
                     /locus_tag="PW004_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL2727101.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(1969..3378)
                     /locus_tag="PW004_002142"
     CDS             complement(1969..3378)
                     /locus_tag="PW004_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL2727102.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3392..5215)
                     /locus_tag="PW004_002143"
     CDS             complement(3392..5215)
                     /locus_tag="PW004_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2727103.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(6058..6384)
                     /locus_tag="PW004_002144"
     CDS             complement(6058..6384)
                     /locus_tag="PW004_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727104.1"
                     /translation="MNKVNMNKKFIIIVIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(6430..6792)
                     /locus_tag="PW004_002145"
     CDS             complement(6430..6792)
                     /locus_tag="PW004_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HDL2727105.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGADAVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVALAFIPVVFGAVAVIMGYLYRDKDEQMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(6829..7043)
                     /locus_tag="PW004_002146"
                     /pseudo
     CDS             complement(6829..7043)
                     /locus_tag="PW004_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            7171..7506
                     /locus_tag="PW004_002147"
     CDS             7171..7506
                     /locus_tag="PW004_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2727106.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(7582..9567)
                     /locus_tag="PW004_002148"
     CDS             complement(7582..9567)
                     /locus_tag="PW004_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727107.1"
                     /translation="MINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRDEFVE
                     ILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRYLSIE
                     KVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKNIPGC
                     DDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREYELVN
                     ETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNLRCLF
                     HNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLLENLD
                     LYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDDSKES
                     VTCILSGEQLAKKMSFYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLEYLNR
                     LKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEWVKIC
                     FNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELTNTVCERLR
                     NVGNNSVENALKENRGYFVKQGFTLERASKDIKRLYNVKASSSFLVYLAKGAGIDEVI
                     KELILSGVELVPLYQFTSVTRDDGRRVRKRKDPIVLN"
BASE COUNT         2960 a         2003 c         1432 g         3176 t
ORIGIN      
        1 aaaaaaacag tactgtctag tagtataaaa tatacaaatt tcttaaaaat tttttcacta
       61 aaatcactac ctgaaacagt ctaaaacact tatatatcaa cgtttaatga aactatctcc
      121 gtttcatttc agatacaaat aaatatagtc gggctccaaa tccgaaaaaa aataccccca
      181 gtagcttatc ctaagctact ggagatgtat aagtacggtc atataacaac ggggtagggt
      241 aaataaatga aaaagatgga agaatcatta ttcaataact tttttaaata cttcgttgta
      301 atcagttacc tgtccgttct taaataccac agaataatct tttttcccat tttcaagtaa
      361 ccgaagtgga atgtctttac caacttgctc tgtcactgca ttactgatac ctgttagatt
      421 agctaataaa tctgataaag attgagcatc tgtagaaatc tctgtatcag tatttatcgt
      481 gattccttta tcttcattta caagcttcca agtaggtggt atagctgtgt atgtttcgat
      541 gaatgaatca attctttcat ctatcgtctt cccagtatct acttctgtct cattactctt
      601 agatgaatcg attctttcat ccatcgtctg ctccgtaact gcttctgtct cattattctc
      661 accggaaact ctcttcacac ccaaacttat atattgtcca tctttagtgt gcatagttaa
      721 aattcccacg cttttactaa taaattcact ataaccagtt ctttcaatag agacttcaat
      781 gtatttcaca ctatcatact caaaagaaaa atagacatgt aaaagcattt tgttgtttac
      841 atctacataa taatcctttg actctacttg agaccttcct atacggaata cagctttact
      901 gtctgatatc tctataccag catccttatt attaatctgc caatctccaa gtagctcttc
      961 tggcaagcta gtagtttctt gtgaacactt taccacttca ctggtagagg tcgaaacatt
     1021 cacctccgca ctgttagaag aattttccaa ggaatcagga gccttagcac aaccaacaag
     1081 tctttatata ttgtgcagcc gatattttct tcattcagta cacccttgaa atatgcagtg
     1141 ttagataaag cagttcgtcc tgatttgctg aggcacccat ctcttcttga acgtactcct
     1201 tgattttcaa ggccgcccga tactctttcg gaaaacgctg cttcactgaa gccagcaaaa
     1261 aatcatcctt ctccggcaac tcttccttct taatcattcg ggcaataaaa aactgtaaat
     1321 gcgtcacaaa tcttaagtaa agcatcgaat ctttatcaac ctttttttcg ggaaacagat
     1381 atcctagcat agccaagata tcctttagtg ctcttaccga gagcatggtt tcttccatat
     1441 ttgcttgttc actttgggca tttacaatgt gaaacgcaat gtttccagcc tcctcttctg
     1501 gcaactgaac attcattttt ttgttgatca attggatagc atagcagccg atctcaaatt
     1561 cttttgggta atacttttga atttcaatca gcaggcgatt ttgaaaaaca atgccttttt
     1621 gactgcgttc aatggcatgc ttcaagtgat caaccaaggt aaaaaataga cttttattca
     1681 gcttaaagtc aaacttggcg ttggcataat cgataattcg cttgacgcaa ataattagat
     1741 catccggagc agcctcaaac agtttaatgt aatccttttt tagagcatca gaaagtgaat
     1801 ctaatttaaa tactttttgg attttgtcag ccggaaccaa atcgcctttc tttagttgaa
     1861 atccaatccc tttccccatc aaaattactt ctttgccttg cttatctaag caaagaatta
     1921 aactgttgtt taacgtttta attactctca ttagattcac cgttctctct aagctaagcc
     1981 atttcttgcg attacttttt gataccaaaa gaagctttct ttttttattc gttttaattc
     2041 tttaagattc tcatcggttc ggtcaacata tataaaacca taacgcttat tgaagccttg
     2101 atgggtacta acaacatcaa tcgctgacca aggtaaataa ccaaaaacat ctacaccatc
     2161 tgttaaagct gcttgaatag ccagaatatg tttggctaga taatcaattc gatcctggtc
     2221 attaattcgt ctatcttcct caactatgtc gggtgcccca tagccatttt cagtaattaa
     2281 aataggtaaa tcatagcgat cgtacaattt cctcaatgtt aatcgtagtc cagtagggtc
     2341 aactacccag ccatatggcg ttttatctac ataaggattt tcagctgccc ggtagacacc
     2401 ttcttctccc aacatgatct gttgatcccc cgctctagcg gaaacatctg atccgtcatt
     2461 tttgctggca gcgatggtgg cagttgaata ataattaatc gcaataaaat cgggtttacc
     2521 ggaggataaa attgcctgat cctctgcctc aattttagga tataatccac gatcattcaa
     2581 atagttttca aacaaataat tgtattttcc ccgaactgct acatctaaga aagaccagcc
     2641 tctcaatgtt tcccaattat gggctgcaat tgcatcttct ggactggagg tagcttgata
     2701 catagccgtt aaattgattg ctggaccgat ttttccttta agattcaact gatgatacaa
     2761 gtgaattact ctagcttgag ctaataaaac atggtgatta atttgaaaga gttctttttt
     2821 tgaaggcagc tctccacttg gtggcaaacc aatcgctcca ggatgaagga tcatcgtgtt
     2881 ttgctcatta attgtcagcc aatagttcac cttgtcacca tactcagtaa gcaatacctg
     2941 tacatagtcc acaaaataat cgatagttgc tcgatttaac caaccgccct gctcctctag
     3001 aaaataaggt aaatcgaaat ggtacatggt gacaatcggt tcgattccct gattcaggca
     3061 ttcatcaatt acttgatgat aaaaagaaat tcccttttga ttggttttat tattttcaat
     3121 atccataatt cgcgtccaag cgatggaaaa gcgaaaagct tttaagccta attctttaaa
     3181 cagctgtata tcttctttat agtgatggta aaaatccgag gcaacttcaa aattagctac
     3241 cccttctgta tgctgatact tgtcaataat agacggttgt ttcccatcct cctgataggc
     3301 accctctgcc tggtaggcac taatggaggc accccataaa aacttctctg gaaatgctga
     3361 aagctgtttg tattgcacaa aaatcctctc cttaactaat ttgcatcaat ggttcgtgtt
     3421 cctttaagct tccgcttgct tcactaattc ctgcgtaatt ttcactgttg gtcactacca
     3481 caatcactgt atcatcgtag ccagcgtttc taatggcagc gaaatcggcg ttgattaata
     3541 attgtcctgc tgacacctga tctcctgctt ttacaaatac ttcaaagggt tcccctttta
     3601 aattcacggt gtcgatcccc acatggatca ataattcaat accttcttta gaacgtagac
     3661 caatcgcatg tttagattct aaaatagtaa tgacttctcc ctcaacaggt gaataaatgc
     3721 tgttttcttc tggatgaatg gctacgccct ttcccaatag tcccttagaa aagacagcat
     3781 cgttcacttc agaaatagga cacacggttc cttttaccgg attttcaaca atgactgctt
     3841 cggctattgc cgtttgctct gattcatcag aagtatcgtc aaatccaatt agataagcaa
     3901 tgataaaggt tgcaacaaag gcgattacac acgtaataat tgcgtgaaca atattcattg
     3961 gattttctcc aataaaggct ggtaaggctg ctaagccagg ggaaacaaag gcatatctga
     4021 ctaaaccgct caagccagca tacaaaccag caattgcacc accaatcatg gaagcaacta
     4081 atggtttttt cagcttcaag gttactccat agagtgaagg ctcagtaatt cccattaaag
     4141 cagtgatccc agtggacaca gctagctgct tcaactcttt gtttttagtt ttcgcggcta
     4201 cagcaaaggt agcacctgat tgagcaatat tagaagccag cattcctggc ccattgatca
     4261 tttcagaacc cgtactgctc aattgcatag tagcgattgg cgtcattgcc caagctgtac
     4321 ccgtaaccac caaaaatggc tgcaaaccac ccatcaaaaa tggaattaac caagctgctt
     4381 tgccgttaat ccattcagcc cccattgcta taagatcgtt cagcaacgtt ccaaacggtc
     4441 caatgacaac caacgctaaa ggccccataa ttaaaaatac cagcatcggc ttagtaaaga
     4501 atttaataaa cgaaggcgaa attcgatccg caaagggctc tacataggac atgatccaaa
     4561 cagtaaagat aatcggtaaa actgatcccg cataatctgc agctaatacc ggaattccta
     4621 gaaaagaaac ggcatcgcca gtagaaaaaa gctgtgaaat atttggatga agcaaaatgc
     4681 cagcaacagt aatcgccaaa attgggttga catcaaactt tatggctgcc ccataggcta
     4741 atagtactgg cataaaataa aaggctgcat cagcaatagt attcaaaatg atgtaagttt
     4801 gactactgtc tgatagtaca ccagtcaaag tcaagacggc caaaatggct tttaccatcc
     4861 cggcacctgt tatagctgga atcatcggtg taaaggtggt agaaatgaca ctaatcaatc
     4921 ttgaaataat actttcttga ttgtctgaag aggggccctt tttaattgaa gttcccactt
     4981 ccttttgaat ttccttgaag gctgtttgga cttcattgcc aatcaccact tggtattgtc
     5041 caccgctatc caccacacta attacaccaa gtgttttttc taaggctttc gtatcggctt
     5101 tccttttatc tttgagttca aaacgcagtc tggtggcaca atgggttaag ctgaccacat
     5161 tttcttttcc cccaacctgt tgcaatacat ttcttcctac ttctctgtaa tccattattc
     5221 tctctcctta aagcagtaaa aaagaccaag tcaaaccacg ccccaatcaa cattgaagtg
     5281 ttagtttgtc ttggtcttgc ctgcataatc agtaacatgc caataacgtt attttgctgt
     5341 aatccaataa tagcacatta tgatagcgca atcaacaggg aatttaattt tttatctttt
     5401 cgtttgaccg tatagggtaa tataacagtt agatcacatc aactagggta tatctgcgta
     5461 tacttactgc taaaataagt atatctaata ttaactatac cagtaaaacc attcctacat
     5521 aaccagcggg aaattcccaa aatcgtgaaa gcatcaaaaa tgcatataac ggatcacaca
     5581 acttcaacaa ttctgctctt gggaaaaatt gatgaatttg gtgagtaaag gatggaataa
     5641 acagcattcc aacaatatag gcaacaatag tgatacttgt ttttttggta aaaatcagta
     5701 agcaatagcc aatttcaatc attccgagat ataaaggtat cgatagaatg atttgggcta
     5761 gtattcttaa aaattaacta gcaaatgttt aaactgattt atttctaatc tacatgtagt
     5821 aagtatttca attccaccaa ttatcaataa aaataccaaa catatccata ataatttttt
     5881 ctttttcatt tcttttatat tgttataaca gtgaataaca taaaaattcc cgacaatcat
     5941 tgtaatattt ttttgtcacc tgtattagta tttaatttat aggaaactat aaaaactccc
     6001 tagtgaattt acacctttgg gagttcctgt ttggacggta tgcataatac atttaaatca
     6061 atttaacatt aacacctcag catatggtat ctccacatat atacggtctt tctttggata
     6121 gtactcaata tttatgttga tagcacttcc attgtctaat atagctttag cccggtatgt
     6181 gttatctcca ttttcatgag taatcgtcac atcatcacat gatcgattta agttgtactg
     6241 ttcttgtaaa atctgtgtta ccaatggttt cgatgacttc tctaatccag atgtacctgt
     6301 catttttgaa aaaatgatac ctataatagc caagacaacg ataactataa tgataaactt
     6361 cttgttcata tttactttat tcaacttttt atctcctatc tccaaaataa tttgctctta
     6421 tccctcttat cagtacattg acatccctaa aagtacacca aagattgctc cagctacccc
     6481 tgctatcatc aaaatcgtac ccatttgttc atctttatct cgatataagt agcccataat
     6541 aacagcaaca gcaccaaata caacaggaat aaatgctaac gctactacca tagaaatcca
     6601 accaataatc aaatacagct tccctgtctc ttgtccatgt acgatttgtt ccttcatttc
     6661 attaattgtc tgctcagtac ttttctttac tgcgtctgct ccagttgtct catctgtttc
     6721 ttgtagtgct ccgcaaaact ggcaatactt actttcatct gttatttctt tgccacattt
     6781 tctacaatac atgtcttctc ctcctaaaaa cattcaattg tttattagtt aactgtttac
     6841 tttattaaat acatcagaaa acagctcatt cattggtaca cttaatatct cagaaagcat
     6901 cttcatttga tagggtttag gaatcgttct ccctgtttgc cacgaactta cacattgctg
     6961 tgtaacattc atttttttgc taactccgtt tgagaaagtc ctttttcttt tctgtatcgg
     7021 ctgagattac ttactttttt catgattctc ccctcctgtt ttattatata caacaatatg
     7081 atgtatttca actcataaaa tataaaaaaa cttcaaatac cattacaaat acaacaactt
     7141 gttgtactat actgagatgg gggtgtatta atgaaacaga aacaatatgc aaaggtcatt
     7201 agagaaaagc gaaaagctct cgggtggaca caaaaagaat tagctgaaaa aatcttctct
     7261 acacaacagg cagtagcaag atgggaaaat tctgtaacag agcctaacct cgatagctta
     7321 accgctcttt ctagagccct aggaacccct gtaagccatt ttctagacaa tgttgtcgta
     7381 gattatgagg aagagttttt ggctctctat cgttctctta gcactgaaga tgcagttcga
     7441 acaattgact atatgaagct attaaaaagg caagaaaatg aacgcaatca gctattgaaa
     7501 gattgaaaaa taaataaccc accgaacttt aacggtgggt gtattgagat gaaataagat
     7561 taagagctat ggataacctg tctagtttaa tacaattggg tcttttctct tccttactct
     7621 ccgtccatca tctcttgtta cacttgtaaa ttgataaaga ggaactaatt caactcctga
     7681 taatatcaat tcttttatta cttcatctat ccctgctcct tttgctaggt agactaaaaa
     7741 actactactt gctttcacat tgtatagtct cttaatatcc ttagaagctc gttcaagagt
     7801 gaatccttgt ttaacaaagt agcctctgtt ttcttttaaa gcattttcca cgctgttatt
     7861 cccaacatta cgtagacgtt cacaaacagt gttagtaagt tctttctctg tcattagata
     7921 tgttccttgc cacgataagc cctgtaaaac ttcatgtgct tcatctaaaa tcaattgttt
     7981 ttccttgcta agttctgatc gggaattttg aggaaatact cttttagcct ctttttcgtt
     8041 aaaacatatc ttaacccatt cataagaaaa atattgatat gtaaaatgaa atttttccag
     8101 aagtggaagg atatcttcat tcaatttttg aatcacagaa gttgcattaa aaacgtctaa
     8161 tcgatagaca ttgcttctct tatgattaaa gtttcctaac gctttctctt cctttaacct
     8221 atttaggtat tcgagcatct tcaaaggaac atcttcatca tctaattttt ttatcaatcc
     8281 tataaaagtc ataagtgata gtaacttatt acatttatct atgtcataag ttttaccata
     8341 gtaaaaactc atttttttag ctaattgttc ccccgacaaa atacaagtca cactttcctt
     8401 actatcatca aaaaaattaa catcagaaag caagacatca tagacacgtc gcatttcctc
     8461 tagataattc cttattgtcc tgaaaagttt gtctttcatt gattcaagag tgtcaaaaag
     8521 tacttttgat tcctctttca attttcgaaa atgtgtagga tacaagtcta ggttctctaa
     8581 tagccaatta aatgtttcta cccgactgtc tttctttaaa attagcatca caactccaat
     8641 aaaattttca ctgatatcac aattactgga atgacaataa aaaatggaat gcccagtttt
     8701 actggtaaaa atactagcag agggattatg atcgttgtga aacagacatc ttaagttttt
     8761 acttgactta agtaatttat tcatatcaat ggttaacaat ctttcataaa tctcattaaa
     8821 agacatgtcg gaggaatcaa acaggcagtc tttaaaccac tgtttcattt cttctttgtc
     8881 gtcatttttt atcaacttaa caaaatcggt ttcattgact agttcatact ctctccctga
     8941 atcactttga caatttttag aacatgtcat ggtaaaatca atcggaagct cagtaatatc
     9001 taacatgttg tcttcattaa ttattatcac tgcatctgac ttatatcctc caaaaaacat
     9061 tctattcgaa gcatttacat tgtcatcaca cccggggatg ttctttttca agtattcgta
     9121 aacagcaacc attttaccat agtcagtaat tgcttctttt aatagaaaga caatcttgta
     9181 tttttctaga ttttcagagt accgaatact tttttgtatc gcaaacgcag aattatgaac
     9241 taaattgata gataaaacct tttctatgct taagtatcta ctatcatctt tactcaaatc
     9301 tactaccttc cccttctcat ctttaacagt gttatcgaca tccagaataa ttactctggt
     9361 ttccacaaaa ctctccttgt cattgccttt attctttgtc ttgcttggaa taaatgattg
     9421 tgatgaattt agtatctcta caaactcatc tcttgtaact tctctatagt tatctaaaca
     9481 cctatctctt acatcagttt tttctttttt tgtaactggc ttctctaata atttttcttt
     9541 atctacataa attggaatat taatcattga t
//
LOCUS       DANKKG010000106         9570 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000106 DANKKG010000000
VERSION     DANKKG010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.106"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..818
                     /locus_tag="PW004_002149"
     CDS             <1..818
                     /locus_tag="PW004_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDL2727108.1"
                     /translation="LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYL
                     ECYFSRFSLATREAVQWIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHR
                     VKETTARLKGTSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIVD
                     RLLSACPNLRQGYQLYQDILYAVKKRDQSLFEDCLNREVSGLPETYTTTLRTFKKFLP
                     QIQNALHYSYSNGPLECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGKTFLTK"
     gene            938..1138
                     /locus_tag="PW004_002150"
     CDS             938..1138
                     /locus_tag="PW004_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL2727109.1"
                     /translation="MIEEEQKNIWDRYYKADPSSKNTKIGESGLGLSIVKELVRLHHG
                     SIEVTSRKNEGTTFLIQFPKEK"
     gene            1214..1411
                     /locus_tag="PW004_002151"
     CDS             1214..1411
                     /locus_tag="PW004_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727110.1"
                     /translation="MVECLKNKKMEANFVKAVISGSIVFQEEYRDMEKDFSKKGIELI
                     DYPRSCPILDKEYPEILTTFF"
     gene            complement(1708..3255)
                     /locus_tag="PW004_002152"
     CDS             complement(1708..3255)
                     /locus_tag="PW004_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HDL2727111.1"
                     /translation="MNITFLETSDMHGYVSPTDYSGRTDLALGTAKVSSKLKELRAKA
                     DGPVITIENGDFIQGSPLSYYLAKEYGSAKELTDLINQMSYDVQVLGNHEFNYGVDYL
                     EEAIKNYQAPILGANMVNKNDQPYFGNAYKIIEKVGVKIAILGLTTQYIPHWEKPETL
                     AGITFKSIVETAKEYVPLLRKQADLVIVSYHGGFEKDLLTGEDTEALTGENEGYELLQ
                     QVEGIDALFTGHQHRKIAETVHGVPVVQPGYRGEFIGEIRLTVEKKEGQVVVTDHQVA
                     LHSVEKSEPDPEILAAIVPVEEKLEKWLDQPLGKVNGNMRINDPMQARLEEHPYVEFI
                     NRVQLFASGSEISGTALFNNEGKGFGETITMRDVITNYIYPNTLAVIQINGADLRAAL
                     EQTANYLAVENNQIVFNPAFIQPKPQYYNYDMYEGIQYTIDMKQPVGQRITELRFKGH
                     PVLPQQMLEVVTNQYRAIGGGNYAMFGAEKIVREIQIDMTELIADYLNIHPRIEAKVN
                     HNFQVIL"
     gene            complement(3339..4142)
                     /locus_tag="PW004_002153"
     CDS             complement(3339..4142)
                     /locus_tag="PW004_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL2727112.1"
                     /translation="MNILHISDIHFRREYEACEEGYKGMLATMQNPLIPLEQCIHHLL
                     QQTPLDLVIISGDLTEDGEIDDYRYLKTWLENALGETTILVTLGNHDIKEHFWVGWCD
                     EAASSAPYNQIVKYPEFTVISFDNSSYGYADGIVDDQQFQWLKKALEQEKDQPIFLVT
                     HHHLLSHQSSIPKWPGTERFLKLIAPYDIRCILNGHTHHTFTDEINGIPYFTVSSMSF
                     VGEDEGDGFVRFEERHGYNLYHLDQGRIVRQTTENFIPGNILKTLQMKD"
     gene            complement(4145..4927)
                     /gene="phnE"
                     /locus_tag="PW004_002154"
     CDS             complement(4145..4927)
                     /gene="phnE"
                     /locus_tag="PW004_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005233297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HDL2727113.1"
                     /translation="MMLREMNRRTLLSLVALVILVILSAATLDYSGLSTFSPSMGMDV
                     LRGLGQPDWQFLYDGSGEDLISLLLLTIGIACLGTMIATVLAIPITLISAVNLWQAHP
                     WVTKIGKLICNVLRAFPELVFAIIFVKVVGPGPFAGVMAIGVHQIGMLGKLFTEEMEA
                     MDERLVEEMHAVGANFWQTIFFVRIPYLMPIYCSLALNHFEIAVRSAATLGLVGAGGI
                     GAPLIFAIQTRSWSKVSIILIGVVVTVFILDQITGIIRKRLR"
     gene            complement(4924..5703)
                     /gene="phnE"
                     /locus_tag="PW004_002155"
     CDS             complement(4924..5703)
                     /gene="phnE"
                     /locus_tag="PW004_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HDL2727114.1"
                     /translation="MSLKETVHFKWYKHLLILLFLIVCFQGSAWITSADFSQVFQNSN
                     QMTVFLSRFIHPDFSYLPKLVEPMVKTLQMSLLGTVIGLILAIPVSFLATTVVTENRL
                     LSTIFRFLLGLVRTIPTLLLAALFVAVFGIGEATGVLTIAVFTFGMVSQLMFQAIETI
                     DFGPIEAAAAVGANKMQIAIWSIAPQVLSQFASYAFYAFEVNVRASTVLGYVGAGGIG
                     VILNSSLALLNYERVSIIILTILVTVAIVDKLSERVRRSLL"
     gene            complement(5675..6466)
                     /gene="phnC"
                     /locus_tag="PW004_002156"
     CDS             complement(5675..6466)
                     /gene="phnC"
                     /locus_tag="PW004_002156"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HDL2727115.1"
                     /translation="MIIFDNVSKVYPNGVVGLKDIDLTIEDGEFVSIIGLSGAGKSTL
                     LRSINRLAETTKGDIRINGTSITKANKKELRQIRRQIGLISQSFNLVKRSTVQKNVLS
                     GRLGYYSTWKSILGLFTKEDYQQTKEALEKVGLSDKLQTRSDELSGGQQQRVSIARAL
                     VQQAPIILADEPVASLDPITTQKVMMDLKNINQTMDKTVIVNLHSVPLAREFSTRVLA
                     LKAAQVVFDGRPDDLTDERLEQIYGNAIFEEKAGEIDELERNRSL"
     gene            complement(6527..7456)
                     /locus_tag="PW004_002157"
     CDS             complement(6527..7456)
                     /locus_tag="PW004_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HDL2727116.1"
                     /translation="MQFKQLAKGFIGVMLLTTLAACGSGNTSSSSEKESKEKLVVQFV
                     PTNNDGSMEAKAKPFAKYLSKKLDREVEVTLATDYSTIVEAMSSGQVDVGIMPPAAYV
                     QAKDAGAADAILTSQLGDYDQETGLPLKDKLTNTFKGEVLVRADSGLNKLTDLKGKNI
                     ATLSPNSASGYIYPVAELKDAGIDPTADTKMTTVNDIPSEITAVLNKQMDAAFVFEGA
                     RNVFASNFPDNDLFKDLKVLYLTKGDIPNDAIAVQPDMSKDLKAKIKETFLNMKDDKD
                     GKEAMALWGHQGYEEAADSAYDTIRDYTKKAAE"
     gene            7651..8334
                     /locus_tag="PW004_002158"
     CDS             7651..8334
                     /locus_tag="PW004_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HDL2727117.1"
                     /translation="MKKIHDTKQLTAYVEKHNLQDYMDSDILAMASLLFFEKEEHLIQ
                     TGKTSDYLYFLVDGTVMVYSYSSDTQNICIDYAKPATPLGEASSLWELSPKSSVKAVT
                     KCICISIPLNQYRTQLQKDVRFLQNICQLLSYRLNSGINLANSLTEPVETRLAKFILT
                     HHENERFSFRLTTCASILNVSYRHLLRTITNFREENIIEKKKNYYLIHDIEALEDLAE
                     NISSPVKAE"
     gene            8495..9538
                     /locus_tag="PW004_002159"
     CDS             8495..9538
                     /locus_tag="PW004_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2727118.1"
                     /translation="MKKILVSLLFLVSLVTLVGCSQSQGEKQSEKEKSKIALVLGFGG
                     VDDRSFNQAGWEGLEAWGKEKKLTAKKDFTYIETNNPEDNSTNIDQAIRNGFTTIISL
                     SYLQAEELENAAKDNPKLKFALVDGEITGKKNISSATFKDQESAYLAGLAAAYTTKSD
                     QVGFIGGMEGPVIQRFQSGFEAGVKAGATALNKEITVNVQYAASYDSLDKGKAIAASM
                     YKEGVDILYQAAGSTGAGVFQEAKARNENDYSRKVWVIGVDRDQADEGDYKMENGETG
                     NFTLTSTKKNVGAEVKEFAETSHNDKFPGGKHLTFGLKEEGVDLTKGQLSEEAYQAVS
                     DAKEKIISGDFRL"
BASE COUNT         2931 a         1956 c         1900 g         2783 t
ORIGIN      
        1 ctttgccaaa ggtcttatgc tttgatgaat tcaaatcggt tcgaagtgtt gccagtgcaa
       61 tgagttttat catgatggac ggacaaagcc atgcattatt ggatattgtc gaaaatcgac
      121 gcttgcctta tttagaatgc tatttttctc ggttttcatt agcgactcgt gaagccgttc
      181 aatggattgt tattgatatg tatgcgcctt atgtttcatt ggtgaaaaag cttttcccga
      241 atgctcagtt gatcatcgac cgtttccaca tcgtccaaca tattggcaga accttcttga
      301 atcatcgagt gaaagaaacc acggcacggc ttaaaggaac ttcccactct caacgagggt
      361 tagggaaaaa attgaaacgc tattggaaac tcctacaaaa agaagaagca aagttaaact
      421 acgaaaaaag ggtttggcgt gccagtttta aagattattt gactgaaagt gagattgtcg
      481 atcgactgtt atctgcttgt cccaacctgc gccaaggcta tcagttatac caagacattc
      541 tttatgcggt aaagaaaaga gatcagtcat tatttgagga ctgtttaaac agagaagtca
      601 gcggtcttcc tgaaacctat acaaccactt tacgaacgtt taaaaagttt ctgccacaga
      661 tccaaaatgc gcttcactac agctattcaa atggaccatt ggaatgttta aacaaccaca
      721 tcaaagtatt aaaacgcaat gcgtacggct ttcgcagctt ttacaatttc aaactacgga
      781 tcatgattcg tcatggcaaa acatttttaa ccaaataaac agaacagaaa gagagtttct
      841 tctttctgtt ctgtccaaat tattctaaga tttctcttta ccaacatcat ttgacaaaga
      901 gcctgtattt attactgtcg aggacaatgg catcggcatg attgaagagg aacaaaaaaa
      961 catttgggat cgctattata aagccgatcc gtcaagtaaa aatacaaaga taggcgagtc
     1021 aggtcttgga ttatcaatcg tgaaggaact agtgcgcctg caccacggtt caattgaagt
     1081 gaccagccga aaaaatgaag gaacgacctt cctgatacaa tttccaaaag aaaaataaaa
     1141 ttaccagtaa aaaaccgtgg agagatagct gctattgatt tttgctatgt cttttctttg
     1201 agaggtatcc ggtatggtgg agtgtctgaa aaataaaaaa atggaggcca attttgtgaa
     1261 agctgttatt tctggaagta ttgttttcca agaagaatat cgagatatgg aaaaagattt
     1321 cagtaaaaaa ggcattgaat taattgatta tcctcgctca tgtccgatcc tagacaaaga
     1381 gtatccagaa atcttgacga ctttttttta aatatcgcag cagcggattt attttttgta
     1441 tttaatcaag atcggaaagg ggtattaggc tatattggtg ccgcaacttt ttcagaaatg
     1501 aagtattgtc tgatgcaaaa tcttattcat ggaaaaagga tcaaaatagt cttatttaag
     1561 gaaccatcag aggaaaattt tttgttttga tgaggtagat cgctggctaa agaatggctg
     1621 gatggaaatc agacagccta acaatttggc ataacaaagg tgtgggcaaa aaactgctca
     1681 cacctttttg aattttttat ttatatacta caaaatcact tgaaaattat gattcacttt
     1741 tgcttcgatg cgtgggtgaa tattcaaata gtcggcaatt aattcggtca tgtcgatttg
     1801 gatttctcgg acaattttct ccgcaccaaa catcgcataa tttcctccgc cgattgcccg
     1861 atattgattt gtcaccactt ccagcatttg ttgcggaaga acggggtgac ctttaaaacg
     1921 aagttcggta atccgttgtc caacaggctg tttcatatca atagtgtact gaatcccttc
     1981 atacatgtca taattgtagt actggggctt aggctgaata aaggctggat tgaatacgat
     2041 ttggttattt tctacagcta aataatttgc ggtctgctcc aacgccgctc tcaaatctgc
     2101 accatttatt tgaatcactg ccagagtatt cggataaata tagttggtga tgacgtcccg
     2161 catcgtgatc gtctcaccaa agccttttcc ttcattattg aataacgcag ttccagaaat
     2221 ctctgagccg ctggcaaata attgtacacg attaataaac tctacatagg gatgttcctc
     2281 aagacgagcc tgcatcggat cattaatacg catatttccg ttcactttac ctaagggttg
     2341 atccaaccat ttctctagtt tttcttctac cggtacgatc gctgcaagaa tttctggatc
     2401 aggctctgat ttttcaactg agtgtaaagc aacctggtga tctgtaacaa ccacttgccc
     2461 ctcttttttc tcgacggtaa gacgaatttc accgatgaac tcgccacgat agcctggttg
     2521 aacaacggga actccatgaa ccgtttcagc aattttccgg tgctgatgac cggtgaaaag
     2581 ggcgtcaatg ccttcaacct gctgcaatag ctcgtagcct tcattctctc cagttagcgc
     2641 ttctgtgtcc tcaccggtta acaagtcttt ttcaaatccg ccgtggtaac taacgattac
     2701 caagtctgct tgtttcctta atagaggaac atattctttt gctgtttcga cgatactctt
     2761 aaacgtaatt cctgctaagg tttctggctt ttcccagtga ggaatgtatt gtgtcgttaa
     2821 tcccaagata gcaattttta cacccacctt ttcgataatc ttataggcgt ttccaaagta
     2881 tggttggtca tttttgttca ccatattggc tcctaatata ggcgcttgat aattcttaat
     2941 cgcttcctcg agataatcca caccatagtt aaactcatga tttcccaaca cttgaacatc
     3001 atagctcatt tggttaatca agtctgtaag ctctttggct gaaccatatt ctttagcaag
     3061 ataataactc aaaggagacc cttggatgaa atcaccattt tcgatcgtaa ttaccggtcc
     3121 atctgctttt gcccgtaatt ccttcagttt cgacgatact ttagccgttc ctaaggccag
     3181 atcggttctg ccggaatagt ctgttggtga aacatagcca tgcatatcac ttgtctctaa
     3241 aaacgtaatg ttcattctgc gtgctccttt agctgactca aaatagaaaa accgaagcgt
     3301 attcagctgt tcggtctctt tttttgattt ataatgcctt aatccttcat ttgaagtgtt
     3361 tttaaaatgt tgcctggaat aaagttttct gttgtctggc gaacaatcct tccttgatcg
     3421 agatgataaa ggttataccc atgacgttct tcaaagcgta cgaagccatc gccttcatct
     3481 tcaccaacaa aggacatact cgatacagta aaatagggga tcccattaat ttcatcagta
     3541 aatgtgtgat gggtatgacc gtttaggata cagcgaatat cgtaaggtgc gattagcttc
     3601 aagaaacgtt cggtaccagg ccattttggt atactgcttt ggtgagacag tagatgatga
     3661 tgggtgacca agaaaatcgg ttggtctttt tcttgctcta aggctttttt cagccattga
     3721 aattgctgat cgtccactat accatctgcg tatccatagc ttgaattatc aaaggaaatc
     3781 actgtaaact caggatattt gactatctgg ttgtacgggg cagaagaggc ggcttcatcg
     3841 caccagccta cccaaaaatg ttctttgata tcgtgattgc ctagagtaac aagaatcgtc
     3901 gtttctccta gcgcattttc cagccaagtc ttcaagtatc gataatcgtc tatttcgcca
     3961 tcttcggtta aatctccgct gataatcaca aggtccaagg gtgtttgctg caggagatga
     4021 tgaatgcatt gctccagagg gataagggga ttctgcatcg tagccagcat tcccttatag
     4081 ccttcttcac aagcctcata ctctctgcga aagtgtatat ctgatatatg caatatattc
     4141 attcttatct caatctcttt cttataattc cggttatttg atctaaaata aacacggtta
     4201 cgactacgcc aatcaaaata atactcactt tgctccaaga ccgagtttga atcgcgaata
     4261 tcaatggagc accgattccg ccagcgccaa ctaagcctaa ggtagccgca ctacgaaccg
     4321 ctatttcaaa atgatttaat gctaaagaac aataaatcgg catcaaatag gggatgcgaa
     4381 caaagaaaat cgtttgccaa aaatttgctc ctaccgcatg catttcttca acaagtcgct
     4441 catccatcgc ttccatttct tctgtaaaaa gtttgccaag cattccgatc tgatggacgc
     4501 caatcgccat aacaccagca aaggggccag gtccgacgac tttcacaaaa ataattgcaa
     4561 aaactagttc aggaaaagct cgtaaaacat tacagataag cttgccaatt tttgttaccc
     4621 atggatgtgc ttgccagagg ttaaccgcac tgatcaaggt aatgggaatc gctaataccg
     4681 tagcgatcat tgtccctaaa caggcgattc caatggttaa taatagcaaa ctaatcaaat
     4741 cctcgccgct gccatcatat agaaactgcc aatcaggctg acctaaccct ctcaaaacgt
     4801 ccattcccat ggaaggagag aaggtgctta atccagagta atccagagta gcggccgaca
     4861 aaataacgag aatcactaaa gcaactaatg ataaaagggt tcttctgttc atttcacgaa
     4921 gcatcataac agggacctcc tgacacgttc acttaattta tcaacgatcg cgacagttac
     4981 taggatcgtt agaataatga ttgaaactcg ttcataattc aaaagcgcaa gtgaggaatt
     5041 tagaataacg ccaataccac cagctccgac atatccaagg acagtagaag cacgaacatt
     5101 cacctcaaac gcgtaaaaag catagctcgc aaactgactc aaaacttgtg gagcgatcga
     5161 ccaaatagcg atttgcattt tatttgctcc aacagccgca gcggcttcaa tcgggccaaa
     5221 gtcaatggtt tcaattgctt gaaacattaa ttgtgacacc ataccaaaag taaagacagc
     5281 gatagtcaac acgccagtcg cttcaccaat tccaaacaca gcaacaaaaa gcgcggctaa
     5341 aagcagggta gggatggtcc gtactaaacc taaaagaaaa cgaaagatgg tagaaagaag
     5401 acggttttct gtcactacag tggtcgctaa aaaactaaca gggatcgcta aaattaagcc
     5461 aatgaccgtt ccaagcaaag acatttgaag tgttttcacc ataggctcga ctaatttagg
     5521 taaatagcta aagtctggat gaatgaagcg tgataaaaag acagtcattt gattggagtt
     5581 ttgaaaaacc tgagaaaaat cagctgacgt gatccaggca cttccttgaa aacaaacaat
     5641 taagaacaga agaataagca ggtgtttata ccatttaaag tgaacggttt ctttcaagct
     5701 catcgatttc gcctgccttt tcttcaaaga ttgcattgcc ataaatttgc tctaagcgtt
     5761 catcggttaa gtcgtctgga cgaccatcaa agacaacctg agcagctttt aacgcgagca
     5821 cacgcgtaga aaactcacga gccagaggga ctgaatgcag attgacaatg actgttttat
     5881 ccatcgtttg attgatattt tttaggtcca tcataacctt ttgagtcgtg attggatcta
     5941 atgatgcaac gggttcatcc gctaaaataa tgggggcttg ctgcacgagt gctcgcgcaa
     6001 tggatactcg ctgttgttgc ccaccactga gctcatcact gcgtgtctgc aatttatcag
     6061 acaaaccaac tttttccagt gcttcctttg tctgctggta gtcttccttc gtaaaaagac
     6121 ctaaaatact tttccaagtg gagtaatagc ccaaacgtcc agatagcaca tttttctgaa
     6181 ccgtactacg ctttaccaga ttgaagcttt gcgagattag accaatctgg cggcgaatct
     6241 gtcgcagctc ttttttattc gcttttgtaa tagaggttcc attaatgcga atatctcctt
     6301 tggtggtttc tgccaagcga ttgatactgc gcagcagagt gctctttcca gcaccactca
     6361 accctatgat ggaaacaaat tctccatctt caattgttaa gtcaatatcc ttcaagccaa
     6421 caacgccatt gggatagacc ttcgaaacat tgtcaaaaat aatcattatc cttctccatt
     6481 tttttaagtg taagcaaaaa aaagcttgct gacacttact gatgttttat tctgctgctt
     6541 ttttcgtgta gtctctgatc gtgtcatagg cagaatcagc agcttcttca tagccctgat
     6601 gcccccacaa agccattgct tctttgccat ctttatcatc cttcatattt aagaaggtct
     6661 ctttaatttt cgcttttaaa tcttttgaca tgtcaggctg tacagcgatt gcgtcattcg
     6721 gaatatcgcc ttttgtcagg tacaggactt ttaaatcctt aaataaatca ttatctggga
     6781 aattcgaagc aaaaacattt cgcgcgcctt caaaaacaaa cgccgcatcc atttgcttgt
     6841 tcaacactgc ggtaatctca ctcggaatat cattcacagt ggtcatcttt gtatctgcag
     6901 taggatcaat cccagcatct ttcaattcag cgacaggata gatgtagccg ctagcagagt
     6961 taggacttaa ggttgcgata ttttttcctt ttaaatcagt taacttattc aatccgctat
     7021 cggcacgaac caatacttct cctttaaagg tgttcgtaag tttgtccttt aatggcaagc
     7081 cagtttcttg atcataatcg ccgagctgag aagtcaggat tgcatctgca gctccagcat
     7141 cctttgcttg aacataggct gcaggcggca taattccgac atcgacttga ccagaagaca
     7201 tcgcttcgac gatcgttgaa taatcagtag ctaacgtcac ttctacctca cgatctaact
     7261 ttttcgataa atacttagca aatggttttg cttttgcctc catactacca tcattgtttg
     7321 ttggaacgaa ttgaacaact aatttttctt tgctctcttt ttctgagctg ctacttgtat
     7381 tcccagaacc gcaggccgcc aaagtggtca ataacatgac tccgatgaac ccttttgcta
     7441 actgtttgaa ctgcattttt ctcctcctgt tggatataat ttctaatacg tctaaatcat
     7501 agcagggaat caaaatcaat gaagtgacgc acgtcctatt ttacacaact tttacataca
     7561 atttacaaac aattataaat aattattcgt attgagccga aaaacaatct aagctataat
     7621 aaagaaaaac ttcttagagg tgacgatttc atgaaaaaaa tccatgatac gaagcaatta
     7681 actgcttacg ttgagaagca caacctgcag gattatatgg attcagatat attggcaatg
     7741 gcttctttac ttttttttga aaaggaagag cacctcattc aaacgggaaa aacctcagat
     7801 tatttgtatt ttttagtgga tgggacggta atggtgtact cgtattcttc tgatacacaa
     7861 aatatttgca tcgattacgc aaagcccgct acaccactcg gcgaagcctc ttcattgtgg
     7921 gaattatcgc cgaaaagcag cgttaaagca gtgactaagt gcatctgtat cagcattccg
     7981 ctaaatcaat atcgaacaca gcttcaaaaa gatgttcgct ttttgcagaa tatttgccag
     8041 ttactaagtt atcgtttaaa ttccgggata aatttggcga actcactgac agaacctgtt
     8101 gaaactcgtt tagcaaagtt tattttgacc catcacgaaa acgaacgttt ctcatttcgg
     8161 ttaaccacct gcgcctcaat cttaaatgtc agttatcgac atcttttacg aacaatcacg
     8221 aattttcgtg aggagaatat tatcgaaaag aaaaagaatt attacttgat tcatgatatc
     8281 gaagctttag aggacttagc tgagaatata agtagtccag taaaagcaga ataacagcta
     8341 taaaaaaaca gcagtcagaa gtgattgctg gttttttgat agtggagaaa ttgggaaacg
     8401 gaaaatcttc gttgaagagt agtgttctat atattggtat gctatacttt taaaagttca
     8461 atgtgtcaag acacataaaa aggggagaaa aattttgaag aaaatacttg tttcattgtt
     8521 gtttttggtg tcattagtaa cgttagtcgg ctgtagtcag tctcaggggg agaagcaaag
     8581 tgaaaaagag aaaagtaaaa tcgctttggt tcttggattt ggcggggttg atgaccgctc
     8641 cttcaatcaa gctggctggg aaggattaga ggcttgggga aaagagaaaa agctgacagc
     8701 aaaaaaagat tttacgtata tagaaacgaa caatccagag gataacagta ccaatatcga
     8761 tcaggcaatc agaaacggct ttaccaccat tattagcttg agctacttgc aagcagagga
     8821 actggaaaat gctgctaaag ataatccaaa gttgaaattt gccttggtag atggtgagat
     8881 tactgggaaa aagaatatct catcagcaac ctttaaagat caagaaagtg cgtatttagc
     8941 aggactagct gccgcatata cgacaaaaag tgatcaagta ggctttattg gtggaatgga
     9001 agggcccgtg atccaacgtt tccaaagcgg atttgaagct ggcgttaagg caggggccac
     9061 agcactgaac aaggaaatta ctgttaacgt acaatatgct gcatcctatg attcgctaga
     9121 taaaggaaaa gctattgctg cttctatgta caaagaggga gtcgatattc tttatcaggc
     9181 ggcaggaagt actggagctg gggtgttcca agaagcaaaa gcccgcaatg aaaacgacta
     9241 tagcagaaaa gtatgggtga ttggtgtaga tcgcgatcaa gcggatgaag gggattataa
     9301 aatggagaac ggcgagacag ggaatttcac gttgacttct acaaagaaaa atgtcggtgc
     9361 agaagtcaaa gaatttgcag aaacttctca taacgataaa tttcctggcg gcaagcattt
     9421 gacctttggt ttgaaagaag aaggggtaga tctgacaaaa ggacaattat cagaggaagc
     9481 ctatcaggca gtttcagacg caaaagagaa aatcatcagt ggtgatttca gactgtagac
     9541 aaagtccatt ttatggatga tgtctacagt
//
LOCUS       DANKKG010000107         9558 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000107 DANKKG010000000
VERSION     DANKKG010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9558
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.107"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            279..1127
                     /locus_tag="PW004_002160"
     CDS             279..1127
                     /locus_tag="PW004_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727119.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(1298..1639)
                     /locus_tag="PW004_002161"
     CDS             complement(1298..1639)
                     /locus_tag="PW004_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HDL2727120.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAVEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            1725..2432
                     /locus_tag="PW004_002162"
     CDS             1725..2432
                     /locus_tag="PW004_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HDL2727121.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            2419..2742
                     /locus_tag="PW004_002163"
     CDS             2419..2742
                     /locus_tag="PW004_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDL2727122.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(2831..3868)
                     /locus_tag="PW004_002164"
     CDS             complement(2831..3868)
                     /locus_tag="PW004_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HDL2727123.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(4315..5298)
                     /locus_tag="PW004_002165"
     CDS             complement(4315..5298)
                     /locus_tag="PW004_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HDL2727124.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            complement(5714..6484)
                     /locus_tag="PW004_002166"
     CDS             complement(5714..6484)
                     /locus_tag="PW004_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2727125.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(6477..7412)
                     /locus_tag="PW004_002167"
     CDS             complement(6477..7412)
                     /locus_tag="PW004_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2727126.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(8006..9421)
                     /locus_tag="PW004_002168"
     CDS             complement(8006..9421)
                     /locus_tag="PW004_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDL2727127.1"
                     /translation="MFEVFEGINSTYSSVGSEALYQRLRSFDFGEDYQLEKLIAFYQE
                     NPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRVGLFIFLGLLPLVGVGLLLLG
                     QVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVSTISAANQLAKMTTPLQKELK
                     QQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLPFISYHFVLTKLEKHSTEAIK
                     LWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKDIYHPLLKEAVVNPVNWQKNT
                     LVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQPGHVLTSMAIEDDLFEGDSY
                     FVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERIASSASVVRWLAEYPSLAFVA
                     THDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEGPSRTRNAIALLKVLNYPKSI
                     VEQAQKESLLFDEQRQWYPFD"
BASE COUNT         2808 a         2038 c         1677 g         3035 t
ORIGIN      
        1 tctttgcgat acatcatacc tcttttatca attggaacaa ttatttattt ctaacaaaat
       61 cacttcattt ttgtcagata aataaaaaac aaaaaaagtt ccatctttaa aacacgctat
      121 tcttttactt tttctcacat tctctaagaa aagaaaagga gaatgaattt ttgacaattc
      181 gagcaaaagt gattatcaaa tatgtaaaat cagctacttt tttcttaaac gtttctttat
      241 actaaatata aataagtgtt tcatgaaagg agaactagat gaataattgg cttgagtatt
      301 ttcctgaaaa cgttttggaa cgcggttaca gctatcatct gcacggtttt gtccgtcatt
      361 tgaactatac aagcaagtac ttgtccgcaa cagtttctgg cacggaagat tataaggtag
      421 tgatcacttg ggatgaaaag accaacatga cttgtgattg tctatatgcc atagaaggaa
      481 aaaaatgcaa acacatggct gctgtcttat tcgcttacga agaacgtcca ataaaaaaat
      541 ccaactactc actttccgaa ctctcgtccc ttgtttcctc cgctagttcc tctcttgtac
      601 gggagctttt aacagagatt cttatagaac atccgcactt tatcgaacgc ttcaaagtta
      661 agatgccatt tcatgcaata aattattcag ataaattaac aactatcatc cataaatacg
      721 atcatattat caagaaaaac aaaaacagga aaacagcgaa gttcatcatg gagatgcgaa
      781 agttcatcca agaagcagtt gaatcgctta ttcagcaaaa tgcctatctt cccgcattcg
      841 aattgatcaa tgaagtgatt gctactctcg aaacattcta ttgggagcct gaggatgaac
      901 gaacccttct tctgatagag gactgttatt atttatggaa agaattatta gcagaagcac
      961 ctcatgcaga aaaaaggcaa atgttctctt ggttcgtctg ccaagtcgat catactgacg
     1021 cctcttattc aaaacgctac tctataaaaa tcctgaaaga agactttcgg gaaaaagaat
     1081 tttctaacca aaagaaaaaa atagataaaa aactaaagaa acggtaaaga aagacgattt
     1141 caatgaaaaa tagcaagtaa acgataacaa ccatcctaaa tccacaagaa atacagcaga
     1201 ccttgcttta aaaacacgaa tcatgcaaag gatgaagtaa agaagactta acgcgtaccc
     1261 aatattagtc atttgactat taggtacgcg ttttgtttta taagtcatga caaatttcct
     1321 tcgttgctgg atagattttc cggcattgtt gatagatttt atgatacttt tctacatttt
     1381 gtaagagagg ttccactgca tctgtatatt ggataaaatt ctgacaacaa ctttcaggtg
     1441 tgtcgaacca ccctaaccct agtgcagcaa tcattgttgc gcctaatgca ggtccttcct
     1501 ccacacttaa acaatagacc gttgcatcga aaatatcagc ttggatttgt agccatgcaa
     1561 gatttttcgc gcctcctccc accgaaacaa tacgtgtaaa tctgcgaccc gctagttctt
     1621 tacgtttcaa tcccaccatt gacaaatgct cattaaaaat ttagaatatt cacaagttta
     1681 acgacaatta tgaaaattta tgtcgtgcta aaggagtttt gttattggat gaaaagttag
     1741 atataaaaac tgcgatcaaa gacacgttac ctaccgtttt cggttatatc ggtattggac
     1801 ttgcatttgg tatcgttggg aaagctgccg gatttcatcc actagtcgtc acgttgatgt
     1861 ccctgctggt ctatgctggt tctgcccaat ttatcacagt cagcatgctt gctagtcaca
     1921 gcccattgct ttccatcgtt ttctcgacct ttctagtcaa ttcccgaatg attctgatga
     1981 gtatgacgat tgctccttat ttcaagaaaa atcggctgct tcaaaatctg ctgattggca
     2041 cattgctgac agatgaaagt ttcgcactgg gaatgaataa attgaactat actggacaaa
     2101 agttaaactt tcgttggatg aatacagcta attggatttc ttatttgacg tgggtgggtt
     2161 cttctcttgt tggcgcactt ttagggaatt ttattactga tccgaaaaag tttggtttgg
     2221 atttcgccat cgtcgctatg tttatcgggt tactttatct tcaagtcatt tctgatagaa
     2281 acacctctaa acgattacag ttgatcctga ttggtctcac tttgattctg gtatatgtcg
     2341 gactgatttt tatcccaagc aacttagtga ttgtagttgt aacactgatt gggtgcggat
     2401 tgggggtgtg gatcaaacat gccttctttt gagtttacat tgctgactat tttaggttgt
     2461 actttagcaa cttggctttc tagggtccta ccatttgtct tactgaaaaa attcgattta
     2521 ccgcaaccgt tgcttgagta tttaagtttt gtcccgatcg tcattatgtc tgctttatgg
     2581 ttcagcagtc tatttaccca aaatatcgga catctgccgc agatcaatct cgaaaatgct
     2641 ttggcttcag ttcctacact tttagctgct attctttcaa aaagcttatt ggtgatcgtc
     2701 ttggcaggaa ttctttccct ttcgttgatt cgcttattat aaataaaaaa acaagagctt
     2761 atggcaaatt actgtcatgc gctcttgttt ttatcaaagt caaataagaa aaaaatcggc
     2821 gtattcttgc ttatccgttg aacggaccac taacaacgac ttttcctaac atatgtcctg
     2881 tttctacgtc tttggttgct tgtttcaagt tcttcgcatt catcccatct gaatagacct
     2941 tgctcaatgt actgcggata atcccttcat ttgctaaagc agcaatgtgc gctaaagctt
     3001 ctccttgcgt ttcgatttca tagttatagt ctgttttagc gaacatatac tcccagtcga
     3061 agctgataga ttggtttttc caaacaccta tatctaacgg ctctttgatc cctacgatcg
     3121 ttcccacatg tccaaatggt cggacaaggt gcttgatttg atcaaagtag cgggtaatat
     3181 cgaataatac agcgacatac tctacttgat cgattcctaa ttcacgaaaa cttttactta
     3241 agtcgtgatg ataatcgatt ggatagtcta cacctgtttt tttcagccat tcaaaattct
     3301 tcggacttgc agtcgcgtag acttctaggc cagcccaatg cgctagttga ttcaaaatcg
     3361 aaccgacgcc cccggatccg ttgatcacta agattttttt cccgcgattg gcattttctt
     3421 ctggagttag accaaatttt tcaaataaca attcataagc ggtcaatgag gtcaacggca
     3481 aggctgcagc ttcttcatct gaaagatttt ttggtgcaag agcgacaata cgttcgtcaa
     3541 ctagttggta ttcttgattg cttcctgctc ttgttgttgt acctgcgtaa aaaacgcgat
     3601 cgccgacaga aaatttttgc acttcgtcac ctacagcaac aactttcccg actccatcaa
     3661 aacctaagat acgtaaagca tttcttatcc cgttatcttg gcgaagtttc gtatcgacag
     3721 gattaacaga tactgcgttg acttttacca gaagatcccg tgaactgggt acaggtatct
     3781 gttctttttc atcaataaag ctttttggat catcgatcgg caacccttca taaaaaccga
     3841 ctaccttcat taattccttt tctgacatac gaattcctcc ttagcttcat caagttggag
     3901 catccataca gtgttcattt caatatatat agtatgctat ttgttccacc ttttttattc
     3961 atcataatta ttctcttact taaagtaaac tatttgcact tttttggtta aaaaagaggt
     4021 tgcgacaaga cttttgtcac aacctcttat aaacggtgtt ctcctaccaa ctcttcggtc
     4081 acaagtcaaa atattcatca aacatgagaa gctgtttttt gaaattgctc cttgtgactc
     4141 agagttaaac ggcaggctca caacctctga tataaacggt gttcaatcaa ctgaccctcg
     4201 gtattagctc aaaaaacgac aaaatatgaa gagctatttt tgtttggctg ttcttattac
     4261 ttgatacagg acagcttttt cacgacctct aattcacggt gttcaagcaa gaaatcattg
     4321 ttcactgaaa agcgttggat gttctttttc aaaggtctct aagatctctc gtacttcatc
     4381 agtagatttc gtattcatca actgaactcg caagtcattt gctcctggaa atcctttgat
     4441 atagatttta aagaaacgat gtagtcctgt gatggaacgc gggatgattt ctgaataatg
     4501 atcttgcaga tctagttgca gacgaagtag tccgatcagt tctttttctg tatgttccct
     4561 cgggtctttt tcaaatgcat aaggattctt gaaaatccct cggccaatca tgatgccatc
     4621 aacgccatat ttctcggcca attcttctcc tgtctggcga tcggggatat ctccattgat
     4681 cgtgattagt gtttgtggag cgatttggtc acgtaaagca atgattttag gaatgacttc
     4741 ccaatgtgca tctactttac tcatctcttt acgcgtacgc aaatggacag aaagattggc
     4801 tatatcttga tgaagtaggt gactgatcca ttcttccatc tcagacattt ccgtatagcc
     4861 gatacgcgtc ttgacgctga caggcaaacc gccagcttta gctgcttcaa tcaattgtgc
     4921 cgctacttct ggacgaagga tcaagccact gccttttcca cgttctgcta cattagggac
     4981 tgggcagccc atgttgatat cgattccttg gaagcccatt tctgccatag caatgctcat
     5041 ttctctaaaa aattctggct tgtctcccca aatatgggca accatcggct gttcatcttc
     5101 tgtaaatgtt aatcttcctc gtacactgtc ttttccatct ggatgacaga aactgtctga
     5161 gtttgtaaac tcagtaaaga agacatccgg tgctgctgca tgctttacga catggcgaaa
     5221 tacaacatct gttacatctt ccattggtgc taaaacaaaa aaaggctttg gcagctcttt
     5281 ccaaaaatta gtagtcattt atttgttact ctccattcta tcaagcagtg aagtcgcaat
     5341 ctttctcaaa aacattgtca ttatactagg aaacaaaaaa agaagcaaag tgaacagctt
     5401 aaaagatgtt tctgattgag caaaaaaaag actgtgacaa aaatcttatc acagtccatt
     5461 ttccggaata aatggtgttc aaaagcttgc atctgcggta ataagtccaa ccaaacgaaa
     5521 aataggagga gctatttttg tttggctgtt cttattactt gatgcagaac agctttttca
     5581 caacctctcc actaaacggt gttcaatcag ctgaccctcg gtattcgctc aaaaaacaac
     5641 aaaatatgag gaactatttt cccgtttgtt tctctaatac tcaggtcaaa acgctgattt
     5701 cacaacctct tttctaactt ctcaatcctc ttcctttatt gatcaaatac caagtaacgg
     5761 cgtacaaaac aacgatgaat aacacaagta cgatcatcga tgttgctact ggcacatcga
     5821 tcgttccaag gaatccgtaa cggaaaccgg agatcatata aacgatcgga ttgacttttg
     5881 aaacagcttg ccaaaagggc ggtaacatag agatggcata gaaaacgcca cctagataag
     5941 ttaacggctg caagacaaat gtaggcacga tcgatacatc atcataagac tgagcaaaaa
     6001 tcccgttgag cagtcctgct aatgaaaaca agattgccgt catcaataat gtaatgatga
     6061 ccatgaacca tgaatacaca tgaagtggta caaagaacaa ggaaatgatc gttacgagag
     6121 agccgacgag aatactgcga cccacaccac caatcacaaa gccccaaata atcacatgcg
     6181 taggaacagg tgctaccaag atttcttcga tatttttctg aaatttttga gagaagaagg
     6241 acgaagaaac attggcatac gaactagtga tagcagacat cataatcaaa ccaggaacga
     6301 tgaattccat atatgaaaaa ccacccatgt caccgatccg cccaccgatc atttttccga
     6361 aaataacaaa atacaaggat gtcgtgatca ctggcggaac cagtgtttgg acccatatac
     6421 gcaaatagcg gttcgtttcc ttgatagcta aactttttaa tgcagtaaaa taaagattaa
     6481 acatttttct cctccgtatg attattttct tctgtgattt tcaagaacag ttcttctaaa
     6541 cgattcgact tattcctcat agaaagaact ttgattcctt gtcttgtaag ctgatcgaag
     6601 attccgttca ccccttgatt tcgttcaact tcgacagcga ttgtctgctc atcttcaaat
     6661 gtacaagcat atccttcaat cacaggtttg gtatcatatg gcgctaaatc aaagataaat
     6721 gtttcaaatt gcagttttga cagaagctct ttcatacttg tattctcgat cagttcaccc
     6781 gattggatga ttccgatgtt gcgacaaagc atttcagctt cttctagata atgcgtcgtc
     6841 aaaatgatcg ttgtaccatt ttcgttcaat tctcgtagaa actcccacat ctcgcgtctc
     6901 aactcgatgt caactcctgc tgttggttca tccaagatca gcaaacgtgg ttcatgcatc
     6961 aatgccctag caatcatcag tcttcgcttc attcccccgg aaagcatacg tgcacggaca
     7021 tttctctttt cccaaagatt ggactgcttc aaatattttt cgctgcgttt catcgcttct
     7081 ttgcgagaga caccgtagta gcctgcttga ttcacgacga tttgctggac cgtttcaaaa
     7141 gggttaaaat tgaactcttg tggaaccaat ccaatctgtt gctttgctcg aactagatcc
     7201 ttgtccagat cataaccgaa gacattcact tttccagatg ttttattgac taaagaagta
     7261 ataatcccga tcgttgtaga cttcccagct ccatttggcc ctaatagcgc atagaaatct
     7321 ccttctttca ccgaaagatc gattccccgc aatgcttcga ccccacttcc atagatcttt
     7381 ttcaaattat ttatttcaag cgcatttgcc atttatttcc tatctcctta tagacatgat
     7441 tcttaattgt atcatggaat acagcagatg aaaaaacaac ttggcttcca cacaaaaaca
     7501 cacctgctaa caaagaggag atgtgttttc tagttcatga tcatcttcga tagaataact
     7561 atctaaacaa ctctggaact tttaaaatca taaaagtacc gacagcaact actacgatga
     7621 catatagaag atccttataa ctgaggttct tgtctttttt attcatgtta agctttcctc
     7681 cgtcctttta tcgaaagctt ttttctttta ttttaaccga aataaataaa atggaaatag
     7741 ttattttcaa tttctgtttt cgctgtttgt tttacggaga actttgccat ggaacgtgcg
     7801 tctcgtcgct gatgctaagt tttttcgata aaagctgcta gaaggaagac agcgtggttt
     7861 attttgtatc gtcttttgct aaatcttcca agatcatctg gacagcagac tcgttcccat
     7921 tgaattctgc cacttttttg gcatcaatgt acttcttttg ataatcgtct ttttggaccc
     7981 acgcatcatg cttcgtttga taaagttaat caaacggata ccactgacgc tgttcatcga
     8041 acaacaaact ttctttttgg gcctgttcaa ctatggattt aggataattc aaaactttca
     8101 gcaaggcaat cgcatttcgg gtccttgaag gtccttcttt taatttgaaa tcaaaactga
     8161 ttccttttcc ttctgttact tgttcttcaa agtggacatt ctcacaataa ttctttaaaa
     8221 tctctgtcaa ttcgatatca tgtgtggcga caaatgctaa agatggatac tccgcaagcc
     8281 accgaacgac tgaagcggaa gaagcaatcc gttcgatcgt atttgttcct tttaaaatct
     8341 catctacgaa gcagtagcag cgttctttgg aagccacttg gtccagcaaa cgtttgatcg
     8401 atttgatttc tgaaacaaaa taactgtcgc cttcaaataa atcatcttcg attgccatgg
     8461 aagtcaacac atgtccaggt tgcagggtaa actgttctgc aatcgctgtt tggatcgtct
     8521 gtgctagaat acagctgatg gctatacttt tgacatacgt agattttcct gatgcattgg
     8581 aacccgtgac taatgtattc ttctgccagt tgactggatt aactaccgct tcttttaaca
     8641 atggatgata gatatctttc gctgtcacac cgccctcagt aaactctggt cggcaagtga
     8701 tcggcatata ggtgcgataa ttcaaaacgg ctgctgcaac ttccaattct cccagcaatt
     8761 cccacagctt gatggcttca gttgaatgct tttctaattt agtgagaacg aaatgatagg
     8821 agataaatgg cagcatgaac atgatgttga catattcaaa aatcaattct gcttcactcc
     8881 cgctcttcat tcgaaatgaa aaacctaatt tagggatgtc tcttaatggt ttagtttgct
     8941 gtttcagctc tttttgaagt ggtgtcgtca tttttgctaa ctgattggcg gctgaaatcg
     9001 ttgaaaccaa ataactcatg ctattcagct ctgtttctag tgtttgtttc ttcactagat
     9061 aatagaccgt attgaatact aagccaccga ttgctagata gattcctagt acttgcccta
     9121 aaagaagcaa acctacaccc accagcggaa gcaagccaag aaaaataaat aaacctacgc
     9181 gtccaatctt tttacttttt ccatccgcta gatattgttt tgcaaaatta tgatcttttt
     9241 ttcctaaacg agcgaattga tattggattc tttctctcag ttgaggattt tcctgataaa
     9301 aagcaattag tttttctaac tgataatctt ctccaaaatc aaagctacgt aaacgctgat
     9361 acaacgcttc tgaacctaca ctagaatagg tactgttgat cccttcaaat acttcgaaca
     9421 tatccaagtc ataccaagta agatcgtcga tttcactatc ccatgaacgg aattttttct
     9481 ccgtgagcca cgcatccttc aaactttctt ctttatcaaa tctcggttga taaggaattt
     9541 ttccccaatt actacgaa
//
LOCUS       DANKKG010000108         9551 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000108 DANKKG010000000
VERSION     DANKKG010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.108"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            175..1434
                     /gene="lysA"
                     /locus_tag="PW004_002169"
     CDS             175..1434
                     /gene="lysA"
                     /locus_tag="PW004_002169"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HDL2727128.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1421..2221
                     /gene="dapF"
                     /locus_tag="PW004_002170"
     CDS             1421..2221
                     /gene="dapF"
                     /locus_tag="PW004_002170"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HDL2727129.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2224..3105
                     /locus_tag="PW004_002171"
     CDS             2224..3105
                     /locus_tag="PW004_002171"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDL2727130.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3102..3818
                     /locus_tag="PW004_002172"
     CDS             3102..3818
                     /locus_tag="PW004_002172"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDL2727131.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3824..4519
                     /gene="dapD"
                     /locus_tag="PW004_002173"
     CDS             3824..4519
                     /gene="dapD"
                     /locus_tag="PW004_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HDL2727132.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4535..5536
                     /locus_tag="PW004_002174"
     CDS             4535..5536
                     /locus_tag="PW004_002174"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDL2727133.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5533..6894
                     /locus_tag="PW004_002175"
     CDS             5533..6894
                     /locus_tag="PW004_002175"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HDL2727134.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6854..8005
                     /locus_tag="PW004_002176"
     CDS             6854..8005
                     /locus_tag="PW004_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDL2727135.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7980..9149
                     /locus_tag="PW004_002177"
     CDS             7980..9149
                     /locus_tag="PW004_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDL2727136.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9324..>9551
                     /locus_tag="PW004_002178"
     CDS             9324..>9551
                     /locus_tag="PW004_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL2727137.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVH"
BASE COUNT         3138 a         1660 c         2146 g         2607 t
ORIGIN      
        1 atagttcggg tattttgttt ttataaccat ttttattctt atttttaaaa aaatatttcg
       61 aaatatacta gacaattcag aaaattttgt ttaatattat tccaataaaa ttcaaatctt
      121 tatcaaatga aattccaata aaaacggaaa aaaagtgaaa agaggaatga aaaaatgaca
      181 gaattgataa ttggcggagt cacagcaagc acattggcag aaaagtataa gacacctctg
      241 tatgtgtatg atgaagaaaa gatgagacaa acgatgctgt cattcaagcg aggctttgta
      301 tcgaaacgtt ttgatacaaa agtgctttac gcctctaaag cattccaaac gattgagatg
      361 ctgcatttag ttcgtgaata tggattcggg ctagatgttg tcagtggtgg ggaaatctat
      421 accgctttgc aagctgattt tcctagagat actatctact tccatggtaa caacaagacg
      481 ccagaggaac tgatctatgc acttgaaaat gatttgttgc atattgttgc cgataatttg
      541 atggaagttg aattattagc agaattaagc caacgctatt taaaaaaaat ctgtatcatg
      601 cttcggttga atgtcggagt agaagcacat actcatgaat atatcgtcac tgcgcatatt
      661 gattcaaaat tcggcatgag ctatgaaagt aaagagtgcc aacaagccct tcagttgata
      721 aaagaaagca gctatctaga gttagaagga tttcatgctc atatcgggtc acaaattttt
      781 gatgttactg cttggttagc tgaaatcgac aaattgacga attacttaac cgattttgac
      841 caacctcttt ccttgaatct tggcggaggg tttgggattc gttatacaca aaatgatcag
      901 ccgatcccag tcgaagaggc actaaaaaaa ctgattgaat atacggaaga agctttggaa
      961 aaacgaaaac tgaccattcg tcaattactg atcgaaccgg gtagaagtat tgttggagaa
     1021 gctggtacaa cactatacac gattggtttc atcaaaaaga caccacataa aaaatattac
     1081 tttgtagatg gcgggatgac agataatatc cgtccagctc tgtatcaggc agaatacgat
     1141 tgtgatttag cagcaaaact gtcggaagaa aaaacagaga aagtgacgat tgcaggaaaa
     1201 atgtgtgaat caggcgatgt ggtaattaaa gaagcttatt tgccgaaagc agaatcgggt
     1261 gatcttttag ctgtttattc cacgggagct tatggatact cgatgagcag caactacaat
     1321 cgagcgacac gaccagcagt tgtttttgtc aataatggcc aatcaagact gattgttcgc
     1381 agacaagcat tttctgatct actaaggggg gaagtttcct atgatccttc ataaatataa
     1441 tggctgcgga aatgatttta tcttattaga ttacgcagaa gacacagatt atagtcaatt
     1501 agccgcttct ttatgcacaa aagaaaaata tgatatagac ggattgatcg ccgtaaaaat
     1561 cgatccgttg gaaatgatct attacaacaa agatggcagc cgcgcgccga tgtgcggaaa
     1621 tggcattcgc tgttttgctc gatacgtaaa tgatcagaac atgatgaatg aacaggaatt
     1681 caacgtagag acattagcag gcacaagaaa aattacggtg ttggatccct caccttttta
     1741 ttgcagcgta gatatgggag tgccagatga ttctccggaa aaaatcaaag ctaatcaaac
     1801 ttatccaata aaaaaccagg aaatagagat caatggtgaa aaagtaacga tcactagttt
     1861 atttttagga acgattcata ccgttgtttt tgttgaaaat gcgcgtgagg aagtctggag
     1921 aaaaagaggt gaactgatct gtcatcatcc tttgttcaaa gaaaaaacca atgtcaactt
     1981 tgtagaagta gtcaatgaac aagaattgat cgtccgtacc tatgaaagag gagtaggttg
     2041 gacacttgct tgcggtacag gttgctgtgc ctcttatgtg gttgctaagg aaactgggaa
     2101 aatttcagca gatcaggtag ttgttcactt ggaacaagga gacttgatga tctctggtga
     2161 ggatgcgata caaatggctg gcccagcagt tcatgaatgg acaagaatat tggaggaatg
     2221 aagatgataa aaggttcgat cgtggcgctg atcacgccga tgaatgaaga gggatcagta
     2281 gattatgctg gtttggaaaa attgatccaa ttccatttag atgaacaaac agatggatta
     2341 ttagttttag gtacaacagg agaatcttct acgctcacac aatctgagga ggagcagatc
     2401 cttcaactga cagtaaagaa agtggctggc cgtgtgccgg tgattgcagg agctggaacg
     2461 aataatacga aagaaacgat tgaaaaagcg aagcattttg cttctttggg ggcagacgca
     2521 cttttggtca tcacccctta ctacaacaaa acaagtgatg caggactagc tgctcatttt
     2581 acagcaattg cagaagcctc accgattccg cttattttgt ataatgttcc ttctagaaca
     2641 ggcatgtcga ttccaatcca tgtattagta aatttagcag aacatcctaa tatcattggt
     2701 ttgaaagaag cctcaggaga tatggcttat gtgatggatg ccgctcggtt gattggagaa
     2761 gaattcttcc tttacagtgg aaatgatgat ttgatcctac cagtaatgag tgtaggagga
     2821 agcggtgtaa taagtgtatg ggcgaatatc caacctaaaa tagtccatga attagtaaaa
     2881 gatacgcagg acggaagatg gcagcaagca aaagaaaagc aattaaatgc acttgaactg
     2941 atccatgcct tgttcagtga gaccaatcct attcctgtca aagcagccat gtcattatta
     3001 gacttacctt ccggacctct acgcctgcct ttagtttctc tttcaaaaga gaaaaagaaa
     3061 caattagctc agcttttgtt aaaagaaagg acggttgaaa aatgaaaatc ggactgattg
     3121 gcagtggaca gatgggaaat agagtcgaag aagttgcaca agaaagagga gatacggtcc
     3181 ttcttttacg gacggcacct aaagaagcaa ctacgcttgc ttttacggaa ctgccagaac
     3241 tgcttattga tttttctcat ccagataatt tagagaaaat cctttcgtat agcttatcat
     3301 atcaacttcc gttagtcatt ggcacgacag gttatacaca atcacagttc caagaaataa
     3361 aagaacatgc caaacatgta cctgtgatgt atagtgctaa tttttcgttc ggcatcatgg
     3421 tgatgaatca actaatcaag caagcagctg caatgttgca aggctggcag atcgaactga
     3481 ttgaaaaaca tcatagtcgt aaaaaagatg caccatctgg tactgcaaat atgctgcttc
     3541 aaacaatcca agaagtgcaa aagctgcagc ccgtatacaa ctggcaaggg aaaaaaagag
     3601 aagaaaacca aattggtgta cacagtattc gtgcaggttc tttacctggt gaacatgatg
     3661 tattgtttgc tgctactgat gaaatattta cgatcaaaca tgaatctttt tctaatcgaa
     3721 tctttgctgc aggcgcagta gaagcagcgg actggttgaa agatcagtca ccaggttatt
     3781 acaaattaga agatttatta atggacaaag gggtgtaaaa aacgtggaag ctcaagaaat
     3841 catccgctac attcaaacgg catctaaaaa gacgcctgtg aaagtctatc tgaatgtaac
     3901 cgagccaatc gaatttaaag aaagtaaagt ttttggtgaa ggaagcagct tcgttgtttt
     3961 tggtgactat gagacgattg ctccggtttt agaagctgaa tcagaaaaaa tcattgaaat
     4021 cgaaatcgaa actgctgcaa gatacagcgc agttcctttg ctagatatta aaaaaattaa
     4081 tgctcgtatc gaaccaggtg ctattatcag agatcaagtg tcaatcggca acaatgccgt
     4141 gatcatgatg ggagcgatta tcaatattgg tgcagtaatc ggtgaaaaca cgatgattga
     4201 tatgggtgct gtcttaggtg gacgagcgac agtgggcaaa aactgccata ttggtgcagg
     4261 agctgtctta gcaggtgtga tcgaaccagc atcggccaaa ccggttatcg tagaagacgg
     4321 cgtactggta ggtgcgaatg cagtgattgt tgaaggtgtc catatcggaa aagatgcagt
     4381 cgtggcagca ggagcagtgg ttttagaaga tgtggcagca gaaactgttg tcggtggaat
     4441 acctgcacgt gtattaaaaa taagtgatga tcagacaaaa gaaaatacag cattgattgc
     4501 tgcgttacgc gaattataga ggagatggag aaaaatgcgc gaaatagata cctgtattgt
     4561 gggggcaacc ggattagtcg gccaaacgat gctcaaagtg ttagaagaat atgattttcc
     4621 agtaggacga ttgaaattat tagcttcaaa acgttcagcc ggtaaagttt gtgtattcaa
     4681 gaaaaagaaa cacactgtac aggaattgac agaaacttca tttgaaggat atgatttagc
     4741 gctattttct gcaggtgctt ctgtatccaa agagtttgcg ccaatcgcac gaagtcaggg
     4801 actatttgtc atagataatt cctcagcatg gcgggaagat tcggatattc cattaatcgt
     4861 tccagaggtc aatctggaag actatacatt gaatcggctg attgcaaatc caaattgttc
     4921 aaccattcaa gcggtacttc cgcttaaagc tttacaagaa gcctttggta tccagcgagt
     4981 caattattcg acctatcaag cagtttctgg ttccgggcaa aaaggcattg aagatcttca
     5041 tcgtacgcaa aatgacgagg aaccagtttt gtatccttat aatattagcc aaacggtgat
     5101 tccggaaatc gatcagcctt tggaaaatgg gtatacaaaa gaagaacaga aaatgataaa
     5161 tgagacccgt aagattctgc atcagcctgc tttgccagta tcagcaacct gcgtacgtgt
     5221 gccaatcgaa aatggacatg gagtctctgt ggcagttcaa ctgcaaaaag agtttacgac
     5281 ggaagaaatt agagagtgtc tcagccagtt tccagggatt caattagaag atgatctagc
     5341 agcacataaa tatccgacga gcattcttgc tagaggaaca gatatggttt atgttgggag
     5401 gatcagaaaa gacacttctt tagagaacgg attgttgttc tatacgactg ccgataacat
     5461 acgcaaagga gccgctgcga atgccgtaca gattgctgca gctttaatgc agaaaatagc
     5521 ggaggtggca agatgagtaa tacgctgaaa gtagcgaaat tcggcggaac atcgatgtct
     5581 catgaagagc aattcaaaaa agtccgtaca attgttcttt cagatcccaa tcgccaagtg
     5641 atcgttgttt ctgctttagg caaacgtacg tcagatgatg acaaagtaac agacttactt
     5701 tatttgattt atgcttacct tcagcatcat gttgattgga cacccctttg gaaaaggatt
     5761 tgtgatcgtt tcattcaagt aagagatgtt cttgccctgc agttacctat tgaagatgaa
     5821 ctagcacgca tacagaaaca actggaagac gaaaagataa ctgaagatta tttagtaagt
     5881 agaggtgaat atctgactgc tcaactgatg gctgagtatc ttggttacca gtttattgac
     5941 gcaaaagacc tgattttctt tgactatgat gggaaaatcg atcaggaaaa aacagcacag
     6001 gaaatcacaa aagcatttaa aagatatccg aaaatggttg ttccagggtt ttacggggcg
     6061 aaccctgcag gaaaagaaaa attattgggc cgtgggggaa gcgacattac cggcgcgata
     6121 ctggcgaaga tgttgcaagc ggaaagttat gagaattgga cggatgtttc aggaatcatg
     6181 atggctgatc cgcgaatcat cgatcatcct aaaatcatcg aggaaatctc ttttagagaa
     6241 ctgagagaga tggcttatat gggggctaac gttcttcatg aagcagctgt tttcccagtt
     6301 caagaggctg atatccccat ccaaataaaa aataccaatg atcctttcgc tcaagggaca
     6361 aaaatatcga atcacgaaat agaaaaggaa aatggactga caggaatagc tggacgcaag
     6421 gattttcttt ccatcacttt attcaaacgt catatgtccg atgaaatcgg gtttatctgg
     6481 aaagcaatga gcatttttgc taaacatgag atatcaatcg aacatattcc ttcagggatc
     6541 gacaatatcg gagtgatcgt ctcagcagaa gcgattgccg ataagttatt tctcatcacg
     6601 aaagaattaa aagaaacact tggcgtggaa gaaatcgtcg tgatcgaaga cttagctttg
     6661 atctctgtcg ttgggggtcc gcataaagaa ttgattggat tatctgggaa agtgttatcg
     6721 atattgaaca aattagaaat tcgcacatcg atcttatcac aaggagcaca ggagttgaat
     6781 ctgatcatcg gtgtaccgaa taatcaatat gaaacagtag tgaagggtat ctatgaaggg
     6841 atggtgaata cggatgctga aagaatatcg aatggcactc catcaaattc ctgaaatcgg
     6901 gtttcatgaa ttcaagacaa aaaaatattt atatgaacaa gtaaaaaaca gcgaggccgt
     6961 tatccatgaa atcggcgaaa caggactact cctttatttt gataatcagc aagaagaaac
     7021 gattgctttt cgaacagata tcgatgcact gccagtcaaa gaagaaacga atctctcttt
     7081 tgcttctaag catgaaggtt atatgcatgc atgtggtcat gacggacata tggcgatgct
     7141 tttagggttg actgacttta taactaaaca cattgaggat cttatttata atgtagtact
     7201 gatctatcag ccatcagaag aaatttccgg tggcgcacaa agcgttattc agtcaggatt
     7261 gttggataaa tatgaagtac aagcaatatt tggctttcat ctatggcctg gactagctaa
     7321 gggagaagtt ttctctcgac caggaccttt gatggcacaa agcagtgaaa cagacattat
     7381 cattaaaggc cagtctgcac atattgcttc tagtgaaaaa ggaatcgata gtctggaggc
     7441 agctgtacga tttttgaaga atgtttatga ttttgaccgg caattaccgg cagagtggaa
     7501 tcatctaatg aaatttggcc aaatccaagg tggaacgatc cgaaatgttt tagctggaga
     7561 agttattgta tccggaagta tccgttctta cagttcagat atgcaaaatt atttgaagag
     7621 gggattagac catttagcag aacagtttca ctctacagct actatttctt ttcgctataa
     7681 tgacggctat ccagctgtaa tcaaccatag aggcttatat gcagcactgt tagattcaaa
     7741 caaactacat gaattagatg ctccagttct tcaagccgaa gattttggca tatatacgca
     7801 acaatatcca tgtgtatttt tctttttagg tgtcggtgat acccctgctt tacatgatcc
     7861 gacgttcgat tttgatatgg atgttttaga aaatggtgta gaatggtaca aaacgattct
     7921 ttttacagaa aatcttcttg taaaaatcga tagcagttca aaagaggagc gagtaaataa
     7981 tgatgaattt atccaatcga tttagtccta ggctttctag aatagaagta tcaaaaatac
     8041 gacaattcga tcagcaaata tcgtcgatac cagatgtgat caaactcacc ctgggagaac
     8101 cggattttcc aactcctgaa catgtcaagc aagcaggaat agcagcgatt gaagaagact
     8161 tttctcacta taccggaatg agaggattag aggaactgcg ggaagcggct tgtatatttc
     8221 agcaacagcg ttatggatta acttatgatc cgcaaacgga agttttgacg acagttggtg
     8281 caacggaagc gattgcctct gcgttgttat ctgttttgga agaaggagac aaggttctta
     8341 ttccagcgcc tgcttattca ggatatcagc cattagtgga actagctggt gctgaattga
     8401 tcccgataga tacttctgac acagggtttg tctgtcagcc ggaacaattt gaacgagcat
     8461 ttgaacaata tggatcagct gtcaaagcag tcattttaaa ctatcctaac aatcctactg
     8521 ggacgacatt gtctgcaaaa caattgaaag caatcgctga ggttttaaaa aaatatccgg
     8581 tatttgttat aagtgatgaa gtatatgctg aattgactta tagtggaacg catatgtcca
     8641 tagctactta tcttccagaa caaacaatcg tgataagcgg gttatccaag tcacatgcga
     8701 tgactggttg gcgagtgggc tttatttttg ctcaaaaagc ccttattgac gaactaatta
     8761 aagtccatca atatctggtg actgccgcta caacgatgtc gcaaaaagct gcggctgaag
     8821 cgctaataaa tggggtgaaa gatagcgaga atatgaaaga gcaatatcaa acacgaagag
     8881 actatctggt ccagcaattg gcgcctttag gatttgaagt gacccagcca aacggtgcat
     8941 tttacttgtt ttgcaaactc ccagctgcca ttcaaactga ctcttggcag ttttgtgtag
     9001 aattagcgga acaggcaaaa gttgcgtgta tcccaggaat tgcatttggc ccagaaggag
     9061 aaggttatat ccggatttct tatgctagca gcatggaaaa attacatgag gcctgcaaac
     9121 ggatcaaaga ctttttaaca gaacagtgat tcatagattt tatcaaaaaa acaatcgtta
     9181 attttgcggt atattctgca aggagacgat tgttttttaa atttagttca aatgaacgcg
     9241 tttcttttga ttcataacta atgtaatgtt aagtttaaat tgtttagaat gattttaaaa
     9301 gaatattcaa ggaggtttca tcaatggaaa aaatcaaagt tgtcattgta ggtgcatctc
     9361 atggagggca tcaatcgatc ttggaactat tgacacgata cggtgaaaat gtagatatca
     9421 cgctatttga agcaggagac tatatttcct tcatgtcatg tggtatggag ctgtatttag
     9481 aagacaaggt aacagatgta aatgatgtac gtaatttcag accagaaaac tttccgcaat
     9541 ccaatgttca t
//
LOCUS       DANKKG010000109         9447 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000109 DANKKG010000000
VERSION     DANKKG010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9447
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.109"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            2..74
                     /locus_tag="PW004_002179"
     tRNA            2..74
                     /locus_tag="PW004_002179"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Asp,seq:gtc)
     gene            78..150
                     /locus_tag="PW004_002180"
     tRNA            78..150
                     /locus_tag="PW004_002180"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:111..113,aa:Phe,seq:gaa)
     gene            155..225
                     /locus_tag="PW004_002181"
     tRNA            155..225
                     /locus_tag="PW004_002181"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:187..189,aa:Gly,seq:tcc)
     gene            238..311
                     /locus_tag="PW004_002182"
     tRNA            238..311
                     /locus_tag="PW004_002182"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:272..274,aa:Ile,seq:gat)
     gene            323..394
                     /locus_tag="PW004_002183"
     tRNA            323..394
                     /locus_tag="PW004_002183"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:356..358,aa:Glu,seq:ttc)
     regulatory      583..662
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            660..1853
                     /locus_tag="PW004_002184"
     CDS             660..1853
                     /locus_tag="PW004_002184"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HDL2727138.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1983..3455
                     /locus_tag="PW004_002185"
     CDS             1983..3455
                     /locus_tag="PW004_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2727139.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3538..4326
                     /locus_tag="PW004_002186"
     CDS             3538..4326
                     /locus_tag="PW004_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HDL2727140.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(4393..5061)
                     /locus_tag="PW004_002187"
     CDS             complement(4393..5061)
                     /locus_tag="PW004_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HDL2727141.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            5176..6888
                     /locus_tag="PW004_002188"
     CDS             5176..6888
                     /locus_tag="PW004_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2727142.1"
                     /translation="MKLILRHAKKYKVSVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6891..8663
                     /locus_tag="PW004_002189"
     CDS             6891..8663
                     /locus_tag="PW004_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2727143.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            complement(8745..>9447)
                     /locus_tag="PW004_002190"
     CDS             complement(8745..>9447)
                     /locus_tag="PW004_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2727144.1"
                     /translation="IFKFANRRYGLKYNPVNGVSLARKPKTNEEIKKEKDGYLSKEEV
                     QMIASKQKSSHQQSRYSLITKFLFLTGLRYGELISLTESDYDGNKITVSGTYDYELKI
                     KTTTKNTGSYRTIELSSNAKEIIDQLIEENKLIKNNKDKYIFISKNGNPISIQAYNQS
                     LRAVSKELNLDKKVSSHMLRHSHISLLTELGIPLKAIMDRVGHEDSKTTLKIYTHTTK
                     NMQNQLVEKLGKIEI"
BASE COUNT         2837 a         1582 c         2045 g         2983 t
ORIGIN      
        1 tggttccgtg gtgtaggggt taacatgcct gcctgtcacg caggagatcg cgggttcgat
       61 tcccgtcgga accgtttggc tcggtagctc agttggtaga gcaatggatt gaagctccat
      121 gtgtcggcag ttcgattctg tcccgcgcca ttatgcgggt gtagtttagt ggtaaaacca
      181 cagccttcca agctgttgtc gcgagttcga ttctcgtcac ccgcttttag tttttatggg
      241 cctatagctc agctggttag agcgcacgcc tgataagcgt gaggtcgatg gttcgagtcc
      301 atttaggccc atttatttta ttggcccgtt ggtcaagcgg ttaagacacc gccctttcac
      361 ggcggtaaca cgggttcgaa tcccgtacgg gtcatggaag atactttttg agtatctttt
      421 tctttttatc tgaaagaatg attttaaaaa gcttagcaga gttatcttta tggatgattg
      481 tgctaagctt ttttggacag ttctaatgta aaatattttt atatttttta agcaaaaaaa
      541 tgaaaaaata cgagcagaaa actgttttat ctgctatact tggttcgttg caagttcccg
      601 aaaggatccg caggacggag atgccttgta accgaattta actaggggga atcattataa
      661 tggtagaaag acacttattt acatcagaat ctgtttctga aggacatcca gataaaattg
      721 ctgaccaaat cagtgatgca atcttggatg caattttaaa acaagatcca acagcacgag
      781 tagcgtgtga aacgtctgta acaactggtc ttgttttagt atttggagaa atatctacga
      841 cggcatatgt agatattcaa aaagtcgtac gggaaacaat aaaagaaatt ggttatacac
      901 gtgcaaaatt cggatttgat ggagatacag cagctgtatt ggttgcgatt gatgaacaat
      961 ctcctgatat tgctcaagga gttgatgaag cacttgagat ccgggatgaa gataaaaaag
     1021 acgtattaga tgagattggt gctggtgacc aaggtttaat gtttgggttt gctgtagatg
     1081 aaacaccaga attgatgcct ttaccaatcg ctttgagtca tcgtctggta cgacgcttgg
     1141 cagatttgcg taaatcaaat gaattaacgt atttacgtcc agatgcaaaa tctcaagtaa
     1201 cggttgaata tgatgatcaa ggacaaccgg aacgcgtaga tacaatcgtt atttcaacac
     1261 agcatgatga tgcagtagat aatgaaacaa ttcgtcatga tgtcatcgaa aaagtagtga
     1321 aggaagttat tccagctgaa ttattagatg atcaaacgaa atattatatc aatccgactg
     1381 gccgatttgt cattggtggt cctcaagggg atgccggatt aacaggaaga aaaatcattg
     1441 ttgatacgta tggcggttat gctcgtcatg gtggcggtgc tttttctggt aaagatgcga
     1501 caaaagttga ccgttctgcc agctatgctg cacgttatat tgctaaaaac attgttgcag
     1561 cagggcttgc tcgcaaagca gaagtacagc tagcttatgc gattggtgtt gctcaacctg
     1621 tttcgatctc aatcaatact ttcggtactg gaacggttcc ggaagaagaa ctgattgctg
     1681 cagtgagaga aaacttcgat cttcggccag caggaatcat cgagatgctt gatttgcgtc
     1741 gtccgattta caaacaaaca gctgcttacg gtcattttgg tcgtacagat gtagatttgc
     1801 cttgggaaca tacggacaaa gtagatgcgt tgaaagcaag tttagcaaaa taagttatat
     1861 tcagctggaa cgatcgccaa gtggtggttg ttccagcttt ttttatggca gttaacctct
     1921 tgaaaaagat agtaattatt tatctatttt tagatcctgt cataatgaaa gggaggaata
     1981 agatgaaaaa agaaacaaat gttactttgg tcacagtgag tattttcatt gcaacgttca
     2041 tgacggctat tgaaggtaca atcgtaacaa ccgctatgcc aacgattgtc ggttcattac
     2101 atggaatgga aatcatgaac tgggtatttt cgatttattt gctgacaaac gctatgctta
     2161 ctccgattta cggaaaactt gcggataaag tgggacggaa accaatcttt atttttggga
     2221 cattattgtt tattatcggt tcgttatttt gcggtttatc tcaaagcatg attgggttga
     2281 ttgctgctcg tgcgattcaa gggatgggtg ctggggcaat gatgccagta gctttgacga
     2341 tcatcggcga tctttattct attgaaaagc gagcaaaagt tttaggtttg aatagttcag
     2401 cctgggggat tgccagtgtt tttggaccgc ttgcgggagg attcatcgtt gaaacgatca
     2461 gctggcactg gatctttttt atcaatgtgc cgatcggaat tctattaatt attttgattt
     2521 ggctatattt aaatgaacca aaaatcatac atgagtctaa accaatggat atttttggta
     2581 gttttagttt gatggccatg cttgtctcat tgctgttagg gttccaatta ttaggagacg
     2641 aaggaatgag tccgcttgtt tctgctttgc tgctaggtag tatcgtaatg ttaggtattt
     2701 ttgtttttgc cgaaaaaaga gctaaagatc ctgtcatcat gatgcattta ttcaaaaatc
     2761 cactatttgt tgtggtcaac ttggctgcag cattggctag tggttttttg atgggaatcg
     2821 atgtttatat ccctatgtgg atgcaaggtg tattagggct gaatgcagca attggtgggc
     2881 tggtattagc accattatca ctagtctgga tggtcggttc tttccttgca agcaagtgga
     2941 tgactcaaat gaaaactcat tgggtcttga gaattggact actaatcgca gctattgggg
     3001 gcgcttggtt gtatcttatg cctttcagaa gtaactattt atggtttttt gctatatcaa
     3061 cggtcatcgg tataggctta ggtgtgacca tgactacctc gactgtcagt gcacaaaata
     3121 gtgtatctcg tgaccagctt ggtgtagcaa cttcttttaa tacgttagtt cgaacaattg
     3181 gtcaaacagt catggtttct atttttggtt tattgataaa tggggtaact aaacgagaac
     3241 ttgcagcagc aaatttaaca aatgatgcag atatcatgaa taaactggtg aatccacaaa
     3301 cggctaaata tattgatgcg gatattttgt taccattaag aaaaattctt tatgatgggt
     3361 tacattccgt ttatctagtt ggcttgttac tcgtgatcat tgctttggtc ttaacgcttt
     3421 tcgttcatga aaaaagtggt aaaataaaac agtagtttcg gttaagatat gtacgtagcc
     3481 gaatcattca aatttaaagt tattcaaaaa taacttctta ctaaggaagg cgaaaatatg
     3541 gcatttattc aagcaaatat ttattcgaat gtgttagaaa tggaagtcaa tctaaatgtg
     3601 atactaccgc aaacaacaaa aaaggtgatt ggcaccagtt cagagcaaga gacaacggat
     3661 atacctgttt tatatttact tcatggaatg ggtggcaatc atagtgtatg ggaaagacgt
     3721 acgtcgattg aacgttatgt agcaaactac ggattagctg tcatcatgcc atctactgat
     3781 cttggctggt acacagatac agtttatgac atgaagtatt ggacattcat tgcagaagag
     3841 ctacctgaga tttgtcatga attattcccg caattaacaa gaaaaagaga aaaaacatat
     3901 gccgctggat tatcgatggg cggatatggc gcgttaaaac taggccttgc aaaatcagaa
     3961 aatttcgcag cagtaatctc tctttcaggc gctgtttctg taggaaatcg aatggatgat
     4021 cttttgatgg ttcgtaaagc atctttttgg gaagggatct ttggaccatt ggatcaaatc
     4081 gaaggttcaa aaaatgatcc gcaatactta ttgaaagaac tagtaaaaag cggaaaacca
     4141 gttccaagat tttatatggc ttgtggggaa tctgattttc tttatcaggc aaatcaggaa
     4201 atggtatctg ctatgcagga acaaggcttg gcggtcacct ttgaacaagg acctggcgat
     4261 cataactgga tcttctggga ccaatggatt caacgtgcat tggcgtggct aatggaagaa
     4321 aaataaaaaa agagtcttaa aaagatagct cctatagaga tcacaaacat agtgatctct
     4381 ataggagctt ttttactgct cgttttctct tcttttattt tgttggcata agccttcaat
     4441 ataggtttgt gcactcgtta gaaagagtgc tttttcattc aattctgcat gggactgcat
     4501 actttcttta aatccctcta attgattttc aactacataa ttacgcataa atagaactgt
     4561 ttctttcaaa aattcatttc cgtccataat tttttgtcga agctgctgtt catgctgctg
     4621 atccattaac tgtccaatca ataaattgtg gagagaggca atggcctgat gactttgggt
     4681 gactgaaaat ccttcacgaa caagtaattg gatcatttgc tccaaatgtt ctaatcgact
     4741 cggaaaagcg ggcacagtat tgatttctaa ttctgcccca caagggaaac gtgtataaag
     4801 gtcatagtaa ttttccatat actgcagcaa ctgttcattc caaggcaaag ttggatctgg
     4861 aagcgctaat tcttcttcca ttgtttcagc cattttttgc aagagctgtt gtttgttttt
     4921 gaaataccaa tataaagcag gtgcttgaac atgaattctg tttgcgatgt ttcgcatgga
     4981 aagctgcgaa agagcttgtg tttcagccag gatatcaaag gcggcctgaa tgatgattgc
     5041 ttgagaaagc tttatttcca ttttatcaac ctcgtgtatt gcattatctt tttaaacatg
     5101 gtaaattgtt agtcggtcta attttacagt gttaaattag tcctgtcaat ttaaacaaag
     5161 gggtgtaatc aacgaatgaa attaatctta cgacatgcga aaaaatataa agtatctgtt
     5221 tttatttcct tgctgtccgt agcagtcatg gttgcagcag cattatggca acctaagtta
     5281 ctacagcaag tattggaagc tattattaca gaagataatg atgaaatgaa aacaattggt
     5341 atttacctga tcagtctggc tttgctagga ttagccgcag gtgtaacgaa cacgattttc
     5401 tctgcaaaag tagctcaagg cgtaagtgca gatatccgag aagaagcatt tcgaaagatc
     5461 cagacatttt catttggaaa tatcgaacag ttctctgctg gaaatcttgt tgttcgtcta
     5521 acaaatgata tcacgcaaat ccaaaattta gtgatgattt ctttgcaatc actttttcga
     5581 attccattct tatttattgg tgccttcatt ttagctatga tcacgatgcc gcaattatgg
     5641 tggatcatcg tagcgcttat cgttacggta tttctgatta cggcactttc atttacaagg
     5701 atgggcaagc acttcatgat cattcaaaaa ctgattgatc gtgtgaatag cattgcaaaa
     5761 gaaaacttac tagggattcg tgtagtgaaa tcttttgtac aagaagaaaa ccaattgaag
     5821 tcattcagca aagtaagtga ggacttaaca aaacacaaca tcatagtcgg tacattattt
     5881 tctgtgatga tcccgtcctt catgctggct gccaatttgg ctgtggttgg ggcgatcttt
     5941 tttgtgagcg atcttgttaa agatgatcct acgttaattg gtggaattgc ttcattcatg
     6001 aactacttga tgcagattat gatggcaatc attattggcg gaatgatgat gatgatgact
     6061 tcaagagcag ccgtatcgct gaagcggatt gcggaaattt tagatgctga accagatctt
     6121 acttatttag acgttccaga acaagagttg acgggttctg tcacttttga ccatgtttct
     6181 tttcgttatc ctggtgagga tacagatacg cttaaagata tcagcttttc tatcaaacct
     6241 ggagaaatgg ttggtatcgt cggggcgact ggtgccggga agtcaacact agcacaattg
     6301 atccctcgtc tatttgatcc gacagaaggc aaagttgaag taggtggtgt agatcttcgt
     6361 caagtaaatg aaaagagttt aagacaaacg gtttcctttg ttttgcaaaa agcaatttta
     6421 ttctcaggaa cgattgctca aaacttgcgc caagggaaaa agaatgcaac agaaaaagag
     6481 atggaacatg cttcttctat tgctcaagca aaagaattca tcgaaaagtt atctgatcgt
     6541 tacgaagcac caatagaaga acgtagcagt aacttttctg gtggtcaaaa gcaacgttta
     6601 tccattactc gtggcgttat cggtaatcca aagatcttga tcctagatga cagtacgagt
     6661 gcactggatg ctcgctctga aaagctggtc cgagaagcac tcgatcgaga tttgaaggga
     6721 acaacgacga ttgtcatcgc acaaaaaatt gcttctgttg tcaaagcaga ccgcatctta
     6781 gtattggatg aaggcagatt agtcggcgaa ggaacccatg aggaattggt agctggaaac
     6841 cgtgtctatc aggaaatctt tgaaactcaa aaaggaacgg aggagtaagt atgactgatc
     6901 taataaaagc aagtaaattt ttctatcact atttgaagcg gtacaaactc tcctttttct
     6961 ttatctttat cacagtgatc attgctacct atctacaagt aaaggcacct caatacatcg
     7021 gggaagcttt tcaagaatta gctaattacg taggaggctt gatgcaaggc gtagacgata
     7081 aaagcaaatt tgtttctgta atatggaaac tcttgctgtt ttatgtacta gctagtgcag
     7141 ccaactttat ctatagtatt ttgttcacac aagtagtagg gaagtcaacg aaccgaatgc
     7201 gtatcggtct attcaacaaa ttggaaaaat tgacgatccg tttctttgat tctcatcaag
     7261 acggtgaaat tttaagtcgt tttaccagtg acttagataa tattcaaaat agtttgaacc
     7321 aagctttgct tcaagtcatc acgaatgctg ttttacttgt cgggatcttg atcatgatgt
     7381 tccgccaaaa tgtagagtta gcttgggcta caattgcttc cactccagta gccgttttga
     7441 tagctgtctt gattatccgc aaagcacgga aatatgtgga tcttcagcaa gatgaagtgg
     7501 ggaaactaaa tggttatatg gatgaaaaaa tcagcggaca acgtgtcatc atcacaaatg
     7561 gcttacaaga agaaacgatt gaaggattct tagagcataa tcaaagtgtt cgcgaagcaa
     7621 ccttcaaagg acaagcttat tctggtctac tatttccgat gatgcaagga atgtcactgg
     7681 tcaatacagc gatagttatc tttttcggcg gctggttagc attaaatggt gatttggaac
     7741 gttcggtggc attaggctta gttgtgacat tcgtccagta ttcacaacaa tattaccaac
     7801 cactgatgca gatttcatct ggatacagca tgatccaatt agcgattaca ggggcaagac
     7861 gattgaatga aatatttgat gaaaaagatg aagtcaaacc agaaaacggt aaagcatttg
     7921 atggtgttga aaaaggtctt gcgttgcagc acgtagattt tggttattct ccagaaaaac
     7981 tagtcttgaa agatgtctcg atcaatgtgg acaaaggaga aatggttgct cttgttggtc
     8041 caacaggatc ggggaaaacg acgatcatga atctgctaaa ccgtttttac gatgtcaatg
     8101 atggttcagt agtcatcgat ggaatagata tccgagatta tgatcttgat gcattacgta
     8161 gacatgtagg aatcgttttg caagaatctg tattgttctc cggaacgatc aaagacaata
     8221 tcacattcgg caaaccggac gcttcagatg aagaagtcat caatgcagca aaacaagcga
     8281 atattcatga cttcatcatg acgttagaaa aaggttatga tacagagata tccgaagaaa
     8341 ataacttatt cagtacaggt caaaagcagt tgatcagtat tgctcgaacg atcatcacaa
     8401 atccatcttt attgattttg gatgaagcaa caagtaatgt agacacggta acagaagcaa
     8461 ggatccaaaa agcaatggat gaagcaatca aaggaagaac cagctttgtg attgctcacc
     8521 ggctgaaaac aatcttgaat gcagatagaa tcgttgtctt gcgtgatggg gaagtcattg
     8581 aagaaggcag tcatcatgag ttattagcag aaaatggatt ttatgcggaa ctttatcata
     8641 atcaatttgt atttgaaagt taaattaaaa gaacctcgtt ataacggggt tctttttata
     8701 tattttatgt tgtttgtttt tataaggggc agaaaagggg caatttaaat ttctattttt
     8761 cctagttttt ctactagttg attttgcata tttttagttg tatgagtata aatctttaat
     8821 gttgtttttg aatcttcatg tcctacacga tccataatgg cttttaatgg gattcctaat
     8881 tctgtaagta gagaaatgtg ggaatgcctt aacatgtgag aagaaacttt tttatctagg
     8941 tttaattctt tggatactgc tcttaaagat tgattatatg cttgaataga tatcgggtta
     9001 ccatttttag agatgaaaat gtatttatct ttattattct taattaattt gttttcttca
     9061 attaattgat ctatgatttc tttagcatta cttgataatt caatggttct ataagaacct
     9121 gtatttttag ttgttgtttt aatttttaac tcgtaatcat aagtaccaga aaccgtaatt
     9181 ttattaccat cataatcaga ttcagtcaaa gatatcaatt caccataacg tagtccagtt
     9241 aaaaacaaaa atttagtaat taaagaataa cgtgactgtt gatgtgaact tttttgttta
     9301 ctagcaatca tttgaacttc ttcttttgat aaataaccat ctttttcttt ttttatttct
     9361 tcgtttgtct taggttttct tgctaaagaa accccattta cgggattata ttttaatcca
     9421 tatcgacggt tggcgaattt aaaaatc
//
LOCUS       DANKKG010000110         9200 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000110 DANKKG010000000
VERSION     DANKKG010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.110"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            49..243
                     /locus_tag="PW004_002191"
     CDS             49..243
                     /locus_tag="PW004_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727145.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            512..811
                     /locus_tag="PW004_002192"
     CDS             512..811
                     /locus_tag="PW004_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727146.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(908..1342)
                     /locus_tag="PW004_002193"
     CDS             complement(908..1342)
                     /locus_tag="PW004_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2727147.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1574..2284)
                     /locus_tag="PW004_002194"
     CDS             complement(1574..2284)
                     /locus_tag="PW004_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDL2727148.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2274..3134)
                     /locus_tag="PW004_002195"
     CDS             complement(2274..3134)
                     /locus_tag="PW004_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HDL2727149.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3139..3777)
                     /locus_tag="PW004_002196"
     CDS             complement(3139..3777)
                     /locus_tag="PW004_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HDL2727150.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3792..4091)
                     /locus_tag="PW004_002197"
     CDS             complement(3792..4091)
                     /locus_tag="PW004_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2727151.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4134..5618)
                     /locus_tag="PW004_002198"
     CDS             complement(4134..5618)
                     /locus_tag="PW004_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDL2727152.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5638..6099)
                     /locus_tag="PW004_002199"
     CDS             complement(5638..6099)
                     /locus_tag="PW004_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2727153.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6117..7184)
                     /gene="ulaG"
                     /locus_tag="PW004_002200"
     CDS             complement(6117..7184)
                     /gene="ulaG"
                     /locus_tag="PW004_002200"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HDL2727154.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7402..8172
                     /locus_tag="PW004_002201"
     CDS             7402..8172
                     /locus_tag="PW004_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL2727155.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8445..9152)
                     /locus_tag="PW004_002202"
     CDS             complement(8445..9152)
                     /locus_tag="PW004_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDL2727156.1"
                     /translation="MSPGINILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSV
                     SKYQESYKILQTIDQEKVSNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTE
                     TDIHHLQAAANVAMIYLSKGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYR
                     KIEDYDKAIQLCEKGIEYNKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIA
                     RFNGNDYAVKVIENDLK"
BASE COUNT         2853 a         1893 c         1464 g         2990 t
ORIGIN      
        1 acatatattt gaatattaac taaaaacagc aagaaagaag aggatcaaat gtattatgta
       61 gaagtaaaaa caaaaggcgt caaaaacaaa caatatgtga aaggtatcag taacgaatat
      121 cctttacttg gttcatggaa agaagctgct cctttttcca aaccatgtgc cataaaaata
      181 aagaacgagc tagaaaaaga actgacttgt ggtaaagctg ttgtagatat catcgaaaag
      241 taaatccaat gtatagaaag gaagatcaca catgaaaagc gacgtacaaa ttgctaagat
      301 tatagaatta gaagaagaag acgcagtgtt gacaattgaa aaaaaataaa tgtttgtata
      361 ccatatgatt atttcaatat gtatcctgga attggagatc tcgtagagct atatcaagat
      421 gaatcaaata tacttgtcat tcatcatgaa atatagagac ttataaaatt gtttattttt
      481 ataatgtatc agtatagaaa ggaaataaaa catggattat actgttgaat ttgacagtga
      541 actacttaag caatttccac aagaagaaca tttcatgtat aaagaagcaa tttcccacaa
      601 agtgacagag tatttggagc ataatttttt tcgtataaag ccagtgcgta ctcgttcggc
      661 aaaaggaata tacgagatga aagtcaaaat taataaaaaa aattatcgca tagcatttgc
      721 tttaaaaaaa aatcatgtgt tcatttttta tattagttcg aatttgcaaa aaattgtatt
      781 tgataaagag gtgagtaaaa aaataaaata gctgagcatt ggaggataga ttgagtgata
      841 ctaagtctat cctcttgttt ttctaaagaa ctaagctaga attttttctt ttgtacgtta
      901 ttatatgtta tttgtcatta ccaaaaagca ccttttttct tctagtatct atgacataag
      961 aaataccacc catttccttt acttgaacaa atcctactga ttcataaaat ttttttgttt
     1021 tctcagtatc atctgtcaat aattcgattt ggactggtcc ataactagtg atcttctcca
     1081 aagcttcatt caaaagcact ttgcctattc cttggcgctg atacctcgga tgaatgagga
     1141 tatcttgaat aaatgcgatc gtcgcaaaat cagtgatcca acgaacgatt ccgataatct
     1201 tcccttcccc attacgcttt atcagagatt cagattgttc aaatgctcta gctaaccttg
     1261 cagtgtcttt tgtgtaattc gtccaaccaa cgctcttata taattcagaa atctcatcga
     1321 tcataaggga atcaatattc atttttttct tcctttctta tacaattctc cctctaataa
     1381 tacagttctt tgactattta atctatcaca tataactctc ctgtaaagat tctctcgatt
     1441 ccttaaaatt cctctttcct tttagaatgc atactttttg ataaataaaa aggttgtgat
     1501 aaattttctt cacaacctct gtttctctct atcaagtgtt atcttttaat tttctaccct
     1561 taccctagca tctctattgc ccgtagtatg catactttcc atgtttgcga aaataatgtt
     1621 tatctagtaa atattgcggg atttcacata tattggggtt gatcagctcg ttttctttgg
     1681 ccatcagaca aatttcatct aagatcaaac tgttttctac tgctttcata ggtgaatccc
     1741 cccatgtgaa cggtccatgt ccatagacaa gtactccagg gacttcgttt ggatctaatt
     1801 ttctttcctt aaacgtctcg acaatcacat tccctgtatg aacttcgtaa gcttctctta
     1861 cttcttcttt cgttaattgg cgtgtacaag gaaccttacc ataaaaagca tcagcatgag
     1921 tcgtgccata agctggcaaa tcgcgtcctg cttgcgccca catgacggag tgcgtggaat
     1981 gagtatgagt aatagcagtt atatcttcaa aagtttgata caaaacaaca tgtgtgggga
     2041 gatctgaaga tggttttaag ctatcttctt ctattatatt tcccgacaaa tctgttacca
     2101 ccatttgatc tgcttgcatg cattcatatc tgacaccgct tggcttaatg acgatgactc
     2161 ctagctcacg gtccacttca ctcacattgc cccaagtcaa tttgactaat ccgtatcgcg
     2221 gcaatgccag atttgcttca aggacacgtt gcttcattgc tgcaattttt tcttcacaag
     2281 acatcatagc cagcctcctt caatttagaa cttaagtagt cttttgcctg ctggatttct
     2341 cttcgaaagt cttggctttt ttcactccac atttcaatca gaaatgttcc atcataatct
     2401 agacgtttca aatgtcttaa taatccaaga aaatcaacac aaccgctacc aaaaggaaca
     2461 tcacgaaact ggcctgaata ttcttttgtt acagggtaag tatcttttaa gtggatagca
     2521 gtgatataat cgatcccttt ctcaagttct cgagccggat tattttccgg ccatgcggac
     2581 aaattgccta ggtctggata tacttgtaga aaaggtgaat gaatctgttc tttgatttct
     2641 aaaaattttg aaatggagtt cataaatgga tcatccatga tttccacgga aagaatgatg
     2701 ccatatttag aagcttcctt tacgctttct tttattcctt cgataaagtc ttctctggaa
     2761 ttcattgatt tctcttcata ataaacatcg tatccagcta tttggattac tttgacgctt
     2821 agattatgcg ccaaatggat tgcttttttc attagttgtt tcgctgcttt tttcttttct
     2881 ggatcagcag aaccaaacgg aaatcgccga tgcgcactta aacaaatcga atggatacgt
     2941 acaccggagc tgaacatctc ttctctcagc tcagctatct tttcttcaga ccaatctaat
     3001 cgtgctaacc gctcatccgt ctcatcaatc gacatctcga taaaatcgaa gtccatttct
     3061 tttacaaggg aaaaacgttc tttccatgaa atatctttgg gtaacgcttt ttcataaatc
     3121 ccaatttgag ccatttcctt acccccagat acgaatgatc tcatctttga agttttgtgc
     3181 ggccttcatc ggatcatttg ctgctgtaat accacgacca gtgataaagg tatacacatc
     3241 gatcccttca aataagtgta atgtgtgtgg atttaatcca ccagttacag aaacattaaa
     3301 gcctagttca atcagttttt tgatcttcga taaatctttt tcaccccaag tttcaccaga
     3361 taacagcgca tcacggcttt gatggtagat tacctgccgg ataccgatgt tatgccaatc
     3421 catggcttgt tcaaaagtcc aattgccgta gagttctact tgcaattcac ctatttcttt
     3481 ttgtgctgcc tccatagttg gaatcgttgc acaacaaatg actgtcatcc agtctgctcc
     3541 tgcttttgca acattactcg ccactgttcc tccagcgtct gcacatttcg tatcagcgac
     3601 tagtttttta tctgggaaca tactgcgaat acagcgaatc gctttttgtc cttcctgtaa
     3661 gcaaagaatt gtgcccactt caataatatc tactacatca ccaactttca taacatctcc
     3721 taatgcatgt tctaagtcat tgtgatctaa cgcaatttgc aaattcggcc tgctcatctt
     3781 agttccttcc tttacttgtt tttttggtac cactttgttt cagcaaattt ttcactgatt
     3841 tctttgactg acatcacatt tttgataccg ataactgtaa ctcctttttt ctctgcctga
     3901 gtaaacatat tcaaaaaatt gactggcgta aaaactacat cgtattggcc agctgaactt
     3961 tttccctctg aaatggcaca atgatggatt ttggtaattg gtacgtttag ttctttcatt
     4021 gctttttcca cacttctcat catcatcaag cttgttcctg atccatttgc acacgatact
     4081 aaaattctca tcttaatccc tccattatga gtgttgtcta atcagatttt cttttatact
     4141 ctctgctttt ctgcatatgt gtcatagtct tctacaatca aaaaataacc atcaggattt
     4201 ttccgatatt gtaactgcgg aatagccaat aaaccaatga caactacggc aattccagca
     4261 taacctagga ctttcatgat tactgtaaac aatggccaag ctgttgccca gtcgaacatc
     4321 ccgatataac caccgtatgt tgacatccca atccatgtag cgaataatgc tgaaccgcct
     4381 acttgtatga ttcctgaaag aaacgggaac aggcaagcgg ctttgaatcc acctcggtta
     4441 ttggcataaa ctgctataac ggcattatca aagaacaacg gaatgaaacc ggctatgacg
     4501 atcgttggtg atttcaataa aatcagtaaa ccaatcatca aaaactgtcc caaagcacca
     4561 aacaagaacc caatcgttac cgcatttggc gaaccaaagc caaaagtagc agctacgtca
     4621 attcctggaa cagctcctgg taaaatcgtg tttgagattc cttggaaaga ttgagttagt
     4681 tcatcgacga atgtccgaac acctaattgc aagatcgcca gatataccgc aaaatttaaa
     4741 gaagtcgtca taatgtagaa taagaaactc tgcccttctt gcatgaattg cgcttgaatc
     4801 aaataatctt tgcctagtac aatcagaatg atcccaaaga aaaatagcat gagtaacgaa
     4861 gtagcaacca tattctcatt gaaaatcgac aagaatcctg gtaatttgat atcttcaatt
     4921 tttttgtctt gcttgattga tctatttttc ttttcatccc gctttttcat ccactcagct
     4981 aattttgcaa atataaagat tccgaacatt tgctgatgtg ccactgcaaa tccggcacct
     5041 tctgtcaatt cctgcgtgat tcctactgtt aagttagaag ctactgtcca ataacaacct
     5101 aaaatcagtc ccataaaaat caatgcttgt atcctaccta gatcaggaaa gcaaaacagt
     5161 aaaatccaaa aagcggtagc tgcttgttga acctgaacgt ttccggtagt gaaaacggaa
     5221 cgcagtttcg tatatttctg aaaacggact aataagatat ttaaaacaaa tgcaatcagt
     5281 aataaaatca tcacatctcc aaaggtacgg ccaaatgtct ccattaatcc agcatccaca
     5341 gcattttgtc caaaataagg atctgtaacc atggcatcca aattgaaccg ttcctttagt
     5401 ccaaccaaga ttggccgaaa attattgacc aaaccaccag agccaacagt caaaataaag
     5461 taaccgaccg ttgcctttaa gaatccagcg atgcattcat gaagcgggcg tttcagcaaa
     5521 atatagccta ataacacgat aaaaccaatc aggtatgctg gctgcgtcaa gatatttgtt
     5581 gcaaaatacg tccaaatgct taacaaaaat tcccccatct tttttctcct ctatcaatta
     5641 attatattct gccatcacaa cttggtaatc atcgatggag ttcgtagaga tgagacgctc
     5701 aaccaatcca tctgtcatta ataattcgga taatttttgg atattttcta gatgttcttc
     5761 tggatctttt gctgcgagtg taaaaaacaa actagccgtt ttctcctcat tctccccttc
     5821 actttcaaaa cggattggct gtttcaactt ggtaaatgaa atagctgttc caaaaacacc
     5881 atcacttttt tctgaagaat gcggcattgc tacttgaggt gcgatcacaa tataaggacc
     5941 gtatttttct acacaatgga tgatctcctc cacatacgtt tcatcaataa tttccttatc
     6001 gatcaataca agacagcttt cggcgatagc ttcacgccaa tccgtgattt ctttatctgg
     6061 gtaatgaatc aattgatggt caaaaaagta tttcagcaag aaactcccct tctttcttat
     6121 aaaaacgacg gaaacggtgt atcattttca atcgagaaga catcttcaaa tcctcgataa
     6181 aaattaaact caagatcatc tttgtttgtc ggatagatga atttcccgcc tacttgccag
     6241 atataaggtt taaatccata cttcaggcga tcctttttga atttccaaat ttcagtgatc
     6301 tcttttggat cagccataaa attagaccaa atatcgtagt ggacaggaat cactactttt
     6361 gcattcaatg actccgccat gcgcagcata tcaacagaag taactttgtc tgttatccct
     6421 cgtgggtttt caccataggc ccctaaacag acatcgattt tgtgttcatt accatgtttg
     6481 gcaaataaat tggagtaatg tgaatctcct gcatgataaa gattccctcc tgatgtttcg
     6541 aataaatagt ttactgcaat ttgatccatc tcctgaggca tttttccttt taatacctct
     6601 tctggatctt cacaagtgac caacgctgtc cgatcaaacg cttctaaagc tactacattg
     6661 atgtctttga ttttgactgc atcacctggc tttactgtaa tcgttttttc ttcaggaatt
     6721 ccccattttt gccacgtgcg aaccacttct tttggcccaa tgaatgttgc ttcaggacaa
     6781 ttttgatgaa ctgctgcggc tgtatggata tctagatgat cggaatggat atgtgtaacg
     6841 attaaagcgt ctacattttt tatcgcaaaa ggatcgatta cgaaaggctg agtacgtaaa
     6901 ttaggctgca tattttgaac gccactcata cgcatcatct gatggccttt tttcatcatg
     6961 ccattcccat gactacgttt tcccgtccca caccatagat cacataaaat attcgttcct
     7021 tcatgacttt tcagccaaat tcccgtacag cccagccacc acatagcaaa agttccagga
     7081 tccactactt ctgtttcgat ttcttcattc aagtatgtgc cccactcagg aaatgtttct
     7141 actaaccact tttcttttgt tacttcattg acagttttac tcatttgctt gctcccccca
     7201 tatcgttgtt ccaacttaca agtaaatatt aatgtgtatc agcatccatg aaaacccttt
     7261 tatatgaccg tttgtttttt atatgatttt ctttcagtaa tcaatgattg tttcagtata
     7321 ataaagacag atatgaatgt ttaaatgaac aactagtcaa ataaatccat ggatcaagat
     7381 gtccctcagg aggcaaaagt catgaaaaac tcacttgtca atattgaaaa acgtcaaaaa
     7441 gaaattttgg aactgcttca gcagactgaa caactatcaa ctttcgaatt agctgaatcc
     7501 cttgatgtat ctattagtac gatacgaaga gatttaaatt tattagaaga aaaaaacgag
     7561 atcatacgca attacggtta ttgttcttat aattacaaaa accagactga ttttgatcaa
     7621 tctggtcctg aaaggattaa acaagcgatt gccagagaag ctagtaaata cattaacgat
     7681 tatgatacgc tgtttatcaa ttctagctcg actgcgttga aaacactgga ttttcttcaa
     7741 tctaaccatt tgacagtcat tacaaacaac gtgaaaatca attactctcc gcacaaagat
     7801 aattgtagtt atattttaac tggtggagag atgcgctttc caaaagaagc actagtgggc
     7861 gacatcgcat taaatacgat ttcttcagtc aatgcagaca tttgtatcat tggctgtagc
     7921 ggagtcgatg tagtacatgg ggtcacaaca aagatattaa atgaatcaac gatcaacgaa
     7981 atgatggtca acaagacaat caaatgtaag attttagtcg ctgatcatcg aaaaatcgga
     8041 ctgacatcaa agttcaagat tgctgatatt tcagtctttg attatttgat cactgataaa
     8101 ttttgttctg aaaaagtatt agaggagatc agaaatacgg ggataaaaac cattcaagta
     8161 gattcatttt aagaaccagg acgtaaatat gtaaatagga ttatcaaaaa aatgaataac
     8221 aaatttatcg ataaatgaaa aacacgctac gctcaaattg ataaaggaat cccaagatgt
     8281 atccatccaa tcgataccat cataatctat tgcttctttt ataaccgtta tatgttgcct
     8341 aaataggaat caaacaatat cgaaaaaagg aggtatgaca aaagtcacac ctcctttttt
     8401 cgaataaacg gtattcaaat cagaacactg atttcacaac cactttattt caggtcgttt
     8461 tcaatcacct ttaccgcgta atcatttccg ttgaaacgcg ctattttttt tgcgtccata
     8521 tatctttcta aataatcatc ttctcctaat tgcttatggc aagaagcaat ttcataaagc
     8581 aagtattcca atgcataagt cgatttatgt ttcttattat actcaatacc cttctcacat
     8641 aattggattg ctttatcata atcctcgatt ttacggtaat aggtggcgat atcataatat
     8701 acaatgcaga ccatcagatt atcaaaatca gtctcactca atattttaag tgtacgctcc
     8761 acataaactt tcgcaaaatc taattctcct tttgacaagt agatcattgc cacgtttgca
     8821 gctgcttgta agtgatggat atctgtttcg gtcgtttgtt ccaacacttg attaaaataa
     8881 aataatgctg catctggttt tttcgcataa agatgatcaa gcagtccttg ataaaataaa
     8941 atttgattta aaagatcaga attacttact ttttcctgat cgatcgtttg taaaatttta
     9001 taagattctt ggtattttga tacagaattc aatgattgaa tctcatttat ttttttcctg
     9061 attttgtctt cttctgtttc tactaatata tcggagatct cgacattcag ctttttgcaa
     9121 agcgcactta agatattgat gcctggcgac atattcttgt tttcgatatt gctgacagtt
     9181 gcttgttcac aaatgccttc
//
LOCUS       DANKKG010000111         9030 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000111 DANKKG010000000
VERSION     DANKKG010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9030
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.111"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..1383
                     /locus_tag="PW004_002203"
     CDS             <1..1383
                     /locus_tag="PW004_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2727157.1"
                     /translation="LAALVIGSAIGGGVFGIMTDISGSAGGPAIFSWVLVGTAMLMLS
                     LSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLGNVAFATLLMSALGY
                     FFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTFCKLVPLFLFIVVAI
                     STFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVGIEGASVVGSRAKKQ
                     SDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMGYVFEMMVGKWGAVV
                     INGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNGAPTYAIVLTTLCTN
                     VFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKEWVQFLVGLLASAFQ
                     VWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDKILLIGVTMVGVIAM
                     VLFATGMLHV"
     gene            complement(1564..2337)
                     /locus_tag="PW004_002204"
     CDS             complement(1564..2337)
                     /locus_tag="PW004_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL2727158.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(2334..3239)
                     /locus_tag="PW004_002205"
     CDS             complement(2334..3239)
                     /locus_tag="PW004_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2727159.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(3393..3713)
                     /locus_tag="PW004_002206"
     CDS             complement(3393..3713)
                     /locus_tag="PW004_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HDL2727160.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            4187..4873
                     /locus_tag="PW004_002207"
     CDS             4187..4873
                     /locus_tag="PW004_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HDL2727161.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            4875..5819
                     /locus_tag="PW004_002208"
     CDS             4875..5819
                     /locus_tag="PW004_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HDL2727162.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            6018..6662
                     /gene="pcp"
                     /locus_tag="PW004_002209"
     CDS             6018..6662
                     /gene="pcp"
                     /locus_tag="PW004_002209"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HDL2727163.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            6741..8018
                     /locus_tag="PW004_002210"
     CDS             6741..8018
                     /locus_tag="PW004_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727164.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            8276..>9030
                     /locus_tag="PW004_002211"
     CDS             8276..>9030
                     /locus_tag="PW004_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2727165.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEESTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQ"
BASE COUNT         2840 a         1645 c         1862 g         2683 t
ORIGIN      
        1 cttgctgcct tagtaattgg ttcagcgatt ggaggtggcg tatttggtat tatgacggac
       61 atctccggat cggctggcgg cccagctatt ttcagttggg tactcgtagg aacagcaatg
      121 ttgatgctct ccttgtcgat caataatatc aataaaaaac gtccagattt aaaaggcggg
      181 atctttagtt acgcagaaga aggttttggc cgtttcgctg gttttatcag cggttggggt
      241 tactggctca catgctggtt aggcaacgta gcttttgcaa cattgttgat gagcgcatta
      301 ggatatttct tcccagtatt tgaaggtggg caaaatctgg catcgatcgt gatgagtaca
      361 gtctttttat ggggatatgc atggctagtg aatcggggag tcgaaaatgc tagtttcgtc
      421 aatgctatcg tcacattctg taaattagtg ccattatttt tatttattgt agtagctatc
      481 agcacgtttt cgatccaaca attcattgat aacttttcta gcgacattat attgaatgcg
      541 aatggacagc cgatcccgtt ttccagtcaa gtattgagtt gcatcatggt gatgctttgg
      601 gtatttgttg gtattgaagg agcctctgtc gtaggttcac gagcaaaaaa acaatcagat
      661 gttgggaaag caacgattct agggatattc ggcttgatta ttatttacgt cttggtttcc
      721 atgcttccat atgggacaat gacgatgaat cagctgcaaa cgatcaaggc gcagcctgct
      781 atgggctatg tgtttgaaat gatggttggt aaatggggag cagtcgtgat caacggcggt
      841 ttgatcattt ctttagttgg tgtttggctc tcatggacga tcttaccgat tgaaacaatg
      901 ggaaacatgg ctgaagacgg acttttgcct aaagcatggg gaaaactcaa caaaaatggg
      961 gcaccaactt atgctatcgt attgacgacg ttatgtacga atgtcttttt actttcatta
     1021 ttattcactg acagtgctta taactttgct tacacacttg gtacagcagc tattttcttc
     1081 acgtggcttt tcttaggatt gtaccaaatg aaactttctt atcagcggaa agaatgggtc
     1141 caatttttag tagggctttt agcaagtgcc tttcaagtat gggctatgct agtcgtagcc
     1201 tttaatgaag taatgcttgt tcttgttctt ttcttgccag gcatttattt ttacctcaaa
     1261 gcacgtaagg agcaaaacga gcctgtacaa catatccact tagataagat tttacttata
     1321 ggtgtgacga tggtcggagt gattgcaatg gttttatttg ccactgggat gcttcacgta
     1381 tagaagtata aaagaagtat aaagagaggt tgtgagcctg tcgtttattc aaaaacatac
     1441 taggaaagga gctgtgacac aaatgttgtc acagctcctt tttcagaata aacggtgttt
     1501 aagagacagc caatcggcaa taaggctgaa cgtctctttc acagcctctt ttttgatgag
     1561 tcatcataca ttttttcgct taaatacgag atatcctaat gctaagaaca aaacgatgta
     1621 agctaaatta ccgaatagca tttgattagt agaaagatga gtcaattgct tcatgatctc
     1681 ttcgtttcca atttggtttt gcagattcaa catactgatc ggattccatt tgatccactc
     1741 ccatttttgg atgagtgcaa taaggatacc agatatgatt gacgaagcaa aataaaagac
     1801 gattcctgct gagatggatg cgccggaact attgataaag caagcaatca tcaacactaa
     1861 acttaaaatc agccataaac cgatataact acccaaagta taagtgaaca agctttggat
     1921 cagtgtttga ccagtagaaa ccttctccgt gaaggaaatc gaagggaaaa gaatcagttt
     1981 catcaacaca gtgacaataa tagctaaaag gtataaataa acagaataaa taactaatgt
     2041 gatccatttg cttactaata tttctcctcg agaatacttt cgataaagga gatttttcaa
     2101 tgtaccatat tgtgcttcca tcgaaatgat cgtgcttgca gctgcaatca tgataaagac
     2161 aatccaagat gtggcactaa aaagctgagc agtcatttca actggatcaa tgatcgtcgt
     2221 gtattttttg gctagaagtg ctgttcctat gagcaaaaca actaatagaa cagataaaac
     2281 agcagttgac tttttcttga tcaatttaaa taattcttgt tttacaagca atgtcatttt
     2341 tcttcttccc cctttactga atccagaatt tccaatagag aagattccaa atcattcgtc
     2401 agatgctgga cgtctgtcac atggatattt tgtgcagaca atagctggat cacggttcct
     2461 aataattctt cgttttcttt taaaagcttg attttatcag gatcttccaa agtatatcca
     2521 gcttctgtca agatcgttcg agcagctaga tcatttgttg tagagataac gatgaaatct
     2581 tgcgctgttt ggttcaattc agccattgta cattgtttga tgatctttcc ttgattgatc
     2641 aggatcagct catcgaccat tttttcaagc tcgctgagta agtgactgga gatcaagaaa
     2701 gaggtgccct gagtagccaa gtcggtaatg atttttcgca catctcttgt tgcttgtgga
     2761 tccaaaccgt tcatcggctc atctaaaata acgatagacg gatggttcag taaggcaaga
     2821 gcaattccca atttttgttt cattcccaac gagtaggatt tcgctttttt gtgaatgtac
     2881 ttttccatct tgagctgatt cacgatagtg tcgatagcag atacatcgtt ggtttcagag
     2941 aataacttca gatgatcata gcctgttaag aatggataga tacccgggta ttcgatcaat
     3001 gctcctactt gttccaaccc tttatgacta gagggagaaa tctcattttc accaatacag
     3061 attgttccat ctgagtaatg gatcagtcct aaaatagctt tcatgatcgt tgtttttcca
     3121 gcgccattag gacctactaa tccaacgatt ttgccagggg ataagctaaa actgacatcg
     3181 ctgagggctt gtttgtgacc aaagttcttg gataattctt ggaccgaaag acttttcaaa
     3241 aatattcact ccttttctat agatagtaca agcatacacc tgatgagtgg agaagtcgct
     3301 aggtcattgg acttatttct tattttttca taaaaaaatt gaatattaaa taaaaaggct
     3361 tattttttct gatcagtact tattccaagt gtctagtggc cgctagtaat cttgatgcca
     3421 atgatactga tgagtagcat ccccacaaag aaccaagtgg aaagattcag cttatcatga
     3481 aacaagacca caccaattat gaccgaacca actgcgccaa tccctgtcca gataggataa
     3541 gagagactca tcggcaaact tttgattgcc tttgacaaaa agtaaaaact agcaatcatg
     3601 ccaatcactg tgtaaaaaga ataattgatt ttagaaaaac catcactcca tttcatcatc
     3661 gttgaccaga atacttccaa aattcctgct atgattaatt ccatccatgc catacttaca
     3721 ttcctcctta actataaaaa aatacacaaa aagaggaaaa tcatcccttc aaagacctaa
     3781 tggaatgatc atcctcttta tcctacccgg tggcagattg tatatctttt tctgtagtat
     3841 agcacaccta tccagatatc ggcaatctca tactttttag taaagtgtac caagcactga
     3901 cggatatatc gttagcggaa aagaagatga atgctttgca atttcataga aagaagttgt
     3961 tatttattaa tcatttaaaa aaaacagtaa ctagacctta tttctatata ttatcgggca
     4021 ataaattaaa aaaagcttta ccttacaata agtaagataa tttttgtgat aaaaaaataa
     4081 cgaaaaatat ttaaaaatag tgtctatttc gtgagaaaac aactatattt tttatgtgtt
     4141 ttgctttata attaaaaaaa tattgtcatt ttatggagga aaatatatgg aaaattatct
     4201 tagcctttta ggcgtcttga ttgttattct cgggtttgca tttaaattag actcgatatt
     4261 gatcgtaatg gttgctttag ttgttacagc tttgactggc ggtcttggca ttcaaggaat
     4321 gttagaactt ttaggtacaa gttttgtaaa caatcgcggg atggcgattt ttatcatcat
     4381 tatgttggca acaggaacgc ttgaaagaaa tggtctgaaa gaatcagcgg cttccttgat
     4441 caagcgattc aagaaagtat ctgctggtat gattgtagat atttatggga tctttcgaat
     4501 gatctttgcg gcttttaacg tgagttttgg tggagttgct ggatttgttc gtccaatcat
     4561 tttaccgatg gcattgggga ccatcgaatc aaaagggtta gaagtccatc ctaagcatga
     4621 agaagaacta aaaggtatgg cttcagctat ggaaaatgtc tgctggttct ttggccaagt
     4681 actattcatc ggaggagcag gggcgttgct agtccaatca acactgaaag atctaggata
     4741 tgacgtaaca ttgggacagc ttgctttagt ggaagttcca gtggctatca cggctttagt
     4801 tgtggcaagt gtctacttct atcttaaaga aaagaaatta gcgaagaaat attatccagg
     4861 aaaggggcaa taagatggac ttttttacaa atcctgatat tttattaggc gataaattac
     4921 tggagatcct ttatatcgtg atgggactcg tattgattta cactggagta agaaatttaa
     4981 cagataaaac caatcctcat cgttatgggt cagccttttt ctggacggtc ttaggggtag
     5041 ttattgcagg aggaagatgg cttccggcaa tcgtgaacgg tggcttgatc tttgcgatga
     5101 ccataccagc aattgctaag aaagtcagca aaggagaaag ccggcttcca tcaaaagaat
     5161 atatggaaaa aatgtcagac aaactaggaa tgaaaatttt tattccagct ttgagtatcg
     5221 gtgtctttgc gatcttgttt gctctattta ccaatttagg tgctttagtc ggcgtgggcg
     5281 tcggtgtttt tgcagccatg ctaatcttga tgttcttttc tcgagataat cgaccaagta
     5341 catttttaga tgatgcagca gatatgctgg gcacagtagg gccgttaagc atgttgccaa
     5401 tgcttttagc ttctctaggt gcggtattta caagcgctgg agttgggaca gtgatttcca
     5461 atgcagtagg tacgatcgtt ccagaaggaa atgtgaacat cggcatcatc atttacgctc
     5521 ttggcatggt gatatttacg gtaatcatgg ggaatgcatt tgcggccatc accgtgatga
     5581 cggttgggat cggtgcacca ttcgtattta gtcatggagc gaatccagcg ttaataggaa
     5641 tggttgcatt gacttgcggt ttttgtggga ccctgctgac accgatggct gctaacttca
     5701 atatcgtgcc tgtagcgatg ctggagatga aagacaaata cggagtgatc aaaaaccagt
     5761 tgtttattgc gttatttatg ctagttttcc aaattgctta tatgattcta tttaaataat
     5821 agaagagagg ggttgtgaca gaataattgt cgcaacctct tctaacattg tagtggaaca
     5881 ctattttttt ataagaatat attaagtttg atctgttatg ttatttaaac tattgtttcc
     5941 tttatttacg ctgttcaagg aaaatggtat gataaaagat aaccatatgt taaatcaatc
     6001 ggacggagga acaacagatg aaagtactag taacaggttt tgatccattt ggtggggaca
     6061 aagtgaatcc agcctatgaa gcagtaaaaa aaatgccaga tgaaatcagc ggtgctgaaa
     6121 tcatcaaagt ggaagtaccg accgtattcg aaaaaagcag tcaagtagtc aaagaagcca
     6181 tccaacagca ccaaccagat gtcgtgatct gtgtcggaca agcaggtggg cggtcagcag
     6241 tctcgttcga acgtgtcgca atcaatctag cagaagcaag aatccctgat aatgaaggaa
     6301 atcagccatt cgatacggct ttagaagaaa acggtccagc cgcttacttt acctcattgc
     6361 caatcaaagc aatgacaaaa aatgtacaag atcatggtct accagcgtac atctcttata
     6421 cagcaggaac atttgtctgc aatgatatca tgtatcgttt gcttcatttg atcgaaacac
     6481 aattcccggc aattcgaggt gggttcatcc acgtgccatt ctcacccgat caagtgatcg
     6541 atcgtccagt gggtacgcct tctatgtctt tagaagacat tgcttcttct ttgacttatg
     6601 cagttgaagc agcagtagaa aataaagaag atatttccgg aaatgccgga acaactcact
     6661 aatttagcaa gaaacactgg ctggttgcta acgttatgtt agtagccagc tttttgtaat
     6721 tagaaaggaa agaaacagcc gtggaagaat ggcaatccgt ctttgaagaa tggtttccaa
     6781 aagaaatcag caaaagctat cccatcaaaa tttctaaaca atacacaagc agtcaacgtt
     6841 gggagatcta tgcaaaatta acgaaaaaac agcgggaact agtcgataag caccgccgat
     6901 atctaatcag ctctcgtttt atggaagaac attatttagc tgcaacagat tgggtgtttt
     6961 ctgacttcaa gatcaatccg ttttttcgaa caaaacgaag ccagcaaaaa ttgtattgtg
     7021 agtgtggtag agaattaaaa gtgcaataca tcgttaaatc gccaaaaacc ggaaagatcc
     7081 taaaactagg catcaaccac tttgccgatc acctgcatgt ctctccgaca gtggcagctt
     7141 cgatccatca gggaatgaca aaagttgatc tggccttaga cgaattatta tggcttaagc
     7201 agaagaatat cgattttcct gaaggactat ggcaaaaata ctgttttgtc ttgtaccaaa
     7261 atcgtcggat gaaacagcca tatttaccgg atataaaatt agctcagcgg ctcgcagaat
     7321 ttcgccaagt agaaatgccg atttacatag cggattatca agcattggaa aatgaaatca
     7381 agaaaataag tgaacacatc aatgggcagt caaaaaagcg acagataaaa aaagaactat
     7441 ttgacgactt tgcagaagaa ttagtaaagg atgtagaaga attcttgatc aactatcgcg
     7501 catttttacg taaagattgg caatccatcg tatatgaaga agtgccagta catcctaatg
     7561 cttattttga aacatttatt tccgtgttga gaaagacaaa acgtcaacga acaccagaag
     7621 tcactgctca gatggaatat tttgcgaaaa accaacgatt catccaacca aaaatctatc
     7681 tatttatttg gaagcaatat tgtcgctatg gttttacaga agggttcttc gacagcattc
     7741 ctcggatagt cagaaatggt tttttaaaag tattgcgtaa agaacgagaa gctatacaat
     7801 ctgctgataa gaaagaccgt acagtctcaa aagaaaaatg gcaactagta gtgaaagata
     7861 tccaatctgg aaatgtgcaa gagacaatcg acaaatggaa aggaaaacat tatcgtttta
     7921 ctgaagctca gaaacaagcg ttggaatatt atcagaagtt agaagaatca ctgcgtttca
     7981 atgatgaggc aagaaagtat ctgaaggaat tactataaaa aacatactaa gattagaggg
     8041 gaacatcatt gacgaatgat gttctcctct attttctatt tttagattat agtgaagagt
     8101 gaaagaaagt tcgaacaaag ccttgaggac tacgagtccc tatcaaaaac cgaacttctt
     8161 tactgtcatt ttagaatcag gtttacgtat gttggggcca tgagcccgtg tataggaaat
     8221 tggaatcgaa atgaagcagt aaagttcttt caaaacttaa aaaggaggaa aacaaatgcc
     8281 ttacattctt gcattagatg tttcaatggg acacagctac agtgtcctat acaaagacga
     8341 cacctgctta tttgaagacg agattgaaca cactcaaaga ggatttcatg cattactcga
     8401 agagatccag cacttgcctg agcgtccatt gattgttttt gaagcaacgg ggatttattc
     8461 cagaccagtc gaaaagttct ttcaagatca tcacttttct tactgtttgt taaacccgtt
     8521 agaggcgaaa aaacagatgg aagaaagcac cttacgcagt tggaaaacag ataaatcgga
     8581 tgcccaccgg ctcgcacaga ctcatcttaa aaatcaacgt cagccaaaag aagtacaaag
     8641 caatttttac ttagagatgc gcgatttagc ccgtttttat caagaaattg agaagaaaat
     8701 cactcgtctg cgcatggatt tacacaactg ccttcaattg acttttccag agcttgaaca
     8761 gttcttttca aacaggctga ccccttacgc attgacattg atccgtcttt ttccccatcc
     8821 agattttgta ttggcatcta ctcggacaaa aataaaaaat aaactgatca acgaaacacg
     8881 gaaaaaaatc tccgcgaatc gtgccgagca aaaggcagat caaatcatcc actatgcaca
     8941 gtgtgcctat ccagctgttg aaaaagacag tatccactgt caaaaaacga tctattacgc
     9001 cgaacttctc caagatttac tcgaacaaaa
//
LOCUS       DANKKG010000112         8909 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000112 DANKKG010000000
VERSION     DANKKG010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8909
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.112"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            13..237
                     /gene="nrdH"
                     /locus_tag="PW004_002212"
     CDS             13..237
                     /gene="nrdH"
                     /locus_tag="PW004_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HDL2727166.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            396..2555
                     /gene="nrdE"
                     /locus_tag="PW004_002213"
     CDS             396..2555
                     /gene="nrdE"
                     /locus_tag="PW004_002213"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HDL2727167.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            2605..3570
                     /gene="nrdF"
                     /locus_tag="PW004_002214"
     CDS             2605..3570
                     /gene="nrdF"
                     /locus_tag="PW004_002214"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HDL2727168.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(3659..4798)
                     /locus_tag="PW004_002215"
     CDS             complement(3659..4798)
                     /locus_tag="PW004_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL2727169.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(5107..5820)
                     /locus_tag="PW004_002216"
     CDS             complement(5107..5820)
                     /locus_tag="PW004_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HDL2727170.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(6074..6775)
                     /gene="nagB"
                     /locus_tag="PW004_002217"
     CDS             complement(6074..6775)
                     /gene="nagB"
                     /locus_tag="PW004_002217"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDL2727171.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            7009..8541
                     /locus_tag="PW004_002218"
     CDS             7009..8541
                     /locus_tag="PW004_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDL2727172.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(8732..>8909)
                     /locus_tag="PW004_002219"
     CDS             complement(8732..>8909)
                     /locus_tag="PW004_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2727173.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         2973 a         1742 c         1570 g         2624 t
ORIGIN      
        1 gaggcactca ccatgaacgt aaaaatcttt tctaaaaata attgtatgca atgtaagatg
       61 gtcaaacggt ttttaacaga aaatcaaatt gcttttgaag aagtcaatat cgatgaacag
      121 cctgaagcag tagactggtt gaaagcacaa ggattccaaa gtgttcctgt cattacttct
      181 gatgcagaaa cagttatcgg attccgtcct gatcagttgc gtaaacttgc cagctaacat
      241 gactaaaaga atcaaaacgt gatacatgga tttgaagggc ttgagacagc accagttttt
      301 gtcgcaaact tccaaatcct tttttaaagc ttaaaaacaa tgcaatgatt tgcaatcaag
      361 catcatcaaa tacgaaatga aggttgggcg tacgtatgag tcttaaaaat ttacaagatg
      421 tcagttattt caagctgaat aatgaaatca atcggccagt caatggtcaa atccctttga
      481 ataaggataa agaagcttta gttgcctttt ttgaggaaaa tgtcaaacca aatacgatga
      541 cttttccatc tattatggaa aaaatcaatt acttgatcga tcaagaatat atagaagcag
      601 aattcattca attgtattca ccatcattta ttgaaaagct atatcaattt ttggacgagc
      661 aacatttttc tttcaaatct tttatggctg cttacaaatt ttattctcaa tatgcgttga
      721 aaaccaacga cggttccgct tatctggaaa cttatgaaga tcgagtagcc ttcaatgcgc
      781 tttactttgc gaacggggat gaagaattag cacttgctct tgcagacgaa atgatccatc
      841 aacgatatca gccagctaca ccttctttct tgaatgctgg acgtaaacgt cggggcgaat
      901 tggtgtcttg tttcttgact caagtcactg acgatatgaa ctctatcggg cgctcaatca
      961 actctgcttt gcaattgtcc cgtattggtg gcggcgtagg gattacttta tcaaatcttc
     1021 gtgaagccgg cgctcctatc aaaggatatg agggtgctgc aagtggtgtc gttccagtca
     1081 tgaaattatt cgaagatagt ttcagctatt ctaatcaatt aggtcagcgt caaggagctg
     1141 gcgtggttta tttgaacgtt ttccatccag acattattgc tttcctttca gcaaaaaaag
     1201 aaaatgcaga tgaaaagatt cgcgtaaaaa ctctttctct tggcgtgatc gtacctgata
     1261 aattttatga attgacacgt aaaaacgaag atatgtattt atttagtcca tatagtgtcg
     1321 aaaaagaata tggtgtccca ttctcttatg taaatattac agctgagtat gatcatctag
     1381 ttgcaaatcc aaacattcgc aaacaaaaaa tcaaagcgcg tgatcttgaa aatgaaatct
     1441 caaaattaca acaagaatct ggctatcctt acgtaatcaa tattgataca gcgaaccgcg
     1501 agaatccaat cgatggcaag atcatcatga gtaatttatg ttcagagatt ttacaagttc
     1561 aaacgccttc tgtgattaac ggcaaacagg aatatgaagt attaggaaca gatatctcat
     1621 gtaacttagg ctcgacaaat atcgtgaatt tgatgcaaag ccctaacttt ggtcaatctg
     1681 tgcgtacgat gactcgtgca ttgacattta ttacagatgc atctgatatc gatgtcgtac
     1741 caccgatcca aaacggaaat aagctgaatc atacgattgg attaggtgcc atgggcttac
     1801 acacttatct tgcaaaagaa caaatggaat atggctcaga ggattcatta gactttacaa
     1861 atatttactt catgctgttg aattattgga cacttgttga aagcaacaat atcgctcgtg
     1921 aaagaaaaca agttttccat aattttgaaa aatcagctta tgctgacggc acttatttcg
     1981 aaaaatacgt aactggagaa ttccagccaa aatcagaaaa agtcaaagca ttgtttgaag
     2041 gcatttttat cccaactgct gaagattgga atgctttgaa acaagcagta atgaaagatg
     2101 gactgtatca tcaaaatcgc ttggcagtag caccaaatgg ctcaatttca tatatcaacg
     2161 atacgagtgc aagtattcat cctatcactc gcatgatcga agaacgccaa gagaagaaaa
     2221 tcggtaaaat ttattaccca gcgccttatc tatccaatga gacgattccg tattacactt
     2281 ctgcttatga tatggacatg cgtaaagtaa tcgatgttta tgcaacggca caacaacacg
     2341 tcgatcaagg catgagtttg actttattca tgcgttctga aatcccagaa ggcatttatg
     2401 aatggaagac aacaacgaag caaacgacac gtgatttgaa tattttaaga cattacgctt
     2461 tccacaaagg aatcaaatcc atttattacg tacgtacctt tactgatgat gcagaagaaa
     2521 tcggaagcaa tcaatgtgaa agctgcgtga tctaatatca tagatagatt cgcttattca
     2581 tattttaatt aaaggagcat tctcatgtca gaaacatatt atgaagcaat aaactggaat
     2641 gaaattgaag atatcattga taaatccact tgggagaagc tgacagagca attttggcta
     2701 gatacacgta tcccactttc aaatgaccta gatgattggc gtacattgtc tcaattggaa
     2761 aaagatacag taggacatgt atttggcggt ttgacactgc tagatactgt ccaatctgaa
     2821 agtggcatgg atcagttgcg taaagatgtc cgcactcccc atgaagaagc cgttttgaac
     2881 aatattcaat tcatggaatc cgttcatgcc aaaagttatt cttctatttt tagtacactg
     2941 aatacgaaaa aagaaatcga agaaattttt gactggacaa atacgaatcc ttatctgcaa
     3001 aaaaaagctg agcgtatcaa tgaaatctac aaacacggta caccgctaga aaagaaaatt
     3061 gccagcgtct ttttagaaac attcttgttc tattcaggtt tttatactcc tctttattat
     3121 ttaggaaaca acaaattggc taacgtagca gagatcatca aactaatcat ccgagatgaa
     3181 tctgtacatg gaacctacat cggttacaaa ttccaattag gattcaacga gttacctgaa
     3241 gcagaacaag aaacgttaaa agactggatg tacaatcttc tttatgagct ttacgaaaac
     3301 gaagaacgtt atacagaaga attatacgat ccaatcggct ggacagaaga agtcaagaca
     3361 ttcctacgct ataatgccaa caaagcattg atgaacctag ggatggatcc actctttcca
     3421 gatactgcca atgatgtcaa cccaatcgta atgaacggga tttcaacagg cacaagcaac
     3481 catgacttct tctcacaagt cggaaacggc tatttgttag gtcatgtaga agcgatgaaa
     3541 gacgacgatt atttgattgg attggaataa ggatttattt taattcaaaa atttttcgaa
     3601 aaataaaaag agtttagcgt acagatattt taaatgtaca ctgaactctt ttttatgctt
     3661 attcacttgt ttcttccagc acttttggac tgtctaactt ttccgaaaca tgttccattc
     3721 ttctcaataa atagattttc cggatattta atattatcgt cttaatcact gcatagacag
     3781 gtatgcaaat cagcatgcct aatattcctg cgacacttcc tgctccaatc aataccaaga
     3841 tgatcgtcaa aggatggata ttcattgatt tcccaaataa caacggcttg atcaaatttc
     3901 catctaattg ttggatcact aaaatcgaca acgccatata caaagcttga aatggagaga
     3961 taaacaaacc gacaatgatt gctggtaccg ctccaataaa agggccgaca tacgggataa
     4021 tattcgtgat tccgcaaatg accgcaagca acaagctata aggctgatcg aatattctca
     4081 ttgccaaaaa gctcattaca ccaataatca aagcatctaa gatcgtactg ctgatatagg
     4141 cagataatgt ttcgtttaat tcttttaccg tttggcgtaa ctctttacga atcgctttcg
     4201 gaaagaagtg agacaacgca tcaataaatt tgtgcccatc cttgaacata aatagcaaaa
     4261 taaatggaac ggtaaataac aaaataaaaa attgcatcat aaatgaaatg atttttgaga
     4321 cacttgcaga taaactgact aatatgatat tgaacaaatt actaagagat aaatttgctg
     4381 atgctaattg ttcttccaaa ttgaaatttt taaactcttc ccgctgcgcc aattcattta
     4441 accatttgct tgtttcatct gcatacattg gcaagctctg ggtcaattga attgtctgtt
     4501 ccattaactg aggcactaca ttcatgacag ttaaaacaac taaaatgatt acgaccaaaa
     4561 acgtcaataa aaaaccgaac actctcggga tccgatgctt ttcgagaaaa acaacgattg
     4621 gatcgaacat ataatagata aaaccagcaa caagcaatgg taaaaaaatt gccgaaagca
     4681 ttttcaatat tggactaaaa atattcggca tttgtaaaat aaagtacacg ccgatgatcg
     4741 tgagtactag ctccactgtc cagaaaaata attttgattg tttaaaacgt gattgcatac
     4801 atctcctcca aggtcctttg atacttctat tctatcattt aacaaagagt tacgcgctga
     4861 gattttcatt tgcttaaaaa aaacaaaaaa aataaacata ttttaatgag tagacgagta
     4921 cttaaacaaa aacacggttt ggagtagtga caaaaattat actgcagtcc ctttttccaa
     4981 ataaacggtg ttcaaaagct tgcatcttcg taataagccc agccaaacga aaaatataag
     5041 gaactatttt tatttggctg ggcttattac ttgatgtagg acatctattt tacaacctca
     5101 gatcatttat tgcgcaatac tgactaaatg caactttgtg atatttttgt gtacggacaa
     5161 ctcctcgatc acatcggcat aagtgagccc ttttggaaga tcaatcgtat agatattttt
     5221 gtacactcgg tagtcatcaa cgaattttac gtcaaaattc acatcttcga ttacgatatt
     5281 tttctcttca aaataatcgg taagaaaatc tttggtttct tttctatgga taaattggat
     5341 ttctaacttt ttagtcgtag gtacatggat cacgcgtttc actactgtga gtgcaatagc
     5401 aatcccgata aaactgatga tcgcaatgga atagtaaccc atgccgatgg cgatccctaa
     5461 tccagcaatt gcccataaag aagcggcagt cgtaagtccc gtcaccgatt gtttcgtgac
     5521 gatgatcgtt cccgcgccta agaaaccgac accactcacc acttgagcaa tcagccttgc
     5581 ttcatctgaa cgaatcgttc cagcaagctg tggatactct aaagcatctc ttaatgcacc
     5641 actagcgatc tcaacttgga tcaatgcaat gatacatgct cccatacaaa ctaaaatatg
     5701 cgtacgcata cctgctggac gatttttata ttgtcgttca aaaccgattg ctccgccaaa
     5761 tagcatagcg atccctaaac gtaaaataat ctctggaatt gtcaatacca tatcattcat
     5821 ctaaacaccc tccagtccaa tatatcatgt ctgaaaggag cagacaaaaa aagtgcaatt
     5881 tttacctctt acgaaaaaag gactgtgaca aaatttttgt catagtcctt tttatgaata
     5941 aacggtgttc aatcagctga ccctcggtat tactcaacaa acggcaaaat atggggagct
     6001 atttttcctt tgtcttctct aatactcagg tcaaaacgct gatttcacaa cctctttagc
     6061 ctactattga aaattacaac ttacttgctg cttcttcatc gatgatcaca acaacattgt
     6121 cgtgattttg caatacactt gctggcaaat gattcgttac aggtccttca atcatccctt
     6181 tgattgcatc agctttgctt tcgccaaatg ccatcaagac gatttcttta cttttcataa
     6241 ttgaaccgat tcccattgaa acagcttttg ttggtacatc ttctgcttta tcgaagaatc
     6301 gtttgtttgc attgattgtt gattctgtta agtcaactac tgaagtcgtt ccgtcaaatg
     6361 aagcaccagg ttcattgaaa ccgatatgtc cattttgccc gattcctaag atttggatat
     6421 cgattggatg tgcatcgatg atttgatcat actttttgca tgctgcttcc agatcttctg
     6481 cttttccatc tggtacaaac gtttctttga atggtttttt atcaaataat tggacatcca
     6541 tgaaatgacg gtagctttgt tcatctgaac cgcctagtcc aacgtactca tctaagttta
     6601 cagaagtcat ttcactgaaa tccaaatcgc ttgaagtcat ttctttatat aaagtaactg
     6661 gtgtgcttcc tgttgccaat cctaatactt ttgcaccttt gttcatacct tctttgatga
     6721 tttcaaatgc tttttttccg ccttcttcag cgttttttac acgaatgatt tccatagtga
     6781 ttgctccttt atattttggt atagcccaat tatagcattc tttttttctt tttacaataa
     6841 ttagtataga ccaaattgca tttttttcat tttcttgaca ataaatagga atccgtgtta
     6901 ggtttcataa gatggttcaa tcaaacaatc catgttgcat gaaaaccaag caagtgattc
     6961 actgtggacg ctttatgtct gcagtttttt tatgaaaaag gagttattat gagtatactg
     7021 acattaaaca acattaccca gcaatttggc gacaaaatcc tctatgaagg tgtcgatctg
     7081 caattaaatg ctggcgaaca tcttgggctg attggacaaa acggagccgg taaaagtaca
     7141 ttgattaaaa tcatcactgg tgagatctta ccagatgacg gacagatcca ttggcaaaaa
     7201 aatattcaca aaggttacct cgatcaatat gtggaagtcg atgaaacatt aacaatcgaa
     7261 gaatttctaa aaactgcgta cgcaaaagaa tacgaaaagg aagaaaaaat tgcttttctt
     7321 taccaaacat atagcgagac cttcgatgat tccttgctgg aaaaagctgg gaaactccaa
     7381 acggaattag accaaggagt gttttatcaa atcgacacct tagttgctga gatgagcagc
     7441 gggttgggga tcgatgtctt aggtttagat tcactgttga aaaatctaag tggtggacaa
     7501 cgttcaaagg tgattttagc aaaactactt ttagaaaacc cagatgttct gatcttagat
     7561 gaaccaacca atcacttaga tgatcagcat atccaatggc tgacgcagtt tcttcaggat
     7621 tttgacggtg cgtatatcgt gatttcccat gaccaagaat ttcttgatca gatcaccacc
     7681 catatcgcag acatcgagtt tggaaagatt actaagtata ctggccatct aaaacaagca
     7741 ttgaaacaaa aggaacaaaa tcgcgaaagc tatttgcgcc aatatcatgc tcaacaaaaa
     7801 caaatcgaaa agaccgaagc ttatattcga aaatacaaag ccggttcccg atcaacgatg
     7861 gcaaaaagcc gacaaaaaca attagataaa atcgaacggc tgacgcctcc agcttcagca
     7921 agtaaagctg tctttgattt tccattctcg cctattgtca cgacattagc agtggaaaca
     7981 actgatttag tcatcggata tgatcgaccg ctacttgcac ccatcaattt gaccatccgc
     8041 tttggtgaaa atattgccat tcgaggcttc aacgggattg gaaaatctac tttattaaaa
     8101 acactgatcg gtgagattga aaaaataagt ggcgatttcc acttcccaga taacacaaaa
     8161 atcaattact tttcacaaga tctgtactgg gaaaatccat tagaaacacc actacaatat
     8221 ttaagcagtc aatttccaaa agcgactatc aaagaattac gtcgccaact agctaaagca
     8281 gggttagtta atcaattagc aagtgaacct ctcactacat taagtggtgg ggaacaaact
     8341 aaggtgaaac ttgcacagtt gacaatgaac caaggtaatt tactgattct cgatgaaccg
     8401 acgaaccata tcgatcaaga aacgaaagaa agcttacaag acgggataca gaaatttcca
     8461 ggaacagtaa tcatcgtctc tcatgaacaa gaattttatc aagatcttgt gcaacgagtc
     8521 attgagattg aaccaaaata aacgtttgat tgaatagttg aaaaaaagga gctgtgacaa
     8581 aagttttgtc acaactcctt cttctgaata aatggtggcc aattagctga ccttcggtat
     8641 tagctcaaaa aacggcaaaa tatgaggagc tatttttatt tggctattct tattacttga
     8701 tgcagaacag cttttcacaa cctcttttcg tctatttttt attttcgaga tttctctttc
     8761 ctttaaaaac aactgtttga aagaagaacg cacatccttg aaaaacaagc ccgctaaccg
     8821 ttacacctaa ccagccatac tgctgccaca ttgtcgtccc tagcaatgaa ccagccgcac
     8881 ctccaataaa gtacataaac ataaataca
//
LOCUS       DANKKG010000113         8837 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000113 DANKKG010000000
VERSION     DANKKG010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8837
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.113"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(128..748)
                     /locus_tag="PW004_002220"
     CDS             complement(128..748)
                     /locus_tag="PW004_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HDL2727174.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            complement(824..1261)
                     /locus_tag="PW004_002221"
     CDS             complement(824..1261)
                     /locus_tag="PW004_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HDL2727175.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            1424..2254
                     /locus_tag="PW004_002222"
     CDS             1424..2254
                     /locus_tag="PW004_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HDL2727176.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            2238..3605
                     /locus_tag="PW004_002223"
     CDS             2238..3605
                     /locus_tag="PW004_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL2727177.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            3624..4433
                     /gene="yidA"
                     /locus_tag="PW004_002224"
     CDS             3624..4433
                     /gene="yidA"
                     /locus_tag="PW004_002224"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HDL2727178.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(4476..4919)
                     /locus_tag="PW004_002225"
     CDS             complement(4476..4919)
                     /locus_tag="PW004_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HDL2727179.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            5053..5424
                     /locus_tag="PW004_002226"
     CDS             5053..5424
                     /locus_tag="PW004_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL2727180.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            complement(5593..6375)
                     /locus_tag="PW004_002227"
     CDS             complement(5593..6375)
                     /locus_tag="PW004_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HDL2727181.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(6372..7343)
                     /locus_tag="PW004_002228"
     CDS             complement(6372..7343)
                     /locus_tag="PW004_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HDL2727182.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            7435..8829
                     /locus_tag="PW004_002229"
     CDS             7435..8829
                     /locus_tag="PW004_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDL2727183.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRTQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
BASE COUNT         2738 a         1795 c         1506 g         2798 t
ORIGIN      
        1 tttatttttt atgtatttac ttgggatttt tatatacaaa aaagaaggtg tgacaaaaat
       61 cgtgtcacac ctccttattc tgataggctg atcgtctctg tcacaacctc tcgtaagaaa
      121 ataatgttta ttctttttca gagtcagaaa attcttcctc ttcataatct tcatcatcat
      181 cgtcttcgtc tatgatactt aaatcttctt cgatatttcc tggcaagtct tcttcatcat
      241 ctgtcgggtc atctgctccg atttcttgca agtcagaatt ataagcagcg atttcttcat
      301 cttcatcatc atcgtcatca aacagatcat cgtcatcttc ttctgtcaga tctgtatctt
      361 ctgggtcatc gtcattgtaa tcgatcgcgt cttcatcatc attgataaag gcgttgactt
      421 tcttgcgttt acggcgacgt ggagtatctt catcctcttc gtccattcca tggtttactt
      481 cttcatcgat cgaatcgata gcataccatg aacgcaagcc ccaacgatta tcccctaatg
      541 agataaagct gccgtcaata ttcaagtcag tataaaattg tgcgagtgaa tcacgaattt
      601 cactgtctga tttcccaaga tagttttgga tctggttagc taaatcagaa aaatccatga
      661 catcgccacg ttgttctaag atcgcgtgtg ctacttcgat cattgataat tcgcttttgt
      721 ttgcaccttc aaatacacta atttccaatc aggacacgtc ctttcacagt ctactcctta
      781 tcatacaaaa tcagacacaa aaaatcaaac ttttctttta tatttacgct gtaaaatcca
      841 aggtcaaata atattcgccg attttttccg gtcccatgaa cagatcataa tcgattcgaa
      901 ccttccctgc tgtgggacgg tctttcaaac taacatgcag ttctttcgta aaagtagcaa
      961 tctggatcat accgtatggt gttcgatagg atgtttctaa ccgttcttta tagccaaatt
     1021 tcaaacgcat ccgcatttct cctgcacgga tcagctgtac atgaccatct ggaactaatt
     1081 tgatcgttac aggaatctca tcgcccgtct cctcctgaat ctctttataa cgtatgtaca
     1141 gcgtatcgcc aattgtcacc atttgccctt tcaaatcaaa ataaaaatct tgcacttccc
     1201 cttgctgttt tacttttgta cgcaatttga ttgaaatcgg tgttccttgt gataattcca
     1261 ttattcattc tcctttactt ctcatagcta cattgttcct agtttctttt gttttgtcaa
     1321 ccaacttgtc catttttaca gaaaaccttt tttccaaaac aactctttga ttttagagta
     1381 taatattgta gaaaatatga atttatacaa aggagccggt tgaatgaacc ctgcgattcg
     1441 actagatcag cacatttatc aaaaaaacga cttttcccct tttgcattaa cagatatatt
     1501 aacggaatat tctaaagaac accagcaaat ctttttacat ttctggcaat atgactgcac
     1561 agtcatctta ggtatgaagg atgttcgaac tccttttcta agtgacggga tccaaacact
     1621 gaaagatgca ggttacgctc cagtgatcag aaattcaggt ggattaggga tcgtatcaga
     1681 tgaaggaatt ttgaatattt ctttgatttt cccccaaagt gacacaaaaa aaataaccat
     1741 agatgacgct tacgagcaaa tgctcctatt gactaaatac gcttttccaa aggcaccgat
     1801 acaagcattc gaaatcaccc attcttattg tcctggaact tatgatttaa gcatccatgg
     1861 caaaaaattt gccggtatcg ctcagcgtcg gatcaaaaat gggatttctg ttatgatgta
     1921 cttaagtgtc aacggtaatc aggaaaaacg cgggcaagtc atgaagcgat tttatcaatc
     1981 tgctttaaaa gaagactttg gtacaaatgg ctatcctgca gtggatcctg ctacaatggc
     2041 aacacttgaa gaactgcttg accaacccct ttttgtaaaa gaagtcaaag aagcatttgc
     2101 aaaagctttt gaaaagctgt attctgcaag tgatttacag gattctaccg actggctaaa
     2161 aaaagaaatc cagctggagc aatggaacaa ccaaatccag agaatggaac aacgtaataa
     2221 cataaaggag ctacattatg acaacccctt ataaatatca aatgctgcct atggaaaaag
     2281 tttttcgtga tccagtccat aactatatcc atgtccagca tcaagtaatc ttagatttga
     2341 tcaattcgaa agaagtccaa cgtctacgac gcattaaaca attaggcact tcttctttca
     2401 cttttcacgg agcagaacat agccgtttta ctcattcatt aggcgtttat gagatttcaa
     2461 gacgaatctg cgatatcttt tctcgaaatt tttcaaaaga aaaaataggc aacggaggct
     2521 gggatgatca ggaacggctg gtgactttat gtgcggcact gttgcatgat gtcggtcatg
     2581 gtccttactc tcatacattt gaacatatct tccatacgaa tcatgaagcc atcactgtag
     2641 agatcatcac ttctccagaa acagaagtgt atcagattct taaccgagta gaagaaggtt
     2701 ttcctgaaaa ggtagctagt gtgatccaaa aaacttaccc caacccacaa gttgtccaaa
     2761 tgatttctag tcagatcgat gcagatcgaa tggactattt gcttcgggat gcttatttca
     2821 ctggaacaga atatggaaca tttgatctta cccgaatatt acgtgtgatt cgtccttatg
     2881 aaggcgggat cgctttttcc atgagtggca tgcatgcagt ggaagattat atcgttagcc
     2941 gatatcaaat gtatgtgcaa gtgtacttcc atcctgtttc ccgtggtatg gaagtaattt
     3001 tggagcatct gcttcatcgc gcacacgagt tgtatcaaga agatgccagc agttttgaac
     3061 tccattctca attacttctg ccatttcttg acggaagctt tactttagaa gaatacctca
     3121 aacttgatga tggggtcttg ggcacttact ttactcaatg gtctgaatct gctgatccta
     3181 ttttaaatga tttagctaaa cgttttttga atcgtaaacc actgaaatct gctacttttt
     3241 ctggcaaccg ggattcaaaa ttagtccaag aactcacttt gttagtagaa aaggtcggat
     3301 ataatcctgt ttattacact gctgtgaatt ctagctacga tctaccttat gatttttatc
     3361 gcccagaaca aggcagacat cgcacacaaa tcgagatttt gcgaaatgac ggaactttga
     3421 tagaattatc acaggttagt cagcttgttg ctgcattagc gggacaagaa caaggagatg
     3481 agcgattttt ctttccaaaa gaaatggttg atccgtcctt gcgtgatcat tatgatttat
     3541 ttgacgaaac ttatcaagaa tttgccagcc atatccgtaa tggcgcattg attgaaataa
     3601 attaaaatat attggaggaa attatgtcta tcaaactagt cgctatcgac atcgatggta
     3661 ctttattaaa tgagaaaaga gaagtaacaa aagaagtaaa agaagcgatt gccgctgctg
     3721 ttgcaaaagg tgtttcgatc gttttatgca ctggccgtcc cttaccaggt gtccaggaac
     3781 agttaaacga gttgaacctt tttcaagata atgattacgt tattacttat aacggggcac
     3841 tcgtacaaca aacgaaatca ggtaaaatca tcgcccgtca cggactgaca catgaagatt
     3901 tcctagagat cgaagtgatg gcacgccgtg tcggcagtca tctccattcg atcgatgaca
     3961 aagcgatcta cacgcctaat cgagatatca gcgcttatac gatccacgaa gcaagtttag
     4021 tcaagatgcc gctaaaatat cgaacacctg aagaaatgac accagacatg aacatcgtca
     4081 aaatgatgat gatcgacgaa ccagaaattt tagatgcagc aattgcgcgt ttaccgcaaa
     4141 ctttccgtga taaatacaca acggtcaaaa gtgcgcccta ttacttcgaa gtactaaata
     4201 aagaagcaag taaaggtgcg gcagttgcga atctcgcaca gcaccttggt attgatcaag
     4261 atgaaatcat ggctatcgga gataacgaga acgacttatc catgattgaa tatgctggtc
     4321 taggagtagc aatgggaaat gccgtttctc ttgtcaaaga agcagcaaat gtcatcactt
     4381 catcaaacga cgagcacggt gtagctgaag caatcaaaaa atatgtactg taataaataa
     4441 aaaaactgag agaaagctga tctactcgct ttctttcagt ttttattttt ttcgatccat
     4501 ctctcgactg cccatcggtg gctaccacgt ttcaatttca caagcgcttc ttgagggctc
     4561 acccaccaaa ttttattggt cttttcagtt ggttctccta tacgcttcca agaatcagca
     4621 acaaagaaat atcctggatt ataataataa gttgcacgat aattcgagta aaaatactca
     4681 tcagcttctc ccagataaga agaaatttct gccacaattc ccatttcttc tatcagctca
     4741 cggacaatcg cttcttcttt cgtctcttct ccttcaattt ccccgccagg aagaaaaaaa
     4801 gcaccatttg gagcctcgat tactgcaatc tgctgatctc tttctatgac aatatatgct
     4861 gcataacggg tttgataatc agcaaattgc tttttttcac caaaaattgg tttatccatg
     4921 aatagtttcc accttaacag ttttattttc tatccatttt acatgagaaa tttctcatga
     4981 acaagcacaa atcaactatt tccttacttc tttttgttat gattgagtaa aaggaaaagg
     5041 agagatgaat ctatgaaaat ggctcacaca tgtgttcgag tgaaagatct tgaagcatca
     5101 atcgaatttt atcaaaaggc atttggtttt gaagaaagcc gccgccgtga ttttccagaa
     5161 aacaaattta cacttgttta tttgacactt cctggggatg actatgaatt agagttgact
     5221 tacaactatg atcatggtgc gtatgatcta ggagacggct atggtcatat cgctatagca
     5281 gctgatgatc tagaaaaatt acacgagcaa cataaagctg ctggtcttga gattactgac
     5341 ttaaaaggat tacctggcac tgcgccttct tactactttg tcattgaccc tgatggttat
     5401 aaaatcgaag tcatccgagg atagttagta tggggcattt tactcttgat tgaataaaaa
     5461 acaaacacca ataattaaaa tgagacgcaa acttttcatt aaattgaaaa agtttgcgtc
     5521 tttttttaac aaccattgta gctataaggt tgccactctc caccaattta tttttaattc
     5581 atttgaaaga aatcaaataa acgaggtacc tttagaaaac aaaactgctt ttcctcctat
     5641 tatcacacga tcttctttta attcacattc tagatccccg ccccttgatg acatttgata
     5701 agctgacaat tttgttttat tcattctttc tgcccaataa ggaataagat tagaatgtgc
     5761 tgatccacat actggatctt cattgattgt taattttgga aagaaggttc tagaaacaaa
     5821 gtctttttgc acactttctg ctgtcacgat gactcctatt cctaattcaa aatttttgat
     5881 agctgtaaaa tctggctgca atttagccac cgttttttca tcttctaata cgaaaaataa
     5941 atcacgtgct aagtaggctt ctttaatctt agccccaatc gcttcttcat attcagacaa
     6001 tatcggcact tttttcggca taatactagg aaaatccatg taataataat ctttaattct
     6061 tgtcacaaat agattaccgc tttgagtaga aaatttaatc gtttcatcag gtattttgta
     6121 atagttaaat aaaacaaatg ctgttgctaa tgtcgcgtga ccacaaagat cgatttctcg
     6181 atctggcgta aaccatctaa gctcaaattc ttggtttttc ttcactgtaa atgctgtttc
     6241 agataaattg ttttcaatgg caattttttg catcgtacct tctggcagcc attcttctag
     6301 cacataaacc gctgctggat ttcctttgaa cacctcatct gcaaatgcat caactacgta
     6361 ataatctaag ttcattttaa agcccctttc tctaatttgt tataaatata ttctgcacaa
     6421 caaagatctt caatagctag accaactgcg tcgaaaatcg tcggcctcga ttctccttta
     6481 taaaatgttt ttttctcaga aactaattcg cctaatgtac caacaataca agattcctct
     6541 aaatctcctt ctgccatggc tataagcagg tccccgcttt ctgttaaggc agctgagtaa
     6601 tcatctacat agatttgaga agtttccatc aacgatgtac ttatctctct tgtatcagga
     6661 gtaaatgtgc ccacagcatt gatatgtgcg tcttccctta cccactcttt ttgaagaaat
     6721 gcctctttac tagaggtcaa cgtacaaatg atttccgctg gacgtactgc atcttctact
     6781 gatattgcat ttataaacgt tattttagga tactgttttt tagctttagc gataaaagta
     6841 tttcgtcttt cttctaaaat atcgtaaaca aacactgtag tgatgggacg aatggtagaa
     6901 atcgcagtca aatgcgacaa tgcttgttga ccagctccaa ttaaagcaag tgtttctgca
     6961 tgcggatttg ctaagtaatc tgttgccagc gcagatacag cagctgttcg aatccaggtg
     7021 actgcatttg cgtttatcaa tgctttgggt agtccgtttt gagcatcaaa caagaaaatc
     7081 tctcctaaat gagaaggtaa atgatactga tgattatccg gaaaagcggt aatgactttt
     7141 gtgccaaaat atctgttctc ccccaaataa gcaggcatca tcgcaaacat atttttgcct
     7201 tttccgtcag gtaaaatttc caccaatcga ggattttggc tgattttacc actttcataa
     7261 tcaaaaaaac attttttcat tacttgaatc gcttcttcaa aactaaaaga agctactact
     7321 tcctcaaaat taataatttt catagacaat tccctcctca caaaagatta acttctaaaa
     7381 tcaatacaat caatggtaaa attgtatggt atacaatttt aaggagtgga gaaaatgcct
     7441 attaattctt atgacaatta cccgttaact tggcgaccag acaaacaaaa attgaagcgt
     7501 ccttattatc gatcgttgat tcaacaatta gaacaagata ttttaaagaa taaattacag
     7561 aaaaacactc agttaccctc ccaaagagag ctagctgatt atttagattt aaattttact
     7621 accgttggca aggcttataa atatggaatt gagaaaggtt tgctgtatac gaatattgga
     7681 agtggcacat ttatttctcc caatgtcttt gagtccatca ccatctctac caatgaagta
     7741 accgaacata tcattgattt tggtctggtt agttcttttg aagaatgtaa taaattcgtt
     7801 gtcccgttta ttcaatcaac tagtaataac ccaaatcttg ttggtttgct taattaccaa
     7861 gatcccaaag gcacaaattt tcaattgact actgcagcaa attggttaaa aactcaagga
     7921 gtcattccgt ccttagaatc tctatctatt gtttccggtg ttcaaaatgg attggctatc
     7981 acactttctg cacttttctc tccaggtgat agaatagcaa cagaccgata tacatacgct
     8041 aatttcattg aattggctca gttatatcat ttagagatcg ttccaattga ttttgacgag
     8101 caaggaatgc gacctgatat attgattcag gaatgtagaa aaaagaagat tcatggtgtc
     8161 tttcttatgc cttcttgtaa taatcctata gggtttcaaa tctctcacaa aagacgagag
     8221 caattagccg aagtgattga aaaagaaggt ctatgggtta ttgaagatga tatccattca
     8281 tttttaacaa cttactatca acatacgatc attcctcctt tccaaaattt attacctaac
     8341 caaacaatct acttagctgg aatgacaaaa tttatttgtt ctggattgcg agttgcttat
     8401 cttgtagttc cagtacatgt ccaaagcaag atagaacaag caatttttaa tataaatgtg
     8461 aaaacttctg gattagatgt ggaagtcatc actcaaatcc tccattcaaa tgaagcaaat
     8521 aggatcttag aaactaaatt aagtcttaca aaaagagcca atcagctttt tgattctctt
     8581 ttcaaagtag aaaaaccaac gaatcgtgtc ccttactatc gaattcttcc agttgatcct
     8641 gagattgacc ctaagataat tgagaatgac tttcttgcta acggtatccg actttatcat
     8701 tccagacgct ttactacacg tacgcaacca gatgcattcg ttcgaatttc cctatcctct
     8761 aataaccttg aaattttagc ttctggacta agtatcgtaa aagaaaaaat aaaaaaatat
     8821 gaattataac ctattga
//
LOCUS       DANKKG010000114         8613 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000114 DANKKG010000000
VERSION     DANKKG010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.114"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(211..2592)
                     /locus_tag="PW004_002230"
     CDS             complement(211..2592)
                     /locus_tag="PW004_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HDL2727184.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2582..3229)
                     /gene="recU"
                     /locus_tag="PW004_002231"
     CDS             complement(2582..3229)
                     /gene="recU"
                     /locus_tag="PW004_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HDL2727185.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3293..3832
                     /locus_tag="PW004_002232"
     CDS             3293..3832
                     /locus_tag="PW004_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HDL2727186.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3922..4341
                     /gene="gpsB"
                     /locus_tag="PW004_002233"
     CDS             3922..4341
                     /gene="gpsB"
                     /locus_tag="PW004_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HDL2727187.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4363..4730
                     /gene="rnpB"
                     /locus_tag="PW004_002234"
     ncRNA           4363..4730
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PW004_002234"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4939..6105
                     /locus_tag="PW004_002235"
     CDS             4939..6105
                     /locus_tag="PW004_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HDL2727188.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6151..7647
                     /locus_tag="PW004_002236"
     CDS             6151..7647
                     /locus_tag="PW004_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HDL2727189.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7728..8411
                     /locus_tag="PW004_002237"
     CDS             7728..8411
                     /locus_tag="PW004_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL2727190.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
BASE COUNT         2705 a         1566 c         1830 g         2512 t
ORIGIN      
        1 ttataaacaa gtaaatggat aaagtatatg taatgataaa agaaaaatgg attttctaat
       61 ttttaagcta aatgttagat cacacatgta tatgctggat agtcttataa aaaatcatgt
      121 aatacagctt ggtacatgat ttttatttgt agctgttttt tacatagaca caaaaaagaa
      181 accacgcttg tagacgtggt ttctttttta ctatctgttt ccagaagctg aggtgctgct
      241 ggatggtgta tggtcatttg cagcatccgc tccgctagtt gctggctcac tggaagaagc
      301 aggaggagca gatgaagacg gtgtcgagct ttcttcggat gaagaagaag gaaccgttgt
      361 tgaagacgct ggctcgcttg tttctgctgt cgtgctttct tttgaggact cagcagttga
      421 ttcgctttga ctagtagagc tagagctttg agtcgttgtc tctgttgttg agcttccagg
      481 agtctggaca tagctggaag atggaatagt tgttgatggt gtaattgcat tcgatctagc
      541 tgtatattga tctttgtagt acagttcacc gcccacacga attaatccgc ttggcatctc
      601 ccagtctgta tttgtcacgt tagctgaaac atactgcata agctcgcgat aaacatcaga
      661 ggctacgtgt gacgattctg aagtgacagg cgtcattttt ttattgtaac ctgtccacac
      721 ggaaatcgaa taatttggcg tatagcccgc aaataggata tctggataca ccccagaaga
      781 aataccaagt tttgcatact cgtcatctgt gtaattggat gttccggtct taccggcttg
      841 atataaccca gcgatttgcg cattcgtacc agttccttca gtgatcgtat cttttaaaat
      901 gtctgtgatc atataagctg tttctggaga catggctgtt tttccatctg gctcataagt
      961 ttcttctgtt ccgtcttgga agacgatttt attaacatat tgaggtttgt aataagtccc
     1021 tccattggcg aaagccgcat aggctgctgc catttttaag gaagaagctc cgtattttgt
     1081 cccatcttgt tcttccgtat tactagagat cgcattcgat tgatggatcg tactgtattc
     1141 gatcccaaga ttttttaaga aagaagccac tttatctgaa ccgacttcat tgaataattt
     1201 gactgctgga acattacggg ataagtaaag tgcttggcgt aaagtaatgg tgcccatata
     1261 ttgattatcc cagtttccta caggagtcga agtcccttca tagttatacg gtgcgtcagt
     1321 aatagttttt ccagtagaat atttcaaata ttcaaaagct ggtccgtaat ctgtgacagg
     1381 tttcatcgta gaaccaaaat cacgggaagt attcactgct aagttgttgc ctaaagttac
     1441 gtcttctgcg atgtgacgtc cgccaatctg ggcttttact ttcccggtat tggtgtcgat
     1501 aagggtggag gcaacttgca tctcgtcgtc tgggtaagag acatactgat ccgtgttgac
     1561 gatatcatat agtttctttt gggcgtccag atccaaattc gtataaatct ctaaaccgtc
     1621 tgtgtagaca ttcttatctg ttttttcttg tacttcattg atgacttctt tgacataatt
     1681 atcgacgatc ttagtattgt catcggattg agttagttct tgcagaccat ccgttaccgg
     1741 tacattaaca gcttgatcat attctgtttg agagattttt tcgttttgaa gcatcgtata
     1801 caaaacagta tctcgtcttt tcttcgcctg atctggataa acataaggat cataggcgga
     1861 aggggcttga ggcatcccag caagcaatgc tgtttgcggt aaagaaagtt ctgataattt
     1921 cttaccaaag tacatctcag aagctgtctc catcccgtac aagccgtttg acatatacac
     1981 tttatttacg tagtaggtca agatctcttg tttcgatttt ttttgttcca atcttacagc
     2041 catccacgct tcttgggcct tgcgtttcag agtctgatcc tcggcactgg tggagaagaa
     2101 ggaaagtttg atcaattgct gtgtcaaagt acttccgcct tgcagtccgc cagaggtaaa
     2161 gttagagagg gcagaaccga tgatccgaat agggtcaacc ccgatgtgtt tataaaaacg
     2221 gcgatcttct accgacacga tcgcatcttc taatgttttc ggtaattcat tcgcagaaat
     2281 tttttcacgc ttttctgcgc ctaaatcttc gaatagttcg ccatcttgtg tatatagttt
     2341 ggaagatacg gtagcgtcta attttttatc cgtcaattcg ggcgcatctt ttgcgtaata
     2401 ccagaaaagt cctacacctg ctaaaaagag gatacagaaa aatgagagta ttcctaaaag
     2461 gatcttgata aataatcctt ttttatgtgt tccagaggac tttccagaac ctttgccaga
     2521 attcttcttc acactctttt taggagtagg ctgtttctgt cgtctagagc ttctagtttg
     2581 ttcatttgcc attcttgctt tctccttcaa tagataatgt tgataaatag aagtcaaccg
     2641 cgtccaaata aggaatccgc ggagaaatac cagttttgat ctctattcct agttcttcga
     2701 tcaaagataa aggtagtgat ttgcgtcctt tttccttttg ggcgtcccag taagcaatca
     2761 aatgaacact attcaaataa aaacagcgct tcaaagacga aaaccataac aagacaaagc
     2821 agaccccatt ttgttcgagg catgctttca tatgttcgat ttgatgggca tggaagtttt
     2881 taaagggaaa agatgttttg ttcttggttt cttttgcttc aaagtccaag taaaagcctc
     2941 gatagacgcc attatagtct gtagttgagg cttgggtaaa gtaggcttcc ttgatcacag
     3001 cagcacttct cttaggatag tctactttca cgatttggat aggggtaggc tttttatgga
     3061 taaccgccaa cccgcgggaa agataatatt tattactttc attgattgct tcttcaaagc
     3121 gcatcccccg attgccgaat tcagtgatct gatttctttt ttcttgaatt ttttcgacgg
     3181 gagaggcatt cttataataa ggttggccat ttggatagcg caataccatc tttaatcaca
     3241 ctcctaggcc acattatagc aaaaaagcgg atagaaagaa aaggaatctg agatggataa
     3301 gtcaaaagta atctatgtgt ctgggtatcg aagtttcgaa ctcggtgttt ttaaagaaaa
     3361 tgatcctaaa atccaagttg ttaaaaatgt tttaaaaaaa gaactccatc agttagcaga
     3421 agaagggctg gaatgggtac ttgtcagtgg gaatttgggc gtcgaactat gggctgtgca
     3481 aatagtagcg gaattgaaag aagaatatcc ggaactatct cttggtattt tgtatccttt
     3541 ttctgatttt ggtaataact ggaatgaagg gaaccaagaa aaaaaacaat tagcagaaca
     3601 attagcagat tatgtcgaag ctgttagcca tcaaccctac caatcaccta gtcagctaaa
     3661 gaatcacact cgttttatac ttgagcatac agtgggctgt ctgctgatct atgatgaaga
     3721 attccctgga aaaacgaagt atttttttga agatgcacag aattaccaag cagatcatcc
     3781 ctacgaaatt cggctgatca gtatggacga tcttcaaaat tttagtgaat aatcgatatt
     3841 gtgtttgatt tatcaacttt tttcctatac aatgaaaaag gtagaaatgt tattacgtaa
     3901 atgaaaacta gaggtgtaaa tatggcgaat ttggtttata gtcctaaaga cattttacaa
     3961 caagaattca aaacaaaaat gcgcggctat gatcccgttg aagttgatga attcttagac
     4021 aatatcatca aagattatga aacttacagc aaagaactcc ttgctttaca agaagaaaac
     4081 gatcgattga gtgcaaaagt agctcaatta tctaaaactc aaggagcagc tcaaacacgc
     4141 gtgcaacaaa cagaggcacc aaagagcgca gcagtgacca attttgatat cttaaaacgc
     4201 ttgtcaaatc ttgaacgtga agtatttggt aaaaagcttg atcaacaagc aagtgcagtc
     4261 aaaccagcac aacctaatcc aaataactac acaaatgcag acacaagctt agatgataat
     4321 gaaaaaacac gtcaatttta atttttgatt aatgaatttg cggttttcgg gtaatcgcgg
     4381 tctgacttga tcaggctgag gaaagtccat gctcgcacag gctgtgatgc ctgtagtgtt
     4441 cgtgcttagc gaaaccataa gctaaggtac tctttggagt aacggcagga aaacaagcta
     4501 aggcttcggc tatgcttaag tatccttgaa agtgccacag tgacgaagcg gctggggaaa
     4561 cccatccggt ggaacgcggt aaacccctcg agcgagcaac ccaaacaatg gtaggggcgc
     4621 tttttgtctg gaagaagaac aggacaaaaa ggcagtataa ctgcagatag atggttaccg
     4681 ctttgttttt tctccctgga aaagcaaagt acagaacatg gcttatagaa aaccgcaaac
     4741 atcagggaat taggtcgtga caaaagtaat gcgttataag aaacaagaaa aacatttgaa
     4801 agctattttt caatacttca gatgaatcgt tcttattaca aaacaaactt ttgtatcacc
     4861 ggttattcgg aatttgagta aaagtgtggg gtaggactga tgtcccacct ccttttttta
     4921 taaataagga gattgtgtat ggaaaaaaac aggaagttca acctcgtcgc aactgcggca
     4981 agcgggctag aagcactggt tggtaaagaa ttaagagatc ttggaatcga atgccaagtt
     5041 gagaacggac gtgcacgttt tgaaggaaca atggaaacga tcgctacagc taatttatgg
     5101 ttacgtacag cagatcgagt aaaaatcgtg gttggggaat tcgatgcgta tacgtttgat
     5161 gaattgtttg aaaacgtaaa agcattgcct tgggaagatt ttcttccact agatgcagct
     5221 tttcctgtag ctggaaaatc gatcaagtcg aaactatata gcacacctga ttgccaagcg
     5281 atcacaaaaa aagcaatcgt tgaacgtctc cgtacctatt atcaccgtcc agcttctgta
     5341 ccactgacgg aaactggcgc acattttcag ttggaagtag ctttgctgaa agaccatgtg
     5401 atggtaactc tggatacgac aggaccaagt ttattcaaac gtggttatcg cttagaaaaa
     5461 ggtggagcac cattgaaaga aaacatggca gcagcactta ttatgttgac aaattggcga
     5521 aaagaccgtc cattttatga tcctgtatgt ggttcaggta cgctttgtat tgaagcagcc
     5581 ttaataggcc ataatatcgc accaggattc aatcgtgagt ttgcttgcga atcttgggat
     5641 tggttcagtc aagaaatcat ggaaaatgtt cgatcagcag ccgaagaaaa agcagattat
     5701 gatattgaat tagatattac tggttcagat atcaatggac ggatgattga aattgccaaa
     5761 gccaatgcag aagaaatcgg attaggctct tcgattacct ttaagcaaca agcagtcaaa
     5821 gacttcaaaa cggataaaga atacggggtc atcgtggcta atccgcctta cggagaacgt
     5881 ctaggagaag aagaaactgt tcggaaatta tataaagaaa tgggcgatgt attccgtcca
     5941 ttaaaaacat ggagtaaata tatcttgact agcgacttag cgtttgagga attttacgga
     6001 caaaaagcaa ccaaaaaaag aaaattgtac aatggtgcat tgcgtacgga tctattccag
     6061 tattggggaa ctcgtccgcc tagaaaaaaa gagaaagacg aataatctta ctaattgatt
     6121 cattcaattg gatgtttgga ggaataggga atgaaagaaa aagagttttt acaagaagta
     6181 aaagaaatca atcttctaca acaagcttta ggaatcctcg attgggatac acaaacaggt
     6241 atgcctgaaa aagctagtgc ataccgaagt gaagtagaca gctatcttta cagcatttat
     6301 ttttcaaaaa aaatcggtcc aaaaatcaaa gaagccattc gctatttttc tgaacatccg
     6361 gaagaattgt cggaagttgg aactgccgtt tttgccaaag taaaagaaga gtatgatttg
     6421 gaacatcgcg taccggaaga tcttatgaga aaatatacag ctgccgtttc ttcagcacac
     6481 ggacaatggc aaaaagctag agaaacacaa caattttctg attttcaaca cgcattgaat
     6541 caaaatattg aattaacaaa acagttgata ccttactgga gaaaagatga aaagacgaat
     6601 tatgatgttt tgctgaatca atacgaaccc gggatgactg tcgaaatcct tgataaggta
     6661 tttgatcagc ttaaagaagg aattctttcg atccgccgta cgctccaaga aaaagggact
     6721 gaacctgaaa cagacttttt aaatcggaga atgacgaaaa aacaacagaa gagatttgta
     6781 gtgaaagtaa tcgaacaact tggttatgat tttagtcgtg gtcgtttaga tgacactgtc
     6841 cacccattta tgactggaat taatccgaat gatgcacgaa tcacgacacg atggaacgaa
     6901 acagatttca aaatggctgt ttttggagtg atccacgagg ctggtcatgg tatgtatgaa
     6961 caagacatcg atgataaata tgcgtataca ccggtttatg aaggtacatc aatgggtatc
     7021 catgaatcac aatcgttgtt taacgagatc attattggaa gtagtcgcag cttctggaaa
     7081 aagcaatatc ctttctttca agaatgtgca gaaggaacat tcgatgatat tccttttgac
     7141 aaattttatc gttcattgaa ccatacaaaa tcaagtttgg ttcgtatcga ggcagacagt
     7201 ttgacttacc ctctccatat catcattcgt tatgaaatcg aaaaaatgat tttcaatgaa
     7261 ggggtagatg ttgctgaact acctgaaata tggaatcaaa aatatgaaga gtatctggga
     7321 atacgaccaa caaatgatgt ggaaggcatc ctacaagacg ttcattggtc aggtgcaagc
     7381 tttggttatt ttccctctta tgcattaggg tacatgtatg cggcacaatt acttcattct
     7441 ttgcagcaag aaatagatgt ggaaaatgtt ctagcgtcag acgattattc tccaatcaaa
     7501 aactggatgt cagagcatat ccatcaatat ggtgcatcta gaaaaccaaa tcaattaatc
     7561 atggatgcaa cacatgaacc gcttaatccg caatacctga tcgactatat gaaagacctg
     7621 tattttaatg tatatcaggt aaaataaaat acaatttaca aagatagctg aaattcatct
     7681 aatctataga tgttttcagc tatttactta agaaagaagt ggaacgaatg gataaattac
     7741 aagtgatcgc tgattatagt atggaccaat tagcgcatga tcaaacagga catgggagcg
     7801 atcataccaa aagagttgtc aagttagctg aaaaaatttt ggagacggaa ccgcaggcag
     7861 accgctttgt gacgcttgca gcagcttacc tacatgatac gattgatgat aaagtcgtta
     7921 aaaatgagaa cgaggcaaag caacagcttc gtgtattcct tcgctctttg ccaatagtag
     7981 aggaacagat ctcgatgatt ttttccatta tcgaaaatat gtccttttct aaaaatctct
     8041 ctgaagcggc cgaactatct ctggaaggta agatcgttca agacgctgat cgtatcgaag
     8101 cattaggagc aatcggcatt ttgcgtactg cttatttcgg cggtggacat gggcacccga
     8161 tttttgatcc tgaactgtat ccgcaaacat tcaaggacaa agaaaattac cgaaaaggca
     8221 cgacagtgat caatcatttt tatgaaaaac tgttcttgct gtctgataaa atgaatactg
     8281 attacggata tgaagaagcc aaacgaagag aaaccttcat gcgtgaattc ttagaagaat
     8341 tttttctgga atggtctatt gacgatgaaa attttactcc agacaattgg atcaaaaata
     8401 acaaaaaatg atggaggttg tgacagaagt ggtcagctcc aagatatttc ggatcatttt
     8461 ccgtaaaagt tacttctgga acactgttta ccaagaggtc gtgagcctgc cgttcagctc
     8521 cgagtcacaa ggaacaattt caaaaaatag cttctcgtgt ttgatgaata ttgtaacttg
     8581 tgattcggaa ttggcaggag aacaccgttt att
//
LOCUS       DANKKG010000115         8372 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000115 DANKKG010000000
VERSION     DANKKG010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.115"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            251..1300
                     /locus_tag="PW004_002238"
     CDS             251..1300
                     /locus_tag="PW004_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2727191.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            1297..2178
                     /locus_tag="PW004_002239"
     CDS             1297..2178
                     /locus_tag="PW004_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2727192.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            2181..2951
                     /locus_tag="PW004_002240"
     CDS             2181..2951
                     /locus_tag="PW004_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2727193.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            3230..4084
                     /gene="aroE"
                     /locus_tag="PW004_002241"
     CDS             3230..4084
                     /gene="aroE"
                     /locus_tag="PW004_002241"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDL2727194.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            4262..5425
                     /locus_tag="PW004_002242"
     CDS             4262..5425
                     /locus_tag="PW004_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDL2727195.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            5542..5952
                     /locus_tag="PW004_002243"
     CDS             5542..5952
                     /locus_tag="PW004_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL2727196.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6126..8354
                     /gene="topB"
                     /locus_tag="PW004_002244"
     CDS             6126..8354
                     /gene="topB"
                     /locus_tag="PW004_002244"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDL2727197.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
BASE COUNT         2888 a         1447 c         1825 g         2212 t
ORIGIN      
        1 aaaaaaaatt ggcgcagctg gcgtagataa gttgtattaa tggtgagact ttcccataga
       61 tacaactttt tagagtgcat ttctaaagct tgtatctatg ctagacatga acattgactg
      121 ccgcatagat tagaatctat gcggtttttt gtgtaaagac aatgaccgct ttggccattg
      181 tctttttttg tagactggat acttgcctga ttataataaa taaaaagatt gactttgcag
      241 gaggaaaaaa atgaaaaaca aacgattaat aacagttgta gtaattattt tagtttattt
      301 agcaggtgca tttatttatg gaaaagcctc aggcgaaaaa gaaagtactt cgacgaacca
      361 gacaaaaaaa acaacagttg ttggggtatt gcaatacgtg agccatccgg cattagatga
      421 gatttatcga ggaatcaaag atggtctgaa agaagaagga ttaaaagaag gagaaaatct
      481 tgagatcaaa tttcaaaatg gacaagccga tcagagcaag ctagccacaa tgagtcaaca
      541 gctagtacaa tctgatccag atgccttgat tggaatcgca acacctgccg ctcaatcatt
      601 agcaaacacg acttcatcga tcccagttgt gttaggtgct gtaacggatc ctgtgggggc
      661 tggattagtc gcttctttag agaaacctgg tggtaatgta acaggtgttt ctgatcagcc
      721 gcctgttact tcacaaatca agcttgcttc tgatttgttg ccaaatgcaa aaaaagtcgg
      781 aatcttgtat gcttcttcag aggataattc aaaataccaa gtagcacaag cagaagaagc
      841 tgcaaaaaaa gaagggttag aatcagtaaa atatgcggtg ccttccacta atgagattgc
      901 acaaacggtt caagtgatga gccgacaagt cgattttatc tatgttccat tagataatac
      961 cattgccaat gcgatgcaaa cagtggtaaa agaagcaaac aaagcaaata tccccgtcat
     1021 tccatctgtg gatacaatgg tggaacaagg tggactagca acaatcggga tcaatcaata
     1081 tcaattaggg cttcaaagcg gtaagatggc ggcagaactt gcgagtggaa aagaaaaacc
     1141 ggaaacgacg cctgtttata catttgatca aggggataca gtgatcaatc aatctcaagc
     1201 tgatcgctta ggaatcattg ttccccaatc gatgaaggaa aaagccaaaa tcattacaga
     1261 tgactcgcag caagaaacat ctaaggagga tgacaaatga tcgtatcagc tatttcacaa
     1321 ggcatgcttt gggcaatatt aggattaggg attttcatga cttatcggat cttagacttt
     1381 cctgatatga cgacagaagg gtccttccca ttaggtgctg ctgtctgtgt gacagctatt
     1441 actcatgggg tagcgccgat tgtagctaca ttattaggcg taggtgctgg catgatagca
     1501 ggactcgtta cggggttact ttatacaaaa ggaaagatac cggttatttt agcagggatt
     1561 ttagtgatgt caggattgaa ctcagttatt ttgtttgtga tgcgaacacc aaatctttct
     1621 ttattgaatc atccgattct acaagacagc ttccatcgct ttgggttacc tgattacttt
     1681 gacacagtcc ttcttggctt catctctttg ggcatcttga ttgctgtact gctattcttc
     1741 ttcaatacag atttgggtca aggctatatt gcgacaggag acaacgaaac gatggctcgg
     1801 tcgttaggga taaaaaccga tcgtatgaaa attttaggtt taactttgtc gaacggtgtc
     1861 attgcattat ccggtgcgct gattgcccag aacgacggat atgcagatgt aaataaaggg
     1921 attggcgtaa tcgtcatcgg tcttgcttcc atcatcatcg gtgaagtgat cttccaagaa
     1981 ttgacgctag cagaaagatt gattgctatt gttgtcggca gtatcattta tcagctattg
     2041 attttagtag taatcaaact agggtttgac acgacttatc tgaagttgtt ctccgctatc
     2101 atcttagcta tttgtctgat gatcccgcaa ttgaagaaag cactgaactt aaaaacgggt
     2161 tttgagaagg aggcatagta atgactgata tattgacaat caaaaatggc agaaaagtcg
     2221 taaacaatgg aatgaatgaa gagaaagtac tattggatga aattaacctt acaatcagag
     2281 aaggggactt catcactgtc cttggcggga atggtgctgg aaaaagcaca ctgttcaata
     2341 cgatcgctgg tacgcttcaa ctcagtcaag gatcgatctc ttttaaagat cagctgatca
     2401 caaaagattc agaagaaaaa agagcacgat tcctttctcg agttttccaa gaccccaaga
     2461 tgggaacggc tcctcggatg acagtagcag aaaatttgct acttgcacaa aaaagaggtc
     2521 aaaaacggaa attccgttta cgtaacttga aaaatcaaaa agaagatttt ttcagactat
     2581 gtcaagaagt cggtaatggc ctagagcaac acttggatac tccagcgggt gaattatctg
     2641 gtggtcaacg ccaagcatta agcttgctga tggcgacgat cacagctcct gagttactat
     2701 tattagatga acatactgca gctttagatc ctaaaacagc taaagcatta atgcgattaa
     2761 caaatcagcg gatcaatgaa caaaaattga cttgtctcat gatcacgcat cgaatggaag
     2821 acgccttgaa atacggaaat cgtctgatcg tactccaaaa agggaaaatc gttcaagata
     2881 tttctgaaaa agaaaagcaa catttgacaa tgggcgacct tttgcagttc tttgaagaag
     2941 tagtggattg atttcgacag aaaaattcct ttcagtagaa ggacttgaaa actcattgac
     3001 atcttccttt gattcatcct atacttaaaa caataaacaa ttaaacatat ttgggtgttt
     3061 tgtaggatca cgtagtcaat gattttaaca attaaataga atattctttg ctctgacaag
     3121 taaacgatga gaaaagaatt tgctactttg ccacctagac gtttcgtact ttttcgtatc
     3181 aaacgctaag tggctttttt tattgaagta agaacaaagg aggaataaga tgatttctgg
     3241 acaaaccaaa ttagctgggc tctttgctga tccggcttct cacagtttat ccccattgat
     3301 gcacaatact gcttttgaag caaacaacat cgatgcggtg tatttagctt tccaagtaaa
     3361 tccaatgaat ttgaagcaag cagtcgaaag tattcgaact tttgatatgt taggtgtaaa
     3421 tctttcgatg ccgaataaaa cagcggttat tccttatctg gatgaggtaa gccaggaagc
     3481 gcaattgatc ggagcagtga atacgatcgt gcacgaaaag ggtcgtttga ttggctataa
     3541 taccgatggt gcgggcttca tgcaatccgt gcaagaagct gggatttcga tcaaaaagaa
     3601 aaaaatgact ctactgggag ctggaggtgc agcaaaggca gtcactgttc aagctgcgtt
     3661 agacggcgct gaggaaatcg tagtttataa acgaaacaac caaacattcg ctcaagtaaa
     3721 agaaacattc gaacaactca gctatcgaac ttcttgtaag ataaaagttc gtgactttgc
     3781 cgatacgcat caattaagaa aagacttgaa agaaagctgt cttctagtca atgcaacaga
     3841 tatcgggatg ggaaagaaaa tcaatcagtc accgctctta gatataagca tgatgcatga
     3901 gaatctagcg gtttttgatt tgatttatgc acctagagaa acacgtttat taaaagaagc
     3961 aaaaaaagta ggagcaaaag gatacaatgg acttggcatg ttgatctacc aaggagcaat
     4021 cgcgtttgaa ttatggacaa aggaaaagat gccgatccat gtgattgaaa agctgtttga
     4081 ttaaaaaagg ataatagata aagatctttt attctcgcat aaacggtctc aagaaaagta
     4141 atcctttatg tatcgaagca tttatttcgt tattttttat taaattatca aaaaatgata
     4201 ggcttttgtc gattggttat cgtatccttt atttatcagt aagaaacgag gaggaaccac
     4261 aatgagtcgg atggatcgct ataaggacat ccataagaag gcgaaacctc tgaagcggga
     4321 tacggggagt atttttgcta gaagagaacg aaaacgaacg cgtatggaaa atgaggaaga
     4381 tgctacccgt atttatgaaa gagagcagca ttcatttgaa catttgaacc aatacgagca
     4441 acagatatac agagatgcac caagagcaga agaacaacaa aaaaataaaa aatttttcaa
     4501 tcggcaaaag aagaacaaac cgttaaaacc tcgaagaaag cgatcgtgga aaaaaatcat
     4561 tctatcgatt ttgcttgttt tgatccttta ttcaggtatc tcatttttta ttggtaaata
     4621 tgtagctgaa catgacacaa gcttagaggc agcgacttca gaaacctttc atgggcaaaa
     4681 agcgacgaat ggtgcgcata atattttgat ccttggaagt gatacacgcg gagaagatgc
     4741 cgggcgagcc gatacgatca tggtcttaca gttggatggt ccttcccata agccgaaact
     4801 gatttctttt atgcgtgaca gcttcgttac gatcccaggg gtaggacaaa ataagatcaa
     4861 ttcagcttat gcatacggag gagccgatct agtgcgtcaa actttagtag aaaattttgg
     4921 aatcgattgt caatactatg cgaaagtcga tttcaaatcg tttgaaaaag tgatcgatgc
     4981 cttattcatg aacggagtga agatcgatgc agaaaaagat ctgaatctgg atggtgtaga
     5041 tattaaaaaa ggggtccaga agatggatgg acatgttctt ttgcagtacg cacgtttccg
     5101 aatggatgaa caaggagact ttggccgtgt cagacgccaa cagcaagtaa tgaacgctat
     5161 tttcagccaa ttgaagaatc cattgaatct gatccgtgca ccttatgcag ctggtaaagc
     5221 tttaggatat acatccacag atgtcactgc ttggttcttg acgaaaaacc tcttttcgat
     5281 tgcacgagga gctggtggca ttgatcgaat aagtgtgcca gtagacggtt cgtggaatta
     5341 tgggcaaagt gattatgctg gcagtattct tgaaattgat aatcaaatga atcaatctgc
     5401 tattacagat tttttgaata aatgatcata aaaaagatca agaagaagct gcgcaaggct
     5461 tttgtcatga atcaatcaaa cgattcactc gtaaaaaacg ctttcttatg gtagatttaa
     5521 ccaagagagg agctgtttac attggatatc tatctgaact tcaaaaatga gtcgcgtgaa
     5581 gccatcgctt tttatgaaaa agtttttcag actgaagcat caggtatcac cacatacggt
     5641 gaaatgcctg gaatagaaca aatgaacttg ccagaagaag caaaaaatct aatcacgaat
     5701 gcacggctga acatttatga tacattagtt atgtttgcag atacgccaga aggaattggt
     5761 cctgaattag ttgtgggaaa taatgtttct ttagtcatcc agacaagaaa tgaagaagaa
     5821 acgaagcgcc tatttgaaag cttagctgag ggtggtaaga ttgagatgcc actggatgct
     5881 gttccgtggg ctcagttgta tgggatggta actgatcgat ttggcatcaa ctggcagatt
     5941 aaccaagagt gacagctaaa gatgaatatt ttctggaaac atcaagcagc aaacggatca
     6001 tcgtttgttt gcttgatgtt tttgtgtcta tttcgactat acggtatctt ttttttattg
     6061 ccagatgcga aaatatgttc cataatagag aagtccgcaa aaaattaagt aacggaggtc
     6121 agaagatgaa gacggtgata ctagctgaaa aaccttcaca agcaaaagcc tatgccgaaa
     6181 ccttccaaca gcaaaagcgg catgaagggt attatgaaat cagcgatcca ttgtttgctg
     6241 gtgaagtaac catcacttat ggttttggac atttggtaga tatggttgct ccaggagctt
     6301 atgaagagcg ttgggcaaaa tggtctttag aaaatttgcc tattttccca gaaaccttcc
     6361 gttacgaagt tccaatggac aaacgtgcac aattttcgat cgtaaaacga gaattgcagt
     6421 cagccgatac catcattatt gcaacagatg gtgatcgaga aggtgaagcc atcgcgtggt
     6481 cgattattcg acaagcaaac gcattttcta aggaaaagaa ctatttacgt ttatggatca
     6541 attctcttga aaaagaagca atctatgaag gttttaagaa tctccaacct ggaaagaact
     6601 attttccaaa atataaagaa gcgcaagcaa ggcagaacgc tgattggctg attggaatga
     6661 atggaagtcc attatatagc ttgctacttc aacgaaaagg gattgatggc agtttttctc
     6721 ttggtcgtgt acaaacaccg acactctata tgatttatca gctgcaagaa gaaatcaaac
     6781 acttcaaaaa agaaacgtac tttgaaggag aaggtttaat tacgacaaac aaagggcagt
     6841 tttctggaaa aatcgtacca aaaaaagcat ttaaaaccca agaagagtta acgaaggaaa
     6901 tagaaaaact tggagcgcat ttaggtaagc aaaacggacg gattcttgaa gttacgaaaa
     6961 aggaaaagag gttgcatagt ccacgtttat tttcgctatc tagtttgcag acaaagatga
     7021 atcagctaat gaaagcaagt gccaaagcta cacttgatgc tgctcaagga ttgtacgaaa
     7081 gcaagtttct aagctatcca agaacggatt ccttttacat tacagaaaat gagcatcagt
     7141 atctggttaa acatctgcaa aattataaaa aatttttagg aatagaagaa gtacagacaa
     7201 ctgagatcaa agcaaagaaa cgatatgtcg atgcgaaaaa agttcaagaa catcatgcaa
     7261 ttattccgac aaagacgatt ccgactcctg cacgctttgc gaagctttcc aaattacagc
     7321 aagcaatcta tttgcaagtt ttgcgcacta ctgtggctat gtttgctgca gattatatct
     7381 acgaagagac gaccgtaatg acgggcgtac agcaattaaa gctaaaaagc atcggaaaga
     7441 tcccattaca gcaaggttgg caggaaatct tacgcatcaa gtcaaaaaag aaagaagctc
     7501 agactcttcc tcctatcgta gaaaatgaag aagcagaagt agaactgaaa agtatggaga
     7561 aagaaacaca acctccaaaa ccctataccg aaggaacgtt gatcacagca atgaaaactg
     7621 cgggaaaaac gctggataat gatgaagcgc aagaaattct caaagaagta gaaggaatag
     7681 ggacagaagc tacacgagcc aatatcattg aaaatcttaa gcagaagcat tacatcgaag
     7741 tcaataaaaa cgaaataact gttacgccaa aaggaatcac tctttgtaag gcagtagcca
     7801 atgaaccatt actgacgagc gcagaaatga ctgcgcgctg ggaagggtac ttgagaaaaa
     7861 ttggtaaaca agaggggaca ccatctgttt ttttagagaa tatcaaaaaa ttcatcctac
     7921 atttattggc agaagttcct aatcaaatcg acaaggtaga cttttcagat gagattagcg
     7981 ggacacgtca gataaaagcg atggagaaga aaaacgatca attaggaaga tgtccaaaat
     8041 gtaaaaaggg aatagtcatg ctttatccta aaatcgctac atgcttgaat ccggaatgcg
     8101 acttcaaatt gtggccaaca gtagctaaaa agaagctgac taaaacgaat ctcagagatt
     8161 tgctttcaaa aggaaagaca agtaaagttg tcaaaggttt tactggtaaa aaagggaaat
     8221 tcgatgcagt tttagtatta aaagaagata tgacaatcgg tttttctttt ccatggatcg
     8281 aaaacagtgg aacaaaagca gttgatggaa acaaagaaac atcaagaaaa aataatgaaa
     8341 acgaagtaga gtaaatgaaa acaatgaaat ag
//
LOCUS       DANKKG010000116         8241 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000116 DANKKG010000000
VERSION     DANKKG010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8241
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.116"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(93..1199)
                     /locus_tag="PW004_002245"
     CDS             complement(93..1199)
                     /locus_tag="PW004_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HDL2727198.1"
                     /translation="MIRILQCVNDMHRAGLETMLMNYYRNIDRTKIQFDFLTHRPYKS
                     DYDEEIISLGGKVYYAPRLYPQNYPKYFKWMEQFFKDHPEYEIVHSHIDAMSYLPLKA
                     AKKAGIPIRIAHSHNTSIDRDFKYLLKQYYRCKLTSVLTHEFSCGKVAGDFLFRNDKF
                     TLIPNAVDAKKFYYDESIRNDVRMKLGISSQTFVMGHIGRISYSKNHRFLIEIFKEFH
                     AIKNNSVLLIIGTGDMEEEIKNYAKKSGIDDDIKFLGNRNDIEKFYQAFDVLMLPSLF
                     EGVPLVGIEAQFADLPCFFSEKVPTEVAFSDKTNFISLNQSAKEWAREIANVDISHRD
                     SERSILIKADYNIQTAYKILESKYYELFELIQRG"
     gene            complement(1199..2335)
                     /locus_tag="PW004_002246"
     CDS             complement(1199..2335)
                     /locus_tag="PW004_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDL2727199.1"
                     /translation="MARKKLKICMLGHKRIPSREGGIEIVVEELATRMVALGHEVTCY
                     NRSGHHVSGKEFDQHKNKEYKGVKLKTIFTLNIKGIAAMSSSVFGGIRAAFGKYDIVH
                     FHAEGPCAMLWLPKLFGKRCVATIHGLDHQREKWNKLASTYIMLGEKCAVKFADEIIV
                     LSESVQNYFEDIYGRKTRFIPNGVKKIEIKSAGLITEKYGLTKDSYILFLGRLVPEKG
                     IRYLIEAFKDVQTDKKLIIAGGSSDTDEFANELKELAKGDERIIFTGFVQGQELEELY
                     SNAYIYTLPSDLEGMPLSLLEAMSYGNCCIVSNISECTEVVEDKAMIFKKSDVSDLKI
                     RLEEACNQSEMVKVLKNQATEFICSKYNWDKIVQETLNLYRGNI"
     gene            complement(2367..3818)
                     /locus_tag="PW004_002247"
     CDS             complement(2367..3818)
                     /locus_tag="PW004_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HDL2727200.1"
                     /translation="MYRKNSSGWSKHIDFILLDLFCVQLAFYISYVMRQGDWNPYVIP
                     IYRHMAIFIELENLTIIFLFEAYKNVLKRGYYKEAVASIKQSFMLLLVCSLYLITMQD
                     GNDYSRVSLFIMGVVYGLLTYIIRILWKGLLHHRMKNGEDVSLVIVTSQKIAKNVVRN
                     LKEKNYGMYKLSGVIVVDRDMYGTTIGGVPVVANADNAAEYLLQNWVDEVFINVDESV
                     PYPKELIARCSEMGLAVHMNLTKVSDSTRGKQFIGRVGDYTVITSSINVMTMRQAFIK
                     RTIDILAGIIGCIATGVIFIFVAPVIYIKSPGPIFFAQERIGQNGKTFKMYKFRSMYM
                     DAEERKAELMKENKMSNNLMFKMDFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FYNVLMGKMSLVGFRPCLKSEYEKYDFHHRARIAMKPGITGMWQVSGRSDITDFEEVV
                     KLDAEYIRNWSMGLDFRILFRTVKTVLHRNGSM"
     gene            complement(3862..4626)
                     /locus_tag="PW004_002248"
     CDS             complement(3862..4626)
                     /locus_tag="PW004_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HDL2727201.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKIPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFG
                     KEKVVQMKQVAKDLINGDEINYPIYEEVKKKKFGLF"
     gene            complement(4654..5352)
                     /locus_tag="PW004_002249"
     CDS             complement(4654..5352)
                     /locus_tag="PW004_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HDL2727202.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(5364..6143)
                     /locus_tag="PW004_002250"
     CDS             complement(5364..6143)
                     /locus_tag="PW004_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HDL2727203.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAQTQPKATNDKTAPSRRSRSRV"
     gene            complement(6159..7103)
                     /locus_tag="PW004_002251"
     CDS             complement(6159..7103)
                     /locus_tag="PW004_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDL2727204.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(7149..7928)
                     /locus_tag="PW004_002252"
     CDS             complement(7149..7928)
                     /locus_tag="PW004_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727205.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADIQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIEQIRNEKISAAYATKAQTVAEQMGYSNISY"
BASE COUNT         2340 a         1718 c         1239 g         2944 t
ORIGIN      
        1 tgttatcttt gcaattcgtc ttgttgcatt caattcttcg gatatgcgat gtataattct
       61 ttttatactt gaatgtttct gtgtcatatt ctttaacctc tttgtattaa ttcaaataac
      121 tcataatatt ttgattctaa gattttgtat gctgtttgaa tattataatc tgcctttatt
      181 aaaatcgaac gttctgaatc tcgatgagag atatctacat ttgcaatctc tctcgcccat
      241 tcttttgccg attgatttaa acttatgaaa ttggtcttat cactgaaagc aacttctgta
      301 ggaactttct ccgaaaagaa acacggtaaa tctgcaaatt gagcttcaat acccactaac
      361 ggaacccctt caaataatga tggcaacatt aatacgtcaa atgcttgata aaatttttct
      421 atatcatttc tatttcctaa aaatttaata tcatcatcta tgccactttt ctttgcataa
      481 ttttttattt cttcttccat atcaccagtt ccaataatta ataatacgga attattctta
      541 atagcgtgaa attctttaaa aatttcaatc aaaaaacgat gatttttaga atatgaaatt
      601 cttccaatat gtcccattac aaacgtctga cttgaaattc ctaatttcat tcgtacatca
      661 tttctaatac tttcatcata ataaaatttt ttagcatcca ctgcatttgg aatcaaagtg
      721 aatttatcat ttcgaaacaa aaaatcacct gctacttttc cacatgaaaa ttcatgtgtc
      781 aaaacagacg ttaacttaca tcgataatat tgctttaaaa gatatttaaa atctctatct
      841 atcgatgtat tatggctatg tgctatccgt atcggaatcc cagctttttt tgcagctttt
      901 aaaggtaaat aactcatcgc atcaatatga gaatgaacaa tctcatattc tggatgatcc
      961 ttaaaaaatt gttccatcca tttaaaatat ttaggataat tttgtggata aagtctcggt
     1021 gcataataga cctttcctcc aagacttatt atttcctcat cataatcact tttatatgga
     1081 cgatgtgtca aaaaatcaaa ctgaatttta gttcgatcta tattcctata atagttcatt
     1141 agcatcgtct ccaaccctgc acgatgcata tcattcacac actgtaatat tcgaatcatt
     1201 atatatttcc cctatacaaa tttaatgttt cttgtacgat tttatcccaa ttatatttac
     1261 tacaaataaa ctcagtagct tgattcttca aaactttaac catttccgat tgattgcatg
     1321 cctcttctaa tcttatcttt aaatcactta catccgattt cttaaaaatc atagccttgt
     1381 cttctactac ttccgtacac tctgagatat ttgaaactat acaacagttc ccataactca
     1441 ttgcttctaa caaacttaat ggcattcctt ctaaatcact tggaagtgta tagatatacg
     1501 cattactata taattcttct aattcctgtc cttgtacaaa tccagtaaat ataattcgct
     1561 catcaccttt tgctaattcc tttagttcat ttgcaaattc atctgtatca gagcttcctc
     1621 cggctattat aagtttttta tctgtctgaa catctttaaa tgcctcaatt agataccgaa
     1681 ttcctttttc gggaaccaat cgccccaaaa acaaaatata agaatctttt gttaatccat
     1741 acttttctgt aatcaatcct gctgatttta tttcaatttt tttaacgcca tttggtataa
     1801 accttgtttt tcttccatat atatcttcaa aataattttg gacgctttca ctaagtacaa
     1861 ttatttcatc tgcaaatttt acagcacact tttcccctaa catgatgtat gtactagcaa
     1921 gcttgttcca tttttctctt tgatgatcta gaccatgaat tgttgcaaca catcttttcc
     1981 caaaaagttt tggcaaccac aacatagcac atggtccttc tgcatgaaaa tgaacaatat
     2041 catattttcc gaatgctgct ctaattccac caaatactga agaactcatt gctgcaattc
     2101 cttttatatt taatgtaaaa atagtcttta attttacacc tttatattct ttatttttat
     2161 gctggtcaaa ttcctttccg ctaacatgat gaccacttcg attatagcaa gtcacttcat
     2221 gcccaagtgc aaccattcgc gttgccaatt cttctaccac tatctcaatt ccaccttctc
     2281 ttgatggaat tcttttatga cctagcatac agatttttaa tttttttctt gccatcacgt
     2341 tttactcctt ctatagtatc tttatcttac atggaaccgt ttctatgaag cacagttttg
     2401 actgtcctaa acaaaattct aaaatctaat cccatactcc aattcctgat atattctgca
     2461 tcaagtttta caacctcttc gaagtctgtg atatcacttc taccactcac ctgccacatt
     2521 ccagtaatcc caggtttcat cgcaattctc gcacgatgat gaaaatcata cttctcatat
     2581 tctgacttca agcaaggtct aaaccccacc aggctcattt ttcccattag gacattataa
     2641 aattgtggaa actcatccaa tgatgtagat cgaataaatt gtccaattcc tgtcttcttt
     2701 gttccatcag gaagtatttt attgccaatg attcttgggt caaaatccat cttaaacatg
     2761 agattattac tcatcttatt ttctttcatc aactctgctt tgcgttcttc tgcatccata
     2821 tacatactac ggaacttata catcttaaat gtcttaccat tttgaccaat acgttcttgt
     2881 gcaaagaaaa tcggccctgg tgatttgatg taaataactg gtgctacgaa gataaaaata
     2941 acacctgttg caatacatcc aataattcca gcaagaatgt caatcgttct ctttataaat
     3001 gcttgcctca tcgtcataac attaatacta gatgtaataa cagtataatc accaactcgg
     3061 ccaataaatt gttttccacg tgtactatca gacacttttg taagattcat atgtacagca
     3121 agtcccattt ccgaacatct ggcgattaat tctttgggat atggaacact ttcgtcaaca
     3181 ttgatgaata cttcatctac ccaattctgt aaaagatatt ccgctgcatt atcagcattt
     3241 gcaactactg ggacacctcc gatggtagtt ccatacatat ctctgtctac tacaatcaca
     3301 ccactaagtt tatacatgcc ataattcttc tctttaagat tacgaactac attttttgca
     3361 atcttttgag atgttacaat aactaacgat acatcttcac cgtttttcat gcggtgatgc
     3421 aacaaccctt tccaaagtat acgaattata tatgttaata atccatacac cacccccatt
     3481 ataaataaag aaactctgga ataatcattt ccatcctgca ttgtaataag gtataaactg
     3541 cacacaagta acagcataaa agattgcttt atagaagcta cagcctcttt ataatatcct
     3601 ctttttaaaa catttttata tgcctcaaat aaaaatataa tcgtaagatt ctcaagttct
     3661 ataaaaattg ccatgtggcg ataaatagga atcacatacg gattccagtc accctgtcgc
     3721 attacatatg atatataaaa agcaagctgg acacaaaata agtctaataa aataaaatca
     3781 atatgtttag accaaccact tgagtttttt ctatacatct ctcactctct cctcatttcc
     3841 tttttatcta cgaataaaga actaaaataa accaaacttc ttctttttta cctcttcata
     3901 gattggataa ttgatctcat ccccgttgat cagatccttt gcgacttgtt tcatctgtac
     3961 tactttttcc ttaccaaact ccttggcaat ctgctcatag cactctttta agtaaaaggt
     4021 gcgcttagag accccgtgtg catcagaagc agccatctgc acgagattat gtttgaccat
     4081 tgttttggct gttttctgga tatctttgcc aaatacacca acataacttg gcgcagtaag
     4141 ttgggccaaa catcccatat ctaggaacgg aagcaaatga tttgggtctt ctcgaaaatg
     4201 cgcgttgcgt tctggatgga cgatcactgg tatctttcct agttctaaca actcaaaaaa
     4261 aagccgttct gcatataacg gcacttccag tgtcggaaat tcgatcagaa gatatgtatc
     4321 atctaaatct gtaaataaaa tttcatctcg gtggatgtct tcgatgagcg ttccggtaat
     4381 acgtacttct tgtccttcca ataacgtcaa aggcaactgt cttttctcta attcggcttg
     4441 taacgaagcg actagagaaa tgacttgtga tttttcattg ctgtattttc cattattgtg
     4501 gtgtggtgtg cataaaatat gcgtaatgcc ttgctgaatc gctttttcag ccattgcaat
     4561 acttgcttct aagttttctg ccccgtcatc gattcctggt aggatatggc aatgtagatc
     4621 gatcatttct cttgtctcct ctcttcttat tccttaattt ccgtagtaat aaccagcatc
     4681 tttgctgtgt tctgctccgt tgtatacgac acctaagact cgtgcttgta ccatatctaa
     4741 cagttctttt gctttagtca atgattcttt tcgtgacgta ttttcccgta cgaccaataa
     4801 ggtgccatcc gtttttgaag acatgatctg tgcatctgtt acggcaacga ctggcggcat
     4861 gtcgaagatc actacatcaa atagttgtct tgcttctgct aaaatctgat ccattcttgg
     4921 cgaacttagt agttctgatg gatttggtgg tttaggtcca cttggcagaa tcgacaaatt
     4981 gtcgactggt gttcgttgga tctcatctgc tacacttcct gatgaactaa gaacagtact
     5041 taatccgctt gcgttgttca acttgaacgt tttatagatg acgggcttac gtaagtctgc
     5101 atcgactagc agcacccgtt gaccagattt ggcaaaaaca acggcaatgt ttgctgcagt
     5161 ggtcgatttc ccttctccag gtcctgatga agtcacaaca atcgttttga tttgctgacc
     5221 agctgcggag gcaaactgga tattcgtccg aatcgtacga tattgttcag cgattggtga
     5281 agagggattg gtgagtgtga ccaaactgac tgcatgagtt tgggctgctt tttgtttctg
     5341 tgttcgtgcc attttctttc ctcctagact cttgagcggc ttctgcgtga tggcgctgtt
     5401 ttatcgtttg tagcttttgg ttgtgtctga gctggtcctt ttttacggat accagcattc
     5461 aattctttcg ctgacatttc tggaactgtt cctaagattg ggaatcctaa tgtatctgtc
     5521 acatattttt catctttcac tgtccgatcg agtaattcca ataagaatgc cagacctaca
     5581 cctaccatca ttcctaacat taagccagtc attaaattca atttattatt tggcgaaaca
     5641 ggtgtcgtat tggcaactgc acctgaaatg atcgagattt tatcaacatt catcacatct
     5701 tttgcgttct cttggaaaac ttgtgaagtt gtgttagcga tctgttgagc agccactgca
     5761 ttcgtactta ttgcctgaat cgagaacatt tgtgaatttt ctgcctgatt aactgagatg
     5821 gcatctttga tttgttcagc agtcattttc acattgtctt ctgtttctaa acgatctttg
     5881 acttcattga gcactaaatc actgacgatc atatctttat acgtattgat catttgcagg
     5941 ttcgtattga catcattgac atttgcagtg tctgattgtg gcagtgtcac gatcaattgt
     6001 gcttgtgaac tgtattttgg tgtcaagatg aaaaatgtta cgattcctga gatggccaag
     6061 ccagcaaaca ttgagatgat gattattacc aagtgtttgc gtaatgtttc aaaaatttct
     6121 ttcaagctga ttgtctcttc cattttttct ccactttcct atttattctc tagattcatt
     6181 tgatctttta attctttttt cacacgatca agttcttgat cactgacttt ttgataggag
     6241 atattatctt gcatgaagcc ttctccttgc agctgatctt gcttaatcgt accaaatgcc
     6301 tcccgataat ctaaagcaat ttttttcatt tcatcaaagt tcatgtctgt tttcatattc
     6361 gactctagag cattcaaaac ggattgatag ttgctgacac cgtttagact caatactttt
     6421 ttcgctacgc cttctacgat ctttctttgg cgcccttgtc taccgtaatc accatttggg
     6481 tcttcataac gcatacgaga ataagataat gcctgatccc cgttcaatgt aattttaccg
     6541 attggaaagt catagccatc ttgtgagaaa gtgaggtcat tattgacttc gataccacca
     6601 actgcatcaa cgagttcctt tagccctttc atattgatag agacatagtg atcgattggg
     6661 atgtctacat atttttgtac ggtatccatc gccatggcag ctccgccgaa tgcgtaagcg
     6721 tggttgatct tgtcttttgt gtcacgacct acgatgtcga catacgtatc acgaggaatg
     6781 ctgacgatcg ttgtctgttt gtcttcagga ctgactgtgg caaccatcat cgtatctgat
     6841 cttccttgat cgacccgacc taaatctcca gtatcaatcc ccatcaatag aacagagaaa
     6901 gattcttgtt tgtccagatc aactggtttc gttcgttctc cttgggttcg ttccactgat
     6961 tcgtaagttt tctcaataga atgtgagaga tccagatata attttgctcc tactcctaca
     7021 attgctaata ctaatacgat aagaactgct aaaataatca gaaatatttt tttccctctt
     7081 gattttcttc ttcgtcttgc catagtttcc tccgtttgct aaccttgctt gaatcaacgt
     7141 tttaactgtc aatagcttat attgctatag cccatttgct cagcaacagt ttgtgctttt
     7201 gttgcgtatg ctgcgcttat tttttcattc cggatctgct ctatttgact gagcaccgtc
     7261 aaatattgat cgtaagtagc ggtggcagtc acttgaccat cctttaacat cgtatcgaat
     7321 aactgatcta gttgcgtgac ttgcgcgatc tgaatatcag cgaatcctag atattgcata
     7381 acaaccgttt tccaaggact atctttaaaa aagctcatat tttcacgtac acgagcgaca
     7441 tctgcacttg tcaccttttc tttgatgatg acatcgtcta cagcttgctg ccagtttgat
     7501 acatttttag taaataaatc gttgacagct gtctggatct tcagttttga ttctgcacta
     7561 tctaactgtg tcacgattgc tttcttctca tcttggattg caacagcttc tttaggcaat
     7621 tctgattctt tgatgtcgta atcatctgct gaaagtttga cagaggatag cttatttgac
     7681 aaacttgtga ttgtttcttc tgttatttca gggtttagaa aaattttttc ttttgtacta
     7741 tacagcttgt ctacttcttt tgataaagaa gtcaaggtgt ctttttgatt ggtgatggca
     7801 gattctgcat aattgatccg ttgctgatgt tgccgctgat tattgatatg gtatccaaac
     7861 aaacagatcg caatagcacc tgcacctacc gccacagcag tcaatgactt gacgaatggc
     7921 ttttccatag attcactcct aagtattgat gatagataaa ttaagatata attcacttac
     7981 ataaaaatga cagcttttta tttaaaaaaa cataattaaa ctaatatatt cacagaaata
     8041 tttgtaaaca agttttccct atataatcat accacaaact gttttcttga ctagggcaat
     8101 ttacgattta aaagtaaata aaacattaat aagataaaaa ttaatcgatt tcatttttat
     8161 ttccataatt ttcataggtg tttttaaaag ttacacaaaa aatagaataa caaaaaaatt
     8221 aacattgaat taataatagt t
//
LOCUS       DANKKG010000117         8180 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000117 DANKKG010000000
VERSION     DANKKG010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8180
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.117"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..371
                     /locus_tag="PW004_002253"
     CDS             <1..371
                     /locus_tag="PW004_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2727206.1"
                     /translation="EAANDEFKKMSLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENI
                     MLIFGNTKEIRQFCEAYPKVQEINYGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALG
                     IKQFIQQVPTSKKEDLNTML"
     gene            385..1158
                     /locus_tag="PW004_002254"
     CDS             385..1158
                     /locus_tag="PW004_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2727207.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAAAPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            1172..1984
                     /locus_tag="PW004_002255"
     CDS             1172..1984
                     /locus_tag="PW004_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL2727208.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            2038..3003
                     /locus_tag="PW004_002256"
     CDS             2038..3003
                     /locus_tag="PW004_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL2727209.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            3119..5842
                     /locus_tag="PW004_002257"
     CDS             3119..5842
                     /locus_tag="PW004_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL2727210.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESKILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGEAIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            5855..6487
                     /locus_tag="PW004_002258"
     CDS             5855..6487
                     /locus_tag="PW004_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL2727211.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKDLRKEVKPLLDFLKKRDIP
                     LALASSSPVVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTVALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            7386..>8180
                     /locus_tag="PW004_002259"
     CDS             7386..>8180
                     /locus_tag="PW004_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2727212.1"
                     /translation="MKNLQLKFIVDPSIVRWFRIINQFERNQLATKGELAKSNHVSER
                     TIQTDVNKMKDYFSESVQFVSTKSGYSFQKERPVLFLKQKEQLLENEILFELLGNVFY
                     GELEDMTELIDRFHLSEATFRRYLKQIQPVLEEYGLELSLYPLDLKGEEANIRKFFKD
                     FYYEGEMTPHTLVPPRDLHELVQATFYDKFEYFSIGTGTSPAEFYYSLYIAIERVRQG
                     KTIAIPQGLIERVIASENFRLMYSLKEAIQTNYQVALSEEEFCWLYL"
BASE COUNT         2767 a         1244 c         1613 g         2556 t
ORIGIN      
        1 aagaggcagc gaatgatgaa ttcaaaaaaa tgtctttgaa tttatctaaa ccgactggca
       61 ttaaattaac aattttttca gttgaacagt tactagaaaa aatgcctaaa atcgaaacac
      121 ttaacgaaaa tatcatgctt atttttggta atacaaaaga aattcgtcaa ttttgtgaag
      181 cctatcctaa agtacaagaa atcaactatg gagggataat caaaaaagaa ggttccaagc
      241 aatttagtaa tgccattttc ttgaatgaat ctgagcaaga agatgccaaa aaattaaaag
      301 ctcttggcat taaacaattt attcaacagg taccaacttc taaaaaagaa gatttgaata
      361 caatgttata aaggaggaat gaaaatgctc gttcagtcaa ttggtttagg gttaatcggt
      421 gttttttgta ttttagactc aaggctttta ggaagaatga attttgaacg accgttgatt
      481 gtcagtacgc ttgttggttt ggtacttgga gacatggaaa aaggattaat tgttggagcc
      541 tctttggaat tgatgtcctt aggattagta aatatcggag ctgctgcacc acctgatatg
      601 aatatggcat caattattgc tacagccttt gccattttat caaatgcgaa tgcagaagca
      661 gcattaacga ttgccattcc aatttcagtg ttaggtcaaa tactaggtat cttaatgcgg
      721 acgattttgt caaatttgac tcacgttgcg gatcgcttta tcgaacaagg gaaatttagc
      781 caagcaagga gaattcatat actttatggc tcgggattat atgctctcat gtattttatt
      841 cctatttttc tagcaattta ttttggaacg aatcttgttt ctcagattgt tgatgcgatt
      901 cctgagtggc taacaaatgg cttaacggtt gcaagtaaaa ttttaccagc atatgggttt
      961 gcgttgttat tacaaacgat gttaacgaaa aaaatggtgc catttttatt acttggattt
     1021 ttaattactg cttattctgg attgggaatc attggtattg cagccttcgc atgtattgtt
     1081 gcgtttgtca tggttcaagt tcaaggtaaa aactcaaatg gtaaccaaaa tgagccagat
     1141 ccattagatg acttataagg aggtagatta agtgaaagag aaagatcaaa atgcatggaa
     1201 atatcggcag ttcttatggc gttcatgggc aattcaagcc tcatggaact atgaacgaca
     1261 aatgaacatg ggttttatgt acggaattgc accaattcta gataacattt acaagaatcc
     1321 agaagatgaa gcaatgaaga aagaagctta cgagcggcat atgatgtact acaactgtac
     1381 ccctcaaaca agtgcatttg tgttagggtt aactgcttct atggaggagc aatactacga
     1441 agaccaagaa aactttaatc cggaatcaat cagtgcagtg aagacttctc tgatgggacc
     1501 gctatctggt gttggtgatt ctttctttca aggaacgatt cgggtattag cctttggact
     1561 aggaattaat ttagcccaac aaggtagtat tttaggtcca attctagcaa tgttaatttc
     1621 ctttgtacct tcctttttag ttacttacta tggtgggaaa attggctata acatggggaa
     1681 taaatacttg tctaaactgt atcaagaagg tttgatggat aaagtaatgt atgtttgctc
     1741 cattgtgggg ttgatggtca ttggttcgat gattgccagc atgatcggga ttacaacacc
     1801 aattcaaatg ggtaaagctt ttgtcttaca agatgtatta gatagtatta tgccacaatt
     1861 tttaccatta ggattaacct tcttcatgta ctggttattg cagaaaaagg tcaaaacagg
     1921 atggatgttg gcaatctgtt tagtcggagg aattattttt agtttgttag gtatcttaca
     1981 ataagggaat aaccaataaa tttggttgaa taatataaat aaggaggaat tcaattgatg
     2041 aattcaaaag aaattgttaa agaaatcgta gcgaaaaaag aggtaaaatc aatcgtattt
     2101 gttggctgtg gcgcttcaaa agcggacctt tatcctgcaa agtattttct agaccaaaat
     2161 gcgaagcagt tacggattag tcattacaca gccagcgaat ttaactatgc aacaccagca
     2221 tctgtagatg agtcaactgt cgttatttca gcttctttag gaggtacgac tccagaaaca
     2281 gttgaagcta acgcagtagc taaagaaaaa ggtgctacag ttattagtct aacacgaagc
     2341 attgactcac cgttaacaaa agatgcagat tatgtaattt atcatcgatt tgcagagaac
     2401 tatgctgcta aattagaaaa aacaggctac tgtttagaat tggcagtaga acttttacat
     2461 caagtagaag gttatgacca ttatgaagca atgcaagaag gatttgctac tatctatgat
     2521 cttgcccaaa aaagtgcaga atcagctcgt aaagatgcga agaaatttgc tgaacgcttt
     2581 aaagatgaaa aagttattta tgtaatgagt agtggtgcta cacatgaagt agcatactca
     2641 acatcaattt gcttgatgat ggaaatgcaa tggattaatt ctggtacctt ccattcagga
     2701 gaatttttcc acggaccatt cgaaattgtg gataaagacg taccgtttat attattaatg
     2761 aatgatggaa aaactagaaa tatagatgct cgagcattaa ctttccttga acgctttgat
     2821 acgaaaacag aagttgtaga tgccttggat tatggcttgt cttcacatat tgctaaagaa
     2881 gtcttagact actttaaccc attcgtgatt actgcagtat ttagagttta tgcagaagaa
     2941 ttgtcagaag caagagaaca tccattaact aagagacgtt atatgtggaa actaacttat
     3001 taaaaaatag atcaaactaa ataaggtggc ttgagaaatt aagccacctt gttttcttca
     3061 atgtgatttt atacatatgg taaaataact agtacatatt gtgaaagcag gtggcaagat
     3121 gcagcgatta aatgactatc tttatcaact aacattgctt ttgaaatcta cgccttcaga
     3181 taattattca gctcgcagta tttcgcaagc tttacagttg aatagaagta caatcagtag
     3241 ctacttaaat gaaggtgttc gtgaagggct tttcataaaa gtaaaaagtt acccggtttt
     3301 atttttacat agaacagctc ttgaagaact acatatcaca ctgaataact ccgaaataga
     3361 atcaattgaa agcttgttaa caatttctca aaaacccgca ttagatcaag taattggctc
     3421 taaaggaagt ttgaaagaag caattgatca aattaaaacg gcagtcttat atcctggaaa
     3481 agggttgcct ttattattga tcggcgctag tggatcaggg aaaacctttc tagcgaataa
     3541 aatatatgaa tatgcagtag aagaaaaggt cattcacaca tcggcacctt tcatagatta
     3601 taattgtgca caatatgtga gcaatccaga gttactatcc tctgctttat ttggctacac
     3661 gaaaggagca tttaccggag caagccagga gcatactggt ttgctagaaa aagccgatgg
     3721 aggagtattg tttttagatg aagtgcatcg cttatctgaa gaagggcaag agaaattatt
     3781 tacatttatg gatacgggtg aattctctcc tatgggggat aatagtattc ggaaaaaagc
     3841 cgatgttcga ttagtctttg caacaacaga aaatatatat acgactttct taccaacttt
     3901 tcttaggaga ttgccagtta ttgttaatct acctagattt caacaacgtc cgtcgtttga
     3961 acgtctgtca ttgattgatg aattttttgt ttctgagagt aaaattttag ctaaggaact
     4021 atctgtttct gatgcattga ttcatttctt aatgaatagt gattttgaag gtaacgtggg
     4081 gaatattaaa aatattatca agtatgcttg cggaaacgca tatatccacc agaagagtca
     4141 gcaaacaatt caagttaaat tattggatat gcctttagag tacagttata aattcaagga
     4201 gcaactaaat aaacccagaa agaaaagagc agatcgaatc tactatccag aagaaacacg
     4261 acaagtgcaa ctagaaagaa acaatcagct ttttgttcaa ttttttgacc aattacttac
     4321 tggattctct gaagtgattg aaaataacaa ggatattaaa ctatttttag aagaaatggt
     4381 taccaaggta acacaagtaa tggatacgct tatttttcaa gaggattacg aaaaagagca
     4441 gtctttatac gccattctaa actatcatat tcgtcaaagt attgatttta tgcgagaaag
     4501 ctacgggttt gaacaagatg gtaaccgggt agtcgctttg gccaactatc tatatgtcaa
     4561 ggataatcaa gtgatttttg aaaatagtct tcaagaaaag aaaaccaaag atcagcttaa
     4621 cgaatttttt gctaatgttc ttgaaacccc ttattggtat gcaaaaaaaa tcttaagcct
     4681 tttatctgag tgtttagatc aacctgtgta tgaagaggac attttcttta ttacgctata
     4741 tttttatagt ttgaatgtca cgaatgttaa aacaggtatt aagagtattg tgctagctca
     4801 tggttattct acagctagta gtatggcgaa tgttgccaac cgtatgctga ataagaacgt
     4861 ttttcaagct tatgatatgc ctttagatat tacattagag aaaattgaag caaaattgat
     4921 tcaattcctt ggagattatc gaacagattc aggcttgatt ttattggtag atatgggttc
     4981 actaaaccaa ctaggcggtt tattagtgaa tcatctaaat ggaccactgt taatcattga
     5041 tcatatcaat acaccgttat tattggaagt cggccaacaa gtattaaaca ataaaagtat
     5101 caccgaaata ggagcagaaa ttgaaacaag tcttactttc aaaaagcagt tactacttcc
     5161 caagaaaaag aaaaagaaag ccatcattac ttgttgttat acagggattg gtagtgcgac
     5221 ccagattcag gagattttaa cgaaatgctt gaaagatgca gttagagaat tagtaattat
     5281 tccgtatgat tacaaaaagc tgttagaaaa caagaatttt gagactcctt ttcaattata
     5341 cgatgtcatc atgattgtag gtacagaaga tcccaaaatt aatcagattc catatattgg
     5401 attagatcgc ctgattaatg gggaagcaat tggcgaattt gttcattact tggaaaaaga
     5461 gctgactatt gaagtgaacg atttacaaaa agaattaatt ttcaattttt caattagtaa
     5521 aattgtcgaa aatttaacca ttttggatcc agataaggtg ctatcttttg tacaaaaagc
     5581 aattaaagaa ttatcgaagt tattaaattt tgatttttct aataatcagc tgtttttgtt
     5641 gtaccttcac tgtagttgta tgattgaacg aattgtacgt aaagaaagtg tggatgagca
     5701 agaggacatt gaagaatata tgcaagcaaa tggagaaata atggataaaa ttcatagcgc
     5761 tttttttgag attgaaaggg aatacacaat tgaaatacca gatttagagt tgcgattatt
     5821 gagcgatatt gttcaaggat aggagtgaat tcgaatgaca atgggagtaa tttttgatat
     5881 ggatggggta ctcatagaca gcgagacatt ttattttgag agaagaatga acttctttca
     5941 agaactggag atagatccag gatcaaacca gattgatgat tatgtgggga aaacagaagc
     6001 tgcaatatgg gaaacattag ttcctgagga caaatcatta agagaggaac tttatccgaa
     6061 atatgtagat tatcgaaaaa cgcatccaat tgatttcgct aaagatttac gaaaagaagt
     6121 gaaaccgtta cttgacttcc tcaagaagcg ggatatacca ttggcactgg catcttcttc
     6181 tccagttgtt gagatagaga gtatgctcat tcaatgtgaa cttactgaat atttttcctt
     6241 tgttataagt ggtgagtcat taaaacaaag caaaccacat cccgaaatat atttaaaagc
     6301 gaaacagcaa ttagactgtg acttctttat tgcagtagag gattcaacga ttggtattaa
     6361 atcagcaaag gcagcaaata tctatacggt ggcactaaaa caaccaatgc aaatcgatca
     6421 gagtgaagca gacatacaaa ttgaaagtct acaagaactg gaacaaattg ttttagaaaa
     6481 aggttagtga attaatggaa ataaggctat atatctgtgc tagctatttt gactaaccaa
     6541 aaaaacttgc tataattcag gtgtagtaaa aggtgctggt aacacctttt acgaaaatgc
     6601 ttcggcagtg ataatgggat tggcttttta agttttttat tttgttcgca atccctaagg
     6661 tcatgtgaat gatcctaggg attgctagaa tattttgcaa cattctatct atttcagaac
     6721 gtaaccttcc ccagttcatt gggattcatt agcatacaac caacatttca aaaagaaatt
     6781 gcactacacc attattagct attttgacta accccaaaaa acttgctata attcaggtgt
     6841 aggtaaaagg tgctggtatc accttttacg aaactgcttc ggcagtggta atgagtgtgg
     6901 cttaaaggac tagtccttta agctttttac tttgttcgca atctctaaga tcaagttaat
     6961 gatcgataag attgctagaa tattttgcaa cattctattt cagaacgtaa cctttcccat
     7021 tccactgtga ttcattggca accctcctca caaccaacgt ttcaaaagaa attgtaaagt
     7081 tgttggggta ccacgaccga acaatactac acctgaatga tagtaagttg tctcattaag
     7141 tatagcatat cctctttttg ggtaaataga aatcggttac attttttatc caagaccaag
     7201 ttttttcatt tggtcttatt tgctataccc aaacaattcc gcagtatagt cgacggtatt
     7261 gattttatca ggtgagtaat agaaacttgt tttccttccc caaaacatta cctataaagg
     7321 agaaaaaatg gtatcgtaat tatataggta taggatgaaa tttttttcta agaggagggt
     7381 tgttgatgaa aaatttacag ttaaaattta ttgttgatcc atcgattgta cgctggtttc
     7441 ggattatcaa tcaatttgag cgcaatcaac ttgccacaaa aggagaatta gcaaagagta
     7501 atcatgtatc tgaacgaacc attcaaacag atgtcaataa aatgaaggac tacttttctg
     7561 aaagtgttca atttgtttca acgaaaagtg ggtattcttt tcagaaggaa agaccggttc
     7621 tttttctcaa gcagaaggag caactactgg agaacgaaat tttatttgag ttgttaggga
     7681 atgtctttta tggcgaattg gaagatatga cggagctaat tgatcgtttt catttatctg
     7741 aagcgacttt tcgtcggtat ttgaagcaga ttcaaccagt tttggaagaa tacggcttag
     7801 aattgagtct ttatcctctt gatttaaaag gagaagaggc aaatattcgc aagtttttta
     7861 aagacttcta ttatgaaggg gagatgacgc cccatactct tgttccacca agggatcttc
     7921 atgaacttgt gcaagctaca ttctatgata aatttgagta tttctcgatt ggaacaggga
     7981 cttctcctgc tgaattttat tatagtttgt atattgcgat tgagcgtgtg agacaaggaa
     8041 aaacgatagc cattcctcaa ggtctgattg agcgagtaat tgctagtgag aattttcgct
     8101 taatgtattc cttgaaagaa gcgattcaaa caaattatca agttgcttta tcagaagagg
     8161 agttttgttg gttgtattta
//
LOCUS       DANKKG010000118         7849 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000118 DANKKG010000000
VERSION     DANKKG010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7849
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.118"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            933..2321
                     /gene="ltrA"
                     /locus_tag="PW004_002260"
     CDS             933..2321
                     /gene="ltrA"
                     /locus_tag="PW004_002260"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HDL2727213.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
     gene            complement(2443..3738)
                     /gene="purB"
                     /locus_tag="PW004_002261"
     CDS             complement(2443..3738)
                     /gene="purB"
                     /locus_tag="PW004_002261"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HDL2727214.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(3751..4875)
                     /gene="purK"
                     /locus_tag="PW004_002262"
     CDS             complement(3751..4875)
                     /gene="purK"
                     /locus_tag="PW004_002262"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HDL2727215.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(4868..5356)
                     /gene="purE"
                     /locus_tag="PW004_002263"
     CDS             complement(4868..5356)
                     /gene="purE"
                     /locus_tag="PW004_002263"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HDL2727216.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(5772..7079)
                     /locus_tag="PW004_002264"
     CDS             complement(5772..7079)
                     /locus_tag="PW004_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HDL2727217.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(7082..7663)
                     /locus_tag="PW004_002265"
     CDS             complement(7082..7663)
                     /locus_tag="PW004_002265"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDL2727218.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(7779..>7849)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
BASE COUNT         2352 a         1720 c         1481 g         2296 t
ORIGIN      
        1 atttcatttt ttaaaaaagc taaaaacctt gatattactt cttttcaggc aatacgaata
       61 ttattcgctt acattttttt gtataaattt agttgtttta aatttaattg tctgctacgc
      121 ttgtgatgaa ttaattttcg ttctgcactt ttggacgata aatttggaca attgaaaata
      181 caaaaaaaag aaaggccctg tgagactcgg aaggctggta accttcgtcg acgtccacag
      241 gtggcattaa gacaaccttt aatcccttaa tgcgtttacc tttcagtgtt acggtattac
      301 ccgcaacgga tttagtataa cagatattca aaagtgatac cacttttttt ggctgtgtta
      361 aggttaaaaa tacgtgatat tacgatgaat ctatgcctaa gcataatgtt ataaaaatgt
      421 ccgtgtggct ctttgccgta tgacgaacgc tgaaattaga gggaacgcat gtttggatgc
      481 gttccctctt ttttgttgtg cgccctgcat gggtgaaaac ttggtggtga aagtccacta
      541 caggcttggc agtaggaact gttagcgaaa gacaaggcgg caatcgtgag gttgtggctg
      601 aaggaagtcg gacgcaaact cctgaactga cgaacagaaa ctagatacaa ggctgaatta
      661 ggtcggataa gtcttcatca caagacaaag tccaatactg cccgaaacct atacagtaaa
      721 tctagcagtt acatgggagg aaagttttca ctcttaccgg gggagatctc atgagggtgt
      781 tgtgggcgtt gaatgaaaac aaaccagcac aacgatcatg gcgacatgat ggtgaatcat
      841 gagaagtcag ccgaggtcat agtagtaacg acactgaccg ttacgaagga ctgaacaata
      901 agaatcttga ataatttagg aggtgtgaga agatgcgacg accgcagaaa acaagaaaac
      961 ttggctatca tcaaagagct agtgtggaac acgaagggta tgatgaagtg cgcagcactt
     1021 cttgcggtga acagaggaaa caagatggtg tagaaaatct gtttgggaga attctagatc
     1081 ggaataatct caatcaagcg tatcttcaag tagtcagaaa taaaggggcg gctggtgcag
     1141 atgggatgac cgtcgatcaa ctgcttcctt acttgaaaga gcaccgcgag gaattactat
     1201 ccaatttacg ccaagggaac tataaacctc agcctgtttt gcgagtagaa ataccgaaac
     1261 caaacggcgg tgtaagaaaa ctaggaattc ccacggttat tgatcgtatg cttcaacagg
     1321 caatcaatca agtgttacaa ccgctatttg aaccagaatt ctcagataat agttatgggt
     1381 ttcgcccaaa tcgcagcgca catgacgcag tgaagaaagc aaaatcttat tatgaacaag
     1441 gttaccgata tgtggtggac attgatatga aagcctactt tgacaccgta aatcacgata
     1501 agctgatgta tttcattgag cggaaagtaa aggacaaaca agtcctaaaa ctgatccgtc
     1561 aatacttaat gagtggtgta atggaaaatg gactcatcac aacaactgaa gaaggaacgc
     1621 cccaaggagg caatctctct cccctgttaa gtaacatcta cctgaacgaa tttgatcaag
     1681 tgctagaaaa tctaggacat aaatttgttc gatacgccga tgactgcaat atctatgtta
     1741 gaagcaaacg agcaggacag cgtgtgatga agaaatccat tcaatttctc gaagaacaac
     1801 taaagttgac ggtaaatcga gaaaagagtg cggtaggaag tccattgaaa cgaaagtttc
     1861 ttggattctg cattctgcca acaaagaaag gtattaaaat tcgccctcac gcaaaagcaa
     1921 aacagcgtgt gaaaagcaag ctcaaacaac tcactaaaag aaaccgtgga agaagtatcc
     1981 aacttatttt aaaagaaatc aagcaactaa tgactggttg gatcaactac tatggaattg
     2041 gtgagatgaa agaattcatg agggagttaa acggttggtt gaagcgaaga atcagacaat
     2101 atatttggaa acaatggaag aatccaagaa ccaaaagaaa aaatctgatt cgtttaggga
     2161 tcgataaagc gaaagcgtat gaatggtcaa atacaagaaa aggtatttgg tcagtctcca
     2221 aaagtcatat tcttcatcga tcattaacag acaaagaact ggcccgccaa ggatatgagg
     2281 acatctcctt gaagtaccag ttcgtacact caacttattg aaccgccgta tacgggtccg
     2341 tacgtacggt ggtgtgagag gggcggaagg tcagttggct ttcccctcta ctcgattatt
     2401 tttgctattc tattttaatt gaattttgca actcactctt ctttattcca aacctactcg
     2461 tttgaatatc acgtcaacgt ttttcaaatg atgatgataa tcaaaggcat cgtctatttc
     2521 ttctttagat aataccgatg tgatggttgg atcttcttct aacagcgggc ggaactgcac
     2581 ttggttgtcc caagcgtacg cagtttttgg ctgaacaaga tcataagctt cttctcttgt
     2641 cattccctta tcgatcaatt tcagcataac gcgctggcta taaatcaaac caaatgttgc
     2701 agacatattc cgcttcatgt tttcaggaaa gaccgttaga tttttaacga tattgccaaa
     2761 acgatttaac atgtaattca ataaaatcgt ggtatcaggc aagatgattc gttctgctga
     2821 agaatgagaa atatctcttt catgccataa tgtgacattt tcataggcag tgaccatgtg
     2881 tccgcgcacc actctggcta gaccagccat attttctgaa ccgatcggat tccttttgtg
     2941 cggcatagca gaagaacctt tttgaccttt cgcaaaaaat tcttctactt cacgtgtttc
     3001 ggatttttgc aatccgcgta tctctgttgc aaaacgttca atacttgtag caataagcgc
     3061 catcgctgag aaatattcag catgtagatc ccgagggaga acttgagtag agatttcttg
     3121 tggacggatc cccaattgtt tacatacata ctcttcaata aatggcggga tatttgcgaa
     3181 cgttcccact gctcctgaga tttttcctgc ttctactcct tttgccgcat gttcaaatcg
     3241 ttcgatgttc cgtttcattt ctgaatacca tgtggcaagt ttcaatccaa aagtagttgg
     3301 ttcagcatgg acaccgtgtg tccgacccat catcactgta tatttgtgtt cttttgcttt
     3361 ttcgccaatg atttccgtaa aacgtttcaa gtcttcccgt aaaatatcat ttgcttgctt
     3421 gatgagatag ccataagcag tatctacgac atccgtactt gttagaccat aatggaccca
     3481 ttttctttct tctcccaatg tttcagaaac cgctcgtgta aacgcaacga catcatggcg
     3541 tgttaatttt tcgatttcca agatccgatc gatatcaaat gatgcatgct cacgaatttt
     3601 tttcacatct tcttgcggga tttcaccaag ttctgcccaa gcttcatctg ctaaaatctc
     3661 gacttcgagc cacgcacggt atttgttctc ttccgtccag atattcccca tttcagggcg
     3721 tgtataacgt tctaacatgt tttttctcct ctaatcccaa atattcgttt gtttgatttc
     3781 ttccagcgtt tgctggatgt cttctgtcaa gatagtgata tgtcccatct tgcgaccttt
     3841 ttttgcttgt ttttttccat agaaatggaa ttgccagtct ggcttattct caatcaagcg
     3901 atagctttcc agcagttgtt ctcccaaaac attgaccatt actgcattgc ttatcaaacg
     3961 tactttttct cctaatggcc aaccacaaat acctcgaata tgggcatcga actgactcat
     4021 agaacaagct tcaatagaat aatgtccaga attgtgagga cgaggagcta attcattcac
     4081 atatagcccg cctgtttttg ttaaaaacat ttctacccct aaaacacctg aaaggctcac
     4141 tgcttctgca atcacacggg caatacgctc agcctcctcg atcacatcct gatcaatcgc
     4201 ggctggagca atcgtttcat gaagaatatt gttgtggtgg atgttttcca cgaccggaaa
     4261 agtcgtgaaa tctccttgac catttcctgc caccatgatc gaaatctctt tttcaaacgg
     4321 gatccatgct tctaaaacac aggttccttc tcttaaaagg tccatggcag gagctagatc
     4381 cgcccgactt ttcaaaacat actgcccctt gccatcatag ccgcctcgag tggttttcaa
     4441 aacacaagga tagccgatcc cgtcaatcgc atcttggata tccgtcggac tgacgatcgt
     4501 tgcataagga gcaatcacga tgttattcgt ttccaaaaaa gatttttcta acaggcggtc
     4561 ttgcgtaatc gcaagcaagt ctgtcccttg aggaataaat gacatgggta aaatcgcatt
     4621 taacgcctct acactgacgt tttcgaattc ataggtaatg acatctgttc ttctggcaag
     4681 ctctttcagc gcaaaggtgt catcatagac actttcaatg tgccaatctg caacttgtgc
     4741 ggctggacaa tcgctcacag gatccagcac acctacttta aagcccattt cttttgcact
     4801 aatcgccatc atacgaccta attgaccgcc accgacgatc ccgatcgttg aaccaggcaa
     4861 caaaggttta accaagttga tcactacttt ctataacagt tttagccaac attgaacgtc
     4921 tttcagccaa tttagcctcc aattctaaat catacatgga aagcatctgt atcgcaagta
     4981 gtccagcatt aattgctccc gcttttccga ttgctgtagt agctacaggt actcctccag
     5041 gcatttggac aatcgaaaga agcgaatcca aaccatttaa tgttcgtgat tggaccggta
     5101 caccgataac cggcaaagtt gtttttgatg cgaccatacc cggcagatgc gcggctcccc
     5161 ctgctcctgc aataatgatt ttgattcctt tttttcgagc gttttgtgca tattcaaaca
     5221 tcaaatctgg ggttcgatga gcagaaacga cctttttttc ataagacaca ccgaattcat
     5281 ccaatatttg acatgcttct ttcattgttt cccaatctga agtactcccc ataatcacag
     5341 agatttttgc tgtcattgtt tttcctccca gtttttctac attcatattt taacaatcac
     5401 tttttttgat gtcaaagaaa tttcgaatgt taacaaagaa aaacatttta ttgttcgtgt
     5461 ttattaaaga taaaaaaaag gaatgtgaca aaagttaggt cacattcctt tttatggata
     5521 aacggtgttc tcctgccaac tcctcggtca caaggaacaa tattcaacaa acatgagaag
     5581 cttttttttg aaattgttcc ttgtgactcg gagatgaacg gcaggctcac gacctcttcc
     5641 ttcaacggtg ttcaatcagc tgaccctcgg tattagctca aacaaacggc aaaatacgag
     5701 gaactatttt tccgtttgtt tctctaacaa tcaggtcaaa acgctgattt cacaaccttt
     5761 cttttgctct attacttctt gaaggcttct tccaatattt cttcatcttt tttcaatcca
     5821 ttaaagatca aattcaatat gatagccgta atgctactca ttacgatgcc atttccagtg
     5881 aacatttgca atgtttgcgg catttgactg aataatgtcg gcgtaacatt gaaacccagc
     5941 ccaaatccaa cagaaacagc aacaatcaat agatttttat cattgctgaa atcaacttcc
     6001 aacagcatac ggattccttg gactgccacc atcccgaaca tgactaacat tcctccacca
     6061 agtaccggct cagggatgat ttgtgctaac gcaccgattt tagggaataa tcctaaaaca
     6121 atcaagaaga atgcagagaa atagatgggt tttcttgttt tgattcccga aagttgaacc
     6181 aatccgacat tttgagaaaa gccagtataa gggaatgtat tgaaaatacc gcctaagata
     6241 acggcaagtc cttctgcacg atatcccttt ttcaattctt cttctccaac atttttccca
     6301 gtaatgtcac caagtgcaaa atatacacct gttgattcta ccatgctgac catggaaatg
     6361 atgatcatta gtaaaatcga ccatacgtcg aaagttggtt taccaaaata aaagggttgt
     6421 ggaacatgga acaacggcgc atctccaatc gcgctcagat caatcattcc catgaaagca
     6481 gctaagatgc ttcctccgac taaaccaatc aataccgcga ttgaacgaat gaaccctttt
     6541 gccagcattt gcacgcctac aattaggaga atcgtcacaa atgcaagtag cagatttgtt
     6601 ttgtcaccaa aatcagctgc tgttgcatta cctccaccca ttttctcgat tgctacagga
     6661 atcaaagtca aaccaataac ggtgataacc gttcctgtta ctataggagg gaatagtttt
     6721 ttaattttag aaaagacacc agaaactaac acaacaaaga tcccagaagc gataatagat
     6781 ccgtagatag ctcctactcc ttcattggtt ccgatcaaga tcagtggagc tactgcttgg
     6841 atcgcacatc ctaaaacgac aggcaagcca attccgaaaa atcggttaac tgtcaattgc
     6901 aataatgtcg ctaatccaca cataaagata tcaattgaaa tcaaataagt catctgttct
     6961 tgattaaagc ctagtcctgt accgatcaaa agaggcaccg ctactgctcc tgcatacatc
     7021 gctagcagat gctgcagccc taaaaccgca gcttttccat tttgtgtttc tttttccacg
     7081 attatgcgtc ctcctcaaga aactcaactt gtccattcgt caaggaagca atacgcgcaa
     7141 gagaaacgac tctcaatccc atttcttcaa gtaactgacg tccatcttgg aaagactttt
     7201 cgatgacgat cccgattcct tctacttgcg ctccagcttg ctggcataat tcgatcaatc
     7261 cttttgccgc ttgtccgttt gccaagaaat cgtcgataat caatactttg tcttctgaag
     7321 ataaaaattt acgagaaata gaaatcgtac ttgtcacttg tttcgtaaaa gaataaacag
     7381 aagctgtcaa taattcttca tccatcgtca aactttttgc ttttctagcg aagatcattg
     7441 gaacatctaa ttctcttgca gcaaataaag ctggtgcgat cccagaagct tcaatggtca
     7501 caactttcgt gattccttgt tctttaaata cttctgcaaa ccgttttccc atttgttcca
     7561 tcaatacagg gtctacttgg tgagtgacga agctgtcgac ttttaatacg ccctcgccta
     7621 atatacgtcc gtctttttga atacgttcaa ctaattcttt cactttattt gctcctttat
     7681 catttattaa taccgaaata accgcttttg tgcaaaaacc tactatgttt ctgttaaaga
     7741 aaaacaccct ccactttatc ataagaaaag aagggtgaaa tagccaatct tttacttata
     7801 gtccagcatt tacggtactg gctagaaact gtcatccata ttatgacga
//
LOCUS       DANKKG010000119         7700 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000119 DANKKG010000000
VERSION     DANKKG010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.119"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            62..2635
                     /locus_tag="PW004_002266"
     CDS             62..2635
                     /locus_tag="PW004_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727219.1"
                     /translation="MNQYVKKLIASILFVFPLLMVTSVFADVEKTSESTQSENQSFDK
                     TTFSEESITSETTESQSEAPTSNTRQEENKTSETSEVAEQTSDSSEEVQVRVVSADGY
                     SEAATTEELTQLLADESVAKIRLTQPLTLDRELEIKRDIVIDFGGLAHNFGTHHIYIN
                     ETPQSIEFQNFKGTAAHPGGLIPNIDGNAIIIAYMSDFWGNRYHFTGEIKFTGTLDLY
                     DGSKLGLIYAPRATVTLDGVSGVLDVQPVKEGMNAAPGKAYFCRSYQLNVINGSQLYG
                     PYLGKFYGFLDSGDETGSGKTAPGLHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVT
                     FKVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSRVAVSNDGKITINTATTGGFRLQG
                     DSSQIHVSSGGQISVKMAGDGDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSA
                     VRFENGTQKLTVTNGGSLKVRNAGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFT
                     VTGEKSNIDIRADSGATVDTTGDIDLNFMAGEKTYLVMVGKTGEDKTGIISGDVLNVD
                     LESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKAGVTFDNFEKDP
                     DYFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRV
                     PTNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSPVEITALTQGQTDE
                     NEGTGYNPWGEGEQGGLFEVQVPNEERLTTGDQVQIVAAKKVRGGQEVTELDSAKTTV
                     DVIPPEPAKLSTTLINAASRSITGTGEAGALVSLKKNQAVLSETTIDDTGKFVLSIPE
                     K"
     gene            complement(2708..4255)
                     /locus_tag="PW004_002267"
     CDS             complement(2708..4255)
                     /locus_tag="PW004_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HDL2727220.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(4357..4710)
                     /gene="tnpB"
                     /locus_tag="PW004_002268"
     CDS             complement(4357..4710)
                     /gene="tnpB"
                     /locus_tag="PW004_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HDL2727221.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(4700..4894)
                     /locus_tag="PW004_002269"
     CDS             complement(4700..4894)
                     /locus_tag="PW004_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727222.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
     gene            4977..5585
                     /locus_tag="PW004_002270"
                     /pseudo
     CDS             4977..5585
                     /locus_tag="PW004_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5605..6633
                     /locus_tag="PW004_002271"
     CDS             5605..6633
                     /locus_tag="PW004_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HDL2727223.1"
                     /translation="MNRIWNKSHRNNCSLLLILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILIRNSEKESSPNLKLKKTYADQSDGRNVIKANLQNPNAS
                     YMFGLTTSVEIYKKETKEPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKG
                     AWKEKRWNLHTDFEITDDAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFI
                     LKERRKKK"
     gene            6630..7388
                     /locus_tag="PW004_002272"
     CDS             6630..7388
                     /locus_tag="PW004_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HDL2727224.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPNEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QNGTDSTGTATQVQNFVQVTDKSGIYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGTPSTISGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEGKA"
BASE COUNT         2488 a         1455 c         1605 g         2152 t
ORIGIN      
        1 attacaaata aattaaaacg ttaaaataaa attgtcaaaa cgtcagagag gatggtggag
       61 aatgaatcaa tatgtaaaaa agctgattgc gtcgattttg tttgtcttcc ctctcttgat
      121 ggtgacatcc gtttttgccg atgtagagaa aacaagcgaa tcaacgcaat cagagaatca
      181 aagttttgac aagactactt tttcagaaga atcaattact tctgaaacta cagaatccca
      241 aagtgaggcg cctacttcta atacaaggca agaagaaaat aagacaagtg agacctcaga
      301 agtagctgaa caaacgtctg atagcagtga agaggtacag gttagagttg tttcagcgga
      361 tgggtacagc gaagctgcca caacagaaga actgacgcag cttctcgcag acgaatcagt
      421 agctaagatt cgtttaactc agcctttgac tttagacagg gaattggaga tcaagcgaga
      481 tatcgttatc gattttggcg gacttgctca taatttcggc acacatcaca tctatatcaa
      541 tgagacacct cagtcgatcg agtttcagaa ttttaaagga acggcagctc atccaggtgg
      601 gttgatacca aatatcgatg gaaatgccat tatcattgct tatatgtccg atttttgggg
      661 aaacagatat cattttacgg gagaaataaa atttaccggg acacttgact tatatgatgg
      721 ttctaaatta ggcctgattt acgcccctcg tgcaactgtc acattagatg gtgtttccgg
      781 tgtattggat gtccaaccag tgaaagaagg gatgaatgcc gctccgggta aagcttattt
      841 ttgtagaagc tatcagctaa atgtaatcaa tggtagtcag ctttatggac catatctagg
      901 aaaattctat ggttttctag attcaggtga tgagacgggc agtggaaaaa cagcaccagg
      961 actccatatc ttgagtggtt caaaggtctc tcttgactat gatcgggcag atggcgtcag
     1021 cgatgaagga gaggctgtcg atacgctacc cgggaatgtc acatttaaag tcagtggctc
     1081 aggaagtgaa ttttcggtaa atacaaagat caatattacc aacgacaaca atcgcggaat
     1141 tatccaaatg agaggctcag gatcaagagt cgctgtgtcg aatgatggga aaatcacaat
     1201 caatacagcg actactggcg gatttcgcct gcaaggagat tctagtcaaa tccatgtatc
     1261 aagtggtgga caaatctcgg tcaaaatggc tggagatggc gaccaacctg gaaacaatgg
     1321 gatgcgtttt gtagggaaag gactttcttt gatcgtagaa ggagccggat cagtcatcga
     1381 tattgataag caaagtggtc gttctagcgc agttcgtttt gagaacggta cgcaaaaact
     1441 aacagtaact aatggaggct cattgaaggt aagaaatgca ggggatggca aatcttatgt
     1501 gaacagactc aatcaaggaa accaagcgat ccaattttat gacgcttctg gttttctaga
     1561 aagtccaggg tcagctgatt ttacggttac tggagaaaaa tcaaatatcg atatacgcgc
     1621 agattccgga gctaccgtag atacaactgg agatattgat ttgaacttta tggctggcga
     1681 aaaaacttat ttagtcatgg tcgggaaaac tggggaagat aagactggca tcatcagtgg
     1741 ggatgtgttg aatgtagact tagaatctcc tacctatttc gatttccaaa acaaacggat
     1801 cggaagcgag actaatggca taggtggctg ggtatttcaa ggaaatggcg attcttcttt
     1861 gaagatcgct cattcagaaa ttgctttatg gcgaaaggca ggagtaacgt tcgataactt
     1921 tgaaaaagat ccggattatt tttctgatat gacagactta tcgatcaatg gaaccgactt
     1981 aggtaatttt gtatcaagcc ctagtgcagg tttaacaaat gaatttgctc agacaggtgc
     2041 cggaatgaaa aattataatc gaatcagtgc caataaccag cttccgatga ttgaatcctt
     2101 aagagtacca acgaatgcag ataaaaaaat ctatgggcat gtcgttgttc ccgaaggtgt
     2161 cgaggcaaag ccaagagatg cttggacagg cgaagtagaa gtgactttac gtattactta
     2221 tgcagatacg acgaaatcac ctgtagaaat tactgctctc acacaaggac aaacagatga
     2281 aaatgaaggg acaggatata atccgtgggg agaaggcgaa caaggcggtt tgtttgaggt
     2341 tcaagtaccc aacgaagaaa gactaacaac gggggatcaa gtgcaaatag ttgctgctaa
     2401 aaaagtaaga ggcggccaag aagtgacaga actggactct gccaaaacga cagtcgatgt
     2461 gattcctcct gagcctgcta aattaagtac cacattgatc aatgcagctt ctcgctcaat
     2521 cacaggtaca ggcgaagcag gagctttggt ttcattgaaa aaaaatcaag ctgttttgtc
     2581 cgaaacaact attgatgata caggtaaatt tgttctttct atcccagaaa agtaagcgcc
     2641 ccatagaaca gtacctgtac gtcaaataac ccgacctata gaatagaatc gggaatgttt
     2701 ggcgttttta gttacgagaa aaatgttgtt gtacatcctt gttccaaggc aacagttgat
     2761 ctaaagtttc tgctgaaagt acgggaagat ttggtatctt atcaaataga aactctagat
     2821 actttacagg attcaatccg ttaagttctg ctgttttata tacactcaga atgattcctg
     2881 aagttcgtgc tccttcaaga cttgtggaat gcaaccaatt tttccgtcct atcaccagct
     2941 ctttgatatt tcgctccgca cgattatttg tgagttctaa acgaccatct tcaaaaatag
     3001 gttcaaaacc acttcgtagt ttacacgcat attgaacggc tgcatccaac ttggattttg
     3061 caacaggatc tatcgttgcc atccactcat aaaaagcaat aaataatgga cgcagttctt
     3121 gttgccgaca gcttaatcgc ttctcaggag aaaagtcttt ccatttcttt tctaggacaa
     3181 accatttatc caattgtttg actgcttgag ctgcgggaat atccgtgttt ttctttcctt
     3241 ttggaattgc ttcgaaaaat ttccgccgaa catgcgcgaa acaatagaca agtcgtacac
     3301 tttccacggc attatacccg ctatagccgt cacagtgtaa ataccctgta aactccttga
     3361 gaaaagtttt cggaacatct ccagcacgac tttcgtcgtg gtgataataa acgatgggat
     3421 gctgactgtc ttttcctgta caaaactgcc agtaataggt atctgttttg gcactttcaa
     3481 tcacacgata acttgtttca tccccgtgta gaatctcttc ttgattaaga tagactctta
     3541 atcgttctcc taaagggtct aacgcgtttt gcactgcttt aatatgccaa ttggtgatat
     3601 tatctctcga aatatccaac ccatgtgttt gtttccaata gttctcttgc cgataggcag
     3661 gaactttctg accaaatttc atccggatcg tttccgccac taaactattg gaacctaagc
     3721 tattagaaat caaaggttta ggaacgatcg cttttttaaa agaagaaagt ccttcttttt
     3781 cgcagctttt acaacaatag gtatgttgat aatgggtgag acaatccaac atagcctgat
     3841 ggaaacgaat ttcttctcgt acttttgtcc gtccgatatc tttcatctgt gaaccgcaat
     3901 gttcacaaaa ttgttcttcc aaaggaagga gatgttcttt ctctgttata ggtaagtgtt
     3961 tgattttgtc tgctttcgtt cctacaactc gttgatgtcc ttttactgga ataatggtgg
     4021 cagctgcttc tggaacactt acagaaattt cttcatcact aaaaagattc aattgcccat
     4081 tcgtttcctc aaagagagtc tctttagagc gtccaaataa tttgttggtt aaataattga
     4141 ccttttcatt caatagttgg atttcagttg taagattctg aatggtttgt tgttgtgctt
     4201 gattggtttt atgggcttct tgaagcaact gaagcaacgt atctgtagtt tccaaaattt
     4261 ttcaccacct tgaatcgaac tgattttgtt acagaatacc acaagaaacg cacaagaaat
     4321 aggggttcaa cacatttttt gtgttaaccc cccagcttaa aaaactcctt tttttcctgg
     4381 caaaatagct cgaggttgat cgatacttaa tccttctaac aaccatcgaa cctgttggtt
     4441 cgttaaggtt cgaacttcgt ctttcgtccg aggccatttt aacttgccgt tttctatccg
     4501 cttataacag agaagaaaac catcaccgtc ccaatagaga agtttgaaac ggtcttgacg
     4561 gttcccgcaa aataaaaaaa cggcatcttc gtaaagctcg agttgatact cttgttgaat
     4621 aagactggca agaccatcaa ttccttttcg caaatctgtt tttccgcaca caatataaat
     4681 atgtttggtt tgaagtaaat cataaagcat tggacaacag ttctttcaaa atagcttgaa
     4741 gaatataatt gttaataccg ttatagatca aaacttcaga accattctct cttttgatgc
     4801 gcatcgctaa cttggcagcc gcgggtttac ctttttctgg tgcactcttt tcgtttccta
     4861 attggacagg aacaatctca tttaatttat acatagaaaa cctccagact tcttgatagc
     4921 tcaagtatat cttgaggtgc atttgttagt aaggtactgt tctatggggc gcttacccag
     4981 aaaatcaact ttttgcagga gatgtattag aaattgtatt aaacgaccaa gcaggagaag
     5041 ctgctgctaa gggtgtgcag aacaagccaa gtaccaacga tgaaattggg aatcacaatc
     5101 caagtgatac gccagttgtt tatcatgatg ccccgccttt tgcacctgct acgaagattg
     5161 tagtagaagg tggtctctca tttcttgccc cttccaaact cgattgtggt cagatcaaag
     5221 caactgggtt gacgcaagaa gcatttggcc aaatggatat acaggaaaaa ttatggattt
     5281 atgatcagcg agaaacaaaa agtccatgga tcttaacgat aaaattatct cagcctttca
     5341 caaccgatgc aggattttct atgcaggatg tcctatattt caagaaagct gaggagtatc
     5401 aattagtgaa tgaagaagaa ctggaaattg tcagaggaca aaattcaacg aatgtgcaaa
     5461 cagattattc agaatattta aataatggtc gggcgtttaa acttgtctta accaaagaaa
     5521 atcagatagc tggtaattac tcagcagaga tgacatggac attagcagat gcgccgacag
     5581 aataaagatc aaggagaatg attgatgaat cgaatatgga ataaaagtca tcgaaacaac
     5641 tgctctttac ttctgatcct tctccttgga ttcatccttt ggcagacacc tgtccatgcc
     5701 tccgaattca attttgcggt agattttgaa ttaccggcta atcagattga taagaacaaa
     5761 acatatataa atgtaaggat ggagcctggg aaaacacagg aaatccccta cacggtcttt
     5821 aaccgaacag ataaatcagt aacagttgag acattgatcc actccgctac gacgaattca
     5881 aacggtgtga tcgaatatgg agaatcaaaa agtaaatttg atgaaacatt aagttatgat
     5941 atgaaaaact tagcaaaaac agtgggagaa ataactgtcc ctcctcaaag tgcggtcact
     6001 gagacacttt cactgaaagc tccagaaaaa gagctggctg gtgtggtcgc aggaggaatc
     6061 acgtttcaag aaaaaaaaga gaacgtttct aacaaaagta cagttggtgt cgtcaatcag
     6121 ttttctcaag tgaaagcaat cttgattcga aatagcgaaa aagagtcttc cccaaatttg
     6181 aaattaaaaa aaacttatgc ggatcaaagc gatggaagaa atgtgattaa ggcgaatctt
     6241 caaaatccaa atgcttctta tatgttcggc ttgactacat cggttgaaat ctacaaaaaa
     6301 gaaacaaaag agccttatct ttcaataaaa aaagaaaaaa tagatgtagc acccaataca
     6361 aattttgatt tacttgtccc attagaagga aaaaaattag ctccgggaca ttatcaagcg
     6421 gttgtgaagg gtgcttggaa agaaaaacgc tggaatctcc ataccgattt tgaaataact
     6481 gatgatgcag cggaccaatt gaatgaaaag gatattgatt taaaaaatca gccagaaaca
     6541 tttgactggc gatggctgat acttgttgga gtcattctta ttggcttatt tgctggatta
     6601 tttattttaa aagaaagaag gaaaaagaaa tgaaaaaaac aattgcatta agtaccgttc
     6661 tagctggaag tctattatta tgtacacaaa gtgtttttgc tgagaataag agctcagcca
     6721 ctacagaagg aacgatttct gtaacaaaac cagatgccgc aaatcctgaa gacgttgttc
     6781 cagttcctcc tgttgatcca aatgatcccg atacgccgtt tacacctaac gaaccaaatc
     6841 agggaacatc cggattactg acgattgacc aagctccaga tcttgatttt ggaacaatca
     6901 agttaggtac agcaaaaaca gtatatgctg cattacaaaa cggaacggac agtacaggga
     6961 cagcaacaca agtacagaat ttcgtacaag taacggataa aagcggaatt tatgccggtt
     7021 ggaaactatc agtcaaacga acagaattta ttggtacaac atctgatacg aataaactaa
     7081 ctggttcaaa ggtgaaattc aaaaacgccg tgattcgaaa tagcgcaact aatagcagtg
     7141 gaacgccatc tactatttct ggtactggcg ctgaaggaat tgagatccca gcagatcagg
     7201 cagtagatct agtaactgca ggagcaaatg aagggattgg cacatggatt tattcattag
     7261 gagagaatgt ggaacaaggg agacaatcag tggaattatt tgttcctgta tccaattatg
     7321 ccgtagacaa ctacgtttca acattcacat ggtcgttaac agatgctcct acagaaggga
     7381 aagcgtaaaa tcaaatcaat gacttcaggg agtggagccg attcaaggcc aactccctga
     7441 aatctgctat gcttacgtct cagctcttat tcatcaactc gaagcagttt tttggaagtc
     7501 atggaaacgt tcctttgtct tttcaaagat ctttcgacca acgagtaagg agtcaccatt
     7561 gaaaaattct aacacataat gagcagaatc aattttctgc aattgcgata agttgataca
     7621 gtaagatttt aatatcttaa ttgtcaaatt aagttagaca gatcatcaat acttacgtaa
     7681 ctcaaaaata cttccaaagg
//
LOCUS       DANKKG010000120         7618 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000120 DANKKG010000000
VERSION     DANKKG010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7618
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.120"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(<1..683)
                     /locus_tag="PW004_002273"
     CDS             complement(<1..683)
                     /locus_tag="PW004_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727225.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVIINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFILLF
                     VERFNQAFHTDYATNIFEDFENSYSYSVISSTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLL"
     gene            809..1474
                     /locus_tag="PW004_002274"
     CDS             809..1474
                     /locus_tag="PW004_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2727226.1"
                     /translation="MNIGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHQTLGLPIDFPSIESATESIEPTLPDEPLVIKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETVDRFNPFLKPQIGYTTLPVGYVENSIEHWVCNDIFVHGTW
                     LSFHAGEVEKGKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILIQDYDDALKRSPQEP
                     DRH"
     gene            complement(1871..3904)
                     /gene="brxL"
                     /locus_tag="PW004_002275"
     CDS             complement(1871..3904)
                     /gene="brxL"
                     /locus_tag="PW004_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HDL2727227.1"
                     /translation="MEQQEILKLSEFFPGKIVRKDLTKKIKEGANVPVYVLEYLLGMY
                     ASSLDETLVEKGVENVKNILARNYVRPDEAEKVKAKIRELGGYTVIDKVTVHLNEKKD
                     CYEAEFSNLGLKNVQISEEYPKKFDRLLGGGIWCIVSMEYWYDENDRWNSPFIAKKVD
                     PIQMPGLDIEAYKMARKEFTTNEWIDLLLRSIGMEPTQFNKRVKWLHLARLIPLIENN
                     FNLCELGPRGTGKSHVYKEISPNSILVSGGQTTVANLFYNMASRTMGLVGMWDCVAFD
                     EVAGIKFKDNDGVQIMKDYMASGSFARGKEEKNASASMAFVGNINQSVDVILKTSNLF
                     EPFPEVMGTDSAFLDRMHCYLPGWEIPKYRPDFFTNDYGFITDYFSEVMRELRKVSFG
                     DSFDKYFKLGSQLNQRDTIAVRRIVSGMMKLLYPDGRFEKSDVEEILRFALEMRRRVK
                     EQLKKIGGMEFYDVNFSYIDLEEEREEYVSVPEQGGGTLIPEGLGKPGHLYTVSYGKT
                     GQLGTYKLETQMTSGNGKFERTGINSDAGAREAMDSAYRYLRANYGSVSQSISLTTKD
                     FVVHVQDMNGIGMTKTLSLSTIIALCSVSLNKPVLPSLVILGDITIGGSMIKVDNLAN
                     CLQVAKDAGAKKVLVPMISAADIGTVPPELMGTFNLIFYQTPEDAVFKALGVE"
     gene            complement(4022..6544)
                     /gene="pglZ"
                     /locus_tag="PW004_002276"
     CDS             complement(4022..6544)
                     /gene="pglZ"
                     /locus_tag="PW004_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type A"
                     /protein_id="HDL2727228.1"
                     /translation="MTDINTQQIEEKLNQRFPNYGCRKIIFWFDSNQDFLEEIDSLVI
                     QNAKIYRLEKDAQFKTKRLLEYEDPETNYLIYAPFPRWDDKDEDNHLLSVLNYSEEFS
                     ADRLAIIMNELAIPLRFHQTVKQYRNFFNAKDRMRSFGEFSTHMTINKSETIELMIMA
                     VLLKSKSIRLNDLLRVVLKSFTESRENPLRELDKYQLTAKFWEFIQMKFGFTDSQPTL
                     EKLTMCLYLNYFYYQIDQEMPKKFKGFEILHLKANIITFMDQFMNDTRYMESFEQLSK
                     KVYQAIDGDGLLDSIEIDDLIEADVFSEIHAKILMFYAERLKASDTTTKIAGQTIFDS
                     LKQREKMHFASDYIYEYQLLKHAYYLVDKNRLCLPMNMDNLLTQYEKEMYWVDTHYRK
                     FIWNSDHIEEKQKYGQLYRLVEANYSKFLDQSGQIWNQTFEFKNRPSIRKFYNQAVMK
                     RSVKTVVIISDAFRYELGMNLERKLVREKKYSTNMASFLSVLPSVTEFGKAALLPNNQ
                     LTYNEGTEVLIDGKKTNGLSNRNAILQQRDANAVALNYDDVVKMNKAEVREVFNGKSV
                     IYIYHDQVDRTGDHSNENRVFEACDLAISEISRLINRLHNDGSIYRFIVTADHGFIYK
                     RSHVEEVDKIENPSRNEKDRVERRFILSEEEYEIIGTKNYSLGDSLGNDDTRNICVPI
                     TSSIFKKAGGGQSYVHGGSSIQEMLVPVLEITVAKGSNAKEPVAIELMTSNRRITGLS
                     TTLEFYQKNAISDVYDPASFWLYFEDEYGERISNEEIYVADSKTEDANQRFTKFVFEF
                     VNRRYTVEDTYYLVIKNQKNDLTERIKFIIDNPFAESFDFDI"
     gene            complement(6660..7604)
                     /locus_tag="PW004_002277"
     CDS             complement(6660..7604)
                     /locus_tag="PW004_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002397590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727229.1"
                     /translation="MFKKDLSEAFNCLSRQLVNQFNTLKSNEEELNDIFINHYSLEKE
                     LTKEVQDEYISARLPDKAREIKDFISYSIGCIFGRYSLDEDGLIFAGGEWDPSRYQKF
                     KPDTDNVLLITDDEYFKDSQIDIVNRFIDFVEITFGKETLEENLQFIADTLGGKGTSR
                     EIIRRYFVQDFYKDHLKTYSNRPIYWQLDSGKQNGFKALMYLHRYTPDQLGKVRTEYL
                     HELQKAYDNRIELRQQQISTSDNPKERNAMTKEIVKIQKQLKEVREYDEKLGHLALKR
                     IELDLDDGVIVNYDKLQRDPKTGEKFQIFAKGVKEPKK"
BASE COUNT         2461 a         1491 c         1089 g         2577 t
ORIGIN      
        1 ttcaacaata aactgatttc tttattcatc aataagaaaa aggatgataa caaaaggaat
       61 aaaagaatca atccaatagt taaatacact cgataattat atacgtctgt acttgaaata
      121 accgaatacg aatagctatt ttcaaagtct tcaaaaatat tagtggcata atcagtgtga
      181 aatgcttgat tgaatcgttc cacaaatagt aaaatgaacc gttcataggc ttctttttct
      241 ctcgtcatca agaaaaattc accttcaaaa tcttttattt tgatcgattc ttcaatggac
      301 gttacgcgaa aggaggtatt agaatagggt accttctcgt gagaagattt gacatcccct
      361 tgataataca atgtcatttc aagctttggc tgtatcttga agttccaaga tgtatctact
      421 tcattggatt catttactgt ttttttcata taagaaagct cagtttctga tgagacattt
      481 tcaaatacca tgattagctg ctcatattct tcttgcccaa taaaatgttg tggcaatcga
      541 atcggagtat aagtatctgt ttgatggaaa aattcatttg tccgatagag tacctgttta
      601 tttataataa ccaaggcaaa accactcatg ataccggtca ctatgattgt gagtaaaact
      661 gtgaactttt ttttgattat caccactctc ccttacttaa atgattcttt gataaaatca
      721 taccgttttt catcaaaaaa acgtatctat caaatgtata ggtcatgtag aataaatcta
      781 tatactgtag aatcgaaagg agaagaagat gaacatcgga gaaaaaatac gaacttaccg
      841 cagaaattgt aagcttacgc aaaaagaatt aggcgaaaaa atcggtgttt ccgataaaac
      901 aatctctagt tgggaaaatt ctcgtacaat gccagacctt gaaatgctgt cacttttaca
      961 tcagacttta ggtctgccga ttgattttcc ttctattgaa tcagctaccg aatcaataga
     1021 accaacattg ccggatgaac ctcttgttat caagaaatct tattcatcaa aacaaccctt
     1081 ctcactcaaa aagacactat ctttctttct gctcgtcttt tgtttggtct atctttttct
     1141 tatcaattat caatataaaa atgaaacggt tgatcgattc aatccttttt tgaaaccaca
     1201 aatcggctat accacactcc ctgtaggata cgtggaaaat tccatcgaac attgggtatg
     1261 taatgatatc tttgttcatg gtacatggct gtcttttcac gctggagagg tagaaaaagg
     1321 taagaatcat gctgtaatca aacacaaagg aaaacacgtc atgaacgttc gcctgctcac
     1381 ttgggaagcc attccctcag attaccaaga aatcctcatc caagactatg acgatgcctt
     1441 aaaaagaagc cctcaggagc cagataggca ttaagtagga gatgaacgta gagatagaaa
     1501 aaatcaatgc acatgatctt atcaaaaaaa aacaggggta caaattaatt gaaaaagctt
     1561 tgagatgaaa gttcctaaat aaaaaagttc agaatgatag gtaaaaaaat gactaactat
     1621 tctagtatta ggctatttgt attattcttc tgttattttt atatcaaaaa atccagaaat
     1681 aactaaatat aggtatttct ggcttatagt ataatattta cgccacagcc cccaatgatt
     1741 ctttttggaa tcatgatcaa ggacggacat acacaaaaat attttgccca ttcgatccga
     1801 atagattaac gactaatcta gtaagtaata tagattggta cgtagtcaaa ccagcaaatg
     1861 gaaaagttgt ctattccacc ccaagggctt taaagacagc atcttccggc gtttgataaa
     1921 aaatcaaatt aaaggtaccc attaattctg ggggtacagt tccaatatca gcagcgctta
     1981 tcataggtac aagtactttt ttcgctccgg catcttttgc tacttgtaaa cagttagcta
     2041 aattatctac cttaatcata gaaccaccaa ttgtaatatc ccctaaaata actaaacttg
     2101 gtaatacagg tttatttaag gaaactgagc ataaagctat tatagttgat aacgataatg
     2161 tttttgtcat accaatgcca ttcatatctt gaacatgaac gacaaaatct tttgtcgtca
     2221 aactaataga ctgactaaca cttccataat tagctcttaa atatcggtat gccgaatcca
     2281 tcgcttctct tgctccagca tctgaattga tcccagtacg ttcaaacttt ccatttcccg
     2341 aagtcatttg cgtttccaat ttataggtac ctaattgccc agttttgcca taagataccg
     2401 tgtacaaatg ccctggcttc cctaaccctt ctggaatcaa agttcctcca ccttgttcag
     2461 gaacagaaac atactcttct cgctcttctt ctaaatcaat gtatgaaaaa ttgacatcat
     2521 aaaattccat tccaccaatt ttctttaatt gttcttttac acgacgacgc atttcaagag
     2581 caaatcttag tatttcttcg acatcacttt tttcaaatct accatcagga tacaacagtt
     2641 tcatcatccc agatacaatc cgacgaactg caatcgtatc ccgctgattt aattgagaac
     2701 caagtttgaa atatttatca aaactatcgc caaaactaac ttttcgaagc tcacgcatga
     2761 cttcagaaaa ataatctgta atgaagccat agtcatttgt aaaaaaatct ggtcgatatt
     2821 taggaatttc ccaacctggt aaataacaat gcatccgatc caaaaaggct gaatccgtcc
     2881 ccataacctc cggaaatggc tcaaataaat tcgaagtttt caatattaca tcaacgcttt
     2941 gattaatatt tcctacaaaa gccattgatg ctgaagcatt cttttcttct ttgcctctag
     3001 caaaagatcc tgaagccatg taatctttca tgatttgaac gccatcatta tccttaaatt
     3061 taatcccagc aacttcgtca aaagcaacac agtcccacat acctacaagt cccattgtac
     3121 gactagccat attatagaaa aggtttgcta ctgttgtttg tccacctgaa acaaggattg
     3181 agtttggtga aatttcttta tatacatgcg atttacctgt accacgtggt cctagttcac
     3241 agagattaaa attattttca attaatggaa taagtcttgc taaatgaagc cattttaccc
     3301 ttttattaaa ttgtgtaggt tccataccaa tggaccgtag aagtaagtca atccattcat
     3361 ttgtagtaaa ctccttgcga gccatcttgt aagcctcaat gtctaaacca ggcatttgga
     3421 ttggatcaac cttttttgca ataaaagggc tattccaacg atcattctca tcataccaat
     3481 attccatcga aacaatacac cagatccccc cacctaataa tcgatcaaat tttttcggat
     3541 actcttcact aatttgaaca ttttttaatc ccaggttaga gaattctgct tcataacaat
     3601 ctttcttttc atttaaatgt acagttactt tatcaataac tgtataacca cccaattcac
     3661 gaatttttgc ttttactttt tctgcttcat caggacgcac atagtttcga gcaagaatat
     3721 ttttaacatt ttccacacct ttttcaacca atgtttcatc caatgacgaa gcatacatac
     3781 caagtaaata ttccaataca taaacaggta cattcgcacc ttctttaatt ttctttgtta
     3841 aatcttttct gacaatcttt ccgggaaaga attcagataa ctttaaaatt tcctgttgtt
     3901 ccactagaat tcctccatgt gttcagtcat tataatagca attaaagtat atcatactta
     3961 accagtaggc ttgataatac aaaagaaaaa gtatccacaa aaaattgtgg atacctcgtt
     4021 tttatatatc aaaatcaaat gattctgcaa aaggattatc tataatgaat tttattcgct
     4081 cggtcaaatc atttttttga ttttttatca ctaaataata agtatcttca actgtatatc
     4141 gacgatttac aaattcaaaa acaaatttag taaaccgctg atttgcatct tctgtttttg
     4201 aatccgcaac atagatttct tcatttgaga tacgttcacc atattcatct tcaaaataaa
     4261 gccagaaaga tgcggggtca taaacatcac taattgcatt tttttgatag aattctaacg
     4321 tggttgataa tcctgtaatt ctgcggttgc tagtcattaa ttcaattgct actggttcct
     4381 ttgcattgct acctttggct accgtaattt ctagaacagg gactagcatt tcttgaatag
     4441 atgaccctcc atgaacgtag ctttgtcccc cgccagcttt cttaaaaata cttgaagtaa
     4501 tcgggacaca aatgtttcta gtgtcatcat ttcctaaaga atctcccaaa gagtaatttt
     4561 tcgtaccaat tatttcgtat tcttcttcac taagaataaa acgacgttct acacgatctt
     4621 tttcatttct agatggattt tcaatcttat ctacttcttc aacgtggctt cttttataaa
     4681 taaatccatg atcagccgta actataaaac gatagatact gccatcatta tgcaagcgat
     4741 taataagtcg ggaaatttcg cttatcgcta aatcacaggc ctcaaaaact cgattttcat
     4801 tcgagtgatc acctgttcta tccacttgat cgtgataaat ataaatgact gatttgccat
     4861 taaatacttc tcgtacctca gccttattca tcttaaccac atcatcataa tttaaagcta
     4921 cagcatttgc atctcgttgc tggagaatcg catttcgatt ggataaacca ttcgtttttt
     4981 tcccatcaat cagtacttct gttccttcat tgtaagttag ttgattgttt ggtaataagg
     5041 cggctttacc gaattcagta actgaaggta atacagaaag aaagctagcc atgtttgttg
     5101 aatacttctt ttctctaact aacttacgtt ctaaattcat ccctaactca tatcgaaaag
     5161 catctgaaat aataactaca gttttaacag agcgtttcat tacagcttga ttataaaatt
     5221 ttcgaatgga tggacgattc ttaaattcaa aagtttgatt ccaaatttgt cctgattgat
     5281 caagaaactt cgaataattt gcttcaacta atcgatacaa ttggccatat ttttgctttt
     5341 cttctatatg atcactattc caaataaatt tccgataatg cgtatcaacc caatacattt
     5401 ccttctcata ttgagttagc aaattatcca tgttcatcgg caagcacaat ctgtttttat
     5461 ccacaagata ataagcatgt ttcaataatt gatactcata aatataatcc gatgcgaaat
     5521 gcattttttc gcgctgcttt aaactatcaa aaattgtttg tcctgcaatt ttcgttgtag
     5581 tgtcagaagc ctttaatcgt tctgcataaa acattaatat ttttgcatga atctcagaaa
     5641 atacgtctgc ttcaattaga tcatcaattt ctatactgtc taataatcca tcaccatcaa
     5701 tagcctgata tactttttta gatagctgtt caaagctttc catgtaacgt gtatcattca
     5761 taaactgatc catgaaagta ataatgtttg ctttcaaatg tagtatttca aatcctttga
     5821 acttcttcgg catctcttga tcaatttgat aataaaaata gttcaaataa agacacatcg
     5881 tcagcttttc caatgttggc tggctatccg taaagccaaa tttcatttga ataaattccc
     5941 aaaatttagc tgttaactga tacttatcaa gttcccttaa aggattctcc ctactttctg
     6001 taaagctttt tagaactaca cgcagtaaat catttaatcg aatacttttc gatttcaaca
     6061 aaacagccat tatcattaat tcaatcgttt ctgatttatt aattgtcata tgcgtactaa
     6121 attcaccaaa acttctcatg cgatcctttg cattaaagaa attacgatac tgtttaactg
     6181 tctgatgaaa tctaagtgga atagccaatt cattcatgat aatcgctaag cggtctgcac
     6241 taaattcctc tgaataattc aatacactca acaaatgatt atcttcatct ttatcatccc
     6301 aacgaggaaa tggcgcatag attaaatagt tagtttcggg atcttcatat tccaataaac
     6361 gcttagtttt aaattgtgcg tccttttcta atcgatagat ttttgcattt tgaatgacca
     6421 atgagtcaat ttcttcaaga aaatcctgat ttgaatcaaa ccaaaaaatg attttcctac
     6481 atccataatt aggaaaacgt tgatttaatt tttcttcaat ttgttgcgta tttatatctg
     6541 tcatcaatct cacctaatcg ttctctttct atacccctta tattgacata atttggataa
     6601 tttcgcacgc aatttaagaa taaaaaagaa agttgttaga aaacaacttt cttagataac
     6661 tatttttttg gttctttgac cccttttgca aaaatttgga atttctcgcc ggttttagga
     6721 tcacgctgca acttatcata attgacaatt acaccatcat ctaagtctaa ttcaattcgt
     6781 tttaatgcta agtgacccaa cttctcgtca tactcacgaa cttcttttaa ttgcttctga
     6841 atctttacaa tttcttttgt cattgcattt ctttcttttg ggttatcact tgttgaaatt
     6901 tgttgctgac gcaattcgat tcgattgtca tacgcttttt gtagttcgtg taggtattca
     6961 gtacgtactt ttcctaattg atctggtgta tagcgatgca agtacattaa cgctttaaag
     7021 ccattttgtt ttcctgagtc caattgccaa taaattggtc gattggagta tgtttttaaa
     7081 tggtctttgt aaaaatcttg tacgaagtaa cgacgaataa tttctcgact tgtccctttg
     7141 ccacctaaag tgtcagcgat aaactggaga ttttcttcaa gggtttcttt accaaaagta
     7201 atctcaacga aatcaatgaa acgattgaca atgtcaattt ggctatcctt gaaatactca
     7261 tcatcggtga tgagtaaaac attatcagta tcaggtttaa atttttgata gcgggaagga
     7321 tcccattcgc cgccagcgaa gatgagacca tcttcgtcta gggagtaacg accgaagata
     7381 caacctattg aataagaaat aaaatcctta atttccctcg ccttatcagg taaccttgca
     7441 gagatatatt catcttgaac ctcttttgtt aattcttttt caagagaata atgattaatg
     7501 aatatgtcgt tcagttcctc ttcattcgat tttagagtat taaattgatt aaccaactgc
     7561 ctacttagac agttaaatgc ctctgagaga tcttttttaa acatataact taacaaaa
//
LOCUS       DANKKG010000121         7598 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000121 DANKKG010000000
VERSION     DANKKG010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.121"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            331..804
                     /locus_tag="PW004_002278"
     CDS             331..804
                     /locus_tag="PW004_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDL2727230.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            801..2933
                     /gene="feoB"
                     /locus_tag="PW004_002279"
     CDS             801..2933
                     /gene="feoB"
                     /locus_tag="PW004_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HDL2727231.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            2935..3069
                     /locus_tag="PW004_002280"
     CDS             2935..3069
                     /locus_tag="PW004_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HDL2727232.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            3163..3807
                     /locus_tag="PW004_002281"
     CDS             3163..3807
                     /locus_tag="PW004_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2727233.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            3994..5187
                     /locus_tag="PW004_002282"
     CDS             3994..5187
                     /locus_tag="PW004_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HDL2727234.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            5180..6907
                     /locus_tag="PW004_002283"
     CDS             5180..6907
                     /locus_tag="PW004_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HDL2727235.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            7301..7498
                     /locus_tag="PW004_002284"
     CDS             7301..7498
                     /locus_tag="PW004_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HDL2727236.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            7500..>7598
                     /locus_tag="PW004_002285"
     CDS             7500..>7598
                     /locus_tag="PW004_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HDL2727237.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQK"
BASE COUNT         2488 a         1290 c         1673 g         2147 t
ORIGIN      
        1 aacaacaaac actacatatt gtgttgtgga aactgtggaa tactaagttt tgtgtttttg
       61 aaaaaagagc tgaaaacaag tatcgtgtgg gaaaatagca aatttaatag agaaaaaatt
      121 gtggaaaact atgaagaaat atttttttct catttttatc tatcaatact aaagattcgc
      181 tcataatatc acaagtataa ttgacattct caattaccga gagtattcta tgtgagaatg
      241 attctcattt atttgcgtaa cacgtaagaa aattagaaca tataatgaat gattttaata
      301 catgatttat ttcacaagag aggaatcaat atgcagacct tagcgcaggc aaagcagaat
      361 ttcgtttatc aagtcgaacg aatcgaaaca gaagaaaaaa caaaacgcca tttgcaaaat
      421 atcgggatcg ttgccggaag taaaatcgtc gtcgtcaatt tatcaggcaa caatgccatt
      481 ttactggtaa aaagtcatcg agtggcagta agtcgggaaa tactaggtca aatctttgtc
      541 aataaaaaga ctgcttcctc tgaaagctgg acttctcttg atcaattaaa agcaggagag
      601 actgggatcg tcgtagcaat ccacggacaa ggcgcagtga agcgacggtt aatggatatg
      661 ggattgacgc gaaatgtaaa aatagttatc cgaaaactag cccctctggg agatccaatc
      721 gaactgacag tgcgagggta tgaactgacg ttacgaaagt cagaggccga actaatcttg
      781 atacaaaggg aggaaaaaga atgaatatcg cgttagtcgg aaatccaaac agcgggaaaa
      841 ctagcttgtt caatctcttg actggttcga atcaatatgt agggaattgg ccgggcgtaa
      901 cagtagaaaa aaaagaagga tcgttgaaaa aaaatccgaa cattcatatt caggacctac
      961 ccggcatcta ttctttgtct ccttatacac cagaagaagt gattgcaaga gattatttgg
     1021 ttcaggaaca gcctgaactg atcatcaaca ttattgatgc taccaattta gaacgaaatc
     1081 tttatttaac tacgcagtta ttggatttag acattcctat gatcattggt ctgaatatga
     1141 tggatgtcat caaaaaagaa ggaaaaaaaa tcaatctaga aaagctttcc tatggtcttg
     1201 gtgtaccagt tttaccaatc agcgtattga aaaagcaagg aatcgaaaaa ctaatcgaaa
     1261 agattcagga gtctgcaaaa aagcggcctt ctattcctgt tccctgtaca tatgatccac
     1321 gtttagaagc agctttacat gaaattgaag atgtactagg agaacaaagc ggcaagaaac
     1381 gctggtatgc aatgaaatat tttgaacgag atcagaaggt aagagaggat tacgagatag
     1441 cagtttctca gcaaaaagag atcgagcaac tgatccagtt aacagagaaa ctattggacg
     1501 acgatagtga gacgattctg gtcaatgaac gttatgaatt tattacccaa ctatgtacac
     1561 tctgtgttgt gtcaaatgat tctttccaat tatcgatgag tgacaaaatc gatcagattg
     1621 caacgaatcg ttggttagcc ttgccgatct tcgcatttgt gatgtggctg atctattatc
     1681 tggctattca aacagtagga acaatgggga cagattggat caatgacaca ttgtttggta
     1741 catggttacc agaacatgtt ggcagattgt tagctgaatg gcaagtagcg ggatggatgc
     1801 aggagctgat cttgaacggc atcatcgcag gcgtgggagc agtgttagga tttctaccgc
     1861 aattgattat attgttttta tgcttgagct ttctggaaga ctgcggatat atggcgagga
     1921 tcgcttttgt catggatcga cttttcagga aattcggtct ttcaggaaaa tccttcattc
     1981 ctatgctgat tgctacagga tgtggagtgc caggcgtcat ggccagtcgc acgattgaaa
     2041 acgagcaaga tcgtcgtttg acgatcatgg tcacaacctt catgccttgt tctgcaaagt
     2101 tgccgattat tgccttagta gcaggggcct tttttcctca tcaatcttgg gtagctcctt
     2161 ctgcctattt tcttggtatg actgcaatta ttttctctgg aatcagcttg aaaaaaacac
     2221 gtttgtttgc tggagataca gctcctttta tcatggaatt gcccgcatat cattttcctc
     2281 aatggtcagc ggtcctaaga caaacctatg accgcagcaa gtcttttgtt aaaaaagcag
     2341 gaacgattat ctttgtttct agtatcgtga tctggttcag ctcttcttat aatttttatg
     2401 ctcaacctgt ttctgaagac caaagtatct tagccttttt cgggcgaatc ctggcagttg
     2461 tttttcagcc gcttggttgg ggaaactggc aaggaacagt cgcagcgatt acaggattgg
     2521 tcgctaaaga aaatattatt ggcacatttg gtattttgtt tggtcatgcg gaagtatccg
     2581 aaaatggcag agaaatctgg caagtgctac aacacacata tactcccgca gcagcttatt
     2641 cattgttagc cttcaattta ctttgtgcgc cttgttttgc ggcaattggg gctatccatc
     2701 gggaaatgaa tgccaagaaa tggacttgga tcgccatagg ctatcaatgc ggtttagctt
     2761 atttagttag ttttgtgatt tatcagttgg gacacttatt ggagggtgga caggtagcgt
     2821 tagggacgta tcttgctatt ttactgatga taggactcgt ttatgttttg ttccgtcagc
     2881 caaaatccaa gaaccagttt tatactatat tgtcattaga aggagaggaa taggatggcg
     2941 acattcttat taagtgtgtt aatctttgga agtgcaggcg caattgtcta tactcgttta
     3001 aaatccggta agaattgtga tgactgtcaa acagcttgcc cagtaaaaaa ggaacagatc
     3061 aagcgatgat cagtcgttgc aagaggctat tctccccttt tttgcttatt ttgctagagc
     3121 atagtaagat gagggaaagt aaaaaaagaa agaggaaaga aaatgtctgg aaagaaaaaa
     3181 atcgtccgcc cgcgctacca gcagattgct gttgatttag ccgaacgtat cgttgaaaac
     3241 cgatataaag tgggtgagaa gatccatgcc cgctcaacac ttgccagtac gtacaatgct
     3301 tctcctgaga ctacccgcaa ggctatcaat gtgctggtag atcttggaat catggaagta
     3361 cggcatggaa gtggggcatt tgttgcttcg aaggaaaaag caaaagattt cctattgcaa
     3421 tatcaagatg tacaatccat ccaagataca aaagcagaaa tcatggcaag tgtggagaag
     3481 caacaagatg agctgaatca tttttctcaa ttattggatc agctggtcaa tcaaacgaag
     3541 cgaatacata agatgaatcc tttacttccg tttgaattat ctttatcaga aaaagcaatg
     3601 catctggacc gttcaattag cgagttgaat atctggcagg cgactggcgc cacagttatc
     3661 gcaatttctc ataatgaaga actgcttgtt tcacctggcc cctatgctaa atttacggca
     3721 ggggacatcg ttcattttat tggaaatgaa tatacactac agcgtatgac aaactttttt
     3781 tacccggagc atcagcttgc agaatgattg tgtgaaatat taaaaaaata ttaacttggc
     3841 taaacctttt atttacaaag atttagccaa gtttttttca gtttggacaa gcaaatttct
     3901 ttcatttttg acaaagtgac tacatgatgt taacatagtg tggtcacttt tttcagaaag
     3961 aaaaatgaat ttacataaaa aaggagaatt tgtttggtaa aagtagaagt aaaacattta
     4021 acaaagatat ttggtaaaaa gacacaagcc gctttagata tgatgaatga tcaccagccg
     4081 aaaacggaga ttttaaagaa aaccggtgca acggttggtg tgtatgacgt gaattttgac
     4141 gtaaaagaag gagaaatctt cgtgatcatg gggctttccg ggagtgggaa atccacattg
     4201 atccgtctat tgaatcgtct gattgagcca acttcgggca gtatctatat tgatggagaa
     4261 gatgtatcga agttaagcaa agaagaattg cgagaagtcc gtcgtcataa aatcaatatg
     4321 gttttccaaa acttcggtct gtttccacat cgaacgatct tggaaaatac agaatacggg
     4381 ttagaagtcc gtggtgtacc taaagaagaa cgacaagaaa aagcagaaaa agcattagaa
     4441 aattctagtt tgctttcatt taaagaccaa tatcctagtc agttatctgg tgggatgcaa
     4501 caacgggttg gcctagccag agctttagca aatgatcctg agatcttgct gatggacgaa
     4561 gcgttctcgg ctcttgatcc gctgatccgg cgagaaatgc aagacgaatt actggacctg
     4621 caagccaatg ttcaaaaaac gattattttc attacccatg atttgaatga agctttacgg
     4681 attggggaca gaatcgcctt gatgaaagac ggcgaaatca tgcagatcgg aacaggtgaa
     4741 gaaatattga ccaatccagc aaatgattat gtacgtgaat ttgtggaaga agtcgatcgt
     4801 tccaaagtgc ttacggctca aaacatcatg gtacctgcat tgacgacgaa tatcgaatct
     4861 gatggaccaa acgttgcatt gacaaggatg cgtaatgaag aagtcagcat gctgatggct
     4921 gttgacagaa agagacacct gaaagggatc atcactgcag atcaagcgtt agaagctagg
     4981 aagcaaaaac gtccattgat cgacttccta gatgaaaatg tgacggtgat tgggaaagac
     5041 atggtggtaa gtgatatttt caatattatt tatgactcac cgactccact tgcagttgta
     5101 gaggatggaa aattaaaagg tgtcgtcatc cgaggtagtg tgattgaagc attagcagaa
     5161 acaagcgagg tgagtgaaca tgaataatct attgaactat caattaccag tggcaaactg
     5221 ggtagaaaat atcacagaat ggtttacgac aactttttct ggcctatttt catttttaca
     5281 aacgattgga caggcagtga tgtcggggat tacaaattta ctgttagtga ttccagctcc
     5341 tttgtttatc ttgttattga cggtagcagc tttctttatt tctaagaaac gtccaggact
     5401 tacgttgttc acattaatcg gtctatggtt tatttataac caaggtctat ggaatgattt
     5461 gatgaatact gtcacacttg ttcttttatc tagtgtgatc tcgatcatca ttggggtacc
     5521 attagggatc ttgatggcaa aaagcagtaa agcccaaagt attatcaaac cgatccttga
     5581 ctttatgcag acaatgccag gattcgttta tttgattcca gcagttgcat ttttcggtat
     5641 cgggatggtt ccaggggtat ttgcctcagt catctttgct ctgccaccaa ccgttcgttt
     5701 tacgaattta gggattcgac aagttcctaa agaattagta gaagcttctg attcattcgg
     5761 gagcacaagt cgtcaaaaac tattcaaact agaattgcct ttagctaaaa gtacgatcat
     5821 ggcagggatc aatcaaacaa caatgctgtc actttcaatg gtcgttattg cctctatgat
     5881 cggtgcaccc ggcttaggac gcggcgtatt atcagcattg caaagagcac aagtcgggaa
     5941 tggatttgtc aacggggttg cattagttat cttagcaatc atcattgacc gttttacaca
     6001 gcatttgaac cagccaaaca ataaaaaagc tgcaggagca acaacacaga aaagtaaaaa
     6061 acgtcaagga ctgatcattg gtgctgtagt agtagtgatc ttaggtgcga ttggtatagg
     6121 gagtttttca tctgccaaag aaacaaaacg tatcaactta tcttatgttg aatgggatac
     6181 agaagttgct tcaacaaatg tagtcggtga agtattgaaa caaatgggct atgatgtaac
     6241 gatgacgcca ttagataatt caatcatgtg gaaatctgtt tcaaatggcg agtcagatgc
     6301 catggtttca gcttggctac ctaaaactca tggttcacaa tatgcacaat ataaagatca
     6361 agtcgaagac ctaggtgcca atttgacagg agcaaaagtt ggtttagcag tgccagccta
     6421 catggatgta aattcgatcg atgagttaac cgatcaggca ggtaagaaaa tcataggaat
     6481 cgagccagga gctggtgtag ttacggctgc tgaaaacacg atccaaaaat atgacaactt
     6541 gaaagactgg aaagtagaga catcttcttc aggagcaatg accgttgcac taggtcaagc
     6601 gatcaaaaaa cacgaaccaa ttgtagttac aggatggaca cctcattgga tgtttgccaa
     6661 atatgatctg aaatacttag aagatcctga aaacggaatg ggaagcgaag agcagatcca
     6721 tacgatggtt cgaaaaggat tgaaagaaga ccagccagaa gcttacaaag tattagacaa
     6781 tttccattgg tcagaaaaag atatggaaaa agttatgttg gaaatcaaca atggaaaaga
     6841 tccacagcaa gcagcaaaag actggatcaa agagaaccaa gaattagtcg aaagctggaa
     6901 aaaataaaaa gacagacacg aaataaatgg agcttccaaa attggatcaa atatccaaat
     6961 ttggaagctc catgttcgtc tctatctttt ttgtctataa aaaaagagaa tgcgtagctc
     7021 atcttcgaaa cgttcaaaga cgccgacaaa ctattatgaa tttatgggtg cgagtcaaag
     7081 agatcgatcg taggtatgaa agactaatgt gattaaagtt tattatgtac gaagtgcatt
     7141 ctcaaaaaat attaaagaat atacatagtt tatatactta aaagtgatta aattcttatt
     7201 caggaattat tttgctacgt ttcaagaagg aattgttttt tgaaatttaa tgaatgttag
     7261 aataatagtc taaaaacatt aatttagggg tgattagatt atgaaacatt taaaaatttt
     7321 gtctattaaa gagacacaac ttatctatgg gggtaccact catagtggaa aatattatgg
     7381 aaatggagtg tattgcacta aaaataaatg tacggtcgat tgggccaagg caactacttg
     7441 tattgcagga atgtctatag gtggtttttt aggtggagca attccaggga agtgctaaaa
     7501 tgaaaaaaaa tgctaagcaa attgttcatg aattatataa tgatatatct ataagtaaag
     7561 atcctaaata ttctgatatt cttgaggttt tacaaaag
//
LOCUS       DANKKG010000122         7568 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000122 DANKKG010000000
VERSION     DANKKG010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.122"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(733..1911)
                     /locus_tag="PW004_002286"
                     /pseudo
     CDS             complement(733..1911)
                     /locus_tag="PW004_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
     gene            1941..2351
                     /locus_tag="PW004_002287"
     CDS             1941..2351
                     /locus_tag="PW004_002287"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727238.1"
                     /translation="MVTLFYRRDFLFSIYTKYFTLSSNIKTSTKLICKLFKLDKISNI
                     DDVKSKILTRKVSEPVKLTAQDKKNGIVLYGDDAKYNYNWDTSSEAEVLSIPTTDTSG
                     EKVTFKINFITAISKLEYGKQKKRDNNDQYLPKK"
     gene            2432..3052
                     /locus_tag="PW004_002288"
     CDS             2432..3052
                     /locus_tag="PW004_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727239.1"
                     /translation="MNINRVIKLIGAKNINKVRSEFIFPEDFFNWFFYQFSECKRLLG
                     ENYYLKGIGGFMGNVADEQNIIKGISEQTSELTVTKAFISNGESLKNVTARIRKENEV
                     DIVFGIDHECNSVIYVTQSANLILFENGCKDDFMVVYLYDILIPKLRSLYMSSESIFV
                     SEQKGSFSKKIGIEVIESIMCHNHITLEELEILIGQKNNNTKIQVS"
     gene            3753..4037
                     /locus_tag="PW004_002289"
     CDS             3753..4037
                     /locus_tag="PW004_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727240.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            4040..4957
                     /locus_tag="PW004_002290"
     CDS             4040..4957
                     /locus_tag="PW004_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2727241.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            4950..5732
                     /locus_tag="PW004_002291"
     CDS             4950..5732
                     /locus_tag="PW004_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2727242.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            5733..6413
                     /locus_tag="PW004_002292"
     CDS             5733..6413
                     /locus_tag="PW004_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2727243.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            6406..7302
                     /locus_tag="PW004_002293"
     CDS             6406..7302
                     /locus_tag="PW004_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL2727244.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
BASE COUNT         2416 a         1236 c         1530 g         2386 t
ORIGIN      
        1 aatgaaaaac gagaaggaag atgactttaa agtagtttag tatgaaggtc gattgataaa
       61 acgatgtgga ttaatgctaa ataatgagta atttttcaat aatacaaaaa ctgtgtacag
      121 tattagaagt agagaaacgc tagcgtcgaa aaaaagatat ttttcgtgaa aagtaatagg
      181 ggctaaaaag aaagtataca atttatttat gtttctaagc atataaaaaa gagcgttgaa
      241 aggaatattt ccccttcaac gcttcttttt tagaagattt taatgaccat ctcaacttca
      301 ttttcaggtt taggtaattc atggaagctt gtgtttatcg tgcttaattg atcaactaac
      361 catgttttac tttttgactc gtagacaaat ctaaaattaa tatcattgtt ctcgtgttga
      421 agaggtttct ccaaataatc tggtgaatat tcatagtcaa cgtaacctgc taatacaaaa
      481 gaaagcagtc cattatttag tgtcagattt aacggatggt cgtaagcttt tattttttta
      541 atgtcatatt gtacatctta tgaagactta atgactactt gtagtcaata gatttaaagt
      601 atttattttc tcatttatgt atcaagaata aatactgata taataaaaat tatatcaatg
      661 ttttgatgaa gtaatttgga gggagcgtca ataattttgt gtaaataact cgtccttctg
      721 caaaataatg ggttactcag taaacattga agctaatgta tccgtcactt gttggaagcc
      781 tttatggctt ctatttaaga atttttgatt gtatgtatca aagatggata ctaggaaacg
      841 ctctagtgat tcttcatttt gaaactgctc ttttctgcgg ctgtatttct tgatttgttt
      901 cttgaaagat tcgattaaat tagttgagta aatgcttcgg cggataccag gcggaaactc
      961 ataaaaagtc aataaatctt gattttttat cagtgactgc gtcactttag gataagtttt
     1021 ttgctacttc tcaatcatac tccctaaaaa ggtatttgcc tcttcctttg agctagcttg
     1081 ataaacagcc ttaaaatcat cacagatttc ttttcgatct ttgacacgta ctttatgagc
     1141 aatattacgg gaaacgtgaa cacagcaatg ttggtattta gctttaggat aaatctggtg
     1201 aatcgtatct ttcatgcctt ttaagccatc ggtaataaac agtaagacat catgaacccc
     1261 tctggagtaa atgtcctgta acaactcatt ccaaacgtag gtcgattcag tcggagcaat
     1321 cgcataactt agtacttctt tagtgccgtc ttctcgtata ccaatggcaa tatagattgc
     1381 ttctttggag acggtttgcc gttttaaagg aatataagta gcatccatga aaatagcgac
     1441 atacttatca tttagagttc taactttaaa tgcatttact tcttcagtca gcactttagt
     1501 catgttggac atggtttggg gcgtgtagtg atgcccatac attttctcaa tcaagtctgc
     1561 gatttccgac atggtaatac cttttcggaa gaggtgaatg acggtctcct ctaagatatc
     1621 attcgtccgt ctataagcag gaacagtctg ttgcttgaac tcgccgttgc ggtcgcgcgg
     1681 aatctggaga tgaagttccc cgtactcggt cttgaccgta cggtcatagg agccgttacg
     1741 agaattcccc gtgttaaaac caatgcgatc gtacttttcg taatcgagga aagccgttaa
     1801 ctccgttttg agaagcgtat tgacagcttt ttccaaatgg acacggaata attcattcaa
     1861 atcgcctttg ttcgctagag tctttagaat ttctgtagta aaatcgttca tatggaagtc
     1921 ctcttttctg tgaatgtgtt gtggtaactc tattctacag aagggacttc cttttttcta
     1981 tttacacaaa atattttaca ctctcgtcaa atataaaaac tagtacgaaa ttaatttgta
     2041 aattatttaa gcttgataaa ataagcaata tcgatgatgt aaagtccaag atattaacaa
     2101 gaaaagtaag cgaaccagta aagctaacgg cacaagataa aaaaaatgga attgttcttt
     2161 atggtgatga tgcaaagtat aactataatt gggatacctc ctcagaagct gaggtattaa
     2221 gtattcctac taccgatact tcaggggaaa aagtcacctt taaaataaat tttataacgg
     2281 caatttctaa gttggagtat gggaagcaaa aaaagagaga taacaatgat caatatctac
     2341 ctaaaaaata acgaattaat gatgcatatg ttaaagttgt ttttttgaat taaacaactc
     2401 aatttattca gttatatgtt catcgcagca agtgaacatt aatcgagtta taaaattaat
     2461 aggagctaaa aatataaata aagttcgatc agaatttatt tttccagaag atttttttaa
     2521 ctggtttttt tatcaattca gtgaatgcaa gcgactctta ggtgagaatt attacttaaa
     2581 aggaatcggc ggatttatgg ggaatgtggc tgatgaacaa aatattataa aaggaatatc
     2641 tgaacaaaca tcagagctta cagtaactaa agcatttata agtaatggtg aaagtttgaa
     2701 aaatgtaact gcgcgaatta gaaaagaaaa tgaagtagac attgtttttg gaattgacca
     2761 tgaatgtaat tcagtgatat atgtaacaca atcagcaaat ttaattcttt ttgagaatgg
     2821 ttgcaaggac gattttatgg tggtctattt gtacgatata ctaattccaa aacttcgttc
     2881 cttatatatg tcttctgaaa gtatttttgt gagtgaacaa aagggatctt tctcgaaaaa
     2941 aataggtatt gaagtaattg aatcgattat gtgtcataat catattacgc tagaagaatt
     3001 agaaattttg atagggcaaa aaaataataa cacgaaaata caagtttctt agaaatcatt
     3061 ttttattgag atatctagat aaatgattta ttcttcaatc aaccaactcg ccaagctgag
     3121 gtaatgtttt gaggtaatga taacatacaa ctttttatac cctagtaaaa ctctataaac
     3181 aaagacttat taatttcgca tgaaatacat tcctgtaaaa tgcaataaaa tagagttttg
     3241 tttaaccagg tcttaagaaa gcccgtaaag cttcacagtt ctcaaaacgt taattgcgat
     3301 tatcgttcga gttcacgagt tacgagaatc gtactgggcg aagcaatgct gagcgcagtt
     3361 accttaatcc taacttttct ggcactttcc acaatttgga aagtgccttt tttgtgtttt
     3421 tagctatgtt aataggtagg attcttggtc tataatatca ttcgatctat aaacaaacgg
     3481 gaaaaagata aagtatcatc aaacaatatt atttatccgc ttctgaggtt gtgacagaag
     3541 tggtcagctt caagaaataa gaaggaattt acaaaaattg cttctcatgt ttgataaata
     3601 ttgtgacttg tgaccgagga gttggcagga gaacaccgtt tattcggaag aagggattgt
     3661 gaaaagtctt ttgtcataac ctcttatttg gtagctaatt gtaaatccca aacggcatgt
     3721 attatttttc ataagtccgt ttttaaggat taatgttttc ttttgctttt tatatggtct
     3781 ggcttcagcg agaagttcca acgcatatac aaggacgtct gatgtcaacg ataacaacga
     3841 ttaccatggt ggccagtcca cttggattag cactgtttgg tgcgctcttt gattggcaga
     3901 caactcaacc acgtctgcga gacagtctga tttttcttga aattcaattg gaaaatttac
     3961 actttcttta tacatttctg gctgctttct ttatacccgt caactatact tcacttatca
     4021 ataagaaaga aggataagta tggattttgg aaatgaaacg gtcttgaaag caactggtat
     4081 aaccaaaaaa tacggtgctg ccaaagcatt agataaagta tcgatcgaaa tcaaacgtgg
     4141 aatgatctat ggtttgattg gggagaatgg agcgggtaag tctaccttta tgagaactat
     4201 tatgggtttg atctcgattg atgaaggttc gatcgaatta tttggaacga cggatttgca
     4261 ggcagcaaga cgacgtatgg gtcaatcgat tgaaacccct gctctttatc cagaattaac
     4321 tgcaagagat aacctacgta tccaagcagc gaacggcggt gtgtcggata gagaaatcga
     4381 agacctgctg aaaatgatgc gattggaaaa tacagggaaa aagaaagcaa agaatttctc
     4441 tttaggtatg cgccagcgtt tagctattgc aaatgcactg atcacgaatc cagaattttt
     4501 gattttggat gaaccgacga atgggatgga tccagcaggg atggcagaaa tgcgagaaat
     4561 catccaacgt ttggtaaaag aacggggcat caccgtcttg ctttctagcc acttgctaga
     4621 tgaattgtca cagatcgcca ctcactacgg tattttgcat gaaggacact tgatcaaaga
     4681 attatcgaaa gaagaattgg cacaagaatc tcgtcaattt ataaaaattg acacttcggc
     4741 gacggaacaa gcagtaacgg ttctcgactc tcttggttat agggattatt ttgtccaatc
     4801 tagccgtgtt atccagcttt ttgaaggaat tgaccaagta gcagccatca atcaagcatt
     4861 ggtagaggca aaagttcctg tggatggtat ccacctagtt gggcaaaaac tggaagatta
     4921 tttcttacaa ttaactggag gcaatccaca tgtttaatct attaactgct gaaaaaataa
     4981 aagtatggaa gagccgtaaa atgtgggtct cactaggact gatggtcgta cttccgcttt
     5041 tttattcttg gaacgaatgg tatatgcata caaaatacgg caatgaatta aaccaagcaa
     5101 cggatacagt catcaacggt gcaacaggga ttttaatggt ggaaaaaatg gctgcatgga
     5161 ttcttctggc tttcgcagca tttgcgtgct tttatattgg ggaagaattt caaaacggta
     5221 cgattcgcaa tacgttatct ttaggacgga atcgaatgac ttattatgtg tcgaaacttc
     5281 tcgttacgct tttgatcaca acacttggcg tggttttgat tacggggcta gccatgattg
     5341 cttacacact tgctttcgga tttggtgaag ttgagggaat caaaaactat ggaaattatg
     5401 ttctaaaagt atttcctgta ttgcttctgc tgatattagc gacattatct gttccagtgg
     5461 ctttgacatt catcactaga agtaccagtg tcagtttgct tctttctttt ctttatatca
     5521 tgggaacggc cttcgttccc ggtgttttcg caaaaatcaa aggcttggaa tttctaacgg
     5581 agtggtttac ggaaacgtgg ctcatgtata cggactttgc gcaacaagca acttattcgc
     5641 aagcaccgaa aatgattctg gtcagtctag taacaatcgt ggtatcatca gctctaggaa
     5701 tgtttatttt ccaaaaatct gacattaagt aggtgttcct gctggaaact attttgatta
     5761 tcgaagacga tgaagctgtt cattctttac tggaggaggt tcttgaacag agatacaaac
     5821 ttcttgatgc ttattcaggt acggaaggaa aattattgtt aagcacatat cctgtcgatt
     5881 tgatccttct agatctcatg cttccagggc tttcaggtga agcattgtta gcagaaatcc
     5941 gacagacatc gaatgttccg atcatcgttc tttcagcaaa aagcgatcaa agagataaag
     6001 tttccttgtt ggcagcagga gcggatgact atgtgacgaa accatttgat atcgaagaac
     6061 tgttgttacg aatcggcatc caacttcgtc atcagacttc tgtccaagta aaagatctgt
     6121 cgcaacgcat ctattacaag gaaatcttac tggacaaaga gtcgagggat gtaaatgtga
     6181 atggtgtggc tgtccatttg acaggaagag aattcgatct gctgaagctt tttttagaac
     6241 atccgaaaaa agtattttcg cgagcaaatc tttatgaaac agtttggcaa gaaccatatt
     6301 ttgatagtga gaaaacagtg aatatgcata tttccaatct tcgagggaaa ctagcaaagg
     6361 cacatgctgt gtatattcat acggtgtggg gagtggggtt tagatttgat taagtggctg
     6421 ttgcttctcg ttgttatctt tatgggaagt tttattttct tcctgcaaag ggaaatcaag
     6481 agattaacta agaaaatcgc cactctttct gaacgacaag aatatggtgg tcgtctttca
     6541 gtcgaatttc gagaaaagaa tctaatggac ctagtagatg cactgaatac atgggtggat
     6601 gaaaatgaac aaaaaggaca agcatttcaa gaaatggaag aaaacatcag actatcgatc
     6661 gtcggccttt ctcatgatct cagaactcct ttgactgcta ttaacggtta tgtacaatta
     6721 ctggaacagg tagaggatac ggagaaacgg gagaagtatt taacgatcat caaacaatct
     6781 gttgcaagat tactcagtat gacggatcag ttttatgatc ttgctcgaat agagacgaag
     6841 caaaaagaaa tggatctcca tacgctttct ttttctaaag aggtggaaga agtatttttc
     6901 tctttttatg aacaattttc tgagaaaaat atcgaggtag cgttcgctga acactcgata
     6961 aatacaactg tattagcaga tccgctcatg ttgctgcgag tgctgcaaaa tatcatacag
     7021 aatttattgc gttatgcaga tggacaagca aaggtgacat tttcaaaaga ggattcattc
     7081 attcgattaa cagtggccaa tcctatcaaa ccaggagcgc aaacctctgt tgaaaaagtc
     7141 ttccagcgtt tttataccga aaataccagc cgcacaaata cggaatctag cggaatcggg
     7201 ctttacctgt caaaaaaatt ggtggaagga atgggaggag agatgaccgc tagattagac
     7261 gggggcatct tctccatctc cgtcaagttg cggagggttt aattggctct aatttacctg
     7321 aagtgaatga caaaaaattg tatctcgttt taaagacatc ttcttttttg gaaggtgttt
     7381 ttattttggt gtgattgata cgaattaggt aacgaagaca gttatttttg tacccccttt
     7441 tcaaatagcg caacagtgct atactaaaac taacaaatca aggaacggag agggatatgg
     7501 gccaaagaga atttatcgtt ttaattatta ttgcagttgt tattttatta gttgttttag
     7561 acattttt
//
LOCUS       DANKKG010000123         7541 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000123 DANKKG010000000
VERSION     DANKKG010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7541
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.123"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(883..1296)
                     /locus_tag="PW004_002294"
     CDS             complement(883..1296)
                     /locus_tag="PW004_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HDL2727245.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1752..2237)
                     /locus_tag="PW004_002295"
     CDS             complement(1752..2237)
                     /locus_tag="PW004_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727246.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2376..2591)
                     /locus_tag="PW004_002296"
     CDS             complement(2376..2591)
                     /locus_tag="PW004_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HDL2727247.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2815..3615
                     /locus_tag="PW004_002297"
     CDS             2815..3615
                     /locus_tag="PW004_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL2727248.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3634..5502
                     /locus_tag="PW004_002298"
     CDS             3634..5502
                     /locus_tag="PW004_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HDL2727249.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5495..5986
                     /locus_tag="PW004_002299"
     CDS             5495..5986
                     /locus_tag="PW004_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HDL2727250.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            5974..6528
                     /gene="srlA"
                     /locus_tag="PW004_002300"
     CDS             5974..6528
                     /gene="srlA"
                     /locus_tag="PW004_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HDL2727251.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6546..7541
                     /locus_tag="PW004_002301"
     CDS             6546..7541
                     /locus_tag="PW004_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HDL2727252.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2685 a         1210 c         1433 g         2213 t
ORIGIN      
        1 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
       61 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      121 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      181 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      241 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      301 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      361 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      421 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      481 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      541 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      601 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      661 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      721 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      781 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      841 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      901 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
      961 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1021 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1081 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1141 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1201 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1261 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1321 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg
     1381 ttagctttgt gaatatttca tctaatacga tacaaaggct atctttctaa tttgaaaaag
     1441 ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc
     1501 caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt aactcagttg
     1561 accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa gtgagtatat
     1621 tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat ttatactccg
     1681 tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta aatccgtata
     1741 atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga tccttcgggt
     1801 gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg tttgctggaa
     1861 gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat atcgtcactt
     1921 tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt ttctaatgga
     1981 agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa cgactgaata
     2041 cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat ggaagtagta
     2101 gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca
     2161 gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa caagcctgcg
     2221 taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc agtatatcac
     2281 ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa gggaaggttc
     2341 atgaagataa taactttttt aataattata gctttctaat atctttcatc aacttttcta
     2401 gtaatcattg ggaaaaattt caagcagtag atcgcacaaa tacctacgac gatataaatg
     2461 atcttcgcaa aaatagttgt tgaaccacca gcaatcatag ctactaaatc aagttcgaac
     2521 aagcctacca acagccagtt caaccctccc acaatcaata aagttaatgc aatagcatct
     2581 aaagttttca ttagaattct cctttcttaa atgataaaac aattatatat caaaaagaga
     2641 aacattgctt atataacgct caggaatttt agcaatctat gcaaaaagat gaaaattttt
     2701 tttgctcttc cattagtaca cattttttag ctgaagctgt aaatttcatg tcttaatttt
     2761 ttttagtgag cttttatact ggatttgtaa gaaaaaagaa ggagaggatc acatatgtcg
     2821 gattggttgg atattgcagg aaaagtagta attgtaactg gaggttcctc aggaattgga
     2881 agaagtatcg tcgaaaattt gttgaagcag aatgcacaag tagcgaattt tgatgtaact
     2941 gaatgccgta tacagcatga aaacctacta tctttaaaag tcgatgtgtc ttctaaaacg
     3001 gacatagaag aagggatata taaagtaatg aaacatttca agacgatcga tggtctagtc
     3061 aataatgcag gaataaatat tccttctctt ctaatagata gaaatcatcc aaaaagcaag
     3121 tatgaattat cagaacaagt ttttgataag atgatcgctg tcaatcaaaa aagtgtatat
     3181 ttgatgagcc aagcagttgg aagaatcttg gtgcaaaagg gatcaggagt gatcgtgaat
     3241 ctttcttctg aatcaggatt ggaaggatca gaaggacaga gctgttatgc tgctacaaaa
     3301 gcagcgatga acagttttac tcgttcttgg gcaaaagagt taggaaagag gaatgttcgt
     3361 gtggtaggtg ttgcaccagg gatactggaa gaaaccggtt tgagaacaca agaatatgaa
     3421 gaagctcttt cttatacgag aggtataagc gtcgaacagc tgagaaacgg ttacagtcga
     3481 acaagtacga tccctctagg aaggagtggt aagctgcaag aagtggcgga tctagtctgc
     3541 tattatcttt cagaaagatc aagttatatc actggagtca cgactaatat ttcaggaggt
     3601 aaaacaagag gataaaggat aggggggacg cacgtgacgc tagtaaacag atggtaccaa
     3661 ttattgaaat tactagtcac tcacaagaaa gtttcaatcc aggaagtgca aaaagaaatc
     3721 aaaaccagta atcaaaccat aaaaaaagat attggggagt tgaatgaaga aattgccggt
     3781 attgcaaaaa tcatccaaaa aaacaaacat tttgaacttg aaatttatga ttttgatttt
     3841 tttgatgaga tcatgcaagg aaaattgaaa acagccagtg atttcaattc ggcatccaaa
     3901 agaatcgctt atttactaaa taggctgatc gaaacagagg atttcttacg tatggatgat
     3961 ttgtctgaag aagtcggtgt cagcagagga acagtggtca aggatctaaa gacattaaaa
     4021 gagatgatca atgattttga tgtaaagatt acaggcacac caaataaagg actaagaata
     4081 ataggagatg agctagaatt acgactactt ttattttatt ttgtttctcc ctacttttca
     4141 gagacgaaaa cagaacagtt tctagaagaa aaacaagcaa tcaaacattt tcagaagaag
     4201 acgaatgcta gtaaacaaga aatcaccctt ttaacgaaag ttatcggtat ttctttgaat
     4261 agaatctcta gtaactatct gttgaagaag cccatcaatc gttacagtgg gtgttttgaa
     4321 tatatccaag aattcaatga actgatcaat cagttggaag aaatctatga actaactttg
     4381 agccaatatg aaaaagattt tatcagtttt cctttgaaca tcatcaacac aagtataacg
     4441 atgataagga aagctaactc tgatttgcgc ttatatttcg accccatgat gatgcgtatt
     4501 cgacgattag atctcattga tctggatgag tccttcttat atcaagagat gaaacagcac
     4561 ctctctttat tgataaatcg ggtaattttt cgttatcgat tgactgattt attttatggt
     4621 gagatagaaa aaaagtatcc tttttcttac caaatcgcta gcgaatgcat acaagcatta
     4681 gaagctatct tgcaacataa gatctctaaa gtagagatca gctacctaac gttgtatttg
     4741 gagttagcat ttcgatatca aaaagagcat ttttcctcta aaaaagtagc cattgttcat
     4801 acgacaggaa aaggaacagc attaatgatc caaagacaaa tcaaaaggat attggggagg
     4861 gatatacaaa taacacatgt ttcagaagaa caatatgaaa aagtagatct aaacaaatat
     4921 ttcgctgttt ttacaacgat tccgttaaaa aatgtaaatg aagagacacc ggtgatccat
     4981 ttgactaatc tttttgatga ctcttggtta agggcacaat ggcaaaaagc aaaaaaaaag
     5041 cgattagccg ggtttcattc tactgttttt ttctatcaaa tatggaaaga aaaaaagacg
     5101 tatcaagaat atttaaacaa attagtcgct cctttgatca gaggctatta tgtggatgaa
     5161 gaatttgaaa aaaggatctt gaaaagagaa tatcagcaaa gcacatttgt taacaaagaa
     5221 tttgcttttc ctcacacaac caaccagcta tctcaacaca ttgttttatc agtagggcta
     5281 ataactgatc aaggtttagc agaaacagga ggaaatggag ttattttttt atttgccata
     5341 cctgaagaaa tgacggctga agttgagaat gaattatttg agctatacga tatcctattt
     5401 gaactcataa aaatgggaat agatgagttt attgaattag caccgacaca agaatcattt
     5461 ttaaaactaa tcaaaagtaa aggagagaga cttcatgaat gatatgtatc tattcatagt
     5521 tttcgcagct tcagcttatg tgctgcaagc agttatcagt tggaagcaac taaagcattt
     5581 caatcaaaca tatagcgaac tgcgaagaaa aggaaaagta gcgattggta aacgacctgg
     5641 gaaaatccgt tcaggcacga ttgttatgct agctatagac gatcatgatc aagttttaga
     5701 agtcagaaaa atgcaagggg ttacatcttt ggcaaaattt cattcattac ctgtctatgc
     5761 gggtgaagat atccattaca tcgatcgtta ccatccctca gttcgagcgg aagatcgttt
     5821 gacacaagca gctttagaaa atgccagaga agtatactta cgaatagagg caggaaatga
     5881 aaaaagtgaa caaccttcat tgccttttat tgctatgctc aataattttc ggatatggaa
     5941 aagtacgatc aaagaaaaaa ggagtgacaa acgatggata tcataacaaa atttgctgag
     6001 ggatttatga ggctgttcca aacaggtgca gaaacattca taagctggat gacaggaatc
     6061 gtaccagttg tcttgatgct gatggttgca atgaatacgc tgattgcttt tttaggtgaa
     6121 gaccgagtaa cgaaagtagc caaattatca gcgaaaaatc cgcttactcg ttaccttgtg
     6181 ttaccgttct tatctgcttt tatgttggga aatcctatgt catttacaat ggctcgtttc
     6241 cttccagaat actacaaacc aagctattat gctgctcaag ctcaattttg tcatacgagc
     6301 aatggtgtat tcccccatat taatcctgga gaattgtttg tatggatggg gattgctcaa
     6361 gggatcgaaa ccttagggct aaattcgatg gagctggcta tccgatactt gcttgtggga
     6421 ctgctgatga actttattgg tggttggata actgatttta ctactggatt tgtatgcaga
     6481 cagcaaggaa tcgttcttag caaaaaagta gagctttcag ttgattagag aaaggagagt
     6541 tttaaatgta tggtgatatt aagatcgtaa gaggaaacgg cgggtatgga ggtcctttga
     6601 ttattaggcc gacaaaagat aaacacaagt tcatctatgt cacaggtgga ggtgagaggc
     6661 caatgatcgt agataaaata gaagaactga caggtatgga gtctatcaat gggttcaaaa
     6721 catcaatacc tgatagtgaa atcgcgcttg caatcgttga ctgtggaggg actcttagat
     6781 gtggaattta tcctaaaaaa gggataccaa ccatcaatat cgtttccaca ggaaaaagtg
     6841 gcccattagc acaatatatc actgaagata tttatgtttc agcagtaggg ttagaacaaa
     6901 tacagccact aatggaaact gatgataaca aagaaacagt aaatactgca tctccagata
     6961 aaaagtacaa atatgatact tctaaaaaga taaccgagca aaaagcagag cagcaaaact
     7021 ggatagcaaa aattggaatg ggcgcgggaa aagtagttgc tacattcaat caatcagcaa
     7081 gagaagcagt agatacgatg atccggacag tgatcccttt tatggctttc gtttcccttt
     7141 tgattggtgt gatacaaggt tctgggatcg gtaatgtatt tgctaacatt atggcacctt
     7201 tagcaggaaa tgcagttgga ttaacaatca ttggctttat ttgttcactg ccatttttat
     7261 ctcctttgct tggtccaggg gcagttatta gccaagtgat tggaacattg atcggtgtag
     7321 agattgggaa aggaaatatt cctccccaat tagcattacc ggcactattt gctatcaata
     7381 ctcaaaatgc atgtgatttt attccagtcg gtctaggttt ggaagaagca aaagctgaga
     7441 cagtagaagt aggtgtacca tcagttcttt attcaagatt tcttaacggc atcccacgag
     7501 ttatcattgc ttgggtagca agttttggat tatatagctg a
//
LOCUS       DANKKG010000124         7390 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000124 DANKKG010000000
VERSION     DANKKG010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.124"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(204..1652)
                     /gene="cls"
                     /locus_tag="PW004_002302"
     CDS             complement(204..1652)
                     /gene="cls"
                     /locus_tag="PW004_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDL2727253.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGEIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            complement(1804..2697)
                     /locus_tag="PW004_002303"
     CDS             complement(1804..2697)
                     /locus_tag="PW004_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2727254.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            complement(2681..3367)
                     /gene="ftsE"
                     /locus_tag="PW004_002304"
     CDS             complement(2681..3367)
                     /gene="ftsE"
                     /locus_tag="PW004_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HDL2727255.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            complement(3472..4573)
                     /gene="prfB"
                     /locus_tag="PW004_002305"
     CDS             complement(join(3472..4500,4502..4573))
                     /gene="prfB"
                     /locus_tag="PW004_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HDL2727256.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            complement(4699..7233)
                     /gene="secA"
                     /locus_tag="PW004_002306"
     CDS             complement(4699..7233)
                     /gene="secA"
                     /locus_tag="PW004_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDL2727257.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
BASE COUNT         1975 a         1639 c         1289 g         2487 t
ORIGIN      
        1 gctatactgg ctttgaaatg tgtctctttt aatagcatat attagcttat ttcttgcttt
       61 ccccaaaaaa caagagatac cccaaagtta atttcgctca tgcgagacgc tttctccatt
      121 tctgacgtac tctcagaaat ggccccgtta aaaaaggaca cgagatctcc atctgttgat
      181 ggttctcatg tcctttttat gttttaaagg agcggcgaaa tcaatcttgc taatccttct
      241 ttcatcttga tgattattcc tcggttttca tacatttctt ttgtgagatg cgtagaaatt
      301 ttcgagtctt caaaaaaagc ttttcttaca cgtgaagcca ttcgttcatc ataaaccagt
      361 gtattgacct caaagtccaa gcgaaatgaa cgaacatcga tatttgctga gccaactgaa
      421 acaatgccac catcgataat catcgtcttt gcatgaataa atccattctc atatgtttcg
      481 atccttgctc catactcgat cagctctgca gcgaaagaat acgttgccca gtaaacgaga
      541 agatgatctg gtttgttagg tatttggata tgaactttta cgcctgaaag aagagccaac
      601 ttcagtgctt catgaatcga tccatccggt atgtaataag gcgtttggat caatatctcc
      661 cgtttggcta acgaaatcat tttcaaataa gtcattttga tttgttcatg ttcggtatct
      721 ggtccgcttg tcacgatttg aacagctata tcaccagtag tgtgaatgga cggaaaaaat
      781 ttgggagaat agcctacttc ttttgtatgc tgagaattcc agtccatcaa aaaacggttt
      841 tgcaaggtat acaccgcttc tccgtaaata cgcaaatgat tgtctcgcca ataaccaaat
      901 ttctttacca atcctagata ttcatttcca acattgaacc cacctgtata tccgatttcc
      961 ccatcaataa ctacgatttt acgatgattg cgataattga tccgaggatt aatatacgga
     1021 acaaatagag gaaagaaaaa tgcgacttct ccgcctgctt ttttcaaatt ctgaaaaaag
     1081 ctggaagaaa cttgtgtgga accccatgca tcaagaagga ctctgacttt tacccctcgt
     1141 cttgccgcct ctgtcaatgc atctcgaact tcttcaccta atgcatcact gcgataaata
     1201 taatactgga agtggatatg gtcttctgcc tgcttgatat cttcaatcag cgcatcgaat
     1261 ttttcacgac catctgtaaa taaagtgatt tcatttttag tcgtatagag cgtactttca
     1321 aataccgtga gcatatagat caattgtttc acatctactt gtccagtagg cggatgagga
     1381 taaagccctc gcatcaatgc ctgtttgtct tcttcaattt ccaggttctt accgattttc
     1441 ccttggatct tcaaatcaaa gattttctct ttggaaatcc ctcttcctaa aaaaatatat
     1501 aaaataaatc ctacaactgg aatgaacatc aaaacgagca gccatgccca tgtttgagca
     1561 gtttcctttc tttctcgaaa aacgatgata aaggaaagca aaatattcat aatcaaaagg
     1621 atcgtaaaaa aattgtcaac gataattttc acggcttcac ctcttcttct accaacttaa
     1681 gaatacccaa gttgtttttt aaagtaaagg gaaaaaagcc gcttccttct attctcctag
     1741 ccaataaaaa aagaaagctg gaacgaaact gtccaacttt ctcattttat ttatattgtg
     1801 gtattagaat ttcaagaaac gtctcataga aatgatcgag ccaagtgaac caatgatcac
     1861 accaatgccg accattaaaa gattgatttt ccaaacgata ttttgtggat gaatcaatga
     1921 ataatgggaa cgtaataatg atggattgat caattggaaa aaccatgcat aaccaaatgt
     1981 gatgatcaaa attggaagaa ttgcaccgat cagaccgatc cagccacctt caaggaaaaa
     2041 cggccagcga atatatccat tttttgcacc gactaaacgc atgatttgaa tttcacgctg
     2101 tctagacaaa atcgtgatcc gaatcgtgtt cgaaatgaga aacatcgcta cgaacagcag
     2161 caatgccgct gcaactaatc cccatgtccg aactgcacca gcgatttgga aaatccgatc
     2221 tgaggaagct ccaccgtaat cagaacgaga aacattcttc aaatctgctg cttcttctgc
     2281 tacttgtttt gtataagaag gatcagtagc acttactaca tacacatcat ataacgggtt
     2341 attgtcacct tcaaacaatt tccaggaatc acccatttcg ttttggattt ttttcaattc
     2401 ctgttctttg cttgaaaatt caactttttt aacatgatct aagtcattca attctttttc
     2461 tagctttttc atctcttcgt cttttgtacc gatatctacg aatacagata catcaacgtt
     2521 tccttctata tcctgagcta atttagttgc attcatgata actgccatga atacaccgac
     2581 taaagtcaac gtgatcgtta ccgcactgac tgaagcaact gtcatccagc cgttacgttt
     2641 caaactcttg atactttcta ataaatggcg gaaaaaggtt ctaatcatcg tatccatatt
     2701 ccccttccgc ttgatctcga ataatgcggc cgttttcgat agcaattact cggtgccgaa
     2761 tcgtatttac gatcgtactg ttatgtgtag ccatcacgac agtagttcct tgggcattga
     2821 tccgatctaa taatttcatg atttcccatg agttttctgg atccagattt cctgtcggtt
     2881 catccgcgat aagtacttta ggcgtattca cgattgcccg agcaatagag acacgttgct
     2941 gttccccacc ggataattcg ctaggaaaga ctcgaacttt gtgtttcaaa ccaacaaggt
     3001 ccagcacttc cattactcgt tttttgatat ctcgaggttt tctgccgata acttgcatag
     3061 cgtacgcaac attttcatat actgtttttc ttggtaataa tttatagtct tggaacacca
     3121 cgccgatttc tctgcgtaga taaggaactt cgcggttttt gattgctatt agatcatgac
     3181 ctgcgacgtt caaagttcct tttgaagctt tttcttctcg atacatcaat ttgatgaatg
     3241 tagatttacc ggctccagac ggaccaacca catagacaaa ctctccttgt tcgatgacaa
     3301 tcgagagatt gcggatagcg gtcgtaccat ttgagtactt cttcattaca tccttcattt
     3361 caatcatggc gttctctcca tatctttttc agtctttagc tattatagca tggcaatgtt
     3421 tcaacatgct tgcaaaatct tacaatttta tttcaattta acaatgggtt cttattgtag
     3481 atgcattttt aaatagccgt caataaataa atcaaggtca ccgtccataa ctgcttggac
     3541 atttcctgtc tctacgtttg tccgatggtc ttttaccata gaatagggat ggaaaacata
     3601 agaacggatc tgtgaacccc agccgatttc tttttgttct cctcgaagtg cagctgcttc
     3661 ttgttctttc ttttccactt ctaattgata aagcttagct tttaacatcc ccatcgcttg
     3721 ctcacggttt ttcaattgtg agcgttgtgc ctgactagct actactgttc ctgtaggcaa
     3781 gtgagtgatc cgtacagcag attctgtttt attgatgtgc tgaccacctg caccacttgc
     3841 tcggtacgta tcgattttca aatcatctgg attgatttct atatcgatcg tttcatcaag
     3901 ctctggcatc acatctaccg aacaaaaaga agtatgacgt cgtttcgctg agtcaaaagg
     3961 cgaaatccgt acaaggcgat gaacaccttt ttctgatttt agataaccaa aagcgttatg
     4021 tcctttaatc agcagggtca cactcttgat ccctgcttca tcacctgctt gataatctag
     4081 tgtttctact tgatagccat gctgctctgc ccaacgtgta tacatacgta aaagcatgct
     4141 gccccagtct tgggactccg ttccgccagc ccctggatgc aattcgacga tcgcattgtt
     4201 tcgatcataa ggtccgtcta acagcataga taattcatac gtcttcattt tttcatctaa
     4261 tgcattgata cgtgtttctg cttcttcttg cattgctgca tcaggttctt cttgcagcat
     4321 ctccaccatc aattcgattt cttctagttc ttcggcaaga gaatggaatt ggtcataggt
     4381 ttctttatta gcgttatttt cgctgatcac tttttgtgcg gcttcgctgt catcccaaaa
     4441 accaggttca gccatttttg cttctgcttc cgcgatgtct tcttccagct gatctaagtc
     4501 aaagagacct cctgaaactt gtgacctttt cctgcatttc tgtcaggaga tttcgaattt
     4561 cggatatttc catgtttttt ccttctttca ctatactttt ttcattatat ctagtctaac
     4621 agaatcagca aataacagaa agaaggtgag acgaattctt aaaaaaagaa tttgtctcac
     4681 caatcctgct gtcagaaatt agtttgcatt ttttccatga cagttcttat attttttccc
     4741 gcttccacaa ggacaaggat catttcttcc gaccttattc acatggattg gctgtttttt
     4801 cgcgctagta ttactatttt ctttggcatc tccgtcttct gagtgagaag ctgttccttg
     4861 agaaacctgt tcacgctgta cgttttgacg gatctcagct ttcataaaga ttcgagtgac
     4921 ttcatactcg atggcgccta ccatatcttc aaacatctta tagccttctg tttgatattc
     4981 gactaatgga ttgttctgtc cataagcacg taagccgatt gactgacgta attgatccat
     5041 tgcatcaata tggtctgtcc atttagagtc cacaacacgc aagatcacga ctttttcaaa
     5101 ttccaacagt tgctctggac catttagttg agccgctttg gtattaaata cttcttgtgc
     5161 tcgttcgttt agatactctt tgatctcctg agctgttttt ccttcgatat ctgcaagagt
     5221 aatgctatct tcatggacaa gagtactgcc ggcaaaatcg atgatacttt ccaaattcca
     5281 attttctttc tctaattgtg tatgcgcatc tactacacga ctgattgtcc gtttgaccat
     5341 gttcattagt ggcttggata agcttttttc ttccatgatt acttcttgtc gctgtttata
     5401 gattacttca cgttgttcac gcatcacatc atcgtattgt aagacgtttt tacgtgtgtc
     5461 atagttattt ccttctacac gtttttgtgc agattctact tgtttcgtca gcatcttgct
     5521 ttggataaca gcatcttcct caccgatctt catccgatct aagaaagctt tgattcgttc
     5581 tgaaccgaat cgtttcatca aatcatcttc caatgacaag tagaactgtg acataccagg
     5641 gtctccttga cgtcctgcac gtcctcgaag ctggttatcg atacgacgag attcatgtcg
     5701 ttctgtcccg ataactgcta gaccaccaag ttctcttacc ccaagtccta acttgatatc
     5761 tgttccccga ccagccatgt tggtagcaat cgtcacagca cctttttgac cagcattcat
     5821 gatgatctct gcttctttaa agtgattttt cgcattcagc acttcatgag gaatacgttc
     5881 ttgattcagc atatctgata ataactcaga tgtttcaacc gcaacagttc cgactaacac
     5941 aggttgacct ttgcggtagc gatctttgat atcttgcacg acagcgtgga acttgctttg
     6001 taatgttggg taaagcaaat ctggacgatc atcacggata accggacgat ttgtcggaat
     6061 ctggtatact tggatgttat aaatttctct gaattcttct tcttctgttt tagctgtacc
     6121 agtcattccc gcaagtttct tgtacatacg gaaatagttc tggaaagtga tcgtcgccat
     6181 ggttttcgtt tcgtcttcga tctctacgcc ttctttagct tcgattgctt ggtgaagtcc
     6241 atctgaataa cggcgtccat ccatgatccg accagtgaac tggtcaacaa tcagtacttt
     6301 tccgtcttgg acaacataat cgatatctaa aagcatgata tagttggctc tcaatgcttg
     6361 atccaagtga tgggtcaatg cagtattttc aatgtcgtat aagttatcca aaccgaatgt
     6421 ttcttctgct ttttcgatcc ctgcttcggt taaactgatt gtttttgatt gtacatcgat
     6481 tttataatct tcttcttctt tcaaacgttt cacgaagttg tctgctcttg tatataaagc
     6541 tgtggatttt tctgcttgcc ctgaaatgat cagcggcgtt ctcgcttcat caatcaagat
     6601 tgagtcaact tcatcgacaa tggcataatt cagcggacgt tgtaccattt gatggcggta
     6661 aacgaccata ttgtctctca agtagtcaaa tcctaattca ttattcgtac tataagtgat
     6721 atcacaatta taagcctcgc gtttttcatc cgaagatttt gagttgatat tcaaccccac
     6781 tgttaatcct aagaaattat aaagttcgcc catttccgtc gagtcacggg ttgctaaata
     6841 ttcattgacg gtaactacgt gtaccccttc gccagtcaga gcatttagat atactggcat
     6901 cgttgcggtc aaggttttac cttcaccagt acgcatttca gggatatttc catcgtgaag
     6961 cacgattcca cccatcaatt gtacgtggta tggatataaa cctaatacac gttttgctgc
     7021 ttctcggacg actgcaaaag cttccggaag taattgatct aatgtttctc ctttttggta
     7081 tctgcccttg aattcagcag tttttgcctg taattgttca tcagttaatg ctgccatttg
     7141 gtcagcgtat gcttcaactt tttttgcaat gctatctaaa cgttttagtt ctttcttatc
     7201 gttttcgata agtgaacgaa tcaaattggc catgcgttgt gtaatctcct tttccttctg
     7261 tgtattatcc agactatgta gactttactt ttcattttat cattagttat agataattga
     7321 aatatcttaa gcgtattaaa gtaagaactt cataatttat tcatatttca tctaaaagag
     7381 gaggaaactc
//
LOCUS       DANKKG010000125         7226 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000125 DANKKG010000000
VERSION     DANKKG010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7226
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.125"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(277..732)
                     /gene="tnpA"
                     /locus_tag="PW004_002307"
     CDS             complement(277..732)
                     /gene="tnpA"
                     /locus_tag="PW004_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HDL2727258.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            975..2401
                     /locus_tag="PW004_002308"
     CDS             join(975..1548,1548..2401)
                     /locus_tag="PW004_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
                     /protein_id="HDL2727259.1"
                     /translation="MAKYTFELKLKIVHDYLDGKGGSDYLAKKYSIKAPSQVKRWINA
                     YQEFGEEGLVRKRQKKKYSVQFKLDAIELYLTSELSYREVANTLNMNNPNLIASWMRQ
                     FREGGIDGLSKTKGCPPILSKKNEPKNKKKSTTKATSKERERIEELEKRVRSLQIENA
                     FLKELRKLRKQEAQQRRMKQSHESSQASEDRFKLVELLETLKFPKAIFMYWQKRLDRK
                     NPDQEIEAEMLKVREKHKDYGCLRMTNELRNRGFSINKKKVQRLIKKLGIKVTTYTRK
                     SRRYNSYRGQVGAVAKNRIHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLD
                     PFMDMFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYR
                     NKLKEYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYY
                     NNERIKQKLNWQSPVQFRKTTVTVV"
     gene            complement(2571..3533)
                     /locus_tag="PW004_002309"
     CDS             complement(2571..3533)
                     /locus_tag="PW004_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2727260.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(3535..4974)
                     /locus_tag="PW004_002310"
     CDS             complement(3535..4974)
                     /locus_tag="PW004_002310"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HDL2727261.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            5159..7105
                     /locus_tag="PW004_002311"
     CDS             5159..7105
                     /locus_tag="PW004_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HDL2727262.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         2320 a         1278 c         1243 g         2385 t
ORIGIN      
        1 attatttctg cgccagcatt ttctaatgtt tgaatttgtg ttttgtaatc ttggttgctt
       61 gtgctaattc ttgaatagcc gtaaattgcc aaaatttcat caccctttct ggacataagt
      121 ttctgacacc agttaaaatc aagtctatca gtaacccata cacccgtgag gacgggtgta
      181 tttttaatcc ccctagaagg ggtcgacact cgctgcccct taaaagggga tttttaaaag
      241 accgccaact gctcatcttc actcacttac ttctctttat tttttaaatg gttctacgta
      301 ctctctttta cttataatat atcgaatgcg atcatcagct tcttgttcac ggatgtattt
      361 ttcatttgtc ttttgattca atccgactgt actcacatag tatctcttcg accaaaagga
      421 tttgtttccg tatttatatt tgagatttgc gtgctgctcg tggattagta ccgcactacg
      481 acctttcaga taccccataa aactggatat acatattttt ggtggtactc gaacaagcat
      541 gtgaatatga tctagcattg catgtgcttc tatgatttcc acatctttca tttcgcacaa
      601 cttcctaagt atttttccaa gatcttttcg taattgccca tagattactt ttcgttgata
      661 ttttggtgta aaaataatat gatacttaca attccaacga gtatatgcta aacttttatc
      721 gtcatttgac atgaaaaaac tcctttcgat ctgatgagcg gttggtagcc actcactagt
      781 atatcgaaag gagtttttat cgctaaagat acttcttcca cgcgtagaaa cgcgcggttt
      841 tcttgtttcg cacgctttac gcacaaaact gactgaagtc attaagaaaa taaaagggtc
      901 taaaactttt aagttttgta ccgaccccca aaagttagac caaaaaatct aacttttgga
      961 ggtcggtatt ttttatggct aagtatactt ttgaacttaa attaaagatc gttcatgatt
     1021 acttggatgg taaaggcgga agtgattatc tggcaaagaa atattccatt aaggctccat
     1081 cacaagtgaa gcgctggatc aatgcctatc aagaatttgg tgaagagggt ctagtaagaa
     1141 agcgacaaaa gaaaaaatat tctgttcaat ttaagctgga tgcgatagag ttatatctaa
     1201 caagtgagct gtcctatcgt gaagttgcca acacacttaa catgaacaac cctaatttga
     1261 tcgctagttg gatgcgtcaa ttccgtgagg gtgggatcga tggactctca aaaacgaaag
     1321 gatgtccacc tatcttgtct aagaaaaatg aacccaaaaa taagaaaaaa tctacaacta
     1381 aagcaacgtc taaagaacgt gaacgcatcg aggagttgga gaaacgagtc cgttcccttc
     1441 aaattgaaaa tgccttttta aaagaattga ggaagctgcg aaaacaggaa gcccaacaac
     1501 gacgaatgaa gcaatcgcac gaatcatcgc aagcctccga ggaccgttta aattagttga
     1561 gcttctagaa actttaaagt tccctaaggc aatctttatg tactggcaaa aacgattgga
     1621 ccgtaaaaat ccagatcaag agattgaagc ggaaatgctt aaagttcggg agaagcacaa
     1681 ggattatggc tgtttacgga tgaccaatga actaagaaac cgcggtttct ctatcaataa
     1741 aaagaaagtt cagcgcctga tcaagaagct aggaataaaa gtaacgacat acacacgtaa
     1801 atcacggcgc tacaattctt atcgaggaca agtcggagca gtagccaaaa atcggatcca
     1861 tcgtcgtttc tacaccagta tctgccatca gaaaatcacg acggatacaa ctgaatttaa
     1921 ataccatgaa gtggatgcca aaggcattat ccgtcaaaag aaattgtatc tagatccttt
     1981 tatggatatg ttcaatagtg agattttatc ctatcgtatc tctgaaaagc ccaatgcatt
     2041 agcggtaatg gaaggtttgg aggaagctat ccagaccact aacgattgtc cctatcgtcg
     2101 tacgtttcac tcagatcaag gttgggcgta tcaaatgaaa gcttatagaa acaaattgaa
     2161 ggagtacaaa atctttcaaa gcatgtctcg gaaagggaac tgcttagata attcacttat
     2221 ggagaatttt tttggcttat taaaacaaga gatttttcac gggaaagttt ataactgttt
     2281 cgaggaatta aaatcagcca tcgatagcta tatctattac tacaataatg aacggataaa
     2341 acagaaatta aactggcaaa gtcccgtcca atttcgtaaa acaaccgtaa cagttgtgta
     2401 aaaatatcat tttagagcat gaaaaaagga gtggatttct ccactccaaa aaagtctaac
     2461 ttttgggggt cactacaagt tgacaactcc tttttgttta caaatcaatt ggatatctac
     2521 aaattgtatt ttctaacgaa ttttatagac attaaaaaat taatttaacg ttaaatactt
     2581 cctccagaaa gtattgagat tggtaaaata taatccttat ttgtcttttc gcctttaatt
     2641 tttttcagaa gaatatcaac aataagactt gcaatttcat caattggttg tcttattgtt
     2701 gtcaattgtg ggaaaaattt ctcaatgaaa gaggtaccat cgtatccaat aatttttaaa
     2761 tcttctggga tattaagttt aagttgatga gcaattttca tagtgagaat ggctgttaaa
     2821 tcatcagaaa caaaaatacc atctggtttg tgatttataa tagttgactt tatttccatt
     2881 tcacgacgga tagtagacag attattagga attttgaaaa tttttccatt tggattttga
     2941 aaagaaaaac ctagtgcacg taattctgtg ggagaatccg tgttatcatt tcctgtaatc
     3001 ataatggtat tttgacaacc atttttttgt aaagtcttag ctgcaagttt tcccccttca
     3061 aagttatctg aagaaacaat ggaaacatta ggagctaaat ttctatcaaa agcaacaata
     3121 ggtgctctca cacgctcata atcagcaatt ccaagattat ggcttgatga gattatgcca
     3181 tcaacttggt tggcttcaag catttcaatg tattctctct ctttagcagg atcattttga
     3241 ctattgcaaa taatcgtttt atatccatgt ttaaataact ctatttcgag atactcaatg
     3301 agttcagagt aaaagatatt actaatatta ggaaaaataa gaccaataag ctgaggtgac
     3361 tttccttgaa gtccacgtgc taaattattt ggtttataac caagagtttt catagcttca
     3421 tgaacttttt gttttgtttt ttctgataaa tatcccttat tattaatgac gcgtgaaacg
     3481 gttgttgggc taactccagc taattcagcg acatcagtta attttgctac cattttaata
     3541 ttttaattca aagtattttc cagtaggttc gcctgatttg atttgaatac cattttggtg
     3601 attttcaggg aatacacgac tagtaaacac tttttctcct ttattaataa aaatctcaat
     3661 gattgagtta tctacaaaaa tgttagcagt tgtttcttta gtatcaattg agcattcacg
     3721 aatcgtacca aagtcagtgg catattgaat gcctgctttg ctgcgatcta aaataatttt
     3781 tccttctttg gtatctacaa tcaagctaag tccatttcct ttactatcag agaaaagaag
     3841 taactccgat ttctgatttg gaggaaaagt tagttcaagc tcataagtat tatttgtttc
     3901 tgcttttgct gtaaattgtt cagaactagc acgtaatgat gtaatagctt ctactggata
     3961 ttggtaaagt ttaccatctt ttattgaaag ttcttttaca agactcattg ctccctgata
     4021 atcatactta tcagttggat aatcgatatc aggtagtcca atccaactga caatcagagc
     4081 acgaccatca ggagtattga atccttgagt cgcatacgct tcaaatccat aatcaagatt
     4141 ttgaatttct gaagcgccca caagttttac tttttctatg tcaagcgatt gacagatctt
     4201 aaaagtattc ggatagatat tcccataatt tagctcagat ttatccaacc cttgaggaca
     4261 ataaatgaga acaggttttt tgtctacaaa tactagattt ggacactcaa tcatgtattc
     4321 tgaaccagtg ccgccaaaat caaggttgcc aacttcctcc catgtttcca cgttattgtc
     4381 atccgctttg taaagtttga taaagcctga tttttctaaa ttttgtgctc caacaatcgc
     4441 atacaattgt ccttcataat taaacatttg tggatcacga aaatgatcag ttacatcagc
     4501 aggtttatga ataagaacat tctcaccttt cgtaatgttg tagtctttat ccatccatgc
     4561 tccgatttga agtgtatcac gttcccaatt ttcatttcga acgtttcctg tatagagtaa
     4621 gaatagattc ccctctattt catatgcaga accggaataa gcgccgtggc tatctcgttt
     4681 tgtgtctgga agaagttctg ctcctgttac atgaaaatga actaaatcat ctgattcatt
     4741 gtgtacccat tctttcaacc catgagcagc tccaaatggc caattttgat aaaaaagcgt
     4801 atattttccg ttaaaaaaag aaaaaccatt aggatcgttt aacaatcctg ttttaggttc
     4861 gatatgatag ctagcacgcc aaggtgatcg ttttacatta tccttaattt tatccatctc
     4921 atctttagtc caatcagaat aagcacgata tcgtatttct cgaggtaagt tcatatttta
     4981 ctccttcaat ttatcttttt acaactagta tggtaaacgt tttccgttga aaaatcaatc
     5041 atttcattta aaaaaataaa ctatatgaca aacgtttgac atatttttaa gatatgctaa
     5101 aataaatttg catttcagtg aaccgttttc acgtaaaaat aaaaaaggag tgattcgaat
     5161 gaattacagt aaagtagctt ccgacgtaat acaagcggtc ggaaaagata accttattgc
     5221 agctgcacac tgcgctacac gtcttcgtct cgtactaaaa gatgacacga aagtgaatca
     5281 aaaagcttta gacgaaaatc cagatgtgaa aagcacattt aaaattgatg gacaatatca
     5341 agtgattatc ggtgcaggtg atgttaattt cgtttatgac gaacttatca aaaaaactgg
     5401 actttcggaa atttcaacag atgatttaaa acaaattgct agtaaagata aaaaattcaa
     5461 ccccattatg gcattaatta aattattatc agatattttt gttccaatta tacctgccct
     5521 tgttgcaggt ggtttattaa tggcattaca aaacttttta acagctgctg gactttttgg
     5581 accaaaatca attgaagaaa tgtatcctgc gattgaaggt atttcctcaa tgatccaact
     5641 gatgtccgct gcaccattca tgttcttacc aattttagta ggtatttcag ctgctaaacg
     5701 ttttggagca aaccaatttc ttggtgctgc aattggtatg atcatgacaa caccagatct
     5761 tggtggctca actgaatatt ggaatatttt tggttatcat gtttcacaaa caaactatgc
     5821 ttatcaagtt attccagttt tagcagctgt ttggatatta tctatattag aaaaattctt
     5881 tcataaaaaa ttgccatcat ctattgactt tacattcacg ccattattat cagtaatgat
     5941 tactggattt ttgactttca cagttattgg ccctgtaatg ttattgttgt ctaatggaat
     6001 tacagacatt attgtttggc tatataacac tactggattt attggaatgg gtatttttgg
     6061 aggaacatat tcacttatag tcatgactgg tttacatcaa tcatttccag ctattgaaac
     6121 tcaattactt tcagcgtgga caaatggtat cggacatgga gactttatct ttgtagttgc
     6181 atctatggca aacgttgctc aaggtgctgc aacatttgcc atttggttct taactaaaaa
     6241 ttctaagaca aaaagtcttg cttcctctgc gggtgtctca gcacttcttg gaattacaga
     6301 gcctgccttg ttcggagtta acttaaaata ccgctttcca ttcttttgtg ctttaattgg
     6361 ttcaggaatt gctacagcag tagcaggatt acttaaagta gttgctgttt cacttggttc
     6421 agcaggtttc ttgggatttc tttcaattaa tgcaacatct attccatttt acgtattgtg
     6481 tgaattgatt agctttgtag ttgcttttgc aattacttac ttctacggta aaacaaaagc
     6541 tgctggtatc tttaaagcag aagaaattgc tgaacaaaca ccagttaatt cttcaaaagt
     6601 agcaacaaca gatccaatat ctactgatgt tgaatcaaca caaacaaaag tttcatcaga
     6661 aacaattgtt agtcccattt ctggtaaagt gattagtctt tcttcagtaa atgatccagt
     6721 cttttcatca gaagctatgg gaaaagggat tgccattaaa ccaaatggaa acacggtcca
     6781 ttctccagta gatggtacta ttcaactcac gtttgaaact ggacatgctt atggtattaa
     6841 atcaaataat ggtgctgaag ttctaattca tattggtatt gatactgtat caatgggagg
     6901 aaaaggattt acacaaaaag ttactactaa tcaaaaagtt agaaaaggcg atattcttgg
     6961 agcatttgac cgttcaatga ttgagaaaga aggtcttgat gatacaacaa tgattattgt
     7021 gaccaatact tcggattatt cagaagtaaa tatagtagct gaaaatacag tcactgaggg
     7081 tactgattta cttgaaatca aataaatata tttccctgag taaagatgga attttctttc
     7141 caaaatcaaa taatatagat taatcaaata tttgagaata acattttatt taatcttttc
     7201 atatatcaat tgtatgaaaa attatt
//
LOCUS       DANKKG010000126         7174 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000126 DANKKG010000000
VERSION     DANKKG010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7174
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.126"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(579..1595)
                     /gene="tsaD"
                     /locus_tag="PW004_002312"
     CDS             complement(579..1595)
                     /gene="tsaD"
                     /locus_tag="PW004_002312"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HDL2727263.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(1592..2056)
                     /gene="rimI"
                     /locus_tag="PW004_002313"
     CDS             complement(1592..2056)
                     /gene="rimI"
                     /locus_tag="PW004_002313"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDL2727264.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2063..2605)
                     /gene="rimI"
                     /locus_tag="PW004_002314"
     CDS             complement(2063..2605)
                     /gene="rimI"
                     /locus_tag="PW004_002314"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDL2727265.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(2589..3311)
                     /gene="tsaB"
                     /locus_tag="PW004_002315"
     CDS             complement(2589..3311)
                     /gene="tsaB"
                     /locus_tag="PW004_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HDL2727266.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(3502..4482)
                     /locus_tag="PW004_002316"
     CDS             complement(3502..4482)
                     /locus_tag="PW004_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2727267.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(4506..5990)
                     /gene="galT"
                     /locus_tag="PW004_002317"
     CDS             complement(4506..5990)
                     /gene="galT"
                     /locus_tag="PW004_002317"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HDL2727268.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6002..6991)
                     /gene="galE"
                     /locus_tag="PW004_002318"
     CDS             complement(6002..6991)
                     /gene="galE"
                     /locus_tag="PW004_002318"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HDL2727269.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT         1965 a         1560 c         1269 g         2380 t
ORIGIN      
        1 aatatcaggg tcactgctta ttttcctccc taaaacttct gaaagatccg gcagttttag
       61 atcactggac aagactcaaa gattttctat gtgtgtttca gttatgtaat attcactgtt
      121 tgcaaaaaat actgttagat taactagatg aagcgtcttg tcctcatccg atctagatta
      181 ttcgatattc ctcgacaata aacgattact cagtgagggc aatcagagga acggataatg
      241 ataaataagc tgtaaaaaat agcaaaataa caataatgat taaaagtatg taaaaaatag
      301 ggctttgaca aaaacgtgtc acagccctat ttcgattaaa cggtgtgtac ctgtcaactc
      361 ttcggtcaca agtcataatg ttcataaaca tgaggagctg ttttttgaaa ttgttccttg
      421 tgaatcagag ttaaacgaca ggcttacgaa ctcttgatta aacggcgttc aaaagcttgc
      481 atacgcggta ataagaacag ccaggtcaaa aacatgagaa gccgtttttc ctgggctgtt
      541 cttattactt gatgcagggc agctttttca caacctcttt attcagataa aaccaagctt
      601 ggatcagcat tcaactgata gctagcaaaa tttctttttt gcatttccac atgtgctgct
      661 gctccaatca ttgcagcatt atctccacaa agtcgaagag ggggaatcac tagttctacc
      721 tcagggagtt tagctgccaa agcatcctgc aatccttcac gcaacccttg attggccgct
      781 acacctccag ctataatcag ttgcttgact ggataatttt gacaagctcg aagtgtttta
      841 gtaatcaata catcgatcac acttgcttga aagctagctg ctaaatcatt tttgtctaga
      901 tcttctccac gctgttgtgc attatgcacc aaattgataa atgcactttt taatccgcta
      961 aaactgaaat cataattatc ttcatggatc atcgctcgcg gaaaatggta attatctttt
     1021 ccctgatgag ctagctgatc gatttctttt ccgcttgggt aagaaagacc taatactctt
     1081 ccaactttgt cataagcttc tcctgctgcg tcatctcttg tctccccaat gatttcatat
     1141 gatccatctt cttgcatata gactaattct gtatgtcctc cgctaacaag aagtgccatc
     1201 aatggaaact ggaatggttt aaccagacgg gctgcataga tatgccctgc catatggttt
     1261 acaggaataa gcggcaattg gtgtgcccaa gcaaaagcct tagctgcaga aataccaatc
     1321 agcaacgagc cgacaagtcc tggtccataa gtgacagcga ctgccgataa atcttctgcc
     1381 gacacaccag cttctaccag tgcatcttcc aagcataagg tgatttgttc tacgtgatgg
     1441 cggctagcta cttcaggtac tacgccacca aaacgtttat gactgtttat ttgagaagca
     1501 acgatgttag ataagatctc tgttccattt ctaacgaccg ctacactcgt ttcatcacaa
     1561 cttgattcaa tcgccagaat caattcttca ttcatttcat cgtctccgtt tttaactttc
     1621 tgctcataat gatcgcatct tccgtcggat tgcgataata tgatttccgt ttacctaata
     1681 tacgaaactt ttctgattca taaagttttc ttgctgcttc attagataga cgtacttcta
     1741 aaaaaacatg cagcgaatct tttatttgtt ccgtaatcag taattgtaac aaatgccgtg
     1801 catgcccttt tccttgttct gaaactgcaa cagcaatatt tgtgatctcg acttcatcta
     1861 ttactttcga gtatccaata aagccacaga tctggttatc ttcagctaaa agcagataat
     1921 cagagtgagg ctgttggatg tctgccaaaa actgcatttt cgtccaaggt gaaccatgtg
     1981 gataagcatt ttcgctaatc atccatacgc attctgccaa gtacgcttca tcaaaatctt
     2041 tttttctgca aatcaactaa tctcactcat tttctaaaag ttttatcata tccaacgcat
     2101 cttcttttgt ttcagtgtaa tatctttttt tgatcgttgt tgcttgaaac cccaatttcc
     2161 gataaagtct ttgtgcatct tgattactca tacgcacttc taatgacatc gtctcacagc
     2221 gattcattat agcaaagttc tcgatttcgt caataagcag cgaaccgatt cgtttacgct
     2281 gatatgccgg tcgaacggca atattcgtga tatggcaatc taaaccgaac gctcttgaac
     2341 cgataaaacc aatcattctt ccattatcaa agatacctag atatagatga ggaagcggtg
     2401 acttcaattc tgatataaag gcactttttg tccatggcaa ttcgccatta tagacttctc
     2461 gttcgacatc cagcagctct ttgatatctt ctgtagtcaa ttctcttagt acgtaacgac
     2521 gatcgatttg ttcgactgtt ttttctgtat aaatcatttt atcttgaaaa aagcttctgg
     2581 catacgctct aaattttttc aacataactt tcgtctccag gtgtgtgcgt ctccaaccat
     2641 ttttcttctg cttctactcg ttttaaatag cgaggaagaa aagcttgttt ctctgcaact
     2701 ggttcaacta aagcacctaa ttgagccaca gttacaccac ttggaatatc ccatgctgga
     2761 actgtattaa tcatcgcact aggcaatatt tcctttattt cagagaagaa tttgtgacaa
     2821 tcactcccga cgaaataaat agcttcattc agcttttgta gttgtgtgaa caaatcagaa
     2881 gcagaaatat gtacatcttc aagtacagtt tctaatctat tttctttcca gcggtatgcc
     2941 ccagcgtata cattttttct tcgagcatca aacatgggca caataatatt atccacaccg
     3001 atgcaattag ccgcgatggt tttcagacta gatactccta ctagctcttt atctaatgta
     3061 taagcaatcg tttttgctgt cgttacacct atacgaagcc ctgtataaga accgggacca
     3121 tcagaaaccg cgattcggtc aatgtctttt ggacttattt gaagatctgc aaacaattgg
     3181 tcgattgcag gcatcagtgt tacactgtgg ttgcgtttga tatttgtttg tatttgtcca
     3241 agaatttgtt cgtcttctac cacaccgatc gccaaagttt ggtttgcagt atctattcct
     3301 aatgtaatca tatatttgat cctttctgct atgacattct cgccttccat tgtagcatac
     3361 ttttttattc tgaggatgaa aataccaaag gcaaaactag tacaataata catgattatc
     3421 tgctggcggg caaattttgc cccttcctga aacacaaaaa agctgcggta tcactccatt
     3481 ttcccacagc ttttccatct tttatttcgt tgattctcga ataatcaact ctgtttccac
     3541 aataatcttt cttgaaaccg gtgctttgga attacttaat tgaagaatcg tctccactgc
     3601 tagctctccc atccattcgg tataaacttt tacagtagaa agcgagggcg ttacatattt
     3661 cgctacactg acatcattga agcccatcac agaaatatct tctggtacca ctaaaccttt
     3721 ttcttgaatc gctttcagtg cgccaatcgc taatgcatca ttagctgcaa aaagcgcatc
     3781 aggaacatgc ggattcgttt ttaaatattg cttcactgct tgatatcctg actcgacaga
     3841 aaaatctgct tcgataattg gaccttcgtc gtagcctttt tctgccaaaa cttgttgaaa
     3901 tgccataatt ctgggatctt ttagccgctg atggtttttc ttcgtatatt ctgttccagc
     3961 aagcatagcg atatgttgcg ctttatggga gacaaagtgt tcaatgactt tttgaacact
     4021 tgacttaaag tcgacaacta gagagtcgat tccttgagaa gtgccgtcag aatctaccaa
     4081 aagcaaggca ttttgggatc ctttcatttc taataactga gcttcgtcga attttcctag
     4141 tgcgatcgtt ccatctgccg gaagatcact aatggaatcc cacgtttctt tcaataaagt
     4201 aacgtttgct tcttctgcct ttttttcgat tcctaggcga atagataaat aataaagatc
     4261 ttcaagctct tccgtatcgt cgtaccactg gatcaggcga agcgtttgct tagtatttgc
     4321 tgcattttta cgatgtttcg tataatttaa gcgttctgct gcttcaaaaa ttttttgctt
     4381 tgtggctaag ccaacggaaa gatcaggatc atagttgaga actcttgaaa cagtagcagg
     4441 agaaactcct gccaactgcg caatgtcttt aattgttgcc atttgtctct cctccttttt
     4501 tacgtttata aaatcgcagt aaatcgttca aacgctgcct gtcctttttc atctcgcttg
     4561 aatactccag cgtcttccag tacacgtgca aaaacctgac ctacagattt ttgtacgaca
     4621 tcggtgaccg tatctttcgt caaatcagga tgttcttttt ttaattgttc tgcccacgga
     4681 cgatgatatt cagccatctg gttttcttct cctaataaga aatgctctac ttctactagt
     4741 tctggtttca aacgaggtgg taaaacagca agccccatta cttcgatcaa cccaatgttc
     4801 tcctttttga tatgttggac atctttatgt ggatgaaaaa tcccatcagg atgttcttct
     4861 gaaacattgt tgtctcgaag gacaaggtcc aactcaaagt aattgtccct tcttcgtgca
     4921 attggcgtaa tcgtatggtg tggtgtacca tctgctgaaa aagctctgat agatactgtt
     4981 tcatccgaat atgctttcca tttttccaat actgagttag ctgcttcaat aagcaagttt
     5041 ttctctgttg attgcaaacg gatcacagac atcggccatt tcacgactcc tgcgatgact
     5101 tgcggataat cttctagttt gataagtttt tctatcggtg cttttgccat aggaaattca
     5161 tgacgtcctg cctgataatg atcatgagaa agaatcgatc ctccaacaat tggtaagtca
     5221 gcattcgaac caacaaagta atgaggcaag acttcagtaa tccgaagcag tctagcaaat
     5281 gtttcctttg tgattttcat cgggcggtgt tgttctgata aaatgatcga atgttcattg
     5341 taataagcat aaggggaata ttggaatccc cagctttcac cgtctagatt cattcgaata
     5401 atccggtgat tggtgcgggc tggatagttc aagcgacctc tatatccttc gttttccatg
     5461 caaagcatgc aggtaggata gtctactttt accgcctcgc gttctgctgc gatttgtttt
     5521 ggatcttttt ctggcttaga taaattgatt gtgatttcca gttcaccata ctccgactct
     5581 gccggaaatt tgatgttttt tgcgatggct cttgttttga tgtagtcatt tttcttactt
     5641 aaattaaaga aatactcagt tgcttcctct gggcttttcg aatagtgctg tgcaaagaat
     5701 gcattgacga ctgaaggtgg cggtgtgaga aaatccatta attgcgcttc aaaaatctct
     5761 tgttctgctg gcaattctga aagaacacca ttttctcttg ccttagcgac aagctgatcc
     5821 aataagtcta cagattcttc cttaactggt cttacttcaa tgctttctag agcatcttct
     5881 cctaccatcc ctaaaagacg attctgtaaa tataagcggt ccatttccat ccatccgcca
     5941 gcttcgatcg caagtgtggc aaaatcacaa atcgtttgac tgattgacat atctcattcc
     6001 cctattcttc ataaccattt ggatggctga catgccaatc ccatgctgtt ttgataatcg
     6061 ctttgatatc tgtgtattca ggtttccagc ccagaatctc tctcgctttt tcacttgaag
     6121 cgactaatcg gctaggatct ccagctcttc ttggtgctat ttctgctgga atttcttttc
     6181 ctgtcacttc tctagctgct tctagcattt ccttgactga gtatcctttg ctgctgccta
     6241 aattgaagaa gttgctttca tttccttctt tcaagtattc caaagctaaa atatgtgcag
     6301 caatcaaatc ttctacttga acataatctc tgatacatgt tccatctggt gtatcataat
     6361 cgtctccata aacagccaag gcttttcttt gaccaagagc tacttgtaaa atgattggga
     6421 caagatgcgt ttctggtgta tgatcttctc cgatagatgc atctgctttt gctccggcta
     6481 cattgaagta gcgcaaagcg acatagcgca tgccatatgc ttggtcacac cacttcatca
     6541 ttttttccat catcaattta ctttcaccat acggattttt aggattagct ggtgtatctt
     6601 ctgtgattgg agactctttc ggctctccat aagttgctgc agtagaagag aatacgattt
     6661 tattcacgtt gaactcgtgc atgacttcaa gtaagatttg cataccgtat acgttgttgt
     6721 tgaaatacat caatggtttt tcgacagatt ctcctactaa agagcttgca gcaaaatgga
     6781 ttactccttc aatcggttcc ttttcaaata cggaacgtag aaattcttta tcccggatgt
     6841 ctccttcata aaaatgagca tctggatgta ctgcttgttt gtgacctgtg agcaaattat
     6901 ctaccacagc tacttgatac cccttttgaa ctagctggtc tacagcgtgc gaaccgatat
     6961 aaccggctcc gcctagtact aaaattgtca taaaaaacac tcctttgatt agtaaaattg
     7021 tgtttgttga ttctatataa tttaaaaaac gaagtttact tttatggtga aaacaaacaa
     7081 agtaaaattt cgtaaattca tatggtaaat ttataataaa acatttagta aacattttca
     7141 agggtttttt gcaaaggttt acaaacaaac gtaa
//
LOCUS       DANKKG010000127         7067 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000127 DANKKG010000000
VERSION     DANKKG010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.127"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..325
                     /locus_tag="PW004_002319"
     CDS             <1..325
                     /locus_tag="PW004_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HDL2727270.1"
                     /translation="KGEARHALCRNIFYGRKGKLYQTYYDGMEEQLNALGLVTNAIIY
                     WNSLYLEKVFEQIKTEGFDCSEEVLRKQSPLISDHINFVGKYSFRYEKELSNGILRPL
                     NIENY"
     gene            490..717
                     /locus_tag="PW004_002320"
                     /pseudo
     CDS             490..717
                     /locus_tag="PW004_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            843..1814
                     /locus_tag="PW004_002321"
     CDS             843..1814
                     /locus_tag="PW004_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2727271.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            1953..3266
                     /locus_tag="PW004_002322"
     CDS             1953..3266
                     /locus_tag="PW004_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDL2727272.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDSGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFVWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMLLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQISGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            3309..4175
                     /locus_tag="PW004_002323"
     CDS             3309..4175
                     /locus_tag="PW004_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2727273.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQLGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            4172..5020
                     /locus_tag="PW004_002324"
     CDS             4172..5020
                     /locus_tag="PW004_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2727274.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            5034..6506
                     /locus_tag="PW004_002325"
     CDS             5034..6506
                     /locus_tag="PW004_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HDL2727275.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            6523..>7067
                     /locus_tag="PW004_002326"
     CDS             6523..>7067
                     /locus_tag="PW004_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL2727276.1"
                     /translation="MIYGSIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMA"
BASE COUNT         2189 a         1214 c         1531 g         2133 t
ORIGIN      
        1 taaaggtgaa gctcgtcatg ctctatgtcg aaatatattt tatggtagaa aaggcaaact
       61 ttatcaaact tattacgacg gtatggagga acagctcaac gcattaggat tagtaactaa
      121 tgctatcata tattggaatt cactatattt agaaaaagtc tttgaacaaa ttaaaacgga
      181 agggttcgac tgttccgaag aagtgttacg caagcaatct ccattaatta gtgatcacat
      241 taattttgtt ggcaaatata gtttccgcta tgaaaaagaa ttatcgaatg gaatactacg
      301 tccgttaaat atagagaatt attgagaatg ttcgtctttt ggttcctacc gtgcattttc
      361 gcacggatgt tccagatact atttttgaat gtataaaatt gaaaacacaa gaatactaat
      421 aaatcaacga ttcttgtgtt tttaattttt cactaccgtg cattttcgtt tctatgttcc
      481 atgtacccca atcttgaaaa attaattaaa gacttagata aagccattga cgacttagtc
      541 gtggtgctac cggagtatca atgtttaacc agtatcccag gtattggtcc tgtttaggct
      601 gctggaatac tcgctgaaat tggtccaatt gaacgttttt ctgatgaaat aaaattgaca
      661 aagtatgccg gcctatattg gcgacagagt caatccggta aatctgaata cgaaaatttt
      721 tttacgaaaa gcttcacgtc taatatctat tttaaaataa aaatattcct gctaatttgg
      781 aatcgtttcc ttcttttggt ataataatac ctgaaattct tttgagagga gttgttcgtg
      841 aagtgaaaac tactatgaag gatgttgctg cgttagcagg agttggtgtg ggtacggtat
      901 ctagagtaat aaatggagta aaggtgaaag aggttaccaa agaaaaagta atggctgcaa
      961 ttagtgagct taattatgag cctgatgaat atgccagagg tatgaaaacg aatagtagca
     1021 atacaatagc tcttatttta cctacgattt ggcatccctt cttttccgag tttgcctact
     1081 atgtagagga agctctcagc caaaaaaatt ataagctttt tttgtgtaat gcgaacggca
     1141 gtgcggaaaa agaaaaagaa tatatccaaa tggtcaagca gagtaaagta gacggaatta
     1201 taggtattac ctattcagac attgataaat atgtatcatc aaatttacca tttgtcagta
     1261 tagaccgcca tttttcggaa caggtttctt atgtaacggc agataattat ttaggtgggc
     1321 aaattgccgc tcaagaacta cttactcgta actgtaaaaa cttgtgctat gttgggggtg
     1381 tttcacctta ccctaacgaa acaaacaatc gtaaagctgg tttttacaat atttgtgagg
     1441 aaaataaaat agctgtgaag gtattggata tgcctgaacc aattagcaat ttagaggaac
     1501 aattgctcgc ttttttttcg gataatcctg aaattgatag catttttacc atcaatgatt
     1561 tcatggcttt ggatgtgata aaagcccaaa aaaaactagg gaaggaagca cctaaggatt
     1621 ttcaaattat tggttttgat ggagtcaagc tatcgttgca aaaagactat ttactatcaa
     1681 cgattgtaca gccgatagag gaaatggcca aggtttcagt agaaacactc atggcgatga
     1741 ttttaaatga agaaccgcct aagcgtgtgg tagtaccggt tttctttcac gctggagaga
     1801 cgactaaaga gtaaaaaacc ggtttataac gatttttttg agataggttg acagaaagtg
     1861 atttcaatga tatcttaaaa ccaattaatt ggaaacgtta ccaattaatt gaaaaataaa
     1921 acggaaacgt ttcaaaaata ggaggaacat cgatgaaaaa gagaatgtta gcaattgcag
     1981 caattttagc attaacagct ggaggacttg caggatgtgg cagtaaggag aaaagttcag
     2041 gtagcagtga ttcaggagaa attaagattt gggttcagtt ttctgatgaa acgcctgaag
     2101 gaaaagcttg ggcagagatt gtgaacaatt ttaatgatga atacgagggt gaatataaag
     2161 ctgttactga gtatattcct cgtagcggaa gcggcggtgg atatgaggat aaagtaaatg
     2221 ctgcaattac aaccaatacc cttcctgatg ttattacgtt agatggaccg aatactgcag
     2281 cttatgctca ttcaggagtc attgctccat tagatgatta tttgaaagat gcggacatgg
     2341 atgatgtatt agacagcatt attcagcaag gaacctacga tggcaaaatg tatgcgtttg
     2401 gtttctcaga gtccagtgtg ggtgtctatt acaataagtc gttgttcaaa gatgctggaa
     2461 ttacagaaga tcaattgccg acgttagaag atccttggac ttggacggaa tttcaagcaa
     2521 tttgccaaaa gctgactgat aaatacaaaa cgcctgcaat tgatatgcaa atggcgtcgg
     2581 gagacgaaat gctgacctac gcatatacac catttgtatg gtcgaatggc ggcgatgtcg
     2641 ttaatgctga tggaaccaaa gccgaagact attttaacgg taaaaaatcc gttgaagcgt
     2701 tggaatttat tcaagaattg gttgaaaaag gttacaccac aatcagtcct attgaaaaag
     2761 gatttgaaac tggcaaatat gccatgctgc tgagcggttc ttggacaatg gcagatttag
     2821 aggaaaacta tcaagagatt gattatggta ttttgcctta ccccgtttca gatgcaactg
     2881 gggagttagt ttcaccaacc ggaagctggc agttagcaat gactgagaaa acagagaaaa
     2941 aggatgcttc tgcggccttt attaagtttg caaccagtac agaatctagt cgtattatga
     3001 gtttagggaa cagtgttcta cctatcagaa attcaacagt tgatttaatt aaagatgaag
     3061 tgtcggaagg aatgcaggta ttaattaagc aaaaccaaat ttctggacat gcccgcccag
     3121 tggttgtagc ttatccgcaa gtatctagag cattccaaca agcagttcaa gatattagct
     3181 acttcgagga aaacccagat gtacaaaaag tagcagatag tcgcgctgct gaaatgcaac
     3241 aggcaattga tacttcacta aaatagaagt ttctaaaggg ggccaatcaa gtgttaaaaa
     3301 aaccgacaat gaaagacaat ttgataggtt acggctttct agcgccagct ttgctgctgc
     3361 tgacgatttt tctgatcata cccgtaatca tggtaattta ttatgccttt actgattatt
     3421 atttgttaac gccagatgct agacaattta tcggcttaga aaattttaag cgactgttcg
     3481 ctgatccaat ttttgtaaaa agtatttgga acacggctaa atttgttgtt tggattattc
     3541 cggtccaatt aggggcagct ttaggaatgg cacttattat caacaaaaat cgtaaaggaa
     3601 atatcttttt taaagtagcc ttttttgcgc cagtcgtgat gtccttagtc gttatttcga
     3661 ttttatggtt gtacttgtta aatcctaatg aaggtctggt taatacgcta tttgcaaaga
     3721 ttggaattgc ttcgcagcct tttttgacta gtcccaaaca agcaatgtat acgcttgttt
     3781 tcgtatcggc atggcaaggt gcaggatatc agatgctgct gtttttaggg ggaatgcaaa
     3841 atattccgca agacgtttat gaggcagccg aaattgatgg tttttctaag tttcagcaat
     3901 ttcgctatat caccatgcca ttattaaagc ccacggcgat ttttatttta cttaccacat
     3961 tgatttcggc ctttaaacta atcattcagc caatggtaat gactcaaggt ggaccaatga
     4021 attctactat gacaatggtt tattacattt accaaactgg atttacagac cgtatggtag
     4081 gctactccag ctccattgcg ttaatcttta ccactatgat tggtttaatt acaattgctc
     4141 aaagaaaatt ggtaaaggag gatgatccag catgaccaag aaatttggac cgctaatgat
     4201 cgtagaatat tgtttgctgt tccttttagc ggcattattc attttcccaa tgctgtggat
     4261 ggtagtttcc tctatgaagc ctgaagcaga tgtctatcaa aatttagctt ccttcaaagc
     4321 ttttttacct agtttgaatc cagctaattg gtttaaaact tatcaagaag ttatttcccg
     4381 gttcagtgtg ggaacttatt tattaaacag tattttttat ggtttaacct ttgcctgtgg
     4441 gtcaatcatt gtaaactctt tggccggttt tgcctttgcg aaggttaatt tttcaggcaa
     4501 gaaaattatc tttggagctt tgttagctct gctgattgtc ccgatggaga cggttttaat
     4561 tccgcaattt accatcgtta attcactagg cttagtgaat actcgcttgg cagtgatttt
     4621 accagcgatg gccagcgcgt ttaatatcta tttgtttaga aatttcttta tcgcgattcc
     4681 agaagaaatc attgaatcag cgaaattaga tggtgcaaat attgttaaaa ttttctttca
     4741 catcatgctg ccaatgtcta aaccagccat agccacagta ggtgtcttat cctttatctc
     4801 cagctggaat gattatattt ggccgttaat ggtcttaact gacagcagca aattttcaat
     4861 gcaagtggca attactacga tcaatactac acagccagtt tatatcaatc aagtgatggc
     4921 tgttttaacc attgcaacga ttccactgat tattgtttat atcgttgcac aaaaatatat
     4981 tctgcaggga cttggcggtt ctggaacggg tataaaatag gagtgaatag tgaatgttgc
     5041 aaaaactttt aaaggccaat gagtttattg agaaaaatgc tcaaacagtc aatcaagaat
     5101 accggaataa atttcatttg atggctccga ttggctggat gaatgatcca aatggttttg
     5161 tttattatca agaggagtat cacttgtttt atcaatatta tccttacgac agcatctggg
     5221 gaccaatgca ttggggccat gctaaaagca aggatctaat tcactgggag catttgccag
     5281 tagcactggc accaggtgaa agctatgaca aggaaggctg tttttccggc agtgccatag
     5341 aaaaggatgg caagctgtat ttgatttata caggacacaa tgttgtggaa gggcaagttc
     5401 gacaagttca gtgtttggca gtttcagaag atggcattca ctttgaaaaa tatgccggaa
     5461 atccaatcat cgcagaagat cacttagctt ctgttgcaac gaccgaagat ttccgcgatc
     5521 ccaaagtgtt tcagcgaaaa gaagactatt ttactgtagt cgctactaaa actgcagatg
     5581 ctcgtggaca aattgtgatg ttccattcca aagatttact aacttgggag ttttattccg
     5641 tattgctgga gggaactcca gaacaaggca ttgtatggga atgcccagac ttgtttgaac
     5701 tagatggcaa agaagtcttg attctttctc ctattcaaat gactccacaa ggcaatgctt
     5761 accaaaatac gaactcaact gttgctttca tcggtactgt cgattggcaa acaggcaaat
     5821 ttctagtaga gaattttcat gaaatcgatg gcggtttaga tttttatgca ccccagacct
     5881 gtgaaaacga agcaggccaa agaataatga ttgcttggat gcaaatgtgg cagcgtaaaa
     5941 tacctacgaa tgatctgggc catggttggt caggttccat gaccttgcct agagagctga
     6001 gggtggctga taatcaattg ttgcaacggc cagtttcatc aatttatcaa gtaattgatc
     6061 cgcaggaaag gattgaatgg gcgaagactg aagagaatcg cttcttactg aaggacttag
     6121 tatatgatca gcactattta cgcttggaaa tcaatttaaa acaggcaacg aaactagaac
     6181 ttcactatgc gaagggaccc gaccatacat tactgcttaa ctatgatgtt gaacgggagt
     6241 gctttactgc ttccagggaa cacatgccgt atgatttatc aggagctgaa aaggaagtgt
     6301 tgaaaaagcg cagtgtgacg attcctttgg aaaataattg tctaaccatc gaaattttcc
     6361 gtgatacttc agctattgag ctttttctaa atgatcgtct gactatgagt acaacttttt
     6421 atgagacaga aaaaggacag gattttcttt tgctggcaga cagtgaagta accttttcat
     6481 tggaaagcgg cttaattaat ctatagaagg gagagctcag tgatgattta tggttcaatc
     6541 gaagcaggcg gcacaaaatt tgtgtgtgcc attggcgatg aagaaatgac gatcaaggaa
     6601 agagtgagct ttccgacaac gacacctgag gaaacaatac ctttagtcat tgacttcttc
     6661 aaacaatacc aagcagattt agcagggatt gggattggct catttgggcc tatcgatatc
     6721 catcgcgatt ccgccactta tgggtacatt acctcgaccc cgaaattggc atggcaaaat
     6781 tttgatttca tcggtaccat gaaaaaagaa tttccgatcc cgatttcttg gacaacagat
     6841 gtcaatgcgg ctgcctatgg tgagtatgtc ttcggcagtg gcaaaggatt gagcagtgtc
     6901 gtttactata cgatcggtac gggtgttggc ggcggtgcct tacaagaagg gcgctttatc
     6961 gaagggttta gtcatccaga aatggggcat atgctcgtcg tccctcatcc aaaagatgac
     7021 tttgctggca gctgtccatt tcatggcaac tgtttagaag ggatggc
//
LOCUS       DANKKG010000128         7029 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000128 DANKKG010000000
VERSION     DANKKG010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7029
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.128"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..491
                     /locus_tag="PW004_002327"
     CDS             <1..491
                     /locus_tag="PW004_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2727277.1"
                     /translation="RFRTSHSENQKKYRRLKRYWKLLLKDSTTLEPLKRHYHRLFKRP
                     ISQTEIVDELLSYNEELRTAYHFCQLLRYYFVKRDTEGFQETLKTISSTLPQSFKKKF
                     TIFHRYQSGISNAFKVPHSNGVTEGLNNKIKLIKRIAFGYRNFYNFRARIYLQQGLIF
                     EN"
     gene            complement(742..1320)
                     /gene="pgsA"
                     /locus_tag="PW004_002328"
     CDS             complement(742..1320)
                     /gene="pgsA"
                     /locus_tag="PW004_002328"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HDL2727278.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(1529..2452)
                     /locus_tag="PW004_002329"
     CDS             complement(1529..2452)
                     /locus_tag="PW004_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2727279.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(3190..4476)
                     /locus_tag="PW004_002330"
     CDS             complement(3190..4476)
                     /locus_tag="PW004_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDL2727280.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(4469..5737)
                     /locus_tag="PW004_002331"
     CDS             complement(4469..5737)
                     /locus_tag="PW004_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDL2727281.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            5989..6450
                     /gene="mscL"
                     /locus_tag="PW004_002332"
     CDS             5989..6450
                     /gene="mscL"
                     /locus_tag="PW004_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HDL2727282.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(6509..6955)
                     /locus_tag="PW004_002333"
     CDS             complement(6509..6955)
                     /locus_tag="PW004_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2727283.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
BASE COUNT         2004 a         1499 c         1247 g         2279 t
ORIGIN      
        1 atcgttttcg aacctcacac tctgaaaatc agaagaagta ccgtcggctc aagcgatatt
       61 ggaaactctt actcaaagat tccacgacat tggagccact caaacggcat tatcatcgac
      121 tgtttaaacg accaattagt caaacagaaa tcgttgatga gctgttaagc tacaacgaag
      181 aattgcgaac tgcgtatcat ttttgtcaat tattacgcta ttatttcgtt aaacgagata
      241 cggaaggctt tcaagaaacc cttaaaacaa tttcatctac gttacctcag tcgtttaaaa
      301 agaaattcac gatcttccat cgctaccaat cgggtatttc aaatgccttt aaggtgcccc
      361 actcaaacgg cgtcactgag gggctgaata acaagatcaa gctgattaaa cgaattgcgt
      421 ttggttatcg caacttttat aactttcggg cacggatcta tttacaacaa ggtctgatct
      481 ttgaaaacta aaaaaatatg cggcgcacag aacgcgccgc atatcggact aagttttatt
      541 caaatttgtc ttcgccaaca caatttgacg aagagcccaa aaatcttgtc acagctcctt
      601 gttccaaata aacagtgttc tcctgccaaa acctcggtca taagtcacaa tatacataaa
      661 acatgaaaag ctgttttttg aaattatccc ttgtgactca gagttagacg gcaggctcac
      721 aacctctcca ctaaacgata cttacattga tcctttgaac acaccgctgt ttttgatgaa
      781 gtaatccaca cctgaataga tagtaaagat cagacaagca taaagcatga tttgatctac
      841 tggaataccg atcaggctga atgggatatt attgataaac agcaagataa tagcaaccat
      901 ttgtgtagcc gtcttgactt ttcccggcca agctgcagcc atgacttctc catgttcgac
      961 aagtagtaaa cgcaagcctg tgacggctaa ttcacgacag acaatgatcg ctactaccca
     1021 accaggggcc tttccttggt caaccaacat gataaaagca gtcataacta gcattttatc
     1081 tgccaacggg tccgcaaact tgccaaaatt agtgacaagg cctcgcgcac gggcaatttt
     1141 tccatctaac cagtctgtga tactagctaa agcaaaaata actgcaccta ctagctgtgt
     1201 gacagccagt gtggtgtctc ctacgttaaa ttgtccccaa tctaaaggta aacctacaac
     1261 caaaatgaaa atcgggatca tacaaattct aagtacagtt aatttatttg gtaaattcac
     1321 actatcgact cctttcttcc ctattctacg tgactatcag cgaagtttcc accgatatta
     1381 tttacttttc caaatgaatt tattcaaagg agtattttta ttgaaattga tctttttgaa
     1441 ttaacgaaga aggaccgcga cacagatttg tcacgatcct tcttttttat acctacaagt
     1501 aaacagatgc gtaaactaat ttatacgatc attctgcgta ttgaagagta aggttcacat
     1561 tttttggact ctgattgttt tcccctggat taactggtac ggattctcca ttcgctttga
     1621 tcgttgcatt gcttgctgca ccaatcgtga tggtcgcatt tgttgctgtt tccggtaaca
     1681 cggtactctg tgtttcattt gctgccagtg tcccttgata aacataggca ttgtctacta
     1741 gcacacctaa ccatacacga ttgacagcag taaattcaag ggttacaggc ttggtagcgt
     1801 ctgtgatatt tactgcgatg gcactgccag aatcttctcc tgtagctatt gccattttct
     1861 tttcttcact tgattttgtt gttgattcta ctgtagaaga ctctttggtc tgcgtgcttt
     1921 gctctgagga agaggactcc tcgactgatc cttctacttt aagcgaagaa gctgtgtctc
     1981 cgattattgg atcagcttga cgatcttgcc aagtcatata gcccacgaca acgacaatcg
     2041 ccaaagcaac caatcctaat aaaatgacag gaagacttgt ccaaaagcga cttggatgcg
     2101 attcttcctg atgcattgct tttctagaag tacttactgt ttcaggctgc ggacgttcag
     2161 ggaccgatcc tgtcaatttg cttttcccat caaaaacatc gactaaatgg tctccatctt
     2221 ctcctacagc ttcagcgtat tgccgaataa atgcccgcac gtaaaattta ccaggcaatt
     2281 ggtcaaaatc atcattgtcg attgcttcca aataacgttt ttgaattttt gtaatctgct
     2341 gtaattcatc taaagatatt tttttattca ggcgtgcttg ccgtaatttt tctccgatgg
     2401 tttcaaaagc cactcgttca tcttctccag tctgtttgat tctccgaagc atctgctgtt
     2461 gcaacgaaag acgttatgtt tgggtgctgt gacaaaagtc atgtcacagc tccttttttc
     2521 cgaataaaca ttgttctcct gccaactcct cggtcacaag tcacaatatc caataaatat
     2581 aagaagctgt tttttgaact tgttccttgt gactcggagt taaacggcag gctcacgacc
     2641 tctttcttaa acggtgttca atcagctgtt cctcggtatt agctcaaaaa ccggcaaaat
     2701 ataaggagat atttttccgt atgtttctct aatactcagg tcaaaacgct gatttcacaa
     2761 cctcttcctt aaacggtgtt caaaagcttg catctgcggt aataagtcca accaagttaa
     2821 aaacattaag aagctgtttt tacttggctg ttcttattac ttgatgcagg gcagcttttt
     2881 cacaacctct tggtacacgg tgttccagaa gtagcttctt cggcaataag ccgaaatatc
     2941 acaaaaattt gagaagcaat tttcgtaaat tccttcttat ttcttgaagc tgaccacttc
     3001 tgtcacaacc tctttgatta cttcgtttga ttttatttta atataagttg attaaaagag
     3061 agcatgattt gttttatttt ctcactaatt tttccaacac aactaagaat accataaaag
     3121 ctgattttgg aggaggttcc tactacaagt ctaaaacgaa atttaagact tttcctcata
     3181 aatactggtt tattttggtc tcatgtaaaa ctcgctgaag gcctcttcac ttataaatgc
     3241 ttccccagct gccagtacgt ctttcatttg gatcgaatcg atgatttcag gaagatcaaa
     3301 taaagttcga tcgccaaata aactttgcgt aaattgattg gcaatatatt cgatcgaatt
     3361 taatgattgg aaatattgtc caagcatttt cttcttcagc aaatcgagat ttgtttctga
     3421 cacttccgga tcatcagcga atccaagaat gatttctttg actttttctg ctgctttctc
     3481 tggttcgtcc gtatctccgc tgaagtccgc aaagtgaaat tcacgatcca agctgaattc
     3541 aaaaccaaag ctgtcatcga taattccttg attgtacatc gctagatagt ttcgagaagt
     3601 attgcctaaa atcatttgga acaacagatt gattgctgtt ttatagcgta acagttctgt
     3661 tccttcttgc ggcaatgtgt ctaatccttt aataccaagt acaaatttat ctcgtgtaat
     3721 ggctgcttcc aatgcacttt gtttgatgat ttcttttgtg ttttcaggga aataacgtac
     3781 aatttcttgc tttggaggga aattttttgc ttcttggttt tcccgaataa gtttcatcaa
     3841 tttctcaggc tccatcttac caactacaaa taaaaccata ttgcttggct gataaaacgt
     3901 ccgatagcac gtatacaagt cttgagctgt gattttgtcg atactttcaa ccgtgcccgc
     3961 aatatcgata tgcaatgggt gtgtaggata caagttattt agtatgccaa aaaacatccg
     4021 ccagttaggg tcgtcttcat acatttggat ttcctgaccg atgattcctt tttccttgtt
     4081 tacagtttct tctgtgaagt aaggtgcttg tacaaaatcg atcagagtcg tcagattctt
     4141 ttctacctga tccgtcgtag aaaacagata gctggttttc gtaaaacttg tgaaagcatt
     4201 ggcggaagct ccttgtttcc caaataattg aaagacatca ccatcttctt tttcaaacaa
     4261 cttatgttct aaaaaatggg caattccatc tggaactttc accttttctt tttcaccata
     4321 gggaataaat tcgttatcga tcgaaccata attcgtactg aacaaaccat atgtcttgtg
     4381 atagtcattt ttaggcaaca aatacacggt caaaccgttt ggcagtactt catgataaag
     4441 tgtttcattg atttgctcat attctgtttt attcatttgc atttcctcct gctaaaaaga
     4501 agattgcctg caattcgatt tgctcagcta cttgttgtat ctctttgatc gttacgctct
     4561 ccatacgttt caaccattct tcatcagata attttgtttc cggcagccag ctgtccaaaa
     4621 aagaagcttc gattgccgct tgcggagagt ctagagaaag cagatattgg tttctcagca
     4681 ttgcttttgt ctgagacagt tccaactctg ttatttcacc atttcgcaaa ctttctaatt
     4741 gctcgtgaat cagatgaagt acttgatttc ggttcttttc atcgataccg gtttggacac
     4801 tcatgaatcc tcggaacgta tccacgctac tagaggcata ataagctaga ctttctttct
     4861 ctctgacatt catgaaaagt ttagagtgag gaaaaccgcc aaataaaccg ttaaagacca
     4921 ttagcgcaaa acgttctggt tcatcgtaat acacatttgt ctgataagct aggttcagct
     4981 ttgcttgaac gattggctca cgtacttgtc gttccttcac gatattcact tgaggttggg
     5041 tataaaacag atctggataa actgtttccc gcgactcaaa tggtaattgc ccaactgctt
     5101 cagctgcttt gtccggatca acatcgccga caacaaaaat gtcgatttga tcatttgcca
     5161 tcatttcttg atagtaggac gctagcgaac tagcagtgat cttgtccaaa tcttcgacta
     5221 cgcccaaccc tgggatcttt tgttccgggc tgttttcaaa ataaagttct tgcaaggcat
     5281 aggaagcaaa agtttgttta tcttccttca aactttccaa atatagacgc agattgttct
     5341 tttccaagtc aaacgtcaac tgatcaaact gatgattttt aatatttgga taaaataaaa
     5401 cctcttttaa aaaatctacc gcttgagaaa aaaggtttgg atcattgaca tattcaccat
     5461 tcacaaaact gatccctgcg tttagccaat gtaaatttcc tttttttcta actgataatc
     5521 caaagcttgc gccataaagc tctgcaagtt gcgcacttaa ctttgtctga tccggataat
     5581 tcaaactatt tgtttccaac atgctgctga gaagtgaacg tttcgtgatg acttcttgtt
     5641 gcagacgcgc agtaaaacgg ataaaaatcc ggatcgtttt gtattgtttt gttggtaaga
     5701 catgaagatt cacgccttct gccaattgat acaccatatt tatatgctcc ttttcacagt
     5761 tgattgcccg cttactcttt taatgggtca acctgccttt tttacttatt cctctgcttg
     5821 aaatttataa gttcaggcaa ttcgccgttt tttctaaatg atgttacact ttatagtata
     5881 gcatagatgt tttttatcaa aagaaacaga cggctaaaag cgtatattgt ttttttattt
     5941 tttatttttc gatatactaa agacaaataa aagaagggag ggtcattcat gatcaaagaa
     6001 ttcaaagagt tcatcatgcg tggagatgtc ttggatcttg ctgtcggcgt tgttattggt
     6061 agtgcgttta caagcatcgt aaaccaagtg gtgcaaggct tgatcacacc actaataggt
     6121 tggtttgtcg caatgatcac aggtacttct gatttagaag gagcactttc aattcttgac
     6181 tggtcgccga caaaaggcgt cacatttgcg tttggtaacg ttattagtgc tatcattaca
     6241 tttctgatca ctggttttgt cttattctta gttgtcaaag cggcaaaccg tgcgaagaat
     6301 ttacgctcca aagaagagca agtggaagtg gaagaaactc ctactgcaga agattatctt
     6361 agtgacatac gcgatcttct tgccaaacaa gtggcagaag acaatacaca tccagtagag
     6421 caaacaaatc tttccgatac ttctaaataa taaagaatct aaaaaaaaga aagccgattt
     6481 tcagtgtcgg ctttcttttt ttagattctt aacttgctac ttgttccttt ttcatgtcga
     6541 tatgtggaat atcatcttcc aaatacacat ctgagattgc cttgaatcca aacgactcat
     6601 aaaaagcttg taggtattct tgtgcgccga tcacgattgg tttttctgga aaatttttct
     6661 ggatcatgtt aatcgtttct tgaacaatct ttcttcctaa accatcttta cggttgtcct
     6721 gatgaacaag gacacgtcca aaatggatac tctccgcttc ttcatatact cgcgtgtagg
     6781 caaggatctt cccatcagta tcttgaaaaa acgtatgcca agcttgtcca tcaatgtcat
     6841 caacttcttg ataaggacac tgttgttcca caacaaaaac tgcgattcgt tctttgacga
     6901 tcgcgtaaaa ctctgctgtt gacaattctt caaatttctt tacctgatat tccactaagc
     6961 aaacgccccc tgctttttta ttcttagcat accgattcgc acacaaaaaa gcaaacgaac
     7021 cttacgaat
//
LOCUS       DANKKG010000129         6872 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000129 DANKKG010000000
VERSION     DANKKG010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6872
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.129"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(3..461)
                     /locus_tag="PW004_002334"
     CDS             complement(3..461)
                     /locus_tag="PW004_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2727284.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(474..1805)
                     /locus_tag="PW004_002335"
     CDS             complement(474..1805)
                     /locus_tag="PW004_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HDL2727285.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1816..3258)
                     /locus_tag="PW004_002336"
     CDS             complement(1816..3258)
                     /locus_tag="PW004_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2727286.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3245..4009)
                     /locus_tag="PW004_002337"
     CDS             complement(3245..4009)
                     /locus_tag="PW004_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HDL2727287.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4113..4988)
                     /locus_tag="PW004_002338"
     CDS             complement(4113..4988)
                     /locus_tag="PW004_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL2727288.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5243..5327)
                     /locus_tag="PW004_002339"
     tRNA            complement(5243..5327)
                     /locus_tag="PW004_002339"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5291..5293),aa:Leu,seq:caa)
     gene            complement(5345..5416)
                     /locus_tag="PW004_002340"
     tRNA            complement(5345..5416)
                     /locus_tag="PW004_002340"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5382..5384),aa:Gln,seq:ttg)
     gene            complement(5426..5498)
                     /locus_tag="PW004_002341"
     tRNA            complement(5426..5498)
                     /locus_tag="PW004_002341"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5463..5465),aa:His,seq:gtg)
     gene            complement(5560..5644)
                     /locus_tag="PW004_002342"
     tRNA            complement(5560..5644)
                     /locus_tag="PW004_002342"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5608..5610),aa:Leu,seq:caa)
     gene            complement(5665..5735)
                     /locus_tag="PW004_002343"
     tRNA            complement(5665..5735)
                     /locus_tag="PW004_002343"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5701..5703),aa:Cys,seq:gca)
     gene            complement(5781..5852)
                     /locus_tag="PW004_002344"
     tRNA            complement(5781..5852)
                     /locus_tag="PW004_002344"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5818..5820),aa:Gln,seq:ttg)
     gene            complement(5864..5936)
                     /locus_tag="PW004_002345"
     tRNA            complement(5864..5936)
                     /locus_tag="PW004_002345"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5901..5903),aa:His,seq:gtg)
     gene            complement(5939..6012)
                     /locus_tag="PW004_002346"
     tRNA            complement(5939..6012)
                     /locus_tag="PW004_002346"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5978..5980),aa:Trp,seq:cca)
     gene            complement(6019..6099)
                     /locus_tag="PW004_002347"
     tRNA            complement(6019..6099)
                     /locus_tag="PW004_002347"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6063..6065),aa:Tyr,seq:gta)
     gene            complement(6101..6176)
                     /locus_tag="PW004_002348"
     tRNA            complement(6101..6176)
                     /locus_tag="PW004_002348"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6141..6143),aa:Phe,seq:gaa)
     gene            complement(6212..6284)
                     /locus_tag="PW004_002349"
     tRNA            complement(6212..6284)
                     /locus_tag="PW004_002349"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6249..6251),aa:Asp,seq:gtc)
     gene            complement(6294..6366)
                     /locus_tag="PW004_002350"
     tRNA            complement(6294..6366)
                     /locus_tag="PW004_002350"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6331..6333),aa:Val,seq:tac)
     gene            complement(6382..6453)
                     /locus_tag="PW004_002351"
     tRNA            complement(6382..6453)
                     /locus_tag="PW004_002351"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6418..6420),aa:Glu,seq:ttc)
     gene            complement(6468..6557)
                     /locus_tag="PW004_002352"
     tRNA            complement(6468..6557)
                     /locus_tag="PW004_002352"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6521..6523),aa:Ser,seq:gga)
     gene            complement(6597..6670)
                     /locus_tag="PW004_002353"
     tRNA            complement(6597..6670)
                     /locus_tag="PW004_002353"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6634..6636),aa:Asn,seq:gtt)
     gene            complement(6680..6795)
                     /gene="rrf"
                     /locus_tag="PW004_002354"
     rRNA            complement(6680..6795)
                     /gene="rrf"
                     /locus_tag="PW004_002354"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         2048 a         1529 c         1138 g         2157 t
ORIGIN      
        1 cactaaaatt caaaacaact atctttaatc tttacttctt ggttttgttt taataaattt
       61 ccttcaaaaa tgcctggaga aactacaatg atcaccgtat ctttttgtgc ttgctccaac
      121 ttcttcaaat ctataatagc taacagtgtc ccttctttta cctcctggtt actttcaacc
      181 cgtatctgaa acggacttcc ttcgagttca actgtatcta aacccatatg gatcagtatt
      241 tttctgccag attcactttc gatcgttatg gcatgtcggg taggaaatat attatcgatt
      301 attcctttaa ctggcgcatg gatatatcca tcatgattcg ttatagcgta tccaactcct
      361 atcatttttg aagaaaagac ctcgtctttt actgctgtta atggaataat ttgaccacta
      421 gctacagctt ttagaatgtt tcttgtttta aagaaatgca ctttttatca cctctacttt
      481 aatgctggcc aatatttttt gtttactttg atcatttcat ctagtatttt ttttgcgact
      541 ctggcatttg gtacggtttg attcaatgta aatgcttgta aagctttttg ataagaacct
      601 tcaaagtaag catctactag taacttctct actgcaactt gtgcctccat caatccttta
      661 tgaaaatcac tgattggttt tcttagtgta attggctcca caccagttgc accaacatat
      721 gccggtattt caacaactgc atctgaccgt agattcggaa tcgccccttg attaggaacg
      781 attagcataa agcgacgacg atcatcattc aacaaggaaa tagctatatc gacgatatat
      841 tgtccatgct ccccaaaatt aaagttcaat acttcttcca caataccgcg gcgtatttta
      901 tcagccattt ctttggtatt tttctctcgt ccatccatga ccatattagc acgtgtatac
      961 tctttgtcag tatgctcaac gaccatatca ggatataagt aatactctaa gtaattattc
     1021 gggatattac ttggaaaatt tttcgtgatg actgacatca tttgaaaggt tttttgccaa
     1081 gttttatctc ctatattaaa atcggcgact tgcatttctt ttgttattag tttttcaata
     1141 atctctggca taatttctcg ttgcaattct ttatcataaa tcgatgtata ccagccaaag
     1201 tggtttaaac cataatacgt aacgatccaa ttcttacgat cataaccgta attcactgca
     1261 atcgtttctt caattgaaat agtcatatca caggcattaa tcatttttac attcggatat
     1321 ttacgacgaa cagcttcaga aacgatcgac tctggatttg tataattcaa aatccatgct
     1381 ttgggggcat agtcttgtat atgatcgaca atttccaata aacctttgat tgagcgcatc
     1441 ccataagcaa atcctccgag tccacacgtt tcttgtccta ctaatccatg tttaagtgga
     1501 atcttttcgt ccttttctcg catctccaat cctccaacac gaatttgtga gaaaacaaaa
     1561 tcgcatcctg taaaagcaag ctttggatca tccgttgcac gaatgcgaat atttgaaaaa
     1621 ccttttcttt tcagcataaa tttaacaatc aagtacatat ccatatttct ttcttcatca
     1681 atatcatata aacgtatttc agataatggc aaccgttttt cattgttcaa aatggcttga
     1741 acgattcctg gtgtatacgt actcccgcct ccagctatag tgataatctg ttttttcatc
     1801 atcatctttt cctttctatt cttctaataa tttattgaat tctgccataa cataagtcac
     1861 ttctaagccg atgataattt gataattttt ttcagttggt ttaatgatcc cgcttatccc
     1921 taattgcttg attcgctgtt cctcgaccat ggtgggatcc actacctcta cccgtaggcg
     1981 tgtattacaa ctatagacat ccaggatatt ggcttttcct ccaaacgctt gtagcatttt
     2041 tgttgccata tattcataat ttttacttgc taaccctaac tctgtttctt ctaaggttac
     2101 ttcattgcca aactctgcct tagcttcacg tcctaatgtc tgaatgtttc tcttattgat
     2161 caaataataa aaagtaacgt aatacaaggc aaaaaagaaa attccgattg gaagaatcaa
     2221 aatagcattg gtagcgattc gaaaattgat gagataatca acaatacatg ctccgaatgt
     2281 aaaaccaatt cgtatattga ataagtaaca gacaatccct gctaaaccgg caaaaagtgc
     2341 atggatcacg tacaacatag gagaaagaaa catgaaacta aattcaacag gctcagtgat
     2401 atttgcaata aatgaagttc ctgcaccgct tcccatgatt cctttgacca tctctttatt
     2461 ttctttataa gctgctttat agatagctag acatatagct ggtataccaa acatcattac
     2521 tacaaaaaaa actgataaaa acagtccagc ctgaggatct ccgttgataa agcgtgtcat
     2581 ctctcctctg aaaacttcct tgctaggaga tgtatagctt cctaaatcgt aataaatata
     2641 cgtattcaac acatggtgca gtccaaaggg gatcagcaaa cgattcaaaa acataaatat
     2701 ccccacacct aatgctccca tatcaacgag cagttttgca aaattatcaa tcccaatttg
     2761 gatgagcggc cagataaagc ccaaaaaaac agctgcgatt gtttgtagaa tcatcaccat
     2821 agtcaatggg aatttttcgc ccgcaaaata agaaaaaact actggtaatt tttgttcttt
     2881 aaagcgatta tacgtccatg ccgctactaa accggaaata attccaccaa aaatccccat
     2941 attcacttct gggtccataa tttctaagcc ttttttcaag gtagcaatcg ctaaaaaacc
     3001 agtaagtgca ggtattcctt tgtccttatt tttgacaaaa ccgattgcaa ctcctatagc
     3061 aaataagata tccaagtttc caaaaacggc atttccagct gcaccaatta ctgggatgtt
     3121 cagcatatcg tcagctgata aacgcatgat caatcccgct aatggcatcg ctgcaactgg
     3181 aagtagcatc gctcttccaa gcttctgcac atttgattta acattcattt ttattcctcc
     3241 tctattaaat atcctcatat gttactaact caatcaaagg atcatttttc actttttgag
     3301 caaatgctga atgaattaaa aattcgactt ggtgggtacg aggatacaca aaacttgaac
     3361 catttaacaa ttcctgatcc aaatacgctg cgtgacacat gacttcgatc gttttatctt
     3421 ctctcaatat catgagtaaa cttgccaaat attcttccac actcgtattg atttgtcgtt
     3481 ttttttcatc agtttctcct acgacatcga aacaaggctc aaaataatcg acatgacgaa
     3541 cttcatcttt tcgttcaaag ttgctccgaa ccggcaaatc atattttttt gctaatgtaa
     3601 caactacctc ctgattttca tccaaagtat gagtatgatg atgactgtct aaatgagttg
     3661 gtatgatccc cgcatgataa attttttgaa tctgagcatt ccattcctga tacagttgat
     3721 ctttgtcaat gttaaatgga tgctgataat aagataagga tctgaactct ccgctttcga
     3781 taagagtatc gacagttttg aggagtggtt tgccacacgt caatgttaaa tggactccta
     3841 cacctagcct aggcatttcc tttcttagtt tcacagcatg ctcgaaaccc ggcatatttg
     3901 ccatcaatgt agttgatgtc aaaattcctc gctgataagc atccataatc ccataattca
     3961 caccatggct atagccaaaa tcatccgaat taaaaatcac ttttcccatt tcattctcct
     4021 tcttttttct actaaaatag gcgcaaccaa ataatcatag tccatttact atgtgtcatt
     4081 tggttgcgcc tatagatagt tacgccctta ttttaataga ttcttgctag atgaattgcc
     4141 aaatagatga tttcattttc ttgaatggca agttgaaatt gctgttcgat gatatcttta
     4201 atctttagtg ctgtacaata cgctttgcta tgtttaaatg tttcagtaaa aaaaccaata
     4261 ttttctactg gcgtttcttc ctgataaatc aagcgtttaa ccaggaattt gatatgaacc
     4321 attaagcgat gataagtcag tgattcttta tcataaacgt ttgaatagga tttttctaga
     4381 tgggacaaaa tcgtggtcgt catttctact atttttaagc tctttaaact attaggctct
     4441 cctagctcag cattaatgat atgcatagtg atgaatccta cttcatttga ccctaactcg
     4501 gtttgatatc gatgattgat tcgctcgacc acttctactg ctatatcata ttcatttgga
     4561 taaaggaatc tcatttctgg caacataatt gctgtgacat ctctatcttc tctttttcgt
     4621 tgaatagcaa aataaatatg atctgtcaat gtaatataga tatttgaatc taatggagag
     4681 gagatcctag ctgttatcag ttcaatcact tcttccgcaa aatccataaa ttcgattgga
     4741 atctgcgaga ctatctcact gaatcgtttg atcttttctt tagaatctaa tacaaataat
     4801 ttgtcagcat cctcaacatc aaatggctta ctcactttag cagaaaagcc aatccctttc
     4861 cccatcgcaa ttttttctat tccatcgtca tcgatcaata cagcattatg attcagcttt
     4921 ttcatcacat acaccgttaa ttcacctcct ctatatgata aaacaaaaca acttgcagac
     4981 tccttcatac atatttttgt ctccaaacaa tttttaaaga caaaaaacga cctaagaaag
     5041 aaccctttct taggtcgtgc ctaattgaat agtagcaccc tacataattt acaaaaatag
     5101 tttaacacat aataaaagcg ttatcaatat cttttttact cgttttatga taatacttgt
     5161 atcgaattga tttggaggta attacataaa aacaaaaaaa atatagcaac tctctataag
     5221 aaagttgcta tattgataat tataccggcg gccggggtcg aaccggcacg tcctcaacgg
     5281 acactggatt ttgagtccag cgcgtctgcc aattccgcca cgccggcaaa tttaaaaata
     5341 ataactgggg tagctggatt cgaaccaacg catgagggag tcaaagtccc ttgccttacc
     5401 gcttggctat accccaagaa taaagggcga atgatgggaa tcgaacccac gaatgccgga
     5461 gccacaatcc ggtgcgttaa ccacttcgcc acattcgcca tcataaaaga tgttatttaa
     5521 tttctcaata aaaaactaag atgaagacat cttagtcaat accggcggcc ggggtcgaac
     5581 cggcacgccc tcaacgggca ctggattttg agtccagcgc gtctgccaat tccgccacgc
     5641 cggcaagttg atttattagt actaaggcgg taaccggatt tgaaccggtg atgaaggttt
     5701 tgcagacctc tgccttacca cttggctata ccgccattat actattatca atctataaaa
     5761 taaagaataa attatagaaa ctggggtagc tggattcgaa ccaacgcatg agggagtcaa
     5821 agtcccttgc cttaccgctt ggctataccc caagaataaa aagggcgaat gatgggaatc
     5881 gaacccacga atgccggagc cacaatccgg tgcgttaacc acttcgccac attcgccatg
     5941 gcaggggcag caggaattga acccacacta acggttttgg agaccgctgt tctaccttta
     6001 aactatgccc ctataaaatg gaggagagtg gattcgaacc actgaaccct aaggaacgga
     6061 tttacagtcc gtcgcgttta gccacttcgc tactcctcca tggtggcgcg ggacagaatc
     6121 gaactgccga cacacggagc ttcaatccgt tgctctacca actgagctac cgcgccatat
     6181 tttaaaatta ttaattgaat aacaagaacg acggttccga cgggaatcga acccgcgatc
     6241 tcctgcgtga caggcaggca tgttaacccc tacaccacgg aaccagttca ttatggaggt
     6301 taacgggatc gaaccgctga ccctctgctt gtaaggcaga tgctctccca gctgagctaa
     6361 acctccaaca ttcaattaaa atgacccgta cgggattcga acccgtgtta ccgccgtgaa
     6421 agggcggtgt cttaaccgct tgaccaacgg gccaggtaaa tttgctacgg agagtaaggg
     6481 attcgaaccc ttgagacagt ttggcaccat ctacatgatt tccaatcatg ctccttcggc
     6541 ctctcggaca actctccaga gtgaaagaag ctcaatactc tttctcaaaa tgagaactcc
     6601 ggcagtagga ctcgaaccta cgacatcatg attaacagtc atgcgctact accaactgag
     6661 ctatgccgga ataacatcag cgtggcgacg tcctactctc acaaggggca acccctcact
     6721 acaatcggcg ctaagaagct taacttctgt gttcggcatg gttacaggtg tatccttctc
     6781 gctatcgcca ccacactgtg gtgttatctt ttattgagta aattttgttc actcaaaact
     6841 ggatttgaag tcacataagt ttttttccga gt
//
LOCUS       DANKKG010000130         6815 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000130 DANKKG010000000
VERSION     DANKKG010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.130"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            183..539
                     /locus_tag="PW004_002355"
     CDS             183..539
                     /locus_tag="PW004_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HDL2727289.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            549..1448
                     /locus_tag="PW004_002356"
     CDS             549..1448
                     /locus_tag="PW004_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HDL2727290.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            1965..2696
                     /locus_tag="PW004_002357"
     CDS             1965..2696
                     /locus_tag="PW004_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HDL2727291.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            2841..4868
                     /locus_tag="PW004_002358"
     CDS             2841..4868
                     /locus_tag="PW004_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HDL2727292.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            4976..6073
                     /locus_tag="PW004_002359"
     CDS             4976..6073
                     /locus_tag="PW004_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HDL2727293.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            6380..>6815
                     /locus_tag="PW004_002360"
     CDS             6380..>6815
                     /locus_tag="PW004_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2727294.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFE"
BASE COUNT         2393 a         1101 c         1381 g         1940 t
ORIGIN      
        1 tttttgggtc tttttccgtt caagaaactg aacgaagaaa agggaaagtt gtctcttttg
       61 tgataagaga aatcataata aacgaatgac ttactttcca ataaattgag aacagtcaga
      121 accagacaaa ttagttgcat ctgatgaata aagtgattat tctatgacat gaggtgatac
      181 agatgaaatt agaagtgaca aaagaagcac aagaagtatt gaagaataaa ttagaagcag
      241 acgatcaact tttattagat atcgaaaacg gtgacggtcc atttgcagaa agccaagtca
      301 gttgccaatt ggatacggca tttcgattga tcattgtcaa aaaagataca caaacagatc
      361 taagtcttta ttctgtagtg gctgatacac cagttggacc aatcaaaatc aaagacagtg
      421 caatacttta catggatgat ccatccacac ttgctttaga accaacttat aatagtttac
      481 aattaaaagg accaagcggc ttgagaaaag gcaacttgca agttgttcga aaagactaga
      541 gggataaaat gggaaaattg gcgatcatca gcgacctaca tgtggacatc aataaattcg
      601 gcgaatcaga gctgatgcag ctttggcaag ttttgcaaaa gcacaagatc actcggcttc
      661 atctagccgg agatacggct aataaagtag accgttgcct agctgttgtc gaatttttcg
      721 tcaataaagg attatctacg acatttcatt tcggcaatca cgagttggca gatgtgccca
      781 atgaacagat gatggaccat tttccagacc cacatttttt aaatgaacgg tatattgctt
      841 taaatgagca gaccgttctt ttagggatga atggctggta cgactatcag ttttccgaat
      901 tgaaagatta cgaggcaatc gtacgaatga agcgtttgta ttggtatgat cggttcatcc
      961 agcgtgggaa aacagatctc gaggtcaatg cagaaatgct tgcagctgca aaaaatttgc
     1021 tagaccagct ggaagcaaaa aaacttgagg tgattttatc cactcatttc gttcctaaac
     1081 gggcatttat catgtatcaa tctgctcctt atgagcgttg gaacaaatta aatgcatttt
     1141 taggatcaga atcattcgga gaattacttg accgttattc aaatatcaaa caagtagttt
     1201 ttggccatac gcatcgtcgc tttgaagata aatccatcgg tcacacgatt tacagctgca
     1261 gaccttttgg ttattattat gaatggcagc tgacccgaga ttttgtcttg aaagaacagt
     1321 tgctagacag ctatgatcct atgaaattac gtgtcctctt gcgtgcccat cacccagctt
     1381 ttcaggccta tcaaacacaa catttgcaag aggaatttga acaagcgatg acaatcgttc
     1441 cgtattgaga cagcattagt aatgtgtaaa agaaaatacc ttgtaaaaca taaggataag
     1501 taagagcagt agatccattt atcgtggata tgctgctttt tttgcggaaa ataatcagcg
     1561 aatgtttatt tgatcggatg atttgtaact cgcagagtac tagatgcaag cgtgtattct
     1621 ttttttgaac gataatcaca aaaaaaaata taatatacat tttttattta attataatta
     1681 atattgattg aagtctaggc agtcaagtca cattttgata gataggaggg ggacttacga
     1741 attattttaa aggctatgac agctgtattt ctagattgtg tttgcatcaa gttgcaataa
     1801 aaactagaaa tcaaatcggg ctgataatca tttataacta atcgatacat aggaccaaat
     1861 ataggtctat gttcttttat ttcataagta tcgtaaaaca gaattttgct atggattaat
     1921 ccaatcgact gatatgttta gtaaataaag aaagaggaat gtcaatgatg aaaaaagaaa
     1981 tcgtattaac agcagcaatg atgttattca gcatggtagc aagtacgact tttgtttctg
     2041 caactgaagt atacccaaag gaatataaca cagaaggaac gattacattt gaagcaggag
     2101 atgagggtgt aactcctcct gttgatccag agaatcctga tcccaataaa cctgtcgatc
     2161 catcagatcc tccaagccct ggaacaggag gggcattatc gattgattat ggatcaaaat
     2221 tcaaatttgg gactcaaaaa atttctactg cggataaaac gtattatgca gctgctgatg
     2281 tgatgaacga tgggtctaga aaaccgacat atgtccaagt gacagatagg agaagtacct
     2341 tatctgggtg gaaattaagt gtctctcaac cagaacagtt taaaactgcc agtggggatg
     2401 aactagttgg agcacagctg aaatttacga aagggcaggc tgtttctttg gttgatccaa
     2461 catatacgcc gcaaacggtg aattctgaat taacgttgac accgggagga aacaatacgt
     2521 tagcaattaa tgctaaatca ggaacaggtg tggggacttg ggtctatcga tttggtgcaa
     2581 atgaaaatga aaatcaagat gcggtacagc tttcggtacc cggtaaatct gtcaagttag
     2641 cgcaacaata ttccactaaa ttagtttgga cattagaaga tacacctaac aactagttta
     2701 ctgaaaagaa ctgttaaaaa taaatacgta gtcatattgt cttgcgaggt aatcgagcaa
     2761 aaattgtcaa aggctaggct aaatttgttt ggatacgaat agttcatcta ggaatttaac
     2821 gatcaggagg aaaagatata ttgaaaaaga ggaggatcgt gctcatttta agcagtctct
     2881 tagtgactaa tatctttccg atcatagggt actctttgga attgaatcag ttggagcatg
     2941 gaaatagaag agacgaagaa agcaaactca cttcgacatt cgacagttta atagaatcca
     3001 accagacaca agaaagcagt gaggatttaa gtatttcgag tagtggagag accactgaca
     3061 acgtagaagc tgagggttcg gacaaacaag gactagaaga tgcaccaatt tctgcggttg
     3121 agctgcctga cgatcccaat attatttcta tcgataaaat ttttcaaaca cctatcggta
     3181 ctagcacgtc catattggaa aatgggaagt tactgcagtt gaacccagca gaaaggtctc
     3241 aaagaggagc tatttggtca aataagaaga ttagtttact ctcggatttt acatttaaat
     3301 cttatttata cttaggaaat caacgagata gagcgggaga tggcatgacc tttacattga
     3361 ctggcgatcc tagaatggct agtaatccca gtcaagttat tggatcgcca ggtatgggaa
     3421 ttggctcata ttctactcgt tctggccaac cttacattgc gaatgcgctc tcgattgagt
     3481 ttgatacgta taaaaatagt ggttcttctg atcgaatgga tcgagagata actgctgata
     3541 atagagggca tggacatgtc gcttttgtca cgcctaaacc aaataataat aattattctg
     3601 gagagcatac tggcgtgacg gtagctccta catttctttc taatggcaca tggcgcttgt
     3661 taataattaa atgggacgcg gagcaaagaa gtcttactta tgatttggaa ggtgtcggtc
     3721 gctcttctcg gattatacct gatctcatta ggcagtttgg ttctacagaa gtaaattggg
     3781 ggtttacttc ttcaacaggt actttttttc aagaaaatgc tgtcgctgtc acgcgacttc
     3841 catcgaatgt cacatcaaca gctgatgtaa aagtcaacga tagtgattat ggggccatcg
     3901 ctgaagctgg aggaaacgat aagataacta tcagaaatac attgaaggtc gagggggatt
     3961 ttcttgaata taagaaaatg cagactagta tcgacctccc acaagaatta actattcccg
     4021 agcatatctt gatcaatgga aatcaaattg atgccaaaga tatcttgata accggaaatc
     4081 gagtcacgct aaatctgaca gattatttta ttgctggaga tacgttaata atcgaattgg
     4141 agacaacttt gcaggaagta attcctgaaa aagtactcac tatgcatttt gaataccttg
     4201 aaaacgggaa tttgattgag caatcgaatg aaataaacct aaagatcgca aagctgaagg
     4261 agatagatat ccatgtttat tataaagata aagacagtca gattgatatt gctgaaatga
     4321 aaacaatcac tgggaaaata ggagagaagt ataaagaaac tcctattgat attgaagggt
     4381 atgtttttga ctcggattca gcgaatacag aaggcatctt ctccgaaaat acagaggata
     4441 tctattttta ttacagaaaa ggcaaacttt atctctcaac tgctcctcaa tatgtcaatt
     4501 ttggaaaaca taaaatatcc agtagaccat taagtgaatt cggacagttt tcaggtgggt
     4561 tgaaaattat agatgaacgg gcttttggaa gttggaaatt acaattgaaa caagagaaag
     4621 ttcttacaaa tggagatttt gagcttccag aagctctttc ctttgttacc tctgaaaata
     4681 caacggttat agggacatcc gctgttacga ttttcgaaag tgatcaaaag ggtgaaagcg
     4741 atttgtcctc tcttttagat tctacagctc atcgtggaat cagaataaat attcctgtag
     4801 aaaaccaaag aatcggaacc tttgagggga aactatcttg gatacttgca gatgtcccag
     4861 gaaattaaca ggaagagaag aatataacaa aattattgag tttcaagaag ggcacaggta
     4921 ccataggctc ttgtgtccca cagagacaag attttgttat agtcttctct tacatatgat
     4981 taaaggagcg attttcttga gaccaaaaca acaattattt ataacactcc tagttatttt
     5041 aggggcactt tttaacaata tatcagtgtc agctagtgca tttaattttt cagtgacgcc
     5101 gataacttca gagaatcaaa tcgacaagag aaaaacttac ttcgatcttc aacttgatcc
     5161 ggatcaggaa gtagaagtaa aagcggaatt gagaaatgat acagaaaaag aagtgaaaat
     5221 cgatatttct gtgaatagcg caaccaccaa ttcaaatgtg atggttgaat acggaaaaaa
     5281 cgagattgaa aaagacatga gcctgatctt tgatctggta gattatgtga gctatccaca
     5341 gatagtgacg ctaaaaccta aaagtgtcca aaccgttgct tttcatgtca ggatgccgaa
     5401 tgagcgattt gatggtgtat tggctggtgg gatcacattc caagaaatcc agacggaaaa
     5461 ggaccaaaca gaaactaagg atcaaagctt atcgattgag aatgaataca cctatattat
     5521 tgctttgttg atgcaacaga cactgaacga agttgctccg aaccttactc ttcatgaagt
     5581 taaaccagat caaatcaatg caagaaatgt catcttagca aacgttcaaa atgatcaaaa
     5641 aacctatatc aatcaagtag ttattgaaac aaaaattaca aaaaaaggac attctgaagt
     5701 tttatatcaa gaggaaaaag aaggattgca gattgcgccg aatacaaatt tttcttttcc
     5761 aactgcttta aatggtcaac cgctaacccc aggtgaatat caccttacaa tgactgtttt
     5821 aggaaatgaa aatgaaaatg gaaaattttc gagaaaaaaa ggaaatacta cgatcaacta
     5881 taccaatcaa tgggtatttg aaaaagattt tacaatagac ggaaaagtag ccaaggaatt
     5941 gaacacgacg gatgttacga ttaaaaaaga caataattgg atttatatct tagtagcttc
     6001 acttttctta ttgtttattt tattatgcat acttttgttt gttttgagaa aaaaagaaga
     6061 gaaagaaaag taaggttaag ataacaaata gtaaattttt attattgtag gatggttggg
     6121 ctctttttat aacaatataa aatataccat tttattaata aaaatgcata ctagtaaatg
     6181 atataattct aataacaggt ttgatcgcga gaaaggccaa ataaaacgct ctccaaaatg
     6241 ttaaaaaaaa gacaggacga tgtatattat acaaacaaga atcgctgaca acagtgactc
     6301 ggatccagga aatcagtttt atccccagct agttggtgaa tgcatggaaa gtaatatacg
     6361 tagaaagagc tgatgactaa tgaattatga gtttttttta agcaaagaag atcacagaag
     6421 ttattcatta cttaaatatt tagaagcaag ctcaaattta tcagagtcaa tatcgaatgt
     6481 tcaagaagag ttgtcgctat cgacatttct cttgaaaaaa acgatcgata agctgaaaca
     6541 tgatttacaa aagttcgatc tagagaaaaa tttgcgagta accgtcagtg aattggatat
     6601 atgtctggaa attaatggaa aatgttctag caaagctctg ttatctcgtt atataacgga
     6661 ttctttatct atgaaaatgg tattggcttt ctttcaggaa aaatatatct cagtagaaaa
     6721 ttttgctaaa cagcatcacg tgagttattc tgtggcttat aaagtattac aaagactaaa
     6781 aagaaatcta aaaaaatatc aaattttttt tgaaa
//
LOCUS       DANKKG010000131         6698 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000131 DANKKG010000000
VERSION     DANKKG010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6698
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.131"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            146..1018
                     /locus_tag="PW004_002361"
     CDS             146..1018
                     /locus_tag="PW004_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL2727295.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            1015..2973
                     /locus_tag="PW004_002362"
     CDS             1015..2973
                     /locus_tag="PW004_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2727296.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(3224..4075)
                     /locus_tag="PW004_002363"
     CDS             complement(3224..4075)
                     /locus_tag="PW004_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL2727297.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            4198..4665
                     /locus_tag="PW004_002364"
     CDS             4198..4665
                     /locus_tag="PW004_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HDL2727298.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(4928..5410)
                     /locus_tag="PW004_002365"
     CDS             complement(4928..5410)
                     /locus_tag="PW004_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HDL2727299.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(5510..6373)
                     /locus_tag="PW004_002366"
     CDS             complement(5510..6373)
                     /locus_tag="PW004_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDL2727300.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         1986 a         1390 c         1231 g         2091 t
ORIGIN      
        1 cgtttcatgt gactagatca tactaggcat gaaaaatctc tgaggaacat ggtttcccta
       61 ggaggttttt cgtgtctatt tttgtttggc cgcaatcaac tggtctagca aacgtgaaaa
      121 ccaaatcttc aaaaagggtg cgcgcatgaa aatcaaaaag atctttaatc aaaatgcagt
      181 attggtagat gacggtggac aggaaaaagt agctattgga aaagggattg gatttgacag
      241 aaaaagaaat gatttgattt ttgatcgtga tgtcgaacgg atatttgtca tggaaccaga
      301 aggtcaaatc aagttgcaaa atttacttaa tcagattgat gaacgctttt tatttgcagc
      361 agaaacgatc attgatcatg cggaaactgt tttgatggaa aagttgaatg aacatgtatt
      421 gatcgctttg accgaccaca ttgctttttc tgcggagaac attaataacg gtatcgtcat
      481 ccgcaacaaa cttttaagag aaatcgaagt gctttacagt gatgaattca gtattgctca
      541 atgggcagta gagtatctga caaaagagct tgggattcca tatacttatg acgaagcagg
      601 atatatcgca atccatattc acagcggacg aagcggtcgg aacaataacc atcgcagtat
      661 ccgtgaagtg acgattgtat ctgacatcat ccatttagtg gaatcagaat tggaaacaga
      721 tatccattca gaaccatttt cactgaatta ttcaagactg gtgaatcatt tacgactgct
      781 tctacaacga acccatgcgc aacaatatgc tgttttagac acggaaatcg tagaaatggt
      841 caaacgaaaa tacccagaaa gctataagat ttctaaaaaa atccgtgtgt tgctgaccaa
      901 agaataccag ctcgcgatca cgaaagaaga actgggttac ttggccatcc atatcgagcg
      961 tttaagaagc gcaatcgtac aaacaaatgt taacaatcat gaggaggaaa aaaattgaaa
     1021 gcatatctac aacgaatggg acgttccctt caacttccag ttgcggtctt acctgctgct
     1081 gctttactag tagggatcgg aaactggtgg gcatcttata gtaacgacat tgtcgctcat
     1141 tttttacaag ctggcggtaa cgcagtttta ggtcaattac ctttgctgtt tgccgttggg
     1201 ttagcactag ggatgtcagt ggacaaatca ggtgctgctg ccttggcagg tttggtcgca
     1261 tttgaagttc cagtaaatgt tttaaaaact gattcagtag ctactttatt gaatatcaaa
     1321 ccagaatcag ttgatccatc ttttgctcag atcagtaacg tatttattgg tattattgcc
     1381 ggattgattg cggcagctct ttataatcgt ttccacgaag caaaattacc gatggcttta
     1441 tctttcttta gcggcaagcg tctagtgcca atcttggctg cctttgtcat gttgctgatc
     1501 agtgcagtgc tactgctcgt atggcctccg gtttactctg ctttagtttc ttttgctaaa
     1561 ttcattttga gtttaggctg gggtggtgca ggtctttatg gcttcttcaa ccgtttactg
     1621 atccctacag gattgcatca agcaatgaac tccgtgttct ggtttgacgt agcaggaatc
     1681 aatgatattg gtaaattttt ggcttctgaa ggaacaaaag gcgtaacagg aatgtatcaa
     1741 gctggatttt tcccaatcat gatgttcggg cttccagcag gagcatatgc tatttacaga
     1801 aatgcacgtc cagaaaacaa agcaaaaaca gcttccttga tgctagcagc agcttttgct
     1861 tcattcttta ctggtgtgac agaaccactt gaattttcat ttatgttcgt tgcttggcca
     1921 ttatatgtct tgcatgcaat tttcactggg atttctttag cagtgagtgc attcttccat
     1981 tggacagcag gatttgcctt tagtgccgga tttgtcgatt acttcctatc tttgaaaaat
     2041 ccgattgcga atcagccatt gatgttgatc gtccaaggat tagtaacagc agtgatttac
     2101 tactttggct ttaactttgc tatcaaaaaa ttccatctga tgactcctgg ccgcgaggaa
     2161 gcagatcttt cagatgacga tactgcaacg actaattcag acaataaata tgcggcacaa
     2221 gcaagcaaaa tctacgctgc attaggcgga gccgacaatg taacttcgat cgataactgt
     2281 actacacgcc ttcgtttgca agtcaaagat acaggcctga tcgatcaaaa taaaatcaaa
     2341 gccactggcg tacctggtat gaagatcatc gacggaaaaa atgcgcaagt catcgtaggt
     2401 acagaagtac aattcgttgc agatgagatg gcaaaactac atggtggtgc tgcagcacga
     2461 cctgcacaaa caaacaccgt ggttaaaaca gaaacatttg ccggtgaaac gatcgaacaa
     2521 gatatttacg ctatcgcaaa tgggaaactg attccaatca ctgaagtttc agatgatgta
     2581 ttcgcggaaa aaatgatggg tgacggctat gctgtattac cagaaaatgg agaaatcttc
     2641 tctccaatcg ctggtacgat cacaaacatt ttcccaacga aacatgctgt cggaatccaa
     2701 acagatgctg gtatcgaagt cttgcttcat atgggcatca acactgttga cttaaaaggc
     2761 gaaccattca ctttatatgt agaagaaggt caaaaagtcg caagaggtca attgattgct
     2821 ttagttgatc ttgccgctat tcaatctgca ggcaaaaaca cggatatggt cgttgtattc
     2881 acaaatggcg acaaagtcca aagcttagaa atcgaacccg cacgtgatgt aaaagcaaat
     2941 gataaaatcg gctctgtttc caacaaagcc tgagccatcg tcttccccag acttttttat
     3001 cgataaacaa acaggagctg agacaaaaat cttgtctcag ctcctttttc cgaataaaaa
     3061 cggcgttctc ctgccaactc cgcggtaata agtccagcta aacgaaacat atgaagagct
     3121 attttttatt tgactcttct tattacttga tgcagaacag ctttttcaca aaatttttct
     3181 tcatactcaa aactaatcgg ctttttcaca acctctttat acattattcc tgatccaata
     3241 tttcttttaa tgctgcttca agagaagtta atggttttcc caatactttt tctaaatcat
     3301 cagaggaaat agctaactga tcctttttaa tatcatgttg gatcgctaaa aacatgtcag
     3361 ctgcttcttg agggaatccg gcttctttta gattttcgac aaacgctgca tcacttgctt
     3421 caataatttc taactcttta cctgtcgcac gctctaatgc cttggctaat tcttcatagt
     3481 cagttagatt gccggataat tctaagattt ctggagagtc actttctact agtacttttg
     3541 ctgcagcttc tgcatattca cgtttcaatg cccaacctgt ttttccattt cctgcagcat
     3601 aaacgaattg accagttttt aaagcctgcc caatgatagg taattcattc tctacatacc
     3661 aattattccg taaaaatgta tgggaaatac ctgaatcctt aatcattttt tcagtgaatt
     3721 ggtgatctgg cgctaataca ctgatcgagt gatcggcatc tgcaaaactt gtataagcga
     3781 tataagatac cccagctgct tgtgctgcct tcactacatt cttatgttcg gcttgacgat
     3841 ttcctggaac gccagagaca aaaagcaatc gatctattcc tttcaacgct tctttcattg
     3901 agtctaaatc atcataatca ccaatacgta tattaaatcc agcttctttc agtgcttctc
     3961 ctttttcttc actacgtact agtacataga tttcattttg tggaaccatc ttttttaaga
     4021 attccagcgc ataaccacca aaacctccag ttgctcctgt tactaaataa tgcatatttg
     4081 ccactcccta ttctttttat ttgttaagat ttacagctgt acatttataa attacacctt
     4141 tatcataaag agtgtggtaa gataagtcaa gcaaaaaaaa tgaaaggatg acaactgatg
     4201 aagtattctc tgcaatttag tgatgcaatc catgtacttg cctatattga aatgtttcaa
     4261 gatacccgat cactttctag tgagatgatt gccagtagta tcgaaacaaa tgcagcaaat
     4321 gtacgtaaaa tcatgagtca gctcaaaaaa tccggtttga tctttacaaa aaccggaaaa
     4381 ccgcagcctt cccttgccaa aagtccagat cagatttctt tactagatgt ctatcgcagc
     4441 atcgaaggta atacacagct tatccaagtc gatccaaaaa caaatccaaa ttgcgtcgtt
     4501 ggtgcgaata tccaagaagc tttagaagaa agctacgctc ggctgcagaa aaaggcagaa
     4561 gaagaaatgg ctaagatcac acttgatagt ctgatacata aaatagccgt tttggaaaaa
     4621 gaaaaaagac cagaaaacac aggcctcgta gagagatttt tataattctc tctactgtgc
     4681 ctgcattttt ctgatcggaa tcgtctacat aaattctttt attctccggg ggacaaaagc
     4741 cttgtcatgc ccctttttcc tctctaatgc tcaggtcaaa gcgctgaagt catgacgtct
     4801 tgataaacgg tgttcaagta gctgaccctc ggcattagtt ctgaaaaatg caaaatatgg
     4861 aaagctattt cccatttttc ctctctaatg ctcaggtcaa agcgctactt tcacgacctc
     4921 tatattatca tggtttattg actaccggta tccagatttc tcccatatat ttcccgttgg
     4981 taactcccat ttcgactaca gcatttggac ccccaacata ggcgtaatct tctacttctc
     5041 ttaaaacatg cccaaaggcc gtattcgtca aatcatttct taattcttca ggcgattctg
     5101 ctaaagcgct tacaacaaga tacgttcctt ttgggaatgg gatgattcgc gtagtgtttg
     5161 gaacatcctg actcgtttga acaccgacat agtacatcat tttcccgtta tagggttcgt
     5221 tgactgtaaa gaaataatga ttttcagcag cttgtttcaa tgcatctatt gttccattac
     5281 cgataacttt ttgctcaaat gcttgtttct ctgcagccaa accgacaaaa tccgtattgc
     5341 tactcttcaa ttccacacca atacccgtca ccgtaaaatc tggtttttct tcgatcctat
     5401 actttgccat ttctcccacc ctttctatta tattaagttc atcttacata aaattaaagg
     5461 gaaatgacac gattacgctc ttttttctag ggatattttt attttttaac tatttttttg
     5521 ctttcactgc aatccaaatc tcactgtaaa cattactgaa atcctctccc cattttgtaa
     5581 aagagatctc gggagcttcg actaacccat aatctgaaga aggaagccat tctgcataga
     5641 ttttccccca agtatcttgt aaagtttggg gaaatggccc tttattagga aaaatggccc
     5701 aagtgtgttc ttctatagca agcatttcta aatcagcgta cggattttct tttgtcgtag
     5761 caaagccaat catgtgcatc aaactgccct tttcttccaa gcggtctcca tcaaatccat
     5821 aagaaacgtt gattacttga tttggatata aatctcctaa cttatgcatc tcttcccttt
     5881 gagtctccgt gatcgtctgc gctaactttt gtatttcttg attgactcct tcaaatttga
     5941 caggaacttt tttcgccaca cctgccacat aaaactgttc tttcttttcg atacgaaatt
     6001 ccatcgattt tcctcctctg acatcaataa aaaatgtcaa gcgtggaaaa gatttttgta
     6061 tgccgtatct gctgatttct gaaggcagat atcctgacca atcacgaaaa gctcttgaaa
     6121 aaccttcgac tgtttcatat ccatatctaa acgccgtttc tgtcactctt gatccatcta
     6181 gaagatcgcg attagccaaa gccaatctac ggtttttgat atagtccatt agcgacatcc
     6241 ctgcaataaa cgtaaagatg cgttttaatt gatattcaga gattcctgta ttttcggcta
     6301 ttattttata ggaaagtcct tctgtcaaat gttcttcgat gtaatcgatc aattggttca
     6361 gatctttaag catatttatc ttcctccttg attgcctttt tatcatatta tcaaagaaag
     6421 tcaattactt gatgaatcag gaaccaattt agtcagatgt agaaaatgtt gtatggatct
     6481 aaagaaaatt ctcaagcagc gtttcgtaac ggataattgg tattatctgc ctatctatgt
     6541 tttaccttta gaaaaataaa ttttggggaa gacagattct aagtaaacaa agcggtaaaa
     6601 atatctttta ttcccttagt taatccgctt actacctact gtacaaagca aattgttgtt
     6661 caaactgctg atcagaacgc tgcttggatt tttctttg
//
LOCUS       DANKKG010000132         6614 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000132 DANKKG010000000
VERSION     DANKKG010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.132"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(<1..270)
                     /locus_tag="PW004_002367"
     CDS             complement(<1..270)
                     /locus_tag="PW004_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727301.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRD"
     gene            complement(370..879)
                     /locus_tag="PW004_002368"
     CDS             complement(370..879)
                     /locus_tag="PW004_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HDL2727302.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(964..1443)
                     /gene="rlmH"
                     /locus_tag="PW004_002369"
     CDS             complement(964..1443)
                     /gene="rlmH"
                     /locus_tag="PW004_002369"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HDL2727303.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            1871..3160
                     /locus_tag="PW004_002370"
     CDS             1871..3160
                     /locus_tag="PW004_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDL2727304.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(3199..3804)
                     /locus_tag="PW004_002371"
     CDS             complement(3199..3804)
                     /locus_tag="PW004_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HDL2727305.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(4009..4473)
                     /locus_tag="PW004_002372"
     CDS             complement(4009..4473)
                     /locus_tag="PW004_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL2727306.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(4495..4815)
                     /locus_tag="PW004_002373"
     CDS             complement(4495..4815)
                     /locus_tag="PW004_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDL2727307.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(4959..6038)
                     /gene="pepA"
                     /locus_tag="PW004_002374"
     CDS             complement(4959..6038)
                     /gene="pepA"
                     /locus_tag="PW004_002374"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HDL2727308.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            6238..6588
                     /locus_tag="PW004_002375"
     CDS             6238..6588
                     /locus_tag="PW004_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDL2727309.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
BASE COUNT         1917 a         1350 c         1179 g         2168 t
ORIGIN      
        1 atctctatta cctatctcac tcttatttaa caaaagtttt ttcatgtcaa atttaatcgg
       61 ctgttttatt tttgtaaatt cgcttctact actaatccaa tccgagatta atttttcaac
      121 atgtcccaga gtaaactctg atttattcgc taaatacttt ttttgttgta ttaaagacat
      181 ctttccccga agctcaaaat tgagcctaca tcgattctct atctcttcaa aaagcatctt
      241 atcttcatca ttcttatatt catcattcat aaaaccaatc cagctcctcg attaagataa
      301 tattttaatc tcatacaaaa actagctctc atcaaaacaa ccattgtttt gatttctcat
      361 gaaatttctt tatacttttc tccgattata aattcctaac cccttgagaa aattacgcat
      421 atatttatct ccgcaaggct taaaatttcg ttggccttct tttctcaaaa atgccccgat
      481 ttcgcctttt gatggataga tttctgcctc agcaaaaacg tctaaaatat catcagtggt
      541 catagacaag gcgattttta atttttttag caacacattg ttgatatatt tgatttcctt
      601 gcttttttgc ggcgtgtcta ctatcggctt atctttttgc ggaccacgcg ccaagagaat
      661 caggccattt aggaatgcgt caaaaacttg gttgttgatg gttttttcat acacattatt
      721 ttctgcatta tctttgttct tatcctgtac tttggtcagt accgcctgag cttcttcttt
      781 agtcagtggt acaccgccta gctcaaaagc tttaatcaaa tccgtatctt tcaaatcaat
      841 cgcataacgc aatcgcaata acctatcatt gttattcatt tgttcctccg aagtatttag
      901 tctttacttt cttacgtact ttttgtcttg ctaagatttt taggatcaat gataaaaacc
      961 gcatcactta tgataaggcg cccccgcatt gatccggaag gcacggtaga tttgctcgac
     1021 aaggatcaga cgcatcaatt gatgtggata cgtcattttt ccaaaagaga tctgagcatt
     1081 gcttcttttc atgacggctt cgctgagtcc taaagagccg ccgataacaa aaacaaggtg
     1141 gctttttcct tggataccta attggtcgat ttgtttagca aaagattcac ttgtaggatt
     1201 tttcccttca attgctaagg catagacgta ttcttgttct ttgattttcg ctaagatccg
     1261 ttccccttct ttttctttta cttgtctcat ttgcgcttcg cttaaattct ccggggcttt
     1321 ttcgtcagga acttcgatca attcgatttt tgcataggca ttcagccgtt tgacatactc
     1381 attgatacct tggatcaggt atttttcttt taattttcct actgagataa ttttgatatt
     1441 catgttttct ctcctttggt cttcatgctc actttgttta ttataatcaa ttttttcgtc
     1501 tatatgttta ttttggctat ttttgttaaa aagatttccc caacagttat gcacaacatc
     1561 cacaggttta tccacagaaa acagtgttat tttaccttat ttcatcacat tcattaggca
     1621 aacatacgtt ttataagaat ttatccactt tatacacagg ctgtggatat gttttatccc
     1681 ttatttttcc aatcctatcc gcgtagtttt acacagtatc tgtggaaaaa gaaaatcaaa
     1741 tattttcatc tgaaaacaat gatttttcct aaagtttttc ataaaaagtt cacagtctca
     1801 aagtagacta aagtcaacaa ataagactat actaacagta gataaatgaa tgtaattgga
     1861 ggaggaacac atggatagaa aaaatgtgac accaaagatg aaaaaaagta aaaataatag
     1921 cctctggcgt aagttaggtc tggggcttgt tggagggatc gttggaggac tactgacagc
     1981 tggcattttt tatgcagcga tgggcactgg caacaatagt tctactgcta caagcggcaa
     2041 tcagaattct gccggtgaaa cagttgtgga aaatgtcaaa gtcaatgtag attctgatat
     2101 caccaaagcg gtagataaag ttcaagacgc tgtcgtttct gttatcaact tacaaagtca
     2161 aaaccaatca agcggttttg gcggactatt cgggcagcaa gaagaaagta gcagctcaga
     2221 tgatagtaat ttggaagctt atagtgaagg aagcggcgtg atctacaaaa aagacgggaa
     2281 tactgcttat gtagtcacga ataatcacgt agtagatggc cagcaaggtc ttgaagtctt
     2341 gatgaaagac ggtacgaagg taaaagctga attagttgga acagatgctt attcagattt
     2401 agcggtgttg aagatcaatt cagacaaagt cgaaacagtt gcctcttttg gtgactcaag
     2461 tgctctgaag gttggtgagc ctgcgattgc gattggttcc ccactaggat ctgaatatgc
     2521 aaattctgtc acttcaggaa tcatttcttc cttgaaccga caagtgacaa gtacgaatga
     2581 atcgaacgaa acagtcaata tcaatgcgat ccaaacagat gcagcaatca accctggaaa
     2641 ttctggtggt cctttagtta atatcgaagg acaagttatc ggtatcaact caagtaagat
     2701 cgcaagtact tctgaatcta cttctaatgt aagcgtagaa ggaatgggat ttgctattcc
     2761 aagtaacgat gtagtgaata tcatcaatca actggaaaaa gacggaaaag tgacacgtcc
     2821 agctctaggt atcacaatgg tcgacttatc tgccgtttct actcagcaac aagaacaaat
     2881 cttgaagatt ccttcatctg taactaacgg tgtcatcgtg acaaatgtcc aaacagctac
     2941 accggctgaa aaagctggat tgaaacaata cgacgtcatt actaagatcg atgacaaaga
     3001 agtttcttct ggtgtagaac ttcaatctgc tttgtataag aaaaaagtgg gagattctgt
     3061 caaaatcact tactatgaag gctcgaaaga aaaaacaact actgttgaat tgacgatcga
     3121 tcaatcggca ttaaaacaaa atcaaaatag tgaaaattaa taaaaaagag gttgtgacaa
     3181 agacttgtca caacctcttt attttggaaa tgcttctccg actacggcat cctcaggcaa
     3241 tactaagatt cctttttttg ctggagcatt tggcaaatgc aattcacgag cggaacagat
     3301 cattccatag ctatctaccc ctcgcaaagt tcctggccag atcatcattc catctggcat
     3361 catagctcct ggttttgcaa cgacaacttt ttgtccagct tcgatattgg gtgcaccgca
     3421 aacgatttgc aatactgtac cattgtctac ttctgtctca gtaatcgata aatggtcaga
     3481 atcaggatgt gggacgcatg tttttacgta cccgacaacg atcttcggtt catgatcagc
     3541 ttcaagctgt tcttcgaatc ctgcttcttt gatcaatcga ttcaatttcg ctaattcctc
     3601 atctgttaag cttacttggc cgttcccttc gattgttaga tgatcggaga tatggaaaaa
     3661 gttccaagca acaacttgac cttgttcatc tttgatccga gcaacattcc atttgcgttc
     3721 aacagtgttt tctgttcctt tatcgtcggc tataataacc atcaaggtgt ctccgacgtg
     3781 tgctttgtta taagcaaaaa tcatgttctt tcctccatta tcctttgctg agatttatct
     3841 taacaaaaaa ttgcattcac tgcatgaaat ttttttcatg ctttatttcc gaagaagcct
     3901 cttctggaac accatttatt cgtaacaagg gactgtgaca agacttttgt cacagtccct
     3961 ttactgtttt agttaattga ttaaaaaggt cgtacaagtt ccacttgctt aattacttgg
     4021 ctgttcttct tcgtcagaaa cgatcaagac atcacaaggt gaatgacgta tcacgtagct
     4081 ggctacactt cctgtcataa agcgttcgac tgcatttaga cctgactgcc caaccatgat
     4141 tagatcgatc tcatattttt ttggcaattt gactgccatg gattcacgaa cagaaccata
     4201 tgcaataatt ccttctatat ttgtgtaatc aacagatttc ccatattctt tacattcttc
     4261 gatcatttcc ttggctgttt cagtttgctg atcgactaga ttttgatcaa taggtgcata
     4321 gcccataaag ttataaaatt gctgatcgat tacgtttgct acatatactt tcccattatt
     4381 acggcgagcc acttctaccg cttttttgaa agcagcaagt gcttgttcac taccgtcgat
     4441 acctactaaa atattcttat attcttgatt catcttgacc cctcctaaca gatgtcagcc
     4501 ttttgttaca ctttctagga acgattcgat ttcctgtttt gttttacgat ctttattcac
     4561 tagacgacct aattcttttc cgtcttggat cacgacaaaa cttgggatac caaaaatgtt
     4621 ccattcagcg gctacatcta tgtacttgtc acggtctact tcaataaatt gccaatcaga
     4681 aaagtcattt tcaatttcag gcatttgcgg tttgataaaa cggcagtctc cgcaccagcc
     4741 ggcagtaaag aaaaatacac tctttccagt ttctacgtag cctgctaatt cttctaaact
     4801 atttgggata atcattcttt gtcctctact ttctgtttat gtatttttca attaaattat
     4861 acacctttct ttcgaactaa caaacattga tcctcttttt ataaaaaaaa tctggcaaaa
     4921 agcaatcttt tgccagattt tatagtttca ctatcgcatt attttccgta tacgatcgta
     4981 ttcactgttg atttatccaa acctttcagt gtttgaatca acatttctcg tgcagcttca
     5041 aaatcgcgaa tactgaacat cgtttgatgc gtatggatat aacgtcccac tacgccaatc
     5101 actgtacttg ggattccttc gttttgtgta tgagcagcac ctgcatctgt accgcctttt
     5161 gaaacaaagt attgataagg gatattgttt gtttcagcga tatccagcaa gtactcgcgc
     5221 aaacgaggaa gcatgatcaa acctggatct tggatacgca tcaatgtacc ttctcctaaa
     5281 tgtccgaatg tgccattttt cgtcactgta tcatctgctg cagagcagtc tactgcaaag
     5341 aacagatctg gcttgaattt attgacagaa gctttagaac cacgcaagcc tacttcttcc
     5401 tggacattcg cgcctgcaat caatgtatga cctaatgttt cattttgcaa cgcttctaaa
     5461 gcttctaata ccattgtaca gccatagcgg ttgtcccatg atttactgat aatattcttt
     5521 ccgttggctg ttttgatcgt ttcagcgtaa ggaacgattg tgtctcctgg aaggacaccg
     5581 taactcattg cctcttctct tgattcaaac ccagcatcga acaaaatatc ggttacttct
     5641 actgattttt gtccgcttgt tcctcgcaat aaatgtgggg gaacggaaga agaaatacat
     5701 ggataattct taccagtaga ggttttaagt gtaaaacgct gagcagaaac aacgtaagga
     5761 ttccagccgc ctagaggtac gactttaaaa agcccgtttt cttggatttg tgtcagcatg
     5821 aatccgactt catccatatg tgctgcgacc attacacggg gtgcatccgc ttcttcatga
     5881 tggcgcagac caaaaatgcc gcccaaacca tctaactgta actcatctac cagcggttcc
     5941 atatgacttt ccatataagc gcgaacatct tgttcaaaac cgcttgttcc ttgtaattct
     6001 gttaactctt tgattcgttg aaatgttttt tcttccatat gctattcgtc taaatttcag
     6061 actaaactcc tctcagactt atattgatct gcggataaaa tatgattttt tctgcagacc
     6121 gatcgtattt attatagctt attttttctg gtagcggtta tttatttcaa agaaaatatg
     6181 agagtgtggt aaaataagca caaggagtaa caagtgagaa gaaggaggta tgtccacatg
     6241 tatgaagaaa gcgaactaag ctattttaaa ggcgggttag cagttggaat cggtctagga
     6301 ttattgagcg gggtagcttc aacgatttgg tatcatcgca aaaaagtatt gtcagcagac
     6361 caagtacttg aaaatatcaa gtcatctttt ctaaaagaag gtgcaatcga aggttcttgg
     6421 atctcttttg aaaaggaacc tattaggaaa ttcgccgttc ggtcgagtgc ttatcgggga
     6481 ggtctttctc gaatagaaga tggcgaaact gtttattatg aatttttagc agatgctcat
     6541 acaggaaccg tattagaaat ttcaagaaaa aaaggtgcaa cagattaata cgtcaaaaaa
     6601 gtgagagaac gaat
//
LOCUS       DANKKG010000133         6453 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000133 DANKKG010000000
VERSION     DANKKG010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.133"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            442..1410
                     /locus_tag="PW004_002376"
     CDS             442..1410
                     /locus_tag="PW004_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2727310.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            1428..2333
                     /locus_tag="PW004_002377"
     CDS             1428..2333
                     /locus_tag="PW004_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2727311.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGSAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            2330..3136
                     /locus_tag="PW004_002378"
     CDS             2330..3136
                     /locus_tag="PW004_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2727312.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            3272..4261
                     /locus_tag="PW004_002379"
     CDS             3272..4261
                     /locus_tag="PW004_002379"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HDL2727313.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMMTSESTMKDAK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(4578..5102)
                     /locus_tag="PW004_002380"
     CDS             complement(4578..5102)
                     /locus_tag="PW004_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HDL2727314.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            5389..>6453
                     /gene="scm"
                     /locus_tag="PW004_002381"
     CDS             5389..>6453
                     /gene="scm"
                     /locus_tag="PW004_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HDL2727315.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT         2035 a         1313 c         1268 g         1837 t
ORIGIN      
        1 cgtttcttct gctttttcgc aacattttct taatgttttc aattttatgt tttcaattat
       61 ctgacaattc ttaattgaca gaccttttta tctcggttat actttagaaa attcattttg
      121 tggaggaact tctatgcgaa ccaacatcaa ccaaatcgca ccaacacaac tgaataactt
      181 ttactttagc tactttagat aggtttgtat tcatcatgat ggatacaaac gttaaacgac
      241 agtttgtatc tatgatggct aaagaacttt gtattgcagc aaaaagccac gtagacaaaa
      301 tgctaagtgg ctgagcagtg aaaatacaaa gtaaaagtat caagcattct actttttcaa
      361 tttattttcc gacttttcaa tccatttgga cattttgtct gaatggattt tttgtttccc
      421 aaaattaagg aggaaaaaga aatgaaaaaa aatataatca tcggattagg cgctgccctg
      481 ctactagctt taggaggttg cgcacaaaaa gacaattccg cagaggcatc agatggacaa
      541 aaaacaattg gcgtattgca acttgtacaa cataactcgt tagatgctgc ctataaagga
      601 ttcaaagaag gattagcaga agaagggtat aaagaaggcg ataatctaac aatcgattat
      661 caaaatgccc aaaacaatca agacaacctg aaaagtatga gtgagaaact cgtcaaagaa
      721 gatcctgatt tgctacttgg aatcgccact ccagcagcac aatctttatt aaatgaaaca
      781 accactatcc cgatgactgt cacagcggtc acagacttgg aacaagccca tttagtcaca
      841 tccaatgaaa aacctggcgg caatgtgact ggcacgacag atatggtgcc aatcgaaaaa
      901 caaatcggct tactgttaaa tatcgtgcca gacgccaaaa caatcggcat catgtataac
      961 aacggtgaag caaactctaa gatccaagct gatttggcag aaaaagaatt gaaaaaagcg
     1021 ggcgtcaaag tcaaagtact gacagcaaat tccacaaatg atgtccaaca agtgacgaaa
     1081 agtctagcta aagatgtaga tggaatctat attcctactg ataacacgtt cgcaagtgcg
     1141 gcaagtgttg tcggtgaagt cgcaaaagaa acaaaaacac ctgtcgttgc tggcgcagtt
     1201 gatcaagtca aagacggcgg tctagcaaca gtaggaatcg actacgaaag cttgggcaaa
     1261 caaacaggga tcatggcagc aaaaatctta gacggcaaga atccaagtga acttcctgtc
     1321 gaaacagcga atgatttgga actgtatgtc aataaagaca tggccaaagc attaggcatc
     1381 aatccagaca gcatcaaaga agctgaataa aacacaggag gattctcatg acaaatttgc
     1441 tctctctttt cttatcatct gcatcgcaag gggtcttatg ggccctactt gcgattggtg
     1501 tatacgtcac tttccgtatc ttggacattg ccgacttgac tgctgaagga agctttcctt
     1561 taggtgcagg gatcgcggca gtaagtatta caaatggcta tcatccattg acagcttgtc
     1621 ttctaggctt tttagggagt gcggcagcag ggcttgtttc cggactgctt cacacaaaat
     1681 tgaaaattcc cgcccttctt gcagggatca tcaccatgac tggtttatat tctgtcacat
     1741 cccgtgtgat gggtgcgccg aacatctctt tattgggtca gccaacgatc tttcattggg
     1801 tcgagtcatt cggcataaca aaagacaacg ctgtactgat cgtcggtctg ctttttgctt
     1861 tgatcgttgt atgtttactt gttttgttct tacgtacaga aacaggactt gctattcgtg
     1921 caactggcga taatctggca atgagcgaag caaacgggat caacacagat aatatgaaga
     1981 tcatcggtta tatgatttct aatggatgca ttgctctttc tggctccttg cttgcgcaaa
     2041 acaacggctt tgctgattta aattctggta ttggaacgat cgtaattgga cttgcttcta
     2101 ttatcatcgc agaagttttg ttacgtaacc agccactatt tctccgtctc ttgactgtcg
     2161 ttttaggtgc ggtcatttat cgttttattt tagcgttagt cttcgaactg aatgttgaac
     2221 caagtgattc gaaacttgct tctgctttcg tgctagtgat ctgtctttct ttcccgcaga
     2281 tccagcaaaa attccattta cctcaaaaaa agaaaggcgg tagtgtcaaa tgaatgacgt
     2341 cttaacattg gaaaatctcc atcatacctt tgaaaaagga acgatcaacg aaaatcatgt
     2401 cctgaaaggc attaatcttt ccttagatcc cggcgacttc gtgacgatca tcggtggaaa
     2461 tggggctggt aaatctactt tgctgaacag catcgccggt acattttctg tcgatcaggg
     2521 tcaaatttta ttgaatggaa aaaatatcac gaaaaaatct gttgtcgaac gttcgaaatc
     2581 catcagtcgc gtattccaag atcctaaact agggacagcc gttcgtttaa cggtagaaga
     2641 aaatcttgct cttgctatga aacgaggaaa aaaacgcggc ttcttccgtg gtgtcaaacc
     2701 acaagaccga acatttttca aagaccagct ggttcggcta aatttggggt tggaaaatcg
     2761 tttatcctca gaaatcggtt tgctgtctgg aggacaacgt caagctatta cgttattgat
     2821 ggccacatta cagcgtccag aactgatctt attagatgaa cacactgcag cgcttgaccc
     2881 tgctacttcc aaaacggtga tggaattgac agaaaagctg atcactgagc agaatctcac
     2941 tgcttttatg gtcactcatg acatggaaga cgcgatccgc tatgggagtc gactgatcat
     3001 gctgcatcaa ggaaggatcg tcgtagatat ccagcaagag gaaaaacagc agctcacagt
     3061 gccccaatta ttagcgttgt tcaaacaaaa cagcggcacg gcattaaaag acgatcaagt
     3121 acttttatct tcataatcag aaagaggatg ggacaaaaaa tgaacgccca ccctcttctt
     3181 ttttctcttt ccttctttcg tttttgcttt atgtatcaac cgttgtttct tcgaaaacta
     3241 ttttgttctc tccttcttcc tgatagaatg gatgggaaag gagcttgatg ccatgacaac
     3301 gaaagacaag gtacttcaat tattgaaaaa gacaccagat tttctttccg gagaaaagtt
     3361 ggcacagcaa ctggaagttt cccggacaag tatttggaaa gccatcaaag aattggaaaa
     3421 ggcaggctat cgttttgagc acgggcctac agggtatcgt tacctgccct ctgacgtact
     3481 agatgcaaaa gaaatccagc aagagctacg gtcgtttatt cctgatttga aagtacaaac
     3541 gatgatgact tctgaatcta cgatgaaaga tgccaaactg gcagcagctc aaggagattc
     3601 tgtttcgaag ttatttgttg ctgatacaca agaacaagct cgtggacgtt ttggacggcc
     3661 ttttttcgct gaacctggac gaggaatcta catgagtctc ttgctccatc cagacaaacg
     3721 gtttgatgaa ttgcctcaat atacgatcat cgcagccagt gcctgtgtga aagccattga
     3781 atctgtaacc ggtcgttctg cttcgatcaa atgggtcaat gatatctaca tggatggaaa
     3841 aaaagtctgt gggattttat ctgaagcagt cagtgatatg gaaagcggca ggatcagtca
     3901 tatcatcatt gggatcgggc ttaatttttc tgttcctcaa aatcattttc cagctgatct
     3961 ccaagaaaaa gccatctctg tctttcccga cggacaagct agtgtcacaa ggaatcagct
     4021 aatcagcaaa atctggcaag aattgttcac ttatctttct cacccagaaa aagcgttgga
     4081 cttctataaa gaaaaatcat tcgtattggg aaaaaccgtg tcgttcaatc aaaaaggaac
     4141 gacttatgtc ggtcttgcaa aagaaatcgg tgatcatggc gaactgatcg tccaaacaaa
     4201 tgaacagaag atgagtttgt cttctggcga aatcagtctc acatctatcc gtacgcaata
     4261 attatcagat ttcacaaaag gacacctgat ctcatacagt gtcctttttt ctgaattctc
     4321 ttcaaaagac tctccttatt ctatcagctg acagtttctc tatcctctag aaaaaggtat
     4381 tctaaaaata tctataaagc atggtacact ttctttaaat gaatcaatgc aaaaaacaag
     4441 agagtcatca gtttacacga tcaaaagaga tagcaggccg cttccttttt caatcttata
     4501 gatagaccat gggtaacgat aaaaacaaaa aagaacttgc gacttatttt gccgtaagtt
     4561 cttttttagc tagtgcctta atctcttaat cccaacaaac gtctcgcata gatcacctga
     4621 ccagagtgca tagcagcatc atcaataata gaaacgagac gcattcctcg tgtgactggt
     4681 ggtgtccagt tctcgtcaat tacttcatct agctcggaag gttccagcct tcttaagtaa
     4741 gaaatagcaa aatcagcagc tcctcgcaag tatcctagca gatcttctat ttgattagct
     4801 tggatctgct ttgcttgttc cagactatgc ttccaatctt gttcgttatc tgctatcgtc
     4861 atcggaaacc ggctttccca gtcttgacta tgccaaattg gagaagttcc tgctaaatca
     4921 gcaatctgga aatctaattc tcgagcggta tgccaagcca gccaagtcat tgatttgatc
     4981 tgcggggctc tatcttccac tggaaaacga ttggcctcct caatagagac gccttcaaac
     5041 aaacgttcaa accgatcgta tgcccgatcc acactctcgc atagcatatc taaataatcc
     5101 atttattttc ctccttttta tttatcctac ttactttttc ctaattctag gatacctact
     5161 tatatgttac tacacaccct tattttttac actttaacag tcaccatcat caatagtttg
     5221 gagattgaaa gaaaaagata gtgtcgaact atcaattgaa caagaagcaa caatatgaac
     5281 gtttcattaa tattctttaa ataaacaatg atttttcaaa cgaacagttt aagactagga
     5341 gaaaaagagt ataataggaa aatgtttgaa aggaaaggaa ggggaaaaat gaaaaaaagg
     5401 gcaagtttac tagtcctagt tgctttgttg ttcaatatct tttcgtatgg tgtggccgtt
     5461 tcagcggaaa catacattcc taaagaccct gggaatattt ataatgatct aactggtaac
     5521 tatggcttgt taggaattgc tagtcaattc cacgtatttg ctaaaaataa gacaactgtg
     5581 aatgcacata cagacggaaa tatagcaaca catgaacttg atgcaaaaaa taattttggt
     5641 acagagcttc atgaaggttt attgaaccaa gaaataaatt atatacaaaa aactgattct
     5701 ttcgctaatt cttcaggcat acctacaggt agcacaagaa tgaataaatt tgtcgttggc
     5761 agtaacgtgg atgtagcact tcaagatggt catcctacag tcaatggatc caggcttgat
     5821 cacttaactc cagatgattt ttataaagat cgtgctgggc atgaatatat tgatttcgag
     5881 caggaatttg ctaaattgaa tgtagcagca aatgatcttg caacgatcac tccagctaaa
     5941 acatatactg ctgcagattt tccagacatg aacaatcgga caatcgattt aactgattta
     6001 tcagataccg ggtttttatt ggtaaatatc gatgcagaag tgttaacgat gaatacgcca
     6061 cttcaaatca ttaatccaaa tgatcaagta gttgtattta atgtcatcaa cagttcttct
     6121 gcattgaacg tacagtcacc aatcaaatac aatgatcgtt caaatcatga aacagaagat
     6181 ttttcggatg cgaatatctc ttggaatttt ggaaatgaga tgactgattt gacaatcagt
     6241 gctccttttc aaggaacaat ccttgcgcct aatgctacta tccgcattac tcaaaaccaa
     6301 gacggaacga ttataggaac aaatgtgatt ttagatgcag ctaccaatcg ttgggatcct
     6361 aacgaaatat ttatcagcga caatggtaca gatacgacag acacaagcga cagtacagat
     6421 acatctacca caagtgatag ctcagattca tca
//
LOCUS       DANKKG010000134         6343 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000134 DANKKG010000000
VERSION     DANKKG010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.134"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(93..290)
                     /locus_tag="PW004_002382"
     CDS             complement(93..290)
                     /locus_tag="PW004_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727316.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(385..2145)
                     /gene="efrB"
                     /locus_tag="PW004_002383"
     CDS             complement(385..2145)
                     /gene="efrB"
                     /locus_tag="PW004_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HDL2727317.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(2142..3872)
                     /gene="efrA"
                     /locus_tag="PW004_002384"
     CDS             complement(2142..3872)
                     /gene="efrA"
                     /locus_tag="PW004_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HDL2727318.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            4265..4477
                     /locus_tag="PW004_002385"
     CDS             4265..4477
                     /locus_tag="PW004_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HDL2727319.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            4479..6158
                     /locus_tag="PW004_002386"
     CDS             4479..6158
                     /locus_tag="PW004_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDL2727320.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
BASE COUNT         1968 a         1328 c         1131 g         1916 t
ORIGIN      
        1 catatattta tttcataaga aaaaaaacag gtaaaacctg ctcaggccag atgtctattt
       61 acagtatctg gtaactgacg ttttacctgt ttttattgta catctatatt ttcttctatt
      121 acttttttta aaattaattc gtacacttct atttgtgctg ccaagcgttc gatttgaaac
      181 tcattttcaa aaaaatcagc tgaatcaaaa ggcttcgtct gacagacgtg cgttaaacga
      241 tcaacttctc ttttggcttc agtaattttt tcatttaaaa actgttccat ctccgtgtcc
      301 tcacttttct tgataacttt actttaccat tatcaagaat agagaacaaa aaataagttt
      361 taaactgttt tattcttata tgacttattc tgcttcctca gcaaattggc tgttatacag
      421 tttttcataa aatccacctt gtgccaataa actctcatgc gttccttttt caatgatctg
      481 accttgatcc atcactagaa tcagatctgc atcacggatt gtcgataaac ggtgagcgat
      541 gacgaaactt gtccgtccct ccatcacgcg atccattgct ttttgaatca acgcttctaa
      601 acgggtgtct actgaacttg ttgcttcatc taaaatcaag attttaggat cggagaccac
      661 tgctcgggca atcgtcagca actgtttttg tcctaaagac acattgtctc cctcagaatt
      721 gatggccata tcatatccat ctggcatcgt tcggataaaa tgatctacgt ttgctgtttt
      781 cgcagcatca actacttcat aatctgtcgc atcaagcttt ccgaagcgga tattatctgc
      841 aatcgttcct tgataaagcc atgcatcttg caagaccata ccaaatagcg aacgcacatc
      901 actgcgactc attgatttgg tgtcgattcc atcaatcctt attgctcctt tgtctacgtc
      961 ataaaaacgc atcaatagat tgatcaatgt cgtcttgccg gcaccggtcg gaccgaccac
     1021 agctacggtt tgaccagctt tcacttcaaa attcaaatct tggattaatg gtttcttcgg
     1081 atcatatgca aaatcgacat gatcgaatgt cacatctcct aaaatcgttt ctggcagtga
     1141 agcatctact ttgttttctt tttcttctgg ctcatctaaa atctcgaata cacgtttcgt
     1201 tgccgctgat gcactttgca aaactgaaga caattgtgtc acgttcccca ttggctgact
     1261 gatttgccaa atatattgga tgaatgcttg tagttgaccg acaacgatcg ccccagtaat
     1321 aacataaaag cttcctagaa cagccatacc aatataagta gcataggcag ttaattgaac
     1381 tagaggcatc atcaagccag aaatgaatgc tgcacgaaac ccaaagtatt tcaagctgtg
     1441 attaactttt ttgaatcctt ccaatgtttc tttttcgcgt ccatatactt tcaatacact
     1501 aaaacctgtc atattttctt ggacgtagcc gtttagctca ccaagagaat tctgcatgct
     1561 ttgaaaatat ttctgcgaag cattgatcac tcgttttgag atccagatcg atgttggaat
     1621 catgatcata gagatcagcg ccatcatcgg ctggatataa aacatcattg ctgcagccaa
     1681 cgtaattccc aatatcgcat ttacgatacc aataaaagct tgttgtaaag caccgctgac
     1741 tgcatctaca tcattcgtca cacgagacaa aatatttcct tgctggtttt tatcgaaata
     1801 tgaaaccgga agacgattga ttttttcttc tatgtctctt ctcaaatcat gcatggcact
     1861 ttgaacgaca tttgtcatca aaacaccaga aagtaattgc gaaatacaat accctattcc
     1921 aacaactgtg atccaaatga gacatttgaa tatataagaa aaattgattg attcgccatt
     1981 ggcaatatta cggctgattt ctgtagtagg aagtcctgta acatatggca aagccacgtt
     2041 gaaagccact gtcagaactg taaataggat cactaaaaga aaagtctcac gatacggtct
     2101 gatatatcga gctaaacgtt ttaaggaaga aaaggttttt ttcatgctaa ttcctccttt
     2161 gataattgag aagcggcaat atcataataa atcgggcatt tttccaacaa ctcccgatgc
     2221 gtccccatac cgacgacttc tccttcattt aatacgatga tcttatctgc atgcatgatc
     2281 gttcccacac gttgtgctac gatcaataca gtagattctg tcgtttcttt tttcagacga
     2341 gcacgtaact tcgcatccgt ttgataatct aatgcagaaa agctgtcatc aaaaatatag
     2401 atttccggac ggcgtatgat ggctctggct attgccaatc gttgtttttg tccacctgaa
     2461 aaattggaac caccttctga taaatgttca ttaaatccat ctggttttcg agaaatgaat
     2521 tctgaagctt gagcaatctc agctgcacgt tccatttctt cttgcgtagc atcttctttc
     2581 ccgtatcgca aattatctgc aatcgttcca gtaaatagca gcgctttttg cggaataaac
     2641 ccaatcttat ctcgcaactt gcttagccga taatctctca cgtctactcc atcgatcaag
     2701 atctttcctt cactcacatc ataaaagcga gggatcaatt ggatcaacgt agattttccg
     2761 ctaccggtac ttccaataaa agcaacggtc tctcctggag aggctgtaaa actgacattt
     2821 cgtatcactg gctcttgcga atggcctgga taagcaaatg ttacattttg aaattcgata
     2881 taccctttag tttttgtatc agatatacca tttggatctt ctacaatcga aggtttggca
     2941 tctagggctt cttggatacg tccagctgaa acagctgctc ttggatacat catgaacaca
     3001 ctagcaaata acatgaatga gaacaaggca tggaatatat actcgataaa cgcaatcaaa
     3061 tctcctacac gaagctcgcc tgcatcgatt tgcactgccc cgctccagat gatcaatacc
     3121 attacgatat taaataagaa ataaaaaccc ggttgtgcaa cagccatcaa acggaataag
     3181 ctttttgaac tagaagcata attctcattg actacagcaa aacgtttttc ttcaaacttt
     3241 tcattgacga atgcacgaat cacacgcaat cctgaaagat tttcccgcaa gatactgttg
     3301 attttatcta agtttttttg ttgcttttta gataacggtt cagacacctt agcaatcgct
     3361 acgactgcca ataatagtaa aggtaatgca cctactacat acaaactgag tgtcacactt
     3421 gtacgcatga ccatgtacaa actgacaatg aacatcattg gtgccataaa accgatacgt
     3481 aaaatatttt gcaggaacaa catgatctga taagcatcgt ttgttgtacg agtgatcaat
     3541 gaagatacgc ctagattttc gtattcctga tgcgaataac tttggatctt ttcgaacaaa
     3601 tcatctcgaa tatctgctac gatattagtc gtaatcctcg aaccaaaata acctaacaag
     3661 atattcaaaa taataccgat tacagtaata acgatcataa atatcccttg ttgcttcaca
     3721 taatcaaaat catcattgcg gattcctaca tcgatcatac gtgctaaaat cgtaggaagt
     3781 cctaattcaa tcaaaataaa accaaatacg catatgaaat tgaaaaacaa gaatttctta
     3841 taacgcatgg tataatgcca cattagtttc actttctcat ctccttttaa tcttttgtta
     3901 cattttcatt actaaaaatg atagcacaac gagcctatta atagaacact tctctcataa
     3961 aaaagtcatt atgattatag cgggatgata aacattgatt ttttctttca atctagttga
     4021 tttctatact tatttgctgt tttatgctag aataacaatt aattgttgta tattttttat
     4081 acagaaggta cggttgaaaa aatatccgaa cagataagca aaaacgaggc ttgtctcttt
     4141 gactcgaatc cagtattcta gtcaaagcat atgaaaaacc aggtggcttg tcccctcgtt
     4201 ttgtgttgtc taaaaaaatc aactagctaa atcactgctt tacgcagata aaggagacaa
     4261 aacaatgatc tataaagttt attatcaaga aacaaaagta cgcaacccta aacgggaaga
     4321 aacaaaatct ctttacattg aggcaaaaag cgatgtagac gctcgccaac gtgtggaaga
     4381 aaatacccca tacaatattg aattcgttca acttctagaa ggaagtcatc tagagtacga
     4441 aaaagaaaat gctgatttct cattagtgga gttttaatat gaaactttcc atcaaaaata
     4501 acgagacagg cgtctttgct attggtggat taggtgaaat cgggaaaaat acgtatgggg
     4561 tacaattcca agatgaaatc attttgattg atgccgggat caaattcccg gaagacgatt
     4621 tacttggaat tgactatgtc attcccgact acagctacat catacaaaac ttacacaaag
     4681 taaaagcctt ggtgattact cacgggcacg aagaccacat cggcggaata ccctacttgc
     4741 tgaaacaagc aaatattcct atctatgctg gtcctctcgc attagcgttg atcacaaaca
     4801 aattagatga gcatggcttg cttcgagatg cagagctcca tgagatcaat gaagatacga
     4861 tcatccgttt tagaaagact tctgtcagct ttttcagaac gacccatagt atcccagatg
     4921 cattagggat cgtggtgaaa acaccttctg gaaatatcgt agcgactgga gacttcaaat
     4981 tcgatttcac tcctgttggc gaaccagcaa acttgcataa aatggctcgt atcggggaag
     5041 aaggagtctt gtgcctttta tctgatagta caaatgctga gattccaaca tttactaaat
     5101 cagaaaaaac gattgggcat tctattttga agatcttttc aaaaatcgaa ggacgtatca
     5161 tttttgccag ctttgcttct aacatcttcc gtttgcagca agcagcagat gccgctgtgg
     5221 caactggtcg taaaatcgct gttttcggac gaagcatgga aaatgccatc gtcaacggtc
     5281 aacgtttagg atatatcaaa gttccagacg gcacatttgt agatgcacac gaaatcaatc
     5341 gcttgcctgc caatgaaacg atgatcctat gtacaggttc tcaaggagaa gcgatggccg
     5401 cgttgagccg catcgcgaat ggtactcacc gtcaaatcca aatccagcca gacgatacag
     5461 tgatcttttc aagttcacct attcctggta atacaacaag tgtcaatcat ttgatcaatt
     5521 tgctgtctga agctggtgca gaagtgattc acggaaaaat caataacatc catacttccg
     5581 gtcacggtgg acaagaagaa caaaaattaa tgcttcgttt gatgaagcca aaatatttta
     5641 ttcctgtcca cggagaattc cgcatgttga aagtacatgc ttctctagca caagatgtag
     5701 gtgttcctgc agaaaactgc ttcatcatgg aaaatggcga tgttttagcg ttaactagcg
     5761 attctgcgcg tcctgctggg cattttgtgg ctggagatgt gtatatcgat ggtagcggga
     5821 tcggtgatat tggtaatgtc gttttacgtg accgtcacct gctttcagaa gaaggtcttg
     5881 tattagctgt cgcgacagtg gatatcaaga aaaaagaaat cttggctggt ccagatattc
     5941 tctctcgtgg atttgtctac atgcgagaat caggagaaat gatcaatgaa gctcagcgaa
     6001 tcttattcca cgctttaaga gaatgtttga atggacctga ctgttcagaa tataaattga
     6061 aagaagcgat gactggcgcg cttcagcctt tcttattcga acgtacagaa agacatccaa
     6121 tgattttgcc aatggtaatg acaccagatg aagaataaat aagaggttat gaaatcagca
     6181 ccttgtcctg aatataagag aaaacaaatg aaaaacatga gaagccattt ttgtttgttc
     6241 ttattccttg atacaaggtg gctctttcat agcctcttaa atttatttac tttttcatac
     6301 aaaaaaatac ctgccacaag agaaattgtc tcttgtggca ggt
//
LOCUS       DANKKG010000135         6319 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000135 DANKKG010000000
VERSION     DANKKG010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6319
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.135"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(99..1229)
                     /locus_tag="PW004_002387"
     CDS             complement(99..1229)
                     /locus_tag="PW004_002387"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HDL2727321.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="PW004_002388"
     CDS             complement(1878..3080)
                     /locus_tag="PW004_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2727322.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="PW004_002389"
     CDS             complement(3140..3343)
                     /locus_tag="PW004_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HDL2727323.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="PW004_002390"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="PW004_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4880..5692
                     /locus_tag="PW004_002391"
     CDS             4880..5692
                     /locus_tag="PW004_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2727324.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1877 a         1299 c          996 g         2147 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaat
//
LOCUS       DANKKG010000136         6274 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000136 DANKKG010000000
VERSION     DANKKG010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.136"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            14..292
                     /locus_tag="PW004_002392"
     CDS             14..292
                     /locus_tag="PW004_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727325.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            311..1519
                     /locus_tag="PW004_002393"
     CDS             311..1519
                     /locus_tag="PW004_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2727326.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(1667..2032)
                     /locus_tag="PW004_002394"
     CDS             complement(1667..2032)
                     /locus_tag="PW004_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HDL2727327.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(2179..2952)
                     /locus_tag="PW004_002395"
     CDS             complement(2179..2952)
                     /locus_tag="PW004_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL2727328.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(3126..3986)
                     /locus_tag="PW004_002396"
     CDS             complement(3126..3986)
                     /locus_tag="PW004_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HDL2727329.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(3986..5404)
                     /locus_tag="PW004_002397"
     CDS             complement(3986..5404)
                     /locus_tag="PW004_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2727330.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(5461..5910)
                     /locus_tag="PW004_002398"
     CDS             complement(5461..5910)
                     /locus_tag="PW004_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2727331.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
BASE COUNT         1813 a         1312 c         1080 g         2069 t
ORIGIN      
        1 agagggaaaa aacatgaatt actggatgaa cacaatcatt aatcgattag aaacagcata
       61 tcaaacaaga tttgatatga aagcttcttt agtcttttta aatgatgcgt atcaaaactc
      121 gatcgaattg atcaaagcag tggatgagaa tccaacaaac gaatgcgaag aatttttaaa
      181 tctgtttatg tctacaagag atttatttat tcgccaactt gttgatcgct atccatccaa
      241 ttatcacgat gtagaagttc aaatacaaaa attaaaagca tattcagctt aaggggaatg
      301 tataacatac atggaaaaga aattatttaa taaatatttt attggaatca cattgatcaa
      361 ttttattgtc tacctgattt attatctgtt gatggtcatc atggcagtca ttgcgcaaga
      421 caccttacac gccagcttga gccaagcagg ttttgcttct gggatctata tcatcggaac
      481 attgctggca cgcttattca tggggaaaca gttagaacta tttggtcgga agtttacctt
      541 aagaggcggc gcgatcttct atctcctttc aacattagca tatctttata cgccaactat
      601 cggggcattg tatttagttc gattcttgaa tggattcggc tacggtacag tttctacagc
      661 tacaaatgct atcgttactg catatatccc tgcatctaaa aaaggggaag gcatcaatta
      721 ttatggatta agtactagtt tagctgccgc tgttgggcca tttttaggaa tgattttgct
      781 gaatatgact gattttcgtt tcattatctg gttttcaatt gtactggtct tctttgttac
      841 gattgcctgc cttgtctttc ctgtaaaaaa tattccatta tctgaagaac aaaaaatatc
      901 tcttcgctca tggaatatca atagttttat tgaaaagaaa gcacttttca ttacaggaat
      961 tgcctttttg atgggtcttt cttattcaag tgttttatca tttttatctt cttatacaaa
     1021 agtaatccac ttagttgctg ctgggtcatt tttcttcgtc gtatatgctt tggtcatcac
     1081 atttacacgt ccattgactg gaaggatttt cgatgtcaaa ggagaacaat acgtgatgta
     1141 tcctagctat ttattcttaa cagcaggatt atttttactg agtgtaacga cgaatagtgt
     1201 gacattgctt atctctggag cattagtcgg cctaggttat ggtacgttta tgtcaaatgg
     1261 acaggctgtt tgtttaaaaa tcgtccgcga acatcgaatc agtattgctt tatctaccta
     1321 ctttattggt ttagacctag gcttaggcgt tggtccttat ttgatgggga tgctaaaatc
     1381 tcttacttca tttagaggat tgtatgtgat tgccggagtc attccattga tatgtacagc
     1441 actctatctt gttaatggcc gaaaacatag tcatgaagca gagggcgaat tgattgaatc
     1501 attgaaaaat gaaatttgat cttgatgaat agataaaaaa aaccgttcag atctgaaaat
     1561 aatctgagcg gttttttgag taaaaatagt ttatgattat tcttaattaa acatctattt
     1621 atttttaaag ctttcattta tcgataagaa tagtttaact gttgatttat tccattttcc
     1681 gttcatctgg tacgatgcct tgacttagca accacttgtg gaagaaccat tctgccacac
     1741 cgatcaatag agcaggaatc aatactttca tccaaatatc gactgtatca ttcataaaat
     1801 atagatagag ccacaagata actgcagctg aaccagcatc tgcgatcgtc gctgcgatgt
     1861 tccctacttt acgtaagatc aacagatccc ctagcacata gatcatgact acaataatta
     1921 ccccaacaat cgttgaatcc ataaagctaa caccgtataa acctgtcagc acgacccaca
     1981 gaatcgccca gatcatgatt gccttcacta ccaacgcctt cacatgtttc attttctgca
     2041 ctcctttctc ctggataaaa aagtaagatt atctaacaag ataacctttt cattattata
     2101 ccatcggctg agagaagcga gcaaagataa ggtcctatct aaaaatgagg aagccactta
     2161 gaacattcac gtaataattt atccgtacag acattcgata tggtgttcct ttactttcgc
     2221 atactgttct tttgtcagtt ctttacttgt caccagataa tcgatttcat gaaaagcaat
     2281 ggcttgatgc atgaatttat gtgcaatttt ttgctgtgta actaaaacgg caagccggtc
     2341 tgattgtttt ctaaaagctt ttttgaccat tgcctctcct tcatgagttt ctgttactcc
     2401 ttggctgaaa tctagcccgc gacaactcat tatcgctaaa tccgcataat atgacaatgc
     2461 atcgttatag gcttttgaac cgttgattgt tgctcgtttc ggaacaattg cccctcccaa
     2521 tacagatact tttgccgttg tatattcact caacatagca gctgtgacga tgccattcgt
     2581 aatgatctgc aaaccatcaa atcttgctag gtttctgact aagtgtaaag aggttgtact
     2641 ggaatccaag aataagacca tatcatctcg aatcaaagaa gaagctgtat ctgcaatctt
     2701 acgtttttct gtttcttctt ctaatttggt gatcttcatc aggtaaggca cgtcaatttt
     2761 tcgattgtca ataagcatgg cgccaccgcg aactctacgg atcaatccac ttttttccag
     2821 aaaaacaagg tcacgtttaa tcgtggcttc gctataattt actaactcga tcaaatcggt
     2881 gactcttgca aattggttgt tttttagata attcacaatc tcctgctgtc tattctccac
     2941 ttcattcaac ataaaatcac cccatttact ttatatagta acaggaaaaa agaggttgtg
     3001 acagaagtga ttggcttcga gaaataagaa gaaatttttc gaaaaaacta cttctgaaca
     3061 tcgtgtgtgg ttaagagggt atgacaagac tattgtcata ccctcttgac cacacatttt
     3121 tatttttagt ataaactagc cttgcctact gtgttcaaaa tttccatttt atgtttgacc
     3181 acttcttgta cctttttatt agctgctgga taaattgcat tcggctcgta catcgatgga
     3241 ttttcactca atatttctct caaagcattg aaaaagacgc ttttaagatc tgagctcaaa
     3301 ttcactttgg caactccgta ttttactgct tcgctgactt ctgcgtccgg attacctgaa
     3361 ccgccatgta aaacaaatgg aagatcgatt tgttggttca attctttcag taagtcgata
     3421 tttaattttg gagttctgtt tttcggatac aagccatgcg ctgttccaat tgctacagca
     3481 agtgtatcga ttcctgtttc tttcacaaaa atcttcgctt gatcaggatc tgtataaatg
     3541 attgtttcag cacctccttc agcagaacca ttattcccaa tcgttccaag ctcagcctcc
     3601 actgagacat ttaccgcatg agctaacgca accacttttt tcgtaatctg tacattgtcg
     3661 tcgaatgatt tcaaagacgc atcgatcatc acggaagaat agccgtttcg aatcgcccgt
     3721 aagacatctt ctatcgtacc gccatgatcc aagtggataa cgatcggcag tttacttgta
     3781 taagcatagg ctcttactgc cgcaacaaac tcatcactta aataggcgat ttcatcagga
     3841 tgaatttcta agattactgg tgcttctaat ctctctgctt cttcgatgat tgctttcatc
     3901 atatcatagc tgcaaatatt gaaggctggt actgcaaatt gattctcttt agccactttc
     3961 aataactctt tcattgtata taacattacg cttccacctc atttgttaga ttttcatttt
     4021 tcgtccactt gtcttgtttt ttctctttca ctgttttttt caatagtcca agcataaagg
     4081 ctgtagtgac tgtacctgat gcaattgcca agacatacat aggtaaattg ttcataacat
     4141 ttggaattag catcacccaa atccctccat gaggtgccat tgaagtaact cctactgcca
     4201 tggaaatccc tgcagcgact gctgaacctg tcatcaaact aggtatcacg cggatcgggt
     4261 cagataccgc aaacggtata gcaccttctg taatatacga agcgcctaaa atccagcatg
     4321 attgacctga agtccgttct tctttggtaa atttatgttt gaataataaa gtggctaaag
     4381 caatacctag gggaggtgtc attcctgcag ccatacaagc agcaatcggt gcatttactc
     4441 cagctgacat caaaccgatc gagaaagtcg aagtcgattt attgatcggc ccacccatat
     4501 cagctgccat catgatcccg ataagtaatc cgaacaatag cccggatgta tttcccaagc
     4561 tattcagcca atttgtcaaa gcatccaaca cccatgtgat cggttgttca atcacaaata
     4621 ccatcaaaag cccaataatt aagatcgata aaaaggggac gacaatcagc tgataaatag
     4681 cagaaaattc tttttttacg ctaattttat ttgccagaaa atcacagata taccctgcta
     4741 gcacgccacc aatcattccg ccaagaaaac ctgaaccacc tgtagaggcc agtaatccgg
     4801 caacaattcc tggggcaaaa cctgcttttc cagcaatcga tgtagcaatc cccgcgccta
     4861 acgcaggaac catcatggca aacgctgtat ctcctccgat ccttgaaaat gcagctgcta
     4921 acacattata gtctgctgag ttgggatcag ccgctgtaat cccaaaggca aaactcagtg
     4981 cgatgatgat tccgcctgcg atcacaaatg gaagcatgta attcacacca ttcatgaaat
     5041 gattatagat tgtcggcttt ttctcatttg cagctggttc tttttcatta gacacaacga
     5101 atacgccaat atctctcaat gcttcgtcta ttactacttg cggttctttg attgctcggc
     5161 ttgtagagac acgctttact tttttcccgg caaatcggtc caattcgatt tctttatcta
     5221 ctgcaagaat gattgcatct gcctctttga tttcttgttc agataactga ttttctgttt
     5281 ttacaccttg tgtttcgaat tttgcttgat ggtttagatt tcttgctgtt acttctaatt
     5341 tttctgccga catataagta tgagcaattc cagtcgggca agcagttaca ccaacgattt
     5401 tcatgattat tttcctcctt tttcttcact atcgatactc ggacaaagac tctactattt
     5461 tcattcttga ctaatctgtc tgatgatatc tgctggcgta gtggcttctt ttattccctt
     5521 tctaaaatct gcattcatca atcgtctgga gagtgtgctg agaatctcta gatgcatatc
     5581 tgctgatttt tcaggaacta cgatggcaaa aatatattca attggcattt catctatccc
     5641 gctatagtcc gttaatttgg ctgtatgaaa aaaacacagt gctggttttg tcacatattt
     5701 gctttttgca tggggaatcc caaatccatc ttctattcct gtggagatct cctcctctcg
     5761 tttgaatagt gccttaacga ctttttgaac atttcttacc ttatttgttt ttttcaacac
     5821 atcccctatt cgctcaaata atgatccttt tgaatctatc gatggatcga aaacaatcag
     5881 ttcttctgcg atcaattgat caaacctcat aaaaaacctc tcccccacaa aaaatgttaa
     5941 cgttttcttg gaactgaggt cagtatagca tcgctagctg taccgttcaa tattatgaat
     6001 gaacagagga aaaagaccca tcatccgcgt gtataagtca ttaaaatgaa cgatctagct
     6061 cacaaacaat cgcaagtcta taacaaaaca aaaagccttc cttgaaagga aagcttagct
     6121 tctaatttta ggtaaatggt tttttacttc gaaataaccc ctcttcattt cgttaatata
     6181 aggtgttttt atcaagtgga taattaaaac tatatactag tttttcattg ttttgtgcat
     6241 tatgatccta cgactttttc agcatcaaac gaag
//
LOCUS       DANKKG010000137         6095 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000137 DANKKG010000000
VERSION     DANKKG010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6095
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.137"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(75..605)
                     /locus_tag="PW004_002399"
     CDS             complement(75..605)
                     /locus_tag="PW004_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDL2727332.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
     gene            complement(787..1443)
                     /locus_tag="PW004_002400"
     CDS             complement(787..1443)
                     /locus_tag="PW004_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HDL2727333.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            complement(1570..2043)
                     /gene="dfrF"
                     /locus_tag="PW004_002401"
     CDS             complement(1570..2043)
                     /gene="dfrF"
                     /locus_tag="PW004_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HDL2727334.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            complement(2438..3829)
                     /gene="amt"
                     /locus_tag="PW004_002402"
     CDS             complement(2438..3829)
                     /gene="amt"
                     /locus_tag="PW004_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HDL2727335.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGFLAMTISMVVSLYEYPTFATSGFSLVFFLLLGGLLWFIPVALCAAEMATV
                     KGWEKGVSQTKRY"
     gene            complement(4170..4412)
                     /locus_tag="PW004_002403"
     CDS             complement(4170..4412)
                     /locus_tag="PW004_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDL2727336.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(4444..5001)
                     /locus_tag="PW004_002404"
     CDS             complement(4444..5001)
                     /locus_tag="PW004_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDL2727337.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(5014..5202)
                     /locus_tag="PW004_002405"
     CDS             complement(5014..5202)
                     /locus_tag="PW004_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HDL2727338.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(5215..5781)
                     /gene="amaP"
                     /locus_tag="PW004_002406"
     CDS             complement(5215..5781)
                     /gene="amaP"
                     /locus_tag="PW004_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HDL2727339.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT         1843 a         1278 c          892 g         2082 t
ORIGIN      
        1 aaaatcttaa ggattttccc gccaacgtgg tcggatcgct ccgtcggttt aacagctttt
       61 ctgtttctct ctagttatct acgcatataa atcaaatcgg ttattccgtt ataagtctgt
      121 gttttacaaa gccttaactt tatttccctg tctatgttgc ccaaaagacg aaccccattt
      181 cctaacagag taggaataac agaaatgtaa tatttatcaa tcatattttt gcttaccaac
      241 tgctgaacaa gatttgctcc gccacaaatc caaatatcct ttcctgcttc ttgttttagc
      301 cgttccaata ataaaacagg attttcactt gtaaaatgaa tttgttggga agaattgcac
      361 tctttatgtg ttaatacata agttgtaaat ttattatata cccattcttg cggagaaagt
      421 tcagtaacaa cctgatgaga agtattccac cccattaaga cagtatctat atctttggta
      481 aattctgtat aagtgtcgat gttttcattg tcatttccat gaccatttag ccaagctact
      541 ccaccattac cgtccgcaat atatccgtca aggctcatgg caataaataa aactactttt
      601 ctcatttttt ctcttttata aatacaaaca ggaatttatt tctctttttt actcataagg
      661 gaacaactga ttgctacaaa taaacccatt aaaatccatg gaaatgctgc tcttataaaa
      721 tcactaacca tactttattc ctccttaaat tccaatttcc ttagttctac aaactgaaat
      781 ttttgcctat gaatgtatct tgttctttat acgcaaacag gcttctgtgt gcataatcca
      841 atgtcttgaa aacggcattt gaattagtcc acaaatatct ttattacacc aataatcaat
      901 cagccaaatt gctttttcat tgtcgtttac tacattcaac gattctaagt atccttttct
      961 ttctttcggt atttttcttt tcaattcatc ataagataac cgttcaagca ttttctcagt
     1021 gctttcccat acttcgaaaa tatataaata taattcttcc agattaagct gttttgaaaa
     1081 gtctgcaatc tgctgtttca tgagttcatt tcctgttgta atgataggtg aattgatacg
     1141 ctcttgataa ttgcctgcaa aaaatacttg ttcatcttca tttatcactg tatgcacaac
     1201 tatatcttca atgcggaaaa tatgccaaat agaataagca atcgtcttac tatgataacc
     1261 atcagcgttt atgaagggga ttgagttaaa atcttctctg cataattctt ctttgaaaga
     1321 tactaaggtt tgtattaact gacttcgtaa agtaagtaaa gtatcaatac ccctcttata
     1381 agtatctttc ttttttattt gtgcctgcat tgttttatta agttcagacc attccttatt
     1441 catattcaat cctctctcaa attctgattt tatcgcccca tacaaactgg aatttgtctt
     1501 acaacaattt atatatgcat aaaaagcaat atatttatga aaacacccta aatccaaaat
     1561 ttactcaatt cattttttcc ttacataaaa tgttctcgta tatttcactt cctcaccaag
     1621 tgtttcccct atcaaaactt caaaatcatt gatatcaaat tctggaaaga atgtatctcc
     1681 atcttcaata tttaaatcta cttctgtgat atacatttta tctactattt gcaaagcttc
     1741 cttaaatagt ccatatccac cagatatgta tacatctcgt cctttagcca ataataatgc
     1801 atcttctaat gatttaactg aaactaaatt atctccttga tactctgttg tggtggaaac
     1861 aacaatattc attctattag gcaacggatg accgatttct tcataagact ttcgccccat
     1921 aataaccaca ttacccgttg ttaactctct aaattgcttt tgttctccct ttattttcca
     1981 tggtatatta ccattcttgc ctataacatt attctttgac ctcgcaacaa tcaaacctat
     2041 catctatttc atcctccata atatgatgtc gtacctgtga ttccatcaag tgcaccacat
     2101 cttaacaaac tcaaaattat ctcgaactaa ccagctcgac aaattccttt tttgtcttca
     2161 tctgcattat atcatattgt tataagcatt gccatttctt ttctgctttt gaaaagttac
     2221 ttatcagcct atgttttgta aagggtgata ttcagcattt ctcacgcaac aagcccttga
     2281 caaaccatag actaataagt tttaaggtaa tcaagcagaa atgaaatagt ctgctgtttc
     2341 acggcaacaa aaaagagata ccaacacact tatgtagtat ctcttagtct agtagcaata
     2401 ttcttttgtt tgcaagcagg taaacatggg ctgtttctta atatcttttt gtttgggaaa
     2461 cacctttttc ccatcctttc accgttgcca tctctgctgc gcacaatgcc acagggataa
     2521 accatagcaa accgcctaat aacaagaaaa atactaagct gaagcctgag gtcgcgaatg
     2581 tgggatattc atataatgaa acgaccattg agatggtcat agccaaaaag ccaaagaaga
     2641 aaaagtccaa tccttcttct tgttcttctt ttgtagtagt gatcgttgta aagtgcatcg
     2701 ccgattttaa aatcactgtt gtcagcacat aggcatacac ggcagtaatg atgacaccaa
     2761 tcacttgaat ccctaagaag taccagctgc ttggattcgt tagtaaatgg ctgttagatg
     2821 caaagatccc tatcagaatc gtacctgtga atccgcccat gccatgaaca ccccacacat
     2881 caagtgcatc atcccattgt ttctttttac gaaaatcaac agcagcatga cagacgatac
     2941 ccgcaatgat cccaatcagc atggcacttt ttgcttctac ataaccggca caaggcgtaa
     3001 tggctgccaa tccagcaacg gatcctgtta gtacgtcgac aaaatcaaag ccatttcctt
     3061 ttacttttgc aatgatcagc caagtaatca tcgcaaatgc aagagcgatg aatgttgaac
     3121 caaatgcatt gactgcttgt tcatctgctc gaagtgcacc accggaatta aaaccgaacc
     3181 agccaaacca aagcagtcct gtaccgatag cagctgccat aagactgcta tgctgacttc
     3241 tttctcctaa aggaatcttt ctttgaccaa gtgtgaggat acatgctaaa gagccgaatc
     3301 cagctgtcgt gtggatgact gctccaccag cgaaatctct aaatcccatc tgatccaaaa
     3361 atccgccacc ccatatccag tggcaaacag ggaaataaat cagcagattc caaaaaataa
     3421 caagtataat atagcctttt aaattcaatc tccctgcaaa agctcctgtc atcaaaggaa
     3481 cggtaatcac gataaacatc agttggtaaa gaaagaacat taaaaatgga atcgttgcac
     3541 catgaaacat gttggggaag cttgtaacat gtctcatcaa aaagaaatct gccgggtttc
     3601 caataacacc gccgatatcc cgtccaaagg ccaatccgaa tccaccaaat acccacataa
     3661 tcgttgttat cccaacggag agaaaagatt gagacatgat cgttagtata ttattcttcc
     3721 gtgctaatcc gccgtaaaaa aaagctagac caggagtcat caggcaaacc attgctgtac
     3781 ataaaatcat gaatgctgta tctcctgtat tgataggtaa tgtattcatt tttttcgcct
     3841 cctttctacg attacgagta taaaagaagg tgtagttgtt tttttcttct gtttttttga
     3901 attaatactt gcatttataa aagacagaca attaataaaa gcttgatgtg ttgtgctctt
     3961 tttcgttttt cacacaaata aagacaaaaa ataaacggtc ttctttttat agaaaaccgt
     4021 ttattttcgt ctttttttat ttttaaagat attttttcat aaggaataaa gaatacttca
     4081 tataaaatat ataaaaaaga accgcagcga gtgtcgctac ggttctcaaa tttgttcttc
     4141 atttcaacat aagtttttct tcaatttgct tataatttac gtgcaaagaa tgaaacgact
     4201 aatactaaaa tgattgcacc gatcaatgat ggaatgatag ccattcctgc aattgttggt
     4261 ccccaagttc ctagtaaagc ttgtccaatt gctgaaccta ctaaaccagc aatgatgttt
     4321 gcgatgatcc ccattgatga tcctttattc gtgattgctc ctgcaattgc tccaatgata
     4381 ccgccgacga ttaatgccca aataaatccc attttaaatt cctcctaact gacttcttac
     4441 ttcttattgt acacgtggtt cgttattttc tttgactttt tgtgcacctt tgcctacagc
     4501 agattttgct ttatctgttt ggcgtgaagc aaattgtcct gtggattttg ctgcttctgt
     4561 tactttgtct tgtactgttt cttgatcttt ttggtattct tcttgtgttt tgatgtctgc
     4621 aacattcaca ttcacttcga taacatctaa atgtgtcata ttgtttactt cagtactgat
     4681 caattctttg atttggttgt aaatatcttc gatgtcttta ccgtattcta cgatcacatc
     4741 tagatccaca gctacttgtt tcttacctac ttctgtattg atacctgctg ttacgttatc
     4801 tgtgttgact aatttttcag ccatatttga gaaaaatccg ccatctaatg tcaatagacc
     4861 atctacatct ttcaatgcat aaccgatgat tttttggatg actttgtctt cataagtcaa
     4921 ttcaccattc atcatgcggt tttctccttt ttcatttgcc aaaactgatc catttgttgt
     4981 attcatgttt ttgttttcca tctttgtttc ctcctatgat ttaaaaaatt gatcaactat
     5041 tcctgactgt ttcaaataaa aaccgattcc tgcgccaatt gctgtcatga ttatgatcaa
     5101 caatgtcttg aaaaatccaa tcgttaacca taaaacagct agcagtagtc caagaccccc
     5161 aaagataata gggagcttgt atgctgaaaa tagttctttc attatttggc cctcctattc
     5221 cactcgagag tgcttgtagt ctcttgtatc ttcttgttct tcataaccgc tgtatgtgac
     5281 tgccactttg accgtttctt ttgttccgag tactttagtt acttcttctt cgatatcttg
     5341 catcaataca cctgttttcc ctactaatga agaagttcgt ttcaagtctc cgttgacatg
     5401 gatatggatt cgatttttcg ttgaacgtat ccgtacttta ggtgaatgaa tgaactcttc
     5461 ttcgtgtaaa tgggaacgta ccaacccttc gatcgctttt ttgtcgattg ttagtttgcc
     5521 atcttcccgt ttcataacga aagttcctct tgactttgga tagaaaagaa cgactagcaa
     5581 gatgataata gcaagaacgg caaatactac agctgcccag aaaatatatt gttgcatgta
     5641 gtaattcgtt aaagtaaaga agcgaaatcg ttccaattgt tcaggcataa tatacggcgc
     5701 attgatcaac gcaatcgcta gaaatactga aagtagcagt aaactcatga taacaccgat
     5761 tatttttaac cccttattca ttttccacct cctataaact tatttttacc ctgtaaataa
     5821 ctctaccgac aatcacagta tagatagcga acagttagca tagcatcagt aaataaagcg
     5881 ctaccaaaca ttgctggatc ataacggcgt tttattgcca gtctgatact gaaataacaa
     5941 ttgcttactt attctctatt tacttttgat tgctggctat acttaaagga tattattcaa
     6001 gattaaaaat tgcaaagatt atgctcagtt attattacaa gattctattt atttattaaa
     6061 ttttttcgga gaattaaaaa atacaataaa taact
//
LOCUS       DANKKG010000138         6077 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000138 DANKKG010000000
VERSION     DANKKG010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6077
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.138"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            62..634
                     /locus_tag="PW004_002407"
     CDS             62..634
                     /locus_tag="PW004_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HDL2727340.1"
                     /translation="MTENKATQERTPQERLQEEQEHKEHVHHTKINAAAIADHLLGNM
                     HTLHVKLHQYHWYVKGANFFTLHEKFEELYNENEKWFDKLAERLITSGHKPASTTVEF
                     EKYSMLSEDAVNKYAKAEDMVENIIEDFRSTRDLTIRAIRLAQDEGDDALEDTLIAFK
                     NDLDKNIWMLQAFIGKEALEDDDALDEDED"
     gene            779..1483
                     /locus_tag="PW004_002408"
     CDS             779..1483
                     /locus_tag="PW004_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011476586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDL2727341.1"
                     /translation="MTKHTHHEIGDHAACIRLVPIFNHLEDSAMNYIAEKAHTKQYQK
                     GEFLFRSQEKEDTLYIINRGKIRIYRLADSGKEQLVRILNPGDFTGEWTLFNPDAVHE
                     EYAEATRDTVVCMIQQKDVQDFLEQYPAISLKLLAEMSKRLESSERQTTQVAVESVIT
                     RLVLFLAENVEPEMGNSPTITLPMAKKDIASYLGTTPETISRKFGELEDEGLIQQLSG
                     KRIKIQDLDDLLLYSE"
     gene            1711..2049
                     /locus_tag="PW004_002409"
     CDS             1711..2049
                     /locus_tag="PW004_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727342.1"
                     /translation="MHCFWVIVAITLSAAGAFFVVPFTKNTIGGEAKTSNVVEADRLA
                     MEKEEEVVKGAGVIGHGKVAYYEASDQGDKSVFTVWFPWSLAGILFSITMTRMTRKIE
                     EMEQIEWDSY"
     gene            2034..2888
                     /locus_tag="PW004_002410"
     CDS             2034..2888
                     /locus_tag="PW004_002410"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDL2727343.1"
                     /translation="MGFVLGVLNPHVVEIGSVVIHWYGVIIAAGILAALQLSTKEAKK
                     KGLEEDTIIDMALWAIPIGLLGARLYYVLFELGYYLQNPGQILAIWNGGIAIYGGLIA
                     GGGTLYWYAKKKGTSLALVLDILAPNVLLAQSIGRWGNFMNQEAHGGPVTRQFLENLY
                     LPEFIIEQMNMNGTYYHPTFLYESLWSLLGFVLIVTIRNRKQLLRQGEVALSYVLWYS
                     VGRFFIEGMRTDSLWIGEWIRVSQALSLALFIGAIVVWFIRRQDYPPISYYSDGNKGQ
                     KKTIKMVN"
     gene            3035..3268
                     /locus_tag="PW004_002411"
     CDS             3035..3268
                     /locus_tag="PW004_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HDL2727344.1"
                     /translation="MEKAILQLETLSCPSCMQKIEGAVKSVNGIDKDSIKVLFNSSKV
                     KTNFDSAVTSIEEIQKAIENVGYPVLKAKVKAV"
     gene            3466..5370
                     /locus_tag="PW004_002412"
     CDS             3466..5370
                     /locus_tag="PW004_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HDL2727345.1"
                     /translation="MQQYILSKKNVITVISGLLIVLGFFSHFVLENVGLSEWSLIIAS
                     VFGITPIAIQAFQAMKVKVISIDVLVSIAAIGALFIQNYEESAIVTFLFLFGHYLEQR
                     TLNQTRSAIKELTEMAPESALKQMDNGEFEEVEVDDIDEGDILLVKTGAKVPVDGIVL
                     TGEGHINEASITGEAIPVNKLADAEVFAGTILENGTIQIRADRVGEDTTFGKIIELVE
                     EAQDSKSEAERFIDRFSKYYTPAVLVLAIVVWAFSQNIELAITILVLGCPGALVIGVP
                     VSNVAGIGNGARNGVLLKGSEVIQDFSNVDTIVFDKTGTLTVGNPTVAATELYEKDPA
                     ETLGYLASVERESDHPLAKAVLNQIGETNFYPVEGTEVVKGGGIVSSVAGHRVAVGNV
                     ALMEKENVTLSKKVQKDVKRFEQQGNSLVLTAVDGELKVLMGIRDQVRPGVKANLQEL
                     KDLGVKNLVVLSGDNQGTVDVVAGELGLTEAHGYMLPEDKSAYIGKLQQRGQIVAFVG
                     DGVNDSPSLALADIGIAMGSGTDVAIETSDVVLMNSNFSNLPHALGLVKATANNMKQN
                     IVISIGVVLVLLTSVFFSEWMNMSIGMLVHEGSILVVIFNGMRLMKYKLKGRKEQKEV
                     SAPAEKVKIS"
     gene            5680..6042
                     /locus_tag="PW004_002413"
     CDS             5680..6042
                     /locus_tag="PW004_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2727346.1"
                     /translation="MEKVCQVTIVHKEKVNVIKKKLDQKDFSNLLVLGKCFSDSSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVFLKLSENLSMKS"
BASE COUNT         1969 a          999 c         1370 g         1739 t
ORIGIN      
        1 gaaaaaggag gaatcaaaga aagctatttt aaacaaacca aaattatagg aggaataaat
       61 catgactgaa aataaagcta ctcaagaaag aacaccccaa gaaagattgc aagaagaaca
      121 agaacacaag gaacatgttc atcatacgaa aattaatgca gcggccattg cggatcatct
      181 tttaggcaac atgcatacat tacatgtgaa actgcaccaa tatcactggt atgtaaaagg
      241 agccaatttc tttacgttgc atgaaaaatt tgaagagttg tataacgaaa atgaaaaatg
      301 gttcgacaaa cttgcggagc gtctaattac ttctgggcat aaacctgctt cgacaacggt
      361 tgaatttgaa aaatattcga tgctttcgga agatgcagtt aataaatacg ctaaagcaga
      421 agacatggtt gaaaatatta tcgaggactt cagaagcact cgtgacctaa ctattcgcgc
      481 gattcgttta gctcaagacg agggtgacga tgctttagaa gatacattga ttgcttttaa
      541 aaatgactta gacaagaaca tttggatgct gcaagcgttc attggtaaag aagcattaga
      601 agatgatgac gctcttgatg aggatgaaga ttaaggtaaa agtagaagct gatttaggga
      661 ttggaattct ttccagtcct ttttccattt tcttgatgtg catcaatttt tttgtagtaa
      721 gagacttata taataaaagc agcgaatata aacaaaataa ataaaggggg agaatgaatt
      781 gactaaacat acccatcatg aaattggcga ccacgcagcc tgtatccgct tggttccaat
      841 attcaatcac ttagaggata gcgcgatgaa ttacattgct gaaaaagccc acacgaaaca
      901 gtatcaaaaa ggagagtttc tgtttcgttc acaagaaaaa gaggatactt tatatattat
      961 caatcgtggg aaaatccgta tttatcgatt ggcagactct ggcaaagagc agctagtgcg
     1021 gatcttgaac cctggtgatt tcaccggaga atggaccttg ttcaacccgg atgcagtaca
     1081 cgaggaatat gccgaagcaa cccgagatac cgttgtctgt atgattcagc aaaaagatgt
     1141 ccaagatttt ctggaacaat acccagctat ttctttaaaa ctattggcgg aaatgtctaa
     1201 acggttagaa agttcagaac gtcaaacgac acaagtagcc gtggagagtg tcattacccg
     1261 tttagttttg tttttggcag aaaatgtgga acctgaaatg ggcaactctc ccaccatcac
     1321 cctgccgatg gcaaaaaagg acattgcttc ctatttagga acgacacctg agaccatcag
     1381 tcgcaaattt ggagaattgg aggacgaagg attgattcaa cagctgtctg ggaaaaggat
     1441 caagattcag gatttagatg atttattgct ttacagcgaa taatcggaca cacttttttc
     1501 tacacgttgg tgttctgtcg ctcttcatta acaacgggac agaactcagt gcttttgctt
     1561 ccatctgtcc gtttttggct tttttaagac agaacgacct tcattcattg gaaaaataaa
     1621 agcacaaaca tgataaccct caagtttttt tgttttattt ttctttaaga tagggttgtc
     1681 gaacaaaacg taaaaataag tagggtgaat gtgcattgtt tttgggtgat agttgccatc
     1741 acgttgtcag cagcgggggc attctttgtc gtccctttca ccaagaacac gattggcggc
     1801 gaagcaaaaa cgtctaacgt tgttgaggca gataggttag ctatggaaaa agaagaggaa
     1861 gttgtaaaag gagccggcgt aatcggacac ggaaaagtgg cgtattacga agcgagtgac
     1921 cagggagata aaagtgtatt cactgtttgg tttccatgga gcttagccgg aatcttgttc
     1981 agcatcacca tgactagaat gacgcgaaaa attgaagaaa tggagcaaat agaatgggat
     2041 tcgtattagg cgttttgaat ccccatgtgg tggagattgg ttctgttgtg attcattggt
     2101 atggagtcat tattgcagcg ggcatattag cggctctcca gttgagcacc aaggaagcaa
     2161 agaaaaaagg actggaagag gataccatta tcgatatggc cttatgggca attccgattg
     2221 gactcctcgg cgctcggctt tattatgtgt tatttgaact tggttattat ctgcaaaatc
     2281 caggacaaat ccttgcgatt tggaatggcg gcattgcgat ttatggggga ttgattgcag
     2341 gagggggtac gctatattgg tatgcaaaga aaaaaggtac ttctttggca ctcgttcttg
     2401 atattttagc gcccaatgta ttgttagccc agtccattgg tcgttggggc aattttatga
     2461 atcaggaagc tcatggtggg ccggttactc gtcaattctt ggaaaatctt tatttacctg
     2521 aatttatcat tgaacaaatg aatatgaatg gtacctacta ccatccgact tttttatacg
     2581 agtcattgtg gagtttgttg ggctttgtcc tcatcgtcac tatacggaac cgaaaacagc
     2641 ttttgcgtca aggagaagtc gctttgagtt acgttttgtg gtactcagtg ggtcggttct
     2701 tcattgaagg tatgcgaacg gacagtttat ggattggcga gtggatccgc gtttcgcaag
     2761 ccttgtctct ggccctgttt attggcgcga ttgtagtatg gtttatacgc agacaagatt
     2821 atccaccaat ttcttattat tctgatggca ataaggggca gaaaaagact attaagatgg
     2881 tgaattgagc aaaaagataa aaagaagtgc aaaacttgat atgcgtcaat tttcgtttgc
     2941 taaaagtgag ctatactaca gttgtaaaga ggaaaaggca gtgttcatta atctgctgcc
     3001 gataaaataa aaacacataa aggagatagg aattatggaa aaagctatat tgcaattaga
     3061 aacgttgtct tgtccaagtt gtatgcaaaa aattgaaggt gccgtgaaat cagttaacgg
     3121 tattgataaa gacagcatta aagtattatt caactccagc aaagtcaaaa ccaattttga
     3181 ttcagctgtc acttcgattg aagagattca aaaagctatc gaaaacgtag gctatccggt
     3241 tcttaaagca aaagtcaagg ccgtttaaca gattaaatga actaggcaat tctggaagaa
     3301 aagctatttt tccaggattg ctttttttgt tgcaatccga ttcgaggtgc ttagaaacga
     3361 taaaagagaa ctttctaaac ttgatgtcag tcaattaatt acttaggcaa ttatcttata
     3421 ctgtgtttat caataaaaga gaaattaaat ttggagggaa aaagcatgca acaatatata
     3481 ttaagcaaga aaaatgttat caccgtcatc agtggattgt taatcgtact cggtttcttc
     3541 agtcactttg ttttagaaaa cgtaggactt tcagagtggt ctttgatcat cgcctctgtc
     3601 tttgggatta cgccaattgc cattcaagcg tttcaagcaa tgaaagtcaa agtcatcagt
     3661 attgatgtct tagtcagtat tgcagcgatt ggtgcgcttt ttattcaaaa ttatgaagaa
     3721 tcggctattg tcacgttctt attcttattc ggacactact tggaacaacg aacattgaac
     3781 caaacgcgat ctgctatcaa agaattgact gaaatggcac ccgaaagcgc cttgaaacaa
     3841 atggataatg gcgaatttga agaagtagaa gtggatgata tagatgaagg ggatatcttg
     3901 ctcgttaaaa cgggtgcgaa agttccagta gatggcatag tccttacggg tgaagggcat
     3961 atcaatgaag ctagcattac aggtgaagct attccggtca acaagctagc tgatgcagaa
     4021 gtttttgcag gaaccatttt agaaaacgga acgattcaaa tcagagctga ccgagttggt
     4081 gaggacacca catttggaaa aattattgaa ctcgtggaag aagcacaaga ttctaaatcc
     4141 gaagcggaac ggttcattga tcgcttttct aaatactaca caccagctgt cttggttctg
     4201 gcaattgttg tatgggcgtt cagtcaaaat attgagttag caatcaccat cttggttcta
     4261 ggttgcccag gcgcattagt tatcggagtg cctgtctcaa acgttgccgg tattgggaat
     4321 ggtgctcgta acggtgttct tttaaaaggg agtgaagtca ttcaagactt cagtaatgtg
     4381 gatacaattg tatttgataa gacaggtact ttaactgtcg gaaacccaac cgttgcagcg
     4441 actgaactgt atgaaaaaga tcctgctgaa acgcttggct atctggccag tgtggaacgg
     4501 gaatcagatc atccattagc aaaagcggtc ttaaatcaaa ttggagaaac aaacttttat
     4561 cctgttgaag gaactgaagt tgtgaaaggc ggcggaatcg tttcaagtgt agctggacat
     4621 agagtggctg ttgggaatgt ggccttgatg gaaaaagaaa atgtcactct cagcaaaaaa
     4681 gtgcaaaaag atgtcaaacg gtttgaacaa caagggaact ctctcgtttt gacagcggtc
     4741 gacggtgaat taaaagtact gatgggcatt cgcgatcaag tccgtccggg tgtgaaagct
     4801 aatttgcagg aattaaaaga cttgggcgtg aaaaatctag tcgttctttc aggagataac
     4861 caaggaaccg ttgatgtggt cgccggcgaa cttggtttga ccgaagctca cggctacatg
     4921 ttgccggaag acaaatcggc ttatatcgga aaacttcaac aacgtggtca aattgtagcc
     4981 tttgttggag atggcgttaa cgatagtccg tccttagctt tagcagacat tggaatcgca
     5041 atgggaagcg gaacggacgt agcgattgaa acatccgatg tcgttttaat gaactcgaac
     5101 ttcagcaact tgcctcacgc attaggatta gtaaaagcca ccgcaaataa tatgaaacaa
     5161 aatatcgtga tttcaattgg agtagtgttg gtcttgttga ccagcgtctt cttcagcgaa
     5221 tggatgaata tgtctatcgg aatgttggtt catgaaggta gtatcttggt ggtaattttc
     5281 aatggcatga gattaatgaa gtataagttg aaaggtcgaa aagaacaaaa agaagtatca
     5341 gcacctgcag agaaagtaaa aatatcttaa gaataaatta gaagaggctg agacaatagt
     5401 ccatccttga aatgacaaaa gcggaaactc agatttttaa atctgggatt ttccgctttt
     5461 ttgagttctt gaaaaaaaat aagagtatag agtatgttct ttataaaatt aattttcgtg
     5521 taaatgatta aaaaatttaa taataaaatt taaattccta ttaagttgta acacatgaaa
     5581 ttgcttataa tagtgtattg aaattaaggt agattgacaa acgagtgatc aaatagatat
     5641 actaccttca aacgaccgtt tgaatgtgag gtgagatgaa tggaaaaagt ttgccaagtg
     5701 actatcgtac ataaagaaaa agtaaatgta ataaaaaaaa aattggatca aaaagatttt
     5761 tcaaacttac ttgtgttagg gaaatgtttc agcgattctt ctaggattaa aattttctat
     5821 gctttagaaa cctataagga aatgtgtgtc tgtgatttag cagaaatact tactgctagt
     5881 gtcgctacaa cgtcacacca tttacgtttt ttaaaaaaac atggaatggc aaaatcacgt
     5941 caagatggaa aagtagttta ttattcttta gcgaatgaag acatcctttc cgctattcgt
     6001 gtttttttaa agttatcaga aaatctatct atgaaatctt agtttggagg aagttatttt
     6061 gttacaaagt atttttt
//
LOCUS       DANKKG010000139         6065 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000139 DANKKG010000000
VERSION     DANKKG010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.139"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            8..853
                     /locus_tag="PW004_002414"
     CDS             8..853
                     /locus_tag="PW004_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL2727347.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1134..2354
                     /locus_tag="PW004_002415"
     CDS             1134..2354
                     /locus_tag="PW004_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HDL2727348.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2351..5071
                     /locus_tag="PW004_002416"
     CDS             2351..5071
                     /locus_tag="PW004_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2727349.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5055..5999
                     /locus_tag="PW004_002417"
     CDS             5055..5999
                     /locus_tag="PW004_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL2727350.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
BASE COUNT         2264 a          956 c         1187 g         1658 t
ORIGIN      
        1 aggagacatg gatgaaatga aactaattgg tatagattta gacggtactc ttttgaattc
       61 tgaccagagc attagtcaaa aaaatgcaag aacaatgaaa catttaccgg aagactgttt
      121 tccattcatt tgttctggaa gagaagtaga agacattaat aatattctca aaaaatctgg
      181 tttaaggata ccagcagtag ggttaaatgg ttctgttgga tttgatggag aaaaaaagat
      241 atttgagtct tccttcgata aaaatgatgt aaaaaaaatt gattctgtcc tatcgaaatt
      301 tccaaccaaa atatatacaa atctcggaag ttatgaatct cagaattata aaaataaaat
      361 gaaaaaaatt tttcacgagg tagggagtga attttctatc aaagagctaa actatgagct
      421 tgagtatgaa aaatctattt gttcaatccc ttataataat attgaggaaa ttatagaaaa
      481 ggatgctatc agcatttaca aattttttat ttttattcct aatcgtcaat tgaaagctaa
      541 tatatttaac catttgaaaa agatgctaaa tgtgtctgtt actgaatcgg cacctgtaaa
      601 tattgaaatc gtacctcaaa atgtatctaa aggtgtggtg tttgatcgtt tggaatcaat
      661 ctacaattta aagaaacctt tgagaatcgc gatcggagat agtttaaatg acttatctat
      721 gtttgaaagt gcagatatta gttttgcaat gggaaatagt catcaagaaa tcatgaaaaa
      781 agctgattat gtcactgttt caaatgataa agacggagta tctcaagcat ttgaaagaat
      841 tcttaccata taaactaatt aaggtagtat agaacataga attctaaata cggaaaaatg
      901 gagcataaat tccagtacaa atagtacttc ttcagtgtca aacgaagcga atataacata
      961 gatatttagg attgtgacaa aaatcatgtc acaatctttt tttcgaataa acttattttt
     1021 tgaagtcggc catttatcgt aatttctttg cataatacga acaaattttc ggttattgat
     1081 gaaaaaaaac attgaaaatg gtatgataaa agaaaaaaag gaggaaattt ttaatggtga
     1141 aatttatcca tgcggcagat ttacatatgg atcggtcatt tgaaggactg acaactcttg
     1201 ataaaactgt acaggaaaaa ctattgaaga cgaatctgac cgttttatca aatatcattg
     1261 aacaggcaat aataaataac gttgattttg ttttacttgc aggagataat tttcatcaaa
     1321 atcgtccttc attaaaaata cagaaacatt tttcagaaca gatgaaacgg ttagaaaaga
     1381 accatatacc tgtatttata atattcggaa atcatgattt ctatcaaaaa gagcggtatt
     1441 ggttctcctt tcctaaaaat gtccatttat ttacatccga aacggtagag acaaaaaaaa
     1501 taacgataaa aagtggagaa actgtctctc tcagtggatt tagttatcgt caaccgtgga
     1561 ttcaaaaaga caaagtgatg gaatttcctt ctagagaact cacagattac catattgggt
     1621 tgtaccacgg ggaacctggc gtctctcaaa aaggaaacta tgctcctttc caaccctcaa
     1681 agatgcaaga aaagggatac gattattggg ctttagggca tatccatgtg ccaacggtct
     1741 taaatgaaaa gcaaacaatt gtttatccag gtgcgcctca aggtcatact aaaaaggaac
     1801 aagcttctac atctattcta ctagttgaat taagtaaagg aagttgccaa atacatccga
     1861 ttaaagtagc agaggtctat tggaagacaa aggaaatatc attgaggaaa gcaagaacga
     1921 caaaagaggt cattgttcac attcgtcagc agctttcaaa agtagaggat ggatttagtt
     1981 taattgagat aaaattaaaa gattatgacc atctcaatac agatgttttg gaacggatac
     2041 aatcaggaga gctattagac tatttgttag aggaattctc agataggata aatgatttct
     2101 ttatttggcg tatttctctc atagagaata ctttttatac gaagacccca cttgctgctt
     2161 ctgctaaatt gatggaacag ttatttcaat actatcagac acctcaagat ttccaacaaa
     2221 ttttaaatga agtatatagt catcaagaag cggctagaat attgagcgaa ctgccggaat
     2281 atcaagaaga aacacttgaa aaagccaagc agctgcttaa tcaagatttt ttatttgagg
     2341 aggatcagga atgaagattt taaggatcga gattgctgct tttggaaaat ggcgtcaaaa
     2401 aagttttgac ttttattcag gcaatcagct gatctatggg ggaaacgaag caggaaaatc
     2461 aacgatttat caatttattc aagcaatatt atttggtttc ccttcaaaag gccgtaaaaa
     2521 aaaggattat acaccaaaag acggctcagc ctatggcggc aaaatatggc ttaaacatcc
     2581 tgtatatggt gaatttgctg tcgagcgcta caagcagcaa aatcgcggaa aatcgaaggt
     2641 gtggctgggg gatcaagtgg gaagtgatga gttactagaa aaactgatcg cacctttgac
     2701 gaaagagtta ttccagcagg tatttacttt ccagcaagag cagttgatgg aactagatca
     2761 tttgagagaa aaagagctac atgatgcgct gatttcttta ggcatcacgg gcagttctca
     2821 agtattccaa aagcggcaag aatattatca gaaagcacaa caattattca agaaaaaagg
     2881 ccaaaaatta ctaatcaatc aaaaattaaa tgaatggaaa gagctgcaag aaaaaattca
     2941 tcaaaaacaa gaacaggaag cttattttgg caagttgatc caacaagaac aggaatacga
     3001 gtcgcaacag cagcagttat tggcacaatt aaaagaggcg aacaaacaat tttcattagc
     3061 cagacagcaa gaacttaatt tttctttata tgaagaatgg caatctctca aaaaaaacag
     3121 cactacaggt cttcctgatg agcaacttct tttagatctg gagtctttct ccaaacggta
     3181 tcagcaaaca aatgagcgtt tggaagaatt agaagcaaca ctggaaaagc atagcgggat
     3241 ggatcagcag tcagccagat attattttta tttagagcag gagcaggaac taaaagatct
     3301 ccagcaactt caatttacag cggaacaatt aaacgaagaa tggcaaagat tacaaattga
     3361 gactagagaa acaaattcag aattgaagat tttggaacaa aggtggcatt ggcagaaaaa
     3421 tccgttaccg gaagaatttt tatatgaaga agaatggaag catcttctgg ctgaagaaag
     3481 aaggattgct gatctagaaa gccagatttc tgtgcgtctc caaatagcta aagaacagca
     3541 agagatgatt gaagcagaag tgaatgactt ggaagagacc tatccagcat tattacaaaa
     3601 tcagtcagag atagcaccag tctcttcatc taaaccgtat atgctgatgg ctggcggagc
     3661 aataatcatt gcaggtatat ttgtaccaat gcccttgaaa gtgatccttt tactaattgg
     3721 tgcaggcatg attggctatt tcttctacaa aaggcaacca aaaattaaaa ctaaaaatga
     3781 tttctctgag ataaaaggaa tgtggcaaga aaaactagga caacttgatc tagccttagc
     3841 tcaattagct gaagcagaag aaaaacaaaa gagaactgga gaagaaaaac agattcttca
     3901 gcaagaagta tatcgttttg cggatcatcg gcacttagga aagatggata cgctcaaact
     3961 catgaaagaa tatggacagg aagtcataga ttatcagaaa ttatatggcg tatttgagcg
     4021 gaaacataaa agagaacagg aaatacaaga aaaactacga caaatcgacc aaaaatttga
     4081 gtttgttaaa gaatggttac cgatacaaga taagacaatt tcagaaaaaa tggtattgct
     4141 ttcccgtttt atacaagaga tggaacagat gaaatttgcc agaagctatc agcaaaatac
     4201 tttattgaag caagaaatca atcaattgaa aaagaaacaa aaagtatgga tacaagaata
     4261 cagcgaacaa ttgactcaag caggcttatc ttatccttcg gaagtacctg tctttttaca
     4321 aaaagccaaa caagccgaac agcagatgaa aagatacaat gaattatcgg aaatgcttac
     4381 tccactattt cctgaagagc tgacaaaaga gaaattatct gtaagactgc gccaactgaa
     4441 agaaaagcaa tcccaactcc agcaagaaga aaaagaagca agtgaaaatc ggcaacgact
     4501 ccaacttcaa gtagaagagt tgcaaagaga cggcacattg gacgaacttt atcaacaagc
     4561 tagccggcaa aaagcaaaat tacaggaatt actggaggaa tggggagcac ttgaaatcgc
     4621 cgggacattc ttaggtgatc tttcaacaga gatgagtgaa agacaattac cccagttgtt
     4681 aagagcagca ggtgattatt taggtatttt aacaaaagga aattatcaga agatattact
     4741 gaaaaatgat acactgactt taacagacgg atatacagaa tttcccatct atgagttatc
     4801 tacaggtacg aaagatcagc tgattatggc gatgagattt gcttacctct cattagaagc
     4861 taaccgttcg atatgcccga tcatcattga tgatggctgg cttcattatg atcatcagcg
     4921 aaagtatcaa ttagctgaac ttttagctaa atttggagaa aagtatcaaa taatttgttt
     4981 ttcttctgat aaagaaatgg taagctatta ccatgagatg aaccaaatcg taaaacaatt
     5041 agaaggagca aataatgaaa aaaattcgtg atttagtaat cgatgagcag tttgaacaat
     5101 ttgtcctgat caaaagtgca gatgtaagaa tcgctaaaaa cggaaaaaaa tttattgctt
     5161 ttactttcca agatacttca ggtactatcg atggtaaata ctgggatgct tcggaggagg
     5221 aaatcaataa atttacagcc ggcacagtcg tttttctaaa cggtaaaaga gaggtttatc
     5281 aaggaaaccc tcaagtaaag attcttcata tgcgcttaac aagggtagat gagccaggag
     5341 agcctagttt atacatggaa agagcccctt tgaagaaaga agaaatggaa gaagaaatca
     5401 atcaattgtt gtttgagatc accaatgcgc attggaatcg tattgttcgc tatctactta
     5461 ataaatatca aaaacaattt tttgagtttc cggcagcaaa aagaaaccat catgcatttg
     5521 ccagtggatt agcttatcat actttgacaa tgcttcactt agggaaatca atcgccgatg
     5581 aatatcctga gctgaatcga gcattgttgt atgctggtat cattttgcat gatttaggaa
     5641 aggtgattga actttccggt cctatgtcga cagaatatac gcttgcagga aatttgatcg
     5701 gtcatctggt attgattgac gaagaaataa ctaaagcctg tcttgcgttg aaaatcagcg
     5761 aagctgacga agatgttctc gtattacgtc atatggtttt agcacaccat ggcttattag
     5821 aatatggctc acctgtacgt ccaagaataa tggaagcaga aatattacat caaatcgaca
     5881 atatcgatgc ttcgatgcag atgatgttga cttcgattcg tcaaacagaa ccaggaaaat
     5941 acaccgatcg gattttcggt atggacaacc gaagtttcta tgtaccaaaa gatttatagc
     6001 aaaaatttat ttttttcatt caaaacaaaa aaagcgagac ataagtcttc gctttttttg
     6061 ttttc
//
LOCUS       DANKKG010000140         6020 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000140 DANKKG010000000
VERSION     DANKKG010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.140"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(60..362)
                     /locus_tag="PW004_002418"
     CDS             complement(60..362)
                     /locus_tag="PW004_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727351.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(582..948)
                     /gene="ssrA"
                     /locus_tag="PW004_002419"
     tmRNA           complement(582..948)
                     /gene="ssrA"
                     /locus_tag="PW004_002419"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1119..1892)
                     /locus_tag="PW004_002420"
     CDS             complement(1119..1892)
                     /locus_tag="PW004_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL2727352.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            2036..2386
                     /locus_tag="PW004_002421"
     CDS             2036..2386
                     /locus_tag="PW004_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HDL2727353.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2367..3083
                     /locus_tag="PW004_002422"
     CDS             2367..3083
                     /locus_tag="PW004_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2727354.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            3083..4531
                     /locus_tag="PW004_002423"
     CDS             3083..4531
                     /locus_tag="PW004_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL2727355.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4602..5111)
                     /locus_tag="PW004_002424"
     CDS             complement(4602..5111)
                     /locus_tag="PW004_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727356.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
BASE COUNT         2046 a         1050 c         1169 g         1755 t
ORIGIN      
        1 cccttttata agtatggtga taaaatccgt tccgggctac cttttttagg tagcctactt
       61 taatctttat acctttctgg atcaacgaaa gtatacttca aatagtcata acgccgatga
      121 tcgctccgtg cgtctggcac atcagtcacg atatcaaaca aaaaatacac atctttcttc
      181 attctggttt ttgttgccgg aattttaaag tagttcttat tagagtaata gagattgatt
      241 aataagctat cttcgattgc taaaaagaaa acttctgaat cccacacctt ataaaaatct
      301 ttgacaaatc tattcgaagg atcaaattta aaccataatt gtgtttttcc ttccatcaac
      361 ataatgttca cctcaaaaag agtatacgaa ctaatgttct ttttgtaaag taggaatatc
      421 attgataata tggatggaac atatatatta aaaagtgttc tttttgaatg ctattttgaa
      481 tgccattttc attcatttaa cgaaaatctg cgaaaataaa aaatgctcaa acccttataa
      541 aataaggatt taagcatcta gcgaaatctg ctgaaatctc acaatggaga cggcgggagt
      601 cgaacccgcg tccaaacaca tcgccactta aaaatctacg ttcatagtca actcatttaa
      661 ggattcactt tacagcttgc cgagtgacag gcattctgta tcgctagtct gatgttctct
      721 ttttcaactt acagacggaa agttgaaacg tatcccacta aagttaggac ccttactcga
      781 gcacatgggc gatgccgaga ggatctacgc taactgtttt taggcagcta aagcgtaaga
      841 gttgttttcg tttttagcag ttatatttaa ctgtaacgtt tttacgtaga cgtaacctac
      901 gaaacgcaat tcaagctcga ctgtgcctgt cgaatccgta acgtccccgt tagctaacaa
      961 gggttacgta tatttttcat acaaaattag tatagcatat attgggataa attcaataaa
     1021 aaagcgaatc atatcccatt atttatataa taactgcccc tttcttaaga aaaaataagc
     1081 gattttctta agaaaagagg tagcttcaaa aaaagatctt aaatcagatc aaaatacttc
     1141 aatgcgtgga caattccgcc ttctgtattt ttctttgtga taaaggtaga aagttctttt
     1201 aatccgtcat aggcgttgcc catagcgatt ttgttgtcgc aagcttccaa caaggcaaaa
     1261 tcattcggac catcgccaaa tccaaaagta ggaacttttt ccagctgaag ttctttttgc
     1321 agacgtttga cacctgcccc ttttgagatt cctttcttca ctgtatcgat cgagtaagga
     1381 ccattccgat aaaaagttag ctcaggaaaa tgttcgtgat agtattcgtc acctgattga
     1441 gataagatca gcaacatatt tactgtgttt tctttaaagc ctaaaggatc aatttccgga
     1501 acatctgaat gaatgaagtc gtaagcattg atcattgttt ggctatgacc ggtacaccag
     1561 attttctgat cgttatagta agaaagttca tgaccgttca actgtacatg atcatacatt
     1621 ttttgacatt cttctgaaga aaatgtttga gaatagattt cttcgccgtc tactcgaatg
     1681 aaagaaccat tcatggtgac cgcagaagta atgcccgcat cttccatgat agagtggatt
     1741 tctgcttctg tacgtcctgt agcgatgacg gggagaacat tgttttcttt taatgcgttc
     1801 atagcttttg ccacttcggg tgtgattttt gatttcgcat ctagtaatgt tccatcaaga
     1861 tcaaagaaag tgattgcacg ataatttgac acaaataaaa ctcctttttt attggaataa
     1921 gtttatctta acaaagaatg aacaaaagac aaacctttcg tgaagaattc ctgaacatat
     1981 tggttcagtc aacaatttag agtatactat aagaagagaa tttaggaggg aaagaatgat
     2041 ttttttcagt attggtatct tattcttaat aattggcagt ctttttcgga ttctgccctc
     2101 aaaaggaaat ctgccttttt atgggtatca ttcgcctctt gccgcaaaaa cagacgctca
     2161 ttggcggctg gctcaaaaga caagcggaaa ctggtttttt cttatgggct tactgatggc
     2221 attgatcggc tattatttaa agacatctgg gcatacgaat tattttctga ttgaaatgct
     2281 tctgctagta tttccaatta tgcctatttt tattatgaca gaaaagaaat tacagaaata
     2341 tgacctggaa actggaggga acgataatga atattttaat gattgaagat aatcaatctg
     2401 tttctgaaat gatgcagatg tttttcttaa atgaaggttg ggaagcaact ttcaagtatg
     2461 atggaaaaga agggctcgat gcctttttag aaaacccaca acattgggat atgattacac
     2521 tagacctgaa tttgcccaca ctggacggta tgctagtagg aagagagatc cgcaaagttt
     2581 caagtactgt gccgattatc atgctgacag ctagagattc tgaaagcgat caggtgatcg
     2641 gcttagagat gggggcagat gattatgtca caaaaccttt cagtcctctg acgcttattg
     2701 ctcgaatgaa agctttgcat cgccgtagtg agttggcaga agcaagagaa gaagcagtag
     2761 tttcagatga tcattttgat atcgagacta gtcatttcaa gatgaatacg aaaacacgag
     2821 aagcgtatct ggacaaccgg ccaattgaag gactgacgcc taaagagttc gatctattgt
     2881 ttactttagc aaaaaaacca cgtcaagtat tctctcggga gcagttgctt gaattagtct
     2941 gggattatca gtactttggt gatgaacgga cagtggacgc ccatatcaag aaattacgac
     3001 aaaaaatcga aaaggtcgga ccacaagtca tccaaactgt ttggggagtc ggctacaaat
     3061 tcgatgattc tggtgttgcc taatgcgtta tttgtatcag caactcttag ctttctgcgg
     3121 aatggtcgcg ttgatcttat tgatcgttgg agtttctttt acacaactga caaaacaaac
     3181 gattgaagaa aacaattacc agcaactgtt tggatatgct gaatcagttg aaaagacaac
     3241 gcaaacctat gccgatacgc tcccgcagtt tagtcaagat caggcgtttc aaaatgcatt
     3301 gctgctgaca gagcaagtac tgacagaaca aaatgtgaac tttatattta tcgataaaaa
     3361 cgaacgtgtc atttatccga ctaatacagc tttactgaat tttaccttaa cagctgatca
     3421 atggaaaaat ctgaggaatg gacgtcaaga aaaattcaca tcgaataaaa atattttagg
     3481 acaagcacag gcgacctctt acgcacttgt gccatttaat ttgaatcatg aattctatgg
     3541 agcacttgta gttagtcaac ctgcgcgaaa tatcgataac agcgttcgtc caattacact
     3601 gaacctgttc aaaggattta ttttttccag tatcgttgca gttattgcca gctatgcttt
     3661 tgcagcattc caagttaagc gtatcaatcg gttaaggaat gcgacgaaag aagtgacgaa
     3721 tggtaatttt gatgtacagc tccccgtaca tgacaaagat gaatttgatg agttagcaga
     3781 tgatttcaat aaaatgacga attcattgaa agaatctcaa gcagaaattg aagaacaaga
     3841 aaatcgtcga agacaattta tggcagatgc ttctcacgag atgagaacac ctcttacgac
     3901 aatcaacgga cttctggaag ggttagaata caatgcaatc cctgaaaatc agcgagaaaa
     3961 cgcgattaaa ttgatgaaaa atgaaacaga gcgtctgatc cgtttggtga atgaaaactt
     4021 ggattatgaa aaaatccgta cgaatcaaat cagtatggtc attaaaaaat tcaatggaac
     4081 agaaacattg aaaaatattg ttgcacagtt agaagctaaa gcagaagcag caggagatac
     4141 attgacattg aaagccgttg atgatattga tgtttacgct gattatgacc gatttgtcca
     4201 aatcatggtc aatattattc aaaacgcgat acagtttact gaaaacggcc aaatcacgat
     4261 tactttagaa aaaggctatc ttgaaacgat catcacgatt gaagatacgg gaattggaat
     4321 gtctgaacaa caaatgaaaa gtatctggga tcgttattat aaagtagatc cttcaagaaa
     4381 aaatacaaaa tatggtgaat caggattagg tttaccaatc gtgcagcaac ttgtacgctt
     4441 gcataaagga aaattagaag tagaaagcga actaggaaaa ggaacaaaat tcgctgttcg
     4501 tttgcctgac gtagaaatca aaaaagagta aaaaacaagc ggtataaaag atcatgaatc
     4561 gatcttctat accgcttgtt ttttactata aaatgattga tttagcccgc gggctctaaa
     4621 cgatcaatat aaggactttg agcaatagta gcatcatgag taaagagtaa gataccttga
     4681 ttttcttcat gagctaaata agaaagcaag tggagtagct cttggatctc caatacacta
     4741 agttcatcaa atgtatcttc aataacaata ttgtttcgct tggaaagcaa ggcatgaagt
     4801 aattgcagct ttactttttc aaaagacgtc aactcagctg ccgcacaatt aagcaaggaa
     4861 ggagagatat caagtttttc ttgataggcc aaacgatctt gtttgatttc tttttccggt
     4921 acatccaaca ggacatggtc tttaagattc agataaggaa acatcggcca atttttttgg
     4981 atgatcgcaa aattatccag ttttgcaaca agaacctctt ttttcaagtg ctccaatatt
     5041 ttatgctgtt tttcgagagg acacaaaata acggtcaagt gttcagcatg aatcaaatat
     5101 tcaggtttca ttacgtccag gcctcctact ttgcaaatga tggataaaaa agtgaaaggg
     5161 aatagactaa gaatgagaca gcatccactg agcccaataa gagtttgggc aaatccctta
     5221 agtatatctg tgaaaaaaag atcattttgg aaatcgatgg taaaaaaagt ctgttgatcc
     5281 atcggaattg taagtccgct taccgaagtc aagatatcac ggatatctgg cagttcagta
     5341 gtctttaaga caagaaaatt gatcctttga agaagcgctt cataaactga ctggaagctt
     5401 acgatccaca ataaaaccaa gatacaacaa agaaagagag gaaagaacag ctgccagaaa
     5461 ataaaaagga ataatttggt tttagaaaga ccagtcaggt gccaattgat caattcctgc
     5521 ttgttttgcc tgactgaata gtaaaagaaa aagataaaaa ggaaggcaaa tagaaaaaat
     5581 aaactgaata tgccttttat ataaaatcgg ctagctaatt ctaaagccgg aagaagctct
     5641 gtagatgcag aagcgccaat aaatgaaagc cgatcttgga cttgattggc taaagccgat
     5701 tgcaaatggt aaagagtcag gcatgctatc aataaaaaag cagaaaagct tgaaaataaa
     5761 caatagagta ttgtctgctt tttgtaataa attaatcgag cagtagcata gcggattatt
     5821 ttcatacttt tcacctctat aaaaagtata gaaaatgaat atgaataaag tatgaaattc
     5881 ctttagtgaa atcacgaatt aaaaccagaa aatattttgt aaaaagcaag aggttgtgaa
     5941 gaagctgccc tattctatta ccgtagatgc aagcttttga acactgttta ttcgaaaaga
     6001 ggagcagcga caataatttt
//
LOCUS       DANKKG010000141         5919 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000141 DANKKG010000000
VERSION     DANKKG010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.141"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(103..645)
                     /locus_tag="PW004_002425"
     CDS             complement(103..645)
                     /locus_tag="PW004_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727357.1"
                     /translation="MSIKETTQITRQLNAIYKAAHLLQEHFVDKKVSFVGEVSTVAII
                     FSTTNFMHLCGIDYRRGTHLFFQDALDRKINLQDIQIKTDGTTFQKLQVIGSLDLLLG
                     KHISIVGRGVYSSLRYDAAIRTRKKILALSLKQNGLIYIPISLLNLSSKEIGPGQKVT
                     GIFSEDLTSGELKMIMEVID"
     gene            complement(958..1509)
                     /locus_tag="PW004_002426"
     CDS             complement(958..1509)
                     /locus_tag="PW004_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2727358.1"
                     /translation="MGYNVQPLRSQQEINDFLFCLRRNKNAERDVFLFLIGINSGLRM
                     SDIVKLKKKDVITSKNPRIVEQKTGKTRILYLSSLQDLIHGYTAPLDPEAYLFPSTKG
                     GHLEVNTVYQMFQKVAKLLGRDDIGTHTLRKTFGYHYYKKTKDVATLMEIFGHSSEKI
                     TKRYIGINEDEISETLLNFRLGF"
     gene            complement(1802..3733)
                     /locus_tag="PW004_002427"
     CDS             complement(1802..3733)
                     /locus_tag="PW004_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HDL2727359.1"
                     /translation="METIQEQQSQEKHNHNHNHDHDHDHGKMPVVLYFVGLALAVIAL
                     FLNEDYQLVQNVLFSIATISAGYHVILLEGLGETIENSKAQKKFIPNSHILMGLAAIG
                     ASLMGNFWEGTLLILIFSGAHFLEDYAEGRSKREITKLLEMNPTTARLILPDGSTKNV
                     DVSELKVGDQLQVLNGDQVPIDGVILSGSTSIDESSITGESIPKEKSKGDGVFGSTIN
                     GTGTFTMKVTKENKDTVFSKILQLVNQNQDNQTKAASIIQKFEPKYVTVVLIAIPLFM
                     LLAPFLLDWTWSQSVYRGLVLLVAASPCALAAATVSATLSTTSNLAKKGVLSKGSSYL
                     SQLADIKAIAFDKTGTLTKGKPEVTNYYFADSVNEENMIDIVVALEKESNHPLADAIL
                     RKFEQKNKLTIEVENQIGKGLTGDYKGRNYRIGKPTSFDDVSDEYSRLNNEWASEGKT
                     VVYVAVDEEVIGLIALMDVPSEYAKETIEYFRKQGIHTTLITGDSEMTGKAVAKQLGI
                     DEVIANVMPEDKSRIIDEQKEKYGVTAMVGDGVNDAPALVNADVGIAMGDGTDVAVEV
                     SDLVLMQNNLSKLVQSHNISSKMNRVIWQNIIFSMAVVAFLIVVSFLGLTDIAISVII
                     HEGSTLVVILNGLRLLKAV"
     gene            4032..4346
                     /locus_tag="PW004_002428"
     CDS             4032..4346
                     /locus_tag="PW004_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDL2727360.1"
                     /translation="MDIKTTSPIDKGSILSISKLFKVISDPTRLSVLFLLQKKECSVG
                     NIAISLDMEQSAISHQLKTLKDARLVKSRREGKSMLYSLDDLHVFSILEQVLTHVNEL
                     EK"
     gene            4388..5296
                     /locus_tag="PW004_002429"
     CDS             4388..5296
                     /locus_tag="PW004_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDL2727361.1"
                     /translation="MSKSDKDHNHSNQNQPKKNIKIAFFINLIFSISEFFFGFLFNSV
                     SIMSDAVHDLGDSISIGFAWFFQGYSEKKQDERFTFGYNRFSLLGALITGVVLISGSF
                     FMIYRSVPRLIDPQPVNYSGMFWLSLVAIALNGYAAWILSKGTSKNESMLNLHMLEDV
                     LGWIGVLIVSILMNFTEAYRLDPILSILIALYILYKTVPEFFTTMKILLNGSPENVNV
                     KSLADSIKNVPAVKDLSHFHIWSLDGEENALIITVLIDPSDIDKAREIKEEIAEVAHK
                     SSVENHITIEITTSKEELEKGYSHNT"
     gene            complement(5398..>5919)
                     /locus_tag="PW004_002430"
     CDS             complement(5398..>5919)
                     /locus_tag="PW004_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HDL2727362.1"
                     /translation="HTKKGIRHIFWGQYLGTGILVAVSLFAAYVLNFIPQDWIIGLLG
                     LIPIFLGIRVAFVGEEEEEEEEVVEKLESRGTNRLFWTVALITIASGGDNLGIYIPYF
                     TSLAFSEIIIALIVFAISVAVLCYISYKLAKISFVSETLEKYERIIVPIMFIALGIFI
                     MIENGTIQTLLGF"
BASE COUNT         1815 a         1162 c         1009 g         1933 t
ORIGIN      
        1 aataacccac atgaatgatt tgaaatcgtc ttaaaatgct ttattttata acccaaaata
       61 gaataattac ttcaaaatga cgtcgcatat cactattaat gattaatcga tgacttccat
      121 aatcatttta agttccccgg atgttaaatc ctcgctaaaa atgccagtaa ctttttgtcc
      181 aggtccaatt tcctttgatg ataggtttaa caaagagata gggatataga ttaatccgtt
      241 ttgtttcaaa gacagagcca atattttttt ccttgttcga atagctgcat catagcgaag
      301 agaagagtaa acacctctgc caacaataga aatatgcttc cccaataata aatcaaggct
      361 acctattact tgtaatttct gaaaggtcgt cccatccgtt ttgatttgaa tatcctgtaa
      421 atttattttc ctgtctaagg catcttgaaa aaacaagtgt gtgcctctgc gataatcaat
      481 gccacagagg tgcataaaat tagttgtcga aaaaataatc gctaccgttg agacttctcc
      541 gacaaaggat acttttttat ctacaaaatg ttcttgtaag agatgagcag ccttataaat
      601 cgcgtttaac tgtctggtaa tttgggttgt ctcttttata gacatgcgtt tgtattcttt
      661 ttttgccgcg tgcgaagaat gctccttttt cccaataaaa aaagctgctc aaaagagcag
      721 ccttttgtac aggtttagtt ttggtgcttg ccacccgccg gacccttacg ttccggattt
      781 gtatcgagtt tagttttggt gctcgccacc cgccggaccc ttatgttccg gatttatatc
      841 aagttgacta tgagcctgct ctcatttgta tatataatat accaaaaaaa ggttgatttg
      901 tccaatactg tgggtgatta taaccgtctt actgtgctta tctattatat aatacaatca
      961 aaaacctaac cgaaaattta ataacgtttc actgatttca tcttcattaa ttccgatgta
     1021 gcgtttggta atcttctcgc tgctgtgacc aaagatttcc attaaggtgg ccacgtcttt
     1081 ggttttcttg taatagtggt aaccaaaggt ttttcgtagg gtgtgggtgc cgatatcgtc
     1141 ccttcctaac agcttcgcga ccttttgaaa catctggtag accgtattga cttctaaatg
     1201 gccgccctta gtactgggaa acaagtaagc ctccggatct aagggtgcgg tatagccgtg
     1261 aatcaagtcc tgtaagctgc ttaagtataa aatgcgtgtt ttccctgtct tttgctccac
     1321 gatgcgcggg ttttttgagg taatcacgtc ttttttcttg agtttaacga tgtcggacat
     1381 gcgcagaccg ctgttaattc caattagaaa caagaaaacg tcacgttccg catttttatt
     1441 ccgtcgcagg caaaataaaa aatcattaat ctcctgttgc gaccgtagtg gctgaacatt
     1501 atagcccatg acaatccctc ctttttagtc tgttcgaata catgattata gttattacaa
     1561 cacgaaatcg tgtgtttagg gggagtaaaa aaaaagaacc ctataaggat aggattcttg
     1621 atacatgatt ttatccaatt tcatgtatct tatattcaac ccgcgccaca agagtatggt
     1681 tgatatatca ttttgacttt gaacatctag ataaatataa acaagtaata gagataaaaa
     1741 atgtagtgta atccttaatt atctctatta ctcgattaat ttgaagttgt gcaatcattc
     1801 tttagactgc ctttagcaat cgaagtccat tcaaaataac aaccaaagta cttccttcat
     1861 gaataatgac actgattgca atatccgtta agcctaagaa actaacgaca attaaaaaag
     1921 caacaacggc cattgaaaaa ataatattct gccaaataac acggttcatt ttcgaagaaa
     1981 tattatgaga ttgtaccaat ttagataaat tgttttgcat taaaactaaa tcagatacct
     2041 ctactgccac atcagtccca tctcccatag cgattcccac atctgcatta acaagagcag
     2101 gagcatcatt tacaccgtct ccaaccatgg cggtcacgcc gtatttttct ttttgttcgt
     2161 ctataattct ggatttgtct tccggcatga cattcgcaat cacttcatct attcctaatt
     2221 gtttagcaac cgcttttccc gtcatttctg agtcaccagt aattaatgtg gtgtgtatac
     2281 cttgtttcct aaaatattca atggtttctt tagcatattc actcggaacg tccataaggg
     2341 caataaggcc tataacctct tcatctactg ctacgtatac gactgtctta ccttctgacg
     2401 cccattcatt atttaaacga ctatattcat ctgaaacatc atcaaacgaa gtcggttttc
     2461 ctatacgata atttctccct ttgtaatctc ccgtcaatcc tttaccaatt tggttttcta
     2521 cttcaatagt tagtttgttt ttttgctcaa actttcttag aatagcatct gctaaggggt
     2581 gattggattc tttttcaaga gctactacga tatcaatcat gttttcttcg ttcacggaat
     2641 cagcaaaata ataattggtt acttcaggtt ttcctttggt caaggtgccc gtcttatcaa
     2701 atgcaattgc tttaatatcg gctaattgag acaggtaaga actacctttt gaaaggactc
     2761 cttttttggc taaattagaa gtcgtcgata gcgttgccga gacagtagct gctgctaaag
     2821 cacacggtga agctgcaact aaaaggacca atcctctata gacactttgt gaccatgtcc
     2881 aatcaagtag aaaaggagcc aataacatga atagaggaat ggcaattaaa acaactgtga
     2941 catatttcgg ttcaaatttt tggataatac tcgcagcttt tgtttggtta tcttggttct
     3001 gattcactaa ttgtaaaatt tttgaaaata ctgtatcttt gttttctttg gtgactttca
     3061 tcgtgaaggt acctgttcca ttgattgtac tcccgaagac gccatctcct ttggatttct
     3121 ctttcgggat actttctcca gtaatagacg actcatcaat ggatgtagat ccagacaaaa
     3181 tgacgccatc aataggtact tgatcgccat tcaacacttg aagttgatct cccactttta
     3241 attcactgac atccacattt tttgtactac catcaggcag gattaaccgg gcagtcgttg
     3301 gattcatttc gagtaatttc gttatttctc gtttgcttct tccttctgca taatcttcca
     3361 aaaaatgtgc gccagaaaaa ataagaatca ataatgttcc ttcccaaaaa ttccccatca
     3421 aagaagctcc aattgccgct aatcccatta aaatatgaga attaggtatg aacttttttt
     3481 gtgcttttga attctcaatt gtttctccaa ggccttcaag aagaataacg tgatacccgg
     3541 cactgattgt agcgattgag aacaaaacat tttgtaccaa ctgataatct tcattcaaaa
     3601 atagagcaat cactgctaat gctaaaccaa caaagtacaa aacgactggc attttcccat
     3661 gatcatggtc gtgatcgtgg ttgtgattat gattatgctt ttcttgagac tgttgttctt
     3721 ggatagtttc cacttctatt gcctccctat ataatttata attcctttgt tttccatcat
     3781 ttacttcttg aatcacataa atttctgcta tttctgactc ctttcgttta ttccaaaagt
     3841 aaatcgtaat gaatccacct tacatatgac taattaatca tatgtttgta tactcatttt
     3901 atatcgctat atacattttg tcaagataaa gagaacgcca cttctctttt ataaacaaaa
     3961 gattagcatt gtttcgctca tatggtaata tagatatgaa cggaaggtca tatgaaagga
     4021 ataaaaacac tatggatata aaaacaactt ctccaatcga taaaggatct attctgtcaa
     4081 taagcaaact atttaaggtg attagtgatc ctacacgatt gtcggttctc tttcttttac
     4141 agaaaaaaga gtgtagtgtt ggaaatatcg ctatttctct ggacatggaa cagtccgcta
     4201 tttctcatca attaaaaacg ttgaaagacg cacgcttagt gaaatcaaga agagaaggaa
     4261 aaagtatgct ttacagttta gatgaccttc atgtttttag tattctcgaa caagtcttaa
     4321 ctcatgttaa tgaattagaa aaataaaacc aattagtatg tagcatacaa acttaggagg
     4381 tttttaaatg tcaaaatcag ataaagacca taatcactcg aatcagaatc aaccgaaaaa
     4441 gaatattaaa attgctttct tcataaatct tattttctct ataagcgaat ttttctttgg
     4501 ctttcttttc aatagtgtgt ccattatgtc cgacgcagtt catgatttag gggactctat
     4561 ttcaattgga ttcgcttggt tttttcaagg ttattcagag aaaaaacaag atgaacggtt
     4621 cacttttggc tataaccgct tttctttact gggcgcgctg ataactggcg ttgtgctaat
     4681 tagtggctcg ttttttatga tctatcgtag tgtgccacgt ttgattgacc cacaacccgt
     4741 aaattatagt ggcatgttct ggctttctct cgtagctatt gcattgaatg gatatgctgc
     4801 gtggatttta agcaaaggca catccaaaaa cgagagcatg ttgaacctcc atatgctgga
     4861 agacgtatta gggtggattg gagtcttgat tgtgagtatt ctaatgaact ttaccgaggc
     4921 atatcgtctg gatcctatct tatcgatttt gattgcactt tatatcctct acaaaaccgt
     4981 tcctgaattt ttcaccacaa tgaaaatttt attgaatggt tcgcctgaaa atgtaaacgt
     5041 aaagagcttg gcggactcca ttaaaaatgt tcctgctgta aaagacctct cacattttca
     5101 catttggtca ctggacgggg aagaaaacgc ccttatcatc acggttctta tagacccttc
     5161 agacatagac aaggctaggg aaataaaaga agaaattgca gaagtcgcac acaaatcaag
     5221 cgtagagaat catatcacaa tagaaataac tacaagcaaa gaagagcttg aaaagggata
     5281 ctcccacaac acgtaagata gttctacgct aaaaaatact tcattatttg aaaagcaaaa
     5341 aaggcagcga ttttgattag tcgctgcctc tctttttatc tttcgtgatt atgattttta
     5401 aaaacctagt agcgtttgta tcgtcccgtt ctctatcata ataaaaattc ctaacgcaat
     5461 aaacattata gggacaatga ttcgttcgta tttttctaac gtttcggaaa caaaagaaat
     5521 cttggctaat ttgtaactga tatagcagag aaccgcaaca gagatagcga atacgattaa
     5581 agcaataatg atttctgaaa aagctaacga ggtaaagtat ggaatataaa tacctaaatt
     5641 atcgcctcca gaagcaatcg ttattaaggc aactgtccag aataagcgat tagttcctct
     5701 agattcgagt ttttcaacga cttcctcttc ttcttcctcc tcttcgccaa caaaagcgac
     5761 tctgattcct aagaaaatcg gaattagacc gagaagacca atgatccaat cttgcggaat
     5821 aaaatttagc acataagcag caaacaaact tacggctacc aaaatacctg ttccaagata
     5881 ttgaccccaa aaaatgtgac gaataccttt ttttgtgtg
//
LOCUS       DANKKG010000142         5885 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000142 DANKKG010000000
VERSION     DANKKG010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5885
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.142"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(<1..747)
                     /locus_tag="PW004_002431"
     CDS             complement(<1..747)
                     /locus_tag="PW004_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase family
                     protein"
                     /protein_id="HDL2727363.1"
                     /translation="MSEVTGYLEQVDNETYRLRAVLGYKPDGSAKRKSKTIKANSKRA
                     AYKELNIWLEQFEGMTDDSLDLFNITFGEFYRKIWLSEAEKNLEPKSYHNYKRMIENR
                     FLDKFDFIPLIDIKPYMIKKIVVNAQRINTKDPGRNSDKPLSRNTKLRMLYAVNNLFL
                     MAKNEYGAIKENPVENVKIPKEKGVKKNIEEPYSEEEIHAMLKAAFEESIEIKTLIVL
                     AFITGARQGEIAALEEKDIDFDKQEIRFHQR"
     gene            complement(865..1500)
                     /locus_tag="PW004_002432"
     CDS             complement(865..1500)
                     /locus_tag="PW004_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HDL2727364.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWVLIVLLFFGIGGALSGGE
                     NKDSATATSTSTNKETTKETTTKEEKTYKIGDDVAVGKMEYKVNSVEVVKQVGPSVLP
                     TNAKDTFLVVDLSVKNAGDKAVTVDSSFFKLKADGKTFEADSAASMSANQDENGNITN
                     SFFMESLNPDMQQTGKIVFDISEAQANAQNNVLQAQTGYFGTETVSIALHN"
     gene            complement(1583..2005)
                     /locus_tag="PW004_002433"
     CDS             complement(1583..2005)
                     /locus_tag="PW004_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HDL2727365.1"
                     /translation="MYLKLKEMLSEYNLKLIYMEMEEPGFYYPKPRIVFLNEKLHEDS
                     SEAFHLAHELGHFIASHFEYSVLYDNSTTFHSKFEAEADRIAIMILLNIFIENELTDE
                     SQFKLENFMEFYSINNKLRTECFNVCQSYFKKKYSYAQ"
     gene            complement(2023..2343)
                     /locus_tag="PW004_002434"
     CDS             complement(2023..2343)
                     /locus_tag="PW004_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2727366.1"
                     /translation="MTLFERIKSLASQRDKSMKEVALELGFSENLFYRWKTTEPKARD
                     LQKVADYFNVSVDYLLGREEKETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            2636..2827
                     /locus_tag="PW004_002435"
     CDS             2636..2827
                     /locus_tag="PW004_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727367.1"
                     /translation="MSNLDNGRAAIKKFMRENGVTMEDLATAYGYTRVRMQQIIDGHW
                     SGPKANKTILEIIRDYRIR"
     gene            2841..3092
                     /locus_tag="PW004_002436"
     CDS             2841..3092
                     /locus_tag="PW004_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2727368.1"
                     /translation="MEQLALVNLSALKASLAESEIANEVWDTKQAAEYLKTTTRTLTK
                     DAESGKIPAAKVGREWRFSSIALYQYLKGGKTWANSTEH"
     gene            3068..3247
                     /locus_tag="PW004_002437"
     CDS             3068..3247
                     /locus_tag="PW004_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727369.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGNRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(3290..3760)
                     /locus_tag="PW004_002438"
     CDS             complement(3290..3760)
                     /locus_tag="PW004_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727370.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            3847..4545
                     /locus_tag="PW004_002439"
     CDS             3847..4545
                     /locus_tag="PW004_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HDL2727371.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            4723..5064
                     /locus_tag="PW004_002440"
     CDS             4723..5064
                     /locus_tag="PW004_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727372.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            5057..5728
                     /locus_tag="PW004_002441"
     CDS             5057..5728
                     /locus_tag="PW004_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HDL2727373.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            5734..>5885
                     /locus_tag="PW004_002442"
     CDS             5734..>5885
                     /locus_tag="PW004_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727374.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIR"
BASE COUNT         1938 a          956 c         1059 g         1932 t
ORIGIN      
        1 ccgctgatga aatcttattt cttgtttgtc aaagtcaata tctttttctt ctaaagctgc
       61 aatttctcct tgtcttgctc ctgtaataaa tgctagaaca attaaagttt taatttctat
      121 gctttcctca aatgctgctt ttagcattgc atgaatttct tcttccgaat aaggttcttc
      181 gatattcttt tttacgcctt tttcttttgg tatttttaca ttttccacag gattttcttt
      241 aattgctccg tactcatttt tagccatcaa aaataagttg ttaacggcgt ataacatacg
      301 aagctttgta tttcttgata aaggtttatc agagtttctt ccaggatctt ttgtattgat
      361 tctttgagca ttaacaacaa ttttctttat catgtatggt tttatatcga ttagagggat
      421 aaaatcaaat ttatcaagaa atctattctc gatcattcgc ttatagttat gataagactt
      481 tggctctaaa tttttttcgg cctctgaaag ccatattttt ctataaaatt caccaaaagt
      541 tatattgaat aaatccaacg aatcatcggt cattccttcg aattgctcta accagatgtt
      601 caattcttta tatgccgctc ttttactatt cgcttttata gttttgcttt ttctttttgc
      661 cgagccatca ggtttgtacc ctaatacagc tcttagtcta tatgtttcat tgtctacttg
      721 ttctaagtat cctgttactt ctgacataat tgaatcagct ctctttctct gatacaatag
      781 gtactataaa gaagcctatt gtgtaggttt gttttttctt agaacacgct cgctttggtc
      841 ggtggggcgt gttttttata ctaattagtt atgaagagca attgaaactg tctctgttcc
      901 aaagtaacct gtttgtgctt gcaatacatt attttgtgca tttgcttggg cttcagagat
      961 atcaaaaact attttaccag tttgttgcat atcaggattt aagctttcca taaagaaaga
     1021 gttagtgata ttaccatttt catcttgatt tgccgacata gatgcagccg aatcagcttc
     1081 gaaagttttt ccatctgctt ttaatttgaa gaatgagcta tcaacagtca cagccttatc
     1141 acctgcattt tttacagata agtcaactac taagaaagtg tcttttgcgt tagtaggaag
     1201 tacagatggg ccaacttgtt ttactacttc aacggagttt actttgtatt ccattttccc
     1261 tacagcaacg tcatcaccga ttttataagt cttttcttct tttgttgtag tttcttttgt
     1321 agtttcttta ttagtacttg ttgatgttgc agtagctgaa tctttgtttt caccgccact
     1381 tagagcacca ccaataccaa aaaacaaaag tactattaat acccaaaacc atacccgttt
     1441 ataaaacggt tttttaacct tatacatttt cccatcttga cccataactt tttttgccat
     1501 ttaaatatcc ctcacttctt gttataatat atttgtgatc tcagaaatga ggtatgagtc
     1561 cgtgttgcag cacgggcttt ttttactgtg cataagagta ttttttcttg aaatatgact
     1621 ggcaaacatt aaaacattct gttcttaact tattattgat agagtagaat tccatgaaat
     1681 tttctaattt gaactgagat tcatcagtca attcgttctc aataaagata tttagtaaaa
     1741 tcataattgc gattctatca gcttcagctt cgaattttga atgaaaagtt gtagagttat
     1801 cgtacagtac tgaatattca aaatgtgaag caatgaaatg accgagctca tgggctaaat
     1861 gaaaagcttc agaactgtct tcgtgtagtt tttcattcaa aaatactatt cttggttttg
     1921 gataataaaa acctggttct tccatttcca tatagattaa ctttaaatta tactcactca
     1981 gcatttcttt caactttaaa tacatacaac ccatcactcc aactattcat tttcctctaa
     2041 agctttagca attgcaatcg ctttacgcat tgtctcctta gatatttctt ttccatcaaa
     2101 agaaaataca gtatcatctt ctgataaatc cacatgttta ggcgtttctt tttcttctct
     2161 acctagaaga tagtctacag agacattgaa atagtcagct accttttgca aatctcttgc
     2221 tttgggttct gttgttttcc atcgatagaa aagattttcg ctaaatccta attctaaagc
     2281 gacttctttc atgcttttat ctctttggct agctaatgat tttatccttt caaacagtgt
     2341 cataataatg ttctccaatc ctaaacaaat aaaagtacac aaaatgtaaa ttatactgtt
     2401 gacataagta taagtttgta ttattatttg tttgtaagtt aatttgatag aaaaaaagca
     2461 aactaaaaat acaccttata gcattaagtt tggcgaccga ttgcataata aagggtttgt
     2521 tgtaggctta tttaactatg tttatatagt atacgattgt atactattag tcaatagttt
     2581 atacgttttt tctatcattt tgacttacaa atctatatgt aaggggagtg aatagatgtc
     2641 aaatctagat aacggccgtg ctgcaatcaa gaagtttatg cgagaaaacg gagtaactat
     2701 ggaagatttg gctactgcat atggatacac acgtgttcga atgcagcaaa ttattgatgg
     2761 ccattggagt ggaccaaaag ctaataaaac tatcttagaa atcatccgtg attacagaat
     2821 tcgataggag gaattaatca atggaacaac tagctttagt taacctctca gccctcaaag
     2881 cttctttggc agaatctgag attgcaaatg aagtgtggga tacaaagcaa gctgcggaat
     2941 atttgaaaac cacgacacgc acgctaacta aagatgccga atctggaaag attccagcag
     3001 cgaaagttgg cagagagtgg aggtttagta gtatcgcttt gtatcagtat ctaaaaggag
     3061 gaaaaacatg ggcaaattca acagagcact agtattcagc gcaccgctaa tcatctacgc
     3121 tttagggctt tggggaaata gacaagcgtt gataggaacg attgtttaca tggtttggat
     3181 ttttatgggg cttgatgaag ctgaagctga gtacagagcg aaaaagccaa ccgagggggc
     3241 tgactaaaaa tgaatagaaa agagaaacta gaatgtatat tactattact cagtttaatt
     3301 ctttcactaa tttctctatt gggaagtttg tatttttgat atcaacaatt ttgtggattt
     3361 gggttctatt tagagtaaat atcatttttg tttggtgatt aaggagttta ttaaaattaa
     3421 ttttccctac ttcaaatgca aggtaacaag atatagcact tttaggagga atatttagcg
     3481 gtacagcttc agagtatatt ctattatcat cgactgatcc tcctttagca attaatttct
     3541 taaatggagt agctgaccat ctgcggttta agttagcgga tgaaagttct aaattaacta
     3601 acatagctgg ttctgttgaa tagttagaaa tgattacttt tgtataaatc atatctgcag
     3661 cgaagtaaga agcattaagt tcaacttcta gttgagggcg cattcctttt atttttatcc
     3721 ctgttaaaaa cgtactcaat ataaaactta ttattgacat ccattgaaaa attgttaagt
     3781 gaaaaaaatt caaaataaac accaccagtt tttaactaaa ttataccaaa aaggagagaa
     3841 gaaataatgc aagaattagt aattttgaaa aataaagaag ctgtgactac gagcttacaa
     3901 gtggcagaaa gcttcgagaa aaaacatcaa catgttttaa gagatattga tgcactaaaa
     3961 aaagatgtgt ccaattttgg acagatgttt gtagaaggta atgaaccaga ttcatatggc
     4021 agaaatcgac gagttttctt cattagtaga gatggatttt tcttgctggc tatggggttt
     4081 acaggaaaga aagctatcta cttcaaacaa aaatacattg aagcattcaa cgaaatggaa
     4141 gatgttattc gcaagaatac tgttcctcaa acaattgaag atatgatgat ctatcaatta
     4201 gaagaaatga aagatgttaa aaaagatgtt tccatgctta aagatactat gcgaattagc
     4261 ggacaacaag agtttgaaat taagcaaaaa ggaaatatga aagttatggg agttctaggg
     4321 ggaaaagaaa gccgagctta tgaagaaatc agcaaaaaag tattctcaaa attttggtct
     4381 gaatttaaac gtaccttttc aatcccaaga tatggcgagt tacctcgtaa gagattcgat
     4441 gatgctgttt catttattga aatgtggttg ccagaaactg cgatccgtat ggaaattgat
     4501 caactgaaca gacaacagag actttttggt gatgacaatg aatagagctg aagcgctaag
     4561 aatagggacg gcaattgcta attgctggtg gaaatactat aaaccaatca tcctaagcca
     4621 acaacatatt gacaagcaaa aagcatggca acaaataaaa aagtgactcc gccggcaagc
     4681 aaagagtcac aaagaaaaca catcataagg agattttagc atatggaaaa agaactttcc
     4741 actctagatc aatatttgat tgatcctgat tggggcaagc cgaaaattga ggaaacaagt
     4801 ggtcgaaaaa tcagacgaaa tcttttgacg aatgaagaac tagcttggga tcaagatgat
     4861 ttaggcaacc atgtaactat ttgggatcat gtttatctta tccatctatc gaagcattcg
     4921 aataaacctg aatatattta cgtcatcgaa gatggcttga ttgatgcgct agaagagtac
     4981 gacagagata acttgattga tatctcttat tacggaccag gtaagaaata cattgctgaa
     5041 atggaggcag aatttgatga gtgaaatcaa agggacaacg aactttgaaa aactttttag
     5101 tcgtaagtta aataaaattc tcaagaaaaa aggaaatttt gattatttat cttgggctca
     5161 cgcgtgggag attatgaaaa agaatgatcc acaggcaacg gtaactatta atgagtacaa
     5221 acactacagg gttgtttctg gaactcatca agactttctt gttgaggaat ataaaccttt
     5281 tcttatggat gaaactggga cttatgtatc tgtctcagta acggttaaag gacacacgga
     5341 aaccgaatta tttcctgttt tagattatcg aaaccaacca gttgttaaac caaatgcaat
     5401 gcaaatcaat aactcattga agcgatgctt tgtgaaagca ttggctctac acggactggg
     5461 attatatgta tttcaagggg aagatattcc aacaccacct agaatcgata caaagaaatt
     5521 aaacatgcta gagacgattc tagaagcttt caatgagcag atgggtaaag atatgaccaa
     5581 aaccttaatc gaatatgtta atgagcagac agataaatta gggctcttag ctgataacgt
     5641 tgaaactatt gaacagttaa gctatgagca atgtgccttg atggagcgag caatagcagc
     5701 taagagaaaa gaattagata agaagtgata tgagtgttta aaccattaat cgattcatat
     5761 tcagcggttc tgaaaaagtt caaaggaaaa gacataagcg caaccatcaa tgaggaagtg
     5821 aacattgatc gactaaagac gatgtatgac ggctacgatg gtgatcgagt cattgaaatt
     5881 cgttt
//
LOCUS       DANKKG010000143         5719 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000143 DANKKG010000000
VERSION     DANKKG010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.143"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(358..633)
                     /locus_tag="PW004_002443"
     CDS             complement(358..633)
                     /locus_tag="PW004_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HDL2727375.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            741..1505
                     /locus_tag="PW004_002444"
     CDS             741..1505
                     /locus_tag="PW004_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HDL2727376.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            1628..2131
                     /locus_tag="PW004_002445"
     CDS             1628..2131
                     /locus_tag="PW004_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL2727377.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            2540..3181
                     /locus_tag="PW004_002446"
     CDS             2540..3181
                     /locus_tag="PW004_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HDL2727378.1"
                     /translation="MEQTMKPYQYFFIKREVEQLLNAYSSVNDSKTVQTVQALAAEKI
                     RDVLGYEPSEIDTFLTTVLDVKLTKTQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDGKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKK"
     gene            3351..4619
                     /locus_tag="PW004_002447"
     CDS             3351..4619
                     /locus_tag="PW004_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HDL2727379.1"
                     /translation="MEKQLPSPVRILMYMLKWLVVTAILGVFMGSLSAFFLNSLTFVT
                     DIRLAHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNIAHLFRLDKAEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHAYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAVLA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAAYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRIKEKNKV"
     gene            complement(4646..5698)
                     /locus_tag="PW004_002448"
     CDS             complement(4646..5698)
                     /locus_tag="PW004_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDL2727380.1"
                     /translation="MLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLIS
                     FGIYLFVLLYETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSL
                     ANLLTLILAIFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLY
                     QFNRPRYNQDFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGG
                     QGADEKVPEAMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAI
                     FSSNNYHIFRAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICG
                     LLFAGSAFLSLITLLF"
BASE COUNT         1780 a         1016 c         1244 g         1679 t
ORIGIN      
        1 taaaaacttt tttgttctgt ctggaaaata tgggttaagc aaatttattg caagttatag
       61 atcagctttt ttactaccta tctttaatac ttgaggcaaa gcgtctcaaa aacaacctta
      121 tcgacggtgt tcaaaagtca gtttctcggt attaagtcaa attttatgca aaaatgtgga
      181 aagccatttt cacaaaattc tccttactac tcaaaactaa gcgactttct cacaacctta
      241 ttgacggtgt tcaaaagctt gcatctacgg cattaagtcc agcctgttga aaaatatgga
      301 gagctatttt tcacaacctg tccttaatgc ttgatgcaga ccagcttttt cacaacctta
      361 tcccaccaca tcaaatttct ttcgttcctc aatcatcggg tcttcttgga catccacata
      421 ctgcactcgt ccacttgggc tcggagattt cttgacttct tcaataaatt tctggatgat
      481 gtcgtcattg ccgattgctt cgatcgtgac tgagccgtca tcttcgtttt ttaccgttcc
      541 agtcacgcct agttgatcag cgaccatctt ggtcatgtag cgaaatccta cgccttggac
      601 gcgtccttgg acgttcattt taactttgcg catgttcgtc acctttcttt cctattttat
      661 aaattaatga taccgatttt tttaggatga aacaagctca agcacctatt ccatgatata
      721 atcatgggga ggtgaggaat gtgaaagaga tccaatcagc aaaaaatcca agaatcaaag
      781 aattaaaaaa actacataaa aagaaatatc gggaagaaaa ggaagaatat cttttggaag
      841 ggttccatct gattgaagaa gctgcgtcgt ctgatgcaga aataaaagaa ctttttgtga
      901 cacagcgagg catgaaagaa tggggagact ggctggccgc aaacaaagag atttctactt
      961 ttctagtttc ggatgaggtc atggatgctg tatcggatct acctacacca caaggaatct
     1021 tagcagtgat ggcgttaacg gctgaaggca cgccagattt ttcaggagga tggctgttat
     1081 tagataatgt acaagatcct ggaaatgtag ggacgatgat ccgtacggca gatgcatttg
     1141 atatggctgg tgttttatta ggcaaaggaa cggcagatat ttatagtaca aaggtgctgc
     1201 gcagcatgca gggaagcaat tatcatttgc ctgtgttgca aaaagatttg acagaagtga
     1261 ttcctttaat gaaagacaaa gggtttacag tgtatggcac agaattaaat gaagaagcac
     1321 acgccttaga caaagtagaa aaatcagata acttcgcaat aatcatgggg aacgaaggtc
     1381 agggggtaag tcaggaaatc ttgtctttga ctgatcagaa cgtatatatc ccgatgaaag
     1441 gaaatgcgga atcgttgaat gttggggttg cggcagggat tgttctttat catttttctt
     1501 tatgattaag gaactattaa gctttggcta ttttatccaa ggcgactaga ataatccgaa
     1561 cactggtggt atacttagat caatttcaga agaaaatgaa attcaaaaag gtggcagatc
     1621 gataattatg acagaaaaat ggcgtgaaga cgaagaatat atgtcttatg tagaagatct
     1681 tttagaaaca gaagcagtga aaaaattagc gaattacaca cagcatgttc attccactcg
     1741 tttggagcat tccatcagtg tttcttacta tagttatctg ctcgcaaaaa aatggggtgg
     1801 caatgctaaa gcaacagcgc gtgctggact tttgcatgat ctgttctact atgattggcg
     1861 tacgacgaaa tttgatgaag gcacacatgc gtatatccat ccaagaatcg ctgtaaagaa
     1921 tgcagaaaag atcaccgatt tgtcggatct tgagcgagat atcattttga agcacatgtg
     1981 gggcgcaacg attgcaccgc ctaagtacaa agaaggatat atcgtaacat tcgtagacaa
     2041 atactgcgca gtcaaagaag ccgctcagcc tatgtcagca agtatgcgta agagatggca
     2101 acgttacttc ggaaaagaat cctcgattta acaggtaaaa ccaataatag accatattga
     2161 ggttgtgaca gaagtgggtc agcttcaaga aataagaagg aatttacgaa aattgcttct
     2221 caaatttttg tgaaattccg gcttattttt tgaagaagct acttctggaa caccgtttgt
     2281 ttgtagaaag ggactttgac acgacttttg tcacagtcct ttttctgttt gcagttgact
     2341 atctgctcaa gatacggttt acttttattt atcgaatgaa tcatgtacat agaagaatga
     2401 acattaaagg gggtgatgat aagatgcaaa acgtatctta tttaaaagag tccgggatct
     2461 tcaggtaaca catactttgc agaagatcac ttttttctac aaagtatgca ctttggttag
     2521 aagaaaggac tttaaaatca tggaacaaac aatgaaacca tatcaatatt tttttataaa
     2581 aagagaagta gagcagttat taaatgcgta ctcatctgtc aatgactcaa aaacggtaca
     2641 aacggttcag gctctagcag ctgaaaagat tcgcgatgta ttaggttacg aaccatcaga
     2701 aatcgatacg tttttaacga cagttttaga tgtaaaactg acaaaaacgc aagcagaacg
     2761 agcattggag gagctgaagc aaatcgtcca gccgtttatg cagccgagca aaccacaaat
     2821 cgagaaatta ttccgaaaag tgaaaaaact caaacaaccc gcttggaatg aaatcgactt
     2881 gcaagcacat acgtacatcg gttggaatga tccagggacg caaaaaaaat tcatggttta
     2941 ttacgaagat ggaaaattaa aagggatcag cggtacttta tcaccaacga ttaataaggg
     3001 aatctgtgcg atctgtcaga aaacatcgaa tgtctcgctc tttctttcta caacgaaagc
     3061 cggttctgat ggcacgtata cgaaaaaggg gaattatatt tgtcatgata gtgatcaatg
     3121 taatcaacag ttaacgaaat tggctccttt ctatgaattt gctgaacgtg taaagaaata
     3181 acgataattt tattgttttt tacagaaaaa tatgctatct tttaatggaa gtgaataggt
     3241 gatggtgttc gcctttaaat gctaacgtaa gatagctaat gacgcctact aaagtctggg
     3301 gccagcccct ttctttagta ggcgtttttt tatttgagga ggaatttttt atggaaaaac
     3361 aattaccgag tccagtgcgt attttgatgt acatgttgaa gtggctggtg gtcacagcga
     3421 ttctcggagt attcatgggg agcttgtctg cttttttctt aaatagcctg acgttcgtga
     3481 cagatatccg actagcacat ccttggctac tttatctatt gccagtcagc ggcgcgcttt
     3541 ttgcttatct ttatgcgtat catggcggtc tttctagtcg tgggaataac ttagtgatcg
     3601 atcaagggaa tggaggagaa gagaagatcc ctttgcggct cattccattg accttgtttg
     3661 ggaccatcac tacccattta tttgggggat ctgttggtcg tgaaggaaca gcagttcaaa
     3721 tggggggagc attagctgat aatatagcgc atttattccg attggataaa gcagaacggg
     3781 agattttggt tatcagtgga atcagtgcgg gttttagttc tgtatttggc acacctttag
     3841 ctggaacatt atttggatta gaagtgttgg caatcggtaa agtccgaaca gaagcaatat
     3901 ttcctagctt ctttgctgcg ctttttgcaa atttcatgac agaaagctat ggtgtttctc
     3961 atctacatta ccaaatgggt gtgattcctg aatggagtat gttgctgttc ttcaaagttt
     4021 ttgttgctgg gattgctttt ggtttgattg gttgggtatt cagccgttcg atcgtctttt
     4081 taaaagcgcg gtatgcgcaa tggattcctc atcctgtttg gcgaaatacg ataggtggag
     4141 cagtagttgt agccgcagcg ttgattttgc aaacacaacg ttaccttggg ttgagtcttc
     4201 cgttactgca ggaatcattt aatggacagg cacatgctta tgattttatt gggaaattat
     4261 tcttcacggt cttgtcttta ggtgcggggt accaaggggg agaagtgaca ccactatttg
     4321 agattggtgc aacgcttggc gcagtactag cgcctttgct ccatgtttct gttccttttc
     4381 ttgctggatt aggttttatc ggggtatttt ctggtgcgac aaatacaccg attgcctgtt
     4441 ttgtgatggg aatcgaatta tttggttcac aagctgctgc ttacttcttt atgatctgct
     4501 tgatcagctt tatgtgttca ggaaacagcg gtatttattc tgcacagcaa gtagccgtca
     4561 aaaaaggaat actattcttg ccaatcgtca agagaataaa agaaaaaaac aaggtgtgac
     4621 atatgttttg tcacaccttg tttttttaga aaagtaaagt gatcagtgat aagaatgcgg
     4681 aacctgcgaa aagcaatccg caaatgatca tatgtctttt tttgttcatc atcaagatgg
     4741 cgatgtattc tcttaaaaac gcgttaggca agtaatagaa agctgttttt gccccaatgc
     4801 catttgcctt cagtttcgct tgtcgtgcat agagaccagc tctgaaaatg tggtaattgt
     4861 tggaactgaa gatcgcgcga tagggacctt tcatttgctg atccatgatt tcctttgaat
     4921 agcgcatatt ctccaaagtg gttgtcgaat tggtttcgac aagaatatct ctttcaggaa
     4981 ttccttgttc catggcatat tgtttcatcg ccattgcttc tggtactttt tcgtctgctc
     5041 cctgtccacc cgacattaat aggataggcg gatttaatgt cgcacggctt tgtgcccaat
     5101 aaaaagcaat cgccttattg attcgcttgg caagaagagg agaaactgtt tctccattga
     5161 tcaacccggc gcctaacaca acgatgaagt cttggttgta tcgagggcgg ttgaactgat
     5221 acaagaatga gacagtcaaa aagttgtaga atacgataaa tagatagatc aagatcaatg
     5281 ggatgcagaa aaacaaagca tttatccatt gtggtaaata ttttaacaag aaaaagtcaa
     5341 aaacaagaaa caatgtaaga aaaatggcta gtatcaacgt taaaagatta gctaatgaat
     5401 gcgactctct acgtaaaact gtgacaccgt tccaaaaaag aaaaacgatc aatccataaa
     5461 tgccgaatag aaagacaagc agtaacggga tcgtgatcaa ggcaatcagt ccgccaagaa
     5521 aaatattttg tgtttcgtat agcaagacaa acaagtagat accaaaacta atgaggaaaa
     5581 tattgaataa aagaccattc aacagccttc gcttttctga aaaataggaa aaggcaaaga
     5641 tactgaaaaa cactaaggga atcaaaaaat aaaaggtcga ataaaaaatt tccaacacga
     5701 caaaataagt caagattac
//
LOCUS       DANKKG010000144         5706 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000144 DANKKG010000000
VERSION     DANKKG010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.144"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(<1..610)
                     /locus_tag="PW004_002449"
     CDS             complement(<1..610)
                     /locus_tag="PW004_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HDL2727381.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQK"
     gene            complement(660..2156)
                     /locus_tag="PW004_002450"
     CDS             complement(660..2156)
                     /locus_tag="PW004_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL2727382.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(2350..3972)
                     /locus_tag="PW004_002451"
     CDS             complement(2350..3972)
                     /locus_tag="PW004_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDL2727383.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(4300..5613)
                     /locus_tag="PW004_002452"
     CDS             complement(4300..5613)
                     /locus_tag="PW004_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDL2727384.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
BASE COUNT         1738 a         1231 c          922 g         1815 t
ORIGIN      
        1 tcttttggac agtcggcatg atattggaaa aaattgtact gatttgttga ggaaaccttg
       61 cgtgttggat caccgtttct gttccaacaa gagataagaa aattcttttt gaattttctt
      121 cagcaatgat ttttagtcct tttgtcgacg aaacttccag ttctaattta ggattgcgct
      181 cgatccaacg caaaatcgtt tttatttgta atgatacggc tgtttttgaa agaaagaaaa
      241 tgtctgctat cttttgcata ctgacatacc ctttttgaaa caataagtac gtcacaatag
      301 ttgaagctcg gaaatccatg ctggaataga ttttatgttt cgtcacctct tgtatgattt
      361 gctgtttagt ttcagattca attgattcta aaaaataacc tgaacgttta tttgactgga
      421 taatgatttt cttctcgtag atagcaagca tatttttgat ttcagtttgg atcgttttct
      481 tgttggtacc taaagcttca gctaattctt cgcttgttat ccaatgattc ttatttctta
      541 atactacaag aatttggagt tgtcgtttat ttaagtccat ttgcttacac ctcctctttg
      601 ttataaacat acaggaaaaa cctctcaaat gacaatagtc acttgcagag gtttaaaaat
      661 catttgaaga aagatctata ttcaaggttt ttaccattag actggataat ttgtttataa
      721 tattcaaaac tatctttttt caatctccta aagcttccgc tcccatcatt ttgtctatcc
      781 acataaataa agccatatcg cttcctcatt tcccctgttc ctgccgatac gatatcgatt
      841 ggtccccacc ataagtaacc aatgatttgg caaccatctt ttattgcctc agcaatttcc
      901 tgaagatgta tttttagata ctcacaacgt tttggatcgt ggattcgatt caattcatct
      961 ggtgcttcat ctagtccaat gccattttca actatgaata atggtaattc ataacggtct
     1021 gtcaattcat tcatgacata acgaaaccct agaggatcaa ttggccaact ccaaggttcg
     1081 ggagattttt ccttcaaaaa gggattatcg aaaccatttg ctccgccagt atctacgcgc
     1141 atttctgctt cttttttata gacagcgcta cgataataac taaatgcaat ataatctgcc
     1201 gtgttagctt gcaagcactg ttcgtctcca tctaacatta ctggtgcaca gtcttcttcc
     1261 ttccatgtcc tgtaaatata agctggataa tgccctcggc acatgacatc ggagaaaaac
     1321 catgaattgc gtttaaattg cagtgaacca aatacattct ctggatcaca gtcatatgca
     1381 taagtcgcta atgcagaaaa gctacacata atacccatct tagcatttgg atcgattttc
     1441 tttaataatt gagtagcacg ggcgttcgct agaaacatat gatgacaggc ttgataaatc
     1501 actttttttg ttagtgtagc aatgggttca agtttatctc tagcagggaa tgtaaccaat
     1561 ccagcagctg cataaggcat acgaaaagca ttattgatct cattaaaggt catccaatat
     1621 ttcactttcc cttggtagcg ttcaaagacg gtctgcgcat aaaattcaaa aaagtcgatt
     1681 aattttcgat tttgccaccc cccatactct cctaccaatg ctaagggggt ttcataatga
     1741 ctaagagtaa ttactggctc gatatcatat cgatttaatg tattgattag tctgtcataa
     1801 aatacgagac cagcctcatt tggcttctgt tcgtctcctc ttggaaatat tcgtgcccat
     1861 gagatgcttg ttcgaaaagc ttttaaacct aactccttca gtaaacgaat atcctcttca
     1921 aatcgatgat aaaaatcaat cgcttcatga gaaagataat ggagtgattc gtctaaagca
     1981 atctcccaaa tctttttttc ttcattccac tgaatactag gatgctggtt catgatcccg
     2041 ataagcgtat ctgtcacatt aggtaatttg ccgtcttcca accatgcacc ttcacattga
     2101 ttagcagcta ctgctccgcc ccataaaaaa ttttctggaa atatttcctg atccataact
     2161 atctctcctc gctatcaaca atttcttact tgaagtatag ctcgattcat caaaacaaga
     2221 tattctaact atttcggcag aagagaagaa aaaaattgaa tgctactctt catttttaat
     2281 atttatatat aacaaataca taagacgatc cccgaaagaa aacactcgtt aaccatctaa
     2341 gcaaacgatt tattttactt ccaacacaaa tgcttcataa ggtcgtaaat caactttgcc
     2401 gtctggtaac tgtttcagag gataatttga cacaataacc cttaaagctt cacgtggaat
     2461 agtgaacgtc tgcttttcac tggacatatt acaaataacg agccattgct ggtcttcata
     2521 tatgcgagaa taggcaaaga catgctcagg cgtcttttcc actaattcat agtctcccca
     2581 gacgatgatg tcatgttttc tacgcaaatc gatcaatttc ttgtacatat aaaaaacaga
     2641 atcttgatct gctaacgctt cttctgcatt gatttcttta taatttggat tgactcgaag
     2701 ccatggaatt ccagtagtaa acccagcatg ttcacgggcg ttccattgca taggcgtcct
     2761 agcattgtct cttcctttcg ccttgattga ttccaagatt tcctcttttg taaatccaga
     2821 agaaatcctt tcgttataca tattgattgt ctcaatatct tttgcttccg cgatatcatc
     2881 gattgggcaa ttcgtcatgc ctaactcttc tccttgatag atataagggg tgcctttcat
     2941 catatggagc aagatagcaa acatcttggc actttctttt cgatacacat cgtcatttcc
     3001 ccaacgagaa acaattcgcg gaagatcgtg attattccaa aataaactat tccatccttc
     3061 atcacctaaa gatgtttgcc atttggataa tacttttttc agttcagaga tactcaaggg
     3121 tttcaaatcc catttgtctt tgccttcttg ctgatctaat cctatgtgtt cgaattggaa
     3181 gaccatggat aattcatttc ttttaggatt ggaataaagt ttagcgattt cggtagttgc
     3241 tccccatgtt tcgccaacag tcatcagatt tttgtcacca aaagttgctt gattcatttc
     3301 ctgaagatac tcatgcagtt taggtccatt acttgtgatt tctttatccg gttctttgcc
     3361 aatcatatcg atcacgtcca tacgaaagcc cccaatacct ttatcgatcc agaaattcat
     3421 catttgataa atctcttggc gaacctgttt atttttccaa ttcaaatcag gttgacgctt
     3481 gctatacaag tgcagaaaat attgccccgt tttttcatcc aactgccaag cagaaccact
     3541 gaatattgag cgcaatctat taggctcttt tccattgacc ggatcacgcc aaatataata
     3601 gtcccgataa ggattttctt tatttttacg ggcctcaatg aaccacggat gttcatcaga
     3661 agtatgattc accaccagat ccatgatgat acggatattt cttttctttg cttcttcaat
     3721 cagttcttcc atttcttcca tcgtaccgaa ttctgccatg attgcttgat aattactgat
     3781 atcataccca ttgtcatcat tcggagactc gtatactgga gaaagccaaa ttgcatcaat
     3841 acctaaatac gccagataat ccaacctgga aataatcccc cgcaaatctc caataccatc
     3901 ttgatttgaa tcttgaaaac ttcttggata gatttgataa acaactgaat ggtgccacca
     3961 tttttgttcc atgattttcc tcctataaat ggatgtttct attttttgct taatcattta
     4021 ttttagtctc tctcactaaa aattgtaacg ctttcaaata gctattgcac cttgttatcg
     4081 gtaacagaat aaaacaaaaa aacagctgag gaaattccct caactgtttt ttgaaaggat
     4141 tgatttatgc agggaagaat gctgatttca caacttcttt ataaacggtg ttcactcagc
     4201 tgtcccttgg tattagctca aaaaacggca aaatataaga aactattttt tcgttttttt
     4261 ctctaatact caggccagaa cgctgatttc acaacctctt tatttcacat tcaatatcca
     4321 catgaatccc acaaatacaa agaacagacc atacatgatt ggagatactt cttttcctct
     4381 tttagctgct aacatggtaa ttggatagaa gataaaacct aatgcaatcc catctgaaat
     4441 actataagtc aaaggcatgc cgatcaagat caagaatgaa gggatcgcaa tctctaagtt
     4501 tccccattcg atttgtcgca aagattgtgc catcaatact cctaccataa tcaatgctgg
     4561 tgcagttact tgtgaagtaa caactgatag taatggagaa aagaacaagc ctaacaagaa
     4621 gaaaatccct gtagtaatag ctgtgatacc tgaacgtcct ccaacggcga taccagctga
     4681 tgattctaca aatgcaccaa caggagaagt accaaataag gaacccgcaa gcatagctgt
     4741 tgaatcagaa gctaaagctt tccctacacg aggcatcttg ttgtctttca tgaaacctgc
     4801 ttggtttgct aatccaacta aagttcctgc tgtatcaaag aaagtcacaa gcaagaatgt
     4861 cagcactacg acccacattt gaagagaatt gatgtcccct acatgtttta atgcaactaa
     4921 aaatgttggt ttcaaacttg gtgctgcggc aacaatttca gaaggcattg gaatcaaacc
     4981 tgtgaccaga cctaaaatcg ttgtagctgc catcccgatg aagatgccac ctggtacacg
     5041 ccgaacaagc aagatagcag ttacgactaa tccaaaaatc gtcaaccaag tagaaccaac
     5101 gttgagtgat cctaatccta ctaatgtaga ttcattcgaa acgatgatcc caccttcgct
     5161 cagaccaagg aaagcgataa atagaccgat accgccagaa atcgcatatt tcaaatctgc
     5221 tgggatcgca tcaatgatca tttcccgcag cttgaagatt gtgatcaaaa taaaaatcaa
     5281 agaagcaacg aatacgccgg ctaatgctgt ttcccatgga atccccattc ctaaacaaac
     5341 agaataagca aaaaaagcat tgatcccaag agcaggtgcc gttccaattg gataacgggc
     5401 taataccccc attaaaacac agccgatcgc actggctaat gcagtcgcag taaacactgc
     5461 tccttcatcc attccagaag cacctaatac agttggattt acaaataaga tataagccat
     5521 tgagataaat gttgtaaatc ctgccagtac ctcacgtttt accgtggtgt tcaatttttc
     5581 tatttcgaag taggaaagta ttttttcctt catcttccat ccccctaaat ccgaacatta
     5641 taaataaagt ccgttaaaat ttcgatttat taataaaaca acgtgtttta tcatatacta
     5701 aaacaa
//
LOCUS       DANKKG010000145         5656 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000145 DANKKG010000000
VERSION     DANKKG010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.145"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            44..1675
                     /locus_tag="PW004_002453"
     CDS             44..1675
                     /locus_tag="PW004_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HDL2727385.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            1678..2757
                     /gene="uxuA"
                     /locus_tag="PW004_002454"
     CDS             1678..2757
                     /gene="uxuA"
                     /locus_tag="PW004_002454"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDL2727386.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            2831..4258
                     /gene="uxaC"
                     /locus_tag="PW004_002455"
     CDS             2831..4258
                     /gene="uxaC"
                     /locus_tag="PW004_002455"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HDL2727387.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            4342..4989
                     /locus_tag="PW004_002456"
     CDS             4342..4989
                     /locus_tag="PW004_002456"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HDL2727388.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            5109..>5656
                     /locus_tag="PW004_002457"
     CDS             5109..>5656
                     /locus_tag="PW004_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2727389.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQ"
BASE COUNT         1937 a          772 c         1191 g         1756 t
ORIGIN      
        1 taaaatgtat caaaactaac atatcagttt gaggaggtta attatggttt cgattaaaaa
       61 ttggcaaaat tctatcgaag aattcaatga aatgcagatt ctactgccgt gttttgatgt
      121 caaagaagtt aagcaagaag gccagcaaca tccagtatgg cttcattttg gtggaggaaa
      181 tctttatcga ggatttcatg cagaaattgc tcaatgctta gcagatcaaa atgaattaga
      241 aacaggagta gttgtgtgtg aaacatttga tgaagaagta gtggaaaaag cttatcatga
      301 ctataacaat gacattttgg aaattgttat gagtggagat ggtgaactca aaaaaagact
      361 tttggcagta acggctgata gtttatattg caatccgaag aatatagcgg actatcaaag
      421 agtggttaaa tattttgaag atcctaaatt acagtttaca acgtttacta ttactgaaaa
      481 aggttacagt ttaaaagatt ctaaagggca attcttttcg gtagtcaaag ctgatatcaa
      541 atctggacct agcaatcctc aacatacgat gagtattgta acatccttgt tatatagcag
      601 atttaatgct ggagagttgc cgattgcgat ggtttctact gataatttct cacagaatgg
      661 tcaacgtttt caagatagta tttttacgat tgcagaagct tggcaaaaag aaggatatgt
      721 ttctgaaaaa tttattgctt atttaaagga taaagaaaaa gtgacttttc cttggagtat
      781 gattgatcga attacgccaa atccttcaga gaatgtaaag gaagatctat tgatgatggg
      841 gctcgaggat ttagatatca ttcacacaaa aaaacatacc aatattgctg ctttcactaa
      901 tactgaggta gttcattatt tagttattga agatagtttt ccaaatggcc ggcctaaatt
      961 ggaaaaggct ggtgtgatta tgacagatag agagacggtt gataaagcag acatcatgaa
     1021 ggttacgaca agcttgaatc cacttcatac tgctttggca gtaacaggtt gtctgctagg
     1081 atatcaatca atttcagcag agatgcagga tgatgatttg gttggtttaa tcaaaggaat
     1141 aggttattta gaaggtttac cagtagttga aaatccaggc attattgatc caaaacaatt
     1201 tattgatgaa gtaattaata aacgtttgcc taatccctat attccagata caccacaacg
     1261 aattgcaaca gatacttctc aaaaagtagc gattcgctat ggtgaaacaa ttaaaaagta
     1321 tgtcgaaaaa gacggaaatg cagaaaatct aacatttatt cctatcgcta ttgctgcttg
     1381 gttacgttat ttactggcag tcgatgatca aggggaaaat tttgtgccaa gtcctgatcc
     1441 gttattggta gagttgcagg ataaattatc agtattgaaa ttaggtgaac aagatccttc
     1501 aaagattcat tgtgcggtac aggaaatttt agaaaataaa gaaatctttg gtttagattt
     1561 atatcaagtt ggggtaggaa agaaaactga aaaaatcttt tgcgaaatgc tagtaggtcc
     1621 tcaagcagtt cgtaaaacaa tcaataaata tataacagaa gttggaggaa agtaactatg
     1681 gaaatgactt ttagatggta tggggcaaat gaagaaaaaa tctcactaca agaaattcgt
     1741 cagatcccag caatgaaagg aattgttgga acattaatgg atataccagc cggagaggtt
     1801 tggccgagag aacgaattaa atctttaaaa gatgaagtcg aagccaatgg acttaccttg
     1861 aaagttattg agagtgttaa tgttcatgat gatatcaaaa ttggtttacc aacacgagat
     1921 caatacattg agaattataa acagacgatt cgcaatttgg cagaatttgg gattgaggtt
     1981 atctgttata attttatgcc agtatttgat tgggtaaaat cggatttgga ttatcgttta
     2041 gatgatgggt cttctacgtt agcctatatt tcaaaaaata tccctactga tccggaggaa
     2101 attattagag aagtagaaaa ttctactaat aattttgaat tgccgggttg ggaacctcat
     2161 cggttatcac aaattaagca attattagaa gcctataaag aagttgatga agagaaatta
     2221 cgagagaact ttgcatattt tctcaaagca attatcccga catgtgaaga agttgggatt
     2281 aaaatggcag ttcacccaga tgatccacca tattccattt tcggattacc tcgtattgtt
     2341 aaaaatcgtg acgatttaga ttggttgtgt aatgtagtgg attctccttc taatggtatt
     2401 acattatgta ctggaagtat tgcagaacga ccagataatg atgtctatga aattctatct
     2461 gaattttgtc aacgggatcg gattcctttt gcacatgttc gtaatatcaa atatttaagt
     2521 gacaatggac tcgattttta cgaagcgccg cataaatcag aatatggttc tctagatatg
     2581 tataagatat taaaaacaat gtacgataat ggatttaatg gctatattcg tccagatcat
     2641 ggtcggatga tttggggaga aaccggtaga cctggttatg gattatatga ccgagctcta
     2701 ggtgcagttt atttaaatgg tatttgggaa gcgcttgaaa agggggcgag taaataatta
     2761 tataagggag tttatttaga ggaaaaattt tctcttctaa aaactaacgg aaggaaagga
     2821 gtgtttaaat atgtttttag ataagaactt tttattagag aatgaaatag ctaaaagact
     2881 ttttcataaa tttgcaaaac accaaccaat tatcgactat cattgtcatt tagacccagc
     2941 atatttgcta gaaaataagc aatacaagaa tttgactcag gtatggttaa atgataaagg
     3001 agctggagat cattataaat ggcgattgat gcgagctaat ggtatagaag aagagttgat
     3061 cagtggtcca ggtgatgatt atgaaaaatt tttagctttt gtaaaaacag tggagaaggc
     3121 gccaggtaat cctatttttg aatggtctca tttggaatta caacgttact ttgatattga
     3181 tttgacaatt tctgagaaga atgcaccaga aatttggcga cgtgctaacg aaaaaattca
     3241 aacggattcc tatcgcccga aagaattgtt ggagatgatg gatgtgaaag ccatctgtac
     3301 aacggatgat cctacagatg acttgtggta tcatactgag ctttctaaac aggataattc
     3361 tttcaaggta ttacctactt tccgtccaga taaggtctgg agtatcaaat catcaggctt
     3421 ttcgaactat attttaaaac ttagtcaaac agcggctata ccaatcaaag actttaatgg
     3481 gttgaagcaa gcattgagcc aacgagtgga tcattttcat caaattggct gtcgattagc
     3541 agatcacggt ttgaatactt atttttatga aatagcagat gaaaaaatac tcgacaatct
     3601 gttaataaaa gctttagcag gaaaaacgga tttccattta tcagaaattg cacaatttgt
     3661 tacaggtatt caattacatt tgatgtctga gtatacaaga tatggttgga cgatgcaaat
     3721 gcatttaaat gctttaagaa ataattcaag tatattaaag gaacaaattg gagtagatgt
     3781 cggtggtgat tcgatggggg atcaagctag tttgacacaa aatgttgtag cattgttcga
     3841 taccgctgaa aagagtgggt gcttaccaaa aacaatttta tattcattaa atccaactga
     3901 ttggcttcca ctagcaacag caatgcaaag cttccaaggg ggtatgattc aaaaaatcca
     3961 attgggatgt gcttggtggt tcaatgatac agctgaagga atgcgtcaac agttgactac
     4021 gatggcacaa caaagtttgt tgtcgaattt tacaggtatg ttgactgatt ctcgaagttt
     4081 tctaagctat cctcgccatg agtattttcg tcgagtatta tgtcaattac taagcgagtg
     4141 ggttaaagca ggccgaattc cagatgatga aatgtatctt agtcagatag tggagaatat
     4201 ttgttatcgt aatgcgtata attattttaa tttttttgaa atcggaagta gaaaataata
     4261 cttgaaataa aagcttaaat ttattctttg taagttagta cagctagaca aagtatgtat
     4321 ccataaagga aggagataga tatgaaaaaa gtagaaattt taagtaagtt agaagcggct
     4381 ggagtcattg cggttgtacg tgggaaaaat aaggatgagg ccattaaagc aataaattca
     4441 attattgcgg gtggaattaa agggattgaa ctaactttta cagttcctaa tgctgaagaa
     4501 ataatcaaag aaattgctaa aaaatatgct gatcaaaaag atgtaataat cggagccggt
     4561 acggttttag atgcgtttac tgctcgtatc gcaattatgg ctggggcaga atatatcgtt
     4621 agtccatctt ttgatcgaca aacagctgag atttgtaatt tatatcaaat tccttattta
     4681 ccaggttgta tgacaattac tgagataaag gaggcactta ctgctggcgt agatatcatt
     4741 aaactattcc caggttctgc ttttggtcca tccattgtaa aagcctatca agctccatta
     4801 ccacacgtaa atataatgcc tactggcgga gtaagtatag agaacttaga agattggttt
     4861 gaagtaggag ttgttgcagt tggtgtaggt gggaatttat tggcttctgt tgaaaaaggt
     4921 gattttgaag aagtaagtcg agtagctagt aaatatatag ataagtttta tgagataaag
     4981 aattattagg agaaaaaaca aataatactc tgaaatgtaa aaaataaata ctgactcttt
     5041 acgcttcaga gtatagtacg tactttcatc agaatgaaaa cgcttaacaa cagaagggag
     5101 agaattgtat ggaaagaaaa tttggtataa gagacaaaat aggatatatg tttggagatt
     5161 ttggaaacga ttttactttc atttttgcta gttctttttt aatgatcttt tatacaaaag
     5221 tattggggat aagcggagct gccgttggaa cattattttt agtagcaaga tttatcgatg
     5281 cagttactga tgtaactatg gggcgtattg ttgatagatc gaaagcaact aagcagggta
     5341 agtttaaacc gtggatttta agaatgagtg gtccagtcgc tttagcaagt tttttaatgt
     5401 atcaaacttc tatgatttca gcttctatga gcgttaaggt agtatacatg tatgttacgt
     5461 atatattatg ggggagtatt ttttacacat ctataaatat tccttatgga tcaatggcat
     5521 cagctattac ttctattaca gaagaaagaa caatgctatc gacattccga aatgttggag
     5581 ctactttagc aagtttggtc attggtgtat taacaccatt atttatttat acaagagatg
     5641 attcaggcgc tcaaat
//
LOCUS       DANKKG010000146         5650 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000146 DANKKG010000000
VERSION     DANKKG010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.146"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            37..426
                     /locus_tag="PW004_002458"
     CDS             37..426
                     /locus_tag="PW004_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HDL2727390.1"
                     /translation="MKEKENYPVQHATKKRWTTVRYGNSDHYIHYDEILYLMSMPGYK
                     NKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENITSISRVNEIVTFAN
                     GEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            602..1345
                     /locus_tag="PW004_002459"
     CDS             602..1345
                     /locus_tag="PW004_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2727391.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            1338..2918
                     /locus_tag="PW004_002460"
     CDS             1338..2918
                     /locus_tag="PW004_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HDL2727392.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(3169..3648)
                     /locus_tag="PW004_002461"
     CDS             complement(3169..3648)
                     /locus_tag="PW004_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDL2727393.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(3664..4416)
                     /locus_tag="PW004_002462"
     CDS             complement(3664..4416)
                     /locus_tag="PW004_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDL2727394.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            4533..4603
                     /locus_tag="PW004_002463"
     tRNA            4533..4603
                     /locus_tag="PW004_002463"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4565..4567,aa:Gly,seq:ccc)
     gene            4964..>5650
                     /locus_tag="PW004_002464"
     CDS             4964..>5650
                     /locus_tag="PW004_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HDL2727395.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTI"
BASE COUNT         1849 a          984 c         1074 g         1743 t
ORIGIN      
        1 tatgaaaaaa ataaaaaaag catccaaagt tacttattga aagaaaaaga aaactatcct
       61 gttcaacatg ctacgaaaaa aaggtggaca actgtccgtt atggtaatag cgatcactat
      121 atccattacg atgaaatcct ttatctaatg agcatgccag gttataaaaa taaaataggc
      181 ctagtattga aagatgaaga gattctcgta aacggcacaa ttaaaaaata taaaaaatca
      241 ctcgaatatg agtatttatg taaagattta aaagcttata tcattaatac tgaaaacatt
      301 acatctatta gcagagtaaa cgaaatagta acatttgcaa atggagaaca actagagatg
      361 ggaaagagaa gtattgataa actaaagaat tttatgcaag aaaagataga aaatgaatca
      421 ttttaaagaa aacaaagact catatcttac ctgttagaag agtgttttcc tgtagaagag
      481 aacaacccaa gtagcagcct agtcgtttct atcctttttt gttatgcact ctttttgaca
      541 tttactccac caataacgaa gggtacaata aaaagtaata acaaacaaaa ggagtacaga
      601 aatgattgaa ttcatcaatg taagtaaaag ttacgggaaa aaacaagcat taaaagactt
      661 gtctctctcg ataagacaag gggaaatttt tggattttta ggacataatg gtgccggaaa
      721 atcaactacg attaaaagtt tagttagtat tattgaacca tcatcgggaa cgattttagt
      781 agatggaatg aaattgacgg aaaatcgatt gagtatcaaa caaaaaatcg gttatgtgcc
      841 tgattcccca gatatttttt tacaattgac ggctggagaa tattgggact tgatttcagc
      901 tgcctatgaa ttagacacac aaaaaaaaga aaagcgttta gcagaattga cagcgctttt
      961 tgatatgtat agtcatcaaa atgaaacaat cgcaagtttt tcacatggaa tgcgtcaaaa
     1021 aacgattttg atcggcacgt tgctgccaga tccagatatt tgggtattgg atgaaccttt
     1081 gcaaggactt gatcctcaag cagcatttga tttgaaagaa atgatgaagg ctcatgccgc
     1141 aaaaggaaaa acagtcattt tttctacaca tgtattggat accgcacaac agttatgcga
     1201 taaactggct attttgaaga aaggtgaatt gatttatcag ggggcagtta gtgaattatt
     1261 gaatggctca cctgatgaaa cgctagaaaa gatttatttg aaaatggctg gaaggcaagc
     1321 gacaggagat ggaccgtatg tataagcgac aattattaga attaattcga gttaatcttt
     1381 tatatgttaa tccacagctc acgaataaag caagagagaa aggaaaaaag ggaaagcgat
     1441 taactatcta tcttttgtct caaaatcttt tttcagggct gattttttta ctgatttacg
     1501 gatttgctat gtttgctatg aactttgtta aattgccagg tttctttact tattacgtgg
     1561 cactttttgg tatgttaggt ttaactcaaa gtatatcagt catttataat atcttttttg
     1621 aaggaaatga cctgcaagta tttttgcctc tgccttttgg acaagaacag gttttttcag
     1681 caaagatttt ggtagttgct ttgaccatca ttccttttgt ttttccgctt ctagtcatgt
     1741 ttattttaac cggatggcga gctgatctag ggattttgct tactatcatt atctcaatcc
     1801 ttttgttttc tctatttttg attattcttt tttgtctggc tagtttgatt gtttttggtt
     1861 tggctcgaac agcagtgttt aaaaaacata gaaaaacagt ttctagtatc ttgcttggca
     1921 gctcgatgct gatcgcattt gctggaatca tgtggatgaa tcatcaaacg gggactattg
     1981 aaaatgagtt aacagatcgg catccgattt cgatattatt gccattctat gatgctgcca
     2041 gtcagccact ttctcaacaa ggtttattgg gttggggaat actagctatc acggctctgg
     2101 gatttttcct tgctgtaaaa cagctgatag taccaaagct tacagagcaa ctgactgatt
     2161 caaacagtga gttcaaaata aaaagaaccc ataaaaagaa ccaaaatgtg catcagcttt
     2221 tatttagtta caatgctcaa ttagtaaaag aacctaattt gattatgcaa gttctttctt
     2281 cttccttgtt aactccaatc atttttattc ttgcttttgc cctaggaggc gaaataaaat
     2341 taactgattt agataataag ttaatcggtg ttgttttcct ggtagggatc gcattggctt
     2401 tcttaacagt taatcagact tcttttatca gcaatcttat ttctttggat caagaaaatt
     2461 ttctgtttat ccgttctttg ccaatctcta tgaaccaata tttaaaagaa aagttcagat
     2521 tcggatggat attgcagagt tttttaactg gtgggatcgc attattaagc ggactgtctt
     2581 ttcagctgcc tttgtttttt atccctagct tgctatttgg ttctttattc ggctgctact
     2641 tattgtcact acgttatttc gctagagatt atcggttgct gctgctgaac tggaccaata
     2701 ttaaccaatt atttaacaga ggttcaggaa gtttgggttt agtcgcaact atgatgggtt
     2761 cattgatcat cagtattatt cttatcgtct tatacggaat gttggctttg ttttatccat
     2821 tttggttagt gaatattcct gtgatcggtt tagttctaat actttccgct ctatggatcg
     2881 tttattatca acgaacgttt tggaaaaaat ttgaataaag aagaggggcc gtgacaaaaa
     2941 tcatgtcgca gccccttttt tacagataga gggtgttcaa aagtcagttt ctcagtatta
     3001 agtcaatttt atgtaaaaat gtgagaagct atttttacaa aacttttttt aatactcata
     3061 attacttctt attgctcctg cggttacttg tcgcaatgtt tgtgagatat tttgtcatcg
     3121 ctcaaggctg aacgtctctt tcacaacctt ttctttgtta cacttcgttt atttaaaaag
     3181 ccgttttttt cgtttcatca tgattgcgtc acgctttact ttgaaattac ggatgttatg
     3241 gttaaagact tctgataata caagacctaa agcgattgct ccagcaatca taattgcttg
     3301 gaccgcataa tgagccgctt ctcgatagga ttcagtaacg agatttcgaa ccgcttgata
     3361 agctaaacca ccagggacta gaggaaccat cccgggaata ttgaaaatcg ttacgggcat
     3421 tttcaaacgt ttagagaaaa gatcgctgat gaatgctact cctaaagaac caagcaatga
     3481 accaaaagca gcagatccgc ctatttgaac acagatccag tagatcatcc agccaaaagc
     3541 accagaaaga ccacaacaaa tcaatgaacg cctaggtaca tttgtaataa cggcaaatgc
     3601 cgcagtcgct aaaaaactaa aagaaaactg aataaatatc gtccccatac ttgtgttcct
     3661 ttcctaataa aaaagctgga aaataaaagc aatcgcaaaa ccgatcatcg tagcagtcat
     3721 catcgcttct gttccgcgag aaactcctga gacataatgt ccagctagca aatcacggat
     3781 agcgttcgta atttgaactc caggaactaa aggcatcaca catccgataa tgattagatc
     3841 ctgattgctt cctaagccaa aacgtacgct gtagatagcg gctatgccaa tcaataaaga
     3901 agataaaaat tccgataaaa atttgattct aaaaaatttc aaactatata gataagctgc
     3961 gtatcccaat cctccaatga ctaaagtcac aggtaaatcg gtcatctttc cgccaaataa
     4021 caccataatt gttccactga tcactgccgc gctaacgatt tgcaaccaga caggaaagaa
     4081 agaacggtct cggtcgattg cttgcagttc gcagtaaagt tcttctaatg ttagctctga
     4141 agcaacatat ttacgtgaaa gatcatttac ttttactact ttttctaaat taatcgaacg
     4201 gttggtaatt tgttccattc ggatggtcga ggtacgatca agaccaataa agagaccggt
     4261 ttgagtgaca tagctgacca aacggaaatt tccagaagct aaagcaattc gactcatcgt
     4321 atcttctacc cgatacatct ccgcattgct ttccatcatg attttcccag cgagtaaaca
     4381 agtgtccagc aacagatcta attcttttcc cgtcatagca tcttctccaa taattgattg
     4441 attagtttta ttttactaca aaacatacca gaagcgtaaa gaataaaaaa acttgaggaa
     4501 ggaataaaag aggcgtatac tgtttttgta ttgcgggtgt agtttagtgg taaaatccca
     4561 gcttcccaag ctgatgtcgc gggttcgatt cccgtcaccc gcttaaaata ggttgtgaaa
     4621 aagctgctct gcaacaagta ataagaacag ccaagcaaaa acaacttctt atgtttttaa
     4681 cttggatgga cttattaccg cagatgcaag cttttgcgca ccgtttacca agcggttgtg
     4741 aaatcagtgt attgtcttga gtatgaagaa aatttttgaa taataataaa taccttacag
     4801 atagagtggt gactgattag agacgaggaa tccgtctctt ttttgtattt ttaagccatc
     4861 ttaaaacagg ttttattttt tgctgtttct atttctttct agtatttatt tataggggaa
     4921 catatgttaa gatttaggga gctgttagta aaggagtaaa gaaatgagca aaaaaacaac
     4981 caaggcgatc gtggggatcg tcgctgctgt gattgcaggg gcattcggtg tttccaccac
     5041 acaaaaagat tcagatatct ttcagcagat tacaactatt tttgattcca caccacaagc
     5101 ttctcaagta gcggatgtgc cagaatatga tggaaaacat caagaaatag aaatcaatgg
     5161 gaatcagccg gaatttacaa aagaggatct tagcctttcc aaaggaacat gggaaagcta
     5221 ttcaaatatt gataaattga acagagtagg acaagccaat gctatgcttg gaaaagattt
     5281 gttccctact gaaaagagag agcctttgta tattgacccg acaggttgga aacaaaagaa
     5341 attaagtgac gggcaatggt tatataatcg cagccattta attggtttcc agctgactgg
     5401 tcaaaataac aacattaaaa acttgatgac agggacacgt tcgttgaatg ctccatacat
     5461 gcttgctcac gaaaatgata ttgcggcata tatcaaagaa acaaatcatc atgtacgcta
     5521 ccgtgtcact cctcattttg aggggaatga actggtagcc aggggagtgc aattggaagc
     5581 agaaagcatt gaagacaaaa aaattgaatt caatgtcttt atctacaatg ttcaagatgg
     5641 ctacacgatc
//
LOCUS       DANKKG010000147         5476 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000147 DANKKG010000000
VERSION     DANKKG010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.147"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            390..1970
                     /locus_tag="PW004_002465"
     CDS             390..1970
                     /locus_tag="PW004_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HDL2727396.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            2168..2392
                     /locus_tag="PW004_002466"
     CDS             2168..2392
                     /locus_tag="PW004_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HDL2727397.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(2597..2917)
                     /locus_tag="PW004_002467"
     CDS             complement(2597..2917)
                     /locus_tag="PW004_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HDL2727398.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(3065..5299)
                     /locus_tag="PW004_002468"
     CDS             complement(3065..5299)
                     /locus_tag="PW004_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2727399.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
BASE COUNT         1661 a         1007 c         1077 g         1731 t
ORIGIN      
        1 gttatgaaag acgtgttgct tgaataaaca agcaacacgt cttttctttt agtcagctga
       61 caaaaaaata tttgactctt atggtttggt ggctttttca ttcgctgaat ccatgctata
      121 ctgattcagt tgtagtagtt gacaaaggtt gtgaaaaagc tatcctgcat taagtaataa
      181 gagcagccaa gcaaaaacag cttttcgtat ttttggcttg ggtgggctta ttgctgatag
      241 gctgtctcta gaacgccgtt tactcggaaa aagagactgc tacacggctt ttgtcacagc
      301 cctatatgaa attgtctttc tttaagagat cggacaatct gtgcctagag aaaatataaa
      361 taattgaatg gaagaaatgg agttaggtta tgaataatcc aaaattaaag caacaagtgg
      421 acaatcgcag aacttttgcg atcatttccc atccggatgc tgggaaaaca acgattacag
      481 aacaattact attgtttggt ggggctatcc gtcaagccgg gaccgtaaaa ggcaaaaaaa
      541 caggtaattt tgccaaatca gactggatgg aaatcgagaa acaacgtggg atttctgtta
      601 ctagttctgt tatgcagttt gattatcaag ggaaacgagt aaatatcttg gatacaccag
      661 gacatgaaga tttttctgaa gatacttatc gtactttgat ggccgtggat agtgcggtca
      721 tggtaatcga tagtgcaaaa gggatcgaag cacagacaaa gaaattattc caagttgtaa
      781 aaaaacgtgg catcccgata tttactttta tcaacaaact agatcgtgat ggtcgtgaac
      841 cattggaatt gttagaagaa ttagaagagc tattagatat cgaatcttat ccgatgaatt
      901 ggccaatcgg tatgggtaaa gggctagaag gtctttatga tatctaccat aatcgtgtag
      961 agttttatcg tccggaaaat tatcaggatg aacgattagt agaattggat gaagatggac
     1021 tgcttcccga aaatcatcct ctaactgaaa attccttata tgaacaagtc ttagaagaag
     1081 tagaactaat caaagaggct ggagatgcat ttaatcaaga aaaaattgct cgtggcgatc
     1141 agacaccagt attttttggt tctgcattga ctaacttcgg tgtacagact tttttagaga
     1201 ctttcgttga attggcacca gctccttatg gacacaaaac acaagatgat cagatggtaa
     1261 gtccttatga ggaagaattt tcaggtttcg tattcaagat ccaagcaaat atgaatccag
     1321 cccatcgtga ccgaatcgct tttgtccgaa tctgttctgg aacattcgaa cgaggaatgg
     1381 atgtctggtt ggaacgtaca aataaaaaac tgaaattaag caatgtcaca caatttatgg
     1441 cagattcgag agaaaacgtc gaaaaagcag tagcaggcga tatcatcggt gtatatgata
     1501 caggcaatta tcagattggg gatacattgt tcgaaggaaa actaaaagtt gcttatgaag
     1561 aactcccttc atttactcca gagctgttta tgaaagtgac tgcaaaaaat gtcatgaaac
     1621 aaaaatcctt ccacaaagga atctatcaat tagttcaaga aggagcgatt cagctttata
     1681 aaacatatct aacggaagaa tatatcattg gagctgttgg tcagctacaa ttcgaagtat
     1741 tccaatatcg tatgttaaat gagtataacg ccgaagtaat catgtctcca atgggacaca
     1801 aaatcgctcg ctggatcgat ccagaagatt tggatgagaa gatgagttca agtcgcaata
     1861 tcttggctcg tgaccgtttc gaccaaccgt tgttcttatt cgagaaccaa tttgcagaac
     1921 gctggtttgc ggataaatat cccaatgtaa aattaaaaag tttgatgtaa agaagtcttg
     1981 acacaagctg ccctgcatca agtaataaga atagccaaac aaaaacagcc tctcatattt
     2041 tgtttagagc cgtgacggat tttccgtgac ggctcttttt actatattaa aatcatagaa
     2101 atgtctaatc aaaaaaatat aaaaattatt ttgcgtacat atgattaaag aaaggagagt
     2161 gtgacacatg gaaacagaat ggcaaagacg tgaaagaaaa aaacagaaaa aagaagagat
     2221 gttacgatta tgtcgattat tagatgtaaa agactgggac aaaaacgagc aaattgcaca
     2281 aaaagtacga tcaatcatcc ataaagagga aaaagttaaa aataaagaca cgcgtagaac
     2341 tgaaaatgaa aagagaaaag tacaaaagaa aagagaaaaa tcaaaaagct agaaagaaaa
     2401 agaggctgag acagaagcgt ttaactccaa gaaataagct ggaattcaca aaaatttgaa
     2461 aagcaatttt cgtgaatttc agcttatttc cgaaggagtt gcttctgctt tcgccgttta
     2521 tacgtgttta gagcgtgaga caaaagtgtt ctttactttt gtcccacgct ctttaattat
     2581 tatgttcaaa atttgtttac acggctaatg tagcgcctaa ttcatgtttt ggaatcgaga
     2641 tttcaacaag gcctgccaat tcgattactt tgatttcatc ctcgtggtaa acggcgtctt
     2701 tgattttatc taagaaataa tttttagcat cttcgatttc tttcggctgg attttacgtt
     2761 tgtcattgat cagcagaatt tcattatgtt caggggcttc agtgtagaca atagtggtat
     2821 ttccggctgt gtagaccttg actagatggg catcagtgtt ttcaagctgg tttaatacaa
     2881 gacgggaatg gctgtttgtt acatttacca atttcatggc gctcacctct tttatgtatt
     2941 tttaattgct tgtttctctt attataattt cttttttata aaatgaacag tattttgact
     3001 tttctgaaaa aagaatgaga cggcatttct caaagaaata ccgtctcacg ctataaaact
     3061 ggatttattc agccgtttct tcggctgatt ctttagccaa acgttctggt ttagaagtaa
     3121 cgatgatttt tccatcttta tccagttttg ctttcaactc atggatagaa gggtgatcaa
     3181 gataaaattc agcgatacca tcttcgattt gttcttggat cgtgcgtcga agaggacgag
     3241 cacccataga tggatcatag cctaagtcaa ctaatttttc tttgacattc gtaggaactt
     3301 cgatgtgaag ttgctgtgct gccagcattt ggttgacatc gtctagcatt agtgtgacga
     3361 ttgtcatcaa gttttctttt gataatgcac tgaattcgat gatcccgtca aaacggttca
     3421 gaaattctgg tgtaaagtag ttgtttaatt ggttcaatac agaacgagta acaccttcgc
     3481 gtgccgcacc aaaaccaacg tttgcttcta ctttaccagt tccggcatta cttgtcataa
     3541 tgatgatcgt atctttaaag ctgaccgttc ttccttgtgc atctgtcaat cgaccatcat
     3601 ctaagatttg caagaacata tgcaaaacat ctggatgagc tttttctact tcatctaaca
     3661 agacaagact gtaaggattt ctccgtactt tttctgttaa ttgaccagct tcttcgtatc
     3721 cgacataacc tggaggagaa ccaatcaatt tagagacact atgtttttcc atatattcag
     3781 acatatcgaa acgaatcatt gaatcttgtg aaccgaatag ttcgtaggca agttgtttcg
     3841 caagctctgt tttacctaca ccagttggtc caacaaataa gaaggagccg attggacgat
     3901 tctgtttgtt taatccaaca cggtttcggc gaatcgcttt cgctactcga tctacagcgt
     3961 tatcttgtcc gatgacatga gctttcaagt catctgctaa gtttttcagt tgtgtctgtt
     4021 ctttttcttt taattcaccc actggaatac ctgtccgttg ttcaacgatt ttttccatat
     4081 ctttttcggt aatcacaggt gtttcctctt cagtgagctg acgttctttc attttttgaa
     4141 gtttattgat ctgatcgcga taataagctg ctttttcgaa atcttcttct cgagaagcta
     4201 aaactttttg ttcttctgct tcttgcagtt ttttctcgat tgttttagga tcaacgattt
     4261 ggatcgtcaa gtttttctta gaacctgttt catctagcaa atcgatggct ttatccggca
     4321 agaagcgatc ttggacgtaa cgattggata aagttgcggc tgcttcgatg gcctcatcag
     4381 tatattttac atgatgatag tcttcataac gtttttgcaa acctttcaaa atactgatcg
     4441 tttcttcgac agttggttca tctacccgta caggttgcat acgacgttct aaagcagcgt
     4501 ctttttcgat gatgcggtac tcatttaagg tagtagcacc aaccatttgc aattctcctc
     4561 gagctaaagc tggttttaaa atattaccag catccatatt tccatcacca gcagaaccag
     4621 caccgacgat ttcgtgaact tcatctatga ataggatcac attttctgct tgacggatct
     4681 cttcaatcaa tttttgcata cgttcttcaa attgtcctcg tataccagta ccttggacta
     4741 aagatacgac atctaaacga atgacttctt tgtccattaa tttttgcggt acatcaccat
     4801 ctacgatttt ttgtgctaaa ccttcaacaa ctgctgtttt accaacacct ggttctccaa
     4861 tcaagacagg attgttcttg gttcggcgat ttaaaatttc aatgactcgt ttgatttcgt
     4921 catcgcgtcc gatcacagga tcgatatcac cttgtcgtgc ttgttctgtg atattgattc
     4981 cgtattctcc caataaacct tctgtattgc cttgaggtgg ctgaccacca ttgaagttgt
     5041 tgtttccgcc aaattgtgtt ggtggtgttt gctcataatt tccttgctgt tgcatttggc
     5101 gagacatgga acggaaaaga tcgtccaaac tgccaaatcc aaatggatca ttttgggcca
     5161 tattagtcag acctccattt tgttgatttt tgattttttg ataacagctc tgacagtaat
     5221 cgagttgtgt ccgctgacca ttgacagtgg catacaagtg aatcgttgcc tcattcttgc
     5281 cgcagttttg acaaagcatg aatcattcac gtcctttcac aaaaatgggt acatccatat
     5341 tttacatctc gtttttcatg aggtaaaaga ataaccccta gttttttaag agatttatga
     5401 ggtagaaaag gtgaaatctc tattgaccat tcctgacctt tgactcttat tatactaaaa
     5461 ataaaaaggg aagcaa
//
LOCUS       DANKKG010000148         5119 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000148 DANKKG010000000
VERSION     DANKKG010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5119
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.148"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(17..769)
                     /locus_tag="PW004_002469"
     CDS             complement(17..769)
                     /locus_tag="PW004_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL2727400.1"
                     /translation="MIIRKPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTIPSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLEPNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            complement(784..2760)
                     /locus_tag="PW004_002470"
     CDS             complement(784..2760)
                     /locus_tag="PW004_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HDL2727401.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYRAYAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PTEDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDKEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLVDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITRLTEGEYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKVSV
                     PMTGSNGFQTYVLISCLLLGAGALSAVVYFKKKA"
     gene            complement(2813..3484)
                     /locus_tag="PW004_002471"
     CDS             complement(2813..3484)
                     /locus_tag="PW004_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HDL2727402.1"
                     /translation="MKKWLFGFLGVALIVFCSVFGYVSYQKHEGDVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTCKFIVSEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            complement(3904..4137)
                     /locus_tag="PW004_002472"
     CDS             complement(3904..4137)
                     /locus_tag="PW004_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727403.1"
                     /translation="MARPVLDAMDMDTLTKGRYVQLLARGYSPKDVFKALSKGTDMEK
                     ERLRKEFDYWREHNGIKDLKPARPVIRRKKLKK"
     gene            complement(4329..4619)
                     /locus_tag="PW004_002473"
     CDS             complement(4329..4619)
                     /locus_tag="PW004_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727404.1"
                     /translation="MKFFEVKSPYYYALIAAKNEEECAELYEQVVTDIENREEFIRCL
                     HELNRNEAIQEDAKKISEETLQPIGMEEATKDIFTIINEQKSCVLSIDSTLV"
     gene            complement(4638..4979)
                     /locus_tag="PW004_002474"
     CDS             complement(4638..4979)
                     /locus_tag="PW004_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HDL2727405.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     NEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
BASE COUNT         1376 a         1082 c          726 g         1935 t
ORIGIN      
        1 tgttggttac cctcctttat tttttctttt tataaacaaa tagcgaacca atgatcatga
       61 ctgaaagaaa aacagtactt cctaaaagcc atttccatga taatttttgt ttcttatttt
      121 cttcatcttt taattctgat tttgtaaagg ttactcgctt cccatacact agaaaacggt
      181 gtgtgttgat ccccttaggc gtacatgtca ttagcgttac tcgatcttca ttgggatttg
      241 gttctaagta agtgacaaag tttctttgta gttcgtcttt ttgcacttcc tcaatccgat
      301 cgatttgata agcgtgctgc tcaccatcaa ctttgatata aaacttttct ccctttttta
      361 ctgaaggtaa atcatcaaaa agattatctt tataaagtcc actatgacct gcgattaaag
      421 gatttttacc gtttcctcct gtaatatcac cagttccttc cgtaatacct acaccatttt
      481 ctaaaatttt gtcggacgtt ccgtcataaa ttggtagttt taaagaaata cttggaattg
      541 taagaatacc aacaggatcg cctaactcaa tcttagcaac atccaaagaa gaatccgttt
      601 ctgctgccgt tcctgttcga tcgattgcgg caatctgctg attttgttgg tatttcgcca
      661 cgctcgcttc agcaggcatc aaaagtaaga cacctacagc tgaaagtaac cagaaagcaa
      721 tgatccattt acccatgatt cgctttttct taggcttcct gataatcatt ggttttgatc
      781 cttctaggct ttcttcttaa agtaaacaac tgcagaaagt gcccctgctc ctagcaacaa
      841 gcaagagatt aatacatacg tttgaaaacc gttgctacca gtcattggta cggaaacttt
      901 ttgattgttt ttgatcgtga ttcggttttc ttctttgtat gaatctttgt ctactttgaa
      961 agagatctct ttcgttaatt tttgatagtt ttcaggcgct tttgtttctt ccaatgaata
     1021 ttcaccttca gttaaacgag tgatttcaaa gtgaccttct ttatctgatt ttaggatcgc
     1081 tgcatttgct acatcgtcta cccatactgc ttctttgaca ccgtctttat ccgtatcaac
     1141 taatccacca taatattttc cttctgcgtt tttagcagca aattctgcgc cttcaagcgg
     1201 tgttgctttg attcctgttc caaatagtcc gctatcgact tttaagaatt ttgcaccata
     1261 agtcacaacg tcagctttcg ttttttcgtg gtgttcttcc tgattaaatg gattgttgtt
     1321 ataaacgaaa tccacgctat ttgttaatgg attggcaaca gctgcgctat catttacata
     1381 catgttataa gtcatttgaa gatcttgtcc gtgtaaactt gctacagtat cacttacttg
     1441 gccattattc acgataaaat cgatcacaaa gccattttct gaattttgga ttttataatc
     1501 tgtattttct tttaatacca cagatccatc tttatttgtt actttgatat ttgctttagc
     1561 agcgacataa gaaagtcctt gtcctgctgt atccccccat ttaaatacac tgtaaggaga
     1621 agtttcgctt ttcactccgt ttactgtgat accgtctgct ccgcctgcaa tgtctgttgg
     1681 gatatgaatc gttccttgat aatttactgc gtcaccaatt tggaaagaat gttcttgtcc
     1741 tgttccattt gttactgttt tatcgatcac tggtgcttta tagtttacgt atgaagcttt
     1801 taagtcactt gtcacgccat cattcgtaat ttcaaaggtt aatttattgt gcgcatcgtt
     1861 gcgagcgtct gcgcctaata aatattgatc tgctgttggt tctttatctg atcccactac
     1921 aacttctgaa ggaacttcca caaaatagta ttttcctaac gtcaaatcag tagcagtcag
     1981 ctttccttga tcatccgata caagatctcc taattttgtt ccttttccag gttcgccctt
     2041 gtataactca aattttgccc cttttaaagg tgtttgtttg ctttgagctg tgccatcgtc
     2101 accaaatttc gttaattcaa aactcaactt agaaacaata tttttaggat ataagtgaat
     2161 gtcttttaaa aaacctgaac ctgtattatc agtcattggc gcaatcacaa ccattggttt
     2221 tgctttttgt gtgactaatc cagcagcgga tttactttca taaaccacat atacattccc
     2281 tttggaattg acataggctg gttgatcagc aaaagtgatc tcaccatttt catctacagc
     2341 tgaagtaatt ttattggttg cattcttgac gtactcactg ttagatccct tgtcttctac
     2401 atctttaacg atttcgtcca ctttcgcgtt ggttagatca gaatcttcag tcggtaatac
     2461 ttgatccgta atatttccta acgtaaactc aacatcacca taatctgcct tgttgtattt
     2521 cgttacacct gtaggatatt gatcgtgcgt ataaccatcg tttttgatcc cgtcaatatc
     2581 aacattcaat tgcgttcctt gatcgtacat taatttatga accgtgatat tgaccttctc
     2641 tggtggcgtt ttttgcgtga catcttctgc atatgctcga tatccctgaa agcagcctgc
     2701 aatgattcct aacattccaa caagcattcc agcttttttt acataccgat ttattgccat
     2761 tttttactct cctcatttca tatttcaacg actcgcgttg attacaacct tgttattgta
     2821 gctctccctg taccacgtat ctttcttttc cttgtgacgt ataaacacaa gtaaacaacg
     2881 ttaataaggg tttctctcct ttctttggta aagacaagaa agagttctct tcaggaacgc
     2941 catcaattaa tttaaagtac tcagatacga taaatttaca cgttaccttg taacgataga
     3001 cgccatcgtt actaactaag tccatcacct cccctaatga caacttttga atatctgaaa
     3061 ataaaacacc tggcatttcc atgttgtgtc ctgctagaac atagtttcct ttgcccattt
     3121 cggcatcctc ataaaaataa gtagccgctc caagactcaa agtgtattga tttgcccctt
     3181 taaaaatcgg caatgctaaa ccaagtgcag gaatctgaat ttgtgcaaat acttgtttat
     3241 tgacgcccat ttcgattgct tctttttgta tggacaacat ttgccccaaa gacatatcat
     3301 tattgacgtt cgttgcatcc tccttataac tctttgcgtg tgcattgatt tgatctacgg
     3361 gcatcttttt ttctatattt tgcttaaaca catctccttc atgtttttgg tagctaacat
     3421 agccaaacac actgcaaaag acgattaaag cgactcctaa aaaaccaaat aaccattttt
     3481 tcatccatct gcctcctttc agacaaaaca aaaaaacttt ttctcatttc catagaaaaa
     3541 gttttaaaaa tgaattctat taatgaatgt catgagactt ctaacaatag ttgacaatta
     3601 gaactttttt tcacataagt tcgtctcaac tgttcaaagt ttttcattga acctaacaaa
     3661 caagctttct cttttcctgt taactgcata tcattgatat attgcgcgac agataatctt
     3721 ttaaggtgtt tttgtttcat tgtttttgtt tcattgtttt tgtttcattg tttttagtaa
     3781 cttttcttcc atttctttcg acactttttt tctattttcg atttctttac aattcatgag
     3841 ttttccttcc tttcattatt tttatcaaaa taaaaaagcc tatgtacgcc atacgctttg
     3901 gtcttacttc ttcaattttt ttcgtctaat aactggacgt gctggtttta aatcttttat
     3961 tccgttatgc tctctccaat agtcgaattc ttttcgtaat ctttcttttt ccatatctgt
     4021 tcctttagat agggctttga atacatcttt aggtgaataa ccacgagcta acagctgtac
     4081 ataccttcct tttgtcaaag tatccatatc catcgcatct aaaactggtc gcgccatcta
     4141 attttcctcc taaataaatt ccttactcaa tgcctaaggt aatactacct ttttattaat
     4201 cgccgtgatt ccttctaatc ctttatctgt aaaatctaat ttgtatggtt attttcctaa
     4261 gtaattggtc tgagaatcat tacctaattt ctttttactt tgaaaaaaat tatagaatct
     4321 tggatctatt aaaccaatgt agaatcaatg cttaatacac aagatttttg ttcattaata
     4381 attgtaaaaa tgtcttttgt agcttcctcc ataccgattg gttgtaacgt ttcttcacta
     4441 atttttttcg catcctcttg aattgcttca tttcgattta actcatgcaa acatcgaata
     4501 aattcttcac gattttcgat atctgttact acttgttcat acaattctgc acattcttct
     4561 tcattctttg ctgcgattaa cgcataatag tatggtgatt ttacttcgaa aaatttcata
     4621 atttattcct ccagttttta ttcttcttta tccgctacaa attcaatgat ttcaacgctg
     4681 acaatataat cactcaaata tgaccaagta tcttcaacag ataattcgtc tccattatca
     4741 ttcataagcg tcattttaaa ccattcatta ccttcatcat cttcaggtag caagccgaat
     4801 ccaattggat tatcagtcaa tgtttgatcg tataaatcat cctcgtttaa tgctaaaaca
     4861 tctaatcctt tacaatttcc gcctcttcta aacgtcacaa aacctttgta atcccaacgt
     4921 tggaaagaaa ccttaatagt atgtgtgccc aatcgtatat tcggatcata atttttcatt
     4981 tgtcattcct ccattacgta aatttcattt tctctatgtt ttatatcaat tttttcactt
     5041 tattcaggac tcgttcatat tttatcgttt ttcgtttact taaaaataca taaaaaatat
     5101 cgactcgttt atggcgtcc
//
LOCUS       DANKKG010000149         5019 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000149 DANKKG010000000
VERSION     DANKKG010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5019
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.149"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            98..667
                     /locus_tag="PW004_002475"
     CDS             98..667
                     /locus_tag="PW004_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2727406.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(845..1285)
                     /locus_tag="PW004_002476"
     CDS             complement(845..1285)
                     /locus_tag="PW004_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDL2727407.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            1443..2207
                     /gene="map"
                     /locus_tag="PW004_002477"
     CDS             1443..2207
                     /gene="map"
                     /locus_tag="PW004_002477"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HDL2727408.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            2239..3162
                     /locus_tag="PW004_002478"
     CDS             2239..3162
                     /locus_tag="PW004_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HDL2727409.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            complement(3387..3626)
                     /locus_tag="PW004_002479"
     CDS             complement(3387..3626)
                     /locus_tag="PW004_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2727410.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(3631..4050)
                     /locus_tag="PW004_002480"
     CDS             complement(3631..4050)
                     /locus_tag="PW004_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL2727411.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            complement(4277..>5019)
                     /locus_tag="PW004_002481"
     CDS             complement(4277..>5019)
                     /locus_tag="PW004_002481"
                     /inference="COORDINATES: protein motif:HMM:NF019310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2727412.1"
                     /translation="INPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLK
                     DSYTFLKKEKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALS
                     LGGLCGGILTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCF
                     VAMGVSTMFVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQE
                     TWVVMFVASIASIMISIYSKRIFQKLEI"
BASE COUNT         1666 a          896 c          888 g         1569 t
ORIGIN      
        1 aaaatcaaaa actaattttg catatcaaaa aaaacatgtt acaatgaaat taatcaatgg
       61 tggaagcgtt ataaaaaaat taaggagatt aaaaaatatg gcaagaaaaa agacgattac
      121 tagagaacag attttggcag ctgcttatga agtagctgcc accgatggat ttacacgatt
      181 tactgcgaga aacatcgcga acaaaatgaa ttgttccacg caaccgattt atcttgagtt
      241 taaaaatatg gatgacttga gacaagctct ttttgagcag atctatgatc atttacaata
      301 tgaagtgttc actgttgagc atacagggaa tacgattatc gatcttgctt ttaattatat
      361 ccattttgca agaagagaga agaagttgta tcgttcactg tacttagaag aatatggtgg
      421 aggaaaagag atgcagaatt tctcttatac ttactttgtg aatacagtga aacgcgatcc
      481 agcttatgtg aatctttcag aagagcagat cgaatctttg tataatggga catggatcat
      541 agctactggg atcgcagctt tgatgtcttc aagcatcatc catccaactg atgaacagat
      601 tgaaaagatg atccaagaca gtatagatgc tatcttgaaa ttcaaagaac caatcgaaat
      661 cgattgaata tactcaaaag ttccacaaat cagtaggtaa caagagatgt gtcaaaataa
      721 ggaaaagttc gtttttctta cagcgtatgc atttttaact cccgaagaac tcttccatga
      781 atgcaatgaa taaaagagcc atacagattg aataaatctg gatggctctt ttattgcaag
      841 tatattaaat ttttgcgact agttctttag cgaaagcttc taaattttcg atatcttctt
      901 cttcagcatt cagatccact ttaacatttt cggctccttt tgatgcgcct gtttttgcaa
      961 aagcagcatc gaaatcatcc accgatttgc agaattcatc gtaaaaagta tcaccagatc
     1021 cgcagacacc gtaaattttt cccaatagat ccaattcttg caagtcttca taaaagtcta
     1081 cgatctcatc aggcagatcg ccatcatcgt atgtataagt agctactacg caaatatctg
     1141 cctcctcgaa ttcatctgca tctacttggg tacattcgtt gatttcgact tcgatatcca
     1201 gattttcaaa tgcttctgca acaatatcgg cgatttcttc agtgtttcct gtcatgctag
     1261 catatacgat ttttgctaaa gccatgatgt gcctcctcgt ttataattat ttaacaatta
     1321 tgaattatac caaaacatca ttgaaaagaa aataaaacct agagacacct ggggaatgtc
     1381 atttggctaa atgtatgccg ctatgttaaa atgagaggga tttgtaaaaa ggagacgaaa
     1441 atatgattac tttaaaatca gcacgtgaaa ttgaaatgat ggcagaatct ggagctttat
     1501 tagcagatgt acataaaaat ctgcgtgatt ttataaaacc aggaatcaca agttgggata
     1561 ttgaggtatt tgtcagaaac tatatcgaga gtcatggcgg aattgctgcg caaattggct
     1621 ttgaagggta tgaatatgcg acttgtatca gtataaatga tgaaatttgt catggattcc
     1681 ctcgtaaaaa gcctttgaaa aacggtgatt tggtaaaagt cgatatgtgt atcgatttga
     1741 aaggcggcat ttctgattct tgttgggcct atgtagttgg agaatctaca cctgagatcg
     1801 accgattgat ggaagtaacg aagaaagctt tgtacatcgg tattgagcaa gcacaagtag
     1861 ggaatcggat tggtgacatc ggtcatgcta tccaaactta tgtagaaggc gaaaatctag
     1921 cgatcgttcg tgattttatt ggtcatggcg ttggtcctac gattcatgaa gaaccagcag
     1981 ttcctcatta cggagaagct ggaaaagggt tgcggttgaa agaaggcatg gttatcacga
     2041 tcgaacctat ggttaatacc ggaacttgga aaatgaaaat ggatcctaac ggctggactg
     2101 cgtatacaag agacggcgga ctttcttgcc aatatgaaca tacattggct attactaaag
     2161 atggtccaag aattttgaca tcacaaggag aagaaggcac ttattgatct atccagtcaa
     2221 taggtacggg agaggtacat gaaagtttgg aacaaaataa aacaaaatga aaatctgatg
     2281 cgctttattg agacgactca aaagcgcatc actgattcag aaatgagtac gacctctgtc
     2341 gtcgttgctt attatttact cttgtcttta tttccattga ttattgcttt tggtaatatt
     2401 ttaccgtttc tccacatcga tcaagagact gtactgacct atttacgaga agtgattcct
     2461 gagaccgttt accagtttat cggtcctgca atcaaagacc ttttgacaca aagttcagga
     2521 ggcttgttat ccgtttctgc attagcggct ttatggtctg caagtcaaag tatcaatgct
     2581 ttgcaaattg cgatgaataa agcgtatggc gtagaaaata gaaaaaattt tttgatcgtc
     2641 cgtttcttct cactaattgt cattgtcttg ttcatgattg ctattagtgg ggtaactatc
     2701 gttcttgggt tgggacagct gattttggat gctattcagc caatcgtaca aatacccgtg
     2761 aactttatcg atcagttcca aactttgaag tggccaatca cattggtagc tttatttgtc
     2821 atcatgttct tgatttattt aattgtccct aatgctcagt tgaagttgaa gacgatcatt
     2881 ccgggcacga tcttcgctac tgtcggctgg atgctgttat cacaagtgtt tagtatctat
     2941 gctaggtatt ttgcaacgag gatcagcggt tatcagatca taggaagttt tattgtactc
     3001 atgctttggc ttaattttgc tgctacgatc atcattttgg gcggaatcat caatgcagtt
     3061 gttcaagaat acgtgacggg tcatcaaatc aaaaaacgtc gagatccaag ccgaaaatgg
     3121 ttcaataaat tgaaaactaa attttctcat aaaaaaaagt agaaacaaga gttcttattg
     3181 taaatttata tcagtaattt atttgaaatt ttatatctaa ttttcaatta tccgcataaa
     3241 cactatacat tcaagcattt tgaagtatag aaaaacactc atcttaccac gaatttacca
     3301 cgattgaatg agccttgata tgaccgtaaa actactaagg gagatagcac gaaactgtgt
     3361 atctcccgat ttttttatac agttgattac ttcactccga tttcaaattt caatattgct
     3421 ttcatcatgt ctgtatgaat ttctcctttt agttcttcat caacctgcat aatgatttta
     3481 ccactttcag agtggtaaaa aggacgcaag cataatttag aaatataacc gtcataaaac
     3541 ttcaaaatct ctttaactgc catttcatct ccgcttgacg ctttgcaaat caattcaaaa
     3601 gagggatagc cataatcttt actcatttct ttacttcctt tcgctcaaaa gcatttttaa
     3661 cttccttagt cctctagttc ttctactgca aatagcagaa cgtgatacat ggtacagttc
     3721 tgaaatttct ctgtcgttca ttccttgaaa ataacgtaat aaaatcgcac tacgctgttt
     3781 ttctgacagt ctatgtaaaa ctgtaccaag tgtttcatca gatacataaa ttgtaaagtg
     3841 caagacttta aatgatacac aatcataaaa acaggtatca atcgttccac gttcaacttg
     3901 tttcgtttcg gctatttcac aaaacaatac ttcacgttta gaatgtcttg caagctgtct
     3961 gtttcgactt ttcactttgt tcttaatgac aatcagcatt aaagaattga actgtaatct
     4021 gacgatatgc tcgaaagaag atgtcctcat aatctcaccc cctttctgct aatgctgaaa
     4081 tatataaacg gaataccgtt tatacccttt agaaatacag aaaaatggta agaaaaaagc
     4141 ccacacaaaa catatatttg tgcgagcctt aaatactgca atattactct ttcttttata
     4201 acactatcct tttagcagat ttcaaatttt cttttatata ttgtttttag cctaacatac
     4261 tatttaaccc cttgtattat atctctaact tttgaaaaat tctttttgaa tatattgaaa
     4321 tcatgattga agcaatagaa gctacaaaca taacaaccca tgtttcttgt ataaatacat
     4381 cgaataaaat accgtaaacc atttgtccaa taggttgacc gcacatagca actgctacca
     4441 gcgtagccat tatctttcct actaattgcg gtggtgtttg tgtctgaaca agagtgtaaa
     4501 tttgaacaac aaacatagta gatacaccca tagccacaaa gcacatcact gtaactaccc
     4561 agtaggaaac tattgcaggc aattctaaaa atagcgaaat tcccataagt aaaacagaaa
     4621 cagaacaaat aagtaacaag gtataaaggt tttttagttt taactttgaa gcaacaagag
     4681 ctgttaaaat accaccacat aaaccaccta atgataatgc tgattgtgaa aaaccatata
     4741 gtgtatctgg catttttaaa acttcagaaa ttaagatagg cagaccaaca attaaaacag
     4801 cacttaaaac caaattgaaa atgcaaacca aaaagattac ggatataaaa atgggtttct
     4861 cctttttcaa aaaggtataa ctatctttta aatcgttttt agcaactgca attacaccta
     4921 cttcacgagg tcttttttgg tgtggaatat ggataaaaat ttccataatg gctgaaaaag
     4981 taaaacatat aatactaaca attaaaatgg gattgatac
//
LOCUS       DANKKG010000150         4914 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000150 DANKKG010000000
VERSION     DANKKG010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4914
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.150"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            39..524
                     /locus_tag="PW004_002482"
     CDS             39..524
                     /locus_tag="PW004_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HDL2727413.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(628..1221)
                     /gene="clpP"
                     /locus_tag="PW004_002483"
     CDS             complement(628..1221)
                     /gene="clpP"
                     /locus_tag="PW004_002483"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDL2727414.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            1401..1928
                     /locus_tag="PW004_002484"
     CDS             1401..1928
                     /locus_tag="PW004_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727415.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            2019..2756
                     /locus_tag="PW004_002485"
     CDS             2019..2756
                     /locus_tag="PW004_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDL2727416.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            2760..3677
                     /gene="miaA"
                     /locus_tag="PW004_002486"
     CDS             2760..3677
                     /gene="miaA"
                     /locus_tag="PW004_002486"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HDL2727417.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            3679..4908
                     /gene="hflX"
                     /locus_tag="PW004_002487"
     CDS             3679..4908
                     /gene="hflX"
                     /locus_tag="PW004_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDL2727418.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         1672 a          845 c         1107 g         1290 t
ORIGIN      
        1 gattagtttc tacaaaaaat gaaaggagaa aaataaatat ggaacggttt atagcatcaa
       61 atgccacagt gattggtgat gtgacattgt cagaagatgt gacgatctgg tatcaagctg
      121 ttttacgcgg ggacagtaat tggataaaaa taggacaaag aacgaatatc caagatggta
      181 caatcatcca tgtcgatcat gatgcacctg tagatattgc ggagaatgtg acggtaggac
      241 atcagtgcat gctacatggc tgtacaatcg aaaaaggggc gctgatcggg atggggacca
      301 cgatattgaa tcatgcggtt attggtgaga atagtttgat tggtgctgga tcattagtaa
      361 cagaaggaaa agttattcct cctaatgtct tggcttttgg tcgcccagct aaagtgatcc
      421 gtcctttgac aaaagaagaa atacaaaaaa acaaagaaaa catccagcat tatgtcgaga
      481 taggtcggat gcatgcgcaa ggaaaattca aggagcaaag ataggcagta gcatccataa
      541 aataaaaaag gatggtcagt ttgagttgac catccttttt tatttggttt agataagaga
      601 gcttaaaaaa gcttcctttg aaacagatta atttaatgaa gaactgtttt ccatgacttc
      661 atcgatcaat ccatatgctt ttgcttgttc agctgtcata tagttgtcac gatctgtatc
      721 tttttcaatc acttcaagcg gttgtccagt gcgttcagct aaaattttgt ttaagcgttc
      781 gcgtgtttgc aaaatatggc gtgccgcaat ttcgatttca gttgcttgcc cttgagcgcc
      841 gccaagaggt tgatggatca tgatctcagc atttggtaaa gcaaatcgtt taccttttgt
      901 accagctgtc agcaagaagc ttcccattga agcagccata cccataacaa ttgtttggac
      961 atctgctttg acaaagttca ttgtgtcgta aatagccata cctgctgtaa ctgaaccacc
     1021 aggagaattg atataaagat agatatcttt ttcagaatct tgagcgtcta agaaaagcaa
     1081 ttgggcgatg atcgaattag caagatcatc tgttacttgg ccgcttaaca tgatgatacg
     1141 gtctttcaat aaacgtgagt agatatcgta agctctttca cctcgtgaag attgttcaat
     1201 gactgtagga attaaattca taatagagtt cctccttgat ttcgtttaga aatagtgtac
     1261 cataatttag tttctaatac ctaaaatgta gtatacattt ttggtcaaaa aaggtcaaat
     1321 aaaaaactta ggcaaattag tcgcataaac ccgctttatg gtatgataag cagagaaaca
     1381 aaacaaggag gaacaacaga ttgatagttg gtattactgg ccttatcgga gtgctgacag
     1441 gtgctgtagt tgtctatatc ggcatgatac tgcaaatgcg tcacttggaa aataaagaac
     1501 agcgtagacg tgaattggaa ttgaaagtca aagaaatcga gacgctgaat caattaaata
     1561 aaaagatcaa cgagatcttg caaaaacgga caacgctgat ggaagaatat gtcagctttg
     1621 atgcatttga tgattgttat atcacgattg acgattttgc gtatctccaa tcattttcag
     1681 cgcaaaatag tttttatctg ccaaattata ttttagacga gctatttaag aatatctccc
     1741 atcgaaaagt gattttatct ccagatgaaa caatgaagat tggtggatat gcttataaag
     1801 gcggacgcgt gattttagag aattttacgg atagtctgac tgaaatgatc aatgaaaaaa
     1861 aagctcagtt gaagactatg acgcgccagc cgattggttt cttctcgaaa gatgatgttt
     1921 ggagctgata ttttgaagca ttgccgttgc acatgagagc gtggtaatgc tttctttttt
     1981 tcttaatgat acaatagaac tgaacggagt gaagaaagat gttacaaatc tatgcccatc
     2041 gcggaagcag cggcacccac ccagaaaata cgcttcctgc atttgcagaa gctgtacgag
     2101 taggtgcaga tggtatcgaa ctagatgtcc atctaagcaa agatggttat ttgatcgtta
     2161 tgcacgacga agaagtggac cggacgacta acggtaaagg gctgattcga gaaaaaacat
     2221 tggaagaact caaaaaattg aatgcagggg gcaaattcag caaagaattt ccagcagcta
     2281 aagttcctga attgagagaa gtgttaggac tgctcctcca aaaaaattac cgagggatgc
     2341 taatgattga aattaaaact gatcagtatg aatatcccgg tatcgaagaa aaattagccg
     2401 aaacattaaa aagtagagat tggccttttc aacatgcata ttgcagcttt catttggatt
     2461 ctttggaaaa aatcgcatta ctggaaccgg agatacagct ggacttattg atgagtactt
     2521 ctgataaaaa gatccaacta gcagcagaaa ccccttatat agaaggagta catccaaaaa
     2581 atacttggtt gttcgaacat ccagtccagg cagctgattt tccaaaagca attcgtgcat
     2641 ggacgattaa cgatgaagaa gaaatggcca aatgtcaaga actcaaaata gatggaataa
     2701 taactgattt tccggagaaa gctcttcggt ggaaacaatc aacgaaaagt aggtaaaata
     2761 tggaaaaagt attagtgatc gtcggtccga cagcagtcgg taaaacagcg ctaagtgtgg
     2821 aactcgcaca actatttgga ggagagatca tcagtggtga ttcattgcaa atctataaaa
     2881 atctggatat cggtacagca aaaatcaaag aagaagagat gaataatgtt ccgcatcatt
     2941 tgattgatgt gatcgaacct actcaacgtt actcagccgc tgattttcag cattcaggtc
     3001 gacaattgat taccgagatt gcgaaccgag ggcacctacc tatcatagct ggtggtacgg
     3061 gtctctatat ccaatccctt ctatatgatt atcagttagg agcgacagaa gaagcgaatc
     3121 ctgagattag aaaaaagtat gaaacgttag ctgaaacaat tggttcccaa gcattatgga
     3181 atcagttgaa agaaaaagac ccggcagctg ctgagaaaat ccactggaac aatcaacgca
     3241 aagtgataag agctctggaa gttttagaga cgacaggaca tagtatcacg gcacctaaag
     3301 aagagccaaa gcaattgtat gattacctca tgattggatt agatacgaag cgtagtattc
     3361 tttatgaacg aatcaatcaa agagtcgatt tgatgctagc agaaggttta gtagcagaag
     3421 cgcgtaaggt gtatgagctt ggagaagtcc aggccagtca aggtattgga tataaagaat
     3481 ttttccctta ttttgaaggg aaagaaacat tggaagaagc aacagaacaa gtcaaactac
     3541 attcgagaag atacgcgaaa cgtcagttga cgtggtttcg taaccgtttg gatgcacagt
     3601 ggtttgatct tataagtgag ccaaatcaaa aaagtcagat tgagcaaaca atcaaaaaat
     3661 ggctggaggc ggaataaaat ggaagaaaaa gtgatcatcg taggggtaga aaccgaagca
     3721 aaccagcgtt acttttcaga gtcaatggaa gagctagttc agctgacgaa taccgcatca
     3781 ggagaagtcg tattcacaat tacacaaaag agaccacaag tggatcggca aacgattatc
     3841 ggtaaaggga aattacaaga attagttcaa caagcggatg cgcatgaagc ggatttgatc
     3901 atctttaatc atgagctaac tccgagacag agccaactga ttaccgatgc tgttggtctt
     3961 ccggtaattg atcgcgtgca gcttatctta gatatttttg cgatgcgtgc tcgttcgaaa
     4021 gaagggaagc tgcaggttga acttgctcag ttagaatacc tgctgcctcg tttagtaggg
     4081 caagggaaaa cattatctcg tcttggcggt gggatcggaa caagagggcc aggtgaaaca
     4141 aagttagaaa cagaccgccg acatatacgg aataaaattt ttgctgttaa gaaagaattg
     4201 aaagaagtag aagctcatcg agagcgcaat cgccaaaaaa gaaaaaatag cgagattttt
     4261 cagattggac ttattggcta cacgaatgcc gggaagtcaa ctatcttgaa tctattgaca
     4321 caagcggata cttattcgaa agatcaattg tttgcaacgt tagatccatt gactaagaga
     4381 tggcgttttg ctgaaggatt tgagattact gtaacggata ccgtcgggtt catccaagac
     4441 ctgcctacgc aactgatcga tgctttccat tctacattag aagaaagtca aaatatggat
     4501 cttttgctac atgtagtcga tgctagttct ccagatcgta ttttacagga gcagactgtc
     4561 ttaaaattga tggatgaact gaatatgaaa gaaatgccga tactgactgt ttacaataag
     4621 gcagaccaga tcgacccagc gatgttcacg cctacgctat tcccaaatgt attgatttct
     4681 actcagacga aagaaggtaa gatggcgttg atcgaagcaa tcaaacgtca gctgatggaa
     4741 ctaatgaccc cttatacact ttctgtgcca agcaacgaag gacagaaatt aagcgagttg
     4801 agaagacaaa caatggtttt ggacgaagcg tacatggaag aaacaaacga atatgaagtt
     4861 aaagggtttg caaaaaccaa ttcaaaatgg ttaagaaaaa accaataaaa tttt
//
LOCUS       DANKKG010000151         4885 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000151 DANKKG010000000
VERSION     DANKKG010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4885
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.151"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            512..2356
                     /locus_tag="PW004_002488"
     CDS             512..2356
                     /locus_tag="PW004_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2727419.1"
                     /translation="MNYKQTAEEVLQYIGGEENISHLEHCSTRLRFTLKDNSKVNMSQ
                     LEKVDGVMGVRQNVQCQVIIGNDVVEVYDEVKALLGDNVSNTEEAPKQKQKLGSVLLD
                     FIISIFQPLIPAIAGGGVLKSLLLLASILGLISDQSQTYQILNLVGGAPLYFLPILVA
                     MTTANKLKVNQLVAVSAVGALILPEMTAMLAEGATFLSFGLDNIAYASQVFPAILTVL
                     FYAQLEKWFTKVSPKPIRIFFVPMMSLLITVPIALLLLGPLGYNVGTAFSSIIVSLYN
                     QFGWVATGVLAAVLPLMVVTGMHKAMIPYAVSSMSELGAELLYLPASLAHNISEAGAC
                     FAVSIKTKDKKLKSTAVSAGISALFGITEPALYGVTILHKRVLYAVMFSSLIGGAFAG
                     IVALQAFALVGPGIASITMFADKADPMNLVWAFAVSGISFVVAFAAALVLYKDKEVVN
                     EVETKMETAVVSDAVLSSPVNGKVISLSEVKDTVFSSGMVGDGVAIIPEEGILVAPED
                     GEITMVFETKHAIGLKTANGAELLFHIGIDTVQLEGKGFEAFVRSGEKVAKGQKLIAF
                     DIDLIKEAGFDPTVICVVTNKDNYTIRSFNLEKLVTNDSDLLAISPIA"
     gene            2412..3878
                     /locus_tag="PW004_002489"
     CDS             2412..3878
                     /locus_tag="PW004_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016620473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL2727420.1"
                     /translation="MTNTTSFPKGFLWGGAIAANQVEGAWNVDGKGLSVADMATYKPN
                     LDNKDFAAHMHVTLQSIKEASEDKSDKWYPKRRGVDFYHHYKEDLALFAEMGFKTLRL
                     SIAWTRLFPTGEELEPNEKGVAFYENVFKEMQRLGIEPIVTLSHYEMPLALAVKYNGW
                     VERRVIDDFVRFANVCFDRFGKYVKYWLTFNEIDSIHRHPFTTAGIIQEKSAEGQEAQ
                     DIYQALHHQFVASALVTKDAHEKIPGSQVGCMLTKLMTYPLTCAPEDVELTLKKNLEN
                     NFYADVQVKGEYPALIEKDLENRGIQIKMEDEDREILKKHTVDFVSFSYYMSMAESAD
                     PNAERTPGNTVLGVKNPHLPSTDWGWQIDPKGLKISLIELYDRYQVPLMIVENGMGAV
                     DQVVDGKIHDPYRIDYFKAHFQQMKEAIDEGVDLIAYTSWGPIDLVSAGTSQMSKRYG
                     FIYVDADDEGNGTYNRLKKDSFNWYKEVIETNGASLFE"
     gene            3915..4757
                     /locus_tag="PW004_002490"
     CDS             3915..4757
                     /locus_tag="PW004_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL2727421.1"
                     /translation="MISIKKVLNSSVVLVEENGKEMIALGKGIGFGQKAGDEISGDKV
                     DKIFLPFEEKKSSQLLELVEEIPIEFFEITRTIVFEAEKGLDKKLNPGVYLTLTDHLH
                     FAVERAQKGVNIANRLYWEIKIYYPKEFQIGKYALTLLNEQYGIDLPKEEASNIAFHL
                     INAQSEDPDSGAGFKYAKMIGGIVNLVRYSLQKDIDTNSIHYARFITHVRFFVERYYS
                     DALLDDGEEEFYNQMWILYPSAMESAAKIKDYIEKVYHKTIPNDEVVYLGVHINRLMK
                     HANG"
BASE COUNT         1607 a          784 c         1029 g         1465 t
ORIGIN      
        1 aagcgctaat ctaattcact gtattatcat tttttagtag tatagcacaa ttatgaatgc
       61 cttttcttta tatcaaataa agtttatata aaaaacctca tcctgcaaga aagaaaaaat
      121 gaacccattc tttttatact aggtcttaaa aagacagaac atctgctaga agatagaaga
      181 gaataagagt tatatatgtt ggaagatatt cttagaaaaa agacaaaact tttagaacac
      241 gaaaactata ttgaacgaat aaaatgaaaa aatgattaaa aaatcatttt attatttacg
      301 tttacattct aatatgttaa attgaatata acttaaacag gcatgtgact ggtaatgcag
      361 gcaggaccta aataagttga ataacaatgt atgcgtttca atacacgttt gccttgttgt
      421 caatttattt agtttctgcc tgttttttgt gttgaggatg gccacttcga ctcaaaagtt
      481 catcactcaa aaaagaaaag agggaaacac catgaattac aaacaaacag cagaagaagt
      541 attgcagtac atcggtgggg aagaaaacat atcccatttg gagcattgct caactcgttt
      601 acgttttacg ttaaaagaca acagcaaggt aaatatgagc cagttagaaa aagtggatgg
      661 cgttatgggc gttcgccaaa atgtgcaatg ccaagtaatc attggaaacg atgttgttga
      721 agtgtatgat gaagtgaaag cgttattagg tgacaatgtt tcaaatactg aagaagcacc
      781 aaaacaaaaa caaaaattag gttcagttct attagatttt attatttcta ttttccaacc
      841 tttaattcct gcgattgcag gtggaggagt attgaagtcc ttgttgctac tagcttctat
      901 cttagggctg attagtgacc aatctcaaac ctatcagatt ttaaatttgg tcggcggggc
      961 accgctttat ttcttgccta ttctggtggc aatgactacg gctaataaat tgaaagtcaa
     1021 tcaattggtt gcagtatctg ctgtcggtgc attgatcctt cctgaaatga cagctatgtt
     1081 ggcggaaggt gctacattct tatcttttgg attggataat atagcgtatg catctcaagt
     1141 attcccagcc atcttaactg ttttatttta tgctcaactt gaaaaatggt ttacaaaagt
     1201 ttcaccaaaa ccaattcgta ttttctttgt acctatgatg agcttattga ttacagttcc
     1261 tattgctttg ttgcttctag gaccattagg ttataatgta ggaacagctt tctcttctat
     1321 cattgtgtct ttgtacaatc aatttggatg ggttgcaacc ggagtcttag cagctgtact
     1381 gccattaatg gttgtaacgg gtatgcataa agcaatgatc ccttacgctg tttcatccat
     1441 gagtgaattg ggtgcagaac ttctttatct cccggcttca ttggcacaca atatttcaga
     1501 agctggcgca tgctttgctg tcagtatcaa aacaaaagac aaaaaattga aatctacggc
     1561 tgtttctgcg ggtatatctg cattattcgg aattactgaa ccagctcttt acggggtaac
     1621 gattctgcac aaacgtgttt tatatgccgt tatgttttct agcctgatcg ggggagcatt
     1681 tgcaggaatc gttgcacttc aagcctttgc tttagtgggt cctgggattg caagtatcac
     1741 gatgttcgct gacaaagctg acccaatgaa tctggtttgg gcatttgcag tatcggggat
     1801 ttcatttgtg gttgcttttg ctgcagcgtt ggttctttat aaagataaag aagttgtcaa
     1861 tgaagttgaa actaagatgg aaacggccgt tgtttcagat gctgttcttt caagtcccgt
     1921 gaatggaaaa gtaatctcac tatcagaagt aaaagacact gttttttcta gtggtatggt
     1981 aggtgatgga gtagcaatca ttcctgaaga agggatatta gtagctcctg aagatggaga
     2041 aatcacaatg gtatttgaaa cgaagcatgc tattggttta aaaacggcta atggtgcaga
     2101 acttcttttt catattggta ttgatactgt tcagttagaa ggaaaagggt ttgaggcatt
     2161 tgttcgttct ggtgaaaaag tagccaaagg acaaaagctg attgcatttg atattgattt
     2221 aatcaaagaa gcaggattcg accccacagt tatctgtgtg gtaaccaata aggataatta
     2281 cactatccgt tcatttaacc tagaaaaatt ggtaaccaat gatagtgatc tcttagctat
     2341 cagtccgata gcttaaacac aagatagtga aaaaaaatat attgaatgaa ggaatagagg
     2401 agaatagaat aatgacaaac acaacaagtt ttccaaaagg atttttatgg ggcggcgcga
     2461 tagcagcaaa tcaagtagaa ggtgcttgga atgtagacgg taaaggtctt tcagtagcgg
     2521 atatggctac ttataaacca aatttagaca acaaagattt tgctgctcat atgcatgtca
     2581 ccctgcagtc tataaaagaa gctagtgaag acaaatctga taaatggtac ccaaaaagaa
     2641 ggggcgttga tttttatcat cactataaag aagatttagc tttatttgcc gaaatgggct
     2701 ttaaaacctt gcgcctctct attgcttgga ctcgcttgtt ccctacagga gaagagctgg
     2761 aaccgaatga aaaaggggtt gcattttatg aaaacgtctt taaagaaatg caacgtttag
     2821 gaattgagcc gattgtgaca ctatctcatt atgaaatgcc attagcttta gcagttaaat
     2881 ataatggctg ggtggagcgt cgagtaattg atgattttgt tcgcttcgct aacgtttgtt
     2941 ttgatcgctt cggcaaatac gtaaaatatt ggctgacatt taatgagata gacagcatcc
     3001 accgtcatcc atttacaaca gctggaatta tccaagagaa aagcgcagaa ggtcaagaag
     3061 ctcaagatat ctatcaagca ttgcatcatc aatttgttgc gagtgcgtta gtgacaaaag
     3121 atgctcatga aaaaattcca ggaagccaag ttggttgtat gttaacgaaa ttgatgactt
     3181 atccattaac ttgcgcacca gaagacgttg agctgacctt gaaaaagaac ttagaaaata
     3241 atttttatgc agatgttcaa gttaagggag aatatcctgc tctaatcgaa aaggatttgg
     3301 aaaatcgggg aattcagatt aaaatggaag atgaagatcg agaaatttta aagaaacata
     3361 ccgtagattt tgtttctttc agctattaca tgtccatggc agaatcagct gatccaaacg
     3421 cagaacgcac accgggaaat actgttttag gggttaagaa tcctcatttg ccttcaacag
     3481 attggggctg gcaaatcgat ccaaaaggtt tgaaaatttc gttgatcgaa ctttatgatc
     3541 gttaccaagt accgttgatg attgtggaaa atggtatggg agcagtcgat caggtagtag
     3601 atggtaaaat ccatgatccg taccgcatcg actactttaa agcacacttc caacaaatga
     3661 aagaagcaat cgatgaaggt gtagatttga tagcttatac tagctgggga ccaattgatt
     3721 tagttagtgc aggaacttct caaatgtcta aacgatatgg atttatttat gtggatgcag
     3781 acgatgaagg aaatgggaca tataaccgct tgaaaaagga ttcgttcaac tggtacaaag
     3841 aagtgatcga aacaaacggt gcatccttat tcgagtagtc tcgatctttt taagcagcga
     3901 tagggggata atgagtgatt tcaataaaaa aagtattgaa ttcaagtgtg gtacttgttg
     3961 aagaaaatgg caaagaaatg attgcattag gtaaagggat aggctttgga cagaaagctg
     4021 gggatgaaat atcaggagat aaagtagata aaatatttct gccttttgaa gaaaagaaat
     4081 cttcacaact attagagtta gttgaagaaa tacctattga attttttgaa atcactcgta
     4141 ctatcgtttt tgaagcagaa aaaggattag ataaaaaact gaatcctgga gtctatttaa
     4201 ccttaacaga ccacctgcat tttgcagttg aacgcgcaca aaaaggggta aatattgcaa
     4261 accgtttgta ctgggaaata aaaatctact atcccaaaga atttcaaatt gggaagtacg
     4321 cactcaccct tttgaacgag caatatggaa ttgatcttcc taaagaggaa gcctcaaaca
     4381 ttgctttcca tttgatcaat gcacaatcgg aagatcctga tagcggcgct ggattcaagt
     4441 atgcaaaaat gatcggcggg atcgtcaatt tggtccggta ttcgttgcag aaagatatcg
     4501 atactaattc gatccattat gcccgcttca ttacacatgt acgtttcttt gtagaaagat
     4561 attattctga tgctttgttg gatgatggtg aggaagaatt ctacaatcaa atgtggatct
     4621 tatacccgtc tgccatggaa agcgcagcta agatcaaaga ctacattgaa aaagtttatc
     4681 ataaaacaat tccgaacgat gaagttgtat acctaggtgt ccacatcaat cggttgatga
     4741 aacatgctaa tgggtgagcg ctcagtaata ctattgatgc ttttaaaatc tttagagatg
     4801 gattatagta aaaacaaggt gatttccagc tgtttgttat ctataagaga catttaaaaa
     4861 ggattaacgt aagataataa aaaat
//
LOCUS       DANKKG010000152         4805 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000152 DANKKG010000000
VERSION     DANKKG010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.152"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(31..1641)
                     /locus_tag="PW004_002491"
     CDS             complement(31..1641)
                     /locus_tag="PW004_002491"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HDL2727422.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(2834..4174)
                     /gene="glnA"
                     /locus_tag="PW004_002492"
     CDS             complement(2834..4174)
                     /gene="glnA"
                     /locus_tag="PW004_002492"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HDL2727423.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(4202..4594)
                     /locus_tag="PW004_002493"
     CDS             complement(4202..4594)
                     /locus_tag="PW004_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL2727424.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT         1408 a         1036 c          789 g         1572 t
ORIGIN      
        1 ctacttttct ctttctatca agttcaaaaa ttattttgtt tcgtgatctg ctaatgatgc
       61 tccgacaaat gctttgatta atttttgcgg gcgatttgga cgagaaatca attctgggtg
      121 gaattggcat cccacaaaga attttttctc tggcagctct acgatttcta ctaaacggtt
      181 atctggtgag acacctgaaa agactagccc ttgttcttca aacaattgac gatatttgtt
      241 attgaactca tagcggtgac ggtgacgttc ttgcacaaca tcggctccat catatgcttc
      301 agccgtcttc gttccttttt tgattttaca tgggtataat ccaaggcgta aagttccacc
      361 caaattctcg atgttttcct gatcagccat taaatcaata atattgtttg ctgtatctgg
      421 attcgtttca gctgatgacg catcttctaa tccaacgacg ttacgtgcaa attcgacaca
      481 agccatctgc ataccaagac aaatacctaa aaacggaacg tcattttcgc gagcataacg
      541 aatcgcttcg attttccctt ctactccgcg atcaccaaat ccaccaggaa ccaagattcc
      601 atctgcgtct ttcaaacgtt cttctacatt ttctctcgtt aattcttgtg ctttgatcca
      661 atcgatctcg atgtctgaat tataggcgaa accagaatgt ttcaatgctt caactactga
      721 gatatatgca tctggtaact cgacatattt accgacaagc gcaatccgta cttttttctt
      781 caagttcaag acacgttctt ctaattcttt ccattctgtc atgtctgctt ctggtgtatc
      841 tagttttaaa tggtcgcaaa caatttgatc cattccttgt gcttgtaaaa ctaatgggat
      901 ggagtaaagt gtatctacat ctggcgactc gataactgct tctggttcga catcacaaaa
      961 ttgtgccaat ttgtttttca cgctttgaga aacaggttgc tctgtacgta ccaccaaaat
     1021 attcggttgg atacctaaac cacgtagctc ttttacactg tgctgcgttg gtttagtttt
     1081 catttctcca gcagctttca agtaaggaat caatgttgta tggatataca taacattatc
     1141 tgtaccgaca tctgctttca tttgacgaag tgcttcaagg aatggcaaag attctatatc
     1201 ccctactgtt ccccctactt ctgtaatgat cacatctgaa tcagtcattt tagctgctcg
     1261 catgattttt tctttgatct catttgtaat atgaggaata acttgaacag ttgctcccaa
     1321 gtattcgccc ttacgttctt tacgcaaaac ttcagaatag atttttcctg tcgtcacgtt
     1381 ggagtatttg ttcaaattga tgtcaataaa acgttcgtag tggcccagat ccaaatctgt
     1441 ttcagcacca tcatctgtta cgaatacttc tccgtgctga tatgggctca tcgtccccgg
     1501 atccacgttg atgtatggat caaatttttg gatagttact tttaagccgc ggtttttcaa
     1561 taagcgtcct aaagaagctg caacaatccc ttttccgatt gatgaaacga caccgccggt
     1621 cacaaaaata tattttgtca taagttcaaa aactcctttt taatttatgt gcaggaaaag
     1681 tctcagacaa taaacaaagc tccctattct tcaataagaa tagggagccg aaatttctgt
     1741 aaacttctga ccctctaaaa gggtgcccaa ggtgtataat acaagctgac taagggaaag
     1801 tcaagtactc aaatgaatac tatccgctta caattttttc tttttcgata tcctttgaga
     1861 aattatttct taagtccttt atgagtgcaa aaaaaattta acccacataa taagcttgat
     1921 ataaacatag ctaacgttaa acctaagcaa atcagaaaat cgttactgaa aaattttaag
     1981 tgaaataata gataaaaaaa taaaacaaac attatgatag ataatatccc acctaacaaa
     2041 gaactacttt ttcctacttg cagcaacagt ttatatagct tatcgtagaa acaatctttt
     2101 tctttcaccc ttatttacct cctttacgta aaaaacgtag tccacagttc tcggattttt
     2161 ggcatatctt ttctattaaa taatatcaaa aacaaaacaa ttccactaat tttttcaaag
     2221 atgacctttt tttcaacaaa agtgccttgt agggattcat tgaaggtaac gaatcacata
     2281 agtaaaaaat aaggagatgc gacaaaagtc gtgcctcctt atttctaaat aaacggtgtt
     2341 ctcctgccaa ctcctcggtc acaagtcaca atatccatca aacatgagaa gctgtttttg
     2401 aaattatttc ttgtgattcg gagttaaatg gtaggctcac aacctctgga taaacggtat
     2461 tcaaaagcca gtttctcggt attaagccaa atttatgcaa aaatgtagaa agccattttc
     2521 acaaaatttt tcttaatact cgaaactaaa cgtctttttc aaaacctctt ggtaaacggt
     2581 gttcaaaagc ttgcatctgc ggtaataagt tcagccaatt caaaaacaag aaaagctgtt
     2641 tttgcttggc tattcttatt acttgttgca gggcagcttt ttcacaacct ctccactaaa
     2701 cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata tgaggaacta
     2761 tttttccgtt agtttctcta atactcaggt caaaacgctg atttcacagc ctctaattca
     2821 cggtattcct gttttaataa agttctagat attgttctct ttcccactcg gaaacagttt
     2881 gtctgaatgc cgcccattcg agacgttttg cttcaacaaa atttgagaag atatgttgtc
     2941 ctaatgcgtc aatcattaca ttatccttac gcaattcttt gatagcatta tggatagttg
     3001 aaggtaagtc ctggatatgt gcagcttctc tttcttcctc atccattaca taaatgttac
     3061 ggtcaacagc tgctggcggt gtgatctgat tacggatacc gtctagtcct gcttgcaaca
     3121 atactgccat tgccaagtat gggtttgcag atggatcaac tgaccgcaat tccaaacgtg
     3181 tggataaacc gcgagattct ggtacacgta ctaatggcga acgattacgg ccactccatg
     3241 cgacataaac aggcgcttca taacctggaa cgagacgttt ataagaattg actgttggat
     3301 tgcaaacggc tgtgtaggca cgagcatgtt tcagcaaacc acctaagaaa tgataagctg
     3361 tttcacttag ttccaattca ccgtctttat catagaaaac atttccttca tcattgaaca
     3421 aagacatatt gcaatgcata ccagaaccat tgatcccaaa taacggtttc ggcatgaaag
     3481 tcgcgtgaag tccatgcttg cgtgcaatcg ttttgacaac gagcttgaag gtttggatat
     3541 tatcacaagc atctaccaca tctgcatatt taaagtcaat ttcatgctgt cctggcgcta
     3601 cttcatgatg tgaagcttcc acttcaaagc ctagactttc taattccaac acgatgtcac
     3661 ggcggcaatt ttcacctaaa tctgttggtg caaaatcaaa ataaccacct ttatcattca
     3721 aggtcgttgt gatcccaccg tcttcgtcta atttaaataa gaagaattct ggttcaggtc
     3781 ctaaattgaa agaggtaaaa ccaaggtcgc gcatatcttt caatgctcgt ttcaaattcc
     3841 cgcgaggatc gccggcaaat ggtgtcccat ccggattata gatatcacaa attaaacgag
     3901 caacttttcc atgagcactt tcccacggga agatcatcca agtggagaga tctggataca
     3961 aatacatatc gctttcttca attcgaacaa atccttcaat cgaagaaccg tcaaacatca
     4021 ttttattttc taatactttg tcgatctggc tcactggaac ttctacattt ttgatcgtac
     4081 ctaagatgtc tgtgaacatc aatcgtaaaa atcgtacgtt ttcttcctca atgattcgtt
     4141 tgatttcttc acctgttatc tgtttcttta ccatggtatc cctcttcctt ttttaattta
     4201 ttcagtcaaa gaattgtctt tgcaatcctt gcgactgata atgacctgtt ttgcttaacc
     4261 caccttgcgc caaaatctca tcacgaaaaa tttgtcgaac gtcatgatct gtcaacggct
     4321 tcttgttttt tgcttcttgt tttttcgcct gtgccatttc gtatacacgt ttgatcccag
     4381 ccatattcaa tccctctgaa agatagtctt taatctcaag caacatatca atatcattca
     4441 gcgaatacat acgtcggttt ccttcgcttc gttccggatg aactaaatct tgttcttcat
     4501 aatagcgaat ttgccgagca gacaaatcag tcagcttcat aactgtacca attggaaata
     4561 cggacataga tcggcgcaat tccttttctc ccatcagacg tcccctttcg ttttataagt
     4621 tggctaaagt gtaccaatta aaaaaaatat tgtcaactgc atatgttagg ttttctaaca
     4681 tgagatctct attttttata aaataacaaa aaaataagaa cacaaaaaag gtatctagtt
     4741 gattttcgta ccagatacct tttatttttt attatactgt actatccttt attagtgttt
     4801 ttatt
//
LOCUS       DANKKG010000153         4805 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000153 DANKKG010000000
VERSION     DANKKG010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.153"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            59..174
                     /gene="rrf"
                     /locus_tag="PW004_002494"
     rRNA            59..174
                     /gene="rrf"
                     /locus_tag="PW004_002494"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(227..697)
                     /locus_tag="PW004_002495"
     CDS             complement(227..697)
                     /locus_tag="PW004_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HDL2727425.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(856..1314)
                     /locus_tag="PW004_002496"
     CDS             complement(856..1314)
                     /locus_tag="PW004_002496"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HDL2727426.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1485..2630)
                     /locus_tag="PW004_002497"
     CDS             complement(1485..2630)
                     /locus_tag="PW004_002497"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HDL2727427.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2643..3560)
                     /locus_tag="PW004_002498"
     CDS             complement(2643..3560)
                     /locus_tag="PW004_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HDL2727428.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3988..4551
                     /gene="efp"
                     /locus_tag="PW004_002499"
     CDS             3988..4551
                     /gene="efp"
                     /locus_tag="PW004_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDL2727429.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            4652..>4805
                     /locus_tag="PW004_002500"
     CDS             4652..>4805
                     /locus_tag="PW004_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727430.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAK"
BASE COUNT         1506 a          882 c          920 g         1497 t
ORIGIN      
        1 tgacttcaaa tccagttttg agtgaacaaa atttactcaa taaaagataa caccacagtg
       61 tggtggcgat agcgagaagg atacacctgt aaccatgccg aacacagaag ttaagcttct
      121 tagcgccgat tgtagtgagg ggttgcccct tgtgagagta ggacgtcgcc acgcaaaggc
      181 aaaaagatga actagaaata gttcatcttt ttttattttt attaagttaa agaacggatt
      241 caattgcagc ggtcattttc aatggagaag tttgtggtgc aaatcgttcg attactttgc
      301 catttctatc aatcaaaaat ttagtaaaat tccattttat tgctttagtg acagtaccag
      361 gagcttcatt tttaagataa gtaaaaagag gatctgtatt ttctccattt acgtcaatca
      421 attgatgcat agggaaggta acgccataag tggttttaca agcttctgct gcttcagtcg
      481 ctgaatctag ctcttgttta aattgattgg aaggaaagcc taagataacc agaccttgtg
      541 tctgataagc ttggtaaagt tcttctaatt gtttaaattg aggtgctaga ccgcattttg
      601 ttgcagtgtt aacaatgatt atcggtttgc ctgcatattt tttcaatgaa tagacagtgc
      661 cgtcttctaa agtagtttca aaattataaa tagacataaa gttttctcct ttctatttta
      721 tataataaaa agagcgctaa actgaaaact agctatttgt tttcccgttt agaactcttt
      781 tcagacaata aatatttaat aaaatacgaa gataagatag ctatattgct tctcatcttt
      841 acgttctttt agaaactatg cttctacttc agaccattca tcgcgttttg ctaaaaattc
      901 acgagccaaa gcttgtgcac cttctagtgt atggctggca gcccagccac attgtgtttc
      961 attgcttgca ggtacttcag tagcttcaag aacatctttc attgttgctt caagaacatc
     1021 taaaatgtct tcgtagcttt catgattcaa tacagtcaag taaaagcctg tttggcatcc
     1081 cattggagac cagtctacga tgtaatctgc gtgattgcgg attagttcgg cagtcaagtg
     1141 ctcaagtgaa tgtagacttg gcatgtccat gtgttcttta tttggttgtt taaagcggac
     1201 gtcgtattta atgatgacat cgccatgcgc acccgttttt cgatcagcta cacgtacata
     1261 aggtgctttg acttttgtat ggtcaagatt aaaactttcg acattcattc tcatttgtaa
     1321 gactcctatc tgtttgttca ttcgttttga tgcttttcac taaagaatta tttagtgaaa
     1381 tttacaaaaa cgtctttcta cttgattcta acacttatcg tttccaaggt gtagcaatct
     1441 gaaatatttt ttagattttt ctgctaagtc gtttattggg acttctagtt cttgattctg
     1501 tcaaatgcct gagctaaatc gtttatgaga tcctgaccat actcaattcc tacagaaaga
     1561 cggatcaagc cgtcctttat gccagcctct tctcgttttt cttttggaat agacgcatga
     1621 gtcatgacag aaggaatttc cactaaactt tctacgccac ctaaactttc tgctaaagta
     1681 aatagttgga gtgattcaac aaatggaata gcagattctt cattttttaa agtaaaagaa
     1741 atcattccgc taaagccgct catctgctgc ttagcaactc catggagttc atgatcgggt
     1801 aatcctggat agtagacctt ttctactgct ggatgagaaa atagaaaatc agctaccaca
     1861 aaagcatttt tttgatgttc ctccatacga acacttagtg tcttgattcc gcgctgaagt
     1921 aaccagctat cttggggacc aagtacggct ccgatagcat tttggtaaaa tccgatctga
     1981 tcggctagat ctttatggtt agaagtcact aaaccagcta ctacatcgct gtgtccgcct
     2041 agatatttgg taccgctatg gacaacgata tcagaaccga gatctaaagg tctttgaaaa
     2101 taaggagtag caaatgtatt gtcagcaata actatcaatc catgttgatg agccagagtg
     2161 gcactttttt ctaaatcagt aatctttaga agcggattgc taggtgtttc aagataaagt
     2221 gcttttgtat tgggtttgat ggattgttct attttatcaa gattactcgt atctatgatc
     2281 gtgtactcga gaccattttt agtaagaact ttgtcaaata gacggaatgt tcctccatag
     2341 acatcatctc ctaataagat atgatcgcca gcttgaaaaa gagaaaaaac ggcatgaatc
     2401 ccagaaagac cagaagaaaa agcgaatcct cgaacaccgc cttccaaatc agcaatcagt
     2461 tcttccaatg caaaacgagt aggattacca gatcttgaat attcatattg tttaggctgg
     2521 ccgacgccat cttgccgata agtggatgtt tgataaatag gtacactgac agctcctgtt
     2581 gtgggatctt cactgatgcc tccgtggatc aattttgttt gaatgtgcat gtttctttcc
     2641 tcctataagt aaatgttttt tgataaatag cggtctgctg cgtctggaaa aatcgtaacg
     2701 acacgatgac caggtggtaa acgacgaatt tctcttaatg ctgctgcaaa agccgcacca
     2761 ctcgaacttc caactaatag tccttgttct cgtgctaact gcctagtata gttaaaccct
     2821 tctacatcag agattgtctc gatctgattg attgaaaggg gagaaagaaa cggcggaatg
     2881 aattctacac cgataccctc aatttcgtga ggggctggat caccaccatt taacacagag
     2941 ccctctggtt ctacaccgat tattcgaatg tcgggatatt tttctttcaa aaaggtagaa
     3001 gttccagcaa aggttccacc actcccaatt cctgctacaa aactatggat attttctttg
     3061 atttcatgaa agatttctgg ccctaacgta tgatagtaag cagcaggatt atctttattt
     3121 tcaaattgaa gaggcagata gctgtttgat atctcctctg caagtttttt acttttgctg
     3181 atggcaccga tgattccttg ctcactagga gaatggatga tctttgctcc caatgctttc
     3241 atcaattgtt gtttttctaa gctaaacttc tctggaacaa caaaaaccgt tcttaatttg
     3301 tatgtgagtg cggccagagc gatcccaatt cctgtatttc cggcagttgg ttcgatgatc
     3361 gtcgtttttt cattgatttt tccttgggca aatccttctt tgatcaaata actgcccaat
     3421 ctatctttga tacttcctcc aggatttaaa tattctagtt ttgcataaat agcagaacct
     3481 ttaggcatag aatagtgctg attgtcaaag cgataaagcg gtgtggaacc aatagcttct
     3541 gtaattgtcg agataatcat gtgggcctcc taaaaaaaga taataaaaaa aggagctgac
     3601 tattaaatca gctccttgaa agacatagag aaaaattgag tagataaaaa gataagttac
     3661 ctggtactca ttctatgaag ggcaattaat agacatgaca aatagtatgc tgcttttatg
     3721 caccatacaa cagcaacgat tcaattgttt ttggttgctg actgtcatgt gtatccctcc
     3781 aagtaaaatt tttattaacg atagcatgaa aaaaaataaa agtcaaaggc ttgaaagaaa
     3841 aaaatatttc gatagcatcg tttacccgtg tttatttttc tgacaaatag gcatgagacc
     3901 ttaaaagata gagaatactc tagttttctg gccggttgtc tgatacaata aagttgatta
     3961 tgataaagaa aagaaggagc aaaaataatg atctcagcaa gtgatttaag agccggtatg
     4021 actttcgtac aagacggcaa actgattaaa gttctggatg caagccacca taaacctggt
     4081 aaaggaaata cagttatgcg tatgaaactg aaagatgtcc gttcaggtgc aacatacgac
     4141 acaacgttca gaccagaaga aaaatttgaa aaagcacata tcgacacaaa acctgtacaa
     4201 tatttgtaca cgatggatga tacagctttc ttcatggatt tggaaacata tgagcaatat
     4261 gaaattcctg tagaaacagt agaagaagaa atgaaatata tcctagaaaa catggaagta
     4321 aaaatccaat ttttcggtag cgaagtcatc ggcgttcaat taccaacaac tgtcgtatta
     4381 cgtgttgtgg aaacacaacc ttccatcaaa ggtgcaacag tcactggttc aggtaagcca
     4441 gcgacaatgg aaactggctt agtagtcaat gttcctgact tcatcgaaac agatgaattg
     4501 ttggaaatca atacagcaga aggttcttat gtaaaacgtg cgtcaaaata attcgctaac
     4561 ggattgataa gaatttgaaa acagagcttg acctcttgaa aggcagatca tcggatctgc
     4621 ctttcaagag gttttttgtg gaatcagcaa attgaaaaat aaaaaagtat ttgattttat
     4681 ttatattttg aaagatgaat ggcaaaagct agagaaagaa aaaactgcac ttatttatga
     4741 agaaattgaa atgattcata aaattgaaaa tatccgaact attccgaact atattgaagc
     4801 gaaaa
//
LOCUS       DANKKG010000154         4801 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000154 DANKKG010000000
VERSION     DANKKG010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.154"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(82..1890)
                     /gene="pepF"
                     /locus_tag="PW004_002501"
     CDS             complement(82..1890)
                     /gene="pepF"
                     /locus_tag="PW004_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HDL2727431.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2066..3064)
                     /locus_tag="PW004_002502"
     CDS             complement(2066..3064)
                     /locus_tag="PW004_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HDL2727432.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3290..3943)
                     /locus_tag="PW004_002503"
     CDS             complement(3290..3943)
                     /locus_tag="PW004_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HDL2727433.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4275..4673)
                     /gene="spxA"
                     /locus_tag="PW004_002504"
     CDS             complement(4275..4673)
                     /gene="spxA"
                     /locus_tag="PW004_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HDL2727434.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1410 a         1007 c          744 g         1640 t
ORIGIN      
        1 taaaaaaata cgtcgactcc cttacaagag agccgacgta tttttttcgt ttctattcga
       61 gaaaatcaaa aatagaaagc cttataaacg atcgatcaat tcttctaatt catttagacg
      121 ttgttcaaac atcgacattg catcttcaat atatgcagcc tgtgtcatat caactcctgc
      181 ttttttcatt acttccacag gataatcgct gttacctgct tttaggtaag ccaagtagtt
      241 ctctagtgcc tctggttcct ggtttagtat tttcttcgcc agtgctgaag cagcagaaaa
      301 gccagtcgag tattggaaaa catagtaatt ataataaaaa tgcggtatcc gtgaccattc
      361 gaatttaatt tctgggtctt cttcgactgc tggaccataa tattttgcat tcaatttacc
      421 ataactatca cttaggtatt cactagtcaa tggcacacct ttttcatctt ccgtatgcat
      481 gaaatgctcg aattcagcga attgtgtctg gcggaaaact gttcctttaa acccatccaa
      541 gtaatggttg agtacataag cccgtacacg agggtctttt tctgtttcca ataaatactc
      601 cgttaggata ttttcattcg ttgtcgaagc aatttctgct aaaaagatgg agtagtcgcc
      661 gtacacataa ggctggttcg aacgagtgaa ataactatga acactatgtc ccatttcgtg
      721 gacaagcgta aatagctgat ccagcgtatc atgccaattc aataaaatat atggatttgt
      781 gtcatagctt cccgaagaat aagcaccgct tcgtttccct ttattttcga caacatcgat
      841 ccaacgttca gagaatgctt tttctacgat ggccatgtat tcttcaccca ttggtttcag
      901 tgcttctaaa gctttttctt ttgcttcttc gtacgtaaag gtaattggag cttcccctaa
      961 gaccggtgta taaagatcat acatgtgcag tttttccact tctaataaac gtttccgtaa
     1021 ttccatgtat cgatgtaaca aaggcaaatg cttgtttacc acgtctacca aagtatcgta
     1081 tacactttca ggaatatgat tattgctcaa agacgcttct ctggcagagc tgtaattacg
     1141 gactttcgct ttaaaattat gtccttttat atgtgtgcct aaagtagaag caaatgtatt
     1201 tctaaattgt tcgtaaacac tgtacaaccc cttaaatgct gcttcgcgca cccttcgatc
     1261 cgtgctttct agcaactgac catacacgcc atgagataat tggactattt caccattttc
     1321 tccttcaatc gttggaaaaa ctagatctgc attattcaaa acagcaaatg tatcacttga
     1381 agcatcaaag atttcacctg ctccagcaag aagagattcc tgctcagcag ataagacatg
     1441 ggctcgatta tctactattt gctggatata atggcgatag acttccaatt ttggttcttc
     1501 tttgaaatat tgccaaatct ggtcatctga caattgcaat atttctggtt caaaccagga
     1561 aacggcttca ctaactttcg aaaacagact gcttgctctt gcataaagcg cttggtaatc
     1621 tgtatttcca gtgtcttgat cgtttttaag gtgcgcatat acataaatga cttcagtttg
     1681 gcgataaacc ctcaatacga attcaatcgc atttaaaaat tgagaagcgc cttgatcaag
     1741 tgttcctttg tgtttttcag attgttttaa ctcttctgat aattccaaat atttctcatc
     1801 aaactcttgg tcgcttgaaa agatcttggt caagtcccaa gttaaatttt caggcaattc
     1861 ttctcgtttt ggcaactgct ttacttccat gaaatccact ccttttcttc ccttagctct
     1921 atctatcaaa atagatcata gtctaacatc cacatgtcac agcatctaac ttttctactc
     1981 attcatgtgt ctgcttccat caattttagc ataactaaat cttgttttac atagaaaacc
     2041 gattcctagc ctatttttat acttactatt ttttcaaaga agacaagtgt atgcattcgt
     2101 taaaaaactg ctgatagacc atctcttcat caatcatagg aaaaagccat tcccttttat
     2161 gttcttttat ttggcaaagc catgagttga aatcaggaac tttctggttt tccaaggctt
     2221 cttcgtacaa cattcgataa agaaaaacct gttcttgtaa ataaaagaaa aaccgacttt
     2281 tttgatagat ccacgatgat agatgcaaaa gatgtttatg acgcaagtag cattgttctt
     2341 gtatggatac tgtattcttt tgcttgtgaa taagttggcg tgtcagccat ttcttcccat
     2401 cttcactcac tttttctatt tttttcattt ggttcatttt aatttcgtta ctagtgaata
     2461 tttctttaag gtccctacta gaaaaagtcc agcagctact gcaaaaggaa atatgccctg
     2521 atacagtctc tgtaatatga tgatacaaat aaagcttttg tttcttgaga tcagctttcc
     2581 agcaatgcac tcctcttttt ctattatagt agcagcaact tttttctatc aatgagaatt
     2641 gcgattgtcc aagaaaagaa ttaccaagta tccaccatac ttggtaactg ttattcaaat
     2701 aattttgggt tctttcaata aatctctggt gagacagtct agaacattgg atttctattg
     2761 ctagattcgc caacaaaata tccggccttt gccgcaacct cggcaaatag gcttccaaaa
     2821 atactggttc tactgactgt cccagccaat ccatgaagca ttcctttaac tgtaagtgct
     2881 cttttgattc tctttcacta aagaccgaac agttatctga tttttgatgg gcaaaatgag
     2941 cacatttttt ctttcccttt ttcaaaatca ctctttttcg acagccacga caataatatt
     3001 tctttccttt ttctgcatgt ttagcaaaac agcacttatt atattcattc attgcaagca
     3061 gcatttttct ccctctgaac ttatttacgt ctaagaattt atttacacga aaaaagaggg
     3121 cgtgaaaaag ctattctgca tcaagtaata agaacagcta aacaaaaata gttcccaata
     3181 tttttcgttt ggttgggctt gttaccgcag atgcaagctt ttgaacacat ttatacgaaa
     3241 aaaagactgc gacacaattt ttgtcacagt ctcttactat aatgctgatt tatacaaaat
     3301 aaaatttcgt tcgctcgata gcattacgcg ccatcagaca ttgtccatat tcattcaatg
     3361 tttctggtgt acgatcgctc ttttcagcaa actccaaaat tcgagcaatc tgattctcta
     3421 catccgcttg gttgagattt tccatccaga agtgaacgac taggtaatag cgctcttcga
     3481 ataaatacaa atcagtccaa gcagactcta gttcgacatc tgttgcaagt tggatcactt
     3541 gttcaaaatc ttcaaaagca aaagttgctt gtctttcctg ttcttctagg gcgcttccag
     3601 ataattcttc ctccggatcg ctgccaataa tctgctgacg aagaaagtca ctcacatctt
     3661 ctgaatcaac agtagaatct tctgattgag aaatttcttc atccggaaga tttttactga
     3721 taaacagttc taatccgtct ccttttggca aaacttggaa agtgacagct tcgcttcctc
     3781 ggaactcctc ttcgatatca acttcttcca aaatactata aaagaaattt tcgatttctt
     3841 tgtgattgcc aagtaaatcc aaaaatgaaa cgccccgttc ctctaaatct tcatgaccaa
     3901 ttagtacacg gatcgtattt tcattaatat gttccatttc cattttgcta cacctcactt
     3961 tgccacaatg tttaatatag attcattgta gcgaaatcag cttaaatgta aagaaaaaaa
     4021 atctcgctgt taagcgaatt taataaaacc agcggatttt tcaacctttc aagctatctt
     4081 atttaactat aacatttttg gcagacgaat actatatagc cgacttttca gctaattttc
     4141 cctctctatt tttcgtgcaa tcatcatctt ctactacctt tttatacatc taatcaagaa
     4201 tactttttgt aaaaacaaaa aaacttggaa aaactatctt tgtaatagtc ttttccaagt
     4261 ttcgtattat ccttctataa tccagccatc aattgtgctt gacgtaattc taattgtcgt
     4321 acatcgcgcg gtaggaaccg acgaatttca tcttcattaa aaccaacttg cagacgttta
     4381 tcatcaatca tgattggacg gcgaagcaat ccaggattgt tttgcacaag ttccaataat
     4441 tcttgtaacg gcagatcatc taaatccatg ttcagttttt ggaatacttt ggaacgtgtc
     4501 gagatgattt cttctgttcc gtcttctgtc atttgtaaaa tcgcttttaa ttcagaactg
     4561 ttcaatggtt ctgaaaaaat attacgttca acaaatggaa tctgatgttc ttgcaaccac
     4621 gcacgagcct ttctgcagga agtacaactt ggggaagtat ataatgttaa caacgtgcac
     4681 cactcctttt cgtgataaga atcacttttt tgtaacagaa gatcataata atctcccatc
     4741 tgtttctatt atacataatc aacttttaaa tagctacctt tttttcataa tttttttaaa
     4801 a
//
LOCUS       DANKKG010000155         4781 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000155 DANKKG010000000
VERSION     DANKKG010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.155"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..524
                     /locus_tag="PW004_002505"
     CDS             <1..524
                     /locus_tag="PW004_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HDL2727435.1"
                     /translation="KTRDKNNFADSSKNELELYKSYKDSKDIKESHNDDLLESLSTKN
                     NKLDEELIETFIEENLLKQIWGESIVNCLKLASNSDYRQFDNWYKKFKYAIRSAEKEQ
                     KIQLKIIYEICNNKYFVDHVREQLSMTLRDLIRRAKTDHRIKQKDDYIFASLKNKALE
                     LIELQISEGIDKQ"
     gene            600..818
                     /locus_tag="PW004_002506"
     CDS             600..818
                     /locus_tag="PW004_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727436.1"
                     /translation="MIKNRLIDQVWCLDQNTDSLELIETICFNTIVLDLRAENDNCVT
                     HVIINQKMAQKLSESLRKWAKGGNDENN"
     gene            805..3483
                     /locus_tag="PW004_002507"
     CDS             805..3483
                     /locus_tag="PW004_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727437.1"
                     /translation="MKIIEKKIANKKINWLVVNFLVLIAMIIFTSNIAHASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSAVNTIVIGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSIALI
                     LLMPTMISSGIQFAKTFYKDSTTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNNLTRKNFQQTDLTQVLTDKVIDKLEKENPIADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNSGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSNGFNRAKGSGE
                     GEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERATKPFKSMRDI
                     ANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSVPTRLSDRKDGAKVEN
                     ADRIMSSSERMREAMKNNAESNNNPASAIQQKVQQDIEERKNAMHGQAKSAEELINQK
                     RHEAKYTPEAMNREEIMLRKRVEGRTGANIDGKEALTKERIQEAKNSNTGLEKLVKEN
                     PQNSTSSHGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNAQGTKTIDVRENLQSS
                     STSQPKTVDVRENVQNSTSSQGFKSVDVRENVQGSSASQPKTVDVRENVQSSTSSQGF
                     KTVDVRENIQKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKSR
                     RRFTYEDNELFRDTLNDPNPLFDNLLRNKRGGLSCN"
     gene            3474..3671
                     /locus_tag="PW004_002508"
     CDS             3474..3671
                     /locus_tag="PW004_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727438.1"
                     /translation="MQLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKNEIESNFKCCSLEKNVRG"
     gene            3675..4286
                     /gene="mobC"
                     /locus_tag="PW004_002509"
     CDS             3675..4286
                     /gene="mobC"
                     /locus_tag="PW004_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HDL2727439.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVVLTKQLNQIGNNLNQAVKALNIIAKYFREESEDKKEK
                     SLHFSFDASRYNIFKDELANSIAKKLMISYPDDIFLESNRSAPSFFKLSEVEKEYFEY
                     WIEKQTRPGTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            4271..>4781
                     /locus_tag="PW004_002510"
     CDS             4271..>4781
                     /locus_tag="PW004_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HDL2727440.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDDSEE
                     ALQEMNLVRTKMNKSKDIAQAFHVIHSFDKSTSKELSIDKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNINMETCKRLRIDPKDLELMRKINDQVLNEHGLESVNNEY
                     YENLEKRTNL"
BASE COUNT         1839 a          653 c          940 g         1349 t
ORIGIN      
        1 tgaaaacaag agacaaaaac aattttgctg atagttcaaa aaatgaacta gaattatata
       61 agtcttataa agatagtaaa gatattaaag aatcacataa tgatgatctt cttgaatcac
      121 tttcaactaa aaataataaa ttagatgaag aattgatcga aacatttatt gaagaaaatc
      181 ttcttaaaca aatatgggga gaaagtattg tgaattgctt aaaactggct agcaatagtg
      241 attatcgtca attcgacaac tggtacaaaa aattcaaata tgcgattaga tctgctgaaa
      301 aagaacaaaa aattcaactg aaaattatct atgaaatttg taataacaag tattttgtag
      361 atcatgttag agaacagcta tcaatgactt tacgtgactt gatacgaaga gcaaaaacag
      421 atcatcgtat caaacaaaaa gacgattata tctttgcttc tctaaaaaat aaagcgttag
      481 aactaattga attgcaaatc agcgaaggaa tagataagca ataagaatag aattgagtca
      541 aatgaaatgg aggggtatct ccacttcatt tgagaacttt gaatatagga ggggttattt
      601 tgattaaaaa tcgtttaata gatcaagttt ggtgtctaga tcaaaataca gatagtctcg
      661 aattaatcga gactatttgc ttcaacacta tagttctcga tttaagagct gaaaatgata
      721 attgtgtaac tcatgtcata attaatcaaa aaatggctca gaagctctct gagagcctca
      781 gaaaatgggc taagggaggt aacgatgaaa ataattgaaa aaaagattgc caataaaaaa
      841 ataaattggt tggttgttaa ttttcttgtt ctgatagcta tgattatttt tacaagtaat
      901 attgctcatg cgagtatttt caattggttt gatgacacaa aaagtcaaac agagtttttg
      961 aataattctc tttatagacc ttacctaaaa aagtacaatg gagatttcct tagtcagttc
     1021 gggatttggc tcggatgggc tgtggtaaaa ggcatgttta ctgtgacaga ttcgattcaa
     1081 aatatgattc cagatgtgct ggatttgttc aatttcattg aaagtacagg gctaaataat
     1141 gtttatcaat cagccgtaaa tacaattgtc attggattaa tgatcctttc attgatgttt
     1201 gttggttaca aaatgattac tggaaaagga acagtggatt taaaatcggt tggaatgaac
     1261 gtagtgatgt ctatagcatt aatactttta atgccaacca tgatttctag tggtattcaa
     1321 tttgctaaaa cattttataa ggactctact acgattacaa attcagatga cggagtcgct
     1381 tggtcattga taaaacaagg agtaacagat ttagcatata ttaataaaac agatcagtac
     1441 tcaagcattg ataaaacgga ggatcgaaat aatttaacac gaaaaaattt tcaacagacg
     1501 gatctcacac aagtgttaac ggataaagtg attgataaat tggaaaaaga aaatccaatt
     1561 gcggacaatc ttcgttatga attagttgaa aattctaata atgaatttgt tgctacaaag
     1621 ttttctgaca acttcttatc aacattttct gatagcttga aatcaggcta ttaccgatac
     1681 caagcaaatc tatgggggat ttcaattggg ttgacagcgt tagcaatcgc ctatgtgttc
     1741 agcgcgtttg tgatcattac agcaatacta gaacttgcat ttaaacgtgt gttaggtgtt
     1801 cttgtttttg caacagatat tgagacagga cagcgttcaa aagttgttct atcagatatt
     1861 ctacaatgtt acttaactgt tgggtttcaa ggatttgggt tatcaatgtt tgctatgttt
     1921 atcaattttc tgaactcagg acaaggtatc tcaacaaata tttttataaa aacaattgct
     1981 tatatctgtg ccgtttttgt tttaatcaaa ggcagtggaa cagtcatgag atactttggt
     2041 gttgatattg gactcaaaga aggctatggg caacttgctt ctgcttttgg aatgggcgca
     2101 atgctgttta gaaaaggatc aaatggattt aatcgtgcta aaggaagcgg agaaggagaa
     2161 gatcgaaaac cagaaaagaa tttcggcgaa actctatcaa aaaaagctgg caaaactgga
     2221 agagctttgg gatatgccca tgaacgtgga ttatccggtt tagcttcaga tggagctaca
     2281 atggctagtg aaagagcaac aaaaccattt aagagtatgc gtgatatagc taatgatacc
     2341 aagaataagt ttaaagaagg tttagatgat ggtacagtgt ccgctattaa taaaaatagc
     2401 aaacctatgt tagctaaaaa taaagaagat gaaactggaa aatatgctga ttccgtgcca
     2461 acacgtcttt cagatagaaa agatggtgct aaagttgaaa atgccgatag aattatgtct
     2521 agttctgaac gtatgcgtga agcaatgaaa aataatgctg aatcgaataa taatcctgca
     2581 agcgcaattc agcaaaaagt ccaacaagat attgaagaac gtaaaaatgc tatgcacggt
     2641 caagctaagt ctgctgaaga attaattaat cagaagcgtc atgaagcgaa atacacgcct
     2701 gaagcaatga atcgcgagga aataatgtta aggaaacgtg tagaagggcg gacaggcgct
     2761 aatattgacg gaaaagaagc gttgaccaaa gaaagaattc aagaagctaa aaattcaaat
     2821 acaggattag aaaaattagt caaagaaaat ccacaaaatt ctacaagtag tcatggtggc
     2881 cgatcagtag atgtccgaga aaatattcaa ggttcactga atggtcatgt tggacggaat
     2941 gttgatgtaa gagagaattt acaaagttct tcaaatgctc aaggtactaa gacaattgat
     3001 gttcgagaaa atctgcaaag tagcagtaca agtcaaccaa aaacagtaga tgttcgagaa
     3061 aatgtacaaa attctacaag tagtcaaggg tttaaatcag tggatgtccg agagaatgtg
     3121 caaggtagca gtgcaagtca accaaaaaca gtagatgtcc gagaaaatgt acaaagttct
     3181 acaagtagtc aaggattcaa aacagtagat gttcgggaga acattcaaaa agatagtgga
     3241 gaaatgaaag aaaaaacaca aaaaatcaat atcgttgaag acagaaagtc agcgaaaaaa
     3301 tttgattcaa atcatgaaac agtgattatt gatagtgaaa tccgtgaaaa tgataaggga
     3361 tcaaaatcaa gacgaagatt cacttatgaa gacaatgagt tattcagaga tacacttaat
     3421 gatcctaatc cacttttcga taatttatta agaaataaaa gggggggatt gtcatgcaat
     3481 taattctaaa gattagtacg catgatttct tagaacttga cgaattatgt gaatcagtaa
     3541 agaacgcaac aaatgaaaaa atcacggtca ctaattatat tgaactgatg attcaagaag
     3601 aattaaagat gaaaaatgaa atagagagca atttcaaatg ttgctctcta gaaaaaaatg
     3661 tgaggggata atcaatggat gataaacaaa aaatgtacga aaaaaatcgc aaacatagtg
     3721 aggccttaaa ttttcgtgtc gataaaaaga cttatcttga actgacattg attgcaaaac
     3781 aaaggggaat ttctagagca gacgtttgcc gtgagcggtt aaagggactc aaatatgaac
     3841 cgccgttgtt agctgaagat gatagggtag ttttaaccaa acagttaaat caaattggta
     3901 acaatttgaa tcaagcggta aaggcattaa atatcattgc taaatatttt agagaagaat
     3961 ctgaagataa aaaagaaaag agcctacatt ttagttttga tgctagccgt tacaacattt
     4021 ttaaagatga acttgcgaat agcatagcaa aaaagttgat gattagttat ccagatgaca
     4081 tttttttgga gtccaatcgg agtgccccct ctttttttaa attatcagaa gtagaaaaag
     4141 aatatttcga atattggata gaaaagcaaa ctagaccggg tacgaaagaa gagtattccc
     4201 aaaaagttga tcggaattta attcactcaa ttcagattat tgcaagttta aaagatgagg
     4261 tgaaacaact ttgggagcta ctataacatc gaaaggacgc aagaaaaatt tatcctcgct
     4321 attacaatat gcagctaatc cttcaaaaaa agaaaaatgt gttgcagtct caggaataaa
     4381 ttgtagtgat gatagtgagg aagcactgca agaaatgaat ttagtacgta ccaagatgaa
     4441 taaatctaaa gatatagcac aagcgtttca tgttattcat tcgtttgata agagtacttc
     4501 taaagagtta agtattgata aaatgcacga aatagctgtc gaatttgcag agaaagcttt
     4561 tccaaattcg caaatcattg ttgcaagtca caatgataaa gatcattttc atagtcatct
     4621 tgtcattaat aatattaaca tggaaacatg taaaaggttg cgtattgacc ctaaagattt
     4681 agaactaatg cgaaaaatta acgatcaagt cctcaatgaa catggtcttg aatcagtgaa
     4741 taatgagtac tatgaaaact tagaaaagag aaccaatctt t
//
LOCUS       DANKKG010000156         4644 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000156 DANKKG010000000
VERSION     DANKKG010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.156"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(73..1221)
                     /gene="nagA"
                     /locus_tag="PW004_002511"
     CDS             complement(73..1221)
                     /gene="nagA"
                     /locus_tag="PW004_002511"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDL2727441.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1297..2133)
                     /locus_tag="PW004_002512"
     CDS             complement(1297..2133)
                     /locus_tag="PW004_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2727442.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2317..3534)
                     /locus_tag="PW004_002513"
     CDS             complement(2317..3534)
                     /locus_tag="PW004_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDL2727443.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3683..3862)
                     /locus_tag="PW004_002514"
     CDS             complement(3683..3862)
                     /locus_tag="PW004_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727444.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            3917..4000
                     /locus_tag="PW004_002515"
     tRNA            3917..4000
                     /locus_tag="PW004_002515"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3951..3953,aa:Leu,seq:gag)
     gene            complement(4240..4644)
                     /locus_tag="PW004_002516"
     CDS             complement(4240..4644)
                     /locus_tag="PW004_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727445.1"
                     /translation="MIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGI
                     YLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKE
                     LMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         1395 a          979 c          751 g         1519 t
ORIGIN      
        1 ttttactaga acacaaaaaa aaccttaaaa ccaaacttaa gaagtttagt tttaaggtta
       61 acttaaatta tattatgcgt ggaaacgttc cacgccatct agataagttg cttccaatgt
      121 catatcaggg ttcaatacga taaagtctgc atcgcgtcct tgagcgattt ttccacatgt
      181 atcatcgatt ttacagctga ttgctggaat cagactagcc atcatgactg cttgttctgg
      241 tgttgctaat ccccagtcta caacattttt aattgcttct ttcaatttca atatacttcc
      301 agctaagttg ccagattcta gacgtgctgt tccttctttt actactactg gaaattcgcc
      361 taaattgtaa tttccatcag gcatcccacc ggccatcata cagtctgtaa ttaatgcgac
      421 atgatcatgt cctgcttttt ccattaggat tcccgcagca tttggatgta catgatggcc
      481 atcacaaatc aattcagaaa agacatgttc taaagataat aacgcaccca ccatacctgg
      541 ttcgcgatga ttcaatccac gcataccatt gaaagcatgg acaaatacac ttgcgcctgc
      601 ttcaacagct ttttgcgctt cttctagtgt agcattactg tgtcctagag aaacaactac
      661 tccttcttca gtcacttgtt tgacaaactc ttctacacct tgacgttccg gtgctaaagc
      721 aatttttttg attaaaccac ctgaagcttc ttgccattca tggaaagtat ccaaatcagg
      781 atccccgaaa taacttgggt tttgtgcccc tttgtgttct tctgtgaaga aaggaccttc
      841 aaaatagatt cctttgattt tagctcctgg tgcttcctga tacattttgc cgattgtctc
      901 agctacatct tttaaacgtt ctttgcttga cgtcaatgtt gttggtaaaa atgaagtcac
      961 tccacaagat aacagacctt cagacatcac tttgatgcct tctgcatcat tatccattac
     1021 atcatgattc atatagccgt gaatatgtgt atctaccaaa ccaggagcaa tccatttgcc
     1081 gctttggtca atgatgttga cttctccctc aggtagttct ttggtgtatt caccaaaaag
     1141 tccatcggta atttcaagat agcctgcatg tttgacacct gacgctaaaa agaatttgtc
     1201 agcatagata aaagttctca ttctattctc tccctttgtt tttctacctc taaagtactc
     1261 ttatttagag aagaagtcaa gaatggtcta caccatttac aaattttttg aaatttgttt
     1321 cacagcttgt gctaagcctt catagaatgc tgctctttct ggttcttctt tacgcttgta
     1381 tattaagctt ttaagcacaa ataaatcatt gaggtaatag tagctattct tctcttgagc
     1441 ccaatagatt ccttgattca cctgttctaa cgcttcgtcg atttcatttt gatggaataa
     1501 gaaatttcca taattataaa aaatttgcga aaattcactt tttgatcgtt catcagctga
     1561 ttgatgcagc agttggatgc ttcgactcaa atagtagcgt gcttcagccg tttttcctgc
     1621 cacaccatat atttttccga tacaactaat cagttgtatt tctgtgtcag aaacatggat
     1681 tctttctttt tggctagagt agcttaatgc ttctttcaaa taataaaggg actgagagat
     1741 attttgacgc aacgtgaatt cgcaaacacc gagataataa taatagcgtt gaaagtcagt
     1801 tgcttgatac agattttcag aaatatcaga agattttagt acttgttcta acttccgata
     1861 atcacgtttt tggaaacaac tatccaacag ttcaaataat ttatcgtttg tccgagtagt
     1921 ttttcgagag agattcatca catgatcaat cgttacccct agccgttgac aaatctgcat
     1981 catcaccaat acattcggca gttcctcatt attttcgatc cgacttaata cactttgtga
     2041 acaaatatca tgcgaaagca ttttttgcgt cattttttgt tcttttcgaa tctctttaat
     2101 gatcgttcca aaggactcga ttaacgtttc cataagtatc ctccccaaaa tatgcgtatt
     2161 cgcattctat tcgtatttta ctttaggaag tcgcaaactt caagaaaaga cttgcaacta
     2221 aataatatac tttagttatt taagaaaact ccactagaaa gagagcctca gtgtgtttgt
     2281 ctgtggccct cctactgaaa aatttggttt ttaaaatcat ttttggcgat aggttgctag
     2341 gaattctctc attttcttag ctgtttgttt caaatcatcc attttaggaa gataaacgat
     2401 tcgaaaatga tctggctggt tccaattaaa tccaccacca tgtacaagta gaatatgatg
     2461 ttcatgtaaa aagtccaaaa cgaatttttc atcatccaaa atattgaatc gctttgtatc
     2521 aatttttggg aatatataaa aagcagcttt tggttttacg gctgaaagac caggaatatc
     2581 attgattgca ttatagatat attctctttg ttcatagatc cttccgcctg gcaggagcag
     2641 ctcatcgaca ctttgataac cgcctagcgc ggtttggata atttgctgag acaatacatt
     2701 ggaacacaaa cgcatggagg acaacatatt cagcccttct atatagcctt tgacgtgtga
     2761 cttatctcca cttaggacca tccagcctac tcgaaatcct gctacacgat gagatttgga
     2821 tagaccgttc agcgtaacaa cgaacaaatc aggcgctaat gtagcaatcg gaatatgttc
     2881 tagaccatcc atcactaagc gatcatagat ttcgtcggaa taaataatca ggttattttg
     2941 tcgagctact tcaacgattt cttctaataa ttcttttgga taaagtgccc cagtaggatt
     3001 attaggatta atgataacaa tagcttttgt ccgactcgtt atttttgatt tgatatcatc
     3061 aatatcagga taccattctg cctcttcatc gcaaatataa tgaacaggtg tgcctccagc
     3121 taatgcaata gaagccgtcc acaacggata gtccggcatt ggaaccagta cttcatctcc
     3181 attgtccagc aacccctgca tacacatcgt gatcaattcg ctcactccgt tgccggtgta
     3241 gatatcattg atcgtcacat tcggaaaatg tttcagttgg caatattgct caattgcttt
     3301 tctagcagaa aagatcccct tggaatctga gtagccttct gagtcacggg cattcatgat
     3361 caaatcacga atgacttcgt ttggtgcttc aaatccaaaa ggtgcgggat ttcccgtgtt
     3421 caattttaaa atactaatgc cctcttcttg catccgctcc gcttcttcta gaacgggacc
     3481 gcgaacatcg taactgacac cttctaattt gttggatttt tcaaattctc tcatcactta
     3541 gccaatccct catttcaaaa agttagaact ttccaaattt tttgttattt actatttcaa
     3601 tatcatactc catcctagaa aattttgcca cttatttagg aaaaacgtaa gtattaaaaa
     3661 aacacaaaca agcgttgaga acttaattgt catgatagtc attccataga ttcgctgccc
     3721 aatccattgc aattccacaa atgattccta gcataaaacc aagcaaaaaa cgtccgaatg
     3781 caagtccaat caatacacct aagatcattc ccagtcctga aaattctccg tcatatttct
     3841 ccatcttaat aagtcctccc atttttgagc ttgacaacta tgtaaaacct cgctatcatt
     3901 agtgtgttat tattacgcag atgtgttgga attggcagac aagcatgatt gagggtcatg
     3961 tgagcgttag ctcgtgtggg ttcgagtccc atcatctgca ctacaactcg tgaagttaat
     4021 tttgcttcac gagttttttt gtttgaacgt ttctttatgc cattatatac atatcactgt
     4081 atcctttaca aaaagaacgt acaatcatgt aaacagcctg aggtgagtat atgaaggtcg
     4141 gaaacaagct actcgtagaa acttgcaagt gttaaaacaa ttaatcgaaa aacgagtaaa
     4201 atccctcacc taatactaaa gcgagggatt ttttgtatat tatctgttta tcaccgttat
     4261 tataaaggta caaataccaa gcacggttaa tccaaattgc ataaattcat ttttttgtac
     4321 actcatgctt ttcattagct ctttaactgc cattagtact aagatggcga taacaacata
     4381 aagtatatag tacgtccgca ttccggaagc ccatactgtg ggagaaaatc ccataattaa
     4441 ccttgaacaa aatcccatta tcatcaaaat aactaaaaaa atagaagttt tcttattgtc
     4501 aaaacttaaa taaattccaa caattaaaca tagtaataat gccaagacta ctaaagtagc
     4561 tatccaagta cctggataaa aaagtgacag tttcgttcct aactctgtaa agagattatt
     4621 caggttacta ctactccaaa tcat
//
LOCUS       DANKKG010000157         4635 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000157 DANKKG010000000
VERSION     DANKKG010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.157"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            175..519
                     /locus_tag="PW004_002517"
     CDS             175..519
                     /locus_tag="PW004_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDL2727446.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            520..939
                     /locus_tag="PW004_002518"
     CDS             520..939
                     /locus_tag="PW004_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDL2727447.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1031..1381)
                     /locus_tag="PW004_002519"
     CDS             complement(1031..1381)
                     /locus_tag="PW004_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727448.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1347..2678)
                     /locus_tag="PW004_002520"
     CDS             complement(1347..2678)
                     /locus_tag="PW004_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HDL2727449.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2934..3500
                     /locus_tag="PW004_002521"
     CDS             2934..3500
                     /locus_tag="PW004_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2727450.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3645..3732
                     /locus_tag="PW004_002522"
     tRNA            3645..3732
                     /locus_tag="PW004_002522"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3677..3679,aa:Ser,seq:cga)
     gene            4066..>4635
                     /locus_tag="PW004_002523"
     CDS             4066..>4635
                     /locus_tag="PW004_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HDL2727451.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNG"
BASE COUNT         1685 a          778 c          891 g         1281 t
ORIGIN      
        1 aaaataaaaa aagcgttagc aactatgctt aaaaagcata tcgactgaat aaactaagca
       61 ttggacaggc tgaaagacta catttaaaat gaaatgcgaa gaaacaatag aaaataaagt
      121 tgccttagaa ccatagttga atcacctaac agtcgtgaag ggagaataag agaaatggca
      181 gaaaaacaaa ccgcaggacg agatcaatta ggagattttg caccaaaatt tgcagaattg
      241 aatgatgatg ttttatttgg acaagtatgg tccagagaag aattatctgc tcatcagcgt
      301 agtatcatca ccatatctgc attaatttca ggaggaaatt ttgaacaatt gccagctcat
      361 ctgaagattg gcaagcaaaa tggcattact agtgaagaaa tagcagaaat cattacccat
      421 ttagcttttt atgtaggatg gccaaaagct tggtcagctt ttagtttagc aaaagaaatc
      481 tatttagaag aaaatagcga agataagaaa gaggcgtaaa tgacgacaaa acacgaagaa
      541 atacacaatg gcgtgatttt tccagcaggt gaaaaaaacg aagcttacgc tcaagtattt
      601 accgggcaaa gctatctgaa tatgttagtg aatgatcccg aagtttctgt tggtgtaggt
      661 aatgtaattt ttgaaccagg ttgtcgcaat cactggcatg tccataaaga cggctatcaa
      721 atcttacttg tgaccggagg agaaggctgg tatcaggaag aaggaaagcc agcaagacga
      781 ttaatggctg gagatgtcgt cgtaacgcac aaagggatta aacattggca tggtgcgaca
      841 aaagacagtt ggtttgaaca tttggcgatc actgctggta attcagagtt tttagaacct
      901 gtaacagatg aagtgtatga aggattggaa aatcaataat gtcaagcttg aacagctcct
      961 gaaagtagat tttaatcgaa ctttcaggag ctgctttttt gattcagtct tttataaata
     1021 gaaaataggg ttaataatag gaatgagaag cacgtcgaaa gatcttaata ttgataatcg
     1081 gctgggcata catgatttcc acgtcatcaa tcaaactacc taacagatga taatcttccc
     1141 gatcatgtcg gggattacct aatagttctt gatagtagat ttccatacta gggtcccgag
     1201 gattgttcag caaatcagca agtacatcta aatcttctgg ctgagtttct gccgtaccct
     1261 gtatctctat gaaaagccct tcctcagcaa attctactcc aatacgtatt tcagttattt
     1321 catgggaata taaataagta agaagctcat cgatgattct ttttgataat tctttttcca
     1381 agtgtctttt tttcctttct gacagcggta aagttatcta gtatcggtac aaggatacct
     1441 gcaacgaaac cagttgaaaa accactgtta tatagattta gtcctccgtg caaagtgctg
     1501 acgttatgga caagagcaat atgtagtaca ccagctatta caccgtaaaa actgccgtaa
     1561 aaaccgctta tgggcgcgag acctgtccca aacacagctg caacaagcat gccagtactg
     1621 gtgatctcat gaaaagttcc aaacaaagaa gcaataaata ttccgactag tatcggaaaa
     1681 ctgtttttta aatgacaacc aaaagcactg aaacccaccg cagataaaat agcaccgatg
     1741 acaggtccat ttaactgacc gccaaccagc aaaacataag aagacagcat cagaccaact
     1801 aagcctatgt tgatcaaagt agcaccaata ccagaattag caatgaaatc agttgtcaat
     1861 tttccagatg aatcgaaaat ttctcgaatc cccaaaagcc gaaaggaatt ataatagaaa
     1921 ccaattccga ctgtcagcga aaaaaagcca aaaataatcc aaatgagagg aaagtgatat
     1981 tcattgaaaa ccaatgtatt ttcgacgatt gaatagctga ataacctcaa aacagcagta
     2041 aaagtcatcg caatcaaccc agaggtgaat ccaatattat aaagtgtgaa tccacgatgg
     2101 aaaactaaag tttgagcagc taagggcgga agaagtagac caattgctat gccagctaaa
     2161 ttccccaata aaatacctat cagtaaagga aaacgcatac caaaagtgat ataactaacg
     2221 accggtgata atgcgctacc aaacagtccg atcatgatgt actgagaaaa aggtttattg
     2281 acaaatacag cgtatagata cacacctaaa atgattgaga tagaattata aaggtttttg
     2341 ccaaaaaaag agaaaccggt aactgttaga atcgaagcaa ataagggacc gtttaaaatc
     2401 accttgtgcc gataagctaa aagcacagaa agtaaggtca aaatccctac atttatgaat
     2461 gcgcttccga aaccgcctaa agcaaaataa tctgtcaaaa gattgcttgg cgaggtaaga
     2521 atagttagca atccactcca attatcagta ggagtattag aaagcaagcc aataaaaaga
     2581 aaaaaaacac ctgtaaaacc aagataaaaa tactttcttt tctgtgaaac gattcgtttt
     2641 tgaggatgaa tataaataac gtcagtttcg ttttccatta cacaccttct ttttcatttt
     2701 ctttaactat aacttgaaat gaaagatttg gacaactatc atttaggtca aaactaaaaa
     2761 tgtaagggaa cccattgaat ttaaccaaat ttttttaatt ttgcaaaaaa aagttaaaga
     2821 agactgaaaa aaaatttaaa atataacgtt ttgagtaaaa tgtttgcaaa aaatgctaaa
     2881 aatagggtac aataaaacaa catatactgc agtaaagaag tgggggagtt ttaatgagaa
     2941 gaaaagtcta taccaaggat caaattctaa aagctgctta tgatgttgtt gcaaaggaag
     3001 ggtttaaagg atttactgcg agaaatattg ctaaaaagat ggggatttcg acacagccga
     3061 tttatctgga attcaaaaac atggaagatt taaaaaatac gttgctggat tctatctttg
     3121 aacaactaaa agaagatgtt ttccctattg aacgtactgg tgataaaatc attgatctcg
     3181 gtttgaacta tatctacttt gcaaaaaagc atcatagtct ttacatggca ttgtacattg
     3241 atgaacatgg ggacggaaag aaaatgcatg atttttcata caattatttc cgtgaaatag
     3301 ttaaagaaga tgaaagatac aatcaattat cggatgaata tgtcgatgct ctgcatactg
     3361 gtacatgggt agcagtgact ggcttagcgt cattgatgtc atcaaacatc atgcatccaa
     3421 cagaagaaca aatcattgca ctgatcaaac ggacaattga ctcgatttta gcattagata
     3481 atcctgaaat aatcagatag tgttctatca gtatgaattt gatcggtaag tccgttggct
     3541 gcaaactgtc aatggacttt tttgctaaaa aaagcgattt ctgttattta tctattagaa
     3601 ataggcttga catttcgtga aaaaagttta aaaatagtac ttacggaagg ttggcagagt
     3661 ggtaatgcac cggactcgaa atccggcgaa ccggctaata ccggcgcgca ggttcaaatc
     3721 ctgtaccttc cttatatcaa cgattagagg ttgtgaagaa gctgtcctgc atcaagtaat
     3781 aagaacggcc aaacaaaaat agcttttcct attttttcgt ttgattgggc ttattaccgc
     3841 agatgcaagc ttttgaacac cgtttgttcg gaataagggg ctgtgacaag acttttgtca
     3901 cagcccctta ttgttgtact tttaacctac aatatttttt gattccccct gagtactatt
     3961 ttttataaat gctagtaaat caatcttctt tggccttttt ttgataaaaa aaatactaga
     4021 tgtaagataa aagtgtaaaa acgatttgtt ttaggagggg aaacgatgtg taccgcaatc
     4081 acttatgcaa cgaaagacca ttattttgga aggaattttg attatgagat gtcttataat
     4141 gaagtggtca ctattactcc aagaaattat cgtttcgatt ttagaaaggt aaaaaattta
     4201 gataaacatt atgcaatgat aggaatagca gctggggtat ccaactatcc actctattat
     4261 gaggctacaa atgaaaaagg gttgagtatg gcaggactga attttcctgg caatgctgat
     4321 tataaagaat tacaagaagg aaaagataac gttgcaccat ttgagtttat tccatggata
     4381 ctagggcaat gctcaaccat agacgaagct aaagaattat tggctactat caatctagtc
     4441 aatatcgatt ttagtgaaaa actacccttg tcacctttac actggttatt agctgataaa
     4501 gaaaaatcga tagtaattga aagtatgaaa gatggacttc atttatatga taatcctgtc
     4561 ggtgtgttga caaataatcc gccatttgat tatcaattat ttaatctgaa caattatcgc
     4621 tcgctatcaa atgga
//
LOCUS       DANKKG010000158         4545 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000158 DANKKG010000000
VERSION     DANKKG010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4545
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.158"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            592..1827
                     /locus_tag="PW004_002524"
     CDS             592..1827
                     /locus_tag="PW004_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDL2727452.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(2826..3086)
                     /locus_tag="PW004_002525"
     CDS             complement(2826..3086)
                     /locus_tag="PW004_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDL2727453.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(3113..4414)
                     /locus_tag="PW004_002526"
     CDS             complement(3113..4414)
                     /locus_tag="PW004_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2727454.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
BASE COUNT         1577 a          922 c          701 g         1345 t
ORIGIN      
        1 taatgctcca tctactttaa tcaaaacaat ttccttccct ttaaaaaatt atttctcctt
       61 tatatcatac acaaaaaaaa gttctaaaca accaaaaatg tttagaacag aataaaaatt
      121 ttaattttaa aataatattc aaattaaaca actggcgtat ctgctccttg atttcttttt
      181 gtgttatctg aataattatc gtttacagtt ataacacata gtgaaaattg tcatggcgag
      241 aaagtctaag aatattttta aacataacta tttctttctt acgtgtgtat aatgacgaca
      301 gcacacctat caattaatat aattatttta tttttttatg ttctcatgtt tatttttaat
      361 gacacaactt ctcgtattta tctggcacaa catataaatc gatcaaaaaa ttgttgcgtg
      421 tttttttgaa aattggagtt tcaacttgat tacaaaattt aaatttagaa gttgtgaaaa
      481 cagcgagaga cagtttaata ttcaaaatat acataggcta ctcgaacgct gtgtttttta
      541 gtatactagt aagaaacgaa gggatagact cattccaata aggaggattt catgaagaca
      601 attctttttg ctcctgcagt attcaattta gctgagacaa cacgaatgat tgaggttgcc
      661 aaagctatga aagaccgtta ttactgtgta ttctttggct tttctaaaac ttttgctcct
      721 ttgatcacag aaagtggctt tgagtatcat ttactagcgc cgatccttac gaaaaaacaa
      781 gaagaacaaa tcataaaatt tgaccagttg cgtacactta aaaacccttt taccaaagaa
      841 atggttgcct ctcgtatcac tagtgaacag agtctgattg ctaagtttga gcctcgactg
      901 attgtgacag gttcgaatgt gacaattttt ttatcggccc gcaataaaaa gattccttta
      961 gtatatgtta aaccctatgc tttgagtgaa cctttttttc aatcagagca tgcgctgatg
     1021 ccccacgagt tacgtttctt cggcccttta agcaatcccc tttggcactg gacaaaaaag
     1081 cgtatagtaa atacccgatg gatccccaat gccttcaaac acgctgcaca aaaagaaatg
     1141 cttcctttac caaaaacaag tttacaaatg atggatgctg atctaaatct catcacaacg
     1201 cctaaactat ttttggaaga agaacagctg ccggaaaatt atcgaatcgt tggtccaatt
     1261 tttgctaagc tcagtacttc cttgcctgaa gaagtgctaa cttttataca gcgaaagcaa
     1321 caagaaagaa aacgtattat ctatttcgca atgggtagtt caggcaatcc gcgtatgtta
     1381 cgcaaaattt tgttcttctt acaaaaacaa ccggatctag cggttattgc acctattgac
     1441 tatctgcttg atgacattca atctgactca gaagatcttt taataacaga atatctgtct
     1501 agtcatcttt tgcatgaatt cattgatttt tcttttatcc acggcgggga agggaccgtc
     1561 caaactgcct gtgaatccgg caaaccgttt atcggtgtgg ggatgcaatt tgagcaaagc
     1621 tgtaatattg actattgtgt aaaatacgga aacgcaatcg ccctcacaaa accagtgact
     1681 gaaagaaaac tgcagattgc tctgcgcgaa gtttcttcac ccaagattcg tcagcaggca
     1741 cgcacgctgc aagccgtttt tccaataaat ggtccacaac aagcaattca agaaatcgaa
     1801 accttcctga gcaaccaccc aatatagttt caagtagaaa aagctttttt aatagagact
     1861 catcagtaat ttcgctattc gagacccaga cagtgaaaat acaaaaaaag taaatagtgg
     1921 attgaaaagt tagagttact taaattacgg tgtctaaaaa acacaaaatc atccttaaaa
     1981 taaaagatct gacgctatcc aaatgagcat aggtagcgtc tgccatttat tcttagtcaa
     2041 attctttgac acctttctct ggttggatca tctcaacgtt tcttcaaaag aaagaccata
     2101 ataattaggc aaatacattt tcttaaaatg tgtcatatag atctagaagc atcgctttgt
     2161 agagatttgt gccgctagtc ccctcaacaa ttactaaatc aaatgtagga tttaccactt
     2221 tatctttgat aatcaacctc cctcttctgt tgagacaatg atctcgcaat ccgatgcata
     2281 ctcctatata aactgccagc aatccccaca tattgagtag atcatgtcag aatgttctat
     2341 gtcgtcagca acgatacact aaaagtcacg ttcaccatga ctgattgcta ttcccagcac
     2401 aattatttct atacacgttt catgatttga ataaacactc acatcataca tattaccatt
     2461 catacaccat actgcagccc ctactatatg gttgtaagct cccaaatcta tatttgactt
     2521 gatcactttg gtagtagatc tactaattct aaatccattg ctgataaaat ttccattttt
     2581 tcctccttct ttctgccaaa acagttaaaa caataataat ctttatgtaa aaaagggctg
     2641 tgacaaaagc cttgtcacag tcccatattc cgaataaacg gtggtcaaaa gcttgcatct
     2701 gcggtaataa gttcaaccaa acgaaaaata agccaacatt tcacaaaaat ttaagaagca
     2761 attttcgtaa atttcttctt atttcttgaa gctaaccact tctgttacaa ccacttttag
     2821 aacatttaaa gcttcgatgt gacaaaccat ctatgctgct cgatcataaa attgatagca
     2881 aaaattccac aaagtacata tgtcccccat gaaaacaaaa cattcatatg cggtacaatg
     2941 atctttggaa gaatcacgat caaaaatgat gccagtgccc aaatccatcg attgatttga
     3001 atatgatatg tagctagcat attcaatata aggtgactag ccgttaataa aaagataaaa
     3061 acaaaaaata aaatcagcca atacattgtt tcccaccttc taaaaaccaa acttagacag
     3121 tctggacttc gttttctcca cgactttgtt catctgacag catcttttta tcataagctt
     3181 ttacaaatgg ataatagata ataaatgcta tcaatgtgtt gatcatgatc aagataacaa
     3241 cacgccagtc atttcctgct gaaagataac cgccgattgg agctggtaat gtccatgttg
     3301 gcaaaacagt aaatctattg accatttcta atttgactgc aaaataactg ataatagtca
     3361 aaactaaagg aactaagttg aacgggatga acatgtaagg attcaacatc ataggagctc
     3421 caaaaattat gggctcattg atattgaaga taccaggaat aatagaaaaa cgaccaacct
     3481 ctttcaagaa ttttgattta cagaagaata taaacaaaac agataccacg atcgttgctc
     3541 ctgcaccacc aattgttacc gtccattgat aaaactgttc aggagcgata tagggtaatt
     3601 gggtggcagc tgtaccatct gccaatgcct ttgcattctc atccaacatg atcagccaca
     3661 ttggacgaac aacagcccct acaatactca tgccatggat accaaatgac cacaacaaat
     3721 ggatcaaaaa cattggtagt aaaacaccaa gcaaattatt ccctaaaata ttggttaaag
     3781 gctcgaataa cgataataag gcagcgttta catcaaagcc cgcgacgctt cgaatcagcc
     3841 aagtaattgt taaaatgact gcacctggaa tcaatgcctc aaaactacgg gcaacagcag
     3901 gtggcacttg ttctggcatt ttgatagtaa tatttcgatt tttaaagaaa cgatatactt
     3961 ccactgcaaa aatcatagat aagatccccg caaacatccc agatgctccc aaagaactca
     4021 taggtaagac ccagccaaga ggtgcctctt caggagttaa aacgtttaca ggaacttgta
     4081 acataaagaa cgttcctaaa gataatatac cacctgtcac actatctaat ccatatgatt
     4141 tagctagatc cgaacccata acaaaagttg cataaatagc catcaatccc atcgtcatcc
     4201 taaagggtaa aacaatcgtt gccgaataag gggcaataaa ttcattccat ccaggaaacg
     4261 gtaaattgat aaagaccata aaaaatgtcc caatcaaaat caatgacaat gttgaaacaa
     4321 ccccattacg aattgctaat aaatgacgct gtgtaccaat ttttgccatt ggtgggacaa
     4381 gtttctcttc gacccatttc atcagctttt ccactaaaac cactcctttt caataaaata
     4441 tgaaccattc attgcatgac aacgcaaaga atgttcataa ctaattatat aattaagaaa
     4501 ttttatttca atatattctc ttaaataatc aaatttattt ttaag
//
LOCUS       DANKKG010000159         4343 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000159 DANKKG010000000
VERSION     DANKKG010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.160"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..2608
                     /locus_tag="PW004_002527"
     CDS             <1..2608
                     /locus_tag="PW004_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HDL2727455.1"
                     /translation="IPFLILALVYLSIGIYPGSSRSILASDAFSQFSNFHASFRNVLL
                     GKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFPNQMMPDALYVLTLLKVGCAGL
                     AFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIMWLDAFVYLPLVILGIHRVMDL
                     KRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLLTNWSEYKKTIVPYGITSLLAG
                     GASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDLIMKNMIGVYDTTKYGSIPFVY
                     AGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASFYLVPLNLFWHGMHAPNMFLFR
                     YAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAALFAVAWGIKPEGSYTYVTMTS
                     FVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMSAEALVNTNSMIHGILDDWNYA
                     SRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSANDSINYGYSGISLFSSIRNRN
                     SSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYNISKNESTFSKYGFRQAGQSGD
                     YTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNLINTLSGLNEQYFTFYTPTLKN
                     QLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQAYLSLFPTNFAQLESSSATIT
                     VNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGTKEVSFMEPKVVGLDVVAYQAA
                     MNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYDKGWKAKIDGKPVKVSNFKDAF
                     LMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFILYVWYLDRNRPFYGTIDPAIEE
                     KPRNTHRRRRRK"
     gene            complement(2699..3319)
                     /locus_tag="PW004_002528"
     CDS             complement(2699..3319)
                     /locus_tag="PW004_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2727456.1"
                     /translation="MFLINATDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKA
                     MHMVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPG
                     IEKRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTI
                     IKITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
     gene            3433..3972
                     /locus_tag="PW004_002529"
     CDS             3433..3972
                     /locus_tag="PW004_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727457.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANSYGYGGGMYFTFTGKRQYYSVKLAMQVY
                     NTFGVGSVSCTAGGLTIGK"
     gene            3969..4307
                     /locus_tag="PW004_002530"
     CDS             3969..4307
                     /locus_tag="PW004_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HDL2727458.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
BASE COUNT         1333 a          761 c          821 g         1428 t
ORIGIN      
        1 gattccgttt ttgattttag cacttgttta tctaagtatc gggatctatc ctggaagcag
       61 cagaagtatt ttggcaagtg atgccttttc acagttttct aattttcatg ccagcttccg
      121 aaatgttctt cttggaaagc aaagtatttt ttatacatgg aatgcttctc taggattgaa
      181 ttatttatct ttaatttctt actatctagg cgggatcttc acgccactgg tgttcttttt
      241 tccaaaccag atgatgccag atgcactgta tgttctgact ttgcttaagg tcggctgtgc
      301 cggattggct ttttggtttc ttgcacgtac gttcaagatt cctcgctggg ggcatgttgc
      361 acttagcgtc agttacgcat tgatctcatt tatcactgca cattcggaga tcatcatgtg
      421 gctggatgca tttgtttatc ttccattagt gattttgggg attcatcgtg ttatggatct
      481 taaacggccg aaacttttat ttatcagtta tttactcttg tttttatcca gtttttatat
      541 gggttttatg attggcgtat tttctttctt atattttatc gcccgtctat tgacaaactg
      601 gtcagaatat aaaaaaacga ttgttcctta tggtatcact tctttactgg caggcggcgc
      661 ttcaatgatc attgtattgc cagcagtgct ggatcttcgg tcaaatggcg aggcactgag
      721 tcagatcact acgttgaaaa cagaagcgac ttcttatctg gatctaatca tgaaaaatat
      781 gattggtgtt tatgacacga caaaatacgg atcgattccg ttcgtttacg caggtctgct
      841 accattagtc ctctgtctgt tttattttgc cagtcggaaa gtaccgttga aaaataaatt
      901 tttgtacggc agtcttttcg ccatacttat cgcaagtttc tatcttgttc cattgaattt
      961 gttctggcat ggtatgcatg caccgaatat gtttttattc cgttatgcct atctgttctc
     1021 tttcttagtg ctcctgcttg caggaaaagg atgggaaaag atagaacaag acgatcgtgg
     1081 gctgttgtta ggaatcattt tggtcgtagc tgctctcttt gcggtggcat ggggcatcaa
     1141 acctgaagga agctacacct atgtgacgat gacttccttt gtattgacag tggttttctt
     1201 agggatttat gcttttgctg tgggatttta tcagcataaa aaactttcat tcaatcatct
     1261 gagtatattg ttattattga tcatgtctgc tgaagcactg gtcaatacga acagtatgat
     1321 ccatggcatt cttgatgatt ggaattatgc ttcccgcagt ttatatactg atccttatcc
     1381 tgacttgaaa gctcttgtaa atgaaacaaa aaaagattcc gactcgtttt accgcttgga
     1441 aaatctggat ccagtatctg caaacgattc gatcaactat ggttacagtg gtatcagttt
     1501 gttttcttct ataagaaatc gtaattcttc ctcttacctg gataaattag gtttccgttc
     1561 aagagggaca aatttgaata tccgttataa caataatact ttgctaatgg atgggtttac
     1621 aggaatcaaa tataacattt ctaaaaacga aagcacgttc agtaaatatg gcttccgcca
     1681 agctggtcaa agtggcgact atacactgta tcagaatgtc aatgcattgc cactagcatt
     1741 tactgcaccg ttgtccgtta atgatgttga gcagccagtg acagataatt tgaccagtca
     1801 gacaaacttg atcaatacat tgtctggttt aaatgaacag tattttacat tctatacacc
     1861 tactttgaag aatcagttga atacgacgat cacaaagaca acgactggcg tcacttactc
     1921 agaaacagca gataaccagc caaaagagct cacttgggaa gtccatgtgc ctgcaaatac
     1981 acaagcttac ttaagtttgt tcccaaccaa ttttgcacaa ttagaaagtt ctagtgcgac
     2041 gatcactgtc aatggtacat cgcgaaaaac gcaaatcaac atttctggtc aatactatga
     2101 tcttggctat tatcctcaag agacaacagt gacatttact gcaagttttt atggaacgaa
     2161 agaagtaagt ttcatggagc ctaaagtcgt tggtctagat gttgtagcct atcaagccgc
     2221 aatgaatcag atcaaagaaa aaggtgtcga aatggaagcc actggcagaa aagcttctgg
     2281 gactgtaaat accgctgaag attccatgtt ggtgacaact attccttatg acaaaggatg
     2341 gaaagctaaa attgatggga aaccagtcaa ggtcagcaat ttcaaagacg cattcttgat
     2401 gataaaagtt ccagcaggta aacatactgt cactttcagt tacttgcctg aaggatttac
     2461 gataggtgca atcctttttg tcctatgtat tgcattgttt attctttatg tctggtacct
     2521 tgaccgcaat cgcccctttt atggcacgat tgatccagca atagaagaaa aaccgcgaaa
     2581 tacacacaga agaagacgaa gaaaataaag aaacgatgga atctgtgtca ctcttctatt
     2641 taaatggcaa aaatttcaat ctttaaatga caaaaaccac ctgatttttg gtggtttttt
     2701 atatacctat gactaaacta tctcttgctg cttcgacaac ttctacagtg atcttatcca
     2761 tttgattgat tcttaaaatc cgatcaatct gcgcaaataa acgatgaata agcctaaaat
     2821 ttccttttgt gatcttaatg atagttgtga tagcctcata atcagaaaaa tcttctagct
     2881 tgatatcaat tcctaaatct gcccatttgt attccaaaat atgatgtgtt tcgtccttac
     2941 taaggttatc aaattcatgt gcaaatccta tgcgagagta aagctgcgga taacgagaca
     3001 atcttttttc aataccaggc ataccgataa gaaccattgc cagatcattt ctatcgtaaa
     3061 tatctcttag ctgttctaaa tgctgaagct ttaaacgatc aatttcatcg acaataatta
     3121 agtcaattgc ttcataggtt ttaggcacag gaggatcttc ttgattgtat tttagtacca
     3181 tatgcattgc tttagtcaga cctattctac tagttattgt gttaatatcc gaactgacac
     3241 gagacatctt ttcagctggt acagtataaa aaactttatt tacacttaat atttcatctg
     3301 ttgcatttat caagaacaag aaaaagtctg aatattcaaa aaaaataacg gtgtacattt
     3361 ttaaaaggag atgtataatc gagatttaga aagcgttttc ctcaaaattt aatgaattag
     3421 gaagtgatta ctatgaaaaa attatttagc gcacttttta ttctgttatt tggctttatt
     3481 ggtattggga cttctggaaa tatagttaaa gctagcacga tggttgaaca aacaagtgaa
     3541 atggcaaaaa cttttagtca attaacacca gaagaacaaa gttacttcaa atccaaagga
     3601 tttagtgata aagatacttt ttacactcaa aagatagagc ctcaagatcc gaaaacacgt
     3661 ttttctgcaa atgtattgcg gttaaccgca tctactaaaa aaattactaa tacgcagggg
     3721 cggacagaat atattataac aagtcccaat tcggcgatgt atcgtatttc tacacgcttg
     3781 agtttaggta gtgcaaaatc atatactcaa acagtaaatc tagctaattc ttatggttat
     3841 ggtggaggaa tgtattttac atttactggg aaaagacaat attacagtgt aaaattagct
     3901 atgcaagttt ataatacatt tggtgtaggg tcagtctctt gcactgcggg aggattgaca
     3961 ataggaaaat gataatttct tactatgtct taaatagaag tgatgatgaa ggactcttcg
     4021 tttttcaatc attacatgag gatacgatgc taaaagagtt ttataatcgg attaagaata
     4081 aacagataga aacaaattta tattttttaa gtgactctat cttgcctttt attagcaacc
     4141 ctaaaattct taacttgttg cagacaaaag atttgaaaag tttaccgatt attgtcattg
     4201 atgaggaaat tgtaaaatat ggcgcttatc taagcgtaga agaattagaa aaattaacta
     4261 acattggaat gagtattcag gaggataatg gggtacatgg aacatagaat gaatatgcac
     4321 agttgagaac ggaatttgac att
//
LOCUS       DANKKG010000160         4340 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000160 DANKKG010000000
VERSION     DANKKG010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.161"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(118..876)
                     /locus_tag="PW004_002531"
     CDS             complement(118..876)
                     /locus_tag="PW004_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HDL2727459.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(894..1712)
                     /locus_tag="PW004_002532"
     CDS             complement(894..1712)
                     /locus_tag="PW004_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDL2727460.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(1709..2065)
                     /gene="rnpA"
                     /locus_tag="PW004_002533"
     CDS             complement(1709..2065)
                     /gene="rnpA"
                     /locus_tag="PW004_002533"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HDL2727461.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(2183..2317)
                     /gene="rpmH"
                     /locus_tag="PW004_002534"
     CDS             complement(2183..2317)
                     /gene="rpmH"
                     /locus_tag="PW004_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HDL2727462.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            2841..4175
                     /gene="dnaA"
                     /locus_tag="PW004_002535"
     CDS             2841..4175
                     /gene="dnaA"
                     /locus_tag="PW004_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HDL2727463.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDIKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
BASE COUNT         1286 a          880 c          767 g         1407 t
ORIGIN      
        1 ttttttctat caaagttagt gactacttca tcgtatactc tgataagaat gatttctagt
       61 aaaatatact tggtttgaaa aactttgtca acgtgattta ttcaaaaagt aatggaatca
      121 ataatatttt ttagcaggtt ctactactaa ataacgataa ggttcctcac cttccgaatg
      181 tgtttttaca tgttcatctt tgcttaatgc agcatggatt tgtttacgtt caaatgcagg
      241 cattggttct aagaaaacag gctgccctgt ccgttttact ttttcagccg tgcgatcagc
      301 taaacgctga atgatctctt ggcgcttttc acggtaattg cctacattga ctactacaga
      361 cagcttattt cttgctaaac gatggatgaa gacttgtgcc agatattgca atgcattcag
      421 tgtttttcca tgtttcccaa tcagtatgcc ttgcttttgt gtttctaaat ggaaaacaac
      481 catattattt gattctcttt ccattttgac aagagctgga gcatcaagct gttttgaaat
      541 ttcagtaaga taaactgcca attccgttaa agctgcttca tcacctagct cacttacttc
      601 ttctgttttt tcttcaagaa tcgtttcgtt gactacctcg atctcagctg ttggttcttc
      661 ttccaaaatt tcttctacag tctgttctac tgtttcagaa actttttcgc tgatacttgg
      721 ctggatcgaa agccgtgcat tctttcgacc cattcctagg aaaccttttt ttccttcttc
      781 cagtatttcg atttcgactt ggtctttggc caaacccaaa tcagccagac ctagttggac
      841 agcttcgtct attgttgcag cttcataaat cggcattagc attacctccc ctattatttc
      901 cgttttttct taggattcat tgctttctta agagcacgtt ctctttcgcg gattctccgt
      961 gcttcttctt cacgctcttt tcgaattttg aaagggttgt tgatcagcag cgtttgtaca
     1021 acttggaagg cattggaaac tacccagtat aatgaaagac cgctggctaa attgacaccc
     1081 atcactaaaa tcattacagg catcacgaaa ttcatgatct tcattgatgc attcgattca
     1141 atttgactca tgctagataa atacgtactt gcaaaagtaa agattgctgc taaaatcggc
     1201 aaaataaatg ttggatccgc gttccctaag ttcaaccata agaaatgccc ttgtttcaac
     1261 tctggtacac gtgaaataga ttgccataac gccatcaaga ttggcatttg tactaataaa
     1321 ggaagacaac cagcatatgg attgacgcca ttttctgcat ataaacgctg ctgctcttct
     1381 cttaacttac tttgtgtttc cggatctctt gaactatatt gttgctgcag tgcttttaat
     1441 ttcggttgta attcttgcgt tttacgcatg ctctttgttt ggaaatgcat caacggcaat
     1501 aggatcacgc ggatgatcag tgtaaataaa atgatcccta tccctgcatt accaaatgac
     1561 aacgccttga tcgcctcagc aaagtaataa acaatataac gatcccaaat tcctgtactc
     1621 tgtgcgctga cttccccagt tccgcaaccg gataagacga tgaccaaact aacgataccg
     1681 gccatcaaaa gtaaacgttt gtatttcttc acttactctt ttcctctctg tcgattattt
     1741 ttgcaagttt taagacgtgg actaaattgg agcgaacctc atcataagtc aaccccttca
     1801 cacttggtct agcaatcaca ataaaatcca actccggatc aatttcttct tttatagaat
     1861 aaactgctgc tcggattttt cgtttgactt cattgcggtt taccgcgttg ccgacctttt
     1921 ttcctacaga taaaccaact cgaaaatgag gctgtcccgg tttttccaaa cggtaaacga
     1981 cgaatttgcg gttagcaaaa gaatctcccg tatggaacac ggcttgaaac tctcgttctt
     2041 tttttactcg atacgctttt ctcattagtt ttctttcctt ttctatctct cacttacgct
     2101 cataatcata ccataattac gattgtcctt tcatcatttc cctaaaaaag agtataaaaa
     2161 aaaccactga aaccccagtg gcctaagctg aaagaacttt tcttcctttg cgacgacggc
     2221 tagctaacac acgacgtcca tttttagtac tcatacgttt acggaatccg tgaacttttt
     2281 gacgtttacg tttatttggt tggtatgttc ttttcatata ttccacctcc aaaatgttct
     2341 tatctatttt ataaacacag acatactttg atagtataac gacaaagtag tgattttgtc
     2401 aatataaaat agaagatcga ttgttaaaac ttatccacaa gattattttt ttctatggga
     2461 gtcctatgtg gaaaaagtta tgcacaggga ttatccacag ataataaatg gctggtttta
     2521 cacagaaaac acacgtgtaa aaaaagtaat ccacaatcag gtgatttcct gtgtattccg
     2581 tctcctaact attggtatat ctatattttt tatccacaga tgcaagagga aatctattga
     2641 tttttacgag ttttccacct gtgcattagt ggtgtggaaa actttttttt gacaactgtg
     2701 tttttctctt ttttcttttt gtcgattgtg tggataattg taattaaaaa atttttttct
     2761 tcggtttctg tggaaaacta ttgaaaaaaa tgctaaatta gtactagtta ttcacttttt
     2821 aatgaaggag gaggccttat atggtatccc tcgatgcttt atggaatgaa ttaaaagcaa
     2881 cataccaaaa ggatttatcg cctgctagct ataatacatg gatcgaaaca gcgaaccctc
     2941 gaacccttga tcaaaaccag ctcgttgttg aggttcctag caagattcat aaagaatatt
     3001 gggaaaaaaa cctggcgaca aaaatcgttg aagtcggtta tatgttatca ggaaatgaga
     3061 ttatccctcg ctttattact ggcgaagaag cagagcaaga agaagttata gaagaaaaaa
     3121 atccaaaagt tgtggcacct agtccgctaa aaaaagccat gctgaaccct aaatatacct
     3181 ttgatacgtt tgtcattggt aaaggaaacc agatggctca tgcagcagcg ctcgttgtag
     3241 cagaagatcc aggttctatt tataacccgc tcttctttta tggaggcgtg ggacttggta
     3301 agactcactt gatgcatgca attggtcatc aaatgctgca aagtcagcct aatgccaaag
     3361 taaaatatgt aagcagtgaa acttttgcta atgatttcat caattcgatc caaaacaaaa
     3421 cagcagaaga gttccgtcaa gaatatcgaa atgttgattt actgctagtg gatgacattc
     3481 aattttttgc agaaaaagaa gctacgcaag aagagttttt ccatactttc aatgccttgt
     3541 acaatgaagg gaaacaaatc gtactaacca gtgatcggtt gccaaacgag attccgaaac
     3601 tgcaagaacg cctcgtttct cgctttgcat gggggctgtc tgttgatatt acaccgccgg
     3661 atcttgaaac aagaacagcg attttacgta aaaaagcggc ggctgaacgt ctagagatcc
     3721 cagatgacac actaagctac atcgcaggac aaattgattc taacattcgt gaattagaag
     3781 gtgcgcttgt tcgagtgcaa gcttttgctg cgatgaatag tgaagatatt tcgactagtc
     3841 tagctgcaga cgcactgaaa acgttaaaat caggaaaagg acatcctcaa ttgtcaatcc
     3901 tgcagatcca agaagaagtg gcaaagtatt atcacatcca attgaaagat atcaaaggaa
     3961 agaaacgcgt caaatctatt gttgttccta gacaaatcgc tatgtatttg gcacgtgagc
     4021 tcacagataa ttctttaccg aaaatcggag ctgaattcgg tggcaaggat catacaacgg
     4081 ttatccatgc tcatgaaaaa atccagcagc tgatggattc tagtgtcagt atgcaaaatg
     4141 aagtatcaga aatcaaaaac tttttactaa attaatttgt cgattctatc aactgaaaat
     4201 agcatgtgga taaaagaaaa aaacgttcaa aagttatgca cactttttcc acagctgttt
     4261 tttcgtttct ctcttacgtt ttttgagttt tccacagaat caacatgccc tattactttt
     4321 attattcttt ttaactaata
//
LOCUS       DANKKG010000161         4290 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000161 DANKKG010000000
VERSION     DANKKG010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4290
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.162"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(60..2246)
                     /locus_tag="PW004_002536"
     CDS             complement(60..2246)
                     /locus_tag="PW004_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDL2727464.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            2464..3147
                     /locus_tag="PW004_002537"
     CDS             2464..3147
                     /locus_tag="PW004_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HDL2727465.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            complement(3228..3947)
                     /locus_tag="PW004_002538"
     CDS             complement(3228..3947)
                     /locus_tag="PW004_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDL2727466.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
BASE COUNT         1124 a          843 c          714 g         1609 t
ORIGIN      
        1 tttcgtacat tccttcttct ttcttgaaac tgaccacttc tgtcacaccc tctcttctat
       61 cactttcctt ttttcacacg actcccggtt tctttgataa atctcgatac tttcaatttt
      121 cttgaatttt tcatttgagg tacacataga tacagacgat ttcttgctct agtcatcgca
      181 acataaaata gtcttcgttc ttcttctatc gtcgttactg gttctgcttt tatttcatta
      241 aagtcgatta ctttcttttc tgtcgctccg ttataaggca aacttccttc gttacaacca
      301 atcaaaaaga catttcggta actaagacct ttacttttgt gaatgctctg gatacatagt
      361 gcatcaagtt ttccttgtct gtaagcaatc aagcgggttt ttaattcttt agctgtcgct
      421 tgggtttctt gcgataaggc ttggagtctt ctgaaagtag catgctgctt tacttcttct
      481 cgtatccatt cttgatcact ctggtcgatt tccttagcta tacgtggcag atgaagatag
      541 ctttgaacaa gcgaatagat ttcttgatgc tggtctgttt tacgcaacgc ttcctcaaac
      601 tctcggattt ctgcagattt cgattttaaa ttggggctta tcgacaaaat ataatctgca
      661 acggctatat tttgatattt aggagaaaag tattgatcgc attcactttt tttcaggtag
      721 cagtctgcca tcagctgccg gagcaattgc tggtaggcct gatttttctt ttgcagattc
      781 agtgctgttt gccaatgaac aattttttga tagaaatgtt ggaaagactt gataccaaag
      841 ataccttcat atggactatc tgcttccagt acaaaaggaa tattcgtttt cagcagagat
      901 tggacaagct gctgcgtttg tgaagctgat cggtataata tgattgattc cttatatttc
      961 ccattttttt gaataaatgt ctgaatttgt ttgattagcc actgactttc tgctttagta
     1021 tcagaaaaca aataggcttc cataacacct gcttcttttt gtgctggaat catcgccttt
     1081 ggtatccgat catttttatt atagctgatc aatctatttg ctccttggac aatagctgcc
     1141 cgagaacgat aattagttaa taagaaaaga gttttggtag gatattcttt ttcaaagttc
     1201 ttgatgtatg ctggctctga tcctctgaat ccatagatac tctgatcatc atctccaaca
     1261 acaatcaact tgatcctgtt ttcctttgtc atccgtttca cgatttccca ttgaagtgga
     1321 ttagtatctt gaaattcatc aatgaataga taatcatatt tttggccgat ataatgctgg
     1381 atctcctcat tctccaaagc gcttttcaga ttttggagta catcattgaa ctctagcaat
     1441 tgctggcttc ttttttgttc ttggagttcc gtgagcaatc catacagtcg ttctgatatc
     1501 ggctgtcgtg cattcttatc aatggaatca tatttttctt taactaactg aaccatttct
     1561 tctactgaaa ggttcatatt ctgccaatag tctaaccgag aagcaatcgt tttttctcca
     1621 tattgatcaa atatataaag attatattta cgtatcagct gttgcaaaac agcattcact
     1681 ttttgtgcag tatcctttga aaagtctcct tgtattcctt gcattgctac ttcaggaaat
     1741 tttcgacaaa tttcattgta tatcctaaaa aagatactat ggaatgttcc gatatggaca
     1801 gcggtttttc tctggctgaa attatctact cgttttttta gttcatttgc agcgttcctt
     1861 gtaaaagtga tcattagtat gcgttcttcg tctacttgtt tttgcgtcat cagataaaca
     1921 gcttttgcac agattgtttg cgttttgccg ctcccagctc ctgctatgat tcgaagatgt
     1981 ttcttcatat catatgtgat agctgctttt tgttcttgat tgaagcgatt ataggaacgt
     2041 ttctccatca ttttaaagaa agagctgttt ccttcacttt cttttacttg tacttcttcc
     2101 tcaataagtc cgggtgcttc gccataagcc gcttcatagg tagcaatgat ttcttcttgt
     2161 agccgaaaga cttgttgaag ctttttatac ttgttctccc agcgtttcaa ttctttttgt
     2221 aatcggagaa tttctttttc attcaactga ttgatttcct tcccatcttt cttctttgaa
     2281 tttgtttatt tcaagttggt tatttaaagt ttcgttgtct attttttctt tatactaaca
     2341 gacctttcta aaaatgaaaa tgggaagcga tatgatttta gttttctgaa aatttttcct
     2401 ttttttcaat cgtttatgct aagataagga agacaaaaat attcataaaa ggtgatgaaa
     2461 aaaatgcaga cttcctttca cgaggggcgt acaataggac ttttgctgac atttatcgga
     2521 ggagccatgg acagttatac ttatatccaa tatggtgctt ttgcctctgc tcaaactggc
     2581 aatatcattc tatcgatcat ccaagcattt gacggagaat ggctaagtgt tggtaaaaaa
     2641 gtattgtcta cccttttctt tttcttaggg attcttctaa caaagtatct aattgattat
     2701 tttagaaaaa aagaaaaaca tttctggcgc ttgtttgttc tatattatga agcagtgatt
     2761 tttttctttg ttagtttgac acccgtccat gagcatcccg cattagtaac gattttgatt
     2821 gcttttactg catcgatcca gtgggtagct tttgataaaa tcaacgggcg tgcctacact
     2881 aatttattta caactggaaa tttaaaaggg gtagcaacga acttatatga ttattgtatt
     2941 tctaaagaaa aagcagattt tgatcgattt ttccactatc taagtgtcgt tcttgccttt
     3001 atcgcaggag ctattgtcct tgttttctgt catcatctgt ttggcgccca ttcgattttg
     3061 atcgtttcca ttttattttt gatcatggcc ctcactcaga catttcattt atggcaattt
     3121 tatcatacga atcggttcac tatttgatct caatgaatca aaaaaactaa gcaagatgct
     3181 gctcacagag aaatatctgc gagtgagcaa tttgcttagt ttttatatta tttagttgta
     3241 aagattccct ttgctttcgg gttatttgta gtgtagcgga tcgactgggc atctgcctct
     3301 tctgtcattt tctttgcccg catcttgatt tcttgtacga tttgttctcc acatgccgga
     3361 tcggctacga aatcatattt gtccccatcg atcactattt tgggacatat atcaaactgt
     3421 tcataccact cttcgtatcg tcgattgagt tctttatagt aggaatagag ttcaggatct
     3481 tgttctagct gttcatattc ccggcctctt tttttgattc tcgctaacat agtttcaaag
     3541 gatacacgaa tataaaccag tagatctggt ttcttttgtg gcgaaatttc ttctaactcc
     3601 ttcagcatcg tttctaaaag accttcatac tgctttactt ctacttcaga aacacgccct
     3661 aaatctgcat tcaaatgaaa cagcatacta tcttcgtaga tggagcggtc tagtacgttg
     3721 tcatcatggc tgaaagcatc tttgattgcc aaaaagcgtt tgttcaaaaa gaaaatctgt
     3781 aataaaaaag tatacttttc tggattagaa taaaatagcg gcaatacttc attatcttcc
     3841 acgttctcat aaaaaggttt tgtttctaaa tcttgggcaa gcatttcagt tagtgtgctt
     3901 ttccctgcac cgatcgttcc tgcaacaatg atcacaatta tcggcatcct tcctgtaata
     3961 aaatctgttc ttcattttac tacttcattt atagaaataa aagccttgac tttatctgct
     4021 ccttaaaagc tacttctcta aaagtagcca aaagtaactc ctattcatga aaataaaggc
     4081 tgtgacaaaa gtcttgtcgc agcccttttt tcaaataaac agtattctcc tgccaacacc
     4141 tcggtcacaa ggcaatattc ataaaacatg aaaagctgtt ttttaacatt attccttgta
     4201 actcagagct aaacgggaga ctcacaacct ctttatgctc tcaacatttt atctgttttt
     4261 attcttctat tgtacttgat ttgcgtacaa
//
LOCUS       DANKKG010000162         4269 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000162 DANKKG010000000
VERSION     DANKKG010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.163"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(80..343)
                     /locus_tag="PW004_002539"
     CDS             complement(80..343)
                     /locus_tag="PW004_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HDL2727467.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(442..1737)
                     /gene="rho"
                     /locus_tag="PW004_002540"
     CDS             complement(442..1737)
                     /gene="rho"
                     /locus_tag="PW004_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HDL2727468.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(1734..3005)
                     /locus_tag="PW004_002541"
     CDS             complement(1734..3005)
                     /locus_tag="PW004_002541"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDL2727469.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(3171..4040)
                     /locus_tag="PW004_002542"
     CDS             complement(3171..4040)
                     /locus_tag="PW004_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HDL2727470.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
BASE COUNT         1145 a          920 c          767 g         1437 t
ORIGIN      
        1 tttaaattgg caaaaaacat aaaaaaacag actatcccta tcagatagtc tgctaaacgt
       61 tgagttatca gcgtttttat tattccgcag cgtttgcgtc tttgagaccg tattttttgt
      121 tgaaacggtc cacacgtccg tctgcttgtg tgaatttttg acgtccagtg tagaatggat
      181 gtgagtcaga agttacttcg acacggatga ctgggtatgt attaccgtct tcccattcaa
      241 ctgtttcttg tgaagttttt gttgaacctg acaagaattt gaaaccagtt gttgaatcca
      301 tgaatactac tgggtggtaa tctggatgga tgttttcttt catgattttt tcgctccttt
      361 gccctgctcc atttgctgaa ccagagttga tttgtcgatt tacaacatta acatcttaac
      421 atgtttcagt caaggttgca attatctttt cagatttctg gtcggatttt ttttaccaaa
      481 ggagacatct tgaaattctt tgaaaaattg ctgattgttt tttgttttac gcaaaaagcg
      541 gatcagttgt tccgtatatt ctaatgaatc tccagtcata ttttttcgaa gtttccaaat
      601 ttcttctaat tcttcacttg gcatcaataa ttcttctttt cgggtacctg attttttgat
      661 atcgatggct gggaagatcc gacgttcaga taattcacgt gaaagttgta attctagatt
      721 tccagttcct ttgaattctt catagatcac gtcatccata cggcttcctg tatcgactaa
      781 agcggtcgct aaaatcgtca aactgccacc ttcttcgatg tttcttgctg ccccgaaaaa
      841 tttcttaggt ttatacaatg cggctgggtc aatcccgcca ctcaatgtac gtccgcttgg
      901 tggaacgact aagttatagg cccgagcaag tctagtaata ctatccatca agataacgac
      961 atctcgttta tcttcgacta aacgcattgc gcgttccaat actaattcag aaactcgagt
     1021 atggttttgc ggctgcagat caaatgtaga agataccacg tcgcctttca cgcttcgttc
     1081 tagatccgtt acttcttccg gtcgttcatc aatcagtaga acgattaatt ctacttccgg
     1141 atagttttcg gtgataccat ttgcgatttc ttttaataca gaagttttcc ctgcttttgg
     1201 cggtgcgacg atcaatccac gctgaccaaa accgattgga gaaaagatat cgatcattcg
     1261 ggtagatagt ctttgcggtg ttgtttccag ttgaagctgt ctttgcggat aaagaggtgt
     1321 taaggctgga aaatgcggac gttcttttgc ttcttccgga tcttttccat taacgctttc
     1381 cacgtgcatc aaaccataat aacgttccga ttctttaggt ggacgggctt ttcctgccac
     1441 tttgtcccca tttctcaaac tgaatcgtct gatttgtgag gcagaaatat agatatcttc
     1501 tgcacttggt ccataattga ttggccgtaa aaaaccgtaa ccatcttgac caacgatatc
     1561 caatattcct tccatataat aaaatccttg tttctctgct tgtgcacgaa tcacagctag
     1621 agataattcc tttttattca tcttactgta ataaggaatt ttaaattctt tggcataact
     1681 ataaacttct tttaacgttt tgttctctag ttctgccatt gtcaaataat cgctcattca
     1741 gctcccactt cctctatcat ttcaattgat gcccctaagg ctgttagttt ctcgataata
     1801 tgatcataac cgcgtaagat ataatctaca ttgtaaatcg tcgttgtccc ttctgccaat
     1861 agtccagcaa tcactaaaca agcacctgcc cgaagatcag aagccacgac ttctgtgccg
     1921 tgaagtttat ttggaccatt cacgatgatc atatttcctt ccacggtaat atcagcgccc
     1981 attcgtgcta gttccggcac atggttcaca cgtttcgcat agatcgtatc gatgatttct
     2041 gaagtgcctg tagtcatcaa tagtaacgca gtaagtggct gctgcagatc tgtggcaaat
     2101 cctggataag gataggtcat gatgtttgcg ggttttaatt ctttcgaggg gtaaacttcg
     2161 atttcatctt cgctgatttt catatggaca ccaatttcct gcaattttgc tataaagctt
     2221 tccaaatgtt caaagatcac atttttcact ttgataccat ttcctactgc agctgctaat
     2281 gctagatagg ttcctgcttc gatgcggtca ggaatcatga aatgccgaca gccatgtaat
     2341 gtttcgactc cttcgatacg gatcacatct gtacccgctc ctcgaacttt tgcccccata
     2401 ttattcagta aagttgccac atctatgatt tccggttctc gtgcagcgtt ttcaattact
     2461 gtctgtcctt ttgcttttac agcagctaac atcacattga tcgttgctcc gactgaaacc
     2521 acgtccatga aaatccgatt gccacgcaat cctttatttt ctgtccgtaa atacatggca
     2581 ccatgttcat ttgtcacttc agcacctaga gcttcaaatc ctttgacgtg taggtcaatc
     2641 ggtctagggc ctaaataaca gccacctggc aggcctacta ccgcttcgcc aaatttacca
     2701 agcagtgttc ccataaaata ataagaagca cgcaaactat tgatttttcc gtgcggcatc
     2761 ggaatcgata cgacttctcg aggatcgatt tctagtatgt tgttggaaaa atggacagtt
     2821 gcgcccatga tctctaaaat ttcaattaat gaatgaacat cttgtatgtc cggcacacct
     2881 tccaagatca ctggggaatc agctaagatt gcagctggaa tcaatgcgac tgcactattt
     2941 tttgcaccgc tgatcgttac ttcaccagct aaaggtcggt ttcctttgat tacgattttt
     3001 ttcatctgtt tccaccttgt ttattaatct tttttcatgg atatgcttgt attttatcat
     3061 agataaaaaa aaagaaacaa gcatggtctg ccgttttaag taaaaaagaa tggagagcga
     3121 cttcaaaagt cgttctccat tcctatatat cgtatctgtt atgtggatgc ttacgctttg
     3181 ttagctgaac cgaaccagtc gatatgagat ttagcatctt caacgattgc tgctttacct
     3241 ggagctaata atttacgagg gtcgaatcct ttaccttctt tatctttacc agcttcgata
     3301 tatttacgag tagctgcagc aaatgataat tggaattcag tgttaacatt tacttttgag
     3361 atacccattg agatagcttt ttcgatttga tcttgaggaa tacctgatcc gccatgtaat
     3421 actaaaggca tgtctggacc tacagcatca gcaattgctt gtaagtggtc aaaagctaat
     3481 cctttccagt tttctggata ttgaccatgg atgttaccga taccgcaagc taagtagtcg
     3541 atacctgtag caaccatttg tttgcattct tcaatatcag ctaattcgcc gttaccgatg
     3601 attccgtctt cttctccgcc gattgaacca acttcacatt caactgagat accttttgca
     3661 tgtgcttttt caacaacgtc tttagctaat ttcaagtttt cttcaaatgg tagatgtgaa
     3721 ccatcgaaca ttactgaagt atagcctaat tcgatacatt ccaaagcagc ttcgtaatca
     3781 ccatggtcta agtggatagc aactggaaca gtgatgttca ttgcttcaac taaatcagtg
     3841 attaaatctt tacagatttt gtaaccaccc atgtatttag ctgcacccat tgatgtttgg
     3901 ataagtactg gtgcttgttt tgcttcagcc gcttcaagga ttgcttgagt ccattccaag
     3961 ttgtttgtgt tgaatccgcc gacagcgtaa ccgcctttac gagcagcttt taaaaattca
     4021 gctcctgata ctactggcat aataaaattc ctcctaagtt ttgtaaaact aaatttgtta
     4081 agacaattct taaccaacga ccctatttta ccaaacattt cggtagtttt ctactaaaat
     4141 caatgaaaat gaaaattttc aagaagtttt ttgcaaaaaa taaaatatcc aatagcgctt
     4201 taatcgaaaa aagtcttttg ctttctatct tttttcctac aaataataac aaatcagaaa
     4261 aagagaaaa
//
LOCUS       DANKKG010000163         4242 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000163 DANKKG010000000
VERSION     DANKKG010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.164"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(204..491)
                     /locus_tag="PW004_002543"
     CDS             complement(204..491)
                     /locus_tag="PW004_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HDL2727471.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(511..735)
                     /locus_tag="PW004_002544"
     CDS             complement(511..735)
                     /locus_tag="PW004_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HDL2727472.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(1190..1360)
                     /locus_tag="PW004_002545"
     CDS             complement(1190..1360)
                     /locus_tag="PW004_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727473.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(1375..1908)
                     /locus_tag="PW004_002546"
     CDS             complement(1375..1908)
                     /locus_tag="PW004_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HDL2727474.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(1929..2873)
                     /locus_tag="PW004_002547"
     CDS             complement(1929..2873)
                     /locus_tag="PW004_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HDL2727475.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
     gene            3510..3596
                     /locus_tag="PW004_002548"
     CDS             3510..3596
                     /locus_tag="PW004_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HDL2727476.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(3717..>4242)
                     /locus_tag="PW004_002549"
     CDS             complement(3717..>4242)
                     /locus_tag="PW004_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727477.1"
                     /translation="GLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEE
                     FTTCYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKD
                     TQTEEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNY
                     LKSVKEWLDQQQQN"
BASE COUNT         1177 a          764 c          654 g         1647 t
ORIGIN      
        1 gtaaagtttc gcttgtttca ccttcggtga aattgttgca aatcaataat taggtagagc
       61 tacatttttt tcgttgttca aagattctta tgccatttac tattaataaa tctataaata
      121 tgataatttt tcggctctat aatttatggg actgatgaat cagaattgat tcttcagtcc
      181 cattttcatt ttattccaaa gaattaactc caaataccaa tagaagccca tcctctatat
      241 acttgagatt ccttactaac aatttcttgt aattctatta aagatttagg taatccaaat
      301 ttatgtttta aaatatatct tccaagaaaa ctacttaatt tcatactaac ttcaccaaca
      361 ctttttccct cactgatttc tttagaataa agaaagagct tttcttttaa gtcaggatag
      421 tcatctatag ttgcttcatt gtatacttta ctaattgcat ttaaaagttt ttcttcttta
      481 gtaaaatcca ttataataac ctccctacca ctaccttcta ggtgcccatg gaccatgatt
      541 aacccaacca ttaacaataa tttttccaat ttctccttta gcttgattcc aatctaccca
      601 acatttttct ttgttacaat aaagaccatt tccatagtaa gtagctgcat caacttttat
      661 tcctgtaccg atgccagcta gacaagttcc taatattcct agaataacac aatgttttaa
      721 tactttcttt ttcatgattg tttttccttt tcgttttttt aaaattgctt gttatattaa
      781 ataatcttag taattttatt atataacatt ttattatata acaatgatta acgtcataaa
      841 aagtagtttt gaattcataa tcgtttaaat aaaattataa gatcagttat ttaaacagct
      901 agtagacata atgtcgacta gaccaagtag cccctctacc aatggataga gacgctcact
      961 ttgtgatttt tcattggtct aggtcaaaag ctttttttaa ggcttggttt tctgcggttg
     1021 tttctttcta attctttgtg aatattgatc ggtcattttt taaatacttg ttatttttgt
     1081 ttgtgtttgt tatgcttttt acatatctca tattcattta ttattctttt ctaaggttaa
     1141 taataggata atctctttca gaaagactgc tcgtacgagc agtcttttct tatttatcaa
     1201 gtttttctac aaatacttcc tcattttgac tgttgtagta attaggattg agttttagtt
     1261 cccctttttt atttcctttg ttggccactg ataataacat tgggctaaag cttaatattc
     1321 ctagtaggat agaaccgaat agcaagtgtt tttttgtcat aagtatttcc ttcttcattt
     1381 ccatagctgc caccattttt tccaattctt tgtttgttcg tccattgttt ctgttgcttc
     1441 agaaagagtc gttgaactat tctctgaaga ctcttccgta taagtcaact gcagttgctc
     1501 ctgaagtttt tcgatttgtt ggttggcttg cagggtgagt tgttgttgct ggtcgagtag
     1561 tttttgtata tgagaattat tttgctcttt gagggcaagt tgttctcgta aaaattcatt
     1621 ttcttcacgc aactgatcta gcaacttgtt tgcaaccacg tttttgtttg gtatataagc
     1681 atttttatcg ttgttttgtt catgtttatg caacttatct agcaaccttt gattgtctaa
     1741 gttgcgagca agttgcaata ctccagaacg caatacaacc ttataaccac gctcattttt
     1801 agctatcatg gaagaatcta atttttggat atgtttcgat attgcttgcc tactcatctt
     1861 aaaaagatct gacaattcct ttattgtaaa ggtttcttgt ggttgcatac tggttgcctc
     1921 ctaggttgct actggaataa attatctaaa tcagataata aacttttttt ctctttttcc
     1981 gaaagtgcgg actctgtttg tttttcaaga gcttttttct ctttttctag ttgtttgatt
     2041 tgttggagga gttcaagttc tcgtttcttc tgtttgattt tttcgttaat tgttgtagcg
     2101 ggttcttcgt attctttttg tttatcagct aaagccaatg ctttattttg gtcttctttg
     2161 gttggtgcat gtttgttaaa gtcgtctgct tttttgatct caggtttgaa cgtaaattga
     2221 tagccaataa caggttttcc ccgtcctttt ccgtatttct ttttgattgt tagtcctttg
     2281 aagattgcag ttagttcttg acgtataggt tttataactc gtttatctat atcaggttgc
     2341 ttataacttt taggaatatc taataattca agaaagtctt ctttggagaa atatgcgaaa
     2401 cctttggttc tgtattgctt taacagacga aacatagtct ttgcatagcc actttgtagc
     2461 tcgttgaatt gttgtaaaga atagcgtacc cactcgtcta aattatttaa caaaggcagt
     2521 gctttttcgt aaattcgtat atctacgtaa ggattttcta cgtccccgtt gattttaaat
     2581 tctgtaaaca ttacaaacat atcgtaagaa agcccagact cacttctacg accaaagcgt
     2641 aaagataaga ttttttcata agttgctttt aaatcttcta caaatcgtac atttgcagtt
     2701 gctttgtagt tacttaaatc ttttagttgg tcaaacgtaa aacgaactgt cttatcccct
     2761 ttttcacgca ttctagacac gatggaaaaa aacaaattca tttcaaccgg tgtaaatttt
     2821 cttaaaggaa ttgtatttaa ttcatggtgg tattttacaa tctcattagc catataatca
     2881 cctcaatcca attatgaatg acatttttta aatgtcaaga cattcatatt tgacgttcaa
     2941 acagacaaaa tgtccttcat aaacagacaa aatgtccttc ataaacagac aaaatgtcct
     3001 tcataaacag acaaaatgtc cttcataaca ttctgtatcc attggtagag taaggacaca
     3061 gcttacggta aagaggttta aaaagaggtt taaaaagaga tataaaaaga ggggggctaa
     3121 ttttcccgcc taaaaaccac ttcttttttc tttaaacagg ttcgcgcatt tttcaaaact
     3181 gccaatccaa aaccttgctt cctacggtcg ccaaaaaggt gtgatcgcta cgctctcaca
     3241 ttgtactgcg cagtcgccta aaggctcctt tgctaaaaac atgttggtaa agaggccttt
     3301 gagcggttct gaatgcttat ctaagccgtt ttaagacagt cttggataaa aatccttatc
     3361 tagctttaaa gcgccttaca ggcggtcagg gcaagcccta acaaccctaa aataagcgat
     3421 acttaaaaac accacacaag tttaattttt taagatataa taacttgtcg cctatccgac
     3481 agtcttgcga ctggaaggag gtgtatctaa tgtttgaatt gttcatttgc ccccttctta
     3541 tagaagtttg tgtctcttta ttcagttatt ggttgaataa ccgaaacaag aagtaaaatc
     3601 aataaaggcg acatcaacct gaaaaaaagc cccagagaat ccaacctctc tggggctttc
     3661 gtgtatctaa tgtttgatca tttgcatgtt tagtttaaca tgattgccga tcaaattcaa
     3721 ttttgctgtt gctgatctaa ccattccttg acacttttta gataatttaa tgccgtttct
     3781 tttttcgttg gattttgtat gttcgttgct tgctgaagca gtgctttata acgttcgtag
     3841 gtaactgatt gttgccgttc ttttcgttcc tgttcactag ttgaaaaagg gattgtatca
     3901 ctgaataaat tataacaagc ttcttctgtc tgcgtatctt taaaatattg cacgtctttt
     3961 ttggttcgct catagttgtc ttgaacctct ttttgctgtt tttctggcaa gttttgataa
     4021 agacgagtga gtttttccat gtttttatcc gtcaaacgtt tttcttgatt cacaaaaata
     4081 tcgttcattt cctcataaca agtggtaaac tcttccattt tttgtgttaa cacttgctcc
     4141 tttttctttt tatcacatgc tgcttcatac ttttgagtga tatcccctaa taaaatactt
     4201 cttgaaatac gtttatttcc ctctaaaacg ccgaataaac ca
//
LOCUS       DANKKG010000164         4238 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000164 DANKKG010000000
VERSION     DANKKG010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4238
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.165"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(1014..1304)
                     /locus_tag="PW004_002550"
     CDS             complement(1014..1304)
                     /locus_tag="PW004_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727478.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1706..1942
                     /locus_tag="PW004_002551"
     CDS             1706..1942
                     /locus_tag="PW004_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727479.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1949..3901
                     /locus_tag="PW004_002552"
     CDS             1949..3901
                     /locus_tag="PW004_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HDL2727480.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3973..>4238)
                     /locus_tag="PW004_002553"
     CDS             complement(3973..>4238)
                     /locus_tag="PW004_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDL2727481.1"
                     /translation="SIEDVFELCKNEEEIFIFGGEQIYNLFFPYVEKMYITKIHHEFE
                     GDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYERKNLLS"
BASE COUNT         1468 a          725 c          719 g         1326 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tatagattta agtagttcag ttttggagta caaaaagtga
     1501 aaatgtaaac ctaaatgagt atcaaattgc aactgaattt gatactcgtt ttttattgtt
     1561 aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag ttaaaatgta tataaaaatg
     1621 cgaacgtgaa ataattattg taggaatgca aattggtgtg ttaaaatggt tacaatttac
     1681 taagcaaaaa ggggtgcatt ttcctatgca atttcttgag ccaaaaaata agaacgctaa
     1741 gtctgtggat tgggaaatat cagagcaagt aagggtgata gttaagcaat acgctgaata
     1801 tgcagagcgt actgaaagcg aagcagtgga tgaattctta ttgaacattt tagacgataa
     1861 gaagtttatt gaatggattg ccaacaagcg aagtaataag cggattgttg aaaaaatggg
     1921 tatcaaagat agagtgggtt gatagacaat gaccaagctt aaaagattag ccacgaagga
     1981 agatgaaatt gtagatgtta aaattccgat ttcaaacgaa gaattaaaag atagggctaa
     2041 acaatatgat ttattaactc caaaacgatt tgcaacaagg tataacaaaa tgttgttctt
     2101 gccaacgtct tttaaatgga acggttctga atatcccatt caatataatt actgcatcaa
     2161 tccgttttgt tgtaattttg ggaaagaaca gcacaaattc aaagatgtaa aagggaaacc
     2221 aagtcgatat aaaatgactg gtagttccaa agataaaggt cataaaggta tgtactgcaa
     2281 tgacaatcct attggcagag gtgtatcgca aaactgtaca gtaacccctt tatctaattg
     2341 gtctgttgtt gaagaaatta aacgccttat agaaatcaat agcatccaag atgttgagcc
     2401 agattatcaa tttcataaag aaggctgctc ggaagaagaa tcaaccccat tcaatgagcc
     2461 gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct caacgctatc aatgtaaggc
     2521 gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa accactactt atcaccaaca
     2581 aaagaacacc attcttccaa tgttcgctaa gatggttgtt ggaagggttt cagtcagtcg
     2641 tacttgtgat attttaggaa ttggagttgg aacctactat cataagctgg agtggctgta
     2701 taggcgttgt ttggagtttt tagaacgata tgagacacaa ccccttcaaa caaagaaatt
     2761 taacgagatg tggcttaata cggacaagat gcactactac ctaaataacg tccgtaagaa
     2821 aggacaaggt tcaaagaaat atacggggtt tgaagacctt aatatgcaaa cgtatatagt
     2881 tgtatctgct gaggtcttat caagatatgt gttccgttct gatgttgcct atgattggaa
     2941 tatttcaatg gatgaattaa atgaagatac aagaaaattc aaagaagacc atctcaatac
     3001 ttttagtcgt aaaaatgata ggttagattg gtcttattat ccacaagaac catccgctaa
     3061 cgatagcgaa aataggaatg cttatcttca cgaattaggg aaaattacta ataggagtag
     3121 atttgtcgat ggtttaaatg ttgatgctcc ttatactaca actgctcact attggttaat
     3181 taaacaaatg gttaatgccg acgaatggcg aatgattagt gatgatgact tctctattag
     3241 aaacgccttt tatagagtat tcacgaaaga attaaggttg agcgatgcac atcattttat
     3301 ttgccaagtt aacaagacga aaagcagaaa acaatgctta aaagaattcg gacaagcaaa
     3361 agcagaactg ctcgattggg gagatatcag aggattcaaa acaaagtttc tacggacttt
     3421 ggcatctcat tatctaactg aattactaac aagtcatcaa ttccacgaag aggctattag
     3481 taaagatgga gaaagatatc gcaaatatgc cgataaccct atcaaacatc cattagccac
     3541 taaggataaa ggattttatt cagttgattg tagaacggat ttatctgctc tggaaccaaa
     3601 cgaaatcgca aagatgctat taaatgttaa tgaccattcg accaatagct ttattcaaca
     3661 gataagacgt tatatatcat ctcttgaaag accattaaca acagctcgtg gtgataagaa
     3721 gagctatatt tacgctaact tcaatccgaa gtatgctcaa tttgcgatta caattctaag
     3781 aacttactac aacttttgtc gcccttttaa atcagcggat aaaaaggttt tgacacctgc
     3841 tcaaagatta gggattacag ataaacaatt tgattggaaa gatataattt atttcaaatg
     3901 acaaaaagag cttggagtaa tcccaagctc ttgtgcttta tctttactgt tcttcttcaa
     3961 ttaacgcacc cgttaactca ataagttttt tctttcatat acatgaaaat agtagttata
     4021 cggattttta tcattcttta tccctttttg ggcaaatacc tcattccatt cctcataatt
     4081 cacttctgga aaaaaagtat ctccttcgaa ttcatgatgt atttttgtga tgtacatttt
     4141 ctcaacataa gggaaaaaca aattataaat ctgttctcct ccgaaaataa aaatttcttc
     4201 ttcgttttta cataactcaa aaacatcttc tattgaat
//
LOCUS       DANKKG010000165         4223 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000165 DANKKG010000000
VERSION     DANKKG010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4223
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.166"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            19..1353
                     /locus_tag="PW004_002554"
     CDS             19..1353
                     /locus_tag="PW004_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2727482.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            1394..2305
                     /locus_tag="PW004_002555"
     CDS             1394..2305
                     /locus_tag="PW004_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2727483.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            2302..3156
                     /locus_tag="PW004_002556"
     CDS             2302..3156
                     /locus_tag="PW004_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2727484.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            3153..3770
                     /locus_tag="PW004_002557"
     CDS             3153..3770
                     /locus_tag="PW004_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HDL2727485.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            3772..>4223
                     /locus_tag="PW004_002558"
     CDS             3772..>4223
                     /locus_tag="PW004_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDL2727486.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYP"
BASE COUNT         1270 a          730 c          856 g         1367 t
ORIGIN      
        1 aaaatttgga ggtaaaatat gaaaaaatta ttgtttacag ggttgctggc aagttcagca
       61 ttgtttttat taggaggatg cggatcaagt aatgcaagcg atgccagttc aagtgcagca
      121 aattcttctg aaaaaacaag tgatggaaga aaaaatattg atttctggtc cttttgggga
      181 tccggtgcac gaaaagaagc tatcgaagaa atcattgagg atttcaatca atcacaaaac
      241 gagatccatg taaattataa gtatcagcca tggggtgaca tctggactaa atctttatct
      301 gcaataacag ctggaaatgc acccgatgtt attgtacagg acataaattc cgttgcccaa
      361 agagcagaag cacagcaagc aactaatctc tccaaatatg tagaaaaaga cacttctaag
      421 gatttttatc cgcagctttg ggatacagta gaatatgaag gtgatcctta tgcgatccca
      481 ttcaatacag atacacaagt cattttttat aataaaaagt tatttaaaga agctggaatc
      541 agtgaacaag atctaccgac aacttgggat gaattagaag aaacagcacg taaactagat
      601 aaaaaagaag gaaaagaatt tacacgcatc ggtttttatc cattatggaa tttaggggct
      661 gatgtatggg cgctaaacgc agataacggg actagttggt ttgacaaaga tgaaaaagtc
      721 aagattgata ctaaaaataa agtggaagcc ttggagtgga tccaagactg gcaagattat
      781 tatgggaaaa atacgatcaa taaattagaa gccgaatttg ggtctggtgt atctgatcca
      841 tttatttccg gattagtagc catgcgtgcc caaaatatca attattactc tagtttgatg
      901 gaaaatgcac cggatgattt tgattttggc gtgatgcagc tcccagaaaa agaagaaggt
      961 tcaggccatt ggtcgtgggg tggcggattc gtcttggaag taccatacgg agcaaaagat
     1021 cctgaggcct cttatgaatt catcaaatat ctttcttctc cagaagtaca agaaaaattc
     1081 ggcgaaaaaa gttttgatat catggcgagc aagacagcca atgagaattt agtgaataat
     1141 gacaacctaa cggataaagg aaaaatgatc tatcaaatgg cggacgaaaa ctttaaaaat
     1201 acggtgatca caccagttcc tttagatgct ccggattaca cgacattagt gaatgaacag
     1261 attgaccaga ttcttttagg aacaaaatca ccaaaagaag gattggcaga tgcacaaaaa
     1321 gcagtagaaa acttagtcaa gcaaaatcaa taatccattt ttgactgtct aggtacagag
     1381 gaggcaagaa aatttgaagc gtttatctat ccgaaagaaa aaagaagcaa gatgggccta
     1441 tttatttatc tcgcctttta ttatcgggtt cttggtattt atgctagggc ctatgctttt
     1501 ttcagttatt ggtagtttca ccgattataa tttgacatcc aagatgaatt tcattggtct
     1561 agcaaatttc aaaagaatgt tcgttcacga tgatttattt tggaaatcgt tatacaacac
     1621 cgtttatttc gtcatcttca atgtaccgct gacgacgatt ttcagtgtgt ttttagccac
     1681 agtactcaat caaaaaatca aaggaatcag tgtattccgt acagctttct atttaccggc
     1741 agtcttatca ggagtagcag tctacatctt atggatgcag ctgctttctc catctgcagg
     1801 attgatcaac acatttctgg gctggttcca tatccctgga ccagcttggc tgtttgatcc
     1861 acaatggacg aaacctgcgt tgattttgat gaaaatatgg agttctggag gagcaatgct
     1921 actttactta gctacattac aaaatgttcc tagatcttta tatgaatcag cagaaatcga
     1981 tggagcaggc gtttggggaa agttcaaaag tattactttg ccattaatca ctcctattat
     2041 tttttacgat gtgatcacct ctacgatcgg ttctttccaa atttttcaag aagcttatgt
     2101 catgacgaaa aatggaacag gcggacctgc aaattctttg cttttttata atctgcatat
     2161 gtggaacaag gcgttcgtag cttttgatat gggttatgcg atggctatgt caatgatcct
     2221 gtttctgatc gtgttggttc tgacgtttgt caatttgaag cttgcaccta agtgggtcta
     2281 ttattctgga ggtgacggca aatgatggat tatttcaaaa agaccagtgt gaatcggcaa
     2341 acaaaaaaaa tactaagtta tgtcctcatg acagtaatag gaatcatttt gatcattcca
     2401 ttgctttgga tggtgtttac ttcattgaaa ccaatggaag aaatcgttcg ttatcctccg
     2461 acctttttcc cagaaaaaat cgtatgggaa aactatctgg atacgattgc cgcctttcct
     2521 ttttggcgct atgcaagaaa tacattattc atcactgtgc tagtagtgat cggtaatgtc
     2581 ctttcaaatt cttttattgc ttatggattt gcgaagcttg attttcctgg gaaaaagctg
     2641 atgtttgctt tggttctttc tacgatgatg atcccggggt ttgtaacgat gatccctcaa
     2701 tatgtattat tctccaagat cggctgggta ggtacttatt tgcccctgat cgttccatcg
     2761 ttcttcggca atgcgttcaa tatatttctg atgcgtcaat tttacttatc gatcaataat
     2821 gaattgatcg aagctgctga aatcgacgga gcgaatcatt tgtatatttg gagtcatttg
     2881 atgcttccat tgacgaaacc tgccttgatt accattgcga tcaattcctt caacgcagct
     2941 tggaatgatt tcttaggtcc tctgttatat atccaagatc aagaaaaata cacgttacag
     3001 attggcttgc aagtttttca gaaccaagca acgacacaat ggaattatct gatggcagga
     3061 gcaacacttg tcttgatccc gacgattttg ctgttctttt ttgcacaacg ttatttcatt
     3121 gaagggatgg atttgacagg aggttcaaaa ggatgatgaa tcgatcgatt ggtaaaaaag
     3181 ccaatgatta tttcttagaa atcattgaat ggattccacg tcttatccat ttacagctac
     3241 tatggttcct gtgcgtcttg cctgttttca cattaggaac tgcaagtcga acagttctgt
     3301 atatgattta tcattacaaa aaaaatgaag agaaaaagct cggttcacta ttctggaaaa
     3361 agtttcgtga gaacttctct ctttatggaa agcaagatag tttagcaagt gtttattttc
     3421 ttcttctatt gatcgattat cagattttcc gctattgggg tgggggaatt ggctatacat
     3481 tgatgtatac tacgttgttt ttgcttctat taagcgcgct tctctttagt tacaaaatct
     3541 tgcttcaact ggaacaaaaa gaagcttctt ggattactgc atttttcttg ttttttaatg
     3601 atttcaaaag cgcgcttttc tatttggcag gcacgttgat ccttcttgtc gcattctggt
     3661 ttgcaggacc aatctatttt gctttattag gtttcagttt cttgttttat tttcaagtcc
     3721 tgctttttat ccatcgtacg caagaaaagg ttcttaaaaa ggaggaataa catgttgcgt
     3781 ttgattgcta aaatcgatga tagtattatc aacaagttcg ccaagcaacg gaaaacggat
     3841 agtcgaaaaa tcaatttttt gaaatttatc agtaatatct ctaatggtgg ttttatttgg
     3901 ttgctccttg ctgctgtttt agtcttgata cctgatacta gagaatatgg tctagccatt
     3961 cttatcggca gtttgatcta tttttttgtt ggtttgcttt ttatcaaaaa aacgacaaga
     4021 cggaaaagac cttatgaaaa aaatcgtcgg cttgtccttt acacaagtaa accgaagact
     4081 tcttcttttc cttcaggaca tacagcgcta gcctttttat cagtagctgc gttgttcgaa
     4141 ttaagtccag ttgcaggaat attagccttt ttatttgctt gtttgatcgc ttattcgagg
     4201 atttttttac ttttacatta tcc
//
LOCUS       DANKKG010000166         4220 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000166 DANKKG010000000
VERSION     DANKKG010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.167"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(<1..641)
                     /locus_tag="PW004_002559"
     CDS             complement(<1..641)
                     /locus_tag="PW004_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDL2727487.1"
                     /translation="MNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQ"
     gene            complement(628..990)
                     /locus_tag="PW004_002560"
     CDS             complement(628..990)
                     /locus_tag="PW004_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727488.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFLRTSWQLEYS"
     gene            1188..2429
                     /gene="sstT"
                     /locus_tag="PW004_002561"
     CDS             1188..2429
                     /gene="sstT"
                     /locus_tag="PW004_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HDL2727489.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            2576..3211
                     /locus_tag="PW004_002562"
     CDS             2576..3211
                     /locus_tag="PW004_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HDL2727490.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            3402..4142
                     /gene="truA"
                     /locus_tag="PW004_002563"
     CDS             3402..4142
                     /gene="truA"
                     /locus_tag="PW004_002563"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDL2727491.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
BASE COUNT         1343 a          658 c          844 g         1375 t
ORIGIN      
        1 aactgcccga tatttatcag ccatgccttc cctgagtttg gaaattcacg tttcataaat
       61 gtagatacgc gataagaaac taaagggaaa ttacatataa cttgagtgac aatcaaaaat
      121 acagcaagaa aatttcctct tccttttata cctaatttat cagatagcat ttctaaaaat
      181 aaaaacgaag agaaagtata atacattgcg aagagagaat atggtttaga aaacctcagc
      241 gctgtatctg tatttcctct gatgatgagt gaaatttgtg tcatcaaaat aatcaaaccg
      301 aataaaacaa tcaagctgat aaatacttga ggaagcttat agcggacctt ttctttccag
      361 ttccattctt tcaaaccata acccattaaa aataaataaa aaatccataa aatactctgt
      421 ccattctgta atgcaaacgg gtctttatta aatagagtag agcagccaac taatccaatt
      481 gttaaaaata atagtagata tttaattgtt gttttcgata atttatttaa aaatccaact
      541 gtataaggaa tactacaaaa aagcaaagta caagaaacag cataagtaaa gtaattctga
      601 ctgataggtg ttaggataag ccaataatca cgaatattca agctgccagc ttgtacgtaa
      661 aaaaaagata ttgcaaacta gtgtggctat taggaatata aaggcaaaac gacttaaata
      721 atgtaaaact tttaaacggg cgattttttg atattggtaa cccatgtaaa gtgggatcaa
      781 atttgcacca ataacaacgg aagtttgtaa aaatgtatag atgtagaatc gaccagggtt
      841 tgttgcatat gtaggaatag tgactgctga aatcaatata cgctgtaaaa ccatcattat
      901 acaagcgaca aagattgtca ggaagccata actctccttc tttatttttt tcaagaataa
      961 acacctcaca aaaaaattgg acaaaaacat aagaaaacgt ttcctgataa gttgcccatt
     1021 aataacatcc taacttagaa tatctcaaaa aacaacgtgt agacagaaaa agtacaaaat
     1081 tagcaagaaa tggttgtttg tattgttaga atttttagaa aattatacct ttagtttctt
     1141 tgcataaaat gatacaatga ttgttaaaat tgtgaaaggg tggtcatttg ttagagaagt
     1201 ttttggatat gtcgttagtt caacgtattg ttgttggtat tttagtcgga atattgtttg
     1261 gtatcttttt ccccacatgg acatttattt caatattggg aacgttgttt gtcagttgct
     1321 tgaaggcaat cgcacctatt ctggtttttt tcttgacgat ggcgtcaatt gccaagcata
     1381 aaattgggaa taagacattc gtgaagccta ttttgatctt atatttagtg ggaacattgc
     1441 tttctgcact tgtagcagtt attgtaagtt caatttttac tgttcctatt actttacaag
     1501 aaacagtatc tgaaaaagca ccgcaaagtt tggaaagtgt tttatctact atgttgacga
     1561 atgtcacaca aaatcctgtt caatctctta ttgatgcgaa ttaccttggc gtcttattct
     1621 gggctgtatt gttgggacta gcttttcgag cacgttcaga atcgacaaaa gagttggtgg
     1681 atcagatatc tatagcgttg tcacaagtcg tacaagtgat tattgcattt gctccgatag
     1741 ggattttagg attggtttat caatcaattg ctacgactgg aatagctgga ttggcagagt
     1801 atctgcagct tttacttgtt ttgatcggta cgatgctgtt tgtcgcatta gttgtgtacc
     1861 cattcctcac gtttttgttt attaaagaaa atccctatcc attgattttc ttttgtttga
     1921 aagaaagcgc acttcccgct ttctttaccc gaagttctgc cgcaaatatc ccgataaaca
     1981 tgatgttggc tgaaagattg aaattgacaa aagagtctta ttccatatcg atcccgttag
     2041 gtgctacgat caatatgggc ggtgccgcga ttaccatttc cttgatgacg ttgactgctg
     2101 tacattcttt agggattgaa gcaccttttg ttttgaaagt ccttttatgt attttgtctg
     2161 ctttagctgc ttgtggagct tcaggtgttg cgggcggttc actgttattg attccattag
     2221 catgtagttt gtttggtatc tctaatgatg tcgcgatgca aatcgtcggt attggattta
     2281 ttatcggtgt tatccaggat tcaatggaaa ctgccttaaa ttcgtcaagt gatctgttgt
     2341 ttactgcaat aggagagcta ggcgcgcgta aaagaagtgg ggaaaaaatc aaattgaaag
     2401 attgtttaga tggattaagt gaacgttgaa cgttagatat ttagtatttt taaaatgatt
     2461 cgagttttta atgagttata tagatgttcg tgtaaataga ggtttctgtt aaatcaagat
     2521 aaagtcgctt ataataacaa ttaaaagcat aggtaattgt tattggaggc gtatcatgga
     2581 ttttttacgt aaatttggtt ttttcttttt attattaggc gtgctgagtg attttttgac
     2641 tccgtacgta ttaggattgt attatcctga attagatcaa atgaagagcg tgatcagtgt
     2701 atttggagag gtttccagtc ctgtaagaaa agcgtttctt atttggtctg tggtttcagg
     2761 gatctttttc atcctgtcat tgcctgcatt gtatgctgca tttgtaagta catctcgtgt
     2821 gttagctacc ctagttacag cagccatcgc gttctatggt ataggtgatt gtatctttac
     2881 aggtttgttc agtatcaata cccatgaatc ttcgtggaat gtctcgacat gggtccataa
     2941 tatcggatca ggtttaggtt acagtggttt tttattgttt ccagttcttc ttttcttgat
     3001 ctatcgaaaa aatgggcaaa tggacgtaag ccacttatac cttggactgt ctgtgatcag
     3061 tttgcttttt gcggcgattt atggactggc aagaattcct tctataaatt cttggccatt
     3121 actgaataaa ataggttttt gccaaagaat cagttttttc tttaattatg tgcccattgc
     3181 ctggttgagt atcttgcatc tcaaacagta aaacttgagc cgtcaaattg aacagtcagc
     3241 ggatacttca tccggtggct gttttttgtt tttctgaaat atatatttcc acagttttct
     3301 tgtatagctt ctgttttaga gaagaaagga gctatatttt gtgctacaat cttaaaaagg
     3361 tagagagaag accaaatgga agacagagga gccactaaaa tatgagaaac atcaagttga
     3421 caatcgaata tgacggaaaa agatattcag gatggcaaag acttggagat gacgataaaa
     3481 ccattcaagg aaaaatcgaa aaagtattgc atcagatgac aaatgaagag atagaaatta
     3541 ttggatcagg aagaacagat gctggcaccc atgcgcgagg acaagtagca aattttaaaa
     3601 caaacacaga aattggactg gcagaaatga tcgactttat gaatcgttac cttccgcggg
     3661 atattgtcat caaaagaatc gaagaaatgc ctgagcgctt ccattcaaga tataatgcgg
     3721 tgggcaaaaa atacagctat tatgtttgga acaacgtgat tccttcagca tttgagcgaa
     3781 accacagctt ttatttccct caagagttgg atatggacaa gctgaatgca gcttgcgaaa
     3841 aattagtggg tacacatgat ttcattggtt tttctgcatt gaaaaaatca aaaaaatcca
     3901 cagtgcgtac gattgatgaa ttatctatcg aacgaaaggg agaaatgctt cattttactt
     3961 ttgtcggaga cggattctta tataaaatgg tgcggattat catggggaca ttactagaaa
     4021 tcggcacagg tgccatgccg atagaaacaa tcgatgaaat atttgaaagc aaagtgcggc
     4081 aacatgcagg agaaaccgtc cctgcacaag gtctgttttt ggatgaagtt tattatcgat
     4141 aaaaatactt tgataatgga tgaaacgcat gtttcacagt ataaatatcg atgttaaata
     4201 atgtatgaag caaattaaag
//
LOCUS       DANKKG010000167         4164 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000167 DANKKG010000000
VERSION     DANKKG010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4164
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.168"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..511
                     /locus_tag="PW004_002564"
     CDS             <1..511
                     /locus_tag="PW004_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HDL2727492.1"
                     /translation="EYKNEIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQV
                     EKLSHKSYKDVYDEYSKETDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEI
                     DEHRGELIKNLVTNRAKIQTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKN
                     LVKSYLKMN"
     gene            514..1410
                     /locus_tag="PW004_002565"
     CDS             514..1410
                     /locus_tag="PW004_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2727493.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            1460..1849
                     /locus_tag="PW004_002566"
     CDS             1460..1849
                     /locus_tag="PW004_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL2727494.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            1836..>4164
                     /locus_tag="PW004_002567"
     CDS             1836..>4164
                     /locus_tag="PW004_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL2727495.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         1427 a          645 c          877 g         1215 t
ORIGIN      
        1 agagtataaa aatgagattg aaacatgtgt aaacgaaata agaaaattaa attgggatat
       61 tgtttctgtg aaaaaagaaa tacaagataa ggcgaaggta gagcttgaaa ttgaagattt
      121 aaaaaaacaa gtagaaaagc tatctcataa gagttataaa gatgtttatg atgagtattc
      181 aaaagagaca gatatttata gggaactgaa acgtgatgtg gaggcactta aggaaattcc
      241 caaaaatcta actgatagtg ttgacaaaat tgactttggc aatggtataa aagttacaga
      301 cgaaattgat gaacaccgcg gagaattgat taagaattta gtgaccaatc gggctaagat
      361 tcaaacaata attaatgaaa tgtcagatga aattgatagt tatagagcag agctgaataa
      421 atcagattgg aaagttaact ataatgaggt ttcaaagagg tatcaaagag gtatcaaaaa
      481 cttagtgaag agttatctca agatgaatta aaaatgttac aaaaccttga ttctttatat
      541 caatctttat caaaaactga agagcgattg gaagaaatta ttcaacaaga aataaagctt
      601 aaagattacc aagttgaggt tgactcagca atgtcaagaa tgaataaatc atatcaaaat
      661 ctctattctg agagaagtag ttcctcgcgc gaaatttttt ctgatattac aggaatagag
      721 gctactattc aaccaaatag ggatttcaat aattatgtta aagagctcag agcttgtatt
      781 aataaggagg acacttttga cagtgagttt ggatctctta ttgataagct gacaaaaaat
      841 tccattacta atcaagatat atataatgac atacttgata taagagttag tggggaaagt
      901 agtatttttt cagataaaaa attaatcaat tctataagaa aattgaatga tgcacaagtt
      961 cttgaattac aaacccttaa gccaaatgat ttagtagtta tccgacttga actaaatgga
     1021 aaaaaagtta gtttatcgaa tgctagtgct ggtcaaaaga catctgcaat tctttctatg
     1081 attttggcgt atggaaattc accactcata ttggatcagc ctgaagatga tttggatagt
     1141 caattaatta ataacttaat cgtaagaagt atcattacaa aaaaggaaaa cagacaaatt
     1201 ttaattgtaa ctcataatgc aaatatccca gttaatggtg attcagaatg gttgattagc
     1261 atgggagatt caaaagaaat atcggctgat cagtgcgggt cggtagatga acccaatatc
     1321 aagccaagaa tatgtactgt gatggaaggt ggcgaggatg cttttacaaa tagagcgaaa
     1381 cgctatggtt ttaaacaaat tgtagaataa cactagaggg tgagacatat tattatgtcc
     1441 gcccttttta ggaggaaaaa tgaaaaaaat aaaaagctat accagtatct gggcagttga
     1501 aaaagtcatt tacgcaatca atgattttca gttgcctttt cctgtgactt ttaaccaaat
     1561 ggcttggttc gttttatctt tactgtttgt catcgtattt gcccatgtgc ctccactatc
     1621 tatgatagag gaggtctttt taaaatactt aggcataccg gtggctgtca cttggtttat
     1681 gtcgcaaaag acgtttgatg gcaagaaacc actgggattt ttacgatcct ttatcagcta
     1741 tcacttgcga tttaaggtga cttttgcagg aaagaaagtc aaagaacaga agaaacgatg
     1801 ggatgaaccc agtactcttg taaggagtgt gaactatgta tccgattaag tatattgaaa
     1861 ataatctcgt ctttaaccaa gaaggcgagt gttttgccta ctatgaatta gtgccttaca
     1921 attactcctt tttgtcaccc gaacagaaat ttcaagtgca tgataacttt cgccaactaa
     1981 ttgcgcaaaa tcgtgaaggc aagatccatg cgttacaaat tgccacagaa agtagtattc
     2041 gcgccacaca agaacgatca aaaaaagaaa ttaccggtag actcaaagaa gtagcgaaac
     2101 aacgaattga tatacagaca gatgcattag tatccatgat tggcgatagt cagattgatt
     2161 atcgcttttt tattggcttt aaactaattg cgacagatga agaagtcaat ctaaagagtc
     2221 tcaagaaatc tttcttttct gggcttcaag aatttgtcta tggagtgaat catcatttga
     2281 tgggcgattt tgtttcctta tccaatgaag aaattcgctg ctattctaag cttgaaaaat
     2341 taatggaaag caaattggct cgaagattta aagtcaggcg agtcacaccg agtgacctga
     2401 cgtatttgat tgaacatatt tatggcgaaa aagggatacc gtttgaagaa tacgaatttc
     2461 agctaccaaa gaaaaagcta aaatcagaaa cactggtgaa acgttatgac ttattacgtc
     2521 caaatcgttg tttaattgaa gaaaaaccaa ggtgcttacg gatggaacat gagaatcatg
     2581 agtcgtacgt agcgtattta acaattaata caattgtggg ggagatggaa tttccttcct
     2641 cagaactttt ctattaccag caacaacaat ttacattccc aatcgacaca agtatgaatg
     2701 tagagattgt gacgaataaa aaggctttag ctaccgttcg taataagaaa aaagaactga
     2761 aagatttaga caatcatgct taccaatctg acaatgaaac gaattccaat gtcttagatg
     2821 ctttggattc cgtggatgaa ttggaaacca ccttggatca gtcaaaagaa tctatgtaca
     2881 aactgagtta tgtggttcga gtgagtgccg aatcagtgga tgaactaaag cgacgctgtg
     2941 atgaagtgct cgatttttat gatgatacca atgtaaaact agtccggccc tttggcgata
     3001 tgatggggct acatgaagaa ttcttaccct caagtaaacg atatatgaac gactatattc
     3061 agtatgttac gtctgatttt ttggcggggc ttggttttgg ggctactcag atgctaggag
     3121 aattagaagg gatttacttt ggctacaatg tggatacagg tcgtaatgtt tacctgaaac
     3181 cggcattggc ttcacaagga gtcaagggtt cagtaaccaa tgcgctggcc gccgctttcc
     3241 tgggttctct tggtggaggt aaatccttta gtaacaatct cttagtctac tatgctgttt
     3301 tgttcggtgg tcaagctgtg attgtcgatc caaaagggga gcgaggaggg tggaaagaaa
     3361 ccttgccaga aattgcggat gaaatcagta tcctcaactt gacgagtaag tcagaaaatc
     3421 aaggtctgct tgatccttat gtgattatga agaaaaagaa agactcggaa agtttagcaa
     3481 ttgatattct gactttttta acaggaattt caagtcgaga cggggagaag tttcctgtgt
     3541 tacgtaaagc cattcgaacg gtgactcaaa gtgatcaacg ggggttatta caagttatta
     3601 acgaattgcg aaaagacggg tcaccaatcg cggaaaatat cgccgatcat atcgagtcct
     3661 ttaccgatta tgattttgct catttgcttt tctcagacgg ctcgattacc caatcgatta
     3721 gtttagataa acagctaaat atcattcaag tggcggattt ggttttaccg gatgcagaaa
     3781 cgacttttga agaatatacc accatggaat tgcttagtgt ttcgatgcta atcgtcattt
     3841 ccacttttgc cttggacttt atccatagta accgagaaat cttcaaaatt gtcgatttgg
     3901 atgaggcttg gagttttctt caagtggctc aaggaaaaac gttatcgaat aagcttgttc
     3961 gtgctggtcg ggccatgaat gctggggtct atttcgtcac tcaaaatagc gacgatttat
     4021 tagatgaacg tttaaaaaat aatatcgggt tgaagtttgc ttttcgcagt accgatattc
     4081 atgaaatcaa gaaaaccttg gaattctttg gtttggatca ggaggatcaa agcaatcaaa
     4141 aacgcctgcg agatttggaa aacg
//
LOCUS       DANKKG010000168         4057 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000168 DANKKG010000000
VERSION     DANKKG010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4057
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.169"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            86..1435
                     /locus_tag="PW004_002568"
     CDS             86..1435
                     /locus_tag="PW004_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2727496.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(1563..2153)
                     /locus_tag="PW004_002569"
     CDS             complement(1563..2153)
                     /locus_tag="PW004_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HDL2727497.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            2300..3652
                     /gene="brnQ"
                     /locus_tag="PW004_002570"
     CDS             2300..3652
                     /gene="brnQ"
                     /locus_tag="PW004_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDL2727498.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         1169 a          742 c          788 g         1358 t
ORIGIN      
        1 aaaattttgg aatcttagtt gtgtttttca gaaaactctc ttaatatgga agtaattaaa
       61 cttttttagt taggatgatt taaaaatgaa tgaagagcaa gaactgcagc gtggactgaa
      121 aaatagacat gtccaactga tttctattgg cggagcgatt ggaacggggt tgtttttagg
      181 ttccggaaag tctatccagc tagctggccc ttcgatattg ctcgcctatc ttattactgg
      241 atgcatctgt tttttcatta tgcgtgcgtt aggcgaactt ctgttatcaa acaccaataa
      301 ccattctttt ctggattttg ttgcagaata tcttggcaaa aaagcggctt tcatcactgg
      361 ctggacgtat tggttctgct gggtatcgat tgcaatggcc gatttaacag ccatcggaat
      421 gtatgtacgc tactgggcgc ctggtgttcc tcagtggctt cctgaactga ttgcattagg
      481 tcttcttctt tgcttgaata tggtggctgt ctcattgttt ggtgagttag agttttggtt
      541 tgcattgatc aaagttgtag cgatcattgc ctttatcctc gtaggagctt atatgatcct
      601 cacccactat acgacagata ttggcacagc cagtatcacg aatctatggt ctcatggcgg
      661 attctttcca actggtgcaa aaggatttat cttagctttc caaatggtaa cctttgcttt
      721 tgctgggata gaattagttg gattagtcgc tggagaaaca gaaaatcctg aaaaagtgtt
      781 gccggaagct atcaataata ttcctatcag gatcatctta ttttacttag gatctctttt
      841 tgtcattatg gcaatctatc catggaacag cttgaatcca gataacagtc catttgtaga
      901 agtattttcg gaaattggga tcacgattgc tgcttccttg atcaatttag tcgtattaag
      961 tgcagcagct tctgcctgca acagtgcaat atatagtaca ggacgtatgc ttcgttcttt
     1021 agcgcaagaa ggcagtgccc ctaaaaaatt caaaaagtta acaacacatc atgtgccagg
     1081 gaatgctttg acattttcta ctatcgtcat tttcatttct gttatcttga attacgtgat
     1141 gcctagtgaa gtattcacac tagtaagcag tattgccact acttgtttct tgtttatctg
     1201 gagtatgctt gtctacacgc acatgaaata tcgtaaaagt atcctcggca aaaaagctca
     1261 ttcatttaaa atgccgttgt acccatttag taattatctg gtatttcttt acatggcctt
     1321 tgtgtgtgtc gtcttgttcc taggaaaaga tacgagaatc gctttattac taacacctgt
     1381 gtggttcttg cttctattac taatctatca tatgaaatat gaacgtaaga agtaagaaag
     1441 tagttgtgac agaagtggtc agcttcgaga aataagaagg aatttacgaa aattgcttct
     1501 taaatttttg tgaaatttcg gcttatttct tggagataaa agcttctgtc ccagcctctt
     1561 ttctatttcc ccggaaacaa ccgctgataa taggcatctg cttttttata ggaaaaaatc
     1621 caaataccag atacaaaaaa acgtccgaat aacccgatca acggtacaaa aaagacaccg
     1681 gcaatcaagc ataagttaat catcatggag ataagacttc gacggtcctt tgacaaaact
     1741 ctcacgacat gggaatctgg tttatttgct ttcatgattt gcctcatcaa gataccaaaa
     1801 gaaaaactcc acatcgtgta tactaagaca taagccatct caggccaaaa agcttcaggg
     1861 ttacgcccga cccaactagt aacagaagga cataaagtca gccaaaagag caaaaagata
     1921 ttcgaccaga ccactcggta attaattcgt tctgccatct gaaatatacg atgatggttc
     1981 acccagatca ccgcaacaaa tataaagctg atggtataag ctaataatgt atttcggatg
     2041 acccataaag aatgaaagct agatgtatta gggatcttga tctctaatac aagaactctc
     2101 aaaacaatgg ctaccactcc atcagtaaat gcttctaatc tcgactttgt cacgatgatt
     2161 cctcctcaga tgattcagcg cttttatttt atcacttacg ttattaaata accatcgttt
     2221 tatataggga attttctgaa aattgttgca tatttttcga aattttgtta cttttaaaaa
     2281 aaccgtaaag gagttatgta tgacaaaaaa attatcattt aaagactatc tttttatagg
     2341 ctccatgctt ttcggtctat tttttggtgc tggaaatctt atctttcccg ttcatttggg
     2401 tcaagaagca ggcgctgcta ccttttgggc gaatctaggt ttcttggtta ccggtatcgg
     2461 tcttcccttc ttaggggtga ttgccattgg tgtatcgaaa agctcaggtg tatatgaatt
     2521 agcaaaacgg ataaatcaaa catatgcgat ggtttttacc atcttgcttt acttagttat
     2581 cggaccgttt tttgctttac ctcgccttgc aaccacatct ttcgagattg gtttagcacc
     2641 atttatcgat cctagcagac agacgctata tctagcaatt ttcagtattg tattttttgt
     2701 gctggcttgg tggttttcaa gaaaaccgac aaagatatta gattatgttg gaaagttcct
     2761 gaatcctgca tttttggttt tattaggtgc tttgcttctc cttgcttttt tgaatccact
     2821 aggttctgta aatcaagcgc ctattcagcc aaattatcaa gagcatgctt ttttcacagg
     2881 gtttactcaa ggatataaca cattagatgc gcttgctgct ttagcatttg ggatcatcat
     2941 tgtcacaaca atccaaaaca tgggtgtaac aaaacctgct gaaatcgcta aagacacgat
     3001 caaatctggt gcgatcagca ttatattgat gggaatcatt tatactttgc tcgcttattt
     3061 aggtgctatg agcttaggta gctttgctct aagtgaaaat ggcgggatca cgctggctca
     3121 gattgctgat cattatttag gtacttatgg cagtatctta ttagcattta tcgttatatt
     3181 agcttgcttg aagaccggta ttggtttgat tacagctttt gcagaaacat tcacagattt
     3241 gtttccgcaa agaaattacg cattctttgt tgcacttgct agtatccttc cttgtttagc
     3301 ggcaaacgta ggcttgacga atatcattca attttcgttg cctgttttga tgttcatcta
     3361 tccattagca atgacactga ttttactagt gttagttggt cctttgttta agcagcgtac
     3421 ggctgtttat cgaatgacta cttattttac attgattgct tcgatttttg atgggttgaa
     3481 cgcttgccca gaaactatca aacaaacacc tattgttcaa aatcttttat atgttgcgga
     3541 aagctacttg ccgtttttca aattggggat ggggtggatc gtaccagcag ttatcggttt
     3601 tgttatcgga ctgatttgga gtttcgctaa gaaagaagaa gtagcagatt gataaaagag
     3661 gttgtgaaag agacgttcag ccttgagcaa taaaaagtaa ttttgaaaaa tggcttctca
     3721 catttttgca taaatttgac ttaataccga gaaactgact tttgaacacc gtttattgag
     3781 aggtcgtgaa aaagccgctt agtttcaagt attaagaaat attttgtgaa aatggcttct
     3841 cacatttttg cataaatttg acttaatacc gagaaactgg cttttgaaca ccgtttattg
     3901 agcgtctata aactgacctg agtattagag aaacaaaacg gcaaagtagc tccatatact
     3961 ttgccgtttt gttttgaact aataccgaag agaagctgat caaacaacat ttcttttata
     4021 atatagattt tagacatata tatgatataa aaatagt
//
LOCUS       DANKKG010000169         4046 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000169 DANKKG010000000
VERSION     DANKKG010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4046
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.170"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(204..1346)
                     /locus_tag="PW004_002571"
     CDS             complement(204..1346)
                     /locus_tag="PW004_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2727499.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(1351..1536)
                     /locus_tag="PW004_002572"
     CDS             complement(1351..1536)
                     /locus_tag="PW004_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HDL2727500.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(1890..2135)
                     /locus_tag="PW004_002573"
     CDS             complement(1890..2135)
                     /locus_tag="PW004_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2727501.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(2129..2530)
                     /locus_tag="PW004_002574"
     CDS             complement(2129..2530)
                     /locus_tag="PW004_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL2727502.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(2717..>4046)
                     /gene="uidA"
                     /locus_tag="PW004_002575"
     CDS             complement(2717..>4046)
                     /gene="uidA"
                     /locus_tag="PW004_002575"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HDL2727503.1"
                     /translation="ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASV
                     EFNIDTEWTGKILIAIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLID
                     DNKVIDTYTEEFGIRSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDF
                     NLMKWIGANSFRTSHYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKH
                     NTWKEIRTENSHKTVIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKE
                     LDPQKRPVTIVTYLKSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDG
                     WMERCPDTPIMFTEYGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQV
                     WNFADFATSQGILRVQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
BASE COUNT         1276 a          770 c          527 g         1473 t
ORIGIN      
        1 aaaaaccacc ggtggcaaac cggtggcaaa tccatgtaaa catggtttat tttttacact
       61 gttttcaaac aaacccttgc tatttcttcg gttcgtcctc gtccattttc gataccgaca
      121 gctagcttat aacccaaggt atatcaacat aaaaaaataa aaataggcta tgacagcagc
      181 gtgacagcaa aaaagatgga aagttaaata tcaaacctcg taatttcatt tgcgagtgct
      241 tgatttgttt ctggatataa gtgtccatat ttgtccaaag tagttttaat agaggcatga
      301 cctagacgct tagatattgc atagatattt ttattatgtt caattaaaaa ggcaacatgc
      361 gagtgtctta atccatgcat atgaattggt tttactcctg caatttcagc tagttttttt
      421 attctgctta aaatagttct tggagatggc ggtaatccat caaaagaaaa aaccaattct
      481 atatttttcc caattgattg ttgagttttt ttccatgttt ttaaatcttc aacggtttgt
      541 ctgtctaacg aaattatccg aatacttgat gtattttttg tttcagcaaa ttcataatct
      601 tgtcttgtac ggatgtaaat tgacttattt acgctacatc tgccatttat taagtcaaag
      661 tctttccatt gtaacgctaa gagctcctca ctccttacac ctgtaacaaa aataaaacgc
      721 atcaaacgtt tataaaattc ttcataaaaa tccccgtgat aaacactatt atatactttc
      781 ttaaattcat caactgtcca aaattctact tgaggacgct gagtttttat tttacctata
      841 gtatctacag gattttcttc aataatttca agtacaattg cccttttaaa aactactctc
      901 aacttattga atatctgttt aatataattt ggtgataatg gttgctcaat gccctttttt
      961 acaacatgga ttttcgataa aaactgttga aattcctgta tatgaattgg tcgaatcttt
     1021 acaacctgca tttctccaaa aaaatccaat gcacgttgta aaatatgatc tgttttcata
     1081 tatgtttttt ctacagttcc caacttatac caaggaaaaa aatactctcg ataaaaatca
     1141 tgaaacgtta ttccttgcag ataaattcct tgtgaatcag aaaaactttg ttttaactct
     1201 tcgagtcccc ttctagcttc tccttgagtt ttgaagcctc ttctggtcac ttgaattctt
     1261 tttccaaagc gatcttgtcc taaagttgca gaaaaaaacc aagtgcctct ttttctgtct
     1321 ttataaacat tcgcaatttt cctcatttta ctattcctcc caagaaatat ggagaacttc
     1381 ttctaaaatt gaaataggaa ttctttttat ttttgaatta cgataaaaaa agtatccttt
     1441 ctcaactaaa agttctttac attccttgaa gattttatta gcatagtact gagaaaaacc
     1501 tagattaatc aaatcttttc gagtagctgt tttcattata gcaccttcct tacttttttt
     1561 catttatttc tatcagctgc ttttgtcaaa agcacatagg aatttcacct ctccccaagt
     1621 ttacggcgag ccttacggaa gtatcattat cccaacttta aatcatcgct ttttattgac
     1681 tataaaaatt attttagaat atttatcgct ccttctttac agcaagaaat catggcgcaa
     1741 ctaaaatatg ctccttaaag tgttaacgta ctgatagaat ctatttaatt ttcaatgaac
     1801 aataggtttt ctacctaaaa gaatctacta attccttaac tattagaaga ttcttttaaa
     1861 cagaaaaagt ggaaagggga tttttttaat tagttaattt taaattttaa gatcgcttct
     1921 attaatttaa tttctagctg acttatcata tattcatcta cataggtaac gaattgacct
     1981 acctcgttaa atccatttgt caaacataac ttagccatat aagaatgata atgtttgagt
     2041 atcatcttga tcgaaattgg atctccttct agagcagata agataatgga tagtggaact
     2101 aataggtact gccgcttttt agatttatct accataggtc ataccccatt ttcaattgtt
     2161 caactgcctt ctttcgttga taccagatac cacttgtgct catattgaga attttaccga
     2221 tttcagcatc attaaaacct gcaaaataat aaagtaggat aatctttctt cttatctccg
     2281 ataactgatc aattgctgct gataactttt gatctgaaat taatatttcc attccaaata
     2341 agaaatagac atcttgttca tcaaaatgag tttgggaata cctgccttca tttgaatctg
     2401 ccaatggttc ctcattatcc tttatgttag cgtttcgttt tcgaatagac ttagcttcgt
     2461 ttcgtaatac ttttttacag taggcatcaa attggtgttg tatagatatg tcattgtaat
     2521 gttgttgcat gataatatgc tcctttttcc ccctttccac taatacaaca atcagaaagc
     2581 cccttttttt cgaaaaaact agaaaaaaaa cataaaaata tttatcattc tttaaacaga
     2641 aaatctttct ataaaaaaca aaaaaaaacg attaggctag gctaatcgtt ttttgaacat
     2701 tcttaaaaaa tccaaattat tttttataac tgaaattggg tattgatgac catcgtttct
     2761 gtagatagta agcaatcatt ttaggttttc tatctctagt aaagattcct ttcttatttc
     2821 cttgcactct taaaatccct tgacttgttg caaaatctgc aaaattccac acttgttcac
     2881 caacaaaatt ttcacatgta tctattactt cgtgatttgc ttcgtaataa gccacttgat
     2941 actcttctgt aaacattgtt ggtactgtat catgaaatcc agctactgta tctgctccat
     3001 attctgtaaa cattattggc gtatcaggac atctttccat ccatccatct agctcttttt
     3061 tcaacagata ttttgcatgt gccagatccc ctcccgaaac ataccaacca taataacgat
     3121 ttaaacataa aacatcgaca atatctccta ccttacaaac atctggcgtt gattttagat
     3181 aagtaacgat tgttacaggt cttttctgcg gatcaagttc tttagttaat tcaactaaag
     3241 gttcaaaata ttctttagca ccttcggaat ccgaatctgg ttcatttgca atagaccaca
     3301 ttacaacact tgggtggttc ttgtcgcgtt ctatcaattc cttgatcacc gttttatgag
     3361 aattttctgt tcgtatctct ttccacgtat tatgtttttc cacattatca tctaataaag
     3421 tggccatgaa atttaaatgt aaccctactg caggtacttc atcaataact acaatacctt
     3481 ctcgatctgc taatcgcata atttcttctg agtaaggata atgtgatgtt ctaaaagaat
     3541 tagcacctat ccatttcatt aaattaaaat ctttaatatt tacagcctca ttcaaaccac
     3601 gtccattaac atacgaatcc tcatgcttac caaatccttt aaaataaaaa ggtttattat
     3661 taatcaaaaa tttgccattg gtaacctgaa cactgcgaat accaaattct tctgtatagg
     3721 tatcgatcac tttattatca tcaattaaac taattttcat actatataaa taagcgtcta
     3781 gaggttccca aaaaattgga ttcttaagct gaatcattcc tttctttcct tcagcttcag
     3841 ctacaagttg agcctcttcg tcatatatcg ctattaagat ttttccggtc cattctgtat
     3901 cgatattgaa ttctacactt gcatccaaat cagttattct tgtattaata gtaatatcac
     3961 ttatatagtc tttcggagtt gtatataact taacaggtct atgaatacct gcataattga
     4021 agaaatcaaa attaactaag ttttcc
//
LOCUS       DANKKG010000170         4027 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000170 DANKKG010000000
VERSION     DANKKG010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4027
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.171"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            293..1261
                     /locus_tag="PW004_002576"
     CDS             293..1261
                     /locus_tag="PW004_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL2727504.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADARNHDLDVRRYMGKVEY"
     gene            1272..2087
                     /locus_tag="PW004_002577"
     CDS             1272..2087
                     /locus_tag="PW004_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HDL2727505.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKKIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2155..2865
                     /locus_tag="PW004_002578"
     CDS             2155..2865
                     /locus_tag="PW004_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2727506.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYVMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            2901..>4027
                     /locus_tag="PW004_002579"
     CDS             2901..>4027
                     /locus_tag="PW004_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDL2727507.1"
                     /translation="MKQVLRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIK"
BASE COUNT         1346 a          596 c          872 g         1213 t
ORIGIN      
        1 aaacgatttc ttctaaacaa atcaagacaa acttataaac cttcatccca gcaccataat
       61 tatttaaatc ataggcattt ttcgaaatta gtatatgtac tcaagtttca ctgcagatta
      121 tttatatttt ttagagaaat aaatttttcc tattgacaaa cacaaagaaa gcggatacaa
      181 tacaattatg tcacatgacg ttatatgacg ttatgtaata tctttacatt taatcagtta
      241 cttttatttt ggataaaatt aacattttta aaattttaag gaggaaatgt tgatggatgt
      301 agcaaaaacg gttaacgcaa ttgtagaaaa aaatgatgga gagatttctg gaatttattt
      361 ggtggcttgt ggcggttcgc tggtagatat gtatgtctca gattattttc ttaaagccga
      421 agcatcgaag tgtattacag gcttatatac agcgaacgaa tttaaccatg taaccccaaa
      481 gcaattagat gataaatctg ttgtcattct atgttctcat ggaggaaata cagcagaaac
      541 ggtaaaagca ggagaagtcg ctcaagctag aggagctgtg actgttggtc tgactcataa
      601 taaaactgcg gatttattaa aggtttctga tcatagtttc ctttacgaat ggggagatga
      661 tgcaaaaata gtcaataatc ctatggccat cattcttgat ttaactgtat ctttggtcaa
      721 tgttaccgaa acatttgaac attacggtaa attcagagaa ggcatgaagg ttatcgatac
      781 cgtaattgaa aatggcatta agcaggtgca ggatcgtatt aaagtgtttg ctgataagta
      841 caaagatgaa acaatgtttt atatcttagg tagtggacca tcatatggac atgcttatgg
      901 tttttcaatt tgctctttga tggaaatgca atggcttgat tcaacttctg ttcattctgg
      961 agaattcttc catggacctt ttgaagtaac agacaaagat acgaatttta ttctcttgat
     1021 gagcattgga cgcactcgtg ctttagatga acgggcaaaa cgattccttg ataaatatgc
     1081 agaaagagta gaaatcgttg atgcaaagga actaggattg gatatgatat cagatgaagt
     1141 ttctgaattc ttcaatccca ttttattcta cagtattctt tcagaatatc gttcagcttt
     1201 agcagatgca cgcaatcatg atttagatgt tcgtcgctat atggggaaag tggagtacta
     1261 ggaggattaa aatgaaggta ttaggcttag gtgataacgt cgtcgataaa tatgaaaatc
     1321 tgggaattat gtatcctgga ggaaattcat tgaattttgc agtttttgca aaaaaactgg
     1381 gacatgaaag tagctttatg ggagtttttg gctcagatga ggaggcagct catgttttat
     1441 cagcaataaa aaaaatagga ctggacaaca gtcactctcg ctttgaaaaa ggcgagaatg
     1501 gctgtgccag agtgaagatt gatgagggcg accggatttt ccttggcagc aattcgggag
     1561 gtgttactcg tgaatacccg attcagctta ctgaagaaga cagagaatat ttaaaccagt
     1621 ttgaattgat tcatatggga ttatatagcc atgtcaatca tctattagaa gagttacaaa
     1681 atgtttctgg aaagatttct tatgattttt ctgacgactt tacggaagaa gaggttcagc
     1741 gggcaattga taaagttgat tttggcttct tttctataga agacttttcc gatgaagaag
     1801 taaagcagtt tttgatgaag catttttgtg agcgaaatga tgtattgatt gcaacccgag
     1861 gagaaaaaac ggctattggt tatgatggag caaagtttta tgaagtgaac cccgtgctag
     1921 aagaaccagt tgatacaatg gcagcgggag attctttctt aacagctttc ctactttatt
     1981 ttctcgaagg ggacgatata gtcaccgcga tgaaaaaggg gaatgaattt gctgggaagt
     2041 cttgtctggt tgacggttcg ttcggctttg gtattcatta cgattgagct tgttttaaat
     2101 tcctgcattt gataacctaa agaaagaaat cccggtaaag tggaggttaa aaaaatgctg
     2161 gatacaaatg cacaattacc attgtactta caattaaaag aaatattaaa aaataaaatc
     2221 aatacagggg agtatcccga gaatacgaaa attcccgctg aatcagagct gatagaaagt
     2281 tatgaggtca gccgaatcac agtgagaaaa gcagtagaag aactggtaga agaagggtac
     2341 ctacttaaaa aacaagggaa aggaactttt gtcaaccggc ataaagtttt tcgtaaaatt
     2401 gaatatgtta tgggtttcag tgagagttgt aaatctaatg gttttacacc gactagcatt
     2461 gttttggaac gcaagatcat tccagcaact gaagggatgg caaaaaaact cgatatcgct
     2521 caaggtgatg atgttttata tatccagaga aagcgaatgg cggataacat ccccattatg
     2581 ttagaaaaca attatttttc ttatgaaaga tatagtttct taatggagga agagctgact
     2641 gattctttgt atgaagtact taaagtccaa ggtaccttag ccatcaatcc tggggaaact
     2701 gcacttgaaa ttgtcacagc tgatgaccaa attgccaaaa taatggaagt agctgttggg
     2761 acgccctttt tctttatgga tactttaatc aacgatcaaa acaacatacc aattcatgta
     2821 ggtaatcaat attacctggg agaagtatat cgtttttact tatgatttcc tgttttgatt
     2881 tcaaggagta gattttattt atgaagcaag tattgcggtc aaaaatgatt tttgatggag
     2941 agcagccacc tttcaaaggt tatattgtaa tcaatgaagg gatcattgaa aaggtggaaa
     3001 acaactggaa ttacagtgat ctattaaatg aaaacacgga attaattgct tatgatgatc
     3061 aattcgttat gccagcaatt catgataatc atgtattttt cagtggttat ttggctatga
     3121 atgctggcat agatttaagc gatacatctt cagctaacga ggctgtagag cgtattaaag
     3181 ctgtaatgaa ccataaaaaa gcagatgaac caatttatgc tcatggctgg gatgctgccc
     3241 tgtggaaaac tgacccatca gaagagctat tgaatgaaat aaaccacttt ggtccaatca
     3301 cagcgatcga caaaaatcgc agccattgtt ggatgaatgc tattgctaag cagacttatg
     3361 gttttacaga aaaagaaacc agtgctgaaa gtcgagtatt attaattaag gaaatgctag
     3421 caaacaaaaa gcttttagca gaggtttacg ctgaatttga agcattactg ctttcaaaag
     3481 gtgttatatc aatgaaagaa atcgtttttg atgacgcaga atttctttct aatatacctc
     3541 agaggaaaat acttagtaac ctatatgtgc aggcagtcca agaacctcta cagcatgaac
     3601 ttctatatga gtataaggaa aaagttttcc ctgaaaaggt ccgctttggt ggagtgaaga
     3661 tcatggtaga tggtgttgtt gcggatagaa gtggagatat ttacggtgtt tatcagtcag
     3721 gagtagcttc acccgagatt gactatgagg caattaaagc agaagttgct gcattcaatg
     3781 aagtagagat tccttgctgt ttgacaacgg aaggagataa agcgggttat aaagctgcgt
     3841 caattcttgt cgaatttgga aagcggttac ctgaaggcgt ttttaattcg attagtgatt
     3901 tggaaatggt tactcaaaag acagcagaaa aaatgaacga aggacaagta gtagcagagg
     3961 tatatccaca aattctcggc ttgaatcaaa cgttagaaga ggcttacatg tcaactgtta
     4021 taaaaaa
//
LOCUS       DANKKG010000171         4025 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000171 DANKKG010000000
VERSION     DANKKG010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4025
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.172"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(232..381)
                     /locus_tag="PW004_002580"
     CDS             complement(232..381)
                     /locus_tag="PW004_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727508.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            843..1055
                     /locus_tag="PW004_002581"
     CDS             843..1055
                     /locus_tag="PW004_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727509.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1333..3132)
                     /locus_tag="PW004_002582"
     CDS             complement(1333..3132)
                     /locus_tag="PW004_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDL2727510.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3256..3852)
                     /locus_tag="PW004_002583"
     CDS             complement(3256..3852)
                     /locus_tag="PW004_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDL2727511.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1443 a          711 c          689 g         1182 t
ORIGIN      
        1 ttatacagat aataacggaa tattatcatc gaaacagcta acaaaattgt cataaaaaac
       61 gagatggttg atattagaac ttgtgttttt gtttaggtct agagataaag agagacaatt
      121 tccaaatata tgtaatgaat acaaaaagac gtttgtcgcg gattaccgtt acaaacgtct
      181 tttattgatt ttccgagatc tcatcagtcg ccatttggaa tggttcactc cttatctaga
      241 ttcgtttaat gctcggactg cttgttcgat tgtaatacca aacgcaacgt tattttcgtt
      301 ttgtgcatca atcgccataa aacggttaga agttgtatca aaagctacgc ggtattgcat
      361 atttgttttt gtaaacgtca taaaattcat ctcctttgta atgtaattta gttcttgaaa
      421 gtttccttta aactttcgaa cttataccaa gtttagcatc gtacgttttt tgatgcaatt
      481 aaaaagcatt tatcattttc cttatttatt ttttcttaaa tggtttagct attttctttt
      541 aatccgataa gtagcccaat catcaaggtt acaaatacta ttgcttgaat ataaatcata
      601 tgctcacctc tttataaata acttagcaat ataaagaaaa aaacgcacga acaacatagg
      661 aatataaaca aacgtttttg tcacaacgtc taagatcgtg ttaaaaaata gttatctaaa
      721 aagcagaaag atactcaaaa taaaaaataa aaaaatggat aaaaacgttt tcaaatacaa
      781 ggtaatttgt tattataaaa taaagaaaaa acaaaataaa aataaagaaa agagggaatg
      841 aaatggtaaa agcagaagta ggaagatctt atatatgcaa accgatcgga gtatctcatg
      901 aactaatcgg ctgtattgaa cgtacatata tcaacacggc tgtcgtgtat gttgaaagtt
      961 atgaccaacg agactatcaa cttatcaatg aaagcaagta tcgcatgctc gtaaaattct
     1021 gtgatattag cgcacctgct gaattggttt cttaaacagt atttttgaga attcgcaaga
     1081 actcccttat aatataaata aagacagaag ggagcgagaa gatatgccaa aaagaaaaag
     1141 ttcagggaaa atgggaatta ttcttgcagt gatctctgca attcctgcta ttttagctat
     1201 tgttcgtgcg atgaaggaat caagagaaag ttaagcacac attatgagcg tcaaacaaaa
     1261 aacgcatata cctgcggtgg caggttatgc gttttttgtt gtataaagac ttttttcatt
     1321 aacgattttt gattagccga ctcccaaaac aaaatttagc agtttatctg aataagtgat
     1381 ttcttcatct gtttgtttca cactttcatt tagtagtccc atctcatcgg ttatgatacc
     1441 atccacacca taaaacatca tgcgactcat agcatctgaa tcgttgattg tccatacata
     1501 tacttctttt ccgtcttggt gggcagaatt aatgaagtta cggttgatcg tactatattc
     1561 cattgtcaga aaatcagcag gcgtcacagg gggcccaaca atattgaaag gtaagatgta
     1621 gcccacataa aagtttggtt ctgcttcttt taaatctgaa acggtttggt acgtcaaact
     1681 ttgcaaaata tgatggttct ctaagataac tttgcggtat ttatggacaa atcgttcgac
     1741 aaggtcatcc gaatcttttt tagaggtctt gatctcaata agtaattttt ggtttagctt
     1801 atgtgctttt tccaaataag aatcaaaaga acaaacaggg gcttgcactc cgttttcagt
     1861 aactgtaaga tttgtcaatt ctttcaatgt aagatcctgt ggtgttccat ttacccctgt
     1921 taagtgacga agatttgcat catgcatcac tacgaaatga ccatcttttg tttcctgaac
     1981 atccatctct acataatcag gatgatagtc tctgcttgtt ttttcaagtg cttctaaagt
     2041 attttgcgcc ccattttggg tactcacacc tctgtgagag acagtcactg gttttgtctg
     2101 aacagctgaa gtcagataat ccatattgta aagacagacg ccaataccga aaaaaactgc
     2161 aaaaagagtc aaaccagtgt ttttcagcgc agagaaacgc aagttggggg cttgtgggat
     2221 aaaccactta gggatctcag gcaagaagcc ttcgtcatcc atgaaatcaa caatgatata
     2281 aaaaatccct acggtagaca tcacgatatt gaataataaa atcccttgta aaagcgtcat
     2341 agcaaacaca gcgctgatca atgcatagtc tgggaaaaat tgttcaacca tactttgtgc
     2401 caaaataaca acgatatatc ctaaagaaga aaggagtaaa atcgtaccgc caatcacaat
     2461 aaattggccc gtgatggcca aaagacgcga tttggtcaat gaccagcttt cccgaatggc
     2521 tgctcgaaaa ggacgatctc gtagaatcat ttccggtaaa gcaaaaatca gtcgaatccc
     2581 aatatagcca aggaaaatat aaacgagtaa aaaggaactg acgatgatcc aacgattagc
     2641 aaaaataaaa tccataatga aagcggggat ttttattttt gacagtagat cagaattaaa
     2701 acttaatccg ctgatcggta gaatcaataa aaaataagca aggaaaaaca aaaaagtaat
     2761 gggtctcact ttcttcagct gaagtatcgt caagtgtaag agttgcttca aagagattgg
     2821 ttctttcttt ttaataaaaa aaacactcat caaaagaaaa gtaaattcga aataaactaa
     2881 cagtaaaatc gtcatcaaaa ctacgattaa cgagagtaga accccaggat gttgagaaaa
     2941 aattgaagga atattgtcat aagacaaata gtcaattgcc cctcggtgta aaataaaacg
     3001 ggtcgtactg ctcaataagg gtaaacaaat aaataaaata aagccatgca tcagcaagac
     3061 atcacggaaa taagcttgtg ttccttttaa aaagtcccaa gcattaccta agctacgttt
     3121 caaataattc atcctatcac ctgctcttca cattgtagcg caaatctaat gaaaaaggaa
     3181 agggtgcagt gtgttttgca gaccctttcc cagatttaac attgtcaatc aatgactaat
     3241 tttacaaaat aaatattatc caagaagacc acttagatat tgacctccat aaatcaaggc
     3301 catactatat aagaaaatcg acagtggttt agcaatcaaa atgattagtg tatatcgttt
     3361 cactgacatg tttgtcaatc cagccatcaa acataacgca tcgtcaggag caactggaag
     3421 aaaaatagct agagcaaaca gcttctcaaa acggttttga ttatccagcc agccaatata
     3481 tttgttatat gtcttatcgc taatcatgga aagtatgaat ggctttccat aacgacgccc
     3541 tagcagaaaa atgataatcg aaccaatgca gattccaata tagttataga taaatccagc
     3601 ataagggcca aatatcagga caccagctgc agtactgatc cctccaggaa tgattggaat
     3661 aactacttgc aggatttgga tgaagacaaa aataatcggt cctaaaatga tggaatcacc
     3721 tactaatcct cttaacgcat tcaaatcttt gaaaacacct aaattaataa aataaatcgt
     3781 cagcgcgatt gataggccta atccgataat ggatatgaaa tttatcagtt ttcgagaggc
     3841 agcaatattc attattcatc aactccttag aggttctttg ttaactatac tttactagaa
     3901 aagcaactaa ctgcgccatt ttttgatatg acagaaaaca aacaattttg taagttcatc
     3961 ataagtataa aagattgatc tcaaaaaaac atccaactaa agacttattt aataacttgt
     4021 ttttt
//
LOCUS       DANKKG010000172         3964 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000172 DANKKG010000000
VERSION     DANKKG010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3964
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.173"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(73..1578)
                     /locus_tag="PW004_002584"
     CDS             complement(73..1578)
                     /locus_tag="PW004_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL2727512.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1575..2261)
                     /locus_tag="PW004_002585"
     CDS             complement(1575..2261)
                     /locus_tag="PW004_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2727513.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2454..3875)
                     /gene="gndA"
                     /locus_tag="PW004_002586"
     CDS             complement(2454..3875)
                     /gene="gndA"
                     /locus_tag="PW004_002586"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HDL2727514.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1091 a          844 c          682 g         1347 t
ORIGIN      
        1 aatttctcac ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt
       61 aaagcacatg ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt
      121 cccttgccct actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt
      181 agcgatagac aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg
      241 ataaaaacga ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg
      301 aatcgcgatc tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt
      361 agttgaatac tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg
      421 ataaatttgc agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg
      481 atataatatc ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc
      541 attcgcataa tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa
      601 gcttttcata cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg
      661 atcatctttc ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt
      721 ccttaactca tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat
      781 acggtcaagc atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat
      841 ttcaggcatg tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag
      901 taccttgagt ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat
      961 caaagaaaca acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa
     1021 agaagataat tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata
     1081 tataggctgg atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt
     1141 acgtgtagtc tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc
     1201 caaatcataa acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat
     1261 cttcattaag tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt
     1321 cgctttcgtc agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa
     1381 acgagaagct acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag
     1441 attgatagat gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa
     1501 agaactcgca aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga
     1561 ttttttcggc tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag
     1621 ctttcctccc cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg
     1681 tttgtttcaa cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat
     1741 accacgttca cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca
     1801 attacttcat ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa
     1861 gtaatagtag tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat
     1921 gcacgaagac gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct
     1981 ccgtggtcaa gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt
     2041 ggtgtatttt tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc
     2101 atcagatcca acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg
     2161 ccgttgtagt ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt
     2221 gccaagttct tttcatcttc aatgattaga atattgctca tttattattt tatcctttct
     2281 attctactgg ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag
     2341 tcattttatc atggatagca attttaaaaa agagctgaag tctactcata acaatcgact
     2401 tcagctcttt cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct
     2461 tcatggtacc atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg
     2521 ccaaagtaat cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa
     2581 tcgaagtaag cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag
     2641 gcaacgacgt ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct
     2701 agcaataaat tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat
     2761 cgagctcgga tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc
     2821 caatcaaatt cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt
     2881 ttactgaaat acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac
     2941 tcaaaatcgt tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca
     3001 gaaatatagc gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc
     3061 gcgctttggc ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct
     3121 actactggtt tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct
     3181 atcaaatagc tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct
     3241 actgaaaggt ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct
     3301 ccatactcaa taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata
     3361 tatgtgacac atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca
     3421 actagcgcat aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct
     3481 tcttcaccac cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca
     3541 ttacgacgca tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta
     3601 tcaaggtgag gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt
     3661 agcaagatcc gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct
     3721 ttgaaatttt tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg
     3781 aataaagcca ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg
     3841 gccataccga caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt
     3901 tctccctaat ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat
     3961 attt
//
LOCUS       DANKKG010000173         3943 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000173 DANKKG010000000
VERSION     DANKKG010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.175"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(246..923)
                     /gene="nth"
                     /locus_tag="PW004_002587"
     CDS             complement(246..923)
                     /gene="nth"
                     /locus_tag="PW004_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HDL2727515.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(930..1628)
                     /locus_tag="PW004_002588"
     CDS             complement(930..1628)
                     /locus_tag="PW004_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HDL2727516.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            1896..3080
                     /locus_tag="PW004_002589"
     CDS             1896..3080
                     /locus_tag="PW004_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HDL2727517.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3086..3529)
                     /locus_tag="PW004_002590"
     CDS             complement(3086..3529)
                     /locus_tag="PW004_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL2727518.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            3768..>3943
                     /locus_tag="PW004_002591"
     CDS             3768..>3943
                     /locus_tag="PW004_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDL2727519.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKV"
BASE COUNT         1045 a          774 c          670 g         1454 t
ORIGIN      
        1 ttatataatc ctttggaata ctggacttat tttataggta tattggaagg cattattttc
       61 tacatcgggt attttcccaa taaaaataac aacttttatt tttatcgcaa taaattgtaa
      121 taagttacca tcctagctac tgagtatgta catttacttt ttcatgcaaa aaagataaac
      181 gaaacagctt ttctccataa taacaagata attcatacat ttatttgcaa ttaaagaaac
      241 cacgtctaca ggcgtggttt cttttttagc gccttttctt ttagacgcat ccgatttttc
      301 ccatcttgac atattgatag caaaggacat acctcgcact tgggatttct tgccgtgcaa
      361 tgatatctac caaaaaaaat cagtgtatga tgtgtcttta cccataattc ttgtggtact
      421 ttccgcatca gtgtttcttc tacttccatg accgtagcat ctagtttgca gatcctcagg
      481 cgttttgaga cacgttccac gtgggtatct actgcgattg ctggtatccc gaatgcatcc
      541 cccaatacga catttgctgt ctttctgcct acaccaggaa gagacatcaa ttcttctctt
      601 gacgttggca cttgcccatc gaaacgttca atcagctgct gtgcacacgc tttgatgttt
      661 tttgctttat ttcgatacaa accaatcgtt tttattttaa gaatgatttc atcgattgaa
      721 gcttctgcta acgcatcggg tgtcggaaaa gaggcaaaca aatctggcgt tgctttattc
      781 actgatacat ctgtcgcctg ggcacttaag atcactgcaa tcaataactc aaatggatta
      841 ttgtgcttga gttctccatg tgcttcggga aacattccat acatcgtttc tagagcttcc
      901 attgtttttt gtttgcttaa catattccgc tactcctcca accaattttt cagtgagact
      961 ttcggcaatt cttcttgttg agctgactgt tctacttctc tttgaagaag ctgctgtttt
     1021 cttcgttttt ggtcttcttc cacttgttgt tttgtacgta gattttttct ttcccacgat
     1081 agcaaaatgc ggtcaacgta attaaaacta taagcctgat ttaatacggc ttcccgcagt
     1141 gctagcttca aaatttccgg ctgataatgg tcttcttcta gccattgtcc gatccgctgg
     1201 aactcgatcg aagaaagcgg gcgtccaaat tcttgttcaa ataattgata cactgactgg
     1261 atttcttttt cttgtgtttt ttcttcgtat tttttttctt gtgtttttaa atactcacct
     1321 aatagatcat agatggggta aagattatat cgatctgctt ttttaccgtt gacaatcgtc
     1381 gtttctatct tgataaatcc atttgttacg agacgatcca gtatttgaaa aatctgatct
     1441 tgtttgattc ccatatccat agcaatcatc tgcaaatcag ggaaagtatc tccttcaagc
     1501 tgtgccatat gcaactgtag aacaaataaa aattcttcag attgtagacc gatcctatga
     1561 taatttttaa gtaaaagatt agatatgact gtttgtccag cgcttaagta attttctaaa
     1621 ttcaacatga tttctcctcc gctcaatcac agtatacgta aaaaaaacat gggaagcaag
     1681 aacaggtttc ttttctattt acttcactga tcggtcagat ttacaatgtc gtctacttga
     1741 tttttgtttg ttcatgtttt tgatgcgtat cttcactttt cggaataaat aaattaagta
     1801 gatttcgctg ttccttagaa tgttgacaag gaaacaaaat tactatacac tatttttgtt
     1861 tccaagttaa cattattgca taaaggagtt tttacatgta cagtttcaca aataaacatt
     1921 ctaaagtttt tggatacgtc tctgtcttat tcactggatt aggatttact atcgtcagtc
     1981 ctgtacttcc gtttttaacg ctgccttatt ctcactccat acatcagcaa gctttctata
     2041 tcactttgct catgtctgtt tatgctctag ctgccttctt atctgccccc attctcggta
     2101 gcttaagtga tcattttggg cgtcatccga tccttattat cagtttatta ggatcaagta
     2161 tcggatattt gctttttggt ttaggaaatt cgatatggat gttgttcctt ggtcgtatca
     2221 ttgaaggatt gacggcagga gaaatctcca ccttatatgc ttactttgcc gacatcacgg
     2281 aacctaatga aagaaccaaa gtttttggat ggatgggtgc tttagtcggt ataggaacaa
     2341 cgctgggtcc tattataggg ggtctattag ctgagttggg gaattctgtt cctatattta
     2401 ttggagcact ctttactttc ttaaatgccg tttatggcta cacatttatg ctagaaagcc
     2461 ttccaataaa aaaacgttca gtagatttaa gtttctccca tgtaagacct tttcaccaat
     2521 tacaacaatt attcaagatt tcatccgtta ttcctttatt gacagcaggt tttgctgttt
     2581 ggttggcagc tggctctctt caatctatct tttctcaatt ttcgattgac gcctttcagt
     2641 ggaaggctgg gttggttggt ttaagcttct ctttgattgg aatcttggat actgtctcgc
     2701 aattgttcat tatgcctcga ttgctcaaaa aattttcaga acagcagatc acaagaatag
     2761 ggatgttcag tgaaatcctc gcctatctat tcatcacatt atctggaatt cttctactgc
     2821 cgattttgtt tttatttggg attatctgtt atggtttcgg tgattctatt tttactcctg
     2881 tattcaatgg ccaactatcc aattctgtct cagataacca gcaaggtctt gtaatgggcg
     2941 gtacccaaag tatacaatct ctatcgcgcg tcatcggccc gctaatcgca ggacagcttt
     3001 atgctgcagc accatttctt ccgacggtat tcggattttt gcttatgatt tttgcctatt
     3061 atcactacaa taaaaaatag tagcttcatt tgctacttcg tataaattct ctgtatcgac
     3121 cgataaaacg aaatactgtg tcaaattctt cttttgacat attttcaaat acagtagcat
     3181 ctcgctgtac ccatttttga tggagttgct cgtgtctatt ccttattttt tgtccgattg
     3241 gcgtcaattg aaaatagatt tcttttttat tctcttcgct ttgataagtg tttatgatcc
     3301 ctctagctat cagttttttt gttagcttac tgatggctcc tctagtcatg taactagcat
     3361 tcgctaattt tgtcacgtta ggatcaggga ttttcccgat atgttcaatc acttctagtt
     3421 catttagagt aaatcctgca aattcttttt ccatttctgg tttattacga tgaataatct
     3481 cattagctaa atccagatat tcttctatta gcagttgtct tttatccatt tctacacctt
     3541 ctttttcttt cattatattg tttttttgtt tcctattcaa caaagataaa aatattttgt
     3601 aggaaaaccc aatctgaaac caagaaaaaa aggctctata atttatggga ctgatgaatc
     3661 agaattgatt cttcagcccc atttttattt taggtattaa aaaacatatc gttctctagt
     3721 atgattaaat cacgacaaaa taaacaactg agaggacgat atgctatatg aatgattcta
     3781 tcaaaaaaat gctgcgatta atagagaaag atttgatgat tacagaggtc tcttacgaga
     3841 cctttcagaa gaaaaagacg ttgatcgtcg atgctgtttt ctcacctgct ccccatacct
     3901 gtagaaattg tggttctact gtggtagatg gaaacgggaa agt
//
LOCUS       DANKKG010000174         3925 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000174 DANKKG010000000
VERSION     DANKKG010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.176"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(573..1130)
                     /locus_tag="PW004_002592"
     CDS             complement(573..1130)
                     /locus_tag="PW004_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727520.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1309..1920)
                     /locus_tag="PW004_002593"
     CDS             complement(1309..1920)
                     /locus_tag="PW004_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727521.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2033..2290)
                     /locus_tag="PW004_002594"
     CDS             complement(2033..2290)
                     /locus_tag="PW004_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727522.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2887..3165)
                     /locus_tag="PW004_002595"
     CDS             complement(2887..3165)
                     /locus_tag="PW004_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727523.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3199..3450)
                     /locus_tag="PW004_002596"
     CDS             complement(3199..3450)
                     /locus_tag="PW004_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727524.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1195 a          799 c          520 g         1411 t
ORIGIN      
        1 ttattccgaa taaatggtgt ccaaaagctt gcatctgcca gattatctca tttcttatga
       61 caagctaagc aaattatctc caccaaataa tttattcata tttttgactt gcgcatacta
      121 actaattctt aaattttcat acaaaatttc tcaatttccc ttaacgagta atatttatgt
      181 acggtgattc tcactagtat cccaatcact ttttaagtta tcccgatttc aattctactt
      241 caaaaatagt tgcttttatg tatgctgtta gttgattaga tggtcatttg ttgagctagg
      301 tcgtttattt taaacaagtt gtttctttag cttataccta tctcgtctac caaacgcaag
      361 atggcaaaca caaatgcata cgcatctgta tttgccactt tgattcgtat aaagctaaca
      421 actattaact cctctcagct aatagttgct ctctttttta ttcagattca tttttgctga
      481 tcacagccta gtatttcaat ggttttgaac actaaaccaa attgttccat tacaacattc
      541 actggctgat gaaatggatt agtagatttc gattattcaa agcgattttc tattggtgga
      601 tcgtgttccc aactaataag acggattcca aacctatcgt acagatcttc taaaccactt
      661 ataaattttg cacctatttc aggtgtttta ttgcgtggat attctattgt tacgccatta
      721 aatggatacc aattaggatt aggctctctt tttttctgta catctcccat agaatagtta
      781 gcactttctg ggacataata tattcttcga ccatcctcta attcatctaa taagatattt
      841 cgtgatcgtc tatcacttgc taaatgtaga cgccacgtgc gataccatgc catgctacct
      901 gcatctgcag aaaatccatc aaataatgca acaattgtat cttctatcac aatcctttcc
      961 ccatctactt catattctaa tctaaaatca aactcccctc gggtaatttc tggtcttggt
     1021 ggcttaggac ccaatattat tctacttaaa accgcattcg ttcccagatg aaacacagct
     1081 ataacaacaa taatgattaa tattttgttt ctatttgata aatttctcat tattcgcaca
     1141 tcacctctac attctcacat attagttcgt aatactcctt taatatcaaa atatctactg
     1201 gtcgtaataa tattcgctat gaattcctac tactcgtata agaaaggcca aatcatcagc
     1261 ttttacttgc tgatgatttg gcttgtttct atttatcgta attgttttct aatcaccaaa
     1321 attgttaaca atcggttcag atatttccca gttgattaag gtaattccgt attgtgtcaa
     1381 aacttcttgc gcttctccga aattaagtac agttcttatg tatccacttt ctcctaatat
     1441 aaagtctacc tctcttttaa aaccttcctc gagatttcta agggctatat gtggttttaa
     1501 tgacattgat tgtacatttt cccccatata atattgcgca cttcctcgac taaaatagat
     1561 tcctagatcg tccccaaaag gtagatcttc tctaccacta gctaatctac ttctccatct
     1621 tcgttctctt ttcatagaac ctgcgctgaa ctcacacccg ttaaactcag ctatgacagt
     1681 atcttcaatt atgttaatct gtccatctag ttcgtattca atcctaaatg gaaattctcc
     1741 ataggtgatt tccggttcgg gtattttagg tgagattaat gcaaagaaag gaaatattaa
     1801 gcttggcaac atcaaaaaaa ctcctcctat gatcacaatt cccgatgtaa tccaaaatat
     1861 aatttgtaca attctcattg atccattgta ttccatttta ttccccctat tactattcat
     1921 atccgcctat ctatatcgat cttccaatgc agagataatt gcttccatac cttgattttt
     1981 tactgagtta cctatgcgag atatcattct ccactaaggt gcatcatatt gtctatctaa
     2041 catgattaat tctccaatag gtgtatactc ctccaaaaac aacatttaat gcatctccct
     2101 cgacaacata agctaccatg tttgctgtat atgtgtttga gtctaattca taagcagata
     2161 aatttactgt cgctggatta aatataatcg cattcgtatt tatagctact gaatttgctg
     2221 cttcagcacc accttttgaa tgtccgatca ttgtgatttc aatagtagga tttacgtgga
     2281 cagccctcac cgtttaccct gaccattata gaaatcgctt acttttttga actacgcaac
     2341 aaaggataca atgctcctaa cttcactgtc tccatttttg tccactaagg aatataaaaa
     2401 ataggcatta attcatttaa ttcggtcaca acacgttacc ttttaaatca ttatactgta
     2461 tctcatacat tattatcaat aaactattat aaaaatgcaa aaaatgcttt atcaatctat
     2521 tttgtaattg gtcataatat cattgaaatc ataccctgta tcgatttttc ctatgaatct
     2581 cgtctatttg tgattgacga aaaagaggca agacagacac tggaatggta attttaccca
     2641 ctattttgct ccaactattt gtaatcgtac ccacttttga aataccgaga tgatcatttt
     2701 aattgatatg ctgattccca agcaaaaccc caagaaatta aatgctctat cgcatctagc
     2761 ttcttgggtt ttgcttatat aaattccaac atttaaattt tttatgctgt tcggtctcag
     2821 aaatgccgtt tttgcttata tcacaatatc aagatgtcaa attattcgtg ttgccgatga
     2881 ctttgattaa tttttttcaa attgtttatt aagactaatg ctgtcttttt gcagatacca
     2941 atgttcgaac tttttaacat tatcatatga tcctgcaagt tttaacttct taccattaga
     3001 tgttatcaaa gttataatta accttgcctt tttcccatta ggatctttta ttttatgaat
     3061 tttctgcagg taatctctcc taatggcagc tatttttacc tcatcagtgc tgataaacca
     3121 ttgatcatcc acatacatac tgcctgttat gttgtgtttt tccatttctt ccgagaacga
     3181 aaagtttaag gctcgttctt acttccaaag ttgaatgagt ccatggattg gacttagtgt
     3241 gagaattgag aacaacaata agaatccata tcctcccgca aacatcccta aggctatact
     3301 gtctctccat ccataagtag gattagaaga gaaaagagtc accgtttgta ataatatgaa
     3361 aaggaataat ccagcacaaa caccaaagat aacaactcca aacattgaat ttttatacaa
     3421 tgaccattta tttataggct tttctttcat tttacttccc cctactgttc tgttcacacg
     3481 gaatgaattc aaagttcata agccctcctc tttgaacata tgaatacgac ttgctaacta
     3541 tactttaaaa aagcttgttt taagcggtta taggcgagtt gtaatctcta ttcatctcat
     3601 tagcaaataa cttgccgaaa agtctactga aattaaagca gatagtggaa gaattattta
     3661 gccaggatag acactatttg tgaaacacat gtctttactt ttgaaattct atgatgaaaa
     3721 gccttggaat acagtagctc ttaattggta atactttttc cattataaat aacctctttt
     3781 cattttatat ttatcccctc taaaaataat atctaatcta atattttaaa cttatatttc
     3841 cctcgctgtg ctaatagagc attactaaat aaatagaaac tagaaaaaag cattacgcct
     3901 gccagaataa accaattatt tgtat
//
LOCUS       DANKKG010000175         3759 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000175 DANKKG010000000
VERSION     DANKKG010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3759
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.177"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(27..1958)
                     /locus_tag="PW004_002597"
     CDS             complement(27..1958)
                     /locus_tag="PW004_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2727525.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(1976..2890)
                     /gene="pfkB"
                     /locus_tag="PW004_002598"
     CDS             complement(1976..2890)
                     /gene="pfkB"
                     /locus_tag="PW004_002598"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDL2727526.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(2887..3639)
                     /locus_tag="PW004_002599"
     CDS             complement(2887..3639)
                     /locus_tag="PW004_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL2727527.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
BASE COUNT         1036 a          855 c          666 g         1202 t
ORIGIN      
        1 aaaacccagc ctcttctttt ttatttctat gcttctgcta ctgcaggagc ttttctcaat
       61 aaaccataaa tcacaccaga tacgactgct ccgatagcaa taaacaccaa atacatgacc
      121 gggtgactca gtaagaatac aacgaaaatc ccgccgtgag gagccagtaa tttgattccg
      181 aaagctccga ctaatccgcc agttaaagca gaaccaataa taaaacttgg aatggcacga
      241 atcgggtcag ctgctgcaaa tggaatcgat ccttccgtga caaacgatag tcccatcacg
      301 atgttcgtca aaccagcatc ttgatctgtt ttgctgaatt tattcttaaa caaacgtgta
      361 gcaacgaaaa ttgccaatgg aggtaccatt cctccagcca taacagaagc catcacgaca
      421 cttccgccag ttgcaacagt cgtagctaaa gttgctgtac caaacacata agcggctttg
      481 ttgattggac cgcctaaatc tgccgccatc attccagcta ataatgcacc aagtaaagca
      541 gcattcgaac cactcatact tgtcaagaaa ccgttcaatg catcattgat tgctttcata
      601 gggacattga ccagcaacat caagaaacca gtaatcagta agccaaacac tggataaaag
      661 aggatcgttt taattccatc caatgatttt ggtaagttct tgaataattt acgtaaaaat
      721 acgatgacat aaccagctaa gaatccacca atcagtgcac ctaagaatcc tgcaccgcct
      781 gtattagcta atgcaccagc agcaaatcca acgatcaaac cagggcggtc accgatactt
      841 gaagcaataa atcctgctaa taccggcagc atgaaaccaa atgcagcttg tccgatttgg
      901 ttgaaccagc tagcagcttg attgtaattc cctagatttg ataattgatc atgtggcacg
      961 ccaataaatt ggtcgatcat gaaagaaagc gcaatagcga ttcctccacc aataacgaaa
     1021 ggtaacatgt gtgatacgcc gttcatcaaa tctttgtaaa ttctttgtcc gatagtacca
     1081 tccgcatttc cttcttctga actgctttct tttccatcac catggaatac tggtgcagta
     1141 ccactaaggg ctagattgat caactcttct ggtttacgga taccatcact gacaggacga
     1201 ttgatcagtt cttttccgtc aaagcgattc atctctactt ttttgtctgc tgcaacgatt
     1261 acaccttctg cacgggcgat gtcttcagca gtcaatcggt ttttgattcc ttctgaaccg
     1321 ttcgtttcga ctttgatctc cacacccatt tctttcgctt ttttcttcaa agcatcttct
     1381 gccatgtaag tgtgagcgat ccctgttgga caagctgtaa cggcaacaac gaattttctt
     1441 tctggatgag cagaagcttc tgcagcttgt gtttcttgtt ctgctttttc ttctgcttct
     1501 ttttgctctt cagctgtttg gaacaagtct tggacttctt ctggtgtatt tgcttctttt
     1561 aattttccta cgaaatctgc atcaatcagc aatcgagata gtgctgctaa tgcttgcaaa
     1621 tgtgtgtcgt ttgcgccttc tggagctgcg atcatgaaaa acagataagt tggctgtccg
     1681 tctaaggctt cataatctac tcctttttgg ctttttgcga ataaaactgt tgcttcttta
     1741 actgcactgt ttttcgcgtg aggcatggcg attccatcac caagacctgt agaagtttgt
     1801 gcttcgcggg ctagaattcc ttctttatat gtttcgatat cggaaatccg gccggcatca
     1861 aacatttttt ggaccatttc atcgattgcg ccttttttat cagtcgcttg caaatccatg
     1921 atcatcgctt ctttgaccat taaatccttg atattcatga ttatcctcct tgaaactaat
     1981 caatttttgt gatttcaact tctggcagca actcatcaat caattcgcga gctgccaagt
     2041 catcagaaaa agctgttgcg cttccgcaag caactcccca tttaaaggct tcgattggat
     2101 ctttcgtacg gctgtaagcc ccaataaagc cagcaatcat tgaatcccct gcacctactg
     2161 aatttttcaa tgggcgaact aatacattcg ttttataagt tccttgttcc gtgaagagca
     2221 aagcaccgtc tccagccatt gagatcagca cattctgtgc cccttcagct agtaattttt
     2281 tcccatacgg aatgatttct tctacagatt ggaaggttgt gttgtaaagc tcagctaatt
     2341 cgtgattgtt tggtttgatc aacaacggtt tcttaacaag tgcatttttt aggtcatcac
     2401 ctgttgtatc aatcacgaaa tctgcacctt tttctcgaat gatatcaatc agttcttgat
     2461 aaaatccttt tcgcaggcta gctggcgcgc ttcctgacaa tacgacgata tctccttgtc
     2521 ctactgttcc taatgcttgc ttcaactcat cgatttcttc atctgaaata gatgggccta
     2581 agccattgat ctcagtttca gtgtctgatt ttagtttgat attgattctt gtatcttctt
     2641 ttacggttgt gaacaaagac tggatttgtt ctttttcaag ccacttcgaa ataaattcgc
     2701 cagtgaaccc tcctaaaaac cctagtgctg tagattgaga atcgatccgt ttcaaaatac
     2761 gggaaacatt gatccctttt cctccaggta acttgaagtc ttccttcatt cggttcaaat
     2821 cgcctagttt taacccatct acgcgtacga tataatcaat tgatggatta agtgttactg
     2881 tgtaaatcat tgtattgcct ccttaattgt tgtcttttca cggaattcat taacgaattc
     2941 taatggacaa cgctcggtta tgatcgtggc tgcttccaaa ggtgcaacag aggtgaagga
     3001 caactggttg aatttggtat gatcaatcaa tacaaatgtc tcttcggaat ttttcatggc
     3061 taaacgttta acagctgcct cttctggatc cggagtcgtg aatccttgtt caagatgggc
     3121 accgttcatt cccataaatg ctttattaaa atgaaaattt tttagttgtt ctatcgcatt
     3181 agatcctaaa acagcattag ttgccagctt tatcgttcca cctaaaataa tggtagcaag
     3241 ctccaaatca actaaatttg ccgcatgttg aacggaattt gtcactactg tgatctgttt
     3301 tccttgcaga aaaggaatca tttctaacgt agtagagcca gcgtctaaat aaatgacatc
     3361 ttcgtcatta atggtttcag ctgccgattt ggcgatttgc tgtttttcgt gaacgttttt
     3421 gagtgatttt tcactcatat tctgctcaaa attcaataat tgctctttct tagctccgcc
     3481 atgaactctt tctagaaacc cactattctc cagctcttgt aaatcacgtc ttatcgttga
     3541 ttcagaagca ttcaaaaggt ttacaagatc ttgtgatttc acgattttct gctgttcaag
     3601 caagtctagg attttttgtc tgcgttcctc tgtaagcatt ttcacgatca cctcaataca
     3661 aataatagca tatttttttt ctactagcaa tataaatcag tcaaaaacgt tcataaaaat
     3721 tataaatcag tcaaaaaaaa aataaaaaac cagtcagaa
//
LOCUS       DANKKG010000176         3753 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000176 DANKKG010000000
VERSION     DANKKG010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3753
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.178"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..103
                     /locus_tag="PW004_002600"
     CDS             <1..103
                     /locus_tag="PW004_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HDL2727528.1"
                     /translation="LESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
     gene            complement(162..1964)
                     /locus_tag="PW004_002601"
     CDS             complement(162..1964)
                     /locus_tag="PW004_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HDL2727529.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFLGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYCWKYKD"
     gene            complement(2027..2869)
                     /locus_tag="PW004_002602"
     CDS             complement(2027..2869)
                     /locus_tag="PW004_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727530.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            complement(2885..3106)
                     /locus_tag="PW004_002603"
     CDS             complement(2885..3106)
                     /locus_tag="PW004_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2727531.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            complement(3249..3554)
                     /locus_tag="PW004_002604"
     CDS             complement(3249..3554)
                     /locus_tag="PW004_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HDL2727532.1"
                     /translation="MNNLLEKKQKELIKLANKGQDYEHLADEIDELRDKRQLLLVEDA
                     SLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
     gene            3654..>3753
                     /locus_tag="PW004_002605"
     CDS             3654..>3753
                     /locus_tag="PW004_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDL2727533.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNISYREVQE"
BASE COUNT         1113 a          749 c          541 g         1350 t
ORIGIN      
        1 gctagagagt agatttgaaa gcatattact ggcttacagt gttctattaa gtcgagcaca
       61 acgacgtttt gaaggaactt tgagatattc tttaggatat taaaattaac tagcaccacg
      121 ggtatctatt aagaagatcc taggttatct tagattatct gctaatcttt gtatttccag
      181 cagtatcctc cagcatgttt ttgaactcca tttgcagcat ccctaatact ttttgagtta
      241 acgccaactt cttttacagc agaagctata gtatcgaatt cttttattat tgttccatca
      301 tcagtcattt gaataatctg tcttccacgc ttcttgctag gctcactaat cgcatcctct
      361 tttttgttcg cttttagaaa atcagatttt tccttagcta tccgctctgc ctcttgttta
      421 gcaaataata gcttaattgc cttatcccta cgagcctcta aagtaccaac atcgtgccgt
      481 gttttacgca taattccttc atcaagaatg agaatttcat tatcgtaatc cttaattttt
      541 cgataagctt tagcataaga atcatatagc gcaggagcat catagccgtt atatcgagct
      601 ttatcaaaaa gtgcaatagc ttcatctact ttgccatttt tcctagcagt ttctccacgt
      661 tcccaatatg gataacctgc atcaaatcct ttctcccaat tccctatatt tcgtatcttg
      721 tttaatgaaa ctcgattatg caacccgtta ataattacat cttctctaga tatcactttt
      781 tcaatattta ggttaacttc agtctcatca tatccacgat tttttgcatc gaataataac
      841 tctttcatcg ctgattttga aatagatttc agcatactaa ggctttgctc tgcttctttt
      901 ttagtgtaat tactaacact catatattca ttcatagaat tataacaatc gctataagca
      961 ctgaaaatat aatttgaaag aggcgttaag tcaaaggggc tacgaaaata gactcgcaca
     1021 tttgttgtac cgccttcact tacagtacaa ggttctatag gtaaatcaat tccatttgta
     1081 gcatcttttc taataatgat gtaatttccc ttcttagcaa acttaaactt taagaatgta
     1141 tatagacaag ttacatcgac ataattatta ctgttttttc gatacctgat ccaactggtg
     1201 tcgcatcctt ttttctcaat aatatcttga ataaaagcac aagcttcaag ttcttttatg
     1261 tctggagtca tcttttctat ttgctttttt tgttcttcca acgactcctc aataatatct
     1321 aaaacaccca aaaaaatctt tccgcatata tctgcatctg attccgctct atgggcagtg
     1381 tcatttatta atccgaaata ttccgctaca gtctcttgct tatgatttac aatgcctttt
     1441 atatgttttc tagagagact cagagtatca acatacttaa ttttagcatc atacccaagt
     1501 ctagaaaatg tgttacttaa aaatccaaaa tcgaaacttg cattgtgtgc acacatagtg
     1561 atttttccat ctagtgcatc tcctaaaaat tctaccaact gggggtaaac ttcctgctca
     1621 tttggagcag agtttagcat ttcattagtt atattattta ttgccgaagc tgaggctgca
     1681 attctaactt ttggattgac aagagttccg aatgattgaa ttggaactcc ttctttaaat
     1741 agcaccgcac caatctctac aattctatct gaagaagaat ttaggccagt tgtttccaca
     1801 tcaaaagcga taaaagtttt acgaaactca tctacatttg atatgtccac ttccaatttt
     1861 tcagcaaaaa ctttaccagt acgaaataga ctagaagcca gttgataatc aacgtgttta
     1921 ctaacctttc cttgtttttt ctttagaaaa tcaaataatc ccactattat ttcctccccc
     1981 attaatttta tccataatca cttttacgat gtctttttct tatattctac ctattcgcat
     2041 accacaagaa gtgatctaac tttcttcttc taaaaggaat atgtatatta ttttcatcta
     2101 gtagttttcc aacacattcg ttaaatgcct cgtaatcaaa acttcttcgc cacgtatcaa
     2161 tcttattcgt agttagtcct tggatatagt gtggaagagt atttttaacg ataccatcaa
     2221 agatggagta atcatctctc ccacacactt ccacattatg atatgtacag tacttggatg
     2281 caaatgagaa catattaact gcaccaatgt tccttgcgat aatatttatc aactgaggat
     2341 ctccttttgc caaccgatca tcaaaatttg gaatctccag aattacttta gccaaatcat
     2401 ataaagacaa cctgctctta tattgagaaa gatgtgttga atttgttaca tcaattaccg
     2461 caatcttcat cgcaatagta tttaaatcat tatgaagagg aaatgcattc aaaacatcat
     2521 gtattattag accttctttc ccataattag agcttgcacc tacaaactta tgctgtttct
     2581 caatattttc ttcagttaaa gataagtatt tttgtttatg gattgtttca cttatagttt
     2641 tttctttact tgaatcaagc ttaagaacct tgtcaatgca ccaatatgtt gttgctcctt
     2701 ttattgggga aatatcattc tgcttaagat attcttttaa taaactcagt tgttttcctt
     2761 ctggaatagt accagatttc gtttctatga tatgaggttg tggtttggaa gtaccttcat
     2821 tagcagcaaa tacagcatac tcaacatcat ttattgttat gattttcaat acaaattaca
     2881 cctcctaatt acctatcttt atttcattgc catcaggcaa tataaaagcc tgcacaaact
     2941 tgacacctag cacatcagca atcgctttca actcatctaa ggttaccgtt tctcgttgta
     3001 atttcttatt gaaattctgt ggagtctgtc caatacgtct agcaagttcg gaaacactta
     3061 tattcatttg ctcacacagt tctttaatca tttctgccgt agtcatacag cacctccaag
     3121 tttacgctaa gaatattata aatcatttgg tttataataa caatagttat taaaatatta
     3181 ttataatcag caagcaaaag aaaacccacg actctactgt agagtcatgg gctatttact
     3241 tctggttttc atatttccat ttcgatgcca gatttgaagg atatgacgaa gtgttcttca
     3301 tagactgtaa cgctctggat tatcttcctt acaagcttat catcgtactc taaggtacgg
     3361 aatttgttct tgcggataaa ttcaatcagc tcattgattc gctcgttctc gccactgagg
     3421 gaggcatctt ctactaaaag gagctgtcgc ttgtctctca gctcatcaat ctcatctgct
     3481 agatgttcat aatcttgacc cttattagcc agcttgatga gttctttttg ctttttctcc
     3541 agtaagttgt taatctctga aatttggtat tctgtcgttg gttctgttgc aaagttttaa
     3601 atctactatc aaataaggta gaataataga aaaagatagc agaaggaatg acgatgaatc
     3661 attttaaagg aaagcaattt cagcaggatg tgattattgt agccgtgggc tactatcttc
     3721 gttataacat tagctatcgt gaagttcaag aaa
//
LOCUS       DANKKG010000177         3744 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000177 DANKKG010000000
VERSION     DANKKG010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.179"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(174..338)
                     /locus_tag="PW004_002606"
     CDS             complement(174..338)
                     /locus_tag="PW004_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727534.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            633..1961
                     /locus_tag="PW004_002607"
     CDS             633..1961
                     /locus_tag="PW004_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HDL2727535.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2163..2996
                     /locus_tag="PW004_002608"
     CDS             2163..2996
                     /locus_tag="PW004_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HDL2727536.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            3012..>3744
                     /locus_tag="PW004_002609"
     CDS             3012..>3744
                     /locus_tag="PW004_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HDL2727537.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKN"
BASE COUNT         1245 a          591 c          780 g         1128 t
ORIGIN      
        1 atcatgatgt cgtaggttcg agtcctactg ccggagtaaa taaagcttat gcaaagaaaa
       61 agaaatccaa tcacttgggt ttctttttct tttattatta tgatgaatca agctttctgt
      121 ttttgtcacg tatgttgtaa tctttacgaa agagtagagt tggctactca taatcaacca
      181 tacttcccat catcgctaaa tacgaaataa atcagcattc ctacaccaat caaagcacaa
      241 gctgcaccaa gaaaagggat gacattccca aagttatctg tattcataag tatgccacca
      301 cccacaaaca aaaagccgcc agcaatcagg tttttcattt tattcatctc ctttcttacg
      361 ttatagtatt tccttctttc aatgagtatt ttgaaagcta tcttattgta tgatgttctt
      421 ctaggcctct tctttattat acaggccatt ttcgttatct catagaaata ttccttttta
      481 tctgaaaact attgtatacc gtatgacatt tttcttcatg aagttgtaag tatattctca
      541 ttcaaatgaa aatttcatca tagaagagat tgtatttcta aattcttaag agtaaaatac
      601 gaagtataac ttggatttgg aaggatgatt ggatgaaagc tattacgcct gatatgacaa
      661 caaaatttaa caaacagttt caagcaaata tcaaacagca ggcattgcag cgggcagttg
      721 tgaagaacgg aattactgct tctgctgaaa atgtttcagc acatgtggag aatacaccgg
      781 tattttctat agatttggct acggggaaag tggctaatca aatgcaatcg gggcgttgct
      841 ggatgtttgc tgcactgaac acattccgtc ataagatgct gaatcaattc catttaaaag
      901 agtttgaatt atcacaaaat tacacttttt tctgggataa atatgaaaag gcaaattatt
      961 tctatgaaaa catcattgca acaggaaaag aggcaatcga tagccggaaa gtagctttct
     1021 tactcgctac tcctcaacaa gatggcggcc aatgggatat gatcgtttca ttattccaaa
     1081 aatatggcgt tgtgcctaaa tcggtgatgc cggaaagtgc gaatagttca aattctcgag
     1141 atttgaataa ctacttgaac aaattactgc gtaaagatgc agtagtgtta cggaaaatgg
     1201 ttgctcaagg agaaacatta gacgctattg aagaaaagaa agaagaaatg ctagaaagca
     1261 tttataactt cttagctatc tcattaggaa cacctccaaa agaattcgat tttgagtacc
     1321 gcgatgaaga aaagaactat catcttgacc gaggactgac accacaaatt ttttatgata
     1381 aatacatcgg tgtaaaatta gatgattatg ttagcgtgat caatgcacca acaaaagaca
     1441 aaccattcaa tcgttcttat acagtagaga tgctaggaaa tgtcgttggc gggaaagaag
     1501 tcaaatatct gaatgttgat atggaaactt tcaaaaaatt agcgattgct cagcttgaag
     1561 aaggagaatc tgtttggttt ggttgtgatg taggtcaatc ttctactaga actagtggca
     1621 tcatggcctt agatgcttat tcaatggatg atctatttga tacggatttt acaatgacta
     1681 aagctgaacg tttagatttt ggagaaagtc tgatgaccca tgcgatggta ttgaccggtg
     1741 ttgacttagt agacgggcaa tcaacaaaat ggaaagtcga aaatagctgg ggcgaaaaag
     1801 ttggaaagaa tggcttcttt gttatgagtg atgcctggat ggacgaatat acgtaccaaa
     1861 tcgttgtaag aaaagattta ttgacgaaag agcaattaaa tgcgttcaat gaagaaccaa
     1921 ttgttctatc tccgtgggat ccaatgggtg ctttagcata gacgagtatt tcaatgaaga
     1981 gtatgtagta aagataaaaa gagctggctt aagccagctc tttttatctt taaagtccat
     2041 tccctgtttt ttatggtccc tgtgctataa ttctaaataa aggataagga ttggaaataa
     2101 tccttttatc attaaaaata tgatgacgaa gtagaataaa attgggagta ggggggaaag
     2161 tcatggatat ccaaactgtt ttatctgatc tgaaattaaa tggtaaagtg attccagttg
     2221 ttggtggcga tgttaatcag acctatcgta taaaaaccga acatcgtgct tattttttaa
     2281 aaatccatcc gaatgtaaaa aaaggatttt ttgaagcaga agtagacgga ttaaaagaac
     2341 tgtcagcatt cgtacgtgta ccggatacgt atatgctagg tgaaacaagt gaaggcgctt
     2401 atttgttaat ggaatggatc gagccgggaa aaggggatca acgagatctt gctgcagctt
     2461 tagctaatct ccatcagcaa acagcgccac aatttggttt tcgtaaagat aactatttgg
     2521 gtacacttgt tcagaaaaat tcttttgaag aagattggtg gacttttttc tttaaagatc
     2581 ggcttgaatc ccagatttct ttggcagaag aaacgaatcg atggaatgta cagcgtcaag
     2641 aaaagtatct gcgatttaaa gaacgtgtat tgaaatcggt tgaaccaaaa aagatcactc
     2701 cacgcctctt gcacggagac ctgtggagtg gtaatgtgtt ctttgaccag caagggcatc
     2761 cagtttttgt cgacccagct gtttcttatg ggaatcggga acaagatatt gcaatgagcc
     2821 agttattcgg tggtttccga ccggaatttt tagatgctta tcaaactata tttccattag
     2881 aaaagggatg gaaagaccga ctgccgattt accagttata ttatcttttg gcacatttga
     2941 atatgtttgg agaaagttac ggttcccaag tagatcaatt actagaaaat ttttaagagg
     3001 agggcgtgtt tttggcaaaa aaatatacca aaaagcatga agtttcatat tatgagtgtg
     3061 acatcaacca aacgatgaca tttccttcga tgttaggagt agttatcaaa acgtctgaag
     3121 atcaaagcga ccagttggga agaggcagtg aattcgtcaa tagctttgga cttacttggg
     3181 tcatcacgaa ttattccatg gagattaatc gtctccctag agtcggtgaa gtaatctcag
     3241 taacgactca agccatggag tacaataagt ttttctgtta tcggaacttc tggatccatg
     3301 atgaagatgg gaaagaatta gtcaaaatag aatctgtttt cgtattgatg gattttgtga
     3361 atcgaaaaat gagcagtgtt aatgaagaga tcattgctcc ttttgagagt gagaagatca
     3421 agaaaatcaa gcgtcaggaa aaaattgaaa agattgattc gggcttcatg ttgccatatc
     3481 gtgttcgttt ttatgatatc gatagtaatc agcatgttaa taatgcgatg tattttaatt
     3541 ggatcattga cgtattagga tatgactttt tgactacgca tgttccagaa aaagtaatta
     3601 ttcgatttga taaagaagta gagtacggga atgagatcga aagccatttt gaacaaatcc
     3661 ctatcgaaaa tggtgtcaaa acaaaacatg aaatccgaat gcaagaacag ctgtattgtg
     3721 aagcaaatat tatatggaaa aata
//
LOCUS       DANKKG010000178         3738 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000178 DANKKG010000000
VERSION     DANKKG010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.180"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..445
                     /locus_tag="PW004_002610"
     CDS             <1..445
                     /locus_tag="PW004_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDL2727538.1"
                     /translation="KEKQMEEQDFKRFLAMIDECHNAINPDFIETVNYVTNFQREMRK
                     VEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEGLVGKS
                     MTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            445..1644
                     /locus_tag="PW004_002611"
     CDS             445..1644
                     /locus_tag="PW004_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDL2727539.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWVFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYKLL
                     PSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKFPN
                     SKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSAYYKNCMSII
                     NTNKLMEYDEFAIKHWREGGGTGGTITGSWGNPFPGSSLDKNSFSGGQLFGKNPGGEF
                     RPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVYQE
                     FANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDPLP
                     FLNSSKK"
     gene            1660..2304
                     /locus_tag="PW004_002612"
     CDS             1660..2304
                     /locus_tag="PW004_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727540.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTYKIEEVAK
                     NASDKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            2315..3121
                     /locus_tag="PW004_002613"
     CDS             2315..3121
                     /locus_tag="PW004_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727541.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFMILGFLILASPIYTHKKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFVVKLAHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQMKDQEEIIQKLQEKIDRMTKEKI"
     gene            3143..3373
                     /locus_tag="PW004_002614"
     CDS             3143..3373
                     /locus_tag="PW004_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727542.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
BASE COUNT         1384 a          582 c          779 g          993 t
ORIGIN      
        1 taaagaaaaa caaatggaag aacaagactt caaacgcttc ttggcgatga ttgatgagtg
       61 ccataatgcg attaatccag attttattga aacggtaaat tatgtcacaa actttcaacg
      121 agaaatgcgt aaagtagaag caatgactgt tttagctaca caatccccac aagaaatggt
      181 gccagaaaat atgtcttctg ataacttctc gaaactgaag aaagtctttg aattttcttc
      241 tacgaagatc tttatgcgta tggatgaatc tattttacgt catatagaag gattagtggg
      301 taaatcaatg acgaactcag aattagaatc cattcctcaa caaaatcaag gagacgctgt
      361 gatcaatttt ggctcaaaag aaaccattcg tgttcatttt agtccgaaca aacgtcaatt
      421 aaaattgttt gatggaggaa aataatggaa aaccaaaatg aaagtttaat caaacagtat
      481 gttaagcgtc gtgcaaagcg gcgcttattt ttgtgggtgt ttggtacttc tgcaggtttg
      541 attactatat tgatcactgt gtttgtgacc ctttttctaa tacttgcggc aggttctatt
      601 gataattctg attcaagtag tggtggcgaa gcttttacag gagaatattc ggaaggctta
      661 cccatttata aagaaatcaa aggaagaggc cctttttctg atgagattgc gcaatatgct
      721 gtaggtgcag cagtgaagta taaattatta ccaagtgtga tcctttcgca atacggttat
      781 gaatcagcgt ttggtacgtc ggcgtccgca agaaatgatc tcaattattt tgggattacg
      841 tggtttgatg ggtgtctgtt tcctaaagga actgcgcgtg gaattggtgg cattgaaggt
      901 ggctggtaca tgaagtttcc caactcaaaa gctgccttct cctattacgg ctttatggta
      961 gccacacaat caaattttaa tgcttgtgtc ggaaataaaa gccctggcgc aagcttattg
     1021 atcttaggac gaggagggta tgccgccgca ggaattactg aagacagtgc ctattataaa
     1081 aattgtatgt ctattattaa tacaaataaa ttgatggagt acgatgagtt tgcgattaaa
     1141 cactggagag aaggcggcgg aactggtggt acgattacag gaagctgggg caatccattc
     1201 ccaggaagta gcttagataa aaattcgttc agtggaggac aactttttgg gaaaaatcca
     1261 ggaggagaat tccgacctaa cgggttccat gatggactag actttggttc agtcgatcat
     1321 ccaggaagtg agattcatgc ggttcatggc ggaaaagtcg tgtacgttgg caatcctggt
     1381 atttcaggtt tgggtgcgtg tgtgatcgtg attaattacg atggcttgaa tatggtgtat
     1441 caagaatttg caaatagtac aggaaattct cgagtgaaag taggcgatca agtgaaagtt
     1501 ggtcaagtaa ttgggatacg agatacagca cacttgcatt taggatttac tcgaatggat
     1561 tggcgtcaag cacaaggaca tgcattcatt gatgatggta catggatcga tccgttacca
     1621 tttttgaata gcagcaaaaa atagatcagg agaaaaaata tggaaaataa aaccttaaaa
     1681 atcgtcttaa cagctgcaat tagcgttagt ttaatcggca atattgcgtt gttttggcag
     1741 cttaaagaag aacaagagac aaatacctat aaaattgaag aagtggctaa aaacgcgtct
     1801 gataagctca aaaaaaattc ttctgaggca caggcgaaga tcgacaagtt aaatcaaacc
     1861 atcaatgaga aaaaccaaga aatcgatcag ctgaagcaac aagatggcac aaaagcaaaa
     1921 gaggaagtaa ctgaagcaca aagaaagacg gcagaagagt tcggtcaatt ggccattgat
     1981 aaatcattaa gcagaaatga attaacggaa aaactaaaag atgtcgcaac ccaagaagtg
     2041 atcgacaaat taagtcctgc agacgatgat caccaacatg acgactacgg aagttactct
     2101 tttacattag gtgacgtgca aacttacgct caaacaagca gtgtaaaaga ggaacaaaat
     2161 tttgtcgtat ttgttgatta tacaatcggc aatcctcaat ttaaagatgt caaaccacaa
     2221 aaaataaaag gtggattaac tgtcactgaa aaacaagtag atggtcaatg gaaagtcaca
     2281 gactttagtt atttcactcg ttaggaggaa gaaaatgagt aagaaaaaag gagcaagaca
     2341 acgttttaaa aaacgaaaaa gttatcttag atggctgggg atctttatga ttctcggctt
     2401 tttgattctt gctagtccga tttacacaca taaaaaggta gacataccag atgaagtaag
     2461 cacgaacgca tttttaaaca cgaaggaact gacgatggtt tcaaaagaaa aaggagcgaa
     2521 ccagcaatta gtttttgaat ttgcgatcga cgatgaagat cagagcttat taaaagaact
     2581 agctaatctt aattatcgtg tagaagtaaa gacagctaaa ggggattatc aagcaataaa
     2641 agccaaagtg atcaaggtgt ctgatgatta ttttgtagtg aagttagcac atgtaccaga
     2701 agaatatatc gccatgcgtt taaccattat tcctgaaaaa attgatccaa aagtggatat
     2761 gcaagaacca caagatctga tttattacgt tcacgaagat aaggttaaag atcaagtgaa
     2821 agatgaagat tatgaacaac atgcgatcaa ttacaaagtg aaaggataca aaaaagaaca
     2881 aaaagcagca caaaaacaaa tggaatcctt ccaagcaacc attgatttga atgaagaatt
     2941 agtgaagaag ctaaaagatc aattgcctta tcaagtcgca gatgatcagg aaaatactga
     3001 aaacaagatt aatagctacc agcaagaaat tgaaacatcg aaaacgcaaa tgaaagatca
     3061 agaagagatc attcaaaaac ttcaagaaaa aattgatcgt atgacaaaag aaaagattta
     3121 gaaggacagg tggagtgaaa aaatgaagcg aacaaacttg gctgacaaag atactttcgc
     3181 tgagcagaaa gtattgatta aagcctatct caaagttgta aaagctaaag aaatatgtcg
     3241 agaagtgaat ttatctaata caacaatatc tgagttgaag aatggtcgaa agaatttaga
     3301 tactttaaaa ttcagatcat ttgaagctct atatgtagct gctaaaaaat attttacagc
     3361 tgataacaaa taaaaaacag gagtatttta tgtgaaaaaa gtatataaaa aatgcttatg
     3421 aatcagctat atcaccttaa ccatttgtta atgagatggg agaaaacatt aaccacgatt
     3481 ctaacgcaaa catcaagcac ataaaacatt taacactcct cctttctggc tgaagatttc
     3541 aaccaaacag gaagacagcg tatcaattat agcatacact ttttcttaaa ttcatttttc
     3601 atatgacaaa atatttcctt ctaatcgaca aagttagaag gattttttat tgtctaaata
     3661 cacaaataac cttaatttat aaagagcata aggaaacgtg ggaacacttt ttaaaaaaag
     3721 tgttccaata ggctgtgc
//
LOCUS       DANKKG010000179         3727 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000179 DANKKG010000000
VERSION     DANKKG010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3727
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.181"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            109..957
                     /locus_tag="PW004_002615"
     CDS             109..957
                     /locus_tag="PW004_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL2727543.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAVLGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFVFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            1057..1491
                     /locus_tag="PW004_002616"
     CDS             1057..1491
                     /locus_tag="PW004_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL2727544.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            1672..2082
                     /locus_tag="PW004_002617"
     CDS             1672..2082
                     /locus_tag="PW004_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HDL2727545.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            2139..2579
                     /locus_tag="PW004_002618"
     CDS             2139..2579
                     /locus_tag="PW004_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL2727546.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            2688..>3727
                     /locus_tag="PW004_002619"
     CDS             2688..>3727
                     /locus_tag="PW004_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL2727547.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVIALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVK"
BASE COUNT         1109 a          606 c          705 g         1307 t
ORIGIN      
        1 ataataattt agactagcct aattttttta ttaattaaag ttaacttaag gtgaaaagta
       61 agttataata agataagagt attattaatg aattaaggaa gtggataaat gaaagaaacg
      121 agacaattta tatggacata cattaagatt atttttgccc tcttcatatt aggagtcgct
      181 atcaatatgt ttttagggcc tcatcatatt gctgctggag gtgtcagcgg tctaggaata
      241 ttactggaag ctgttttagg atttgaccga gctatagtta ttttagtgtt gaatatcgtt
      301 atgttggtac tagctcttct atttttaggt aaaaaaccct tttttaaagt acttttcgga
      361 agcttgtttt ttcctttaat cattgcaata gttccagaaa caatgattac ttcagatcgg
      421 ttgttgtctg tcatttttgg aagtgcgatt tttgctttag gagttgcgat tttatataaa
      481 aacaactctt ctagcggcgg tacgacgatc cctcccctca tttttaaaaa atatttccac
      541 ttgaatactt cgattggctt attttttaca gacgcattcg ttgtttctct taatctgttt
      601 gtttttggaa tagaagagtt tctttatgct attttatcta ttgcaatcac ttcaattgtc
      661 atgacttata tcgaaacagg tatcaatcga aagaaatctg ttatggtcat gagtgaacat
      721 tatttggaag acatccgcct tcgcttaagt gctgagattg gacgtggcct taccctgcta
      781 gaggcaaaag gcgggtataa tcgaaagtca aaagaggttt tattgatcgt tgtgactgat
      841 catgaatttt cccgaatgaa accattgata gaagaaatcg atccaagtgc ctttgtcatt
      901 gtcagcagcg tagcagaagt aatgggacgc ggatttactt atcatccaat cgaatgagtt
      961 ggtaagtaaa tccgctattt ttttgaatgc gttttcttta actggtataa taaaagaatc
     1021 tagtaactgt tcgactgaaa aggagaggag atctttatgc ataaaggaac ggaaatagat
     1081 tttgtattaa atgatagttt gaaagcacta gaactgtatc aaacaatctt tgatgaaata
     1141 caactgattg aggcaacaga ttaccctaga ggcaataatg aagtcatttt taccttgtat
     1201 ggcacgagat tccatatgct ggatgaaaac acgtctcttg gtttgactgc tcctactgct
     1261 gaacatccaa atacgatctg gttcaatatc atcgtctctg atatcgagga cacacataat
     1321 aaagcattac gaaatggctg tgtagagatt caagaaatga cagaaatgat ggactttggt
     1381 gtgactaact caatgttcag agatcctttt ggttatttat ggatgcttca tcaggtccat
     1441 cgtgaagtaa gttttgaaga aagaaaacaa ctttttgaga aatatatgta atgtttatga
     1501 tgagattagt ttttgtctct gatatttttt tgtatatgtt tgctattcga gaaagcgcgt
     1561 ctgtttcatg tgctttctct tatttatctt atgccaatcc atttctttgc tttccacaat
     1621 tatattgtgt aaaattcaat tgtataaaat ataattaaag gtggtaattt tatgaaaaaa
     1681 atgtattcta cgaaaatgat caacacaggc ggacgctctg gagaagttca taatcccgat
     1741 cgtacattcg aattgaatat tgcagctcct ggtaaacgcg tcgaaaatgc aaccaaccct
     1801 gaacaattgt ttgcagctgg tttcagtgca tgttttaaca gtgcattaga attgatcaaa
     1861 cagcaaaaaa atatctctgg ggcttctact gtcagtgcac aagtttccct ttattcagaa
     1921 agtgaaacga gctttgtctt aggtgtagag attgaaggat ctatcgaagg tctttctctg
     1981 gaagaaacgc aagaattatt agaggcagcg cataaagtgt gtccgtattc aaaagctaca
     2041 gctggaaata tagaagttac tctgaaagca gttagtgaat agactttttt gtgtaaatcc
     2101 gctatgatac actatagtaa aacagcggag gtggaaaaat ggaagatcta tatttagtta
     2161 atcaagtatg ttttccttta tatgccgcct ctaaggaaat cattagacac tacacttccc
     2221 tcctcaagcc gctgaatctt acctataccc agtatcttgt gatgttagtt ttatgggaaa
     2281 agaaaaagac ttcagtgaaa gagctaggaa atgatttgtg tctggattct ggtacgctga
     2341 ctcccttact aaaaaagtta gcagaaaaaa attatattaa tcgtcagcgt gaaacgggtg
     2401 atgaaagaat cgtaaatgtt acgctgactc ctcaaggtgc tgcgttgcaa aaacaagcag
     2461 aaggtattcc acaaaagatc aagtcttatc ttccacttga acaggaagaa ttggatacac
     2521 tgcaacatct gacccgaaaa atgctagata attttcatca agaccagaaa aagggctgaa
     2581 actgctagat gaacgatttt atgaggaaaa ccaactcata agataacgtc atttagcggt
     2641 ttttcttttt gctctttctc tttcttattt tcatattcca tcatataatg aataacgtgt
     2701 ttattttttg ttatttggag gttgtcgatt tgaccgttta tcagcgtttt ttggcgaatg
     2761 aaaaaatacg ccgtctcact gttttattat ttgtcgtttt tgttcttttc ttagttagaa
     2821 gtatgattac aaccattttg ctcacattta tctttactta tttgatgatt catcttgttc
     2881 aaatcattca gcgatttgtc cgaatcccta caggtgtttt agctatcatg atttatacac
     2941 tgatcgttta ccttatctat ctggcattca caaagtatat cccgatgctt attcatcaaa
     3001 catttgatat gatccagtcc gttattgatt tttacgagca tcagccaaaa gatgcagatc
     3061 ctgttttaca atatatccat acttatattg agcgaaacga tttttttgaa cagcttcaaa
     3121 atggcgcttc gattgcgctt ggctatttac aggatatagg aaaaatggca gtggcgtttg
     3181 ctatgtcatt catcttaagt ttctttttta tgattgaaaa gaaaaaaaca attgtattct
     3241 caagattgtt tttaaaaagt gaattttctt ggttctttca agatatttac tattttgccg
     3301 ataagtttgt caatacattt ggtttggtat tagaagctca atttgttatc gctcttttaa
     3361 acacgttgct cactacaatc gccttggctg tatttggctt tcatcagctg ctgagtttag
     3421 cgatcatgat tttcatttta agtctgatcc cagtagcagg tgttatcatt tcttgtattc
     3481 ctctatcttt catagcttat tcccaaggag gcatccgaga tgttgtttat atcctgatta
     3541 cgattttgat tgtccatcta attgagtctt atgtgctgaa tccgaagttg atgtccagca
     3601 aaacagaact gccgattttt tatacatttg tggtcttact tgttagcgaa cggttctttg
     3661 gcgtttgggg cttgattgtc ggcattccga tctttacctt ctttttagat gtattgaaag
     3721 tgaaaaa
//
LOCUS       DANKKG010000180         3583 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000180 DANKKG010000000
VERSION     DANKKG010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.182"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..548
                     /locus_tag="PW004_002620"
     CDS             <1..548
                     /locus_tag="PW004_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDL2727548.1"
                     /translation="LLSFAEKVYANSSIIIVSLVICLGMSYVADLVGLSSVIGAFFAG
                     IAVSQTKVKEEVYHNVEALGYAVFIPVFFVSVGLEVDFSDFSEQLLFISILTLVAILT
                     KLLGGYFGAQIAGFSQHSSLMVGAGMISRGEMALIILQIGQQSQLIEPQYYSPLVIVI
                     LLSTLISPLVLKYFTKKVYEV"
     gene            complement(657..2699)
                     /locus_tag="PW004_002621"
     CDS             complement(657..2699)
                     /locus_tag="PW004_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HDL2727549.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSYQVTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIAADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAE
                     IKEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(2757..3053)
                     /locus_tag="PW004_002622"
                     /pseudo
     CDS             complement(2757..3053)
                     /locus_tag="PW004_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
BASE COUNT          938 a          732 c          637 g         1276 t
ORIGIN      
        1 tcctactttc atttgcagaa aaagtgtacg caaattcttc tattatcatc gtatcgctag
       61 ttatttgttt aggaatgtct tacgtagcgg atcttgttgg gcttagctct gtcattggtg
      121 ctttttttgc tgggatagct gtcagtcaga caaaggttaa agaagaagta taccacaatg
      181 tagaggcttt aggatatgca gtatttatac cagtattttt tgtcagtgta ggattagaag
      241 ttgatttttc tgactttagt gaacaactac tctttatttc tatattgacg cttgtagcga
      301 ttttgaccaa attgcttgga ggttattttg gtgctcagat tgccggtttt tctcaacata
      361 gttcattgat ggtcggtgct ggtatgattt cgcgtggaga aatggctttg atcattttac
      421 aaattggaca gcaaagtcaa ttgatcgaac cacagtacta ttcaccttta gttatcgtga
      481 ttttattaag tacacttatt tctccacttg ttctcaaata ttttacaaaa aaagtttatg
      541 aagtgtgagt aaatcaaaaa gaagttgtga aagagacatc gtgaaaaccg tttatttgaa
      601 aaaaggactg tgacgataac gccacagtcc ttttactgta cttaaaaagc aattttttat
      661 tgataatttt ggttgaggta ttgcaatagt tccgatgctc gtttggttgc tgaatcatca
      721 tcttctttat tttccaacat gctgaccatc atataacctt ggttatcaag attgaaagca
      781 aacaagaaac tgttctcttg cccctttaca tcctgttttt ctttgatttc cgctgtacca
      841 gttttcgctg ccaacggaat ccctaaagca gaaagagaat gtgctgtacc atttacatct
      901 tgcacaactt ctctcagatc tggcacgatc gtttgtactg ctgtttcgct gatgacattc
      961 ttcttatctt ttgtctcttt atctgcagca atcaatttag gatagacaag tgtgccattg
     1021 ttggtaaaaa cagaatacat tgctgcttgc tggataggag taattagaag ttcgccctgt
     1081 ccatatccag tatcagctag caagatatct gagttaaagc tatcttcatt agaaatttgt
     1141 gctggattca tactaattgg caaatcaagg tcttcaccaa aaatgaattt atctaagcct
     1201 gtacgaaatt ttttctcacc cattttcaac gtttcttgtg ccgcatatat attatcggaa
     1261 tagatcaaag cagtttttaa gtctacttgt gatacatcac tactgacacg cgttacttga
     1321 taagatcccc aagaactgtc tttttgccat ttaagcccgt tgatcgtcaa cacttcattt
     1381 ggatcgatag tgccgttgtc gagaccgatt gctgctgtaa tcattttaaa cgtagatcca
     1441 ggagcataac ctgtcgcaaa tcggctgatg aatggatgtt caggattttc ttcataagct
     1501 ttgtaatctt cttgtgagat cccgtttgtc attttgtttg gatcatagct tggagagcta
     1561 gcaagcgcaa gaagatcacc ggtttttggc gttgtcgcaa cagttgatcc agcttttcct
     1621 cctagactgt caaaagctgt tttttgtgcc ttcgcatcga ttgtcaattt gatatctttg
     1681 ccattttgaa cttcatgttc gatcagaact tttttctcta caccgtctgc atctgtaatg
     1741 cttaatttcc cacctgttgt cccacgaaga tccttatcaa aagccatctc caaacctgag
     1801 cgaccgattt ttccattact gcttaattct ggattcttat caatatcttc tgctgtgata
     1861 tctcccacgt aaccaatcag ctgagcagct gcttcaccca aaggataata tctgccgtct
     1921 acttcttgaa tggtagctcc agctggaagt tctggcgttg ctccatcaat gattttcaat
     1981 gggacaaagt aatcgggttg tacccagctt tgtgaaatcg cctgattgat agcatcttct
     2041 gttaagtcga aggaggaagc aatcgctttg atattggctg ttttttcgcc tccatctcca
     2101 agtttacttg gaacgactcc taattgtttt agtttgcctg ttgttgctaa tggttcacca
     2161 tttcgatcta taatattccc tcttgctgct tcttgtgtgg tcaggcttac tttgtcattt
     2221 ccttccattt ctggaaaaac caagttgggt tgccagttga tcgtggtttg accatcattt
     2281 ctgtctaatg ttcctttata agaaagatct ttcaattcac ctaatgaggt attcatcttt
     2341 gctttatatg aaaaactgta agtagaatcg tcttttttat ctacttttag attcgatatc
     2401 tgaagtcctt tgacatcagc agcaccgtat atattctggt atttatctaa gatctctttt
     2461 tcagataatg cacttttatt tgccgccttt tttgatgtca tttctgcagc tttgttataa
     2521 tctcccttgt tcaaagcttg gacaaattgc tcaactgtct tttctccagc ttctactgct
     2581 tgggtttctt gatagtgctg gtagtaaaaa taaccgccac ccgcagctgc tattaagatc
     2641 actgcgccgg caatataagc gcctgttcga tttttgccgt gcttgtcact tcttttcatt
     2701 ttttcctcct tctcttgctt gtcattcctt tatttcacta tttatacctg tttattctaa
     2761 catatgtctt tgaggaaatc cataccacaa tccagaagca cactttctgg aaggttcgtt
     2821 tggatactac caagtaattt cccaaccgct ttgggtagct tgaatattga agttacgtct
     2881 ttcagctgct gagagatagc tttgatcact tgctgctgcc ttctgatcct cccgaagtct
     2941 ccttctttgt cttcccgaaa tcgagcatac tggagtaaag tattaccatc catcacttgc
     3001 tctcctttgt ttatatatac gccatctaaa tctagagatt tttctgcatc gatcatggac
     3061 aaagtcaaat aaggacccaa aatccaccaa tcgatctttt ttcaccgcat ctcctcctca
     3121 cacagcaatc cacttgtcag gtgaggagcg agtttgtctc atcaaataag tcagtcacaa
     3181 aaatataaag aaaaggtcag aaaccttttc gaacaagaat tctgacctag catcatctca
     3241 tcgtccgagt tttagcacta tacaacgtaa agaaaacatt cttagctatt ggaaaaagcc
     3301 ttaatccaac agtttctgtt ttacccattg gcgactttcg acgtttctgg gcaacagcct
     3361 atgtttgtat aggagcctca cctaacaagg ttattactat attctattta cgagttcatg
     3421 atagactttt cctaatgatt aggcaatgga atttgaaatg aaatagtgaa atttaacaat
     3481 ctttaatatt ttttagaaaa aaataacaaa taagttgaaa gaatcccgtt gcaaattctt
     3541 tcagttgcac caggattctt tgataatttt ttagttttat gtg
//
LOCUS       DANKKG010000181         3566 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000181 DANKKG010000000
VERSION     DANKKG010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3566
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.183"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            11..478
                     /locus_tag="PW004_002623"
     CDS             11..478
                     /locus_tag="PW004_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727550.1"
                     /translation="MIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLFAWIVSVI
                     MIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVLRIAFYYT
                     LKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTMDL"
     gene            683..2347
                     /gene="alsS"
                     /locus_tag="PW004_002624"
     CDS             683..2347
                     /gene="alsS"
                     /locus_tag="PW004_002624"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HDL2727551.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            2360..3070
                     /gene="budA"
                     /locus_tag="PW004_002625"
     CDS             2360..3070
                     /gene="budA"
                     /locus_tag="PW004_002625"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HDL2727552.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            3200..>3566
                     /locus_tag="PW004_002626"
     CDS             3200..>3566
                     /locus_tag="PW004_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HDL2727553.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNL"
BASE COUNT         1161 a          579 c          779 g         1047 t
ORIGIN      
        1 ttgcatcgtg atgataggta tgacagaaga attagcaaaa gaaaaaagtt tttcaccagt
       61 acgtttgttc ataaagacgt ttcgccaatt ttggctaaag ggattttttt attggctatt
      121 tgcttggata gttagtgtga taatgatttt tgattgtttc ttttttatac gtttttctta
      181 tggaaaatgg ttgattccgt tgtttgtttt acttgcttgt ttatcagttt cctttagcat
      241 caactactgg tattttcagg tgagaaatcc agcgtcgaaa ccaaatcaag ttctacggat
      301 agcgttctac tatacgttga aaaaatggta tgtgagttta ctagattttt tactattgac
      361 aagtttgttt ctcttctttt tcgtaaagcc ccaatggtgt attctactag gtccttctat
      421 cgtatttggt ttgatttact tcaacaatcg taaattgatg agaactatgg atctgtagac
      481 tttataaatt gaaactttca attaagaatg acagttttta tgtaaaaact gtcattcttt
      541 ttcatttttt gaaacatatg gctaaacaaa tgaaataaaa cgtgctatac ttttgaatga
      601 aacaggttta gaaactgtat caattatttg tgattatttc taactaataa ttaaaaataa
      661 agcataaaaa ggacgatgtt caatggacga aaaaatgaaa caaggttcga aatctgtagt
      721 agaaagtttg attaatcatc atgttgatta tgtattcggt ataccaggtg caaaaatcga
      781 tggtgtattt aatgaattag aggatcaagg tcctgaatta atcgtgactc gtcacgaaca
      841 aaatgctgca tttatggcac aagcaatcgg acgtattaca ggagagccag gagtagttat
      901 cgcaacaagt ggtcctggtg ctagtaactt agctacaggt ttggtaacag cgacagcaga
      961 aggtgaccca gttttagcaa ttgccggaca agtaaaacga agcgatctat tgaagttgac
     1021 ccatcaaagc atggataatg cagcactgtt ccagccaatc acaaaataca gtgcagaaat
     1081 ccaagatcca gaaacaattt cagaagtgat tgccaatgct tatcgtatgg ctaagagttc
     1141 aaaaaaaggc gcgagcttta tcagtatccc gcaagatgta gtggatgcac ctgtccaagg
     1201 gaatgtcatc aagccattga gcgatcctaa attaggttct gcttctgcag atgatatccg
     1261 ttacctagct gaacgcatta gagaagcaaa actaccggta ctattagtag gtatgcgagg
     1321 atcaagtgaa aaagaaacgt tagccatcag gcaattagtt ggaaagacag cattgcctgt
     1381 tgtagagaca ttccaagcag ctggagtgat ctcacgtgaa ttagaagcgc atttctttgg
     1441 tcgtgtgggt ttatttagaa accaaccagg agatatgctt ttgaaacgaa gtgatctagt
     1501 tattgctatt ggctatgatc caatcgaata tgaagcaaga aactggaatg cggaaaaaga
     1561 tgcgcggatc attgtgatcg atgaagcgcc agctgaaatc gatcctttta tgcagcctga
     1621 aagagaattg atcggtgata tctcagccac tcttgattta ttgacaggtt cattagagcc
     1681 tcaacaagtg tcagaagatg caaaagaata tttagcatct cttcaagcag aattaacgga
     1741 gcgggacatt gttcaatcaa aaggagaagc aggtatcttg catcctttag aagttattaa
     1801 cacgcttcag tcaaaagtaa cagatgatat gactgttacc gtagatgtag gtagtcatta
     1861 tatttggatg gcacgccact ttagaagtta tgaacctcgt catttgttat tcagtaatgg
     1921 tatgcaaacg cttggtgttg cattgccatg ggcgatttct gctgcattgg tgcgtccaaa
     1981 tacacaaatc gtatcagttt ccggcgatgg gggattttta ttctctgccc aggatttaga
     2041 aacggcggta cgtaaaaaat tgaatatcgt tcatttgatt tggaatgatg gacactacaa
     2101 tatggtagag ttccaagaaa aaatgaaata tcagcgggcg tcaggtgtag actttggtcc
     2161 agttgatttt gtgaaatatg cagaagcttt tggcgctaaa ggaatccgag ctacaagtgt
     2221 agaggaatta gaaaaagctt tagaagaagg atttgcgaca gaaggacccg taattattga
     2281 tattccgatt gattaccgtg ataatgagaa attgggagaa acaatcttac cagatcaatt
     2341 ctattaagga agtgtcgaca tgactgaaaa aatcttatac cagcacggga cattaggagc
     2401 tttgatggct ggcttgatgg atggaacaga aacgattgct catatattag aaaaaggaac
     2461 tctgggattg ggaacattgc atgggcttga tggtgaagtc atctttttag atggcgtagc
     2521 ttatcaagga agatcagatg gctcagttgc ccaattagat ggaagtgaac ttacccccta
     2581 tgcagcaatt acagatttta cgccagatac tagtttttct gtctcaaaaa ctgcagatgg
     2641 agaacaattg aaaaaagaca tattagtaag agaagccggg gaaaacctat ttttggcagt
     2701 gaagataact ggattattca aaaatatgca catccgtatc atgccgaagc aagaaaaacc
     2761 ttacagaaga ctagcaaaaa tttcagagtc tcaaccagaa ttccaacaaa gcaatgtcca
     2821 aggaacatta gtcgggttct ttacgcctga attattccaa ggagttgctg cagcgggatt
     2881 tcacttgcat tttatcgatg atacacatac atttggcgga catgtaatgg attttgaagt
     2941 ggctgaagga aaagttgaga ttagccgcat ctcgtccctt gtacagcatt ttccagttga
     3001 tgatccgact tttgttcacg aaacgattga ttatgctgat ttagcagcag aaatccaaca
     3061 agcagaatag aaaaagacag cgagatgcgt tcaaggtgat taacctgacg tatctcgttt
     3121 ttttgaggag gaaacctagc gtttacaaaa aaactgagct ataatcttct ccatagatca
     3181 aaaaggagcg gtttttatca tgtggacaga aaaacagaaa aaagatgcat acgaaatttt
     3241 attattacag caacaaggat tattagaagc agaagataac tggttagcaa atttagcaaa
     3301 ttcatctgca ttgttgaatg agactttgcc tgagactgtc tttgcaggat attatttatt
     3361 cgatggtaat gaattgattt taggaccgtt ccaagggcga gtttcttgca cacgtatcaa
     3421 gatgggaaaa ggagtttgtg gagaatcagc tgaaaaacaa gaaacattga tcgttgatga
     3481 tgtcaaaacc catgcaaatt acatttcctg cgattcagcc gcacaatcag aaatcgtagt
     3541 gccaatggta aaagaaggta atttaa
//
LOCUS       DANKKG010000182         3523 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000182 DANKKG010000000
VERSION     DANKKG010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.184"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            238..1938
                     /locus_tag="PW004_002627"
     CDS             238..1938
                     /locus_tag="PW004_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDL2727554.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            1908..2126
                     /locus_tag="PW004_002628"
     CDS             1908..2126
                     /locus_tag="PW004_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HDL2727555.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            2146..3036
                     /locus_tag="PW004_002629"
     CDS             2146..3036
                     /locus_tag="PW004_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HDL2727556.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            3054..3338
                     /locus_tag="PW004_002630"
     CDS             3054..3338
                     /locus_tag="PW004_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727557.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
BASE COUNT         1241 a          576 c          731 g          975 t
ORIGIN      
        1 tcttttattt tacaacattt gcattaaaaa agcttgtttc ttttatttta aaattccatt
       61 acaaaatcac gttttcattg aaaacactct cattttcaat taatttttgt gatttttgta
      121 tttttttatt taaaggaggt tttatgaatt gttttcttat ggaatctaca aagtcgaata
      181 cgagactcat gaccttttta tgacgtactg gtatacttga agaaaaagga gagcgcgatg
      241 gagataaaaa aagtttactt ttcacagaaa atgtcgctgt attcgcctat ccagcaagca
      301 aaacaacccc ttccattttt tcaaagtcat caagaaagta gagacgcttt ttggcgttct
      361 gcaaaaaagc aatgtgcttt taaagcagta tcgaaaaaat ataccgtcta ttttactatt
      421 gctaaaagta gaggagatgt tatttttaat aatctgttga ttgaaggtga aacttttgaa
      481 tggaaaaaat tttttcggta tatggaagct tgcgctcgtt tttttattaa agaaaactgt
      541 tgttttttat ttcaatctcc tctatcagaa gaatggttgc gtatttttca taaaaacggc
      601 tatcaaggaa ccacacaatt aaataaaaag ctggtctatc atacagcact tgttttaggt
      661 ggaggaggag cgcacggtgc ttatcagatt ggtgtgtggc aggcgctgaa ggaacatgat
      721 atcaactttg agattatcac aggtacctcg gtaggtgcgt tgaacggcgc attgatttta
      781 caaggagata tgaaaaaagc tgtgaattta tggaaaaagc tgtcgacaag acaagtgctt
      841 gcgttaccag aaatggctgc agttgaagac cttcgtgaac gcttctatcg agaaagacgc
      901 caaatgacga aaacagcact aattgaaggc ggggtttctt ctgctccatt ggaaaagatg
      961 gtcaaagaca atttggattt gtccttactc aaacataatc ccaaaatacg tttatatacg
     1021 gtgtctacta aactgccaga actagcggaa gtagtgactg atatccaaca gacaaaaaca
     1081 gaagaaatac cagattggat actcgcctct gcttcatttt atcccgctat ggcttatcga
     1141 aagatcggaa aaaataaata tgcagacggt gggtatcgca acaaaatacc tattgatatt
     1201 gcgattaaca aaggagcaac agaggcattt gtcgtagatg tgcaagggcc aggcccagcg
     1261 aaaaaaattc gtatgccaga catttttatc cattggaaat gtcaaacatt gtggacacta
     1321 ggcagctttc tgctttttga tagccaaagg aaccaattaa atttacaatt aggctatctg
     1381 gaaatgaaaa aacgattagg ctattactat gggaactggt atacatttca ttctgttaaa
     1441 cctgcaagaa catactggcg aggatttttg tcttacttaa caaaagaaat tcagttagaa
     1501 ttatcttttt tcaaaagcat aaaattttgg caaaaactgc gtaatttata taagaacagg
     1561 gtcgtaccag agacatgcgg attggcgatg ctagaacttt tagcaaaaaa acatttttta
     1621 ctgcccaatg aaatctatca agtagacatg atgattcaaa tgatctgcca acaagatata
     1681 aaaagtatgc cggatgattt attggcacaa attggtcaat taagtactga agaatggcga
     1741 aggtatcgaa aataccagca aaaaatacaa aatgagcgca cgaaaactgt ttattttgat
     1801 tatctgctac aaggaaaacg caaggatcgt ttgcagcatg aattgcttaa gcaaccagtt
     1861 gacacattac tgatcctata tttatattat ctcaaggagg aacaaccatg gcacaagaat
     1921 tttcatatga aatcgtagaa gaaatcgcta tattgtcaga aaacaacaag gggtggcgta
     1981 aagaactgaa cctagtcagc tggaatggtc gcccgccaaa attcgatctt cgagattggg
     2041 cgccagacca tgaaaaaatg gggaaaggcc tgacattgac gaacgaagag tttgaacaac
     2101 tgcaaaaagc aatagaaaat atgtaatgag gaaggaccct gtcgaatgaa aagtatgact
     2161 ggttttggca aagccatccg cgaaacaaaa gcgtatcagt tagatattga aataaaaagt
     2221 gtcaaccagc gttttctaga tgttcaaata cgaagtccta aaatcctgaa ctttgttgaa
     2281 aacgattttc gtcagcttat taaacagcag ttaagccgcg gtcgaatcga agtatttgtg
     2341 aatctagctt atattggcaa aaacgaaaaa cagatcgtca tcaactggga attgattgat
     2401 tcacttatca ctcaattgac agacggaatt caacaacgat acggtagtca aaatcaagtg
     2461 tcaattagtc ggatggcgga acgcttcgcg ctggacgaaa acttcgtaac ggtagaagaa
     2521 aaaccggaag agagcatgga agaattggca cagttagctc tagatacatt gaaaagcgca
     2581 cttgattcta ttgaggcgag tcgagaaaaa gaaggtcaag ctttagcttt agtgttgaaa
     2641 aagaatactg aagaattcaa agaagtacta aaaaaactcg atagttttgt agaaatctat
     2701 gaaaaggaat ttcaaataag gtatcaaaaa aagctggagg aatttcttgg agcgaaagtc
     2761 gatgagcaac ggttgttgac ggagctagcc atcttgttgg aacgcggaga tattcacgag
     2821 gaactggatc gtctggcgat ccatgtacaa aaactagatg aacttatcga agcacaatgt
     2881 cctgtaggtc gggaattaga tttcctgatc caagaaatga atcgggaaat caacacgatc
     2941 ggttccaaat caaatgcaat cgaaattaaa aatcaagtcg tccagtctaa gacgatctta
     3001 gaaaaaatca gagaacaagt acaaaatatt gaatgacgaa agaggtagag ataatgaaat
     3061 attatgacgt aacgttccac gaattaagtg gaaaaactgt ggttaaacgg gatatccctt
     3121 ctgaaaaaaa cggctttgac gtatggaaag atgcctgtgc agactataat gagaacgaac
     3181 tgtttatttt gatcaacgac ggtgcttacg tgacgatgaa tcggaaattt attgttcgaa
     3241 ttgacacaga agaggtagaa gatccgacag aaaaagcacg ttctcgaaaa gatgagatca
     3301 tgggtgtagt gaatactttg tccaatatgg gcttttagca aggatgctga acaaagaaat
     3361 acaataaata atcagaattg gatttcttcg cttagcttct ttgctctagg aatacattta
     3421 aaggttgtga caaaattcgt gtcacaatct ttttttattt tgaataagtg atgttcaaaa
     3481 accattttct cggtattaag taagcgcccc atagaacagt acc
//
LOCUS       DANKKG010000183         3513 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000183 DANKKG010000000
VERSION     DANKKG010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.185"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..331
                     /locus_tag="PW004_002631"
     CDS             <1..331
                     /locus_tag="PW004_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727558.1"
                     /translation="NNKKMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKI
                     VVLSAGGQLMGDGKSELVSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKE
                     TNSVELE"
     gene            345..1034
                     /locus_tag="PW004_002632"
     CDS             345..1034
                     /locus_tag="PW004_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727559.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1159..3510
                     /locus_tag="PW004_002633"
     CDS             1159..3510
                     /locus_tag="PW004_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HDL2727560.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
BASE COUNT         1189 a          460 c          742 g         1122 t
ORIGIN      
        1 taataataaa aaaatgagtt tgaccaaaaa tatctattct atgtcagatg aagaattgta
       61 taaagaattt ggtgatttat ccggggaaca gttagaagaa agtattccag gaattagtga
      121 ctcagataaa attgttgtct tgtctgctgg tgggcaatta atgggagatg gcaaatccga
      181 attagttagt tataaacaaa atcacagaca gtttattgat gttaatactg attccaacgc
      241 tatttcgatt aaggatctaa aagagctttt aaatagtaaa agaaatgaat taaagcagat
      301 aaaagaaact aattcagtgg aattagagta aaggaagatt cattatgaaa aagatttttg
      361 gtgtttctgt tgggctactt atgatgttaa catttggaaa tgcagtatat gctgctgaga
      421 aagaagttat ttcagatcca gagcagtacg cctcacgata ctttgctgat tttgaagaag
      481 aagataaagt acttgcattg ccagaaggtg gttttttaaa aggggaagct aaaattgtat
      541 ctatggataa tccagatata attcaaggtt catataattc agaaactgat cctgcttcag
      601 tttcagtgac agaagcaaaa aaaattgttg ctgaaaaatt caaaaatgat attgaaacta
      661 ctccaattat gcctcgagga gcatttccac ccgatgttgg tactagcgga caccttcttg
      721 tactagcagg aggaggaagc tataaatcac agccatttag tggtgcggga tggcgttttg
      781 gaggtatggg tgtaaaacca gctgatggta caggcaactt tttacgttgg aaatcctaca
      841 ttgacgatgg aagagtagga aatgtaaatc aagccaacgc aactaaatct ggttctattc
      901 aaggaatggc attgtatgct cgtgaaggat tcaaatactt tgcaccaaac gggcctggtt
      961 ggacaatgaa tataggtatt tattacactt ataatcctgt tgcaggtacg tattatgttg
     1021 ttcaaaatat ttaataaaac agcacaaaga gatttctttg tgctgtttta ttatttttag
     1081 ttgttgacaa acactaaaat ataaaatata atcaacacat aaacgaacgt atgttcggca
     1141 agaaagcagg ggatttttat ggatgagatg gttttaagta ctcagaaatg gctaaacaag
     1201 aaatacagta atgttacagg gtttgataag gttccagaaa atgggcgaac agggtggcct
     1261 acaatctatg gacttattga aggactacaa gtagaacttg gtattactaa tctagtagct
     1321 aattttggac ctaccactga aaaaatgtac gataatcagg ttactcctaa atggggcaaa
     1381 aatctaccta aaaatattgt atttcttatt caaggtgcct tttggtgtaa ggggattaat
     1441 ccaggtggct tcgatggggt atatactcca tatttagata ctgctgtaaa agagttgcag
     1501 actgatgcag ggtttaatgg aagcgctgta actggagttt tggatgcgaa atgggccaaa
     1561 gcgttatttg atatgagtgc ttttgttctt gttcccggcg gagatagtaa aattagaaaa
     1621 atgcagcaat ggttaaatgt aaattattta gaatacacgg gaataatgcc ttgtgatggt
     1681 atttatcaaa gaaattcaaa tcaagcatta atttttgcat tacaagctga actaggatat
     1741 tctcccagtg aagcaacagg gtcttatggt aatgggacaa ctagtaaaac ttatcctgtg
     1801 tctgaaggta actcgggaaa ctatgtaaga ataattcaat atggattata tgttaatggt
     1861 tattttgaag acggtacatt cgatggtatt ttcactaaat atatgggttt agaagtactg
     1921 gcattccgaa aatttatggt attgcctgaa tatactgaaa tagcagatgt tgttgtaata
     1981 aaaggactat tgtctagcgc tggagatatt agaagatctg cagatggcgc agatacttct
     2041 acgcaattaa ctagatctca aatccaaaca ttggttgata atgatataaa aatagttggt
     2101 agatatctta ctggtactgt tggaattgga aaagatgaac gaaataagta tctaactaca
     2161 gaggaattga ataatatttt ttctaaaaat ttatctgtgt ttcctatcta tcaagatgga
     2221 ggtgcagcat tagcttattt tacttatgat cgtggcttat cagatggtaa gaaggctata
     2281 gatgcagcaa aaaatctaaa tataccatta acaacagtta tttattttgc tgttgatcta
     2341 gacatgttag gggaagaaat tcttgcatat gctggagaat attttaaggg ggtatctgct
     2401 gtaatgtcat atagcgggta tcaaacgggt gtttacggaa caagaaatgt ttgttctcaa
     2461 ataatcaata acgggtatgc ttcgtttgca tttgtttcag atatgtcgac tggttatagt
     2521 gggaatttag gttttcctat gcctagacaa tggtcttttg atcaatttgt tgaatttacc
     2581 ataggaagtg gcaacggagc tgttggtatc gatttaatcg ctacatcggg tagagatagc
     2641 ggatttaatg agttatcaaa tgatactaat aatgataatt acattgccaa atacaatcag
     2701 aaggttataa atcagatggt acgtgcgtat caatatctat cacaagctgg tcttgataat
     2761 ccttggaacc ctcatttgac attttataga tatgtaaatt attctggttt gtcatgggac
     2821 atcatttctt ctcctgtaac agagcatgat agaaaaattt atgatgaata taggaataaa
     2881 ttaactaata gcgagggcct gtataattat tttatagatc ctaatactgg ttcaataata
     2941 ggtttgcctc acttaattgt gacgttacaa tcacagatgt ttctagccga cacaatcaat
     3001 gatagtgttt ctgactttgc aggttggtta ggagatttaa tgacttgctg gggtgaagtt
     3061 aagaaattag gaatacaaat ggagcaaggt gtatttgcat tagtaggtac ggctgcaggt
     3121 ggaacattta gtctagaaga tttataccaa gatgttgacg cggtaaatgt ttatacctta
     3181 gcaaaaagaa attctgattc aacagcttta tcggctttaa aaacgtacta ttgtgataag
     3241 ggtgctattg atagatttaa tcaatttgtt aagaacagat ttggttcttc agataaaatc
     3301 gcttcagtaa caaatagttt attagaaaat gacgaaacag ttgatatctt aggtgtaggg
     3361 ataatttcat ttttcattga taaaatagtt aataattcac ttgggacaga ttttacttat
     3421 cttgatttta ttggagatga ggggaaagtt gcaaatggat tttctgaaaa aattattcaa
     3481 tttcaagaaa atagaagtaa acttaattaa gaa
//
LOCUS       DANKKG010000184         3441 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000184 DANKKG010000000
VERSION     DANKKG010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.186"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            395..1603
                     /locus_tag="PW004_002634"
     CDS             395..1603
                     /locus_tag="PW004_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HDL2727561.1"
                     /translation="MKKRITLALLSSVGLLLAACGQEESTSSQNSSQQAIVSTSQETS
                     ESQSSSKEENKEQTEAYYTSDTGSVTKVDAQTHEVVDTIAIDGSAHNVQISPDEKVIG
                     VTIVPSEGHAEDSSGSNESMEMEHDEDSDTSMSNESMEMGQDEDSDTSMSNESDDEKG
                     IAAFYDAETGEKLAEVEVGSHPAHIVFTNDGNYAAVSNNGENTVSVIDVSTYEVVETI
                     PTGEGPHGFRIAEDGKTAYVANMGEDTVSVLDLESMEEVNRITVGSTPATTGITSDGQ
                     TLLVTLNGENALAIVDLTTNKVEKVAVGNGPIQLFVQHDDQYVFVANEGTEENPSNTV
                     SKVDLNSNEVVETIEVGSGAHGVVISEDDKYTFVTNAFDDTVSVIENETSEVIVTVEV
                     GETPNGITLK"
     gene            complement(1773..2606)
                     /locus_tag="PW004_002635"
                     /pseudo
     CDS             complement(1773..2606)
                     /locus_tag="PW004_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007723271.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
     gene            2729..2983
                     /locus_tag="PW004_002636"
     CDS             2729..2983
                     /locus_tag="PW004_002636"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2727562.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            3094..>3441
                     /locus_tag="PW004_002637"
     CDS             3094..>3441
                     /locus_tag="PW004_002637"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDL2727563.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKPIISKENLLNQDFSTETICEKWAADITYIPT
                     KKNGWCYLSSIMDL"
BASE COUNT         1223 a          536 c          650 g         1032 t
ORIGIN      
        1 acgaagaata ccaacaaaag aagaaaatat tgaaaagtta gttgggtaag tctgaagttt
       61 catgctcgaa ctgagttttc tcaaaaatat attccggaaa tgaactattc atatcaaaaa
      121 gccaagatca tatacaaatc ttggcttttt gatatggttt ttttctacat aaaatcaaca
      181 cattcatata ttattataaa aacagaaaca taaaataaat caaagcaagg ggataaatta
      241 tgcaccacca tgaaagtaaa gaagagaaat caggaacgca gtaccacaat cttttcagaa
      301 acctacggaa aaattatgaa taattcaggc aattatagag caataaaaat agtacgaaaa
      361 gtaaaaagta cgtagtaaaa aaggggatat taaaatgaaa aaaagaatca ctttagcact
      421 attgtcgtca gtgggtctct tgcttgcagc ttgtggtcaa gaagagtcaa caagtagtca
      481 aaattcttct caacaagcaa ttgtatcaac gtcacaagaa actagtgagt ctcaatctag
      541 ttctaaagaa gaaaataagg aacaaacaga agcctattac acttccgata ctggaagtgt
      601 tacaaaagta gatgctcaaa cgcacgaagt tgtggatacg attgccatcg atggatccgc
      661 acataatgta caaatttcac ctgatgaaaa agtcattggt gtaacaattg ttcccagcga
      721 ggggcatgct gaagacagtt caggtagtaa tgaaagtatg gagatggaac acgacgaaga
      781 tagtgatacc agtatgagca atgaaagtat ggagatggga caggacgaag atagtgatac
      841 tagtatgagt aatgaaagtg acgatgaaaa aggtattgca gctttttatg atgctgaaac
      901 aggtgaaaaa cttgcagaag ttgaagtagg gagtcaccct gctcatatcg ttttcactaa
      961 cgatggcaac tatgcagcag tttccaataa tggagagaac actgtttcag tcattgatgt
     1021 atctacttat gaagtggttg aaacaatccc aacaggcgaa ggtccacacg gttttagaat
     1081 cgctgaagac ggaaaaacgg cttacgttgc caatatgggt gaagacactg taagcgtcct
     1141 tgatttagaa agtatggaag aagttaatag aataactgtt ggcagtactc cagctacaac
     1201 tggtattacc tcagatggac aaacgctctt agtaacatta aatggagaaa atgcgttagc
     1261 aatcgttgat ctgacaacca ataaagttga gaaagtagca gttggaaatg gtcctatcca
     1321 gttgtttgtt caacatgatg atcaatatgt atttgtggca aatgaaggta ccgaagagaa
     1381 tccttccaat accgtttcaa aggttgattt aaatagtaat gaagtggtcg aaacaatcga
     1441 agtgggtagc ggtgcacatg gagttgtcat tagtgaagac gataaataca cctttgtcac
     1501 gaatgcattc gatgatacgg tcagtgttat tgagaatgag actagtgaag ttattgtcac
     1561 ggttgaggtt ggtgaaacac caaatgggat tactctgaaa tgattattag tgttaagtta
     1621 aactaaaaaa tcaacttaat gatttctgaa aagggataaa tattctaacg tttaaaaatc
     1681 ttaattagtc ctaaattaaa tggaaaactt tatcatgaag ctgaaaaagc ggttcacttt
     1741 acataaaagt gaaccgcttt ttttgatgat tactatcttt tgacaggaag aataacggta
     1801 aatgtagaac cttttccata atcacttttg accgttatgg ttccgttatg agcctttatg
     1861 atacttttta cgatagataa gccaattcct tgcccgccta gaaaactatt tcttgaaaga
     1921 tcagacatat aaaacctctc aaaaatacga ttaatttctt ttgggtgaat tccttctccc
     1981 gtatctgcga tagtcaataa agcttgattt ttttcgttgc gaatttttag agttattgtt
     2041 ccttctgggg gtgtaaattt aacagcattg gataaaagat ttgtaatgac ttgacttatt
     2101 ttgtccctat cagcggtaat gaatagagaa tccccattta atgcataata aatatttttc
     2161 ttagcaaaaa gagcttcaaa attagaagat atattttccg cgagagcata taaatcgaaa
     2221 gatattttgt ttaaactatc ctgatgactt tcaatttcat taattttatc aatgcttcct
     2281 ataaggcggg taatacggtt cacctcgtca taacaacttt gaattcggtc atctattatt
     2341 tcccatattc catctatcat agcttccaga cttcctttta gtgttgttaa tggagttctg
     2401 atctcatgag cgatatcaga agataattgg tttctgatat cttgttgatt ctcaagttga
     2461 acagaaagag catttacaga tgaaatgagt tcatctagtt ccttgatatc agttttttca
     2521 ggagataaac ttgtgtaatc tcctttagca atttctcttg taaaatcctt tactctcact
     2581 ataggtccac ttatcttttt agcaacccgg agtacatctt catgtcgatg cacaaaaaaa
     2641 attgttaact ggtgttagta tcaaatctta gacagctttt cgtagagaca agaataaatt
     2701 aaacttatct acgagaggat gaatttttat gacccgatat gaagaaaatt ttaaacaaat
     2761 gattgttgaa ctgaatcaaa ctggacgttc tgttcgaggg ttagcgaaag aatatggctt
     2821 atctgaagca acgatttaca aatggaagaa tttatattta cctgatcagt ccacaggact
     2881 gactggaaaa gaagtagctg aactgagaaa agaaaatgct cgtttaaatg aggaacttga
     2941 aatcttaaaa aaagccgcag ccatattctc tcggaaaacc taaattcgct tgttcaattt
     3001 attgaaaaat ggtgtaagga ttacactgtt tctttgttat gtcggttatt agaaatccct
     3061 agaagtgtct attattttta taagaataaa ccgttgacag ctacagaaat cagaaataat
     3121 aagttgaaaa agaagatttc aacaattttt tttacgaata aacaacgcta tggtgccaca
     3181 aagatccatc aagttttatt aaaagaaggg atttcagtat ctcttaaaca tgtccaaaag
     3241 ctaatgaaac aattaaattt aaggtcgatt gtagttaaaa aatatagacc tcaaaggtct
     3301 aataaaccga tcatttcaaa agagaatctc ttaaatcagg acttttctac tgaaacgatc
     3361 tgtgagaaat gggcagctga cattacgtac attcctacta agaaaaatgg ttggtgttac
     3421 ttatcttcaa tcatggattt a
//
LOCUS       DANKKG010000185         3431 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000185 DANKKG010000000
VERSION     DANKKG010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3431
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.187"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(12..161)
                     /locus_tag="PW004_002638"
     CDS             complement(12..161)
                     /locus_tag="PW004_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727564.1"
                     /translation="MKKKVIPYCPKPRNAFERGLFKYFEGKKGQDHKEENQKKKSKKR
                     SEYIF"
     gene            1129..1455
                     /locus_tag="PW004_002639"
     CDS             1129..1455
                     /locus_tag="PW004_002639"
                     /inference="COORDINATES: protein motif:HMM:NF013698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2727565.1"
                     /translation="MFKIFRKNCCGLDVHKTWIYACIGITDANGRTVYKQARFSSFTK
                     GLNELAEWLAKYNCKDVCLESSGKFWIPVFNILEKTCFVTLAHPKYTKPQKGNKTDRK
                     DAKMDL"
     gene            1436..2371
                     /locus_tag="PW004_002640"
     CDS             1436..2371
                     /locus_tag="PW004_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HDL2727566.1"
                     /translation="MQKWICDLFMCDMIKPSFIPGLEIRQLRDLMRYRTKLTNMKTSE
                     KNRALNCLTVSNLKLDDVFSDVFGKSSTSIIQYLLEHPGESFDVRPFIHARCKTPVEE
                     IQAAVEGAISVPQAIKLRQCLNHINEIEKHREEINLEIQALAEPFSPVLELLRTIPGL
                     DTQPITAIAILSEIGPDMSVFPTSKHFISWAGCCPRNDRSARRTKSTRISRAGQSLKP
                     LLVQIANALLRSKEHPEFKNRYRRIKANRGHKKAIIAICKMLLTAIWNVLSKVEPYSA
                     KGYLEPKPAKEEKVLTQSQALDLLRKRGYTIIDSA"
     gene            3000..3251
                     /locus_tag="PW004_002641"
     CDS             3000..3251
                     /locus_tag="PW004_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HDL2727567.1"
                     /translation="MKSNQKSGFYTFIKAISSVLECDCIIGPYSMLIKMGFPSTESLD
                     SFINDLSKFGRTNTQIVFSTLVSLMVYQFWNVYHALLLK"
BASE COUNT         1071 a          683 c          591 g         1086 t
ORIGIN      
        1 ttttattctt tctaaaaaat atactcactt cttttcttag acttcttttt ttgattctcc
       61 tctttatggt cttgtccttt tttgccttca aaatatttaa ataaacccct ttcaaaagca
      121 tttctaggtt ttggacaata aggaatgact tttttcttca tataaatacc tcatttaact
      181 atttgatatc atcacattat caaaaaaaat aggtttttta ttaaaactat cttgaccttt
      241 cgggagattt cactgtgatt tatttttatt cccatacttt tttcgttggt cactttcgta
      301 tacctaatct aagcgatcat cggcgacggt gcttttacaa cgaatggcag gacaaaacaa
      361 ggggcggaaa aacttttttg cctatttcat aaaaaacttt ttcctacagc aatcccttgt
      421 tttgtcctac cacacgttgt gaacccaaag cacgccgacg aacgcttagc tatacgaaaa
      481 tgacctccaa aaacaaaaaa gtgaggtgaa tagaaacaaa aagaaattga aatctcccaa
      541 aatgtcgtcc gcctcggaga caacttgctg ctgaaaatgg ttggttaatt catttaagta
      601 ttaaataaga taaatcatca aattaatcgg aaaaatagca tgattggcct tatcaaaaat
      661 aaactgtaaa aacttatatt tctatgagtg gtggaacaat gctaatacca aattaattta
      721 tcgattaccc acctgcacaa accaaagata gcattattag gaaacgaatc aatcagcgaa
      781 tccagcatta taatgaggat actaaaaata aagttttcat aattataaat ccgccttcct
      841 acgaaaggca ccaatgaggt gatgaaaacc ttagagcgct ttctttcttt ttttattccg
      901 tgttaccctt tccttgcagg tccctggtac actacagaac ccgaggaggt gagtggcggg
      961 gctgtatagc ggcaaccccg cataattata tggtgtcggt catccgttaa ggcatcaatg
     1021 aatggcgcaa acccgtagcc cgtaaactta tctcttccga agttgactcg atttcgccgg
     1081 atgaccagtc cctaccaaac ctgtactatt tttttacagg aggttgctat gttcaaaatc
     1141 tttcgtaaaa attgttgtgg cttggatgtc cacaaaacgt ggatctatgc gtgtatcgga
     1201 atcacagatg cgaatggtcg tacagtgtat aagcaagctc gcttctcttc ctttacaaaa
     1261 ggtctcaatg aattagctga atggcttgct aaatacaact gtaaggatgt gtgtttggaa
     1321 tcttccggta aattttggat tcctgttttt aatattcttg aaaaaacttg ttttgtcaca
     1381 ctggctcatc cgaagtacac caaaccccag aaaggaaata aaacagatcg caaagatgca
     1441 aaaatggatt tgtgatctct ttatgtgcga catgatcaaa cccagtttta ttcctggtct
     1501 tgaaatccgc cagcttcgtg atctaatgcg ttatcggacg aaactgacca acatgaaaac
     1561 aagtgaaaag aatcgtgctt taaactgctt aaccgtttct aatctgaaac tagatgatgt
     1621 ttttagtgat gtgttcggaa aatcatccac ttctatcatc caatacttgt tagaacatcc
     1681 cggtgaatca tttgatgttc gaccatttat ccatgcccgt tgtaaaactc ccgttgaaga
     1741 aatccaagcg gcagttgagg gggcgatttc tgtacctcaa gcgattaagc ttcgccaatg
     1801 cctcaaccat attaacgaga ttgagaagca tcgagaagag atcaatctag agattcaagc
     1861 tcttgcagaa cccttctccc ctgttttgga actacttcgt accataccgg gcttagacac
     1921 tcagccaata acagcgattg cgattctttc agaaatagga ccggatatgt ccgtgtttcc
     1981 aacttcgaaa cactttattt cttgggctgg atgctgtcca agaaacgatc gcagtgcccg
     2041 acgaacaaaa tcaacacgta tctctcgtgc agggcagtcg ttgaaaccgc tacttgtaca
     2101 gattgccaat gctcttctca gatccaaaga acatccagaa tttaaaaatc gctatcgtag
     2161 aatcaaagcg aatcgtggac acaagaaagc tatcattgct atttgtaaga tgctcctgac
     2221 cgctatctgg aatgtcttat caaaggtgga accctattct gctaaaggat atctggagcc
     2281 aaaacctgcg aaagaagaga aagttctgac gcaatcccaa gctctggacc ttttaagaaa
     2341 acgcggatat accattatag atagtgctta aaccaaagtt acgttgattc ttcctaaata
     2401 gactcgttgt cttccatttc aaaaattttg aaaacggtag gcttatttag tgtaccgttc
     2461 ttcaggtatc ttcaaataga tttattttca aacttatttc ctttcttctt ttactctcct
     2521 atagcttagc atttaagtgg gcactcgtgt caccttgcta gatagctatg aatgccgtaa
     2581 taagcctcct agtatatagg cagactgcca attataactg attcaatcca cattatgata
     2641 aaatccacct tttcgtttca tatgtgttga aatatcacat atgtcaaaag tgcacgagtc
     2701 ttttcattca gtgctttgta aatactcaaa attattcctt ttcactttaa aaaaaagata
     2761 attagtacaa aatcagaaat gaggaaagta ttaggatgga ctagaaagga gttgttgaat
     2821 ttgttgcaac agattggtca tttatcaatt aaaaattagg tgaagtttta ttcatcactg
     2881 cagctgctgt ttcccagaga ataaaacgtc tagaagaaac tggtcagtta ctgtattatc
     2941 aagcagcaat tgactacgaa caagcttgat tactaaccaa agcgtttatc cacctgttga
     3001 ttaaatcaaa tcaaaaatca ggtttttata cgttcataaa agcaatctcc agtgtactcg
     3061 aatgtgactg tatcataggt ccttattcga tgttaatcaa aatggggttt ccttctaccg
     3121 aatcattgga cagttttatc aatgatttat caaagtttgg acgaaccaac acccaaatcg
     3181 ttttctcgac tcttgtttcc ttaatggttt atcagttctg gaatgtttat catgcgcttt
     3241 tattaaaata gaaaagagct tttttcatgc gacaaagcag tgatcattta ttggcacatg
     3301 caatctttgt ggatccattt tttgaaaata cgatatttag ttttgaccca aaaatcttta
     3361 aatacgtatt tgataattta tcttaacaac cagataattt tattcagcac cgtattatct
     3421 atattgaatt c
//
LOCUS       DANKKG010000186         3399 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000186 DANKKG010000000
VERSION     DANKKG010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.188"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..350
                     /locus_tag="PW004_002642"
     CDS             <1..350
                     /locus_tag="PW004_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727568.1"
                     /translation="YYDLLEVFASAKILDKKKREEVNKILESENSGYRLSESNEFIPI
                     TDDISIKSINAVSESPFNYAKNHIHKSLTYISEKENQDYNTVVREAISAVESCLIEFS
                     GLPANKKILWELL"
     gene            705..941
                     /locus_tag="PW004_002643"
     CDS             705..941
                     /locus_tag="PW004_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HDL2727569.1"
                     /translation="MNIKKRKIREVGNSIVVTLSKESLLQKGLKPGDTIFIDEDKMME
                     AIVKEESKIDLEIDMYVNQALSEYDAAFKELVEK"
     gene            938..1351
                     /locus_tag="PW004_002644"
     CDS             938..1351
                     /locus_tag="PW004_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HDL2727570.1"
                     /translation="MIYLSEKDIVKLNVIQIKKYSPRETIGVIDKNALSMAVNQPKQH
                     IFGKDLYPDKFSKAAVLLINLIKKHPFHNANKRTAFLAAYVFLKLNGYSLKMKNQEVV
                     EFVVEIATYQGDFDDLKEATTDILKKNSTKTDSKK"
     gene            1870..2820
                     /locus_tag="PW004_002645"
     CDS             1870..2820
                     /locus_tag="PW004_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HDL2727571.1"
                     /translation="MERNSENKMTGRNPKVFISYSWENKENKEWARELADKLLENGID
                     AYIDQYDLSLGDRLPQFMEQQISDSDYVLIICTPEYKKKANNRTSGVGYEGHIISAEL
                     LSGKNERKFIPIIKEGRASEVLPTFLAGKLGIDLSDSQHYDENLDDLITTIFGVKRKP
                     ALGDIPRHIKGSPLLPIEHNENKDIEILGIITDKVTVPKMDGSRGSALYKIPFRLNRV
                     PSQLWKELFVRNWNSPPRYTTMHRPGIASVVDDEILLNGTTIEEVREYHRETLKLCIE
                     KSNTQEKKILLEEEQKKERENERVRSHKESVQKLADELEF"
     gene            complement(2817..>3399)
                     /locus_tag="PW004_002646"
     CDS             complement(2817..>3399)
                     /locus_tag="PW004_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2727572.1"
                     /translation="SIDQNEQRQIEEMRKFGAEKIFIEKQSGATITHRPIFQEVLDFV
                     RDQDIFIVEAIDRLGRNYDEIIDSVNYLKKKNVRLIITNLPIMAEAIGNPLLDKFIKD
                     LIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANTKDPQRRLVYRSIV
                     QDQENGVAISKIATDYNVTRQTVYRIKKELTIN"
BASE COUNT         1244 a          473 c          603 g         1079 t
ORIGIN      
        1 cttattatga tttattagag gtttttgcaa gtgctaaaat cttggataaa aagaaaagag
       61 aggaagtaaa taaaattctg gagtcggaaa attctggtta cagattaagc gaaagcaatg
      121 aatttattcc aatcacagat gatatttcta ttaagtctat aaatgctgta tctgaatcac
      181 catttaatta cgcaaaaaat catatccata aatctcttac atatataagc gagaaggaaa
      241 atcaagatta taatactgta gtgagagaag caattagtgc tgtcgaaagt tgtcttatcg
      301 aattttcagg attaccagct aataaaaaaa tactttggga actgctataa aagcaattaa
      361 agacaaatcg aataacaatg tcgatcttac atttttaaaa ccatttgaga caatgtatgg
      421 tttggcgagt aataatggaa tacgacatgc agaaaatgaa aaaacaattg tctcagattt
      481 acctgatgca gttttggtgt taacaacatg ttcagccgta attaattatt tgggagtaaa
      541 aatagcatat gactccaata aatcatgaaa agttaaaatt catccaagag ctacattata
      601 actattaatt tccaactctt taaaagttta gtaattgaag gaagtaataa gggtgattga
      661 acaatacgga tatacggtgt atacttatcg taggagttga tgatatgaat attaaaaaac
      721 gcaaaatcag agaagtaggt aattcaatag ttgtaacttt gtcaaaagag agtttattgc
      781 aaaaaggttt gaaacctgga gatactattt ttattgatga agataaaatg atggaagcta
      841 ttgtaaaaga ggaaagcaag atagatcttg aaattgatat gtatgttaac caagctttat
      901 ctgagtatga tgcagctttc aaggaattag ttgaaaaatg atctatctaa gtgaaaaaga
      961 tattgtaaaa ttgaatgtta ttcaaataaa gaaatattca ccgcgagaaa caatcggagt
     1021 cattgacaaa aatgcgctga gtatggctgt aaatcaacct aaacagcata tttttggaaa
     1081 agatctgtat cctgataaat ttagcaaagc agctgttctg ctaataaatc tgatcaaaaa
     1141 gcatccattt cataatgcta acaaacgtac agcattttta gccgcgtatg tttttctcaa
     1201 gctaaatggc tattcactaa aaatgaaaaa tcaggaagta gtagaatttg tagttgaaat
     1261 tgctacgtat cagggtgatt ttgatgactt aaaagaggca acaacagata tactaaaaaa
     1321 gaattcaaca aagaccgatt caaaaaagta gaagcaaact agggattcag ttaaatctga
     1381 agatttagtt tgttctttat tttagtttac ataagataca ttatgcgaag ttcattattt
     1441 taaaagctta aataataaac ttttttataa taccaattta tttatgtaaa ccaatgacct
     1501 taatcgctta agcaagcatg ttatgtaata ctctactctc tccaaaacta atgactatct
     1561 attttcaaag aagctagaat gattatctga tcaaatgata atttatattt aattcctggt
     1621 ctactgctct tcttttaatc aattaatgtc taacgtactg tacacgataa agtaggaaga
     1681 cttattttca acttcggatt cattttttta attcggtgaa aatcactaaa ttaagagttt
     1741 atccaagttc attttagctg agaatacgct tgaaaataat tttgatgaat tttcctgaat
     1801 taaaatttat taacttgttg agtgatataa ttgtaatgta ttaccaaata gttagcactt
     1861 agattagtga tggaaaggaa tagtgagaac aagatgactg gtagaaatcc caaagtattt
     1921 atatcatatt catgggagaa taaggaaaat aaagaatggg ctagagaact agcagacaaa
     1981 ttacttgaaa acggtattga tgcatatata gatcagtatg atttaagttt aggtgataga
     2041 ttacctcagt ttatggagca gcaaatatcc gattcagatt atgttctaat tatatgtaca
     2101 ccagagtata aaaaaaaggc aaataatcgt acaagtggtg ttgggtatga aggtcatatt
     2161 atctcagcag aattgctttc tggaaaaaat gaacgtaaat ttatcccaat aattaaagaa
     2221 ggacgtgcaa gcgaagtact tcctactttt cttgctggaa agcttggaat tgatttatca
     2281 gatagccagc attatgatga aaacttagat gatttaatta caactatatt tggtgttaag
     2341 agaaaaccag cattaggtga tatacctagg catattaaag gcagtccttt actacctata
     2401 gaacataatg aaaataaaga tattgaaatc ttaggaatta tcactgataa agtaactgta
     2461 cctaaaatgg atggctcaag gggttctgca ttatataaaa ttccatttag attaaataga
     2521 gtaccaagtc agctttggaa agagttgttt gttagaaatt ggaattcacc accacgatat
     2581 acaacaatgc atagaccagg aattgcttct gttgtagacg atgaaatttt acttaatgga
     2641 acaactattg aggaagtccg ggaatatcat cgggaaactt taaaactttg cattgaaaaa
     2701 agtaatactc aagaaaaaaa aatattgcta gaggaggaac aaaaaaaaga gagagagaat
     2761 gagcgagtca gatcacataa agaaagtgtt cagaaattag cagatgaatt agagttctag
     2821 ttgatagtca gttctttctt aattcgatac acagtttgtc tagttacatt atagtctgtt
     2881 gctatttttg aaatagcaac accattctcc tggtcctgaa cgatgcttct ataaacaagg
     2941 cgacgttgag gatcttttgt gttcgcactg tataacttag gacgtccttt atagacgccg
     3001 tttgctttag caatctttat cccttgtgcc tggcgtcgtt ttgattcagt acgctcttgt
     3061 tcagcaatca tggccaagat ttgaatgatt aggtctttga taaatttatc caggagagga
     3121 tttccgattg cttcagccat gatcggtaaa ttagtgataa taagtcgcac atttttcttc
     3181 tttagataat ttactgaatc gataatctcg tcataattac gtccaagtcg atcgatagcc
     3241 tctacaataa aaatatcttg gtctctaaca aaatctagaa cttcctgaaa aatcggtcga
     3301 tgagtaatag tcgctccaga ttgtttctcg atgaaaattt tttcagcacc aaattttctc
     3361 atttcctcga tttgacgttg ttcattttga tcgatcgat
//
LOCUS       DANKKG010000187         3379 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000187 DANKKG010000000
VERSION     DANKKG010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3379
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.189"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            138..938
                     /locus_tag="PW004_002647"
     CDS             138..938
                     /locus_tag="PW004_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HDL2727573.1"
                     /translation="METIIQIMKKYDFSEMETRVYTTLLEKGNLTGYEVSKISGVPRS
                     KVYNILEKLLKKNLIVVNKSEPKLYHAISANEFLEKLEKSVKNDLSFLTKNLGMIKEK
                     DEEEMLWKVDGIEYVLDKVEHLVKNAKESLLIQVWHENFTDSLLKALQQAEKRVDKFV
                     LILFSSTHEYDLPLEKYYIHGFETDKLADFGARWINIVADEQEVVFGTINEELQSTDV
                     TWTKNHAMVNLAKEYVKHDAYTLKVIAESPEELKAKYGEGFEGIRKIY"
     gene            954..1832
                     /locus_tag="PW004_002648"
     CDS             954..1832
                     /locus_tag="PW004_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDL2727574.1"
                     /translation="MIRNILLTLIVAINLYFIIYFLRDLFKNKQNFKEEPGDMRLLPF
                     TSFIIFFLSTFGVSDFAIGTVLYPKLKWVSMKKLPGTLNTQCVVPVAVMALSYITAIN
                     VGIKTLAVCIICQIIGAYLGPRFVVKLPEKTIKLFVGIGLVIAALLIFAGQMNWIPSN
                     GTASELYGGKLILAGFLLFVYGALNNIGIGSYALTMVTVYLLGLNPVAAFPIMMGACT
                     FSVPIGSVQFIKFNEYSRKITLFTSTFGVLGVLVAVFLVKSLDTYILKWIVILVLLYS
                     AYTMLGSQLKKVTAAN"
     gene            1845..2825
                     /locus_tag="PW004_002649"
     CDS             1845..2825
                     /locus_tag="PW004_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HDL2727575.1"
                     /translation="MLFLKKEDIIKSFSMREAIDADKKALSLYSAGKASVPLRTNIDV
                     PKSNGQSLYMPAYVEGGEGALGVKIVSVYPENIKKNLPSVPATMIVLDPETGMVSACL
                     DGTYLTQLRTGAVQGAATELLAREDAKIGALIGTGGQAQSQLEAMLTVRKLEEVRIFD
                     IDFERASQFAEKMMQQFSVTMRPTKTSQECVEGADIITSVTTSKRATFSAEWVKKGAH
                     INGVGAYTPEMCEIPREIIKAADVVIFDTMDGVLKEAGDFISPLQDGYIQRDSYHGEL
                     GQLINEELIGRTSGEQITIFKTVGSAVLDVVVATEIVKKAKENNLGKLIF"
BASE COUNT         1171 a          545 c          699 g          964 t
ORIGIN      
        1 atgtttcttt ttaataaaaa gattgttttt aagctaatta ttatatgtgt gttattgaac
       61 taactcttga cagataatca aaaagaattt atagttgtta ccgtagtagc tactaaaaat
      121 aaagtgagga ttaatcaatg gaaacgatta ttcaaatcat gaaaaaatat gatttttcag
      181 agatggagac aagagtgtat accacactat tagaaaaagg taatcttaca ggatatgaag
      241 taagtaaaat ctcaggtgtg ccacgctcga aagtatataa tattttggaa aaactattaa
      301 aaaagaattt gattgtcgtt aataaatcag aacctaaact ttatcatgcc atctcggcaa
      361 atgagttttt agagaaatta gaaaaaagtg ttaaaaatga tctttctttt cttaccaaga
      421 atctagggat gatcaaagaa aaagatgaag aagaaatgct gtggaaagtt gatggtatcg
      481 agtatgttct ggataaagta gagcatttgg tgaaaaacgc aaaagaaagt ttgctcattc
      541 aagtttggca tgagaatttc acggattcac tactaaaggc attgcagcaa gctgaaaaaa
      601 gagttgataa gtttgtactg attttattca gttctaccca tgaatatgat cttcccttag
      661 aaaagtatta tattcatggt tttgaaacag acaaacttgc tgactttgga gcaagatgga
      721 tcaacatcgt cgccgatgag caagaagtcg tgtttggtac cattaacgaa gaactgcaat
      781 caaccgatgt cacttggaca aaaaaccacg ccatggtcaa tctagcaaaa gaatatgtca
      841 aacatgatgc atatacacta aaagtgattg cagaatctcc tgaagaactt aaagcaaaat
      901 atggtgaggg ctttgaggga atcagaaaga tttactagta ggaggaattt tttatgattc
      961 ggaatatttt gctgacacta attgtcgcaa tcaatctata ttttattatt tactttctaa
     1021 gagacctttt caaaaacaaa caaaatttca aagaagaacc aggggatatg cgtctgttgc
     1081 ccttcacgtc atttatcatc ttctttcttt caacatttgg cgtttccgac tttgctatcg
     1141 gaaccgtcct ttatccaaag ttgaaatggg tcagcatgaa aaaactaccc ggaacgttga
     1201 atacccaatg tgtagtaccg gtagccgtca tggcattgtc ttacatcact gccatcaacg
     1261 tagggatcaa gacactagct gtttgtatta tctgccaaat tatcggtgca tatttaggac
     1321 caagatttgt cgtgaagtta ccagagaaaa cgatcaaact gtttgtcggc ataggattgg
     1381 tcattgctgc attgttgatt tttgcaggac aaatgaactg gattccatca aacggaacag
     1441 ccagtgaatt gtatggcgga aaattaatct tggcaggatt tttactcttt gtctatggtg
     1501 cattgaacaa tatcggcatt ggttcttatg cgttaacgat ggtaactgtc tacttgctag
     1561 gcttgaatcc agttgcagcc tttcctatta tgatgggggc ttgtaccttc tcagtaccga
     1621 tcggcagtgt tcaattcatt aaatttaatg aatacagcag aaaaatcaca ctttttactt
     1681 caaccttcgg tgtattgggg gtattggtcg ctgtattcct agtcaaatct ttagatacct
     1741 atatcctgaa atggatcgtg attttggtct tactatacag tgcatatacg atgttgggca
     1801 gtcaattaaa aaaagttaca gcagctaatt aaggagcgat tcaaatgtta tttcttaaaa
     1861 aagaagatat tatcaaaagt ttttcaatga gagaagccat cgatgcggac aaaaaagcac
     1921 tgtcattgta ttcagcagga aaggcaagtg tgcctcttcg caccaatatc gatgtgccaa
     1981 aaagcaacgg acaaagctta tatatgcctg cttatgtaga aggtggcgaa ggcgcgctag
     2041 gtgtcaaaat cgtttccgtg tatcctgaaa acatcaagaa aaacttacca agtgtaccag
     2101 caacgatgat cgtattggat ccagaaacgg gtatggtatc cgcttgtttg gatggtactt
     2161 atttgacaca attaagaaca ggcgctgttc aaggagcagc aacagaactt cttgcaagag
     2221 aagatgctaa aattggcgcg ctgattggaa ccggcggaca agcacagtca caattagaag
     2281 caatgttgac cgttcgtaaa ttagaagaag ttcggatttt tgatattgat tttgagcgtg
     2341 ccagccaatt tgctgaaaaa atgatgcaac aattttctgt aacgatgcgc ccaacaaaaa
     2401 ccagtcaaga atgcgttgaa ggagcagaca tcattacaag tgtgaccact tcaaaacgtg
     2461 cgacattctc tgctgagtgg gtcaaaaaag gtgcgcacat caatggagtc ggtgcttaca
     2521 caccggaaat gtgtgaaatt ccacgagaaa taatcaaagc cgcagatgtt gttatttttg
     2581 ataccatgga cggtgttcta aaagaagctg gggacttcat ttcaccgctg caagatgggt
     2641 atattcaaag agacagctat catggggaac taggccaact gatcaatgaa gaattgatcg
     2701 gtcggacaag tggagaacaa atcacgatct tcaaaacagt tggttcggct gtattagatg
     2761 tggtagttgc gacagaaatc gttaagaaag ctaaagaaaa taatttggga aaattgattt
     2821 tctaattaaa aaggctgaaa ttaaaacctt tattattgcg tttaaaaaaa gaaaacaaag
     2881 aagtttgtaa tcaaatggaa actgacccca taatttaaat aaaataaaaa acatctcttg
     2941 ttgaattcta gtaatgaagc gaccctaaaa cttagaccaa aaaatctaac ttttgggtgg
     3001 agttgtcaag aaaaaccggg cacttagtta aaaaataagt tggacgggtt atactaaaat
     3061 tgacaccaaa ttaagagaaa gcgaagatct ggattatgcc aagacgttca tttgatgaaa
     3121 tatttaaaat taccgctgta aaactggtaa cggaagaagg gttttctgtt aaagaagtta
     3181 gtctatagct agaagctcgc tctaatagcg tgtattgatg agttcaagaa gttgaaaaat
     3241 atggataacg tacttttctc ggtagaggaa gtcatgaaat ccattcgcag aaatgaaatt
     3301 acacgcttag aaaaagagaa taaacggtta aaagaatagg tagaactcct aaaaattacc
     3361 gaagtcttcc tgaaagaaa
//
LOCUS       DANKKG010000188         3370 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000188 DANKKG010000000
VERSION     DANKKG010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3370
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.190"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(240..1151)
                     /locus_tag="PW004_002650"
     CDS             complement(240..1151)
                     /locus_tag="PW004_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDL2727576.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(1528..2166)
                     /locus_tag="PW004_002651"
     CDS             complement(1528..2166)
                     /locus_tag="PW004_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2727577.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDFVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFNQETKHLTGREKRLIKRNALSFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKEQVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(2170..2940)
                     /locus_tag="PW004_002652"
     CDS             complement(2170..2940)
                     /locus_tag="PW004_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HDL2727578.1"
                     /translation="MIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQE
                     ISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSTYWERNILSGEIYPPLK
                     IKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFS
                     FFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIER
                     EPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
BASE COUNT         1017 a          616 c          510 g         1227 t
ORIGIN      
        1 agaaatttgc aaaaccactc catttatcta cttctacgtg cattttttaa tatcgttttc
       61 tttatttaag attatacaga aaaatatcta gggtggctgg attctaagta aaaaaaattt
      121 ttaaaataaa aagaactaaa tcaagtaata attaaactac tgttgattta gttctaataa
      181 aaagatttag atgaacagtt ttattgattt aaatagttca tattttttat ttataccgct
      241 tatttactac ttgctgtact agaatctgaa gtgaatgtac tcaaaacatt ttcaaagtct
      301 ttgtctttga tttttacatt agcgtctttc aatacttcgc caattacttt attctggaag
      361 gtactgtcac ttaattttgt gtctgttgca atttctttca attctttttt gtatttatcc
      421 atgtcgttgc ctttgttttg gtttttcacc attttaacca cataatattc cgtagtataa
      481 gttgaagcat tggtagaagt gatcacatca gaaacttgac catctttcaa cttaaaggct
      541 gcttctttga cttctgccgg aaccgttgta gtagtggaat caaatttcac ttttccgcca
      601 tcttcttttg tcgtagtatc agttgacttg tctttggcta atttagagaa atcgtctccc
      661 ttatcagcag ctttcttcac atcttttgca tcatcttcac ttgctacttg gataatttga
      721 gcttccactt ctggatggaa agattcccaa gccgttttta aatcttcatc ggtcaatttg
      781 atatgtttct ttagaccttc ttgaaaggct agactttgtt tgatactgtc tttgaatgtt
      841 tgttctgtgt aacctgcaga tttcaattgt gaatcaaatg aatcacccaa ttgtttcttt
      901 gtttgatcat attgggaatc gatttctttg gttgatacgt catctccata tttatcttca
      961 aatacttgat aaacgatcaa ctgttgcaca gcggaatctg ctccttgcat ttttttcaag
     1021 ctatccatta cttcttgttc gctgattgta ccgcctttat aactaacggc tgttttttca
     1081 ccggcattcg tacagcctga caataagcca gtgcctagta cgatggcaag tgctaaaaat
     1141 ctttttttca tttatcaatt ctcctacttt tcgttttttg cgaattgcta aaaacaaagg
     1201 tgttcaagat gaaaatcatc aagaacacct ttactaatct gctcgatacg tcctgaatag
     1261 atataaggct acacaatcca gaaagttcgc tcagcagaat aaatgtcaag tagcacatca
     1321 caccttaata tactacttct tttgttaccg tttttcaagt ctatcttgcg ttccgtattt
     1381 aacgtaacta attcttagaa caaatgttat cgattttgtt tttcttcttc cgctgttggg
     1441 atattcaaat cagacacttt tatgttcgat tttccagaca tttttttgtt gtttttatta
     1501 gaaaaagttt gttttttcat ttgttttcta aagcatgatc ttcttgtttc ctattttggt
     1561 cagatatgga tcatgcgtga ccacgataac taacgtacct tgggaagcct gttgcgataa
     1621 gatttcaacg acttgctcct tatttcgttt atctaatgat ccagttggtt catcggctaa
     1681 aatgattttg ctattcttca ataagactct cgcacaggcg attctttgct tctcaccacc
     1741 agataatgtg tagataggcg tacttagctt cttgtttata tttacttttt tcaatgcctc
     1801 atactttgct gttcgcttat ctgaaacatt taaattcgaa aacttcaatc caatatctaa
     1861 attaaaatcg attgtttcat attcaatcaa cccaaaatct tgaaaaagaa aagagagggc
     1921 atttcttttt atcaagcgct tctctcttcc agttaaatgt tttgtttctt gattaaataa
     1981 ataaaattgc cctgaactga attgatcatt caatcctaat atattcaata aagtactttt
     2041 ccctgctccg ctctctccaa ctacagtcac aaaatcccca gcatccgcag tgaaacgatc
     2101 caaatgaaga atcgttttgt ttcgaatgat ttttttcgca ccttttatca ctatcaacgg
     2161 ctccaattgt taacctccat tcatcagcaa taactgactt ttcttttctg tagacaaaat
     2221 aaaaacaact gaaagaataa aatcaatgaa gctaaagaaa aaaatattaa aaagatattt
     2281 aggctctctt tctataagaa aaaggaagat tacacttgtc gttccccatt tgatgaggtt
     2341 catcatgaag aaatctttat acgtttcaaa gagcgaataa ccacttgaac gaaagatagc
     2401 gattttccga caattgtaga caaaatatat gcataacatg acatagctca acaagaagaa
     2461 agaaaagaaa gtgagtaaaa atatctgtat caagtaatga aatgatctgc ttaacacttt
     2521 gacagctatc tcttcagtct gagtatacgg aaatgataac tgcaagttat cacgtaactg
     2581 actttcttca atcagctcaa ataacctttc tttcgacaaa gagcctatct tgatctttaa
     2641 cggtggatag atttctccgc tgagaatatt tctttcccaa tacgtactgt ttttcaatgt
     2701 cagtatttca acaaccggat aatgttcgat ccaggggata gaaggaataa atgtataaat
     2761 gggttgattg ttttttataa atagaaattt cggttctttt tcactgattt cttgataata
     2821 ttctgttatt tgcgccaggt cattttttaa atctgtatgt ctatatcgtt cagggatcaa
     2881 tataacaggg tgcgtttctt tttcggttat ttggatcaac cttccagaga catcaatcaa
     2941 aggaaatttc ttaagataat tcagatttat ttttatccct ctgccaaact gttcattttc
     3001 ttcattcaag taatctgata cattgactaa tatacttccg tttttattca aagtttcata
     3061 taattcctga tcggctgctg atgcaaaacg attggaataa ataaaatcca cgtggttttt
     3121 cccgatggtt actggataaa atacatgata gtcctcacca gcgatttctt ttggtgtttc
     3181 atgggaaaca gctaactgtg aagaagaaaa aacgatagtt gctaattcga cattggcaac
     3241 ggtcaaaaga agagaaaaca acaatccaaa tgtaaaaaga atgtattgga tatagttgaa
     3301 cgcttctctt aacgaagcga ttttttcaac taataaaaga gaaatcaaag agatacccta
     3361 tattccgctg
//
LOCUS       DANKKG010000189         3361 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000189 DANKKG010000000
VERSION     DANKKG010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.191"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            155..1921
                     /locus_tag="PW004_002653"
     CDS             155..1921
                     /locus_tag="PW004_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HDL2727579.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2023..3282
                     /locus_tag="PW004_002654"
     CDS             2023..3282
                     /locus_tag="PW004_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL2727580.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
BASE COUNT         1192 a          549 c          719 g          901 t
ORIGIN      
        1 cttttaaaag ttttaagtct atctatttgg caagtttaaa aaataaaaat tcggtatcaa
       61 aaaactgtta ccgataacat ttttgaaagc gttgtctttt gtttctctat cacttatagt
      121 gatgacgtca tattaaaaca caaggaggaa atcaatggat acagctgcaa tttatcatcg
      181 accggaaagt gaatatgcat ttctatatac gaatacaaga tttcgtattc ggcttcgtac
      241 taggaaaaat gatatcaaaa aagtatatgt gctttgtgga gatccatata ccataacaac
      301 tgaaaaatgg tatcaaaaac aacgaccaat gaaaaaatgt ctaagtacca atgttcatga
      361 ttattgggaa attgaagtga cgagcgaaac aaggcgctta cagtatgcgt tccatgttgt
      421 tggggaagat aacacggact gtttttacgg tgatcaagga atttttcctt atcgagaaaa
      481 tgtgattact gaaccaaatt tttattttcg aataccttat tttcatcaaa tcgatcgatt
      541 cactattcca gaatgggtaa aagaaacagt atggtatcaa atttttcccg aaagatttgc
      601 aaacggggac aaagcaaatg accccgaaca cactttacct tggggaagta aagatccaga
      661 ccgagaagac ttcttcgggg gagatttgca aggtgtgagg gatcatttgg attacttgac
      721 ggaccttggc gtcaatggca tctatttttg tcccatattt aaagccacat ccaatcataa
      781 atacgatacg attgattatt atgaggtgga tcccgctttt ggcgataaaa aacttttaaa
      841 aaacttgatt gatgaagcac ataaaagagg gatacgtatc atgttcgatg ctgtattcaa
      901 tcatatgggt gtccattcac cacagtggca agacgttctc aaaaacggag aaaaatcgat
      961 ctataaagac tggttccaca tccgtttttt cccagtagat tcttatcaga tgactgactt
     1021 accagaaact gctgaaaata tcccatatga tacttttgct tttactccat tcatgccgaa
     1081 actaaataca gcaaatccag aagtgcaaaa atatttgctg gatatcgcaa cttattggat
     1141 aaaagaattc gatattgatg gatggcgtct tgatgttgct aatgaagtcg atcatcattt
     1201 ttggaaaaaa ttcagagaag cagttacaga aatcaaacca gacatctata tccttggaga
     1261 gatttggcat tcctctcaag cttggctgca aggggatgaa ttccatgcag tgatgaatta
     1321 tgcattcaca gattcgatta aagattattt tgcaaaaaag aaaatcacag cttcacaaat
     1381 ggtcagtgga atgaatcatc agcaaatgct ttacagagat caggtgaacg aaggaacatt
     1441 caacttatta gattctcatg atactgccag gatcttgacc ctttgccaag gtaacaaaga
     1501 attgatgaag tctgtgatgg cctttatgtt tcttcaaaaa gggtctcctt gtatctatta
     1561 cgggacagaa atagggatga ctggtgaaga tgacccggat tgccgcaaat gtatgatatg
     1621 ggagaaggag gaacaagatc tagagctatt cggttttata aaagaacttg tttctctaag
     1681 gaaacagttg tctaagatta tttctgaagg ttctactcag tggctaatag tatatgatcg
     1741 tgaagataag ctctatttca cacgggaact agagggacag atcatctatg tttactttaa
     1801 tcagtcaaaa gaaccttggg tagtagaaca ggaaaatgag gtgattctta gtcaaaattg
     1861 tcagttgtta gaagacggga aagcagagat acgacaatac ggatttttga tcatgtgttg
     1921 aaaactgtta ccggtaacaa caagaaagcg tttgcaaatg aagatttgtg cccattataa
     1981 taagggtgta acagcacgtt gaatacacag gaggaaatga agatgaaaaa aagaagtgtg
     2041 aaacagttgg gtttagggtt aatctcttta ggaatcttag gcggattagc ggcttgtggc
     2101 aatggtaact cttcagcaga aggggaagcc gatgaaggaa aaacgttaac agtttccgtt
     2161 gattctggat ataaagatta tataaacgaa ataaaaaaca ctttcgaaaa agaaaacgat
     2221 gtgaaaatca agctagtaga aaaggacatg ttcgatcagt tggaatcatt agcgttagat
     2281 gggcctgctg gaaagggacc tgacgttatg atggcagcat atgaccggat cggtgcgtta
     2341 ggacagcaag gacatctggc ggaagtaaaa ctgggaaatg aagcagctta cgatgaaaca
     2401 gacaaagccc aagtgactta tgatggaaaa atctacggag aacctgcagt aatcgagacc
     2461 cttgttttgt actataacaa agatttagtc gatacagcac catcaacgtt taaagatttg
     2521 gagaatctat ccaaagacag tcgttttgct tttgagtcag aagcaggaaa aaatacagga
     2581 tttttagcta aatggactga cttttattat tcctatggtt taattgcagg ttacggtggc
     2641 tatgtatttg gtgatgatgg cacaaatcct tcagatatcg gtttgaataa tgcaggtgct
     2701 gtagaaggaa tctcttatgc aacagattgg ttcaaaaatg tttggccaaa gggaatgcaa
     2761 gacataaaaa gtgcaggaga cttcgccagc cagcaattca tgtccaataa aactgcagca
     2821 atcattgacg gtccatggca agcgcaaaca tacaaagaaa acaatatcaa ttatggtgta
     2881 gccaaaattc ctactctaaa taatggacag ccttatcaac cgtttggcgg aggaaaagga
     2941 tgggtagtca gcaattatgc taaaaacaaa gacctctctc aaaaatggct tgattatgta
     3001 acgaatcagg aaaatcaaga aaaattcttt gaaatggtaa atgaaatccc tgcaaaccaa
     3061 caagctaggg aaacagcaaa ggctaaaaac gatgaattaa caacagctgt gattgaacaa
     3121 tatgaaacag ctcaagcgat gccaaacatc ccagaaatgg gcgaagtttg gacaggtgca
     3181 gaaaacttga tgttcgatgc agcatcaggt tcaaaaacac caaaagaatc cgcagatgaa
     3241 gcagtaaaaa caatcagtga agccatagaa caaaaatact gattaaaatg ataaccgtcc
     3301 atttttataa aaaatggaac aggtgcttca gataaaagtg gcaagagcga gatggaaaat
     3361 t
//
LOCUS       DANKKG010000190         3331 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000190 DANKKG010000000
VERSION     DANKKG010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3331
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.192"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(456..1766)
                     /locus_tag="PW004_002655"
     CDS             complement(456..1766)
                     /locus_tag="PW004_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HDL2727581.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1778..3196)
                     /locus_tag="PW004_002656"
     CDS             complement(1778..3196)
                     /locus_tag="PW004_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2727582.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
BASE COUNT         1029 a          675 c          514 g         1113 t
ORIGIN      
        1 gtttattgtc ttgaattgaa tcaagacgcc ctacacattt ggtttgtctt actttgttca
       61 gttttcaaag gtctacttca tcgagagtgt ttcccgattc atttttggct gccgtagcaa
      121 cttttatatc atatcagaga gacaaacgat tgtcaactct tttttaaaag tttttttcga
      181 tttcgttttt cgaaatcttt cagcaactca tttatcataa cttatcgttt gttatttgtc
      241 aagaactttt ttgaagtttt tttcgataga atcaagcttc gcaagtggtt ctttttaacg
      301 acttagttat cttatcacgt ctaaaaacat gtgtcaactc ttttttggaa ttttcataaa
      361 atgtttcaat gaagcgcgac aacgttgttt aatatatcat taaaaaaaat aaaggtcaag
      421 ctaaaatatt atttaagctt gaccttatta taatttcaac gattgtcaaa agtattgttt
      481 gcagctgtgt tgccagtttc tatagataaa tcacagcttt tgaaacataa gttttgtact
      541 gcttcgattc gcccgaattc ttttgcagtt atttggcaat gagaaaagca gacatcttct
      601 ataggctttt caggaaatgc tactaaatca aaagctcggg aaggaagtga gtaacctgga
      661 gaaaggcatg cctgtacgtt tttgatgtgt atgtctttga ctttcgtcat ttgctttgat
      721 tcaggtattt cttcagccac tgcttgccaa ctttctggca tttgagcaag gtcattgcta
      781 ttttttctat tgtattcatt atgccagtcc attatccaag aaaaaggaaa ttgtacatca
      841 atcatattca gattttctac cctgatattt tcgatcactc cccctctttc ttttgaagat
      901 ttcattcgaa taccacagtc tgactgaaaa aaatcgatat cgtgaatata tacgtcacta
      961 actccagcac taacttcgct accaatagtt acaccgtatc ctgaacggat tttacatcta
     1021 gcaacctcga ttcttgatga ttttcgattg actcttgcac cattgccatc tctgccagac
     1081 ttaatagcaa tacaatcatc accacaggcc aaatcacatt catagacacg cacatttgtt
     1141 gaagaatcaa tatcaatccc atctgtactt ggtccatcat tgttgcggat gattactttt
     1201 tctactaata catcgttaga ataagtgatt tgcaagttcc aaaaacctga tttctgaaaa
     1261 atcaaatcac gaacaacaac atgttctgca tgatacagta aacaagcgcg tggacgttta
     1321 atagcataat ccgcaatcca acgaagtccc ttttgatcgt attcagctct tgtcccgctt
     1381 ttttgatctt ttccccaata cagctcccac caatggtctc cttgaccgtc gattattcct
     1441 tttccttcta taagaatatc tttcgccgag agaacattca aaatcgctgc tggccatttc
     1501 atttctacgc cggctactcg tgtatcgatt tctggaaatg cttcgatatc tgttctcccc
     1561 ttgaggactg caccttcttc aaagataaga tgactcttat cttttaaaaa aagggctccg
     1621 actaaataaa tgccttttgg aataaataca ggtaagtgtt ctttcgcacc ttgatcgatt
     1681 gctttttgca gtattattgt atcatcttgt tgagtgtcgt ttgttttaaa gttcaaagca
     1741 ttgataatcc ctttttttgt ccgcaatacg tttctcccta tctttcattg atttttttca
     1801 attcttcacc aataaataca gtttttgaac gattcagtgg gtaacctact agcaaaatga
     1861 ttgcggatac aaataagcag aatgctggta acaagtttgc catcgtataa atcctttctt
     1921 gtactaaaac tgattggatg gctccacctg ttgtagacga ttggtaacca atgagtgcca
     1981 gcataaatcc gcctaccgct cctgcacatg cttgtcctac ttttctcgca aaagaattta
     2041 ctccatatac tgttccatct tctcttaagc ctgtcttata ttgatgataa tcaatcacgt
     2101 cagtaatgaa tgcccagacc atcaaattga ataaaccaga tcctaaagta gcgataaata
     2161 aaagaatcag atatacccat gcatttgtta tatgaaggaa atacatcagc aagtacatac
     2221 atgaagaagc aagtaacgca aatacgcttg cttccttttt accaaataat tttgtcagct
     2281 ttgtagcaaa tggtgctaaa actactactg ttccgtaagt aaataaaaga gcaacagaca
     2341 ttgcttgttt attatggaag taatcattga ataaataagt catattcgtc ccagctaaaa
     2401 tctgattgat cacaatgaaa atgtcaacaa tgactaaaat aatcagtgct cgattagcta
     2461 acaaaccttt taccaactga gaagcagata ctttttcgtt tttgacaact cttactcttt
     2521 cagtagtcaa tttacaagtt aacatatatg cgacaaatgc tattgcagca catccgattg
     2581 aaatccaaaa gaaatgattc cctgaaacga cttgttgtcc actcttgtta tgggtataca
     2641 ttaaaatcgg aatgaagaat cctgtagttg atccgccaat cgcagaacca atgcttcgga
     2701 acgtggacaa cgaggtgcgg tcattaggat ttgagctaat tgctgctgcc attgctccat
     2761 acgggatatt gattgttgag agaaaaatcc cataaacaat gtatgaaaca aaaatataag
     2821 caatcttcac cgtcattgga agatgttgga cgaatggcaa aaacaaaatg acagtcacta
     2881 agcaaaatgg atacttcatt tttctgatcc aaggaataaa tcggccagtt tctgttaact
     2941 ctgagacatc acataatctt ccaaccatca catcagcaaa cgcatccaaa attctcgaaa
     3001 tcaagaataa cgtacctacg attgcccctg aaacgccaag aacattcgta taataaatca
     3061 tcaagaaact attgactaaa cctaaaataa aacagttacc tagatcaccg aacatgtagc
     3121 ctatcttatc ggaaatccca aattcctttt tttctttttt tattgtattt gctgtaacag
     3181 cattttgtga aagcatttga attcctccta cctcttctag taaacgtttt caaaattata
     3241 actctttttt tcttgttttt atattcacaa tgttggcttt ttattgcatt atcttgacag
     3301 ataaaaaaca aaaaataatc taaaaacatt g
//
LOCUS       DANKKG010000191         3330 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000191 DANKKG010000000
VERSION     DANKKG010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.193"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(<1..2303)
                     /locus_tag="PW004_002657"
     CDS             complement(<1..2303)
                     /locus_tag="PW004_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727583.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTGLTTGEDGKLAIDGLAPGAYQLVET"
     gene            complement(2975..3268)
                     /locus_tag="PW004_002658"
                     /pseudo
     CDS             complement(2975..3268)
                     /locus_tag="PW004_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          907 a          745 c          547 g         1131 t
ORIGIN      
        1 gtttctacca gctgatatgc tcctggtgct aaaccgtcaa ttgccagttt tccatcttct
       61 cctgtagtta aaccggtttg tagcgtttct ccttctcggt tctgtaactc aaaaacagct
      121 ccttgaagga cttcaccact gccttcagca tgtttaatta gttcaacacc acccgtatca
      181 aggatgtttt caatggttaa cgtcacttct tctatggagt attcatttat aaaaacatca
      241 tgtaccgtcg catctaaaag atacccttcc ggtgcttctg tttctcggac ttgatagcta
      301 cctacaggaa gcggttcact taaaaattct ccgttttctg ccgttatacc ttcttgtatc
      361 acttgtgaat cgcttgataa aatttcaaat tttgctcctg ccagcctttg gccattctca
      421 tcagttttaa ttactttaat acgcccatcg attttccttg ttcttacagt actcgtattc
      481 aatcgattgt cactattcac aagcgttgca aagtttaaaa tttgatcgcc gaccgcatac
      541 gcctctacat tgaccttaaa agagagggta aaacttgtcg gaccaccaat cgtattattc
      601 gcactgaaac gaactattcc attattgact tcggcatcaa tgaaagaggg aatatctgat
      661 agaaatgaca caaattccat gccttcttcc aaaacatctt caataacaag cgtatttctt
      721 gttccagaaa acatcgagac atgcagagta tacgttagtt cattttcatt gagattgata
      781 acatttttgt ccacactttt tcttaaagag attgccggaa gattactttc agggattctc
      841 aagcggtagt ctcggctcat tacctgatta ttgtaggtca tactcgctcg attccgagaa
      901 aacccatcct cttgtcttcc ttcattgaac aagtcgacat cggtcacttc aagagcatat
      961 tcaataaaat acgattgacc ctctgctaga ttaccaaagt ccacaacaag tgtattcgtt
     1021 tccttattaa aagatacttt gtccacaaat tcctcggaaa cataatgagt tctaacgttt
     1081 gcttcacctg gattcgttac ttctgcgatc cgaaccccac ctgaaatagg ttctctattt
     1141 ccagtggaac tccatgtagt cggattcggt gccaaagttg ttccttgcgg tagtacatcg
     1201 gtaaccacta caccgtcttt tcgtgatcct tcataattta cctgcaaaat aaatttatta
     1261 ctgttggtca cgtcttgccc gtcaatcagc agattcccaa gatttccttg gctgtcgatt
     1321 tctctagagc catagtataa tttcgaaaaa atgggtcttg atgaaggaat aggcgtaatt
     1381 gaaaattccg tgtttgctgc tcgctcagca taagtaagac tcacttgaaa gtcattattt
     1441 ggctcagtac cgcctgattg aaagaaagca acttcaaacg tgatactgaa tgtatacgac
     1501 tgagcggctt catttaatcc ttcataatca aaggtaaatg gaaaaacaac gtctgtgcct
     1561 tcttggtaaa atgccagtgg ctttaaaggt gaatcatcag cgacctccac tctaatgaac
     1621 tcaattaagc caatcgatgc tctaggaact cgtacgacca tctccccata ttcttcaaga
     1681 atatcgccag cgcctccttg aatgtatccc tttaacgttt gtgtatgcct gctcggcaaa
     1741 gtggggcgct cagcagagat gccgatgtca taattcaaga gctcctcttg tctttcaatc
     1801 tgagattcct gcgttataga tggctcagat gatctggctt cattaaaaga ttcacttaag
     1861 ctttcttctg gtatattaaa agattcactt gtgctttctt ctggtacatt aaaagactca
     1921 cttgtgcttt cttctggtac actgatttct attgagaccg tatttgattg aatatcaccg
     1981 cttcttgcgg tcaattcgta tttacctgaa tgctgggaag aataagtaat cagacgttcg
     2041 taatcattga gttgcttcac ttccccttca atcaaatcct cactcttatc aatgaccaat
     2101 gcatttagca ggtctggtgt tcctctgact cgcaactgga actgctcacc caaagcagcc
     2161 tcctcttgat caacttcgat cgtcagcgaa cgctctggct gttctgctaa agactcagct
     2221 agaaccgtta tgttcccaaa ttgattgatc acaataagca taactagaaa catacttaac
     2281 agtttatgaa atttgttcct cactattact cccccttata attttgacta aacaacaagg
     2341 tacccgataa ttttctaaaa agattcccca actcaaaatg gctgcgttac ctccctcgta
     2401 ttttcggttc aactcaagcg ttgcaatgta agctgtaaac aaagctaatg attgccgatt
     2461 gtcttctgtt gattgattcg atagctttgt atgactttta gttgtccgtg tggcttaacc
     2521 ataattatga tttcacgatt cctcctgcca gtgccttgca caagctggag cgtaacccta
     2581 atagttattt tgttaatgta cttatatact tttatacttt tccattagaa acaatcacta
     2641 aatcgttgat accaaggtca gactctagaa tttcatcagt gagtccctgt attggttaac
     2701 cttcatcaat cccaaaaaaa tatatcaatg cttagcatca aaatgaatat atagtttaga
     2761 atacttggga ttcatcgata actgatcgct tatttggcca tattatttgg cacttggttc
     2821 aactaaacgc atttaggatg ttccttcatt ctcgtcatgg atgttctcaa accgtcatta
     2881 ctaataaact gtccaccgaa tcgatggttc atttcttgca ttttccacgt ccaattactc
     2941 tttttgctct ttatttgggc gtagtttcac tttcttaact tgtatcgtct tccatcaact
     3001 ccttaatcat cttcttagct ttattggctc ggtttccttg taaaaggtat ctacaaatag
     3061 tgacaatctg tattaaatct tcatcatttt ctgtaatgtc aataatttct atattctgta
     3121 ccatatattg caaagggttt cactaagttc ataaccgaag cgaattaaac aacttttgta
     3181 tagaataact accttttcta tttttccatt ggtaattcta tctattaatt ggtttaatcc
     3241 ttttgttgta gttgattcca ctaccaagat gattctctcg aattttaaag cttgaagatg
     3301 tattgctatt tttgctaact ggcgtatttg
//
LOCUS       DANKKG010000192         3327 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000192 DANKKG010000000
VERSION     DANKKG010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3327
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.194"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(<1..506)
                     /locus_tag="PW004_002659"
     CDS             complement(<1..506)
                     /locus_tag="PW004_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2727584.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQVILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSM"
     gene            778..3249
                     /locus_tag="PW004_002660"
     CDS             778..3249
                     /locus_tag="PW004_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDL2727585.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
BASE COUNT         1075 a          601 c          670 g          981 t
ORIGIN      
        1 ttcattgatt tgaactcatc catacacaac accgtcggaa ggaagttcat acgtggctgg
       61 taatgcttcg ttgcttcacg caagatccgt gtcaccgtcc catccgagac gaagtacttc
      121 ttagcgatta gtttacgtga ttgattttcg tacagttcca tgagaatcac ctgcttcagt
      181 tcctttgtta gttgatgatg ttcatcaact aagtccgttt tcgcagaaaa ggtccttcca
      241 caatcatggc agagaaaacg ggtacgtttt aattctaaaa tagttaactt ttctttgact
      301 ttcaacatct gagtgcgtgt cgtataggaa ccgttgcgga taatacgaac cgatcgacag
      361 tgttcacaat aggaagaaga gtcatccaat tttcctttaa taaactgcgc ttgaacgcct
      421 ttatattctt tttcaatcag ccattcttca tcaaaaaata aatgtttatc tgttaatccg
      481 agtaattttc tagtatgatt atccattgag agactccttt ggtaggatga cgttttggtc
      541 gactaaattt taccaaaagt gagtctcttt ttgagttgaa acgaagaaaa agtgttagta
      601 gaaaatcgcc ttcgattttc caccaacaca aaatattata gaaccctttt ttttgctata
      661 tgacaggaat gaaaggtgtt tgcaagctta atgtgaggaa cactttccat atctgttcta
      721 tactggatag gagaagtttg gaaatatcca gaaaacgaaa tcaatcgagg tagccacatg
      781 aagaataaat ggataaatcg aagtaaaaaa aagaaagtaa cacaaagatc atcagataaa
      841 gtcatccgtc tttttccatc gtataaagaa ggattaacgg cagatcaagt caaggaacga
      901 atagaaaaag gtgcagcaaa taattcagtc gatcccacat tcaaaacaaa tcagcaaatc
      961 gtattggaaa acatatttac gtatttcaat ttgatctttc ttatcttagc cattttgctt
     1021 tgtttagtag aatcctataa gaacttgact tttttaccag ttatcatagc caatacagga
     1081 atcgccattt ttcaggaaat tcgatccaaa aagatcttgg acgaactaaa tgttttaaat
     1141 acagcaaaag tcaatgtcat acgagacggg atagaacaaa caatagatat agaagacctt
     1201 gtaatagatg acattgttat tttagagact ggatatcaaa ttccagcaga tgcagaggtc
     1261 gtggaaggga aactgcaagt taatgaggct ttattgacag gcgaagcaga tgaaataacc
     1321 aaagaaatag gtgatccatt aatgtccggc agtttcattg tttccggaaa agcttacgcc
     1381 cgattagaca aagttggagc agattcgtat atttcccagc tgacagtcaa agccaagaca
     1441 atgggagaag gagaacagtc ggagatgatt gcttctctta atcaattgat caaatggatt
     1501 gggatcatca ttattcctat cggtatcctg cttttttctc aaagttactt ttttaatgga
     1561 aacacgatca aagaaagtgt cgttgcaatg gaagcagcat tgatcggtat gatacctgaa
     1621 ggactgtact tgctgacgac gattgcatta gcattaagtg cgactaaact agcaaagcaa
     1681 agagttcttt tacacaatat gaaaagcatt gagacattgg caagggtaaa tgtcctttgt
     1741 gtagataaaa caggaacgat cacagaaaat aaaatgtctg ttcaaaaaat gatcgtctct
     1801 aaaaaacaag aaataatcag tcagtcagat caattggaag catgcattgc tgactatgca
     1861 aaagcgatgg caggagacaa tgcaacaatg gaagctgtta aagcatattt tacaaaaacc
     1921 actgatcaat cctatcacca tgttattcct ttttcttctg tccaaaaatt cagcagtgta
     1981 gcgttggcag acaaggtgta tgttttagga gcgcccgaaa tggttttgcg cgatcaattg
     2041 ccggaatatg cggaagagtt tgtttctttc gctgaaaaag gctatcgcgt gttggtcttt
     2101 ggtgtgtacg atgggattct tgaagaacca gtattgacag aagcagtcac accattaggc
     2161 tatattctga ttgctaatcc tatcagaaaa gaagcaagag ccacatttga ttacttcaaa
     2221 aaacagcaag tagccatcaa agtgatttct ggagataatc cgctcaccgt atccaatgta
     2281 gctgtccaag cagggatttc acatgctgat cagtacgttg atgcatctaa attatcagaa
     2341 gaagaatatg aagcagctgt cgaaaaatat acagtttttg gacgcgtcaa gccagaacaa
     2401 aagaaaatct tcgttcaatt gataaagcgt aacaatacag tagctatgac aggtgatggc
     2461 gtgaatgata ttctagccat gaaagaagca gactgcagta tagcgatggc ttccggaaat
     2521 gaagccgcca tgcaggcctc acaagtggtc ttacttgatt ctgacttttc acgcatgcca
     2581 gaagttgttg cagaaggacg gcgcgttgtg aacaatattg agcgatcagc cagcttgttt
     2641 ttagtgaaga atattttctc ctttttgctc tcactgtttt cagtgatctt tgctctgacc
     2701 tatcctttag aaccatcaca aatcacattg attagtttgt ttactatcgg acttccatct
     2761 ttcttgcttg ctctagaaga aaacaaaaag cgaatcagag ggaaattcat catgaatgtg
     2821 atggaaaaag ctgtgccagg tggactaaca gatatgatcg tagtcggtgc gttggtgatc
     2881 tgcggggtga ctttgaatct aaataaaaca gatgtttcta cagcatctac catgcttttg
     2941 attgcagtag ggttcctcgt tctctataaa atctgttcac cgctgaataa attcagaagt
     3001 cagatcattc ttttctgtgc tagcggtaac tttttttcgg tcatcttttt gcataagctg
     3061 ttttcaatca ccggtatctc agctgtatca cttttgctgt tgagcattct atttttctcg
     3121 gcagattcga tttttaggca tttgaccaca ttggtagaat atttgtttga acgtaaaaat
     3181 gaaaaagagc aaaagccgtt tagtcgcgta gctaaaaaat ttctatcgat atttaaacga
     3241 gaaaattgac gaaaagtatc tcaaaaaaga gtaaaaaaga acaaccactc gtgtatcttt
     3301 cgaagcagga tgagtcatcc aacttta
//
LOCUS       DANKKG010000193         3290 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000193 DANKKG010000000
VERSION     DANKKG010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3290
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.195"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(<1..2838)
                     /gene="brxC"
                     /locus_tag="PW004_002661"
     CDS             complement(<1..2838)
                     /gene="brxC"
                     /locus_tag="PW004_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HDL2727586.1"
                     /translation="MLIKDIFAKPIDRNIQGVIKVGQAKDENVQQELEEYVVTKELQK
                     HFKTIFDAYQRSINTPTDKMGVWIQGFFGSGKSHFLKIISYLLANREINDKKAIDYFK
                     EDGKIKDPLTLATMERCSQINTDVILFNIDSKSDAGGKKDKNGIVNVFLKVFNEMQGL
                     STIPHLADFERRLIKEEKFDAFKAAFEEINGAKWQEERHEFDWIQDDIKEAALKIDFM
                     SEAAIDNFLEKASGNYLISIDDFANLVKSYLDSKEADHHIAFLVDEVGQYIGGNRELT
                     LQLQTVVEDLGRICEGKAWVLVTSQEAIDHVTNFDNNSDNDFSKIKDRFATTVSLSSA
                     NADEVIKERILKKKEFANDSLALQFETVQTELKNKLLFVDSPELKLFQDAENYAEVYP
                     FIPYQFNLLGQILTEIRNHSYQGSNLSSGERSLLAMFKEAAERNKENDTTSLVSLDQF
                     YYSLERWIDTAISRVITQATNNSHIQRPTDIDNFNVNVLRILFMIKYVDQAIKPNVEN
                     ITSLMIRNINEDRLETKKRVEEALDLLIKENFVRKNLDSYIFQTDEEQEITREIKNQN
                     ITDSDLIVELSKRIFDGVLDRNSFAYEKLGMSGGNKFKNRYTFTYAQVLDQRPYKQAG
                     NAEMTVNFISPYSDLAGQEAELALKSSRNLLFVELPSDKEFKDDIAMYARMNLYLTTT
                     YSSVVKNFDAIRSEKSGELQELSASINDQLNELLKSAKFFYNGAEIQLTGSDFKAKFQ
                     SALQKMAEEVYHKLNQIDAPKEKEDILYLVKESKNEIIEDPSNEQAVQEIFNFINDQK
                     RNHLTITMKTIRDRFETKQPYGFTELDVEWCIAKLFMSGKIDLLYNNVQISRMDDPNS
                     IVMIIQKRRESEKIKIEPSAVISPKAKKLVDEIGRELFQSRNIVDENNNEHTMENFKK
                     YLKGQLDNLRSYFDLQNTYTYPSAYEYEQIKK"
     gene            complement(2863..>3290)
                     /locus_tag="PW004_002662"
     CDS             complement(2863..>3290)
                     /locus_tag="PW004_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HDL2727587.1"
                     /translation="NDISGTSVGDNYEVKVYNLYVIMLEYLEKRGFTEKAIKMEERRG
                     LQKLVESIGRLLKMDLPEEENVFFEYVDAHTPEKSVVFLTGIGELYPLIRAHSILNKM
                     HQVFDRVPVVMMYPGEYDELSLTILNTNKDDNYYRAFRI"
BASE COUNT          968 a          623 c          468 g         1231 t
ORIGIN      
        1 tttttttatt tgctcatatt cataagcact agggtaggta taagtgtttt gtaaatcaaa
       61 ataagagcgt aaattgtcta attgaccttt taaatatttc ttgaaatttt ccattgtatg
      121 ttcattgtta ttttcatcaa caatattacg actttgaaac agttctctac cgatttcgtc
      181 aaccaatttt tttgcttttg gcgaaatcac cgcgcttggt tcaattttta tcttttcact
      241 ttctcgtcgt ttttgaataa tcattacaat actatttgga tcatccatcc gtgaaatttg
      301 tacattgtta taaagtaaat caattttacc agacatgaaa agtttagcga tgcaccattc
      361 cacatcaagt tctgtgaatc catacggttg ttttgtttca aaacgatcac gaattgtttt
      421 catcgtaatc gttaggtgat ttctcttttg atcattgata aaattgaaaa tttcctgaac
      481 tgcttgctca tttgatggat cttcaataat ttcatttttg ctttctttca ctaaatataa
      541 gatatcttct ttttctttag gcgcatcaat ttggtttaat ttatggtata cttcttcagc
      601 cattttttga agtgctgatt ggaattttgc tttaaagtcg gagcctgtca gttgaatttc
      661 tgcaccgtta tagaaaaatt ttgcagattt caacagttca tttaattgat cgttgatact
      721 tgcacttagt tcctgtaatt caccagattt ttccgaacga atcgcgtcaa agttctttac
      781 tacactcgaa taagtcgttg ttaaataaag attcattcta gcatacattg ctatatcatc
      841 tttaaattct ttatctgatg gcaattctac gaataataag ttacgggaac tcttaagcgc
      901 aagttctgcc tcttgacctg ctaaatcaga gtatggtgaa ataaaattaa ctgtcatttc
      961 agcatttcca gcttgcttgt atggacgttg atctaacact tgtgcataag taaacgtata
     1021 ccggttttta aatttatttc cacctgacat gcctaatttt tcataggcaa aactatttcg
     1081 gtctagaacg ccgtcaaaaa ttcgtttaga tagttctacg atcaaatcac tatctgtaat
     1141 attttgattt ttaatctcac gagtaatttc ctgttcttca tcagtttgga aaatataact
     1201 atctaagttc ttccgcacaa agttttcctt aattaaaagg tccaatgctt cttctactcg
     1261 ttttttagtt tccaggcggt cttcgttgat atttcttatc atcaagctcg taatattttc
     1321 aacatttggt ttgatggctt gatcaacata tttaatcatg aataaaattc gtaagacatt
     1381 tacgttaaag ttatctatat cagttggtct ttgaatatga gaattatttg tagcttgtgt
     1441 aatcactcgg ctaattgctg tatcaatcca acgttctaac gagtaataaa actgatctaa
     1501 agaaaccaaa ctggttgtat cattttcctt atttcgttca gcggcttctt taaacattgc
     1561 tagtaatgaa cgttcaccag acgataagtt tgatccttga tagctgtgat tacgaatctc
     1621 ggttagaatt tgccccaata aattgaattg atagggaata aatggataaa cttctgcgta
     1681 attttcagca tcttggaaga gctttaattc aggactatca acaaagagta atttattttt
     1741 caattctgtt tgaacagttt caaattgtaa ggctaaagaa tcattggcaa actctttttt
     1801 cttcaaaata cgttctttaa ttacttcatc ggcatttgct gaagataaac tcacagttgt
     1861 ggcaaaacga tccttgatct tagaaaaatc attatcagaa ttgttatcaa agtttgttac
     1921 gtgatcaatc gcttcctgac ttgtcactaa tacccaagcc ttaccttcac aaattcgacc
     1981 caaatcttcc acaacggttt gtaactgcaa agtcaactca cgattaccgc cgatatattg
     2041 tccgacctca tcgactagaa aggcaatatg atggtctgct tctttggaat caagatagct
     2101 tttaactaga tttgcaaagt catcaatgct tattaaataa tttcctgatg ctttttctaa
     2161 aaaattatca atagcggctt cactcataaa atcgatttta agtgccgctt ctttgatatc
     2221 atcttggatc caatcaaact catggcgctc ttcttgccat tttgccccat taatctcttc
     2281 aaaagcagct ttgaatgcat caaacttttc ttctttaatt aaccgacgtt caaaatctgc
     2341 tagatgtgga atcgtgctta atccttgcat ttcgttaaat acctttaaaa aaacattcac
     2401 gattccgttt ttgtcttttt taccacctgc atcacttttt gaatcaatat taaacagaat
     2461 aacatctgta tttatttgtg agcaccgttc catcgttgcc aaagtcagtg gatctttgat
     2521 tttcccatct tctttgaagt aatcaatggc tttcttgtca tttatttctc gatttgcgag
     2581 caagtaagaa ataattttta agaaatgaga tttacctgaa ccaaagaacc cctgaatcca
     2641 aacacccatt ttatctgttg gtgtattgat actccgttgg taggcatcaa aaattgtttt
     2701 aaagtgtttt tgtagttctt tcgtaaccac gtattcttct aattcttgtt gaacattttc
     2761 atcctttgct tgaccgacct taataactcc ttgaatattt cggtcaattg gttttgcaaa
     2821 aatatcttta attagcattt aatctcctac cttccatccc aactaaattc taaatgctcg
     2881 ataataatta tcatctttat tggtattaag aattgtcaaa cttaattcat catattcccc
     2941 tggatacatc atcacaactg gtacgcgatc aaatacctga tgcattttat ttaaaataga
     3001 gtgcgctcta attaatggat ataattcgcc aattcctgtc aggaacacaa ctgatttttc
     3061 tggtgtatgg gcatccacat attcaaaaaa tacattttct tcctcaggta aatccatttt
     3121 caataaacga ccaatgcttt ccactaattt ttgtaaacca cgtcgttctt ccatcttaat
     3181 cgctttttca gtgaaaccgc gtttttctag atactctaac ataatcacat agagattata
     3241 aactttcact tcataattat caccaacaga tgttccagat atatcattaa
//
LOCUS       DANKKG010000194         3262 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000194 DANKKG010000000
VERSION     DANKKG010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.196"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(64..594)
                     /locus_tag="PW004_002663"
     CDS             complement(64..594)
                     /locus_tag="PW004_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HDL2727588.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(693..1043)
                     /locus_tag="PW004_002664"
     CDS             complement(693..1043)
                     /locus_tag="PW004_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HDL2727589.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(1200..2729)
                     /gene="ahpF"
                     /locus_tag="PW004_002665"
     CDS             complement(1200..2729)
                     /gene="ahpF"
                     /locus_tag="PW004_002665"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HDL2727590.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(2739..>3262)
                     /gene="ahpC"
                     /locus_tag="PW004_002666"
     CDS             complement(2739..>3262)
                     /gene="ahpC"
                     /locus_tag="PW004_002666"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HDL2727591.1"
                     /translation="AYHQGEFLEVSEKDLMGNWSILCFYPADFTFVCPTELEDLQEQY
                     STLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSHQISRIFDVLDEEQG
                     LAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRTHPGEVCPAKWKESG
                     ETLKPSFDLVGKI"
BASE COUNT         1024 a          668 c          548 g         1022 t
ORIGIN      
        1 caatccaaaa gatttttttg gattgttggt ttttatacgg tctattttaa taactaaaaa
       61 tgattaacgt atagtcattt ttcttattct gatgaaattg ataatctcct ggatgcccat
      121 gaaaatcaaa ataaagccga atatttggaa tagaagcatc aaacttttga atggattgaa
      181 caataagacg aatccgccaa tcaacaacag aatactacta taaacactcc aacttttagc
      241 ttttgtattc atgctgaaga acaactggaa caagctgttg atgataacag ctaatccaag
      301 aagaatcggc aggatagaaa cgattgcagg tgcaaaagca tagacgatca gagctaagat
      361 caggaaaaaa agaccactga acagaccgat actccaagaa cctgttttcc ttttaaaacg
      421 gtaagcctca aaaagattca acaacccaag caaaacaaga taggcggtga tcagataacc
      481 gataaaatga aaaacagcag aagggtttag cacaatcgca atcccagaaa taatataaac
      541 agcagctctt aataaggcgt atttttgaat attttgttta atcgtctcaa acatataaga
      601 aaacctcctt tgacttattt taacgtgaac tagtcatttt accaaattaa atgttacaaa
      661 atgagcgaag gagaagaata tctctggaaa aatcaacttc tgaggatctt ttccattttt
      721 ttgccttttg ctagctcatc gatcatttta tctaaataac gaatttcttt catcaagtta
      781 tcctcgatgt cttctactcg tacaccacaa acaacacctg taatcaagga acgtgaagga
      841 ttggggacag gggcttccgc aaaaaattgt tcgaaagtca cttgtttttg tagttgatat
      901 tctagttctg tttgggaata acccgtcagc caacaaatga tttcatctac ttcctctttt
      961 atacgatctt ttctctccgc ttttgctaca tacataggat aaacgcttgc aaaacttgtc
     1021 gtaaaaatac gattttgcgc cataatcagt cacctttctt tttttcaaat tatttactac
     1081 ttatagtata taagaaaaag agaagaaaaa gcgattattc aaaaaaacaa aaaggattgt
     1141 gacaaaattc ttgtcaccat ccttttttcc gctctctgac tttttacaat cttcatagct
     1201 caagtgcgca tcagataatc gaatgctcct aaagctgcag tagctcctga acccattgaa
     1261 acaatgattt gtttgtatgg actatctgta caatcaccag ctgcgaaaac gccggggata
     1321 ttcgttgcgc catttttctc ggtcactatt tcatttcttg gattcatttc gatcgttcca
     1381 tctagccaat ccgtattagg gagtaagccg actagaatga atactcctga aacagctagt
     1441 gtatgcatct ccatcgtacg gcgatcgata taggagagag attctacctg attcgtaccg
     1501 ctgatttctt tcgtcgctgc atgtttgata acagtgacat tcgcaagaga gactagttta
     1561 tcctgtaaga tttgatctgc ttttaattct ggtaagaatt ctagaacata tacatgtttt
     1621 gctaacccag ccaaatcgat tgctgcttca acaccagaat taccaccacc gataacagca
     1681 atctcttttc ctttaaataa agggccgtca cagtgtgggc aataagcaat tcctttattt
     1741 ttaaactctt tttcgcctgg tacattgata gaacgccagt gcgcacctgt cgcgatgatg
     1801 gcggtttttg ctttaagtga tataccgtta gctaagccaa tattaataaa atgttgctct
     1861 ttatttaagg aaacggcctg ttgattcatc ataatgtcga tagggtaact acgcacatga
     1921 ctttctactt gctgcatcaa ttttggacct tctgtgtaag aggtaccaat catattttca
     1981 atccctagtg tttcaacgac ttgtccgccg aaagaatcag cgacaagtcc tgtacggatt
     2041 cctttacgtg ctgcatagat agctgcacta ctagctgctg gtccaccgcc aatcactaat
     2101 acatcataag gttctttttc aaacaactct tcttcaatca aagggccagt gattttttca
     2161 acaatttgtt cgatcgtcat gcgtccgcta ctaaattctt ctccatttaa aaacacagtt
     2221 ggcactgcca tgattttttt atcatcgatt tctttttgga acatcccacc ttcaatcatc
     2281 gtatgagtaa tatttggatt taatacagct aatatattca atgcctgtac aacgtcgggg
     2341 caattatgac aagtcaagct aacataactg tcaaaatgca atgtttgtgt tatttttttg
     2401 attttaagac tcaaagattc actgattttc ggtgcccgtc cactaatttg taacatagcc
     2461 agtaaaaaag aggaaaattc atgaccgagg gggataccag caaaaacgat tccactttct
     2521 ttgccaactg gattgataga aaaactaggt gtgcgcatcg aatctctttt ttcccaagag
     2581 attttaggag acatttctgc tatttccttc gtaaattcag ctagtttttg actattttca
     2641 ctattatcca aactaacaga aaaaacaata ggagattcaa tcagtgctaa atattgttcg
     2701 agattttgtt tagtttcttt gtctaacata tccaccacct aaatctttcc aacaagatca
     2761 aaacttggtt tcaatgtttc gccactttct ttccattttg ccggacaaac ttcacctggg
     2821 tgggtacgta catattgagc agcgcgaatc ttatcaatca gtgcactagc gtcacgaccg
     2881 attccatctg cattgatttc catagcttga acgattccgt ctggatcaat aataaatgtt
     2941 cctcgctgag ctaatccttg ctcttcatcc aaaacatcaa aaatacgaga aatctgatga
     3001 gacggatcac cgatcatcac gtattcgatt ttaccgatag catctgatgt atcatgccat
     3061 gctttgtgag taaagtgggt atcggtagag caagaaaaga cttcaacacc taaagatttc
     3121 aatgtactgt attgttcttg taagtcttct aattcagtag gacaaacaaa agtaaaatca
     3181 gctggataga agcaaagaat actccagttc cccataagat ctttttcact gacttctaaa
     3241 aattctcctt gatgataagc at
//
LOCUS       DANKKG010000195         3221 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000195 DANKKG010000000
VERSION     DANKKG010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.197"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            301..585
                     /locus_tag="PW004_002667"
     CDS             301..585
                     /locus_tag="PW004_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727592.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="PW004_002668"
     CDS             588..1004
                     /locus_tag="PW004_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727593.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="PW004_002669"
     CDS             complement(1141..1860)
                     /locus_tag="PW004_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDL2727594.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2535..2630
                     /locus_tag="PW004_002670"
     CDS             2535..2630
                     /locus_tag="PW004_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HDL2727595.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2932..3177)
                     /locus_tag="PW004_002671"
     CDS             complement(2932..3177)
                     /locus_tag="PW004_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727596.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1074 a          599 c          503 g         1045 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtactc
     2041 agataaaaag ttgttagtac ccttctgtat ccgttgggag agtaaggctc aactgccccc
     2101 taaaaagggt tctaaaaaat ctttaaaaaa taaataaaat aaacgcgcgc gcgcgagcaa
     2161 aatttcccgc caaaaaatca cccacttttt aaaataaaca ggttcgcgca tgatattaaa
     2221 ctgccaatct agctccctat ggtcgctgta agaaggagtg atcgctacgc tctcacattt
     2281 tacttgcagc agtcgccttt aggctccttc gccaaaaata attcattagg gactcctttg
     2341 tggcgtcctg agagctcgtc taagccgttc taagacaaga ttcgataaaa actatgtcct
     2401 tcgttctaaa gcgccttaca ggcggtcagg gcaagcccta acaaccccaa ggcaataaaa
     2461 aacttaggct tatttttgct tttttattgt ataattgatt gtcgcctatc cgacagtctt
     2521 gcgactggaa ggaggtgtgt ttcgtgttca ctaactttat ttgctccctt cttgttgggc
     2581 ttttaagcgc tttgttcagt tactggttga ataaccgaaa caagaagtaa aacttaatta
     2641 aggcgactca gcccacaaaa aaagccccag agaatccgac ctctctgggg ctttcgtgtg
     2701 tttcgtgttc acttatttgc aatcaaatta taacagaaat aaatatttat ttcaaaaata
     2761 tcctacaaaa gtctaaaatc cgtaattatt tcttatatgc aacatttaca aataaagtcg
     2821 ctctgagagc tcgtattttt cgatttaaga cgttttattt agtttttggt gtatttcatt
     2881 ggtccaagct aaaaaactct tagaagcgat tttagagcca tttaaaccca cttaattata
     2941 ttctccatga aattcgctcc atgcgtcaaa agctgcgtct gaaattccgc cgtcttcgtt
     3001 cactagttgc attaattttt ccaacgatcg ttttctttcg ggtgtcatat ttttatattc
     3061 caacaatttt tcgtctgtta aatcgaactt gattgcttga attgcttgct ctaatttttc
     3121 taagttttga aaatattcgt aaggaaattt ttccattttt tctttctcca gtctcacaat
     3181 ttttctagaa ataaaatcac ttctttgccg tctatactct c
//
LOCUS       DANKKG010000196         3066 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000196 DANKKG010000000
VERSION     DANKKG010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.198"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            5..694
                     /locus_tag="PW004_002672"
     CDS             5..694
                     /locus_tag="PW004_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDL2727597.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            708..2210
                     /locus_tag="PW004_002673"
     CDS             708..2210
                     /locus_tag="PW004_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2727598.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            2223..2699
                     /locus_tag="PW004_002674"
     CDS             2223..2699
                     /locus_tag="PW004_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2727599.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(2796..2906)
                     /locus_tag="PW004_002675"
                     /pseudo
     CDS             complement(2796..2906)
                     /locus_tag="PW004_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1008 a          413 c          603 g         1042 t
ORIGIN      
        1 gataatggat aaaaagaggt ttttagatct tatttcaaaa ggtttgattg tttcctgtca
       61 agcattacct ggtgaaccat tttacaaaga ggaagaaagt ttaatgcctt attttgcttt
      121 ggctgctcaa agggctggag cagttggtat acgtgcaaat tctgttcgag atattattga
      181 aattagaaag tgtgtatcat taccaatgat tggaattatt aaaagagatt atccaccaga
      241 atctccattc attactgcca ctatgagaga agtagatgaa ttagtagaaa ccggtgttga
      301 ggtaatcgct cttgattgca ctcttagaaa tcgacatgat ggactatcta taaattcttt
      361 tattactaaa ataaaagaaa aatatcctca tcagttattt atggcagata tttctacatt
      421 tgaagaaggc aaaaatgcat ataaagcagg aatagatttt gtaggaacta ctttaagtgg
      481 atatactgag gaaagtaaaa aacaagatgg gccagatata gatttgatta aaaaattggt
      541 agaagaaggg atttgtgtaa ttgctgaggg aaggattcat actccagaac aagcaaaaga
      601 gataaaaaaa ataggagttg caggtattgt tgtgggtggt gctattactc gaccacagga
      661 aattgcatca agatttgtta aagcattaag ttagggggag aaataaaatg tttaaaaaat
      721 tttcacaatt aggacgcgct ttcatgttac caattgctat attaccagta gccggattac
      781 tattgggact gggaggagcg ttaacaaatg agagtgctat aaatgcttat ccatttttag
      841 atcaaccgtg gttgcataca attttaagta ttatgagtta tgctggaaat gcagtttttg
      901 caaacctagc actaattttt gcaattggta ttgctgtagg acttgcaaat ggagataaag
      961 gaacagctgg tttagctggt ggagtagctt atctagtata tactgcaact attagtgggt
     1021 ttcttgaact tttttcagca aaagatgcaa cactagatac aggtgtagtg ggatcaattg
     1081 ttgttggtgg tactgttgca tttttacaca atcgttatag aaaaattgaa ttaccacaat
     1141 tcttaggttt ttttggagga tcaagattta ttccgattgt ttctagttta gctgcaatca
     1201 ttattggttc tttcttttat ttaatttggc caccaattca aaatggttta gtagttgctg
     1261 gtgaacaaat tgcccaaatg ggtagtttag gaactttttt atatggtttt ttattgcgtt
     1321 taacaggtgc tgttggacta caccacacca tttacccttt gttctggtat acatctttag
     1381 gtggtaccga aactgtagct ggatcaacaa ttgctggagc ccaaaatatt ttctttgccc
     1441 aactagctga tccaaaccac actggtctgt ttacttatgg tactagattt tttgcaggaa
     1501 gatttgcaac aatgatgttt ggattaccag cagcatgtta tgcaatgtat cgtgcaattc
     1561 ctaagaaaaa tcgtaaaaag aatggtgggc tttattttag cggggccctt acatcatttt
     1621 taactggaat tactgaacca gttgaatata tgtttttatt tgttgctcct tggctttatg
     1681 tgattcatgc attcttagat ggattatctt tttattttgc tgatatctta aatattcgtg
     1741 tagggaattc cttttcaggt ggtttgatcg actttttatt attcggtgta cttcaaggga
     1801 atgacaaaac taattggtta aaattaattc cttttggtat tgcatgggca tttatatatt
     1861 tctttgtttt tacattcttt atcaaaaaat ttaaggtagc tattcctgga atggaagaag
     1921 aatcagaagt tcattctgaa aaagtaataa acaaaaatac atcattacat gaagagtctc
     1981 ttatcattat tgaagctctt ggtggaaaag aaaatattga gtctgttgaa gcttgtgcaa
     2041 ctcgtttgcg tgttgctgtt acgaatgggg agattattca aaaacaaaca ataaaagaca
     2101 ctggtgcaac tgctgtcttc gaagtgaagg gtggcatcca agctgttttt ggagggaaag
     2161 ctgacttatt aagtcaagaa atcaatcaaa tattaggaaa agataattag gaggttttta
     2221 atatgtttga aattttcaaa aagaaaaaac aagaaacttt ttatgcacct tgttccggaa
     2281 aaatcatttc catcacagaa gtaaatgatc ctgtcttttc acaaaaatta ctaggtgatg
     2341 gttttgccgt catacctgat ggttcaaaaa tttatgctcc attggctggt cgagtaacaa
     2401 atatatttcc tactaaacat gctattaata taaaaagcaa ctcaggcatt gattatttga
     2461 ttcatattgg aattgataca gttgaattac aaggagagcc tttttcgatt tgtgttcaag
     2521 aaaatgatga ggttactcct gaaacctcgt tggttgatgt ggattttgac aaaataataa
     2581 aatctggtaa agatacttct gttatagttg tttttccgga gaaaaatcag ttcaatgatt
     2641 taataataga taatggtcga attgctcatg ggaattcatg tggaaaaatc actttttaaa
     2701 aaagtgtata taaaaagcta taaaataagc taaataggat atttgtcaaa tgaagttggt
     2761 gaacagaaag aactgttttc tttctgttca tttttttatt ttattagtaa tgattcccca
     2821 tgccaaatca tgatccgtaa tttaaagtta taaaagcttc gaaatccatt ggcatttcgt
     2881 tggaatacct tgatatgatt attcaaatat aaaatataga gagaagagag agagggaaga
     2941 tatttataaa gggcaaagtt atgtttactc aacttaggaa atagaaatag atcaatttat
     3001 attctcttgt caatcgaata tgaaccttag cactatatac caaatatagt aaaatgctga
     3061 aagatg
//
LOCUS       DANKKG010000197         3024 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000197 DANKKG010000000
VERSION     DANKKG010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.199"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(222..1274)
                     /locus_tag="PW004_002676"
     CDS             complement(222..1274)
                     /locus_tag="PW004_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2727600.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(1287..2225)
                     /locus_tag="PW004_002677"
     CDS             complement(1287..2225)
                     /locus_tag="PW004_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDL2727601.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            complement(2676..2990)
                     /locus_tag="PW004_002678"
     CDS             complement(2676..2990)
                     /locus_tag="PW004_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727602.1"
                     /translation="MYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENII
                     LRTEYGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSH
                     RQ"
BASE COUNT          993 a          636 c          442 g          953 t
ORIGIN      
        1 caaatttttt ctcattttaa cacatatttt tattctagtt gccttttgaa caatcaataa
       61 cataaaggtg agtttcttct acattttaaa aaatcgaatg gcactgaaga gttcagttgt
      121 cagcatcaat ctcctactca aaaaggcaaa aaaacgtaag tcagatgttg ttgacatctc
      181 acttacgcat ccattgattg aaaatttctt agtaaaatct actagcctaa aaatttgaag
      241 acttcttctc gatcatacag tttaaatact ttctcgcata aaaattgagt aattagtgcc
      301 gcaaaaacag ggatgactaa ccaacaaaga acaacaagaa agccatttag ccctgcatct
      361 aaagaagcca acggaccaac aagtccgacc acgccgaaac cagcagaagc aggcgtacct
      421 gaaatagaaa atagagcgac aggaatagct gtaatcactg cactaactaa acaaggaata
      481 agaatcacag gttttctaaa tagattaggc atcatcatct tcatagcgcc taaagcaatc
      541 gcaattgtca cacctgactt attaaccttc caagagttca ctactaaaac aaaagtggtt
      601 gcagctacgc ccatcgcagc cgcaccagca gaaacaccat ctagttggat tgccattccg
      661 attgctactg ttgaaatagg agagataatc aaaaaggcaa aggaacaacc aatcaagata
      721 gacattaaca gcggttgtaa ggtagtgaag ttattaatca atgatccaat tgcagaagta
      781 attgctgtta caaaagggaa taaataaaaa ccaagtaaac ctgcacctac acctacaaca
      841 atcggtgtta acacaatcga cactgaccca aaactatttc caatcagtaa aatcagcccg
      901 acagcaatcg atgctgtcag catcgtatta ataatatcgc ctgtaccagc accaacgtaa
      961 acacccgttt cacccaaatc aggtgtgaat ttgactacac cagaaccaac aaaagaagct
     1021 ccggctacaa ccatcattct catcggatca aatccaaatt gtaaggcaat caaaccacca
     1081 atgatcaaag gtgttgctag ttgaaaaata actgctaact gtgcgatcgt taccgcaaag
     1141 ggatacgcac taaaatattt taaaatggca cctaatactg cattaggaat caatccaata
     1201 atgattcctg ttgctgtacc agcgaggact ttattcagaa aaattttcgg tgatattttt
     1261 tgtgaagttt ccattcaatc tctcctttat tttacgttca aactatcttc ttttgcatgg
     1321 acaagctcag tcaagaaatc acaataaggc gtcgcaacac catatttctt cccttttcta
     1381 gaaattgcgc cattgatata atcgatttct gttaatcgat ggttcttaat caagtcttga
     1441 tacattgaag gataatgtaa cccaattgtt tccgggtcaa aacaagattc acaatgtgca
     1501 atgacttctg ggacatctag ttcaatcttc tctactgctg ctactttggc aaactcattg
     1561 acaatcgtcg ccaccatttt atgagcagtc gatgtttttc ctagctcagc catattaacg
     1621 tctaagattg tgcatagtcc gttcattgtt ccgttcacac atgctttgcg ataaatagaa
     1681 tagtgaatat tgtcagaaaa atgagcattt aatcctgatt cagacaattt gtctgctaat
     1741 ttttttgcag ctgcttcttt tccatcacct aaattttgta attctactga accacttcca
     1801 aataatttga cctgaccagg accttcaaga cctgccgtcc acatggtatt tccaatatag
     1861 atattttcca ttggtacaaa tttctcaata atatcttcat gcccgatacc atttaataga
     1921 cataagacct ctgtatcttt tttgattaat gattggatat cctgcagcat tttttccagc
     1981 tgcatcgctt tggtaaatag aataatcaga tcaacttgat cttctttttc tacttcggat
     2041 tgaagaacga ttggtagttt tgcttctacc tctttaccat taaaatttgc ttgtaatcca
     2101 tgctctttga tttgttggac atgttctgcc cagccatcga ttaataatac ttcgttccca
     2161 ctttggtgaa gcattaaccc aaagcgactt cccattgcgc cagcgcctgc aattgctatt
     2221 ttcattgatt cttcctcctt tttctttcta ttatattcat ttgagaatca tttcacaacc
     2281 ttgttttgtt attcttctct tatttatcat gtttatatcc accattcaag ttattttatc
     2341 agcaaatcaa taaaaactgg aatagaatcc caatttcatc gtaagagaaa ctagactagc
     2401 aataagaaaa tagaaacatt catagaaaaa acctcactcc tctgattata aaagaggggg
     2461 cattaacctc gtgtatacta aaaccttcca ttcattcgat ggatgatttt ctttttgtga
     2521 aaaaatattc attgtagtac gatgttgcgc aacacagacg gatggtattg cttattattt
     2581 attaaaatcg aacaatacac ttatttaaaa acttctttcc cagattttct tccattttta
     2641 cagttataac tagtattatc atgaaaaata gccttttatt gtctatggga ataataattt
     2701 aaaattggta cttttttatt aattagttca tcgcccaata atctctccag tttcttagca
     2761 tctcctgact caaccggaat tatccaagcc cagtccaaat aattttttaa aatctcatca
     2821 tattttgcaa atatttcatt gccatattcc gtccttaaaa ttatattttc agtgccagac
     2881 aagtgatttg tattgagttg ataaaaaaga tctccatcta aaaaaatatc attagaagaa
     2941 gatttcccca cagtaatgac cattccatca gctctatgtg ttacatacaa ataattgtat
     3001 gacctcattt cacctaatga atca
//
LOCUS       DANKKG010000198         3017 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000198 DANKKG010000000
VERSION     DANKKG010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.200"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..799
                     /locus_tag="PW004_002679"
     CDS             <1..799
                     /locus_tag="PW004_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727603.1"
                     /translation="TYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLV
                     PWYLLQNTPAKLLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLI
                     TLTVSSKKNFNQKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKH
                     EAKIGSQWVNLPYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNAN
                     GLVQISGVKGVADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            974..1807
                     /gene="msrA"
                     /locus_tag="PW004_002680"
     CDS             974..1807
                     /gene="msrA"
                     /locus_tag="PW004_002680"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDL2727604.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQGERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            1920..2876
                     /locus_tag="PW004_002681"
     CDS             1920..2876
                     /locus_tag="PW004_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL2727605.1"
                     /translation="MEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQREILRGAFDMG
                     ITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMWEGPYGEWGSRK
                     SIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGKALYIGISNYNG
                     EDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGAITFSPLAQGLL
                     TNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQSLAQMALAWNLR
                     QKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
BASE COUNT         1093 a          478 c          572 g          874 t
ORIGIN      
        1 tacttactca atgggactag ttggtctgtt atctttatgt attcccctga ttttatttaa
       61 aaaattaaca aacatagaaa aaaagattta ttatggtacg tgtttgactt ggctagctac
      121 aactagtttg gtgccatggt atcttctgca aaatacgcca gccaaattgt tacaatttcc
      181 ttggcggatc ttaagcttgc aaataatatt ctcttctctg atattaacga tgatattttt
      241 caaaaataga agatacaata agacaagaga actattttat ttatttatga ctgttttgtt
      301 attaatcact ttaactgttt ctagtaaaaa aaatttcaat caaaaaataa tttctcagcc
      361 gggacatatt gttgtaaaca aagaaacagt agaggcgtat accactaaag gaatggatta
      421 ttttttatat gattattctc ctatcgcttc attggagaat aaggagcata taataaaaca
      481 cgaagcaaag attggcagtc aatgggtaaa ccttccttat aaaacaactg gtagttctat
      541 catatataga gtaactaatg agaagagcca agtaatcaca cttcctgtat atgcttattt
      601 aggaacgaat gtagaaataa atcatcaacc tgtaaaggag caggtgaatg ctaatggatt
      661 ggtacaaata agcggagtca aaggagttgc tgatattaaa gtaagcacaa gttatacagt
      721 aacgactatt atttcttatc ttgttacatt aactgcatat ttatgtttat tttttaaaat
      781 agttttccat aaaaggtaaa ttaacttttc atttataaaa taaattgatt aaaaaggaga
      841 atctgtcgat aaggattctc tcttttttat acgaaaaatc tgtaggggga tgaagtaatc
      901 gcttatttcc tcactttatg ataaattaca gctaaagtca ctagcttgtc ttcttcggaa
      961 aggaggagaa tctatggatg aggaacaaat attcattcaa acactttata atctgatttt
     1021 gaatccgaat acacgagatt gggaacgaaa agtattgatt cagacaaaga atgacataag
     1081 agaaaatata tccgttaaag aacagttatc aaaactagaa gcaacactac gtccattagc
     1141 tatccgaatg aatctgacgc ctgatgtaat ggacttttat ttattgatta cggaaggatt
     1201 tgataaggag caaaaatatg acttttccaa gcatgccatg caagatgctg actatcaaga
     1261 acgtgcagta tttgcagggg gatgtttctg gtgtatggtc gagccgtttg aatcgaaaaa
     1321 agggatccta tctgttttgt caggatacac gggcggacat gttgaaaaac caaattatga
     1381 tcaagtaagt ggtggttaca caggtcatgt ggaagctgtg gagatcattt atgacacaag
     1441 agaaataagt tatagcgagt tgctgacaat ctattggcaa attactgatc ccacagatac
     1501 atttggacaa tttcaagata gagggaagca atatcgtcca gtgatttttt atcaaggcga
     1561 acgacaaaaa gaattagcgg aacaatcaaa acagaaacta gacagttcag gaactttcca
     1621 ccaaccaatc gtcacaaaaa tcgaaccagc cggtacgttt tggccagcag aaaactatca
     1681 tcagcaattt tacaaaaaac aaccgaaacg ttataaaaaa atccagcaag cacgtaatca
     1741 atttctgatc tatcaacgag taaaaaacaa atggcaaaaa aatatacgaa aaaatcattt
     1801 tgattaactc cttttcttta aaatgtagta aactaaaaat agaaaaaaga aagcgtttta
     1861 aaaagatttg atgaggagga gaaaaattgt atattgcaga tagtgcacgt tatgaaaaaa
     1921 tggaatatcg taaatccggt cattcaggtt tgaaattacc gattttatct ctcggattat
     1981 ggcaaaattt tggtgattat gatcctatcc ataatcaaag agagatttta agaggcgcat
     2041 tcgacatggg aattacccat ttcgaccttg cgaataatta tggaggccct gctggagctg
     2101 cagagaaaaa ctttgggcgt atttttagag aagattttca agcctaccgt gatgaactga
     2161 ttatttctag caaagcaggt tatcatatgt gggaagggcc atatggggaa tggggctctc
     2221 gtaaatccat catttcaagt tgtgaccaaa gtctccaaag aatggggcta gattatgttg
     2281 acatcttcta tcatcatcgc ccagaccctg atacaccttt ggaagaaaca gcagaagctt
     2341 tgatgcaact tgtaagacaa ggaaaagcat tatatattgg tatatccaac tataatggag
     2401 aagatacaaa aaaaatgact gaaattttaa aaagaaagga agcaccgttc attattcatc
     2461 agatgcgcta caatatgttt tctcgtgcac tactagaaga cgatctatct ccagtattag
     2521 aagaagaagg gctaggggca atcacgttca gtccgcttgc tcaagggttg ctgacaaatc
     2581 gttatctaca tggtattcct gaagattcta gagcacatcg taaggaaata ccattccttt
     2641 cagaagaaca agttggatca actctggaaa aaatcaaagc actgcaaaca atcgccgtat
     2701 cacgaggaca atcattagcg caaatggccc ttgcatggaa tttgcgacaa aaatctgtta
     2761 caagtgtact ggtcggagca agtcggttaa gtcagttaca agaaagtgtt cgaatgatgg
     2821 ataatttaga tttctcacca gaagaagaat tgagaatcga ccaaattcta gaatagaaac
     2881 gatgtaatca aactttttat ccatatatta aaaaaaacaa aagatgacat caagaatcga
     2941 atgaagatcg tctgttattg aacatcatat tttttgtcgt tcaaaataaa aatgatcgaa
     3001 ggctaggagt gaatttt
//
LOCUS       DANKKG010000199         3011 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000199 DANKKG010000000
VERSION     DANKKG010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.201"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            69..1370
                     /locus_tag="PW004_002682"
     CDS             69..1370
                     /locus_tag="PW004_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2727606.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            1373..2224
                     /locus_tag="PW004_002683"
     CDS             1373..2224
                     /locus_tag="PW004_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2727607.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            2237..>3011
                     /locus_tag="PW004_002684"
     CDS             2237..>3011
                     /locus_tag="PW004_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HDL2727608.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLW"
BASE COUNT          820 a          575 c          581 g         1035 t
ORIGIN      
        1 tatcttgctc ttgttgccat tatcttaaaa gcagtttgcg gtttagcaag tttagagggg
       61 gcattcagat gcagcaagat aaaacaagca atatcaacga aaaaaaaatc acgctttgtt
      121 cacttattcc agggatgggg cagtttgtaa atggccaagc ttttaaaggt atcgtttttt
      181 tcgccatatt cgtcctattc attggtcaga tgcttctggg cggatttcat gcattggtag
      241 gattggttac tttgggaagt ataccaatgg aagatcattc attatttatt ttgattgaag
      301 gcactttgca attcatcatt acaatcattt tcttgatttt ttacggtttg aatcttttag
      361 acgcttatcg agtagcgaag atgcgtcaag agggcaaaaa aatcaatcgg acgatgaagg
      421 aagtcataca taatataggt gatgaaggtt ttccttatct attgacactt cccgcttatc
      481 ttctcatgat ttttacgatc attttcccag tcctagttac attgttcact gcatttacta
      541 attatgactt caatcatatt ccaccagcca gtttaattga ttgggtaggg gctaaaaact
      601 tttttagtat cttcttttta agctcatatc gtaatacttt ttttgcagtc ttttcgtgga
      661 ccgtgatttg gactttatgt gcaacaactt tgcaaatcac ccttggtatt ctcacagcgg
      721 ttgttgctca ccagaaattt attaaattca aacgtatttt cggtatcatt ttcttattac
      781 catgggcggt cccagctttt attactatca tgagtttttc gaatatattc aacgatagcg
      841 taggtgccat caatactcaa gtgattcctt tcataaacca gcttcctttt gtagatatca
      901 gtgcaatctc ttggaaaacg gacccgaact ggacgaaagt agcaattatc ttagtgcagg
      961 gctggttagg atttccctat atctatgtga tggttacagg aatcttacaa tcgatttcag
     1021 aagacttata tgaagcagca aaaatcgacg gagcaaatgc atggcagcgc ttttcaaaca
     1081 tcacgcttcc tgctgtcttc ctagtggcag cgccaacatt tattacacag tatacgggga
     1141 atttcaataa tttctccatg atctatctgt ttaacaatgg tggtcccgga agcgtaggcg
     1201 gaggagctgg ttctactgat attcttattt cctggatcta taaacttacg actggaactt
     1261 ctccgcagta ttctatggct tcagcaatca cgttgattat ctccgtcatc gttatctgtg
     1321 tgtcattgct ggtatttaaa aagacaaatg cgttcaatat gggggattaa caatgaaaaa
     1381 atttcattcg agtgctcgat tccaaaaacg actttctcac ttttttactt acttttttct
     1441 tttaatactg tcagtcatta tcatctatcc gcttttgatc acagcaagta ctgccttcaa
     1501 gtcaggaaat attgcagctt ttagtttaga tctttcaagt gactggactt taggaaactt
     1561 tactcggcta ttcagtgaaa cactttacgg gacttggtat aaaaatacat tgatcatttc
     1621 tgtcctaaca atgatcgtac aagtgactgt agtaactttg gcgggctata cttatagtcg
     1681 ctatcgtttt atcggccgca aatcaagttt gatgttcttt ttagtgattc agatggtacc
     1741 gactatggca gcattgactg ccttttatgt catggctttc ttattagaag cgctcgataa
     1801 gtactggttc ttgacaatga tctatatagg cggtggaatt ccaatgaata cttggctgat
     1861 gaaaggatac tttgacactg taccaataga tttagatgaa tctgctaaac ttgatggggc
     1921 agggcatttc aggatttttt tccagattgt attaccgctt gtcaaaccga tgctcgccgt
     1981 tcaagcatta tgggctttta tgggaccttt tggggatttc atgttagcta agttcctgct
     2041 tcgaacacct gaaaatcaga ctgttgctgt cggcttacaa acatttattt ctgatgtgac
     2101 aaaccaaaga gtctctcttt ttgctgctgg ggcaattttg attgcactac cgatttgtct
     2161 attattcttc tttttacaaa agaactttgt taccggcctg acatctggcg gaacaaaagg
     2221 ataggagcag agtatcatgc aaacatttcc gttaaactat ttttccgcat tacgtacgcc
     2281 aactcaagtg tttgcgggac gcaaactatt atcatggcct aaatttttct tgatatttgt
     2341 atttttagtt tccttgatgg tcatgcctgt gactcttttt tatgccaatc agatccaagc
     2401 gattccatta gaacaatttc tatccgttca ttcgctgatc gatgaacaag gaacccaaaa
     2461 attttctgaa cttgaattat ctgaaactgg gttacaagca tcgcaacaaa cgatagctgt
     2521 gacccctgaa attttggtag gtgtgtcttt atctgagaaa caacaatccg atcatggaac
     2581 ttttattgat tttgagaagg aacaatgggt gatccaacaa aaggataaat ctggtatacg
     2641 ccgctatacg atgaattata gcccatcttt ccaaccagat tctgtacgta cgcccgaaga
     2701 tttccaacga tttttagaaa gagaatttta tgccagtaat cgtccaacaa tcattttatc
     2761 ttatagttta agtcttggtc ttgtcctatt tgtcatgacg agtctcattt tattcggggc
     2821 ttcctttttc ttgtggatga caagaaaaag ccaactttca tccattcata cattcaaaga
     2881 gagtgcaaat ttgatgctga atgttatggg aataggttcc atggcagcag cagttgtagg
     2941 gtttatccac tttgacttta tcttgatgct aagtgtccaa acaacgataa ctgtcttgct
     3001 gcttttatgg g
//
LOCUS       DANKKG010000200         2994 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000200 DANKKG010000000
VERSION     DANKKG010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.202"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            56..655
                     /locus_tag="PW004_002685"
     CDS             56..655
                     /locus_tag="PW004_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2727609.1"
                     /translation="MKSVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLRDVLEISRGRVIVKERLAMKEQKTAKHNSVFISNKLRKVILDYVQSEFPEQ
                     LQAQDFSKYLFPSRKGADTPLTRQSLWRIIHEAGTAVGLKEIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            704..1420
                     /locus_tag="PW004_002686"
     CDS             704..1420
                     /locus_tag="PW004_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HDL2727610.1"
                     /translation="MNEEKQPNFPDKYHLSRKESVYLLKKNIVELVYNAGKFEGLNTT
                     LLQTEGIIKYNRANNVAVDDVLTVVNLKRGFEMLLNDVQEPLLETSKRINRIVAAEDA
                     LFPGEIRTGGVELSTIQGRYVPPMLTEDEVNNQYGEIMNQEISDTEKALRLFLFISKN
                     QIFWNGNVGTALLIANKIMFSNGLGLLSIPENVFMKFNELLSKYCNSNLLVNESNIIS
                     FLYEQCIFGINYLKIIQHTI"
     gene            1434..2213
                     /locus_tag="PW004_002687"
     CDS             1434..2213
                     /locus_tag="PW004_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002396205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HDL2727611.1"
                     /translation="MMTIDEAKQLATYFSVGSAGYILDFTNVTWNIFTKKIVGIEIQN
                     HYGGSKGVSFQKFLLDDQFDDSLKNRLIQSLKGIMDRDKKYESTEMNPIVQLLFEEVF
                     KEDTSTALRKMKFSSEIDLLYIRQLPIRIQTDIKNKDFDSVLTKANTLIDEVLKFIIE
                     NTEGPSVNINLFKSKELRKEVFGRLNIKTEKDMDNRIKALVGALNTLSDKILEMRNSQ
                     GDAHAHGSNRIVIKEEEAILVANSSMVLCEYVFTKYKGLNR"
     gene            2296..2652
                     /locus_tag="PW004_002688"
     CDS             2296..2652
                     /locus_tag="PW004_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL2727612.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVDNGRYVITHGFTKKTEKTPITE
                     LKHAKELREEWFTNDD"
     gene            2642..2908
                     /locus_tag="PW004_002689"
     CDS             2642..2908
                     /locus_tag="PW004_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2727613.1"
                     /translation="MMIKKLIEEKKKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
BASE COUNT         1108 a          433 c          607 g          846 t
ORIGIN      
        1 tgtgacttat tatctgttat aatcgtatta agtaacattt gagaggtgtc taaaaatgaa
       61 aagcgtggaa ccgattcgcg acaaaaaaaa gatcgatgcc atgaaggcca ttttggcttc
      121 cggaaaatat gggcaacgaa atttggtgct tttttcgatc gggatcaata cagcctatcg
      181 aatctccgat ttaagacagt taaaattaag ggatgtgcta gaaatttccc gtgggcgggt
      241 gatcgtcaaa gaacgtctgg ccatgaagga acaaaaaacg gccaaacaca attcggtctt
      301 catttcgaac aaattgcgaa aagtgattct tgattatgtt caatccgaat ttcctgagca
      361 gttgcaagca caagacttca gcaagtattt gtttccaagt cgaaaaggag cagatacgcc
      421 tttgacccga caaagccttt ggcggatcat tcatgaagca ggaactgcag ttgggttaaa
      481 agaaattggt ccccattcga tgcgtaagac ctttggctat tttttataca agcaaggaac
      541 caaaacagag atcattcagt cgctgctaaa ccattcttca caacgggaga ccttacgcta
      601 tattggaatc acccaggaag acaaagatac tgcagtaaag agtttggatt tatgacagaa
      661 taatgcccta tataatagaa ggaaaaattc aagaggtgag aaaatgaacg aagaaaagca
      721 gcctaacttt cctgataaat atcacttatc tcgcaaagag tctgtgtatt tattgaagaa
      781 aaacattgta gaactggtgt ataatgcggg gaaatttgaa gggttaaata caacgttgtt
      841 acaaacagaa gggattatta agtataatcg ggccaacaac gtggctgtag atgatgtcct
      901 gaccgtcgtt aacttaaaaa gaggctttga aatgctttta aatgacgtac aggagcccct
      961 gttagagaca agtaagcgga ttaatcggat cgttgcagca gaggatgcgc tttttcccgg
     1021 tgaaatacga accggtgggg tagaattatc gacgattcaa ggaagatatg tgccaccaat
     1081 gttaactgaa gacgaggtaa acaatcaata tggtgagata atgaatcaag agatatcaga
     1141 tacggaaaaa gccctgcgac tgtttctatt catttcgaaa aatcaaatat tctggaatgg
     1201 taatgtgggt acagccttgt tgattgcgaa taagattatg tttagcaacg gtttaggact
     1261 attatcaata cccgaaaatg tatttatgaa atttaacgaa cttttatcaa agtattgcaa
     1321 cagtaatcta ttagtcaatg agtcaaatat tatatcattt ttatatgaac agtgtatctt
     1381 tggtattaat tatttgaaaa taatccaaca tacaatttaa gaggtataag taaatgatga
     1441 ctatagacga agcaaaacaa ttagcaactt atttttcagt tggttcagcg ggatatattt
     1501 tagactttac taatgtaacg tggaacatat ttacaaagaa aatcgtaggg attgaaatac
     1561 aaaatcatta tgggggctca aagggagtat cctttcaaaa gtttctgctt gacgatcaat
     1621 ttgatgattc gttaaagaat cggttaatcc aatctttaaa aggtattatg gatcgagata
     1681 aaaaatatga atctactgaa atgaatccca tagttcagtt gctttttgag gaagtattta
     1741 aagaagatac ttcaactgca ttaagaaaaa tgaaattttc aagcgagatt gatttacttt
     1801 atattcggca gctacccatt aggattcaaa cagatattaa aaacaaggat tttgactctg
     1861 ttttgacaaa agcaaatact ttaatagatg aagttctaaa atttattatt gaaaataccg
     1921 aaggaccgag tgttaatatt aatttattta agtcaaaaga attaagaaaa gaagtatttg
     1981 gccgcctcaa tattaaaact gaaaaagata tggataatag aatcaaagcc cttgttggag
     2041 cactcaatac attatcagat aaaatactcg aaatgagaaa tagtcaagga gatgctcatg
     2101 cacatggctc aaatagaata gtaattaagg aagaagaagc aattcttgtt gcaaattctt
     2161 ctatggtttt gtgcgagtat gtgtttacta agtataaagg attaaataga taataaaatg
     2221 catattttat agtatacaat tcgaattttt atgttatata ctataatata aaatcaatat
     2281 atgatataat tgggtatgga aaaaatatct tttgaaacat ttaaaagacc caatggtcat
     2341 gatgaatttt tagaatggtt ggaaacatta ccaaaaaaag atagtgcgaa gcttttacgt
     2401 acaatagaag aaacagaaaa gaatgggatg ctagtagctc aacggttgaa atgggtgaaa
     2461 aaattagaca ctaatttgta cgaattaaga agtaaggtag gatcaaacat ccaaagagcc
     2521 atctatttcc atgtggacaa tggaaggtat gtcattactc acggatttac taaaaaaacg
     2581 gagaaaacac caataactga actcaagcat gcaaaagaac ttagagagga gtggttcaca
     2641 aatgatgatt aaaaaattga ttgaagaaaa gaaaaaagat aaagattttg cagaagcata
     2701 tgaccgtgaa ggaagaaaac tcgaaactgc ggttgctctt tatcatgctc gggaggaagc
     2761 agggctaact caagcggagc ttgcacaaaa agcacataca actcaatcta ctattgctcg
     2821 aattgagaga ggggataacg tatctttcga taaacttgat gatatcgcca gggcattagg
     2881 taaaaagctg gtagtagaat ttgcttaaaa gcgagttgaa aatatatcta taataggctc
     2941 ctaactaatc aattagttta gtgggccttt ttttactaga tagataaagg ttgt
//
LOCUS       DANKKG010000201         2978 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000201 DANKKG010000000
VERSION     DANKKG010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.203"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(63..614)
                     /locus_tag="PW004_002690"
     CDS             complement(63..614)
                     /locus_tag="PW004_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HDL2727614.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFLRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            complement(788..1087)
                     /locus_tag="PW004_002691"
     CDS             complement(788..1087)
                     /locus_tag="PW004_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HDL2727615.1"
                     /translation="MLETAIEVLEKCAQLVTASEEWGYESVTMEKEEIEMGMLPEDVH
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTDE"
     gene            complement(1114..1380)
                     /locus_tag="PW004_002692"
     CDS             complement(1114..1380)
                     /locus_tag="PW004_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HDL2727616.1"
                     /translation="MDSLPVELQALCWNLIDQNVQKQFPLDYLQIFEFSTEKGNQKLV
                     HRQEEPEERKEYLISPKLRLKSVSQKIWAIDSGEYQTMLLPEEY"
     gene            1727..2410
                     /locus_tag="PW004_002693"
     CDS             1727..2410
                     /locus_tag="PW004_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727617.1"
                     /translation="MTTEHFDLANPVTKVDDIPDYEMYSQTIDSLNKRFGNRVLKGIK
                     IGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDDEVKDMDPAIVIPQYFAQLSE
                     ALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQFLPILDQVIKNKLAFELNAKSAYLY
                     DNLALYEYVIDLYLSRGGTLFSVGSDGHYLEHFRFHFDDLFALLKANGVTELAIYQKG
                     KRIMVPLPV"
     gene            complement(2447..2944)
                     /locus_tag="PW004_002694"
     CDS             complement(2447..2944)
                     /locus_tag="PW004_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2727618.1"
                     /translation="MNNQITLAYLDYDDLDALQVVSVVSFYESFIDGADPLDMQGYLQ
                     TQLTTEILAVELAQSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRLYLLKDYQNKGLG
                     QRLLDEANRYAKTKQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPI
                     LEKEI"
BASE COUNT          903 a          561 c          455 g         1059 t
ORIGIN      
        1 atgtattttt aaagtcaaaa agcatgtaaa actacttata agtaacatga gaaaataaaa
       61 tgttattttt cttgaaataa taattgacta tctgccaatt gccattcatt acgaacatga
      121 atacttagtg ggtgggataa cttaccttct gcatttatca atactttggt catcttcttt
      181 tgtttcgcac tcaattgatc gtaaatagtc tttgcttgtt cataagccac ttgactcgtc
      241 gctgtaatcg aaccacaagc aaaaataatg gtattgattt tcttttctgt aaaaacagtc
      301 gttaatattt ccaaagattg ttcatcattt cctcgaagaa atacccgagg acttttcgct
      361 tttgtgtacg aaaaaagatt cacagcaata aaaccagtaa atcctaattg tcggatattc
      421 ttttcgatta ataacatact caaatcattc gtaaatggtt ctgtatctgt tggacgaata
      481 gtaatgacta ctgctaattt atcttccgct tcttttcctt tgatcgccca ttgtcgtttg
      541 aaaacatagc gatgggtatg atgaggatct cgccaaactt ctgtttgtgt ggtcactaat
      601 tctttttcaa tcatctcttt ttctcctatg ctattttttg ttcaagcact tcaattacca
      661 ttgccgttat aagtgtcaat actagttgaa aatatttcat attctttctc cttttttctt
      721 cagaatttga tgaatcgcgg taaggatatc ttctaaacat ttcgcaatca ttctcaaata
      781 atcgatctta ttcatccgtt cctccaatct gactccaaac caatcgtcct tctaccagta
      841 acccacgaac aaattctcgc tggctggtaa tatctgtaat gaaataaact acatagtctt
      901 ttccatcttc tggtgcgcaa taaatataaa gatgtgtcat cactaaacgt ggtagatgaa
      961 catcttcagg aagcataccc atttcaattt cctctttttc catcgtaacg gattcatagc
     1021 cccactcttc agaagcagtg accagctgtg cacacttttc cagtacttcg atagccgttt
     1081 ctaacaagtc ctttcgttcc tttcttttag atactagtat tcttctggta ataacatcgt
     1141 ttgatactca cccgaatcaa tggcccatat tttttgagac actgatttca aacgaagctt
     1201 aggtgaaatc agatactctt ttctttcttc cggttcttct tgacgatgaa ccaacttttg
     1261 atttcctttt tccgtagaaa attcaaaaat ttgaagatag tctaaaggaa attgcttttg
     1321 aacgttttga tcgattagat tccaacataa agcttgtaat tccaccggca aactgtccat
     1381 tactcccttt gtgacataac gttatccttt attttgaaac atttcttttc cctcctttgc
     1441 cataaaaata gcccaatctt ctattaaaga agattgggct tcatggatat aatatatatt
     1501 tgtaaataaa gttaattcag ttataattat caaaactaca caattattaa agtttttcat
     1561 atctctttgt ttagtaagtt tttctaaatc ttaacccgtg gtgctagtta attttttcct
     1621 agaggaaaat gtctaaacat gttggtaaat caacaaaaaa acgcatatag tagtctcaaa
     1681 actcccaaga aatgaggact atttaacaaa ctacgatggt tacgtcgtga caactgaaca
     1741 ttttgactta gccaatcctg ttactaaagt cgatgatatc cccgattatg aaatgtacag
     1801 tcaaacaatc gatagcttaa ataagcgttt tggcaatcgt gttttaaaag gcatcaaaat
     1861 cggttacatc gcttctgaaa aggatcggat catcgactat ttagccgata aagattacga
     1921 tttaaaatta ctcagtgtgc atcataatgg gcaatttgat tatttagatg acgaagtcaa
     1981 agatatggat cctgctattg tgattcctca gtattttgca caactttcag aagcactagt
     2041 tgttatagaa gcagatgtct ttgctcattt tgactatggt tttcgggtat ttggtctatc
     2101 cgttgctgaa tttaaacaat atgaagcgca gtttttaccg attctagatc aagtaatcaa
     2161 aaataaattg gcattcgaat taaatgctaa gagcgcttac ttgtatgaca atttagcgtt
     2221 atatgaatat gtcatcgatt tatatctttc acgtggtggt accttgttta gtgttggatc
     2281 agatgggcat taccttgaac atttccgttt ccatttcgat gatctttttg ctctgttaaa
     2341 agcaaatggt gtgacggaat tagcgattta tcaaaaagga aagcgaatta tggttccatt
     2401 accagtctaa aaaaacgtcc tcagcatcaa aagtgaggac gtttttttag atttcttttt
     2461 ctaaaatcgg acaaatacga tcttgttttc ctaaaggaaa atgtttgatc ccgatttttt
     2521 taaatccatg acgttcataa aaacgaatac ctgcatgatt tttttcataa acagtcaaac
     2581 gtaaataacg tttttgtttc gttttagcat aacgattcgc ttcatcgagt aaccgttgcc
     2641 ctaacccttt gttttgataa tctttgagca gatagagccg ttgaatctct atagcatctt
     2701 tttcatcatt gactttcata taaccaatga gtatttgatg atccttaata ccaataaatt
     2761 tactggttga ctgtgccaat tctactgcca atatttcagt tgtcaattgt gtctgtaaat
     2821 atccttgcat atcaagtgga tctgcgccat cgataaacga ttcataaaaa ctgacaacac
     2881 ttacaacttg taaagcatct agatcatcat aatctaaata cgctagtgtg atttgattgt
     2941 tcatctaact cctccttttg acatagtata ctataaat
//
LOCUS       DANKKG010000202         2968 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000202 DANKKG010000000
VERSION     DANKKG010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.204"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            273..539
                     /locus_tag="PW004_002695"
     CDS             273..539
                     /locus_tag="PW004_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HDL2727619.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPSYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            536..967
                     /gene="ruvX"
                     /locus_tag="PW004_002696"
     CDS             536..967
                     /gene="ruvX"
                     /locus_tag="PW004_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HDL2727620.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            992..1297
                     /locus_tag="PW004_002697"
     CDS             992..1297
                     /locus_tag="PW004_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HDL2727621.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(1378..2523)
                     /locus_tag="PW004_002698"
     CDS             complement(1378..2523)
                     /locus_tag="PW004_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2727622.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(2584..>2968)
                     /locus_tag="PW004_002699"
     CDS             complement(2584..>2968)
                     /locus_tag="PW004_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDL2727623.1"
                     /translation="HMMPEIKRNVLEKQLKKTIEKNNSIIDSNLSNAINNIEEDNLTV
                     SQKIYLTNAIKYLKTGSRIEFPDGSTPIAYLAALLNTLLRYDSLINDSEKNTDEKKEY
                     QEFYENDLLKEFTALVTELNKHYSK"
BASE COUNT          916 a          484 c          588 g          980 t
ORIGIN      
        1 acaaaaacaa aaaattagta gcagctattc gaatttttta tgaatattat gaaaaaattg
       61 tgtaaaaaca tacattcaaa gaaatatagc gtatacttta tgaaaagagt acttttgata
      121 aagctacaga aaaaaggaaa aaaacataca aatagtgttt cttaatttat tcggaagttt
      181 ggaatatttg tttgtagttt tccctttctt cgagtaaaat aaggttgtag aaggtaagct
      241 ggaattacct tgcgggaaag aggtgtagaa tcatgggttt tacagatgaa accgtacgtt
      301 ttggttttga cgatagtcgc aaaaaagaag tgagcgaaac attagcgatt gtttatcggg
      361 ctttggaaga aaaaggctac aatccaatca atcagattgt cggctacttg ctttctggag
      421 acccgtccta cattcctcgt tatcgtgatg ctcgaaattt gattcgccgt cacgaacgcg
      481 atgagatcat ggaagtaatt gtcaaggact acctctcaaa tcatggagtg aatttatgag
      541 agtaatgggc ttggatgtcg gttctaagac agtaggtgtt gctatcagtg acccctttgg
      601 ctggacagct caaggagtag agatcattcg gatcaatgaa gcagaagaag agtttggctt
      661 tgatcggttg aaggaattag tagcacaata tgaagtagac cggtttgtag ttggattacc
      721 taaaaatatg aacaattcca tcggaccaag ggcagaagct tccatgtcat acggagaaaa
      781 gatcaaagaa ctattctcct tgccagttga ttatcaagat gaacgattaa ccactgttca
      841 ggcagaacgt atgctagtag agcaggcaaa tacttcaaga gccaaacgaa aaaaagtcat
      901 cgataaatta gcagctgtga tgatcttaca aaattattta gacggctcag gtaagcaatt
      961 attttaataa taaaagaaaa gaggtaatct tatgacagaa cataaccatt cccacgacca
     1021 tgaaggacac gaacacatca cattggtgga tgaccaagga aatgaaacat tatatgaaat
     1081 cctactaaca gttgacggac aagaagaatt tggacgtaac tatgttcttt tatatcctgc
     1141 tggtgtttca gaagatgaag atgtagaatt acaagcttat gcatacatcg aaaacgaaga
     1201 tggaacagaa ggcgagttag aacaaatcga aacagacgca gaatgggaca tgattgaaga
     1261 agtattcaat acattcatgg cggaagaaga agaatagact ataaagctag gagaaacctt
     1321 gatatgatag ggtttctcct ttttttattt tttactgtat gccatattgt atgccatcta
     1381 gtcaatattt aagtgttgta ctaatttttc aacagtcttt tctttcgttt cttttgccaa
     1441 gtgaccgtaa caatcgatcg tctgactaat ttttgtatgc cgtaatctag tttgaacttc
     1501 taccatagta gctccagcgt ccaagagcat tgaagcatga gtgtgtcttg ctacatgcac
     1561 accgagaaat ggaaccatag ctttttcaca aatagtaatc aatcttgcat aaatagcgtg
     1621 gtgtgtgatt agttctttat agataccaca aaaaatcata gtcggatcag ttacaccaag
     1681 tgtcagcata tatttccgct gattaatctg ccattttttt aatatagaaa ttgttctttc
     1741 atctagtgga agaaccccag cactacaatc agttttagga tcactgattt tatagccatt
     1801 tttggattgg cttagtgttt tattgatggt gaccgtattt tcttgaaaat caatgtctga
     1861 ccaattcaat gcaagggctt cactgatcct aaccccgcta taaaagagaa agcgtagtaa
     1921 cgttttatca aagtcgtttc gataagtttc aggtttgctg tttaggtaat caaacaaggt
     1981 gatcagttgt tctttactgt aaactttgac cttttcttta tgcttagtgg tggttctttt
     2041 aggcattaat acgttgtcta gtggattagt ctcaataatc ccaagattga tagcgtagct
     2101 aaaaatacgc ttagaagttg aatgaagtac cttgtattgg tcagtagttt cagcccagct
     2161 atttacaatt tgttgggctt tttttaccgt gacttcattc acacgaagat ttccatattt
     2221 tggaagaatc cactttctaa acttgatttc agttttcatg aaggtggctt ctcggacagt
     2281 ggttttatat gtttctagcc acaagtcata aacttcttta aacgtttcgt tgttcgcttt
     2341 tttaataccg tttttatcgt agtccacttg caagcgtgac aaggcaagct gggcttcttt
     2401 ttttgttttg taacctcgtc tacgaacctt atcaggtttg ccagtcattt tgttcattcc
     2461 aagataggct tcatagagcc aaaatttacc ttttttagtt tcatattgct tgaatgttgc
     2521 cataatagtt ttccctccat agtgtagcaa gccgtggggg cggttggagg aggcagttta
     2581 ttattatttt gaatagtgtt tatttagctc agtaacaaga gctgtgaact cttttagaag
     2641 atcattttca taaaattctt gatactcttt tttctcatct gtattttttt ctgaatcatt
     2701 aattaatgag tcatatcgta aaagcgtatt tagaagagct gcgagataag caattggggt
     2761 actcccgtca ggaaattcaa ttcgagatcc tgttttaaga tattttattg cgtttgttaa
     2821 ataaatcttc tgtgagacag tcaggttatc ttcttcgatg ttattgatag cattagaaag
     2881 attagaatca attatagagt tatttttttc aatagttttt ttcaattgct tttcaagcac
     2941 atttcgctta atttcgggca tcatatgc
//
LOCUS       DANKKG010000203         2950 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000203 DANKKG010000000
VERSION     DANKKG010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.205"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            2..1393
                     /locus_tag="PW004_002700"
     CDS             2..1393
                     /locus_tag="PW004_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HDL2727624.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            1409..2449
                     /locus_tag="PW004_002701"
     CDS             1409..2449
                     /locus_tag="PW004_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL2727625.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            2468..>2950
                     /locus_tag="PW004_002702"
     CDS             2468..>2950
                     /locus_tag="PW004_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL2727626.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     H"
BASE COUNT         1029 a          455 c          549 g          917 t
ORIGIN      
        1 tatgaataaa aatggagaat ggagtgctgc acgacggata ttcattatat taatggatgt
       61 tattgtatac aatgcagcaa tatatagtag tttcttactg aagtttcgtg gagagatccc
      121 attacgaaat ttagaaactt tccaacactc agcaattatt atttcagtga tttttattgc
      181 gttgaacatt ttgttgggtg catatgtttt ttataatcga atgatcagtg acatcgtgtt
      241 tgtcacagtc atcggtcagg tattgatgac actagggatc atggtagtga cttttgcggg
      301 ccgctggttt gcttttccac gtcttgttat cttactttca tttgttctcg gtacgctttt
      361 cttaagtatc tatcgtattc tcgtatataa actttattta cgcgtaagtc gagataaaaa
      421 ggtcgtcatt attgggttag aaaaggatgt cgcaccagca atccaaaatt ttggttcaaa
      481 gaaaaacaac aaacataaag tcgaagcagt cattcttgat aactattatg caaacatcaa
      541 aaatatgatt aaagacatcg atattgtcta tcttgcttct catatcgaag aaactgaaaa
      601 aatcaaaatc tatcaattag ttactaaaaa tgaaaaaaaa ttgtttttga atacaacatt
      661 tgaaaacttg acattgatca atccaaatat tatgaatttc gaagatgaaa gtattattga
      721 agcatctgga tttagaatcc aacctgaata tgaactattc aaacgtttgt ttgatatcat
      781 cgtttcactt gttttattag ttattgcttc accatttatg ttattcacag caattttagt
      841 caaagtgaca tcaccgggac caatcatcta taaacaagtg cggatcacaa aaaatcaaaa
      901 ggaattttca atctataaat tccgctcgat gaccgccaca gccgaagcaa aatctggacc
      961 agttttagct aagagcaatg atgcacgagt aacgcctgta ggtaaattta ttcgagctgt
     1021 ccgctttgat gaattaccac aaatcttcaa tgtgctgaaa ggcgatatgt cgatcgtagg
     1081 accacgtcct gaacgtcctt tcttcgtcga tcagttcaat gcagaaaacc cttattacta
     1141 cttacggcat aatgttcgtg ctggtatcac tgggtatgca caagtctatg gaaaatatgt
     1201 ctctgattac aacagtaaat tacgttttga tttactttat atcaaaaaat attcattgat
     1261 gcttgattta aagatcttgc tgcaaaccat caagatccta tttgataaaa tgtcctctca
     1321 aggacttgaa gaagacgaaa atgtgatctc attggcagaa attgcttttc cgaatgaaaa
     1381 aatctttcgc tagttgggag gcgtaaaaat gaacaatccg aaattcagca taatcgtacc
     1441 agtatacaat gcagaagcaa ctattggtcg aacgattgat attctaaaag aattggatta
     1501 tgaagagtac gaagtcgttt tagtaaatga tggatcaaca gatcaaacac aggaaatcat
     1561 tcaagaactg attgaaaatg atgaacgttt tgaattgatt actacgacca atcaagggcc
     1621 cggattagca agaaacgaag gaataagaca gtcaaaagga gaatatctct tattcttcga
     1681 tgcagatgac acgccagtaa aaacgatctt gtcagattat gatcatttgc tagaaaagag
     1741 tccaaaagcg gatcttgttg ttagttcgtt tgtttttcgt acaatggacg agggaagaat
     1801 cgtttccgaa aaagaaaact tagttacaga atgtcactat ctaactcacg aagagtttat
     1861 aaaagacatg tatcagttaa tgaatgaaca gttgatgtat gttgtatgga acaaatgcta
     1921 tcgtagcgat attctgaaaa ataaccaaat tctatttaaa acttataata gttgtgagga
     1981 tcgaatattt aacttacatt attatgaata ctgtcatcaa gtgatcatga acccaaaagt
     2041 agaatatatt tatgaatttg aaggtgggaa ggggatcact aatcaatacc gacctaacaa
     2101 gttcgaaaca tttaaagaat tctattcatt agcaaatact gtaacagacg atattaataa
     2161 agacggtatg gcggcgcttc ttttaaaagg gacaacttca gttatttttt ctatttatag
     2221 tacctctgca agaacttcaa aagagaagaa agacgaagca aagcagatat tgagtgatcc
     2281 agtgatccga gaagccaagc aaattgctaa gacggattct tcagcaaaaa aaataacaaa
     2341 gttgctttac aatatgccag ctccactatt tttatctgct gtaaaagttg gaagcttcgt
     2401 tgaagtgaaa atgccagggt taatggctgc tttgaagcga gtatattagt taatggagga
     2461 aaatataatg aaaagaattt tgcattttca gggacgaatg ggtttaggtg gagcagagtc
     2521 tttcatgatg aatttatatc gtaaaattga tcgtacaaac tatcaatttg attttttgat
     2581 ttatgaagat tatgcagatg ttcaagacta tcattcagag attgaaagat taggaggccg
     2641 tatttttgtt gtacctaatc ctaagaaaaa tatttttaaa tatttaatag aagtgaataa
     2701 gttactaaaa aaagaatctt tttctatagt tcacaatcag gtttattttg gcgggggaat
     2761 taatttatgg ttagccaaaa agaatggaat cagacagaga attgctcata gccatgcaac
     2821 tgaagatgga aaatctcaaa atattgtaat gaatgtattg cgtaaatttt taaccaaatt
     2881 gttgttacaa aatgcaacag actaccttgc cgttagtcaa gaagctggtg aaagtctgtt
     2941 tcaaaaccac
//
LOCUS       DANKKG010000204         2856 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000204 DANKKG010000000
VERSION     DANKKG010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.206"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            89..319
                     /locus_tag="PW004_002703"
     CDS             89..319
                     /locus_tag="PW004_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727627.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKNRSE"
     gene            325..624
                     /locus_tag="PW004_002704"
     CDS             325..624
                     /locus_tag="PW004_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727628.1"
                     /translation="MLFNETQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDIVTDVLDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(1139..1212)
                     /locus_tag="PW004_002705"
     tRNA            complement(1139..1212)
                     /locus_tag="PW004_002705"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1176..1178),aa:Ile,seq:cat)
     gene            complement(1401..2420)
                     /locus_tag="PW004_002706"
     CDS             complement(1401..2420)
                     /locus_tag="PW004_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HDL2727629.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RADVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(2417..2641)
                     /locus_tag="PW004_002707"
     CDS             complement(2417..2641)
                     /locus_tag="PW004_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HDL2727630.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            complement(2638..>2856)
                     /locus_tag="PW004_002708"
     CDS             complement(2638..>2856)
                     /locus_tag="PW004_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727631.1"
                     /translation="RPIAASFSKITETLSKVSHNLDLLTKDLESSKSDRLMIHEELKK
                     HDERLDTHTEKLVEHTQQIKTLFRERRK"
BASE COUNT          890 a          520 c          489 g          957 t
ORIGIN      
        1 atcagtcaaa ttatgcccct tttttgcccc tcaaatatcg ctttacaaca agaacgtaag
       61 ttcgtataat cgttttgagg tgatttttat gatggaagaa tttattagaa aaaatatcag
      121 tgatgaatat gcagactttt atgatcaaag cagtaaaaaa gataaattcc agatggatgt
      181 ttcaatttta gctatattag ctttttccga aaataaacaa cctgtaactg caaaaaaaga
      241 aacagtattc tctgaaggca aaataaaaac tcgatatata ttagaggtag agactaagtt
      301 taaaaataga tcggagtaat ggttatgctt tttaatgaga cgcaattatg gtttaaattt
      361 gatccttcga atagatttgt caaagatttt tataaggtgt gggattcaga agttttcttt
      421 ttagcaatcg aagatagctt attaattaat ctctactatt ctaataagaa ctactttaaa
      481 atccctgctg cgaaaactag aatgaagaaa gacgtatatt ttttgtttga tatcgtgact
      541 gatgtgctag acgctcgaag cgatcatcgg cgttatgact atataaagta tactttcgtt
      601 gatccagaaa gatacaaaga ttaaagtagg ctacctaaaa aggtagcccg gaacggattt
      661 tatcaccata cttatgaaag gagatatttt ttaagttagt attaagattg tgtaatatga
      721 tgatatctat attttatagt atcagtgcta taaaatcaaa aataagtcac taattaacta
      781 ccactccaat tataagcctt ttttctcact ttttttcaaa aatatggtat gctttttaat
      841 ggcttcaaat ataaaagagt ttaaagcgta acacacttat ggggcagtgg tttttgggga
      901 acgctttaaa ctcttcttta ttattatctc acaatataac ccaaatgtct ttctatttaa
      961 aaatcaaagt aaaacttttc aaatatacag aaatataact atgtgaaaca tcctttcatt
     1021 aatccataaa aggatacata aaaaagccac tcatttgagt ggcaatgaag aaaagcttta
     1081 gcttgtataa tactcttcaa aaaattctaa cacagaacga ttcaaatggc tacgttaatg
     1141 taccctgtag gactcgaacc tacgaccgga cggttatgag ccgtctgctc tgaccaactg
     1201 agctaagggt actggttgtt gccacataaa gccataaaca atcaaccagt agaatgtgtg
     1261 gcaacaaacc tgttatcgca tagcttggag tgtgactatt atgggtgata gtgaagatat
     1321 gcgataacat cactatttta tcgaatgatt tttatagttg tcaatatagt tatgtaatgc
     1381 tcctcaacga ggagctattt ttatcgttta ggaatattta aataccaacg tttgtcatgg
     1441 aaatcttgcg caccgccttt agtgtttccc tctggatcat tcgttgcccg catcatgaca
     1501 tagactttct tattagggaa gttacgcatg ttaaaagata catgatagcc aacatttcca
     1561 gaagtattat aagcttgatt tacatctgct ctataaattc catcagctct tactcgagct
     1621 aattctttcc cagtattgta atccataatg aagatatact cgtatttata gttagcaatg
     1681 tgccatccag ctacatgcaa atttgcattt tcgatttctc caaactgatc aatgtgggcg
     1741 taattcgttc catctgtcag tgtaggatta gctgcaccag ctcgtgttgg atcaatgaca
     1801 ggcttgtttt cagaagttgt tggattttca tcggtaaatc catgagctaa atcatatgct
     1861 aatttttctt tacttacgcc catttcagaa agataaccgt aaggatctgt atgatcgccc
     1921 cagatgtttt gtgttaccca taaatgcgat ttgattcccg gttggttata aggagtgtcc
     1981 aacgttaatg gaataccata tttcattgct gaatctctag ccaattcaac gtatgccttg
     2041 tagtttttct caaacgttgc tttatcatgt gtgtgttgta actcaatctg cacaggactg
     2101 ttggcattag catacgaacc agcaccgtac tgtacataac caggttgacc gacttgataa
     2161 acaattccgc cgtctcccac aatataagca gtataagcgc tagtccatga acgttgcata
     2221 tactgcgctt cattgcgtcc tgttgctgtt tcattagccg tttcatgcag taaaatatac
     2281 tgattatttg ctacttgtga gctaccttca tttgcgccca aattaaattc attgttgata
     2341 gtataggcaa atccgttaat tggcaataaa aaaagagccg ttaacaggct catcgcagta
     2401 atagtaattt tctttttcat ttgtttcctc cttcttcgct ttcagccgag aacattttgt
     2461 aggttcgatt tgatacaccc aacacactcc ctaaaaacgc gccaaaacca gtaatgatga
     2521 caacacagat atctgtgtac tgccaattga gcgctttacc aactaacccc acgaaagtag
     2581 ctagtgcggg aataattacc agtgcgaacc attttagtac ttcgaacgtt ttattattca
     2641 ttttcttctc tccctaaaca aagttttgat ttgttgcgtg tgttccacca atttttctgt
     2701 atgtgtatct aatctttcat cgtgtttctt tagttcttca tgaatcatca atcgatctga
     2761 tttgctcgat tctaaatctt tagtcagcaa atctaaattg tggcttactt ttgaaagagt
     2821 ctcagtaatt ttcgagaaag atgcagcaat cggtct
//
LOCUS       DANKKG010000205         2807 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000205 DANKKG010000000
VERSION     DANKKG010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.207"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(58..609)
                     /gene="raiA"
                     /locus_tag="PW004_002709"
     CDS             complement(58..609)
                     /gene="raiA"
                     /locus_tag="PW004_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDL2727632.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            complement(737..1456)
                     /locus_tag="PW004_002710"
     CDS             complement(737..1456)
                     /locus_tag="PW004_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HDL2727633.1"
                     /translation="MRCSCCGKDIIQNLTFSEIIGVSKSRLPQTCSVCRAQFHLLEAK
                     GCAGCQKPIHLKYEETKKKANTSFVYCNDCQEWKKKYPAYDFQHRAFFTYDEKMQAWI
                     NRYKFMGDIRLAGTFAYKWTQIKKDYPAYIYCPIPLSEKRWLERGFNQVSEMLKAAKI
                     PFQMLLKREQHLLAQAKNTRKERLEMPQPFELAVPLSKIQHKKILIIDDVYTTGQTLF
                     HAADCLQNAQPKIIRTISLAR"
     gene            complement(1453..2529)
                     /locus_tag="PW004_002711"
     CDS             complement(1453..2529)
                     /locus_tag="PW004_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDL2727634.1"
                     /translation="MFDQPEAKPREVVFFWTGQLTEKQTEIAKRILCHSEKRHHLIWA
                     VTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNELFLRYRYVFPEEKITLLHGNTA
                     TAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYNGDAGLAHAVQTAIRSNGRFIY
                     LSATPDEKLLKEIKQTFELHKLALRFHRRLLPEPVLVFMNNWRQKCGNKKKINSLVRI
                     IQQLIKENRILLFCPSITMMHRLKITLQEAFPEYEITDVSSQDDQRAKKVQKMREAIY
                     EILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQIAGRADRKGPYTNSRVYFFYE
                     EKTSAIRKACQEIKEMNEEGKQML"
BASE COUNT          770 a          593 c          503 g          941 t
ORIGIN      
        1 cttgcggcag ctgtcagtag ctgctgcaag ttttcgttgt tatttaagta aggctgatca
       61 gtctgtttca atcagtccgt attttccatc tttacggcgg taaacgatac ttgtaccatt
      121 tgtttctgca tcttcaaaaa taaagaagtt atgtcccagc atgttcattt gtaagactgc
      181 ttcttcacta tccattggtt tcaatgaaag gcgtttcgta cgtacgatat ctaattcaga
      241 atcattttcg ttttcgtcct cttcattgaa taaagcagca gcatctgcag tattcatgcc
      301 cgtttcacgg gatttacgat taatttttgt tttgaatttg cggatttggc gttccaattt
      361 gtcaacaacc aaatcgatac ttgcgtataa atctggagaa gtttcttcgg cacgtaatac
      421 aagataaggt aatgggatcg ttacttccac ttttgctgtt ttttctgtat aaacttttaa
      481 gttcacatgc gccgttgcat cgggtgcgtc actgaaatag cgttctagtt tgcctacttt
      541 tttctcaacg tagtcgcgaa tcgcttcagt tacttcgata ttttcgccgc gtacattata
      601 tctaaacata aaaattaccc ctttcgtcta ccacaatcaa gaaggagaag gaccactctt
      661 tccctcttct taattctatt atatcgaact ctaggttatt tgcaaattaa atcgctatca
      721 aatttccaca tctgattcat cttgccaaag aaatggttcg gatgattttc ggctgtgcgt
      781 tctgcaagca atctgctgca tgaaaaagcg tctgtcctgt tgtatagaca tcgtcaataa
      841 tcaatatttt tttatgttgt atcttagaaa gaggtacggc aagctcaaaa ggctgaggca
      901 tctccaatct ttcctttcgc gtgttttttg cttgagcaag cagatgttgc tctcttttga
      961 gcagcatctg aaaaggaatt tttgctgctt tcaacatctc gcttacttga ttgaatcctc
     1021 tttctagcca ccttttttca gataaaggta ttggacaata aatatatgcc gggtaatctt
     1081 tttttatctg agtccattta tatgcaaaag tccctgctaa tcggatatct cccataaact
     1141 tgtaccgatt gatccaagcc tgcatctttt catcgtacgt aaaaaaggcg cggtgttgaa
     1201 aatcatatgc aggatatttc ttcttccatt cttgacagtc gttgcagtaa acgaaactag
     1261 tatttgcttt ctttttcgtc tcctcgtatt tcaaatggat tggtttttga caacctgcac
     1321 aaccttttgc ttctaataaa tggaattgtg cgcggcaaac tgaacatgtc tgaggaagtc
     1381 tagatttcga cactccgatg atctcgctga atgttaaatt ttgaatgatg tctttaccgc
     1441 agcaactaca tctcacagca tctgcttccc ttcttcgttc atttctttga tttcctgaca
     1501 ggcttttcga atagctgaag ttttttcttc atagaaaaaa tagactcgac tgttcgtata
     1561 aggaccttta cgatcagctc ttcctgcgat ttgcaccaaa gaggatttag taaaaactgg
     1621 atgatctgct cctaaaacga tgacagagat tctttcaaat gtgactcctc tttctaggat
     1681 cattgtggtt aacagtatct cgtatattgc ttctcgcatt ttctgaactt tctttgcccg
     1741 ttgatcgtct tgtgaagaaa catctgttat ctcatattct ggaaaagctt cttgcagtgt
     1801 gatttttaaa cgatgcatca tcgtaatact tggacaaaat aaaaggatac ggttttcttt
     1861 gattagttgc tgaatgatcc gcaccaaaga gttgattttc tttttgtttc cacatttttg
     1921 tcgccaatta ttcatgaaaa caagcacggg ttcaggaagc aagcgccgat gaaagcgcaa
     1981 ggcaagtttg tggagctcaa atgtctgctt gatttccttt agtaacttct catcaggcgt
     2041 ggcacttaag tagatgaatc ggccgtttga tctgattgct gtttgcacag cgtgagctag
     2101 cccagcatcc ccgttataag gaaacgcatc gatctcatcg atcactatta aatcaaagga
     2161 ccgataaaaa cggtatagct gatgaatcgt acagataaca aaagaagtaa aggtataagc
     2221 cgttgctgta ttcccatgga gcaacgtgat tttttcttct ggaaaaacat aacgatagcg
     2281 taaaaacaat tcattacaaa catcgactcg cggtgtacaa atcgctactc ttcctccagc
     2341 ttttaaagtt tccactaaaa taggataaag catctctgtt ttccctgcac ctgttacagc
     2401 ccaaataaga tgatgtcttt tttctgaatg gcaaagtatt ctctttgcga tttccgtttg
     2461 tttttcagta agctgtcctg tccaaaaaaa gacgacttct cgaggttttg cttcaggctg
     2521 atcaaacaaa tataaatctt gttgagtgtc gcatcaccct aaatatagac aatgtgtaca
     2581 aaaataaccg taatctgcaa gctgtccaac aatgacggac gtcctcattc cacagcgatg
     2641 acaaagaatc cagttgccca aacgttccac tgctgcgatc ttctcataat ttggtaaatt
     2701 tacctcctca cagtctattg ctttaatatc tctcgtcaga agccttctac cccaaaaatg
     2761 attcatatta ttcgccctaa aaaaatatac gaaaaaacaa gcgatct
//
LOCUS       DANKKG010000206         2796 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000206 DANKKG010000000
VERSION     DANKKG010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.208"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="PW004_002712"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="PW004_002712"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HDL2727635.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         1085 a          435 c          569 g          707 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatcag gaacttacct atccatatat caatggtttt taaccaatgc
     2761 tcaatcttta gtcttactag cgattgtggt cattgg
//
LOCUS       DANKKG010000207         2789 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000207 DANKKG010000000
VERSION     DANKKG010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.209"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            233..610
                     /locus_tag="PW004_002713"
     CDS             233..610
                     /locus_tag="PW004_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL2727636.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            598..936
                     /locus_tag="PW004_002714"
     CDS             598..936
                     /locus_tag="PW004_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL2727637.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            1070..1513
                     /locus_tag="PW004_002715"
     CDS             1070..1513
                     /locus_tag="PW004_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HDL2727638.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            1775..2590
                     /locus_tag="PW004_002716"
     CDS             1775..2590
                     /locus_tag="PW004_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2727639.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            2600..>2789
                     /locus_tag="PW004_002717"
     CDS             2600..>2789
                     /locus_tag="PW004_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727640.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGC"
BASE COUNT          921 a          428 c          566 g          874 t
ORIGIN      
        1 cgttgtcatt tttatacccc ttttcctaaa atttccctaa attgttaaaa gtatatccaa
       61 aaaagaataa aaaaacaaga cttttttatt tgaaataata cacaaacaac cgatatagat
      121 aatccagctt agttttaata gaaatgagta ttgacttgga gtacacttaa agttgtagaa
      181 taaataaaat acataagtaa agtggcaaga aattaagaga aagagtgatc agatgaatat
      241 caaacaagtc agtgaagaaa agggcatttc ggccgataca ttgcgctatt atgaacgaat
      301 cgggttgatt ccaccagtta atcggacaaa aggcggaatc cgtgattata cagaagaaga
      361 cctaaaatgg gtcgacttta cgatctgtat gcgaaatgcc ggattatcga tcgagtcttt
      421 gattgaatat attcgcctat ctagcgaagg agaagcaact gtatttgagc ggcgtaagct
      481 tttgattgaa gaaagtgaac ttttgacgaa agaaattgct gaaatgcaag agtgtttgga
      541 acgtttacaa ggaaaaatca agaaatatga tcgtgtgtta tctagaaata aaatggaatg
      601 tatcaagtaa aaaaatcaag gagcatcgct atggagttac gcttactaaa atatttttgg
      661 acaattgcag aagaaggaac aatctctcaa gccgcagaag ttctacatct tacacaacca
      721 accttaagcc gacaactgaa ggagttagaa gaggaactag gaacagaact gtttgtacgc
      781 gaaaaccgca aaatgatctt gacagaagca ggttattttc taaaaaagcc gagcagaaga
      841 aattttagat ttaacgaatc aaaccactca agaattcgag aacagaaaaa aacaattatt
      901 cagcggccga tttacgatcg gctgtgtgga agctgataat tcgaatacat tggcactatt
      961 actagaaaag ttgatccgtg attatcccag cgttcaattt catatccaca gcggaacaag
     1021 tgatgatatc acggagtgac tagaaaaaag cctgttagat ttagcgattt tgctagatcc
     1081 agtagataca gagaaatacg aaacgatttt gttaccaaga aaggaaaaat ggggattgct
     1141 cattttagaa aattctgttt tagcaaaaaa agacgtgata acaaaagaag actttgcgaa
     1201 tgtacctctt ttgatctcaa agcgtccaga aatacagcga atgtttgctg actggttagg
     1261 cataaaaaag gagcagttgc tgatcacagg aacgtacaat ttgattttca atgtatttgc
     1321 gctaattgag aatcaggtag gtgctgcttt agtcatagaa ggtgctgtaa gtcatctttc
     1381 aaagcgagtg acattcgttc cgcttgaccc aatgattgaa acgaactgca tgctagtctg
     1441 gaaaaagaat cggattttat cacctgtagt caatgaattg attcaccgtt tcaaagaagc
     1501 ttttgaacag taataagtat ctctaatcga agaaatttca atacaaaagc gagacgtatg
     1561 acagtcttgc ttttgtattt tttcattttt aaatcaaaaa aaataaagag atttacataa
     1621 cttgttaaat aagcgcttac aaacataaga agaacggaaa tgaatcagct ataataaccg
     1681 ttgttctgga ttgtaatcat tacaaagaaa tgaagattat aactttggag gtgttcgaaa
     1741 tgaaagaaaa aaagcaaaaa ttcgttcaaa aaacatgccc tttattcaat ggttgggtat
     1801 gattctgctg attgtattag ctttattgac gatcattcct tttttatgga tggtttctgc
     1861 ttcattcaaa gcaaataatg aagtgttcac gattccgatt caatggatcc ctaaaacttg
     1921 gcatttagag aattatctgg ttatttggtc tcgtattcct ttagtgacat tttttaagaa
     1981 tacagctttt ttaagcgttg tgatcacgtt gattcagttg tttacttcta gttttgctgc
     2041 ttacgggttt tctaaactaa attttaaagg gcgcgatatt ttatttttag catatatcgg
     2101 cacgattgct gtcccttggc agtcttatat gatcccgcag ttcattatga tgagaaaagt
     2161 gggtttaaca tatacgttgt ggtcgttggt gctattacaa gcgttcaatg cttttggtgt
     2221 gttcttgttg aagcaatact acagtagtat cccagattct ttatgtgaga gtgcgcggat
     2281 cgatggctta tcagagtggg gaatctatcg caagatcatt ttacctctta caaaaccggc
     2341 gattgccagt ttgacgatca tcacattcgt taatacatgg aacgattata tggggccatt
     2401 catttatttg tcttctacgg aaaataaaac aatccagtta gggttgaaaa tgtttattgg
     2461 gctatttgat gcagagtacg cattaatcat ggctgcctca gttgtttctg ttcttcctgt
     2521 tgcttttata tttttagcga tgcaaaaata ttttgtagaa gggatcgctt catcaggaat
     2581 gaaggaatag gaggtgatta tgaaagagca gcgttttgac aaaaatgttt atatggaatt
     2641 cttttattgg ttgtatcaat cattggcaag tagcctttgt ttttggtgtg tcaatatctt
     2701 gtatatagca gctgcgttcc ttttgtcgat agatggaagg aatgtctttt cctttatttt
     2761 ttctttactt tttattggtc caggttgca
//
LOCUS       DANKKG010000208         2711 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000208 DANKKG010000000
VERSION     DANKKG010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.210"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..375
                     /locus_tag="PW004_002718"
     CDS             <1..375
                     /locus_tag="PW004_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727641.1"
                     /translation="EWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIVDYFANTP
                     EEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKKKEYNYIV
                     KAKVSKRFIFNPKLEIYSVVIE"
     gene            700..1317
                     /locus_tag="PW004_002719"
     CDS             700..1317
                     /locus_tag="PW004_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2727642.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            1358..1657
                     /locus_tag="PW004_002720"
     CDS             1358..1657
                     /locus_tag="PW004_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HDL2727643.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            1691..1858
                     /locus_tag="PW004_002721"
     CDS             1691..1858
                     /locus_tag="PW004_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL2727644.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(1903..2508)
                     /locus_tag="PW004_002722"
     CDS             complement(1903..2508)
                     /locus_tag="PW004_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2727645.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT          949 a          409 c          495 g          858 t
ORIGIN      
        1 gaatggaata atctgactca aaataataaa aataattctg gatgtggaat tatctttatg
       61 tttgttatag ttttgggaat agtagtactt ttaataactg tcaagatagt agattacttt
      121 gcgaatactc ctgaagaaca aatacagaga aaacttcctg agtttgaaag agtaccacct
      181 tatgatccaa gagataattc atttcaagta aaaaaattaa aagagtctcc aaatgaaatg
      241 tggtttgaag ttacagttga atcaagcgac ttcaaagaaa aaaagaaaga atacaattat
      301 attgtaaagg caaaagtatc aaaaagattt atttttaacc ctaaattaga aatatatagt
      361 gttgtaatag aataatagtg ttaaaaaaat tgtaaaagtt aaagaagtca aattctgatg
      421 tgaaaaataa aatataccat ggaagacata aaagaaaaac tggataacta gtttatttaa
      481 tggcagaccg ttaaggcctg ccatttttat gtattttagc ggaataaata taatcatgcg
      541 ggagtgtaaa cttactgttt caaaaagtat actttttgac atgccttgat tttctttgtt
      601 tatgtatggc ataataacca cataatacaa tttgaaacgt ttctgattta atctgattgt
      661 tttgaaacaa ttttagtgaa aaaagggtag ataaaaacga tgagaaaatt tttttatatc
      721 cgggtcagta gttatgatca atcgatcgaa cgacaactga tttccgcgaa ggaattagga
      781 atccctgaag aatatatatt tgtagaaaaa gccagcggca aagattttaa acgccctgaa
      841 tatcaattaa tgaaacggat gtttcgaaaa ggagatttac tctatctaca atcattagat
      901 agactaggca gaaataaaca aatgatttta gaagaatggc gagaattaac taaggaaaag
      961 cagattgata tttctgtgtt agacatgcct ctgttagata ctgctagtta caagcaacta
     1021 gatgggttag agacgcttat ctctgactta attctgcaat tactcagcta tatggccgaa
     1081 gacgagcgga aacgaattaa tagccggcaa agagaaggcg tagaagctgc gaaacaaaag
     1141 ggtgtaaaat tcggcagaaa gaaaattgct ttacctcctg aatttcctgc gatctattct
     1201 gactggaaag caggaaagat aactgcagtc tatgctatgg atcaattggg catgaaacga
     1261 aatacctttt atcgacgggt gaaagaatat gaacatacct tgcaccactt ttcttgatag
     1321 atataacagc tattagacgg gaagggagag tttgaaaatg gacttaacga atcaaagggt
     1381 cgtttttact ggaacgctgc agcaaatgac acgaacccaa gccatcggtt tggttcactc
     1441 tttaaacggc cattgtcaat caagtgttac ttcaaaaaca aatttcctgg tttgtggtca
     1501 gtattctaag tcgttgtttg atgattctct ctatacaaaa aaggaacaaa ccgcgcggga
     1561 cttgattgcg ttaggtcatg agattaccat tttatcagaa gtcgaatttt atgcgttaat
     1621 tagtcaacag ttgaaagaat ggcaacggta tttataatcg ttagtaaaat ttacaaggag
     1681 gaaaattttt atgaagcgaa aatcttatga tcgttggggc attgaaataa ctagttttaa
     1741 tcgaaatatt gcttttcacg tggagtgtga ctgtggggaa ttagcaaaaa gtgtttatca
     1801 aaaaaattac ttttactgtc ctaaatgcca tcgccgatac ggggtacatg gaacatagaa
     1861 acgaaatagt gcggtagaaa agcgaatgaa tgtagtatat ttctacaaat cattcgcttt
     1921 ctttatttta taaatagttg gtcgagagat cccgttttct tttgcaatct ctgcaactgg
     1981 aatgccttgc tttaacatgt ccaccacggt tttaaaaact aagcgttttt gcggatctgt
     2041 tgagttaata gaatactcga ttggtcgtcc cttatatcgg ccggcttttt gagccaataa
     2101 tactccttgc cgctgacgtt cacgaatttt ctcacgctcg ttttgtgcaa tataacttag
     2161 cagtgataaa aacatatcga acatagcggt atccaggagt tcatttccag tattaaaatc
     2221 taaaaactgt gcatctaata tttttaacgc tacttttttc tgtttcataa aagcaactgt
     2281 attttttata tcttcataat ctcttcctaa acgatctagt gatgtaacga tgacgcaatc
     2341 accttcacgg atataattta gtaatttttg aaactcagga cgctcagtat ctttaccact
     2401 ggctttttct tggaataatt tatcaatgtt aagtccttcc atcattgcca attgtcgatc
     2461 caaattttga tcttgtgaag atactcgaat atagccaatt tttcccataa aaaactcctt
     2521 tttaaaagtg atataattta catgtaaaat ttacttgtag ttttactgct atcatagcat
     2581 ttttaaaaat aaattttatg tagacttaaa atttacttca tggagggttt aaatggatga
     2641 aatcaaaagt aacaaaaaag catttggcat ttatttagga gaacctagta cagaacaatt
     2701 aaaccaattt t
//
LOCUS       DANKKG010000209         2700 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000209 DANKKG010000000
VERSION     DANKKG010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.211"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(<1..1013)
                     /locus_tag="PW004_002723"
     CDS             complement(<1..1013)
                     /locus_tag="PW004_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016633586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2727646.1"
                     /translation="MAGTIKEIAEKAGVSRGTVDRALNDRGRINPEVAERIRAIAKEM
                     NYVPKKKKVIKETTLKLGIVTQLAKSSFMIPIRSGLESIIGDLKKRNIDCFLEEVDGV
                     DEAAQLQALDRLVELGVKGIAIMPVDSDGIREKINQLTDQGILIITFNSDILGTNRQC
                     FVGMDNFKSGKTAAGLLGMLTKGKGKVLAITGYFGNSVNSLRVAGFVEELKESFPELE
                     LTGVQSSFDSSAEVEKILSNTLENYSNLDGVVVFSGGQAGIARALEKIDESKRPFVII
                     YDLTKNNIEMLKNDQVDFLIDQDGFVQGYRSLLMLANQLQNNKTVTKDALFTDIIIKT
                     KYN"
     gene            complement(1115..1312)
                     /locus_tag="PW004_002724"
     CDS             complement(1115..1312)
                     /locus_tag="PW004_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727647.1"
                     /translation="MKFICLSCGEKESIPMDVVKFLDDADIANDPNNPPQFTCEKCGK
                     EMYPEYYRNALGIEFKISDVQ"
     gene            complement(1284..2603)
                     /locus_tag="PW004_002725"
     CDS             complement(1284..2603)
                     /locus_tag="PW004_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016372324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HDL2727648.1"
                     /translation="MKANILEAIFFDEHHNWDNFVEKYGRKIRLVVKREVNKFRYCGD
                     IQKGYRLFVCEGCHQTKFVPLKCRGKFCPTCATGESQRWSAVVANDMFKVTHRHVIFT
                     IDEGLREIFMMENYRDSLLKGLMDEAAKIILAYFEKRKVKAGIVATIHTFGSKLEFNP
                     HVHMVVTMGGLTRDGSWEEYDYLPFSMLRKYWKNAVLKLIRRTLGEWDKQRVQSRLQA
                     AYKNNGEGFYVNAPKRSRTNLKSLLQYISRYMKRGPIALSRILMYDGDTVMFNYMDKR
                     TNTKETMTMSIDEFIAALIRHIQNKNFKTIRRYGIYSRRIKTLMKKVMKEYQKRVKKL
                     FINLNEALRPKSWEERITEEFGENPLECSCCGEYYEFVGMSVRKNGRLVIKYAKDNHA
                     KRFMREENRKIDQKEFRQQYEEEKEKTFKAIRWDWERQREIYMSKLW"
BASE COUNT          704 a          645 c          413 g          938 t
ORIGIN      
        1 atattgtatt ttgtcttaat aattatatca gtaaatagcg catcttttgt aaccgtttta
       61 ttattttgca gctgattcgc taacatcagt aatgaacgat agccttgaac aaatccatct
      121 tgatcgatta gaaaatcaac ctgatcgttt ttcagcatct cgatattgtt cttagtcaaa
      181 tcataaatga ttacaaaggg ccgcttgctt tcatcgattt tttccaacgc tcgggcaatt
      241 cccgcttgtc cgccagaaaa gacgactacg ccatctaagt ttgaataatt ctccaaggtg
      301 ttgcttaaaa tcttttcaac ctcagcactc gaatcaaagc tgctctgaac acccgtcagc
      361 tccagttcag gaaacgactc tttcaattct tcaacaaagc ctgccacccg taagctgttg
      421 acgctatttc caaagtagcc ggtaatcgct agaaccttgc cctttccttt tgtcagcata
      481 cctaacaatc ccgcagccgt ttttccgctt ttgaagttgt ccatcccaac aaaacactgt
      541 cgattcgtac caagaatatc cgaattaaag gtaatgatga ggatgccttg atcggtcaat
      601 tgattaattt tttctctgat accatcacta tctactggca tgatcgcgat cccttttact
      661 ccaagctcca ccaatcgatc caacgcctgc agctgggccg cttcatcgac cccgtctacc
      721 tcttctaaaa aacaatcaat gtttcgcttt tttaaatccc cgataataga ttccagacct
      781 gatcggatcg gaatcataaa agaagatttc gccaattgag tgacaatccc cagcttgagc
      841 gttgtctctt ttatgacctt ctttttcttt ggaacatagt tcatttcctt ggcgattgct
      901 ctgattcttt ctgcaacctc aggattgatc cttcctcgat cgttcaatgc ccgatccact
      961 gtcccccttg acactcctgc tttctcagct atttctttaa ttgtacccgc catcttgcct
     1021 ctcctttttt cctattgtgt ttattttatc acaaaactta agaaaaccac cggagaatga
     1081 attctccggt ggactttttg tatgattaac cttcttattg aacatcactg atcttaaact
     1141 ctattccaag ggcgtttcta taatattctg gatacatctc cttcccacat ttttcacagg
     1201 taaattgggg aggattgttc ggatcatttg caatatctgc atcatctaaa aattttacta
     1261 catccattgg gattgattct ttttcaccac aacttagaca tataaatttc acgttggcgt
     1321 tcccaatccc agcgaattgc tttaaaagtt ttttcttttt cttcttcata ctgttgcctg
     1381 aattcttttt ggtcaatttt tcgattctcc tctcgcataa accgctttgc atggttatct
     1441 ttcgcatatt taataactaa ccgtccattt ttgcgcacac tcatcccaac gaattcatag
     1501 tactcaccgc aacaagaaca ttctaaggga ttttcaccaa attcttcggt tattctttct
     1561 tcccaggatt ttggtcttaa ggcctcattc agattgataa acagcttttt tactcgtttc
     1621 tgatactcct tcatgacctt tttcattaat gttttgattc tacgactata gattccatag
     1681 cgacgaatcg ttttgaaatt cttattttga atatggcgaa tcagtgcagc tatgaattcg
     1741 tctatactca tggtcatcgt ctctttagta ttcgttcgtt tgtccatgta gttgaacatg
     1801 acagtgtcgc catcatacat taagattcgg cttaatgcga tcgggccacg cttcatataa
     1861 cgactgatgt attggagtag tgactttaga tttgtcctgc tccttttagg agcatttacg
     1921 taaaaccctt ctccattgtt cttgtacgca gcttgtaatc ttgattgaac tctttgcttg
     1981 tcccactcac ctaacgttcg tctaattagt ttcaatacag catttttcca gtattttctt
     2041 aacatagaaa aaggtaaata atcgtattct tcccaacttc catctcttgt taagccaccc
     2101 atagtaacaa ccatatgaac atgcggatta aattctagtt ttgatccaaa ggtgtggatt
     2161 gtagctacta tacctgcctt cacctttctt ttctcaaaat aggcaagaat aatttttgcg
     2221 gcttcatcca ttagtccttt cagtaaggaa tctcgatagt tttccatcat aaagatttcc
     2281 cgtaacccct catcgatcgt aaagattacg tgccgatgtg tcactttaaa catatcattc
     2341 gccacaaccg ctgaccacct ttggctttca cctgttgcac acgttggaca gaactttcca
     2401 cgacatttca agggaacaaa ctttgtttga tgacatccct cgcagacaaa cagtcggtat
     2461 cctttttgaa tatctccaca atatctaaac ttgtttactt cccgtttgac gactagccga
     2521 atctttcgcc cgtatttctc aacaaagtta tcccaattat ggtgttcgtc aaaaaagatt
     2581 gcttcgagaa tattcgcttt catagtaata ttttacttgt tcaaaaacaa aatgaaaagg
     2641 gctaaaagac tggtagtgca gcctttttag cccttaactt tttatacttt ctttactgtc
//
LOCUS       DANKKG010000210         2692 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000210 DANKKG010000000
VERSION     DANKKG010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.212"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..1568
                     /locus_tag="PW004_002726"
     CDS             <1..1568
                     /locus_tag="PW004_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HDL2727649.1"
                     /translation="SLRENGQKNPVWIHFGGGNLYRGFHAEIAQRLADLGELSTGVIV
                     CETFDEEIIERAYQDFDDLILEVVMPSSGEFVKRILAATADSLYCHPDCSEHFDKMIR
                     HFENPSLQLVTFAITEKGYRLKDTNNKYIGPIAVDIKNGPKKPSHTMSILAALLYKRF
                     LSGAWPIALVSTDNFSQNGSQLRTSLLTIAKEWQKEGYVQNTFIQYLSNEEIVAFPWT
                     MIDRITPNPSLSVSKMLAKEGFSNMSIFQTAKGNNIAPFVNTEDIHYLIMEDHFPNGR
                     PKFEKAGVFLTDRATVDKADTMKVTTCLNPLHTSLAIMGCLLGYRSIAEEMKDPDLSL
                     LVSEIGYNEGLPVVESPGIIDPKIFLEEVITVRFSNPNIPDMPQRIATDTSQKVAIRY
                     GETIKKYMEIEVKDPQTLVFIPLTLAAWLRYLIGIDDQGKPFIPSPDPLLTDFQHKLS
                     DIQLGTQDTSHIHEKMAPILSNKEIFGVDLYQAELGRKIEEMFIKMVAGPNAVRKTLQ
                     DYLQEHGGNLSWK"
     gene            1559..2641
                     /gene="uxuA"
                     /locus_tag="PW004_002727"
     CDS             1559..2641
                     /gene="uxuA"
                     /locus_tag="PW004_002727"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDL2727650.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
BASE COUNT          889 a          473 c          567 g          763 t
ORIGIN      
        1 catcattaag agaaaatgga caaaaaaatc ctgtctggat ccatttcggt ggcggaaatc
       61 tttatcgagg atttcatgca gaaatcgctc aacgtttagc ggatctaggc gaattgtcca
      121 ctggtgtcat tgtttgtgaa acattcgatg aagaaataat tgaaagggct taccaagatt
      181 ttgatgattt gattcttgaa gtggtcatgc caagcagtgg agagttcgtc aaacgtatat
      241 tagctgcgac agctgacagt ctgtattgtc atccagattg ttctgaacac tttgataaga
      301 tgattcgaca ctttgaaaat cctagtttac aattagttac ttttgctatt actgaaaaag
      361 gttatcgatt gaaagataca aataataaat atataggacc gattgcagtc gatataaaaa
      421 atggtccaaa gaagccatca catacaatga gtattcttgc tgcgctactt tataaacgtt
      481 ttttatctgg agcttggccg attgcacttg tctctacaga taatttttca caaaacggta
      541 gtcaattacg tacttctttg ctaacgatcg caaaagaatg gcaaaaggaa ggttatgtac
      601 aaaatacatt tatccaatac ttatcgaatg aagaaattgt tgcgtttcca tggacgatga
      661 ttgatcgtat caccccgaat ccttccttgt cagttagcaa aatgttggca aaagagggct
      721 tttcaaatat gtcgattttc caaaccgcaa aaggaaataa tattgctcca tttgtcaata
      781 cagaggacat ccattattta atcatggaag atcattttcc caatggacgt ccgaaattcg
      841 aaaaagctgg agtattcttg actgatcgtg caacggtaga taaagcagat acgatgaagg
      901 tgacgacttg ccttaatcct ttgcatacgt ctttagctat tatgggttgt ttactggggt
      961 ataggagcat agctgaggag atgaaagatc cagatttatc gctgctagtt tctgaaatcg
     1021 ggtacaatga aggcctccca gttgtggagt ctccaggtat cattgatcca aagatctttc
     1081 tggaagaagt aattactgtg cgcttttcta atccaaatat tcctgatatg cctcagcgaa
     1141 tcgccacgga tacctctcaa aaggtggcga ttcgttatgg tgagacgatt aaaaaatata
     1201 tggaaataga agttaaagac ccgcagacac ttgtattcat accattgact ctcgctgctt
     1261 ggcttcgata ccttattggc attgatgatc aaggaaaacc attcatcccg agtccagacc
     1321 cactattgac agactttcag cataagcttt ccgatattca actgggtacg caagatacat
     1381 cacatattca tgagaaaatg gctcctattt tgtcaaataa agaaattttt ggtgtagatc
     1441 tttaccaagc agagcttggc agaaaaatag aagaaatgtt catcaagatg gtagcaggac
     1501 cgaatgccgt acgaaaaaca ttacaggatt acttacaaga acatggagga aatttatcat
     1561 ggaaatgact tttagatggt acggatcaca ggaagaaaag attaccttag atgaaatcag
     1621 acagattcct gcggtcaaag ggattgtagg cacattgatg gatataccag taggagaggt
     1681 atggcctagg gagaggatca aagaactaaa agaagaaatt gagtctcgtg ggttaacact
     1741 aaaagtaatt gaaagcgtca atgtgcatga tgccattaaa attggattac cttcccgtga
     1801 gcagtatatc gaaaactata agcagacaat ccgaaacttg gcagaatatg gaatagaagt
     1861 catctgctat aattttatgc ctgtatttga ctgggtgaaa tctgaccttg actaccgctt
     1921 agaagacggc tcatccacgt tagcttttat atctgatgat attccagatg atccaagaga
     1981 aatcgtcgcg cgcatcgaac attccagcaa tgaatttgaa ttgccgggat gggaacctga
     2041 gcgcttagct catatcaaat ccttgcttga agcctatgca tcagtagacg aagaaaaact
     2101 tcgagaaaat tttgcctatt ttctccaatc aatcatccca acatgtgaag aggtaggggt
     2161 caaaatggct gtacatccag atgatccgcc atactcgatt tttggattac ctcgaatcgt
     2221 aaagaatcga gaagatttag attggttgtg ccgcatagta gacagccctt ataacggaat
     2281 cactctttgt acagggagta ttgcagaaga tccagaaaat gatgtatatg ggattttagc
     2341 agaattttgt caaagagatc gaatcccatt tgcacatgtt cgaaatatta aatttttagc
     2401 taatcgagat ttctatgagg caccgcataa atccgaatac ggttcattag acatgtaccg
     2461 catactaaaa acaatgtatg acaacggatt taacggatac atccgtcctg atcatggaag
     2521 aatgatctgg aatgagacag gaagaccagg atacggctta tatgatcgag cattaggtgc
     2581 tgtttatcta aatggattat gggaagcaat cgaaaaagga aacaaaagta aagataatta
     2641 gtgaaaaaaa ctgtaatttt attatgtaaa taagaaggat gttcaaattc tt
//
LOCUS       DANKKG010000211         2673 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000211 DANKKG010000000
VERSION     DANKKG010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.213"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            16..462
                     /locus_tag="PW004_002728"
     CDS             16..462
                     /locus_tag="PW004_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDL2727651.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            735..2087
                     /locus_tag="PW004_002729"
     CDS             735..2087
                     /locus_tag="PW004_002729"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HDL2727652.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            2258..2545
                     /locus_tag="PW004_002730"
     CDS             2258..2545
                     /locus_tag="PW004_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727653.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT          935 a          445 c          534 g          759 t
ORIGIN      
        1 acgtggggtg tttacatgga aaataagtta gtcgaagagg ttatcgaaaa gttaaaaaaa
       61 gagaacattc gaatcacacc tcaacggtac gcggttttag aatatttgat tgaacatcat
      121 tcccatccaa cggctgatga gatttataaa gcacttgaac atcggtttcc aaatatgagt
      181 gttgcgactg tttacaataa ccttcgctta tttacttcga ttggatttgt tcaagaaatg
      241 aagtacggcg atgcttctag ccgatttgat ttcagttcaa agcgtcatta tcatgtaatt
      301 tgtgaaaact gcggtaagat tgttgatttt cattacccag ggcttgaaga tatagaaatg
      361 gctgcaagca gactgactga ttttgagatt aatgaacatc ggttggaatt gtatggactc
      421 tgtccggatt gtaaagagtc agcaaaaaag gcgcaaatgt aaaaacagtt tctacaagtg
      481 ttatacctaa aaaaagctag ctgtgtaaaa aaaacgctag cttttttatt tttttgtctt
      541 ttattcacaa tcttagatat aaaagtgaat attattaaaa taagaatctt gtagatattg
      601 ggttcttatg cttgaaaagg aatgaaacta caaacaaacc tttgtgaatt cacaagcatt
      661 aaactgttgt atccgtttta tttctgttat agtagttttg tcgaaggaca aaatgttaat
      721 cggagggacc ataaatgaaa gtagtagtaa ttggctgtac tcatgcaggt actgcagctg
      781 taaaaagtat tttaaaaaat catccagaag cagaagtgac agtatatgaa agaaatgaca
      841 atatttcttt cttatcttgc ggtatcgcac tttatgtcgg cggagtagta aaggacccag
      901 caggcttgtt ctactcaaat cctgaagaat tatcttcatt aggagcaaca gttaaaatgg
      961 aacacgatgt aacagacgtc gatacagaaa ataaaacagt aacggcaaaa gatctgaaaa
     1021 caggagaaac agaaacggtt tcttatgata aattagtgat gacaactggt tcatggccta
     1081 ttatcccacc gattagtgga atcgatgcga aaaatgtact cctatgcaaa aactacaatc
     1141 aagcaaatga aattattgca caagcgaaag aagcaaaacg tgtagtaatt gttggtggtg
     1201 gatatatcgg tattgaatta gtagaagctt ttgtcgaatc tggaaaacaa gtgacattga
     1261 ttgatggatt agatcgcatc ttgaataaat atctagataa accattcaca gatatcttag
     1321 aaaaagaatt gacagaccgc ggtgttactc ttgcgctagg cgaaaatgtt actgaattta
     1381 tcacagatga agaaggaaaa gtgaaacaag tagctacacc aacagataca tttgatacag
     1441 atatggtcat tttatgtgtg ggcttccgtc caaatacaaa actgatcgaa ggaaaagtag
     1501 atacactgcc aaacggggca atcaaagtga atgaatacat gcagacaagt aatccagaca
     1561 tttttgcagc tggagactct gcagtagtca actacaatcc aagtggcacg caaaactata
     1621 ttcctttggc aacaaatgcc gttcgtcaag gtttgcttgt gggaaataac ttgacagatc
     1681 ataaaatggc gtatcgtggt actcaaggaa cttcaggact ttacctattt ggctggacga
     1741 tcggttcgac aggtgtgacc aaagaaagcg caactttgaa cgatctagaa gtccaagcga
     1801 ctgtatttga agataattat cgtccagagt tcatgccaac tactgaaaaa gtaatgatgg
     1861 agctagtcta tgaaaaagga acgaatcgta tcgtaggtgc tcagttcatg tccaaatacg
     1921 acattacaca atcagcaaat accatgtctt tagctgttca aaataaaatg actgtagaag
     1981 atctcgcact ttcagatttc ttcttccaac cacattttga ccgcccatgg aattacttga
     2041 acctacttgc acaagcagcg ctaggcgaaa tcgaaactgc acaataaaga aaaagggttg
     2101 tgacacaatt ttgtcacgac cctctgtctt tttttgtgaa aagtacaatt agttgtcttt
     2161 cttcgaattt tttgtcacaa taagcagcaa agataaagct gttacataaa agaacaagca
     2221 gctttatttg attttgaaaa gaaaggtgag aaagaagatg aataaagaat attttgtcca
     2281 acaagatgca tttttagaag attataaaag tgcttcgaag aaattaacga tcaatattga
     2341 tgagttggca gatcaagtac tctctcagtt gaatgaaacg caagaggaat attacacgat
     2401 gccagcaaat gacgccaaag ataatgtttc tcatcgtttt cctttcagta gacgcatcta
     2461 ctcagatgaa aatgacggca cattgaatac agagtacaca tatgaaggtc agccatatgt
     2521 gctagaaact gatgatgatc tttaaagaca gtttttgaaa aggccgctca tccttaagtg
     2581 atgacaaaaa atcatcacaa ttaccgatga ctgtctcttt actatctttt aatcagaaaa
     2641 aggggttatg acaagctttg tcaacccctt ttt
//
LOCUS       DANKKG010000212         2650 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000212 DANKKG010000000
VERSION     DANKKG010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.214"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(<1..962)
                     /locus_tag="PW004_002731"
     CDS             complement(<1..962)
                     /locus_tag="PW004_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2727654.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHT"
     gene            complement(1261..1896)
                     /locus_tag="PW004_002732"
     CDS             complement(1261..1896)
                     /locus_tag="PW004_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727655.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(2009..2644)
                     /locus_tag="PW004_002733"
     CDS             complement(2009..2644)
                     /locus_tag="PW004_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HDL2727656.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
BASE COUNT          761 a          444 c          399 g         1046 t
ORIGIN      
        1 gtatgcctta gtccatgcaa agtaaattgc ttttcaatcc ctaattcttt taattttttg
       61 cgcaaatatt tgctgacggc gtttgatgaa actaatccat tttttaagtt gaaaaaaaca
      121 aaattatgag ggtttttgat ttcaaaattc tcgaataatt ctttttgatt gattttaaac
      181 ttttttaaga gatcaatcgt atgtttatca atggaaattt tcctatttga agtttcgttt
      241 tttgtttttc cccattcatt taatttataa ttccatgttt tattaatagt gatgatttga
      301 tcttcgaaat cgatatcttc ccatgttaat cctagaagtt cagcaaatcg cattccagtg
      361 gcaccagcaa ctaacacgag cataggagaa gagtactgcg cagaaaggtt ttcttctacg
      421 agtttcatta aagatttaaa ttgatcataa tctaaatatt tatcttcttt tggcttcaat
      481 gaatcttttc ctttgattac tgcttttcta gttggatcaa acggaattaa tccttcttct
      541 acggcatctt tcaacgacgc tctaatatgg ttattaaact taataacggt agattttaca
      601 tggtctttag catacttatt taaaaagcgt tgatatccca ctctatctaa atcagaaatc
      661 aataccgctg gcatatattt ctttatgttc attaacgtat cttcgtattt tctataagtg
      721 ataggagata ctgttccttc tttataaagt tgcatccaat cctcaaagta atctgataat
      781 aatagatttt tgcgatcccc tttaaggcct ttgttcaagt tatattctaa ttcgttggcg
      841 gcatcttttg cttctccttt tgtcttaaat cctgattttc ttatcttcgt gtattttccg
      901 tcatcttttt tgtatgaaat ttcgtattgc caactatttc cacgtttgac taatcttgcc
      961 ataattgaat cagctctctt tctctgatac aataggaact ataaagaagc ccattgtttg
     1021 ggttttatta ttttttgcac atcctttttg cttgccggcg caaggatgtg ctatttgtcg
     1081 ttttaatggt ataattggat gtgtattaga tcatccaatc agaggtgttt attatgatta
     1141 aagtgatttt taaaagaata actgaacttc cagagtttag attatttgtt gattattggt
     1201 atgtctggtt ttttattgtt gcattgctga taggtctaga tgtcatatta gaaagaaaac
     1261 ttaagtaaga gttgagttaa taaattgaat tcctaaatga gatattttgt agcccatatt
     1321 ttgtttgccg acatcaccta atactatgga gctaatcgtt ttttctaaca gaccaaaatc
     1381 agataatcta tttaagctac catctattag ctcttgtgaa tagccagaca tgctgatatt
     1441 tccagtgaaa ttttcgtttc cacgactcat taagcatgct aacaattgaa tctcaatcga
     1501 tgttaattga ttcagtacaa gaagataata ctcctcgtta tcccatgaag cgttgttaat
     1561 tcgaagtaaa caattcttat aaagattttt atacatatct attttatttt gtgcctttga
     1621 tttttctacc tcgtcattta tcccttcgaa tattccaatg agttggtcct tgttctctaa
     1681 gttgaaaaca tcttctggaa tttgatcgcg aatgttttct aatgattcgt ttaaattttt
     1741 gtagaatttc tcgatgcgtt tgaaacgctt ctcgttttta gacccaaaat aaagtgactg
     1801 cacaacgcca ccaatataag gaattgctcc aataccgcct tcaactatta attcaagata
     1861 ttcttttttg gttagttttt cattgctatt atccatagtt acctcctatt ttgtcatttc
     1921 ctttacaagc attgtgactc ctgatggcac tcttttgtat aggttttatt attgtgcaca
     1981 atctaactct tagtggggcg tggttcattt attttttact tactttacta ttaatcatat
     2041 cccattgagc ataggatttt tctatgttcg atcctcttga actaatttct cctgtaaaag
     2101 ttactttatc gccctttgta aaggtatcag cattgtttaa gtccttcgca aaaattacat
     2161 atgaaacttt aggtgttgca gaaatatcat tccaactttt atcatcgtac tctttgtctg
     2221 ctataatggc tattctagta gtcattgatt cgataacagt tccttcaaaa gtatatgttt
     2281 taccactaat atatttagta aaagctgtat tttgctcagt agctggagta atagaataat
     2341 aactatctaa gaaagcaccc cagtcatgat tatttgtctc taaaaattct tcaaattgag
     2401 ttaggctttt attgctgctg ttagatgtag tagtttgagt atcagatgat gagctttcct
     2461 gtttgttatt gtcatttgcc tcgtttgatg aggaaagcga gaaacctacg gtagtaaaaa
     2521 taatcccgat aataaggaga atcataggtg ttttcttatt tgtttttttg ataaatctta
     2581 caattaacat gattaaacct ataataaata aaacaatacc agtaaaacca aatataccgt
     2641 ccattaatag
//
LOCUS       DANKKG010000213         2646 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000213 DANKKG010000000
VERSION     DANKKG010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.215"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..176
                     /locus_tag="PW004_002734"
     CDS             <1..176
                     /locus_tag="PW004_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2727657.1"
                     /translation="QELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKAND
                     LLFCYLLLLHEHN"
     gene            complement(257..592)
                     /locus_tag="PW004_002735"
     CDS             complement(257..592)
                     /locus_tag="PW004_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727658.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(659..2029)
                     /locus_tag="PW004_002736"
     CDS             complement(659..2029)
                     /locus_tag="PW004_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HDL2727659.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(2107..2643)
                     /locus_tag="PW004_002737"
     CDS             complement(2107..2643)
                     /locus_tag="PW004_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2727660.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
BASE COUNT          744 a          570 c          416 g          916 t
ORIGIN      
        1 ttcaagaatt atggaatgaa ttaggcaatg tcagctctgc tgcaaaaaca ctgtttcttc
       61 atcgtaatac tctaaaatat cgaatcgaaa aatttcaaga acagtctggt ttcaatctaa
      121 aaaaagccaa cgatttactg ttttgctatc ttttattgct tcatgaacat aattaaagat
      181 agattaaagg ggttgtgaca ataattttgt cacaacccct tatttcgaat gaatagtgtt
      241 caaaagctag tttctcttac atcatttttt caacaaaatg cattagccgg tcttttacat
      301 cttcggttga atctttgatt gaatcaccat aaacagagat tttctttttt cctgatttga
      361 tttttgctaa cattcccatc aaagaatcca agtcactatc tgaaagacca tctacgattt
      421 ctagtaattt ttcatttcca tcaaatttgt catagacgaa tttttttact ttcgtccgag
      481 tagtcatatg atggatcttt tccattactt ttcccgatac aacgactgct gtcgctacac
      541 tggcagcggc tgctacgctt aatccgattg ttacttttgt tgatgttttc ataagtatca
      601 gctccttttt atctatctcc atcatagcat agacaaaaaa ataaacgaac taatccactc
      661 agtgaaatct caatccttta gggaaaaagt tctttactgt tccttttact tgtttcccaa
      721 atccgacagg tatcttatct aatactagca aaacccatcc ttgattccca tcacgttgaa
      781 atgtttctcc tgctgtgtaa gattgccatt ccttttgagt gatcggcaag caaggtatat
      841 tctctatctt ttttgtagca agcgctaatg catagctagg ttcaaatcga ttctttttga
      901 aatctcctaa atgcaaacct gtacgtacga cttttaaacc atcgagagaa ggggctaact
      961 caggaacttc ccaaagatgg tcgttgaaca ctaaaagtct tccagtagct tcataatgaa
     1021 aatcatttga aaactccgtc cagagttttt cctgctcttt tgtcatttgc actttacttt
     1081 tttttcgtgt cttcttttct ttatgcatct gattttgtcc atgaaaagtc agctttgcaa
     1141 caaaatgtcc ttcaccctga tctttatgag gccatatacg tattgttttt tccaaaccag
     1201 ctacgcttcc ccattcagaa cgtcctgaac taacggattg agtcaaagga atctcctcaa
     1261 tcgttactgg ataattttct acaagccagc taatgatctc ttcattttct tctggtgcaa
     1321 acgtacaagt cgaataaatc agttgtcctt tattttttag catttttatt gctgaagaca
     1381 aaatctcttg ctgacgtttt tgacagtata gcggagattc ttctgtccac tcttttattg
     1441 catttggatc ttttcggaac attccttctc ctgaacaagg agcatcaacc acgatccgat
     1501 caaaaaagcc cgaaaaatga gggacaagtt ctgcgggtgc atgatttgtc actattgcgt
     1561 tcgaaactcc ccatcgttct atattttctg ataaaatctt cgcacgtttc ggaaagatct
     1621 cattcgtcac taataaccct ttccccttca tttgagcagc taattgggtt gatttaccac
     1681 cgggagccgc acacaaatcc aaaacttttt caccgggttt tgctgcagca gctgttccaa
     1741 cgatcattgc acttggttct tggctatatt catacccagc ttgatggagg aaagactttc
     1801 cattgacagt gccaagaaat ccctcattgc tgtagggagc gggttgtaat tcttcgctat
     1861 ggtaagtttg gaccatgtcc aatcctgccg gttttagtgg attccaccga aatccttttt
     1921 tcacgcttcc ttgttcaagc gcagaaaaaa aatcggatgc ttcttcgcct aaaagaagac
     1981 gatatttttt gataaattgc tgtggtaacg ttgtcgcttc ctccttcatt ccaatgtttc
     2041 gttagacatt ttacaatctt ttttacgaaa aataaaggtg gctgatctac tttttcatgt
     2101 tgctgcctaa ggtgactgtt cctttaagat attttccaag tattcatccg tcacctcgtt
     2161 tttatcccaa acagcgttca tcatttcaag aaaatcttga gaagaatcat ctgagcgcaa
     2221 acttcccaat gcattaagcc ataccaattc aagcgacttt tcttctttga tcttcgtgtg
     2281 acctttgaca tatgagaaaa atggccgctt aaatagcgaa tccatgtcta aatcagattt
     2341 tgcaatgatt tcacgaatac tttcatggat agaaaaaaaa tctttcaata agtagacaat
     2401 gcttaaaaca tcatcggaac tgcattcagc taagtagttt tcaaaataaa cagtgatttc
     2461 ttctaatatc ttctctttta acccgctgat tgatttgaaa tgtgtataaa tcgggtatgt
     2521 agaacaaccg cagaactctg ctaactctct tgcagtaatt gacttcattc catattcttc
     2581 cacgtatacc atacttttga acagcaaata ttctttacta taatttgtta ctctccccat
     2641 cattct
//
LOCUS       DANKKG010000214         2645 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000214 DANKKG010000000
VERSION     DANKKG010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.216"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(61..399)
                     /locus_tag="PW004_002738"
     CDS             complement(61..399)
                     /locus_tag="PW004_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HDL2727661.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            complement(415..618)
                     /locus_tag="PW004_002739"
     CDS             complement(415..618)
                     /locus_tag="PW004_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2727662.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(633..1409)
                     /locus_tag="PW004_002740"
     CDS             complement(633..1409)
                     /locus_tag="PW004_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HDL2727663.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            1708..2028
                     /locus_tag="PW004_002741"
     CDS             1708..2028
                     /locus_tag="PW004_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2727664.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            2046..2477
                     /locus_tag="PW004_002742"
     CDS             2046..2477
                     /locus_tag="PW004_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HDL2727665.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKISILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
BASE COUNT          819 a          452 c          415 g          959 t
ORIGIN      
        1 ctgcttcccc aaagtcctaa agcgtagatg attagcggtg cgctgaacac tagcgctcta
       61 ttaaattttc ccatccgctt gcctccttaa gatttccgaa aaatttgttt ctaaaaattc
      121 aagtgtttta cttcttaaaa atagatatgt gtctcttcct tcaactggat aatagacaaa
      181 tccatttttg tttttctcga tatcgataat atttctatat cttgggttct ttaaaactct
      241 agaagtaaac caatcatatt ttctgttaat ccgttctagc acttctggca acgtcatcca
      301 tctaccagta tcatcagctt tttttaactc ctcataatcc acttgggaga taattacata
      361 gccttctgga attgggattt ttgcttctag atattgcatt agctgttcct ccctttacaa
      421 ttcgtacata gtgataatcg aatctataat tctgtttgct tctgcagaag tctttttacc
      481 gtttaaaatt aaagataagt agcttttgct aattccaaat ctttcagcaa gcatggtgta
      541 agttaagaac tttgaacttt cgacatattc tttgattttt tctctatctc gttgagtgat
      601 ttctgcaatg tcagtcatac taaaactcct ttctaaccaa tttcctctaa atccatttga
      661 gggtaatatc cttctttttt tagtaattcg tagataaata gacgcccttt ctgtgtccat
      721 ttggtattca ttacaatttt agtgccacca tcggctttcg ggatctcagt tgtatgagat
      781 tttgtgtatc cttggttcat gtgttttttg cacaataacc attggttacc gacttttttc
      841 tgaataccta gtttatgaag taatttattc atctgttgtg gagacatccc ataatctgct
      901 gcaatctgac taattgttac tgaatctgta gaagataata tgctatctaa ataggagatt
      961 ttaggttcgt actctgcaat cttttgttct gcgattaatc ttccagttct ttcttctttt
     1021 agttgagttg ctaattgaat gattgtatct ggattaagca aagcttcttc tactttttct
     1081 ggagttagat aacctccatg ttttctaatt gctggcaaca cttcacttgt tacccaacgt
     1141 ttaaattttt tggcagaggg aagttttgat tttaagatta aactgtacaa accagattcg
     1201 ttgatgattg tcatctctct tgattgacct gaggtcgtga ttcgcgaccc catcttatct
     1261 tccaaatcta catgccgcga caaagcatct ttagtgtttg aataacccaa aacactcgca
     1321 acgtctttgc ctacaaaata tggttcatca tttactaaaa ttgtccgaac ttcgttttgt
     1381 tcgaaattaa aaatttgcgg tgtgttcata tttctcattc ctttctttta tattcgtaaa
     1441 caaatttaac aacttttttc taaattcaat tgacagtatc tgagtaatac tctatactaa
     1501 gagcataatt aaataagaca taaaaacatt gattttaaag ctttcttggc ggtctgcatt
     1561 tacatatcaa tagtgttttt tgttgtcttt ttagttgtta aatctgttta caagatatag
     1621 tatagctcat tactcagaat tagtcaaatt atttctgcgt atttttctaa actttttttg
     1681 taaacattta gaaaggttgt tttatcgatg aatacctatg aaataataaa agacttggcg
     1741 aagaggaaaa agatatctat acggcagtta gaaattaatt ttggatattc taacggttat
     1801 ctagcaaagt ggaaaaataa tactcctaat tcagatgaat taagtcggat tgctgactat
     1861 ttcgatgtct ctgtagacta ccttctaggt agagaagaaa gagaaacccc taaacatgtg
     1921 gatttatcag aagacgatac tgttttttct tttgatggaa aagaaatatc taaggagaca
     1981 atgcgtaaag cgattgcaat tgctaaagct ttagaggaaa atgaatagtt ggagtgatgg
     2041 tttgtatgta tttgaaattg aaagaaatgt tgagcgaata caatttgaaa gtagtttata
     2101 tggagatgaa agagcctggt ttctactatc caaaacccag aatcattttt ttgaatgaaa
     2161 atctatacgg tgaaactgca gaagcctttc atttatctca tgaacttgca catttcagcg
     2221 cctctcattt tgaattttca gttttgtacg atacttctac aacttttcac tcaaaatttg
     2281 aaacagaagc agataaaata tctattctaa ttttattaaa tatctatatt gagaacgaac
     2341 tgactgatga atctcagttt aatctcgaaa aatttatgga gtattattct attcagaata
     2401 agctcagata cacttgttat gctgtctgcc aatgctattt taagaaaaaa tattcttacg
     2461 caaggcaata tgtatgaata catcaagaat tattaaaaaa accgatttca gcaaaacatt
     2521 aaaaaagccc gtgctgcaac acgggctctt tcctcatata tgagcttcta caaaaaaaat
     2581 catatcacag aaatgaggaa gaaaaaatgg aaaaagagat tttaataaaa tctccaggaa
     2641 aaacc
//
LOCUS       DANKKG010000215         2641 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000215 DANKKG010000000
VERSION     DANKKG010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.217"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..357
                     /locus_tag="PW004_002743"
     CDS             <1..357
                     /locus_tag="PW004_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727666.1"
                     /translation="IATHAEVKNYSEAVRFLCMNYDSEVEKKVNSNQAKLNAMGKEIS
                     ILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVEGKIKANQTKKYSPKIKKTISES
                     VTTKSEVFDPTRFLKR"
     gene            377..1966
                     /locus_tag="PW004_002744"
     CDS             377..1966
                     /locus_tag="PW004_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727667.1"
                     /translation="MSKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEKERRTPEE
                     EQELRLIKNALEDFYIPEGETFSESKTGKEFSETSNEAQSLMKHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKVLTHSEQKEYQRLNQAIKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQTFQRGQQKGSIIYQDVVSHDHSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKKNTRKPYTEIENGVSYIVPKG
                     KRRQATLDEMKTNYVHELERFSYEKNRMGMEKRNLLTRKSDLRNTLTKVVKEPVRYDR
                     HAMSLLTEVYKNLPKKRSEWHYGDENRTKLSPYTREKLDELTQYVLTKEPGYQEYIQI
                     SKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKKQDSQIKPLIERMDQ
                     MQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEEK
                     YHASKAYEEQQRRIQMEQERQ"
     gene            1978..2313
                     /locus_tag="PW004_002745"
     CDS             1978..2313
                     /locus_tag="PW004_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727668.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLINEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
     gene            2454..>2641
                     /locus_tag="PW004_002746"
     CDS             2454..>2641
                     /locus_tag="PW004_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727669.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQSGDI
                     LTLNLTTKTFVDAAEDYP"
BASE COUNT         1047 a          404 c          540 g          650 t
ORIGIN      
        1 attgcaactc atgcagaggt taaaaactat agtgaagccg ttcggtttct atgtatgaat
       61 tatgattcag aagtggagaa aaaagtaaat tccaatcaag caaagttgaa tgcaatggga
      121 aaagaaattt ctattttaac cgaaatggtt tcagagtttg cggatctcca tttgaaagat
      181 acagggattt taattggtac aaattcaact gtttaccaag aagcaaaaga gcgtgtggaa
      241 ggcaagataa aagcaaatca aacgaagaaa tattctccta agataaagaa aacaataagc
      301 gaatcagtga ctacaaaaag tgaagtattt gatccaacaa ggttcttaaa acgataaaaa
      361 ttagaaggag aatgtgatga gtaaatcacc tgcagtcatt ctcacttgtc aatttacctt
      421 gcccaataaa aaatcttttt caacatacat tgattatatg acaagagaaa aagcgctgga
      481 agaaaaggaa cgacgtacgc ccgaggaaga acaggaactg cgtctaatca agaatgcttt
      541 agaggatttt tatattcctg aaggagaaac tttttcggag agtaagacgg gtaaagaatt
      601 cagcgaaacg tcgaatgaag cgcaatcgtt gatgaaacat ggaatcaatt ttgacaagtt
      661 agaagaacaa gattttacca aatatctttc ttatatgaca cgtcactatg cgttagaaca
      721 aaagaaagta ctgacgcata gtgaacaaaa agaataccaa cgcttgaacc aagcaataaa
      781 aaaatatcaa acagagccac acaaaaaagc ttcaggaaat cttcctggag ttttttctat
      841 ggcttcagac gaagtgaaag gaagtgatct gaaggagatc aagcaaacct tccaaagagg
      901 acaacaaaaa ggctcaatca tttatcaaga tgttgtctct catgatcatt cgtatcttga
      961 aaaaatagga ttgtacgatc ccaaaacaga tactttagat gaagaaggtt taaaagcagc
     1021 tggaagaaaa atgatggaga cattgttcga gaaagagcaa ctgaatgaaa caggctactg
     1081 gatggcaacg attcaccgaa ataccaaaca tatccatatt cattttgcag ttgttgaaaa
     1141 aaaaaatacg cgtaaaccat acactgagat tgaaaatggt gtgagttata tcgtaccgaa
     1201 agggaagaga aggcaagcaa cattagacga aatgaagact aattatgtac atgagttaga
     1261 acgtttttct tatgaaaaaa atcgaatggg tatggagaaa cgaaatttat tgacacggaa
     1321 aagtgatttg cgtaacacct taaccaaagt agtgaaagag ccagtacgtt atgatcgaca
     1381 cgcaatgagc ttattaactg aagtatataa aaacttacct aaaaagcgtt cggaatggca
     1441 ttacggggat gaaaatcgaa ccaaactttc accatacaca agagagaaac tcgatgagtt
     1501 aacccaatat gtgttaacaa aagaacctgg atatcaagaa tatatacaga tttcaaaagc
     1561 attgaaagaa gaagcaaaaa gtctttatgg ggaaagcaag agagaatcaa aggatgcaga
     1621 aaaaaatgct ctctttgatt taaaaaagcg cacaggaaat gccattctaa cagaactgaa
     1681 gaaacaggat agtcaaataa aacctttaat agaaaggatg gatcagatgc aatctattta
     1741 caatgaacga tcctttcgaa cacttaaaca gttgccaacg aatgaataca aatatagtta
     1801 cgaagaattc atgaagcgca ggaaacaatt ccaacaaaga gttgtcacgc aacggagttt
     1861 gcgtcaatta aatcgaacga ttaatgccca tgaagaaaaa taccacgcaa gtaaagcata
     1921 cgaagagcaa caacgaagaa ttcaaatgga acaagaaaga cagtaggagg aatagcaatg
     1981 aaacaatatt tagatcaatg gaaagttata gaaggatcat taagagaaga acgaatcgaa
     2041 caacttcccg attgtttaga aaaagagcat ttgtttcaga taagagaaat gttgataaac
     2101 gaacagtttg atccaaatca atttttagtc gttgagtacc cagctactgg tgtgtactgt
     2161 tgtaatcatg tgaagggtga aaaatatttt atcattcaag aatatgaggg caagttagcg
     2221 ccttattata ctacttggga aatgaatgaa gaggggatca ataacttccc gtgtaaaagc
     2281 attgaagagt ctatttcact cacagaatgt tagttgttaa gtgaataaaa gaagtaaaag
     2341 agtcctgaac gtgattgtcc aggactcttt tttttcactg aattgttttc cgccaattca
     2401 ttgagtggtg cgatttccgt ctacattata tcaaaagaaa ggaggaagaa gaaatggctt
     2461 attcaacgat tggagttgtt gttgaaaaaa gcagagacaa tcttgttttt gtgacagaaa
     2521 ttcaaacagg cagagctttt gtagtcacag acaaagcggc aaaagcatat caaagtggcg
     2581 atattttaac gttaaatctg actacgaaga cgttcgtcga tgcggcagaa gattatccat
     2641 t
//
LOCUS       DANKKG010000216         2621 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000216 DANKKG010000000
VERSION     DANKKG010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.218"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(168..716)
                     /locus_tag="PW004_002747"
     CDS             complement(168..716)
                     /locus_tag="PW004_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727670.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(717..1574)
                     /locus_tag="PW004_002748"
     CDS             complement(717..1574)
                     /locus_tag="PW004_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDL2727671.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(1860..2147)
                     /locus_tag="PW004_002749"
     CDS             complement(1860..2147)
                     /locus_tag="PW004_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL2727672.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            complement(2137..2466)
                     /locus_tag="PW004_002750"
     CDS             complement(2137..2466)
                     /locus_tag="PW004_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDL2727673.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
BASE COUNT          772 a          530 c          343 g          976 t
ORIGIN      
        1 cacaatatct cattaaaaca caccctaaaa tcataaccgt tcctatgctc attttttgga
       61 ttttttaatc acgtaaccag aaaaataaat cccctctatt tcacgcaaat caaagaagaa
      121 ctaagtaact accgtttaaa ggtagttact tagttcttct ttgattatta tctaacttta
      181 ttattcattc cccacccttt gcgcagtgag tcttcggtat cttgttttat ctgactggta
      241 gttcttatta tttctccacg atcatctaat tctggaacaa ttggatattt ttctctgaac
      301 aagtctagtt ctttcaccac gtcattaaac aaatcttgct tatcttcttg cgtaagattc
      361 tgtttaatat actcatctaa agcttttcta agcaattcat ctcttgtcat attagtctca
      421 tacttttcta ctaaaactct ggaagtcgca tttaacaaag tcgacagttt taaaggatgc
      481 ttggtggaat aaaccttatc aaactttctg attggtgcgc caactttttt tgttcttcct
      541 ctactctgtc tttcaacatt ggaggaagtt tttatttctt gaacttgatg cacttctaat
      601 tgcttatttt cagatggtgt aattgcttgc tgctttttct ttgcttctgc ttcttttttt
      661 attttggcga gtcctttgtt gttcccaaaa atatcaagtc ctttgatgtc agccaattac
      721 tccacctccg cataccaatt ctcgtgttct tccaattctt tcaaaatact gataaataat
      781 tcgtggcttt tgcgatccca ttgttttaag agacctttct ttgtcttctc catcgtaata
      841 cctgatccgt cgtatcgttt taagcgcgca tttttgagta caacattttt aatcaaatgt
      901 tccccataaa tctcttgggc ttgtttatac atttcctgat ctactgcgtc accgctttcc
      961 aacatgaaag gaataacacc agcaacatat aaatttgtat caaattcttt cacaaaaaag
     1021 tccatatact caagatattg catcgcattt ctcatcgatt ttacttgggt ctgtaagacg
     1081 acgattgccc agtccgcaat gtacatcgcg gatttactat atacagaaat agaaggtggg
     1141 acatctaaat agatgacgtc aaactcatca aataatggtt ctagcttttc tttcatatat
     1201 ttgaattggg ataatgaatc attatattgg tctttgatcc aatcatcata catttcaaag
     1261 gacatgtctg ctggaagtat atagaggtta ttgattactt tatagacagc ctctttaggt
     1321 ggaagattgt ttatcaacac gtcagtcaca gtattttcgt aaacaatcgg ttcaccttct
     1381 ccgcctcctt cttcgttagt gaagttcata tgcgttaagt tcatcacatc agaagtattt
     1441 ccttgcatat ccaagtcaat tatcaaggtt ctttttcctt cattaactgc tgattcaaac
     1501 cctaacattt gcgcgcacgt acttttacct acgccacctt taaaattacc gattacatat
     1561 ttcttcgcta ccacgttcct acctcttttc ttttagcaat attcatttga tactattata
     1621 acatatttat ttttagttac taccaaaaaa aagatatata tgtactacct tataattaat
     1681 aagtaaaagg tagtacataa ataaggaaaa gctgaaatat attgatttat agctttcctc
     1741 aaagtaatga cgttatttaa tttatcgaga agcagagaat actcgcgact ttagtcatgt
     1801 agacgaatca gattttcgat aaaaaagaag ccgtccttct taagaacgac aacaaatcac
     1861 tagattaatt ttttcaactg ttcataaaag ttttcatgcg ttccacataa caatactgga
     1921 ataaccttgt tttctataat ctcgtaagct actctatatg ttgtgcccgc atatttgaac
     1981 ccactagacc aaatacctga taaatcccca gttttttgtt cacccttata tggatcaatc
     2041 gcaatttcat cataaattaa attcaaaaat ttttctttca atggcttctc ttttagcttt
     2101 tttaaatact taatgacatg ttttgaagga acgatttcat aagccaattt ctgttgccaa
     2161 ctcctcttta gtcattactt tagaattagt aagtgtatct tcagcaatat ctctaaagga
     2221 agaatgcatt ttgttttttc tattcttaaa ttctacaaga atctcatctt tatcgtagcc
     2281 ttctgcaatt atatcagaca aaatatcttc gctaaaatca taaaattctt tttgtggttc
     2341 aacaaattca taaagaatac cattctctac caactttgct tcaacttcga ctccattagg
     2401 aacattaaaa tctttaggta ctgtcaacat gattgaattg ccttgttttc ttgtttttgt
     2461 aatcatttaa atccctcatt tctaacagta tgtaattact tagtaaatac attataccac
     2521 gagataatgt atttacaagt aattactgtg actcgtaatg cgtccttgct gaataaattt
     2581 caaccacgtg ttcatcttca tctaccttat aaacaacccg g
//
LOCUS       DANKKG010000217         2617 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000217 DANKKG010000000
VERSION     DANKKG010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.219"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(<1..187)
                     /locus_tag="PW004_002751"
     CDS             complement(<1..187)
                     /locus_tag="PW004_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727674.1"
                     /translation="MASWLIEEIENERRKIMDAGITVMLDKQQTNQLKNYVFEMTKEA
                     IDQARIDTGLERPFLKGK"
     gene            complement(199..399)
                     /locus_tag="PW004_002752"
     CDS             complement(199..399)
                     /locus_tag="PW004_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2727675.1"
                     /translation="MITLNDQFIRSLRRHRADLILTKNDAAKLIGINRKTYVKIENGS
                     KESIRASTYQKLVNWLLNDLKI"
     gene            522..1124
                     /locus_tag="PW004_002753"
     CDS             522..1124
                     /locus_tag="PW004_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2727676.1"
                     /translation="MEFTIGGVTLNRIKFYIDSSKKTIKQISTETGINYTTLTNYYRG
                     ERNPRNTETWNILADYFHVPVSYLMGLDETNSIEQPNLDRIKTARLEKKDKIVKNIAS
                     LDSDYFTELSNLYFSMTSLLEVKLQEDDSDFLFLKSILEDSFEYTILFAKQKKFINKA
                     LNLNIDERKELESELFGNLTQVQVNNLKREITARLISKLQ"
     gene            1179..2345
                     /locus_tag="PW004_002754"
     CDS             1179..2345
                     /locus_tag="PW004_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2727677.1"
                     /translation="MSIQKYTTKSGARYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDTGYHKPSNDLFITVYEEWLPSYRNRVQETTYQRTKDLFRLHILPKFG
                     NKSISKITPVHCQNAVNEWALKFKNIKQLKSYSSQIFEYAIFAELLHRNPMANTNLPK
                     REKKENENYFSLEELKAFWDILNKEEPLKHILIFQLLITTGIRKGELAALHWSDIYFE
                     EQLMYVRKSYATIRNEEKNARRKTVRIQKNTKNISSERILPIDAQTIEMLKIWKKEQA
                     RELLQFGINTNTKNQLIFTYVNADGDINQPLHADYSNNILKRLEKKYKFKHVTIHGFR
                     HTHATLLLEGGASIKETQDRLGHKNAETTLNTYSHVTEKAQRNALEKFIAYTGL"
BASE COUNT          934 a          430 c          398 g          855 t
ORIGIN      
        1 cttttccttt tagaaatggt ctttctaatc cggtatcaat tcttgcttga tcaatagcct
       61 cttttgtcat ttcaaaaaca taatttttca attgattcgt ttgttgttta tctaacatta
      121 ctgtaattcc tgcatccatt atttttctcc tctcattttc tatttcttca atcagccaac
      181 tcgccaagtt gtatttattt agattttcaa atcattaagt aaccaattta ccaacttttg
      241 ataagtagat gctctaatac tttctttgct cccattttcg attttgacat atgttttccg
      301 atttatacct atcaattttg ctgcatcgtt tttagtaagt ataagatcgg ctctatgtct
      361 tcttaagctt ctaatgaatt gatcattaag agttatcatt cagcgcctcc tttcaaatta
      421 ttcactttgt ggtttttatc ttaataagca cattttaaac cacaaaacga gtaatatcaa
      481 ttaaaatatt actcaatttg tggttttcat gctataatag tttggaattt actatagggg
      541 gcgttacttt gaatagaata aaattttata ttgattccag taaaaagacc attaaacaaa
      601 ttagtacaga aactggtatt aactatacaa ctcttacgaa ctattaccgt ggtgagcgaa
      661 atcctagaaa tactgaaaca tggaatatcc ttgctgatta tttccatgtt cctgtaagtt
      721 atttgatggg attagacgaa acaaattcca tagaacaacc taatcttgat agaataaaaa
      781 ctgctcgttt agaaaaaaag gacaaaatag tcaagaatat tgccagtctt gactcagatt
      841 attttactga attatctaat ctatactttt ccatgacttc tttacttgaa gtcaaacttc
      901 aagaagatga ctccgacttt ctttttttaa aaagtattct agaggactcc tttgaataca
      961 cgattctatt tgccaaacaa aagaagttta ttaataaagc tttaaactta aatatcgatg
     1021 aaagaaaaga gttagaatct gagttatttg gcaacttaac ccaagtacag gttaacaatc
     1081 taaaaagaga gataacagct cgattaatat caaaacttca ataaaactaa ttattcaatc
     1141 agccaactcg ccaagttatc agattggaga gattagttat gtcaatacaa aagtacacaa
     1201 caaaaagcgg tgcacgatat agtgtccgct tgtatgttcg taaagatgaa aatggaaagc
     1261 aagaatacta tgtaaaaaaa gggtttaaaa ctgaaaaaga agccaagtta cacgaagcaa
     1321 gaaaaaaggt cgagattgaa gataccggct atcataagcc ttctaatgac ttgtttataa
     1381 cagtgtatga agaatggttg ccttcttata ggaatcgtgt acaagaaacg acatatcaac
     1441 gtacaaaaga ccttttccgc ttgcatatac taccaaaatt cgggaataag tccatttcta
     1501 agattactcc tgttcactgt cagaatgcag tcaatgaatg ggctttaaaa ttcaagaata
     1561 tcaaacagct taagtcttac agctctcaaa tatttgagta tgccattttt gcagagctat
     1621 tacaccgaaa tcctatggca aatacaaatc taccaaagcg ggaaaagaaa gaaaatgaaa
     1681 attacttttc attagaagaa ctaaaagctt tttgggatat tctcaataaa gaagagcctt
     1741 taaaacatat tttaatcttc cagcttttga ttactactgg tatacgtaaa ggagagctag
     1801 ctgctcttca ttggtctgac atttattttg aagaacagct aatgtatgtt agaaagagct
     1861 atgcaactat tcgaaatgaa gagaagaatg caagaagaaa aactgtcagg attcaaaaaa
     1921 atacaaaaaa tatatcttcc gaacgaatat taccaattga tgcacaaact attgaaatgt
     1981 taaaaatctg gaaaaaagaa caagctcgag agcttttgca gtttggcata aatacaaata
     2041 caaaaaatca acttatcttt acctatgtaa atgcagacgg tgacattaat caacctttgc
     2101 atgcggacta ttcaaataat atattgaaac gtttagagaa gaaatataaa tttaagcacg
     2161 ttactattca tggattccgg cacactcacg ctactcttct actagaaggt ggagcaagta
     2221 tcaaagaaac acaagatcga ttgggtcata aaaatgctga aactaccctt aacacttatt
     2281 ctcatgttac tgaaaaagct caacgaaatg cgttagaaaa attcattgct tacactggtt
     2341 tataatattg tcattacctt tctcattacc agaacgaaaa aaagacacct tctcgcaatg
     2401 agaaagtgtc tgaaatcgtt ggtataacaa cgattaacgt tttgaaaact gtgaagcttt
     2461 acgtgctttc ttaagacctg gttttttctt cgtatcattg aataagtttg aaaagccttg
     2521 atttataaag gtttttccgt aaatatattt tgttatattt gattaaattt gaaatgtttg
     2581 tgttctaaaa tgtgttctaa tttaaaggca gtatatt
//
LOCUS       DANKKG010000218         2556 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000218 DANKKG010000000
VERSION     DANKKG010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.220"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(302..868)
                     /locus_tag="PW004_002755"
     CDS             complement(302..868)
                     /locus_tag="PW004_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDL2727678.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(868..1824)
                     /locus_tag="PW004_002756"
     CDS             complement(868..1824)
                     /locus_tag="PW004_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HDL2727679.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(1824..2480)
                     /locus_tag="PW004_002757"
     CDS             complement(1824..2480)
                     /locus_tag="PW004_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDL2727680.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
BASE COUNT          770 a          528 c          471 g          787 t
ORIGIN      
        1 cttttaaaaa tgcttatata tcaatgtttt tgccattctt ttatatatct gttatatgtc
       61 aaaaaaatgt gttcaatttc aatttgtatt tgaataaaga ggttgtgaaa aagctgtcct
      121 gcatcaagta ataagaacag ccaatcaaaa acagcttctc atgtttttga tttggttgga
      181 cttattaccg cagatataag cttttgagca ccgtgaataa agaggttatg aaatcaacgc
      241 tctaacctga gtattagaga aaaaaacgca aaacatatca tttatgtttt gcgtttttta
      301 tttatctttt tttctcaata caatataaaa taggcggctg gttcttttgg ttgataaacc
      361 gataattcag cacattatat gtttcctgtg gcagtttgga acaaaaagaa tcaaccatat
      421 ccagttcttt ttctccacct tcatgaccat agtagacgac aaggatcaat cgtccgcgag
      481 gcacaagtcg tttcaatatt tcttccatag ctgtgcgtgt agtctctggc atagtgatga
      541 ttgctttatc actttttggg agatacccta gattgaaaat ccctgctttg agattttgtt
      601 gttcagctat cacttctcct aacgtttcgt ggccttgtag aaacaatgag acacgttctt
      661 ctaactgacg ctgttctagc ctttcacgag tagcatcgat tgcttgttgc tggatatcaa
      721 aactatagac atgaccagtt tttccgatgt gttctgctag aaaaaccgta tcgtggccat
      781 tgcccatcgt tgcatctacg acgtgatccc ctggttgcaa gatttcttgc agtaatgtat
      841 ggctgaaatg caaagcagtt tgcaacatca gacgctcacc tttttccgta catcatattt
      901 tccttggaag ctatctctgc gaagaagttc ttcatcaatt gcattcaaga cttcccattt
      961 tttcaagctc cacattggtc caatcaagga atcccacggc gcatcccctg tcaaacgatg
     1021 gatgatgatc tcttttggta tcatttccag ctggtcgcaa atgacattga catagtcagg
     1081 acggctcatt aattgaaggc gaccttcatg atagtcccgc aacattctcg ttttgcgcat
     1141 cagatgcatc aaatggagct tgattccttg gatatctgaa tctaaaatgg tgcgtctgac
     1201 attttctttc atcatttcta aactttcgcc tggtaatccg ttgatcaaat gcgtacaaac
     1261 acggatatta tgtttgcgta atttagccac gccatctaaa taagtttgat aatcatgtgc
     1321 tcggttgatc agttttgaag tttcttcaaa agttgtttgc aatccaagtt ctacccataa
     1381 atacatccgc tcgttcagtt cagcaaggta atcaactact tcatctggca gacaatccgg
     1441 tcgtgtaccg actgataatc ctacgacacc tggtaaattg actacttgct caaatcgttc
     1501 tttgattatt tcaactggcg catgggtgtt tgtaaaattt tggaaataaa cgatgtattg
     1561 ttggacatga ggccattttt gatgcatcat gtcgatttct ttctggaatt ggattggaag
     1621 agggtcttca ggtgcaacga tcatgtctcc cgaaccagat acactgcaaa aagtacaacc
     1681 gccatgggca acagtcccat ctcgattcgg acagtcaaat cctccatcga tcggcacctt
     1741 gaaaattttc ccgccaaact catttcgtaa agcgtaattc caagaatgat atcttttatt
     1801 tccttctgaa taaggaaaag tcattaaaag tccttctttc tgtaagagtt atctatttgt
     1861 tttctttttt ctctatacac tggataagtc aggcacagcc aagaaagact gagtaagtag
     1921 ccgccgatga tatcagtagg aaaatgaacg cccagataaa tgcggcttat cccgacactt
     1981 actataataa acatcatcag caattgaagc aatgtgcgta aggttctttt ttgaacaaaa
     2041 gctggaagca gaaatagtaa agaaccgtaa agaatcatac tgcctgctgc atgtccacta
     2101 gggaaactgt aacttgtttc aactactaag tgcgccaatg aaggacgctc tcgcatgaaa
     2161 aatagtttga tcaccgggtt gatgattcca gcaattacaa taaatccaga aacgaaccaa
     2221 aatgtttctg cccttttttt cttataccac atccataaag ccgcacaagc aaataaaatg
     2281 atgacggtaa gaggattacc gaatttagtc ataaagagtt ggtagctgtt cagatcagga
     2341 taagaagctc tgacaatgcc tgtaaaccac tgatcgaaac cagaaagcca cgacagatgg
     2401 aactttacga tccagcccaa aatgaaaaac aggacaagaa agaagctacc agccacctga
     2461 aatgatgttt tatatttcat acaattcctc ctttgaagca atgacttatt atagcatttt
     2521 tggtccagcg aaaaaaacgg taaagagata aaacca
//
LOCUS       DANKKG010000219         2552 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000219 DANKKG010000000
VERSION     DANKKG010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.221"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..997
                     /locus_tag="PW004_002758"
     CDS             <1..997
                     /locus_tag="PW004_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDL2727681.1"
                     /translation="KTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFYMQQE
                     LSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNVMGTQ
                     NVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIFSCVR
                     FGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSKFGET
                     YVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEIERSY
                     ENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVTW"
     gene            1009..2013
                     /gene="neuB"
                     /locus_tag="PW004_002759"
     CDS             1009..2013
                     /gene="neuB"
                     /locus_tag="PW004_002759"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HDL2727682.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2010..>2552
                     /gene="neuC"
                     /locus_tag="PW004_002760"
     CDS             2010..>2552
                     /gene="neuC"
                     /locus_tag="PW004_002760"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HDL2727683.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILN"
BASE COUNT          932 a          332 c          508 g          780 t
ORIGIN      
        1 aaaaacaata ttagtaactg gcggaactgg ttccattgga agtgaaattg ttcgtcagct
       61 tttaaaatat gaaccaaaag ctatcagaat ttatagtaga ggtgaagaaa aacaatttta
      121 tatgcaacaa gagctctcaa atcacactaa tttgagattt ttaattggtg atatcagaga
      181 ctctcaaagg attgattatg catgtcgtga tgtggatatt atttttcatg ctgccgcaat
      241 gaaacacgtt ccagcaagtg aatataatcc tatggaagct gtaaaaacaa atgtgatggg
      301 aactcaaaat gtaatagatg cagcgatcaa aaataatgta aaacattttg taggtatcag
      361 tacagataaa gtagtgagtc ctactaacac catgggtgcg acaaagttat tgagtgaaaa
      421 attaataata gctgccaatc agtataaagg aaaaacagaa acaatatttt cttgtgtaag
      481 gtttggaaat gtcatggggt caagtggatc tgttattcct ctttttagta aacaaataaa
      541 aaacaataaa gatattactt tgacagatgt tgatatgaca cgatttatga tgtcaatacc
      601 ggatgctgca agattagttc tgtctgctgc tatgaattca aaatttggag aaacatatgt
      661 cttaaaaatg ccggcaatca atattttaac tttagctcaa tcattaataa aaatatataa
      721 tgatacacat gatgaaaaat atagaggtga aataaaagtt gtaggtatca ggccaggaga
      781 aaaattatat gaagagctaa tgactacaga cgaaattgaa cgctcatatg aaaatgataa
      841 aatgtatatc attccaccac actatcatgc tactgacgaa ctgtacaaag attatgaaaa
      901 aattaaaatc actgcatact cttcagatgc aacagaatta atgagtggac aggaatttat
      961 tagaatcgca aaagagaata atgtcgtgac ttggtaagga gttttatgat gaaatcagaa
     1021 gtatatgtaa ttgcagaaat tggtgtaaac cataatggaa gtctagattt agctttagaa
     1081 agtattgata aagctaaaga atgtggagct aacgcggtta aatttcaaac atttaaaacg
     1141 aataagctag tttccaaaaa ggctccaatg gcaaaatatc agaaggataa tcttaaagag
     1201 gacagttctc aatttgaaat gctaaaaaaa ttagaattat ctcaatctga ttttattaga
     1261 ataaaagatt attgtataca aaataaaatt gattttttgt ctactccttt tgatgaagaa
     1321 agtgcagact ttctaaaaca aattggtgta caaggattta aaattggctc aggagattta
     1381 acaaatttac cttttcttag aaaaatagat atgtatgggt tgccagtttt attatctaca
     1441 ggtatggctg atattcatga ggtcagtgag gctgtagatt gtttcataga tagtccagtt
     1501 actattttac attgtacttc aaattatccg gctagttctg aagatattaa tttattggcg
     1561 attaacacat taaaagaaat gttcgggata cccgttgggt actccgatca ttcattggga
     1621 tatgatgttg gtatttgtgc tgttgcgatg ggtgctaagg taattgagaa acattttacc
     1681 ttggataagg aactacctgg tccagatcat aaagcttctt tagatcctca ggaatttgca
     1741 gattttgtaa agcatattag aaacactgaa atatttttag gtgatggtgt taagcgagca
     1801 atgccttctg aacttagtac aaaagaagta tcaagaaaaa gcctagtggt cactgagaat
     1861 ttaaatgtgg gagatattct tacagagaaa tctcttacaa taaaacgtcc tggaaatgga
     1921 atagctccta agtttttata tcaatatatt ggcaaaaaag ttaaaagacc aataagtgca
     1981 gatagtgttc tgcttgagga agatgttgaa tgaaaaaaat acttatagta agtggaacac
     2041 gagctgatta tggaatatat aaacctgttg ctgagaattt actacaagat aattgggatg
     2101 tatcatttgt tgtttctgga atgcatttat cccataaata tggatttact aaagaattga
     2161 tcttagctga cggatttaga attagtggag aagttcaaag tttgttttta gagaacaata
     2221 agggaaacat ggctcggtct gtggcattag agataaatgg tttcacaaat atatttgaac
     2281 aaactagccc tgattttgta ttattactag gggatagagg agaaatgttg gcagctgcga
     2341 tagcttgttt gtatcttgga atcaaaacag cacatattca cggaggcgaa gtgtctggta
     2401 ctgtagatga atcaataaga catgccataa cgaaaatagc atctgttcat tttgctgcta
     2461 cggaaaaaag caaagagcgt ttaataaaaa tgggggaaga ttcttggaga gtttttcccg
     2521 tcggagctcc tagaatagat actattttaa at
//
LOCUS       DANKKG010000220         2522 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000220 DANKKG010000000
VERSION     DANKKG010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.222"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            184..1149
                     /locus_tag="PW004_002761"
     CDS             184..1149
                     /locus_tag="PW004_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HDL2727684.1"
                     /translation="MDKTGTITVGKPQVTDIISIGRISENEILRIAAGLEDSSEHPLA
                     LAVINEAKDKKITPAVAKNFTAISGKGVQALIDGKQAFIGNDRLSDDFNMTDDLKIKM
                     TSLQAQAKTVVLVGYDGQIIALIGIQDAPKSSSKAAIRAMQKSGFHTVMLTGDNRLVA
                     QAIADDIGIDEVIADVMPGDKAQHIRKLQEKGAVAFVGDGINDAPALSTATVGIAMGS
                     GSDIAIESGGIVLVKNDLMDVVTSLVLARKTYSRILINLFWAFIYNVIGIPVAAGIFS
                     ALGFTLSPELAGLAMALSSITVVLSSLLLNYVRLPKNSETLIGNS"
     gene            1429..2522
                     /locus_tag="PW004_002762"
                     /pseudo
     CDS             1429..2522
                     /locus_tag="PW004_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249826.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          823 a          452 c          496 g          751 t
ORIGIN      
        1 tagtaaccaa cagcgacaat aatgacgtct tttttgaatt gtttgccttt aaaatgattc
       61 attactctgt cctctctgtc ttttttctca attttacact aaaatagatt ttttggaaaa
      121 ctttgcaaca gaaccaaaaa tgctgaggtt cttgaagcga cccacgatat aaaaaccgtc
      181 gtcatggata agactggaac aattactgtt ggcaaaccac aagtgaccga tattatctct
      241 atcggaagaa ttagtgagaa cgagattctg cgaatcgctg ctggacttga ggattcatca
      301 gaacacccat tagctttagc agtaattaat gaagcaaagg acaagaaaat tactcctgcc
      361 gtagctaaaa atttcactgc tatttctggt aaaggggtac aagctttgat tgatggtaag
      421 caggctttta ttggtaatga tcgtttatcc gatgacttta acatgacaga tgatcttaag
      481 attaaaatga catctttaca ggcgcaggcg aaaactgtgg tattagttgg ttacgatggt
      541 caaataattg ctttaattgg gattcaagat gcacctaagt ccagctctaa agctgctatc
      601 agggcaatgc aaaaatcagg atttcacact gtgatgctaa ctggggacaa tcgtttggtc
      661 gcacaagcca tagcagatga tattgggatt gacgaggtca tagcagatgt tatgcctggg
      721 gacaaagcac aacatattag aaagttgcaa gaaaaaggag cagtcgcctt tgtaggtgat
      781 ggaatcaatg atgcccctgc attatccacg gcaacagtag gtattgctat gggatcgggg
      841 agtgatattg caattgaatc tggaggtatt gtactagtca aaaatgattt gatggatgtt
      901 gtaacttctt tagtattagc acgaaaaaca tatagtcgga ttttgattaa cttattttgg
      961 gctttcatat ataacgtgat aggtattcca gtcgcagcgg gtatattctc agcacttgga
     1021 tttacactat ctccagagtt agctggtctt gcaatggcac ttagctcaat tactgttgtt
     1081 ttgagctcac tattattgaa ctatgtgcgc ttgccaaaaa atagtgagac acttataggt
     1141 aatagttagt atttgtaata tacaaaagtg acgcttttaa ttataatagt aattttcctt
     1201 tttcaaaatt tgaatcagag tcttaaatac taggcataca ataagagaac caaaaatcag
     1261 aactactgga tgtagttctg atttttgatt ctacaatatt tagtgttggt ggaaaatcta
     1321 cagattttct actgacactt tttttgtaaa aaaagataga aaaagagata gcctaactgg
     1381 tggaatttag tcgtctaaaa caaaaccata ccaagaaagg atatctccat gtatcattgt
     1441 actagaaaat tgttaggttt aacagatgaa aatttattct tcgaagaaga atggttagag
     1501 acagtagaag aagatggttt tcgcaccaat cttatccatg ataaacttag ctatatacta
     1561 agccattgcc gtaaatgtgg catcaaaaat gaaggacaaa tcatcaagaa tggctcacat
     1621 aaaacaaaag tccaactttt gccttaccga gcaaccaaaa cagaattacg cttggtacgt
     1681 acgcgctttt actgtaaaga atgtcagtcg acattcaacg ctcagaccaa tttggtagat
     1741 gaaaactgct atctctcaaa ggaattaaag gtacaaattg ccttggaact agcgaagaat
     1801 acaataaaaa aagaaattgc caatcgctgt tttgtatcag acgtaatcgt tttgcgtgtc
     1861 ctgcacacct gtttaaaaac gtatcatcct cgttttgata cgttaccttc ggtcctttgt
     1921 tttgatgagt acaaatcgat gaagtcctgc agtggaaaaa atgagttttg tttttatgaa
     1981 tgggcagacc cagcaattaa tcggtgtatt ggagaatcgt cgccttactt tcttaaaacc
     2041 atatttcctt aatttcactc gaaaagcacg agcgaacgta aaatacgtcg tgatggatat
     2101 gaatgcccct tattttgaac ttgtaaaagc ggtcttccca aacgccaaaa tcgtgaccga
     2161 tcgctttcac attgtccaac aaatcactcg tacgttaaat cagttgcgaa tcaaaacaat
     2221 gaacaggttt caaaaaacgg aaccgacaaa gtattgacga ttgaagcgat tctggaaatt
     2281 actccttacg catgcctatg atctggatag ttctgattat caatatgatc gatcctttcg
     2341 cagaccaatg acgcaaaaag cgatggtcga tgagcttttg agttacgatg agcaattaac
     2401 cagggcttac gagacttgct agctcctcct ctatcacttt aaacacaagg acaaccaaag
     2461 ttttttcgat acgatcaaca gcttagatca atgccttcca caatggtttt gtaagaagct
     2521 ga
//
LOCUS       DANKKG010000221         2398 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000221 DANKKG010000000
VERSION     DANKKG010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.224"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            207..2069
                     /locus_tag="PW004_002763"
     CDS             207..2069
                     /locus_tag="PW004_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL2727685.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2398
                     /locus_tag="PW004_002764"
     CDS             2069..>2398
                     /locus_tag="PW004_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL2727686.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          865 a          353 c          466 g          714 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataat
//
LOCUS       DANKKG010000222         2376 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000222 DANKKG010000000
VERSION     DANKKG010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.226"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            211..1824
                     /locus_tag="PW004_002765"
     CDS             211..1824
                     /locus_tag="PW004_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL2727687.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT          747 a          421 c          453 g          755 t
ORIGIN      
        1 tcatttttct tcagctttta aaaaatatac agggttatcc ccttcaaagt attatctatc
       61 cttaaaatag aaaaaaattt ttaggtttta tccacaaatt atttctctat attttctgtc
      121 tattcaaaat aaacaataat tttccggcac aattatttgc tgttttttac cataacatag
      181 tacgatcaac gtagaaaagg agatgttttt atgaaagttg ttgtcattgg cggtgtagct
      241 ggcggtcctt catttgccac tcgtttccgt cgattgaatg aagcgcacga aatcattatc
      301 tatgaacgcg gagaaaatat ttcttatgca agttgtgctt tgccttatta tttaggaggt
      361 gtgatcactg accgtgactc gctgatcgaa cgtacaccag aaatattgaa aacaaaaaac
      421 aacatcgatg tctttactaa acacgaagta acagcaatcg atccttctac taagcgatta
      481 acagttaaag acctatccac aaatgaagaa ataaaaacag attacgataa gttgatcatc
      541 tcttctggtg ctagaccaga ttatccggat attcccggag tttttgaagc agaaaacggc
      601 tttgtcctcc gtagtgtgac ggatgcggat cgaatcaaat cgttccttga agaaaaaaat
      661 ccacaacatg tcgtcattct tggtgcaggt gttatgggtc tggaattagc tgagaatttc
      721 aagcatcgcg gcttagacgt gactttaatc gatcaattgc cacaagtcgc tttcccttat
      781 gatccagaaa ttgctaattt agtttatgac aaattgctga aagaaggatt agccgttcat
      841 ttagaaacaa gagttactga gatccgtgat aaaggtcgag aaatagtatt atcagatggc
      901 tccgtccttt ctgctgatat gctaattttt gctgttggtg tttctccgaa gaatgaagtg
      961 gtgaaagcaa ccggcataca attatcagat acaggtcaga tcattgttga tgaccagtta
     1021 caaaccaatc tgccggacat ctatgcgatt ggcgatatta tcgaaacgac tagtgtagtg
     1081 actggtcagc cgatccaaag tatgctttcc agtgcggcca atcgtcaagg acatatgctg
     1141 gcagatattt taaatggtac gcctatgcgc tatcgcggat atattggtgc aggtgtcgca
     1201 aaaatctttg atcatacagc aagttatgcg ggaatgacag aacatgaatt aaaagcatca
     1261 ggcataacaa attataaaac tgtttttatc actccctttg accatgccta tttctatcca
     1321 gaagctacaa gattaaatct aaagctgatt tttgatgcag atagcggtcg tattttaggt
     1381 ggacaagcat ttggagaaaa aggtgtcgat aaacggatgg gagaactttc tgtagcaatc
     1441 accggaaact tgacagtctt tgatttgcct gatttggagt tgccttactc tccaccgtat
     1501 tctactaccc gtgacccgtt gaatatagct ggttatgtcg caatcaatca aatgacgaat
     1561 atcgtagaaa cgatcaaagc aagtgatata cccgaaaacg atttgaaaga agcgttcttt
     1621 ttagatatac gtgaacctaa taaagcacct tctggcagta tctcggcaac aaaaaatatt
     1681 ccaatgaatg agttacgaga tcgaatcaat gaaatcccaa aagataaaaa agtttacatt
     1741 actttcagaa gaggattgaa tacttatact tctgcccgaa tcttggcagg tttgggtatc
     1801 aaagcgattt tgattgaaga ataaaaaaac gaccgcggtg ggtagccgcg gtctaggagt
     1861 tatttttact tgaggagtaa aaatgaaaaa gtatataggg ttgttggttg gtatgtatat
     1921 agaatagaag ataaatgtga agaaattgtg cattatttct tcacatttat taggttatag
     1981 tttaagaaag tttaaacatt gcgcaattta attattttag ctgtttcttt tcataaaaaa
     2041 acatgttgtg actctgagac aaatcatagt ccatagtgat gtctctagag taactacgcc
     2101 taatattacg aggcatcata ttaatctatc agcactagta cctaaactag catccctttc
     2161 gaatattttt tcttatataa taaagtttgc tttcactcct ctaatctcac caaatcgact
     2221 ggcttgcgtt gtgtgatact aggaactgat tggtttagaa cgacttttct attgtaatca
     2281 tatttttcgc catcctcgtt gtcttctaat ccttcaatga atgatcccta atgtgtgtat
     2341 taaataattt aatttttttg ttcctattta tttaac
//
LOCUS       DANKKG010000223         2367 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000223 DANKKG010000000
VERSION     DANKKG010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.227"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            1..269
                     /locus_tag="PW004_002766"
                     /pseudo
     CDS             1..269
                     /locus_tag="PW004_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751573.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
     gene            808..1008
                     /locus_tag="PW004_002767"
                     /pseudo
     CDS             808..1008
                     /locus_tag="PW004_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1495..1674
                     /locus_tag="PW004_002768"
     CDS             1495..1674
                     /locus_tag="PW004_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727688.1"
                     /translation="MTELTADMSDTWLQEYLFPEKQTTARGYLPVDWEYVHKELMKKN
                     MTLKLLHREYGVHGT"
     gene            complement(1720..2325)
                     /locus_tag="PW004_002769"
     CDS             complement(1720..2325)
                     /locus_tag="PW004_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2727689.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
BASE COUNT          783 a          393 c          424 g          767 t
ORIGIN      
        1 aatctatatt ttgatataca aattacagct tcagatacta agttgcataa accttttccg
       61 gaccccatac aaaaggcaat tgatgaattg ggcataaaaa ataagaaacg atttatattg
      121 gtgattcatt atatgacatg caaagtgcga aagcttgtgg gatagcattt ggtttagcta
      181 aatggggagc caaaaatata gaaaattttc aaagtgtaga tataattatt gattctcctc
      241 aagaaatact tgatttgtta gaaaaataga caaagagcgt gagataagga tagggtctca
      301 cgctctttgt ttttcgccat ttaaagattg aaatttttgc catttaaaaa gaaaagtgac
      361 agataggaat aaaaacttat taaatgtagt tatgatctgt tatatttcat ttctaatact
      421 aggtcttcca caagcagtgt ttcctgaaat gttttctgga gactggggta ttgtcagcta
      481 ttctatatta acattgattg ctatggtttt aggtacttca ttttataatg cttttgtaag
      541 taagcatgtt gaggaatatg aagtattaat gtacttaaaa gagaaaaaaa caagtaatta
      601 atatgagaac accacgcata gtttttattt tgagatatcc tagaatgaaa cacattaaga
      661 acgaaagcaa agcaaaagac gccagaactt ctactacctt ctaagttttt tcatatttgt
      721 ttgttatgct atacatagat ctgcataatt agacttgcgc tgcccgttat tgaagtttca
      781 ggcagcgctc tttttggggg atcgataatg gaagaagaaa aaatatttga gaaacgctgg
      841 cagctagcat caagtgaaca aagagcaaga tacaataatt tgatcacctc ctaccccact
      901 gttgactaga catataagga aaagaaatac ttactttggc tgtgccagtt agatatagat
      961 acctttgaaa catttgaagt gatcctagat aaaattaaac agaattgatc agaaacaagc
     1021 gaagccttct ctcggttcgc ttgttttgct atttattagt tggctcaggg caactctaag
     1081 ctcgtttgaa agaaaaccga tggataatcc ccttgtttgt accactttag aatacatgga
     1141 tgtacgtatt gccattgact cctattagtt actgaaaaaa aatttaaaca agttgagtat
     1201 tgcagataaa tagctccagt gaggtgaata actgctccac tcaaggcaat agatagctcc
     1261 atccagggca acaaactgct ccactttcat atttaataga tgaataacct tctgtataat
     1321 agaaaggcat acctttcggt atgacaactt atacaggatg ttattttatt ggttcactat
     1381 cgaaaaattt tggagcttta tttttccggt atttctcaac gaacgattag ttcaactgtg
     1441 ggaagctcca gacatacgat tcgagaagtt acttctcgag cagacaggaa gggggtgact
     1501 gaactaaccg ctgatatgag cgatacttgg ctgcaggaat atctttttcc ggaaaaacaa
     1561 acaacagcac gaggttacct tcctgtagac tgggaatatg ttcataaaga actaatgaaa
     1621 aagaatatga cattgaaact ccttcataga gaatatgggg tacatggaac atagaaacga
     1681 aaatgcacgg tagaaaagcg aatgaatgta gtataaattc tacaaatcat tcgctttctt
     1741 tattttatat atggttggtc gagagagacc attttctttg gcaatttctg caactggaac
     1801 tccttgattt aacatgtcca ccacagtttt aaagactaat cgtttttgcg gatctgttga
     1861 gttctcagaa tattcaattg gtcgcccctt atatcgacca gcttttttgg ccaataatac
     1921 tccttgccgc tgacgttcac ggattttctc acgttcgttt tgtgcaatat aacttagcaa
     1981 tgataaaaac atatcgaaca tagcagtatc taggagttca tttccagtat taaaatctaa
     2041 aaactgtgca tctagtattt ttaatgctac ttttttctgt ttcataaagg taactgtatt
     2101 ttttatgtct tcataatctc tgcctaggcg atccagtgat gcaacaatga cacaatcacc
     2161 ttctcggata taatttagta atttttgaaa ctcaggtcgc tggatatctt taccactggc
     2221 tttttcttgg aataatttat caatgttaag gtcttccatc attgccaatt gtcgatctaa
     2281 attttgatct tgtgaagaca ctctaatata tccaattttt gccataaaaa ctccttttaa
     2341 aaaatgatat aatttacatg taaaatt
//
LOCUS       DANKKG010000224         2328 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000224 DANKKG010000000
VERSION     DANKKG010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.228"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(<1..210)
                     /locus_tag="PW004_002770"
     CDS             complement(<1..210)
                     /locus_tag="PW004_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDL2727690.1"
                     /translation="MDTITPTNFKENIGKVLQEVVHSNSEIEITRDSKNGLNDGVIMI
                     SKRKWELLQEELYLQRTGTWAYVSDL"
     gene            complement(309..623)
                     /locus_tag="PW004_002771"
     CDS             complement(309..623)
                     /locus_tag="PW004_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727691.1"
                     /translation="MAEENKVVPFETLVINKEEFEQLFLDFFIQYDMIYDFSSSLDLF
                     ELEAEIIENSREKNGKIIISVEKFNELFSQTFEIDPDYYQPQEVFREFRNKIIGFSQT
                     TK"
     gene            complement(691..1185)
                     /locus_tag="PW004_002772"
     CDS             complement(691..1185)
                     /locus_tag="PW004_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727692.1"
                     /translation="MFKVYFPFQWLVKGILLVLLGLILFGSYLTIKARHFPEEKPIKI
                     VTKDLKIGETYQADGVELTFKNIEVTEKRNEFAQHDRVLQVTYTLINDTENPIRPQQW
                     SIENKDGEELYEYDMILDNPEYIESGKEETVNYYYELPIERQDYQIVVRRENGYIVGL
                     PFTL"
     gene            complement(1178..1492)
                     /locus_tag="PW004_002773"
     CDS             complement(1178..1492)
                     /locus_tag="PW004_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727693.1"
                     /translation="MNYPYFKVSASEETKEIFNNFYNQNKGIFGSKANMFRVMVSNLP
                     VLASPSNNKFNDPESIKFEQKISELESMISNEVIEKLDDIDQKLSYSLKNKYKTEEKK
                     DV"
     gene            complement(1708..>2328)
                     /locus_tag="PW004_002774"
     CDS             complement(1708..>2328)
                     /locus_tag="PW004_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HDL2727694.1"
                     /translation="RKEKSQYVERTFSARKLGKRYSFESVSEQLEKNLEESHIKNFFN
                     MEREQVMSLISGVNYKTGELNVPFVELDSIAQKQFSDREGDEKFIKYDDFLREVDKHF
                     VNTMSLEDDQEYTIKLHTDVNPQTLRSAKSKTISIKKTDQKELFLPAKGFENCSHTPS
                     AKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIENQQKRQQNFEMEF"
BASE COUNT          692 a          438 c          311 g          887 t
ORIGIN      
        1 taaatcagat acataagccc atgtacccgt tcgttgtaaa taaagttctt cttgtaacaa
       61 ctcccatttt ctcttagaaa tcataatgac gccatcgttc aatccatttt tagaatccct
      121 agtaatttct atttcagaat ttgaatgaac tacttcttgc aacactttac ctatattttc
      181 tttgaagttt gttggagtaa tagtatccaa aatgaatacc tctcttctct atacctctat
      241 tatgaagtgc ccataaaata aagcaagcgt tgaggaataa ttctatcaac acttgcttta
      301 ttaagctttc attttgtagt ttgactaaaa ccaataatct tatttctaaa ctctcgaaaa
      361 acttcttgtg gttgataata gtctggatct atttcaaatg tttgtgagaa taattcattg
      421 aatttttcta ctgaaataat gattttacca ttcttctctc gactgttttc tattatttct
      481 gcttctaatt caaataaatc tagtgacgaa ctaaaatcat aaatcatgtc atattgaata
      541 aaaaaatcaa gaaaaagttg ctcaaactct tccttattta tcactaatgt ttcaaacgga
      601 acaactttat tttcctcagc cattctccca gttccatttt caaaaatcac aaaactgata
      661 ttaccttaaa aactactaac aaaataaaac ttataaagta aatggcaacc caactatgta
      721 accattctct ctacgtacta cgatttggta gtcctgcctt tcaatcggta attcataata
      781 gtaatttact gtttcttctt tgccactttc aatatactct ggattatcca atatcatatc
      841 atactcataa agttcttcac catccttatt ttcaatcgac cattgttgtg gacgtatcgg
      901 attttctgta tcatttataa gtgtataggt cacttgtaat acacgatcat gttgtgcaaa
      961 ttcatttctt ttttcggtca cttcaatatt tttaaatgtt agttcaaccc catctgcctg
     1021 ataagtttca cctattttta aatcttttgt tacaattttt attggctttt cttctggaaa
     1081 atgtctggct ttaattgtta gataacttcc aaaaagaatc aatcctagta gcactaaaag
     1141 aattccttta acgagccact gaaacgggaa gtaaacttta aacatctttt ttctcctccg
     1201 ttttgtattt gttttttaga gaatacgata acttttgatc aatgtcatcc agcttttcaa
     1261 ttacttcatt acttatcatt gattctagtt ctgaaatttt ttgttcaaat ttaatgcttt
     1321 caggatcatt aaatttatta tttgatggac tagctaacac tggtaaattt gaaaccataa
     1381 cacgaaacat atttgccttt gaaccaaaaa ttcctttgtt ttggttgtaa aaattgttga
     1441 atatttcttt agtttcttct gaagccgaga ctttaaaata tggatagttc atttttttct
     1501 tccacctttc tttttttggg agggagtctg tagggagctt gctccctcag agatttaccc
     1561 gccatctgac ttgccagaaa gtagcctagc gtgagcgggg cggattgact ggcaagtaga
     1621 tgggtagctg gcgtatgtcc taaaaactgc ttctttccaa gcaactcttc ataggcaaaa
     1681 aactggaaag tccactaaca agcccattca gaattccatt tcaaaatttt gttgcctttt
     1741 ttgctgattt tcaatcattt gttgtgtgaa ttcataatcc tttttagcac gatacatttt
     1801 tacatcgtca ttcattatgc gactgatttg atagtgaatc tgtttagcag aaggtgtatg
     1861 agaacaattt tcaaaaccct ttgccggtag aaataactct ttctgatcag tctttttaat
     1921 agaaattgtt ttacttttcg cactacgtaa agtttgagga ttcacatccg tatgaagttt
     1981 aatcgtgtat tcttgatcat cttctaaaga cattgtattt acaaaatgct tgtctacctc
     2041 tcgcaagaag tcatcatatt taatgaattt ttcatcgcct tcacggtcgc taaattgctt
     2101 ttgtgcgatg gaatctaatt ccacaaacgg tacatttaac tcaccagttt tgtaatttac
     2161 tccactaatt agcgacataa cttgctctct ttccatatta aaaaaatttt ttatatgtga
     2221 ttcttctaaa ttcttttcaa gttgttcaga aacagattca aatgaatatc ttttccctaa
     2281 ctttcttgca ctaaaagtac gttcgacata ctgacttttt tcttttct
//
LOCUS       DANKKG010000225         2325 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000225 DANKKG010000000
VERSION     DANKKG010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.229"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..792
                     /locus_tag="PW004_002775"
     CDS             <1..792
                     /locus_tag="PW004_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HDL2727695.1"
                     /translation="FYTIKKYRDELFDWAETYDKIKNFHKQNSTQKKIWENAQDKIRL
                     IEKSRRQTSSQEIDQLIHEMKTILKKADPYSDMMKLNELIQEFIEQYNQLLELESQEI
                     LNKLTEEKQATFKLLEGKDFKDQFVPIVDQEFQELKAKIEQANTIDDLVFLSLNVAEQ
                     YLIFNQRFDNEENRRRQEEQARKQIEAANLIRETEKDEIDSAAKAEEIAIVKPTIQQK
                     TVPMRTLNVHVKTIKSAADIDELTAEINQKLKEQLQENTEITIQF"
     gene            856..>2325
                     /gene="pglX"
                     /locus_tag="PW004_002776"
     CDS             856..>2325
                     /gene="pglX"
                     /locus_tag="PW004_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HDL2727696.1"
                     /translation="MDKKAIKNFAVNARRKLIDEVMTKAKRLGIDESKIEDVKKIDSN
                     LQEIEKSGIRIQGKEIAQRNKLIAELKQRASVTSYSEAFQELIEEVAYTWFNRLIAIR
                     FMEVNDYLPDTYRVLSSEISGKIEPDIITDIYDADIFEELSSNEQQQVRTWKTDNSAE
                     AMDKLYQLIFIKICNQLNDYLPELFEEVEDYTELLFTASYIKKDGVIADLLSIPEDDF
                     YVQAGGQVEIIGWLYQYYNSEPHNQVVNIYKGIVKKQDIPAATQLFTTDWVVRYMVDN
                     SLGKYWLDHYPNSELRNKLKYLIPDKYAFEQATSVSNLKPEELKIIDNAMGSGHILVY
                     AFDVLMEIYLTLGYSERDAARAIVEYNLYGLEIDKRAYQLSYFAIMMKGRQYDRLFLK
                     KSLSPNLREFIDSPKVTDEYQERISELNNDKYSERWNEYKELIFDQFENGKELGSIIK
                     LVDKNNQAITKEYLGKVREYISKFNEFTNMDILYGMKEIQ"
BASE COUNT          930 a          299 c          438 g          658 t
ORIGIN      
        1 ttttatacaa tcaaaaaata tcgcgatgaa ttattcgact gggctgaaac atatgataaa
       61 attaaaaatt tccacaagca aaatagtact cagaaaaaaa tctgggagaa tgcacaagat
      121 aaaattagat taatcgaaaa aagtcggcgt caaacttcta gccaagaaat tgatcaattg
      181 attcatgaga tgaaaaccat tttgaaaaag gctgatcctt attcagatat gatgaaacta
      241 aatgagttga ttcaagaatt tattgaacaa tataatcaac tgttagagtt ggaaagtcag
      301 gaaatattaa ataaattaac ggaagaaaaa caagcaacat ttaaattatt agaaggaaaa
      361 gattttaagg atcaatttgt accaatagtt gaccaagaat ttcaagaact gaaagcaaaa
      421 attgaacaag cgaatactat agatgatctt gttttcttat cattaaacgt agcagaacag
      481 tatttgatct ttaatcaacg tttcgataat gaagaaaacc gcagacgtca agaagaacaa
      541 gcacgtaaac aaattgaagc agcaaacttg attcgtgaaa ctgaaaaaga tgaaatagac
      601 tctgcagcaa aagctgaaga aatagcgata gtaaaaccta ctattcaaca gaaaacagta
      661 ccaatgagaa cacttaacgt tcatgttaaa actattaagt cagcagcaga tattgatgag
      721 ttaacagctg aaattaatca aaaactgaag gaacaacttc aagaaaatac agaaatcacg
      781 attcaattct agtgaaaaga caggcataaa aaggtgcctg tcttttcaaa ggttacatat
      841 agaagggaaa gaaaaatgga taagaaagcg ataaagaatt tcgcggtcaa tgcccgtaga
      901 aaattaattg atgaagtaat gactaaagca aaacgtttag gaattgatga atcaaaaatt
      961 gaagatgtga agaaaattga tagcaattta caagaaatcg aaaaatcagg aataagaatt
     1021 caaggaaaag aaatagcgca aagaaacaaa ttgattgcag aactaaaaca acgtgcttct
     1081 gtcacttctt attcagaagc ttttcaagag ttaatcgaag aggttgctta tacgtggttt
     1141 aaccgattga ttgccattcg gtttatggag gtaaatgatt atttacctga cacgtatcgt
     1201 gtattatcta gtgaaataag tgggaaaatt gagccagata ttattacaga tatttatgat
     1261 gccgatatct ttgaagaatt atctagtaat gaacagcaac aagtcagaac ttggaaaaca
     1321 gataattcag cagaagcaat ggacaagctg tatcaattga tttttataaa aatctgtaat
     1381 caattgaatg attacttacc tgagctattt gaagaagtag aagattatac agaacttttg
     1441 tttacagctt cttatattaa aaaagatggt gtaatcgctg atcttttaag tattccagag
     1501 gatgatttct atgttcaagc tggtggacag gtagagatta ttgggtggtt gtatcagtac
     1561 tataatagtg agccacataa tcaagtggtg aatatttata aagggattgt caaaaaacaa
     1621 gatattccgg cagcaactca actatttacc acagattggg tcgttcgtta tatggttgac
     1681 aatagtttag gaaagtattg gttagatcat tatcccaata gtgaattaag aaataagttg
     1741 aaatacttaa ttccagataa atatgctttt gaacaagcaa catctgtaag taatttaaaa
     1801 ccagaagaac taaaaattat cgataacgca atggggtctg ggcatatttt agtttacgcc
     1861 ttcgatgtat tgatggaaat ttatcttacg ttaggatatt ctgagcgtga tgctgcacgt
     1921 gcaatcgttg aatacaattt atatggtctt gaaatcgata aaagagcgta tcaattgtct
     1981 tattttgcaa ttatgatgaa agggcgtcaa tacgatcgtc tttttttgaa aaaaagtcta
     2041 tcgccgaatt taagagagtt tattgattct cctaaagtga cagacgagta tcaagagcgt
     2101 attagtgaat tgaacaatga taagtatagt gaacgctgga atgaatacaa agagttaatt
     2161 tttgatcaat ttgaaaatgg gaaagaactt ggatcaatca ttaagttagt cgataaaaat
     2221 aatcaggcaa taactaaaga atacttagga aaagtaagag agtatatatc aaaattcaac
     2281 gaatttacga atatggatat tctttatggg atgaaggaaa tacaa
//
LOCUS       DANKKG010000226         2317 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000226 DANKKG010000000
VERSION     DANKKG010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.231"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            342..860
                     /locus_tag="PW004_002777"
     CDS             342..860
                     /locus_tag="PW004_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727697.1"
                     /translation="MLYTEKNDNNIFERHRIIGTIVCITVIPLLISIGILFKFIPNAM
                     VNQMVHIGSPLMVIASIFLFYVFKNFSFYSKYINKLARTSLGVYILHENHYFIDGMKS
                     RGLIWDGIFGLGKRFLDRDFWIWFIIAVVVVCIICMAIDVVREKFERQIFKIKSVKNL
                     CKLMNHVINIYP"
     gene            868..1908
                     /locus_tag="PW004_002778"
     CDS             868..1908
                     /locus_tag="PW004_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDL2727698.1"
                     /translation="MRRKEVKVISFGELLLRLAAPGYSKLFQRDSLETTFCGGEANVA
                     VSLAIFGYQSEFITKLPDSDVGYAAANALRYFGVNVDNIIFGEGRMGLYYLEKGAAQR
                     PSKVIYDRKFSAISLADSDEFKWKEILNDANWFHWTGINPALSENLFETSLDACQAAK
                     KRGITISCDLNYRSKLWTSEEAKEKMEQILPLVDICICNEEDAEKVLGIKAEHTNINS
                     GELNKDGYVQVAREIHKKFGCRYVATTLRRSYSASRNGWSAMLYEAEKDKAYFSTEYN
                     IQIVDRVGGGDSFSAGIIYALCENMSGQKAIEFATASSCLKHTIEGDFNRTTIEDVYN
                     LMNSGGNGRVLR"
     gene            2157..>2317
                     /locus_tag="PW004_002779"
     CDS             2157..>2317
                     /locus_tag="PW004_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL2727699.1"
                     /translation="MTYTYLTTDELVIIESYLKINQSVAKTAHCLNRSSQTIHKVYLF
                     FKQRKSALE"
BASE COUNT          808 a          274 c          463 g          772 t
ORIGIN      
        1 agaaacattc actttgaaaa tgttttggga attgtattag aatgtatttt ttatagtgta
       61 ttagtttatg gaattctttg tgcatgtgga atagaaacat tttcattgaa cagctttgta
      121 aaagaaatat tatcatttgc tctaggtaga tattggttta ttacatccta tttagcattg
      181 tatattctaa gtccgtattt aaatatcatg cttaacaatc tggaaaaact tcagttgcaa
      241 aaaataagta tgttattgtt tataggtgta tgctgttatc gtatgttatt taaaacatct
      301 ttgtttgggg attgtgctgt tttaattgca ctatatgtca tatgttatac actgaaaaaa
      361 acgataataa tatttttgag agacacagaa tcataggaac aattgtatgt ataacagtta
      421 taccacttct catatccata ggaatacttt tcaaatttat accaaatgca atggttaacc
      481 agatggttca tattggttct ccattgatgg ttatagcaag tatattttta ttttatgtat
      541 ttaaaaattt ttcattttac agtaagtata taaataaact tgcgagaacc agtttagggg
      601 tatatatttt acatgaaaat cattatttca ttgatggtat gaagtctaga ggcttaatat
      661 gggatgggat atttggatta ggcaaaaggt ttttagatag ggatttttgg atttggttta
      721 taattgcagt tgtagttgtg tgcataattt gcatggctat cgatgttgta agagaaaaat
      781 ttgaaagaca gatttttaaa attaagagtg taaagaacct gtgtaaactt atgaatcatg
      841 ttataaatat atacccataa ttaataaatg aggagaaaag aagtgaaagt tatatcattc
      901 ggagaattat tattgagact tgcagcaccc ggttattcaa agttatttca aagagatagt
      961 ttggagacta cattctgcgg aggggaagct aatgtagcgg tatccttagc aatatttgga
     1021 tatcaatcag aatttataac aaagcttccg gattcagatg ttggatatgc ggcagctaat
     1081 gctttgcgtt attttggagt aaatgttgat aatattatat ttggagaggg acgcatggga
     1141 ctgtattatt tagaaaaagg agcagctcag agaccatcta aagtaattta tgacagaaaa
     1201 ttttctgcaa tttcgttagc tgatagtgat gaatttaagt ggaaggagat cctaaacgat
     1261 gcaaattggt ttcattggac agggataaat ccagcattgt ctgagaatct ctttgagacg
     1321 tccttagatg catgtcaggc tgccaaaaaa agaggtataa caatttcttg tgatttaaat
     1381 tatcgatcca agctttggac atcagaggaa gcaaaagaaa agatggaaca gatactacca
     1441 ttggttgata tttgtatttg taatgaagaa gatgcagaaa aagtgctagg aattaaagct
     1501 gagcatacaa atattaatag tggagaatta aataaagatg gctatgtgca ggtggctagg
     1561 gagattcaca agaagtttgg atgtaggtat gtagctacaa ctctgcgtag aagttattcg
     1621 gcaagtagaa atggttggag tgcaatgtta tacgaagcag aaaaagataa agcgtatttt
     1681 tccacagaat acaacataca aattgttgat cgcgttggtg gtggagacag cttttctgct
     1741 ggtataatat atgcgttatg tgaaaatatg tccggacaaa aggctattga atttgcgact
     1801 gcatctagtt gtttaaaaca tactatagaa ggtgatttta atcgaactac aattgaggat
     1861 gtatataatt taatgaatag cggtggaaat ggtcgcgtgt tgcgttagtc aacatataaa
     1921 gataaatcac agtaattttt atggtgatta tctgtcttag tagtgattag aataacgtga
     1981 aaataaattg ttgagcgttt atactgtttt gaaaaataaa tttaacatat tgaaaaatac
     2041 aaatatatac gtatattata aagcgccgat tgtaaaatta agctagacaa ctaaaacagt
     2101 catttgtgct acactcaaaa tagttccgac caaagaatta taaggagtga gtgcaaataa
     2161 cctataccta tcttacaacg gatgaacttg taataataga gtcttatttg aaaattaacc
     2221 aatctgttgc taaaactgcg cattgcttga atcgttcaag tcaaacgatc cataaagtat
     2281 atctgttctt caagcaaaga aaatcagcct tagaata
//
LOCUS       DANKKG010000227         2271 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000227 DANKKG010000000
VERSION     DANKKG010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.232"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..749
                     /locus_tag="PW004_002780"
     CDS             <1..749
                     /locus_tag="PW004_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDL2727700.1"
                     /translation="DLKYNAITTWNNIKQFFKDTWKNIKDTAYNTWISIKNTMINTWN
                     NIKDSFWNIVTGIVNSAENAWTNLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGL
                     INGVKEKWNALKDTIKGIAGNIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKE
                     QSKLDQTMTNLVKTPSVQPVVNGISHLPITQTKQNISASETKEIHLHLNVYGDLPDSM
                     VRKITTKIKTELTRQMKRDAGAVGGTLYAT"
     gene            739..1479
                     /locus_tag="PW004_002781"
     CDS             739..1479
                     /locus_tag="PW004_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2727701.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYKNVTRKIECYYKAPSIDMVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            1476..>2271
                     /locus_tag="PW004_002782"
     CDS             1476..>2271
                     /locus_tag="PW004_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2727702.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCGIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLVEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRIIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIA"
BASE COUNT          859 a          278 c          428 g          706 t
ORIGIN      
        1 tcgatttaaa atataatgcc attactactt ggaataatat taaacagttt ttcaaagata
       61 cttggaaaaa tattaaagat acggcataca atacgtggat ttctataaaa aataccatga
      121 ttaatacatg gaataacatt aaggactctt tctggaatat tgttactgga attgttaatt
      181 ctgctgaaaa tgcatggaca aatctaaaaa atggtgtttc aaatgctatt aatcgggtaa
      241 aagaaatctt taattcgtta agggaagtca atttatttga aattggcaag aacatcattg
      301 atggacttat aaatggtgta aaagaaaagt ggaatgcttt gaaagatact atcaaaggta
      361 ttgcaggtaa tatcaaaaat tggattaaag gagctttaga tattcattct ccatcaagat
      421 ggatgcgtga tatggttggt aaaaatattg ttcaaggtat tatcgttggt attgataaag
      481 agcaaagtaa gttagatcaa accatgacaa atttagtaaa aacaccgtct gttcaacctg
      541 tagtaaatgg aataagtcac ctaccaatta cacaaacaaa gcaaaatata tctgctagtg
      601 aaacaaaaga aattcattta catttaaatg tttatggaga tttacctgat tcaatggttc
      661 ggaagattac cacaaaaatt aaaacagaat tgacaagaca aatgaaacga gatgctggtg
      721 cagtaggagg aacattatat gcaacttaag agaggacaat tttttattaa tcaacattat
      781 tcttctgaat ttaatgtgta tattcaaaac aggcctgctt ctgtttcagc tagtcgagta
      841 attgaattga gagagcgaga aggaaatgat tctatcatta ttgataaagg ctattacaaa
      901 aatgtgacta gaaagattga gtgttactat aaagcacctt caattgatat ggtgcaggag
      961 tgggaagatc gaatcactga atggttagat atgtcgtctt atagtgattt tattctttac
     1021 tatgatgaac aatatattta tcaagctgta gtaatagaag gacctgaatt taaaggaaca
     1081 agaaaaacag gaaatattgt cccttttgaa tttacagtga gtattcgacc atttaaggaa
     1141 aattacaatg gtagatttac tattcgacaa acagaaactt ttgaaattta taacccagag
     1201 aagtattctt caaaaccgct tattaaattg agtggttctg gagatgcttc tttttatatt
     1261 aataaggata aatatgattt gaaatcgttg gacagagaat tatttataga ttctaaatta
     1321 gaagaggctt atcgaaaatt agatggtaat ttagaacatc aggatcaggt caccttattt
     1381 ttagattttc catttttata tcctggaaaa aatgaaatta aatggacgaa aaatattcat
     1441 tcatttgaaa taatgcctag gtggtggaga aaagtatgaa accaagaata tatagtccta
     1501 ctgaaacaga tttctcaacg aatggcttag gaattttaaa ggattgtaca agatgtggaa
     1561 tatatgaagt agctaatgga aaatatgaat tagagttgga ttatccttta ggaactagat
     1621 ttgatgaata ttttgaaaat gactatcaaa taaaagcaaa gccaaacgat caagaagagt
     1681 atcatatctt ttttattgat gataaagata tagatacttt tttaaataca gtaactattt
     1741 atgctcagag tcgtacaaat cgacttggaa gacgggtagt cactcttgtg gaagtggact
     1801 ctaaaactgg tcaagaagcg atatcaatta ttgaaactaa aatggataaa aaatctgaca
     1861 tacgacttta ttctgatatt acggctgttt ctagcacaat ctttgaagcg agaaatgttt
     1921 taaattgtat tgctggtgaa caaggatcat tgcttcaata ttggggcgga gaaattaaac
     1981 gtgaaccatt taagctttct ttgttaaagc gaagaggtcg caataatgtt ggaacgattc
     2041 ggtatggaaa agatatgtct ggtttgaagg tcaaattaga ttggacaggt ataaaaacaa
     2101 gaattattcc atatgctgat cctcaaagtg aagtaggtac gactaatcga atttatggtt
     2161 cgccagtaga tagtgcctat attaataact atcctgatgt ctatacagaa catgttcagt
     2221 ttacagaaga acaaggagta aaagacatta agagcttaaa taaaatagct a
//
LOCUS       DANKKG010000228         2263 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000228 DANKKG010000000
VERSION     DANKKG010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.233"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..607
                     /locus_tag="PW004_002783"
     CDS             <1..607
                     /locus_tag="PW004_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDL2727703.1"
                     /translation="DGFKACTDPRQAAYAFERNYERPAATHPERQDYAVNWYNKFKDL
                     KPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNT
                     SAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIINWARGGQVGSWFADGTYGHTGVIR
                     GLANGRMQTYEQNTELGMICGKLDRQYYSASAISSIVIPPK"
     gene            620..1528
                     /locus_tag="PW004_002784"
     CDS             620..1528
                     /locus_tag="PW004_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HDL2727704.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKINYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FERWRQEQEHSFQDWREGQESDYLAWFESIKDILKTIDPGGVMLAELTDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVAFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            1528..2148
                     /locus_tag="PW004_002785"
     CDS             1528..2148
                     /locus_tag="PW004_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727705.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
BASE COUNT          779 a          332 c          532 g          620 t
ORIGIN      
        1 tgatggattt aaggcctgta ctgatccaag acaagcagca tatgcttttg aacgaaacta
       61 tgaacgtccg gcagcgacac atcctgaacg tcaagattat gcagttaact ggtataacaa
      121 atttaaagat ttaaaaccag gaggagctac tggagaagca ggactaaaac atttagagtc
      181 tttgattgga caacgaattg gcaatggtca gtgttatggc ttgtctgcag aatattcagg
      241 atatctgggt ggttgtggca tgggtgctgg aacaaaatat ggtttaactc atgtgatagg
      301 aaatacttct gcagcatctg atattggtat tgcctatgat tggtctgctg ttggttggaa
      361 agtgattcaa aaccctagat atgatcaatt agtagttggt gcaattatta attgggcaag
      421 aggtggacaa gtgggttcat ggtttgcaga tggaacttat ggacatactg gcgttatccg
      481 aggcttagct aatggtcgta tgcaaactta tgaacaaaac acagagttag gtatgatttg
      541 tggaaagtta gatcgccagt attatagtgc aagtgcaatt tcttccattg tcataccacc
      601 aaaataggga ggtgatttaa tggcaaagtg gaatgtcata ttaagtacaa cagaacccta
      661 taattacgtg gggatgattc aagttcgaca aggcaataag aatacagagg ttatggaagc
      721 gactatagtt gaaaatggtc ttccctacga tttatcagaa tgtaaggtat attttgaatc
      781 agttgtaggt gggaaatatc cagtccaatt agaaacaaaa attgtggatg ctaaaaaagg
      841 gaaaattaac tatatttttg ataaatattc catgcagtgt ttacatcgac aaacagccaa
      901 tttcattata tttaaaggag aagacttgat tggaacaact caagacttct cttattttgt
      961 cattaatgct gtttcaaaaa cagaaggaga aatgggttct tattggcaat caatcgaaga
     1021 tttaattgcg gatatgacgg actttattaa tgaaaataaa ggcgatttta cggactggat
     1081 gaatgaaagg aaagaagaat ttgaacgttg gagacaagaa caggaacaca gtttccaaga
     1141 ttggagagaa ggacaagaat cagactattt ggcatggttt gaatcaatca aggatatttt
     1201 aaagacgatt gatccaggtg gagtcatgtt agccgaacta acggatgcgc gagtagatat
     1261 tcaaggagtt cgtcatgcgt ctatttcaga gcgtttgctg gcagatatgg actatttata
     1321 tcagaaattg cgagcaacgc ttttcacgat tgaatacggg gagattgaag tgactgatat
     1381 tttgcaggat gatctctttt cagataatca tgaagtcgaa aaagttggaa ctgtagcatt
     1441 cccgattgaa gaaggagcct tgatcatcgc aaccgttgat gatccaaagc aaaatgtgtt
     1501 cactcttgag aaagttgggg tgatctaatg gctaaaacta aacgaatgat ggaaacggat
     1561 gaaaaaacgg gtgtacaacg ccaattcttt ccaatcacac atgcttccgc agttcttggg
     1621 ttagaagaaa taatagcagg agaagcaaca gttttatctg ttaatggaaa aattggagcc
     1681 gtcgtcatta ctaaagaaga tttgggatta gagaatgttc tcacagaatt accctatgca
     1741 agtgaagaag atgacggcat tatcacggca gaaatgtatc aaaaaatttt aaacagtgga
     1801 gaaggtgact acgtgttgcc agttgccact atcgaccgtt tgggtggcat aaaagttggt
     1861 gaattattga cgattgatga gacagggaaa gtctctgcag tcagacaatc tgatgtcaat
     1921 ttttcgctgg agttaaaaga aaaactcgat tcactgaaaa attatactgc tggagaaaac
     1981 atcactattg atgaagatgg aacaattagt tctacaggtg gtagtgggac tggtggtgtc
     2041 aatcagtcat atgtagatca aaaattccaa gaagctgtaa atcaagcaga aaattacaca
     2101 aacgaaagaa ttccaaattt tacttttgaa aaaattgggg aggtatagag aatgacagat
     2161 atcgtaaaaa taaaacaaag caatgtacag gtttatccac aaactcattg gaatgctata
     2221 gaaggcaaac caacaacggt gaagggagat aaaggcgatc ctg
//
LOCUS       DANKKG010000229         2242 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000229 DANKKG010000000
VERSION     DANKKG010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.234"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            21..1787
                     /locus_tag="PW004_002786"
     CDS             21..1787
                     /locus_tag="PW004_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HDL2727706.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            1810..>2242
                     /locus_tag="PW004_002787"
     CDS             1810..>2242
                     /locus_tag="PW004_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HDL2727707.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLP"
BASE COUNT          805 a          283 c          453 g          701 t
ORIGIN      
        1 ataagtagag gagaaagaaa atgactataa aagtagattt acgaaaaaaa ccatataatt
       61 tagatgaaga tgctatttct tggattgaag agacgattga ttctatgact ttggaagaaa
      121 aaataggtca attgtttgtt aatatgggat ctagtcgtga taaaaattac ttgagtaata
      181 tggtcaatca gtaccatatt ggagctgtac gatataatcc aggaactgct gatgaggtat
      241 atgatcaaaa ctatatctta caaacagaaa gtaaaattcc tctattaatt gctgctaata
      301 cggaagcagg tggtaacgga gcttgtacag atggtactga agttggtgta gaagtaaaaa
      361 ttggtgcaac aaatgatgcg aaatatgctt atgaattagg gagagtttct gggattgaag
      421 catcggctat tggatgtaac tggagttttg ctcctattgt agatattaat agaaattgga
      481 gaaatcctat catttctagt cgatcttttg gacaaaaccc tgataaagtt ttagaactta
      541 gtttagctta tatgaaaggt attcaggaga gtggaatttt gcctgcagct aaacatttcc
      601 ctggtgacgg tattgacgaa cgagatcagc atctatcttt tagtattaat agtttaggta
      661 cggaagaatg ggataattct tttggaaaag tatactcagg cttaatagaa gctggattac
      721 cttcaattat ggcaggtcat atccatatgc cagcttattc aaaacatttt aatcctgatc
      781 tgaaagatga agatttactt cctgcaactt tatcaaaaga attaatcact gacttactta
      841 gaggaaaact cggatttaat ggattagtag ttactgatgc tagtcatatg ttaggaatga
      901 ctggggcaat gaaacgatca gaattattgc ccacttctat tgcagctgga tgtgatctat
      961 ttctattttt taatgatcca gatgaagact ttaattatat gatggaaggc tatcgtaaag
     1021 gaattatcac tgatgatcgg ttattagatg ccttaaaaag aattttaggg ttaaaagcta
     1081 aattgggatt acacaaaaaa gcaaaagaac aaattttatt acctaagcaa gaagctctgg
     1141 aaaaaattgg tttaattgaa aataaatcaa tttttaaaga ggtggctgac caagcaatta
     1201 cattagttaa agataaacaa gatatttggc ctattgtacc agaaaaatat aaaaggattt
     1261 tactagtaga tgtacatggg gtaaaaggtg ggtttggtga agtagttgct ggaaatagac
     1321 ctcaagccat tgatttattg aaacagattt tgtcagcaaa aggatttgaa gttgaaaaat
     1381 acatttcacc tgaagaagaa ttgaaaggat tatcgcaaga agaaaaaatt aaaaatatag
     1441 ctggaatata tgctgctaag agaccaatta aaaatttaac taaaaaatat gacttgatca
     1501 ttaatatagc taatgttaat ccaggcacag ttcaacgaat tgtctggcca gcaagtaaag
     1561 gaactccgga tataccgttt tacattcatg aaattccaac aatttttgta tctgtacagt
     1621 gtccgtttca tttggctgat gtacctcaag taaagactta tattaatact tatgatggaa
     1681 aacaagaaac tttagaaata ttggtggaca aactaatggg tctttctgaa tttaagggag
     1741 taagtccaat agatgcttat tgtggtttaa aggatacatt tatttaatat ttagatagga
     1801 gtggtttata tgttgtatcc cattatgaca gagacgaggc aagtaattga cttaagcggt
     1861 ctctggagat ttaaattaga tacaaatgat gacttacagg aagaacttgc tttagaacca
     1921 ataaatgaaa aagagagtta tccgatttca gttccctctt cttataatga tttatttgaa
     1981 acagagaaca ttagaaatca tgttggatgg gtgtggtatg aaaaagaagt gactcttcct
     2041 aaaagattat tagaagaaag attagttctt cgttttggat ctgcaacaca tgaagcaaaa
     2101 gtttttttga atggaaagtt tattactcaa cacaaaggtg ggtttacacc atttgagtct
     2161 gaaattaatc ggtttgtaaa agacgggaat aatcgaatta cagttgctgt aaacaatatt
     2221 attgacgata gtactttacc tg
//
LOCUS       DANKKG010000230         2240 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000230 DANKKG010000000
VERSION     DANKKG010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.235"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..>2240
                     /gene="esp"
                     /locus_tag="PW004_002788"
     CDS             <1..>2240
                     /gene="esp"
                     /locus_tag="PW004_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HDL2727708.1"
                     /translation="LEKTKKRVLKYSIKKLSVGVASVLVGVGLVLGTTELVQAQDEIS
                     PSTPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTGDTLKGNTIGF
                     EVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFVRINDSLGRPT
                     NIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDNLKTDRLMYRI
                     YLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVSAKYEEPNTQT
                     KDGSGSTGANGAIILDHKLTKNYNFSYSASAKGTPWYANYKIDERLVPYVAGIQMHMV
                     QADKVTYDVSFESGKKVADLAIERRKDHENYGVGSITDNDLTKLIDFANASPRPVVIR
                     YVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQNTYGTGYYYLQ
                     DIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGTGHKAQLVDKE
                     GTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSPGFDKPEEGST
                     VIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSNVTDSTKGIVT
                     VKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDKPDVPLAKDAF
                     SILDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQDGSVGTTDLAV
                     TVSKDI"
BASE COUNT          806 a          348 c          458 g          628 t
ORIGIN      
        1 tctagaaaaa acaaagaaac gagtattgaa gtacagtatc aaaaaattaa gtgttggggt
       61 tgcatctgtt ttggttggtg ttggtcttgt cttgggaaca actgaactag ttcaagcaca
      121 agatgaaatt tctccaagca ccccacttga aacagcgata agctccgtac aagtaggtga
      181 caaagtagct tcggggaata cttttcagga gaatcctggt tatacgaaaa attataactt
      241 ttcggattta caatttagtc ctcaagaact aactggagac acactaaaag gcaatacaat
      301 tggatttgaa gtttacggaa aacataatat tgcagcatca actaaaaact gggaaatccg
      361 tcttcaatta gatgaacgat tagctaaata tgtagaaaaa attcaggtcg atccaaagaa
      421 aggaattgga tcttctagac ggacctttgt aagaattaat gattcgctgg gtagacctac
      481 aaatatttgg aaggtcaatt atattcgtgc aagtgatgga ttatttgctg gggctgaaac
      541 aactgataca caaactgctc ctaatggggt aattacgttt gaaaagagct tagatgaaat
      601 ttttaaagag ataggcatag acaatctcaa gaccgatcgt ttaatgtatc gaatctactt
      661 ggtaagtcat caagacgatg ataaaattgt accaggtatt gatagtacgg gctacttttt
      721 aacagattca gatgatttct ataatagctt agatgtatct gaaaataatc ctgaccagtt
      781 taaacatgga tcagtaagtg caaaatatga ggaacctaat actcaaacaa aagatggttc
      841 aggatccaca ggtgctaatg gagctataat cttagatcat aagttaacga aaaattataa
      901 tttctcatat tcagcttccg caaagggcac gccttggtat gctaattaca aaattgatga
      961 acgattagtt ccatatgtag cgggtataca gatgcacatg gttcaggctg acaaagtaac
     1021 atatgatgtt tcttttgaat cagggaaaaa agtggctgat ttagcaattg aacggcgcaa
     1081 agatcatgaa aattatggtg taggttcaat tactgataat gatcttacca aacttattga
     1141 ttttgctaat gcaagtccac gtccagtcgt tattcgatat gttttacaat tgactaagcc
     1201 attagatgaa atcttagaag atatgaaagc aacagcacaa gttgaagaaa ataaaccatt
     1261 tggtgaagat ttcatctttg attcttggtt gtcggatacg aataaaaaat taatccagaa
     1321 cacttatgga acaggttatt attatttgca agatattgat ggtgatggaa accctgacga
     1381 taaagaagag agcggagaca cgaatccata tatcgggaaa cctgaattag aagaagtata
     1441 tgatgttgac acaacagtta aggggaaagt attcatccac gagttagcgg gaacaggtca
     1501 caaagcccaa cttgttgata aagaaggtac tgtattagca gaaaaaacta tcgctccaaa
     1561 tgaaaaagat ggggctccaa tttcagatac tgtagaattt gaatttacgg gtgtagattc
     1621 aagtaaatta atcgcgaaag atgaattaaa aatccaaatc gtttctccag gttttgataa
     1681 accagaagaa ggttcaaccg ttattaagga atcaccaaaa gcggttgata aacaaaccgt
     1741 ggtagttgga tttaaaccag atgctaaaga atcaattcgg aataataaaa acttacctga
     1801 agatgcagag tattcatgga aaacagagcc tgatacttct aacgttactg atagtacgaa
     1861 aggtattgta actgttaaga tcggaaatcg aactttcgac gtggatgtag agtttgctgt
     1921 aaaagcttct caagctatgg aaaatgatgc aacatacgta cctataacaa caactccaga
     1981 aacgacagtt caaagtggaa aacctacatt tgataaacca gatgttcctt tagctaaaga
     2041 tgccttttca attttagatg tctataataa ggacttcggt aatgcaagtg ttgatgcaaa
     2101 tactggtatt gttacattca ctccagctaa aggtgtagga gaatcggagc cgattactgg
     2161 aacaattcct attaaaattg tttaccaaga tggttctgta ggcacgaccg atttagcagt
     2221 aactgtaagt aaagatattt
//
LOCUS       DANKKG010000231         2212 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000231 DANKKG010000000
VERSION     DANKKG010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.236"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            29..469
                     /locus_tag="PW004_002789"
     CDS             29..469
                     /locus_tag="PW004_002789"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDL2727709.1"
                     /translation="MVPRIFGLTPYIIEDKEMSPYYAQGNLIYVRPKNTNEILVGDII
                     TYYENSGKKISTRRVIAVENNHEDFYTKADTKTYIEPGVVKKRNIIGSPIFQIPYIGL
                     VLSKTAFAWIQGLFFTIAFCLTGITAWNTFVELKKKRRRTQKFL"
     gene            597..1280
                     /locus_tag="PW004_002790"
     CDS             597..1280
                     /locus_tag="PW004_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HDL2727710.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1589..1662
                     /locus_tag="PW004_002791"
     tRNA            1589..1662
                     /locus_tag="PW004_002791"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1623..1625,aa:Arg,seq:tct)
     gene            1821..2180
                     /locus_tag="PW004_002792"
     CDS             1821..2180
                     /locus_tag="PW004_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HDL2727711.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
BASE COUNT          735 a          345 c          450 g          682 t
ORIGIN      
        1 atttttaatt cttattcttc tcgtcgtctt ggttccccga atttttggtt tgactcctta
       61 tattattgag gacaaagaaa tgagtccata ttatgcacaa ggaaatctga tatatgtacg
      121 accgaaaaat acaaatgaga ttttagtcgg agatattatt acatattatg aaaattcagg
      181 aaaaaaaatc tcgacgcgtc gcgtaatagc cgttgaaaac aaccacgaag atttttatac
      241 aaaagctgat acaaagactt atattgagcc tggagtagta aaaaagagaa atattatcgg
      301 tagtcccatc tttcagattc catatattgg actagtcctt tcaaaaacgg catttgcatg
      361 gattcagggg ttattcttta cgattgcttt ttgcctaaca ggaatcacag catggaatac
      421 ctttgtagag ttgaaaaaga agcggagacg aacacagaaa tttttgtaag aatgttaaaa
      481 acataaatgt attccagaga tccaacgact cttctgaaca tgaaacacga gaaaacactc
      541 atgcagcttg tagatttagt cagttgttcg tataatgtgt gaagaggagg agtagtatgc
      601 gcaatgaaat gatgcatcgt cctgtagtca aagaagaact attggatttt atgcgtaccc
      661 agcaaaaaaa attaccgggt gagctaggca aactggaaga agaagctcat gtagcagaag
      721 taccagttat tccccatgaa acagttgtat ttttgaaatt tttacttgga cagcttcagc
      781 ctgaacgaat cttggagatc ggagcagcta tcggtttttc atcaagtgtc atggccacga
      841 caatcggaga aaacgctcat gttacgacaa ttgatcgttt cgatgtcatg atcgagaaag
      901 ctaaaaaaaa ctatgaacgt ctaggactga cagataaagt gactttgctt gaaggtcaag
      961 cagcagatat cttgccggag ttgtctggtc cgtatgactt tatttttatg gatagtgcaa
     1021 aatcaaaata tattgaattt ttacctgaat gcttacgttt gttaagaaaa ggtggcgttt
     1081 tgatggtaga tgacatcttc caaggaggaa cgatcttgct cccagatgaa gagattcccc
     1141 gtggcaaacg agctatccat cgtaaattaa atgaatttct tcgtgttgtg atggatcatc
     1201 ccgatttgac atcgacaatc ttgccattag gtgatggtgt gatcttaatg acaaaggaat
     1261 cagaaacaat cgagttgtga tacatgatta tggcagcctg tagataatcg attgaatcga
     1321 gggctcaatt cgaacttgat gtaaaagttt ctatatttgt aagtcaatta aaggatacag
     1381 gaaatctaaa taaacaggag tagcatgaag tcaatgtagc tcctgttttc aattgataac
     1441 cttagaacag ctgaaggaga atgtctcgtt ttaaaagatt gatttagtag ttgggccaaa
     1501 ataattcatt atttgctaat taaataacta tttcgtattg acagaagcaa tttaattatc
     1561 tataattatt gatgttccgc tgattcttgt catagtagct cagctggata gagcatccgc
     1621 cttctaagcg gacggtcggg ggttcgaatc cctcctgtga cgctaaaaaa ccgttagaaa
     1681 ctgaggtttc taacggtttt tctttgtttt ttgtgccaat attgtttgtt ttcaaaggca
     1741 aataaaataa aaaaacatcc ttatcgagta caattataag gagcaaaaaa ataaaactca
     1801 tcagaaaaga ggaagaagat atgacgatac tatctagaat tttagtgact ctagttgcaa
     1861 ttgaattttt ttatatcatg tatatcgaaa cggtgagaac tgattctgac acgacaagta
     1921 gggtttttaa aatgagtaaa gaagagttaa gcagaaaatc tgttcaaaca ttgtttaaga
     1981 accaaggagt gtacaatgga ctattaggag tgggactact ttatggagct tatctttctt
     2041 ccgcatctaa agaaatcaca agtatgctat taatatctat tttttttgtt gcattatatg
     2101 gcagtttgac tagcgatcgt tggattttac tcaaacaagg tgggctagct ctgttggcat
     2161 tgctttctat tttcttataa atatagaaaa aataagaatt tatctaacag ga
//
LOCUS       DANKKG010000232         2208 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000232 DANKKG010000000
VERSION     DANKKG010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.237"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(150..2147)
                     /locus_tag="PW004_002793"
     CDS             complement(150..2147)
                     /locus_tag="PW004_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HDL2727712.1"
                     /translation="MIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKA
                     LVTNKANQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHL
                     KEATKRLSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVY
                     IKNSGVTDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADE
                     AEKYLAKGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGS
                     NLMLSMNAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHE
                     QGSKEITENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKS
                     SVFNRSGQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRD
                     SFKEFGLGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYT
                     PIQLAQYASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMA
                     ILREGFHQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGP
                     TDNPEIAISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          567 a          495 c          402 g          744 t
ORIGIN      
        1 atttttgtca caaccttttt tgccgagtaa acggtgttca aaagccagtt tctcggtatg
       61 aagtcaaatt taagcaaaaa tgtgagaagc tattttcaca aaatttttct tcatactcga
      121 aactaagcgg ctttttcacg acctcttttt tattttgcca tgaacatatc ataatacgcg
      181 tccattattt ctttagcaat cgtcaagttt atgtggtctt gttcgtctaa taagttcggc
      241 aagacaacac tgatggcaat ttccggattg tctgttggtc cgtatgccac gatattacta
      301 ttgactgtcg caatatctgg atgaccttcc gcaaccgttt ctgcagtccc tgtttttgcg
      361 gaaaggtcca ttttcgcact ggctaatggt ttggctgttg tgtaaggatc ggtaccatgg
      421 acaacttggt ggaatccttc acggaggatc gccatatttt ctgcagaaat attgaccgta
      481 ttttctactg ttgtgccaat ttctttcttc acttcaccca agtttccatt ttcgtcattg
      541 ccatagatcg ctttgactag gtgcggctgt actcgtttcc cgccattagc gactgttgaa
      601 gcatattgag ctaattggat tggtgtatat gtgtcaaact gtccaaatgc caaatcggta
      661 aagttacctg gagcatacca ttctttgcca ttatccgcat tgaacttctt catataatca
      721 acagaacgtg agatcccagg agattcattc ggcaaatcga tcccagtcgt tgtaccaaga
      781 ccaaactctt tgaaagagtc tctcaattgc tgataagctt tagcttcttc atctaaacta
      841 ggtagtccca ttccaggtgt atagtctagt cctaacatct ttaacgccac tttgatcata
      901 tacgtattag aagacaattc cgatgctttg actgcatcaa gagcaacttg tccgcttcga
      961 ttaaagacag agcttttttc tgaagcacct tgcagacgaa tcggctcatc aatcaaaact
     1021 tgatttccgc tgatcgcgtt acttgcccag cctgctgtca aagtaccagc cttgactaca
     1081 gaccctggat caaaggcgct agtaatcgtg cctaaagcat tctcggtgat ttctttggaa
     1141 ccttgttcat gactgaaacc agtcattgct agcacttctc ctgtctgagg attcatagcc
     1201 actgcataag caccaggcga atactgtgct ttgccgttgt tgatcaatgt ttgataattc
     1261 cttttcaaaa tttcttctac ttttgattgg aattctgcat tcatagataa cattaaatta
     1321 gaaccttttt ccccagagaa gatttctttt tgattcgaaa catttccttc attatcaagg
     1381 gaaacttcat attgggtttt ggttccttgc aggacatctt catattgttt ttccaaataa
     1441 ctttgtccta cacggtcatt ccgagaataa ccttttgcta ggtatttctc tgcttcatct
     1501 gctggaagtc cttgcttttc agtcgtcaca ctgccaagaa tacttttcag tgaacttgct
     1561 gcattatatt ctcgtgtcca gtctgtgcca gtcgaaactc ctggaagttc agaagcgcgt
     1621 tctgccacaa cagctaactc ttgatcagta acaccagagt ttttgatata gacagtgttt
     1681 aattcatatg cactattcat ccgtttaaag atggtagcaa tttttaattg ctcctcgttt
     1741 aaattaatct catcagctgt gactttattg accatcgcac tatattgttc gctgttccct
     1801 aattgctttt cttttgccga aagtcttttt gtggcttctt tcaaatgttt gggatcagcc
     1861 agccaaaaat cttttttatc tctatctgtc agattttcat ctaccggaac atcgatcagc
     1921 gagttcagct tcgttgcgat ttcaagcaaa tctttagccg taacttgtgt tcctctcgta
     1981 tatgtgatgg cctgattcgc tttattcgta actaaagctt ttcctgtgga gtcgtagatc
     2041 attccgcgag gagtactttc ttggaccgta atcgtagaag tagacttcac tcgcgcctcc
     2101 atctcttcgc cgttcacgat ctgcaaataa cccaatctag caatcaacgc aacaaataac
     2161 gtaaatataa tgaaaaaaag aaaatttaag cgaaaaggaa catgggac
//
LOCUS       DANKKG010000233         2203 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000233 DANKKG010000000
VERSION     DANKKG010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.238"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            182..406
                     /locus_tag="PW004_002794"
     CDS             182..406
                     /locus_tag="PW004_002794"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727713.1"
                     /translation="MNGSIKKILRSIDKNLMITEVSYETLQKKKTLIVAAVLFPAPCV
                     CRNCGSAVVDGNRKLMVVKNKKKETIVHFE"
     gene            410..595
                     /locus_tag="PW004_002795"
                     /pseudo
     CDS             410..595
                     /locus_tag="PW004_002795"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            789..959
                     /locus_tag="PW004_002796"
     CDS             789..959
                     /locus_tag="PW004_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2727714.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1383..2123)
                     /locus_tag="PW004_002797"
     CDS             complement(1383..2123)
                     /locus_tag="PW004_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL2727715.1"
                     /translation="MTKANRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGL
                     TTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTP
                     GGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVET
                     QAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGE
                     TIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT          618 a          460 c          376 g          749 t
ORIGIN      
        1 ttctttttac ttttattttt tacagtcatc tatgacaggt tgtaaatact atggaacatc
       61 atatggcgtt gtcattttct tgtcaactat ttttcctatt catgttttta taaaaaaaca
      121 tatcgttttc ttgtatgatt aaatcaccac aaaataaaca actgagagaa cgatatgctt
      181 tatgaatggt tctatcaaaa aaattctgag atcaatagac aaaaatttga tgattacaga
      241 ggtctcttac gaaaccttac agaagaaaaa aacattgatc gttgctgcag ttctcttccc
      301 tgctccatgt gtctgtagaa attgtggttc tgctgtggta gatggaaata ggaaattaat
      361 ggtggtgaaa aacaagaaga aggaaaccat tgtccacttt gaataatata atcatatgcc
      421 aatggttata cgcctaaaaa agcagcgcta tacctgtaaa aactgtcgaa gccattggaa
      481 cgctcaaagt tattttatcc gtccaaggca ctcgatttca aatcatgtca gacataaaat
      541 cacttcttta ctgactgaaa aagtgtcgtt attttttatt tcgaaaagtt gttaggtatc
      601 tttgacaacc gttattcgca cattgaaaga gttgagaagc tatttaccaa agtcttccaa
      661 aagaattctg tcttgagtgt tgagggttgt gaatttcgtt ctcacgtttc cgttgaggat
      721 aaactgagtt ttatttccgc ggatggcgag acagaaaaat taatcgagaa aatttaccta
      781 cacaaaaaat tcctcgatta acaaactatt tccttagctg cactaatcct aaagaaatag
      841 aattcttagt tacagacatg aacgccacct actttcagct caccaaacat gttctaccaa
      901 atgcgaaatt ggtgattgat cgattacttg gtttttccac gcctttcaag gaggcgtaac
      961 ctttattcac gagttggttg aagctttccg agacaaaaga cccgacttgt ttttctcctt
     1021 attgacaaag agccattaaa actagagaaa aaattacaac tgatcgctga ttatttgtag
     1081 aaagaaaatc aaagtattac ccaataaact tgagtcatta ggcaaaaaac actgcgaaac
     1141 tcaagaatga ttttcgcagt gtttttgtat attttttatt aactagtatt actacaaagg
     1201 tttatttttc acttctcaac gcagcacaaa attgtcttta cttatttact ggttgggttc
     1261 aaccttctct actccgatac taatatttgt ttgtataccc cttctaaaat cggtagtttc
     1321 cattcaattg atgggtacta gttgatttta aaaactatgc ttattcaatt ttttatttta
     1381 gctcatatat ttcgtttctt tctaaatttt atactgatga aaacaagtag cacacccaat
     1441 attgacaata taaactggct aatgatggtt tctccagttt ttggaagatg cttatttcca
     1501 ctgcttgttg gattgtttcc tcttatatct gagctatcag caacttttct attctctttt
     1561 gttaaaaaga tcgccacttc ttgttctttt tcgatcttga aagttactgg tgtcgcatcc
     1621 aattcatacc ccgttggcgc ttgagtttct accagctgat atgctcctgg tgctaagcca
     1681 tctatcgcca gttttccatc tgctcctgtg gttagacctg actgcagaac ggttccattg
     1741 gcatcttgta actcaaaaac agctccttgc aggacttctc cgctttggtc atcgatcttt
     1801 gtcaacacga caccgcctgg tgtaagtcga ttttctttcg tcaattctac caccgctgtc
     1861 tgacttcttt cgatctcaaa ctcgattggt gtcgcatcca attcataccc cgttggcgct
     1921 tgggtttcta ccagctgata cactcctggt gctaagccgt caatcgccag ttttccatct
     1981 gctcctgtgg ttagacctga ctgcagaacg gttccattgg catcttgtaa ctcaaaaaca
     2041 gctccttgca ggacttcccc gctttggtca tcgatctttg tcaacacgac accgcctggt
     2101 gtaagtcggt ttgctttcgt caattctacc accgctgtct gacttctttc gatctcaaac
     2161 tcgatcggtg tcgcatctaa ttcatacccc gttggcgctt gag
//
LOCUS       DANKKG010000234         2184 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000234 DANKKG010000000
VERSION     DANKKG010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.239"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(148..1074)
                     /gene="cysK"
                     /locus_tag="PW004_002798"
     CDS             complement(148..1074)
                     /gene="cysK"
                     /locus_tag="PW004_002798"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HDL2727716.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            complement(1214..2101)
                     /locus_tag="PW004_002799"
     CDS             complement(1214..2101)
                     /locus_tag="PW004_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDL2727717.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          608 a          485 c          364 g          727 t
ORIGIN      
        1 tgtattttaa tgaaaaaaga cgtaaaacag tcgcgttcac tattctacgt ctctattcaa
       61 ataatttttc tcatcataaa tttttgtgtg ttcttttcat ctttaaaagt tttttacata
      121 aacctatctg gagaaaaacc aagtgcctta tatctcttgg taaagggctg tagataaata
      181 acgttcacca ttgtcgggaa ccacagctaa taccttcttg ccttttccta attcttttgc
      241 gacttttagc gcggcagcaa ttgcagcgcc agatgagatg cctactaaaa tcccttcttt
      301 tcgcccaact tctctagctg tttccatcgc ttcgtctcca ctaatagaaa ggactttatc
      361 atacacagaa gtatccagtg ttttaggaac aaaacctgta ccgattcctt ggattttgtg
      421 aggtcccgct tctttccctt ctaaaatagc agattctgct ggttctaccc cgattaattc
      481 gattttcgga tacacacgtt ttaattctcg gccagcgcca gtgatcgttc ctcctgtacc
      541 gatacctgca acaaacgcgt ccaagccatt tactccaaat gcttgatgta tctcaggacc
      601 agtcgtcttc tcatgcacaa gaggatttgc ttcattttca aattgtaatg gtaaaaagta
      661 gccgttttct tttgccaaac gttctgcttc tctgattgat ccagaaatac catccgcacc
      721 aggagtcaaa atcaactctg cgccgtaccc tttcatcaat aaacgtcttt cgatactcat
      781 cgtctcaggc atcacgatga ttactttata tccttttgca actcctacca tcgacaatcc
      841 gattcctgta tttccagaag taggttcaat gatagtatca cctggtttca gtagaccatc
      901 gtgttctgct ttttctatca tacttaaagc aatacgatct tttacacttc ctcctggatt
      961 aaaaaactca agctttacaa aaacatctgc tgaatcttct ggtacgattt tatttaattt
     1021 gacgattggc gttttgccaa tcaattcggt gactgagtta tagatagctg tcatcttcaa
     1081 ttcctccttg tctttgctct tcttacttat cataatacaa tctgttcatt tatggcgagt
     1141 gattcgtctt tgactagcaa aaaaaagaca aagatatacc atatatggta atttctttgc
     1201 ctttttatct aaatcaaacg tttagttctt gaccaactaa taaagaataa ctggacatag
     1261 acatgccatt tttcgccatt agatcatcaa cagacatgcc aaatttttgt gcaatatccc
     1321 atagcgtatc acctgacacg actgtatatg tctgaccatt atttgtattt acatctgttg
     1381 ttgaagatga tgacgttgaa gtcgtcgtag tagtcgttgt tgttgtactt gtggttgttg
     1441 tagccgaacc tgttgatggc gtatcaaaac gggttaaatt ataagtatta atcaatgagt
     1501 tcaatttggc cccataattt ggatcggtag cataacgacc tgtcaagtac gctgttgcat
     1561 cataaaaact tgttgtattg cttttccaca cgccagcata atgataatcg cccgtggaaa
     1621 aactggttga acgaagaaca ttcgcatggt cttggaagga ttcccaataa gaagaatatt
     1681 ttcggaatgg ctcattcatc gttacccatt gtccgtttac atattctttg gttgccatat
     1741 aaacgacctg tccgttatag cttcctttca caccgaatag attataattt ggcgcactag
     1801 ctaatccgga agtgccatat gcactttcca acaacgcttg agcgatcatg actgaagcat
     1861 acaagtcatt acttgcagca acttctgacg cagaccagcc gatttgatcg ataaacgctt
     1921 gaggatcagc gactgtttga gatccttgtt catctgcatg aacatgttgc ggtaccgctg
     1981 taaccatcat cggcccaact aaaagactcg ttccaattaa aggagcaatc gcttttttat
     2041 tgatcaccat ttttctttta ggtctatggt gccgggcact gcgggtcttc agctcttgca
     2101 ttcaaattcc tcctcaaaat aaaaaaactt aataaaaagt tctcttctta aaacaaactt
     2161 aattaattaa attatacttt taat
//
LOCUS       DANKKG010000235         2110 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000235 DANKKG010000000
VERSION     DANKKG010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.240"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..1335
                     /locus_tag="PW004_002800"
     CDS             <1..1335
                     /locus_tag="PW004_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727718.1"
                     /translation="IDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYYAY
                     GLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVV
                     NLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITT
                     YTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQF
                     PWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQS
                     KHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAAD
                     NNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEIT
                     TKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            1322..>2110
                     /locus_tag="PW004_002801"
     CDS             1322..>2110
                     /locus_tag="PW004_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727719.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHA"
BASE COUNT          704 a          319 c          364 g          723 t
ORIGIN      
        1 atagatgctg cttttttatt tagttgttta tatacttttg caacttatcg aacagttgat
       61 atctattatc gatccgcaat tgcagaagca atcgctctta cgattatccc agctgtttta
      121 tattatgctt atggattgat attcaataga gaaaaaaaag tcatcgcttt atctgtcagt
      181 ataagtctac ttgtgtatac tcatgtctta actacactat tgtgtagtgc attgattgct
      241 tgtatgatta tagttcgttt gtttttaagc aagcaaataa aaaacgatat aaaatttgtg
      301 gtcgtgaatt tagtaaaggc tgtttttttg acttgtgcat taactagcta tttttggtat
      361 cctatgttgc agcagatgaa ttatcaagaa atacacagtc ccttcaaaac agatttgcag
      421 ctagaagcac tttctatttc agatagtatc attcaagcgt taaataacga tattacaaca
      481 tacactatgg ggttattagg attggttgcg ttgatttttc ctttattcat actgaaagaa
      541 atggagaaaa aagaaaaaat catctatgtt ggcgtagttt ctacatgggt atgcgtaact
      601 aatttatttc catggttttt gtttcaaaaa acaccgataa gcttgctcca gtttccttgg
      661 cgtatcttag gtatccaaat ctttttttct tcaatgatca taacgcttgt ttttatgaaa
      721 aagactatga ataaaaaata ttcatggtta atcattggcg ctactgtggt gtttctactc
      781 gtcacgtcta tagcagcaaa agaaaattac gcaaaagtta tccaatcaaa acacggtcat
      841 attgttatca atgaagaaac actcccacgt tatacaacat cagatatggg aggattatac
      901 gattacgccc caacagaagc gataaaagaa aggcctcact tagaaaaaca tgaagtaaaa
      961 gtaggagaat cgtgggaaaa agggaagtat aaagctgcag ataataacat tacttacaca
     1021 gtggcaagca acaaaaagca aaaagtgaca gtaccagtat atgcttattt aggtacacag
     1081 gtaaaagtaa acggtaaagt cgtaaatgac tcttataaaa aacatggctt agtgaatgtg
     1141 gatgtaaatg ctggagaaaa taagatagaa attacaacaa aatatactcc aactgctctc
     1201 atggctttat ggatcagtat tctgacttat tgtttagtgg tgtattcaag ttttaaacga
     1261 tttgcaccta ataagttgaa agattctcga tatgtgacta aaaaggagat aaacaagaag
     1321 aatgaaaata aataaaaaag tagtaatatt gagtggatta attatagtta gtagtatata
     1381 ttctttcatc tttttcaaaa atacacagag tatttatacc actggagatc tttcttttca
     1441 cttatctcga atcaaggggt tatcgactat ttttaccagt ccgattaatt atgagacatt
     1501 caattatact ggatatggtg taaattattt ttatcccttt ctgacttttt ttccagctgt
     1561 aatgttatat tggatgacaa aaaatttgat tgtaagctat attatttacg tttggttatt
     1621 gaacttgtgt acaacactcg tagcttatca ttatggcgag cggtttttaa aacaaaaaaa
     1681 agcggcattt ttatttagtt gtctctatat tttctctgct tatcggacag tagacatcta
     1741 ctatcgttcc gcaattgccg aagctatcgc aataacactt gttatccctg tattatttta
     1801 tgcttatcaa atcatctctg gaaaagaaga aaagtatccg tctgtaaagc tagcattgag
     1861 tatgagtttg ctagtgtaca gccatgtact atcaacatta atgagtacag cattaattat
     1921 catttttatt tttattcgtt tggtttctaa aggtttcaag aatgcagatt ttattgctat
     1981 atttaaaaaa ttatttagtg cggcaggaat gacattggtt ctgacgagtg ctttttggta
     2041 tccgatgttt gaacaaatgc tctaccaaaa aatcaacaaa cctagcgtga caaatttata
     2101 tgctcatgca
//
LOCUS       DANKKG010000236         2086 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000236 DANKKG010000000
VERSION     DANKKG010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.241"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            78..755
                     /locus_tag="PW004_002802"
     CDS             78..755
                     /locus_tag="PW004_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDL2727720.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            745..1887
                     /locus_tag="PW004_002803"
     CDS             745..1887
                     /locus_tag="PW004_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDL2727721.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            1900..>2086
                     /locus_tag="PW004_002804"
     CDS             1900..>2086
                     /locus_tag="PW004_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL2727722.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEI"
BASE COUNT          756 a          271 c          434 g          625 t
ORIGIN      
        1 atctacggtg acggcaacag ttctagaaga ataacaaaaa cattgaatca aatattgaat
       61 gatggggaag atattaagtg gattcaaaaa caaatagctt attaaaatat actagtgatc
      121 catacattat tattggggct ggcggacacg caaaagtcat tattgaaacc ttaagattag
      181 aaaataagtt aatatatgga ttttttgatg ataagcaaaa taaaatgcaa gatctgaaat
      241 atcttgggaa aataaacgaa ttgaatagct gggcggatag caaaaaaaaa atctataaat
      301 ttattgtagc tattggtgac tgtgtagtaa gaaaaaagat tataaaaaaa ttgaatatgc
      361 caagtgagca atatggaata gctgttcatc caagtgctat agtggcaaaa gaaatttcaa
      421 ttggttatgg aactgttatt tttgcgaatg ctgtaattaa cccagatgct gtaattggag
      481 agcatgctat aataaacaca ggtagcattg ttgagcatga ttgtagaatt aataactatg
      541 ttcatttatc tccaggtgta tgcttagcgg gtggagtaca tgtaggtgta ggaactcaaa
      601 taggaattgg ttctcaatgt atccagatga agaaaattgg atcttggtgt atgataggcg
      661 cagggagcac tattgttaag gatattccct cacattcctt agcttatggc aatccagcta
      721 aaattaagaa agaaggaatt aattttgaat tctagaattt tattatcaag tcctcatatg
      781 ggaggaacag aagaaaaata tgttgctaag gcttttaaag ataattggat agcgccatta
      841 ggtgaaaatg tggataaatt tgaagaaagt attacaaaat atactggttc taagggcgga
      901 gcttctgcgt taagttcagg tacagctgcc atacatcttg ccttaagact gcttaatgtt
      961 ggaaaaaacg atattgtctt tgctccttca tttactttta tagctacagt aaatcctatt
     1021 ttatatcaag gggctgtacc agtgttcatt gactcagaac ctaatacatg gaatatgagc
     1081 cctgttgcac tagagagcgc attagaagaa gctgaaagaa acaaccaatt acctaaggca
     1141 atcatcgtag tgaatttata tggtcaaatg gctgattatg aacgaatttt agctattgca
     1201 aaccaattta atgttccagt gatagaagat gcagcggaat cgttaggttc tacttataag
     1261 ggaatttcgt caggcttatt tgggacaatt gggatctatt catttaacgg taataagatc
     1321 atcacaacct caggtggagg agctctagtg tctagtgata aaaaatatgt tgagcattca
     1381 agatttttag caacacaagc aagagatcaa gcaccgtatt atcaacatag cgaaataggt
     1441 tataattacc gtctaagcaa tatttctgcc ggtatcggac gtggacaaat ggaagtattg
     1501 aatgatagag tagctaaacg aagagaaatt ttcaatcgat ataaggaagc tttttctgga
     1561 attgatagaa ttgaaatgat gccagaagtg gatggcatgg tgccaaatcg ttggttgtct
     1621 actttaacga tcaatgatta taattctatt gatcaagtag ttaacgtaat aaataaacta
     1681 aatgaaaaaa acattgaaac aagagctttg tggaaaccac tacatttgca accagtattt
     1741 caaggggtaa aaatgtatta ccatgataat aatcttagtg tgtcagaaaa gttatttaat
     1801 agcggcttat gcttaccttc tggaagtaat atgacagata gtgaacaaga atttgttatt
     1861 gatgagttta aaaaaatatt caagtaggtg tgatatttta tgaacatagg aattattggt
     1921 ttaggttcca tggggaaaag aagacttaga ctcattcaag aacactttaa tgatatttta
     1981 ctatgtggtg ttgatactaa tgaggcgaga cgaaaggaag ttgaaaagga atttggaata
     2041 ccaacttata atagtctaac agaggcaaaa aatttgtttg aaataa
//
LOCUS       DANKKG010000237         2038 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000237 DANKKG010000000
VERSION     DANKKG010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.242"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..181
                     /locus_tag="PW004_002805"
     CDS             <1..181
                     /locus_tag="PW004_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2727723.1"
                     /translation="LDSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELS
                     GKKLKKVNKMNLEVE"
     gene            181..1557
                     /locus_tag="PW004_002806"
     CDS             181..1557
                     /locus_tag="PW004_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2727724.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            1687..>2038
                     /locus_tag="PW004_002807"
     CDS             1687..>2038
                     /locus_tag="PW004_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2727725.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIADCRQK"
BASE COUNT          792 a          253 c          373 g          620 t
ORIGIN      
        1 tctagacagt gttactgctg gagaaataat taatatatta aagacattag ctaaagatag
       61 aaataaatgt gtaatagttg taacacatag taaggaagta gcagattctg cggatatcat
      121 tttagaacta agtggtaaaa agttgaaaaa agtaaataaa atgaatttgg aggttgaata
      181 atgataaaaa atgcatttgc ttatgtaact agaaaaagtt taaaatcatt aattatcata
      241 ttagttattt tatctatgtc aactttaagt ctcattagtt tatctattaa agatgctacg
      301 gacagagctt caaaagaaac atttgctaat ataacaaata gtttttctat ggagataaat
      361 agacaagtaa atccaggaac acctagaggt gggggaaatg taaaaggtga agatattaaa
      421 aagatatctc aaacagatag tatagactcc tatgtaaaaa gaataaacag tgttgcagat
      481 ttagttgatt atgatattat tgaaactcaa gagactcttg cgaatcaatc acctgaaaga
      541 gcaaagaatt ttaaaagaac agttatgtta actggggtta acgactcaac aaaagaaaca
      601 aaatttgtat cagaagcata taaattagta gaaggaaagc atttagaaaa tgaagataaa
      661 aataaaatct taatgcataa agatttagct gaaaaaaata accttaaagt tggagataag
      721 ataaaaataa aatctaattt atttgatgct gataatgaga aaggtgcaga tgaaacagta
      781 gaagtagaaa ttaaaggtct attcgatggg cataatagcg gtggagtaag tgcagcacaa
      841 gaactatacg aaaatacatt aatcactgat attcatacag ctgctaaagt ttatggtaat
      901 accgaagata cagctgtata ccaagatgca acattctttg taaaaggtga taagaatctt
      961 gatagtgtaa taaaagatct tgggaaatta gatataaatt ggagagaata taatttgatt
     1021 aaaagttcat caaattaccc tgcattacaa caatctatat caggtattta ctcaatttca
     1081 aataaattat tttttggctc attaatattt gcaggagttg tagtttcatt attattattc
     1141 ttatggatga atgctagaaa gaaagaaata gcagtattac tatcattagg tatatcaaaa
     1201 ttagaaattt ttggtcaatt cctaattgag atggtattta tatcaatccc cgcattcgtt
     1261 ggttcctatt tcttagctca atatacagct gacaagcttg ggaataatat attgaataag
     1321 gttactggtg atatagctaa acaaatagct aaacaatctg catctagcca attaggtggt
     1381 ggggcagaag ctgaaggatt taacaagaca ttatcaagtt tagatatcaa tgtattacct
     1441 aaattcatga tttatgtagt tatatttatg agtttagtac ttcttgtatc attgattatt
     1501 tcttcattca atatattaag aaggaatcca aaagaattat taattgacaa taattaaaat
     1561 tttcggtgct ttttagcacc aaaaatgaga gtgtaatata aaccgtgtaa gtaaccatca
     1621 atcctaggcg ggtggttgct tcacggtttt tatttttcta aaataagatc aagaggtgat
     1681 tacacgatgg ctagaaagaa aagaaatcct gatgctgaga agttagctga atccattttg
     1741 aatacttatc aacccgaatc tgtagaggat atgcaagatg ttttaaaaga tgtgtttggt
     1801 cccttatttg aaaaaatgct tcaaggagaa ttgaataacc atttagggta tgacgcccat
     1861 tctaaagagc caaagaaaca tgacaatcga cgaaacggtt atggcaataa gacgcttaaa
     1921 acgagctttg gtaaagtaaa cattgatgtt cccagagacc gggaggcttc ttttgaacct
     1981 gagttgattc ctaatagaaa acgagatgtc tccgacatcg cagactgtag acaaaagc
//
LOCUS       DANKKG010000238         2023 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000238 DANKKG010000000
VERSION     DANKKG010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.243"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            133..633
                     /locus_tag="PW004_002808"
     CDS             133..633
                     /locus_tag="PW004_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDL2727726.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            730..>2023
                     /locus_tag="PW004_002809"
     CDS             730..>2023
                     /locus_tag="PW004_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HDL2727727.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQT"
BASE COUNT          665 a          275 c          452 g          631 t
ORIGIN      
        1 gagttttcca aactgattgg cttcttggtg attgctttga ttgcggtagg tctggtcttt
       61 aatgcttctg gtgtgaagga tgtcttactc aatttgttta accgaattgt cggtgcctga
      121 gggggaatca caatggaaca gatacgtgtt tatattgcga accttggaaa atacaatgaa
      181 ggtgagttgg tgggggcatg gtttacccca cccgtcgatt ttgatgaagt gaaagaacgg
      241 attggattaa atgacgaata tgaagagtac gcaattcatg attatgaact gccttttgag
      301 attgatgagt atacaccaat tgaggaaatt aatcggttgt gtggtttagc ggaagagctt
      361 aaaggaacgc caattggtga ggtcgcttca gagattcaac atgccttctt caattctttc
      421 gaggaaatgg tagagcatgt ggatgatatc atctattatc ccgattgtaa tgacatgagt
      481 gatgtagcgt attacttgat tgtagaagct ggtgatttag gcgaagtacc agaacacttg
      541 aagacgtata ttgattttga tgcgtgtggt cgagacttgg agattggcgg gaattatttg
      601 gttacgagtc gaggggtttt tgaactaatg taaatcattt gttagaattg accagaaatt
      661 tctatcgtac ttttactttt ctatttgata tactgaaata gaaaatattt gttgataggg
      721 gtgttactaa tggttggcaa taagtggtac aaatgtgatc ttcatattca ttcaactgcg
      781 agtcagtgct ttcaggataa aaaggttact gctaaggaat gggtagaaga atgtttaaac
      841 aaaggactcg attgtgttgc attaactgat cacaatacag gggaaaatat tgatgaatat
      901 aagaaagtgg ctatcgaaaa tggcttagat ttttttcctg gagtggaatt aacgtgtgga
      961 gaaaatggaa ctcatttact agtcctattt gataaaagtg catgtacaaa tgatatctct
     1021 gatttcttaa ccaagatggg tataaaaaga gaggattggg ccagcgctgc agcttgctca
     1081 atgaaatcag ttttagaagt tattgatttt gctgagcacg agggcttgct cgttattcct
     1141 gcacatatag atgagtttaa tggaatttct tcagcaaaat ttggaaatct aggaaagata
     1201 tttgaatcag aaaatatata tgcagtacag acggttcaaa aagaattttt tgaaaatcgc
     1261 tcacaaatga ttccctcatc taagcgtgac actatctatg atagtgtgaa tgccagatat
     1321 gatggtagag ttggaaaaga tactttggaa tcttggtata aatcagttgt tgaggcagaa
     1381 aaaaataata tgacatttct tagcttttct gataacccac actcgaaagg aaattcaaaa
     1441 catggacttt ggggaatagg cacaagatat tcctatataa aaatgaagga cgaacctgac
     1501 ttgggatcac ttcgcgatgc tttaatgcta ggcgaaactc gagttcattc tgatttttct
     1561 aatttctcca ttaatgaaaa tgaagtttta ttggaaaaat taagcttttc tgggaccact
     1621 cttagcacaa aagaagttgt agtcgaattc agcgataatt taacttcaat tattggagga
     1681 agaggaacag gcaaatcttg tattacaaga ttcttggtct atgtccttgg aaaggaagca
     1741 gaacttgatc aattttctga aatacaaagt gattatcaga actttgcaca aattgaacat
     1801 aatggctctg gaatattttt gaaggataca attgtgaaac tgaatatttt ctataagggg
     1861 acaaaatatc aaataataag aacacaggat agacattcta tttatgaatt tacacaagag
     1921 aatggtctgg ttgaaaaaga aacagaacgg ttgagaatga tttccgataa ggtgagtata
     1981 tactctcaaa aacaaattta tgaaatttca aaaaatcaaa cta
//
LOCUS       DANKKG010000239         2004 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000239 DANKKG010000000
VERSION     DANKKG010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.244"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..378
                     /locus_tag="PW004_002810"
     CDS             <1..378
                     /locus_tag="PW004_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDL2727728.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /gene="yfcC"
                     /locus_tag="PW004_002811"
     CDS             426..1826
                     /gene="yfcC"
                     /locus_tag="PW004_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HDL2727729.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1859..>2004
                     /locus_tag="PW004_002812"
     CDS             1859..>2004
                     /locus_tag="PW004_002812"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727730.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAFT"
BASE COUNT          555 a          340 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt ttac
//
LOCUS       DANKKG010000240         1977 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000240 DANKKG010000000
VERSION     DANKKG010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.245"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            240..>1977
                     /locus_tag="PW004_002813"
     CDS             240..>1977
                     /locus_tag="PW004_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDL2727731.1"
                     /translation="MILTIIIIMGFFFLLLLISNRFWYALTATLIINLLLTISTVIKM
                     EMREEPVFPSDLKILTGLSELLSMVSPVLLIVGGLILLLLLITSIIVQRRLQKQYSLK
                     IHWKRRFISIAVLLGCFSGVFFINHKNSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQ
                     FLNNIDIKIMEEPAGYSKEKINGIMKKYNTKADEINQTRSDWLNNQTIVLNLSESFSD
                     PARVPNLTVPTDPIPYIKSIITEGTGGLMLSVGYGGGTANMEWEGLTGLSISNLSASL
                     VTPYTQLVERQKISPNITDLFDEKIAIHPFTAALYRRKQVFDKFGFDRFYYLDSPDKL
                     TYTDKIEQSPRISDDSAYKETLKALQSNTETSQFIQLSTMQNHMPYDDYYSELNYTAE
                     GTAVIPNRRKELQTYMQGLHYTDTAVKKFIQEIDKIDKPITFVFYGDHLPAIYSGNDS
                     KKYGLEQHQTDYFIYNNKYSREQAATLNKEVVAPNNFGAMALEHANIKVTPYYALLTE
                     VSNHLPALTIDPTESLSNRFNGKQVFVTEDSQIIQEKNLTEKQKEILEDYRLIQYDLV
                     AGNQYSATWATQK"
BASE COUNT          676 a          367 c          338 g          596 t
ORIGIN      
        1 tacaaaaaat ccgtttcatt catcgtttca tcgaatactt cgcttttgat tcgattgaac
       61 aattaactca gcgttggaaa aaaagaattc attggctttt tgttcataaa agttattttt
      121 tagttgcagc atttttttac tgctttacct tcgtgcagat tttcgtttta gaaccaaaag
      181 aaggttggaa agaaactatc caggttgcct taaaaatatt tactcagcga caagcaccca
      241 tgatcctgac aatcatcatt attatgggat ttttcttctt actattgttg atttccaatc
      301 gtttttggta cgccttgact gctaccttga tcattaactt actgctcact atttcgacag
      361 taatcaaaat ggaaatgcgg gaagagcctg tctttccttc agatttaaaa atactgaccg
      421 gattgtcaga actattatcg atggttagcc cagtcttatt aatcgtgggc ggattgatcc
      481 ttctcctatt attgattact agtattattg tacaacgtag actacaaaaa cagtattcct
      541 tgaagattca ttggaaacgt cgatttatca gtatcgctgt attattagga tgtttttcgg
      601 gtgtcttttt cattaatcac aaaaattcgc cttctttctt gctgtttaat ttatttaaag
      661 taaacaagac tttctttaac cagcaaggcg ccgtaaaaga taacggacca gtcattcaat
      721 tcctaaacaa tatcgacatt aaaatcatgg aagaacctgc tggctattcg aaagaaaaaa
      781 ttaatggaat aatgaaaaag tacaacacaa aggcagatga aatcaaccaa acaagaagtg
      841 actggttaaa caatcagacg atcgttctca atttaagtga aagcttcagt gatcctgcac
      901 gtgtccctaa tctgacagta cctacagacc ctattcctta catcaaaagc attataacag
      961 aaggaacagg aggattgatg ttatccgtcg gttatggtgg cggaacagct aacatggaat
     1021 gggaaggact gacaggcttg agtatcagta acctgtctgc ttcgttagtt acaccttata
     1081 cccagctagt ggagcgtcag aaaataagtc caaatattac cgatctattc gatgaaaaaa
     1141 tagccatcca tccttttaca gctgctcttt atcgcagaaa acaagtgttt gataaatttg
     1201 gttttgacag gttctattat ttagacagtc cggataaatt aacttatacg gataaaatag
     1261 aacaaagccc acgcatatcg gatgattcag catacaaaga aaccttgaaa gctttacaaa
     1321 gcaatacaga aacgtcacaa ttcatccaat tatccactat gcaaaaccat atgccttatg
     1381 atgattacta tagtgaattg aactacactg ctgaaggaac tgctgtcatt cctaatagaa
     1441 gaaaagaatt acaaacttat atgcaaggcc ttcactatac cgatacagca gtaaaaaaat
     1501 tcatccaaga aatcgacaaa atcgataaac cgattacctt tgttttttat ggtgaccatt
     1561 tacccgcaat ctatagtggg aatgattcga aaaaatatgg attagaacag caccagacag
     1621 attatttcat ttataacaac aagtatagtc gtgaacaagc agctacgtta aataaagaag
     1681 ttgttgctcc taataacttt ggtgctatgg ctcttgagca tgcgaatatc aaagtaactc
     1741 cttactatgc gttgttgaca gaagtatcta atcatctacc tgcattaaca attgatccta
     1801 cagaaagctt gtctaatcgt tttaatggaa aacaggtctt tgttacagag gatagtcaaa
     1861 ttatccaaga aaaaaattta actgagaaac aaaaagaaat tctagaagat tatcgtctga
     1921 tacaatatga tcttgttgca ggtaaccagt acagtgcaac atgggcgaca caaaaaa
//
LOCUS       DANKKG010000241         1955 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000241 DANKKG010000000
VERSION     DANKKG010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.246"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            73..555
                     /locus_tag="PW004_002814"
     CDS             73..555
                     /locus_tag="PW004_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727732.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSTFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            752..1642
                     /locus_tag="PW004_002815"
     CDS             752..1642
                     /locus_tag="PW004_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HDL2727733.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT          635 a          335 c          406 g          579 t
ORIGIN      
        1 ttttccccct aattttttta gatagaaaat tgtatagtat gatgagagaa aaagtagttt
       61 gggggaacgt taatgagaag aaaagcagta atcgatcggc tttatttatt tttttggttt
      121 tttcctatcg ttttgctacc ttttacatgg gtaaatgctg ttgtacaagg tgcggagata
      181 ttaggcctga tccaaggaag cggatttgtg gtttctagga attttccagc tttgattgtt
      241 ttctatttag tgataagtgt aggtctctta ttaccaaaat ctctgatcaa tcactacaat
      301 tggcagctgc tttacttttc ttgcttatta gtatttacat ctacgttttc tttgctgcct
      361 cgcttgatta tcggccctaa tggtttgagc gagagtggat tgaaggattt agcttattcc
      421 tccatgtatt tcttgacgat caaaccatgg ttctatttag taattgcctc atttattctt
      481 gcaacatttt tttacttctg gacagaaatc aggataaaaa gtgaacgatc tgtatctata
      541 aaaaataact attgatggtg gatcatcagt aaaaaaacat atgagctgcg taaaatgcat
      601 gtttctcata tgcttttttt attattatgt aaagaatgcg aaaaacgttt acatgatttt
      661 tacataactt aactatattt ttacagcaac catacgaggg atttacagtc gactgataaa
      721 ctttagttgt aaataagaaa ggagcaataa aatgaaaaaa gtattgttta ctctaggtat
      781 gatgggaatt cttttagccg gatgcggctc aggatcagga aatacagatg gctctgccac
      841 acaaaataat tcaagtgact caaatgaaca agtaaaaatc gttgcggtag gttcgacagc
      901 attacagccg ttagtcgatg cagcacaaga aagctttgta caagaaaatc caaattatca
      961 aatctctgta caaggaggag gaagcggaac tggtttgagc caagtcgaag ctggagcagt
     1021 tactatcgga aactctgatg tatttgcaga agaaagagac ggagttgatg cttcaaaatt
     1081 agtggatcat aaagtagcag tggtcgggat ggcaccaatc gtcaataaag atacagatgt
     1141 caaagatatc accaaacagg aattgattga tatctttaca ggaaaaatca ctaactggaa
     1201 ggaagttggc gggaaagatc aaaaaatcaa cgtcgtgaac cgtgcaaacg gcagcggtac
     1261 ccgcgcgaca ttcgaaaaat ggggactgga tggtgctacc cctgtgcagt cccaagaaca
     1321 agattcatca ggaacagttc ggcagctggt aagtcaaaca ccaggagcaa tcagctattt
     1381 agctttctca tatctcgatg attccacaca agcactaagt atcgacggtg tagaaccaaa
     1441 agaagagaat gtagcagata acagctgggg aatctggtca tatgaacata tgtacacgaa
     1501 cggaaaacct tcccctgaag tccaaaaatt cttagactac atgatgacag aagaaatcca
     1561 agaaggacct gtcaaagaat tgggctattt accaatcaca atgatggaag tagaacgtga
     1621 tcacgaagga aatatcaaat aactagcaga taaacatgcc acggtagtcg ttactattaa
     1681 ggaaaaatca actatgtttc cccacatata catttttcct ttaattgtga aaaatagttc
     1741 ctcatatttt gccgtttttt gagctaatac cgagggtcag ctgattgaac accgtttatt
     1801 cgaaaaaaag agctacgaca tagaactgtc gtaacttttt ttggctcttc gtcaaatcgt
     1861 gttggtgaag acaaatttga ataaaactta gaccgatatg cggcgcgttc tgtgcgccgc
     1921 atattttttt agttttcaaa gatcagacct tgttg
//
LOCUS       DANKKG010000242         1918 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000242 DANKKG010000000
VERSION     DANKKG010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.247"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(367..1158)
                     /gene="msrA"
                     /locus_tag="PW004_002816"
     CDS             complement(367..1158)
                     /gene="msrA"
                     /locus_tag="PW004_002816"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDL2727734.1"
                     /translation="MLDVEQMEIDLYNIILNRNTREWERTQFEEAKEDLAKMSIKQVI
                     EKLEVNLRPLALRDNLTPDAMDFYLRITGESASAKAYDYAKHKITSPLYQERAIFAGG
                     CFWCMVEPFETKPGVISVLSGYTGGETKKPNYDQVSGGYTGHVEAVEIIFDTRQITYE
                     ELLEIYWQISDPTDAFGQFQDRGKQYRSILFVVNEKQRQLAEKSREQLIQSNRFSRPI
                     VTEIRQAKTFWPAENHHQQFYQKQPKRYKKIKHSHQLHLFFQRHR"
     gene            complement(1349..>1918)
                     /locus_tag="PW004_002817"
     CDS             complement(1349..>1918)
                     /locus_tag="PW004_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HDL2727735.1"
                     /translation="PYILGLFYPKLNQMTTVISVFGDVDSPVRRAFLVWSVVSGLFFV
                     LSLPALYHLFIGTSKTLAILAVATVGLYGIGDCIFTGLFSINTNESSWNLSTWIHNIG
                     SGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTVISLLIAGLYGLARIPSISQFAF
                     FKQLGFFQRLSFFFNYLPMICLSFFQLRK"
BASE COUNT          610 a          400 c          312 g          596 t
ORIGIN      
        1 tatagttatt gtttgcgctt ttatacggaa tttagttcat cattaatgtg gaattcgatt
       61 aggtgtctat taattatcaa gatgattctc tacaaaattc catagaaaaa tcaagattct
      121 acaattcgaa ttctctttca catggaaata aaggctagta aaaaaacagg agttgtgaca
      181 aaagacttgt catacctcct gtttccttgc gacttgaagc tgaatgcttt gcgccacaac
      241 cttttcctcg gtgttctcaa agcaactcct cgaaataatt cataactgtc caaaaatatg
      301 aaaagctatt tttgtcagtt gctcattatt ttgtcggagc tgaacgcttt gcgccacaac
      361 cttcctctat ctatgtcgct gaaaaaataa atgaagctga tgcgaatgct tgatcttttt
      421 atatctttta ggttgcttct gataaaattg ttgatgatga ttttcagctg gccaaaaagt
      481 ctttgcttgg cgaatctctg taacgatagg acggctaaat cggttagatt gaatcagttg
      541 ttctcttgat ttttcagcca attgcctttg cttttcatta acaacaaata gaattgaacg
      601 atattgtttc ccccgatctt gaaattgacc aaaggcatct gtagggtctg aaatttgcca
      661 ataaatctcc aacagctctt cataggttat ttgacgtgta tcaaatatga tctcaacagc
      721 ttcaacatgc ccagtatatc caccactgac ttgatcgtaa tttggttttt tggtctctcc
      781 acctgtataa ccagataaca ctgagattac acctggtttt gtttcaaaag gctcaaccat
      841 acaccagaaa cagccaccgg caaaaatcgc ccgttcttgg tataagggtg aagttatctt
      901 atgtttggca taatcataag cctttgcact cgctgactcc cctgtgattc ttaaataaaa
      961 atccatcgca tctggtgtca aattatctcg tagtgctagc ggacgcaagt tgacctctag
     1021 tttttctatc acctgcttta tcgacatttt tgctaggtct tcttttgcct cttcaaattg
     1081 tgttctttcc cattctcttg tatttcgatt taaaataata ttataaaggt ctatttccat
     1141 ctgctccaca tctaacatga agtcaccctc tttcaagttt tcttcttatt cattcaaaaa
     1201 atgactacct gaaatttagc acaacgcata acaagttaag actaacttgc ttttcgctct
     1261 cctcatctct attcttagtc gaacacatcc acacgaatgg ttacccatag agaaattctt
     1321 ttacttaatc agcttcacac tgctttttct actttcgtag ttgaaaaaaa cttaaacaaa
     1381 tcatcggcaa atagttaaag aaaaaactta agcgttgaaa gaagcccaat tgcttaaaga
     1441 aggcaaactg actaatcgac ggaatccgtg ctaatccgta aagacctgca attagtaaac
     1501 taataactgt taaaacaaga taaaaatggc taagcgtacc ttgaccactt tgacgataaa
     1561 gcagaacaag taacaatggg aaaagtagaa agcctgcata gcctagccca gaacctatat
     1621 tatggatcca agtcgataga ttccaagagc tttcattcgt attgatactg aataacccag
     1681 taaaaataca atcacctatc ccatataaac caactgttgc aacagcaagt attgcaagtg
     1741 tttttgatgt tccaataaat aggtgataca acgcaggcaa cgaaagaaca aaaaaaagtc
     1801 cagatacaac tgaccagacc aagaaagcac gtctgactgg actatctacg tcaccaaaca
     1861 cactaattac ggtagtcatt tgattcagtt tgggataaaa aaggccaagg atatacgg
//
LOCUS       DANKKG010000243         1901 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000243 DANKKG010000000
VERSION     DANKKG010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.248"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            428..1387
                     /locus_tag="PW004_002818"
     CDS             428..1387
                     /locus_tag="PW004_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL2727736.1"
                     /translation="MTYTHLTPTELVMIEAYFNQSHSVSKVAHLVQRSRQTIYNVYRF
                     LKSGGSAISYYEQYKKNKRNCGRRPIVLPDGQQEYIQKKVAQGWTPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDPHDLPMKGKRKPNGHKEKRGKQAFRRSIQDRTTDYIQFNHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPAGRQAIDIENRLNQWFQSVPKHLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNK
                     VSETFIQSVASKRNHIPRKSLHYRTPLEVFLSYVGKDDLSSLI"
     gene            complement(1374..1628)
                     /locus_tag="PW004_002819"
     CDS             complement(1374..1628)
                     /locus_tag="PW004_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727737.1"
                     /translation="MCYFDREQDFFYYGGMGGGSIVPKINLVELDVNNYRELYVFNSL
                     DFEIPMKQMKNIDIHILPSESCQIEYSIEYFYKVSFVKLS"
BASE COUNT          648 a          321 c          291 g          641 t
ORIGIN      
        1 aattacaaaa tatgtgatat aggaagttta gttattgttc tcataatgcc atcctgtttt
       61 tagattatct agtaattttt ttgttattta aaattaggag cagtgccaat tgttttaatt
      121 ttaaaggctt tttttatatc ttctaaatta gaagatattt gtatatcaat atggaatata
      181 ttaccttttt tgtatttttt tatgttatct ttagctaaaa catcaaatac atgtccttga
      241 aattgtatcg aggcagctgg atttagttta aattgaggaa actttaccac agctgattca
      301 aaacacttgt cacatttttt tattttttta ccttcgccga ttgtaaaatt aagctagaca
      361 actaaaaaag tcatttgtgc tacactcaaa atagttccgt cgaaagaatt ataaggagtg
      421 agtacaaatg acctataccc atcttacacc aactgaactc gtaatgatag aagcttattt
      481 caatcaatca cactctgttt caaaagtagc ccatctggta caacgttctc gacaaaccat
      541 ttataatgtc tatcgttttc ttaaatctgg tggaagcgca ataagctact atgaacaata
      601 taagaaaaac aaacgtaact gcggaagacg tccgattgtg ttaccagatg ggcaacaaga
      661 atacattcaa aaaaaggtgg ctcaagggtg gactccagat gtccttattg gtcgagcaga
      721 gtttcctatt gcttgttcgg tacgtacact atatcggatg tttaaagata aaacctttga
      781 ccctcacgat ttaccaatga aagggaaaag aaaaccaaac ggacataaag aaaaaagagg
      841 aaaacaagct tttcgccgtt cgattcagga tagaacaact gattatatac agttcaatca
      901 tgagtttggt cacctagaag gtgacaccat tgtcggtgga aaacataaaa gtgcggtgat
      961 cacgttagtt gagcgattat ctaaagtgat catcaccttg aaacccgctg gtaggcaagc
     1021 tatagatatt gagaatagac tgaatcaatg gtttcaatct gtaccgaaac acttgtttaa
     1081 atccatcaca tttgattgtg gaaaagagtt ctcgaattgg aaaaagatta gtaataccaa
     1141 tgatatttcg atttatttcg ctgatccagg aacaccttct caacgaggac taaatgaaca
     1201 ttcgaatggc ttattacgta aagatggctt atataaagct atggatttta ataaggtatc
     1261 cgaaaccttt atccaatcgg tggcatcaaa acgaaatcat attcctagaa aatcattaca
     1321 ctatcgcaca ccattggaag tatttttgag ttacgtaggt aaagatgatt tgtctagctt
     1381 aatttgacaa acgaaacttt gtaaaaatat tcgattgaat attctatttg gcacgactca
     1441 cttggcagta tatgtatgtc aatatttttc atctgtttca taggtatttc aaaatctaaa
     1501 ctattaaaaa catataattc cctgtaatta tttacatcta actctactaa atttatcttt
     1561 ggaacaatag atcctccacc cattccacca taataaaaaa aatcttgttc tctatcaaaa
     1621 tagcacatat taagctcagt ttgtttatca tcctcaataa ttttataaat caaacctata
     1681 taatcatgaa tgctggcaaa aatttttttt aagtcaagtt ctaaatcttc ttcgaaaaca
     1741 catttaattt ctcctactga taattgatac tctatattac atgacttaat tagtttttta
     1801 gatcccccct ccagataggc atatgtttca ataattatgt tctttttatt gcttggcaaa
     1861 tttccgttat ttattgcata aattaaaaat gactgtctat t
//
LOCUS       DANKKG010000244         1868 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000244 DANKKG010000000
VERSION     DANKKG010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.249"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(58..408)
                     /locus_tag="PW004_002820"
     CDS             complement(58..408)
                     /locus_tag="PW004_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727738.1"
                     /translation="MIDYNDRGRLKWTGFYLSDHTEEIDADNEQRANQNLAKEQITTE
                     EISEVLNAAVIKNRLVCIQKEERNAEGYYPYDIVGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(401..1726)
                     /locus_tag="PW004_002821"
                     /pseudo
     CDS             complement(401..1726)
                     /locus_tag="PW004_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
BASE COUNT          507 a          395 c          302 g          664 t
ORIGIN      
        1 aatatttgta tataactata catgttatat actttagctc atattgctgg ataatgttca
       61 ttctagtcga ctccacttat tatcctggta aaattcaatg tgccgtattt cgtcgtagtg
      121 aattttgtct tttccaacgt agattcccag ctcatcacta cctaagatct ttccaacaat
      181 atcgtatgga tagtatcctt cagcatttcg ttcttctttt tgaatacata ctaatctatt
      241 ttttatgact gcagcgttca atacttccga aatctcttca gtcgttattt gttcttttgc
      301 taagttttga ttggctcgct gttcattatc tgcatcgatc tcttccgtgt gatcggataa
      361 atagaatcct gtccatttca atcgtcctcg atcgttatag tcaatcattt ttaagccccc
      421 ctgcaccgcc tgcatggcca ccaaccaacg tgcttctagt aatactacga gcgccttcta
      481 acatcgaaga agcatgtaca attgacgtaa aaccatactt ctgcctaatt ttatctatta
      541 ttttgtctag tttttcgtca tcaatctgtt tttgtggatc actaaacaag tccagctgaa
      601 gcgaatcggt gtacacaagc ttgctatagg ttattccaac gtgccttact tcttgtcctt
      661 cataatactt atcaaacaag aaaagacaat aattctgcaa ttcctttgta ttatctgtga
      721 cagggatctt catctggtgg aaaaatcctg gtctggtctc taagatcgac gtaccaatgt
      781 ttaaatggat acatcctgtt ttacaattat gctgtctgat acgaatggct acttgttccg
      841 ccatttcttt tatcaccagc tcgatttcat ttcttctagt ataatctcgt ggtaacactt
      901 gtgaatttcc atatgatttt tcctttgtcg gttctggtgg gactgctatg tctgttcgat
      961 cgattccatt tgcatggaag tacagctgta agccaatcac acctaaacga ctcttaatag
     1021 tgtgtggatt ccaattagct aagtctttaa tactcatgat acccatttag ttgagccttt
     1081 ttttcattcg agagccaatg ccccaaagat ccgtcatttc aggaatattc catacttttt
     1141 cgggaacgtt ttcgtacgtc cattctgcaa caaatccttc attatgcttg gcttcattgt
     1201 ctaaggctag tttagccaaa aggggattat ctccgatacc taccgtagca atcaatccta
     1261 gctcttcttt aatattctcc tggatcattt gagccagctt ctttctccgt tgacttcgtg
     1321 ttccttcagt cgtaaaaagg ttcagtgatc tggtcacttt taagatcgat tcatcgatcg
     1381 agtaaatcaa taaatcttcg tctgctacat atcttctgaa gatattgttt acctgcatgt
     1441 ttcgcttaat atagagattc attcgtggtg gaacgacatg taacgtttta ggaaatggtt
     1501 gtggtaagtc acgtggtcta ctgacattag aaataccata cttcttcttt gcagctggtg
     1561 aagaagctaa aatcaaccct gatcctgtat tatctgctag actcatgacg actaattcag
     1621 tcgttaatgg atctaaaccc ctttctatac attcgacact tgcgtaaaag cttttcatat
     1681 cgatcaaaaa ggaagtattt actggctctt ttgaataatc tatcataaat aacactccat
     1741 tcgtcacaat tatacaaaca tatgttccct tttgtaaaga aggaagttca aatgaacaaa
     1801 aaaagattcg aattgttatt acaaaaagtt taaacggata actgaccact tattagattt
     1861 taaatgcg
//
LOCUS       DANKKG010000245         1858 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000245 DANKKG010000000
VERSION     DANKKG010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.251"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            488..1858
                     /locus_tag="PW004_002822"
                     /pseudo
     CDS             488..1858
                     /locus_tag="PW004_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179228.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          693 a          311 c          333 g          521 t
ORIGIN      
        1 agtgacaatc tctgaaaact gtccattaaa tgtttcttcc tatcttgttc attaacaaaa
       61 aattataagc ccttacaacg aatattatat attaaattta attgtaggtg aacacgaatt
      121 ttataaatat ttatggtgtt aatgacaaat gatatccgac tcctcttcat acgttctgga
      181 ctatttgcta atagattaaa atttcagcat ctatttctga ccataaataa atgagctgcc
      241 gaaaagctgc caaatcagac ttgaaaataa aggaacaaaa acttttaagt ttctgacccc
      301 gtaactcaca cttcaaaatc ttacttaaaa aggagtctga aagaaaaaaa gagtattcaa
      361 aatttttttc taactagcta catcacatct atgcattgac actcaaagaa ccagaataag
      421 tcatcagaaa gcaagggggt gcggacataa tcctggacga aaaaataaac ttaggaggaa
      481 tgtccgtgtg ttttcccaca aagagcgtgt aagggcaatt cagttatttt taaaatacga
      541 ttgctcttat gctgcaacca ttcgagaatt agggtatccg tcgattggtg cactacggaa
      601 atggtataaa gagtatttgg aatccggaga gctccatcag gagcaccgaa aaaaatcaaa
      661 gtattctgaa gaacaaaaac gaatagcggt gaatcattat tttgaatatg gtcaatgtta
      721 tgcacgaacc attcgcatgc ttggttatcc taatagagag tccttgcggc agtggtgtga
      781 agaacttgca ccaggagcta gaaaactccg aaaaagtgcg gtaaagttaa cacaagatca
      841 aaaagaagct gtgttgaaaa aattctataa accgcaaact aataggaaaa atttagccga
      901 atctgaagga attagtagag ttactctata tcaatggaaa aacacggttc ttggaaagga
      961 ttttccattg cgtatgactt taaaaaatca ggaaaaacaa aaagagctac ttttaaagga
     1021 agtagaagac ttacaaaaac aagtacacca attacagctc gaaaaagcat tactcgaagg
     1081 agcggctgaa ctgctaaaaa aagaaaaagg cgtcaatctt ctttgtctat caaatcaaga
     1141 aaaaacgata ttgattgatg ccttacgaaa tcagttcacc ttgaaagagc ttcttcaaca
     1201 gctccagttg cctaaaagca gctattttta tcaaaaacaa gcacttgaga aaccagataa
     1261 gtattacaaa gaacgtcaat taattatcac gattttcaat cataacttct gtgcgtatgg
     1321 ttaccgtaga attcaccaag ctttaaaaaa tatgggtaaa aagctttctg aaaaagttgt
     1381 acgtagattg atgactgagg aaaatctatt tgttaaattt tctagacgaa aaaagtattc
     1441 ttcgtatgct ggggaaattt ctcctgcaca ccccaattta ttaaatcgga attttaaagc
     1501 aaagaagcca aatgaaaaat ggttaacaga tattactgaa tttagaattc ccgcagggaa
     1561 aatctactta tcaccacttg tcgattgtta cgatggtgct attgttagtt ggactattgg
     1621 aacaaagcca acttgcttca ctctatgtct aaaaagggtt attcaccaga taattcagcg
     1681 tgcgaaggtt tctttggacg attaaaaaac gagatgtttt atcaacgtga ctggaagaat
     1741 acaacaatca atcagtttat aaacaaattg gatgattata ttcattggta taataatcaa
     1801 cgaattaagt tatctttagg agggctaagc cctcttcaat atcggaagaa gcaaagat
//
LOCUS       DANKKG010000246         1834 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000246 DANKKG010000000
VERSION     DANKKG010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.252"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            469..1641
                     /locus_tag="PW004_002823"
     CDS             469..1641
                     /locus_tag="PW004_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HDL2727739.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFRE
                     AVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRL
                     KSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT          642 a          340 c          301 g          551 t
ORIGIN      
        1 aaaaaagttc aaaagatcaa gtgtgcgctt cgctcccact cccctcgacg ttttagtagc
       61 ctttccctca cttcgttcag tccaagccaa ctaaaaagtt ttcgggatac attctccttc
      121 tcttcctaat aatcaattaa aattttaatc tttatatttc tgctaatcac tcaggaaaca
      181 gtacagaaaa acaaacacgt atcatagata taaatgtaat ggcatagtgc gggttttatt
      241 ttcagcctgt atcatagcta aacaaatcga gttgtgtgtc cgtttttggg cgttctgcta
      301 gcttgtttaa agtctcttga atgaatgtat gttctaagtc aaaaaactct gacagcgcct
      361 ttatatagat aaagtccgta taattgtgta aaagtaaaaa ggccatataa cagtcctttt
      421 acggtacaat gtttttaacg acaaaaacat acccaggagg acttttacat gacccaagta
      481 cattttacac tgaaaagcga agagattcaa agcattattg aatattctgt aaaggatgac
      541 gtttctaaaa atattttaac aacggtattt aatcaactaa tggaaaatca acgaacagaa
      601 tatattcaag caaaagaata tgaacgaaca gaaaaccgac aaagtcaacg aaatggctat
      661 tatgagcgca gctttacgac acgtgtaggc acgctagaat taaaagtacc cagaacacgt
      721 gatggccatt tttcacccac agtgtttgaa cgttatcaac gaaacgaaaa agccctcatg
      781 gcttcaatgt tggaaatgta tgtatcaggc gtttcaactc gtaaagtatc aaaaattgtg
      841 gaagaacttt gtggtaaatc cgtctctaag tccttcgttt ctagcttaac agaacagcta
      901 gaacctatgg ttaacgagtg gcagaatcgt ttattatcag aaaaaaatta tccttactta
      961 atgaccgatg tactctatat aaaagtacga gaagaaaatc gagtactctc aaaaagctgt
     1021 catatagcga ttggaataac caaagatggc gaccgtgaaa ttatcggctt catgattcaa
     1081 agtggcgaaa gcgaagagac ctggacaaca ttttttgaat acctaaaaga acgcggttta
     1141 caaggtacgg aactcgttat ttctgatgcg cacaaaggat tagtctctgc cattagaaaa
     1201 tccttcacca acgtaagttg gcaaagatgc caagttcact tcctaagaaa tatctttacc
     1261 accattccta aaaaaaattc aaaatctttc agagaagctg ttaaaggaat ttttaagttc
     1321 acagatatta acttagcgcg tgaggctaaa aatcgattga ttcatgatta tatcgatcaa
     1381 ccaaaatatt caaaagcttg cgcatcattg gatgatggat tcgaagacgc ctttcaatat
     1441 accgtacaag gaaattccca caatcgacta aagagtacca atctaattga acgactgaat
     1501 caagaagtac gcagaagaga aaagattatt cgcatcttcc ccaatcaaac atcagccaat
     1561 cgcttaattg gagccgttct tatggaccta catgatgaat ggatttattc ttcaagaaaa
     1621 tacatcaatt ttgataagta gaaatggtaa aaacattgta tagcatttta cacaggagtc
     1681 tggacttgac tcacttcctt tattattttt cattttttta aactatttaa tactaatgtc
     1741 ttttataata gcttttcata tattatataa tcaatcttta taagtccttt tataaatttc
     1801 ttttctacca ttttcgataa attcctgttt aata
//
LOCUS       DANKKG010000247         1818 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000247 DANKKG010000000
VERSION     DANKKG010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.253"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            275..1753
                     /locus_tag="PW004_002824"
     CDS             275..1753
                     /locus_tag="PW004_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2727740.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
BASE COUNT          661 a          253 c          324 g          580 t
ORIGIN      
        1 taaaagagca ggacggtggc cgtcctgctc ttttttgtgg ttgtttacgc aaatggcgca
       61 aatcttgtac attttaacac aataaaacaa aaaatataga atatttcctc ataaattttt
      121 ccattttttg caatattctc aaaaaaaaga taaaaaatga gaataactga tgaaaaaaca
      181 agcataatcc atcataaaat cagtataggt gaaaagatga actttcatct gcatatcaag
      241 taattggtat gaggcatttt ttgaggtgta ggatatgaag ttggattacc ttatagaaga
      301 ggaagataga gaaaaactgc tgctagctag atatttagaa tttgagaaaa agaattatgt
      361 agtgctgcct aagttatatg aaacattagg tttgtctaaa tttaaaacaa gaaattatat
      421 catagaatta aataaagaat ttactgaatt caaaacaaag ccagagatcc aaatttcaga
      481 agaagcagag atccatgtcg aacgtctgaa tttgacggat atcaaaaatt taagaagaat
      541 ctactttcaa cgatcaaaaa ttgctcgact gttaacggag atcgtggatt caaaagtaac
      601 gatcgaaaaa tttgcagaga atcatttttt aagcatatca agagcctatg taaaaagaag
      661 agaattagtc tcttttcttg aacagcatca aataaagtta aagaaaaaca tattagttgg
      721 ggaagaaata aacatacgga acgtattatt tggggtatat ttcggggtct ttaatggttt
      781 ttctatgcca tttagagaaa gcgtcatgaa tgaagcaaag agtatcgtgc aatattttga
      841 atatctgttc aatttaaaac tgtctgcaac aaaacgaatc aaattcaatt tgctaaccac
      901 tattagtttg ttgagggcct atgggaatca accaatcgga aaacgtttct tttcagatga
      961 atactttaag agctcagaag gaatgaagat catcaaggat ttatcacata tttcttttct
     1021 tcatatccct aaagagcaga tcattgatga gatttcttat atcatgttgt ttttgatcac
     1081 ggaagaagaa tttgaattag atggcgcatt tggacttgcg gaagctcatt ttcatgatat
     1141 ccacatgatt agtcaaaaaa tgtcagtgga attgattgat caattaccat tttcgattga
     1201 aatctctgaa gaagaaagac tatatatacg aaaagaactt cgtatgggta tgatgcatgt
     1261 caatcgaaaa aattggatat tcgactttga gatgactagc tttacgtcta aaaagcagct
     1321 acagtttttc ttcgagacgt atccaatatt cagtacagca atccaaaagg tcatacaaga
     1381 gaataaacgc tatttcttgg gaaaagatga acgtttcatg attcgcttat tttatgatta
     1441 tctcttttta ttggtcggta tttgtccggt atcatatttt gaagcaccga ttcatgtctg
     1501 tattgatttt tcgcaaggat ctgcttatac cgattacatt atttctcaag tgaaaggatt
     1561 taaaaattac aatattgtga ttgagagtcg aatcacttct cgtacgaata tttatttatc
     1621 tgattgttta cttgaaaaat ttaataaaga acagataatt tggaaaaatc caccgactcc
     1681 agatgactgg gaatattttg gaaataccat catcagagaa aaaaatagac atttaactga
     1741 aaaaatcagc tagtatttat ggatggcagc tttgtctcac atatttatta gctgatttat
     1801 gtttttttca aagaaaag
//
LOCUS       DANKKG010000248         1806 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000248 DANKKG010000000
VERSION     DANKKG010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.254"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(1371..>1806)
                     /locus_tag="PW004_002825"
     CDS             complement(1371..>1806)
                     /locus_tag="PW004_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HDL2727741.1"
                     /translation="PEELKDKRVEEVDRTPAENLAYQVGWTNLILKWENDERNGLSVK
                     TPSDQFKWNQLGELYKWFTNTYAHLPLKELEEILNRNIDDINMMIDSMSDEDLFTAHK
                     RKWADEATKTAVWEVYKFIHVNTVAPFGTFRTKIRKWKRLAL"
BASE COUNT          586 a          343 c          259 g          618 t
ORIGIN      
        1 agtggttttc ttttacgttc tattcagatt aacgggtagg cttactgtct tttttggcaa
       61 taattttgca atcaagccat ttacagatgt aatgcgcgga tacttctgct aagattgcaa
      121 aaaacatatc gaaaaggtaa ttcataatac aacctcctcc ccttgccata ataggccggg
      181 gtagcagaca gcataaatat tataccataa ctgaaaattt cattttagta catttcaata
      241 tattgatgaa ccagcacaat aaaacatgcc atgactaaca gaaaaaaact tattaaacca
      301 gatataactt gtaaacatcc aactatatag aaaacgtaaa ttttctatat agttggatta
      361 agataacgac tatttatttt atcatatttt tactataccc tatactttta cttggtatat
      421 ttatgaataa tcgcaaattt acccctatac tactagtttg taagttagat taataaaaat
      481 actaaagtta tttgttcttt tattaaatac acctacaact tataaattga tggtatttag
      541 ataacacgaa agaataaaaa taacagctga tagtaaagtt tgttgacgat tgctactcaa
      601 tcgaaagtga gttagtgcgt atgaacttgc ttctgaaaac ttatgaaatg aaagaccttt
      661 tacatcttgc agaaatcgtt actgggatta aactaagcaa taaaaactaa tgttcccctc
      721 attctttgaa cttctactat ttgatttatt tattctgata aatgacgatt tcaccttcag
      781 gatcttcaaa tcccgtatag aatttattta agatctcttc agtatcagta ataggatagg
      841 cgataggcca catacccttt gatggttaga ataaactgct caaaatctgt atcgtatgtt
      901 ggctttagca tattaacaaa ggctatctcc aaattataaa ccataagcgt tgaatcgttc
      961 ttaacaattt caattcttcc taacccaaag tcagaagata taattaccgg acttgcctta
     1021 cccttcatgt gggttgttga gattccatat ttaaagggaa ttgtcggtac tatcggcggt
     1081 ctgaaaaaca tcccaaaaat aaaaaaatct ttcccctgct agtctcctaa gtccctttgt
     1141 agcggatatg gcaccataat ctcattttat cgaagtgagt catggtttat ctgttggcca
     1201 accaaaattg atacaatcta acgatgcacc ctctacggca ttctagggga gtgcacttgc
     1261 attcacccaa taaaaaccgg cagttagcaa agaacttatg tagtcgtatg ttcttactgt
     1321 taacatacaa gaggtctcct ttccacaatt gcttagcagt aaacgggaat ttacaaagct
     1381 aatctcttcc attttcttat ctttgttcta aaagtcccaa atggagcaac tgtatttaca
     1441 tgaataaatt tatatacttc ccatacagcc gttttggttg cctcgtctgc ccattttctt
     1501 ttgtgtgcag tgaatagatc ttcatcactc attgaatcaa tcatcatgtt aatatcatca
     1561 atattacgat tgagaatttc ctctaattct ttcaatggta gatgtgcata ggtatttgta
     1621 aaccacttat ataactctcc taactgattc catttaaatt gatcagaagg cgttttaaca
     1681 gataatccat ttctttcatc attttcccat tttagaatta aattcgtcca accaacttga
     1741 tatgctaaat tttcagcagg tgttctatcc acttcctcaa ctcttttatc cttcaattct
     1801 tcagga
//
LOCUS       DANKKG010000249         1789 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000249 DANKKG010000000
VERSION     DANKKG010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.255"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..625
                     /locus_tag="PW004_002826"
     CDS             <1..625
                     /locus_tag="PW004_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2727742.1"
                     /translation="YENILKDINFDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKG
                     KIIENKKNLSSPFLVMQEMDYQFFTESVRSELALGNEIVSKDQQEKILKKMELYKMKD
                     QLPFDLSGGEKQRLLVSIASLSKTNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVV
                     IATHDPEFLYKTCNRIIYLKNGGIKKDIKLRVEDSTLIEQIFNDITK"
     gene            643..1212
                     /locus_tag="PW004_002827"
     CDS             643..1212
                     /locus_tag="PW004_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HDL2727743.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            1322..>1789
                     /locus_tag="PW004_002828"
     CDS             1322..>1789
                     /locus_tag="PW004_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2727744.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLK
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINF"
BASE COUNT          600 a          228 c          344 g          617 t
ORIGIN      
        1 ttatgaaaat attctaaaag acataaattt tgatgtcaaa gttggagatg ttattggttt
       61 ggtagggaaa aacggtgtgg ggaaaacaac cttactaaga atactcatga agattattcg
      121 ccctaccaaa ggaaagataa tcgaaaataa aaaaaatttg tcatcgccgt ttttagtaat
      181 gcaagaaatg gattaccaat ttttcacaga atcagtgagg agcgagttag ccctggggaa
      241 tgaaatagtt agtaaagatc agcaagaaaa aattttaaaa aaaatggaac tttataaaat
      301 gaaagatcaa ttaccgtttg atttatccgg tggagaaaaa caaagattat tggtttctat
      361 agcttcattg tcaaaaacta atttatttct atttgatgaa cctacaagtg ggttggatta
      421 tattaatatg gaaaggattg ctgaccttat taaagactta aaagaaaagg gtgcagtagt
      481 tatagccact catgatccgg aatttcttta taaaacatgc aatagaatta tttatttaaa
      541 aaatggtgga ataaaaaaag atataaaact tagagtagaa gattcaactt tgatagaaca
      601 gatttttaat gacataacga aatagaattg aggagaatat atatgaaaac aaaagattat
      661 atttttattg gtttagctac agttatttcc ctagtaattt actttttttc aataatggtt
      721 agtagtattg gtggggcgtt tggtcacagc atatccccag ggatttttgg attattatct
      781 ggaatcatat ttgtttatat tagctataac taccctagaa aaggaatttt tactatttac
      841 acaattgttt tattgttatt ttttacgtta atgggcgggg cgtacttacc ttggtttatc
      901 agttcgatta gtatggcgat cttagctgat attattctat ctgtatttgg atatgatcga
      961 gcaattcctc aagttgcgtc ttgggctcta atgcaattag gcagtgctgc cggacagtgg
     1021 attcctatat ggttctttac agatcgtttt cgtcaagatt ggatagataa aggacaatct
     1081 gctgcaacaa tggatgcaat gatccactat gcggtaggta tatggggaat catttcagtg
     1141 ttagttgtag caagtttatc tatgattggt gtattgattg gaagaaaagt cttaaaaaaa
     1201 tacaaaaaat agaacgtagt ttaccttcta tctgtttgta catctctaat ttacgctatt
     1261 acggaaagca ttaacagttg taaacgaata agagaagaaa ttgaatgcgg gggaggaaaa
     1321 aatgaaagta tacaaaaaat tatttagtta tgtccctaaa atgaaatact acggatggtt
     1381 agccacttta ttttctgatt tttctgtcgt attaacagtc tatggatatt acagtattta
     1441 caaatttctt aagtctctaa ttattgtagg agatgaatat ttagctaaga gttatgcggt
     1501 tcaaactgtt gcttggttaa ctttaggagc tttattttat attttttctg gcttattttc
     1561 tcatatcctt gggtttcgct tagagactaa tttgagaaaa aagggaatta gcggtttaac
     1621 agaagcaagt tttcgttttt ttgatttaaa ttcttcggga tatattcgaa agacaattga
     1681 tgacaacgcg gctaaaacac atatggcaat tgctcacttg attccagata gtgctcaagc
     1741 catagtaaca cctgttttat cagttgcctt aggctttttc attaatttt
//
LOCUS       DANKKG010000250         1755 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000250 DANKKG010000000
VERSION     DANKKG010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.256"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            34..750
                     /locus_tag="PW004_002829"
     CDS             34..750
                     /locus_tag="PW004_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HDL2727745.1"
                     /translation="MVDNYERKNILKKKSIGCRMKIVAIVPMKLNNKRLPQKNTKAFT
                     NGKPLCYYILSTLLKVKQIDKVYVYCSNPDIKEFIPEGVTFLKRSSSLDQDTTSITQV
                     LAGFTKEVEADVYVMTHTTAPFISEKSIEKGLDAVLTGEYDSAFAAKKLQDFLWKDGE
                     PFNYELDNIPRTQDLPPLYEETSGFYIYNRNVMTELHRRIGKKPCIVEVGEIESVDID
                     EAEDFMIADAIYNHLILQGS"
     gene            769..>1755
                     /locus_tag="PW004_002830"
     CDS             769..>1755
                     /locus_tag="PW004_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase catalytic domain-containing protein"
                     /protein_id="HDL2727746.1"
                     /translation="MNNVQILDCTLRDGGYCNNWEFGFDNTKKIISGLIESGIEIIEC
                     GFLTNKTVYNKNVTKYTTLEELAQIIPEKREGKLFVAMMNYGEYNIEDLPDYDGSSID
                     GIRIAFHKKNCNEALKLCREIKKKGYLVFVQAMVSLNYTDEEFLAVIKNVNQFKPYAF
                     YIVDSFGMMKGKDLTRLFYMIEHNLEKDIQIGFHSHNNMQLAYSNAQKLITVQTNRSL
                     IIDSSVYGMGRGAGNLNTELFVEYLNDNAGKKYQLKPLLYIIDEILNSFYQKSYWGYS
                     LPNYISAVHNAHPNYASYLSYKNTLTIEAMNEIFEMMDEEKKVSYDKQYIEDL"
BASE COUNT          685 a          208 c          325 g          537 t
ORIGIN      
        1 gatgttattt ttataaaaag atataattta ttaatggtcg ataattatga aaggaaaaat
       61 attcttaaaa aaaagagtat aggatgtcgt atgaaaatag tcgctatagt accaatgaag
      121 ttaaataaca aaagattgcc acaaaaaaac acgaaagctt ttaccaatgg aaaaccattg
      181 tgttattata tactttcaac acttttaaaa gtaaaacaga ttgataaagt gtatgtctat
      241 tgtagtaacc cagatattaa agaatttata ccagaaggtg taacattttt aaagagatct
      301 agttccttag atcaggatac aacgagtatt acacaggtat tagccggttt cacaaaagaa
      361 gtagaggctg atgtatatgt aatgacacac acaacagcac catttattag cgaaaaatct
      421 attgaaaaag gactagatgc cgtgctgaca ggagaatatg attctgcatt tgctgcaaaa
      481 aaattgcaag atttcttatg gaaagacggt gaaccattta attatgaatt ggacaatatt
      541 cctagaacac aggatttgcc gcctttatac gaagaaacta gtggatttta tatttataat
      601 cgcaatgtga tgaccgagtt gcatagaaga attgggaaaa aaccttgcat agttgaagta
      661 ggtgagattg agtcagttga tattgatgaa gccgaggatt ttatgattgc tgatgcgatt
      721 tataatcatc ttattttaca aggtagttaa gacaggggga aggttaggat gaacaatgta
      781 cagattttag attgcacact tagagatggg ggctattgta acaattggga atttggattt
      841 gataatacaa aaaaaattat ttctggactt atagaatcag gaattgagat tatagaatgt
      901 gggtttctca caaataaaac agtatacaat aaaaatgtta caaaatatac gaccttagaa
      961 gaattagcac agataattcc tgaaaaaaga gaaggaaaat tatttgtagc aatgatgaac
     1021 tatggcgaat ataatataga ggatcttcca gattatgatg gttcatctat tgatggaatt
     1081 aggattgcgt ttcataaaaa aaattgcaat gaagcgttga aattatgtcg tgagataaag
     1141 aaaaaaggct acttagtttt tgttcaagct atggtatctc tcaattatac agatgaagag
     1201 tttttagcag taataaaaaa tgtgaatcag tttaaaccat acgcttttta tattgttgat
     1261 agttttggaa tgatgaaagg aaaagattta actcgtcttt tttacatgat tgagcataat
     1321 ctagaaaaag atatacagat aggttttcac tcacataata acatgcagct tgcatattct
     1381 aatgcacaaa aacttataac cgtacaaaca aatcgaagcc tgattattga ttcttcggtg
     1441 tacggaatgg gaagaggagc aggtaatctt aacacagaac tatttgtaga atatttgaat
     1501 gataatgcag gaaaaaaata tcaattaaaa ccattattat atataattga tgaaatacta
     1561 aatagtttct atcagaagag ttattgggga tattcattac caaactatat ttcagcagtt
     1621 cataatgctc atcctaatta tgcaagctat ctgtcatata agaatacttt aacaattgaa
     1681 gcaatgaatg aaatattcga aatgatggat gaagaaaaaa aggtatctta tgataaacaa
     1741 tatatagaag attta
//
LOCUS       DANKKG010000251         1744 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000251 DANKKG010000000
VERSION     DANKKG010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.257"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            50..562
                     /locus_tag="PW004_002831"
     CDS             50..562
                     /locus_tag="PW004_002831"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2727747.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLY
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            559..1596
                     /locus_tag="PW004_002832"
     CDS             559..1596
                     /locus_tag="PW004_002832"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDL2727748.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT          623 a          331 c          372 g          418 t
ORIGIN      
        1 tcgtatagga taataagtta taataaagac tatacggagg atgattacca tgacaaataa
       61 aaacaatgaa cgttactcta ccgaactgaa agcgacagcc atcaagaaaa tgatgccacc
      121 cgaaaatagg gggattcgcc aattaagtca aactttgggc attccagaag cgactcttta
      181 ttcttggcgt aaaaaagcta gaattcaagg gaatactaca cccggaacga aaaaaaatgt
      241 cgaaaaatgg tcaagtgaag ataaattttt aattgtgatg gaatcattct caatgaacca
      301 agtagaactt gcggaatatt gccgaaagaa gggcttattt aaagaacaaa ttgaggcttg
      361 gaagaaaacg tgcttatctg cgaatgaaca ggaagaaaat cgaacgagag aacttgcaac
      421 tgaactaaaa gaagagaaaa aacaagcgcg tcaattagag aaagaattac gtcataagga
      481 aaaagcatta gcggaagccg ctgctctctt attactaaga aaaaaagcac acgcgatttg
      541 gggggaccaa gaggaagaat gatttcatct tcagatcgca aacaagctgt tgaacttatt
      601 caagaagcca atcgcaatgg ggcccgtctc acctatgcct gtaatgagct gaatattagc
      661 gttcgaacct acgaacgatg gacgcgtgaa ggtacgatag cgcatgatca acgccctttg
      721 gcgaaacgtc ctgtacccaa aaataaactg acggatcaag aacgcgaaaa aataatagaa
      781 accgtttcaa aaaaagaatt tatgaatttg ccacctagcc aaattgtacc aaaacttgcg
      841 gattgctcga tttatattgc atcagagtct agcttctatc gtgtattgcg agaaaaaaat
      901 atgcaacatc accgtggccg aagtcaagtt tcacaaaaac gcataccgcc aagtcacctt
      961 gcgacgaagc caaatgaagt ttggacttgg gatattactt ggctaaaagg tccaataaaa
     1021 ggactctttt accggttata tctcatcatt gatttattta gccgtaaaat ggtaggctgg
     1081 gagatttggg aatcagaaga agctcgtcat gccgaaaaac ttattaaaca gacggtgctt
     1141 aaggaacgta tccaaggaca gccgttggtt ttacactccg ataatggcag ccctatgaaa
     1201 gccgcgactt ttcaagtctt actcgaaaaa ttaggaattc aaagttctta ttcaagacca
     1261 cgagtgagta atgacaatcc ttattcggaa gcggctttcc gaacgttaaa atatcgacca
     1321 gaatatccca ataacggctt taaaacaata gatgatgctc gaaaatggac gaaagagttt
     1381 gtcgattggt ataacgattc tcatttgcat agtgggattc aatttgtgtc tcctgcagcg
     1441 tgtcatgaag gacgacatct cgctatttta gagaaacgaa agcaggttta cgaggacgct
     1501 aaaaagcgcc atcctgaacg ctggtcaaaa ggtgttcgaa attggactcc acatcaatcg
     1561 gtggcgttga atcctttaaa agaaacagaa agttaacagg agcataatct ttcaagatta
     1621 tcaaataacc ctcgtcaccc aataggggaa aaagttattc ttcccctatt ggataacgag
     1681 gccaaccgac catagggagg ttctgctttt tgataggtgg aaaataaaaa tacgacaact
     1741 atat
//
LOCUS       DANKKG010000252         1743 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000252 DANKKG010000000
VERSION     DANKKG010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.258"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            82..1031
                     /locus_tag="PW004_002833"
                     /pseudo
     CDS             82..1031
                     /locus_tag="PW004_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300609.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
     gene            complement(1076..1709)
                     /locus_tag="PW004_002834"
                     /pseudo
     CDS             complement(1076..1709)
                     /locus_tag="PW004_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
BASE COUNT          545 a          317 c          295 g          586 t
ORIGIN      
        1 aagaggacag catatcgata ttgcttcctt ttttttgcta tttatttaag ggatagaagt
       61 ttagaaagga ctgtttataa gatgaaaaat gatatagtga cttatgcaaa cacaaaatgt
      121 gaagaaattg gcatcagtaa tttagaagag tgcttttttg acacaaatga cctgtcgcaa
      181 tttttaagaa cgggaagaac aaatgccagt aaacttgcca atgacttagt tgcaaagaac
      241 gttctattta aaatcaatac tcgtccagtg ttatttgcca tcaaagagcg ctttgaagag
      301 cactttttga ttccggtaaa agatgtatac gaaagtatat ctgaattaaa aacagacttg
      361 gagcccaatt ctacagattc cattctacaa aagatgattg ggtatgatat gagcttatcc
      421 gatgcggtgg aacaaatgaa aactgctgtc ttctatcctg gaagagggct tcctatgatg
      481 atcactggag atactggagt tggaaaaagt ttttttgttc atactgctta tcagtttatg
      541 aaaaaagtga acgttattca tgagaatgct ccttttaaag tacttaattg tgcgcaatac
      601 cataataatc ctgaattatt gtcggggata ctatttggct atgttaaagg tgcttttacc
      661 ggcgcagatg aagatcgaaa tggattgttg caagatgcag acggtggagt actgttttta
      721 gacgaagtac acagattgcc tcctgaaggg caagagaaac tatttgttta tatggatggc
      781 ggaacttatt ctagagtcgg tgaaactatc catcacgcct ccaatgtacg tttagttttt
      841 gcgactacag aaggcaaaga ggattttctg gaaaccttta tgcgaagaat ccctatacat
      901 atccatatcc caaatattga ggaacgaggt attttagaaa aaaacaaatt atcgaaaata
      961 ctctctttag cgaaagcaaa aactttagca aggctattga tgtttctcct aaagtcacta
     1021 gtgttgcata aatatagtca gaatattctg aattattatt ttcttgaaaa aagtaaaaaa
     1081 aatcgtatcc caaactttca tctacatttc cagaaagatc ttcatgttga taatcaattc
     1141 ctgaatcaat gaccgctacc ttcacatcct tagaaccctt ggtttcttcc caagcggatg
     1201 gcatgctaat ctctcttaaa ttagcttgtt tactccaatt aggatcgtta tcgatttcct
     1261 aaagaatcag ttgtatttgg ctcctcaaca tcattcaacg gctctagttc aaccacatag
     1321 tttggctctg cataattgac catatcagaa gcattaaatg ttcttactgc ctcttccaca
     1381 ctaatttctt cgttcaattt cactaataat acttgatctc gcttattata atcgctttca
     1441 tttgtcctaa gagtcaaatc tttttccacc ataactttta cacccaataa attttgcggt
     1501 ttatttttct ctgttgaaat tctttgtgta gcctttaaac tgacaattag ctcattgggt
     1561 acatactcta tttgagagtc atctaattgg ctcataatcc tttctttttg gttagtatct
     1621 gcagttactt gtttagtatg agccatgaac ccaaacatac tcaccccaac aaccaatcct
     1681 aaatatattt tctttttaaa ctttttcatc tacatctcct cctaatagtt ttattttaga
     1741 gtt
//
LOCUS       DANKKG010000253         1677 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000253 DANKKG010000000
VERSION     DANKKG010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.259"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            64..972
                     /locus_tag="PW004_002835"
     CDS             64..972
                     /locus_tag="PW004_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HDL2727749.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            972..1589
                     /locus_tag="PW004_002836"
     CDS             972..1589
                     /locus_tag="PW004_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727750.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
BASE COUNT          620 a          226 c          348 g          483 t
ORIGIN      
        1 cagtattata gtgcaagtgc aatttcttcc attgtcatac caccaaaata gggaggtgat
       61 ttaatggcaa agtggaatgt catattaagt acaacagaac cctataatta cgtggggatg
      121 attcaagttc gacaaggcaa taaaaacact gaagttatgg aagctacaat tgtcgaaaat
      181 ggtcttccat atgatttatc agaatgtaag gtatattttg aatcagttgt tggcggaaaa
      241 tatccagtcc aattagaaac aaaaattgtg gatgctaaaa aagggaaaat caaatatatt
      301 tttgataaat attccatgca gtgtttgcat cgacaaacag ccaatttcat tatatttaaa
      361 ggagaagact tgattggaac aactcaagac ttctcttatt ttgtcatcaa tgctgtttca
      421 aaaacagaag gagaaatggg ttcttattgg caatcaattg aagatttaat tgctgatatg
      481 acagatttta ttaatgaaaa taaaggcgat tttacggact ggatgaatga aaggaaagaa
      541 gaatttgatc gctggagaga ggaacaggag aatagtttcc aagattggag agaaggacaa
      601 gaatcagatt acttgtcatg gtttgaatca atcaaggata ttttgaagtc tatcgatcca
      661 ggtggagtaa tgttagccga attaatggat gcacgtgtag atcttcaagg tgtacgccat
      721 gaatcgattt ctgaacgttt cctatccgat ctaaattatt tgtatcaaaa aatgaaagca
      781 gcacttttca cgattgaata cggtgaaatt gaagtgactg atattcttca agacgatctc
      841 ttttcagata atcacgaagt cgaaaaaatt gaaacagtag aatttccaat tgaagaagga
      901 gctttgatta ttgcaactgt tgatgatcca aaacagaatg ttttcactct tgagaaagtt
      961 ggggtgattt gatggttaaa gtaaagagaa tgatggaaac tgatgaatct ggtgttaaac
     1021 gtcaatttat gccaattaca catgtctctg cagttttagg cttagaaaaa atgatatcag
     1081 gacaatcaaa agttctttcg gttaatggta aatatggtgc agtcatttta acgaaagctg
     1141 atttaggatt agagaatgca attactgaac taccttatgc aagcgagaca agcgatggta
     1201 ttcttactgc tgaaatgttt caaaagatcg taaatggaga aggtggaaca tatattttgc
     1261 cgattgctac tcctgaacaa ttaggaggta taaaagttgg ggaactctta gaagttactg
     1321 aagaaggtgt tttatctgca actaagcaaa cagacttcaa tttctctgaa gaattaaaag
     1381 gaaaattaga atctctaaaa atattaaaag caggtgcaaa tatatcaatt gcagaagatg
     1441 gaacaattag ttctacaggt ggtagtggga ctggtggtgt taatcagtca tatgtagatc
     1501 aaaaattcca agaagctgta aatcaagcag aaaattacac aaacgaaaga attccaaatt
     1561 ttacttttga aaaaattggg gaggtataga gaatgacaga tatcgtaaaa ataaaacaaa
     1621 gcaatgtaca ggtttatcca caaactcatt ggaatgctat agaaggcaaa ccaacaa
//
LOCUS       DANKKG010000254         1670 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000254 DANKKG010000000
VERSION     DANKKG010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.260"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            39..230
                     /locus_tag="PW004_002837"
     CDS             39..230
                     /locus_tag="PW004_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2727751.1"
                     /translation="MNTLKEDFILAKNGNEEAVVAILKRFSALVHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            744..1076
                     /locus_tag="PW004_002838"
     CDS             744..1076
                     /locus_tag="PW004_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2727752.1"
                     /translation="MKKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            986..1189
                     /locus_tag="PW004_002839"
     CDS             986..1189
                     /locus_tag="PW004_002839"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDL2727753.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKMLNR"
     gene            1373..1528
                     /locus_tag="PW004_002840"
     CDS             1373..1528
                     /locus_tag="PW004_002840"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HDL2727754.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLTDHHITHSMSQTGTPVDNAVIE
                     AFHRSIK"
BASE COUNT          612 a          276 c          293 g          489 t
ORIGIN      
        1 ataaatagat tataagttca tgggaagaag gtaaatttat gaatacttta aaagaagatt
       61 tcattttagc aaagaatgga aatgaagaag cagttgtggc cattctcaaa agatttagcg
      121 ctttggtcca taagcaatcc tggagaactg gaaaatatga tcaagattgt tatcaagaat
      181 gtatgcttgc aatatatcta gctatctcga aattcgaaat taaagaataa ctcaactaag
      241 ggagtctgtg acataagtaa aaagatagtt tcaaatttcg aacaataaat ttgaagccat
      301 cttctttata attataaagt atcgatttta cagcgttcta taatgattca tagaatctaa
      361 aacgaatgat tttaattatt aacgtttttt aattcgtttt tagcttaaaa ataacttttg
      421 tcacagtctc actcctaaaa ttactttatt tacgtacgaa gtgggcagga ggtcgaatcc
      481 agaactgggc catggtactt caacgaggac ttctcctcta gaattgaaaa gcatgcgatt
      541 taccttccgt aacgacggta gatttattca actatgtgtc caatcaaaat ttgtctgatt
      601 tcaaaaatag tcaagagaat tttggttgtt aaaaactctt atctatcaac ttccacagtt
      661 tagttgacaa accctgatac taagttgggc tatgatacac ttttcttgac tatcagttac
      721 gttaccggaa ggaatgaact ctattgaaaa aaacatatga tcgtaaattt aaactaaaga
      781 tttcacagga tatccttgag aaaaaagtaa ctaccaagaa aattgctgaa gaatacaata
      841 tctctcgtcc aacaatttcc cgatgggttt cagaatatcg tcgatacggg aaaaatgctt
      901 ttgctggaca aggaaaaaga ttacctgata aagcagattt ctacattctt gaacaggaaa
      961 ataaacgact aaaagaggaa gacgagtgtt ctacgaacac aaagatcgat acggatctcc
     1021 agggatcact caaaaacttt acgatgaagg aatcgaaatc aataaacgtg ttgtagctgt
     1081 tcttatgcgt gaaatgaatt tatgcacaag aggatttcat cgacgcaaat cttctcatgg
     1141 tagagaaaaa acaatcaaag aaatggtaaa aaaaatgttg aatcgataat ttgaacagaa
     1201 ccaaattgat gctgtttggg tgacggatat tacttacatt ccatgtaatg acggtcgcct
     1261 ctatttgtcc acctatattg acttagcaac tcgtattcta cgttactata tggtggattc
     1321 acacatgaaa aaagagattg tcgtccgtcc attacaaatt tattaaggag aattgcctac
     1381 ggtaattcat tccgatagag gtagccagca tcggtctttt atttatcaag agctgttgac
     1441 cgaccaccac atcacacatt caatgagcca aactgggact cctgttgaca atgcagtaat
     1501 tgaagcgttt catcgatcaa tcaaatgaga attaatcgac tccaacaaat ataaaagtaa
     1561 aatagagatg aaggtgttaa tccaagatta tttagaagac tactacccaa acaaaagaat
     1621 ccatacaaaa tttatgatga ctccttgaca atttgaagcg aagaaaataa
//
LOCUS       DANKKG010000255         1664 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000255 DANKKG010000000
VERSION     DANKKG010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.261"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            131..505
                     /locus_tag="PW004_002841"
     CDS             131..505
                     /locus_tag="PW004_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HDL2727755.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="PW004_002842"
     CDS             complement(619..1248)
                     /locus_tag="PW004_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL2727756.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="PW004_002843"
     tRNA            complement(1382..1454)
                     /locus_tag="PW004_002843"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          584 a          240 c          283 g          557 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt actt
//
LOCUS       DANKKG010000256         1654 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000256 DANKKG010000000
VERSION     DANKKG010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.262"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..1144
                     /locus_tag="PW004_002844"
     CDS             <1..1144
                     /locus_tag="PW004_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL2727757.1"
                     /translation="NHEVKTIDADKKTVTVTSASDNETKEYPYDKLILSSGVKPNNLP
                     VPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVGAGYIGIEAAEASRKAGKEVVL
                     LDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKIEAFTGNGKVEAIKTSEKEIPA
                     DLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTNLPDVYAVGDATLAYSIPARKK
                     MPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSALSVFDYHFAASGLNSFTAAKA
                     GVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMTHQLLGGAVLSTYDVTGQGNVL
                     ALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQALGEEPFVE"
     gene            1425..1654
                     /locus_tag="PW004_002845"
                     /pseudo
     CDS             1425..1654
                     /locus_tag="PW004_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          591 a          293 c          356 g          414 t
ORIGIN      
        1 taatcacgaa gtaaaaacga tcgatgccga taaaaaaaca gtaacggtta ctagcgcatc
       61 agataacgaa acaaaagaat atccttatga caaattgatt ttgagttctg gtgtcaaacc
      121 aaacaatctg ccagtaccag gagcagatct agaaaatgtc tacttgatgc gtggatacaa
      181 ttgggcaacc aaaatcaaag aaaaattaaa tgatccggct attaaaaaaa tcgctgtcgt
      241 aggtgcgggt tacattggga tcgaagcagc agaagcaagc cgaaaagctg ggaaagaagt
      301 cgtattgctt gatgtgatcg atagaccttt aggcacttat ttagatgctg agatgacaga
      361 tattttagaa caacacctaa atgaaaacgg tattgaagtt ttgacaaatg caaaaatcga
      421 agcttttact ggtaacggaa aagttgaggc aatcaaaaca agtgaaaaag aaatcccagc
      481 agatctagtc attcaagctg ccggtgtgaa ggcaaataca gaatggttaa aagggatcgt
      541 cgacttagat gaacgtggct ggatccaaac aaatgaatac ttgcaaacga atcttccaga
      601 tgtgtatgca gtaggagatg ctacacttgc ttattctatc ccagcacgca aaaaaatgcc
      661 gatcgcttta gcgactgttg cacgtagaga agcacgttat gtagtaaaac atctttttga
      721 agaagtacca aacaaaccgt tcggcggagt cgtaggttcc tctgcattaa gtgtctttga
      781 ctatcatttt gcagcaagcg gattgaatag ctttacagca gcaaaagcag gagtagatgt
      841 acgagtgtct tattatgaag atacgttacg accaaaatat gtaccagaag aacaaggaaa
      901 tccaaaagtg tccatccaat tattctcgga tccaatgacc catcaattgc taggaggagc
      961 agtattatct acctatgatg ttacaggtca aggaaatgta ttggcactag cgatccaaaa
     1021 caaactgact ttagaagaac tagcagaagc tgacttcttc ttccagccag gctttgaccg
     1081 acaatggagc atgttgaatc tagccgctca acaagcgtta ggagaagaac catttgtaga
     1141 ataagaaaac gaaatatttg aaaagaggtt gtgaaaaagc tgccttgcat caagtaataa
     1201 gaacagccaa gtaaaaacag cttcttatgt ttttaacttg gctggactta ttaccgcaga
     1261 tgcaagcttt tgaacaccgt ttattttcaa aaaagggact gtgacacgat ttgtgtaccg
     1321 acccccaaaa gttagaccaa aaaatctaac ttttggaggt cggtattttt tatggttaaa
     1381 tatacttttg aacttaaatt aaagatcgtt catgattatt tggatggcaa aggcgattgg
     1441 accgtaaaaa tccagatcaa gagattgaag cggaaatgct taaaattcgg gagaagcaca
     1501 aggattatgg ctgtttacgg atgaccaatg aactaagaaa ccgcggtttc tctatcaata
     1561 aaaagaaagt tcagcgcttg atcaagaagc taggaataaa agtaacggca tacacgcgta
     1621 aatcacgtcg ctacaattct tatcgaggaa aagt
//
LOCUS       DANKKG010000257         1647 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000257 DANKKG010000000
VERSION     DANKKG010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.263"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            188..784
                     /locus_tag="PW004_002846"
     CDS             188..784
                     /locus_tag="PW004_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhK family protein"
                     /protein_id="HDL2727758.1"
                     /translation="MKTVKKMTKLVFASLGIIVFLGACSNQSESNNSNSTNEESTSTA
                     SSEMNSMEGMNHEGMVPSSMKDAANPKFPVGSNVILLGDHMEGMKGANAQVVGAFDTT
                     MYEVSYEPKTGGPMVNNHRWVVQEELKDTETVANEGDTVILNADHMDGMMGAEAKVDK
                     SITGTVYVVNYTPTDGQEEVKNHMWVTEDEMEYDENNE"
BASE COUNT          590 a          222 c          310 g          525 t
ORIGIN      
        1 aatgttgggc aaaaaaaata ttttttcaac tgatttcgat agatagtgtc gaaaaagttc
       61 atttagatat cttatcaaat tctgtagtaa tcttttctaa gaaggactta tcaatagcta
      121 caattaaaaa agaagttttt ggattacaaa tttatgagat gacctaaatg tgattaatta
      181 aaggaggatg aagacagtga aaaagatgac aaaattagta tttgcaagtc ttggaattat
      241 tgtattttta ggagcgtgta gcaatcaaag tgaatcgaat aattctaata gcacaaacga
      301 ggaatcgact tctacagcgt ctagtgaaat gaatagtatg gagggaatga atcacgaagg
      361 tatggttcct tcttcaatga aagatgctgc gaaccctaaa ttcccggtgg gaagtaatgt
      421 tatactgcta ggcgatcata tggaagggat gaaaggtgca aatgctcaag ttgtaggggc
      481 ctttgatact acaatgtatg aagtaagcta tgagccaaaa actgggggac caatggttaa
      541 caatcatcga tgggtagtac aagaagagtt aaaagatact gagactgtag caaatgaggg
      601 cgatactgtt atcttgaatg ccgatcacat ggatgggatg atgggagctg aagcaaaagt
      661 agataaatct attacaggaa ctgtttacgt tgttaattac actccaaccg atggtcaaga
      721 agaagttaag aatcatatgt gggtaactga agatgagatg gaatatgacg aaaataatga
      781 gtaattttat atactaaacc aacaagctgg tacatggtct tcgtattctc aacatgagga
      841 atatgagctc tttgttaata aggactgatg agttgtgcaa aatcaaatct gggtcagaac
      901 gaacctcatt ctggctcaga tttttggctc ttcgacattt agtgctggta gagagacaac
      961 tgaaaattta gtcataacca gttaggcaga gtgcttaact ggtttttgtt cgcatttatt
     1021 attcttttga tattggctgc ttgcccttca attttatttg gtttcttaat caactcttca
     1081 tataacccca aatcatatat taatgaggtg cctcctattc ctataaatct atctcttctt
     1141 taatagtatg agaaaagtct actcttgtag atttaaattg tcaacaaaaa atttacagat
     1201 gtaaatgata agttgaatag agaaagataa attatttttt tggtattcat ataaggttaa
     1261 ttatgtatta tcacaaaatg attgttgtcg aataactaaa agtgtcaaaa caatgatgaa
     1321 atcatttagt tcttataaat tagacaagag aaaataaata ttccatatct actttattgg
     1381 atccataaca tgactaaatt ctgaatactt tttaaagctt atcatttggt aaaactgaat
     1441 ataagttaat tatatagact gaaaattttg aattttcaga atagaataac ttaaaagtac
     1501 tgacaaattt tttaaagggg caatattaat gttctaagat aatcaaatct taccgaaaat
     1561 atcaaaagtc caatgtgaca caattggatt ttgtcacatt cgttaaaatc gctccatcct
     1621 tagaggctct aataggaaat cactttt
//
LOCUS       DANKKG010000258         1583 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000258 DANKKG010000000
VERSION     DANKKG010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.264"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(<1..656)
                     /locus_tag="PW004_002847"
     CDS             complement(<1..656)
                     /locus_tag="PW004_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727759.1"
                     /translation="MEFINNTKEKILNSPYFNKVSYLFEESFMTYSIGAYRSSYITSY
                     VGFLQQIRQNIINYNENPYFSIIEKKENETEEKYKAKIENKWRGLNKNLIDVDKWENA
                     LISALNENTDTNILRLNDGDRTKLLYFKNIRNDAVHDKTNQITLAQLQSLWEFIIEYA
                     HRTRIGENKESFLKNYQEITEWYKQSGDIPENRLRVIEESFYLLTEKEKIEILSIFYR
                     "
     gene            complement(757..1368)
                     /locus_tag="PW004_002848"
     CDS             complement(757..1368)
                     /locus_tag="PW004_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2727760.1"
                     /translation="MAKIGYARVSSIDQNLDRQLELLSKCDKIFTDKASGKDTNREGF
                     QKMMEFIREGDIVVVTELKRLGRNNKELTETMNLIQLKKATLEVLNLPALTGITDDNL
                     RRLLNNLIIELYKYQAEEERRYIRETQQQGIALAKAKGKYKGGKPKYREDDKRLQLAF
                     KLFLAGSTDKEVEEQTGINRRTFRRYRMKYGITSKLREKNKIK"
BASE COUNT          458 a          291 c          177 g          657 t
ORIGIN      
        1 cttctataaa aaatactaag aatttctatc ttttcttttt ctgttaataa ataaaaagac
       61 tcttcgatta ctcttaatct attttcagga atatccccag attgcttata ccattcggta
      121 atttcttggt aattttttaa aaagctttcc ttgttttcgc ctattctagt cctatgtgca
      181 tattctatta tgaattccca taaagattgc aattgtgcta aagtgatttg atttgtttta
      241 tcatgcactg catcatttct aatattttta aagtataaaa gcttagttct atccccatcg
      301 ttcagcctta atatattagt atcggtattt tcatttaaag cactaataag agcattttcc
      361 catttatcaa catcgattag atttttgttt aaccctctcc acttgttttc aatctttgct
      421 ttatattttt cttctgtctc attttctttc ttttctatta tactgaaata aggattttca
      481 ttataattta ttatattttg tcttatttgt tgtaaaaagc ctacatacga agtaatgtac
      541 gatgatctat acgctcctat tgaataggtc ataaaagact cttcaaataa ataagacacc
      601 ttattaaagt agggactatt caaaattttt tctttagtat tgtttataaa ttccatttac
      661 tccatcctta cttaattttt aaattaatgg attatatttg ttaattaaga ctcaagtaaa
      721 aatatgaaat cggttcaatc tctgtgaatt tttaagttat tttatcttat ttttctctct
      781 caatttacta gtgattccgt atttcattct gtaccttcta aatgttcttc gattaattcc
      841 tgtttgctct tctacttctt tatctgtaga tccagctaaa aataatttaa atgctaattg
      901 cagacgttta tcatcttctc tatactttgg cttcccacct ttatattttc cttttgcttt
      961 agcaagcgca ataccttgtt gctgcgtttc acgaatatat cgacgttctt cttccgcctg
     1021 gtacttgtat agttcaataa ttaagttatt aagtaatcga cgcaaattat catcagttat
     1081 ccctgttaac gctggtaaat tcaaaacttc cagagtcgct ttcttcagct gaatcaaatt
     1141 catcgtttct gttagctctt tattatttcg cccaagacgt tttaattctg tcacaacgac
     1201 aatatcacct tcacgtatga attccatcat tttttgaaat ccttcacgat tggtatcttt
     1261 cccgcttgcc ttatcagtaa aaattttatc acactttgat aacaattcta gctgtctatc
     1321 taaattttga tcaatactgc ttactcgagc atatccaatt ttagccatag aaaaaaactc
     1381 ctttaaaatt caaaaatttg gatcattttt aaaatcctgt tgcccaactc tgttgacccc
     1441 ttagaaatcc aagtgtcatt tttatattta aatggcaaaa atttttctca gtatttgtca
     1501 cacaaaaatt cgaaaaatat tctatagctt tatagaaggg ccaacaactt ttaaggtttt
     1561 gatcctgttt taatttctca ttt
//
LOCUS       DANKKG010000259         1573 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000259 DANKKG010000000
VERSION     DANKKG010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.265"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
BASE COUNT          594 a          179 c          320 g          480 t
ORIGIN      
        1 attatcgaac acctttggaa gtatttttga gttacgtaag tattgatgat ctgtctaatt
       61 taatttgaca attaagaaag tattaacttg ttttgtgaaa aagagaattt gaaaaacgat
      121 tggcgaacaa atgctcggta ggtatggtat aaatacagag ctaattggtg tgatgaaatc
      181 agatattcgc gtgagattca gtaaagtagg agttcgaaaa cagctaggtg acaaacagta
      241 ttgctttggt acttcggaat ggtttatcaa attcttatca acagttcact tctctttatg
      301 tttgagtgat gattcgttag gttattgctt ggaaagaccg gatgagagta gaagagttta
      361 attttataaa gtgaaaagag gtaatgtacg atatacgtag ggctgaacca atagaggata
      421 agcaaacgat gttacaggcg caaaattcat agcttactgt tttggagata acagaagagg
      481 aaataatgca gaagtaaggc gaaaaaagga cggaagccta acagcatatg aaataaagaa
      541 aaatattata actactgata aatcggtatt gagcaaagta ctaataggag tcaaaaacat
      601 tcataagcta tgagtgtttc ttggctactt ttttattttt atgtatctac aaaagtttcc
      661 aggggcagaa cctgtgatta ttaatggaag aacctcaagt attgtcttaa atcattgtta
      721 tgaaaatgga ttaaaagagt tcatcaaggt gtgattaaca agttactttt cattacatcg
      781 aatttttcag aagttgctta tattggtgat gatattaatg atttatcatg tatgacatct
      841 gtgaagaagc gggaggaata gtagaatgtc caaaaaatgc cacaagaaat gagattgaat
      901 tatctgattt tattgtaact cacagtggtg gtgacgaatc tgtgatagag tttattgagt
      961 ggatattgga atcgatatag tgtagtagta cagaaaaatt atcatacaat taatactagg
     1021 cataagaata ttggcatatt ctagagttaa aagtaactat tcgaaaagga taaggttaga
     1081 attttttgtt ttgccagtgg aagaatataa aaagggcatt ggaaattaat atattagaat
     1141 gctaagcgga gaaaaaaata tatgaaaagt acgtccttgt gatgggatgg aaaaatacac
     1201 ttatttcaaa caacgataat ataattatgt gaaccgaagt atactcgctt ctaattcaat
     1261 cctcaaatcc cgacagtcgc gacttcaaaa aagcgtatag ttttaatgta tccccatatg
     1321 atccgaaaaa tggtaattta caattaacag tttgatgatg ttgaagatgt ggaaatgctg
     1381 aaattaccat gatgagaaac acattaatta aagggaatta attaactaga atataatctg
     1441 attcaatttg aattaagcaa aaaaaggctg agttttaaat aatcgcgaat tgtaaaatta
     1501 agttagaaaa ataaaaaggc atttatggta cactcaaatt gtatttccga cgaaagaaaa
     1561 caaaggagtg taa
//
LOCUS       DANKKG010000260         1549 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000260 DANKKG010000000
VERSION     DANKKG010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.266"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            186..1475
                     /locus_tag="PW004_002849"
     CDS             186..1475
                     /locus_tag="PW004_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL2727761.1"
                     /translation="MRYSTILISTFAALSLSLTINQTVHTEEIETAKWTETKWANVNR
                     ITFQDEFSSFNSGVAKLRKGQTATIKAKIDYSGDLAPILQSAVTFENVDPALSIGYDE
                     NTLTIQHKQAIFTFTVTLNQDLLKPALFTVKVSDGLPNDHHHLTPYSQRQTIEQDSAI
                     DSGGDLVEEPTDKPENENKPEVPPTENPDGEQKPEIEPGEEPDTETQPEPDNESKPEI
                     TPGEKPDVDPEEKPDVTPEPDTDSGNQTVPETNPDTDNETENPEKPEVAPEEKPDVTP
                     EPDTDSGNQTVPETNPDTDNETENPEKLEVDPEEKPDVTPEPDTDARDQGIPEKINKK
                     TIQEDGKKESKKSNLAILKINEEQLNKKSRIFDSAQSAETLKSSKDTTFASPETKNKQ
                     LPKSGESQNKVILWSGIILLSIATMLSAKRFKQNRSL"
BASE COUNT          647 a          308 c          269 g          325 t
ORIGIN      
        1 ttcaagagaa acgttttaat cgcttcttga gtttgcatga aagaggctcc tttttactta
       61 aattgttaaa acttgaaaat attactgttt ttttcataaa tttagacaaa aaaacagtaa
      121 acatataggt agaatctcta ctatcttatt tcttgcatac aaaaaaatca aggagtgaga
      181 gagttatgag atattcaacc atcttaatat caacatttgc agccttaagc ttatcgctta
      241 ctataaatca gacagtccac acagaggaga tagaaacagc caaatggaca gaaaccaagt
      301 gggccaacgt caatcgaatt acctttcagg atgaattttc atccttcaat tcaggagtcg
      361 caaaattaag aaaaggacaa acagcgacca tcaaagcaaa aattgactat tcaggagact
      421 tagcacctat actacaatca gctgttacat ttgagaatgt tgatcccgct ttgtcgattg
      481 gctatgacga gaacactttg actatccaac acaagcaagc gatatttacc ttcaccgtta
      541 cattaaatca ggacttgtta aaaccagcgc tttttactgt caaggtatca gatggactac
      601 cgaacgatca tcatcattta acgccgtata gtcagcggca gacaattgag caagattcag
      661 ctatcgactc aggaggagat cttgttgaag aaccaacaga taaaccagaa aatgaaaata
      721 agccagaagt accgcctaca gaaaatccag acggagaaca gaaaccggaa atagaacctg
      781 gagaagaacc cgatacagaa actcaaccag agccagacaa tgaatcaaaa cctgaaataa
      841 cccctggaga aaaaccagat gtagacccag aagaaaaacc ggatgtgact ccagaaccag
      901 atacggattc tggaaaccaa acagtaccgg aaacaaatcc agacacagat aatgaaactg
      961 aaaatccaga aaaaccagag gtagccccag aagaaaaacc agatgtaact ccagaaccag
     1021 atacggattc tggaaaccaa acagtaccgg aaacaaatcc agacacagat aatgaaactg
     1081 aaaatccaga aaaactagag gtagatccag aagaaaaacc agatgtgact ccagaaccag
     1141 atacggacgc aagagaccaa ggaataccag aaaaaataaa caaaaaaaca attcaagagg
     1201 atggaaaaaa agagagtaaa aaatcaaatc tagcaattct aaaaataaat gaagagcagc
     1261 tgaataaaaa atcacgaata tttgatagcg cccaatcagc tgaaacctta aaaagtagca
     1321 aggatacaac ctttgctagt cctgaaacga aaaataaaca gctaccaaaa tccggagagt
     1381 cacaaaacaa agttatactg tggagcggaa tcattctatt aagtatagca acaatgcttt
     1441 ctgcaaaaag atttaaacaa aacagatctc tctaacattt gaataatgtc tgtgaacgca
     1501 tttttcaaat aaaaagaaat atccagaaaa cgaccccaaa ataccagat
//
LOCUS       DANKKG010000261         1526 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000261 DANKKG010000000
VERSION     DANKKG010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.267"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(<1..734)
                     /locus_tag="PW004_002850"
     CDS             complement(<1..734)
                     /locus_tag="PW004_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HDL2727762.1"
                     /translation="MKKKIFVGAIIALFLLPINAFAYTINNEFNLGANEGSSQVANNQ
                     YILLHETANETATGRNEAQYMKRSWYNAYTAYIVGDGGIVYQVGQPGYVQYGAGSYAN
                     ANSPVQIELQHTHDKVTFEKNYKAYVELARDSAMKYGIPLTLETPYNQPGIKSHLWVT
                     QNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSDDKPVIDPTRAGAANPTL
                     TDGTNYAHIDQFGEIENANLHVAGWH"
     gene            complement(731..1150)
                     /locus_tag="PW004_002851"
     CDS             complement(731..1150)
                     /locus_tag="PW004_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDL2727763.1"
                     /translation="MEKYLNTFSVMTGVIGGTIVGLLGGMDNILHVLIFLVGVDFLTG
                     LAKAWKLKEISSEIGFEGLLKKVLIFVVIAVAVEVQKIVGNSIPLREIVIMFYVANEG
                     ISFLENISVFLPLPDKLKEVFQQIRNDTENKDRGGTK"
     gene            complement(1220..1360)
                     /locus_tag="PW004_002852"
     CDS             complement(1220..1360)
                     /locus_tag="PW004_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HDL2727764.1"
                     /translation="MNSFPGFENIKQFYDWGCYTDQDLLDYVNMNCLTKDQYKQITGN
                     EI"
     gene            complement(1353..>1526)
                     /locus_tag="PW004_002853"
     CDS             complement(1353..>1526)
                     /locus_tag="PW004_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727765.1"
                     /translation="SGTAVDDIEDVDQLKQLNALLTKQLAISVQEQEKMQQMLAQLTM
                     EVASIKNGGKSNE"
BASE COUNT          417 a          327 c          236 g          546 t
ORIGIN      
        1 atgtgccatc cagcgacgtg taagttcgca ttttcgattt ctccaaactg atcaatgtgg
       61 gcgtaatttg ttccatctgt cagtgtagga tttgctgcac ctgctctagt tggatcaatg
      121 actggtttat catctgaagt tgtcggattt tcatcggtaa atccatgagc taaatcatat
      181 gctaattttt ctttacttac gcccatttca gaaagataac cgtaaggatc tgtatgatcg
      241 ccccagatat tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggcgtt
      301 tctaatgtta atggaatacc atatttcatt gctgaatctc tagccaattc aacgtatgcc
      361 ttgtagttct tttcaaacgt tactttatcg tgcgtatgtt gcagttcgat ttgcacagga
      421 ctatttgcat tagcatatga accagcgcca tattgtacat aaccaggttg tccaacttgg
      481 taaacgatcc caccatcgcc aacaatatac gctgtatagg cattgtacca agaacgtttc
      541 atgtactgcg cttcatttcg tcctgtcgca gtttcgtttg ctgtttcatg caataggatg
      601 tattggttat ttgctacttg agaactacct tcgtttgcgc ccaaattgaa ttcattgttg
      661 atcgtgtaag caaaggcgtt tattggcaat aaaaaaagag ctataatagc tcctacaaag
      721 attttctttt tcatttggtt cctcctcggt ctttattttc agtatcattt cgtatttgtt
      781 ggaatacctc ttttaactta tctggcaaag gaaggaatac cgaaatgttt tctaaaaaac
      841 taatcccttc atttgcaaca tagaacataa taactatttc tcttagaggt atagaattcc
      901 caacgatttt ctgtacttca accgcaacgg caatcacaac gaagattaag actttcttca
      961 acaatccttc gaaacctatc tcactactta tttctttcaa tttccatgcc ttagcaagtc
     1021 cagttaagaa gtccacaccc acaagaaaaa ttaaaacatg tagtatattg tccatccctc
     1081 ccaataaacc aacaattgtc ccgcctatca cccctgtcat tactgagaat gtatttaaat
     1141 atttttccaa tatctatttc tcctttatgc aaaaaagcgc actcgtttga gtacgctttt
     1201 tatttaactt tgtgcttaat taaatttcat ttccagtaat ctgcttatat tggtcttttg
     1261 ttaaacagtt catgtttaca tagtcaagta aatcttggtc cgtataacat ccccaatcat
     1321 aaaattgttt tatattttca aagcccggaa aactattcat tacttttccc tccattctta
     1381 atacttgcga cttccatcgt caattgagct aacatttgtt gcattttttc ttgttcctgt
     1441 accgatatcg ccaattgttt tgtcagtaga gcatttaatt gcttcaactg atcgacatct
     1501 tcaatatcgt caaccgctgt gccact
//
LOCUS       DANKKG010000262         1517 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000262 DANKKG010000000
VERSION     DANKKG010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.268"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..1458
                     /locus_tag="PW004_002854"
     CDS             <1..1458
                     /locus_tag="PW004_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2727766.1"
                     /translation="ITTQYLADHFHMSESNFLLYIKELEQDFERLNLTELHIDKQKPF
                     LKLNFEGIDPAYCYYRLFGRYCNESVSYQILTSLFSCQTNSIISLSQQTNYSASYLYT
                     KMKKINAFLALYGLSISFSNNGRKSFSGKEVQIQYAALDIYWNIFSSTATEFYDEDPQ
                     VVAFMMNTFFKKEVLDRLNSGMIDKLYFLLKICKENFPATSVKDVQKEFTEYEYIDFF
                     IDPSVDILRPNLPICREQRILVNILARLSITKIESEETSLEQYKLLKKANVPHVLYSR
                     EMVESFCTVFDLFIPEKEKILYSLAFARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLK
                     EVQTAIEQFYMDFREQNQHLLPVILEKENVQWMVEDLVHLYDRYKKQPRIVIGVNYTR
                     DFYISKDLIVKIEQIFSNESIKIQNDFMENCNIVISDCPLKQLPSHIKKIYILKGIIT
                     PEDWKKVIMRISEYIFELKEEALMDQSKKENQVTS"
BASE COUNT          544 a          247 c          240 g          486 t
ORIGIN      
        1 attactacac aatatttagc agatcatttc catatgtctg aatcaaattt tttactatac
       61 atcaaagaat tagaacaaga ctttgagcgt ctcaatctaa cagaactgca tattgataag
      121 caaaaacctt ttttaaagtt gaattttgaa ggaattgatc cggcttactg ctattatcgt
      181 ctatttggaa gatactgcaa cgaatcagtc agttatcaga tcttgacttc tttgttcagc
      241 tgtcagacaa attcgattat cagtttaagt cagcaaacaa actatagtgc ctcttatttg
      301 tatacgaaaa tgaaaaagat taatgcattt ttagcattgt acggtttgtc tatctccttt
      361 tctaataatg gcagaaagtc tttttctgga aaagaggtac aaattcaata tgcagcatta
      421 gatatttatt ggaacatctt ttcaagtact gctacagaat tttatgatga agatccgcaa
      481 gtagtggctt ttatgatgaa tacttttttt aaaaaagaag tacttgaccg actaaatagc
      541 ggtatgatag ataaattata ttttctatta aaaatatgta aagagaattt tcctgcaact
      601 tctgtgaaag acgtacaaaa agaattcaca gagtatgaat atattgactt ttttattgat
      661 ccctctgtag atattttgcg acctaatctg ccaatttgtc gcgaacaacg catcttagtg
      721 aatattttag ctcgactttc tatcacgaaa atcgaatcgg aagaaacaag cttggagcaa
      781 tacaaacttt taaaaaaagc aaacgttcct catgttcttt attcaagaga aatggtggag
      841 tccttttgta ctgtatttga tttgtttata cctgaaaaag aaaagattct ttactcattg
      901 gcattcgcac gaaataaact ttataatgga tttcttaact tcaatcaacc aaatacgccc
      961 cttcctactt tcatgcttta tcaagaacat agcgtgttga aagaagtcca aaccgccatt
     1021 gaacaatttt atatggattt cagagaacaa aatcaacact tgttaccagt tattttagaa
     1081 aaggaaaatg tgcaatggat ggttgaagac ctagtacatt tgtatgatcg ctacaaaaaa
     1141 cagccgcgaa ttgtgattgg tgtgaactat acgcgtgatt tttatatcag taaagatctg
     1201 atcgttaaga tagaacaaat tttttcaaat gaatcgatca aaatacaaaa tgattttatg
     1261 gagaactgca acatcgttat cagcgactgc cctttgaaac aattgccttc tcatatcaaa
     1321 aaaatctata ttttaaaagg aatcatcaca ccagaagact ggaaaaaagt gattatgagg
     1381 atctcagaat atatctttga attaaaagaa gaagcactca tggatcaatc gaaaaaagaa
     1441 aatcaagtta cttcctagac caaataagat acataaatcc gaatgcttat taacaacctc
     1501 cggatttatg tatcaag
//
LOCUS       DANKKG010000263         1492 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000263 DANKKG010000000
VERSION     DANKKG010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1492
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.269"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..1448
                     /locus_tag="PW004_002855"
     CDS             <1..1448
                     /locus_tag="PW004_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HDL2727767.1"
                     /translation="GFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDTVANGGLG
                     LPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIVLALPFGV
                     STLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGALLAPLIV
                     GTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEERKGFIRNL
                     IIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVTAEEKPRV
                     WAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNPIFIFILT
                     PVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPLWLLGSFF
                     IVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFYTPGTESA
                     YFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          403 a          286 c          315 g          488 t
ORIGIN      
        1 agggcttctt cgggcagccg aagggattgt caactttgtt cttcactgag atgtgggaac
       61 gatttagtta ttacggtatg cgtgcattac ttatctatta tatgtacgat acagttgcca
      121 acgggggact tggattacca cgagcaacag cattagctat catgtcgatc tacggatctt
      181 tgatctacat gtctagtatc gtcggcggat gggtatctga ccgagtgtta ggagcaaaac
      241 gcactgtatt cttcggtgga gttttcatta tgcttggcca catcgtcttg gctttaccat
      301 ttggtgtaag tacattgttt gtttcgatgg cattgattat tatcggaacc gcctttttga
      361 aaccaaatgt ttctggcatg gtcggacatc tttattcaaa gactgacttg cgaagagatg
      421 caggattttc gattttttac atgggtgtca acttaggggc attattagca ccactaatcg
      481 ttggtacaat cggtcaagaa tataactatc atttagggtt ctctttagct gctattggga
      541 tgttctttgg attattacaa tacatcatcc aaggacgcaa gacattagac ggagtgggta
      601 caacaccgcc gaatcctttg acaactgaag aacgtaaagg attcatccgt aacttgatca
      661 tcgcacttgt tgttatcgct gcagtattcg gcggggcaca actaactggt catctgacaa
      721 ttgacttttt tgtcaataca atcagtgtat tggggattat cttaccgttg tattacttta
      781 tcaaaatgct gacttcaaaa gatgtaactg ctgaagaaaa accaagagta tgggcttata
      841 tcccattatt ccttgcagca atcattttct ggtcattaca agaacaaggt tcatctatct
      901 tagctttatt tgctagtgaa cgtacacaat ctacgctatt tggtgtaacg atccaagcaa
      961 gctggtatca atcattgaat ccaattttca tctttattct gacaccagta ttcgtaacga
     1021 tgtggacgaa acttggtgaa cgtcaaccat ctacagtagt gaaattctct cttggacttt
     1081 tatttgcagg attatcgtac ttgctattga tgttgccggg tatgctttat ggtacaagtg
     1141 gaaaagtaag tccattgtgg ttgttgggaa gtttcttcat cgtggttatt gctgaattgt
     1201 gtctttcacc agttggtttg tctgtgacaa caaaattagc accacgagca tttgaatcac
     1261 aaacaatcgc tatttggttc ttagctgatg cttcttcaca agcaatcaat gcacaaattg
     1321 cacgcttcta cacaccggga acagaatctg cttacttcgg tattatcggt gcagtagcaa
     1381 tcgtcggtgg tatcttgcta tttactgtga aagaaccaat caaaaaacta atgggctcta
     1441 ttcattaatc aatgaacata aaaacaggaa ttgcttattt gcagcagttc ct
//
LOCUS       DANKKG010000264         1478 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000264 DANKKG010000000
VERSION     DANKKG010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1478
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.270"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            33..326
                     /locus_tag="PW004_002856"
     CDS             33..326
                     /locus_tag="PW004_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727768.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            319..615
                     /locus_tag="PW004_002857"
     CDS             319..615
                     /locus_tag="PW004_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727769.1"
                     /translation="MAKLIVLGGLIYGGYWLYQYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHTGYRHYWERID"
     gene            690..>1478
                     /locus_tag="PW004_002858"
     CDS             690..>1478
                     /locus_tag="PW004_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HDL2727770.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKATIETDTTIETKKSKTQS
                     TEQTTTEMKQSVETSASSITESSSEEVKNTLWDTNKASKLETFVTQWGKTLGQEYKSY
                     NLQNNVSLYGTPLPQAVINGDWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVETGE
                     YLGQHVYFFGFQNGQPKVYLTQQNQGNENNYLYFNETQNQQLKQGFSDIVNGQTPQTP
                     VVEESTQQATTTTTQSTSSSGISSWEAAKESVLNNKALWLYDSPD"
BASE COUNT          559 a          208 c          300 g          411 t
ORIGIN      
        1 tcattatgta aaaaataaca gggggtgtgt agatggagat tgatgaaact tgtttgagta
       61 actcttataa aagtatgaat gacttttttg aggaaatgga actggattca gaattgctct
      121 atcaatttta tcttgctttt cgagggcaac agatttcttt tccaatgaaa atgtacgatc
      181 gtgagttagt tagaaaaaga atcgaaaaca tgatagaaca ggaaaaaact gtagatatcc
      241 gtcaattgac agaagtttat ggtttctctg caagatggat tcaaaaagta attaaacaaa
      301 aagaaaggag acgagtacat ggctaagctg attgtattag gtggtttaat ttacggtgga
      361 tattggttat atcaatatat gcttgcgcat ccgaatatag ataaaggaaa agtaattaag
      421 acagttgggg taatcatagt tggactcata gctctaacta agaaaaataa tactaatagc
      481 ccaaaaataa tgagaactta tcgctgtaga ggatgtggaa gggaaatttc tactagtttt
      541 aatccatctg agagtcctca aggaagagac cgctgccata cgggctatcg acattattgg
      601 gaaagaatcg attaaaagat taccatttag atcaattaat tgtctgatag aataagtacg
      661 tgagggagaa attgaaaggg ggaaatataa tgaaaaaatg gctgttatta ctaataggaa
      721 tgggaatttt attcactggt tgtacatctg agaatacgag gactaaagcg actatagaaa
      781 ctgatacaac aattgagact aaaaaaagta aaacgcaatc aactgaacaa actacaacag
      841 aaatgaagca atctgtggaa acaagtgcaa gttcaatcac tgaatcttct tctgaagagg
      901 ttaagaatac tttgtgggat acgaataaag cttccaaatt agaaactttt gtcacacaat
      961 ggggaaagac attaggtcaa gaatataaaa gctacaatct tcagaataat gtaagtctgt
     1021 atggcactcc tttaccacaa gccgtgatta atggcgattg gaaaatggca atcaatgaag
     1081 caccagttac cgtacaatgg tcagaagatg gaactggtca tgcagatttc aatttagtag
     1141 cagtttattc tgatgtagaa actggagaat atttagggca gcatgtttac tttttcggtt
     1201 ttcaaaatgg acaacctaaa gtttatttaa cgcaacaaaa tcaaggtaat gaaaataatt
     1261 atctgtactt taacgaaact caaaatcaac agttaaaaca aggtttcagt gatattgtga
     1321 atggacaaac tcctcaaaca ccagtagtag aagaatctac tcaacaagca acaactacaa
     1381 cgacacaatc tacaagtagt tcaggaattt caagttggga agctgctaaa gaatctgtgc
     1441 taaataataa agctttatgg ttgtatgatt ctcctgat
//
LOCUS       DANKKG010000265         1472 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000265 DANKKG010000000
VERSION     DANKKG010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.271"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(139..438)
                     /locus_tag="PW004_002859"
     CDS             complement(139..438)
                     /locus_tag="PW004_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HDL2727771.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            789..1421
                     /locus_tag="PW004_002860"
     CDS             789..1421
                     /locus_tag="PW004_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDL2727772.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
BASE COUNT          512 a          231 c          204 g          525 t
ORIGIN      
        1 aggataaaat acaatataaa gaaacatctg tttctttatt tagaataaaa aaatacagac
       61 tctgttcaaa taaatgaacc tagcctgcat tatgagattg actatacttt ttaattcatt
      121 gagatgaata actcgttttt ataaatgccc aaaagtaatc ggtgccgaag ccattcctcg
      181 acctaattct ttttcaaatt ttcctattgt tgatatctcc atataaaacg gtgatactgg
      241 tttagacaat tctcccctga ttgctaacgg acgtaaacta cgttccaaat tatttatgac
      301 tttaggataa tactgacctt tttctaagcc tgtctttgcg tctatcaaaa gtatacgttc
      361 cttttctttg atctctggat ttaaaacgag atcatataat tttagtacca tgttttctat
      421 ttcttcactt ttagacatca cgatttcctc ccaaaaatat atttcttccc actacattct
      481 ataaacaaag tatacgctac atctagattt ttttaaatca ttactggatt gttaaaacct
      541 ttcaatacac tgttatggca acgattttta ttttaaaaac attctaaatt aaatcagtaa
      601 aaatttttat tctaagattt ttattagaat tacgcctaat ttgtgtctct cagggcaatt
      661 ctaagaacgt ttcaagagat agcgacacta tcgttatcga aaaaggcgaa aacctatctc
      721 attttctgct aatgtaagtt ggtttagctt ttgttaatat gactttatga aaatggaggt
      781 aattaaatat gatttttcct aaagttaatg aagcaagctt gaataaagga tccttttttc
      841 atatctatga aaacgaagat tttgaactgt ataatatcct ttcacaaaaa gaggtcatcg
      901 aaaaccaaat aatctgccat ccaattattt acaaatctat tttcaagtat gcattttttg
      961 cttccaatga ttttcaaaga atggagatta ccgactgtat attcgaaaac tgtgattttt
     1021 caggctgtat attgacaaaa agcatgttgc atcgcgtgaa atttgtcaat tgtaaattaa
     1081 caggtactaa attgatggaa agttatattg ggaacacgtt attcgagaat tgtaaaatgg
     1141 actatgtaaa tttatctggt tcaaacataa aagaaagtac ctttgagcac tctgtcttat
     1201 caagcgcaga ttttgtagac tgttccctca ctaaaataat gttttctact aatgatttag
     1261 aaaatgctag cttttttgat actgatttaa aaaatatcga tttaagtagc aactcctttg
     1321 aaatgattga agtacgctcg gaacatctaa aaaattgtac gcttaatcag ttgcaagcac
     1381 ttggttttgc tagaaaattt ttacaaataa aagtagtata atcaaaatga atatataaga
     1441 taaattttta gcaatacaaa tatccataaa aa
//
LOCUS       DANKKG010000266         1464 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000266 DANKKG010000000
VERSION     DANKKG010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.272"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(<1..736)
                     /locus_tag="PW004_002861"
     CDS             complement(<1..736)
                     /locus_tag="PW004_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2727773.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSI"
     gene            complement(747..1415)
                     /locus_tag="PW004_002862"
     CDS             complement(747..1415)
                     /locus_tag="PW004_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDL2727774.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
BASE COUNT          539 a          241 c          186 g          498 t
ORIGIN      
        1 caatcgaact attaaaaatg tcaaatttcc ttaatgcgtt attgttttca gtttttaaca
       61 actgaatagt tgtagttttt acagttctat ttttaacaaa tagaacttga tcaattaatt
      121 ctttcaaata gtaaaaacga tgttcagcta taacaatagt aaatccttta ttttttaaat
      181 cacaaagtaa gtcagcaagt ttcttagtcg ctttgaagtc caaatttgaa gacggttcat
      241 ccaatataat taatttagag tttagcgata aactacatcc aatagaaatt ttctgccgtt
      301 caccacttga tagcgaaaaa atgtttttat ctaatatatt tgaaagatct aataattcag
      361 ctaattcatt gattctttta tccatatgct cttttgtaaa tccaaagttt tccatcgaaa
      421 atactaactc actcgttgta tttgttgtaa agaattgtcc tctaggatct tgacttacgt
      481 agccaatatt ttttgatata ctttcttgtg ttaaggattg tagcgagtta ttttcaaata
      541 aaatttcccc aaccaatttt ccgtcataaa gtcttggaat aagtccattc attaacttta
      601 atagtgtgga tttcccactc ccagaagaac cagtaatcaa tgtaagacta ccttttttta
      661 ttgaaaaatt aaggtcaaca agcgcttgtt ccttaacatc cttatatgaa aaagaaacat
      721 tgttaaattc aaacaattct ttcacctcaa atcaaaatac ttaagaaaaa aatagtaacg
      781 gaaaacagga tgatatagtc taataagctc atgtttgttt tatagtaaga acttcttttc
      841 acaggagatt ctatcccctt tgttattgca ttagcggtca tttcttcagc aatcatagtt
      901 gcacgtataa gaacgggaac taacagaaat tccaaatatt gcagaggctg aaaaaaagac
      961 aagcgaatgt ttctcatttt taaagattct ttaatcattc ttatttcaga acgataagtc
     1021 ggaaagaatc tgatcactac tgtaatactt aacattattt ttttcggtaa atgtattttt
     1081 tctaaagagg ataataattc attgctagta tagacactaa ccaacaacca tgagatcatt
     1141 acgaccggca acattcgaaa tacaatatat aaggatgtcc caaaaaatat agtaaaaaac
     1201 tctatcttga tagcatcaat aattttcatt actaaaaaca gaactgaata aatcaagatg
     1261 aaataaatac atgtctttat tttacgaaac atcaatacta aaactgtagc aataatgaaa
     1321 caccccactt gaaatatatt tccctttcct aaaaaaataa tgaccggaaa aaaaatattt
     1381 aaagtaatca aggttaacgg attgattttt gacactcttt cacctccaaa aaataagatc
     1441 gcactgttat cttaagctga taaa
//
LOCUS       DANKKG010000267         1455 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000267 DANKKG010000000
VERSION     DANKKG010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.273"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            75..1415
                     /locus_tag="PW004_002863"
     CDS             75..1415
                     /locus_tag="PW004_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDL2727775.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
BASE COUNT          462 a          321 c          284 g          388 t
ORIGIN      
        1 taataaacgc aaacagacac cttgaaatcc tttagaataa agtcgacgaa aaccattcaa
       61 aggagtgttt caagatgtcc tattcagagt ttaccaaaga gttactagat attctagacc
      121 taaatcttac ctttcatgaa gatgcctttc gcaaagagcg cattaatgac gaaacatgtt
      181 ttgtttttga tggcacattg acctatcaac cagaggaatg cttccattgc cactatcaaa
      241 ataagcaaac gatcattaaa tggggctgga aaaaagtatc catcttattg aatgatgtca
      301 gtaactacaa gaccattgtg cggattaaca aacagcgctt caaatgtaaa cactgtggaa
      361 agactttttt agcagaagat tcagttagcg atcgtcgctg ctccattgcc cgcagagtca
      421 aacaagccat tcttgagcta ttaagtgagc ctatttctat gtctttgatt gcacgtatga
      481 agcacatttc tccgaccact gtcattcgaa tacttcgcag ccttcggcct aaaaccgttt
      541 ctttgaacca gccgttacca gaagtggtgt gtttcgatga attcaaatcg gtgaaaaacg
      601 tctcgggagc catgagcttt gtcatgatgg acggcaagac ccatcaactg attgatattg
      661 tggaaaatcg gcaactcaat cctttacgcg actatttctt gcgctaccct agaaaagtcc
      721 gtgaacaagt acgtctagtg gtttcagatt tctatacacc ctatcgcaca ttggtgaaag
      781 agtgttttcc gaatgcccga atcgtcgccg atcgctttca tattagccaa cacatcggtc
      841 gggcctttac caaccaccga attcaagtaa tgaagacctt taaaaaaggg gatcgtcggt
      901 cgaagcactt aaagaaatac tggagactac ttcaaaagaa tgcgtgggaa ttgaatggtc
      961 agcatcgcta ctggcgtccg tcatttcgag atcatctgac agaagcggag atagtcgatc
     1021 ggctattata ttatgatgac tccttaaagc gaggctacga agtctaccag gtttttcttt
     1081 ccgctatcag aagacaagat gttcctgaat tcgtcgcgct cctaaaagaa gactacaagg
     1141 agttgccaga gcactatcaa ccagtgttca cgaccttcaa gaaatatcga acagaaatca
     1201 aacgagcgtt gcgagttccc tattccaacg gccctataga atgtttgaac aaccatatca
     1261 aagtactaaa acgaattgcc tatggcttta gaaactttca aaactaccga gaacgaatct
     1321 ttttatatcg aggaaaatat ttcaagaaaa ctaagaacac tacccaactg actaaagcca
     1381 gaacaacaac acggctagac aagatagctg tctagccgtg tgagtatctc gattattcta
     1441 ttgaatttct tcacc
//
LOCUS       DANKKG010000268         1453 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000268 DANKKG010000000
VERSION     DANKKG010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.274"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(<1..648)
                     /locus_tag="PW004_002864"
     CDS             complement(<1..648)
                     /locus_tag="PW004_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL2727776.1"
                     /translation="MKGDVIVEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAG
                     ANKKLVYNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLK
                     ILTTFLYVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKF
                     VYDDFSDELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMI"
     gene            973..1389
                     /locus_tag="PW004_002865"
     CDS             973..1389
                     /locus_tag="PW004_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDL2727777.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            1386..>1453
                     /locus_tag="PW004_002866"
     CDS             1386..>1453
                     /locus_tag="PW004_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2727778.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKS"
BASE COUNT          481 a          227 c          269 g          476 t
ORIGIN      
        1 aatcatatgg aagccaccga ttaggtattt tatgggttta gtcggaaatg tttcaagagc
       61 aatttccatc atatcgataa tgccatgatg actacagcca gtaaagacaa tcagaccgtc
      121 ttcttgctca attacgacca ataattcatc tgaaaaatca tcataaacaa atttatcatc
      181 catctttttg tataaattct tggtggtcga gtttgctttg cctaggggga tgatatggaa
      241 gctcggagca agttctgttt tcgtttttat tgtatgtaaa ttctcatgca ttttaaggat
      301 ttcattttta ggaataccaa catacagaaa cgtggttaaa atctttaaat aatggggtaa
      361 agttacacct tcagctgtat aaataggggc gtttcctgat tttaaatatc ttaacccgcc
      421 ggtgtggtca gaatgaccgt gtgaaataat cactgcctcc agatcattca aagtttcccc
      481 cattttagtc gcattataga ctaatttttt attagcgcca gcatcgaaga gatatttctt
      541 ccctgagtag ttaattaaga gagataaccc atgtccactt ttaaagttca aatttattct
      601 tgagttttca aataaaacct tgatttccac tataacatct cctttcacat gaacgtgtat
      661 tcatatattg tagcacatta agttctattt tttgttgtaa ttaccgaaaa aaatattaat
      721 ggacgctttt tacgctttat ttcgtatgtg gaagaaaatt tcttaggatg tggcggggga
      781 ttggggcaaa gctaaattcc aattacagtg atcatttaat tagacacctt taaataccca
      841 cactttcatc aacactataa tttagggaaa gaaaagtgtt ggcatgatat agaaaaacct
      901 ttaattaaca gcgtttccta aaattggcac gcatcttgct tatagaaaag cgtaaggata
      961 ggaggaatga aaatggcaac tataattctt gccagccatg gggaatttgc tcaaggatta
     1021 aagcaaacag ctacaatgat tattggagat agtgtaccaa ttcatgcctt gtcagcattt
     1081 agagatgaag atgaatctat tttggtacaa ataaaaaaac ttttagccac aatagatata
     1141 gaagaaacct acattttaac tgatattcta ggtggtagtg tgaataatga tatgctcact
     1201 attattaaag agtatgaggg attgcaatta attactggaa tgaacttacc tttagttatt
     1261 tctattgcaa cctataatgg aaaaattggg gaaagtgagc tagaaaagct tattaaagag
     1321 agtcaacaat cagtgattga ttgtagaaaa ttattagtag aacaacttac tggaggagat
     1381 gacctatgat taagttagta cgtattgacc atcgtttgtt gcacggacag gtagtatttt
     1441 cttggagtaa atc
//
LOCUS       DANKKG010000269         1448 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000269 DANKKG010000000
VERSION     DANKKG010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1448
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.275"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            837..1049
                     /locus_tag="PW004_002867"
     CDS             837..1049
                     /locus_tag="PW004_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2727779.1"
                     /translation="MKNRLEELRKQRGIKQEDLASALEVSRQTIGSLENGRYNPSIIL
                     AFKIARYFQMSIEEIFIYEEESEDEK"
     gene            1039..1434
                     /locus_tag="PW004_002868"
     CDS             1039..1434
                     /locus_tag="PW004_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727780.1"
                     /translation="MKNKGQIWYIGYIVSLFLIVTILVTDFPKMVDIGLAILFSAVFG
                     VSHVQILHDKMIKNDADYKINVMDERNIIIKEKAGNITNMFNTILLGVATVIFICLDY
                     IIPAIIVGSIIAIQPIILIIISSVLEKKM"
BASE COUNT          501 a          215 c          244 g          488 t
ORIGIN      
        1 agtactctag tatttgtgtt acccgaataa ttacttgatt atattggttt cccatataat
       61 tgaaggctgt cctaattttt acctactata tgatcataat tgattaactc ttatatattt
      121 ccgaaaatta tcttgaagat ttctattggt atggagaata tccatttcat tgccaataag
      181 gatataaatt ctcaaaataa caatcgatgt ctgtattaaa caagcctaga atggtaaatc
      241 tgattcgcca aaaccattgg attgtatcga tttgctttac gcgaacgcca cctacccatt
      301 ttataaaaat aaaatgggca aaagatcttt ctttttgtca tacaaaaaga agaaaaaaaa
      361 gagacgattt tcttcttcca aaatcgtccc tcttttttct aaaaccatgg atgaacgaac
      421 aaaaaatctc tcgtattttg tttgttcatc cactaaaaat atgcgaacag tatggtttca
      481 ttcaatcctg ttcttacaag tcaaaagcat cgaatcgtcc ttggacgatt caacaaaaaa
      541 agtagtcgtt tttggatgac tactaagttc taaacattca ttacccaaaa tcaatttggt
      601 ctaacatcgt ttctcgactg gcttgatcta atcggctctt gacagggctt tttcttttct
      661 gttgatagca gataagagga aataagaata agtgtgttca tgtgtcataa aaacagtaac
      721 taaaacatta aaatcaaaat ttatatggaa agcttccttg acattttgtg ctcgcaggag
      781 tattataaaa gcacgaaagg aaagtaaact ttccattttt tagaaaggag catgttgtga
      841 aaaatcgttt ggaagaatta cgaaaacaaa gaggaattaa acaagaagat ttagcttctg
      901 ctcttgaagt atctcgtcaa acaataggtt ctttagaaaa tggacgttat aatccctcta
      961 ttatattggc atttaaaatt gcaaggtact ttcaaatgag cattgaagaa atttttatct
     1021 atgaggagga aagtgaagat gaaaaataag ggtcagattt ggtatatcgg ttatattgtt
     1081 tcgttgtttt tgattgtgac aattttggtt acagattttc ctaaaatggt agatattgga
     1141 cttgctattt tattcagtgc cgtttttggc gtttctcatg ttcaaatttt gcatgataaa
     1201 atgattaaaa atgatgcaga ctacaaaatc aatgttatgg acgaacggaa tatcataatc
     1261 aaagaaaaag cgggaaatat aacaaatatg tttaacacaa tactgttagg ggtggcaaca
     1321 gttatattta tttgtttaga ctacatcatt ccagctatta ttgtcggaag tatcattgca
     1381 atccagccga ttattttaat cattatcagt agtgtgcttg agaaaaaaat gtagaaataa
     1441 aggttcat
//
LOCUS       DANKKG010000270         1442 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000270 DANKKG010000000
VERSION     DANKKG010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1442
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.276"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..780
                     /locus_tag="PW004_002869"
     CDS             <1..780
                     /locus_tag="PW004_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL2727781.1"
                     /translation="LTKSEKKVADFILNSGKTIIYSTMSDIKKNTKVGDATIIRFCQK
                     IGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYSTIRLLDEEKLNEAI
                     KMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQAQVASLLTVNDLVI
                     IFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQTAIEEFLNGGSLA
                     GKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
BASE COUNT          498 a          209 c          230 g          505 t
ORIGIN      
        1 ttaacgaagt ctgaaaaaaa agtcgctgat tttattttaa attctgggaa aactattatt
       61 tatagtacga tgagtgatat taagaaaaac acaaaagttg gcgatgctac tattattcgc
      121 ttttgtcaaa aaattggttt ttctggtttt tctgatttaa aaattgaaat tgcaaaggaa
      181 gactttagtc aaaaaaaaga acatccgact agtgtaaagt actatgatga aattgctaat
      241 agtttaacag aagctctcta ttccactatt aggcttttag acgaagaaaa attgaatgaa
      301 gccattaaaa tgatcactca atcaaaaaac atatatatat ttggagtggg atctagtgga
      361 aatactagtc ttgatttaga aaatatgttt cttcgcgtag gagttcaagc aaaagctgta
      421 ctagaccctc attttcaagc ccaagtcgct tctttattaa cagttaatga tttagtaatc
      481 attttttctt tatctgggaa aacgaaagat acttatgatt cattgaaaat agctaaaaaa
      541 aatggcgcaa aaattattgc tataactaac tatattcatt cacctatagg aaagtctgca
      601 gacttagttt tacaaacagc cattgaagaa tttcttaatg gtggatcttt agctggaaaa
      661 atatcacaac tttatatttg tgatctatta gttcatggtt atgaacaaaa caataatatt
      721 gattcagttg aattaagaga aaaagttttg cgctctatta ttgataaaag tattgagtga
      781 actaccccca cttagctaaa cctaacggtt tttaacgatt gaagtaggca attcttggga
      841 tgagagcata cttgatagct tattttttac taatagcggt ggctcttatt gaccaaatat
      901 ccctgtagtt cctacggttc ttgattttat ctaaagatat ttttatcgtg gaagctattg
      961 atcaacttga aagaaaaacg tgtagctcat tatcactagt ttatcgagta tggatgaagc
     1021 aatggatcat cctctcttaa ataaatttat taaggatctg atccttcaaa ttttttccat
     1081 gatcgatgag caggaaccta ctgaatctaa acgacatcgg gcacaaggaa ttattaagat
     1141 tgctaagcga atgttttcta taaagggtgt tctaagctat acagtgttaa ttcaaaagat
     1201 tcccaatcat cttgtttata agaacaacgt tgaagattaa aaaagtgttt caatgctaaa
     1261 attgcaaaat acttcaatgt tatgagacaa actgtctata agaatcaatt tccacccatt
     1321 tcatcacttg ttaaaagttt atgatccttt tgatttttat attaatttat ctgtatttaa
     1381 tacagatttt atttattagt tcatctgcct attgtatttt attttgttgc cttaccatct
     1441 ga
//
LOCUS       DANKKG010000271         1437 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000271 DANKKG010000000
VERSION     DANKKG010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.277"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            337..1422
                     /gene="xerS"
                     /locus_tag="PW004_002870"
     CDS             337..1422
                     /gene="xerS"
                     /locus_tag="PW004_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HDL2727782.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
BASE COUNT          552 a          244 c          200 g          441 t
ORIGIN      
        1 tctttaactt ctccctcccc ttttttattt gtatatacat tcatatacgc ttgttaatat
       61 caaatcaacc gtagtctctc ttatgtctaa ttatatagaa agattgttct ttctatataa
      121 tttagacaat ttaaaaaatt aagattgcct ccttctgttt cctttcctat tcatgtatac
      181 catttatttt aaattattaa atctatacct atttatctgc ttttttgaaa taaattaata
      241 aatttcagag tataccaatt gacaaaacac tctataattg gtatagtcca attatagaga
      301 tactcctctc ccctacttat cgaaaggatg aataacatga aaaatacaga aatatatcaa
      361 aaaatagata caatcatact tcatatgccc gattatataa aagattttgt tcaagatcgt
      421 gaggataaag atcaatcacc tcgaactact ttagaatatc taaaaaatta taagctcttt
      481 tatgaatggc ttctgagtga gtctatcgtt cccgatactt caatcacctc gataagacag
      541 ttaactgcct atgatctaaa cttgtacaaa tctcatttga aacgtcgcac aaaggaaaat
      601 gtaaaaaaag agacaacaaa aactaagctc aaagataata ataacttagg tctatctact
      661 tctactatca atagaaatat cacagcatta aaggttctat ttaaatatct ttcgaaaagt
      721 tccaataatc ctttaggaaa accatactta gaaaataatc ctatggatca agtagcaact
      781 atcacagata aaactacgtt agctgcccgt gcaaacgcaa tcgagaaaaa attatttcta
      841 gacgaagata cacaaaatta tctagattat attgccaatg aatataaaaa aactttgagc
      901 aaacgagctt tgatctatta tcaacgagat gtagaaagag acctggcgat caatgcattg
      961 attttaggat ctggattaag actctccgag gttgtaaata ttaatttaga ggacctatct
     1021 ttagaaaaga ataatgtggt agtaactaga aaaggaaata agagagacgc agtaaatata
     1081 gcagcatttg caatggaata tttagctaat tatctagcta ttcggaaaga acgatacaaa
     1141 gtaacagaaa acgaaaaagc tttatttcta gcaatttatc aaggagaagc caagcgtata
     1201 tccggtattg caatcgaacg gatggtagca aaatattcaa aaggattcag agtccaagtc
     1261 agtccacata aattacgtca tactttggct actagactct atcaacagac aaattcattg
     1321 gtattaactg cccaacaatt gggacataca agtacaaata caacaacttt atatactcat
     1381 atagacaatg cagctacaat tgatgctttg aacgctttgt aaatgtagta aaaaata
//
LOCUS       DANKKG010000272         1436 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000272 DANKKG010000000
VERSION     DANKKG010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.278"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            175..789
                     /locus_tag="PW004_002871"
     CDS             175..789
                     /locus_tag="PW004_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2727783.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            complement(813..1163)
                     /locus_tag="PW004_002872"
     CDS             complement(813..1163)
                     /locus_tag="PW004_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDL2727784.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(1157..1420)
                     /locus_tag="PW004_002873"
     CDS             complement(1157..1420)
                     /locus_tag="PW004_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HDL2727785.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
BASE COUNT          457 a          271 c          231 g          477 t
ORIGIN      
        1 tacaaaaaat attgccattt aaaaagaaaa gtgacacata ctatttcctc catcattagt
       61 ttttgtctat ataaggtata ccctaactag acacgaccgc gacattgaaa aatctggttt
      121 tctcgtgtat gattagggtg ttttttaaat agttgcagaa agtaggtaaa aattttgaga
      181 gttgcataca tacgtgtctc atccatcgat caaaatgaac aacggcagat tgaagaaatg
      241 aaaaaattcg gtgcagagag aattttcatt gagaaacaat ctggcgcaac gattactcat
      301 cgacctgttt ttcaagaagc actggatttc gtccgagatc aagatatttt tattgtggaa
      361 gctattgatc gacttggaag aaattacgac gagatcatcg cttcagtcaa ttacttgaag
      421 aagaaaaatg tgcagctgat catcacaagt ctaccgatca tggccgaagc aattggcaat
      481 cctctcctgg ataaattcat taaagatttg atcattcaaa ttttggccat gatcgctgaa
      541 caagaacgta ccgaatctaa acgacgtcag gcacaaggca ttaagattgc aaaagcgaac
      601 ggtgtttata aaggacgtcc taagctatac agtgccaatg caaaagatcc ccaacgtcgt
      661 cttgtttata aaaacatcgt tgaggatcta aaaaaaggtg ttgcgatcgc taagattgca
      721 aaagactaca atgttacgag acaaaccgta tatagaataa aaaaagacag catggtcaat
      781 cacgaatagt tgatcatgct gctgtttcta ttttatttgc ttactttttg aaaaactgtt
      841 ctaactttca tcaataactc ctcaactaaa ctatcatgcg ctttttccaa aaaaatacac
      901 tctctggctt cataatcaat tgtcgttagt tgatctaata atacttttcc tgttgtcttc
      961 atttcatatt ctgagtttat atttacatat agcggatagt ttctagttgt actagaaatt
     1021 ggagcaactg taacaacgtt actataatcc gcaataatat catgacttaa aacaatataa
     1081 ggtcttttac ctgtctgttc gtgtccagat cgaggtgctg tattcaataa tagaatatcg
     1141 ccttgtctag gttttctcac catttttcat tacctactgc ttcacctaaa ttttgaatcg
     1201 tcacatgatt tctttcatca gtgtaatttt caaataactg ttcaattgtt aattcatttt
     1261 ctatattagc cgcttcgata attagccgct tctgaccgtt tacttcttct tgtattatct
     1321 tcactttgtc ttctgtatga attccaagat attccataat atactttggc aatctaaggc
     1381 cattactatt tccccatttt tttataggta acatttccat agtagacacc tctttc
//
LOCUS       DANKKG010000273         1424 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000273 DANKKG010000000
VERSION     DANKKG010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.279"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(150..1337)
                     /locus_tag="PW004_002874"
     CDS             complement(150..1337)
                     /locus_tag="PW004_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HDL2727786.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          414 a          309 c          249 g          452 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact ttta
//
LOCUS       DANKKG010000274         1420 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000274 DANKKG010000000
VERSION     DANKKG010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.280"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..252
                     /locus_tag="PW004_002875"
     CDS             <1..252
                     /locus_tag="PW004_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727787.1"
                     /translation="FNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDKTKLDGIAA
                     GAQKNPGNATTTTAGLMSATDKAKLDGLANITFEKVGTV"
     gene            252..746
                     /locus_tag="PW004_002876"
     CDS             252..746
                     /locus_tag="PW004_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727788.1"
                     /translation="MADIVQLKEDGVAKYLKTHVEAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     DSVILTVPEGYRTPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVI
                     TYSI"
     gene            760..1191
                     /locus_tag="PW004_002877"
     CDS             760..1191
                     /locus_tag="PW004_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727789.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            1193..1330
                     /locus_tag="PW004_002878"
     CDS             1193..1330
                     /locus_tag="PW004_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HDL2727790.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITEEEADKIISKEESA
                     S"
BASE COUNT          519 a          209 c          312 g          380 t
ORIGIN      
        1 tttaattttg ttttaccaaa gggagataaa ggtgatcctg gaataaatgc cacaactaca
       61 gctgtagcta caactacagc aaatgggttg atgtcgtcca cagataaaac taagttggat
      121 ggaattgcag ctggagcaca gaaaaatcca ggtaatgcta caactacgac agcaggttta
      181 atgtcagcaa ctgacaaagc taaactcgat ggattagcca atattacatt tgagaaagtg
      241 gggacggttt aatggctgat attgtgcaat taaaagaaga cggagttgct aaatacttaa
      301 aaacgcatgt agaagctatt gatggtaaag aagcattggt gcagacagat ggagaccaag
      361 ccattgcagg acataagaat ttttcaggtt ctgtaacaat caataataaa cgtgttttga
      421 cgactgatga taacagatat gaagtggtaa atctggttgt cactaacggt aatacaggga
      481 cagcaaagct ttatcgtgaa ggaaaaacag tttctatata ttttgtggct ttaaacggaa
      541 aaagtagtgg cgggaatgat tcggttattt taactgttcc agaaggctat cggacaccaa
      601 ttagttttga acaactggtt gggtcaatag accgttctac tttgaacagt gctcaactat
      661 ctattggcgc agacggagcc attaaatgga gaagaaattc aagttatgga tcagcttatt
      721 catttgttat cacttattca atttaaggga ggaaatctaa tgaaagtagt ttacaaatca
      781 atcaagcctt acggattcga gcaaatcatt ttgaacaatc aagaaaatat ccctgaaaac
      841 tgtacagaga ttaaaccacc agttcctaac tggagaccaa gatttgattt tgataaaaaa
      901 cagtgggttg agttagctac tgaagaagag aaaaaaggga atgctgttga tagcgtagat
      961 gaattggcta atattaaggc attatacgaa acgctgaagg cagaaaatga tgaattgaaa
     1021 cagctaaatt ctaaagcaat gctaaacaat gtagcaatca agcaagaaaa tgtcttattg
     1081 aaagaaaagt cagaaagttt agcgcagttg aattcaaaaa cgatgcttgc ttctgtacaa
     1141 aataccaagg aaatcgaaga aatcaaaaaa caattacaag gtgggaagta acatgtattc
     1201 atatgaagat atcaaactaa tgtatgactg gggctgtttc actaacgaac aagtaatggt
     1261 tttcgttccg ttgtgcatta ctgaagaaga agcagataaa atcattagca aagaagagag
     1321 cgcatcttaa ttgatgtgct ttttattttg attcaaggag ttgtcacatg attaatttag
     1381 gggaatgggg agcgatagca ggatcaataa ccgctatcgt
//
LOCUS       DANKKG010000275         1402 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000275 DANKKG010000000
VERSION     DANKKG010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.281"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..196
                     /locus_tag="PW004_002879"
     CDS             <1..196
                     /locus_tag="PW004_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HDL2727791.1"
                     /translation="FVYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPVGYRNQ
                     RLAQRILDNELGCANASEAV"
     gene            331..552
                     /locus_tag="PW004_002880"
                     /pseudo
     CDS             331..552
                     /locus_tag="PW004_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208413.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
     gene            552..899
                     /locus_tag="PW004_002881"
     CDS             552..899
                     /locus_tag="PW004_002881"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727792.1"
                     /translation="MRKRTIKTQLAVSFLAIATLIIGSISLVALSLMNNHFSKYVEER
                     QEDLLNQYVYTIDLLWLNSGETWNSEELAALSEKVLENNIYFSIEDEQGNMVWELTGK
                     DLKSAQEKLKKMH"
     gene            896..>1402
                     /locus_tag="PW004_002882"
     CDS             896..>1402
                     /locus_tag="PW004_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HDL2727793.1"
                     /translation="MKVSEKNSVKLDETIEVKKKLINDGNEFGKVTFYYFGPFAYTEH
                     DALFISSMKQSLMYVAIAALLVSFILASWISARLGLPLKHVSDFTHKLTRGEYADKIP
                     QETSIIEINSLIDSLNDLSNQLEKQHGLRKRLTTDISHELRTPLATLKGNVEGMIDGV
                     WKITPERLQ"
BASE COUNT          489 a          224 c          283 g          406 t
ORIGIN      
        1 atttgtctat caatacacat ttgaaacctt acaacaattg gatttggagt tatttgacta
       61 tgtcaattgg tggaaccacc ttcggttgca cggtacactt ggctacgaga caccggttgg
      121 ttaccgtaac cagagattgg cgcagcgaat ccttgataat gagctcggat gtgctaacgc
      181 tagcgaggca gtctaacttt aactgttagc tcctgccgaa gatcgtcaca tccgaggagg
      241 ctcattgtca aggacaatcg gaatagcata cggaaagaag ttctgcacct tataaaattt
      301 gtcaaaaaaa ctgttgccat tccacttaaa acaactgaat ttgatattct acacgctcta
      361 atgtccacac ctaataaagt cttttctcgt agtgatttga tagagaaagt taagggaata
      421 gaatttgatg gactagatag gagcatcgat tctcatataa aaaacttaag acataaaatt
      481 gagaatgatg ctaaatcacc tatgtatatt ttgacagtcc atggtacagg atacagattt
      541 ggtggccact aatgagaaaa agaacgataa aaactcaatt agctgtttcc tttttagcaa
      601 tcgcaacatt aataatagga tcgattagtt tagtcgcatt aagtttgatg aacaatcatt
      661 ttagtaaata cgtggaagag cggcaagaag atttgttgaa tcagtatgta tatacaattg
      721 atttattgtg gttaaatagt ggggaaacat ggaatagtga agaacttgca gcactatctg
      781 agaaagtatt agagaataat atttattttt ctatagaaga cgaacaaggc aacatggttt
      841 gggaacttac tggaaaagat ttgaagtcag cacaggagaa actgaaaaaa atgcattgaa
      901 agtttctgag aaaaacagtg tgaaactaga cgaaacaata gaagtgaaaa agaagttaat
      961 taatgatgga aacgagtttg gaaaagtaac gttttattat ttcggacctt ttgcttatac
     1021 agagcacgat gccttgttta tctccagtat gaaacaaagt ttaatgtatg ttgcaattgc
     1081 agctcttctt gtatcattca tacttgcttc gtggatctct gctagattag gattgccact
     1141 aaagcatgta agcgatttta cacataaatt aactcgtggg gaatatgctg ataaaatccc
     1201 acaagaaact tcaattattg aaataaattc cttaattgat tccctaaatg atttaagtaa
     1261 tcagttggag aaacagcatg ggcttcgaaa aagattaact accgatattt cacatgaatt
     1321 aagaactcca ttagctacgc ttaaaggcaa tgtggaagga atgattgacg gagtctggaa
     1381 aataactccc gagcgcctac aa
//
LOCUS       DANKKG010000276         1393 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000276 DANKKG010000000
VERSION     DANKKG010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.282"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            128..1333
                     /locus_tag="PW004_002883"
     CDS             128..1333
                     /locus_tag="PW004_002883"
                     /inference="COORDINATES: protein motif:HMM:NF033550.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDL2727794.1"
                     /translation="MSMNHFIKQLFTIKDSNIQIQSFEETFYKSRKSIVIEGTLIRTY
                     RRCPCCKDSTKQIVKNGKKISMILLNRSGNKRTYLRLKKQRYLCRACKKYFTARTYLV
                     TPFCFISKQIHYKILEELTERQSIKAIGKHCDVSVTTVQRTLSALSSDIRQNLEYLPR
                     CLLFDEFRSLSTWHGKFSFSCMDGETGKLFEILPSRRKKDLVAFFMRFSLKARLGVHY
                     IVTDMNAPYFSLVKECFPNAQVIVDRFHIVQHLNRFFDAVRKRVMKTLDQKDPIQAKQ
                     YRQLKSLFKLLLKSEDRLDYNTLTKRRNFNWRLLTETEVVDRLLKISPELKTAYRYYQ
                     DLLTAYQEKDPDTFFQLLRAMPGEVPLELHHIKKAFFKYEKGIRLALLKKYSNARLEN
                     LHTGSVAKF"
BASE COUNT          445 a          271 c          254 g          423 t
ORIGIN      
        1 ctctataatt tctaggactg atggaaattt tctatctgtc ctagtttttt tgtaaaaaaa
       61 caaaaagaca tacaaaattt ccggtagaat agagtctcct acaacatcat tctgggggaa
      121 ttcttgtatg tccatgaatc attttatcaa acaactcttt actattaaag actcaaatat
      181 ccaaattcaa tcctttgaag aaacttttta taaaagcaga aaaagtatcg ttattgaggg
      241 aactctgatc cgtacctaca gacgttgtcc gtgttgtaaa gattctacaa agcaaatcgt
      301 taaaaatggc aagaagatct ctatgattct cctgaatcgt agcggtaata aacggaccta
      361 tcttcggttg aagaaacagc gatacctctg ccgagcatgt aaaaaatatt ttaccgctcg
      421 gacgtatctc gttaccccat tctgctttat ttctaagcag attcattaca agatcttaga
      481 agaattgaca gagcgtcaat ctataaaagc aattggtaaa cattgcgatg tttctgtgac
      541 aactgttcag cggacattgt ccgccttgtc atcagatatc cgtcagaatc ttgagtattt
      601 acctcggtgt ctattattcg atgagtttcg ttctctttct acatggcacg gtaaattttc
      661 tttttcttgt atggacggtg agaccgggaa actgtttgag atcttaccta gccgccgtaa
      721 aaaagatctc gttgcttttt tcatgcgttt ttctctcaaa gcgaggttgg gtgtacatta
      781 catcgtaacg gatatgaacg ctccttattt ctcactagtt aaagagtgtt ttccaaatgc
      841 acaagtgatc gtcgatcggt ttcacattgt tcaacatctg aaccgcttct tcgatgcggt
      901 tcgaaaacgt gtgatgaaaa cacttgatca aaaagaccca atacaagcta aacagtatcg
      961 ccaattgaaa tccttattca aattactttt aaaatcagag gatcgtttag attacaatac
     1021 actaacgaaa agacgcaatt tcaattggag actattgact gaaacagaag tagttgaccg
     1081 ccttctaaag atttctcctg aacttaaaac agcttatcgt tactaccaag acctgcttac
     1141 tgcgtatcag gaaaaagatc cagatacttt tttccagctg cttcgagcaa tgcctggaga
     1201 agttccattg gaactgcacc atatcaaaaa agcctttttc aaatacgaga aagggattcg
     1261 gctggcatta ctgaagaagt attcgaatgc acgactggaa aatctccata cgggttctgt
     1321 tgcaaagttt taaatctact atcaaataag gtagaataat agaaaaagat agcaggagga
     1381 atgacgatga atc
//
LOCUS       DANKKG010000277         1372 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000277 DANKKG010000000
VERSION     DANKKG010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.283"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            113..1345
                     /locus_tag="PW004_002884"
     CDS             113..1345
                     /locus_tag="PW004_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDL2727795.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
BASE COUNT          449 a          260 c          301 g          362 t
ORIGIN      
        1 aaccttaaaa aaataacctc ctttttttat ttaaagcctc attgtttgaa tcataattaa
       61 atggtatcat gctaatagtt accagaaaaa atggtgagga ggaattttta ttatgttatc
      121 tgattttaat gaacttctaa aaaaatatgc acgcttaatt gcagaaactg gcgtagccac
      181 agaaaaagga cacacagttg ttttacagat cagcgtggaa caggcacccc ttgctcgatt
      241 gatcacacaa gaagcttata aactaggagc cgctgaggtt atcgttcaat ggactgacga
      301 ccagatccaa agagaatttc ttttacatgc tgcaacggat cgtattgaga atgtaccaca
      361 atctaaaatc gatcaagcgg atgaatggct tgaaaaagga gcaagcagga tcagcgttgt
      421 ttctgccgat cccgatgcat ttgctggtgt agatagccat cgagtagcgt cttatcaatc
      481 agcgaatggc aaagcactaa tgaatttacg caaggcaact caagcaaata aagtcagttg
      541 gactgtcgtg gctgcggctg gtaaacaatg ggcagcaaaa gtcttcccag aactgccaga
      601 agaaaaacaa gttgaggcat tatgggatca gattttcaaa acgactcgtg tctatgaaga
      661 agaccctgtc ttagcatgga agaagcatga tgaaaaatta gcaaaaaaag cagaagaatt
      721 gaatcgagaa caattttctg cccttcacta tactgctccc ggtacagata tcattatcgg
      781 attgcctaaa aaccatcttt gggaaggcgc tggaagctat aatgctcgtg gagaaaaatt
      841 catggcaaat atgccgacgg aagaagtgtt cacagcacct gacagtcatc gagtagatgg
      901 ttacatttca agtacaaagc cactgagtta tgctggcacc atcatctcgg gtatgaaatt
      961 tacttttaaa gatggaaaag ttgttgattt ttctgccgaa caaggagaag atgtcttagc
     1021 aaaactgctt gatacagatg aaggtgcgcg tcgtttagga gaagtcgcac ttgtacctga
     1081 cccttcacct atttctcaat ctggcatcat ctttttcaat acattatttg atgaaaacgc
     1141 atcgaatcac ttagcactag gttctgctta tgccttcagt gtgaaaggcg gtacagaaat
     1201 gtccgatgaa gaattagcag aagctggatt gaatagaagt caaacccacg tagatttcat
     1261 ggtaggatct gacaagatgg atatcgacgg aatccgtgaa gatggctcta cagtaccgat
     1321 tttccgaaat ggcgattggg cttgagtata ctttcgcaca catgcaaaaa at
//
LOCUS       DANKKG010000278         1367 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000278 DANKKG010000000
VERSION     DANKKG010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1367
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.284"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            56..1351
                     /locus_tag="PW004_002885"
     CDS             56..1351
                     /locus_tag="PW004_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDL2727796.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
BASE COUNT          432 a          272 c          262 g          401 t
ORIGIN      
        1 acaccctgaa atcctttaga attaatttgg ctaccaattc ataggaggat tcaagatgtc
       61 tcatactcag cttatcaaag ataccctaaa tattctagac ctaaatatct atttcgagga
      121 aaattgttta actaaagaaa aatacaaagg gcaaatttgc ttgatttaca aaggaactct
      181 tctgtacaag ccagaagagt gttcccattg tctttgtgtc gtcccctcta ggatcattcg
      241 ttgggggacg acaaccgttc ggttgttact gaatgatgtg tctgagtatc gcacttatct
      301 ggagctgaag aaacaacgat ttaaatgtaa gtcttgccaa agaacattcg tagcggatac
      361 ctctgttgct gaaaaacact gtttcattag tcaaaaagtt cgttggtctg tcattgctcg
      421 cttaaaagag aatacttcca tgacggaaat cgctcgtcag aaaaatattt ccacttcttc
      481 ggtctatcgt gtcatgaagc gattctaccg cccattgaat cctttcaaac agactttgcc
      541 aaaggtctta tgctttgatg aattcaaatc ggttcgaagt gttgccagtg caatgagttt
      601 tatcatgatg gacggacaaa gccatgcatt attggatatt gtcgaaaatc gacgcttgcc
      661 ttatttagaa cgctattttt ctcggttttc attagcgact cgtgaagccg ttcaattgat
      721 tgttattgat atgtatgcgc cttatgtttc gttggtgaaa aagcttttcc cgaatgctca
      781 gttgatcatc gatcgtttcc acatcgtcca acatattggc agaactttct tgaatcatcg
      841 agtgaaagaa accacggtac gacttaaagg aaattcccac tctcaacgag ggttagggaa
      901 aaaattgaag cgctattgga aactcctaca aaaagaagaa gcaaagttaa actatgaaaa
      961 acgggtttgg cgtgccagtt ttaaagatta tttgactgaa agtgagattg ccgatcgact
     1021 gttagctgct tgtcccaact tacgccaagg ctatcagtta taccaagaca ttctttatgc
     1081 ggtaaagaaa agagatcagt cactatttga ggattgttta actagagaca ttagcggtct
     1141 gccagaaacc tatacaacca ctttacgaac gtttaaaaag tttctgccac agatccaaaa
     1201 tgcgcttcac tacagctatt caaacggacc attggaatgc ttaaacaacc acatcaaagt
     1261 attaaaacgc aatgcgtacg gctttcgcaa cttttacaat ttcaaactac gaattatgat
     1321 tcgtcatggc aaaacatttt taaccaaata aacagaacag aaagagg
//
LOCUS       DANKKG010000279         1364 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000279 DANKKG010000000
VERSION     DANKKG010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.285"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            82..>1364
                     /locus_tag="PW004_002886"
     CDS             82..>1364
                     /locus_tag="PW004_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HDL2727797.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          465 a          262 c          257 g          380 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taat
//
LOCUS       DANKKG010000280         1344 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000280 DANKKG010000000
VERSION     DANKKG010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.287"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            243..1273
                     /locus_tag="PW004_002887"
                     /pseudo
     CDS             243..1273
                     /locus_tag="PW004_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297164.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          529 a          149 c          228 g          438 t
ORIGIN      
        1 ttgacggata atacgctcac ttatccagac actcaaatga tttttctctt agtacctatg
       61 atagtttcgt gggttgataa tcaacaacga atatcggaat agatagctaa agtatatttt
      121 aattatgaat ttataattag ttgtaaaagt taatatttaa cggacaggaa gtagggtgaa
      181 aaaatttgaa aaaaatttta gtgttagcaa atacatatta tcaaataata gtagcaatac
      241 aaatgaaaaa tacaattttt tccgatgagt atgttgaaat tattatttct gatcattcta
      301 aaaacactaa agaaattgcg aaaaaaatag aagaaaaaaa agtgtttgat ggctgtcatt
      361 atttagaaag caaatctttt gatgataata ttgggtattt tcaaaagatt aaggactgtc
      421 ttcagatttc ttgtggtaaa tcaaatcgat atgcctatta tttgaaaaac tgtaaaagtg
      481 aatattttga tgaatttatt tgtttttcaa tgggagaggt ttggttggaa ggaatatatg
      541 cttgcttata tcgaaataat aaaaatgtac aggtatcaat tttagaagaa ggtattttaa
      601 gctatggagt tgacattatt ccaacagcaa gaacaaaagc tatcgcagta ctcaattact
      661 taaaaaaaag gactccacta tataacaaaa gctataattt ttattgtttt tttcctaata
      721 tatatacagg aagagagaat acaatacaaa ttccattagt tacagctggg tcaagaaccg
      781 cattgcaact ttcggatatt tttaacccta tgatagatat ttataaaaaa aaaaatatat
      841 attttttacg tctgtttatg attttgaggg cggagaacct gtcggagaat atgaattagt
      901 atgcaaaata gcagacttag taggaaaaga taatctaatt gttaagacgc atcctagaga
      961 tgtaagaaca atttataaag ataatggatt tatcgaggat aaaaattcat caataccttg
     1021 ggaggcgatt caattgtcag gcgattttaa cgataaaata tttatgacaa ttaattcagg
     1081 aagtgttatt tcaggtagta caatgtctgc aaaacctgta cgcacgtatt atatgtataa
     1141 attgtgtaac atagaaggaa atccgtcttg caaaaagaat gcgtatgata ttgagcgatt
     1201 gcttaagcaa aaaacgatga aaaacatttt aaagaatgtg aaaatagcag aaagcttaga
     1261 tgatatacta tgagcaaaac taaatattta attaaaaata tgggaatatt aacaataagt
     1321 aatttttctt caaaaatttt agtt
//
LOCUS       DANKKG010000281         1335 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.288, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000281 DANKKG010000000
VERSION     DANKKG010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.288"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            52..561
                     /locus_tag="PW004_002888"
                     /pseudo
     CDS             52..561
                     /locus_tag="PW004_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338071.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            562..879
                     /locus_tag="PW004_002889"
     CDS             562..879
                     /locus_tag="PW004_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HDL2727798.1"
                     /translation="MLSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYIIQKDK
                     VSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTAKGMGR
                     LIS"
BASE COUNT          489 a          212 c          283 g          351 t
ORIGIN      
        1 tttttgagcc aaaagtaagg aattacaaaa agagaaagaa gagaaggaag aatgaaaaag
       61 gtattatttg cggttagttt agttgttaat atagcactag caggaggatt attttatacc
      121 attacagaaa tgaataagct tgatgaccaa cgggaaagct tagcaatcag taacgaacaa
      181 tacaaaagga agctagatta agctagcagc accaacgtgg acactcaaga agccgtagaa
      241 tcctctacaa ttgtttatag caatgaatcc agtgaaaccc taacaaattc ggaaagaagc
      301 acagctccag ctacagaaac tgtttctcaa aaagctacta taacaatagg aagtaaagta
      361 ttcacgttta tagtaaatgg cttgacaata gaacacgatt ctagcagaac aggaaaaatg
      421 accgagcttt ctcacgagtt tccagtgatt gttactactc ctagtgctac ccttaacggt
      481 atattggtgc aagacaacaa tgataataat tgctatataa tggtagacgg tcaaactcta
      541 tacagaggaa cataccaata aattttgagt ctcaaagata tttgtcacta cggtaaacgg
      601 tgtaccgcta cagagaaaat cacgaagaaa ctatcaactg gccaaagtaa aaccgttgtg
      661 cagtgtaaga aatatataat ccagaaagac aaagtctcag aggaaatgat ttattacatt
      721 gggaaacaaa agcaaataat tttaaaagat cctatcccct taaaagagct atatccaaca
      781 atcaaacatg tttatgacca aaacggtgta ctgattggtc gaagaaaaaa tggagtcttg
      841 cgttgtaccg caaaagggat ggggcgttta atcagctgaa ttgatcattc tcataacttg
      901 tattttatcg tttttcgctt gcttgaaaat attgtcgaga cttctaaaag aacttagaat
      961 cattagaaaa ataaggagta gacttgatct atcaacaatt tccttcgggg agtgacgggg
     1021 tagattttta cgattggtaa cgtaaaaaat tggtcaatcg agtaaatgcg cttgtttgga
     1081 tagtggtgaa atgaatttgt gtaaataata tacacagagt aaacaagagg gaatttagag
     1141 gttagacaag ttagaatagc gcctagatta atcatgagca gctactaagg gatctaggct
     1201 ttgacgcaaa ctcatggaat aaggagtagg tcttctcgta atggggagga cgcacaagaa
     1261 attgtgtcgt gtgctgcagt acatgtagat acttgcgagg acgttggtca ttggatcaat
     1321 cgagcattta ccgct
//
LOCUS       DANKKG010000282         1334 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.289, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000282 DANKKG010000000
VERSION     DANKKG010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.289"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            42..452
                     /gene="spx"
                     /locus_tag="PW004_002890"
     CDS             42..452
                     /gene="spx"
                     /locus_tag="PW004_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HDL2727799.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            466..822
                     /locus_tag="PW004_002891"
     CDS             466..822
                     /locus_tag="PW004_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HDL2727800.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          486 a          191 c          250 g          407 t
ORIGIN      
        1 attactgtag aattggatta taattaaaaa ggagtgagaa aatgataaaa gtatatactt
       61 cgcctagctg tacttcctgt agaaaagcaa gagcatggtt gcaagcgaat caacttgaat
      121 ttgaagagaa gaatattttt gccgaaccgt taacagaaag tgagatcaaa aagatcttgg
      181 cagcgactga aggaggagtt caagatatta tttccactcg gtcgaaaatt tacgaaaagc
      241 tgggcataga ttttaacgaa ttaactttaa agcaaatgat cgtcttattc aaagaatatc
      301 cttcattatt gagacgacct attttgattg atgaaaaaag aatactaatc ggatttaatg
      361 aagatgagat ccggtcattt gtccctcgtg aaattcgatg tgttgccaga aatctgatct
      421 atttacaaaa caataacatc ttaaattcat agggagggag ccttcgtgga agtgaaagtc
      481 tatggatcag cgcactgtgt aacaagtctg aaggcgaaac aatggttaga acaccatcaa
      541 ttgccttatc aatttgttga cttagaagaa cgcgatttaa gtacagaaga agcagaagaa
      601 ttatgcagtt ttgaagaagt gcaagcagaa cagctatttg cgacttggtc tgaggctttt
      661 cacaacattg cgattgacct ttcctgtgta ggaaaaaaac aactgatttt tttatgcaca
      721 caacatacca atctcctcag acgtcctttg attattatcg atgaagttct atttattgga
      781 tacaatgagc agttgatgga acagcttatc ttaaataaat agactttaac tgttttaatc
      841 aagtcactga gcatttcata cagttgtaac agtcgaacac ttagcatcaa gcgtgttatg
      901 tgttcttttt ttgactttat ttttctacta tagagacatg ctcaatttat gtggtcaaaa
      961 ttcaaatcta ggtggggata tagagttaaa aatatattga catgtgtaaa tttacatgat
     1021 atattcattt ctgttgtttt aagtagcaaa gcaataaaat agtattttaa aaaacatata
     1081 aaatagttgt tgacatgtta gaaatgacat gataagataa ctgagttgtt aaaaaagaac
     1141 cacttgtgaa gcttgattct atcgaaaaaa acttcaaaaa agttcttgac aaataacaaa
     1201 tgataagtta tgataaatga gttgctgtaa gatttcgaaa aacgaaatca aaaaaacttt
     1261 taaaaaagag ttgacaatta cttgtctctc tgatatgata taaaagttgc tacggcagcc
     1321 aaaaacgaat cggg
//
LOCUS       DANKKG010000283         1330 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000283 DANKKG010000000
VERSION     DANKKG010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.290"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(30..914)
                     /locus_tag="PW004_002892"
     CDS             complement(30..914)
                     /locus_tag="PW004_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HDL2727801.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPEAINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSILLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(1009..>1330)
                     /locus_tag="PW004_002893"
     CDS             complement(1009..>1330)
                     /locus_tag="PW004_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727802.1"
                     /translation="HQFSVLVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIA
                     IRSDNKKWKKRYYILLLFLCFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWVFY
                     LVKK"
BASE COUNT          417 a          284 c          206 g          423 t
ORIGIN      
        1 ctgacaactc ttcggtcaca acctccattt taacaataaa cctgtaaatg tgagggtaac
       61 actttgattg tcacaggtaa tttatcccct tcatctccat caacattcgt cgtcaattct
      121 tcctcatcag ctaatgacac aactaattgc tcacaagtaa gataggctag atttttcgta
      181 gattcatcta ctccttttaa taggtctggc aatgttttaa gcgtatcgat caaagaagaa
      241 tcttttaaat aaaccaaatg gagcttacca tcatccactt ttgcatttgg aagtaatgtt
      301 tcaaaaccgc ctactgagtt agttagtcct atcagcaaga tactgctctc aatttcttcc
      361 ttctttccat caacttctac gtgaaacgag taggattgtg tggaagctaa ctgtttgatt
      421 cctgacatga aataagctag ttttccaaat ttcgtcttct tctccgcatc tacatcattg
      481 attgcttcag ggatagagcc aattgcgacg acattcataa aatagtcgtc atttattttt
      541 ccaatatcca atggcttgac tgattcaatc gaaaaatgat tgatcgcttc ctcaggttct
      601 agcggtatcc ctaatgctcg agccaagtca ttcaccgtac ccaatgggaa aaatccgaaa
      661 tttggccgat gttcttgttc agcgattcca ctgattcctt cgttgactgt tccatctccg
      721 cccatgacaa acacactatg atacccttcc gtagccgctt cgcgagtaaa gttttttgca
      781 tcccctgctt tttttgtatg caagacgacc acttcatcaa acaccgattc caattttgct
      841 atcgctaatt gctcaaattc ctttgcttgt tcaccgcctg aactggggtt taccactaat
      901 aaaacttttt tcatcacttt ctttcccttc tgattgattt ctttaatata acgatagcat
      961 aaagaaaaaa attaggctgt gatgaagtac tgttcatgaa acattcattt attttttgac
     1021 aagataaaat acccatacta tgaaactgag gaaaattaaa acaatgatat aggaaataaa
     1081 agaaaatgat aggcattgta tacctaataa agtaaaaatc ttaggtggca gataaaaaca
     1141 caaaaatagc aagagaatgt aatagcgttt cttccacttt ttgttatcag accttatagc
     1201 gatcagagga ataccaaatg cataactgaa aaactcttga tgtactgtta atatgtcacg
     1261 accttgaata ataaatgcaa caatccccgc tataattagt gtgcctaata ccaaaacgga
     1321 aaattgatga
//
LOCUS       DANKKG010000284         1303 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.291, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000284 DANKKG010000000
VERSION     DANKKG010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.291"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(58..672)
                     /locus_tag="PW004_002894"
     CDS             complement(58..672)
                     /locus_tag="PW004_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL2727803.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
BASE COUNT          414 a          258 c          220 g          411 t
ORIGIN      
        1 gctccatttt tatgtggaac attatttttt taaggaactt tgtcaacatg tggataacta
       61 ttttccactt gtgtcctgga ctagctgttt tgctgtaaaa tacaaaactt gctgcagcaa
      121 aaagatcgtt gcaagagagg ctgttccgca gatcgctgga tctagatttg gcaaagtcaa
      181 gactgcatca tgtaaattgg cgtggttgtc caaaaccaaa tcagcaaatt caaataatcg
      241 caaaccactg ctatgttgcg atttgatact tcgtgacaaa tcaaatccag taacaatgat
      301 taatggatga ccattttctt tgatccactg agctagttcg atgattgccg ggtttcttcc
      361 ttcattcgaa agaagaagaa aaatgtcttc tgatttgaca gaaagctgat caataattgc
      421 ctgtcctgca ccttctagcg tttcatagac acctccggct ggattttcaa tcaatatact
      481 tggtacaaga tccggggttt gatcccaaaa ttcaaatgct acaccactta gtgtgcggct
      541 agcatatatc tgcaatctac caccttgatt aatttgtttc gcgatccaag tgccagcttg
      601 aagcatcagc ggctcttcta tccttaattt ctcttccaat tggtgataat agtcaaaata
      661 acataaactc atttatctct cccgcctagc tatacttgac atgaatagaa acagctgcct
      721 aatctaagga agtacataaa aaaacagtag ttagacagta acacatgtgg tctttgctgt
      781 ttcaaacaca aaaaaggcat tgaacgaata cgcccaatac cttgacatag caaataaaac
      841 aacgtgcaga ccatggaaaa tatctacata ggggtagaat tttcacccta gattaggtac
      901 aataaattag gtcgatgcac tgcattgact atggcaaaat gatcggtcag ggtgtatgtg
      961 tgtgactggt cattccctga ttcttagcgc caactaaggg tcagggagcc tttttttatt
     1021 cagctgtttc tatctgaatt tattgtaagt aatctcttat ccaagtgcaa gcaagaaaat
     1081 acaaaaaaac attggtatga tttatatata tataaataac ataaaaaaag gaatggctta
     1141 tcccgataca acaagcattc ctttaaaaaa gattctttta tataagtgaa aaaagctttt
     1201 ttccaccaat taaataaatc tctcatctca atagtttatc cattatttta tctttttttg
     1261 cgtataccta ctaaatatta ccgtcttctt ctcgttcttc tac
//
LOCUS       DANKKG010000285         1303 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000285 DANKKG010000000
VERSION     DANKKG010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.292"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            6..398
                     /locus_tag="PW004_002895"
     CDS             6..398
                     /locus_tag="PW004_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDL2727804.1"
                     /translation="MFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIELNQ
                     VRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVEDK
                     EMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            382..1076
                     /locus_tag="PW004_002896"
                     /pseudo
     CDS             382..1076
                     /locus_tag="PW004_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
BASE COUNT          497 a          196 c          260 g          350 t
ORIGIN      
        1 tgacgatctt tgaaacggtt gtaacagatg ttggtataga agcagaagta ttcaggaaag
       61 aaaaaataat gattttattt ggagatgaag caccggaaac attaacagat tattgttata
      121 agattgaatt gaatcaggta agaaaagaaa taacttgtga tatgaagctc tgttttgata
      181 aacaaatgta tgtaatcaca gcagtaggag atgttgtcca aactaatttg aatgaattag
      241 gacatatcac gataaaattt gatggatctc atgagccgga acttccagga acaatgtatg
      301 tagaagataa ggaaatgccg catttaagaa aggggacaat cattaaaatc gatcaagagg
      361 atcagaatgg gggaaagcta tatggaaatc atgcttgata cagcaaatac aaaaagtatt
      421 tattcttgtc aacagttttt gaatttaaca ggcgtaacaa caaatcctac aattctgaaa
      481 aaagaaggag cgtcaaactt cttctcgcat atgaaagaaa tcaaaaaaat aatcggacag
      541 attcctatcc atattcagac aataggaaga acggaagaag aaatgataga ggatgctcga
      601 gttatcatag aagaactggg aaaagatacg tttatcaagg tgccggtgaa tcaagcaggc
      661 cttcaagcga tcaaaaagtt gaaaaaagaa ggttatttga ttaccggtac agctatttac
      721 acagaattac aagggtattt agctattaat aatggagcag attatattgc accttattat
      781 aacaggatgc aaaatcaagg cattgatgct atgaaagtga ttgattcgtt gcggactgag
      841 atcgaacgta cgcaaagacc aacaaaaata ttagcagcta gtttcaaaaa ttgtgaacaa
      901 gtcactacag ccattcgatc tggagctcag cgataacgtt aggtaaagag ctactagatc
      961 aagtattaga gatgccctcg attgataaag cagtgagggg cttccaagaa gattgggtgc
     1021 aaactttcca agcagaaacg attggtgaac taaaaaaggt cgctgattac ttctgaatca
     1081 atttctccat aaaataaacg atagccttct cgaaaactcg aggaggctat ctgacgattc
     1141 aatattcatt aatttaaagt ttgggtgact attactaact attacttatt tctcatttaa
     1201 acgatgtatt ccctaacctc taatacatcc ttgtaactct gaagttacgc cagttttgaa
     1261 taaatttcat aaaaacaaat gaaaagtgtc gcataaaaat atc
//
LOCUS       DANKKG010000286         1276 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.293, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000286 DANKKG010000000
VERSION     DANKKG010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.293"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(62..433)
                     /locus_tag="PW004_002897"
     CDS             complement(62..433)
                     /locus_tag="PW004_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727805.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(435..836)
                     /locus_tag="PW004_002898"
     CDS             complement(435..836)
                     /locus_tag="PW004_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727806.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            complement(850..1257)
                     /locus_tag="PW004_002899"
     CDS             complement(850..1257)
                     /locus_tag="PW004_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727807.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
BASE COUNT          408 a          227 c          161 g          480 t
ORIGIN      
        1 ttgatccaga aagatacaaa gattaaataa aaaaataaca aacaaactcg cttgaaattc
       61 attaggtgag tttgtttatt attttttctg cttctcgatt catcagcgcc atatctgttt
      121 tcgtaatggt ttcattagga atatagtcag cacattttct tttctctaac attctatacc
      181 cactattatg tattcctaag gtattcaatt ccttaactgt tgaaaacaaa ttccaaacgg
      241 tttggtggct tccttttccg ctacttccat tataaataat atcataattt tctttcaaat
      301 actcatcaga ttttttaaaa gcactgtagt atgctcttga aacagcagtt cttctataag
      361 attctttaga attatattta tcatctgatt caatttgttt ggaaatagat aaatattcat
      421 taaagtcaaa cattttattc ccccaaagtt gctactacag atttccccat atatttatag
      481 tcagataata cattatcgag agtatccaaa acatcgtaat ctgaggaaaa caattcagat
      541 gttgttttga taacaaagaa atacaagttt aaatcttcat catattttat attgatttca
      601 gcttttcttt ttaaaaccgt atttatcaca tcaaaaattt tgccatcatt acttgaaatt
      661 gtgttaatct cattttttgt agtaattaca agttgattat catttttagt tttcggagta
      721 gaacttgcat ccacaacaac tggagatgct agaattaccg atgcgcaagc aaaaatcgca
      781 gcaaaattta ttaagctacc actccgatgt gtatttgatg gtactatatc aagcatccgt
      841 atcgtctcct taattttttt ccactggttt gttgaccttg atatcaaaga tttgccttcc
      901 aatctcagtt ccattaaatt taacaacagc ataatgctct cctgcttctt caaccaagac
      961 atttctggcg tttatattaa agtttatatc gttaacttga ggaggcaagc ttaccgatac
     1021 ttcacccgta gaaaaaattt gtttttcatt cttgtttaaa acttcaattt ctactatttt
     1081 tatcttttct gtatcctcta tctcagacac cacagctgtt acagaaaatg ataaagcagt
     1141 aggaattaaa ggcgcattaa tagaaagagc tggattaatt actttattaa taacattagt
     1201 gccaaattgc tgactttcta catcttctga aattattaat tgtactctat aagacattga
     1261 tttctcctaa ttttta
//
LOCUS       DANKKG010000287         1237 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.294, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000287 DANKKG010000000
VERSION     DANKKG010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.294"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(1..567)
                     /locus_tag="PW004_002900"
                     /pseudo
     CDS             complement(1..567)
                     /locus_tag="PW004_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            588..>1237
                     /locus_tag="PW004_002901"
     CDS             588..>1237
                     /locus_tag="PW004_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit R"
                     /protein_id="HDL2727808.1"
                     /translation="MCTKLLQNTAHKNPYKTVDEATQLDLVIVVNQLLTGYDSKWVNT
                     LYLDKSLEYQNIIQAFSRTNRLNGPEKPFGTIRYYRKAHTMEQNIEEAIELYSGKQAK
                     GLFVEKLYNNLEKLNDIFEEIKSVFNISGIENFSHLPEVPGEIKQFVNLYNQLIHCYE
                     SAMIQGFTWAKSTYEIQDYEPITVVITEYEFNILAQRYSEIEREPRDGNDSIAYDI"
BASE COUNT          411 a          212 c          248 g          366 t
ORIGIN      
        1 taacgtagat gaaattgttt taagggtttc ttgaaagcct accgtatctc gtttaacgaa
       61 ataatagcgt aataattgac aaaaatgata cgcagttcgc aattcttcgt tgtagcttaa
      121 cagctcatta acgatttctg tttgactaat tggtcgttta aacagtcgat gataatgccg
      181 tttgagtgcc tcccatgtcg tggaatcttt gagtaagagt ttccaatatc gcttgagccg
      241 acggtacttc ttctgatttt cagagtgtga ggttcgaaaa cgattcatga tctggacacg
      301 taattgattg aagctgcgat tgatgtgttg gacaatatga aagcggtcag ttacgatctc
      361 cgcatttgga aagaccgttt taagcaactg acaatagctg gcattcatgt ccatcactag
      421 gtacttgacc tttaaacggg cctttctagg aaagcgcagg aagtattgag ttagtttgta
      481 aagtctgcga tcttcaaggg tcgtaaatag ctgattggtt gtcccatcaa cacaaataaa
      541 actcatggaa ccagaagtgg caacattgtg tacttttctt tgcaatcttg tgtacaaaac
      601 tattgcaaaa cacagcgcat aagaatcctt acaaaacagt agatgaagca acccaattag
      661 atcttgttat agttgttaat cagctcttaa cgggttacga ttcaaaatgg gtcaacaccc
      721 tatacttaga taagtcactt gaatatcaga acataattca ggctttttca cgcacaaatc
      781 gtcttaatgg tcctgaaaaa ccgttcggaa ccattcgtta ttatcgtaaa gcacatacga
      841 tggaacagaa cattgaagag gctattgagt tatactcagg caaacaagca aaagggttat
      901 ttgttgaaaa attatataat aacttagaaa agttgaacga tatattcgaa gaaattaagt
      961 ccgtatttaa tatttctggc atagaaaact tcagtcattt accggaggtt ccaggggaaa
     1021 ttaagcaatt tgtaaatctt tataatcaac taatccattg ttatgaatca gcgatgatac
     1081 agggattcac atgggcaaaa tcgacttatg aaatccagga ttatgaacct ataacagtag
     1141 taatcacaga atatgaattt aatatcttgg cacaacgtta cagcgagata gagagagaac
     1201 ctagagacgg aaatgatagt atagcgtatg acattga
//
LOCUS       DANKKG010000288         1214 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.295, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000288 DANKKG010000000
VERSION     DANKKG010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.295"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..>1214
                     /gene="aph(2'')-I"
                     /locus_tag="PW004_002902"
     CDS             <1..>1214
                     /gene="aph(2'')-I"
                     /locus_tag="PW004_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HDL2727809.1"
                     /translation="SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHY
                     PKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIR
                     AYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDS
                     IEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEY
                     SYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISEC
                     TIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCN
                     HLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEK
                     AKEYQDIVEEYY"
BASE COUNT          509 a           95 c          209 g          401 t
ORIGIN      
        1 aatcattaaa aaaacattat acagagcctt gggaagatga agtttttaga gtaattattg
       61 aatataacaa tgttcctatt ggatatggac aaatatataa aatgtatgat gagttatata
      121 ctgattatca ttatccaaaa actgatgaga tagtctatgg tatggatcaa tttataggag
      181 agccaaatta ttggagtaaa ggaattggta caagatatat taaattgatt tttgaatttt
      241 tgaaaaaaga aagaaatgct aatgcagtta ttttagaccc tcataaaaat aatccaagag
      301 caataagggc ataccaaaaa tctggtttta gaattattga agatttgcca gaacatgaat
      361 tacacgaggg caaaaaagaa gattgttatt taatggaata tagatatgat gataatgcca
      421 caaatgttaa ggcaatgaaa tatttaattg agcattactt tgataatttc aaagtagata
      481 gtattgaaat aatcggtagt ggttatgata gtgtggcata tttagttaat aatgaataca
      541 tttttaaaac aaaatttagt actaataaga aaaaaggtta tgcaaaagaa aaagcaatat
      601 ataatttttt aaatacaaat ttagaaacta atgtaaaaat tcctaatatt gaatattcgt
      661 atattagtga tgaattatct atactaggtt ataaagaaat taaaggaact tttttaacac
      721 cagaaattta ttctactatg tcagaagaag aacaaaattt gttaaaacga gatattgcca
      781 gttttttaag acaaatgcac ggtttagatt atacagatat tagtgaatgt actattgata
      841 ataaacaaaa tgtattagaa gagtatatat tgttgcgtga aactatttat aatgatttaa
      901 ctgatataga aaaagattat atagaaagtt ttatggaaag actaaatgca acaacagttt
      961 ttgagggtaa aaagtgttta tgccataatg attttagttg taatcatcta ttgttagatg
     1021 gcaataatag attaactgga ataattgatt ttggagattc tggaattata gatgaatatt
     1081 gtgattttat atacttactt gaagatagtg aagaagaaat aggaacaaat tttggagaag
     1141 atatattaag aatgtatgga aatatagata ttgagaaagc aaaagaatat caagatatag
     1201 ttgaagaata ttat
//
LOCUS       DANKKG010000289         1195 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.296, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000289 DANKKG010000000
VERSION     DANKKG010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.296"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..1076
                     /locus_tag="PW004_002903"
     CDS             <1..1076
                     /locus_tag="PW004_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HDL2727810.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          211 g          332 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tcttg
//
LOCUS       DANKKG010000290         1192 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.297, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000290 DANKKG010000000
VERSION     DANKKG010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.297"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(<1..175)
                     /locus_tag="PW004_002904"
     CDS             complement(<1..175)
                     /locus_tag="PW004_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HDL2727811.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDP"
     gene            complement(340..744)
                     /locus_tag="PW004_002905"
                     /pseudo
     CDS             complement(340..744)
                     /locus_tag="PW004_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
     gene            complement(803..>1192)
                     /locus_tag="PW004_002906"
     CDS             complement(803..>1192)
                     /locus_tag="PW004_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2727812.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          363 a          213 c          202 g          414 t
ORIGIN      
        1 ctggatcgat acttgggcga cctaaatcgg agtaagtggc ttcgacgatt ggataaataa
       61 attcaaaatc aagcgcggca tcaatctttc gaacgagatg atcttgagga actagatcat
      121 caatcgtaag catcgcaaat tgatttctac cattcgtatt gtttttagac atcatagcta
      181 tcactccttg cattcgtttt cataaatcta ttataaaaga aaagactgta gatatcatcc
      241 ataaaatgga ctttgtctac agtctgagac acgacctaaa gatcgtgtct aattatgact
      301 gtatcagaaa atatgatacc aaacggatct attcattaat taattgacat agtttatctg
      361 ttgctctttt atgactggat aactgaccaa ttcatgacga tccaagtctt ctttattcat
      421 atttacagca gtaatttgac tgtcttcata cgtacgataa taataaatcc ctttatcaac
      481 gttacagcaa gaagaataaa tcgtatattc gtattttcct tctccagtat cgcatactcc
      541 tttttgttgc tcgactgagc ctagtatatg aaaaaattga ctgatacttt cagattctga
      601 atcacctgat gcagcattca ttttagtaaa tgtggctttt acaaatcgag aaacagaaga
      661 gagatctcca ggtaagccca atcctcccat cccacgacta taaacattca agctgatctg
      721 gtttgaaaaa tgattttctg gtgttccatt gggagcgtca ataattttgt gtaaataaat
      781 tgtcctcctg caaaataatt agttactcag taaacattga aactaatgta tcggttacct
      841 gttgaaaacc tttatggctt ctgtttagaa atttttgatt gtatgtatca aaaatgctga
      901 ctagaaagcg ttctagtgat tcttcatttt gaaactgctc ttttctacgg ctgtatcttt
      961 taatttgctt attgaaagac tcgattagat tggttgagta aatggttcta cgaatgctag
     1021 gtggaaaatc ataaaaagtt aataagtctt ggttttctat gagtgactgc gtcactttag
     1081 gatagttttt cttccatttc tcaatcatgc cggataagaa ggtattcgct tcttcttttg
     1141 agttagcttg ataaacagcc ttaaagtcat cacagatttc ttttcggtct tt
//
LOCUS       DANKKG010000291         1188 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.298, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000291 DANKKG010000000
VERSION     DANKKG010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.298"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(1..254)
                     /locus_tag="PW004_002907"
                     /pseudo
     CDS             complement(1..254)
                     /locus_tag="PW004_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            complement(286..447)
                     /locus_tag="PW004_002908"
     CDS             complement(286..447)
                     /locus_tag="PW004_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HDL2727813.1"
                     /translation="MAYMVARTQKHKVGSLNGLQNHVDRKTKNHTNKDIDITKSGSVA
                     KFSKKSILV"
     gene            complement(771..1103)
                     /locus_tag="PW004_002909"
     CDS             complement(771..1103)
                     /locus_tag="PW004_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727814.1"
                     /translation="MNNREILAKEMADLKAKEAKIRAKRKQIRQKEMISLAKLYCKKE
                     GFKTPAEALDFLESNKRNHSVNDEVLVSLRKFADGLVWQENGAYWRTNDVKGLTQFMA
                     QFRSENVN"
BASE COUNT          346 a          249 c          156 g          437 t
ORIGIN      
        1 gtcccttaat tttgatatag gtttcgtcca ttttccatga atagaaggat tgtctatttt
       61 tcttcttcca aagataatcg aggactttgc tgtactcttg cacccaacga taaatcgtag
      121 tatgacaaac atttattcca cgatcatata acaattcctg aacttcacga tagcttagat
      181 tgtaacgcag gtagtaacta acagcgacaa taatgacgtc attttttgaa ttgtttgcct
      241 ttaaaatgat tcattactct atcctctctg tcttttttct caattttaca ctaaaataga
      301 ttttttggaa aactttgcaa cagaaccact tttcgtaata tcaatgtctt tattcgtatg
      361 atttttcgtt ttacgatcta catgattttg aagtccattt aacgacccaa ctttatgctt
      421 ttgtgttcgt gccaccatat aggccataac tgtgtacttc ctttcgttaa aattattgcg
      481 agaatcacaa gctaaattcg gaaaaacctc catgtaaggt ataacttact agacttatac
      541 tgcgtaaagt ctgtaagctc tgccgagggc tatatcggct taacagccga tgagaaactc
      601 cgtttctctc aaacgaaaaa gcgaacgaaa aacgactgcc ttcgcattcc ttttttacgt
      661 ccttttttcg ttcaaaacca ttggcttttt gcgtcattgt tttcacaatt tttattccgc
      721 aaaaaaccaa aagtgaaaag tattgctttt cacttcaacc cctcctcaat ttagttcaca
      781 ttttctgaac gaaattgtgc cataaattga gtcaaaccct taacatcatt cgtacgccaa
      841 tatgcaccat tttcttgcca aactaatcca tctgcaaatt ttcttagcga tactaaaacc
      901 tcatcattta ctgaatgatt tcttttattt gattccaaaa aatctaatgc ttcagcaggt
      961 gtcttaaagc cttccttttt acaatataat ttcgctaaag aaatcatttc tttctgtcta
     1021 atctgttttc gtttcgctct aatcttcgct tcttttgctt tcaaatccgc catttctttt
     1081 gccaatattt ctctattgtt cattttgatt cctcctaaaa ataaggcata tttctagttt
     1141 aaaatattta ttttacgaat agaggatttg aacaacgaat gacacaaa
//
LOCUS       DANKKG010000292         1186 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.299, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000292 DANKKG010000000
VERSION     DANKKG010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.299"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(146..1186)
                     /locus_tag="PW004_002910"
     CDS             complement(146..1186)
                     /locus_tag="PW004_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HDL2727815.1"
                     /translation="MTDFNFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCSVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFIPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAETESGISIIFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNSE
                     SFPKTITKDWLE"
BASE COUNT          337 a          187 c          169 g          493 t
ORIGIN      
        1 ttgtgatttt tatcataata ttttaactca taaattctat taaaatagct atttctttgt
       61 aacatttgta atataattag aataataaaa aatttttaat tttaatgcta ctccatgaag
      121 caacaatcaa tttgtcagtg aaatttcact ctaaccagtc tttcgtaata gtttttggga
      181 agctttcaga attcaaacgt ttttcacttg atactttagc agctattggt ttttcgataa
      241 accaattttt tacatagaca aaaatcaggt tctggatatt ttcaactttt tcggttttta
      301 atttttgata ggcttttaac agtgtcgtgt ataattcttg ttctgcattg acataataac
      361 tatcgagttc ttcaaaaatt attgaaatac cactttctgt ttcggctttt ttctttgcat
      421 tatgaatagt ctggacagtt ttttgagctt cactgtaact tgaagaaaat gtttttatta
      481 aatttaatac tcgttcaggg ataaacgtag caatgctgtc gtttgtcata attgtctcaa
      541 aattgtctaa caacaattga tcttgtaggc ggtctttttc agtgtctata ttgtgtctat
      601 tagtgtctaa tatattatta ttaagttctg gtatgatttt cataccctct gatttatcaa
      661 cgttttggca cggtatgatt ttcataccct cttgagcccc aagggattca gcgtctgatt
      721 tttcgtcgag ggtgggtatg attttcatac cctctgattt atcaacgttt tccctattct
      781 ctctatcaaa tttttcgagt aagtagatat cattttctga gacttttaat tctgctagat
      841 atagtctatt aggattattt ttccctaact gtttgttaaa tcccatctgc ttttgtaata
      901 ataaaccaaa ggattccaac tcttttttga ttgctatcgc tttttgtttt tggcagttta
      961 atacgctaca tagttcattt gtcgtataag taaaataaac attaccttct tcatcgattt
     1021 gccgtttgct gattgcgatc tctaatcttg cttttaaaag catataggca atcttagttg
     1081 aatctttcat ttttttgtat tcgtcagaaa caataaagac ttttggaaat tgataaaata
     1141 attcgttgta aactctatcc gccttaaaaa aattaaaatc agtcat
//
LOCUS       DANKKG010000293         1180 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.300, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000293 DANKKG010000000
VERSION     DANKKG010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.300"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..943
                     /locus_tag="PW004_002911"
     CDS             <1..943
                     /locus_tag="PW004_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HDL2727816.1"
                     /translation="CYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMTKN
                     ITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMILE
                     LIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYCHL
                     YDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSRDF
                     VYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEERS
                     GDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          380 a          178 c          252 g          370 t
ORIGIN      
        1 ctgttatctt atcaccggtg gggctggctt cattggttct accttagcca atcatttagg
       61 agaagaacat gtggtaatcg tcgtcgatga cttatcaatg ggaaaagtag aaaatcttga
      121 tatgacgaaa aatatcactt ttattgaggg agatgtcgct gagaaatcct tgatggaaga
      181 gatcatgaaa gctaataaat ttgattatat ctttcattta gcagcagttg ctagtgtggc
      241 agattcagtt gcccgaccag tagaaacaca tcgtgtgaat tttgaaagtg tattaatgat
      301 tttagaattg atccgcaagt atcaacctga tttaaaacgg atcgtgttta gttcttcagc
      361 ggctgtttat ggggatgaac cgactttacc aaaaaaagaa gagtccgtga ttcgcccatt
      421 gacgccttat gcggttgata aattcgctgc agaacaatat gtgctagatt attgtcattt
      481 atatgatgta cccggtagcg cagttcgttt tttcaatgtc tatggtccaa atcaaaatcc
      541 gaattctcca tacagtggtg tgatttctat tctcgtagat cgctataaga aacaaatcgt
      601 aggagaagca acttcattta cattatttgg tgatggaagc cagtctagag actttgttta
      661 tatagatgat gtaatccaag cattactttt agttgcgaat gaggaagctg cactggggaa
      721 acaattcaat gttggaacgg gtaaagcaac cacacttttg gaattgattc atgcaatcaa
      781 tgaaattctc ggtgtagaat tgacacttga atacaaagaa gagcgttcgg gtgatatcca
      841 tgactctcta gcagatatta ctaagatcca gtcaattggc tatcaaccga aatatggtat
      901 tttaagtgga atgactaatt atttgaaaac tgaaatcaat taaaacatac taagataaga
      961 ttagagggta aattcaggct ctttgtcaat aaggactgat gagttttgta aaatcaaatc
     1021 tgggatagaa gggctctttg tcaaatgatg ttggtaaaga gaaatcttag aataatttgg
     1081 acagaacaga aagaagaaaa cctctttctg ttctgtttat ttggttaaaa atgttttgcc
     1141 atgacgaatc ataattcgta gtttgaaatt gtaaaagttg
//
LOCUS       DANKKG010000294         1158 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.301, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000294 DANKKG010000000
VERSION     DANKKG010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.301"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            15..275
                     /locus_tag="PW004_002912"
     CDS             15..275
                     /locus_tag="PW004_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727817.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(637..876)
                     /locus_tag="PW004_002913"
     CDS             complement(637..876)
                     /locus_tag="PW004_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727818.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT          407 a          163 c          217 g          371 t
ORIGIN      
        1 aggatggatg aaatatgaat aacgaaacta tcattttagg aaaaaaatat atttgccaac
       61 ctattggctt gaaacaaaaa gtggttggag aagtaatcag taaattagaa aattgtgttg
      121 tgatctgcgt agacgaatat catgacgtcg atcgcaatga aatattagaa aaatgcggaa
      181 aagtagtggc aaaatatact cacgtttatg agttggaaac aacagaatat ctatataact
      241 atagcaacaa atatgaacat actttagttt cataaagatt taaataaagt gccttcaaaa
      301 gcgagaagac caagcttttg aaggcacttt atttagttgg cagtgattgt tcatgatttt
      361 tgatttattc aaaaggttcg cctagagaat ctgtctctgt aacgaggcct ggtggagaaa
      421 ctcgataaaa acctatactg ccagaactgt cctattttcg gatagacaaa ctagtagttt
      481 taaaaagatc atccgatcca atcatttcaa aaaatgcaaa actgatatca ggatcattta
      541 gctcaggctt gttatcgtaa agcatttgta aaggttgcat ttagttagga gtaaacgcga
      601 aactatcatc attatttgct gaagaagatg aagaagctaa ttcagagttt gtttgaaata
      661 aaggagacgt gctgacagtt tctttaccct gttttttgca gaggtagaaa cttttatatc
      721 ggaagtttct accggagaat ttttctcatt tgtagaaatt gttgaatcga tactattcga
      781 aatttgcgta tctgaaggta tgttctttga tgcagaagtc ccacaagcag taagtaacaa
      841 taaatttgat aaactgataa acagaattct tttcattatg tacacactcc ttatagtatc
      901 gtttagcata cacctatgct cagtattagg caaaatcaat gcaaaactat caagtctttt
      961 atagtatcta ttagtcttag taatacatat atggagaggg tgatatccca gttcaaacca
     1021 gatacattgg ttaccatctg tattaacgat tatgcctcta aacgcttttt ttatgaaaaa
     1081 atttataagt gaaaacgtag gtatgatatt atctagaggt aatttttttg ggtatttaaa
     1141 tagttgctag gaggatag
//
LOCUS       DANKKG010000295         1145 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.302, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000295 DANKKG010000000
VERSION     DANKKG010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.302"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(<1..1098)
                     /locus_tag="PW004_002914"
     CDS             complement(<1..1098)
                     /locus_tag="PW004_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HDL2727819.1"
                     /translation="MYLLNITKLWQDVYGLEKYIFLYYISYVWNQFLIQFAKGLEKVK
                     AMGIAGIISTIVTIIANIFFLLILKWGLIGFFIANILSQIISATYLSVLVRIWEYIDF
                     EAKDPQLKREMLNYSIPLIATVVGWWINSTADRYVVSFIVGVSANGLLSVAYKIPQII
                     NTLQGIFIQAWQISAIKEYGDNETAKFYGNTFVMINLLMCITCSSLIILTRPFSHILY
                     AKDFYEAWKYVPFLLIANVLNCASGLLGPILSAKKDSKSMMWSAIIGALVNVIMSVVL
                     VYKIGVQGATIATVICSYVIYVVRKRAVGKDIFIKHYHIVVESWGLLFVQAIIEIYFK
                     NYVIEIIIMIILIIINISYIKQLCYRGRNIIS"
BASE COUNT          431 a          211 c          128 g          375 t
ORIGIN      
        1 tgaaataatg tttcttcctc tataacacaa ctgcttaata taactgatat ttataataat
       61 taaaataatc ataattataa tttctattac ataattttta aaatatattt ctatgattgc
      121 ctgaacaaac aataatcccc aagattccac aacaatatga taatgcttta taaaaatatc
      181 ttttcccaca gctcgtttac gaacaacata tattacatat gaacatataa cagtggctat
      241 tgtggcacct tgaaccccta tcttataaac aaggactaca ctcataatca cattaactaa
      301 tgcccctatg attgcagacc acatcatgga tttagaatct ttttttgctg ataagatagg
      361 tcctaacaga cctgatgcac aatttaaaac attagctatt aataaaaatg gaacatattt
      421 ccacgcttca taaaagtctt tcgcataaag aatatgtgaa aacggtctag ttaatatgat
      481 aagacttgaa caggttatgc acattaacaa attaatcata acaaatgtat ttccataaaa
      541 ttttgcagtc tcattatctc catattcttt aattgcagaa atttgccagg cctgtataaa
      601 tattccctgc aaagtattaa ttatttgagg tattttatat gccacagaca ataagccatt
      661 cgccgagaca cctactataa aggaaaccac atacctatca gctgtactat taatccacca
      721 tccaacaaca gttgcaatta atggaatcga gtaatttagc atttctctct ttagctgagg
      781 atctttagcc tcaaaatcga tatattccca tattctcact aatactgaca gataagtagc
      841 tgaaataatc tgtgataaaa tatttgcaat gaaaaaacca attaatcccc atttcagaat
      901 aagcaagaag aaaatattgg caattattgt tacaattgtg ctgataatac ctgctatgcc
      961 catagcttta actttttcta accctttagc aaattgtatt agaaattgat tccatacata
     1021 ggatatataa tacaaaaaaa tatatttttc taatccataa acatcttgcc ataattttgt
     1081 aatatttaac aaatacatta cggatccaaa gactatcacg cttaaaaata taactttaat
     1141 cgcta
//
LOCUS       DANKKG010000296         1108 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.303, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000296 DANKKG010000000
VERSION     DANKKG010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.303"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            164..715
                     /locus_tag="PW004_002915"
     CDS             164..715
                     /locus_tag="PW004_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF334 domain-containing protein"
                     /protein_id="HDL2727820.1"
                     /translation="MKRLEASTSNFNNKLDVFEEKTKHARLNFETTAKHYIKRLDEDN
                     LKLDFQQAIQDELSDTKDEIREVTKQAREETKEYKEILESKIKDHNKVVDKSNTALKV
                     MTKGVTNIFFVLIIFVLVMLVTGPIGHFFGIEHLYSFINGFIDDHESAWRYLMLILYA
                     VPYVFFAIILWLIALFFRVFNDL"
BASE COUNT          416 a          158 c          212 g          322 t
ORIGIN      
        1 aaagaaggca ctctcttcgg gttttcggtc tgtactgaaa tcaaggtatt attgggaatc
       61 ccagcttaaa tcatagatac cgtaagggat tttattcttt atttaaaact ttgcaacaga
      121 accgaagagc gagcaagaca ggaacgagag aacaaacata ctcatgaaaa gactagaggc
      181 ttcgacctcg aactttaata ataagttaga tgtttttgaa gaaaagacta agcatgcaag
      241 attaaacttt gaaacaactg caaagcacta tattaaacga cttgatgaag ataatttgaa
      301 attagatttt caacaagcta ttcaagatga attaagcgat actaaagacg aaatacgtga
      361 agtgacgaaa caagcgagag aagaaacaaa agagtataaa gaaatcttag aaagtaaaat
      421 aaaagaccat aacaaagtgg ttgataaatc gaatacagcg ctaaaagtga tgacaaaagg
      481 cgttacaaat atattttttg tgctaattat ttttgtactt gtaatgcttg tgacaggtcc
      541 gataggtcat ttctttggta ttgaacattt atatagcttt attaatggtt ttattgatga
      601 ccacgaaagt gcatggcgat atttgatgtt gatactttat gcagtaccct atgtattctt
      661 cgctatcatt ctgtggctca tagcattgtt ttttagagta tttaatgacc tttaggtaga
      721 tttatatata aaaagggcta aatacgcaaa ataccccctt aaaatgcaaa ataaatgcaa
      781 ataagggggt aactccttat gaaaaaacaa ctaatagtat tataacacgt ttttatgccg
      841 agaaaactta ttggttggaa tgggctatgt gttagctaac ttgttagcga gttggttgga
      901 cttgaattgg gattaatccc aagaaagtac caactcaaca acacataaag ccctgtaggt
      961 tccgaccaat aaggaaattg gaataaagca acgaaaggag ttgagtaaaa tgagtgagaa
     1021 aacgaagaaa atcattcgag tatcattagc tttttcacaa gtagtagtgc aatcattgat
     1081 agtttattat acttaccagc aatataaa
//
LOCUS       DANKKG010000297         1107 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.304, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000297 DANKKG010000000
VERSION     DANKKG010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.304"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(4..402)
                     /locus_tag="PW004_002916"
     CDS             complement(4..402)
                     /locus_tag="PW004_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HDL2727821.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(399..635)
                     /locus_tag="PW004_002917"
     CDS             complement(399..635)
                     /locus_tag="PW004_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDL2727822.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            complement(795..1079)
                     /locus_tag="PW004_002918"
     CDS             complement(795..1079)
                     /locus_tag="PW004_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727823.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
BASE COUNT          343 a          201 c          174 g          389 t
ORIGIN      
        1 cgttcatttt attgcggttt gtcgtaaata actggttatc ttggacttta gtgaatcgaa
       61 atccaatgaa ctagtggtaa tcattatgat aaaatcaaca gcttcttgac gagaaaaagc
      121 aactggacaa ttattcaatt gaagaaacaa caacatggca acaagagcag ttcgtttatt
      181 gccattatga aaaggatgtt ttttagctaa attaattgct aaaatcgccg ctttttcata
      241 gatttcagga tagagttctt gattgaatac tgcttgtgaa ggctgtttga cggccatatc
      301 taatgcattt gcatctttta caccgatcat ttcgcctggc gagtgacgta aaataacttt
      361 cgcgttgatt ttgataattt ctttagtcga aagatacctc ataaatcaac caaatctttt
      421 aacgcgtctt catattgatt catgatcgaa tcaactaatg aatcaatatc aggttcttct
      481 tgtgctttga tcattcgtac agaaccatca gattgaaaaa cataactgat tgcgtcacct
      541 gtctgcacac ccatgaattc ttttacttct ggtgggatag ttgttacatc gctattgcca
      601 gattttctaa ttttatactt tttcattttt ttcatatttc gccaccctct ataccattct
      661 cattctatag taagtacatt atatccaagt atatactttt aatcaatgta tatactttta
      721 ttttgctttt tcttacgaaa aaatgacaaa gaaaaaagtt tgtgacatga cttttgtcac
      781 aaactctcgt attcttaacc aaaatattga tgaaatactt gtgtaataac tggtagaaaa
      841 taaataatga tcatagccgc taccaccacc aacaattgtt ttcgctgttt ctttcgcttt
      901 agcatgcttg tcgttttatg aaaaaaataa gtagcgcttt tagctcctgc agtcaaagtc
      961 gataaaatca acgttggtga atatgctgtt tgattgtgcg tcaatgacaa caacttcgtc
     1021 ccgtaatcag gatactttag aaatgcttgg tacacagcca caatcgtgat tgctcccata
     1081 agataattta aaaatgaatg cactttt
//
LOCUS       DANKKG010000298         1105 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.305, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000298 DANKKG010000000
VERSION     DANKKG010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.305"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..1087
                     /locus_tag="PW004_002919"
     CDS             <1..1087
                     /locus_tag="PW004_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2727824.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT          402 a          192 c          214 g          297 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaa
//
LOCUS       DANKKG010000299         1068 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.306, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000299 DANKKG010000000
VERSION     DANKKG010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.306"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            577..1008
                     /locus_tag="PW004_002920"
                     /pseudo
     CDS             577..1008
                     /locus_tag="PW004_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297423.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          374 a          149 c          183 g          362 t
ORIGIN      
        1 atgaaactag gcttttaaca ataattaggc aaaagtcgct catcctctac aggtgggtga
       61 ttaatgccgc tcgatatggt tgttaaccgt cggttactcg aagattgata tataagactt
      121 agtacctttt tattttgctc catgttatgg ccgaaggtct aaagatacaa tcattagtct
      181 agagaaaaat taagaaccgt aggaactacg gggatcgctt ggtgaataag agacactgct
      241 attagtgaaa aaataagata tcaagtacgc tctattccca aaaatttcgt gactttactc
      301 atgagaggtt caagtagtat atttttaatc aaaattgacg taacttttaa taaatactac
      361 ctaaataagg tagtatttat ttttgtgttt taaaagagga agtccaatat atctatggga
      421 aacttcttaa atattatgtt ggtacatgaa aatatacatc cctgctagac tatttacaac
      481 attttacttt gtacgtattc ttgttttaaa aggaatggga taaaatatag gtacaataac
      541 tatttgatgc taaatataat taatggagga gagatagtga acatacttaa tgaaaaaaga
      601 tatgtatggt ttgctggtaa aaaatatatt actactttca aaagattatc cgagttaatg
      661 cagcagactg ttaaaagtgc tatgcctgaa ggatacaagt ttaattttgt tcaatcttca
      721 gtatctactg aaagtatcta tttttcaatt ctttctgaga gtaggctttt tcattttaga
      781 ataagttctc atgtaaagaa aactgatgtt gatatatata cgttccgtac aaataattat
      841 aaaaatttaa atgatttgag tagaagtatc agtgaatatc tcaaggggaa tagctatctc
      901 aagattcgat tttcacatat attaatttta ctaccatatt tgaatgagaa tttcacccta
      961 attcaagtag ataagtcttt cattgcctca ggtaatcaag gaaatatgaa ggttctgttg
     1021 cgaagtttga aaatcaaacg cgccctctct gaactccaaa tatcttag
//
LOCUS       DANKKG010000300         1061 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.307, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000300 DANKKG010000000
VERSION     DANKKG010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.307"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(36..992)
                     /locus_tag="PW004_002921"
     CDS             complement(36..992)
                     /locus_tag="PW004_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727825.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          346 a          177 c          160 g          378 t
ORIGIN      
        1 tagaaaaagc agtcggactt tctaaaagaa gttcactatt cgatcctgtc gatgatttct
       61 tcaggaaaat cgtctatttg cttgtcaatt ttgctgtctt tgtatttctc ttcttggata
      121 gtcaacaaaa tctttgtaag attcccccag tctactgctc ttggcggatc gagccaaacc
      181 actacttgag atgcactttc ttcatataga ttattcctat tcgtgattat aacatccggg
      241 aactcaggct ttcctgcctc tatcacttcc gtgttcagat taatgtacaa gcttaaattt
      301 ttcttgataa attgattata ggcagcacca taagaaaaat cgatgatgat ctttactggt
      361 tcagaaatca gttgaatggg tatgtgattg atcaataata ataaataact gaaaaaaaga
      421 aaaaatttat cttgaagcaa ctctttgcgt tttgacgtta gatcatttat atactctatc
      481 aaatagcaat aaaatgctgg atagttttct ctaaaaaaag aaacatcaag tggaaattca
      541 tcagctttat taatagtgta atgcaaaagt ttaaaatgta ttttggttaa ctctcttcgg
      601 atcaattggc tcgtctcttc tcgcaacaga ttaaactgat tttcaaaact ttcaatgaat
      661 cgttcattca accattcgat tgtttcatct tcattttttc tcaagtattt ttgatccaat
      721 ttatcatgaa gataaagcca agagagtgtc ttattgataa aaataacgga tttttctcct
      781 aatactcctt ttaagtggta aaatcctata tccgaaaaaa atagcgactg attgctagca
      841 atatctaaag aatcagcttg ttcagccaac aggttgattg ctaaaaagtg gaataatttc
      901 tttttttcat aagcagacag aggataatac tgttttaaat cttttaacat tttttctgtt
      961 tttataatta taggtagcgt atacaattcc acaaaactta tctctgttga gttaaaaaga
     1021 ttatataaaa aaagccgaat gtttttagga ttccctgtca a
//
LOCUS       DANKKG010000301         1045 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.308, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000301 DANKKG010000000
VERSION     DANKKG010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.308"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            6..980
                     /gene="bsh"
                     /locus_tag="PW004_002922"
     CDS             6..980
                     /gene="bsh"
                     /locus_tag="PW004_002922"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HDL2727826.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          376 a          132 c          182 g          355 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgtaggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaac
//
LOCUS       DANKKG010000302         1044 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.309, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000302 DANKKG010000000
VERSION     DANKKG010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.309"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            243..905
                     /locus_tag="PW004_002923"
     CDS             243..905
                     /locus_tag="PW004_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2727827.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
BASE COUNT          354 a          184 c          179 g          327 t
ORIGIN      
        1 tctacttctt tttgagataa ttcagggata aagttcctcc agttgtttaa atcaatcatt
       61 tttcccctcc taaactaaca tattagtttt tgtattcaag tgaattttat gcaataaaaa
      121 tcgctatgtc aaccaaaatt ttactattta tacaaattta ctagttaatg tgacatacca
      181 gattgttttt cagacctctt ctgttatact acgttcatag aaatgatttt ggaggaataa
      241 agatgaaaag cagcttgcaa tctcaagctt atcaagcgat tcgtaaacaa attatctatg
      301 ctgacttaga acctggaagg aaaatatctg aaaaggcact ggaggaacat cttgctatcg
      361 gccggactcc cattcgcgaa gcattgattc aactacgtca gcaagaactc gtgtatacga
      421 tacctcaaag tggtacgtat gtttcaaaaa ttaatcttgt ttctgcagaa aatgctcgct
      481 ttgtccgaga acagttggaa gagcaaatca tgattgaatg ttgttccaaa ttggacacac
      541 aaacacgaat agtgctagaa acgattttag aagaacaaga aaaagcagtc aaaataaatg
      601 acgaacgcgc ttttttccgt gcagataatt tatttcatga agcttgtttc gaaattgcag
      661 gaagaaaaga agtttggaac tggttagatg accataatac ccatttagaa cgtttccgct
      721 ggttacgagt gaccattcag gggcttccat gggaaacgat catggaacaa cattatgatt
      781 tactcaaggc tttagttgat aaaaaaccag aagaagctcg atttcaaacg ggattgcact
      841 tacacatgat gctgaaagaa caggaaactg ttactcaacg ttatcccgag tatttccagc
      901 agtagcattt tcttgcgacc tcttctattt ttataattaa ccttcgtttt tgagtaatct
      961 ttattaaata acaaaatatc cattgacttt gcaactccaa ctaaattaca ataattataa
     1021 ataaataatt attattggag gttt
//
LOCUS       DANKKG010000303         1035 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.310, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000303 DANKKG010000000
VERSION     DANKKG010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.310"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(<1..>1035)
                     /locus_tag="PW004_002924"
     rRNA            complement(<1..>1035)
                     /locus_tag="PW004_002924"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          218 a          324 c          234 g          259 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc gggtccatcc atcagcgaca
     1021 cccgaaagcg ccttt
//
LOCUS       DANKKG010000304         1022 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.311, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000304 DANKKG010000000
VERSION     DANKKG010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.311"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            105..482
                     /locus_tag="PW004_002925"
     CDS             105..482
                     /locus_tag="PW004_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727828.1"
                     /translation="MKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRFEVLKIQKNE
                     LLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTDVVMITDIYV
                     EDEDIIKYMKPVIKLVNKNKGNG"
     gene            588..851
                     /locus_tag="PW004_002926"
     CDS             588..851
                     /locus_tag="PW004_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDL2727829.1"
                     /translation="MAKTVLNPSTARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            851..>1022
                     /locus_tag="PW004_002927"
     CDS             851..>1022
                     /locus_tag="PW004_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HDL2727830.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRY"
BASE COUNT          392 a          123 c          206 g          301 t
ORIGIN      
        1 acaaaacgtc acgtgtgcta ttggcgttga tgggttacgg aattctttat agagatgacc
       61 aaaatggcca gattataata actcgggccg ggcgttcatt attaatgaaa tatagaaaat
      121 ttataccgga agattttcaa aacttacagt tagaaaacat gcgtttagaa gatttattta
      181 gattagcaga agatgataag tatagatttg aagttttgaa aattcaaaag aatgaactat
      241 tgaataagtt atatcctgag aatgcgataa aagcacattt tggtgatgct aatcatatgt
      301 atagttggtt cattcctaaa agatttagag ggaaaatcaa tgctggagat caagtgaaag
      361 tactcaatcg aaatagtaca gatgtcgtaa tgataacaga tatctatgta gaagatgaag
      421 atataatcaa atatatgaag cctgtgataa aacttgttaa taaaaataaa ggaaacggat
      481 aacactttac aatgctgttt tggtactcca atttaaacaa aacaatcgaa tttacgtact
      541 taattaagta cgttagaatg gagatataaa ttagaggggg agtagatatg gcaaaaactg
      601 tcttaaatcc tagcacagca cggaaaaatt tttataagtt gttgaaagat gtaaatgaga
      661 atcatacgga aattgaaatc attagtgagc gcagtgaaaa taatgcggtg cttattggtt
      721 tggatgattg gaaagcaatc aaagaaacgc tattacttga gcaaataggc actttgggtg
      781 ttgttcgtaa gcgagaaaaa gatgatagtg gatttactaa tgttgatgaa attgattggg
      841 ataacttata atgaataact attctgtgat gattaaaaac tcggctaaag ctgatttaaa
      901 gaaaatcaaa cagtctaatt tgaaacctca atttgagaag gttattcaga ctttaaagga
      961 agatccctat ctccctaccc aatcatttga gaagttaaaa cctacacatg aaggtaggta
     1021 tt
//
LOCUS       DANKKG010000305         1011 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.312, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000305 DANKKG010000000
VERSION     DANKKG010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.312"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            131..556
                     /locus_tag="PW004_002928"
     CDS             131..556
                     /locus_tag="PW004_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727831.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
BASE COUNT          359 a          168 c          171 g          313 t
ORIGIN      
        1 tgccttttta aaattttatt ctaaaattta ctctatttgt gggtgttttg tgcgtttttc
       61 tcttccatcc gtttgaagtt ttacattcca atggtataat ttctttatcc tatcaaatgg
      121 gggaagtatt ttgaagaaat taatcgtact atttattttg tctgcgggac ttttgacatt
      181 gactggctgt gatttgtttg gcggctctga ccaatcctcg gacgaaacaa ccgattcttc
      241 tgtcttaaaa gcggaaaaag cagataccac caacagtgta tatacgctaa aagtacgtaa
      301 agatatggat ttatccgcgg gacagttgac atttacagat aaaaaaatgg aatggacacg
      361 cacgtatcag acacaaggaa ctgattcttc tgaatcatct gaagaaaaaa cagtgctgac
      421 agatatcaag atcactacca aagatgacga atatattatt tcaggaaaag aaaataaaaa
      481 agaaacaaca gtcatattta caaaaatcgg taattaccgt ttgaaggatg aggatggcaa
      541 catctacagt ctatagtgcc gtctgttgga tcttcttttg tttctcccct tcttttctgc
      601 tatagtaaag aagcaagatg attgtaaagg agacgaaatg gatgatcgat agtcctgatt
      661 attgtacatg taataacaac aaaaatatcc atacagaatt taatgaccaa ggaagcttga
      721 gcatatgcga caactgtcac aaactagtca aagattcatt taaaacattt gaatcttatc
      781 ctgttcaaaa ttatgtaaaa agtagaagaa gataaacaaa tccaagcaaa actcactatg
      841 gctgattttt atgaacttca aactcagctt cttcctttga tcaagcttca tcagtaaaac
      901 aaacagtgga tcatttcagt ttacaaatca aagagtaaag atttatttat ttcaaatatt
      961 tgaatataaa ataaccaaac gatagaaagg cttattctac atcacgtagt a
//
LOCUS       DANKKG010000306         1003 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.314, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000306 DANKKG010000000
VERSION     DANKKG010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.314"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..>1003
                     /locus_tag="PW004_002929"
     CDS             <1..>1003
                     /locus_tag="PW004_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HDL2727832.1"
                     /translation="VDKYVAVVTNPPYLNKMDKKLKKYVNDNYSDVKKDLFSVFIKLN
                     SQMLIDNGYASFMTPFVWMFISSYEKLRSFLINERNISSLIQMEYSAFEEATVPICTF
                     TIKNSNEQDGSYIKLSDFKGGMEVQKIKTLEAIHQHGECDYFYSTNQDNFKKIPGMPI
                     AYWASDNVITDFAQGKLTNDVADAKQGLATGNNNKFLRYWFEVEFNKIKFDAKTLEDA
                     KKSRKKWFPTTKGGRFRKWYGNNEYVVNWENDGDEIKNYVNENGKLKSRPQNLNYYFK
                     KGITWSTLSSGQVSFRLQNQAIFETKGSVLFSKDITDFEEIFGYVNSKLVNHFLTFIS
                     "
BASE COUNT          378 a          126 c          179 g          320 t
ORIGIN      
        1 gttgataaat atgtagcggt agtcactaat ccgccttatc ttaataaaat ggataagaaa
       61 ttaaaaaaat atgtaaatga taactattct gatgtcaaaa aagatctatt ttctgtgttt
      121 attaaattga atagccaaat gctgattgat aatggatatg cttcctttat gacgccattt
      181 gtttggatgt tcatttctag ctatgaaaag ttacgctcat ttttaattaa tgaaagaaac
      241 atttctagtt tgattcaaat ggagtattca gcttttgaag aagcaaccgt tccaatttgt
      301 acgtttacga taaaaaattc aaatgaacaa gatggaagct atataaaatt atcagatttt
      361 aaaggtggaa tggaagttca aaaaattaaa acgttagaag caattcatca gcatggtgaa
      421 tgcgattatt tttatagtac taatcaagac aacttcaaaa aaatcccagg aatgccgatt
      481 gcttactggg caagcgataa cgtaatcact gattttgctc aaggaaaatt aactaacgat
      541 gttgcagatg caaaacaagg gttggcaacc ggaaataaca ataaattttt gcggtattgg
      601 tttgaagtag aatttaacaa aataaagttt gatgctaaaa cattagagga cgctaaaaag
      661 tctagaaaaa aatggtttcc tacaactaaa ggtggtcgtt ttagaaaatg gtatggaaac
      721 aatgaatacg tggttaactg ggagaatgat ggagatgaaa taaaaaatta tgttaatgaa
      781 aatgggaagt taaaatctag accacaaaat cttaattact attttaaaaa gggaattact
      841 tggtcgacac tttcatctgg acaagtatct tttagattgc agaatcaggc aatttttgaa
      901 actaaaggat ctgttctgtt ctcaaaggat ataactgact ttgaagaaat atttggctat
      961 gtaaactcta aattagttaa tcacttttta acttttattt ctc
//
LOCUS       DANKKG010000307         1000 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.315, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000307 DANKKG010000000
VERSION     DANKKG010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.315"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            369..1000
                     /locus_tag="PW004_002930"
                     /pseudo
     CDS             369..1000
                     /locus_tag="PW004_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          181 c          183 g          294 t
ORIGIN      
        1 tttcaaagtt agtagagaaa atgtagttat atatttaaat ttttaccaga aagaaatgaa
       61 caccttcaat tatctaaatc tcgaaaatgg ttagatgtaa tcatcaggaa aaacaatctt
      121 aaacgaatta ctattcatgg tcttagacac acacatgcta gcttgctttt agcaagctag
      181 tgcaaatatt aaagatgtgc aagagcgttt aggccactct tctattcaaa tcattatgga
      241 tctatatatt catattacag acaaaagaaa agaagaaacc gtagcgcaat tcgcaaaata
      301 taggctctat aatttatggg actgatgaat caaaattgat tcttcagtcc cattgttatt
      361 ttaggtatat gattcgtaca ttgaaagagt tgaaaaacta tcttccgaag ccgtcaaaaa
      421 ggaccctgcc aaaagtattg atggtggatg aatttcgttc gcacgcttca attgtggata
      481 aaatgagctt tatttgtgcg gatggcgaga ctggaaaact cattgatatt ttgcctacac
      541 gaaaattgtc tcgattaaca cactatttta gtcgctgtgc aaatcctgaa gcggtggaat
      601 atttagtgac agatatgaac gccgcctact tccaactgac aaaacgtgtc ttaccaaatg
      661 cgaaagtcgt cattgattgt tttcatatca tcaaacattt gaatcaggcg ttcaatgatt
      721 ttcgtgtaca agaaatgaaa agattacacc agttagggca acgacgggaa gccgagaaag
      781 tgaaaaagaa ctggcgcttt cttctaaaaa accgtcaaaa aatcaatcac tatgattaca
      841 aaacatggcg cagttttcgt gcacctaaat acccgctact cactgaagca atgatgattg
      901 atcgattgct ggcattttca acgcctttaa aggacgccta tacttttttc catgagctga
      961 ccgaagcctt tagagacaag gaatcagact tattttttac
//
LOCUS       DANKKG010000308          996 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.316, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000308 DANKKG010000000
VERSION     DANKKG010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..996
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.316"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..>996
                     /locus_tag="PW004_002931"
     rRNA            <1..>996
                     /locus_tag="PW004_002931"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          268 a          227 c          301 g          200 t
ORIGIN      
        1 gaaataatag taccgaagtt cctgatgtca cactgccgag aaaagcttct agtgagaaaa
       61 cagcggcccg taccgcaaac cgacacaggt agtcgaggag agaatcctaa ggtgagcgag
      121 agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga
      181 tcatacgatc agccgcagtg aataggccca agcgactgtt tatcaaaaac acaggtctct
      241 gcaaaatcgt aagatgaagt ataggggctg acgcctgccc ggtgctggaa ggttaagagg
      301 agtgcttagc gcaagcgaag gtacgaattg aagccccagt aaacggcggc cgtaactata
      361 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa
      421 cgatttgggc actgtctcaa cgagagactc ggtgaaattt tagtacctgt gaagatgcag
      481 gttacccgcg acaggacgga aagaccccat ggagctttac tgtagtttga tattgagtgt
      541 ctgtaccgca tgtacaggat aggtaggagc cgtagaaatc ggaacgctag tttcgatgga
      601 ggcgctggtg ggatactacc cctgcgttat ggccactcta acccgcacca ctaatcgtgg
      661 tgggagacag tgtcagatgg gcagtttgac tggggcggtc gcctcctaaa aggtaacgga
      721 ggcgcccaaa ggttccctca gaatggttgg aaatcattcg aagagtgtaa aggcagaagg
      781 gagcttgact gcgagaccaa caagtcgagc agggacgaaa gtcgggctta gtgatccggt
      841 ggttccgcat ggaagggcca tcgctcaacg gataaaagct accctgggga taacaggctt
      901 atctccccca agagtccaca tcgacgggga ggtttggcac ctcgatgtcg gctcgtcgca
      961 tcctggggct gtagtcggtc ccaagggttg ggctgt
//
LOCUS       DANKKG010000309          991 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.317, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000309 DANKKG010000000
VERSION     DANKKG010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.317"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(21..94)
                     /locus_tag="PW004_002932"
     tRNA            complement(21..94)
                     /locus_tag="PW004_002932"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ile,seq:cat)
     gene            complement(108..181)
                     /locus_tag="PW004_002933"
     tRNA            complement(108..181)
                     /locus_tag="PW004_002933"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(145..147),aa:Met,seq:cat)
     gene            complement(209..281)
                     /locus_tag="PW004_002934"
     tRNA            complement(209..281)
                     /locus_tag="PW004_002934"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(246..248),aa:Ala,seq:tgc)
     gene            complement(301..374)
                     /locus_tag="PW004_002935"
     tRNA            complement(301..374)
                     /locus_tag="PW004_002935"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(338..340),aa:Pro,seq:tgg)
     gene            complement(384..457)
                     /locus_tag="PW004_002936"
     tRNA            complement(384..457)
                     /locus_tag="PW004_002936"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(421..423),aa:Arg,seq:acg)
     gene            complement(468..553)
                     /locus_tag="PW004_002937"
     tRNA            complement(468..553)
                     /locus_tag="PW004_002937"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(517..519),aa:Leu,seq:taa)
     gene            complement(570..641)
                     /locus_tag="PW004_002938"
     tRNA            complement(570..641)
                     /locus_tag="PW004_002938"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(607..609),aa:Gly,seq:gcc)
     gene            complement(653..725)
                     /locus_tag="PW004_002939"
     tRNA            complement(653..725)
                     /locus_tag="PW004_002939"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(690..692),aa:Thr,seq:tgt)
     gene            complement(743..824)
                     /locus_tag="PW004_002940"
     tRNA            complement(743..824)
                     /locus_tag="PW004_002940"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(788..790),aa:Leu,seq:tag)
     gene            complement(832..904)
                     /locus_tag="PW004_002941"
     tRNA            complement(832..904)
                     /locus_tag="PW004_002941"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(869..871),aa:Lys,seq:ttt)
     gene            complement(908..980)
                     /locus_tag="PW004_002942"
     tRNA            complement(908..980)
                     /locus_tag="PW004_002942"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(945..947),aa:Val,seq:tac)
BASE COUNT          260 a          283 c          235 g          213 t
ORIGIN      
        1 tgaaacttac aacgccacaa tggaccttgt aggactcgaa cctacgaccg gacggttatg
       61 agccgtctgc tctaaccaac tgagctaaag gtccaggctc ttaaaaatcg cggcggaggg
      121 gatcgaaccc ccgacctccc gggtatgaac cggacgctct agccagctga gctacaccgc
      181 gaaaaagata ttcattattt aaatgaaatg gagcctagcg ggatcgaacc gctgacctcc
      241 tgcgtgcaaa gcaggcgctc tcccagctga gctaaggccc cgtgtttaaa tttataatta
      301 tcgggaagac aggattcgaa cctgcgaccc cttggtccca aaccaagtgc tctaccaagc
      361 tgagctactt cccgtttaat taacgcgccc aagaggagtc gaacccctaa ccttttgatc
      421 cgtagtcaaa cactctatcc agttgagcta tgggcgcata tttgaaatgc cgaggaccgg
      481 aatcgaaccg gtacggtgat cactcaccgc aggattttaa gtcctgtgcg tctgccagtt
      541 ccgccacccc ggcgcgattg ctttggcaaa gcggaaaacg gggttcgaac ccgcgacccc
      601 caccttggca aggtggtgct ctaccactga gctatttccg cgtgtttaaa gatgccggct
      661 aaaggacttg aaccctcgac cctctgatta caaatcagat gctctaccaa ctgagctaag
      721 ccggcatgta aaacttaaat tatgcgggtg aagggacttg aacccccacg ccgtaaggcg
      781 ctagatccta aatctagtgc gtctgccaat tccgccacac ccgcaaaaat atgagccgta
      841 cagggctcga acctgtgacc ctctgattaa aagtcagatg ctctaccaac tgagctaacg
      901 gctcatatgg aggttaacgg gatcgaaccg ctgaccctct gcttgtaagg cagatgctct
      961 cccagctgag ctaaacctcc atggataact t
//
LOCUS       DANKKG010000310          989 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.318, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000310 DANKKG010000000
VERSION     DANKKG010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..989
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.318"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            55..989
                     /locus_tag="PW004_002943"
                     /pseudo
     CDS             55..989
                     /locus_tag="PW004_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          381 a          165 c          188 g          255 t
ORIGIN      
        1 gacagaaaaa ttaaggtgtg tagactagaa gaaaacatac caggaggaat ttttatgtct
       61 aagagaacac gaagaacttt ttcacaagaa ttcaagcaac aaatcgtcaa tctttactta
      121 gctggaaagc cacgtgtaga aatcattcga gaatatgaac taacggcttc agcatttgac
      181 aaatgggtaa agcaatctaa aacgagtggt tcattcaaag aaaaagataa tcttacgcct
      241 gaacaaaaag aattgttaga actacgtaaa agaaaccagc aattagaaat ggaaaatgat
      301 attttaaagc aagcagcgct gatattcgga cgaagagaca agtaatcgat gcgaataagc
      361 atctttaccc tatatcagcg atgtgcagaa tattaggtct atcacgtcag tcctattatt
      421 atcaatcaaa accaaagaaa gacgaatcag aacttgaaga agtagtcgct gaagaattta
      481 tccgcagccg aaaggcctac ggctcaagaa aaataaaaaa agccttatca aaacgaggca
      541 ttcagatcag ccgacgaaaa attagtagaa tcatgaaaaa tagaggatta aaatcgagct
      601 atactgttgc ttattttaaa gtacatcatt ctacttgcaa tgaagccaaa acgacaaacg
      661 tattgaatcg taaattctta agagacaacc cattagaagc gatcgtaaca gacttgactt
      721 atgtacgagt cgggaaaaaa tggaattatg tctgtttcat tttggatctg ttcaatcgag
      781 aaattctcgg ctattcttgt ggagaacata aagatgccgt tctagtaaaa aaagcattta
      841 gccgtatcaa acaacctctg acagaggttg agatttttca tactgatcgt ggaaaagagt
      901 ttgataacca agctattgat gaattattaa caacttttga catcaatcga tcattgagtc
      961 ataaaggctg tccttttgat aatgccgta
//
LOCUS       DANKKG010000311          976 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.319, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000311 DANKKG010000000
VERSION     DANKKG010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.319"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            164..748
                     /locus_tag="PW004_002944"
     CDS             164..748
                     /locus_tag="PW004_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2727833.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          315 a          140 c          193 g          328 t
ORIGIN      
        1 aaagtgttca taaaatcacc catttctgga cactttcttt ttttgcttta tttttcaaaa
       61 aaacaattca tttcatgttc ataaacccat aacaatatct atgttcagtt aaactatatg
      121 gataaagtta tgttacaata aaattaatga aggagtgatt tttttgaaaa taggatacgc
      181 ccgggtatct actggtttac aaaatttgga tttacaagaa gacagcctaa ataaatttgg
      241 ttgtgaaaaa atttttactg atcatatgag tggggcaaaa agtaatcgac caggattaga
      301 aatggccatt gattttgttc gctctggcga tacacttgtc gtttggcgct tagatcgttt
      361 aggtagaaat atggaagatt tgatttcaat cgtcaatcga ctgaatgagc gtggtgtgag
      421 ttttcatagt attcaagaaa atattacgat ggacaaatca agttctacag gacagctcat
      481 gtttcatttg tttgcggcat ttgcagaatt tgagcgtaat ttgatcctgg aacgatccgc
      541 agcaggacga gcagctgctc gtgcaagagg acggtttggt ggtcgtcctg aaaaattgtc
      601 tgaacaagat ttggaattac tgaagacttt agtggatagc ggtacaccta ttaagactat
      661 tgcggaacgg tggaacgtct caagaaccac catttatcgt tatttggcaa aaatggagga
      721 gaaaaatgga aagacaaatt ctcattagtt tttgtcattt aatgctgaaa atttttgcca
      781 tttatctaga aaagtgacaa tagtatgaga attttttgta agtgtctact gataacttat
      841 tctcaaatgt tgtaaggatt ttattcattt gttaatatta aagaacggaa ctccaaaaaa
      901 gtccgtactc taatttgcag tctctcactc ttttagagtg agagttttta ttagattttc
      961 aaattaatta gtaaag
//
LOCUS       DANKKG010000312          975 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.320, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000312 DANKKG010000000
VERSION     DANKKG010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..975
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.320"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            43..183
                     /locus_tag="PW004_002945"
     CDS             43..183
                     /locus_tag="PW004_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin RelE"
                     /protein_id="HDL2727834.1"
                     /translation="MYRVAYEIQENKVIPVLLYGTHNNFYELAENTRNFIRVDESASV
                     EL"
     gene            443..697
                     /locus_tag="PW004_002946"
     CDS             443..697
                     /locus_tag="PW004_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDL2727835.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDTIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            698..>975
                     /locus_tag="PW004_002947"
     CDS             698..>975
                     /locus_tag="PW004_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL2727836.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVG"
BASE COUNT          346 a          150 c          195 g          284 t
ORIGIN      
        1 ttatcaggta attggtctaa tggatttaag tatgcggtca ccgtgtatag agttgcttat
       61 gagattcaag aaaataaagt tattccagta ttgctatatg gaacacataa caatttttat
      121 gaattagccg agaacactcg caactttatt cgtgtagatg aatcggcttc ggttgagtta
      181 taactttagc tatatcaatc aaccgaccga catatgttaa atctatatat aataaaaata
      241 cctacttaaa tggatgttaa cggaagtgta tggtgagtag ttcactcgta ttttgatatc
      301 gtttgtatat gttttcagcg ttctaagtgg cttattttgt ccaataataa tatggagtgg
      361 aatcccttaa gaaacaccgt tggcaattgt cgttataaca tgttataatt tatttatcaa
      421 aaactaaaag aagtgtgata acatgggaaa agaactaaag gttagaaaga tcggcaatag
      481 cgtgggagtg atccttccaa gctctcttgg attaaaaagc ggagacacta tccaagccaa
      541 acaagaaggc aacttattca tattagatac tactcaaata gcaaaagaac acgatcgcaa
      601 attgattgaa gaaagtttcc aagactttga gaaaggttta acagtttcag aaattgaaat
      661 ggtaaaagct ttcggaaaat acggctggag tgaatagatg aaagagtaca cgctagttta
      721 ttctaaaagt ttccagacca gccttgaaac gataatagaa gaatgggaaa atgagcttgc
      781 tctttctcca gccagcataa aaagatttgt aggtgcgatc cagaaggctt tagaactaac
      841 caaaaatttt ccaactatgc atgaggaagt ttccgaaatc tatggaatgg atatcccaac
      901 atatcggatc gtgatcggca aaagctatgc cttattttat cggatcgacc atgaaaaaaa
      961 agagatactt gtagg
//
LOCUS       DANKKG010000313          958 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.321, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000313 DANKKG010000000
VERSION     DANKKG010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..958
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.321"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..210
                     /locus_tag="PW004_002948"
     CDS             <1..210
                     /locus_tag="PW004_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727837.1"
                     /translation="ITLSSGSAGYFMNDGGSKPQLTVRDRIAGFMHFSHYDLEEARKI
                     LAEYGITLINWEYAPPIRNFFNDYY"
     gene            397..>958
                     /locus_tag="PW004_002949"
     CDS             397..>958
                     /locus_tag="PW004_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HDL2727838.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
BASE COUNT          302 a          118 c          191 g          347 t
ORIGIN      
        1 atcactttaa gtagtggctc tgctggatat tttatgaatg atggtggaag caagccacag
       61 ctcacagtcc gtgatcgtat tgcaggtttt atgcattttt ctcactatga cttagaagaa
      121 gctcgtaaaa tacttgcaga atatgggatc acattaatca attgggaata tgcgccaccg
      181 ataagaaatt ttttcaatga ttattattag tcttataaaa aacagaatgt aaaatttatg
      241 tgcgcgagta tttagttttc cattttttgt ttgatagata tatagcattg ttgaagcatg
      301 ggatagtggt gccaatttta tcaatgaggt tggtcaggca gttaacataa ttggtgatgc
      361 tataaatcca tttaattgga attggaggaa ttaggtgtgg aaacagtgag attatggata
      421 ttcatgaaaa attttcgttc ggctttgaga tatgttattc tggaatcaaa atcaggttat
      481 tatctgattg acacagatca ctatactttt tggggaggct acctttttcc gatttttaat
      541 tggataatcc cccataaggt tatgaaaatt aacttgcctg cagaagaaat aaatagatta
      601 atactcaaca aagatttaca aaggaaaaga gagaaagaac attctaatgt tgcttggata
      661 ggtccagcat ttgcagtagt gacttcttca attgtcggtc ctatgctaga gagtatttat
      721 agtgattttt ctattggaat acgttattta tttgttactt taagtatagc tgttcttttg
      781 tgcataaagg gttatgttag caagaagaat aaagatattt taacactaat cggtgtaaac
      841 aatttatcta gtactaaaat tatactgttt cctgaatcta taaaagaagg atcaaggttg
      901 ttgataattt ttataatgct tatactgttt tctgttttat ttggggtgat gtttatag
//
LOCUS       DANKKG010000314          943 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.322, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000314 DANKKG010000000
VERSION     DANKKG010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.322"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..>943
                     /locus_tag="PW004_002950"
     rRNA            <1..>943
                     /locus_tag="PW004_002950"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DANKKG010000315          929 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.323, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000315 DANKKG010000000
VERSION     DANKKG010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.323"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(602..675)
                     /locus_tag="PW004_002951"
     tRNA            complement(602..675)
                     /locus_tag="PW004_002951"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(639..641),aa:Ile,seq:cat)
     gene            complement(865..>929)
                     /locus_tag="PW004_002952"
     CDS             complement(865..>929)
                     /locus_tag="PW004_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDL2727839.1"
                     /translation="TKGGAQDFHDKRWYLNIPKR"
BASE COUNT          311 a          157 c          163 g          298 t
ORIGIN      
        1 gactgacgtg ccggacgcac gtagcgatca tcggcgttat gactatataa agtatacttt
       61 cgttgatcca gaaaggtata aagattaaag taggctacct aaaaaggtag cccggaacga
      121 attttatcac catacttgtg aaaggagata ttttttaagt tagtattaag attgtgtaat
      181 atgatgatat ctatatttta tagtatcagt gctataaaat caaaaataag tcactaatta
      241 actaccactc caattgtaag ccttttttct cacttttttt caaaaatatg gtatgctttt
      301 taatggcttc aaatataaaa gagtttaaag cgtaacacac ttatggggaa gtggtttggg
      361 gtgcgcttta aactcttctt tattattatc tcacaattta acccaaatgt ctttctattt
      421 aaaaatcaaa gtaaaacttt tcaaatatac agaagtataa ctatgtgaaa catcctttca
      481 ttaatccata aaaggataca taaaaaagcc actcatttga gtggcaatga agaaaagctt
      541 tagcttgtat aatactcttc aaaaaaattc taacacagaa cgattcaaat ggctacgtta
      601 atgtaccctg taggactcga acctacgacc ggacggttat gagccgtctg ctctgaccaa
      661 ctgagctaag ggtactggtt gttgccacat aaagccataa acaatcaacc agtagaatgt
      721 gtggcaacaa acctgttatc gcatagcttg gagtgtgact atttatgggt gatagtgaag
      781 atatgcgata acatcactat tttatcgaat aatttttata gttgtcaata tagttatgta
      841 ctgctcctca acgaggagct atttttatcg tttaggaata tttaaatacc aacgtttgtc
      901 atggaaatct tgcgcaccgc ctttagtgt
//
LOCUS       DANKKG010000316          922 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.324, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000316 DANKKG010000000
VERSION     DANKKG010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.324"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            1..351
                     /locus_tag="PW004_002953"
     CDS             1..351
                     /locus_tag="PW004_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727840.1"
                     /translation="MSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSDKFRNYI
                     ADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELERIGTIG
                     IFEKNTSEEKNLKL"
     gene            507..881
                     /locus_tag="PW004_002954"
     CDS             507..881
                     /locus_tag="PW004_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HDL2727841.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPDVEWQHFF"
BASE COUNT          380 a           98 c          185 g          259 t
ORIGIN      
        1 atgtcaatta atgcaaatgg caaaaatgaa acttttaagc catcagatta tacattggag
       61 gcaaagaaag aatatgttta tgaatattta gggcttaaat ttaaactctc agacaaattt
      121 agaaattata tagctgataa aaagatagcg atgttagatg atcaaagtcc tatagataaa
      181 gaactaaagt atgctatttt gacatttgag aaaatgacag aagaacaaaa gaatgcagtt
      241 atagaaaaga tgggcgatgg atataaaaat tggcaaaatg agcttgaaag aattgggact
      301 attggtatat ttgaaaaaaa tacatcagaa gaaaaaaatc taaaattata aaatgtgata
      361 ctcatactaa aataggagtt tcaagtgatg gaaaatacga ttgctattta agcacaaata
      421 gcggagctga aagtaatctt ctggatgaat taaaaagaac tgagattcaa attatagata
      481 aaaaagaacg tcctaaaaat ggttttgtgc tatcagaaaa aactgattta gaaaatacag
      541 aagcatttaa caaagaatcg gttaaagatt tacgtaaact accaacaaag gacataaatg
      601 gcaaggattt tacaagtaaa gattttgaga agtatgatct tactatggta aatgtatttg
      661 caacttggtg tacagcttgt gtaaaggaaa ttcctgattt ggttgaggtt cagaatgaaa
      721 tgaaaagcaa gggtgtaaat atcgttggag ttgttacaga tcctgttgat gataacggtg
      781 aaaacaagga agcaattgaa aagtcaaagt taatacatga aaagacaaag gcttcatatc
      841 ctttcttaat gccagatgtg gaatggcaac actttttttg acaaaaatta taaggtgttg
      901 tgaatgctgc ggtgttatgg ct
//
LOCUS       DANKKG010000317          915 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.325, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000317 DANKKG010000000
VERSION     DANKKG010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..915
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.325"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(569..655)
                     /locus_tag="PW004_002955"
                     /pseudo
     CDS             complement(569..655)
                     /locus_tag="PW004_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          339 a          145 c          121 g          310 t
ORIGIN      
        1 tggatcaata gcaatttcat cacaaaccta tagaggatga gtgacttctg cctaattacg
       61 ttaaaaaata ttgaaagtaa ctctatgtag caaaaaatat ctagaatttc atttcctcaa
      121 aactgatcga tattgtatct aaaaatatat actacctaaa ataggtagta tatattaaaa
      181 tatgtgcttt tgttagaaaa atcaatacat aaattatcat atctgtatat gtcatttagg
      241 tatttcagtt atgttataat agttttgctg tctgctaccc cggcctaatc tggcaagggg
      301 aggaggagat ataatgaaat acctttttga ttttgttttt gcaatcaagc catttgcaga
      361 ggtaactgca cactacatct gcaagtggct tgattgcaaa attgccaaaa aagacagtaa
      421 gcctaccagt taattctata atatagaaac gtaaaaaaaa ccccatcagc ctacacgctg
      481 atggggtttg atgatataat gaaatacctc tttgttactc ataattatac cataattagt
      541 cacgaatgca accaatagaa aaaactactt agaacggtag atcatcgcta ataacaatgc
      601 attgtccacc aaatggattt tgtgaagcat taaatacatt gttattaatt gtcgctgttt
      661 ttaactaggc ctctcccctt ctattataaa agaagatata tattatttct taattaccta
      721 ctaccttaaa ccaaaattta agtactacct aatttaagta gtacttaaat aatctaaaaa
      781 atagttatcc caattgttat gcttttataa tacgattatt tgtaacaaaa tgaatattat
      841 ataattaaac actaccatat ttaggtagtg tttaattatt agcaaaacaa aaaatattta
      901 ttctattctc ttaac
//
LOCUS       DANKKG010000318          910 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.326, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000318 DANKKG010000000
VERSION     DANKKG010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.326"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..888
                     /locus_tag="PW004_002956"
     CDS             <1..888
                     /locus_tag="PW004_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HDL2727842.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT          389 a          120 c          149 g          252 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt caacttttgg
      901 ggttcagttc
//
LOCUS       DANKKG010000319          895 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.327, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000319 DANKKG010000000
VERSION     DANKKG010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.327"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            699..>895
                     /locus_tag="PW004_002957"
     CDS             699..>895
                     /locus_tag="PW004_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL2727843.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTL"
BASE COUNT          323 a          148 c          110 g          314 t
ORIGIN      
        1 aacacttttg tttctctctt tcctttctac atcattaaat agtattttat acgttatcta
       61 aaatactatt cctctgtcaa aataagctaa cttacttcat tataaaatat ccacttatcg
      121 ctttcatgta tattccaccg taaaaagtac attttatcac cgagaactca tcatttttca
      181 tcatgatgaa tactatttac atatatctag ttcaaagaca ataattatta tgatccgtta
      241 ttatctaagc actatacaga taataggaat ttttcgtttc taaatactcc aatttgggaa
      301 tccaataaaa attttcgttc aaatacgtgt ctattcgaac tcttttctag ttgttaatta
      361 tgaaggggga agaaataaaa aatacaatct atacacgaat aagaatatta tccaaaagga
      421 ggttttagaa aagatttatt atacgtaaaa agctcaagac attttcttag ccataaggac
      481 atgtttgata tttaagaaga tatatgaatt ttcttttcta agtataaaga taactatcca
      541 ttatcaacta aaaaaacatt tttgaaaata aacatcagca gtcctatcaa tgcatgtgag
      601 cttatagaag ttattcgcag cgcagcataa ttgcattagc ttcttttcgt gttttttgcc
      661 tcttttccct ttaaatctta atggaggagg tgtgcaatat gtcagataac gacgaacgcg
      721 taaatgccca gttgattcga ttctttgaaa aggtcatttt gaacaccgct aatacttatt
      781 ataaaaaaca agaagaaatt caaaaacatg aacaacttga tcaactccca ccatatttgt
      841 ttactaaaga tactataaaa tttaaacaac cgattaatgt tttaaatgtg actct
//
LOCUS       DANKKG010000320          890 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.328, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000320 DANKKG010000000
VERSION     DANKKG010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.328"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            27..719
                     /locus_tag="PW004_002958"
     CDS             27..719
                     /locus_tag="PW004_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDL2727844.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          396 a          139 c          149 g          206 t
ORIGIN      
        1 aagtattgga ttaaggagct atgcaaatga aaaagaaatt tattactggt gggattgtcg
       61 ttgcatttct atgtctaatt ggctacacag gcacgcggta cgcaaatgaa attattttgt
      121 ggggtggaga gacagatatt gatacaatca atgagaatct gacacagctt gatcatacat
      181 tgacagataa agagcaagcg attacagatc ttaccgctcg actagcacaa aaagaacaga
      241 cattgcaaga ttcaaaggca gatgtccaag aataccaaac aaaagtaaat gaattagaaa
      301 accaaaaaaa tcaatcggca gcagaaaaac aaaacttaga aagtcaatta gtcagcaagg
      361 atgcacaact acaaagcaaa caaaatgaaa tcaatgtcaa actagatgag atcaaccaaa
      421 aaaatgatga aatcaatcat ctgaatgcag attggacagc aagactacaa gaagcacaag
      481 caaaactttc agaattacaa aatactgtaa atggtttgaa tcaacaaatc gcaacactga
      541 cacaagaaaa aacaaatgta gaaactcagt taacagatac acaaaagaaa ttagacgaag
      601 caactgctga aaactcaagc ttgaaacaat atattcaaaa gttagaaaaa gcaaaagatg
      661 aagtggaaga tacagctgat aaatctcagc aaattgttga agaacatttg cgtaaataat
      721 ctttcttaac aagtaaacct aaattagtaa agaagcaaca gtaatctata ctaagtagtt
      781 cagttttgga gtacaaaatg acaaaatgta cttcaaaatg gactactttt ttcattttaa
      841 cttacatacc aaattacata caaaatgatt tataaaatgc aattgaaaat
//
LOCUS       DANKKG010000321          887 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.329, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000321 DANKKG010000000
VERSION     DANKKG010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..887
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.329"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(223..296)
                     /locus_tag="PW004_002959"
     tRNA            complement(223..296)
                     /locus_tag="PW004_002959"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(260..262),aa:Ile,seq:cat)
BASE COUNT          325 a          144 c          135 g          283 t
ORIGIN      
        1 tttattatta tctcacaata taacccaaat gtctttccat ttaaaaatca aagtaaaact
       61 tttcaaatat acagaagtat aactatgtga aacatccttt cattaatcca taaaaggata
      121 cataaaaaag ccactcattt gagtggcaat gaagaaaagc tttagcttgt ataatactct
      181 tcaaaaaatt ctaacacaga acgattcaat tggctacatt aatgtaccct gtaggactcg
      241 aacctacgac cggacggtta tgagccgtct gctctgacca actgagctaa gggtagtggt
      301 tgttgccaca taaagccata aacaatcaac cagtagaatg cgtggcaaca aacctgttat
      361 cgcatatctt ggagtgtgac tatttatgag tgatagtgaa gatatgcgtc tccatatatg
      421 tatggttgag acagataaac atgtatgaaa aacgttgtta aatcaacgtt tttttatttt
      481 ggtcaaggag agaaaagtag agaaatacaa aattttttgc attgattttg cattcaaaaa
      541 aacattcaaa catttagaaa tccccaatac gtattcagga aaatctattt taaggtttac
      601 aaaaagaact tttgttcgta cactcttttt gaggtgaact tttatgctga tggaaggaaa
      661 aacacaatta tggtttaagc atgttccttc aaataagttt tttaattata aactctagag
      721 aaattaattc atcctaacat aaaataattg ttcactcaaa agtttttcta gaacaattaa
      781 aacagcaggc ttcacattcg ctatacattt gtaagaagta ataattcctg tactaaaagg
      841 cataattgct ttaattattt tttgacttct cttcaaacac aagaagt
//
LOCUS       DANKKG010000322          884 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.330, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000322 DANKKG010000000
VERSION     DANKKG010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..884
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.330"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..159
                     /locus_tag="PW004_002960"
     CDS             <1..159
                     /locus_tag="PW004_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDL2727845.1"
                     /translation="RRNKFYRSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVSTEI
                     KVLMGIPA"
     gene            242..859
                     /locus_tag="PW004_002961"
     CDS             242..859
                     /locus_tag="PW004_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727846.1"
                     /translation="MWIFKQEIFAKLFSLDGLGWGILFFIFLFLGSMWIWFVDKKNSY
                     EKVIVKKMNRNEFDDFSKEHRVELYDSHEINSTSPSYYWVFGCEEKPLEIKLLKMLEN
                     PPVIEDFSAKNVEKQMFEQVQIRGQNKISLEPDEYAAFFLETNSGTSMPVFKVKLKMN
                     GRLCTYDLVANMRYGSTGDRRTILKFEQTFRSFLKNRVSQIFNLD"
BASE COUNT          310 a          109 c          172 g          293 t
ORIGIN      
        1 cgacggaata aattttatcg aagtctccgg actgcctcat ccacgattaa ggggatggag
       61 accattcgag gaatatataa aaagaaccga agaaatggaa cgctcttcgg cttttcggtg
      121 tctactgaga tcaaggtatt aatgggaata ccagcctaag atatttggag ttcagagagg
      181 gcgcgtttga ttttcaaact tcgcaacaga accattattt taaagtggga ggaaaattgt
      241 tttgtggata tttaaacaag aaatctttgc taaattattt agtttagatg gattaggttg
      301 gggaatctta ttttttattt ttttattttt aggatctatg tggatctggt ttgttgataa
      361 aaaaaattct tatgagaaag taatagtgaa aaaaatgaat cgaaatgaat tcgatgattt
      421 ttcaaaggaa catagagttg aattgtacga tagtcatgaa attaacagta cttctccatc
      481 ttattattgg gtatttggtt gcgaagaaaa accattggag attaaattgt tgaaaatgct
      541 tgaaaatcct ccagtcattg aagatttttc tgcaaaaaac gttgaaaaac agatgtttga
      601 acaggtacaa ataagaggtc aaaacaaaat ttcattagaa ccagatgaat atgcagcctt
      661 ttttttagaa acaaattcag gtacttcaat gcctgttttc aaagttaaat taaaaatgaa
      721 tggtcgttta tgcacttatg atcttgttgc aaatatgcgc tatggttcta ctggtgatag
      781 acgtactatt ttaaaatttg agcaaacctt tagaagtttt ttgaaaaata gagtatccca
      841 aatttttaat ttggattgaa aaaattaaaa atttggccaa aaat
//
LOCUS       DANKKG010000323          859 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.331, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000323 DANKKG010000000
VERSION     DANKKG010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.331"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
BASE COUNT          301 a          141 c          133 g          284 t
ORIGIN      
        1 aaaatacgaa tatatttctt ttgtagtaaa atcggactgg tttgcatatc attttcttcg
       61 taaatatcaa aatcatgtta actactatca attactaata aaaactacca ctttaagcgt
      121 taagaactgt taagtttagt taagtgagga tagttcacaa aatccattgt taaacttcac
      181 gaaagtggta aaactcaaaa tgctttatat aggattacag catcgctctt tcttcgattg
      241 cgcgttgggt aaaaaatatt atgaagtaaa aattgacgat gacacgttcc ttacggctca
      301 tcagatgaaa cagctacaaa aacgaaatga tcaactcgaa gaaaatttaa ttctaaaaaa
      361 gcgattgcca tattcacgcc tcgctcgacg aactattaaa ggtcaagatt tacctgaaga
      421 tcatcctttt taggaaattg aagcttttta tgttgcacaa tgcatttata atactacaat
      481 gatgttttca ccagatattc ttctagggtg cggtgtaatg aaacagatac acttgcaaaa
      541 gaaaattcac cgcgaattta ctaaaattat gaataattat gttaatcttc cgaaattttt
      601 ttccaaaatt aagggatcaa gctggtgtta tagattgttt aaggttggca aaaaaataac
      661 ttctttttaa acaattatag aaaaccgata ctctgttaag ttggttgaca cggtgtcgat
      721 ttttatgact ttcttattct gaaacttcac taacggacag tacctaaaaa gtctatagta
      781 tggacgttta cgagtactat tttatcaaaa atgctgctca tttcaactta tatgttttag
      841 tgaactaccc ccacttagc
//
LOCUS       DANKKG010000324          845 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.332, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000324 DANKKG010000000
VERSION     DANKKG010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..845
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.332"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            228..>845
                     /locus_tag="PW004_002962"
     CDS             228..>845
                     /locus_tag="PW004_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727847.1"
                     /translation="MYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILP
                     LILLGFIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEI
                     FLIIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSID
                     SELLSFGIGLTLLICLIILTFNFKKLGILSKITYIMGIISILLCTK"
BASE COUNT          271 a          118 c          119 g          337 t
ORIGIN      
        1 atgatacggc atttcacatt aatcgtgcgt taagtttaga atctattttt agctccccta
       61 ttaattttga aacgtttaga tcatatggca tgcaagtaaa taatttttac ccatggttaa
      121 ctctatatcc tttattttta ttgatcaagt ttactaattt agcaataggc tataaccttt
      181 ttctttatat tgttacgctc attactctat ttatatgtca ttatgtaatg tatgaaatta
      241 ccaaaaaaca tgttactagc tcctttttcg ctattatata tacaacatct tcttttagga
      301 gtgtagaaat atttcttcga ggggcaatgg gggaattact cgcaatgtca atacttcctc
      361 ttattttact cgggtttata aagctttacg actcaaaaaa agaatcttgg gttatgttgg
      421 cgattagcat gactttatta atttacactc atgttctttc tgtagctatg actatggtaa
      481 tgattataat tgctttaatt attcaatttt atcggaaaaa gaaaatagaa atcttcttaa
      541 ttattaaatt aataaaagct gctatagtta ctttacttct ttcactagcg tttataggtc
      601 ctatgattga gcagacattg tatcaagatc tgaatagacc ttatgtggat atattacaaa
      661 aaagagcaat ttatctgggt aaagagtttt tgattggaag tatagattca gaattactat
      721 catttggtat tggcttaact ttgcttattt gtttaattat actaacattt aactttaaaa
      781 aattaggaat cttatctaaa atcacttata taatgggtat aatatctatt ctactttgta
      841 caaaa
//
LOCUS       DANKKG010000325          836 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.333, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000325 DANKKG010000000
VERSION     DANKKG010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..836
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.333"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(61..336)
                     /locus_tag="PW004_002963"
     CDS             complement(61..336)
                     /locus_tag="PW004_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2727848.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            568..>836
                     /locus_tag="PW004_002964"
     CDS             568..>836
                     /locus_tag="PW004_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2727849.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILG"
BASE COUNT          302 a          138 c          120 g          276 t
ORIGIN      
        1 aacatacaga taataaaaac aaactcttta ttactatatg ttttagtctt atttgtttac
       61 tcaaccttta taccgttcca tgaaactatc aatatcagat tttttaaatc taatgaccgt
      121 atcaattttt ataacaggta acccgtattg agaaacatat tttctaatcg ttcctactga
      181 tacacctaga tattcacttg cctgctgata gtccatatac tctagagtga ctccagaaat
      241 aatgtcttct agcataggct ttagcactgt aattatctct ttcacttgtt gttcaacttg
      301 tatagaaaca atgctatcaa tgatttgtag agacatattt ccccctcctt tcgtgattgc
      361 ttgaacaaat tttctaagaa ttatcttcgt attttttgct caaaataaaa gtattttgtt
      421 attaaccgat taatcaaaca aaaaatgatt accagttcat cgacatacac ataatacctc
      481 gataaaaaag aggtgtcaat atctttgtaa aatttattta caacacccct ttttcgaggt
      541 atactaattt taggaggtgt ggtcaatatg ataaaacgtc caccaataaa ttatttagaa
      601 cgaaaaaaga tactcggaac aaaaataaaa gctataagaa agagtaaaaa attaacacaa
      661 ccagcatttg gtcttatgat taacaacggt caattaattg acaaaaaaac tatctacgaa
      721 tgggagaagg gaacctattt accaatacca gagcgattat cccgaattgc tgatttggga
      781 aatatgagta tagaggagtt agtttgtggc aatgtagaag aatatatttt gggaat
//
LOCUS       DANKKG010000326          836 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.334, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000326 DANKKG010000000
VERSION     DANKKG010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..836
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.334"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..>836
                     /locus_tag="PW004_002965"
     CDS             <1..>836
                     /locus_tag="PW004_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HDL2727850.1"
                     /translation="STFLNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRI
                     MIKGAHFFDKKQWRYALKISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISN
                     IGIILSVIWMSTNNAWVPWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLA
                     KFMAPKDYYSGLSLVVPITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILL
                     NMIFIPRLGIIAAGYTTVFTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFVSIFSL
                     IL"
BASE COUNT          269 a          105 c          141 g          321 t
ORIGIN      
        1 tatctacttt tttgaatatt gcaatctctg tagctttgat gcagacatta tattttgatg
       61 ataaatatgt tggcagagta gtcggcggaa cactgtcatt tgtgttgata ggtttagtaa
      121 tttatttaag aataatgata aagggtgctc atttttttga taaaaaacag tggagatacg
      181 ctttaaaaat ctctttgccg ctaatcccac attccttagc aaatatagtt ttagcacaat
      241 tcgatagaat tatgattaat tcttatttag gtcctgctgc ggggggaata tattcctaca
      301 ttagtaacat aggtattatt ctaagcgtaa tatggatgtc tactaataac gcctgggttc
      361 catggttcta tggtgagatg agtaaaaata atgaaaaaaa agtgaagaaa gttagtaact
      421 attatttagt agtctttact ttgattacga tgattgtaat ggtagtttcg attgatttag
      481 caaaatttat ggctccgaaa gactattatt ctgggttatc tttagtggtt ccgataacct
      541 taggctattt ctttcagttt ttatatagtt taccagttaa tgctgaattt tatgagaaaa
      601 aaacatcgtt tattgcgatt ggtacagtag caagtgctat agttaatata ctattaaaca
      661 tgattttcat acctaggtta ggaataattg ctgctggata cacaaccgta tttacgtatt
      721 ttttactttt tatttttcat tataatatcg ctaaaaaaat tacgaataaa caattatttg
      781 acacaaaaaa aatatgtatc ataataattt ttgtatcaat tttttctttg atactt
//
LOCUS       DANKKG010000327          833 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.335, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000327 DANKKG010000000
VERSION     DANKKG010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..833
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.335"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            28..699
                     /locus_tag="PW004_002966"
     CDS             28..699
                     /locus_tag="PW004_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2727851.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
BASE COUNT          325 a          124 c          125 g          259 t
ORIGIN      
        1 taaaataaaa gttaaggagc attgtgtatg acaattaaaa ctagtctcca aaatcaagct
       61 taccaatcta ttcgccgaca aattatctat gctgacttag aacctggaaa aaaagtatca
      121 gaaaaaaaat tagaagaaac actttctatt ggtcgtacac caatacgtga agcactgatt
      181 caactccgac aacaaggtct tgttgataca attcctcaaa gcggaaatta tatctcatta
      241 attgatttag attcggccaa aaatgctcga tttgtcagag aacatttgga acgacaaatt
      301 atgttagagt gttgtgccaa aatgaataac caaaaaaaac ggattttaga aactataatt
      361 tcagaacaag aaaaagctgt tagtcaaaaa aatgctcgta gtttttttca taatgataat
      421 ttattccata aagcttgttt tgaaattgca gagcgagaag aagtatggca atggttagaa
      481 aataacaata cacacttaga acgatttcga tggctacgag taaaaacgga aggattaaag
      541 tgggaaaaaa ttatggaaca acattatcaa ctatttgagg cattaacaaa acaagatcca
      601 gaagaagcta actttctaac atcgttgcat cttcatttaa tgcttaatga gcaaaatatt
      661 gttttagcta ctcatccaaa atatttcaaa aatatataac ttttaactct ttttaaatta
      721 aatggttgtt ctgatcagaa aaatatcgct tgttttctta ttatgtaata tatttcataa
      781 actattctgc ataacaacta ttgtttgtac tttttacatt ggataactaa tta
//
LOCUS       DANKKG010000328          809 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.336, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000328 DANKKG010000000
VERSION     DANKKG010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.336"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(194..742)
                     /gene="aac(6')"
                     /locus_tag="PW004_002967"
     CDS             complement(194..742)
                     /gene="aac(6')"
                     /locus_tag="PW004_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HDL2727852.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          216 a          181 c          127 g          285 t
ORIGIN      
        1 tgtagaaagc cattttcaca aaatttttct tactactcga aactaagcga ctttttcaca
       61 acctctggta cacggtgttc caaaagtagc ttcttcggaa ataagttgga atttcacaaa
      121 aattcaagaa gtaattttcg taaattcctt cttatttctt gaagctgatt acttctgtca
      181 caacctcttt ttattattga gaatttggtc gaggaataat cgtttttgcc atccaaatat
      241 ccggtttgtc ccagccattt gcatttggta agacacctac gattttataa cctaattttt
      301 catagaattc atacggatgt tcacgaaggt tctggatgga agccacttta tcaaatgtat
      361 gctcatacag gtccgtttga cttaacgttg ttccatggtc taaatcatcc gtacctaaat
      421 aaatcgtgat tcctcctctg gaagctactt ctttttctaa gtaattgact aatcgagtac
      481 ctatttggtt ctttcgtcgg gagctttcta caactaatgg atgcaattcc caacctgtga
      541 taccgtattg agggattgca ccaataaatc ctactaactc atcttggtct accgctgcta
      601 ccgcgattcg ttctggattc atcatttctt ctacttcttc tgccgagctg tctccatatt
      661 cttccggcca agtcagtctc agtaaatcag aaagctgatc tttcaatact ggattattac
      721 ggtcaaattc actgattatc atacgtcttc ccccttgttc ttgcttattc taacagtcct
      781 tttgaaaaaa cgaaatgttc tcgctcaaa
//
LOCUS       DANKKG010000329          789 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.337, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000329 DANKKG010000000
VERSION     DANKKG010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.337"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(314..514)
                     /locus_tag="PW004_002968"
     CDS             complement(314..514)
                     /locus_tag="PW004_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2727853.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
BASE COUNT          287 a          123 c          126 g          253 t
ORIGIN      
        1 tgctttgctc ataaaaatct gcatattcat cactaatatt ttttctaata aattcttcca
       61 tcataaagat cacctcgaaa agagtatacg aacaaacgtt ctttttgtaa aggtggaatt
      121 tattatacta aataaatagg tgaacaagta cttgtgccaa attatgtgcc aaaaaaaatc
      181 gaatttaata aaacacaaac aaaaagaaat cctattatga taacatttct tataataaca
      241 aacacaacag aagacgtgta atagttagtc aggaacgtac aaataacccc tgtatccttt
      301 gcggtacagg gggttatttt gtataagtag acattgtagt ttctaaactg ctatgtccta
      361 aacgtttcga tacgctaagt atatttactc cttgataaag taaaatagat gcatgcgtat
      421 gtcttagtcc atcaataaac agtcgaagag aaaataggga aaaattgatt actaaagaaa
      481 aacttactag gtcgacggtg tagttgattt tcatacttca atagattcga attaaacgat
      541 ttcgattggc ttataccagt cctatccaac gaaagatata tcgttcttaa ctctagcggt
      601 ataacttgaa gctttacgta aagccaatgg gaatgaactg gagatgactt tttattattt
      661 ttagtaatag ctgatgggtt aatacttaca actttccttt tcaaagtacc atataaaaaa
      721 acggtggggt tgtttagcga aataaaggat ctaagccaat agcttagatc ctttattcta
      781 tgctttata
//
LOCUS       DANKKG010000330          783 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.339, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000330 DANKKG010000000
VERSION     DANKKG010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.339"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            24..716
                     /locus_tag="PW004_002969"
     CDS             24..716
                     /locus_tag="PW004_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HDL2727854.1"
                     /translation="MGLAFLLAGCTNGNESKKESTKETAKATVATTTTTSTESKSTAA
                     STETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNADKSAQLEAF
                     MTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEWSESGTGQA
                     DYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNETANNELK
                     NGFNQIVYGQAN"
BASE COUNT          281 a          151 c          159 g          192 t
ORIGIN      
        1 tgaaaaaaag gattttagta acaattggtc tagctttttt gttagctggt tgtaccaacg
       61 ggaatgaatc gaaaaaagaa tcaacaaaag aaacagcgaa agcaacagtt gcaactacta
      121 caacaactag cacagagagc aagtcaactg cagcaagtac agaaacagca gatacctcta
      181 cttcgtcaga gtcttccaaa acgactgaaa caactcaaac atctacttca agttcttctg
      241 tagaggcttc atcttcctct tcagaaagca gcgaacaagt tgaacaaaca ctgtggaatg
      301 cagacaaatc cgcacaatta gaggcattta tgaccaaatg gggccaaaca atgggacaac
      361 aatacaaaag ttatacgaat caaatgagtg tggacttata tggtcttaaa gtccctcaag
      421 ctattttgaa cggggagtgg aagatggcta ttggtggagt gcctgtttct gcagaatggt
      481 cagaaagtgg gacaggtcaa gcagactatc aaatcacagc cgtctattcc gatgcagaga
      541 cagaaccata tctgaaaaag cacgtctatc tattcgggtt tcagcaaaat cagccaaaag
      601 tattggtgac acaacaaaat caggggaatc ccgataatta tctttacttc aacgagacag
      661 cgaataatga attgaaaaat ggctttaatc aaatcgttta tggacaagca aattaatgag
      721 ataaacaagg ggttgtgaca tgtcttttat cacaacccct tgctcagttt atttgttagt
      781 ctt
//
LOCUS       DANKKG010000331          764 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.340, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000331 DANKKG010000000
VERSION     DANKKG010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..764
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.340"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            659..>764
                     /locus_tag="PW004_002970"
     rRNA            659..>764
                     /locus_tag="PW004_002970"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          314 a          124 c          134 g          192 t
ORIGIN      
        1 attccttagg cagcttttgc tgtgtagaaa ggaataaata ataacgaaag ttcaaaagct
       61 aatgccgact attctagaca ttaaagaaga cgaaaacgaa cgataaagaa agaaaagtta
      121 tgaataatcg tttattgaaa aaaacttgaa aaaatacaaa aaattcgttg accaagctaa
      181 atttagttgg tatattatta cttgtccaaa agacagaaga aaatatttat tcaaaaacat
      241 aacaaaaaag ttattgacat gttactaacg acatgataag ataactgagt cgttaaaaaa
      301 gaaccacttg cgaagcttga ttctatcgaa aaaaacttca aaaaagttct tgacaaataa
      361 caaacgataa gttatgataa atgagttgcc gaaagatttc gaaaaacgaa atcgaaaaaa
      421 acttttaaaa aagagttgac aatcgcttgt ctctctgata tgatataaaa gttgctacgg
      481 cagccaaaaa tgaatcggga aacactctcg atgaagtaga cctttgaaaa ctgaacaaag
      541 taagacaaac caaatgtgta gggcgtcttg attcaattca agacaacaaa catttttaac
      601 aagcaagcaa tatgctagca aacaaattga gcttaacaat cgcaagattg ttcgaacttt
      661 ttatgagagt ttgatcctgg ctcaggacga acgctggcgg cgtgcctaat acatgcaagt
      721 cgaacgcttc tttttccacc ggagcttgct ccaccggaaa aaga
//
LOCUS       DANKKG010000332          755 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.341, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000332 DANKKG010000000
VERSION     DANKKG010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.341"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(75..674)
                     /locus_tag="PW004_002971"
     CDS             complement(75..674)
                     /locus_tag="PW004_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HDL2727855.1"
                     /translation="MNKKYSAGLVSKRFWFSEFKLYIQLRLEGYTDIEIKEKNEHENI
                     FFAVSSNRQKEIYQNVKRRVSQLDEAGLQLFNQLNIDNQKLVNLISILMIDDLFNEFM
                     TEVYAENLRKGNLQLTPVDYRSFFTEKRRSSEDISKWKDYTIKRLIAAYQTNLIETGL
                     VRGEKEYGRMTPILLDKRLIQWLVDKNRKDIVLALGGIL"
BASE COUNT          236 a          144 c           86 g          289 t
ORIGIN      
        1 acgattcaat aaaaaatctt ctttttgaat tacttcattc aacttttcca aatgctcttg
       61 aatcgataat gacattataa aattcctccc agtgctagta caatatcttt acgattcttg
      121 tcaactaacc attgaatcaa tcgtttatct aataatatcg gagtcattcg tccatattct
      181 ttttcccctc tgactaatcc tgtttcaatt agattcgttt gataagcggc aataagccgt
      241 ttaatcgtat aatctttcca ttttgaaata tcttctgaac ttctccgctt ctctgtaaaa
      301 aaagaacggt aatcaactgg tgttaattgc aaatttcctt tacgtagatt ttcagcatat
      361 acctcagtca taaattcatt aaaaagatca tcaatcataa ggatggaaat caaattcact
      421 aacttttgat tatctatatt taactgattg aaaagttgca atccagcttc atccagttga
      481 ctaactctcc ttttcacgtt ctgatagatt tctttttgac ggttactgct cactgcaaaa
      541 aaaatatttt catgttcatt tttctcttta atttctatat ctgtatatcc ttctagtcgt
      601 aattgaatat ataacttgaa ctctgaaaac caaaagcgtt tagaaactag accagcagaa
      661 tattttttat tcaatagatt gcctcctaat catctataat tcaataagta aacaagtgtt
      721 ttttgctcat cgttagcatt cattttttag tatct
//
LOCUS       DANKKG010000333          750 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.342, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000333 DANKKG010000000
VERSION     DANKKG010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.342"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            102..>750
                     /locus_tag="PW004_002972"
     CDS             102..>750
                     /locus_tag="PW004_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727856.1"
                     /translation="MEFWNSKGSIGTIGTTDSVGNPFPGASTPTPIEDNSLVIRTNGD
                     GKYILISPKVGKGLVLLGNGKAIYFGDLDVQGKLTVKGKEIIPGQGGGPSGGGESTGG
                     YPSELKTDAEKRAWRIYDILCNNGFTKQSACGILGNIQQETGGTFDPDTVQIGGPAYG
                     LVQWDGSSYPLVGPATWDGKVYVQNLFNAAGIKEPITSLDAQVRLLIWTFTNGQWM"
BASE COUNT          261 a           99 c          168 g          222 t
ORIGIN      
        1 aaaattcttt taacaagact ggagatgtat tgaaattagt taatggacta ctccaaattt
       61 ggaacaacaa aaagaaaata atggagttaa ctaaaaaagg aatggaattt tggaattcta
      121 agggttcaat tggaacgatt ggaacaactg attctgttgg taatcctttt cctggggctt
      181 ctactccaac ccctattgaa gataattctt tagttattcg tacaaatgga gacggcaaat
      241 atattttgat ttctcctaaa gtaggaaaag gattagtttt attaggaaat ggtaaagcaa
      301 tttattttgg agacttagat gtacaaggaa aacttacagt taaaggaaaa gaaataattc
      361 ctggacaagg tggtggtcca tcgggaggtg gagaatctac tggtgggtat cctagtgaat
      421 taaaaacaga tgcagaaaag agagcttgga gaatttacga tattttgtgt aataacggat
      481 ttacgaaaca atctgcatgt ggaatattgg ggaacattca acaagaaaca ggaggaactt
      541 ttgatcctga tactgttcaa ataggtggac cagcatacgg attagttcag tgggatggtt
      601 cttcatatcc tcttgttggt ccagcaacat gggatggaaa agtttatgtt caaaacttat
      661 ttaatgctgc aggtattaaa gaaccaataa cgagcttaga tgcacaagtt cgattgctta
      721 tttggacatt tacaaatgga caatggatgg
//
LOCUS       DANKKG010000334          746 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.343, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000334 DANKKG010000000
VERSION     DANKKG010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.343"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            191..>746
                     /locus_tag="PW004_002973"
     CDS             191..>746
                     /locus_tag="PW004_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2727857.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLIKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNA"
BASE COUNT          303 a          127 c           90 g          226 t
ORIGIN      
        1 ttaaaaacaa gaatatcttg acaaaatacc ctaggaaata aacaatagaa taaacaaggt
       61 aacaaaaaaa ttaaataacg ttatataaaa aagaaggcga cactctgtga gaataacaca
      121 gactgaaatg actttccact cacagttttt gtctaactat atacaaaaac agaataaaaa
      181 ggaggtattt atgctaattg aagcaagaaa aaaagagtta cttgttctac aaatggtcat
      241 tcaaaaaaat aattgttctt tacaagaatt atccaatgac ttacatattc caaaacggac
      301 aatcaaagaa ctgatccgaa aaataaatgt atcactcaag caatttttat tgattgatga
      361 atttatttat tctaatcata aaggagagat ccaaaccaaa gaaaccgatc aaactaaaaa
      421 gttagctgtt ttttctaagt taaaattatg ctacctaaaa gaatccaatc gttttaacta
      481 tttgatgtta ctaatcaatt atccacaaac tgctgtacca aaaaaatatt tacttgaaca
      541 actttatata tcgccctcct atctcgaaaa actgacaaaa cagctcaatc attccttaat
      601 aaaattccat ttacggatcc tatctgtaaa aaattgctgt actcttgaag gaaatgagtt
      661 atctatccgt ctctatcttt acttttttct ttcagataca tttggaggta acgattggcc
      721 attttcctca cttgaattaa atgcac
//
LOCUS       DANKKG010000335          742 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.344, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000335 DANKKG010000000
VERSION     DANKKG010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.344"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(<1..>742)
                     /locus_tag="PW004_002974"
     CDS             complement(<1..>742)
                     /locus_tag="PW004_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727858.1"
                     /translation="MKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLP
                     LILMGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIA
                     RLLSLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYN
                     NYTIGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQ
                     FVWRLNGFSSLFIAYTMSIVIYYIFSTKN"
BASE COUNT          305 a          116 c          117 g          204 t
ORIGIN      
        1 tatttttagt agaaaaaata taatagatca ctatactcat cgtataggct ataaataaag
       61 aagaaaaacc atttaaccgc cagacaaatt gtagactctt aactggtgta tggttaaaaa
      121 gttgccaagg aaataaattg gttgaacaaa aaagaacaaa tacaccaaga aagaaaataa
      181 ataaatcatc agcagttaat ttttttataa aaataaaagc accaatcaat ccgagaaata
      241 agaataatcc aatagtatag ttgttatagt tattatttaa aatgtgtgtc agcatatcac
      301 taggagccat accacttaat tcttttccca gtgggacttt caattcttgt gctctagatt
      361 gttgaataaa aggaattaag aatcccgcac ttaaaaaaaa ggtgaggacg gtagctttta
      421 aaagacttaa gagtctagcg attttttggt cccaaaaata aaaagaaaga aacaacgttc
      481 ctccaataaa tacagaaacc atagcgacag aaagtaaatg agtatacact actagcgtca
      541 tgccaataga caaccagtac catttttggt aatctctaat ataaatttcg taacacccca
      601 ttagtatcaa aggcaaaaat gttaaagcga cagcttctcc caatgaagca cgatggaata
      661 tgtcaatggc acgataggcg gaaaatgtat aaataatcga aaataacagc gaaatataac
      721 gattgttttt tatttgtttc at
//
LOCUS       DANKKG010000336          735 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.345, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000336 DANKKG010000000
VERSION     DANKKG010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.345"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..450
                     /locus_tag="PW004_002975"
     CDS             <1..450
                     /locus_tag="PW004_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-3-methylglutaryl-CoA lyase"
                     /protein_id="HDL2727859.1"
                     /translation="EEVVIISVNFEYSHIDIDYVFLSNLRRFHELPKESRTKCIVTSN
                     IQASDVYLRTSYKKLLSEREVVRDNAGLMAIRFLADMNVDEIFLAGFDGYSHNEDENY
                     GEQSMEIITKFALLDAMNVQMRDELSELAKKVKVTFLTDPRWVRIDK"
BASE COUNT          272 a           68 c          150 g          245 t
ORIGIN      
        1 gaagaagtcg taataattag tgtaaatttt gaatattctc atatagatat agattacgta
       61 tttttaagta atttaagacg ttttcatgaa ttaccgaaag aaagcagaac taaatgtatt
      121 gttacatcaa atatacaagc atcggatgta tatttacgga cgagttataa aaagttgtta
      181 tctgaaaggg aagttgtaag agacaatgca ggattaatgg caattagatt tttagccgat
      241 atgaatgtgg atgaaatatt cttagctgga tttgatggat attcacataa tgaagatgag
      301 aattatggtg agcaatccat ggaaattata acaaaatttg cattattaga tgcaatgaac
      361 gtgcaaatga gagatgaatt gtcagaatta gctaaaaaag taaaggtgac atttttaacg
      421 gatccacgtt gggttcgaat tgataagtag tagtaataat gtttttaaaa ttttgataaa
      481 gagatagtga gagaataaaa atgggtaaag tcttaaataa aaaagaagaa aaatatttaa
      541 gggccgatgg ggcgagatga aactctaatt ttgaactttt aagaatcatt tctatattgt
      601 gtattgttgc ttaccatgct aatttgcgtt taaattataa tgcagacgga attatagtac
      661 atggttcatt aactgctaac cagattttct tgaattatat tggtatttgg ggtcagctag
      721 gagttaattg tttta
//
LOCUS       DANKKG010000337          728 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.346, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000337 DANKKG010000000
VERSION     DANKKG010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..728
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.346"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            23..646
                     /locus_tag="PW004_002976"
     CDS             23..646
                     /locus_tag="PW004_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727860.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
BASE COUNT          252 a          113 c          156 g          207 t
ORIGIN      
        1 aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca
       61 agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg
      121 tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga
      181 aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt
      241 gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt
      301 gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc
      361 tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca
      421 tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg
      481 aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga
      541 gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt
      601 ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat
      661 gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc
      721 aaaataac
//
LOCUS       DANKKG010000338          723 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.347, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000338 DANKKG010000000
VERSION     DANKKG010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..723
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.347"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..>723
                     /locus_tag="PW004_002977"
     CDS             <1..>723
                     /locus_tag="PW004_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL2727861.1"
                     /translation="VYLFFKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKV
                     VQGWTPDVIVGRAAFPISCSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTF
                     RRSIHEREKDYSQFSNEFGHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAID
                     IEKKLNQWFESVPKNLFKSITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNEN
                     SNGLLRRDGLLKSMDFNSVDEF"
BASE COUNT          245 a          121 c          144 g          213 t
ORIGIN      
        1 aagtatatct gttcttcaag caaggaaaat cagccttaga atattatcaa cagtataaga
       61 aaaacaaatc aaactgtggt agacgtccgc ttgttttacc cgaggaacaa tcagaatata
      121 ttcaaagaaa ggttgttcaa ggatggacac ccgatgtgat tgttggtcgt gcagcgtttc
      181 ctattagttg ttctgctcgt accatttatc gtatgttcaa aaaggggcta tttgattctt
      241 ctgacttacc gatgaaaggc aagcgtaaac cgaatggaca tcaagaaaga cgtggaaaac
      301 aaactttccg ccgctctatt catgaacgtg aaaaggatta tagccaattc tcaaatgagt
      361 ttggtcacct tgaaggtgac actatcgtag gtctgaaaca taaaagtgct gtaattacct
      421 tagttgaacg attatcaaaa gttatcatca cattgaaacc gtgtggtaga caagcgattg
      481 atattgaaaa aaaattaaat caatggtttg aatctgtacc gaaaaaccta ttcaaatcca
      541 tcacttttga ttgtggaaag gaattttcaa attggaaaca gatcagtaat gtcaatgata
      601 ttgccattta tttcgctgat ccaggaacgc cgtctcaaag aggcctaaac gagaattcta
      661 acggattgtt acgtagagat ggtttattga aatctatgga tttcaattca gtagatgaat
      721 ttt
//
LOCUS       DANKKG010000339          721 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.348, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000339 DANKKG010000000
VERSION     DANKKG010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..721
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.348"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            66..683
                     /locus_tag="PW004_002978"
     CDS             66..683
                     /locus_tag="PW004_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727862.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
BASE COUNT          259 a           97 c          138 g          227 t
ORIGIN      
        1 ctaagatatt tggagttcag agagggcgcg tttgattttc aaacttcgca acagaaccga
       61 gaaccttggg ttttcaagtg ctttttggtt cattaatctt aaccatagtg tttttaaagt
      121 ggaaaacaag caacaaaaaa agtatgtgga gccttgtagg aatagttctt attattttta
      181 ctgtgactgc agcaacagaa gcaaattatt cgcataaaat tcagtcgtat aaaggtcgtt
      241 tagttatgac caaaaaagat gttacacgtt atacatcctc aagaactgga ggcctatatg
      301 actatgcacc attagatgca ttgaaatata aagatcattt aaagaaacat gaagtaaaag
      361 ttggtgatcg gtgggagaat gcatctcata aagcatataa ttccaaaata gtctatacag
      421 tagaagatgc cgatgagcaa aatatcacat tacctgtatt caattattgg ggaacagttg
      481 tgaaagtaaa tgggaaaaag agcactatag aaaatgacaa tggtttagtg tctattaaag
      541 gtacggggaa aagaactata gttgaggttt catctacgta tccaactacg atgattctat
      601 ctttattaat tagttcaata acttttatat tcttatttac acgttattta ataagatgct
      661 gcggtgaaat gaaaatagat tagatagata ttataaatat gttgattcta ttttcatagc
      721 a
//
LOCUS       DANKKG010000340          695 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.350, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000340 DANKKG010000000
VERSION     DANKKG010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.350"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            15..188
                     /locus_tag="PW004_002979"
     CDS             15..188
                     /locus_tag="PW004_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727863.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
BASE COUNT          234 a          116 c           95 g          250 t
ORIGIN      
        1 atgaggtatg tcctatgatg aaaagattgt actattcatt gatcataacg attggctatt
       61 tgattgtatc aaatctagga aatatggtat ttggtatcag taaagaattc agttggacta
      121 ctacattatg ggaatctcta ttctttttca tcttcgtatt tttgttacaa aattatcgaa
      181 aaaaataaac atgtgccatc tatctgttgg cacatgtttt aggatttatg gtacttttta
      241 ttcggcaatc cattttattt ttctacttct tcttaatacc tctaacactt ttgatttata
      301 aactaatcca atgcatctac ttgcgcttat taagctattt cagctagata gtaccacccc
      361 atgcgtcaat gcttgatttt ccttttgtat aacattgtat aagcaataaa ggacaaataa
      421 gtggtgatta caccaaacca ttgaataaga aaaattctga tacgtaaata aacatgaact
      481 tgaaaaaatt aatgaatcgg ttagctacta cgtagttgtc ttttttctca tacagttctg
      541 ttaacttaca cttctcttcg ctagataata aaaaatcctc caactgtatt gtccaatata
      601 atcccaggca tctaaactag cattatagaa gagacacaca tagaatctaa aataactggt
      661 tttaaatgtt tcttacaatc aaaaaaagct gttaa
//
LOCUS       DANKKG010000341          694 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.351, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000341 DANKKG010000000
VERSION     DANKKG010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.351"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(<1..314)
                     /locus_tag="PW004_002980"
     CDS             complement(<1..314)
                     /locus_tag="PW004_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727864.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     EF"
     gene            complement(321..>694)
                     /locus_tag="PW004_002981"
     CDS             complement(321..>694)
                     /locus_tag="PW004_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HDL2727865.1"
                     /translation="DEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKVD
                     WKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAAK
                     ELAKVNIVAGIAAAAASCALE"
BASE COUNT          221 a          148 c           80 g          245 t
ORIGIN      
        1 caaaattctc cccaaaaagg aactttttct agtgaaaact tggtaatctc tttgtaatta
       61 attcttgtta aagaataatt aaacacttca tttttacttg ttattttttc attgactata
      121 actagttcat ctggtaaaaa aattaaccaa caaacatcga caaacagtaa atgatataat
      181 ccatttccac ctccaagcat attcaatact ggtgaacatg ccttagaaac acgaatatag
      241 ttatggttta aatagatatt ttctttaaac attgcttgtt taaaagccat atgctcttgt
      301 tctaaacttt tcataaactt ttactctaat gcacagctag cagcagctgc tgcaattcct
      361 gctacaatat ttaccttagc taactcttta gctgcagctt ttcctaattg tttttctgca
      421 atttttaata ataaatccgc agctttttca aaattaaaag cttttaatgc ttcaataaaa
      481 tctccctgcg tacctatagc ccaagccact cctcctccag gaagagaatt aatggctaca
      541 catccagcat atcgtttcca atcaactttc tccattctag gaacaataat atgttcataa
      601 aagactcttg ctgctcttcc ttgttcgtta tcttgactga ttcgttcacg aatagcacca
      661 aaatccttta tatggtatac accatgttcg tcta
//
LOCUS       DANKKG010000342          692 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.352, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000342 DANKKG010000000
VERSION     DANKKG010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.352"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            94..>692
                     /locus_tag="PW004_002982"
     CDS             94..>692
                     /locus_tag="PW004_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDL2727866.1"
                     /translation="MHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQ
                     GYVSWGAGNANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTS
                     LFTKGIISHLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPV
                     IDPTRAGAANPTLTDGTNHAHIDQFGEIENANLHVAGWH"
BASE COUNT          233 a          125 c          145 g          189 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac at
//
LOCUS       DANKKG010000343          692 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.353, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000343 DANKKG010000000
VERSION     DANKKG010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.353"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..>692
                     /locus_tag="PW004_002983"
     CDS             <1..>692
                     /locus_tag="PW004_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2727867.1"
                     /translation="ATISRHSSGKYYISLLCKEEVRELPKSNSAVGIDLGIIDFAILS
                     TGQKFDNNKFTSKMEKKLKREQRKLSRRALLAKQKGINLFEARNYQTQKRKVARLHEK
                     VMNQRTDFLNKLSTEIIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSSFVSKLQYK
                     ADWYGRKIIKVDKWFPSSQICSNCGHKDGKKSLEIREWTCPICHTHHDRDINASINIL
                     TEGLRLHSMGLA"
BASE COUNT          247 a          117 c          124 g          204 t
ORIGIN      
        1 gctacaatat cacgtcattc tagcggtaaa tattatattt ctttgttatg caaagaagaa
       61 gttcgcgaac tacctaaatc taattctgct gttggtattg acctaggtat tatagacttt
      121 gcaattcttt ctaccggtca aaaatttgat aataataaat ttacgtccaa aatggaaaag
      181 aaactcaaac gtgaacaacg taagttgtca agacgtgcct tattagctaa acaaaaaggt
      241 atcaatctgt ttgaagctag aaattatcaa acacaaaaac gtaaagtagc acgattgcat
      301 gaaaaagtaa tgaatcaacg tactgatttt ctgaataagt tgagtacaga aattatcaaa
      361 aatcacgata tcatctgtat tgaagactta aacacaaaag gcatgttgcg taatcataaa
      421 ctagcaaaaa gtatttcgga tgtttcttgg tctagttttg tgtctaaatt acaatacaag
      481 gctgactggt acggtcggaa aatcatcaaa gtagataagt ggtttccatc tagccaaatc
      541 tgttcgaatt gtggacataa agatggcaag aaatctctcg aaattcgaga atggacttgc
      601 cctatttgtc atactcatca tgaccgagat atcaacgcta gtatcaatat cctgaccgaa
      661 ggtctaaggc tacactctat gggattggct ta
//
LOCUS       DANKKG010000344          685 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.354, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000344 DANKKG010000000
VERSION     DANKKG010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..685
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.354"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            339..>685
                     /locus_tag="PW004_002984"
     CDS             339..>685
                     /locus_tag="PW004_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDL2727868.1"
                     /translation="MKILIVDDEASILDIVEAYLSAKGYQVFRALSGNEALTKVDVVQ
                     PDLIVLDLMLPGLSGLEVCKKIRDSSTVPIIMLTAKTAEKDILEGLSLGADDYITKPF
                     SPKELVVRVETVLR"
BASE COUNT          218 a          120 c          167 g          180 t
ORIGIN      
        1 ttctacctta tttgatagta gatttaaaac tttgcaacag aaccagaaat gaatattgta
       61 aaaaatgatt aggtagcgtt tattaatcca tgttcataac ttaagacttt agacaacatc
      121 ccaaaagcag aagattctac tacgcctgac aaagtgatga cctttagtgg catgggaaac
      181 atggtcgcca ttgacggcaa acagttcgac atggaccgga tcgatttacg cgctaaggtc
      241 ggggaaaagg aagtttggga gattaaaaac acacgggaca tgatgggtca agttttagta
      301 gaatgaagat accaacgaaa ttggaggaga aagattacgt gaagatttta attgtggacg
      361 acgaagcaag cattctggat atcgtggaag cctatttgag tgcaaaaggc tatcaagtgt
      421 ttcgcgcttt gagcggaaac gaggctttaa caaaggttga tgttgtccaa cctgatttga
      481 ttgtgttgga tctcatgctg cctggtttat ctggcctcga agtctgtaaa aaaataaggg
      541 actcctccac tgtaccgatt atcatgttga ctgcgaaaac agccgaaaaa gatatcttgg
      601 aagggttaag cctgggggct gatgactaca tcacaaaacc attcagcccg aaagagctgg
      661 ttgttcgggt cgaaaccgtt ttacg
//
LOCUS       DANKKG010000345          671 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.355, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000345 DANKKG010000000
VERSION     DANKKG010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.355"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(<1..>671)
                     /locus_tag="PW004_002985"
     CDS             complement(<1..>671)
                     /locus_tag="PW004_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HDL2727869.1"
                     /translation="LISVLISKITKTIRVFYMMNFIVQIFYITLSSSRGAQVVICSFL
                     VLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFSFGTNYAKDFLATIPVSIDR
                     KVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIWKAALKVIRPTLLFGYGETD
                     FYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLVSSGIIAFICIYAFYVLNIF
                     SLLKT"
BASE COUNT          249 a          113 c           83 g          226 t
ORIGIN      
        1 tgttttgagt aaactaaaga tatttaacac atagaaagca tagatacata taaaagcaat
       61 tataccactt gatacaagta cttgaagaaa tccattatgc atgtttccat gtgctctttt
      121 tagttcattc acatctgtag tatcaagatc tacttttttc aaaaattttg catcgctatt
      181 cccatagaaa tcggtctctc cataaccaaa taacaaagtt ggtcttataa ctttaagcgc
      241 agctttccaa atcgtaaaac gaccagctga aatttcggag ttatcactag aatgctgtaa
      301 tattacttct ttttctggaa gtggagtttt atctgattca tttgactcta taacttctac
      361 ttttctatca atcgatacag gaattgttgc taaaaaatcc ttagcataat tagtaccaaa
      421 agaaaaatat accaatagaa ctaataatat gcttaccatc catttgattt ttacaggtag
      481 tacttctcta atgctctttc taaattttga attggcaaac aacaaaagaa ataatactaa
      541 aaaagaacaa attactacct gggcacctct agacgaagaa agtgtaatat aaaatatttg
      601 tacgataaaa ttcatcatat aaaaaacacg aatcgttttt gttatttttg aaattaatac
      661 actaattaaa c
//
LOCUS       DANKKG010000346          670 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.356, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000346 DANKKG010000000
VERSION     DANKKG010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.356"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            39..521
                     /locus_tag="PW004_002986"
     CDS             39..521
                     /locus_tag="PW004_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727870.1"
                     /translation="MNIKEERDSNLIQSLLNDLHRTFAQLPVELQPYAEMILNDVNNG
                     ELVIKEGWEFTDYLNEYQLSEEDDFIQDLVDSTNINEALLREMLDLRLTEVNINEYSR
                     FDKLKSSVNVGHFSGYIEKNLGKMVIPIKVNMLIDRLLRAFLLEDVFDVTEYIKNYFN
                     "
BASE COUNT          252 a           85 c          114 g          219 t
ORIGIN      
        1 ttaattatga gtatttaaac acaaacttca aaaaatacat gaatataaaa gaagaacgtg
       61 attcgaattt aattcaatca ttgttaaacg acttacatcg cacatttgcg caattaccag
      121 tggagttaca gccatatgct gagatgattc taaatgatgt aaataatggg gaattagtta
      181 tcaaagaagg atgggaattt actgattacc tcaatgaata tcagcttagt gaagaggacg
      241 attttatcca agatttagtg gattcaacca atattaatga ggcattatta cgtgagatgt
      301 tggatttacg cttaaccgag gtaaacatta atgaatatag tcgatttgat aaattaaaat
      361 caagtgttaa cgtaggtcat ttcagtggat atatcgagaa aaatttaggt aaaatggtaa
      421 taccaatcaa ggtaaatatg ctaattgaca gattattgcg tgcattttta ttagaagatg
      481 tattcgatgt tacagagtac ataaagaatt actttaacta gagtacacct acatcttata
      541 gtacataaaa aaattcatct tttttagttt aataattcac caaaaaggtg agtaatgcca
      601 tttttgaaaa ttgagcttgt tttatgtcta catctgtatc cgtatataat ccaaaggatt
      661 ataaactcag
//
LOCUS       DANKKG010000347          661 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.357, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000347 DANKKG010000000
VERSION     DANKKG010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..661
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.357"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..249
                     /locus_tag="PW004_002987"
     CDS             <1..249
                     /locus_tag="PW004_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDL2727871.1"
                     /translation="LDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESA
                     NDMYEKELKYFFENVVTGKENWNDLYHANEVLKIAEEK"
     gene            250..>661
                     /locus_tag="PW004_002988"
     CDS             250..>661
                     /locus_tag="PW004_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HDL2727872.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKD"
BASE COUNT          257 a           72 c          122 g          210 t
ORIGIN      
        1 ttagattatt ttggaagagt tcctacaaga aaatttcaat tgtttacttt agatgaatgt
       61 atcaatgttg acttattaaa taataaaata agttttgaag aaaacattgt taaagaaatg
      121 cctgaatctg ctaatgatat gtatgagaag gagttgaaat atttctttga aaacgtagta
      181 acaggcaaag aaaactggaa tgatttatat catgcaaacg aagtattgaa aatagctgag
      241 gagaaataaa tggaaatttt atttactatt tgtggtagag ctgggtctaa ggggtttaaa
      301 aataaaaatt tgaaatattt ggcaggaaag cccctagtcc atttcgcttt agcaatgata
      361 gatatctatc aacaaaaaca tcctgaacat aattgcacga cttgtttgaa tacggacagt
      421 aatgagttag tagaattgac gactaagtta aatccgtctg tgaaaattgt agaacgtaaa
      481 cctgaattag ctacagatac tgtagggaaa attgaagtga tacgtgattc attcttacaa
      541 ctagaaagag aagataatat atttgattta attatagatt tagatttgac ttcaccttta
      601 cgtgaagttt ctaatcttga agaattaata gatgaacatc taaaaaattt agataaagac
      661 g
//
LOCUS       DANKKG010000348          660 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.358, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000348 DANKKG010000000
VERSION     DANKKG010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.358"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            8..>660
                     /locus_tag="PW004_002989"
     CDS             8..>660
                     /locus_tag="PW004_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL2727873.1"
                     /translation="MKHKVSVIVPIYNVEKYLRKCVDSLLSQTLKEIEIVLVDDGSPD
                     ASGEIADEYQKKYSNVKTIHRENGGLGPARNTGIENATGEYVAFLDSDDWVESDMYEK
                     LYLVASKENADIVVSGHCDFANDKAMVKKQHPLSGNVYDTPNSIKNVRKNLFGHSPKD
                     QEVEAFPMSVCMSLYNKNLLDKYRLRFEKILSEDTIFNLNAYDVANKIAFTEYTDYC"
BASE COUNT          257 a           67 c          140 g          196 t
ORIGIN      
        1 aaaagttatg aaacacaagg taagtgtgat agtaccaatt tacaatgttg aaaaatattt
       61 aagaaaatgt gtggatagtt tattgagtca gacattaaaa gagatagaaa tcgtattagt
      121 tgatgatggt tcgccagatg cttcggggga aatagcagat gagtatcaaa aaaaatattc
      181 taatgtaaaa acaatacata gagaaaatgg ggggttagga ccggcaagaa atacaggaat
      241 tgagaatgcg acaggagaat atgtggcttt tcttgattca gatgattggg tagaaagtga
      301 tatgtatgaa aaattatact tagttgcgtc caaagaaaat gctgatatag tagttagtgg
      361 acattgtgat tttgcgaatg acaaagctat ggtgaaaaag caacatcctt tgagtgggaa
      421 tgtatatgat acgcctaata gtattaaaaa tgtacgaaaa aatttatttg gacatagtcc
      481 aaaagatcaa gaagtagagg ccttcccgat gtcagtatgt atgtctttgt ataataaaaa
      541 tctgctagat aaatatagac taagatttga aaaaatttta tctgaagata caatttttaa
      601 cttgaatgca tatgatgtcg caaataaaat tgcttttaca gaatatacag attattgtta
//
LOCUS       DANKKG010000349          659 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.359, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000349 DANKKG010000000
VERSION     DANKKG010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..659
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.359"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..329
                     /locus_tag="PW004_002990"
     CDS             <1..329
                     /locus_tag="PW004_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDL2727874.1"
                     /translation="FGEDIYKIGMTRRLEPMDRIKELSSASVPFEFDVHAMIFSEDAP
                     SLETKLHNHFRKQEVNKINQRKEFFKVSLDEIEKVVLENYNGTVTFTKLAKAEQYRRS
                     LELSKD"
     gene            401..>659
                     /locus_tag="PW004_002991"
     CDS             401..>659
                     /locus_tag="PW004_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727875.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYR"
BASE COUNT          281 a           83 c          110 g          185 t
ORIGIN      
        1 cttttggtga agacatatat aaaattggta tgactagaag attagaacct atggatagaa
       61 taaaagaatt gagtagtgca tcggttccat ttgaatttga cgttcatgcg atgatttttt
      121 cagaggacgc tccgtctttg gaaactaaat tgcataatca ttttagaaaa caagaagtta
      181 ataaaattaa tcaaagaaaa gaatttttca aagtttcact agacgaaata gagaaagttg
      241 ttttagaaaa ttataatgga acagtaacat ttacaaaatt agctaaagct gaacaatata
      301 gacgatcttt agaattgtca aaagattaaa tatataaaaa gccttcgggc tttttctttt
      361 aacaattaat aggaacgtat attcgaaagg aatagaaata atgtggttag aaaaaataaa
      421 tgacaaaaaa tttaagtaca ccgaacgtta tacggatccc ttaactgaaa aaaagagaaa
      481 agtgtccgtt actttatcta gcaattcacg ccaagcttgg aatcaagcta acttactttt
      541 gaatgaaaaa atcgcagaaa aaataaaacg aaatgaagaa ttaccactaa cttttggaga
      601 attaaaaact aaatgggatg aaaaatacaa gccaactgta aaagaaagtt cctatcgaa
//
LOCUS       DANKKG010000350          635 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.360, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000350 DANKKG010000000
VERSION     DANKKG010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.360"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            59..>635
                     /locus_tag="PW004_002992"
     CDS             59..>635
                     /locus_tag="PW004_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDL2727876.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFI"
BASE COUNT          160 a           93 c          140 g          242 t
ORIGIN      
        1 ttcaataatg tatgttatgc taagttcggg ctatatttta tacaaaggaa tgatgagaat
       61 ggaatttatt ggtattttat gcctgatatt gatttcaaca acgattggct cacatatctc
      121 gcggagatta ggtatcccag cagtgattgg acaattactt gttggtgttt tgctaggcca
      181 agctggattg aattgggtca aacctgatat tctagtccat gacttttcgg agattggtgt
      241 gatcttatta atgtttttag ctggtctgga aagtgattta tctcttttga aaaaatattt
      301 ccgacctggt atgttcgttg ctttgcttgg gatcatcttc ccggttgttt ttggctggtt
      361 gacaggcaaa gcattccaag tttcgggaaa tgaagcgatt ttctttggga ttattcttgc
      421 ggcaacttct gtcagtattt cagtagaagt attaaaagaa ctaaacgtag tgaatacaaa
      481 agaaggatca acaattttgg gtgcttctgt ggtagatgac attttagtcg ttttagttct
      541 aagtttcagc ctatcttttc taacggggga agcttctagc aatgcttctt taccgcttgt
      601 tttgattgaa cagctaattt attttgtttt tattt
//
LOCUS       DANKKG010000351          623 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.361, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000351 DANKKG010000000
VERSION     DANKKG010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..623
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.361"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(170..535)
                     /locus_tag="PW004_002993"
     CDS             complement(170..535)
                     /locus_tag="PW004_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727877.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
BASE COUNT          239 a           83 c          114 g          187 t
ORIGIN      
        1 tattattgga atcattattg ctgcaattat tacacaactg tttttagtgt ttggagagaa
       61 gaaaagaagt aaaaaataga aaaactagtg atagttttgg tgatataaga aaaacatata
      121 tgtataaaaa gaagaaagta gcttctcttt gtacatatat ggtttgtttt tatggatgcc
      181 gagctttttt ataagataag gctaccgccc ataacgaccc aaggatttgt ggaactaagt
      241 aaatacctgc aagaagcaag atcagctggg tcggttttaa aggatgatag aagtaacgcc
      301 attcagtaaa attggcgcaa aataaatagg aagtgacagt agatataata ttggcaatga
      361 ataaagatat aatacgcctg ttcctttgaa aataatatcc agcaaaaaga gataaaaata
      421 tcaatgtgat gaacccgctt acggaatgat aacgaaaatc aagcagtaag gcgaaatgca
      481 cataaggaac aaaagataaa agcaacggaa ggtttttcca gcaagttttt atcatctttc
      541 atcaccacct aacaagtgtt attattttgt tagttttatt gtaaattgaa tcctgcaata
      601 atcaattact ttttcattat ttc
//
LOCUS       DANKKG010000352          614 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.362, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000352 DANKKG010000000
VERSION     DANKKG010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.362"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(<1..>614)
                     /locus_tag="PW004_002994"
     CDS             complement(<1..>614)
                     /locus_tag="PW004_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="capsular polysaccharide synthesis protein"
                     /protein_id="HDL2727878.1"
                     /translation="HLLKKHEVMLKYYEKTFSDFLKTYDYSQNQIINQSKSPYSNCIW
                     VCWWQGLDQSPEIVKACVKSIKRNAGKHTVIILTEDNYRQYVDIPKWVEEKKNKGIIT
                     RTNYSDLLRLSLLAKHGGLWLDATFYCTKPIPDEYFDIDIWSIRRPDYAHASVASGYF
                     AGYSLACNQESRWMFAIIRDFFLNYWKNNDMMVDYLMIDYMIVL"
BASE COUNT          205 a          115 c           68 g          226 t
ORIGIN      
        1 taaaacaatc atataatcta tcatcaaata atccaccatc atatcattat ttttccaata
       61 atttaaaaaa aagtctctaa ttatagcaaa catccatcta gactcttgat tacatgctaa
      121 tgaataaccc gcaaaatatc ctgatgctac ggatgcgtga gcataatcag gcctcctaat
      181 agaccaaata tcgatatcaa aatactcatc tggtattggt tttgtacaat aaaatgtggc
      241 gtctaaccac aaacctccat gttttgctag caaagataat cttaataaat cagaataatt
      301 tgttctcgta atgattcctt tgtttttctt ttcttccacc cattttggta tatcaacata
      361 ttgtctataa ttatcttctg ttaaaattat aacagtatgt ttccctgcat ttcgttttat
      421 tgattttaca caagctttta caatttccgg cgattgatcc aatccctgcc accaacaaac
      481 ccaaatgcag ttagaatatg gtgattttga ttgattaatt atttgatttt gactataatc
      541 atatgttttc aaaaaatctg aaaaagtctt ttcataatac ttcagcatta cctcatgttt
      601 ttttaataaa tgtt
//
LOCUS       DANKKG010000353          613 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.363, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000353 DANKKG010000000
VERSION     DANKKG010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.363"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(<1..>613)
                     /locus_tag="PW004_002995"
     CDS             complement(<1..>613)
                     /locus_tag="PW004_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HDL2727879.1"
                     /translation="LYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTG
                     NLSKTIEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPS
                     VSGDTIRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVR
                     DSGIVYYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIA"
BASE COUNT          206 a          102 c           76 g          229 t
ORIGIN      
        1 agctatttcc tttacagtaa ttcttttata gatatttaag ccgatgaatt gaacgacaga
       61 agaaattaag ggagaaagat gctctatctt agtttttccg atttccttag tataataaac
      121 aatcccagaa tccctcactt ttaagacctc aggtaacgat acagctaatt cgttttcttg
      181 tatcaaagca tctctacttt gatacgcttc tattatatcc attcctaatg ttatagacgt
      241 ttgtgctaac ttttcaaaaa caatgataga ataatttttt tcagaccgaa tcgtatctcc
      301 actaacactt ggaattattc cattactcag attaaaaacc atgttttcta aatttcgaat
      361 atctccattt ttaacatatt ccagtatttt attttcataa agaaacagat atatttctgg
      421 atcgtaattt tgtgataaca gattatctat tttgatttgc tcgatagttt tacttagatt
      481 tcctgtatat tcaattaacg gttcatggaa tagatcttct atttttccag taaaaaaata
      541 atgaactaaa atcagaatat ctcgaatgtc tcctaaagaa tacaaagtca aattttgcaa
      601 tgaattatag agt
//
LOCUS       DANKKG010000354          600 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.364, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000354 DANKKG010000000
VERSION     DANKKG010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.364"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            117..452
                     /locus_tag="PW004_002996"
                     /pseudo
     CDS             117..452
                     /locus_tag="PW004_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313007.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          203 a           63 c          105 g          229 t
ORIGIN      
        1 tttatggttt attgaaataa ggttgcgaag gaagatgtag agttatttgc actgttgtgt
       61 tggtatatct cttaatgctg tgtctttttg gggagattaa ttagtggagt gattatatga
      121 gtatagatga ttgtttagaa gaacaaataa gattattttt aaagttttta aaagttaaag
      181 ttcaaaataa ctacttcaca tgttatagtt atgcacaccc tgttttaaat agtcttcctt
      241 ttaattcttt agtgcaagga ttgattacaa atattgtttt attagtctgt acaccaaatg
      301 aaatatttgt aaaaaagata ggcagtggta tttcttcagt aagattaaaa gagaaagaat
      361 atactcaaaa gattattcat attcctaagc ctgaaattaa gcgttttgag ataaaagact
      421 attctgcttt agggctgttc tcaattggtt actggtgata aaaaataaga atactaaaac
      481 tatacgtaga atataagtaa tcggcatcaa tcctaattat gtgattgaat gccgattttt
      541 gttacgcgct atttttaatt atctgtaaga agaataattt tattttactc atattcgttt
//
LOCUS       DANKKG010000355          597 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.365, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000355 DANKKG010000000
VERSION     DANKKG010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.365"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
BASE COUNT          224 a           55 c          104 g          214 t
ORIGIN      
        1 aaatgagcgt aaaaacggct atttttgcct taacaacgat ttttttgtat acaaatacga
       61 tatgctcatt aattattgga atttgtagtg ctgtttaaat taatgaaggt gtaattatat
      121 gagtgatcat tttgaaaaag tttgttgaga agatgtgctt ctagtcatgt gatgggagaa
      181 aataatttaa ctgttagagt gaatagttaa attattctgg ttatatgaaa aaagtagttt
      241 ctaaaataat cttgcaggga ttaacttaaa gaaattgaga gaatagtttc tttgaaattt
      301 ctatatacaa tgagaagctg aaatttactt ttatttgatg actggaaata gaaattaaat
      361 atattgtatt gttacattaa agaaaacttg tatggaaaaa tcagtacaat accattattt
      421 aatgttcttc acttaaagcg caaaaaatat cagtttatta aactcgtata tagaacaaat
      481 atatgtagac ggggcgaaac gatgagtgta aaaaaaatgc tatttatggt aagtacaata
      541 attgtattaa catcccgatt ttggggaatt aatactagtt tatctatgcg atatttt
//
LOCUS       DANKKG010000356          596 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.366, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000356 DANKKG010000000
VERSION     DANKKG010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..596
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.366"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(80..>596)
                     /locus_tag="PW004_002997"
     CDS             complement(80..>596)
                     /locus_tag="PW004_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2727880.1"
                     /translation="IQDFQFWLRRTSNPERDSFLFLFGINNGLRMSDIIGLKVGDIRG
                     KSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQGRHITRDRVYQIY
                     ADVAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQAITKRYIGITEDEI
                     GASLRGFKLGG"
BASE COUNT          181 a          126 c           60 g          229 t
ORIGIN      
        1 ttccgaaaat tcagttatct tcattaatct caccttctaa gttttcatta tacacaaaaa
       61 atatatccta atatctcaat tagcctccca atttaaaacc acgtaacgat gcccctattt
      121 catcttcagt tataccaatg tacctttttg taattgcttg cgagctatga ttaaaaataa
      181 acattaaggt agcaatgtct ctagttttct tgtagtagtg atacccaaaa gtttttctta
      241 atgtatgtgt tcctatatca tctcttccta atttttctgc tacatctgca tatatctgat
      301 agacacgatc ccttgttata tgtcgacctt gttgccgact tggaaataac caatcatttt
      361 cttctttacc ttcagtatat aataaaatct cttctctcaa attatctata aaaatcggtt
      421 ttcgcttacc tgttttatgt tcaacaataa taggcttact tttacctcgt atgtctccaa
      481 cttttaatcc tataatatct gacatacgta acccattatt gattccaaac aaaaataaaa
      541 aactatctct ttctggatta cttgttcttc ttagccaaaa ctgaaaatct tgtatc
//
LOCUS       DANKKG010000357          581 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.367, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000357 DANKKG010000000
VERSION     DANKKG010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.367"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..>581
                     /locus_tag="PW004_002998"
     CDS             <1..>581
                     /locus_tag="PW004_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727881.1"
                     /translation="VHDLTFAMGVFFLYYVFFENKNTKKHKIKIIVSLILIFMGLKRI
                     ELLALSGAILVYYVLLKWGKKIQIRALVCTICATVISLVFIYIIDTGILALLMSNLGI
                     TDDGARLSFYLYSSKFYELGLGYVGTGWTWFSKYFFNLYLSHFRLNGGRIAASLHSDI
                     LTIYIEVGCIVFIIWMFWLFFVKPIRLTKKFGL"
BASE COUNT          183 a           57 c          109 g          232 t
ORIGIN      
        1 ggtacatgat ttaacttttg cgatgggggt ttttttcctt tattacgtat tttttgaaaa
       61 taaaaataca aaaaaacata agataaaaat tatcgtatct ttaattttaa ttttcatggg
      121 attgaagaga attgaactgt tagctttaag tggtgcaatt ctagtgtatt atgttttatt
      181 aaagtgggga aaaaagattc aaatccgtgc attagtgtgt actatttgtg caactgttat
      241 ttcgttagtg tttatttaca ttatagatac aggaattttg gcattgctta tgtcaaattt
      301 gggcattaca gatgacggtg caaggctttc attttatcta tattcatcta aattttatga
      361 acttggttta ggatatgtag gaacaggatg gacatggttt tcaaaatact tttttaatct
      421 ttatttatcg cactttaggt taaatggtgg aagaattgca gcaagcctac acagtgatat
      481 attaactatt tatatagaag ttggatgtat agtttttatt atatggatgt tttggttatt
      541 ttttgtgaaa ccgataagac taactaaaaa atttggattg a
//
LOCUS       DANKKG010000358          575 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.368, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000358 DANKKG010000000
VERSION     DANKKG010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.368"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(189..>575)
                     /locus_tag="PW004_002999"
     CDS             complement(189..>575)
                     /locus_tag="PW004_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDL2727882.1"
                     /translation="NIIEELSKNSRITMKELGEKVHLTGPAVSARVTKLEESGVIENY
                     TVKLNMEKLGFSIHAFLIIITQNLNHKPYLSFIKNHEKFLIRNYKISGGGCYLLECRF
                     TSKKSMNSFLEQLNEYANYKLSIVID"
BASE COUNT          183 a           87 c           71 g          234 t
ORIGIN      
        1 tttggccttc atagaatttt ctaaatatga atcatcaatg aatttaatta aaaaaataag
       61 tagggcaaaa tataaatgtt tttgccttac ttgtttccat cagtttagtt ttaaattgat
      121 aagttatata gttatggttg aatgtgtgtc tcgcatatta gtatttttac catctgctag
      181 ttttattatt aatctataac aatagataat ttataattcg catattcatt aagctgttcc
      241 aaaaagctgt tcattgattt ttttgaagta aatctacatt ccaaaagata acagccccct
      301 ccactaatct tataatttct aatcaagaat ttttcatgat ttttaataaa tgataaatag
      361 ggtttatgat ttaaattttg cgtaattata attaaaaaag cgtgaatcga aaaccctagc
      421 ttttccatat ttaattttac tgtataattt tcaataactc cactttcctc tagttttgtc
      481 actctcgccg agacagcggg tccagtcaaa tgtacttttt ctccaagttc tttcattgtg
      541 attcgactat tttttgatag ttcctcaatg atatt
//
LOCUS       DANKKG010000359          573 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.369, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000359 DANKKG010000000
VERSION     DANKKG010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.369"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
BASE COUNT          185 a           81 c           81 g          226 t
ORIGIN      
        1 tttttcttga tgtgatatcc ttgtcttttt tgtaatgttt ttaacttatt gcttttgcaa
       61 aatttattta cctgttttct ggatcaacga tagtgtacat tatgctatcc ttgtttggat
      121 tttaggacgt ttaaagaggc acttttttac ataacttaat ccatagacct tttaagcatg
      181 ctgtgagata ataataaaga agagtttaaa gcgcacccca aaccacttcc ccataagtgt
      241 attacgcttt aaactctttt atatttgaat tcactaagaa gcataccata tttttgaaaa
      301 aaagtgagaa aaaaggctta taattaggat ttagagtaat taattagatg tttattttat
      361 aggactgata ctatagatta caaatagtat cgtatttcgc aatcttaaaa ttatcttctt
      421 actgtgaata agataataaa atttgtattt gtccttttta ggtaacttat tttaatctat
      481 atacctttct ggatcaacga aagtatactt tatatagtca taacgccgat ggtaactatg
      541 cactgtaacg ataatgctgc taaaatatct ttt
//
LOCUS       DANKKG010000360          570 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.370, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000360 DANKKG010000000
VERSION     DANKKG010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.370"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(1..570)
                     /locus_tag="PW004_003000"
                     /pseudo
     CDS             complement(1..570)
                     /locus_tag="PW004_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319352.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
BASE COUNT          166 a          135 c           74 g          195 t
ORIGIN      
        1 ataattaata gcttcagtta agattgccaa actggtacct ccactactac caaatacacg
       61 tataggtacc atcgtaacat cccaatttat tccagcaaca cctaattcgt tgtttgtttg
      121 agcaccaata atcccggcaa tatgtgtcca tgtcccatag tatccatagg atctgtatca
      181 tcgttaaaaa aatcgtatct caaactttca tctacatttc cagaaagatc ttcatgttga
      241 taatcaattc ctgaatcaat gaccgctacc ttcacatcct tagaaccctt ggtttcttcc
      301 caagcggatg gcatgctaat ctctcttaaa ttagcttgtt tactccaatt aggatcgtta
      361 agaataattg atcgatttcc taaagaatca gttgtatttg gctcctcaac atcattcaac
      421 ggctctagtt cagccacata gtttggctct gcataattga ccatatcaga agcattaaat
      481 gtttttactg cctcttccac actaatttct tcgttcaatt tcactaataa tacttgatct
      541 cgcttattat aatcgctttc atttgtccta
//
LOCUS       DANKKG010000361          568 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.371, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000361 DANKKG010000000
VERSION     DANKKG010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.371"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
BASE COUNT          196 a          104 c           60 g          208 t
ORIGIN      
        1 aataatttct aaatattctt taaaaaagct tataactatt atgccttttt taatatataa
       61 cacagttatt tttaaaaata agaatgctaa atcactctac aataacggcg aaagcacaaa
      121 aaataaaaaa attattacgt tattaaaaaa acagatgaaa gatcaggttt tattagatct
      181 cattctttca tcacttgaag ggatttaatg aactactttt tcctctttgt cgtttttata
      241 tattcgtatt ccttcaaaca gtaggatagc ctctttcaat gcttgatatt tgaactgtcc
      301 gttacttaaa aatttagatg tgccgccctc ttcctagaac atattctttt ttactttttc
      361 attaattgac agaccaatcg aacgaaactg taaatttcga taggataatg attggtcaaa
      421 ctcattcatt ttcaagacat ctcgattcat attcaagata ggaatctttt tatcaacatc
      481 actttattcg tattttcctc taccataaaa gcttcacgct ccacaaaatt gttttataca
      541 ctactattca gcccattcaa cgaccaat
//
LOCUS       DANKKG010000362          562 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.372, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000362 DANKKG010000000
VERSION     DANKKG010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..562
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.372"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            66..>562
                     /locus_tag="PW004_003001"
     CDS             66..>562
                     /locus_tag="PW004_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HDL2727883.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDAT"
BASE COUNT          208 a          103 c           93 g          158 t
ORIGIN      
        1 aaaaggaagt cccttctgta gaataaagtt aacgacaacc aattcacaga aaagaggact
       61 tccctatgaa tgattttact acagaaattg tgcaaactct agtcactaaa ggcgatttaa
      121 atgaattatt ccgttcgcac ttagaaaaag cgataaacac actcctacgg actgaattaa
      181 cggctttttt agattacgaa aaatatgatc gcactggttt taattcaggt aattcgagaa
      241 acggttctta ctttcgatca atcaaaaccg aatatggtga attaacattg gaaataccta
      301 gagatcgtaa tggtgagttt aaacaacaaa ctttaccagc ctacaaaaga acaaacgata
      361 cattggaaac cactattatc catttattcg aaaaaggtgt tacgatgtct gaaattgctg
      421 atttgatcga aaaaatgtac ggtcatcact atactccaca aaccatgtcc aacatgacta
      481 aagttctgac tgaagaagta aatgccttta aatccagagc cttaaatgat aagtatgtcg
      541 ctatttttat ggacgctact ta
//
LOCUS       DANKKG010000363          550 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.373, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000363 DANKKG010000000
VERSION     DANKKG010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.373"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..298
                     /locus_tag="PW004_003002"
     CDS             <1..298
                     /locus_tag="PW004_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HDL2727884.1"
                     /translation="AKITETYPQLDTTVKWNQPMFTDHGTFIIAFSFAKNHFSIAPEK
                     AAIRALEKNIQEAGYVYTDNVIKVPWKSVINWELIEQLISFNIEDKKGHDKFWR"
BASE COUNT          192 a           92 c           86 g          180 t
ORIGIN      
        1 tgctaagata acagaaacgt atccccagct agacacaact gtaaaatgga accaacctat
       61 gtttactgac catggtacct ttattattgc atttagtttt gctaaaaatc atttttctat
      121 cgcaccggaa aaagcagcga ttcgtgccct tgaaaaaaat atccaagaag caggctacgt
      181 atatacggat aacgtcatca aagttccttg gaaaagtgta atcaactggg aattgattga
      241 acaattgatt tcatttaata ttgaagataa aaaaggtcat gataaatttt ggcgataaag
      301 ctttatacga ataaaccttt tactggcgag gaaactattc atcaccttat acaggttttg
      361 ttcaatccta tcctttttat catccctggt taaacactta attgacaaag tatctgctat
      421 ttttataagg agtctttctt gtgctgaaaa aaatcactga ttgaatggct ttgaattttc
      481 tacttagtaa aattattttt tcagcaattt tcctgcaaaa cttttgaata aggaaaaaat
      541 acgagaaaat
//
LOCUS       DANKKG010000364          546 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.374, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000364 DANKKG010000000
VERSION     DANKKG010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.374"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(65..514)
                     /locus_tag="PW004_003003"
                     /pseudo
     CDS             complement(65..514)
                     /locus_tag="PW004_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          159 a          112 c           68 g          207 t
ORIGIN      
        1 agccataaca ccgcagcatt cacaacacct tataattttt gtcaaaaaaa gtgttgccat
       61 tccacttttt taaatatgcg ttctctgctc ttaaacgaat aacttcttct tcaagagact
      121 catctttaag ttttttagga atgtttagct tggaattcat actaattttt ctgccccttt
      181 tttggctctc aagtgaagaa gcaccgcctt cttcatattg ctctatccat ttacttagag
      241 ttctatttga accgatatta aattttttag cagtttcttg gatagaaaga ccatttgttt
      301 ccatatattc tataacatca agtttaaatt ttgtagtgta gcttttgcca cctccaacca
      361 agccttccca accatgatag ttataaatcc ttacccaatg tctaaccagt gtacgattta
      421 tttgatattt atgtgcaaga tatttgtagc cgccttcgtt atttaaatag tctgaaacta
      481 cttttttctt aaaaacaaat gtatatttcc gcaaaaaaag cacccctttt aattagattt
      541 ctagtc
//
LOCUS       DANKKG010000365          546 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.375, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000365 DANKKG010000000
VERSION     DANKKG010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.375"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..>546
                     /locus_tag="PW004_003004"
     CDS             <1..>546
                     /locus_tag="PW004_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK signal domain/LPXTG anchor domain surface
                     protein"
                     /protein_id="HDL2727885.1"
                     /translation="IFTAGEGTSIESGTTVFAVKDGVSLPEDKLPVLKAKDGYTDAKW
                     PEEATQPITADDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEPDASEGIKNKEDL
                     PKDTKYTWKEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG
                     EKVEVGGTVDLTDNVTNLPTLP"
BASE COUNT          219 a           86 c          126 g          115 t
ORIGIN      
        1 atcttcaccg caggggaagg aacaagtatt gaaagtggaa caacggtctt tgcagtgaaa
       61 gacggcgtaa gcttaccaga agataaactt ccagtgttga aagcaaaaga tggttataca
      121 gatgcgaaat ggccagaaga agcaactcaa ccaattacag cagatgatac agaatttgta
      181 tcaagtgcaa caaaactgga tgataaatct gacgctgaca aatataatcc tgaaggtcaa
      241 aaagtgacta cagaattgaa taaggaacca gacgcatctg agggaattaa gaacaaagaa
      301 gatttaccaa aagatactaa gtatacttgg aaagaaaaag tagatgttag cgcagctgga
      361 aataaaaaag ggactgttgt agtgacatat ccagatggat catctgatga agttgaagta
      421 gatgttacag tgacagacaa tcgttctgac gccgataaat atgagccaac agtagaaggc
      481 gaaaaagtag aagttggagg cacagtagat ctaacagata atgttactaa cttgccaaca
      541 ttacca
//
LOCUS       DANKKG010000366          540 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.376, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000366 DANKKG010000000
VERSION     DANKKG010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.376"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            288..>540
                     /locus_tag="PW004_003005"
     CDS             288..>540
                     /locus_tag="PW004_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDL2727886.1"
                     /translation="MAKITKNEQENLVENYTKKPAYSNSRLDAHFVCTSNPKLSFDAM
                     TIVGNLNKNSAKKLSDFMSLDPQIRLWDILQTKFKAKALQ"
BASE COUNT          210 a           79 c           87 g          164 t
ORIGIN      
        1 ataaattatc taaagagaat taagttttga ttatgtaata agttttggta ctaaaaaaga
       61 cttgatctaa ttagaccaag tcttttgtta gtgttatatt aataacaaaa taaaaataag
      121 aagtcgctca ctccccgacc aaagtttgtg agcaaaataa acagtcgatg tttgtttctt
      181 aattgtatat cttgatatta aacgatttaa gattattctt caagatatat attcgcaaat
      241 gtaattggaa aagcgacttc ttaaacaaaa ttaaggagtc gctttttatg gcaaaaataa
      301 cgaaaaatga gcaagaaaat ttggtggaaa attacacaaa aaaaccggcg tactctaata
      361 gccggttaga cgcacatttc gtgtgcactt ctaatccgaa attgagcttt gacgctatga
      421 cgatagtcgg aaaccttaat aaaaacagcg ctaaaaagct atccgatttt atgagtttag
      481 atccacaaat tagactttgg gatatacttc aaacaaaatt taaagctaaa gcactacaag
//
LOCUS       DANKKG010000367          530 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.377, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000367 DANKKG010000000
VERSION     DANKKG010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.377"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
BASE COUNT          167 a           75 c          136 g          152 t
ORIGIN      
        1 gaccaaaacg gtgtactgat tggtcgaaga aaaaatggag tcttgcgttg taccgcaaaa
       61 gggatggggc gtttaatcag ctgaattgat cattctcata acttgtattt tatcgttttt
      121 cgcttgcttg aaaatattgt cgagacttct aaaagaactt agaattacta gaaaaataag
      181 gagtagactt gatctatcaa caattccttt cggggggtga cggggtaggt ttttacgatt
      241 ggtaacgtaa aaaattggtc aatcgagtaa atgcccttgt ttggatagtg gtgaaatgaa
      301 tttgtgtaaa taatatgcac agagtaaaca agagggaatt tagaagttag acaagttaga
      361 atagcgccta gattaatcat gagcagctac taagggatct aggctttgac gcaaactcat
      421 ggaataagga gtaggtcttc tcgtaatggg gaggacgcac aagaaattgt gtcgtgtgct
      481 gcagtacatg tagatacttg cgaggacgtt ggtcattgga tcaatcgagc
//
LOCUS       DANKKG010000368          529 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.378, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000368 DANKKG010000000
VERSION     DANKKG010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.378"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..468
                     /gene="scm"
                     /locus_tag="PW004_003006"
     CDS             <1..468
                     /gene="scm"
                     /locus_tag="PW004_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HDL2727887.1"
                     /translation="TSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTS
                     DSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNF
                     NLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT          196 a          117 c           89 g          127 t
ORIGIN      
        1 acatcagctt caagtgacag tacagatact acctctacca caagtgatag cacggacacg
       61 tcagcttcaa gtgacagtac agatactacc tctaccacaa gtgatagctc agattcatct
      121 accactacaa gtgatagcac ggacacgtca gcttcaagtg acagtacaga tacatcctct
      181 accacaagtg atagctcaga ttcatcaact tcaagcgata gtacagacac gtcgtctacc
      241 acaagcgata cacgtcatgc aagtgtctcc ttctcaaaaa caagttcgga taacaggacg
      301 aactttaact taactaataa aacaagcaat gacaatggaa acaaatcaaa aagagcactt
      361 cctaaaactg gaagtcaatc gaataattgg ataacactcg caggcgttat attacttgta
      421 atcggtttaa gaatcacttt tatgagttat aagaaatcga aaaggtaaaa aaatattcaa
      481 gataaaaaag agcaacgtcg ctaacggacg ttgctcttta tttttgctt
//
LOCUS       DANKKG010000369          520 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.379, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000369 DANKKG010000000
VERSION     DANKKG010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.379"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..>520
                     /locus_tag="PW004_003007"
     CDS             <1..>520
                     /locus_tag="PW004_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDL2727888.1"
                     /translation="SINRLKHWVSLTKELSNPSLKREIEYSQSEDLLKALKWSEEVNQ
                     QISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEEWERHELLSLVDELCCQ
                     DKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFFYPILVNKEKESWENLR
                     TDVLFKCLSGDYV"
BASE COUNT          203 a           52 c          105 g          160 t
ORIGIN      
        1 tcgattaatc gattaaagca ctgggtatct ctaacaaaag aattgtctaa tccatctttg
       61 aagagggaaa tagaatattc tcagtcagag gatttattaa aagctttaaa atggtcggaa
      121 gaagtcaatc aacagattag tcaagcaaaa ggattagata aacccttttt aagtgctaga
      181 gatactataa aagctctgaa aaaatatggg aagataatta ttgtaagctc agctaataag
      241 gaagctgttc aagaagaatg ggaaagacat gaattattat cattagttga tgaactgtgt
      301 tgtcaagata aaggaaaaaa agaagatatt attcgaagtg taattgaaaa tggttgtgat
      361 ttggataaga tattaatgat tggtgattct cctggtgatc tagaagctgc aaataaaaat
      421 aaagttttct tttatcctat tttggtcaat aaagaaaaag aatcatggga aaacttaaga
      481 actgatgttt tatttaaatg tttatcggga gattatgtag
//
LOCUS       DANKKG010000370          509 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.380, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000370 DANKKG010000000
VERSION     DANKKG010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.380"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..385
                     /locus_tag="PW004_003008"
     CDS             <1..385
                     /locus_tag="PW004_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2727889.1"
                     /translation="FLILSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYA
                     VYSFARKIGQALAGGVGGWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGL
                     ILFFLYPLSKDKVLENCELLKQKHK"
     gene            395..>509
                     /locus_tag="PW004_003009"
     CDS             395..>509
                     /locus_tag="PW004_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDL2727890.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHH"
BASE COUNT          158 a           63 c          102 g          186 t
ORIGIN      
        1 attcttaatt cttagttcgg ttggctatat gggtttgggt gtgtttaata ctgttatttg
       61 ggcaaatatt actgatgtta ttgatgatca agaagtaaag tctgatcaaa gagaagatgg
      121 gaccgtatat gcggtatatt cttttgctag aaaaataggt caagctctag ctggaggcgt
      181 cggaggttgg actctttcta ttataggcta tgactcttta gcaaatgtac aaacgggaga
      241 tgttttagaa aagttatata acgcttcaac attaattcca tcaatttgtt ttctgattgt
      301 aggattaatt ttatttttcc tttatccatt atctaaagat aaagtcttag aaaattgcga
      361 gcttttaaaa caaaaacata aataggagag aaatatgaat aattggatat tttgtattga
      421 ttcagatggc tgtgttatgg atacgatgac ttataaacac aatctctttt tcggaccaat
      481 tgctgcagag atttttgatg ttcatcata
//
LOCUS       DANKKG010000371          494 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.381, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000371 DANKKG010000000
VERSION     DANKKG010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.381"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(<1..>494)
                     /locus_tag="PW004_003010"
     CDS             complement(<1..>494)
                     /locus_tag="PW004_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2727891.1"
                     /translation="DQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQM
                     SIHLLSLLVTYCLQKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPII
                     RYLEEHLSENFLLEQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLEN
                     ELTY"
BASE COUNT          164 a           62 c           80 g          188 t
ORIGIN      
        1 ataggttaat tcattttcta gcacgagacg ttttgctttt tccattttta gttctttaaa
       61 atagctaata acactttttc cattcacttt tctaaaatgt ttttttaagc tggctggact
      121 gataaaaaaa atttgggcta attgttcaag caaaaaattt tctgaaagat gttcttctaa
      181 atatcgtatg attggatcaa ctaattcatc tgaataaaga atatgctttt ttttaggaat
      241 gactttctca tttgaaggta ttgtcaataa agcaagttcc tgttgataat gccttggtaa
      301 ggaagacaat tgaatttttt gcagacaata agtaactaat aaggaaagca aatgaatgct
      361 catctgttgg catagtatga aaaaagaatt gatttcctga tcataggaaa tagctggctt
      421 actatgtttt agataatcaa ttttgaactg ttcatttttc agaaaagtaa tgatagatgt
      481 tatttcttga tcta
//
LOCUS       DANKKG010000372          493 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.382, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000372 DANKKG010000000
VERSION     DANKKG010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.382"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..365
                     /locus_tag="PW004_003011"
     CDS             <1..365
                     /locus_tag="PW004_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2727892.1"
                     /translation="VFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCYTLLRPDGKTM
                     FYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRACHVYEKLGGK
                     NDYKDEIVYVYDYEKGDK"
BASE COUNT          196 a           35 c           84 g          178 t
ORIGIN      
        1 tggtttttga tattgataat ttaaaaggat ttcttaatga taccagttca tttgggttta
       61 tagctaaaga aaataataaa attataggat ttgcatattg ctatacactt ttaagacctg
      121 atggaaaaac aatgttttat ttacactcaa taggaatgtt acctaactat caagacaaag
      181 gttatggttc aaaattatta tcttttatta aggaatattc taaagagatt ggttgttctg
      241 aaatgttttt aataactgat aaaggtaatc ctagagcttg ccatgtatat gaaaaattag
      301 gtggtaaaaa tgattataaa gatgaaatag tatatgtata tgattatgaa aaaggtgata
      361 aataaatgaa tatagttgaa aatgaaatat gtataagaac tttaatagat gatgattttc
      421 ctttgatgtt aaaatggtta actgatgaaa gagtattaga attttatgat ggtagagata
      481 aaaaatatac att
//
LOCUS       DANKKG010000373          493 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.383, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000373 DANKKG010000000
VERSION     DANKKG010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.383"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            411..>493
                     /locus_tag="PW004_003012"
     CDS             411..>493
                     /locus_tag="PW004_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2727893.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRV"
BASE COUNT          159 a           84 c           86 g          164 t
ORIGIN      
        1 tttatcacgg atgagcttga aatcgtcttt tatacgtatc aaattgaaat gttcggcata
       61 caacctatcg ttgttactac attactaatt gtcacttgtt aaggaacttg gatcctttct
      121 aaacattccc tagagataaa ttttacttta tccctccgtt aatatccaat ttaagcacct
      181 ttccgattca gggttaattt gcatatcaag tgcagcttgt gttctagtaa gattagccct
      241 tttagtccgg ctgagttgac ttatggatat aagatatggt catagcaaat agatactacg
      301 tatagtctgt taatttagtg tcattaatct taagttggat aggctacaat taactagaca
      361 gaaaaattaa ggtgtgtaga ctagaagaaa acataccagg aggaattttt atgtctaaga
      421 gaacacgaag aactttttca caagaattca agcaacaaat cgtcaatctt tacttagctg
      481 gaaagccacg tgt
//
LOCUS       DANKKG010000374          482 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.384, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000374 DANKKG010000000
VERSION     DANKKG010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.384"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
BASE COUNT          180 a           65 c           76 g          161 t
ORIGIN      
        1 aattcaatta taaagaaaaa gaatcaataa tttaatgagg ctgtgacaaa tgtcattttt
       61 tgtttcgcta aaaacgagtg aaaaaatgtt gataactcca atcattcgtt taagattcta
      121 ggaatcattc aggaatgctg tgaaaccgat actttataat tataaagaag gtgatttcaa
      181 attccgaaga aggaatttga agtcaccttt ttacttatgt cacagtctca ttcagtaatg
      241 cgggataagg ctccttattc cgctatttga atactctgaa gtttaatcta agaaagagta
      301 atattttgaa aacatgcttc atcgcataga aatgaactta atttcagaaa gaatacatac
      361 atatcttttt atatgttttc agctgctgaa tggttaaaaa gcacaaatat ccgaaaaaaa
      421 catgaagaaa tgtacttttt ttgttgtaat gtgcatgaaa ttctttaagt aaactagtta
      481 ac
//
LOCUS       DANKKG010000375          463 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.386, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000375 DANKKG010000000
VERSION     DANKKG010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.386"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            165..>463
                     /locus_tag="PW004_003013"
     CDS             165..>463
                     /locus_tag="PW004_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727894.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSMGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYY"
BASE COUNT          149 a           63 c           54 g          197 t
ORIGIN      
        1 cttttttact tattttcata gatttgttgt tctccttttt taattctatt ttcagtatac
       61 tagaattgtc tttttttaat aagttttgcg ttaaattttt catagcacta gtatgatata
      121 ctagaaaaga atcaattttt attactttta aataggagaa aaaaatgaaa aaaaacaaaa
      181 accaaataat agatatcatt tttatgttat ttctaagttt tgcttacgtc atccctctat
      241 tcatgtctat gggaatctat catagtgtca atcaggatac ttactttcat ttatctcgaa
      301 tcataggact agataatgta tggagttctc cagtaaactt caataacttc gatcatcatg
      361 gaacaatgat gaatattttt tatccatggc ttactcttta tccagctttt ttattttata
      421 aaatgatggg aaatcttgtt ttaagttata atatctacta ttt
//
LOCUS       DANKKG010000376          460 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.387, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000376 DANKKG010000000
VERSION     DANKKG010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.387"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(20..91)
                     /locus_tag="PW004_003014"
     tRNA            complement(20..91)
                     /locus_tag="PW004_003014"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="PW004_003015"
     tRNA            complement(113..200)
                     /locus_tag="PW004_003015"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="PW004_003016"
     tRNA            complement(225..298)
                     /locus_tag="PW004_003016"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
     gene            complement(312..382)
                     /locus_tag="PW004_003017"
     tRNA            complement(312..382)
                     /locus_tag="PW004_003017"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:tcc)
     gene            complement(384..459)
                     /locus_tag="PW004_003018"
     tRNA            complement(384..459)
                     /locus_tag="PW004_003018"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
BASE COUNT          132 a          128 c          108 g           92 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aactacaccc gcatggcggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca
//
LOCUS       DANKKG010000377          456 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.388, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000377 DANKKG010000000
VERSION     DANKKG010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.388"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(<1..325)
                     /locus_tag="PW004_003019"
     CDS             complement(<1..325)
                     /locus_tag="PW004_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDL2727895.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKN"
BASE COUNT          152 a           73 c           44 g          187 t
ORIGIN      
        1 tatttttgta atcataatta tattgagtaa gatcaatact ctcagaaaaa gcaggttttt
       61 ctaaaaatat gtaatcaact ttttcaacaa ttacatttag tgtttccaga tgcttaaaag
      121 tcggatttgt aataaaagct atatcataat gtgtatccaa atcgtctcta ttttgaatat
      181 acgttatttc ttcaaactct ttttttccac tgctactata agctgttata tcaaaattaa
      241 tagaattctt ttttccatag cttattagat tttttagatg tctctttcca attgacccta
      301 acccaaaaaa aattactttc atcattaatt tacctcaatg tcgttaatta agttaataat
      361 atacttatct ttttcaaaag tatattgggg ttccattttc ttttccaaaa gcgtgaaaaa
      421 ttctttaatc tcctctaaat atgcatcttc aataat
//
LOCUS       DANKKG010000378          447 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.389, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000378 DANKKG010000000
VERSION     DANKKG010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.389"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..251
                     /locus_tag="PW004_003020"
     CDS             <1..251
                     /locus_tag="PW004_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727896.1"
                     /translation="DLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            261..>447
                     /locus_tag="PW004_003021"
     CDS             261..>447
                     /locus_tag="PW004_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727897.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKT"
BASE COUNT          179 a           66 c           76 g          126 t
ORIGIN      
        1 acgacttaac tgttactgaa aattatttaa tagtttctcc attacttgct cgagcagctt
       61 ctaaaacgat acgagtgaca ggaacgatga tttttgataa agttggttgg ccttctagac
      121 ctcctcaatc acggaattgg aaagaaaaaa gaaatggtta ttggtactat ggaaaattgt
      181 atttacaaag ttataaatca gctggaaaca attggattgc gacttattca ggaaccttaa
      241 ctagaaagta gggatttttt atgccagaat ttgtgaacac accaaatttt tatcaagata
      301 gcgatattct aagtaaaatt aatttaccga aagaagaatc agctttacaa gttcagcgaa
      361 ctttacagga gacacaaaaa attcaaaaat caatttctaa tctagaagat tctgaagaaa
      421 aaagaaaaaa aagagaccaa aaaacat
//
LOCUS       DANKKG010000379          424 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.390, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000379 DANKKG010000000
VERSION     DANKKG010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.390"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(<1..>424)
                     /locus_tag="PW004_003022"
     rRNA            complement(<1..>424)
                     /locus_tag="PW004_003022"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           83 a          122 c           90 g          129 t
ORIGIN      
        1 tcctctacgt ctcgtgggct cggacctcca cgaaatttta ctttcgcttc atcctgctca
       61 tgggtaggtc gcccggtttc gggtctacgt caagtaactg aacgctcagt taaaactcgc
      121 tttcgctacg gctccgcacc ttaagtgctt aaccttgcta cttaacgtaa ctcgttggcc
      181 cgttctacaa aaagtacgcg gtcacacaag aaatgtgctt ccacagcttg taagtgcagg
      241 gtttcaggtt ctatttcact cccctcccgg ggttcttttc acctttccct cacggtacta
      301 tacgctatcg gtcactaggt agtatttagc cttggagggt ggtccctcct gcttcccaca
      361 gggtttcacg tgtcccgtgg tactctggat catatctaaa gtcttctcgt ttaacataca
      421 ggac
//
LOCUS       DANKKG010000380          411 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.391, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000380 DANKKG010000000
VERSION     DANKKG010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.391"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..>411
                     /locus_tag="PW004_003023"
     CDS             <1..>411
                     /locus_tag="PW004_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDL2727898.1"
                     /translation="LIESVIENFASKIESKNLHVEIQGDNINISADKDKMNQVVINLL
                     NNAIKFTQKEGTIKFSISKNKDHVLLIVEDNGIGIEKDQQLHIFDRFYMADPSRSRAL
                     GGQGIGLAIVKSVIEAHKGSITVKSKLGLGTKFVI"
BASE COUNT          154 a           54 c           79 g          124 t
ORIGIN      
        1 ttgattgaat ccgttattga aaatttcgcc agcaaaattg agtcaaaaaa ccttcatgtt
       61 gaaatccaag gggataacat caatatatct gcagataaag ataaaatgaa ccaagttgtt
      121 atcaatcttt tgaataatgc tattaaattt acgcaaaagg agggaaccat taagttttct
      181 ataagtaaaa ataaagacca tgttctttta attgttgaag acaacggcat cggtatagaa
      241 aaagatcagc aacttcatat ttttgataga ttttatatgg ctgacccctc cagaagtaga
      301 gctttagggg ggcaaggtat cggattagct attgtaaaga gcgttattga agcgcataaa
      361 ggtagtataa ctgtaaaaag taaattaggt ctaggaacta aatttgttat a
//
LOCUS       DANKKG010000381          408 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.392, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000381 DANKKG010000000
VERSION     DANKKG010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.392"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..>408
                     /locus_tag="PW004_003024"
     CDS             <1..>408
                     /locus_tag="PW004_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HDL2727899.1"
                     /translation="QDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHTTIYRWVQEY
                     GKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWLRKKRDTQA
                     AYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKL"
BASE COUNT          138 a           67 c           83 g          120 t
ORIGIN      
        1 gcaggatgtg attattgtag ccgtgggcta ctatcttcgt tataacctta gctatcgtga
       61 agttcaagaa atcttatatg atcgtggcat taacgtttct catacgacga tttatcgttg
      121 ggtgcaagaa tatggcaaac tactctatca aatttggaaa aagaaaaata aaaaatcctt
      181 ttattcatgg aaaatggatg aaacgtacat caaaattaaa ggaaaatggc attatttgta
      241 tcgagccatc gatgcagatg gtttaacctt ggatatttgg ttacgtaaaa aacgggacac
      301 acaagcagcc tatgcttttc ttaagcggtt agtgaagcag tttgatgaac cgaaggttgt
      361 agtcacagat aaagccccct ctattacaag tgcctttaag aaactaaa
//
LOCUS       DANKKG010000382          400 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.393, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000382 DANKKG010000000
VERSION     DANKKG010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.393"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..>400
                     /locus_tag="PW004_003025"
     CDS             <1..>400
                     /locus_tag="PW004_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2727900.1"
                     /translation="YRWFLGYSFEEKIPHFSTFGKNYVRRFRETTVFEDIFAYILEQA
                     VKAGFVTEDNLYLDSTHIKANANKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRP
                     FTLDLEKEVKLKERKISKADPESGYYVKGER"
BASE COUNT          142 a           69 c           89 g          100 t
ORIGIN      
        1 taccgctggt ttttaggcta ctccttcgag gagaaaatcc cccatttctc aacttttgga
       61 aagaattatg ttcgccgatt tagagaaacg acggtgtttg aggatatttt tgcctatatt
      121 ttagaacaag cagttaaagc aggttttgta acagaggata atttgtatct ggattctacc
      181 cacatcaaag ctaacgccaa taaacataag tttacgaaag aaatgaccca tgatgaagcg
      241 aaagcctatc aggatgaatt agaagatgag atcaatcgtc aacgaataga ggctggaaaa
      301 cgccctttta ctttggactt agaaaaggaa gtgaagctga aagaaagaaa aataagtaaa
      361 gctgatccgg aaagcggcta ctacgtcaaa ggcgaacgag
//
LOCUS       DANKKG010000383          399 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.394, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000383 DANKKG010000000
VERSION     DANKKG010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.394"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..358
                     /locus_tag="PW004_003026"
     CDS             <1..358
                     /locus_tag="PW004_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL2727901.1"
                     /translation="KAVELDIEKRVKFLGERNDIPELLSVMDVFVMPSLYEGLPMVGL
                     EAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYANKITQELKSYD
                     VSYTTEQMESIYSRDH"
BASE COUNT          163 a           44 c           76 g          116 t
ORIGIN      
        1 caaagcagta gaattggata tagagaaaag agtaaaattt ttaggagaaa gaaacgatat
       61 tcccgagtta ttatctgtga tggatgtttt tgtaatgcct tctctttatg aaggtctgcc
      121 aatggttgga ttagaagcac aagcagcaaa attaaaatta gtcttagcag atactatttc
      181 caaagataca aagttaacaa ataatgtgga ttttgtatca ttagatgaaa caccacaaga
      241 atgggcaaaa aaagtaataa aagagccgta tgctaataaa ataacacaag aactaaagag
      301 ttatgatgtt tcgtacacta ctgaacaaat ggagagtata tatagtagag atcattaaaa
      361 atgttcaaga tattttagga gtgattattt ttgaaaata
//
LOCUS       DANKKG010000384          398 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.395, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000384 DANKKG010000000
VERSION     DANKKG010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.395"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            complement(83..244)
                     /locus_tag="PW004_003027"
     CDS             complement(83..244)
                     /locus_tag="PW004_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDL2727902.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
     gene            314..>398
                     /locus_tag="PW004_003028"
     CDS             314..>398
                     /locus_tag="PW004_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2727903.1"
                     /translation="MPRRQRRTYSKEFKQQIVDLYLAGKPCA"
BASE COUNT          130 a           85 c           47 g          136 t
ORIGIN      
        1 tgataaaaat tattaaagtt ctatttttct ttcttgtaat atatgctata gcatttttta
       61 tcactttcct aactactcct ttctattttc ttactccata actaattgca tccactggac
      121 aagcattttt acatcgtcca catctgatac actccagatg attgcaattc tcaactgaat
      181 ctttattcat ttggcaaact tttttacatt ttccacaatt aatacactta tccttatcct
      241 gaatcggcta cactagacta gacagaaaaa ataaggtgtg tagaataata aaaaacacac
      301 tggaggattt ttcatgccaa gacgtcaacg aagaacctat tcaaaagaat tcaaacaaca
      361 aatcgtcgat ctctatctcg ctggtaagcc ttgcgcag
//
LOCUS       DANKKG010000385          393 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.396, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000385 DANKKG010000000
VERSION     DANKKG010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.396"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            200..>393
                     /locus_tag="PW004_003029"
     CDS             200..>393
                     /locus_tag="PW004_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727904.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEG"
BASE COUNT          153 a           50 c           71 g          119 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg cat
//
LOCUS       DANKKG010000386          393 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.397, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000386 DANKKG010000000
VERSION     DANKKG010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.397"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..371
                     /locus_tag="PW004_003030"
     CDS             <1..371
                     /locus_tag="PW004_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727905.1"
                     /translation="KKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTLLI
                     SPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLSEE
                     KILLPENWAELRKLVEYGII"
BASE COUNT          148 a           60 c           56 g          129 t
ORIGIN      
        1 gaaaaaaaga aactttaatt aagcttcatc ctagagcaag gacaaatcgt tatggtgaaa
       61 aaaacatatt gaattccagt ttaccgtttg aagtcttgct acttgaaaat gacttctctt
      121 caacactttt gattagtcct atatctacaa tttctttttc accatctatg atgtttgaaa
      181 agacgatcaa ctctattatg ttggctaaat taattttaaa tgaatataca gcattatctg
      241 aagataaaaa agaaacattg acaaatattg ttcttttctg tgaacattat aaccgtctat
      301 cagaagaaaa aattttactc ccagaaaact gggcagaatt aagaaagttg gtagagtatg
      361 ggattattta acacactttc caagtcaaaa tta
//
LOCUS       DANKKG010000387          387 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.398, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000387 DANKKG010000000
VERSION     DANKKG010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.398"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..>387
                     /locus_tag="PW004_003031"
     CDS             <1..>387
                     /locus_tag="PW004_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727906.1"
                     /translation="FLALILHLSGVSNSIHANKDSLTLIAPEDAVAIAENYNHTDYAN
                     KESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIELDNSNNKNTVLTTPIYRYKGQV
                     ASINGKLVQTKLSKFGTTELTIPPGI"
BASE COUNT          147 a           70 c           52 g          118 t
ORIGIN      
        1 cgtttcttgc actaatattg catctctcag gagtaagcaa ttctatccat gctaacaaag
       61 attcattaac acttatagca cctgaagacg ctgttgctat agctgaaaat tacaatcata
      121 ctgattatgc aaacaaagag tcaatctatc atcctgatat gattaataat gatcaatatt
      181 tactaggtaa ccaggaaata aatccaacaa ctcattttat gagtaacaaa cttacaattg
      241 aattagataa ttcgaataat aaaaatactg tcttgacgac acctatttat cgttataaag
      301 ggcaagtggc ttctattaat ggcaaactag tacaaacaaa attatctaag tttggcacaa
      361 ctgaattaac tattcctcca ggtataa
//
LOCUS       DANKKG010000388          384 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.399, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000388 DANKKG010000000
VERSION     DANKKG010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.399"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..>384
                     /locus_tag="PW004_003032"
     CDS             <1..>384
                     /locus_tag="PW004_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2727907.1"
                     /translation="ANSKEKVLSGVNQKFELGKFYAIVGKSGTGKSTLLSLLAGLDKP
                     QTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIRLVNKSADESILFE
                     LGLDKKQIKRNVMKLSGGQQQRVAIA"
BASE COUNT          167 a           40 c           62 g          115 t
ORIGIN      
        1 gcaaattcta aagaaaaagt tttgtcagga gtaaatcaaa aatttgaact tgggaagttt
       61 tatgcgatag taggaaagtc aggaacagga aaatctacac ttctttcctt acttgctgga
      121 cttgataaac ctcaaacagg aaaaatattg tttaagaatg aagatataca aaataaagga
      181 tatagtaatc atagaaaaaa taatatatct ttagtatttc aaaactataa tttaatagat
      241 tatttatcac caattgaaaa tattagatta gtaaataaat cagcagatga aagtatcttg
      301 tttgaactag gtttagataa aaaacaaata aaaagaaatg ttatgaaatt atctggtgga
      361 cagcaacaaa gagtagctat tgct
//
LOCUS       DANKKG010000389          383 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.400, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000389 DANKKG010000000
VERSION     DANKKG010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..383
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.400"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..>383
                     /locus_tag="PW004_003033"
     CDS             <1..>383
                     /locus_tag="PW004_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL2727908.1"
                     /translation="QYHVGQYLPDWHPWEDYRNFFVSNKKTNGCREIFAIELPWIIDT
                     FGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETGIVGTLSVDVVSRVAKRNLTIIGEK
                     ILIEWEGNPDSLFSWNEETSSMTDI"
BASE COUNT          131 a           50 c           73 g          129 t
ORIGIN      
        1 caataccatg taggacagta tttacccgac tggcatcctt gggaagatta tcgcaatttt
       61 tttgtttcaa acaaaaaaac taatgggtgt agagaaatat tcgctattga attaccttgg
      121 attatagata catttgggaa agttaaatcg tttactgtaa tgacccagaa attatccaat
      181 cttgatattg attattcgga cagtttcact cttattattg agcatgaaac tggtatagtt
      241 ggaactttaa gtgtagatgt tgtttcaaga gtagctaaaa gaaatttaac tataattggt
      301 gaaaagattt taatagaatg ggaaggtaac ccggattctc tattttcatg gaatgaagaa
      361 acttctagta tgacagatat tca
//
LOCUS       DANKKG010000390          382 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.401, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000390 DANKKG010000000
VERSION     DANKKG010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.401"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..>382
                     /locus_tag="PW004_003034"
     CDS             <1..>382
                     /locus_tag="PW004_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2727909.1"
                     /translation="GLQYDSLSEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYM
                     SDFNDYVSSMITGQGSAGGYVVLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSK
                     KGWRGPFNSAWTLDSIFNANFIQTG"
BASE COUNT          141 a           48 c           74 g          119 t
ORIGIN      
        1 ggactacaat acgattcttt atctgaaaaa actttgaaat tagaagcagg agacattcaa
       61 gttgcttttt atcagcaaca agctgtaact attcaggata aattaaaaga ttatgcaacg
      121 aataattata tgagtgattt taatgattat gtttcttcaa tgattacagg tcaaggcagt
      181 gcaggaggat atgtagtttt atggccaaaa gaaaaacctt ccaacatctt tattatggat
      241 aatccagatt tagaaaaggc aagagaagta ctaagaatga ataaaaatgg gattgctttt
      301 tctaagaaag gatggcgggg gccttttaat tcagcttgga cattagatag tatatttaat
      361 gccaatttta tacaaactgg at
//
LOCUS       DANKKG010000391          345 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.denovo.403, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000391 DANKKG010000000
VERSION     DANKKG010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.denovo.403"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
BASE COUNT          117 a           44 c           38 g          146 t
ORIGIN      
        1 ttacctttaa attatactac caacaaatgg tattttatta ttaatttata taatatgaaa
       61 cctaaaatat agtagtatgt taatttatac taaattaaat aaagtactac ctttaatagg
      121 tagtacttta ttatcaagcc attattcctt cttctactag ttctttttgt cacataattt
      181 tgtaatcaat aaatgttgcg caataagtat aagaattcca ttttctaaat ttgaaaggtg
      241 atccataatt tcatgttcaa gtagtttatt aattgtccac ttgtcgcttc atctaaaaat
      301 tttataggtt ttaggaatgc tattcgctta ttttaggaag atttt
//
LOCUS       DANKKG010000392         4402 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.guided.159, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000392 DANKKG010000000
VERSION     DANKKG010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.guided.159"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..187
                     /locus_tag="PW004_003035"
     CDS             <1..187
                     /locus_tag="PW004_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HDL2727910.1"
                     /translation="ETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVS
                     EINNFLNEESSDERKQI"
     gene            168..902
                     /locus_tag="PW004_003036"
     CDS             168..902
                     /locus_tag="PW004_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL2727911.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            935..1843
                     /locus_tag="PW004_003037"
     CDS             935..1843
                     /locus_tag="PW004_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HDL2727912.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            1840..2382
                     /gene="sat4"
                     /locus_tag="PW004_003038"
     CDS             1840..2382
                     /gene="sat4"
                     /locus_tag="PW004_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HDL2727913.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            2475..3269
                     /locus_tag="PW004_003039"
                     /note="aph(3')-IIIa"
     CDS             2475..3269
                     /locus_tag="PW004_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HDL2727914.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            3545..4117
                     /locus_tag="PW004_003040"
     CDS             3545..4117
                     /locus_tag="PW004_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HDL2727915.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIEPISLGFYEQNWYLIGFAG"
BASE COUNT         1517 a          667 c          994 g         1224 t
ORIGIN      
        1 tgaaactttt gaatataaac ctgaaaaata tgccgaaaga gttaatcata tttttgaagt
       61 actcggtctt tctctttttg aatgctacga catgaccgag aaactttata aagaagtgaa
      121 tgaaattgta tcggagataa ataacttttt aaacgaggag agttcagatg aaagaaaaca
      181 aatatgatga taatatattt tttcaaaaat acagtcaaat gagtcgctcg cagaaaggac
      241 tggctggtgc gggagaatgg gagactttga aaaagatgct acctgatttt aagggtaagc
      301 gtgtgcttga tttaggatgc ggctatggat ggcactgtat atatgcgatg gaaaacggtg
      361 cttcctctgt agtaggtgtt gatatttctc ataaaatgct cgaagtagca aaaggaaaaa
      421 cccattttcc acagattgaa tatgaatgct gtgccataga agatgtggat ttcccagagg
      481 agagctttga tgtaatacta agttcgcttg cgtttcatta tgtagcagac tatgagaatt
      541 taataaaaaa gatatatagg atgctgaagg ctggtggcaa tttagttttt acagttgaac
      601 atcctgtttt tactgctcat ggaacacaag actggtatta taacgaaaaa ggagaaatac
      661 tgcatttccc ggtggacaat tattattatg agggcaaacg gacagctatg tttttggaag
      721 aaaaggttac aaaatatcat agaacactga ccacatatct aaatacactg ctttcaaata
      781 gttttataat aaatcagatt gtggagccac agccgccaga gaacatgatg gatattccgg
      841 ggatggcgga tgaaatgcga cgcccaatga tgctgattgt atcggcaaaa aagaagatgt
      901 aataatatag aaaaaataaa cgaggagtat gtaaatgaga tcagaaaaag aaatgatgga
      961 tttagtactt tctttagcag aacaggatga acgtattcga attgtgaccc ttgaggggtc
     1021 acgcgcaaat attaatatac ctaaagatga atttcaggat tatgatatta catattttgt
     1081 aagtgatata gaaccgttta tatctaatga tgactggctt aatcaatttg ggaatataat
     1141 aatgatgcaa aagccggagg atatggaatt attcccacct gaagaaaagg gattttccta
     1201 tcttatgcta tttgatgatt acaataaaat tgatcttacc ttattgccct tggaagagtt
     1261 agataattac ctaaagggcg ataaattaat aaaggttcta attgataaag attgtagaat
     1321 taaaagggac atagttccga ctgatataga ttatcatgta agaaagccaa gcgcaaggga
     1381 gtatgatgat tgctgcaatg aattttggaa tgtaacacct tatgttatta aaggattgtg
     1441 ccgtaaggaa attttatttg ctattgatca ttttaatcag attgttcgcc atgagctgct
     1501 gagaatgata tcatggaagg tcggcatcga aacaggcttt aaattaagtg taggcaagaa
     1561 ctataagttt attgaaaggt atatatccga ggatttgtgg gagaaacttt tgtccaccta
     1621 ccggatggat tcctatgaaa acatatggga agcattattt ctatgccatc aattgttcag
     1681 ggcggtatcc ggtgaggtgg cggaaaggct tcattatgcc tatccggagt atgataggaa
     1741 tataacaaaa tataccaggg acatgtataa aaaatacact ggtaaaaccg gctgcctgga
     1801 tagcacatat gccgctgata tagaagagag gcgggaacag tgattacaga aatgaaagca
     1861 gggcacctga aagatatcga taaacccagc gaaccatttg aggtgatagg taagattata
     1921 ccgaggtatg aaaacgagaa ttggaccttt acagaattac tctatgaagc gccatattta
     1981 aaaagctacc aagacgaaga ggatgaagag gatgaggagg cagattgcct tgaatatatt
     2041 gacaatactg ataagataat atatctttac taccaagacg ataaatgcgt cggaaaagtt
     2101 aaactgcgaa aaaattggaa ccggtacgct tatatagaag atatcgccgt atgtaaggat
     2161 ttcagggggc aaggcatagg cagcgcgctt atcaatatat ctatagaatg ggcaaagcat
     2221 aaaaacttgc atggactaat gcttgaaacc caggacaata accttatagc ttgtaaattc
     2281 tatcataatt gtggtttcaa aatcggctcc gtcgatacta tgttatacgc caactttgaa
     2341 aacaactttg aaaaagctgt tttctggtat ttaaggtttt agaatgcaag gaacagtgaa
     2401 ttggagttcg tcttgttata attagcttct tggggtatct ttaaatactg tagaaaagag
     2461 gaaggaaata ataaatggct aaaatgagaa tatcaccgga attgaaaaaa ctgatcgaaa
     2521 aataccgctg cgtaaaagat acggaaggaa tgtctcctgc taaggtatat aagctggtgg
     2581 gagaaaatga aaacctatat ttaaaaatga cggacagccg gtataaaggg accacctatg
     2641 atgtggaacg ggaaaaggac atgatgctat ggctggaagg aaagctgcct gttccaaagg
     2701 tcctgcactt tgaacggcat gatggctgga gcaatctgct catgagtgag gccgatggcg
     2761 tcctttgctc ggaagagtat gaagatgaac aaagccctga aaagattatc gagctgtatg
     2821 cggagtgcat caggctcttt cactccatcg acatatcgga ttgtccctat acgaatagct
     2881 tagacagccg cttagccgaa ttggattact tactgaataa cgatctggcc gatgtggatt
     2941 gcgaaaactg ggaagaagac actccattta aagatccgcg cgagctgtat gattttttaa
     3001 agacggaaaa gcccgaagag gaacttgtct tttcccacgg cgacctggga gacagcaaca
     3061 tctttgtgaa agatggcaaa gtaagtggct ttattgatct tgggagaagc ggcagggcgg
     3121 acaagtggta tgacattgcc ttctgcgtcc ggtcgatcag ggaggatatc ggggaagaac
     3181 agtatgtcga gctatttttt gacttactgg ggatcaagcc tgattgggag aaaataaaat
     3241 attatatttt actggatgaa ttgttttagt acctagattt agatgtctaa aaagctttaa
     3301 ctacaagctt tttagacatc taatcttttc tgaagtacat ccgcaactgt ccatactctg
     3361 atgttttata tcttttctaa aagttcgcta gatagagttc tatattagag atgtaaagag
     3421 ttttggtgaa gaggtttgtg agtatggctt ttataaccag ggtgcaatga gaagcactaa
     3481 cagcagctag cttaagaacg cataggatat actttttatg gaagtcacag aggaggaata
     3541 aaatttgaac aggattaaca gagtaacctc tattcttatt cagctgcaat caaaaaaaat
     3601 aattcctgcc aaagaaattg cacagcgatt taatataagc ttaaggacag tttacagaga
     3661 tatccggaca cttgaagaag ccggaatacc aattggatct gaggccggaa aaggatattt
     3721 tcttgtagag ggcttcctac ttccgccggt aatgtttaca gcggcggaag tgggtgcatt
     3781 gattacagca gggaaatttt taaattgcca tggagatgaa tcatttataa aggattttga
     3841 ttcagctatg tataaaatca aatctatttt gaagcatggt gaaaaaaact atgcacagga
     3901 gttggagaac agtattaacg tgtacagcac atctggacag aaaaatacat tggcggataa
     3961 cgttattgca gcaattcaga ctgcaatctg caataaaagg gtaatatcaa ttcaatatcc
     4021 tgcatcagga gggcaagaac cggaaagcag aatgatagag cctatatcac tggggtttta
     4081 tgaacagaac tggtacttaa tcggttttgc aggctaagaa atgaatatag gaattttcgt
     4141 gtggatcgga ttaaaagcat ttgcatagaa gaagagctct gtcaaagcca tgatggctct
     4201 ttggaacaaa tattaaaaca aatgctttca tataaaaaac tatatgccct acaatgccgt
     4261 tgtaaaaatt ggggaaaagg gatatattga ttttatatct ttctcaaaat aaatataaaa
     4321 gccctttaaa gagggctttt atatattaat cacaaatcac ttatcacaaa tcacaagtga
     4381 ttaatcacaa atcacttgtg at
//
LOCUS       DANKKG010000393         3945 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.guided.174, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000393 DANKKG010000000
VERSION     DANKKG010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.guided.174"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            159..1280
                     /locus_tag="PW004_003041"
     CDS             159..1280
                     /locus_tag="PW004_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDL2727916.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            1538..3040
                     /gene="eat(A)"
                     /locus_tag="PW004_003042"
     CDS             1538..3040
                     /gene="eat(A)"
                     /locus_tag="PW004_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HDL2727917.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            3068..3634
                     /locus_tag="PW004_003043"
     CDS             3068..3634
                     /locus_tag="PW004_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDL2727918.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            3845..>3945
                     /locus_tag="PW004_003044"
     CDS             3845..>3945
                     /locus_tag="PW004_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 family protein"
                     /protein_id="HDL2727919.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTS"
BASE COUNT         1337 a          670 c          863 g         1075 t
ORIGIN      
        1 cattgctccg gccccagcta ttgcgatttt catgtttttt ctctccttta ttcaatgagt
       61 tcccttttag tatatagaaa aatggaagaa aatagcattg ttttgtttga aaaaaatgaa
      121 aaaaagtgtc ttacttaaga agaaagtgag ggatacagat ggatattttg gcggcaattg
      181 attcatttaa aggaagtgct tcttcatttg aactaaatca ggcagttcta aatggctggg
      241 aagatcgtaa aggaagaaaa atcaatgtgc cgattgcaga tggcggagaa aatactgcag
      301 aagcaatcta ttacgcgctc aggggaacat ggcgtaccat tgatgggatg gatctcttat
      361 tccgtcagcg aaaaatcagc tatttattga caacttggca aggaaaaaac accgcagtca
      421 tcgaatctgc acaagcattg gggttagatc tacttgataa accaacggat caaactatcc
      481 gacttgcttc cagttatgcc ttaggagaac ttattaaaga tgcactgctt caagaagtag
      541 aacaaattat tgtcacttta ggaggaagcg ggtgtagcga tggtggtcta ggattactcc
      601 aatccttggg tgcttcttta gaagggtaca cagaaggaaa tcctttatta tctactaaaa
      661 aacttcattt tgatcgaaga aacgttcttg tttctaaagt aaaattgatt atcgctgcag
      721 atgttaccaa tccatataca ggtcaacaag gagctgcttg gcaatttgga agacaaaaag
      781 gaggaataga tacgacgcta gctttcttag ataaacaagc aggaaagatt gctcagcaaa
      841 taaaagattt ctgggaggtg gatttagatg atattccagg aagtggagca gcaggcggta
      901 ttggtggagc cctttttttg ttgggagcga agatggtttc tggtttcgat ttagtcagcc
      961 aaatcactgg attggaaaaa aagatcgaac aagcagatgt catcattaca ggagaagggc
     1021 ggatcgatca gcaaacgatc cacggaaaag ttccatacgg tgtagcagtg cttgcaaaaa
     1081 aatatcgaaa accaataatc gctatttgcg gaagtagaga ccaagagcta gaaggacttt
     1141 ctgattttct cccaattgtc ttaagtattc atttgggtcc aataggttta gaagaagcaa
     1201 tggaacacag gcaaacatta gaaaaaacaa aaatcttagg acagacactt agcagatttt
     1261 tcaccatcct cccgaagtag ttgacaaaat cattttcttt atgctatcgt aatttcaaat
     1321 ataggacaaa gtgagggatc cgtgcattga aaaattaagt cgattgatga aagctgcatt
     1381 cagactgtac cttgtggaga caaggaagca gtgtgcagat tttcgggatt ggcttggttt
     1441 tttcgatgta tgggtctttt tgtgtgtatc caacccttgc atcctcttta gccagttctg
     1501 aaaataggac tggtcttttt ttacgaggtg atggaaaatg tctaaaatcg aaataaaaaa
     1561 tctgacattc ggctacgaca gccaaggcac attattattt gaacaagcaa atctaaattt
     1621 tgacacacaa tggaaactag gacttatcgg acgaaacggt cgaggaaaga caactttact
     1681 gaatattcta caaaacaaac taccttatca agggcaagta atccatcagc aagaatttgc
     1741 ctatttcccg caacagacaa aagataaaga acgtttaacc tattacgtgt taaatgatat
     1801 tacggatttt gagatatggg aaatcgaaag agagctccaa ttgatgcaaa cagatcctga
     1861 aatcttatgg agagaattca gcacactatc ggggggagag aagacaaaag tcctactggc
     1921 acttttattt gtggatgaca ctcatttccc gttaatcgat gaaccaacga atcatttgga
     1981 tatctctggt agaaaacaag tagcggctta tttgaaaaag aaaaaacaag gcttcatcgt
     2041 ggtcagccat gaccggggat ttatcgatga agtagtggac catgttttag caatcgaaaa
     2101 aagtcaactg gaactttatc aagggaattt ctctatctat gaagaacaga aaaaacttcg
     2161 tgatgaattt gaaatggctc aaaatgaaaa attgaaaaaa gaagtcagta ggctaaagaa
     2221 aacagcagct gaaaaagccg aatggtctcg ttcccgagaa ggagataaaa caaagaaaca
     2281 agtcggattc atcgatactg aatctagacg agtgaataaa ggagcagtgg gtgctgatgc
     2341 tgcacggacg atgaaacgat ccaaagcaat cgtgaatcgg atggagaccc agatcagcga
     2401 gaaagaaaaa ctattaaaag atatcgaata tatcgattct ttgacgatga atagccaagc
     2461 gtctcaccat aagcgacttt taagcgtaga agatcttcaa ttagggtatg aaaatctgtt
     2521 attcgagcca attcatttta caatcgagcc tcatcagcgg gtggcgattt caggtcctaa
     2581 cggtgcagga aagtcatcca ttatccatta tcttctgggg gcattcaacg gcaaggttat
     2641 aggagaaaaa taccagccaa aacatctgag cattagttat gcaagccaaa attatgaaga
     2701 caatcgagga acgttggcgg aatttgcaga gaaaaaccaa gtagactacc aagcattttt
     2761 gaacaacctc cgaaagcttg ggatggaaag agatgttttt cataacaaga tcgagcagat
     2821 gagtatgggc caacggaaaa aagtggaatt ggctaaatct ttatcacagc cagctgaact
     2881 atatacatgg gatgaaccat tgaattattt ggatgtcttc aatcaagaac aattagaaca
     2941 actgatcttg aacgtgaaac ctgccatgtt actagtggaa catgatcaaa ccttcctgga
     3001 taaagtatct actgagatta tttctcttga gagaatctaa cccttgtaat ctcaagaaca
     3061 acaaggtatg gttaagggga actttgccaa cgaaggagag aatcacatgc tttatgtaac
     3121 gagacacgga gaaacaactt ggaacgcaca aggattagtt tgcggacggg cagatgtgcc
     3181 gctgactgaa aaaggacaga tgcaggcaca gaaactagca gaaaaggtag tagatttgcc
     3241 tgttccaatc acgaaaatca tccattcccc tcttcaacga gcaagagata cagcacaagc
     3301 agttgctgat cgtctcagct tgccgctgac cgttgatgaa cgattggtag aaatggattt
     3361 tggggattat gatggactgc caagcaaaga tgagaacttt caaaaagcac gtttagcgtt
     3421 cgctgtacga ttcccaaatg gggagtcagt cttagatgtg tatgcacgga tcgttccttt
     3481 attgaaagaa tgcatcgaag acgaagaaaa tgtctatctg ttagtttgcc ataatgcctt
     3541 gataagagtg atcaatgctt attttcatcc gatgcctaat gaggggttct ttacattcat
     3601 ggtagataat acagaattgg tttcttatga gtaattatgt ttttgtgtta tttaaataaa
     3661 atatttcaag gcactgcttt tgcttagcag tgcctttttt aggttatgac aattcttttg
     3721 ataatataaa ctcaaccttt agaaatgaaa tttaattatt ttaataaaag tattgaaatt
     3781 ttgtttcttt agatatatac tctagtcata gaaatgaaga cggatacaga aaaagaggga
     3841 taatatgttt ggcttttcaa tatttatgaa cgattctttg tcagaagaga agaaacagta
     3901 tatccgccaa atggcatcca atggctttgt cggtattttc acttc
//
LOCUS       DANKKG010000394         2458 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.guided.223, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000394 DANKKG010000000
VERSION     DANKKG010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.guided.223"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            5..742
                     /gene="erm(B)"
                     /locus_tag="PW004_003045"
     CDS             5..742
                     /gene="erm(B)"
                     /locus_tag="PW004_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HDL2727920.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1005..1868)
                     /locus_tag="PW004_003046"
     CDS             complement(1005..1868)
                     /locus_tag="PW004_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HDL2727921.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(1870..2142)
                     /locus_tag="PW004_003047"
     CDS             complement(1870..2142)
                     /locus_tag="PW004_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDL2727922.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2159..2368)
                     /locus_tag="PW004_003048"
     CDS             complement(2159..2368)
                     /locus_tag="PW004_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HDL2727923.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
BASE COUNT          728 a          449 c          395 g          886 t
ORIGIN      
        1 ttacatgaac aaaaatataa aatattctca aaacttttta acgagtgaaa aagtactcaa
       61 ccaaataata aaacaattga atttaaaaga aaccgatacc gtttacgaaa ttggaacagg
      121 taaagggcat ttaacgacga aactggctaa aataagtaaa caggtaacgt ctattgaatt
      181 agacagtcat ctattcaact tatcgtcaga aaaattaaaa ctgaacactc gtgtcacttt
      241 aattcaccaa gatattctac agtttcaatt ccctaacaaa cagaggtata aaattgttgg
      301 gaatattcct taccatttaa gcacacaaat tattaaaaaa gtggtttttg aaagccatgc
      361 gtctgacatc tatctgattg ttgaagaagg attctacaag cgtaccttgg atattcaccg
      421 aacactaggg ttgctcttgc acactcaagt ctcgattcag caattgctta agctgccagc
      481 ggaatgcttt catcctaaac caaaagtaaa cagtgtctta ataaaactta cccgccatac
      541 cacagatgtt ccagataaat attggaagct atatacgtac tttgtttcaa aatgggtcaa
      601 tcgagaatat cgtcaactgt ttactaaaaa tcagtttcat caagcaatga aacacgccaa
      661 agtaaacaat ttaagtaccg ttacttatga gcaagtattg tctattttta atagttatct
      721 attatttaac gggaggaaat aattctatga gtcgcttttg taaatttgga aagttacacg
      781 ttactaaagg gaatgtagat aaattattag gtatagggca cctctaataa ctaccaatta
      841 attcacctca aaatgaaatt agaaaaaaac agcgaaattt ttaaatctat ttcttatcga
      901 tacaaattcc tcgtaggcgc tcgggacccc aacatgagga aatgttttta taaaaaaaga
      961 gtagttaccg ttttttggta actactcttt tcctttttta aggtttaaat acctggaagt
     1021 ttaggtgttt tgggtattgg tggtgtaggt agctgcaagc tttccatttt ttgttgaatt
     1081 gctttaaatt caggggtttc ttggtgttga ttttgaacca ttttttgctc tattctctcc
     1141 aaagtcggtt gaatttcttt ccctgatact ttacgattta attctctttc taaggtctct
     1201 ttcggactaa tggaaggcgt ttctaagctt gaatagagtt tcactccttc tctattgtaa
     1261 agccttatat cgctaaataa gcccgtttta tgaagggttt ctaaattggt cggtaagttt
     1321 ttgacaacaa tatcatgcgc ttgttttggt gttgccctag ctgtcattgg atcatctgca
     1381 tacatggttt catatcgttc aatcgttcct aagtatgatt caattttcgg tactgccatg
     1441 acgtatattt ttgtttcata acctttagat tgaagcattg tggctgtttg aatgggaacg
     1501 tctgttgttc gtcctgttcc ttcaatcact aaattatagc cttgatcgct taaacggcta
     1561 ataagtgctt ctgtcatgcg attcgaataa ggggtaacgt gttttactac gtctttttca
     1621 tagagtttca ctaattcgtc aaaatttggg tgctgttgtt taaaggtatc attatcaatg
     1681 acaataacat tcccttgtgt ttcttcaaaa attgctgatc gcaaactggt tttccctgac
     1741 cctggttgcc caccaagtaa aaaagcggtt ggcgattcaa ccgctagtct atttttagtt
     1801 agtcgttcaa cattcttttc taaacgatct tcaaattgct tttcggtgaa gtcagtaata
     1861 ttcgccatgt taagccactt tctctttatt caaaatttgt tttgaatatt gattcattga
     1921 ccgccaatac tcatgaatag cttgtaattc ttcaaatggt tgttgaaata aatcaacttc
     1981 ttgtgccact tctaattgat ttaaaagatt cacttctagt aaacgagtat ttttagtatc
     2041 tagttcatga ttcaaaacat aatttaatac ccgattataa acacttgccg ataactcatt
     2101 aatgatttca atttcaaatg ttttttcata cgtaactgcc atttcatttt cactcctttt
     2161 aaagtttgtc aggtaaatat ttccgtatat attcttctaa ggcttgatcc ataacttctt
     2221 ttacgtttcc gccattcttt gctgtttcga tttttatgat atggtgcaag tcggcacgaa
     2281 cacgaaccgt cttatccccc attatatctt tttttgcgct gattggcgta tcatttcgtt
     2341 ttgctttctg tgcgcctaaa tttcccacga tcgctcactt ctttctattt cttcttacta
     2401 tttattttat catcacctat cacaaatcac aagtgatttg tgattaatca cttgtgat
//
LOCUS       DANKKG010000395         2387 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.guided.225, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000395 DANKKG010000000
VERSION     DANKKG010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.guided.225"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..93
                     /locus_tag="PW004_003049"
     CDS             <1..93
                     /locus_tag="PW004_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDL2727924.1"
                     /translation="VPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            447..1925
                     /gene="msr(C)"
                     /locus_tag="PW004_003050"
     CDS             447..1925
                     /gene="msr(C)"
                     /locus_tag="PW004_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HDL2727925.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            1972..>2387
                     /locus_tag="PW004_003051"
     CDS             1972..>2387
                     /locus_tag="PW004_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HDL2727926.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSR"
BASE COUNT          804 a          386 c          509 g          688 t
ORIGIN      
        1 gttccagtta ttattttatc aataggaaaa gcggtagaag aaggttacga gtctgttcgt
       61 ttagcaccag aaaagatcac tacgttcgaa taaaaggttt tcagttacta ttcagtagct
      121 attgcttttt ttgaatcgtg tgtggtaacc taataaaaaa ggaggtcgga aaaatgactg
      181 catcgatgaa attacgtttc gaacttttga ataacaacta aataaattca aaagacatgt
      241 ataaacaaca atttcatgga tggagtatgt ccattttttc gaacgacagt agaataggtg
      301 aaagctatga cagatttggt gatttagtca tagttatttc gttcagagta ggaaaagatg
      361 tgatgcagtt tttttgcaaa cagatttttc tgctcttttt tagtatcgat aattgttgtt
      421 ttacatgaaa taggagtgta aaacatatgg aaaatttagc agtaaatata acaaatctac
      481 aagtcagctt tggcaaccag ctagaactat ctattgattc tcttcgtgtc tatcagcaag
      541 atcgtatagg gatcatagga gaaaatggtg tcggtaaatc gactttgctc aaactaatag
      601 ccggtgaact ttttcccgat catgggaaaa tccaaacaga gatcaccttc aactacctgc
      661 ctcaattaac ctatctcgct gaagcaaagg acctaaattt ggaattagcc agtcatttcc
      721 agttaagact ggaagaaact tcagagcgga aatggagcgg aggggaagaa cgaaagatcg
      781 agttgatacg tcttctttct tcttatgaac aagggatgct tctagacgag ccaacaaccc
      841 atctagatag aaaaagtatt gatcgactga ttgaagagct tcgttattat tatggtacgc
      901 tggtttttgt tagtcatgat cgctattttc tagatgagct agcatcgaaa atctgggaag
      961 taaaagacgg agaaatccga gagttttcgg ggaattatag tgcctatctc actcaaaagg
     1021 aattggagaa aaagactcag ctacgagaag cagagtcgat catgaaagag aaaaaacgat
     1081 tggaaaaatc gatccaagaa aagaaaaaac aagcggaaaa gttggaaaaa gtgtccagta
     1141 aaaagaaaaa gcaacaaatc agaccggatc ggttgtcttc ctctaaacaa aaagacagtg
     1201 tacaaaaagc catccaaaag aatgcgaaaa cattagagag aagactccaa aaaataggag
     1261 aaacaaccaa gccgcagcag atgaaacaaa tccgttttcc agtaccaaaa tctcttgaac
     1321 tccacagccg ctatcccatc atgggacaaa atgtccaatt ggaaagaagt gggagaacat
     1381 tactggtaaa tggtgatttt cagttttctt taggtaaaaa aatcgcgatt gtcggtgaaa
     1441 atggttcagg taagacaacc ttattagaac atatccgcaa acaaggagaa ggaatccttc
     1501 tctctccgaa agtaagcttt caagtatatc agcaaaaggg ttatcaaatg acatctgaag
     1561 aatccatcat tcgttttgtc atgagacaaa cagagttttc agaatcgctt gtccgtagtt
     1621 tgctgaatca cttagggttt gctcaggaaa ctttgacgaa accgttatgt acattaagtg
     1681 ggggagaagc gacccgtctg acgattgctt tgctttttac taagccaagt aatgtgttgc
     1741 tgttagatga accgactaat tttattgata tggcaacgat cgaagcttta gagaagctaa
     1801 tgcaaatata tccgggaacg attttgttta cttctcatga ttcttacttt gtcgagcgta
     1861 cggctgatga agtttatgaa ataaaagggc agaaaataaa aaaagtactt acgagaaatt
     1921 tttaaatgtg ctacgatgat tttcgtgaca aatttaaagg agtggtacag attgcctaca
     1981 aataaaaaag aaggaataat ttttacaaca atgatgtgtt ttttgatggt cttggggatg
     2041 agcgcgtata atttatggct gcatcaagat cttcatttat caagtttgtt gattggtcta
     2101 gtgcctggat tcgtggtggc tttcattttg gatgtcttca tcatcggtgt gctggcgaaa
     2161 aaaattgcct ttaaactacc agtaaatcca gaaagcaagg tccaaatgat cttaactatt
     2221 tcatgcttga tggttttagg aatggtgaca tgtatgtctt tattcggtgt gatcatggaa
     2281 caaggaatac ctgatcaatt attctctgct tatatgaatg cttggaaaat gaatgtgatt
     2341 gccgctttac cgttgcagtt gatcatcgtt ggcccgcttt caagaag
//
LOCUS       DANKKG010000396         2319 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.guided.230, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000396 DANKKG010000000
VERSION     DANKKG010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.guided.230"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..1212
                     /locus_tag="PW004_003052"
     CDS             <1..1212
                     /locus_tag="PW004_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HDL2727927.1"
                     /translation="FGGFGLAFGRDIGDVIGNPADFFLMRHVTSFPNMFHGATIPFLM
                     FFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQMG
                     FRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWFGF
                     NSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAAIT
                     PCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIGIF
                     ASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEEGL
                     DFFFFGFLAMTISMVVSLYEYPTFATSGFSLVFFLLLGGLLWFIPVALCAAEMATVKG
                     WEKGVSQTKRY"
     gene            1607..2080
                     /gene="dfrF"
                     /locus_tag="PW004_003053"
     CDS             1607..2080
                     /gene="dfrF"
                     /locus_tag="PW004_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HDL2727928.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            2207..>2319
                     /locus_tag="PW004_003054"
     CDS             2207..>2319
                     /locus_tag="PW004_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HDL2727929.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLI"
BASE COUNT          704 a          342 c          536 g          737 t
ORIGIN      
        1 tttggtggat tcggattggc ctttggacgg gatatcggcg atgttattgg aaacccggca
       61 gatttctttt tgatgagaca tgttacaagc ttccccaaca tgtttcatgg tgcaacgatt
      121 ccatttttaa tgttctttct ttaccaactg atgtttatcg tgattaccgt tcctttgatg
      181 acaggagctt ttgcagggag attgaattta aaaggctata ttatacttgt tattttttgg
      241 aatctgctga tttatttccc tgtttgccac tggatatggg gtggcggatt tttggatcag
      301 atgggattta gagatttcgc tggtggagca gtcatccaca cgacagctgg attcggctct
      361 ttagcatgta tcctcacact tggtcaaaga aagattcctt taggagaaag aagtcagcat
      421 agcagtctta tggcagctgc tatcggtaca ggactgcttt ggtttggctg gttcggtttt
      481 aattccggtg gtgcacttcg agcagatgaa caagcagtca atgcatttgg ttcaacattc
      541 atcgctcttg catttgcgat gattacttgg ctgatcattg caaaagtaaa aggaaatggc
      601 tttgattttg tcgacgtact aacaggatcc gttgctggat tggcagccat tacgccttgt
      661 gccggttatg tagaagcaaa aagtgccatg ctgattggga tcattgcggg tatcgtctgt
      721 catgctgctg ttgattttcg taaaaagaaa caatgggatg atgcacttga tgtgtggggt
      781 gttcatggca tgggcggatt cacaggtacg attctgatag ggatctttgc atctaacagc
      841 catttactaa cgaatccaag cagctggtac ttcttaggga ttcaagtgat tggtgtcatc
      901 attactgccg tgtatgccta tgtgctgaca acagtgattt taaaatcggc gatgcacttt
      961 acaacgatca ctactacaaa agaagaacaa gaagaaggat tggacttttt cttctttggc
     1021 tttttggcta tgaccatctc aatggtcgtt tcattatatg aatatcccac attcgcgacc
     1081 tcaggcttca gcttagtatt tttcttgtta ttaggcggtt tgctatggtt tatccctgtg
     1141 gcattgtgcg cagcagagat ggcaacggtg aaaggatggg aaaaaggtgt ttcccaaaca
     1201 aaaagatatt aagaaacagc ccatgtttac ctgcttgcaa acaaaagaat attgctacta
     1261 gactaagaga tactacataa gtgtgttggt atctcttttt tgttgccgtg aaacagcaga
     1321 ctatttcatt tctgcttgat taccttaaaa cttattagtc tatggtttgt caagggcttg
     1381 ttgcgtgaga aatgctgaat atcacccttt acaaaacata ggctgataag taacttttca
     1441 aaagcagaaa agaaatggca atgcttataa caatatgata taatgcagat gaagacaaaa
     1501 aaggaatttg tcgagctggt tagttcgaga taattttgag tttgttaaga tgtggtgcac
     1561 ttgatggaat cacaggtacg acatcatatt atggaggatg aaatagatga taggtttgat
     1621 tgttgcgagg tcaaagaata atgttatagg caagaatggt aatataccat ggaaaataaa
     1681 gggagaacaa aagcaattta gagagttaac aacgggtaat gtggttatta tggggcgaaa
     1741 gtcttatgaa gaaatcggtc atccgttgcc taatagaatg aatattgttg tttccaccac
     1801 aacagagtat caaggagata atttagtttc agttaaatca ttagaagatg cattattatt
     1861 ggctaaagga cgagatgtat acatatctgg tggatatgga ctatttaagg aagctttgca
     1921 aatagtagat aaaatgtata tcacagaagt agatttaaat attgaagatg gagatacatt
     1981 ctttccagaa tttgatatca atgattttga agttttgata ggggaaacac ttggtgagga
     2041 agtgaaatat acgagaacat tttatgtaag gaaaaaatga attgagtaaa ttttggattt
     2101 agggtgtttt cataaatata ttgcttttta tgcatatata aattgttgta agacaaattc
     2161 cagtttgtat ggggcgataa aatcagaatt tgagagagga ttgaatatga ataaggaatg
     2221 gtctgaactt aataaaacaa tgcaggcaca aataaaaaag aaagatactt ataagagggg
     2281 tattgatact ttacttactt tacgaagtca gttaataca
//
LOCUS       DANKKG010000397         1863 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.guided.250, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000397 DANKKG010000000
VERSION     DANKKG010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.guided.250"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..1311
                     /locus_tag="PW004_003055"
     CDS             <1..1311
                     /locus_tag="PW004_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HDL2727930.1"
                     /translation="ESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYH
                     YPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAI
                     RAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVD
                     SIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIE
                     YSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISE
                     CTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSC
                     NHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIE
                     KAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
     gene            complement(1441..>1863)
                     /locus_tag="PW004_003056"
     CDS             complement(1441..>1863)
                     /locus_tag="PW004_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2727931.1"
                     /translation="WQRCQVHFLRNIFTTIPKKNSKSFREAVKGIFKFTDINLAREAK
                     NRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRLKSTNLIERLNQEVRRREK
                     IIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT          729 a          181 c          323 g          630 t
ORIGIN      
        1 gaatcattaa aaaaacatta tacagagcct tgggaagatg aagtttttag agtaattatt
       61 gaatataaca atgttcctat tggatatgga caaatatata aaatgtatga tgagttatat
      121 actgattatc attatccaaa aactgatgag atagtctatg gtatggatca atttatagga
      181 gagccaaatt attggagtaa aggaattggt acaagatata ttaaattgat ttttgaattt
      241 ttgaaaaaag aaagaaatgc taatgcagtt attttagacc ctcataaaaa taatccaaga
      301 gcaataaggg cataccaaaa atctggtttt agaattattg aagatttgcc agaacatgaa
      361 ttacacgagg gcaaaaaaga agattgttat ttaatggaat atagatatga tgataatgcc
      421 acaaatgtta aggcaatgaa atatttaatt gagcattact ttgataattt caaagtagat
      481 agtattgaaa taatcggtag tggttatgat agtgtggcat atttagttaa taatgaatac
      541 atttttaaaa caaaatttag tactaataag aaaaaaggtt atgcaaaaga aaaagcaata
      601 tataattttt taaatacaaa tttagaaact aatgtaaaaa ttcctaatat tgaatattcg
      661 tatattagtg atgaattatc tatactaggt tataaagaaa ttaaaggaac ttttttaaca
      721 ccagaaattt attctactat gtcagaagaa gaacaaaatt tgttaaaacg agatattgcc
      781 agttttttaa gacaaatgca cggtttagat tatacagata ttagtgaatg tactattgat
      841 aataaacaaa atgtattaga agagtatata ttgttgcgtg aaactattta taatgattta
      901 actgatatag aaaaagatta tatagaaagt tttatggaaa gactaaatgc aacaacagtt
      961 tttgagggta aaaagtgttt atgccataat gattttagtt gtaatcatct attgttagat
     1021 ggcaataata gattaactgg aataattgat tttggagatt ctggaattat agatgaatat
     1081 tgtgatttta tatacttact tgaagatagt gaagaagaaa taggaacaaa ttttggagaa
     1141 gatatattaa gaatgtatgg aaatatagat attgagaaag caaaagaata tcaagatata
     1201 gttgaagaat attatcctat tgaaactatt gtttatggaa ttaaaaatat taaacaggaa
     1261 tttatcgaaa atggtagaaa agaaatttat aaaaggactt ataaagattg attatataat
     1321 atatgaaaag ctattataaa agacattagt attaaatagt ttaaaaaaat gaaaaataat
     1381 aaaggaagtg agtcaagtcc agactcctgt gtaaaatgct atacaatgtt tttaccattt
     1441 ctacttatca aaattgatgt attttcttga agaataaatc cattcatcat gtaggtccat
     1501 aagaacggct ccaattaagc gattggctga tgtttgattg gggaagatgc gaataatctt
     1561 ttctcttctg cgtacttctt gattcagtcg ttcaattaga ttggtactct ttagtcgatt
     1621 gtgggaattt ccttgtacgg tatattgaaa ggcgtcttcg aatccatcat ccaatgatgc
     1681 gcaagctttt gaatattttg gttgatcgat ataatcatga atcaatcgat ttttagcctc
     1741 acgcgctaag ttaatatctg tgaacttaaa aattccttta acagcttctc tgaaagattt
     1801 tgaatttttt ttaggaatgg tggtaaagat atttcttagg aagtgaactt ggcatctttg
     1861 cca
//
LOCUS       DANKKG010000398         1358 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.guided.286, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000398 DANKKG010000000
VERSION     DANKKG010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.guided.286"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            15..527
                     /gene="aac(6')-I"
                     /locus_tag="PW004_003057"
                     /pseudo
     CDS             15..527
                     /gene="aac(6')-I"
                     /locus_tag="PW004_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708502.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(6')-Ie family aminoglycoside
                     N-acetyltransferase"
     gene            558..>1358
                     /gene="aph(2'')-I"
                     /locus_tag="PW004_003058"
     CDS             558..>1358
                     /gene="aph(2'')-I"
                     /locus_tag="PW004_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021424053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HDL2727932.1"
                     /translation="MKYLIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTN
                     KKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTM
                     SEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEK
                     DYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDF
                     IYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPIETIVY"
BASE COUNT          566 a          103 c          234 g          455 t
ORIGIN      
        1 tagttgaaaa tgaaatatgt ataagaactt taatagatga tgattttcct ttgatgttaa
       61 aatggttaac tgatgaaaga gtattagaat tttatgatgg tagagataaa aaatatacat
      121 tagaatcatt aaaaaaacat tatacagagc cttgggaaga tgaagttttt agagtaatta
      181 ttgaatataa caatgttcct attggatatg gacaaatata taaaatgtat gatgagttat
      241 atactgatta tcattatcca aaaactgatg agatagtcta tggtatggat caatttatag
      301 gagagccaaa ttattggagt aaaggaattg gtacaagata tattaaattg atttttgaat
      361 ttttgaaaaa agaaagaaat gctaatgcag ttattttaga ccctcataaa aataatccaa
      421 gagcaataag ggcataccaa aaatctggtt ttagaattat tgaagatttg ccagaacatg
      481 aattacacga gggcaaaaaa gaagattgtt atttaatgga atatagatat gatgataatg
      541 ccacaaatgt taaggcaatg aaatatttaa ttgagcatta ctttgataat ttcaaagtag
      601 atagtattga aataatcggt agtggttatg atagtgtggc atatttagtt aataatgaat
      661 acatttttaa aacaaaattt agtactaata agaaaaaagg ttatgcaaaa gaaaaagcaa
      721 tatataattt tttaaataca aatttagaaa ctaatgtaaa aattcctaat attgaatatt
      781 cgtatattag tgatgaatta tctatactag gttataaaga aattaaagga acttttttaa
      841 caccagaaat ttattctact atgtcagaag aagaacaaaa tttgttaaaa cgagatattg
      901 ccagtttttt aagacaaatg cacggtttag attatacaga tattagtgaa tgtactattg
      961 ataataaaca aaatgtatta gaagagtata tattgttgcg tgaaactatt tataatgatt
     1021 taactgatat agaaaaagat tatatagaaa gttttatgga aagactaaat gcaacaacag
     1081 tttttgaggg taaaaagtgt ttatgccata atgattttag ttgtaatcat ctattgttag
     1141 atggcaataa tagattaact ggaataattg attttggaga ttctggaatt atagatgaat
     1201 attgtgattt tatatactta cttgaagata gtgaagaaga aataggaaca aattttggag
     1261 aagatatatt aagaatgtat ggaaatatag atattgagaa agcaaaagaa tatcaagata
     1321 tagttgaaga atattatcct attgaaacta ttgtttat
//
LOCUS       DANKKG010000399         1010 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.guided.313, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000399 DANKKG010000000
VERSION     DANKKG010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.guided.313"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            <1..774
                     /locus_tag="PW004_003059"
     CDS             <1..774
                     /locus_tag="PW004_003059"
                     /inference="COORDINATES: protein motif:HMM:NF019310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2727933.1"
                     /translation="AISGVVFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREV
                     GVIAVAKNDLKDSYTFLKKEKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPD
                     TLYGFSQSALSLGGLCGGILTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAI
                     VSYWVVTVMCFVAMGVSTMFVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMV
                     YGILFDVFIQETWVVMFVASIASIMISIYSKRIFQKLEI"
BASE COUNT          303 a          134 c          184 g          389 t
ORIGIN      
        1 gctataagcg gagtagtttt tcaattttat ggtatcaatc ccattttaat tgttagtatt
       61 atatgtttta ctttttcagc cattatggaa atttttatcc atattccaca ccaaaaaaga
      121 cctcgtgaag taggtgtaat tgcagttgct aaaaacgatt taaaagatag ttataccttt
      181 ttgaaaaagg agaaacccat ttttatatcc gtaatctttt tggtttgcat tttcaatttg
      241 gttttaagtg ctgttttaat tgttggtctg cctatcttaa tttctgaagt tttaaaaatg
      301 ccagatacac tatatggttt ttcacaatca gcattatcat taggtggttt atgtggtggt
      361 attttaacag ctcttgttgc ttcaaagtta aaactaaaaa acctttatac cttgttactt
      421 atttgttctg tttctgtttt acttatggga atttcgctat ttttagaatt gcctgcaata
      481 gtttcctact gggtagttac agtgatgtgc tttgtggcta tgggtgtatc tactatgttt
      541 gttgttcaaa tttacactct tgttcagaca caaacaccac cgcaattagt aggaaagata
      601 atggctacgc tggtagcagt tgctatgtgc ggtcaaccta ttggacaaat ggtttacggt
      661 attttattcg atgtatttat acaagaaaca tgggttgtta tgtttgtagc ttctattgct
      721 tcaatcatga tttcaatata ttcaaaaaga atttttcaaa agttagagat ataatacaag
      781 gggttaaata gtatgttagg ctaaaaacaa tatataaaag aaaatttgaa atctgctaaa
      841 aggatagtgt tataaaagaa agagtaatat tgcagtattt aaggctcgca caaatatatg
      901 ttttgtgtgg gcttttttct taccattttt ctgtatttct aaagggtata aacggtattc
      961 cgtttatata tttcagcatt agcagaaagg gggtgagatt atgaggacat
//
LOCUS       DANKKG010000400          788 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.guided.338, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000400 DANKKG010000000
VERSION     DANKKG010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.guided.338"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            102..599
                     /gene="dfrG"
                     /locus_tag="PW004_003060"
     CDS             102..599
                     /gene="dfrG"
                     /locus_tag="PW004_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HDL2727934.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
BASE COUNT          294 a           91 c          161 g          242 t
ORIGIN      
        1 aaaaaattgc ttattcaact aacgggcagg ataatgagaa aaggtttttt gatagaaata
       61 agaaattaga tggtaaatta gttaaaaagg ctgtgttata aatgaaagtt tctttgattg
      121 ctgcgatgga taagaataga gtgattggca aagagaatga cattccttgg aggattccca
      181 aggactggga atatgttaaa aatactacaa agggacatcc gataatatta ggtaggaaga
      241 accttgaatc aatcggaaga gccttacctg acagaagaaa tattattctg acgagagata
      301 aggggtttac ctttaatggt tgtgaaattg ttcattcaat agaagatgtt tttgagttat
      361 gtaaaaacga agaagaaatt tttattttcg gaggagaaca gatttataat ttgtttttcc
      421 cttatgttga gaaaatgtac atcacaaaaa tacatcatga attcgaagga gatacttttt
      481 ttccagaagt gaattatgag gaatggaatg aggtatttgc ccaaaaaggg ataaagaatg
      541 ataaaaatcc gtataactac tattttcatg tatatgaaag aaaaaactta ttgagttaac
      601 gggtgcgtta attgaagaag aacagtaaag ataaagcaca agagcttggg attactccaa
      661 gctctttttg tcatttgaaa taaattatat ctttccaatc aaattgttta tctgtaatcc
      721 ctaatctttg agcaggtgtc aaaacctttt tatccgctga tttaaaaggg cgacaaaagt
      781 tgtagtaa
//
LOCUS       DANKKG010000401          712 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538785-rid17237793.guided.349, whole genome shotgun
            sequence.
ACCESSION   DANKKG010000401 DANKKG010000000
VERSION     DANKKG010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538785
            Sequence Read Archive: ERR10688348
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638110.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:05:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 2,995
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 27 of 62
            Pseudo Genes (internal stop)      :: 14 of 62
            Pseudo Genes (multiple problems)  :: 9 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538785-rid17237793.guided.349"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Urine, midstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-11-02"
     gene            99..647
                     /gene="aac(6')"
                     /locus_tag="PW004_003061"
     CDS             99..647
                     /gene="aac(6')"
                     /locus_tag="PW004_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HDL2727935.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          257 a          110 c          163 g          182 t
ORIGIN      
        1 gaatagagat actgattggt agctaaagat ttttgagcga gaacatttcg ttttttcaaa
       61 aggactgtta gaataagcaa gaacaagggg gaagacgtat gataatcagt gaatttgacc
      121 gtaataatcc agtattgaaa gatcagcttt ctgatttact gagactgact tggccggaag
      181 aatatggaga cagctcggca gaagaagtag aagaaatgat gaatccagaa cgaatcgcgg
      241 tagcagcggt agaccaagat gagttagtag gatttattgg tgcaatccct caatacggta
      301 tcacaggttg ggaattgcat ccattagttg tagaaagctc ccgacgaaag aaccaaatag
      361 gtactcgatt agtcaattac ttagaaaaag aagtagcttc cagaggagga atcacgattt
      421 atttaggtac ggatgattta gaccatggaa caacgttaag tcaaacggac ctgtatgagc
      481 atacatttga taaagtggct tccatccaga accttcgtga acatccgtat gaattctatg
      541 aaaaattagg ttataaaatc gtaggtgtct taccaaatgc aaatggctgg gacaaaccgg
      601 atatttggat ggcaaaaacg attattcctc gaccaaattc tcaataataa aaagaggttg
      661 tgacagaagt aatcagcttc aagaaataag aaggaattta cgaaaattac tt
//
